LOCUS       DATCXU010000001         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1097609,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000001 DATCXU010000000
VERSION     DATCXU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097609"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VIR04_00005"
     CDS             <1..831
                     /locus_tag="VIR04_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY9459160.1"
                     /translation="ADPSLVVGPALAHFGWDRNDWHRLGCATMAGHLLECGTQVTGGY
                     FAIPGLKDVDGMENLGFPIASINQSGEISITKAPGTGGAVTEQTVKEQLLYEIHNPAA
                     YLTPDVTADISRVTVAQTASNCVAVGNVTGHERPEELKVTICHKGGWLAEAEISYAGV
                     QAEARARLAADIVQRRIGSQLKLRVDLIGSSSILGDDHGTYLHHKAPGSSQDIRLRVA
                     ATHPQKDIAQRVLREVTALYTCGPAGGGGVRTSLHPRLNSISSTMPRDLAPAGWSFLA
                     "
     gene            842..1255
                     /locus_tag="VIR04_00010"
     CDS             842..1255
                     /locus_tag="VIR04_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459161.1"
                     /translation="MQTSSTLTVPLYRLAHSRAGDKGDISNISVIAWKADYFELLKEQ
                     LTESAVLHWFAYRQPKSVRRYLVESIHAMNFVLDGVLDGGVNDALNLDTHGKSLSFFM
                     LDFPIQVSAQSASALPDIKLAHYITGDNPSCSGIL"
     gene            1237..2211
                     /locus_tag="VIR04_00015"
     CDS             1237..2211
                     /locus_tag="VIR04_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459162.1"
                     /translation="MFRHLISKKSLVALAIGVCLPWVTQAQDYPSHSITFVVPFGAGS
                     ATDQLARAIGQGITEETRQVVIVENKAGASAMIGASDVARAAPDGYRVLITTNTSQAA
                     NPHLYKKLSYDPVGDFEPVSLLGTGGQIMVVNPASTVKTVQDFIALAKKEPGKITFGS
                     GSSSSRVAGELFQQMAGVELLHVPYKSNPAAITDLLGGQIDMMMADMATGLPQVKAGK
                     LRALGVTTMKPSPLTPDVPTIDKSGVPGYEMGYWFAAYVPAKTPAAVVSKLNALLINA
                     VKRPAAHQFYSQTGTEPVTSTPAELANFQKNETEKWGVIIRKAGIEPS"
     gene            2316..2849
                     /locus_tag="VIR04_00020"
     CDS             2316..2849
                     /locus_tag="VIR04_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY9459163.1"
                     /translation="MAVPAFDYETSLIACAKGDQSAFQELYKQESSHMLALCAKMLSQ
                     RADAEEIVRDAFVLIWKNASNYDSKMGTARAWMYSIMRYRVLNRLRQSGRIMPVDSEW
                     VDTLPDAIVSANTEQANGLTQCVAKLDDAQRRPILMAFYNGFTYDQIAARLKTPAAEV
                     KADVRAGLNSIREFAQA"
     gene            2846..3595
                     /locus_tag="VIR04_00025"
     CDS             2846..3595
                     /locus_tag="VIR04_00025"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY9459164.1"
                     /translation="MTIQHSSDSDLLIAEYTLGLLNLQETARAQTLLGKDPEAVAIAL
                     EWEGRFLDLVDQLPPIEPPAQLLQRIQATLGMEITLIKRPNAVLKASKKVVCAIWRGL
                     WFWRGLSVALFAIALAYAAKPNLAVIEAPPIVRVAILQAPGQSSTPGWVATLDKKNNL
                     SLSPKVHTDIPADATVRLWTHNDSAPQPRSLGAIEPNQPITIPASSLGVMGQDQIFEM
                     TLEPKSGSSAASPTGPVLFIGRMITLDQQPK"
     gene            complement(3618..4016)
                     /locus_tag="VIR04_00030"
     CDS             complement(3618..4016)
                     /locus_tag="VIR04_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY9459165.1"
                     /translation="MSSSETPVTLPPIPAKRYFTIGEVSDLCCVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRDLLYEQGFTISGARNRLGDSREIPHDQDAAVRL
                     SGQELQSLRADLLAARDDLARALGDAPGGG"
     gene            complement(4103..>4230)
                     /locus_tag="VIR04_00035"
     CDS             complement(4103..>4230)
                     /locus_tag="VIR04_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY9459166.1"
                     /translation="NPKTGEIIPIAARRVVTFHASQKLKSEVEQAGADPAHPSTD"
BASE COUNT         1009 a         1213 c         1138 g          870 t
ORIGIN      
        1 gccgatccgt cgctcgtcgt gggcccggca ttagcccact tcggctggga tcgcaatgat
       61 tggcatcggc tgggatgcgc caccatggct ggccatctat tggaatgcgg cacgcaagtg
      121 actggcggat attttgcgat acccgggcta aaagatgtcg atggtatgga aaacctgggc
      181 tttccaattg ccagcatcaa tcagtcaggc gaaatttcga tcaccaaagc gccaggtacg
      241 ggcggagctg tcaccgaaca aacggtcaaa gaacaattac tgtacgagat tcacaatcca
      301 gccgcgtatt taacgcccga tgtcacggcg gatataagcc gggtcactgt ggcacaaacg
      361 gcaagcaact gcgttgctgt cggaaacgtc accggacacg aacggcccga agagctcaaa
      421 gtgactattt gccacaaagg cggctggttg gccgaagctg aaatctcgta cgcgggggtt
      481 caggccgaag cgcgggcgcg cctggcagcg gacattgtcc aacgtcgcat cggttcgcaa
      541 ctgaagttac gtgtcgatct aatcgggtca tcaagcattt tgggcgatga ccatggaact
      601 tatctgcatc ataaagcgcc aggcagtagt caggatatac ggctacgcgt tgccgcaacc
      661 cacccgcaaa aggacatagc gcaacgcgtg ctgcgcgaag tgacagcgct gtatacctgt
      721 ggtcctgccg gcggcggagg tgtgcgcaca tcgctgcatc cccggttgaa tagtatttcg
      781 agcacaatgc cgcgggacct ggctcctgca ggctggagct tcctggctta agacaagaat
      841 tatgcaaaca tccagcacgt taaccgtacc tctataccgt ttggctcaca gccgggcggg
      901 agacaagggc gatatttcca atatcagcgt catcgcgtgg aaagccgact atttcgaact
      961 ccttaaagag cagctcactg aatcggccgt actccactgg tttgcatacc gccagcccaa
     1021 aagcgtgcga cgctatctgg tcgaatcaat tcatgccatg aatttcgttc ttgacggcgt
     1081 tctcgatggc ggcgttaacg acgctttaaa tctggacact catggcaagt cattatcgtt
     1141 cttcatgctc gacttcccca tccaggtgag cgcgcagtca gcgtccgccc tccccgatat
     1201 aaaactcgcc cattacatta caggagacaa cccatcatgt tccggcatct tataagcaaa
     1261 aaatccctgg tggcgctcgc catcggcgtc tgtctgccgt gggtcactca agcccaggac
     1321 tacccaagcc actccattac atttgttgtg ccattcggcg cgggaagcgc gacggatcaa
     1381 ctcgccaggg cgataggaca gggaatcacc gaagagacca ggcaagttgt catagttgaa
     1441 aacaaagcag gggccagcgc aatgatcggc gccagcgacg ttgcccgcgc cgcgcctgac
     1501 ggataccgcg tgcttatcac cactaatact tcgcaagccg ccaaccccca tctatacaaa
     1561 aagctctcct acgatcccgt tggcgatttt gagcccgttt ccctgcttgg aaccggcggg
     1621 cagatcatgg tggtcaaccc tgcttcaacc gtcaagactg ttcaagattt cattgcgctc
     1681 gccaaaaagg agccggggaa aatcacgttc ggtagcggca gttccagtag ccgcgtggca
     1741 ggagaactat ttcaacaaat ggccggtgtg gaactgctgc atgtgccata taaaagcaac
     1801 ccggccgcca tcacggacct gcttggcgga caaatcgaca tgatgatggc ggacatggcc
     1861 accggcctgc ctcaagtgaa agccggcaaa ctgcgcgccc ttggcgtaac caccatgaaa
     1921 ccatcccctc tgactcccga cgtgccgact atagacaagt ccggtgtgcc cggatacgaa
     1981 atgggttact ggtttgcggc gtacgttcca gccaaaacgc cggctgccgt cgtgtcgaaa
     2041 ctgaacgcgc tattaatcaa cgcggtcaaa cggcccgctg cccatcagtt ttatagccaa
     2101 accgggaccg agccggtcac atcgaccccg gccgaactgg ccaattttca gaagaatgaa
     2161 acagaaaaat ggggcgtcat tatccgcaaa gcagggattg aaccgtcata ggcgttcgta
     2221 acatcttatt acgtcacgcc agcgcgctgg aacttttacc tctgcgaaca cctaatacaa
     2281 taacgattcc cacgacggga taaaccggag aatgtatggc tgttccagcg tttgattacg
     2341 aaaccagtct gattgcctgc gcgaaggggg atcaatccgc gtttcaagag ttatacaaac
     2401 aagaatcctc tcatatgctg gcgctttgcg ccaaaatgct gtcccaacga gccgatgccg
     2461 aagaaatcgt acgcgatgcg tttgtcctga tttggaaaaa tgcgtcgaat tacgattcaa
     2521 aaatggggac cgcccgcgca tggatgtaca gcatcatgcg ctatcgcgtc ctgaaccgtc
     2581 tgcgtcagtc cgggcgcata atgcccgtcg atagcgaatg ggtcgacacc ttgcccgatg
     2641 ccatcgtatc cgccaatacc gaacaggcta acggtttaac gcaatgtgtg gcgaaactcg
     2701 acgatgctca acgccgccct atcctgatgg ccttttataa tgggttcacg tacgatcaaa
     2761 ttgccgcgcg cctcaaaacg ccggctgccg aagtcaaagc cgatgtgcgc gctggtttga
     2821 actccatccg ggagttcgcg caagcatgac tatccagcac tcaagcgatt cagatctttt
     2881 gatcgccgaa tacactttgg gcctgctcaa tctgcaggaa acggcacggg cgcaaacatt
     2941 gctggggaaa gatccagaag ctgtcgccat cgcgctagag tgggaaggcc gctttctgga
     3001 tctggtcgat caacttcctc ccatcgaacc accggcgcaa ttattgcagc gcatccaggc
     3061 cacgctgggc atggaaataa cactgataaa acgtcccaac gccgtcttga aggcgagcaa
     3121 aaaagttgtg tgcgccatct ggcgtggact ctggttctgg cgcgggctta gcgttgccct
     3181 gtttgccatc gccctggcct acgcggccaa acccaatcta gccgtcatcg aggcgccgcc
     3241 gattgttcga gtagccatat tgcaggcgcc cgggcaaagc tcaactccgg ggtgggtggc
     3301 gactcttgac aagaaaaaca atctatcgtt aagcccaaag gttcataccg acatccctgc
     3361 cgatgcgacg gtgcggctat ggacgcataa cgacagcgct ccacagccgc ggtctttagg
     3421 cgcgatcgag cccaatcagc caatcaccat acccgcttca tcgctgggcg taatgggcca
     3481 ggatcaaatt tttgaaatga cactggaacc caaaagcggc tcgtctgctg ccagcccaac
     3541 cggacccgtc ttgttcattg ggcgcatgat tacgcttgac cagcagccca agtgacacgc
     3601 aatccggcaa tggggcgtta accgccgccg ggcgcgtcgc ccagcgcacg ggccaggtcg
     3661 tcgcgcgcag ccagcagatc cgctcgcaag gattgcagtt cctggccgct taaacgcacc
     3721 gccgcgtcct gatcatgggg gatctcgcgc gaatcgccca aacggttgcg cgcgccgcta
     3781 atggtgaagc cctgctcgta taacaaatca cggatccggc gaattagtaa aacctcgtga
     3841 tgctggtaat aacgtcggtt tccacggcgc tttaccggct tcaactgtgt aaactcctgc
     3901 tcccagtatc gcagcacgtg gggtttgaca cagcataaat cgctgacttc gccgatcgtg
     3961 aagtaacgtt tcgcgggaat cggaggtaag gtgactgggg tttcgcttga gctcatgcgc
     4021 ggattgtaga cggaaaaatt aacaacacaa agactgggtc gaatgcggga gcaaaaacac
     4081 gttcgggcga cagcgcgcgg agtcagtcag tacttgggtg cgctgggtca gctcccgctt
     4141 gttcaacctc gcttttcaat ttttgactgg catgaaatgt gaccacgcga cgcgcggcaa
     4201 ttggaatgat ctcgcccgtc ttgggattgc
//
LOCUS       DATCXU010000002         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1178227,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000002 DATCXU010000000
VERSION     DATCXU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178227"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VIR04_00040"
     CDS             <1..1629
                     /locus_tag="VIR04_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY9459167.1"
                     /translation="PTISAGFAAGSYGTYRYGAKANGVANLGTSKLDYVLSGTRFTTQ
                     GYRDHSSTSKNLGNAKLGLQLDDKSKLTIIANSVDLVADDPMGLSRQQFEDDPRGVTP
                     GALTFNTRKTVKQTQGGLIYDREVNANNALRLMVYYGQRKTTQFQSIPVGTQLNPLQA
                     GGVIDLSREYMGADLRWTSRLSIAGKPLTLIGGLAYDELAEQRKGYQNFIGSGPAQQL
                     GVQGALRRNETNKVWNIDPYLQASWQFAPRWTLDAGLRYSTVRFNSNDHFIVAGNPDD
                     SGSVNYRKALPVGSLRYAATPNIDLYASIGRGFETPTLNEISYRPNGASGLNFDLQPS
                     VNTSVEVGAKARIAGGLLTAALFQTNTNDEIVTASNDNGRSIFQNAGRTRRNGFELGW
                     NGEIARNWRTEFAYTWLDAKYRDDCPPSVCSSPIQAGARIPGIARQSVYASLGWVPPE
                     GWRAGVEARYLSKIYVNDGNSDAAPGYFVAALNVGYVWRTGPWELSTFARVDNVFDRH
                     YAGSVIVNDGNARYFEPAPGRNWTAGVNVAYSFR"
     gene            1831..3090
                     /locus_tag="VIR04_00045"
     CDS             1831..3090
                     /locus_tag="VIR04_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY9459168.1"
                     /translation="MSAIVVGLAFGALAGGAFGQTPTTPNNLQKLENMESTGTSLVME
                     SVPQTGKTADAIKRNLQNIKLPDGFKIELYAVAPGARHMAIGPSSGIVFIGTRSTKIW
                     AATDRTKSRVADDVVRFAPSVSFKIPNGVCFSPDGILYTVEQNRVLAFPAAEFFYEGP
                     DVAAGIVVPQGKLIPPTEESYNHTARTCRIGPDKKLYITMGNPFNVPPKEKLDLYNKT
                     GMMGIIRMDTDGKNREVFAHGVRNSVGMDFNPKDDTLWFTDNQVDGMGDDIPPGELNH
                     ATQMGQNFGFPYYGGGKVRTVEYKNDPPPDDAIFPQVEMAAHAADLGMSFYTGTMFPE
                     KYRGGIFSAQHGSWNRTTPVGARIMFTSLKDDGTVDKTEVFADGWLTPNHEYLGRPVD
                     VEVMRDGSLLVSDDYAGAVYRISYEGK"
BASE COUNT          748 a          897 c          863 g          626 t
ORIGIN      
        1 ccgacgattt cggctggatt tgcggcaggc agttatggca cgtatcggta cggggcaaag
       61 gcaaacggcg tcgccaactt gggaaccagc aagctggatt acgtactaag cggcacgcga
      121 ttcaccacgc aggggtatcg cgatcacagc agcaccagca aaaacctggg caatgccaaa
      181 ctgggcttgc agcttgacga caaaagcaag ctgacgatca ttgccaatag cgttgacctg
      241 gtggccgacg acccaatggg attgtcgcgg cagcagtttg aagatgatcc gcgtggcgtc
      301 acgccaggcg ccctaacatt caatacgcgt aaaacggtca agcaaaccca gggcggtttg
      361 atttacgatc gcgaagtaaa cgccaacaat gcgttgcgcc tgatggtgta ttacggccag
      421 cgcaagacaa cgcaatttca atccatccct gtcggaaccc agcttaatcc gctacaagcg
      481 gggggcgtca tcgacctttc tcgcgaatac atgggcgccg accttcgctg gacatccagg
      541 ctatccatcg ccggaaagcc cttgacactg atcggaggac ttgcctacga cgagctggcg
      601 gaacaacgca agggctacca aaactttatt ggaagcggac cggcgcaaca actgggagtc
      661 cagggggcgc tgcgccgcaa cgagacaaat aaagtctgga atatcgatcc ctacttgcaa
      721 gcctcatggc aattcgctcc ccgatggacg ctggatgcgg ggcttcggta cagcacggtg
      781 cggttcaact ccaacgatca ctttatcgtt gccggcaatc ccgacgacag tggctcggtg
      841 aattatcgca aggcgctgcc ggtgggatcg ctgcgctatg ccgctacgcc caacatcgac
      901 ctatacgcgt cgatcgggcg cggatttgaa actcccacgc taaacgaaat ttcgtaccgg
      961 ccaaacgggg cctcggggtt gaatttcgac ctgcagccct ccgtcaatac cagtgtcgaa
     1021 gttggcgcca aagcacgcat cgcgggcggc ttgcttacgg ctgcgctatt ccagacaaat
     1081 acgaacgacg aaattgtcac cgcgagcaac gacaatggcc gctcgatttt tcaaaatgcg
     1141 gggcgcacgc ggcgcaacgg attcgagctg ggatggaacg gagaaatcgc ccgcaactgg
     1201 cgcacggaat tcgcgtacac ctggctcgat gcgaaatacc gcgatgattg cccgccctcg
     1261 gtctgctcaa gccccattca ggcgggcgcg cgcatccccg gcatcgccag gcaatcggtt
     1321 tacgcctcgc tgggttgggt acccccagaa ggctggcgag caggcgttga agcgcgctat
     1381 ttaagcaaga tttatgtgaa cgacggtaac agcgatgcag cccccgggta ctttgtggcc
     1441 gcgctgaacg tcggttacgt ctggcgcacc gggccctggg agctatccac cttcgcccgt
     1501 gtcgacaatg tgttcgaccg gcactatgcg ggttctgtca ttgtcaacga cggaaatgcc
     1561 cgctacttcg agcccgctcc aggacggaac tggacggcgg gagtcaacgt agcctattca
     1621 ttccggtgaa atttgttgta actttaatgt cgtcgccgaa ccccgcttgg gccatatgaa
     1681 ggactttaaa acgcgtttat tacgcgtaga ataaaaatcg ccttctggat gcattcaaaa
     1741 gatttcgggg ttaaaaaact gcccgcacag ggtggaaggt tcacagaact ttaataacac
     1801 tggagacgtc aaatgtattt caaaaaaagc atgtctgcaa tcgtggttgg tcttgcattc
     1861 ggcgcccttg ccggtggcgc gttcgggcag accccaacga cccccaacaa ccttcaaaaa
     1921 cttgagaata tggaatccac cggcacgtcg ctggtcatgg aatccgtgcc gcaaactggc
     1981 aaaaccgcgg acgcaatcaa acgcaatctg caaaatataa aactgccgga tggattcaag
     2041 attgagctct acgctgtggc gcccggcgcg cgccacatgg ccatcggccc ttcctcgggc
     2101 attgttttca tcggcacgcg ttcaaccaaa atctgggcag ccaccgaccg cacgaaaagc
     2161 cgagttgcgg acgacgtcgt gcgctttgct ccgtcggtca gtttcaaaat acccaatggc
     2221 gtttgctttt cgcccgatgg cattctgtac accgtggaac aaaaccgtgt gctggccttt
     2281 cccgccgccg aatttttcta cgaaggaccg gacgtagccg ccggtattgt cgtgcctcag
     2341 ggcaagctta ttccgccgac cgaagaaagc tacaaccaca ccgcgcgcac ttgccgcatc
     2401 gggcccgaca aaaaactgta catcaccatg ggcaatccct ttaacgttcc gcccaaggaa
     2461 aagcttgatc tttacaacaa aaccggcatg atgggcatta tccgcatgga cactgacggc
     2521 aaaaaccgcg aggtgttcgc ccatggcgtg cgcaactctg tcgggatgga tttcaacccc
     2581 aaagacgata ccttgtggtt caccgacaat caggttgatg gtatgggcga cgacatccct
     2641 ccgggcgaac tcaaccacgc gacgcaaatg gggcaaaatt tcggctttcc ctattatggc
     2701 ggcggcaaag tcagaaccgt ggaatacaaa aatgatcctc ccccggatga cgctatcttc
     2761 ccacaagttg aaatggcggc gcatgcggcg gatctgggca tgtcgtttta caccggaacc
     2821 atgtttccgg aaaaataccg cggaggaatc ttttcggcac agcatggctc atggaaccgc
     2881 accacgccgg tcggtgctcg cataatgttc acgagcctga aagacgacgg caccgtcgat
     2941 aaaactgaag tgttcgccga tggatggctt acgcccaacc acgaatacct tggccgtccg
     3001 gtcgatgtcg aggtcatgcg cgacggttcg cttttagtat cggatgacta cgcaggcgcc
     3061 gtttacagaa tctcttatga gggcaagtaa gcttcggcag gcgctgcatg gcggcgcgct
     3121 ggcactggtg tcgg
//
LOCUS       DATCXU010000003         4321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_234455,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000003 DATCXU010000000
VERSION     DATCXU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234455"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VIR04_00050"
     CDS             <1..1138
                     /locus_tag="VIR04_00050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9459169.1"
                     /translation="PVAGTLLADFGAEVIKVEQPGCGDTLRGLGPYLEGESLWWNVDG
                     RNKKSITIDLRQEKGQALLRRLVAKSDALVENFRPGTLEKWNSGYKQLAEVNPGLVML
                     SVSGFGQTGPLAQRAGYDRIALAFSGVMAITGYPDRPPVRIGTSMADYTTATLGAFAV
                     MMALYSRDANGGTGQQIDLALYEPMFRFTESLTVAYDRLGFVRERTGNVHYGAAPGKN
                     FETSDNRYLTLTISGDTLFKRLCHAIGRAELADDPAYATHKLRWDRVDELNEIVAAWV
                     KNNDVDVITSALDNYGIPFSIALTIAEIIEHPHYKARENIVTVDHPTLGPLKMQGIMP
                     KLSKTPGGPITPAPSLGQHNEEVFFELLGMGASEFRQLSDEGVI"
     gene            complement(1135..1869)
                     /locus_tag="VIR04_00055"
     CDS             complement(1135..1869)
                     /locus_tag="VIR04_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9459170.1"
                     /translation="MSDVLVRNEGEVTIISINRPGKLNALSNGVAHGLEAAFKEFDES
                     DQKVAILAGEGKSFTAGADINDFPEFWRCVPTVGITTQKPVIAAVQGWCVGGGVVMAA
                     MADLCVCAENTTFWYPEGKLGVSGGMICTLAARMPHKAAMEIMYLARKINARRAYEMG
                     LVNEVVPDGEQLNAALKMANELVALAPLVLRGIKRIVNDQILTKGPSETAAILTRDMQ
                     AMNNSVDFKEGKQAFAEKRQPKFVGR"
     gene            complement(1897..3039)
                     /locus_tag="VIR04_00060"
     CDS             complement(1897..3039)
                     /locus_tag="VIR04_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY9459171.1"
                     /translation="MGPLNGIRVIELEAIGPVPWAAMMLSDMGADVLRVDRAVLADRG
                     VTREKKYQLTERGRRSVTADLKDPKGVEAVLTLLSKADILLEGMRPGVMERLGLGPQE
                     CLARNPALVYGRMTGWGQTGPLSNVVGHDINYIGITGALHAIGLSDAPPVPPLNLVGD
                     FGGGGMLLALGVVCGVLEARHSGKGQVVDAAMVDGSISMMASIMGQYASGEWKDQRQS
                     NVLDGGAHFYGVYETSDNRYVAVGAIEPRFYSAFLKGLGLDPSDLPAQRDDTSWPALR
                     KKVADIFFTKTREQWRQIFEDTEACFTPVLSLAEVASHPHHVQRNAFVELDGVLHPAP
                     APRFSRTPGKIAGPPVERGVGGKQAVADWGFDPDDARLKGLGWSNR"
     gene            complement(3044..3388)
                     /locus_tag="VIR04_00065"
     CDS             complement(3044..3388)
                     /locus_tag="VIR04_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9459172.1"
                     /translation="MLNDWTEQLKNTRKAAGALSSATPKLVAAYQGLNAAQNGNGALD
                     AKTRELIALAVAVTTRCDSCIASHANAARQAGVTEAELSDALGTAIALNAGAAYVYSV
                     RAMEAFGQFKTE"
     gene            3719..4069
                     /locus_tag="VIR04_00070"
     CDS             3719..4069
                     /locus_tag="VIR04_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY9459173.1"
                     /translation="MIASTRAFLTAAALALSGFVAAPALAQTSATPTPSTSKAKTPQQ
                     ERMTTCNQQAKGKTGDERKSFMSSCLKGDTAAPDKKLSSSQQRMKTCNTEAGAKDLNG
                     DARKAFMSTCLKNK"
     gene            4114..>4321
                     /locus_tag="VIR04_00075"
     CDS             4114..>4321
                     /locus_tag="VIR04_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459174.1"
                     /translation="MKNASVSSPAFTAQSRQQALIAQEERFLDVEHQNPQSPYYRGST
                     EPTRPQRRRFMAGALAAIGATSLPS"
BASE COUNT         1020 a         1296 c         1088 g          917 t
ORIGIN      
        1 gcctgtagcc ggtacgttac tggcggattt tggcgccgaa gtcatcaagg tggaacaacc
       61 cggttgcggc gacacgctgc gcggtcttgg tccttacctt gaaggtgaaa gcctgtggtg
      121 gaatgtcgat gggagaaaca agaagtcaat taccatcgat ttacggcagg aaaaaggcca
      181 ggcgctgttg cggcgtcttg tggcgaaatc cgacgccctc gtcgaaaact tccgccccgg
      241 aaccctcgaa aaatggaact cgggatataa gcaactggcc gaagtaaatc cgggcctggt
      301 catgttaagc gtctcgggat tcgggcaaac aggtccactg gctcagcgcg ctggatacga
      361 ccgtatcgcc ctggccttca gcggcgtcat ggcaatcaca ggataccccg accgtcctcc
      421 ggtgcggatc ggcacatcca tggcagacta tacaacggcg acacttgggg cctttgccgt
      481 gatgatggcg ctatatagcc gcgacgcaaa tggcggcacc ggccaacaaa ttgatctggc
      541 cctgtatgaa ccgatgtttc gatttacgga atcgcttacg gtagcgtatg accgattggg
      601 gttcgtgcgc gaaagaaccg gcaatgtaca ttacggggcc gctcccggca agaactttga
      661 aaccagcgac aatcggtatt tgacgcttac tatttcgggc gacaccctgt tcaaaagact
      721 gtgccatgcc atcgggcgcg cggaactggc cgacgatcca gcctacgcca cgcacaaact
      781 gcgttgggac cgagtcgacg aattgaacga gatcgtggcg gcctgggtca aaaacaacga
      841 cgtcgacgtt ataacttcgg cgctggataa ctatggcatt ccgttttcaa tcgcgctcac
      901 tattgccgaa atcatcgaac acccgcatta caaggcgcgc gagaatatcg tgaccgtgga
      961 tcatcccacg cttggtccgc tgaaaatgca agggatcatg cccaaattat ccaaaacgcc
     1021 aggcgggccc ataacgccag cgccatccct gggacagcat aacgaagaag tgttctttga
     1081 gttgctggga atgggcgcgt cagaattcag acagttgtcg gacgagggcg ttatttaacg
     1141 cccgacaaat ttgggctgcc tcttctccgc aaaggcttgc ttgccctctt tgaaatcaac
     1201 actgttattc atagcctgca tatcacgcgt tagaatcgcc gcggtttcgg aaggcccctt
     1261 ggtcaggatc tggtcgttga caatacgctt gatcccacgc agcaccagcg gcgccaatgc
     1321 gacaagctca ttggccattt tcaaagcggc attcagttgt tcaccgtcgg gcactacctc
     1381 gttgaccagc cccatttcat aggcgcgccg cgcatttatc ttacgggcca aatacatgat
     1441 ttccattgcg gctttgtgcg gcatccgtgc cgccaatgtg caaatcatgc ctccgcttac
     1501 gcccaatttc ccttcgggat accaaaacgt cgtgttctcc gcacagacac aaagatccgc
     1561 catggcggcc atcacaacgc ctccgcccac gcaccagcct tgaacggcgg caatcaccgg
     1621 cttctgagtt gtgatgccca ccgtcggcac gcagcgccag aactccggga aatcgtttat
     1681 gtcggcgcca gcggtaaaac tttttccttc gcccgcaagg atagcgacct tttgatccga
     1741 ttcatcgaac tccttgaagg cggcctcaag accgtgcgcc acaccgttgc tcagggcgtt
     1801 aagcttgcct ggccggttta tcgatatgat cgtgacctcg ccttcgttgc gtacaagtac
     1861 atcactcatt ttttatctcc agattatgta aatgatttac cggttcgacc agcccaaacc
     1921 cttcaggcga gcgtcgtccg ggtcaaagcc ccagtcggcg accgcctgtt taccgccaac
     1981 gccacgctcc accggaggtc cggcgatttt accgggcgta cgcgaaaagc ggggcgccgg
     2041 ggcgggatga agaaccccat caagttcgac aaaggcatta cgctgaacat gatgcggatg
     2101 gcttgcaact tcagccagcg aaagcaccgg cgtaaaacag gcctcagtat cctcaaaaat
     2161 ttggcgccac tgctctcgtg tttttgtaaa aaaaatatcg gctacttttt ttctaagcgc
     2221 cggccaggag gtgtcatcgc gttgcgccgg caagtcggaa ggatcaagac ccagaccttt
     2281 cagaaatgcg ctgtaaaacc gtggctctat cgcccccact gcaacgtacc gattatccga
     2341 cgtttcgtat acaccgtaga aatgggcgcc cccgtccagc acattacttt ggcgctgatc
     2401 cttccattct cctgatgcat attgccccat gatggaggcc atcattgaaa ttgacccgtc
     2461 caccatcgct gcatcgacca cctgtccttt tccactatgc cgagcctcaa ggacgccgca
     2521 gacaacgcca agagcaagca gcataccgcc cccgccaaaa tcgccgacaa ggttcaaagg
     2581 cgggacagga ggcgcatccg acaacccaat cgcatgcagg gctccggtaa taccgatgta
     2641 attgatatcg tggccaacaa cattggacaa cggccccgtc tggccccacc ccgtcattcg
     2701 gccatagacc agggctggat tccgggcaag gcattcttgc ggccccaagc ctaatcgctc
     2761 catgacgcca ggccgcatgc cttcaagcaa aatatccgct ttggaaagca gggtcaatac
     2821 cgcttcgacg cccttgggat ctttcagatc ggccgtgacc gagcgccgtc cccgttcggt
     2881 aagctgatat ttcttttccc gcgttacgcc ccgatcagcc aaaaccgcgc ggtctacccg
     2941 aagcacatca gcgcccatgt cggacagcat catcgccgcc catggcacgg gcccgattgc
     3001 ttcaagttca ataaccctta tgccatttag cggtcccatt tattcactcc gttttgaact
     3061 gcccgaaagc ctccatagcc ctgactgaat aaacatacgc cgcgccggcg ttcagcgcaa
     3121 ttgccgtacc caaagcatcg ctgagttcgg cttcggttac cccagcctgg cgcgcggcgt
     3181 ttgcgtggga agcaatgcat gaatcgcagc gcgtcgtaac cgccacggca agagcaatca
     3241 gctcgcgcgt tttggcatcg agcgcaccat tgccattctg cgcggcattc aggccctgat
     3301 aggccgccac cagcttgggc gttgcggaag acaaggcgcc agcagccttt cgcgtattct
     3361 tcagttgttc ggtccagtca ttcaacatag atattcccaa aaatttttcc ccgaaagaaa
     3421 tatatcatgg ttttatatat cggtacaata gttccattat gtagaacacc tagttgactg
     3481 actaccttga atccaaagct ttcgcttcta tttcaggtga tataacgcgt accgtgtttt
     3541 cccgcaggct gaatgggctg ggggcctcgg tcgcaacatc cgattggaat ttgtcacttt
     3601 ccagccatcg cgctgtttca aaatacacaa tgtcatgcaa tttccgcctt gcgtggattg
     3661 acaagtgtcg acaccataaa cacgccacat tcatttctta cctgaaagag gtgtctttat
     3721 gattgcatca acccgcgcgt tcctcacagc cgccgcgctg gcgctaagcg gttttgtcgc
     3781 cgcccccgcg ctggcccaga cttcagccac gcccaccccg tcgacaagca aagccaaaac
     3841 gccccagcag gaacgtatga cgacgtgtaa ccagcaagcc aagggtaaaa ccggcgatga
     3901 gcgtaaatcg ttcatgagca gttgccttaa aggtgacacc gccgcgcccg ataaaaaact
     3961 gagctcgtcg caacagcgca tgaaaacctg caacaccgaa gcgggcgcca aggatctgaa
     4021 cggcgacgca cgcaaagcat tcatgagcac ctgcctgaag aacaagtagt accgagtggc
     4081 actaacgctg aaagtttgaa atggagcgga tccatgaaaa atgcatcagt aagttcaccg
     4141 gctttcactg cccaatcgcg acaacaagcc ctgattgcac aggaagaacg ctttcttgac
     4201 gttgaacacc aaaatccaca gtcaccctat tatcgcggca gcacggagcc aactcggcca
     4261 cagcggcggc gtttcatggc tggcgccctg gcggctatcg gcgcaaccag ccttccgtca
     4321 g
//
LOCUS       DATCXU010000004         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_865064,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000004 DATCXU010000000
VERSION     DATCXU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865064"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="prs"
                     /locus_tag="VIR04_00080"
     CDS             complement(<1..420)
                     /gene="prs"
                     /locus_tag="VIR04_00080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY9459175.1"
                     /translation="MTHDRFMIFTGTANTRLAVDVVNHLDMSLGKMTVGRFSDGEVMV
                     EINENVRGKDVFVLQPTCAPTNDNLMEIMVMVDALRRASARCITAALPYFGYARQDRR
                     PRSARVSISAKIVANMLQVVGVDRVLTMDLHADQIQGF"
     gene            complement(535..611)
                     /locus_tag="VIR04_00085"
     tRNA            complement(535..611)
                     /locus_tag="VIR04_00085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Gln,seq:ttg)
     gene            complement(613..1527)
                     /gene="ispE"
                     /locus_tag="VIR04_00090"
     CDS             complement(613..1527)
                     /gene="ispE"
                     /locus_tag="VIR04_00090"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY9459176.1"
                     /translation="MGGATLYDVPAPAKINLFLHVVGRRADGYHLLQTAFRFVDLCDT
                     LHFERRGDGKLVCESPLAGLKPQDDLVLRAAQALQRATGTRYGARIQYQKKIPAGGGL
                     GGGSSDAASTLIALNRLWQTRLTRADLMQLALPLGADVPVFIFGQAAFAEGVGELLSP
                     LSLPERSYVVIAPAQTVSTSAVFSSPELTRDAPYVRMLLFTDWQKAQLQAAFFGRNDL
                     EPVVYAKYSRMRALSLWLKHEGVNARMTGSGACFFAEFVTLQEASQCRQAIIAKIPYC
                     EDSKAALIDDVWACRGLHDHPLRHWLHS"
     gene            complement(1532..2113)
                     /gene="lolB"
                     /locus_tag="VIR04_00095"
     CDS             complement(1532..2113)
                     /gene="lolB"
                     /locus_tag="VIR04_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY9459177.1"
                     /translation="MRWRLVKRYRAWWCAACLGLLLAACATPQKVAGGAAGAFDRTGR
                     FSLNVTDNTGQPQAVQGGFAWSDDGRELQLDLANPLGSTLARVEVTPDHATLTRANGT
                     QKQARDPDALVELVLGSPMPVAGLRDWLRGQTGRQSVQGLEKNKAGQPEHFTQNGWKV
                     ELSRYDALGPRLLQLNHSQADRRISVRLVIDSD"
     gene            complement(2113..>3263)
                     /locus_tag="VIR04_00100"
     CDS             complement(2113..>3263)
                     /locus_tag="VIR04_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY9459178.1"
                     /translation="VSEARQALALDPNSEPAAQRLLEYGFKTDPVAAIAATRAFLEKH
                     PEARKLQLMLVGSLVDRDDFNGALTQIRLMRARSPEDFDLLYTEARINFSAGHYAEAE
                     SLLQEYINVQTQRRRSVNDNVTNAVADVADARLLLVQIAEKQNRLDDAIRQLELIDDP
                     SLRFQVRIHQAVLQARQGDLSKARATIAALKPQDKNQRAVAALTLASIYRESGRTDLA
                     AQDLAQADKELPDTPEIKYALAMLYERQGKIDEFEALMRRVIELQPTNADAYNSLGYT
                     MVDQNRRLDEARDLLEHALDMEPDNPFIQDSVGWYFYRVGDNLQALEYLRRAYAQMPS
                     ADVAAHLGEVLWKMKRLDEAKEIWRAGLGKDAKNETLLNTLKRLGIKL"
BASE COUNT          737 a          978 c          877 g          671 t
ORIGIN      
        1 aaagccctga atctggtcgg catgcaaatc catggtaagg acgcgatcga cacccaccac
       61 ttgcagcatg ttggccacga tcttggccga tatcgatacc cgcgccgaac gcggccgccg
      121 gtcctggcgc gcataaccga agtacggcag cgccgccgta atgcaccggg ccgaggcgcg
      181 gcgcaaggcg tcgaccatga ccatgatttc cattaaattg tcgttcgtgg gcgcacaagt
      241 cggttgcagc acgaacacgt ctttgcctcg gacgttctcg ttaatttcga ccatgacttc
      301 gccatcggaa aaacggccca cggtcatctt tccaagagac atgtcaaggt gattgacaac
      361 atccaccgcc aaacgagtgt ttgcggtgcc ggtaaaaatc atgaatctat cgtgtgtcat
      421 gatgggtgat aaggattcca tagcgctaaa caacaaagtt gttgaccagc gcacccggtt
      481 cggggccgac gcaacagccg aagagttaaa gcaaagccgc agggccgtga aaaatggctg
      541 gggaagaagg attcgaacct tcgcatgctg gaatcaaaat ccagtgcctt aaccagcttg
      601 gcgattcccc aattaactgt gcaaccagtg tcgtaacgga tgatcatgca agcccctgca
      661 tgcccacacg tcgtcaatta acgccgcctt gctgtcttca cagtaaggta ttttagcgat
      721 aattgcctgc cggcactggc ttgcttcctg gagcgttaca aattcagcaa aaaagcaagc
      781 ccccgagccc gtcatccgcg cattgacgcc ttcatgctta agccacaagg aaagcgcacg
      841 catgcgggaa tacttcgcgt aaaccacagg ctccagatca ttgcggccaa aaaatgcagc
      901 ctgcagttgg gctttttgcc aatcggtaaa gagcagcatt ctgacgtaag gagcatctct
      961 tgtcaattca ggggaagaaa aaacggcaga tgtggatacc gtttgcgccg gagcaatgac
     1021 aacataggaa cgctcgggaa ggctcagtgg cgagagcaat tcacctaccc cttcggcaaa
     1081 cgccgcctga ccaaaaataa atacgggcac atcggctccc agcggcaatg ccagttgcat
     1141 cagatcggca cgcgtcaacc gggtctgcca caaccggttc aaagcgatca gcgtgctggc
     1201 cgcatcgctg gacccgcccc ccaggccgcc gccggcgggg atcttttttt ggtattgaat
     1261 acgcgcgcca tagcgtgtgc cggtggcgcg ctgtaacgcc tgagccgcgc gcaataccag
     1321 gtcgtcttgt ggtttcagac cggccagggg gctttcacac acaagcttgc cgtcgccgcg
     1381 ccgctcgaaa tgcaaggtat cgcataaatc aacaaaccga aaggcagtct gtaataaatg
     1441 atagccgtca gcgcgccggc caacaacatg cagaaacaga ttgatcttgg cgggtgccgg
     1501 aacgtcgtat aaagtcgcgc cgcccattaa atcagtcgct atcaataacc agacgcacgc
     1561 tgatccgcct gtcggcctgg ctgtgattca gttgcagcaa acgcggaccc agtgcgtcgt
     1621 agcgcgacaa ttccactttc cagccatttt gcgtgaaatg ctcaggttgc cccgccttgt
     1681 tcttttccag tccttgcaca gactgacggc cggtctgtcc gcgcaaccag tcgcgcaaac
     1741 cggcaaccgg catggggcta cccagcacta attcaaccaa agcgtcggga tcgcgcgcct
     1801 gtttttgcgt tccgttggcg cgtgtcaggg ttgcatggtc cggcgtgacc tcgacacggg
     1861 ccagtgtgct acccagcgga ttggccagat caagctgcaa ttcgcgcccg tcatcgctcc
     1921 atgcaaaacc accctgaacc gcctgcggct ggcctgtgtt gtcggttacg ttcaacgaaa
     1981 aacggccggt tctgtcgaac gcgcccgccg cgccgcccgc caccttttgc ggtgtggcgc
     2041 aggccgccag aagcaaaccc agacacgccg cgcaccacca cgcccgatag cgtttgacta
     2101 accgccagcg cattacagct tgatcccgag acgctttaac gtattaagca aagtctcgtt
     2161 ttttgcgtct ttgcccaggc ccgcgcgcca tatttcttta gcttcgtcca gacgcttcat
     2221 cttccaaagc acctcgccca aatgagccgc gacatccgcc gagggcattt gtgcgtaagc
     2281 gcgccgcaga tactccagtg cctgcaaatt atcgcccaca cgataaaaat accaaccgac
     2341 gctgtcctga ataaacggat tgtcgggttc catatcgagg gcgtgctcaa gcaaatcacg
     2401 cgcttcatcc agcctccggt tttgatcaac catggtgtaa ccaagcgagt tataggcatc
     2461 cgcgttggtg ggttgaagct cgatcacccg tcgcataagc gcttcgaact cgtcgatttt
     2521 gccttgccgc tcgtagagca tggccagcgc atacttgatt tcgggcgtat ccggcagctc
     2581 tttgtcggcc tgcgccaaat cctgcgccgc aaggtcggtg cggcccgact cgcggtaaat
     2641 ggacgccagc gtcaaggcag ccaccgcgcg ctgattttta tcttgcggct taagtgcggc
     2701 aatagtggcg cgcgccttgc ttaaatcgcc ctggcgcgcc tggagcaccg cctgatgaat
     2761 ccggacctga aagcgcaacg aagggtcgtc aataagttcc aactgcctga tggcatcgtc
     2821 cagacgattt tgcttctcgg cgatctggac cagcagcaag cgcgcgtcgg cgacgtccgc
     2881 caccgcgttc gtgacgttat cgttcaccga gcggcgacgc tgcgtctgaa cgttaatgta
     2941 ttcctgaagc aaagattccg cttcggcgta atgcccggcg ctgaaattaa ttcgcgcctc
     3001 ggtgtataaa aggtcgaagt cttcgggcga tctggcgcgc attaacctga tctgggtcaa
     3061 tgcgccgttg aaatcgtcac gatccaccag gctgccaacc aacataagct gcaatttgcg
     3121 tgcttcggga tgcttttcca aaaaagcgcg cgttgcggca atcgccgcaa caggatcggt
     3181 cttgaagccg tattcgagaa gccgttgcgc cgccggctcg gagttgggat caagcgccaa
     3241 agcctgtcgc gcctcggata cgg
//
LOCUS       DATCXU010000005         7712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_75526,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000005 DATCXU010000000
VERSION     DATCXU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75526"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2956)
                     /gene="dnaE"
                     /locus_tag="VIR04_00105"
     CDS             complement(<1..2956)
                     /gene="dnaE"
                     /locus_tag="VIR04_00105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY9459179.1"
                     /translation="MTEIGSAPAFVHLRVHSEFSVVDGIVRIPDLIQRVAEFGQPAVA
                     LTDLGNVFGLLKLYKTARKAGVKPVAGCDVWLQNDQDRDKPYRLLVIVRDRLGYSNLC
                     ELLTKAWLDNPYRGRAEIRREWLTGKGGLIIISGARAGDVGQALESGRLAEAKSLAQG
                     WATDFPGSYFIELQRAGFEGDETYVQAAMRLAAEAGLPVVATHPVQFIDAADFRAHEA
                     RVCIAEGEQLANPQRARRFTEDQYLLSSQEMARRFADVPSALANTVEIAKRCNLTIEL
                     GRPRLPNFPTPEGVTLDDYMVHLAEEGLVARMAQLFPDEDERRQKYPQYLERLRWECK
                     TIVSMGFSGYFLIVADFINWGKHNGVPVGPGRGSGAGSLVAYSLRITDLDPIRYVLLF
                     ERFLNPERVSMPDFDVDFCQDNRERVIEYVKQKYGRDAVSQIVTFGTLGAKAVVRDVG
                     RVLEMPYTLCDGLSKMIPFNPVDPWTLDRTLENEPAFKERYEQDEEVRALIDLARPLE
                     GLTRNLGMHAGGVLIAQGKLTDYCPLYCQPGSSTSAVSQFDKDDVEAVGLVKFDFLGL
                     RNLTILDLAVRYVRQFNESERDFDLMALPLDDPATYKLLIDGNTTAVFQLESRGMKEL
                     LKSLRPDTFEDIIALLALYRPGPLESGMVVDFVNRKHGRAKVDYFHPDLEPVLKGTYG
                     VIVYQEQVMLISQIIGGYTLGGADLLRRAMGKKKASEMAKHRAIFEKGAVERGYDAGL
                     AVKLFDLMEKFAGYGFNKSHSAAYALITYQTAWLKAYHPAEFLAATMSCDMDDTDKLQ
                     IFWKDALSNDVVVLPPDINASTYRFEPVNDSYTERGEPPRTMRYGLGAVKGTGQGAVE
                     EIIRAREQGGAFASLFDFCHRVDRHTVNRRSIEALIRAGAFDQIDNNRAALLATVGSA
                     IEAADQAERSANQVSLFADNANDIVEGELAKVAAWDLQTRLMAEKSAMGFYFNGHLFD
                     AWRDEVRRFAPTP"
     gene            complement(3000..6812)
                     /gene="hrpA"
                     /locus_tag="VIR04_00110"
     CDS             complement(3000..6812)
                     /gene="hrpA"
                     /locus_tag="VIR04_00110"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY9459180.1"
                     /translation="MSVKPRVDSSSGPGFARRPPPVEYPEDLPVSGRRKDIAAAIQAH
                     QVVIVCGETGSGKTTQIPKICLEMGRGLERMIGHTQPRRLAATSVARRIAEELRTELG
                     DWVGYQIRFSDRTGPKTAIKLMTDGILLAESQRDPLLRRYDTIIIDEAHERSLNIDFL
                     LGFLKQLLPRRRDLKVVITSATIDAERFAQHFSQNGVRAPVIEVSGRLYPVDIIYRPV
                     AQARDASDVSAGAPRSSGDEERDLVDAVVDGVAECARHGTGDVLVFLPGEREIREVAE
                     ALRKDHPPDVSILPLYARLSQAEQERIFKPKGNARRIVLATNVAETSLTVPGIRYVVD
                     SGLARVKRYSWRNKVEQLHVEPISQASANQRAGRCGRMGPGICVRLYDEADYSARAPF
                     TDPEVLRSSLASVILRMKALRLSDIGAFPFVDAPSGRAIADGYHLLQELGALDDANQL
                     TETGAALARLPVDPRLARMILASNDEQCLAEMLIIASALSVRDPRDRPMSEREASDAA
                     HAKFGDDRSEFLSYLKLWRWYGDQVTHKASQSKLVALLRKNFLSPMRLREWRDVHTQL
                     AALAGEQGWRVNTLDATYEQTHKALLAGLLGNIGLKSEESGGYQGARDIRFQIHPGSH
                     LAKKAGRWIVAAELVDTTRLYARCIAKIDPIWLERVGAHLIHRTWSDPRWEKKAGQVV
                     ANERATLYGLPVYTGRRIHFGKVDPIQARELFIRQALVTGEIDTHLPFVKHNRQLVAE
                     IERLEHQSRRPDILVDDSLIYAFYDQQVPPEICQTVALEKWAKHLDKAAVQALFLTRE
                     ALMRHEAAGITTEVFPKILRWQGVQMSLDYYFEPGSVRDGVTLSVPLLALNQIDPSRC
                     EWLVPGMLKEKVHLLLKSLPQKIRRHCVPLPEYAAGFYERWFDRAADPQMGLLEAISA
                     DMQDQLKIRPLLTDFKVETLPAHLFMNFKVLDEHGRMLSAGRNLAQLKAEHGRQAQAS
                     FQHFAAVDGQVAQALSHERLTDWTFGPLPEVMEIKRKGLSFVGYPALIDRITHCDLDV
                     FDDPHEAQRQHRAGLLRLFRLVLREQVKFFEKNLADLTRMSMLYISLGTREDLKDQII
                     DCAIEQACMSEPWPANKLEFDARRDQGKARFGLLAQEISRLAGQILTDWAALQKKLPQ
                     ARAHPSSYADMQKQLSVLMSKWFIRITPYRQLSHYPRYLRAALARIDKLRADPVRDAR
                     LMADMAPLLAQHQRAFLALKGSPDARLEEFRWLLEELRVALFAQELRTPMPVSVKRLQ
                     KAWSALQR"
     gene            6911..>7712
                     /gene="argA"
                     /locus_tag="VIR04_00115"
     CDS             6911..>7712
                     /gene="argA"
                     /locus_tag="VIR04_00115"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY9459181.1"
                     /translation="MVTDDIETLSAQDAPEFAPAQFVRWFREVAPYVHDFRGKTFIVA
                     FGGELVSDGALNALVQDLSLLSALGVRLVLVHGSRPQVNEQLRLKGYQNQFGRSTEPT
                     SAEALECAKEAAGEIRLDIEAAFSQGLPNTPMSHAQIRVISGNFVTARPVGVIDGIDY
                     KHSGAVRKLDLDAIRNIINQSAIVLLSPLGFSPTGEAFNLAMEDLATSAAVALRAEKL
                     IFLTQDRTVRNPDGTIDTELAREDADALIATGTLDEDTASFLTQASLAV"
BASE COUNT         1741 a         2321 c         2057 g         1593 t
ORIGIN      
        1 gcggcgtggg cgcaaagcgc cggacctcgt cgcgccaggc gtcgaaaaga tgcccattaa
       61 aataaaaacc catggcgctc ttttccgcca taagccgcgt ttgcagatcc caggcggcga
      121 ccttggccag ctcgccctcg acgatatcgt tggcattatc ggcaaacaga gatacctgat
      181 tcgcgctgcg ctcggcctga tcggccgcct cgatagcact tcctaccgtg gcaagcaatg
      241 ccgcgcgatt gttgtctatc tgatcaaatg cgccagcgcg aatcaaggcc tcgatagagc
      301 gccgattaac ggtatgccga tcgacgcgat gacagaaatc aaacagactg gcaaacgccc
      361 cgccctgctc gcgcgcgcga ataatttcct cgacggcgcc ctgccccgtg cctttgactg
      421 cgccaaggcc atatcgcatt gtgcgaggcg gctcgccccg ttccgtatag ctatcgttca
      481 caggctcgaa ccgataggtc gacgcgttga tgtcgggcgg caaaaccacc acatcgttag
      541 acagtgcatc tttccaaaaa atctgcagtt tatcggtatc gtccatatcg caggacatgg
      601 tcgcagccaa aaattcggcc gggtgataag cctttaacca cgccgtctga taggtaatca
      661 gcgcgtaggc ggcggagtga cttttattga agccatagcc cgcgaatttt tccattagat
      721 cgaacagctt gacagccagg ccggcatcgt agcccctctc gaccgctccc ttttcaaaaa
      781 tggcgcggtg cttggccatt tccgaagctt tcttcttgcc catggcgcga cgcagaagat
      841 cggcgccgcc cagggtatag ccaccgataa tctgcgaaat gagcatgacc tgctcctggt
      901 acacaatcac gccataggtg cctttgagca caggttcgag gtcgggatga aaataatcca
      961 ctttggcacg gccgtgcttg cgattgacaa aatcaaccac catcccggat tccagggggc
     1021 caggccggta aagagcgagc aaggcgataa tgtcttcgaa ggtatcgggc cgcaggctct
     1081 tcaaaagttc tttcatgccg cgcgattcaa gctggaacac cgccgtcgtg ttgccgtcaa
     1141 tcaacaactt gtatgtcgcg ggatcatcca gcggcaaagc cataaggtcg aaatcgcgtt
     1201 cgctttcgtt gaactggcgc acgtaacgca ctgccaaatc cagaatcgta agattgcgca
     1261 agcctagaaa gtcgaattta accaggccca cggcctcgac atcgtccttg tcgaactggg
     1321 atacggcact ggtgctggat ccaggttgac aatacaaggg gcaataatca gtcaacttgc
     1381 cttgcgcaat aagaacacca cccgcgtgca tgcccagatt tcgcgtcaac ccctcaagtg
     1441 gtctggcgag gtctatcagc gcgcgcacct cttcgtcctg ttcatagcgc tctttaaacg
     1501 ccggctcatt ttccagtgtg cggtcgagcg tccatgggtc gacggggtta aacggaatca
     1561 ttttcgacag gccgtcgcac agcgtatagg gcatttccag cacccgcccc acgtcgcgca
     1621 ccaccgcctt ggcgcccagc gtgccgaaag tgacaatttg gctgactgcg tcgcggccgt
     1681 atttttgctt gacgtactcg atcacgcgct cgcgattgtc ctggcagaaa tcgacgtcga
     1741 agtccggcat ggaaacccgt tccggattca ggaaccgttc gaacagcaaa acgtatcgta
     1801 taggatcgag atctgtgatt cgcaacgagt acgccaccag cgagcctgcc cccgagccgc
     1861 gcccaggccc caccggcacc ccattgtgtt tgccccagtt aatgaagtcg gccacgatca
     1921 ggaaatagcc tgaaaagccc atgctgacaa tcgttttgca ttcccaacgc aatcgctcaa
     1981 gatattgcgg gtatttttgc cgcctttcat cctcgtccgg aaacaattgg gccatacgcg
     2041 ccacaaggcc ttcttcggcc agatgaacca tgtaatcgtc gagcgtcacg ccttcgggcg
     2101 tggggaaatt tggcagacgc ggcctgccca gctctatcgt caaattgcag cgcttggcaa
     2161 tttcgaccgt attcgccagg gcggagggca cgtcggcaaa acgccgcgcc atctcttgcg
     2221 aacttaataa atattgatcc tctgtgaagc ggcgcgcacg ttgcggattg gccaattgct
     2281 cgccctcggc aatgcacaca cgagcttcgt gagcgcgaaa atcagccgcg tcgataaact
     2341 gcacaggatg tgtcgcaacc accggcaaac cggcctcggc cgccaaacgc atggccgcct
     2401 gaacataggt ttcgtcgccc tcgaagcccg cgcgttgcag ttcaatgaaa tagctgccgg
     2461 gaaaatccgt agcccagcct tgcgcaaggc tttttgcctc ggcgaggcgg ccggactcca
     2521 gagcctgccc gacatccccg gcgcgggcac ccgaaataat aattagtccc cctttgcctg
     2581 ttagccattc acgccgaatt tcagcccggc cccggtaggg gttgtcaagc cacgcttttg
     2641 tcaataattc gcacagattc gaatagccca accgatcacg cacgatgaca agcaagcgat
     2701 agggtttgtc ccgatcctga tcattttgca gccagacatc gcagccggcg acgggcttga
     2761 cccccgcctt gcgcgccgtc ttgtaaagtt taagcaggcc gaaaacattg cccagatcgg
     2821 tcagtgccac ggctggttgc ccgaattcag ctactcgttg aattagatca ggaatccgca
     2881 caatgccgtc caccaccgaa aactcggagt gaacacgcaa gtgcacaaaa gcaggggcag
     2941 agcctatttc agtcataatc aggattgtac ccagacgagc gttgttctag ttctgattgc
     3001 tatcgctgca aggcagacca tgccttctgc aagcgcttca cagaaaccgg catgggcgtt
     3061 cggagttctt gcgcaaacag ggccactctc agctcctcca atagccagcg aaactcctcc
     3121 agacgcgcgt ctggcgaccc tttaagcgcc aaaaaggcgc gctggtgctg ggccagcaac
     3181 ggcgccatat ccgccatcag gcgggcatcg cgaacaggat cggcgcgcaa cttgtcaatc
     3241 cgggccaacg ccgcccgcag gtaacgaggg taatgcgaca attgccggta tggggtaatt
     3301 cgtatgaacc atttactcat taaaacgctc aattgctttt gcatatcggc gtaactggac
     3361 gggtgtgccc tggcctgagg cagcttcttt tgcaatgccg cccagtccgt caaaatctga
     3421 ccagccaggc gggaaatttc ctgagccaat aacccgaatc gagcctttcc ctgatcgcgg
     3481 cgagcgtcaa attcaagctt gttagccggc cagggctcac tcatgcatgc ctgctcaatc
     3541 gcgcagtcga taatctggtc tttgagatcc tcccgagtcc cgaggctgat atacaacatg
     3601 ctcatgcgag tcagatcggc cagatttttt tcaaaaaact tcacctgttc acgtaaaacc
     3661 agacgaaata aacgcaaaag gccagcacga tgctggcgct gcgcctcgtg cggatcgtca
     3721 aaaacgtcca ggtcgcaatg agtaatgcga tcgatcagcg ccggataccc cacaaacgac
     3781 aaacccttgc gtttgatttc cataacttcc ggcaaggggc cgaaggtcca gtccgtcaaa
     3841 cgttcatggg acagcgcctg cgccacctgg ccgtcgaccg cggcaaaatg ctgaaaagac
     3901 gcctgcgcct gacggccgtg ctcggcttta agctgggcca ggtttcgtcc ggccgacagc
     3961 atgcgcccgt gctcatcaag cactttgaag ttcatgaaca aatgggccgg cagcgtttcc
     4021 actttgaagt cagtgagcaa aggcctgatt ttcaactgat cctgcatgtc ggctgaaata
     4081 gcctcgagca agcccatctg gggatcggcg gcccggtcaa accagcgttc gtaaaagccc
     4141 gctgcatact caggcaaagg cacgcaatga cggcgtattt tttgcggcaa cgattttaaa
     4201 agtaaatgca ccttttcctt caacatgcca ggcacgagcc attcacaacg agaaggatca
     4261 atttgattta gcgccagcaa gggcacgctt aatgtgacgc catcgcgcac cgagccaggc
     4321 tcgaaataat aatcgagcga catctgcacg ccctgccacc ggaggatttt tggaaagact
     4381 tccgtcgtga tgcccgccgc ctcatggcgc atgagtgcct cgcgggtcag gaacagagcc
     4441 tgaacggcgg ctttgtccag atgtttcgcc catttctcca gcgccacagt ctggcagatt
     4501 tcgggcggca cttgctgatc gtagaatgcg tagatcaaag aatcatcgac cagaatatcg
     4561 ggccggcgcg attgatgctc cagccgttca atttccgcga cgagctgacg attatgtttt
     4621 acaaaaggca aatgcgtgtc gatttcgccc gtgaccagcg cttgccggat aaaaagctca
     4681 cgagcctgga tggggtcgac cttgccaaaa tgaattcgac gcccggtgta aaccggcaag
     4741 ccatacaggg tggcacgttc gttggccaca acctgacctg cttttttctc ccagcgcggg
     4801 tccgaccacg tgcgatgaat cagatgcgct cccactcttt caagccatat cggatcaatc
     4861 tttgcaatgc aacgggcgta cagccgcgtt gtgtcgacca gttcagccgc aacgatccag
     4921 cgccccgctt tcttcgccag atgcgatccc ggatggatct gaaagcggat atcgcgcgcg
     4981 ccctgatagc cgcctgattc ttcgcttttc aagcctatgt tgccaagcag gccggcgagc
     5041 aaggctttat gggtttgctc ataggtggcg tccagcgtgt taacgcgcca cccctgttcg
     5101 ccagccaaag ccgccaattg ggtgtgcaca tcgcgccatt cccgcaagcg catgggtgaa
     5161 agaaagttct tgcgcaacaa ggcgaccagc ttgctctggg aagccttgtg agtcacttga
     5221 tcgccatacc agcgccacaa cttcagatag gataaaaatt cggatcggtc atcgccaaac
     5281 ttcgcgtgcg ccgcgtcgga cgcctcacgt tcgctcatgg gccggtcgcg cggatcgcgc
     5341 acggacaagg cggaagcaat aatgagcatc tccgccaggc attgctcgtc gttgctggcc
     5401 aggatcatgc gcgccaggcg cggatccacc ggcagacgcg caagcgcggc gcctgtttca
     5461 gtcaattgat tggcatcgtc tagcgcaccc aattcctgca aaaggtgata tccgtcggct
     5521 atggcgcgcc ccgacggcgc atcgacaaac ggaaaagctc caatgtcgga cagccgcagc
     5581 gccttcatac gcaaaatgac agaggccagc gatgagcgca gcacctcggg atcggtaaac
     5641 ggcgcgcggg cgctgtaatc ggcctcatca tacaaacgaa cgcaaatgcc cggccccatt
     5701 cggccgcaac gccctgcgcg ctgattggcc gacgcctggc tgatgggctc aacgtgcagt
     5761 tgctccactt tattgcgcca ggaataccgc ttgacccgcg ccagtccgct atcgaccaca
     5821 tagcggatgc ccggcacagt cagcgatgtt tcagccacat tggtggccaa tacgattcgg
     5881 cgtgcgttgc ctttcggttt aaaaattcgt tcctgctcag cttgcgacaa gcgcgcataa
     5941 agcggcaata tgctcacatc aggcggatga tctttgcgca aagcttcggc gacttcgcga
     6001 atttcgcgct cgcccggcaa aaagaccagt acgtcgcccg tgccgtggcg cgcgcattca
     6061 gccacgccgt caaccactgc atcaaccaga tcgcgctctt cgtcgccgct ggaacgcggc
     6121 gcgccggctg acacatcgga cgcatcgcgc gcttgcgcca cggggcgata aataatgtcg
     6181 accgggtaca agcgccccga tacctcgatg accggcgcgc ggacgccatt ttgcgaaaaa
     6241 tgctgggcga aacgctcggc gtcgatcgtt gccgatgtga tgacaacttt caaatcacga
     6301 cgcctgggca ggagctgctt tagaaagccg agcaagaagt cgatgttcaa actgcgttcg
     6361 tgggcttcat cgataataat tgtgtcgtaa cgccgcaaca acggatcacg ctgagactcg
     6421 gccagcaaaa tgccgtccgt catcagcttg atggctgttt tcgggccggt gcgatcgcta
     6481 aaccgaatct gatacccgac ccagtcgccc agctcggtcc taagttcttc cgcgatgcgc
     6541 cgcgccaccg atgtggccgc caggcgccgc ggttgcgtgt gaccaatcat cctttccaga
     6601 ccgcggccca tttcaaggca gattttcggg atctgagttg tttttcccga acccgtttca
     6661 ccacaaacga tcacaacctg atgcgcctgg atcgccgcgg cgatgtcttt tctgcggccg
     6721 ctgaccggca aatcttcggg gtattcgaca ggaggcgggc gccgggcgaa gcccgggccg
     6781 gaagaggaat ctacgcgtgg ttttacagac aaattaacgg tctcgtgcaa aaaactgaat
     6841 ttaaaggacg actgcttatt ataatttcgc tcgagggtga attccctatt tcgcaattac
     6901 attaaaagcc atggtcaccg acgacataga aaccctatcc gctcaagatg ctcccgaatt
     6961 cgcgccagcc cagtttgtgc gatggttccg ggaggtagcg ccttacgtgc atgacttccg
     7021 aggcaagaca ttcatcgtcg cgtttggcgg cgaactcgtc agcgacggcg cgttgaatgc
     7081 tcttgtacag gatttgtcgc tgctatcggc cttgggggtc agactggtcc tcgtccatgg
     7141 ctcgcgccct caggtcaatg aacagcttcg attaaaaggc taccagaatc aatttgggcg
     7201 aagcaccgaa cccaccagcg ccgaagcgct ggaatgcgcc aaagaggccg ctggcgaaat
     7261 tcggcttgat atcgaagcgg ctttcagcca gggcctaccc aatacgccca tgtcgcacgc
     7321 ccagattcgc gtcatttcgg gcaatttcgt caccgcccgc cccgtcggcg tcattgatgg
     7381 cattgactat aagcattccg gggcggtgcg caagctggat ctcgacgcca tcagaaacat
     7441 tattaaccaa agcgcgattg tattgctttc gccactgggc ttttcaccca ccggtgaagc
     7501 cttcaatctg gccatggaag atctggccac cagcgccgcc gttgcgctac gcgctgaaaa
     7561 gctgatcttt ctaacgcaag accggacggt gcgcaatccc gacggtacca tcgacacgga
     7621 actggctcgc gaagatgccg acgccttgat cgccacagga acgctggatg aagacaccgc
     7681 ctcgtttttg acccaggctt cgctagccgt ca
//
LOCUS       DATCXU010000006         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1094071,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000006 DATCXU010000000
VERSION     DATCXU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094071"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VIR04_00120"
     CDS             complement(<1..1185)
                     /locus_tag="VIR04_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459182.1"
                     /translation="MSAQSDVQAAAQPTAPAGQDRPVQRRVLVFVTISLALMMMAIDS
                     TIVATALHSLQHELNASVNWAGWTITAYSFGFVLMLPISGKLSGQYGRRKVFLGSVIL
                     FTTASLLCGMAGNIYELVALRALQAAGGAGFTPSATGIVVDYFGKERDRAVSLFGSIF
                     PIGAMIGPIFGGLFVTYWTWRGIFFVNVPIGVAVIVLTLRYIPRDLPKPSGSRTKTDL
                     TGMALLGAGVLAGMLAISYLGEPDASVWSPFFTAPLALALATLWAFIRHIERSKRPYI
                     APHLIFGSGFGVVNLINAIYGGVRSGVVALIPLYATNRYGLDALSSGTLLIAQGVATI
                     VLSITAALMIRRTGYRMPLYVGGTVVAIGVLLLAVSPVAGISPYTWRAGAALLVGAGG
                     GAL"
     gene            complement(1359..2855)
                     /locus_tag="VIR04_00125"
     CDS             complement(1359..2855)
                     /locus_tag="VIR04_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY9459183.1"
                     /translation="MDNPSNAPKANRRHLQQIITGLSEGVMLIDRDRSIAWANETALE
                     MHDANTLRDLGQTATGYRKKYILKYRNNHRLLPRQYPIERVLSEEEFTDVVVDVFRAD
                     DDEFRRTHQVRSIVLSDDAGNSELAVLIMLDVTERFNAENRFEKTFNANPAPALICRL
                     SDLRYVKVNQGFLEMTGYSREEIIGRSTYEIDILEGAEKKDQAIAALSEGKTIPQMES
                     TLKLPKGRIKSVIVAGQPIEDGNEDCMLFTFMDLDARKKAEGALRQMEERFSTAFRLA
                     PVPMTLSTLENFRLLEVNDAFLTTTGYTKEEIMGRDSADLAIWANQAEYQKLEKLLEQ
                     TNSIRSYEIQLRSKEGTLLDCLASAEAVTINDERCVLGVIQDITERKRSEVDLIAAIE
                     TVMQDTSWFSRTVIEKLAQIRQPQGKRGKIGELADLTSRERDVLGLICQGLDDNEIGN
                     TLHLSRHTVRNHVATIYSKIDVHRRSAAVVWARERGIVGFETPSQHKK"
     gene            2931..3281
                     /locus_tag="VIR04_00130"
     CDS             2931..3281
                     /locus_tag="VIR04_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459184.1"
                     /translation="MFVSGLPEGLFNTDARYGMGYSKFIQELNKGIEGQITAYSRKDN
                     EEGCLIYLRSPEQRIFVTADVMNHRRDESIQVLRAKIREGVKSTPVLVLKLRGNDLVF
                     EKDIIVQESRAAEF"
     gene            complement(3247..4713)
                     /locus_tag="VIR04_00135"
     CDS             complement(3247..4713)
                     /locus_tag="VIR04_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY9459185.1"
                     /translation="MTNLNGRIEHIVVLMLENRSFDSMLGALKLEKARLDGLSGNETN
                     PLHGQPDVPVWCSNDLSPESMCIPSPDPGELWDDINMQLFGLDGKPGNMPPPMNGFVD
                     NYTRQTAEPTGDYSPAAVMHYYTENQIPVLSALAKQFAVCDQWFASAPCQTWPNRFFL
                     HTGTASGYENNSPAHFPYLMHTIFNRLTEAGKPWKIYFHDFPQSLTLTSLWPYAGRFR
                     FMGEFAQDAKSGNLPAYSFIEPRYFPDVTLPNDQHPPHNVGMGESLIFDIYNAIRNAP
                     TWKKTLFIITYDEHGGCYDHVPPPRAVPPDASNPGVFGFDRYGVRVPAVLISPYIKPN
                     TILRVPPDGKLGTQGPPYPFDHTSIIATLRKCFDLGAPLTQRDAVAPDLESVLSLDTP
                     DNAGPAQLKPLTYPMTPADIQRAMNAPLSSAQKTMHEVAAHLPPLGAVTGAANVFSTT
                     EAQVGNMVNGVLAATPNHKTPAEALPFIKTRLHDFLGR"
     gene            complement(4736..>6588)
                     /locus_tag="VIR04_00140"
     CDS             complement(4736..>6588)
                     /locus_tag="VIR04_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY9459186.1"
                     /translation="PLRAELTMAITGTPVGVWIGVLAAIIILLGLRVRAGRALMRLTS
                     TRVPPGRLRRSLFAVALVLLAAVVPGLIAELVRTGVNWNALLSESTDALLGRLVGIIG
                     YGGFVAGLGHALLAPERPSWRLPPILDTVAAGLRWFPLSLAAIIVIGWSAERLGSLIN
                     VSLSATVALDCLMALALNTVIGLALARARRLRRDFNNDPDITDHPPIPLWLAVLPGLA
                     WLTLTVSVVCVLTGYVAFGNFVVKQLVWIATVLSSAYLLTTLIHDGCASLQTTIKRNA
                     DDENLTHARTRTQSQALVLFSGLGRLLVVLLALILLLAPFGEGPTELLGHIDYLHAGI
                     AIGEVQIRPAAVLQSVLVLLLSFGAVKVFQRWLEVQYLPTTSLDPGMRISAATLFGYV
                     GYVIAVALALSAAGIGLERVAWIASALSVGIGFGLQAVVQNFVSGLILLAERPVRVGD
                     WVSLGGVEGDIRRINVRATEIQMSDRSTVIVPNSEFITKVVRNVTHANPLGLVQIKLP
                     MPLSTDPEQVRSLVLAAFQDHVEVLDEPMPKVFLDTIDANGLVFNATGYVNSPRAAYT
                     VRSALLFDVLVRLHEANLSLAKPPTMIVASPAQSNPPDLPDTSAAARRAR"
BASE COUNT         1413 a         1791 c         1917 g         1467 t
ORIGIN      
        1 gagtgcgccg ccgccggcgc cgacaagaag cgcggctccc gcccgccaag tatagggcga
       61 gatgccggca accgggctga cggcaaggag cagcacacct atggcaacga cagttcctcc
      121 gacatacagc ggcatgcggt agccggtgcg ccgtatcatc agcgcggccg taatcgacaa
      181 tacgatcgtg gcgactccct gagcaatcag cagcgtgccg gagctcaagg catccagccc
      241 gtagcggttg gttgcataga gggggatgag cgccaccacg ccggaccgga cgccgccgta
      301 tatggcattg atcagattga ctacgccgaa gccggatcca aaaatcaggt gtggcgcgat
      361 ataggggcgc ttcgatcgtt cgatatgacg aataaacgcc catagggttg ccagtgcgag
      421 cgccagcggg gccgtgaaaa atggcgacca tacgctggca tccggttctc caaggtagct
      481 gatcgccagc atccccgcga gcacaccggc gcctagcagc gccatcccgg tcaggtctgt
      541 tttcgtgcgc gaccccgacg gctttggcag atcgcgaggt atgtaccgca gcgtcagcac
      601 gatgaccgcc acaccaatgg gcacgttgac gaaaaaaatg ccccgccaag tccagtaggt
      661 gacgaacaag ccgccgaata tgggtccgat cattgccccg attggaaaaa tgctgccaaa
      721 caggctcacc gcgcgatcgc gctctttgcc gaagtagtca acgacgatac ccgtggccga
      781 gggtgtgaag cccgctccac ccgcggcttg gagagcgcgc aacgccacaa gctcgtagat
      841 gttgccggcc atgccgcaca acagcgaagc ggtggtgaaa agtattaccg agccgaggaa
      901 tactttacgc cgcccatact gcccactgag cttgccgctg atcggcagca tcagtacaaa
      961 tccgaacgag taagctgtga tggtccagcc cgcccagttg accgatgcat tcagctcatg
     1021 ctgcaaagag tgcaatgccg ttgcgacgat ggtggaatcg attgccatca tcatcaatgc
     1081 gagcgagatg gtcacaaaga ccaggacgcg tcgttgtacg ggccggtctt gtccggccgg
     1141 cgccgtaggc tgggccgccg cttgaacatc agattgggcg ctcacgttac gcgtttttca
     1201 ttaaggcaat ggtcaccgcc agcgattgaa cggcatggga tcaaagggtt tcttctcgag
     1261 gcgatagtac ttccgataaa cgctgcccga caagctggcg atctatttac aatcgttatg
     1321 tctttaactg cgtagatagc ggcgttgtgc gtccggcatc attttttatg ttgtgacggc
     1381 gtttcaaagc cgacgatgcc tcgctcccgt gcccacacca cagcggcgct gcgcctgtgg
     1441 acatcaattt tgctgtagat tgtcgcaaca tgatttcgca ctgtgtggcg cgacagatgc
     1501 agggtgtttc ctatttcgtt gtcgtccagt ccctggcata taagaccgag aacgtcacgt
     1561 tcgcgcgatg tcaaatccgc cagttcgcca attttgccgc gcttgccttg cggctgacgt
     1621 atctgagcca gtttttcaat gaccgttcgg ctgaaccacg atgtgtcctg catgacggtc
     1681 tcgatcgcgg caatcagatc gacctcggat cgcttgcgct cggtaatatc ctgaatcacg
     1741 ccgagcacac agcgttcgtc gtttatagtg acggcctcgg ctgatgccag acaatccagc
     1801 aatgttcctt ctttgctgcg gagctgaatc tcgtaactgc gaatactgtt ggtttgttcc
     1861 agcagtttct ccagcttctg atattcagcc tgattggccc atatggccag atcggccgaa
     1921 tcgcggccca tgatctcttc cttggtgtag cccgtcgttg tcaggaatgc gtcattgact
     1981 tccagcaagc ggaaattctc aagcgtgctg agcgtcatcg gtactggcgc cagccggaat
     2041 gctgtggaga aacgttcctc catttgccga agcgcgcctt cggctttttt gcgcgcgtca
     2101 agatccatga acgtgaagag catgcaatct tcatttccgt cttcgatagg ctggccggcg
     2161 acgatgacag actttatccg tcccttgggc agtttaagcg ttgactccat ttgtggaatt
     2221 gttttgcctt cgctcagggc ggcaattgcc tgatcctttt tttcggcgcc ttccaaaata
     2281 tctatttcgt aagtggagcg gcctatgatt tcttcccgag agtatccggt catttccaga
     2341 aagccctggt ttacttttac gtaccgtaag tcggacaggc ggcatatcaa ggcgggcgca
     2401 ggattggcgt tgaatgtttt ttcgaagcga ttttccgcat tgaagcgttc agtgacatcc
     2461 agcattatca gtacggctag ttcagaattt cccgcgtcgt cgctcaggac tatgctgcga
     2521 acttgatgtg tgcgccgaaa ctcatcatcg tctgcgcgaa agacatcgac tactacgtcg
     2581 gtgaactctt cttccgaaag tacgcgttct atcgggtatt gtctgggtag aaggcgatgg
     2641 ttgttgcggt acttgagtat gtattttttt cggtagccgg ttgctgtttg gccaaggtct
     2701 cgaagcgtgt ttgcatcatg catttccagg gctgtttcgt tggcccaggc gatggagcga
     2761 tcacgatcaa tcagcataac cccttcgctc aaaccggtaa ttatttgctg gagatgccgg
     2821 cggttcgctt tgggcgcatt ggaggggtta tccatttatg gctcctgcta aaagtactag
     2881 atagattgat taaacaaaat ctgtaccgag ggcggacgga catttaagct gtgttcgtct
     2941 ccgggcttcc ggagggctta ttcaataccg acgcgagata tggcatgggc tacagcaaat
     3001 ttattcaaga gcttaacaag ggcatcgagg gccagattac ggcatattcc agaaaggaca
     3061 atgaagaagg atgtctgatt tatttgcgga gtccggagca aaggattttc gttaccgcgg
     3121 acgtgatgaa ccatcggcgt gatgaatcga ttcaggtatt gcgcgccaag atccgcgaag
     3181 gtgtgaaatc gactcctgtc cttgtcctga aattgcgtgg aaacgacctc gtatttgaaa
     3241 aggacattat cgtccaagaa agtcgtgcag ccgagttttg atgaacggca acgcttcggc
     3301 cggagtcttg tggtttggcg ttgcggcaag cacgccattt accatgttgc caacttgcgc
     3361 ttcggtggtg ctgaaaacat tggcggcgcc tgtaactgcg cccagcggcg gcaggtgcgc
     3421 cgcaacctcg tgcattgttt tttgcgcgct gctaagcggt gcgttcatcg ctcgttgaat
     3481 atccgccggc gtcatcgggt atgtcaaagg tttgagttgc gcaggccccg cattgtcggg
     3541 ggtatccagg cttaaaacac tttccagatc gggtgcgacg gcatcacgct gagtcagcgg
     3601 cgcgcccagg tcgaagcatt ttcgcagcgt tgcaatgatc gatgtgtggt caaacggata
     3661 gggcggtcct tgtgttccca gtttgccatc gggcggcacg cgcaagatgg tgtttggctt
     3721 gatgtagggc gaaatcagca cggccggaac gcgcacgccg tatcgatcga acccgaaaac
     3781 ccccgggttc gacgcgtccg gaggaacggc tcgtggtggc ggaacgtgat cgtaacagcc
     3841 gccatgctcg tcgtacgtaa taatgaagag agtcttcttc catgtgggcg cgtttcggat
     3901 tgcattgtag atgtcgaaaa tcaggctttc gcccattccg acattgtgag gcgggtgctg
     3961 gtcgttcggc agtgttacgt ctggaaaata gcgtggctca ataaaggaat aggcaggcaa
     4021 gtttccgctt ttggcgtctt gcgcaaattc gcccatgaac cggaaccggc ccgcatacgg
     4081 ccataaactg gtcagggtaa gcgattgagg aaaatcgtgg aagtagattt tccatggctt
     4141 tccggcttcg gttagccgat tgaagatcgt atgcatcagg taggggaaat gggcgggcga
     4201 gttgttctcg tatcccgatg cggtccccgt atgcaaaaag aagcggttcg gccacgtttg
     4261 gcatggtgct gacgcaaacc attgatcgca gacggcaaat tgttttgcca gggcgctaag
     4321 gaccggaatt tgattttcgg tgtagtagtg catgacggcg gcgggcgaat aatcccccgt
     4381 gggctccgcc gtttgtcgag tgtagttgtc gacgaaaccg ttcatcggtg gcggcatgtt
     4441 acccggtttg ccatccaggc cgaatagctg catgttgatg tcgtcccata attcgcctgg
     4501 gtcgggcgat ggtatgcaca ttgactcggg gctgagatcg ttgctgcacc ataccgggac
     4561 gtcgggttgg ccatgcagtg ggttagtctc gtttcccgaa agcccgtcaa ggcgtgcttt
     4621 ttccaatttc aaggcgccca gcatgctgtc aaacgaacga ttctccagca tcagaacgac
     4681 gatgtgctct atccttccgt tcaggttcgt cattgaatag ttcctttgtc aatttttatc
     4741 gcgctcttcg cgctgcggcg ctggtgtctg gaagatcggg tggattcgac tgggctggcg
     4801 aggccacgat catcgtaggc ggcttggcca gcgacagatt tgcctcatga aggcgcacaa
     4861 gcacgtcgaa cagcaaggcg ctgcgcactg tataagccgc acgtggcgaa ttcacatagc
     4921 cggttgcatt aaataccagg ccattcgcat cgatggtgtc caggaacacc ttgggcatgg
     4981 gttcatcgag cacttcgacg tggtcctgga atgccgcaag aacgagactg cgcacctgtt
     5041 cggggtcggt cgacagcggc atcggcaatt tgatctgcac cagtccaaga gggttggcgt
     5101 gcgtgacatt gcgcaccacc ttggtgataa attccgagtt gggcacgatg acggtggagc
     5161 ggtcgctcat ttgaatctct gttgcgcgca cattaatgcg gcgtatatcg ccttcgactc
     5221 cgcccagcga tacccagtcg cctaccctga cgggacgttc cgccagaagg atcaggcccg
     5281 acacgaaatt ttgcactacg gcctggagcc cgaaaccaat acccaccgac agcgcgctgg
     5341 cgatccaggc aactcgttcc aacccgatgc ccgccgccga cagagcaagt gccactgcga
     5401 tcacgtagcc gacatagcca aatagcgtgg cggccgatat tcgcatgcct ggatccaggc
     5461 tggtggtggg caggtactgg acttcaagcc agcgttgaaa tacttttacg gctccaaaac
     5521 tcaataacaa caccagcacc gactggagta cggcggcagg gcgaatttga acttcgccaa
     5581 tggcaattcc ggcgtgcaga taatcgatgt ggccgagcaa ttcagtgggg ccttcgccaa
     5641 atggcgccag tagcagtatt agtgcgagca ataccaccag cagccggccc aggccggaaa
     5701 acaataccag cgcctggctt tgcgttcgcg tgcgcgcgtg cgtcaggttt tcgtcatcgg
     5761 cgttgcgttt tattgtggtt tgcaggctcg cgcagccatc gtgaataagc gtggtcagca
     5821 aataggccga actgagcact gtggcgatcc agacgagctg tttgacgaca aagttgccga
     5881 acgcaacgta gcccgtcagc acgcagacga cgctgaccgt cagcgtcagc catgccaggc
     5941 ccggcaatac ggcaagccaa agcggaatgg gcggatggtc ggttatgtcc gggtcgttat
     6001 tgaagtcccg ccgcaagcgc ctggcgcggg ccagcgccag tcctatgacg gtgtttagcg
     6061 ccagcgccat aaggcagtcc agcgcgaccg tcgcggatag actgacattg atcaacgagc
     6121 ccaaccgttc ggcagaccac ccaatgacaa ttattgccgc cagcgacagc gggaaccagc
     6181 gtagccccgc ggctactgtg tcgagtatgg gaggcaggcg ccatgaggga cgttcgggcg
     6241 ctaatagcgc gtgacccagg cccgccacga aaccgccata accgataata ccaactagcc
     6301 ggccgagtag cgcgtcggtg gattcagata acagagcgtt ccagttgacg ccggtgcgca
     6361 ccagctccgc aatcagtccg ggtacgacgg cggccaacag aacaagcgcc acggcaaaca
     6421 aagagcgccg cagtctgccc ggcggtacac gcgtgctggt tagccgcatc agggcgcgtc
     6481 ctgctcgtac gcgcaacccc agcaagatga taatggccgc tagcacgcca atccacacac
     6541 cgactggggt gcctgttatg gccattgtca gttccgcccg cagcggct
//
LOCUS       DATCXU010000007         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_784704,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000007 DATCXU010000000
VERSION     DATCXU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784704"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VIR04_00145"
     CDS             <1..679
                     /locus_tag="VIR04_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY9459187.1"
                     /translation="SHILFPATAAGKNVAPRVAARLDVAQVSDVIAVDAPDTFQHPIY
                     AGNAIATVQSADAIKVVTVRTTAFDAAPGQGGAASVEVLTAQADSGLSSFVGREVAKS
                     DRPELAGAKIVVSGGRGMGSAENFKLLNPLADKLGAALGASRAAVDAGYAPNDWQVGQ
                     TGKIVAPQLYMAIGISGAIQHLAGMKDSKVIVAINKDAEAPIFGVADYGLVADLFEAV
                     PELVKAL"
     gene            822..>2423
                     /locus_tag="VIR04_00150"
     CDS             822..>2423
                     /locus_tag="VIR04_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9459188.1"
                     /translation="MNYRAPTQEILFVLKELADLDGVLALPGLEEITPDLVQAVLEEN
                     ARFVEQAVAPLNHSADASPAVWLDGKVTTSPGFRKAFREFAQGGWQGLQHDPKWGGQG
                     LPKLVGAPSSENINAANLAFSLCPLLTDGVIEALATVGSADQQSRFIPPMLQGRWTGT
                     MNLTEPQAGSDLALVATRAVPQDDGTYRLSGQKIFITYGEHDLAENIVHLVLARTPNA
                     PAGVKGISLFIVPKFLVNDDGSLGARNDVWCASIEHKLGIHASPTAVMLYGSDKGAVG
                     AGAIGQLVGEENRGLEYMFIMMNAARYAVGVQGIALAERALQQAQAYAHERLQGRAIE
                     GSREAVAIVRHPDVQRMLMTMRALTEGARAVAYVAAAACDKSRRHADPNVREQNRAIY
                     EYLVPVVKGFSTESAVEVASLGIQVHGGVGFIEETGAAQYYRDARILPIYEGTTAIQA
                     NDFVGRKTLRDGGMVARAVIDQMRATVVELEARAATSGAHRAGLTLVARHLDAAVVAY
                     EKALEYVLANAAESLRAVFLGSVPYL"
BASE COUNT          458 a          655 c          807 g          503 t
ORIGIN      
        1 cagccatatt ttgtttcctg cgaccgcggc gggcaagaac gtggcgccgc gcgtggcggc
       61 gcggctggat gttgcgcagg tttccgatgt aatcgcggta gacgcgcccg acactttcca
      121 gcacccgatc tacgcgggca atgccattgc cacagtgcaa tcggctgatg cgatcaaggt
      181 cgttaccgtg cgcactacgg cgttcgacgc cgcgccgggc cagggtggcg cggccagtgt
      241 tgaagttctg acggctcaag ccgattcggg cctgtcttcg ttcgtgggcc gcgaggtggc
      301 caaaagcgat cgtccggagc tggctggcgc caagatcgtg gtgtctggtg gacgcggaat
      361 gggcagcgcc gagaatttca agcttctcaa tccgttggcg gataagctag gcgcagcgct
      421 gggcgcatcg cgcgctgcgg tcgatgccgg ttatgcgcct aacgattggc aggtggggca
      481 gacgggaaag attgtcgctc cgcagttgta catggccatc gggatttctg gggctattca
      541 gcatttggcc ggcatgaagg attcgaaagt tattgttgcc atcaataaag atgcggaagc
      601 gccgattttc ggagtggccg attatgggct ggtggctgat ttgtttgagg ctgtgcctga
      661 gttggtcaag gctttataga tacgtctttg ccactgaggg cttggcagct ttgtcccatg
      721 cagcggcgat agcgccggta ttgtcccaag cgtcaatggg cgcagtcgcc gaatgtatgc
      781 gaatacaatc gaaagtcgaa tggtcgttac tgggagaaga cgtgaactat cgggcgccta
      841 cacaggaaat ccttttcgtg ctcaaggagc tggccgacct tgatggtgtt ctggccttgc
      901 cgggccttga agaaatcacg ccggatctgg ttcaggccgt gctggaggaa aatgcccgct
      961 tcgtcgagca agccgtcgcg ccgctgaatc acagtgccga cgcgtcgcct gccgtgtggc
     1021 tggacggcaa ggtgaccacc tccccgggtt ttcgaaaggc atttcgcgag tttgcgcaag
     1081 gcggctggca gggcttgcaa catgatccca aatggggcgg acagggtctg cccaagctgg
     1141 tgggtgcgcc gtccagcgaa aatataaacg cggcaaatct ggctttttct ctatgtcccc
     1201 tgttaaccga tggcgtgatc gaggcgctgg ccacggtggg aagcgcagat cagcaatcgc
     1261 gctttatacc ccccatgctc cagggtcgct ggacgggaac aatgaatttg accgagccgc
     1321 aagcgggctc tgatttggcc ctggttgcga ccagggcggt tccgcaggac gatggcacgt
     1381 atcgcttgtc gggccagaaa atattcatca cttatggcga acacgacctg gcggaaaaca
     1441 ttgtgcacct ggtgttggcg cgcacgccca acgcgccggc gggagtgaag ggcatttcac
     1501 tgttcattgt gccaaagttt cttgtcaatg acgacggctc gctgggcgcg cgcaacgacg
     1561 tctggtgcgc ttctattgaa cacaaactgg gcatccacgc cagtccgacg gcagtgatgc
     1621 tctacgggtc ggacaagggg gcggtcggcg cgggagcaat cggccagttg gtgggggaag
     1681 aaaatcgcgg cctggaatat atgttcatca tgatgaacgc ggcgcgctat gccgttgggg
     1741 tgcaggggat tgcgctggcc gagcgcgcac tgcaacaggc gcaggcctat gcgcatgaac
     1801 gtcttcaggg ccgcgcgatt gaaggctctc gggaagcggt ggccatcgtc cgccatcccg
     1861 acgtacagcg catgttgatg accatgcggg cgctaaccga aggcgcgcgt gcggtggcct
     1921 atgtcgcggc agccgcctgt gacaaatccc ggcgtcatgc cgaccccaac gttcgtgagc
     1981 agaatcgggc gatctacgag tacctggtgc ctgtcgtcaa aggattttcc actgaaagcg
     2041 cggtagaagt ggcttcgtta ggcattcagg tccatggcgg cgtaggcttc atcgaggaga
     2101 ccggtgcggc gcagtactac cgcgatgcac gtattctgcc aatttacgag ggcactacag
     2161 cgattcaggc caacgatttt gtaggtcgca aaacgctgcg cgatggcggg atggtggccc
     2221 gcgccgttat cgaccagatg cgcgccacgg ttgtcgaact cgaagcgcgc gccgccacat
     2281 ccggagctca tcgcgcgggt ctgaccctgg tggcgcggca tctggacgcg gcggtcgtgg
     2341 cctacgaaaa ggcgctggaa tatgtgctgg ccaatgccgc tgagtccttg cgcgcggtgt
     2401 ttctgggcag tgtcccttat ctg
//
LOCUS       DATCXU010000008         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1092706,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000008 DATCXU010000000
VERSION     DATCXU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092706"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIR04_00155"
     CDS             complement(<1..195)
                     /locus_tag="VIR04_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY9459189.1"
                     /translation="MQNLAQLSVDWFLLTLALPKVGLSAVFVVSLLSATLLPLGSEPA
                     VFGFVKLAPHMFWPAVLVAAA"
     gene            291..1799
                     /gene="ilvA"
                     /locus_tag="VIR04_00160"
     CDS             291..1799
                     /gene="ilvA"
                     /locus_tag="VIR04_00160"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY9459190.1"
                     /translation="MPSDYLKRILTSKVYDVAIESPLEFAPQISTRINNRVLLKREDT
                     QAVFSFKLRGAYNKMANLSPAELKRGVIAASAGNHAQGVALSAKRLGCRAVIVMPVTT
                     PQVKIDAVRSYGSEIVLMGDSFSDAYLHAKKLEKKEKLTFVHPFDDPDVIAGQGTVGM
                     EILHQHPGPIDAIFVAVGGGGLISGVAACIKQLRPEIKIIGVQTVDSDAMMRSIKAGR
                     RVELNDVGLFSDGTAVKLVGVETFKLARKYVDDFIAVDTDAICAGIKDVFRDTRSVLE
                     PAGALALAGAKRYAALNNWKNKTIVAITCGANMNFDRLRFVAERADVGEAREAVFAIT
                     LPEERGSFRRLCAAVGRRSVTEFNYRISDSKTAHVFVGLQIHSAAEPDKIAESFRKKG
                     FPTLDLTANEMAKTHLRYMVGGHSPLAVNELLYRFEFPERPGALTKFLDAMNPEWNIS
                     LFHYRNQGDDYGRILVGIQVPPADKKAFKDFLDTLGYPRWNETGNPAYALFL"
     gene            complement(1817..2368)
                     /locus_tag="VIR04_00165"
     CDS             complement(1817..2368)
                     /locus_tag="VIR04_00165"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY9459191.1"
                     /translation="MDIDPAIYVRNTIRSVPDWPKPGVVFRDITPVLQDPRSFRVLVD
                     LFVYRYMRQRLDLVAGIDARGFILGSVLAHELNLGFVPVRKKGKLPYRTLAEEYSLEY
                     GNAAVEMHTDSVRPGQRVLLIDDLVATGGTMMAASKLLQRLGANVVEAAAIIDLPDLG
                     GSRAIKATGMPLYTVCQFSDSAP"
     gene            complement(2469..>3235)
                     /locus_tag="VIR04_00170"
     CDS             complement(2469..>3235)
                     /locus_tag="VIR04_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9459192.1"
                     /translation="VRVRALLDAEPALVDMDSPLALPSSGVLELAAIRYHYPGPPRGH
                     VLDGLNLRVEPGEHIAIVGVSGSGKSTLLSLIMRLFDPDSGMISYGGVPISKVKLAEL
                     HRRFTLLSQRSPVFLGTVRSNLLIGDSSATDRQLWQALEDAGLACFVRSFSVGLDMWV
                     GESGSDLSLGQARRLCLARALLTSATVWVLDEPTAGLDTDAQAAFFSDLRRAAAGRTV
                     VLATHAEIPPGGVDRVLSLSSGILHDGPARYAPPAD"
BASE COUNT          793 a          992 c          866 g          584 t
ORIGIN      
        1 cgccgcggcg accagcacgg ccggccagaa catatgcgga gccagtttca caaacccgaa
       61 cacagcgggc tccgaaccta gcgggagcaa agtcgccgac agcaggctga cgacgaaaac
      121 agcgctaagg cccactttgg gcaaagccaa agtgagcaaa aaccaatcga ccgaaagttg
      181 cgccaaattt tgcatagccc gctagtgtat ccttgaaacc ccggcgaaaa tcaaaaaacc
      241 gctgtggtaa tctattttcc ttacgctacc gctgaatctc tttttacacc atgccttccg
      301 actacctaaa gcgcatcctc acgtcaaaag tttacgacgt ggcgatcgaa tctccgcttg
      361 aattcgctcc ccagatatcc acccgaatta ataaccgcgt gctcctcaag cgcgaggaca
      421 cacaggccgt cttcagtttc aagctgcgcg gcgcctataa caagatggcc aatttaagtc
      481 cggcggaact caaacgcgga gtcattgccg cgtcggcggg caatcacgcg cagggcgtcg
      541 ccttgtcggc aaagcgtctt ggatgtcgtg cggtcatcgt catgccggtc accacaccgc
      601 aagtgaaaat cgacgcggtc cgcagttacg gcagcgaaat cgtattaatg ggcgacagct
      661 tttccgacgc ctatttgcac gcaaagaaac ttgaaaaaaa agaaaaactg acattcgtgc
      721 acccttttga cgatccggac gtcatcgcgg gtcaaggcac cgtgggcatg gagattttgc
      781 atcagcaccc tgggccgata gacgccatct tcgtggcggt tggcggaggc ggcctgattt
      841 caggcgtggc tgcctgcatc aaacagcttc gccccgaaat caaaattatc ggtgtgcaaa
      901 cggttgattc cgacgccatg atgcgaagca tcaaagcggg ccggcgggtc gaactgaacg
      961 atgtcgggct attttcagac gggaccgccg tcaaactggt gggggtcgag actttcaagc
     1021 ttgcgcgcaa atacgtcgac gatttcatcg cggttgacac ggacgccatc tgcgcgggca
     1081 tcaaggatgt ctttcgcgac acgcgcagtg tcctggaacc cgccggggcg ttggcgctgg
     1141 cgggagcaaa gcgttatgcc gcactgaaca actggaaaaa caaaacaatt gtcgccatca
     1201 catgcggcgc caatatgaac tttgaccggc tgcgcttcgt cgctgaacgc gccgatgtgg
     1261 gcgaagcgcg cgaagccgtt tttgcgataa cgctccccga agaacgaggc agtttccgcc
     1321 gcctgtgcgc cgcggtcggg cgccgtagcg tgacagaatt caattaccgc atctccgact
     1381 cgaagacggc ccatgttttc gtgggcctgc aaatccattc ggcggcagag cccgataaaa
     1441 ttgcagaaag cttccgcaaa aaaggcttcc ccacgctgga tttaaccgcc aatgaaatgg
     1501 ccaaaacgca cctgcgctac atggtgggcg ggcattcgcc gctggccgtg aatgaattgc
     1561 tttaccgctt tgagtttccc gaacggcctg gcgccctgac gaaatttttg gatgcgatga
     1621 accccgaatg gaacatcagc ctgttccact accgcaacca gggcgacgat tacggccgca
     1681 ttctggtggg gatacaggtt ccgccagccg ataaaaaagc gttcaaggat ttcctggaca
     1741 cgctgggcta cccgcgttgg aatgaaaccg gcaatccggc ttacgcgtta tttttgtaga
     1801 taaaaccgca agcggatcaa ggggctgaat cggaaaactg acagaccgta tagagcggca
     1861 tgcccgtcgc cttgatcgcg cgcgaaccgc ccagatcggg caaatcgata atagcggcgg
     1921 cctcgaccac gttcgcgccc agtcgctgca gcagtttgct ggccgccatc attgtgccgc
     1981 ccgtcgcgac cagatcgtcg ataagcaata ccctctggcc gggccggacc gaatccgtat
     2041 gcatctcgac agcggcattg ccgtattcaa gcgaatattc ttcggccagt gtgcggtaag
     2101 gcagttttcc tttcttgcgc acaggaacga agcccagatt caattcatgg gccagcaccg
     2161 aacccagaat aaaaccacgc gcgtcgattc cggcaaccag gtcaagccgc tgccgcatgt
     2221 agcgatacac aaacaagtca accagaacgc gaaacgaccg ggggtcttgc agcacagggg
     2281 tgatatcgcg aaatacgacg cccggcttgg gccagtcagg cacgctgcgt atcgtattgc
     2341 gaacgtagat cgcgggatca atatccattt aaatcgccga aagaagttca gaagccacta
     2401 taaccgccga aaagcaggcg aagtttacac ctttacaccg ctcgccagac aacgccgcgg
     2461 gctctgcgtc aatccgccgg cggcgcgtaa cgcgccggac catcatgcag tattcctgaa
     2521 gacaatgaca atacccgatc gaccccgcct ggcggaatct ctgcatgcgt ggccagcacc
     2581 accgtccggc ccgccgccgc ccgtcgcaaa tcggaaaaga aggccgcctg cgcatccgta
     2641 tccagtcccg cggtgggctc gtcaagcacc cataccgtcg ccgaggtcag caaagcccgg
     2701 gccagacaca aacgacgggc ctgccccaac gacagatcgc tgcccgactc gcccacccac
     2761 atatcaagcc cgacactgaa cgagcgcaca aaacacgcca atccagcgtc ttccagcgct
     2821 tgccacaatt gccgatcggt cgcactggaa tcgccaatca gcaaattgct gcgcacggtc
     2881 ccaagaaaaa cgggcgaacg ctgcgaaagc aaggtaaacc ttctatgcaa ttcagccaac
     2941 ttcaccttgc tgatcggcac accgccatac gagatcatgc cgctgtcagg atcaaaaaga
     3001 cgcattatca aagacaacag cgtcgatttt cccgacccgc tgacgccgac tatggcaatg
     3061 tgttcgccgg gctcgacacg cagattcagg ccgtccagca cgtgcccgcg cggtggtccc
     3121 ggatagtgat accgtatggc cgcaagctcc agcacgcccg acgacggtaa cgccagcggc
     3181 gaatccatgt cgactaaagc cggctcggcg tcaagcaacg cgcgcacacg cactc
//
LOCUS       DATCXU010000009         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_312224,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000009 DATCXU010000000
VERSION     DATCXU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312224"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIR04_00175"
     CDS             <1..520
                     /locus_tag="VIR04_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459193.1"
                     /translation="PYFYGAPTNRSQRHTLDVDCPRILDYCRRDGVDVAVLVPNCPVC
                     HQTLSLVARYLEARGIATVIMGCAKDIPEYCGVPRLLFSDFPLGNAAGRPHDPESQIA
                     TLELALRVLESAPAARTTVQSPLRWSDSHEWKLDYCNVERVAPDELAGLRAEADKARE
                     IARELRENKAAS"
     gene            517..1677
                     /locus_tag="VIR04_00180"
     CDS             517..1677
                     /locus_tag="VIR04_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9459194.1"
                     /translation="MNFKLTEQQGQLRDAVRKFAQASLVEIAKTVEEQSMPLPQEWVR
                     KMADMGFLGVNVDQQYGGLGLSHLDAVLVLEEVAKISPAVAFPIFESSFGPMLAIGHF
                     ASEELKRRVMPKVCAGELIVAVSMSEPDAGSALTDLKTRAVISGDKIVINGQKRWCSG
                     AGHSGAYMVYCRLSDQAGAAGIGAVYVEKDTAGLTFGKQERLMGFRGIPSADMFLDNV
                     EVPLSNLIVPAGGFKKLMEAFDLERCGNATMSVALAAGALDYVLDYVRERRQFGKPIM
                     EFQAVQIKLAEMAMRIEAARLLLYQAVVNAQDGLPSIKESSMAKCFANEMVREVCGSA
                     LQLMGGYGYNKDYPIEQRFRDGWGWGIAGGAVDIQKVNIAAALLNKRFDQRR"
     gene            1733..2113
                     /locus_tag="VIR04_00185"
     CDS             1733..2113
                     /locus_tag="VIR04_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9459195.1"
                     /translation="MSQPSWIVKESDVTGYSPANHSGTLNRRLIGRETVGARNVEVLL
                     GVIEKNQGALPHAHPGIEQVCYLLSGSATVQVDGESGEMEAGDCCYFAPGKQHVFTVT
                     SDIPARVLVIYSPPYEENPDRVNR"
     gene            2136..>2316
                     /locus_tag="VIR04_00190"
     CDS             2136..>2316
                     /locus_tag="VIR04_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9459196.1"
                     /translation="MGAVNPSSAGPLEGIRILDLSAVVMGPFATQILGDLGADVIKLE
                     SPSGDDMRGVGSKRHP"
BASE COUNT          480 a          569 c          738 g          529 t
ORIGIN      
        1 gccgtatttt tatggcgcac ccactaatcg cagccagcgt cataccctgg atgtagactg
       61 cccgcgaata ctggattatt gtcgacgcga cggggttgac gtggcggtgc tggtgcccaa
      121 ctgtccggtc tgtcatcaga cattaagcct ggtggcgcgc tatctcgaag cgcgcggcat
      181 tgcaacggtg attatggggt gcgccaaaga tattcccgaa tattgcggtg tgccgcgact
      241 gctgttcagc gacttcccgt tgggcaacgc ggccggccgg ccgcatgatc ctgaatcgca
      301 gatcgccact ctggaattgg cgctgcgtgt attggaatcg gccccggcgg cgcgcaccac
      361 ggtgcaatcg cctttgcgct ggagtgattc acacgagtgg aaactggatt attgcaatgt
      421 tgaacgcgtg gcgcccgatg aactggcagg cttgcgcgcc gaggccgaca aggccagaga
      481 aatcgccagg gagcttcggg aaaataaggc ggcatcatga acttcaaact tactgaacag
      541 caaggacaat tacgcgatgc ggtgcgcaag ttcgctcagg cgtcattagt tgaaattgca
      601 aaaactgtcg aagaacaaag catgcccttg ccgcaagaat gggtgcgcaa aatggcggac
      661 atgggatttc tcggtgtcaa tgtcgatcaa caatacggtg gtctgggctt gagccatctt
      721 gatgcggttt tggtgcttga ggaagtagcc aagatttcgc ccgcggttgc gttccccatt
      781 ttcgagtcat cttttggacc catgctggcg attggccatt ttgcctctga agagcttaag
      841 cggcgcgtta tgcccaaggt gtgcgcgggc gaattgatcg tggccgtaag catgtcagaa
      901 ccggatgccg gcagcgcgct aaccgacctt aaaaccaggg cagtcatcag cggcgacaag
      961 attgttatta acggtcaaaa gcggtggtgt tcgggtgcgg gccattcggg cgcctacatg
     1021 gtgtattgcc gtttgtcgga tcaggccggc gcagcgggta ttggcgctgt gtatgtggaa
     1081 aaggacacgg ccggtcttac tttcggcaag caggaacgcc tgatgggctt tcgcggcatc
     1141 cccagcgccg acatgttcct tgataacgtt gaagtgcccc tatcgaatct gattgtcccc
     1201 gccggcggtt ttaaaaaatt gatggaggcg ttcgacctgg agcgttgcgg caatgcgacg
     1261 atgagcgtgg ctcttgccgc tggcgcgttg gactatgtgc tcgattatgt gcgggagcgc
     1321 cggcagttcg gaaagccgat tatggagttt caggcggtgc agattaagct ggccgaaatg
     1381 gcgatgcgga tcgaggcggc gcgtttgttg ctctatcagg cggtggtcaa cgctcaggat
     1441 ggattgccgt cgatcaagga aagctcgatg gcgaaatgtt ttgccaatga aatggtgcgc
     1501 gaggtttgcg gatcggccct gcaacttatg ggcggctatg gctacaacaa agactatccc
     1561 atcgaacagc gctttcgaga tggctggggt tggggcattg ccggcggcgc cgtcgatatt
     1621 cagaaggtca atattgcggc cgcattgttg aacaagcgtt tcgatcagcg ccggtgaatg
     1681 acggacgcgg gtgtacactt tttgatcgca cggttgcttt cttgggaaaa aaatgagcca
     1741 gccttcctgg attgttaaag agtcggatgt gaccggttat tcaccagcca atcattccgg
     1801 tacgctgaac cgacgcctta tcggccggga gacggtgggt gctcgaaacg tggaagtgct
     1861 cttaggcgtg atagagaaaa atcagggggc cttgccgcat gcccatccag gcatcgaaca
     1921 ggtgtgctac ctgcttagcg gctcagcgac tgtgcaggtt gacggcgaga gcggcgagat
     1981 ggaagccggc gactgctgtt atttcgcgcc gggcaagcaa catgttttca cggtgaccag
     2041 cgatattccc gcgcgcgttc tggttattta ttcccctcct tacgaagaga atccggatcg
     2101 ggtcaatcga tgagagaggc ttgtatggtg ttgcgatggg cgcggtaaat ccgtcttcgg
     2161 cagggccgtt ggagggcatc cggatccttg atttaagcgc ggtggtaatg gggccatttg
     2221 ccacgcagat ccttggcgac ttgggcgccg atgtcattaa actggaatct ccgtccggag
     2281 acgatatgcg cggggtcgga tcgaagcgtc atcccg
//
LOCUS       DATCXU010000010         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_862434,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000010 DATCXU010000000
VERSION     DATCXU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862434"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VIR04_00195"
     CDS             complement(<1..173)
                     /locus_tag="VIR04_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459197.1"
                     /translation="MSGFVESLQYVFAPQIINGLSIGIAVTLVALGLTIIFGLLDVIN
                     MAHGEFYALGAYVG"
     gene            complement(252..1457)
                     /locus_tag="VIR04_00200"
     CDS             complement(252..1457)
                     /locus_tag="VIR04_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459198.1"
                     /translation="MVKVSIIRNALMASIFAGFVSFGGIASAQNSSPIKVGVLTPLSG
                     AYASIGQQVKWGIQLATDEINAAGGIMGRQVELTFEDGEANPAVGTQKAEKLFQSTKV
                     DFLTGTVNSGSTLAIGQIAERNDRLMATTVSFSDAITGDKCSPNVFRVNARAGQQSAA
                     LAQWLAQWMAQGKPNARVFYLGPDYEMGRSTVATFKATAEKKGAKTVGEVFAPLGNKD
                     YSPFFGQLRAAHPDVLYTSVAGNDTVRLFSQMSEFGLLKNLTVVGASGTVTSQNMSAI
                     AKAADGFLTGVGYSTEISTDENQKFVAAFRKAYKVLPDLYGADSYGVMYFYKAAVEKA
                     RSTDTAKLRTAMDNLEWMTPQGKKTMRAGDHQAIQDMYVVKLANGKFIINSKIAGLDA
                     IGPDTCTRF"
     gene            complement(1574..3091)
                     /locus_tag="VIR04_00205"
     CDS             complement(1574..3091)
                     /locus_tag="VIR04_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459199.1"
                     /translation="MLDLGRSFVASVERCPDQIAIVDGELRLTYGQWFERVRNVAGGL
                     HAMGLGHGDHLLTVLQNSWEMATLHWACQFLGAVMTPLNWRAKPEELDYCASNSEAAL
                     IVFQDVSAQTVANSAVAQRLPRVAVQSAPDGVQSFEAMLESKPTERLLNADASDWSLL
                     LFTSGTTGMPKGVPRRHCHERAAALAHVAQNLYRVGERTLGVMPLYHTMGVRSLLSMA
                     LINGRFVCIRRFNAAEALRVIAAEAITNLYLVPTLYHDLAECQDSLRADISSVRKLGF
                     AGAPMNEGLLKRLDLMFKPELFVNHYGSSEVYTYTIEPNARSKPGSAGKSGINQRIRV
                     VKLNAEDANDMAADGEEGQIVADLHGDEAFEGYWKRTDADAKALHGDWYFTGDLGYFD
                     EQGDLFVTGRIDDMIISGGENISPTDIESLLSLHPAVDEVAVVGLPDERWGQKVSAFV
                     KLKNDVDAAELDKYCRQSSLPDFKRPREYVFVSGLPKSPVGKILRRKIVAGEYER"
     gene            complement(3084..3602)
                     /locus_tag="VIR04_00210"
     CDS             complement(3084..3602)
                     /locus_tag="VIR04_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY9459200.1"
                     /translation="MKRYDKAVKHVITLTLNGMTRTGHAESRMLLVDFLRHELGVTGT
                     HVGCEHGVCGACTVLVNGVATRSCLQLAVQSDGMHIETVEGLNRDCPGELGDLQQAFK
                     SHHALQCGFCTAGILMSCTDFLKRVSMPTRQQVTEMLSGHLCRCTGYTNIVDAVMDVA
                     TQRMEKKRVEHA"
     gene            complement(3577..>4237)
                     /locus_tag="VIR04_00215"
     CDS             complement(3577..>4237)
                     /locus_tag="VIR04_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY9459201.1"
                     /translation="DYSRIEGGFLTIGAAATQASVQGRTALATEVPLLEKVFPSISHF
                     QIRNRGTVCGSIAHADPSAELPLCLTALGGEVVLRSSMGRRVLKANEFFVGMLITALR
                     PGELVEEVRFPLADKRHSYAFEEVTMRHGDFAIAALAADVTDKDIALSVGGVADRPVR
                     KVWPILSEVELDAGLNDFAWELQAQHDFHATAKYRRHLVRQLGRKLIIRARDETLRQS
                     C"
BASE COUNT         1069 a         1257 c          970 g          941 t
ORIGIN      
        1 ccgacatagg cgcccaaagc atagaactcg ccatgagcca tatttatgac atcaagcaat
       61 ccaaaaataa ttgtcaaacc caatgcaacc aaggtaacgg caattccaat ggacaatccg
      121 ttaataattt gcggtgcaaa aacatactgt agcgattcaa cgaatccgct catgccaaca
      181 atcccccaac caagaattcg ccgcaaacgc aggctccagc caaccccgga accttggaaa
      241 aacgcgcccg atcagaagcg agtgcaagtg tccggaccaa tggcatcaag gccggcgatt
      301 ttgctattga taataaattt accgttggca agcttcacta catacatgtc ctgaattgcc
      361 tggtggtcgc ccgcccgcat cgtttttttg ccctgcggcg tcatccattc aagattgtcc
      421 atagccgtac gcagcttcgc cgtgtcggtg ctacgagcct tctcgacagc cgccttgtaa
      481 aaatacatga caccataaga atcagcgcca tacagatccg gcagaacctt gtaagccttt
      541 cggaacgccg cgacaaattt ttgattttca tcggtgctga tctcggtcga atagccgact
      601 cccgtcagga acccgtcggc tgcttttgca atagcgctca tgttctgcga cgtgacggta
      661 cccgacgcgc ccactacagt cagattcttg agaaggccga attcgctcat ttgggaaaat
      721 aggcgaacgg tatcgttgcc tgctactgat gtatacagga cgtcgggatg agcggcgcga
      781 agctggccga agaaaggtga ataatccttg ttgcccaatg gggcaaatac ttctccaact
      841 gtcttcgccc ccttcttttc cgccgtggcc ttgaacgttg cgaccgtgct tcgccccatc
      901 tcgtagtccg ggcccagata aaacaccctt gcgttgggtt taccttgtgc catccactgc
      961 gccaaccatt gggccaacgc agccgattgc tggccggctc tggcattgac gcgaaacaca
     1021 ttcggcgagc acttgtcacc cgtgattgca tctgaaaaag aaaccgtggt ggccatcaaa
     1081 cgatcattgc gctcggcaat ctggccaatc gccaatgtgg atccggagtt tacagttccc
     1141 gtcaggaaat ccaccttcgt cgactggaac aatttctcgg ccttttgagt gcccactgcg
     1201 gggtttgctt ccccgtcttc gaacgtgagt tccacctggc ggcccataat gccgcccgcc
     1261 gcgttgattt cgtccgttgc aagctgaatg ccccatttca cctgctgacc aatgctggcg
     1321 tatgcacctg aaagcggggt cagtacgccg accttgatgg ggcttgaatt ctgagccgat
     1381 gcgatgccac caaaactgac gaagccagca aatattgacg ccatcagcgc attacgtatt
     1441 atcgatacct ttaccatgat gtctcccttt tttatctgat gacaaactaa acagcccgaa
     1501 tacgtcgagc tatcccgtgc aatcccttaa atttccataa ttaaaggagt cccaatgcga
     1561 acaaaaagaa gaactatctt tcgtattcgc ctgcaacaat tttgcgcctc aagattttgc
     1621 caacaggcga ctttggaagc ccgctgacaa acacatattc acgtggcctt ttaaagtccg
     1681 gcaagctaga ttggcggcaa tatttatcca attccgcagc gtcaacatcg tttttaagtt
     1741 ttacgaacgc cgacaccttc tggccccagc gttcatcagg taaacccacc acagcaactt
     1801 cgtccaccgc cgggtgcaga gatagcaggc tttctatatc ggtcggcgaa atattttcgc
     1861 cgccactgat aatcatgtcg tcgatgcgac ctgtcacaaa caaatcgccc tgttcatcaa
     1921 aatagcccaa atcacccgtg aagtaccagt cgccatgcag ggctttcgcg tccgcgtcgg
     1981 ttcgtttcca atacccctcg aaggcctcgt cgccatgcag atcagcaaca atctgccctt
     2041 cctcgccgtc ggcggccatg tcgttggcgt cctcagcatt caatttcaca acccggattc
     2101 gttgattgat acccgatttg cccgcgctgc cgggcttact gcgagcattc ggttcaatcg
     2161 tgtaggtgta cacctccgaa gagccataat gattaacaaa caactctggc ttgaacatga
     2221 gatccagccg tttcaacaaa ccttcgttca ttggcgcacc ggcgaagccc aacttgcgca
     2281 ccgaggatat atccgcccgc agggaatctt ggcattcggc aaggtcatga tataaagttg
     2341 gcactaaata caaattggta attgcctcgg ccgcgatcac ccgcagggct tcggcggcgt
     2401 taaatctgcg aatacatacg aaccggccat ttattaaggc cattgaaagg agcgaccgca
     2461 ctcccatcgt gtgataaagc ggcatcacgc ctaaggtgcg ctcacctacg cgatacaaat
     2521 tttgcgcaac atgcgccagg gccgcggcac gctcatgaca gtgacgacgc ggcacacctt
     2581 taggcatccc agtggttccc gaggtaaaca acagcaggga ccaatcactc gcatcagcgt
     2641 tcagcagccg ttctgtcggc ttagattcga gcatcgcctc gaacgactgc acaccgtccg
     2701 gcgcagattg gacagccaca cgcggcaaac gctgagcgac ggccgagtta gcgactgttt
     2761 gtgccgagac atcctggaac acgatcaaag cggcttccga attagaagca caataatcca
     2821 gctcctcggg cttggcacgc caattaagag gcgtcataac agcacccaaa aactggcaag
     2881 cccagtgcaa ggttgccatt tcccaactat tttgcaacac tgtcagtaaa tgatcaccat
     2941 gcccaagccc catggcatgc aaccctccgg ccacgttgcg aacacgttca aaccactgtc
     3001 cgtaagttaa acgcaattca ccatccacta tggcaatctg gtcgggacat ctttccaccg
     3061 atgctacaaa actgcggcct aaatcaagca tgttccactc gcttcttctc catacgctgt
     3121 gttgcaacat ccatcacagc atcaacaata ttggtatagc ccgtgcaacg gcacaaatgg
     3181 ccggatagca tctcagtcac ctgctgacgg gtcggcatac taacgcgctt aagaaaatca
     3241 gtgcacgaca tcagaatccc ggctgtgcaa aaaccacact gcaaggcatg atgagatttg
     3301 aatgcttgct gtaaatcgcc aagttcgccg gggcaatctc gattcaaccc ctcaaccgtt
     3361 tcaatatgca tgccatcgga ctgaaccgct aattgcaaac aggaacgcgt tgcgacgcca
     3421 ttaaccagaa cagtacaggc gccgcaaacg ccatgctcgc agccaacatg ggtgcccgtg
     3481 acgcccagtt cgtggcgcaa gaagtcgacc agcaacatgc gcgattctgc atgcccggta
     3541 cgcgtcatgc catttagtgt caatgtgatt acgtgcttaa cagctttgtc gtagcgtttc
     3601 atcccttgcc ctgataatta attttcgtcc aagttgacgc accagatgtc ggcggtattt
     3661 tgcagtcgca tgaaagtcgt gctgcgcctg gagttcccag gcgaagtcat ttagaccagc
     3721 atccaactcc acttcagaca atatcggcca aaccttgcgc accggacggt ccgcaacacc
     3781 accgactgac aatgcaatat ccttatcagt gacgtccgca gccaatgccg cgatagcaaa
     3841 atctccgtga cgcatcgtca cctcttcaaa cgcataactg tggcgtttgt cggccaatgg
     3901 aaagcgcacc tcctcgacaa gctcgcccgg cctaagcgct gttatcaaca taccgacaaa
     3961 gaattcattg gcctttaata cgcgacgacc cattgacgaa cgcaatacga cctcgccgcc
     4021 cagcgcggtt aaacaaagcg gcaattcagc gctgggatcg gcgtgcgcta ttgagccaca
     4081 caccgttccg cgattacgga tctggaaatg agatatggac ggaaatacct tttccagtag
     4141 cggcacctcg gtcgccagcg cagttcgccc ttggacactg gcttgagtgg ccgccgcccc
     4201 aatcgtaaga aaaccccctt cgatcctgga ataatca
//
LOCUS       DATCXU010000011         5580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_72873,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000011 DATCXU010000000
VERSION     DATCXU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72873"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="glcE"
                     /locus_tag="VIR04_00220"
     CDS             <1..271
                     /gene="glcE"
                     /locus_tag="VIR04_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY9459202.1"
                     /translation="QPPPLNLGPTLMEWSGGQRWFANPDSGADLRLPVARAGGHATLF
                     RYASKAADVPAFHPPGAGLMNISRRMKHQLDPSGIFNPKRLFPEF"
     gene            281..1516
                     /gene="glcF"
                     /locus_tag="VIR04_00225"
     CDS             281..1516
                     /gene="glcF"
                     /locus_tag="VIR04_00225"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY9459203.1"
                     /translation="MQTNLVSWARESDIGQDADDILRTCVHCGFCLATCPTYQILGDE
                     RDSPRGRIYLIKQVLEGAEPTAATQEHLDRCLTCHNCETTCPSGVKYGHLVDIGRKLV
                     DERVKRPLTQRVTRWLLRNLLNSSLFGPAFKLGRMARPLLPSVLKNKIPAARPAGLLP
                     DGAHHKRQVLLLASCVQPAMMPSIDAATIRVLDAIGIGTQIVPGAGCCGAINFHLDAQ
                     EEALNQMRANIDAWLPMLDDGSIEAVVMNASGCGATVKEYAHHLRHDPAYFDKAARVV
                     EKVLDIAEIVAPHTAELRAKMPTFSERVAFHPPCTLQHWQGLRDVSEQVLMDLGFELL
                     PFADSHLCCGAAGTYSVTQPKLSKELRHRKLEAIQEARPGRIVSSNMGCIGHLQSGTA
                     TPVRHWIEVVDEALAQRRT"
     gene            1516..2625
                     /gene="mnmA"
                     /locus_tag="VIR04_00230"
     CDS             1516..2625
                     /gene="mnmA"
                     /locus_tag="VIR04_00230"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY9459204.1"
                     /translation="MSSASKGRVVVGMSGGVDSSVAAWLLKERGYDVLGLFMKNWEDD
                     DDSEYCSSRQDWLDAVSVADLIGVDIEAVNFAAEYKDRVFSDFLREYSAGRTPNPDVL
                     CNAEIKFKAFLDHAMSLGAEYIATGHYARVRTVQSQDGPRFELLKALDLTKDQSYFLH
                     RLTQSQLAKTLFPLGQIPKTEVRRIARSLRLPNAAKKDSTGICFIGERPFRAFLSRYL
                     PTRPGPIKTTDGDIVGQHQGLSFYTLGQRKGMGIGGVKGRQHDDGTSDAWYTARKDLE
                     RNTLYVVQGHQHPWLLSSRLQAQDASWIAGCAPAPGPYGAKTRYRQADAACGLQTMAP
                     DGFELNFSESQWAVTPGQSAVLYAGEVCLGGGIIL"
     gene            complement(2636..3250)
                     /locus_tag="VIR04_00235"
     CDS             complement(2636..3250)
                     /locus_tag="VIR04_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY9459205.1"
                     /translation="MKLIGSLTSPYVRKVRVVLAEKKLDYEFVLENVWADNTQIQVHN
                     PLGKVPCLLMDDGGSLFDSRVIVEYLDTLSPVGRLIPQPGRDRAATKCWEAVADGALD
                     AAIVIHTEQTQRPPELRSEQWISRQYKKIWAALKFMDESLDSQPFCMGVNFGLADVGV
                     GCALGYLDLRFGHLNWRDQYSNLLRLEEKLSTRPSFVSTRPSKG"
     gene            3453..4829
                     /gene="purB"
                     /locus_tag="VIR04_00240"
     CDS             3453..4829
                     /gene="purB"
                     /locus_tag="VIR04_00240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY9459206.1"
                     /translation="MHIIEQPSRLNALSPLDGRYAAKTGTLRETLSEAGFMAHRVEVE
                     VAWLLGLSDAGLPELSPFSAQARALLRRLVDDFSEHDAARIKEIEKVTNHDVKAVEYW
                     LKERVADCAELAKAGEFIHFGCTSEDINNTSHALMLTRVRAGFIEPRLDELCEYLKSL
                     ARQFAAQPMLARTHGQPATPTTMGKEFANIAARLERALAAIKAVAPLAKLNGATGNYN
                     AHMAAYPEVDWPAFSRKVLADLGLTQNPYSIQIEPHDWMAALFDAVARANTIVLDLDR
                     DMWGYISLGYFKQSLKEGEVGSSTMPHKVNPIDFENSEGNLGLANAILRHLAEKLPLS
                     RWQRDLTDSTVLRNLGVAFGYCAVAWDSCLRGLKKLEINRAAIDADIDNCWEVLAEPV
                     QTVMRRYGLPSPYEQLKALTRGKRIDQHALREFIAALDLPPEPKARLLALTPRTYIGL
                     AAELARQI"
     gene            4893..4968
                     /locus_tag="VIR04_00245"
     tRNA            4893..4968
                     /locus_tag="VIR04_00245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4926..4928,aa:Lys,seq:ctt)
     gene            complement(5177..>5580)
                     /locus_tag="VIR04_00250"
     CDS             complement(5177..>5580)
                     /locus_tag="VIR04_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY9459207.1"
                     /translation="ATLADTDEAGRILDCAFVVYSPEAKQQCLAVKQKTIERFNLTSE
                     EVAREMALGALGNSRANVVIANTGVTDDTDKDIPAGTQCFAWAFANTPGGHVSLFSET
                     RRFEGQRNQIREASAEYALGRIPHYAKRAAG"
BASE COUNT         1161 a         1531 c         1612 g         1276 t
ORIGIN      
        1 ccaacccccg ccgttgaatt tgggccctac cctgatggaa tggagtggcg gccagcgctg
       61 gtttgcgaac cccgatagcg gcgccgattt acgtctgccc gtggcgcgcg cgggcgggca
      121 tgcgaccctg tttcgttatg cgtccaaagc cgccgatgtt cccgcctttc atccgccagg
      181 cgctggttta atgaatattt cccggcgcat gaagcaccaa ttggatccgt cgggcatttt
      241 taatcccaaa cgtctgtttc ctgaattttg agataatact gtgcaaacaa atttagtatc
      301 gtgggcgcgc gaaagtgaca ttggccaaga tgccgacgac attttgcgta cgtgtgtcca
      361 ttgcggcttc tgccttgcaa cgtgcccgac ctatcaaata ttaggcgacg agcgcgacag
      421 tccgcgcggg cgtatttatc tgatcaaaca ggtgctcgag ggcgccgagc cgacggcggc
      481 cacgcaggaa cacctggacc ggtgcctgac ctgccacaac tgtgaaacga cctgtccatc
      541 gggcgttaaa tatggtcatc tggttgacat aggccgcaaa ttggtcgacg aaagggtaaa
      601 gcgcccgctc actcaacggg taactcgctg gctgttgcgc aatttgttga attcctcctt
      661 gttcggaccc gcctttaaat tgggccgtat ggcgcggcct ttgcttccct ctgtcctcaa
      721 aaacaagatt cccgccgcac ggccggcagg gctgcttccg gatggtgcgc atcacaagcg
      781 ccaagtgctg ttgctggcca gttgcgttca gcctgccatg atgcccagca tcgacgcggc
      841 cacgatccgg gttttggatg ctattggcat cggcacgcaa atcgttcctg gggcgggatg
      901 ttgcggcgcc atcaatttcc atctcgacgc gcaggaagaa gctctgaatc agatgcgcgc
      961 caatattgac gcctggctgc ctatgcttga cgatggttcg atcgaagctg tcgttatgaa
     1021 tgccagcggc tgcggcgcga cagttaaaga atatgcgcat cacctgcgcc acgatccggc
     1081 gtatttcgac aaggcggcgc gcgttgttga aaaggtgctc gatatcgccg aaattgtggc
     1141 gccgcacacg gccgaactca gggcgaagat gccaacgttc tcggagcggg tggcttttca
     1201 ccctccatgc accttgcagc attggcaggg gttgcgcgat gtcagtgaac aagtgctgat
     1261 ggacctggga tttgaacttt tgccttttgc cgattcgcat ttgtgttgcg gtgcggcggg
     1321 cacttattcg gtgacgcagc cgaaactttc caaagaactg cgccatcgca aattggaggc
     1381 catacaggaa gcccgtcccg ggcgcatcgt atcgtcgaat atgggttgta tcggacattt
     1441 gcaaagcggg actgcgacgc ctgtgcgaca ttggatcgaa gtcgttgacg aagcccttgc
     1501 gcagcggcgg acctgatgtc atcggcatcg aaaggacgtg tggttgtcgg catgtccggc
     1561 ggcgtcgact cttcagtggc tgcatggctg ctgaaggagc ggggctacga tgtcctgggt
     1621 ctgttcatga agaactggga agacgacgac gattcggagt attgttccag ccggcaggac
     1681 tggcttgatg ccgtcagcgt cgccgatctg attggtgtcg atatcgaggc tgtcaatttt
     1741 gcggcggaat acaaagaccg ggtattcagc gactttctgc gcgagtactc ggccggccgt
     1801 acgcccaatc ccgatgttct gtgcaatgcc gaaatcaagt tcaaggcttt tctggatcat
     1861 gccatgtcgt tgggcgccga atatatcgcc acaggtcatt acgcacgggt gcgcacggtg
     1921 caaagccagg atgggccgcg gttcgaattg cttaaagcgc tcgacctgac caaggaccaa
     1981 agctatttcc ttcatcgcct gacccagtct caattggcta aaactctgtt tccgttgggc
     2041 cagatcccca agaccgaagt tcgccgaatc gcgcggtctc tgcgcttgcc caatgccgca
     2101 aaaaaagact cgaccggtat atgttttata ggggaacggc cttttcgcgc gtttttaagc
     2161 cgttatttgc ccaccaggcc cggaccgatt aaaacaaccg atggggatat tgtcgggcag
     2221 caccaggggt tgtcatttta tactttgggc cagcgtaaag gcatgggtat tggcggggta
     2281 aaaggcaggc aacacgacga tggcacatcg gacgcgtggt acacggcgcg caaagatctt
     2341 gaacgcaaca ctctgtatgt tgtgcaaggg caccagcatc cgtggctgtt aagttcgagg
     2401 ttgcaggcgc aagatgccag ttggattgcc gggtgtgcgc ccgctccagg cccttatggg
     2461 gccaaaactc gctatcgtca ggccgacgcg gcgtgcgggc tccagacaat ggcgccagac
     2521 ggctttgaat tgaattttag cgagtcgcag tgggcggtta caccaggcca gtctgcagtg
     2581 ctttatgccg gcgaggtgtg cctgggcggc ggaatcatcc tatagcgtgt ggactttagc
     2641 ccttggacgg acgcgtgctg acaaaagatg ggcgggtgga cagcttttct tccagccgga
     2701 gcaaattgct gtattgatcg cgccaattca aatggccaaa acgcaagtcc aggtaaccta
     2761 acgcgcagcc cacgccaaca tcggccaggc caaagttgac ccccatgcaa aagggttgcg
     2821 aatccaggct ttcatccatg aatttcagcg cggcccagat ctttttgtat tggcggctga
     2881 tccactgttc gctgcgtaat tccggcggcc gctgggtttg ttctgtatga atgactatgg
     2941 cggcatccag tgctccgtcg gcaacggctt cccaacattt agtggctgcg cgatcccggc
     3001 ccggttgcgg gataagccgg cctaccggcg acagggtatc aaggtattcc acgataaccc
     3061 gggaatcaaa caaactgccg ccatcgtcca taagcagaca aggcaccttg cccagaggat
     3121 tgtgcacctg tatttgcgtg ttgtcggccc agacattctc aagaacgaat tcgtaatcca
     3181 gttttttttc agccaatacg acacgcactt tacgaacgta ggggctagtt agcgaaccaa
     3241 ttaatttcat ggcggggccg ggaagttgaa agcaaaaaaa gtatagcagg ataccgtcgc
     3301 ccaatagcca ttgaacttgc acaatgcgta tgggactcgc gggcaggcgc aaatgtgtgg
     3361 tttatatgcg gcttattcgc gcaaacgcgg cgccggcgct gagggatgtc aatgttagaa
     3421 ttcaggctca tttccttgtt acgtgagctg taatgcatat tatcgagcaa cccagtcgcc
     3481 tgaacgccct gtctccgctg gatggccgtt acgcggccaa gacggggacg ctccgggaga
     3541 cactgtctga agcaggtttc atggcccacc gcgtcgaggt ggaagtggct tggctgcttg
     3601 gtttgtccga cgccggtttg cccgagcttt cgcctttttc ggcccaggcg agggcgcttt
     3661 tgcgccgtct ggtcgatgac ttttccgagc acgacgcagc gcgcatcaaa gaaattgaga
     3721 aggtcacgaa tcacgacgtc aaggcggtcg agtattggct taaagagcgt gtggccgatt
     3781 gcgccgagct ggccaaggca ggcgagttca tccatttcgg ctgtacttcc gaagacatca
     3841 acaacacgtc gcacgcttta atgttgaccc gggtgcgtgc cggctttatc gaacctcgcc
     3901 tggacgagct ttgcgaatat ttgaagtctc tggcgcgtca gtttgccgct caacccatgc
     3961 tggcccgcac acatggccag ccggccactc ccaccacaat gggcaaagaa tttgccaata
     4021 tagccgcgcg cctggagcgg gcgcttgcgg ccatcaaggc ggttgctccg ctggcaaaat
     4081 taaacggcgc cactggcaat tacaacgccc acatggcagc gtatcccgaa gtggactggc
     4141 cggcgttcag ccgcaaggtt ttggccgact tgggtttgac gcagaacccc tactcaatac
     4201 aaatcgagcc ccacgactgg atggcagctt tgttcgacgc tgttgcccgc gcaaacacga
     4261 tagtgcttga tctggatcgc gacatgtggg ggtacatctc tttgggctat ttcaagcaaa
     4321 gcctgaaaga gggcgaagtt ggctcatcga ccatgccgca taaagtcaat cccattgatt
     4381 ttgagaactc tgaaggaaat ctggggctgg ctaacgcgat attgcgccat ttggccgaga
     4441 aactgccact gtcgcgctgg cagcgcgacc tgaccgattc caccgtgttg cgcaatctgg
     4501 gcgtggcatt cggatattgc gccgtcgcct gggattcctg cctgcgcgga ctaaaaaagc
     4561 ttgaaatcaa tcgcgccgcc attgacgccg acattgataa ttgctgggag gttctggccg
     4621 agcctgtcca gactgtcatg cgccgctacg gcttgccttc accctatgaa cagcttaaag
     4681 cgcttacgcg cggcaagcgg atcgatcagc acgcgctgcg ggaatttatt gccgcgctgg
     4741 atttgccgcc cgaaccgaag gcaaggctat tggcgctaac gccgcgtact tacattggtt
     4801 tggcggcgga actggccagg caaatttagt gctgatcggg cccattggcg cgagattcgt
     4861 tttcgtttta caattccccc cgtcgtgaga ccgggctgtt agctcagttg gtagagcagc
     4921 ggactcttaa tccgtcggtc gacagttcga gcctgtcaca gcccaccagt gaaatcaagt
     4981 acttaggccg cattatgcgg ccttttgctt tttgggggtg ggggatttct tgccaatctt
     5041 tgccagtgct tcccccagcg ccttagtcgc aagctggctg taccgcttcg tactggctgc
     5101 cgacctcgca ttcaggcaac atgtggtgaa tggaaaagcc cgagccatgg cgggcagctc
     5161 agtaatctaa agccgcctaa ccagcggctc ttttggcgta gtggggaatg cgcccaaggg
     5221 cgtattcggc acttgcttcg cgaatttggt ttcgttgccc ctcgaaccgg cgtgtttcgc
     5281 taaagaggct gacatgcccg cccggtgtat tggcaaaagc ccatgcaaag cactgtgtac
     5341 ctgcagggat gtctttatcc gtatcatccg tcacgcccgt attcgcgata actacgtttg
     5401 cgcggctatt gcccagtgcg cccagagcca tttcgcgcgc tacttcctcg ctcgtcaggt
     5461 tgaaccgctc aattgttttc tgcttaaccg ccagacattg ctgcttggct tctggtgaat
     5521 agacgacgaa ggcacaatca agaatccggc cggcctcatc agtatcggcc aatgtggcgg
//
LOCUS       DATCXU010000012         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_783408,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000012 DATCXU010000000
VERSION     DATCXU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783408"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VIR04_00255"
     CDS             complement(<1..676)
                     /locus_tag="VIR04_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY9459208.1"
                     /translation="MNRYPLWKYLTVLAALIIGLLYTLPNFFGESPAVQVAGAKSTVK
                     VDNEVLNKVQAVLKGDNIESTGAYFEENGPVGTVRVRFSSTDVQLKGKDAIEKALNPD
                     PANPGYTVALNLLPASPNWLSAIGAHPMYLGLDLRGGVHFLLQVDMHGALTGRYDSLA
                     SDIRTSLREANVKAANVERDNLSITSVFDSADARSEATSTLRRKMPDMVFTNGDESGG
                     HFVLTAK"
     gene            complement(673..1020)
                     /gene="yajC"
                     /locus_tag="VIR04_00260"
     CDS             complement(673..1020)
                     /gene="yajC"
                     /locus_tag="VIR04_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY9459209.1"
                     /translation="MPAVNMPSLVIAQVAGAGGDNALMGMLPIILMFVILYFLMIRPQ
                     MKRQKDHRKMVAALAKGDEVITSGGLLGKVTKVSDTYITIEISELADKPIETVLQRTA
                     VTSVLPKGTIKAL"
     gene            complement(1073..2209)
                     /gene="tgt"
                     /locus_tag="VIR04_00265"
     CDS             complement(1073..2209)
                     /gene="tgt"
                     /locus_tag="VIR04_00265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY9459210.1"
                     /translation="MTGLQFDLLATDGLARRGRMVLNHGVVETPIFMPVGTYGSVKAM
                     LPHELEEAGAQIVLGNTFHLWLRPGTEVLEKHNGLHGFMQWSKPILTDSGGFQVFSLD
                     GLRKITEEGVKFASPIDGSRLFLSPEESMRIQRAMNSDIAMVLDECTPYAIDGRPATI
                     EEAARSMRLSLRWARRSLDAFRQQENPNALFGIVQGGMFESLRDESLAGLVDIGFQGY
                     AIGGLSVGEPKAEMQRILAHIAPRLPIEAPRYLMGVGTPEDLVEGVAQGVDMFDCVMP
                     TRNARNGWLFTRYGDLKIRNARYRDDTRPLDPSCACHTCQHFSRAYLHHLQRANEITG
                     ARLNTMHNLHFYLAIMEEMRNAIETGAFSGWREEFAENRAKGIS"
BASE COUNT          475 a          607 c          678 g          516 t
ORIGIN      
        1 gcttggccgt caatacaaaa tggccgccgc tttcgtcgcc attggtgaag accatgtccg
       61 gcattttacg gcgcaaggtc gacgttgctt ccgagcgcgc gtcggcgctg tcgaagactg
      121 acgtgatgga caggttgtcg cgctcgacat tggccgcctt gacattggct tcgcgtaacg
      181 aagtgcggat atcgctggca agcgaatcat agcggcccgt cagggcgccg tgcatatcga
      241 cctgaagtag aaagtgcacg cctccgcgca agtccaggcc caggtacatg gggtgggctc
      301 caattgctga aagccagttg ggcgaagcgg gcagcagatt cagggcgacg gtgtagccgg
      361 ggttggccgg gtcggggttg agcgctttct cgatagcgtc tttacccttc agttgcacgt
      421 cggtggaaga gaatcgtacg cgcacggttc cgacggggcc gttttcctcg aaataggcgc
      481 ctgtcgattc gatgttgtcg cctttcagga cggcctgaac cttgtttagc acctcgttgt
      541 ccactttaac ggtggatttg gcgcccgcga cttgcacggc gggcgattcg ccgaaaaaat
      601 tgggcagcgt atacagcaac ccgatgatca gggcggcgag taccgttaaa tatttccaga
      661 gaggatagcg gttcatagtg ctttgatggt gcccttgggc aaaaccgagg ttacagcggt
      721 ccgttgcaag acagtttcga tgggtttgtc cgcaagttcg ctgatttcaa tggtgatgta
      781 tgtatcgctg accttggtga ccttgccaag caggccgccg gatgtgatca cttcgtcgcc
      841 tttggccagc gcggcgacca ttttgcgatg gtctttctga cgtttcattt gcggccgaat
      901 catcaagaaa taaaggatga cgaacatcaa gataatgggc agcatcccca ttaatgcgtt
      961 gtcgccgccg gcgccggcga cttgggcgat aacaaggctg ggcatattaa ctgcgggcat
     1021 tcgatgctcc tgaaatgttt ttggttaact tggtattgta tcgaaatctg ggttacgaaa
     1081 tacctttcgc gcgattttcc gcgaattctt cgcgccagcc cgaaaatgcg ccggtttcga
     1141 tggcgttgcg catttcttcc ataattgcca gataaaagtg cagattatgc atagtattga
     1201 gccgtgcgcc ggtgatctcg ttcgcacgct gcaaatgatg gaggtaagcc cgtgaaaaat
     1261 gctggcaggt gtgacaggcg cacgacgggt ctagcgggcg agtgtcgtcc cgatagcgcg
     1321 cattgcggat tttaaggtcg ccgtaccggg tgaagagcca cccattgcga gcattgcgcg
     1381 ttggcatgac gcaatcgaac atgtcgacgc cctgtgcaac gccttcaacc aggtcttcgg
     1441 gagtacccac gcccattaaa taacgtggcg cttcgatggg cagacgaggt gcgatatggg
     1501 caagtatgcg ctgcatttcg gccttgggct cgcccactga aagcccgccg atggcgtagc
     1561 cttgaaagcc gatatcgacc aggccggcaa gagattcgtc gcgcagactc tcgaacatgc
     1621 ctccctgcac gatgccgaat aacgcattgg ggttttcctg ttgccggaag gcgtccagcg
     1681 accggcgagc ccagcgcagt gacaggcgca tcgaacgggc ggcctcttct atagtggcgg
     1741 ggcgtccgtc aatcgcgtac ggcgtgcact cgtcaagcac catggcaatg tcggaattca
     1801 tggcgcgctg aattcgcatg gactcttccg gactgagaaa caaacggctt ccgtcgatgg
     1861 gcgaggcgaa cttaacccct tcttccgtga tcttgcgcaa gccatccagg ctgaaaacct
     1921 gaaatccgcc cgagtcggtc aagatgggtt tggaccattg catgaaacca tgcaggccat
     1981 tatgtttttc cagaacctcg gtgccgggcc gcagccacaa gtggaatgta ttgcccagaa
     2041 caatttgtgc gccggcttct tccagttcgt gcggaagcat ggctttgacg ctgccgtagg
     2101 ttccaacggg catgaatatc ggggtttcga ccacgccatg gttcaacacc attcgccctc
     2161 gccgcgccag gccgtcggtg gccagaagat cgaattgcag cccagtcatt atttcggaga
     2221 ctctatgaac atggcatcgc cgtagctgaa gaaacgatat ctgtccgcga cggcgt
//
LOCUS       DATCXU010000013         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_703264,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000013 DATCXU010000000
VERSION     DATCXU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703264"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1515)
                     /locus_tag="VIR04_00270"
     CDS             complement(331..1515)
                     /locus_tag="VIR04_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY9459211.1"
                     /translation="MKDKNNWRDETLVLHGDAALSTDSSIVAPIHYSATFRAESAEDF
                     VEKANTVRPSAYYTRYGNPVHKRVEAILAELESTETAMVMASGMGAISTTVLSLVSRG
                     DHVIAQKRHYMSTSKLFEEVLPRFGVEVSIVDQTDLSAMKAAIRPQTRLIMVETPVNP
                     TLSLTDLAAVAEMARERGITTIADNTFASPINQKPHALGIDIVVHSATKYFGGHHDVT
                     AGAVCCSNSIAERIWHMHITLGAVLSPMDAWLLLRGLRTLSLRMERINANALALAHWL
                     EEQPQVERVFYPGLQSHPQHELALRQMKGFGAVIAFSLKGGFALTSRFVAALRLATHA
                     VSLGGVETLAVHTAAMWAGTMTEDQMKSAGIEPNFVRMSVGVEHIDDLKADLKQALES
                     VH"
     gene            2252..2968
                     /locus_tag="VIR04_00275"
     CDS             2252..2968
                     /locus_tag="VIR04_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9459212.1"
                     /translation="MSARIVQQVLYLDVADRLRAMIQAQVLAAGAWIDETKLTAELGI
                     SRTPLREALKVLANEGLVRLEPRRGCFVNELSARDLDQIFPLMAMLEGRCAFEAAQKA
                     SVSDLRKLEPLHIELQAYASLEHIDQYYALNARIHEAVQALADNRWLSSLIDNLRKVL
                     SLSRHQSLVAPGRIHESCAEHLAIFAALKARDAQAAEALTRGHLMHQLDALHVLLNQA
                     DPDTGALESSSSIGYSGADL"
     gene            2965..>3176
                     /locus_tag="VIR04_00280"
     CDS             2965..>3176
                     /locus_tag="VIR04_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEY9459213.1"
                     /translation="MTDSGTREPESKPAEADRENLPVAANLLARLGRWWERERWPGPA
                     ETKTLFSARLTDVAANRLAQTWCERYG"
BASE COUNT          675 a          847 c          885 g          769 t
ORIGIN      
        1 ttgatgggtt ctgctgcgcg cgctgtccgg atgaatggcc ccgcaatacc cgataatagc
       61 ccggccgccg cgcccgcctt aagtacagaa cgccgggtaa cgcctgcaat tgtgtttctg
      121 ctttctttcc tgtcagacat aaaccatctc ctgagagagc ctgccgacag atgtgattga
      181 cgagaggctt gttatttctt accagccgaa agtgcgtacc acaggaaggc agaataaact
      241 tggtttatga cactgtcaaa attttttgaa atttttttta gccctcatgc aaagggacta
      301 ggatcaaaga gcacggtgtg ccgccgctat ttaatgcact gattccagtg cttgtttcaa
      361 atccgccttc aggtcgtcga tgtgttcgac acccaccgac atacgcacaa aattgggttc
      421 aatacccgcc gatttcattt gatcttcggt catcgtaccg gcccacatgg ctgccgtgtg
      481 aaccgccagg gtctcgacgc cgcccaggct taccgcgtgg gttgccagtc gaagcgcggc
      541 tacaaagcgg ctggtgaggg caaagccgcc ttttagtgaa aatgcgatga cggcgccgaa
      601 ccctttcatt tgccgcagcg ccagttcatg ttgcggatgg ctttgcaggc cgggataaaa
      661 aacgcgctcg acttgcggtt gttcttcgag ccagtgcgca agcgcgagcg cattggcgtt
      721 gatccgctcc atgcgcagcg acagggttct aagcccgcgt aaaagcagcc atgcgtccat
      781 gggagacaac accgccccga gcgtgatgtg catgtgccat atgcgttcgg caatgctgtt
      841 cgagcagcac acagcgcccg ccgtgacatc gtggtgtccg ccgaagtact tggttgcgct
      901 atgcacgacg atgtcgatgc ccagtgcgtg cggtttttga tttattggcg aggcaaaggt
      961 gttgtctgca atagtggtga tgccgcgctc gcgcgccatt tcggccaccg cggccagatc
     1021 tgtcagcgac agagtcggat tgacgggcgt ttccaccatg atcagacgtg tttgcggccg
     1081 gatggccgcc ttcattgcgg aaagatcggt ttggtcgacg atgctgacct cgacgccgaa
     1141 tcgcggcagg acttcttcga aaagcttcga tgtgctcatg tagtggcgtt tctgcgcaat
     1201 gacatgatcg ccgcgcgaca ccaggcttag tacagttgtg ctgatggcgc ccatgcccga
     1261 ggccatcacc atcgccgttt ctgtgctctc aagctctgcc agaatagctt cgacccgttt
     1321 gtgaaccgga ttgccgtatc gcgtgtaata cgcggacgga cgcaccgtgt tggccttttc
     1381 gacgaagtcc tcggcgctct ccgcgcgaaa tgtcgcgcta tagtgaatgg gcgcaacgat
     1441 gctggagtcc gtcgacaacg ccgcgtcgcc atgcaaaacc agcgtttcgt cgcgccagtt
     1501 atttttatct ttcatgaatt cactccattg atgtttcggg cgccggacaa tgtttccagt
     1561 catggccggt gaatgcttca gtgtacgaaa attaccggaa gggcatttcg ttcagttcat
     1621 tttcagcgtt gtgacaattg atggatgagt tttttgagtt cggcttgatt taacgaaatt
     1681 agtggctcat tgccatgctg gacgcgtttc aattgctttc aattgacgtg aaaatttcca
     1741 gattgaatgc gaactaaagc cgggtcggcg attctataca cccatgagct gcaacgaggg
     1801 ttgtccgctc agcttgggag cataagcgct caagggaatt atttcggagc ttatctaaat
     1861 gatcacaaaa tcgcgtgttg cacttgcctt atccacttcg gctctgtgcc tgggcgttgc
     1921 attggcccct gcagctttcg cagcgggtac aacaactgca actcatgcta aaaccaaaca
     1981 tcacaaagtt caaaaaacga atgccaaggc tgcaactcgt gccccggccg ctaaagctaa
     2041 agtacaaccg gccacgcaac ctgcgaagta atttttcgcc cgtcttttag tgttcatttg
     2101 aaagccagac ccgttttttc gggtctggct tgttaatttt gttttccgtt gcgtaaggga
     2161 aaaccataga agaaactgct ttttgaggaa gtggcgcgca cttataatta tgaattatta
     2221 atacaaggcc tccaagcggg ggtatcgtct aatgagtgcc cgtattgtcc agcaagttct
     2281 gtatcttgat gtcgcggatc gtctgcgcgc catgattcag gcgcaagtgt tggcggcggg
     2341 cgcatggata gacgagacca agctcaccgc cgaactcggc atttcccgca cgccgttgcg
     2401 cgaagccttg aaggttctcg ccaacgaagg gctggtgcgg cttgagccgc gccgcggctg
     2461 ttttgtaaac gagctgtcgg cgcgcgacct ggaccagatt tttccgttga tggcaatgct
     2521 ggaagggcgc tgtgcgttcg aagccgctca aaaagcaagc gtttcggatt tgcgcaagct
     2581 tgaacctttg catatcgaat tgcaggccta tgcctcgctg gagcacattg atcagtatta
     2641 cgcgcttaat gcccgaattc acgaagcggt gcaggctttg gccgataacc gctggctatc
     2701 cagtttaatc gataatctgc gcaaggttct cagtttatcg cgtcaccaaa gcttggtggc
     2761 gccgggtcgc attcatgaat cctgcgcgga gcatctggcc attttcgcgg cgctcaaggc
     2821 gcgcgatgcg caagcagccg aggcattgac gcgcggacac ttgatgcacc agcttgacgc
     2881 gcttcatgta ttgctcaatc aggccgatcc ggatacgggc gcgcttgaat ccagttcgtc
     2941 cattggctat tcaggagccg atttatgacc gactcaggca cgcgggaacc cgaaagcaag
     3001 cccgccgaag cggacaggga aaatttgccg gtcgccgcca atttgctagc gcgtttgggg
     3061 cgctggtggg aacgcgagcg ttggccgggg ccagccgaaa ctaaaacttt gttttcagcg
     3121 cgcctgaccg acgtggcggc gaatcgcctg gctcagacct ggtgcgaacg ctacgg
//
LOCUS       DATCXU010000014         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_940858,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000014 DATCXU010000000
VERSION     DATCXU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940858"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIR04_00285"
     CDS             <1..217
                     /locus_tag="VIR04_00285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9459214.1"
                     /translation="IQAGVTGLLVPLHNRDALTQALKKLIDDPALRERMGQAGQQRVR
                     GERAFSSEAMACRLESCYTRWLNERRS"
     gene            214..1056
                     /locus_tag="VIR04_00290"
     CDS             214..1056
                     /locus_tag="VIR04_00290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY9459215.1"
                     /translation="MKAARAVPVLMYHHVSPSEGMITTSPVNFERQLAWLRRHGYRSL
                     TGDGFAKHLAGGPTPPRSILITFDDGYLDNWVYAYPLLKRYGFNAMVFLVTSWIGEGL
                     ARPCLGQGDLPETPSHRECEARIQRGARDEVMLRWAEVKTMLADGVFEFHSHTHSHTR
                     WDKSDQADHKNTLMAEELALSRKTLSEHLGRVSSHLCWPQGYFDADYIELARQAGFRY
                     LYTTNAFGQNRAGMDPAFIYRFAVRNTSRGSFGRRIWIAADPWFGPLFNQWKLWKRSR
                     RRQT"
     gene            1053..1811
                     /locus_tag="VIR04_00295"
     CDS             1053..1811
                     /locus_tag="VIR04_00295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY9459216.1"
                     /translation="MTLSVIIIVKNESRHIGECIDSVAFADEIIVVDSGSSDDTCAIA
                     RAKGALVTQTADWPGFGRQKNRALDLATKDWVLSVDADERVTPELAQEIQRTLQNPRA
                     DAYKISRLSNFQGRWIRHSGWWPDRVLRLFKRGAARFSNDIVHESVQSSRPAALLSAH
                     FLHYPYADMESLIAKMNRYSSDAASVMHDQGKTASPLGAAGHALWTFIRIYLVRRGFL
                     DGKEGFVLAVTAAAGNFFRYGKLMFLNRKPKERP"
     gene            1823..2530
                     /locus_tag="VIR04_00300"
     CDS             1823..2530
                     /locus_tag="VIR04_00300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY9459217.1"
                     /translation="MYHQIGPPAPKGSPYRGLTVHPADFRRQMVWMRRLGYCGLSMRD
                     LMPHLKAEQSGKVFGITFDDGYRNVFQNAAPVLAELGFTATNYCVARQIGGDNVWDAD
                     KGIPRSALMSRAEMQQWAAAGHEIGSHTLDHVHLPELSPELADYQIRQSRVELEQGLG
                     VEVNAFCYPYGHESPDIRAMVRDAGYLNATTTERGLARRSDDLYGIPRVAISRSTNLL
                     YFLQKCLTRLEDRRRGD"
     gene            2538..3665
                     /locus_tag="VIR04_00305"
     CDS             2538..3665
                     /locus_tag="VIR04_00305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY9459218.1"
                     /translation="MKIAFVVDRFGARFGGAESYGVALMRELARTCDITVFAREYDES
                     CDLRLPFVSLRSWSGWPSWIRVLLFAIRARRRTRHGFDIVHSHMNGWCGDIDVIHVTP
                     VRYNWRVRRLPFLKRALSRVSLRVQTYLRLEARRVAPRAGHRTVAVSHLIADQLRQAY
                     GPALSAPVIPPGVSVPDSRVCEQRQPMRSSLGWEEEDYVCLMVARNPLRKGLSTALEA
                     LFLLPARFKLLVVGADSTARDFVHKAHGGVLADRIRLISETSDVAPYYVAADLYVHPT
                     LNDSFGMAPLEAMSFGLPVVLSPEPWCGFAHYVQDKSEAMLLSHPEDATQLARFIGQI
                     SQDGALRTRLAQGGSQVVKRHGWTGIASQYRGLYEQVISER"
     gene            complement(3719..>3843)
                     /locus_tag="VIR04_00310"
     CDS             complement(3719..>3843)
                     /locus_tag="VIR04_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY9459219.1"
                     /translation="VVPNDPDFSFWLPDMRAFEASRSIIKEYVGLLVYWVRGWI"
BASE COUNT          758 a         1022 c         1170 g          893 t
ORIGIN      
        1 gatacaggcc ggcgtaaccg gcttgctggt gccattgcat aatcgggacg ccctcactca
       61 ggcgttgaaa aaattgatcg acgatcccgc gttgcgcgag cgaatgggtc aggcaggcca
      121 gcaacgggtt cgcggcgagc gtgccttcag ttccgaagcg atggcatgcc gcctggagtc
      181 ttgctacacc cgctggttaa acgagcgtcg gtcatgaaag cggcccgggc ggttcctgtg
      241 ttgatgtatc accatgtctc gccttctgaa ggcatgatca cgacctcgcc cgttaatttc
      301 gaacgccagc ttgcgtggct aaggcggcat gggtaccgat cgctgaccgg cgatggcttt
      361 gccaagcatt tggccggagg cccgacaccg ccccgatcaa ttttgatcac tttcgatgat
      421 ggttacctgg ataattgggt ttacgcttat ccattgttaa aaagatatgg ctttaatgcc
      481 atggtttttt tggtgacttc ctggataggc gagggcttgg cgcgtccttg tctggggcag
      541 ggcgatctgc ccgaaacacc gtctcatcgt gaatgcgagg cgcgcattca gcgcggcgcg
      601 cgcgacgaag tcatgctgcg ttgggccgag gtcaagacca tgttggccga cggcgtgttc
      661 gaattccaca gccacacgca ttcgcacacc cggtgggaca aaagtgatca ggccgatcac
      721 aaaaacacac tcatggccga agagttggcc ttgtcgcgta aaactttgtc agagcatctg
      781 ggcagagtgt cgtcgcactt atgctggccg caggggtatt tcgatgcgga ttatattgaa
      841 ctggccaggc aggcggggtt ccgttattta tacacaacga acgcgttcgg gcagaatcgc
      901 gcagggatgg atcctgcatt catttatcgc ttcgcagtgc gcaatacgtc ccggggatcg
      961 tttgggcgtc gtatctggat tgccgccgat ccgtggttcg gtccattgtt caatcaatgg
     1021 aagctctgga aacggtcacg gaggcgccag acatgacatt gtcggtcatc attatcgtca
     1081 agaacgaatc gcgccatata ggcgagtgca ttgattctgt ggctttcgcc gatgagatca
     1141 tcgttgtgga ttcgggcagc agcgatgata cctgcgccat tgcgcgggcg aagggcgctc
     1201 tggtcacgca aacggccgac tggcctggtt tcggccgtca aaaaaatcgc gcgctggatt
     1261 tagcgaccaa ggattgggtg ttgtccgtgg atgcggatga gcgcgtcacg ccggagcttg
     1321 cgcaagagat tcagcgcacc ctgcaaaatc cgcgtgccga cgcgtacaag atttcccgcc
     1381 tgtcgaattt tcaggggcgg tggatacgcc acagcggctg gtggcctgat cgcgtgttgc
     1441 gtttgtttaa gcgcggcgcc gcccgtttca gcaatgacat tgtgcatgaa agcgtgcaat
     1501 catcgcgtcc ggcggctctg ctttccgccc atttccttca ttatccgtat gccgatatgg
     1561 aaagcctgat cgccaaaatg aaccgatatt cttccgatgc cgcgtcagtg atgcacgatc
     1621 agggtaaaac ggcgagtccg ctgggcgctg ccgggcatgc tttatggacc tttatcagga
     1681 tctatcttgt tcgccgtggt tttctggacg gaaaggaagg tttcgtgctg gccgtgacag
     1741 cggcggccgg caactttttt cgttacggca aacttatgtt cttgaatcgc aaaccgaaag
     1801 agcggccatg attccgatcc tgatgtatca ccagataggg ccgcctgccc ccaagggtag
     1861 cccctatcgc ggtttgaccg tgcatccggc cgatttcagg cggcaaatgg tctggatgcg
     1921 ccgtcttggg tactgcggct tgtcgatgcg cgatctaatg ccccatttaa aagccgaaca
     1981 atcgggtaaa gtatttggca tcacgtttga cgacggttat cgcaatgttt ttcaaaacgc
     2041 cgcgcctgtg ctggccgagc ttggcttcac agccaccaat tattgcgtgg cgcgccagat
     2101 tggcggggac aatgtctggg acgcggacaa gggaattcct cgctccgcgc tgatgtccag
     2161 agccgagatg cagcaatggg cggcggccgg gcacgaaatt ggttcgcaca cgctcgacca
     2221 tgttcatttg ccggaacttt cgcccgagct ggccgattat cagatacggc aatccagggt
     2281 cgagctcgaa cagggactgg gcgttgaagt caacgcgttt tgctacccgt acgggcacga
     2341 gtctccggat atacgggcta tggtgcgcga cgcggggtat ttgaatgcaa cgacaaccga
     2401 gcgcggcctg gcccggcgtt cggatgacct gtacggtatt ccacgcgttg cgatttcacg
     2461 ctcgacgaat ttgctgtact ttttgcaaaa atgcctgacg cgccttgagg acagacgccg
     2521 tggcgattga aacaaggttg aaaattgcct ttgtggtgga tcgcttcggg gctcgtttcg
     2581 gcggggcgga atcctacggc gtggcgttga tgcgcgaact cgcgcgaacc tgcgatatca
     2641 cggtttttgc gcgggaatat gacgaatcgt gcgaccttcg cttgccgttt gtttccctgc
     2701 gctcatggtc aggctggccg agctggattc gggtgctgtt gtttgcaata cgcgccaggc
     2761 ggcgcacgcg ccacggtttt gatattgttc attcgcacat gaacggctgg tgcggcgata
     2821 ttgatgtaat tcacgtgacc cccgttcgtt ataactggcg ggttcgccgt ctgccttttc
     2881 taaagcgcgc gctttcccgg gtcagtctgc gcgtgcagac ctacctgcgc ctggaggcgc
     2941 ggcgcgttgc gccacgtgcc ggacatcgga cggtggccgt atcgcacctg attgcggatc
     3001 agctcagaca agcctacggg ccggcacttt ccgcgccggt tatccccccc ggcgtgtccg
     3061 tgcccgattc gcgggtatgt gaacaaaggc agccaatgcg cagcagcctg ggttgggagg
     3121 aggaggacta tgtgtgtttg atggtggcca gaaatccgct gcgcaaaggg ctgtcgaccg
     3181 ctctggaagc gctttttttg ttgccagcgc gcttcaagtt gcttgtggtc ggggccgatt
     3241 cgacggcacg tgatttcgtg cataaagcgc atggcggcgt tttggccgat cgcatacgcc
     3301 tgatttctga aacgtccgat gtggctcctt attacgtggc agccgattta tacgttcatc
     3361 cgaccttgaa tgacagcttt ggcatggcgc cgctggaggc gatgtcgttt gggctgccag
     3421 ttgttttaag cccggagccc tggtgcggct ttgcgcatta tgtacaggac aaatccgagg
     3481 cgatgctctt gtcgcatccg gaagacgcga cgcaattggc ccgctttata gggcaaatca
     3541 gccaggacgg agcattgcgg acccgccttg cgcaaggagg aagccaggta gtcaagcgcc
     3601 atggctggac cggaattgcg agccaatacc gtggcttgta tgagcaggtg ataagcgaac
     3661 gataggcgcg ccgcaccttg acgcgtatgg cagtgcgcgc cgattcatcc gaatcttttt
     3721 atatccagcc gcgaacccaa taaaccagaa gtcccacata ttctttgatg atcgagcggc
     3781 ttgcttcaaa agcgcgcata tcgggtagcc agaaagagaa atcgggatcg tttggcacaa
     3841 caa
//
LOCUS       DATCXU010000015         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_861847,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000015 DATCXU010000000
VERSION     DATCXU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861847"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1085)
                     /gene="rfbB"
                     /locus_tag="VIR04_00315"
     CDS             complement(18..1085)
                     /gene="rfbB"
                     /locus_tag="VIR04_00315"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY9459220.1"
                     /translation="MKILVTGGAGFIGSALVRHVIADTGDAVVNVDKLTYAGNLESLA
                     DVAGSSRYAFEQVDICDAGEVARVFAQHQPDSVMHLAAETHVDRSIDGPEAFIQTNVV
                     GTFTLLEAARRYWLALDDKRRTAFRFHHISTDEVYGDLRDPDAFFTESTPYAPSSPYS
                     ASKAGSDHLVRAWRRTYGLPTIITNCSNNYGPYHFPEKLIPLTILNALEGRPLPVYGK
                     GNQVRDWLYVEDHARALHRVVTAGAAGETFNIGGHNERQNIQVVETICELLDELRPLS
                     RAGGGRHSYKELITHVADRPGHDQRYAIDAGKIQRELLWSPKETFETGLRKTVQWYLD
                     NQPWCQRVQDGSYRRERLGLR"
     gene            complement(1089..2285)
                     /locus_tag="VIR04_00320"
     CDS             complement(1089..2285)
                     /locus_tag="VIR04_00320"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY9459221.1"
                     /translation="MTTLQQAYIVAAIRSPIGKAPRGALSHTRPDDLLARVIRGMLAR
                     VPSLDPAAIEDAIIGCAMPEGPQGLNVARIGVLLSGLPNSVPGVTVNRFCASGLTAIA
                     MAADRIRVGEADVLIAGGVESMSLVPTMGFSPTFSPDVFAHDENLGIAYGMGATAEQV
                     ATQWKVSREDQDAFALQSNQRAVRAQKAGEFTDEILPLDVVNRVPDLVTGQVVEQHKK
                     FTLDEGPRPDTSLQALARLRPVFAARGSVTAGNSSQTSDGAGALLVVSERALKTHKLT
                     PLARFVSFAVRGVPPEIMGIGPKEAIPRALKLAGLKQDQLGWIELNEAFAAQSLAVIR
                     DLNLNSEIVNPMGGAIALGHPLGATGAIRAATVIHALRRHNLSYGMLTMCVGTGMGAA
                     GIFEKV"
BASE COUNT          484 a          728 c          686 g          467 t
ORIGIN      
        1 ttcggctcgc cacgcggcta ccgtagaccc agtctttcac gccgatagct gccgtcttgc
       61 acgcgctggc accagggctg attatccagg taccactgga ccgttttacg aaggcccgtc
      121 tcgaaggttt ccttcggcga ccacagcaat tcccgctgaa ttttgccggc atcgatcgcg
      181 taacgctggt cgtgcccggg gcggtccgca acatgggtga ttaattcttt ataagaatga
      241 cggccgccgc cggcgcggct tagcggacga agctcgtcga gaagttcgca gatcgtctcc
      301 acgacttgaa tattttgccg ttcattgtgg ccgccaatgt taaatgtttc gcctgccgcc
      361 ccagcggtga cgacccggtg caacgcacgc gcatggtcct ccacatacaa ccagtcgcgc
      421 acctgattgc ctttgccgta aaccggcaac ggtctgcctt ccagggcatt aagaatcgtg
      481 agcgggatca gcttttccgg gaaatgatac gggccataat tgttcgagca attcgtgatg
      541 atggtcggca gaccataagt gcggcgccag gcgcgcacca ggtggtcgga acctgccttg
      601 ctggcggaat agggggaact gggcgcgtac ggcgtggatt ccgtgaagaa cgcgtcggga
      661 tcgcgcaaat cgccatatac ctcgtcggtc gaaatatgat ggaaccggaa agcggtcctg
      721 cgcttgtcgt cgagcgccag ccaatatcga cgagcggctt ccagcaaagt gaaggtgccg
      781 acaacattgg tctgaatgaa cgcttccggg ccatcgatgg agcgatccac gtgtgtttcg
      841 gccgccaggt gcatgaccga atccggctga tgctgcgcga acacgcgcgc cacttcaccc
      901 gcatcgcaga tatccacctg ctcgaaggca taacgactgc ttccggccac atcggccaac
      961 gattccaggt tgcccgcata ggtcagcttg tcgacattga ccaccgcatc gccggtatcg
     1021 gcaatgacat ggcgcacaag agccgatccg atgaacccgg caccgccggt gacaagaatt
     1081 ttcatgcatc aaaccttctc aaaaataccg gcggcgccca tgccggttcc gacacacatg
     1141 gtcagcatgc cgtagcttaa attatgacgg cgcaaggcgt gtatgactgt ggcggcgcga
     1201 attgctccag tggcaccaag gggatgaccc aaggcaatcg cgccccccat tggattgacg
     1261 atttcagagt tcagattcaa gtcgcggatg accgccagtg attgcgcggc aaaggcctcg
     1321 ttaagctcga tccagcccaa ttgatcttgc ttcagacccg ccagcttcag cgcgcgcgga
     1381 atcgcttcct tcggcccgat acccataatt tccggcggta cgccacgaac cgcgaacgat
     1441 acaaagcgag ccaggggcgt cagtttatgc gttttcaagg cgcgttccga aaccaccagc
     1501 aatgcgccgg cgccatccga cgtctgcgag ctgttgcccg ccgttacgct ccctctggcc
     1561 gcaaagacgg ggcgcagccg cgccagcgcc tgcaggctgg tatcgggccg ggggccctcg
     1621 tcaagagtga attttttatg ctgctcaacg acttgtccgg tgaccagatc gggaacacga
     1681 ttcaccacat ccagcggcag gatttcatcg gtgaattccc ccgctttctg ggcgcggacc
     1741 gcgcgctgat tcgactgcag cgcgaaagca tcctgatctt cgcgcgaaac cttccattgc
     1801 gtggccactt gttccgccgt ggcccccatg ccgtaggcta tgccaagatt ctcgtcgtgc
     1861 gcgaaaacat cgggactgaa cgtggggctg aatcccatgg tgggtacaag gctcatggac
     1921 tcgaccccgc cagcgatcaa aacgtcggcc tcgcccacgc gaattcgatc cgccgccatc
     1981 gcgatggccg tcaggcccga agcgcaaaag cggtttacgg ttacgcctgg caccgaattg
     2041 ggcaagcccg acaacaagac cccgatacgc gcgacattca ggccctgcgg gccttcgggc
     2101 atggcgcaac cgataatggc gtcttcgatg gcggcagggt ccagactggg gacccgcgcc
     2161 agcatgcccc gaatcacgcg cgccagcagg tcgtcggggc gcgtatgcga gagcgcgcca
     2221 cgcggcgctt tgccaatggg gctgcgaatg gccgcgacaa tataagcttg ttgcaaggtg
     2281 gtcatggtga attccttaat tacgcaccgg tttgcccgtt tttaaaacgc tctggatgcg
     2341 ctcctgcgtt tttggctcgg caagc
//
LOCUS       DATCXU010000016         6986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_940221,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000016 DATCXU010000000
VERSION     DATCXU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940221"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..318)
                     /locus_tag="VIR04_00325"
     CDS             complement(91..318)
                     /locus_tag="VIR04_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY9459222.1"
                     /translation="MKLGTLSRLAMVALFTVAISGCASWDNMSYRQKSAVTGAGLGGV
                     AGAVITGGSVWGTAGGAAIGGLLGDQVGKSK"
     gene            complement(401..967)
                     /locus_tag="VIR04_00330"
     CDS             complement(401..967)
                     /locus_tag="VIR04_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY9459223.1"
                     /translation="MPSLNRRDSSVLIVDMQVKLLPAIQGYGLLLKRVEALIRGAGLL
                     DVPVLATEHCADKIGATAPVLRPWIQSTMPKTHFDAAREPEFKGFLPPSRPYVLLVGA
                     EAHVCVLQTALGLLDQGLQPLVVADGVGSRHAADYELALARCAYHRIQVVNVEMALFE
                     WMESAHHPKFKDVLKLIKSGPSGVENVA"
     gene            complement(981..2654)
                     /gene="ettA"
                     /locus_tag="VIR04_00335"
     CDS             complement(981..2654)
                     /gene="ettA"
                     /locus_tag="VIR04_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY9459224.1"
                     /translation="MAQYVYTMNRVGKIVPPKRQILRDISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGLDHEIEGEAIPMPGLKIGYLPQEPVLDSSHTVRQSVEDGLGELFSARK
                     RLDEVYAAYSEPDADFDKLANEQAELEAVIAAAASSGADDIDLQMEIAADALRLPPWE
                     AVIGQLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESIEWLEQFLRKFPGTVVGV
                     THDRYFLDNVAEWILELDRGHGIPWKGNYSSWLEQKDNRLKHEEAAESARQRTIAKEL
                     EWVRQSPKGRQAKAKARLARFDELSSLEYQKRNETQEIFIPVAGRLGNEVIEFKDVSK
                     GYGDRLLIDSFHARIPAGAIVGIIGANGAGKSTLFKMITGQERPDSGTVNMGATVQIA
                     FVDQSREHLPDEKTVFDAISGGADLMTVGRFEMGSRAYLGRFNFKGSDQNKLVGKLSG
                     GERGRFHLAKTLIAGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVLVISHDRWFLD
                     RIATHILAFEGESQLVFFDGNYQEYEADKKRRLGEEGAKPKRLRFKALK"
     gene            2843..3361
                     /locus_tag="VIR04_00340"
     CDS             2843..3361
                     /locus_tag="VIR04_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459225.1"
                     /translation="MPTAPDPDLIFTAEELDLLGRTADALSAYMGKPVLAQVIDAAES
                     GFEWVTFAVPLTPQESEEGRVVVQIGGPGARIVGNQGGLAIANGEIYNCEYLWAIQRS
                     PIEGVRFIKLDQQGDEVAWTDDLREILPFALVDEPPPDDDGDDDDDDGHGGDDGNPRK
                     GGADQARIRSRK"
     gene            complement(3334..4260)
                     /locus_tag="VIR04_00345"
     CDS             complement(3334..4260)
                     /locus_tag="VIR04_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY9459226.1"
                     /translation="MSVWRHLFTVLTPSYNRAHTLERVYLSLCDQSFQDFEWVVVDDG
                     STDSTRELVSQWQQTARFPIRYFRQDNQHKKTAFNRGVKEAHGELIVALDSDDTLDTN
                     ALDTMAQVWNEIAPDERPKFVAVTGLCERPGGQIVGDMFPRDVFDSTALDMSFKYHIK
                     GEKFGCMRTDVLRQFPFPEDIPGFVPESLVWRAMARAGYLTRFVNQVFRVYYESADSL
                     SNQGKTSQQHALGLFLLAQDTIVECLPWFRYQPLSFFMSAARYTRFALHVRRAHQRIP
                     PDRVLKGVWSWLLVALMWPVGVMLYLRDLIRA"
     gene            complement(4372..4500)
                     /locus_tag="VIR04_00350"
     CDS             complement(4372..4500)
                     /locus_tag="VIR04_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459227.1"
                     /translation="MAEVKRTRRNTLERRCLGKAFKRLFINAPKGVFKMLEKVKRA"
     gene            4692..>6986
                     /locus_tag="VIR04_00355"
     CDS             4692..>6986
                     /locus_tag="VIR04_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY9459228.1"
                     /translation="MSSQIRIFGARQNNLKNLDVSIATGELLVLTGVSGSGKSSLAFD
                     TLYAEGQRRYVETFSPYARQFLDRMDRPQVDRIEGILPAIAIDQVNPVRNSRSTVGTM
                     TELNDHLKLLYARMGRLYCRCCGAQVQRDSAESIQQQLAQRTQAGDPRLVVTFPVVIP
                     ANFTEEEVRGFLDQQGYTRIHHEETADFTPSPSKAKAKGKRPVKSEKLRVLHVIQDRF
                     RLGNAETSRVMEALDAALKQGDGQLAIYVSDDAGQEQAVWRFSNRLHCAHCNIEYTTP
                     LPSTFSFNSPLGACETCRGFGRIIGIDYGLVVPDENKTLREGAIKPWQTNSYKECQTE
                     MEHYAAGAGIRLDTPWRQLSEPERQWVIGGTPDWKGGRDAWKTQWYGAQRFFDWMESR
                     AYKMHVRVLLSKYRSYTPCPACGGSRLKSDATLWRLGESVGSINDDYPRFMPTHVHWS
                     REQLNALPGLGIHDLMRLPIDRVRDLFTTLSFGAAMDAAISLLLTEVRIRLKFLCDVG
                     LGYLSLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDIHRIVEVMR
                     RLRANGNTLIVVEHDPQIMIAADRILDFGPGPGERGGQIVFDGTVDQLRNANTLTGNY
                     LGGRLRVEAPRPMPVTSSTPRLLLEGVKANNLNGISIAIPLGRLVCVTGVSGSGKSTL
                     IQDVLYPAILKQKGRPTEAPGEYSELLGAEQLADVVMVDQTPIGKTARSNPASYVGAF
                     DAIRKLFAQATLSKERGYTAGTFSF"
BASE COUNT         1603 a         2064 c         1863 g         1456 t
ORIGIN      
        1 agctgcctcc caccgcggcg aaaggatagc gaagccgggt tcgggcttta agccgccctg
       61 tacaagttca gggctgagcc cgcttacttt ttatttgctt ttgccgacct ggtcgcccaa
      121 aagcccgcct atcgctgctc cacccgccgt gccccaaacg ctaccgcctg tgataacagc
      181 gccggcaaca ccgcccagac cggcgcccgt caccgcgctt ttctgacgat aactcatgtt
      241 atcccatgac gcacaacccg aaattgcaac ggtaaacagt gcaaccatcg ccaatctgct
      301 caatgtcccc agtttcacac gtatcctcca gtcaacaatg ctatttacca tacccgattc
      361 aggcgccctg tggcaactgc ggcggactat ttccgcaaac tcatgcaacg ttttcaacac
      421 cgctcgggcc ggatttgatt aatttcagca catctttgaa tttcgggtga tgtgccgact
      481 ccatccattc gaacagcgcc atctcgacgt tgaccacctg aatccgatga taggcgcaac
      541 gcgccaacgc caattcgtaa tcggcggcat gtctggaacc gacgccatcg gccaccacta
      601 gaggttgcaa tccctgatcc agcaggccca gcgccgtttg cagcacacac acatgcgctt
      661 ccgcgccgac cagcagcaca taagggcgcg atggtggcaa aaagcccttg aattcgggct
      721 cgcgcgcggc atcgaaatgc gtttttggca tggtgctctg gatccagggc cgcaacacgg
      781 gagctgtagc gcctatcttg tcggcacagt gttcggttgc gagcaccggc acgtccagca
      841 aaccggcacc tcgaatcagc gcctcaactc gcttcagcaa gagcccatac ccctgaatag
      901 ccggcaacaa ctttacctgc atatcaacaa tcaatacgct tgaatccctt cgattcaaac
      961 ttggcatggc gctccccttt ttatttcagt gccttgaacc gcaggcgttt tggcttggcg
     1021 ccttcttcgc ccagacggcg ctttttatct gcttcgtatt cttgataatt gccatcgaaa
     1081 aacaccagtt gagactcgcc ttcgaacgca aggatatgcg ttgcaattct atccagaaac
     1141 caccggtcat gactgataac cagcacgctg ccggcgaatt ccagaagcgc atcttcgagc
     1201 gcgcgcagcg tttcaacatc cagatcgttg gacggctcat ccagcaacaa tacattgccg
     1261 cctgcaatca acgttttggc caaatgaaat cggccacgct cgccgccgga taatttgcca
     1321 accagcttgt tctgatccga gcctttaaaa ttaaaacgcc cgagataggc gcgcgatccc
     1381 atctcgaagc gacctaccgt cattaaatcg gcgccgcctg aaatcgcatc gaacactgtt
     1441 ttttcatcgg gcaaatgttc tcgcgactgg tcgacaaagg cgatttgaac ggtcgcgccc
     1501 atattgaccg tgccggaatc gggcctttcc tgacctgtga tcatcttaaa tagtgtcgac
     1561 ttacccgcgc cattggcgcc gataataccc acaatggcgc ccgccggaat tctggcgtgg
     1621 aagctgtcta tcagcagacg atcgccatag cccttgctga cgtccttgaa ttcgatgacc
     1681 tcgttgccca ggcgcccggc cacagggatg aaaatttctt gcgtttcatt gcgcttttga
     1741 tattccagcg acgacagttc gtcgaaccgg gccaggcggg ctttggcttt ggcctggcga
     1801 cccttggggc tttgacgcac ccattcgagc tctttggcaa tagtgcgttg gcgagccgat
     1861 tcggccgcct cctcgtgctt cagccggttg tctttctgct cgagccagga gctgtagttg
     1921 cccttccagg gaatcccatg cccgcggtca agttcaagaa tccattccgc cacgttgtcc
     1981 agaaaatagc gatcatgcgt gacgcccact accgtacccg gaaacttgcg cagaaactgt
     2041 tccagccatt cgatgctctc agcgtccaga tgattggttg gctcatccag aaggagcatg
     2101 tcaggtttgg acagcaataa gcggcacaga gccacgcggc gcttttcccc gcccgacagt
     2161 tgtccgatca cggcttccca gggaggcaac cgcaaggcat cggcggcgat ttccatctga
     2221 agatcaatgt cgtcggcgcc actggatgct gcagcggcaa taacggcctc aagctccgcc
     2281 tgctcgttgg ccagtttgtc gaagtcggca tcaggttcag aataagccgc gtagacttca
     2341 tcaaggcgtt tacgcgcgct aaacagctcg cccaggccat cctctaccga ctggcgaacg
     2401 gtatggctgg aatcgagtac gggctcctgc ggcaagtaac cgatttttag gccgggcatg
     2461 ggaatggcct cgccctcgat ttcgtgatcg aggccagcca taatgcgcag cagggtcgac
     2521 ttgccggcgc catttaaacc caggacgcca atttttgcgc cagggaagaa agacaaggat
     2581 atatcgcgca aaatttgccg ctttggcggc acgattttcc caacgcggtt catcgtgtaa
     2641 acgtattgag ccatagaccg caaaaccaga aagtggtgaa agcctgattg tagcaacggc
     2701 gcgactgcgg ccatgttcgt tcttatgtgg cagcgccgcg caagttgaag ccagtgatct
     2761 ctcttaaatc catcaccccg cgcaagtcgg agcccattgc cagttaaaat actcacatcg
     2821 ctacttcaag ccgcaccaac ccatgccgac cgcacctgac cctgatctca ttttcacggc
     2881 tgaagaactc gacttgctgg gccgcaccgc cgacgcgctt agcgcctata tgggtaaacc
     2941 cgtactggcc caggtaatag atgcggccga aagcggcttc gagtgggtta ctttcgccgt
     3001 gcctttgact ccacaggagt cggaagaagg ccgggttgtg gtgcagatcg gcgggcctgg
     3061 cgcgcgcatc gtgggcaatc agggcgggct tgccattgcg aacggtgaaa tctacaactg
     3121 cgagtatctg tgggccattc agcgatcgcc catagaaggc gtgcgcttca tcaagcttga
     3181 ccagcagggc gacgaagtcg cctggaccga tgacttgcgt gaaattttgc cttttgcctt
     3241 ggtcgacgag ccgccgcccg atgacgacgg cgatgacgat gatgacgatg gccatggcgg
     3301 ggacgacggc aatccccgca aaggcggcgc cgatcaggcg cggatcagat cccgcaaata
     3361 aagcatcacg cccaccggcc acatcaaggc caccagcagc caggaccata ccccctttaa
     3421 tacgcggtcg ggaggaatgc gctgatgcgc ccgccttacg tgcaacgcga accgggtgta
     3481 gcgggcggcc gacatgaaaa acgataaggg ctggtagcgg aaccagggca agcattcgac
     3541 gattgtgtcc tgggccagta aaaacaggcc caaggcatgt tgctggcttg ttttaccctg
     3601 attggaaagc gaatcggcac tttcgtaata aacgcgaaac acctgattta caaaacgggt
     3661 caaatagccc gcgcgcgcca tggcgcgcca caccaggctt tcgggtacaa agccgggaat
     3721 gtcctcgggg aaagggaact ggcgcagcac gtcggttcgc atgcacccga acttctcgcc
     3781 tttaatatga tacttaaagg acatgtcgag cgcggtcgag tcgaagacgt cgcgcggaaa
     3841 catatcgccc acgatttggc ctccgggacg ttcacacaaa cccgtaaccg cgacaaactt
     3901 gggtctttcg tcaggcgcaa tctcgttcca gacctgcgcc atggtgtcaa gcgcgttggt
     3961 gtccagcgta tcgtcgctgt caagcgcaac gatcaattcg ccgtgcgcct cttttacgcc
     4021 gcgattgaac gcggttttct tgtgctggtt gtcctgtcga aaatagcgga ttggaaaacg
     4081 ggctgtctgt tgccactgcg agaccagttc cctggtagag tcggtcgagc catcgtcaac
     4141 gacaacccat tcaaagtcct ggaaggattg atcgcacaat gacagatata ctcgttcaag
     4201 cgtatgggcc cggttataag agggcgtcaa gacggtgaat aaatggcgcc agacagacat
     4261 gattgtttta tttataagcc gatatacgcc atgataaggc cccaaaaaaa aaccacaaca
     4321 tgaaattgtg gtttttttgc ttcgatgatg ccagaacgag aacgtggcac gttacgcgcg
     4381 tttcaccttc tcgagcatct tgaacacgcc cttgggcgcg tttataaaga ggcgtttgaa
     4441 agccttgccc aaacaacgac gctccagagt attgcggcgg gtccgtttaa cttctgccat
     4501 agtgatctct tccttttgtg cgacgaagca tggccaagca gccgcccgtc aaaaacacgt
     4561 taaacgcttc gaaacaaagc gttaatgttc aatccagtta tccaatgctg catattttag
     4621 cgcaaaaaca gtcgttccgc caaaagacag ggcgtcaaaa cagggccttg aagaacgact
     4681 atcatagcgt catgagttca caaatccgca tttttggcgc gcgccagaac aatctcaaga
     4741 accttgatgt ttccattgcc accggcgaac tattggtcct gaccggcgtg tccggatcgg
     4801 gtaaaagctc gctggcgttc gatacgctgt atgccgaggg ccaacgccgt tacgtcgaaa
     4861 cattctcacc atacgcgcgt cagtttctgg accgtatgga caggccgcag gttgaccgca
     4921 tcgaggggat tctgccggcc attgccattg atcaggtgaa tccggtacgg aactcgcgca
     4981 gcacagtcgg cacgatgacc gaactcaatg accatcttaa acttttgtac gcccgcatgg
     5041 gcaggctata ttgccgctgt tgcggcgcgc aggtgcagcg tgacagcgcg gaatcgatcc
     5101 agcaacaatt ggcgcaacgg actcaggccg gcgatccacg gcttgtcgtc acatttccgg
     5161 tcgttatccc ggcaaatttc accgaggagg aagtgcgggg gtttctggat cagcagggct
     5221 acacgcgaat ccatcacgaa gaaactgccg atttcacgcc ctcgccgtcc aaggccaagg
     5281 ccaagggaaa acgccctgtc aagtccgaaa agctgcgcgt cctgcatgtt attcaagacc
     5341 gctttcgcct cggcaacgcc gaaaccagcc gggtcatgga agcgcttgac gcagccctga
     5401 aacaaggcga cggacaactg gccatttatg tatcagacga tgccggccag gaacaagcgg
     5461 tgtggcgctt cagcaatagg ttgcattgtg ctcactgcaa catcgaatac accacgccac
     5521 tgcccagtac attttcattt aattcgcctc tgggcgcgtg cgaaacctgc cgcgggttcg
     5581 gccgaatcat cgggatcgac tatgggctgg tcgttccaga cgaaaacaag actctgcgcg
     5641 aaggcgccat caagccgtgg cagacgaaca gctataaaga atgccagacc gaaatggagc
     5701 actacgccgc cggcgcggga atacggctcg ataccccctg gcgccaactg agcgaaccag
     5761 aaaggcaatg ggtcatcggg ggtacgcccg actggaaggg cggacgcgac gcgtggaaaa
     5821 cccagtggta tggggcgcag cggttttttg actggatgga gtcgcgcgca tacaaaatgc
     5881 atgtgcgcgt gctgctctcg aaataccgca gttacacacc ctgcccggca tgcggcggct
     5941 ctcgtctcaa atctgacgcc acactgtggc gactgggcga atccgtcggc tcgatcaacg
     6001 acgactaccc gcgcttcatg ccgactcacg tccactggag ccgggaacaa ctcaatgcac
     6061 tgccgggttt gggtatccac gacctgatgc gcctgcctat cgaccgggtg cgcgatttgt
     6121 tcaccacact aagctttggc gcagccatgg atgccgccat cagtcttttg ctaaccgagg
     6181 ttcgaatccg cttgaaattt ctgtgcgatg tcggccttgg atatttatcg ctggatcgtc
     6241 aaagccgcac cctgtccggc ggcgaggttc aacgcataaa cctgaccacc gccctgggga
     6301 catcgttagt caacaccttg ttcgtcctgg acgagccgtc aataggcctg cacccccgcg
     6361 atattcatcg gatcgtcgaa gtcatgcgcc ggctcagggc caatggcaat acactgatcg
     6421 tggtcgagca cgacccccaa atcatgattg ccgcggaccg gattctggat ttcggacccg
     6481 gaccaggcga acgaggcggg caaatcgtct ttgatggcac agtcgaccag cttcgcaacg
     6541 ccaacaccct gacaggaaat tatctgggcg gccgtctgcg tgtcgaagcg ccccgcccga
     6601 tgccggtaac gtcaagcaca ccaaggctgt tgctcgaagg cgttaaagcc aacaatctaa
     6661 acggcatttc aatcgccatt cccctgggcc ggctggtctg cgttacggga gtgtcgggat
     6721 caggcaagtc aaccctaata caagacgtgc tgtatccggc gattcttaaa caaaagggca
     6781 ggcccaccga ggcgccgggc gaatacagcg agcttttggg cgcggagcaa ctggccgacg
     6841 tggtcatggt tgatcagacc cctattggaa aaacagcgag gtcaaatccc gccagttacg
     6901 tcggcgcctt cgatgccatc cgaaagcttt ttgcgcaagc gaccctttcg aaagagcgcg
     6961 gctacacagc cggcacattc agcttc
//
LOCUS       DATCXU010000017         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_153242,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000017 DATCXU010000000
VERSION     DATCXU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153242"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="VIR04_00360"
     CDS             <1..2087
                     /locus_tag="VIR04_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEY9459229.1"
                     /translation="KLTPELQSEINAADTKQRLEDLYAPYKPRRRTRAQIAREAGLEP
                     LADAILNEPLCDPASLAARYVNIDTSIADSKAALDGARDILSERYAENADLLTQLRTY
                     LWNSGRLYSSVAKGKENEGSNFRDWFDFNEPLRSLPSHRILALLRGRQQAVLDLRLGL
                     DPALEAQTPHPCIARIADYLQIGARFEPDASPRARWLGEVCRWTWRVKLLATFETELI
                     GRLRDSAEQEAIRVFAANMKDLLLAAPAGPKSVIGLDPGIRTGVKAAVIDATGKLLDT
                     ATIYPFEPRRDWEGSIRTLAALALKHKVELAAIGNGTASRETEKLVDDMLGRFPDLNL
                     TRVVVSEAGASVYSASELAAHEFPDLDVTLRGAVSIARRLQDPLAELVKIEPKAIGVG
                     QYQHDVNQRELAHSLDAVIEDCVNAVGVDVNTASAPLLARVSGLNSTLAKNMVAWRDE
                     NGAFKDRKTFLKVARFGDKAFEQAAGFLRIPGADNPLDASAVHPEAYPVVQRILKKIN
                     TDARQIMGRQGALKGISPSEFTDDRFGLPTVQDIFTELEKPGRDPRPEFKTAQFKDGV
                     NTLADLRAGMILEGAVTNVANFGAFIDIGVHQDGLVHISALADKYVKDPREVVRVGQT
                     VKVKVQEVDIARKRIALTMRLNETIAPPRRDNDHRSNDRQRTGPAAASARRPSQTPAT
                     GAMAAAFAKLKK"
     gene            complement(2112..3464)
                     /locus_tag="VIR04_00365"
     CDS             complement(2112..3464)
                     /locus_tag="VIR04_00365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY9459230.1"
                     /translation="MNTRVLTGITTSGTPHLGNYAGAIRPSILASNRPDVDAFFFLAD
                     YHALIKCDDPQRIARSRLEIAATWIAAGLDTDRATFYRQSDIPEIPELCWMLTCVTAK
                     GLMNRAHAYKAMVDQNVEKQTDPDDGVTMGLFSYPILMAADILMFKANKVPVGRDQIQ
                     HIEMARDIAQRFNHLYGNGTDFFVLPEAQIDESVATLPGLDGRKMSKSYNNTIPLFEG
                     GAKALCAAVMRIVTDSRLPGEPKDAEASHVYTLYKAFATPEQSSAFRQQLEDGLGWGD
                     AKKRLCDRIEQDLAPMRERYAELMAAPQRIEEILQAGAAKARSLSSVFMAQLRDAVGI
                     RQSGTRHAAAKHSTVDADLKSGAGKLARFVSFRDDHGQFRFRLLDSDGRELLLSEPFS
                     DPKASGLAIRRLKSSPLDGLSVEQEGVAVLKIGDTVISRFESAKLALVQAALAGFDRN
                     "
     gene            complement(3533..4624)
                     /gene="zapE"
                     /locus_tag="VIR04_00370"
     CDS             complement(3533..4624)
                     /gene="zapE"
                     /locus_tag="VIR04_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY9459231.1"
                     /translation="MNVLEYYEQALIDRDFRPDRAQLAAAQRLQRYFDEWVEFKRARS
                     TKLKKMFKRPDIPRGIYLWGGVGRGKSFLMDAFYSTVPVVRKTRLHFHEFMRGIHREL
                     FAVRGQSDPLDEVARRIAKRYRLICFDEFHVSDVADAMILYRLLLKLFEYGVSFVMTS
                     NYEPSTLYPDGLQRDRILPAIALLEERMDILNVDTGVDYRRRNLERVRMYITPITPQV
                     NEELRSIFTRLSDTAPQKPVLKIANRAIKAIAVSGSIVWFDFATLCGGPRSQNDYLDL
                     AARFHTVILSDVPRLGPRQASAARRFTWLVDVLYDHKIKLIISAQCEPDELYKAGPMV
                     NDFYRTASRIMEMQSREYLQSERREAAVL"
     gene            complement(4635..6002)
                     /gene="lpdA"
                     /locus_tag="VIR04_00375"
     CDS             complement(4635..6002)
                     /gene="lpdA"
                     /locus_tag="VIR04_00375"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY9459232.1"
                     /translation="MRAAQLGMSVACIDAWKTADGKPALGGTCTNIGCIPSKALLQSS
                     ENYDQANHHFAAHGIEIKGISVKLETLLGRKNTVVKQNNDGILYLFKKNKVTFFHGVG
                     SFVARVDGGWSIKVSGSTEDELVARHVIVATGSTARAWSGLPFDEAQILSNDGALSIP
                     AVPKKLGVIGAGVVGLEMGSVWSRLGAEVTLLEAMPEFLAAVDQQTAKEALKALTKQG
                     LVVHTGVKISDIKSSPKSVAVTYTDSAGAEQKLVVDKLIISIGRVPNTQGLAAQTVDL
                     KLDERGFVAVDADCKTNLPDVWAIGDVVRGPMLAHKAEEEGVAVAERIAGQQGHVNFG
                     TIPWVIYTSPEIAWVGKSEQQLKAEGIDYKAGSFPFAANGRARALGETTGFVKVLADA
                     KTDEVLGVHIIGPTASELISEAVTIMEFRGAAEDIARICHAHPTLSEAVKEAALAVDK
                     RALNF"
BASE COUNT         1375 a         1775 c         1633 g         1221 t
ORIGIN      
        1 acaaactcac tcccgagctg caaagcgaaa ttaacgcggc cgacaccaag caacggctgg
       61 aagatttgta cgcgccctac aaaccacgac gccgcactcg cgcacagatt gcgcgtgaag
      121 cgggtctgga gccactggct gacgctattt tgaatgaacc gctatgcgac cccgcgagcc
      181 tggccgcgcg ctatgtcaat atcgacacct cgatcgccga ttcgaaagcc gcgcttgacg
      241 gcgcgcgcga catcctgtcc gagcgctatg cggaaaatgc cgaccttttg acgcaacttc
      301 gcacctactt gtggaattca ggccgccttt attcatcggt cgccaaaggc aaagaaaacg
      361 aaggaagcaa ctttcgcgac tggttcgatt tcaacgagcc gttgcgctcg ctgccctcgc
      421 accggatcct ggcattgttg cgcggacgcc agcaagccgt gctggatctg cggttggggc
      481 tggaccccgc gctggaggcg caaacgcccc atccgtgcat cgcgcgcatc gcggattatc
      541 tgcaaattgg cgcgcgcttt gaacccgacg catcgccacg cgcacgctgg ctgggcgaag
      601 tctgccgctg gacctggcgc gtcaagctgc tcgccacctt cgaaaccgaa ttgatcgggc
      661 gtttgcgcga cagcgccgaa caggaagcca tacgcgtttt cgcggccaac atgaaagact
      721 tgctgctggc ggcgcctgca ggccccaaat cggtcattgg cctggatccg ggcattcgca
      781 ccggcgtcaa ggcagccgtg atcgacgcca ccggcaaatt gctggacacc gccaccattt
      841 acccattcga acctcggcgc gattgggaag gatccatacg cacgctggcg gcactggcgc
      901 taaagcataa agtcgaactg gcggccatag gcaatggaac cgcatcgcgc gaaaccgaga
      961 aactggtcga tgatatgctc ggcaggttcc cggacttgaa tttgacgcgc gtcgtggttt
     1021 ccgaggccgg tgcttcggtc tactcggcct ccgaactggc ggcgcacgaa tttcccgatc
     1081 tggatgtcac actgcgcggc gccgtatcga tcgcgcgccg tttgcaggac cctctggccg
     1141 agctggtgaa aatcgaaccc aaggccatcg gcgtggggca atatcagcac gacgttaatc
     1201 aaagagagct ggcgcattcg ctggatgccg taatcgagga ctgcgtgaac gccgtgggcg
     1261 tcgacgtcaa tacagcgtcg gcgccgttgc ttgcacgtgt atccggtctc aattccaccc
     1321 tggctaaaaa tatggtggcc tggcgcgatg aaaatggcgc attcaaggat cgtaaaacgt
     1381 tccttaaagt tgcccgattc ggcgataaag cgttcgaaca ggctgcgggt ttcctacgta
     1441 ttccgggcgc cgacaacccg ctggatgcgt cggcggtgca ccctgaagct tatccagtcg
     1501 tccagcgtat tttaaaaaaa atcaacacag acgcgcggca aatcatgggg cggcaagggg
     1561 cgttaaaagg aatatcgccc tccgaattca cggacgatcg tttcggcctg ccgaccgtgc
     1621 aagacatatt caccgaattg gaaaaaccgg gtcgcgaccc tcgaccggaa ttcaaaaccg
     1681 ctcaattcaa agacggtgtc aatacacttg cggacctgcg tgcgggaatg atcctggaag
     1741 gcgccgtcac caacgtggca aatttcggcg cttttattga catcggcgtg caccaggacg
     1801 gacttgtcca tatttccgcg ctggccgaca agtatgtaaa agacccacgc gaagttgtgc
     1861 gagtggggca aaccgtcaag gtgaaagtgc aagaagtgga tatcgcacgc aagcgcattg
     1921 cgctgaccat gcgcttgaac gaaactattg cgccgccgcg ccgggataac gaccaccgaa
     1981 gcaacgacag gcaacgcacc ggcccggcag cggccagcgc caggcgcccc tctcaaactc
     2041 ccgccaccgg tgcaatggcg gccgcctttg ccaaacttaa aaaatgaatc gtggcgcttt
     2101 ccaaagcgcc atcaattccg atcaaaaccg gccagcgctg cttgcaccaa agcaagtttg
     2161 gcggattcga accggcttat gacggtatca ccaatcttga gaaccgctac accttcctgt
     2221 tcgaccgaaa ggccatccag cggcgagctc ttcagacgac ggatagccag accgcttgcc
     2281 ttgggatcgc tgaatggttc tgacagcagc agttcgcgcc catcagaatc cagcagacga
     2341 aagcggaact ggccgtgatc atcacgaaag ctgacaaatc gcgccagctt gccagcccca
     2401 ctctttaaat cggcatcgac ggttgaatgt ttcgccgccg catgtcgcgt tccgctctgg
     2461 cgaatgccta ccgcgtcgcg caattgcgcc ataaacacgc ttgataaaga gcgagccttg
     2521 gcggcgccgg cttgcaggat ttcttcaata cgctgagggg ccgccatcaa ttccgcatag
     2581 cgttcgcgca tgggcgccag atcctgttcg atacggtcgc acaggcgttt tttcgcatcg
     2641 ccccagccca agccgtcttc gagctgttga cgaaatgccg acgattgctc cggcgtcgca
     2701 aacgccttat ataacgtata aacatgcgag gcctcggcat cttttggctc tccgggcagg
     2761 cgggaatcgg taacaatccg catgaccgcc gcgcacaggg ccttcgcccc gccctcgaac
     2821 aaggggatcg tattgttgta gctcttggac atttttcggc catcgaggcc tggcaaagta
     2881 gccacggatt catcaatctg agcttcgggc agtacaaaaa aatcagtgcc gttgccatag
     2941 agatgattga atcgctgagc gatatcgcgg gccatttcaa tatgctgaat ctgatcgcgt
     3001 cccacgggga ccttattggc tttaaacata aggatgtcag ccgccatcaa gatggggtat
     3061 gaaaacagac ccatcgtgac gccgtcgtcg gggtccgtct gtttctccac gttttgatcg
     3121 accatggcct tgtacgcatg ggcgcgattc atcaggccct tggccgtcac acacgttagc
     3181 atccagcaca attcgggaat ttcagggatg tcggactggc gataaaatgt agctctgtcg
     3241 gtatccaggc cagcggcaat ccaggtggct gcgatttcaa gacgcgaacg cgcaatgcgt
     3301 tgcgggtcgt cgcatttgat cagagcatga taatccgcca aaaagaaaaa ggcatcgaca
     3361 tccggccgat tgctggcaag aatcgacgga cggatagctc cggcgtaatt acccaaatga
     3421 ggggtgcccg aagtggtaat gccggtcagc acgcgcgtat tcatagctca gttttcaaaa
     3481 atggttaaac aggccatcat gcttcgatgg cgcgcgacaa cgattgccgg tattacaaga
     3541 cagccgcctc gcgacgctct gactgaaggt attcacgcga ctgcatttcc ataatgcgcg
     3601 aggcagttcg ataaaaatcg ttgaccatgg ggcccgcctt gtaaagttcg tcgggctcgc
     3661 actgggccga aataatcagc ttgattttgt gatcgtaaag cacatcgacc agccatgtga
     3721 accggcgcgc ggcagacgct tgacgcggtc ccagacgcgg cacgtcggac aaaataaccg
     3781 tatgaaagcg ggccgccaaa tcgagataat cattttgtga ccgcgggccg ccgcataacg
     3841 tcgcgaaatc aaaccacacg atgctgccgc ttacagcgat ggcttttatc gcacgatttg
     3901 caatttttaa gaccggtttt tgcggcgcgg tgtccgataa gcgcgtaaaa atggatcgca
     3961 gctcttcgtt tacctgaggg gtgatgggcg ttatgtacat tcgaacacgt tccagattcc
     4021 ggcggcgata gtccaccccc gtatccacgt tcaaaatatc catgcgctct tcaagaagcg
     4081 cgatcgccgg tagaatccga tcgcgctgca aaccgtcggg atacaaagtc gacggctcgt
     4141 aattggacgt catgacaaac gagacgccat attcaaacaa cttaagcagc aagcgataaa
     4201 gaatcatcgc gtccgcgacg tcggaaacat gaaattcgtc aaagcaaata agccggtaac
     4261 gtttcgcgat gcgtctggct acctcgtcca gagggtcgga ctggccccga accgcgaaga
     4321 gctcgcgatg aatgccacgc atgaactcat gaaaatgcag tcgtgttttt cgcacgacgg
     4381 gcacggtcga gtagaaagcg tccatcagga aacttttgcc gcgtcccaca ccgccccata
     4441 agtaaatgcc gcgagggatg tcggggcgct tgaacatctt cttcagtttc gtcgaccggg
     4501 cccgcttgaa ttcgacccat tcatcgaaat agcgctgcag ccgctgcgcg gcggccaact
     4561 gcgcccgatc aggccgaaaa tctcgatcga tgagcgcttg ctcgtaatac tctagaacat
     4621 tcattaacgg cgaatcagaa attcaacgcg cgcttgtcca cggccaaagc cgcctctttg
     4681 acggcctcgg acaacgtcgg atgcgcatgg caaatacgcg cgatgtcctc ggcggcgccg
     4741 cggaattcca tgattgtgac cgcttctgaa ataagctcgg acgcggtcgg gccgataatg
     4801 tgcaccccca atacttcgtc cgtcttggcg tcggcaagca ccttgacaaa acctgttgtc
     4861 tcacccaagg cgcgggcccg gccattggcg gcgaagggaa agctgcccgc cttgtaatcg
     4921 atgccctcgg ctttaagctg ctgttcgctt tttccgaccc aggcaatttc aggcgatgtg
     4981 taaataaccc acggaatggt tccaaaattc acatgccctt gctgtccggc gatacgttcg
     5041 gcgacggcca cgccttcttc ttcggccttg tgagccaaca tggggccacg aaccacatcg
     5101 cctattgccc agacatcggg caaattagtt ttgcaatcgg catccacagc cacaaaaccg
     5161 cgctcgtcga gcttcagatc gaccgtctgc gcagccaacc cctgagtatt tggaacacgc
     5221 ccaatcgata tgatcagttt gtcgaccacc agcttctgtt cggctccggc cgaatcagta
     5281 taggtcacgg caaccgactt gggactggac ttgatgtcgc tgatttttac cccggtatgc
     5341 accaccagcc cttgtttggt cagcgccttg agcgcctctt tagccgtttg ctgatcaaca
     5401 gccgccagaa attcgggcat ggcttccagc agcgtcactt cggcgcccag ccggctccag
     5461 acgctgccca tttccaggcc cacaacgccc gcgccgataa cgcccagttt tttaggcacc
     5521 gcgggaatgc taagtgcacc gtcgttggaa aggatctgcg cttcatcgaa aggcaatccc
     5581 gaccaggcgc gcgccgtgga gcctgtggca acgatcacat gcctggccac caattcgtcc
     5641 tcagtcgagc cggacacctt gatcgaccag ccgccgtcga cgcgcgcgac gaacgaaccc
     5701 acgccgtgaa agaaagtaac cttgtttttc ttgaacaaat acaaaatgcc gtcgttgttt
     5761 tgcttgacaa cggtattctt gcggccaagc aaggtttcaa gtttgaccga gatccccttg
     5821 atctcgatgc catgcgcggc aaaatgatga ttggcctgat cgtaattttc ggacgactgc
     5881 aacagcgcct tggacggaat gcagccgatg tttgtgcacg tgccgcccag agccggtttg
     5941 ccatcggccg tcttccaggc gtcgatgcat gctaccgaca ttcccaactg ggcggcccga
     6001 atag
//
LOCUS       DATCXU010000018         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1090538,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000018 DATCXU010000000
VERSION     DATCXU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090538"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /gene="araD"
                     /locus_tag="VIR04_00380"
     CDS             complement(<1..1667)
                     /gene="araD"
                     /locus_tag="VIR04_00380"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEY9459233.1"
                     /translation="MRTPESLRSARWFAPDDLRGFGHRSRVMQMGYAPEDWVGKPVVG
                     IINTWSDIQPCHAHFKTRVEDVKRGILQAGGFPIELPAQSLAEMYVKPTTMLYRNMLA
                     MNTEELLRCHPIDGVVLMGGCDKTTPALLMGATSAGLPAIYIPAGPMLRGNWEGHVLG
                     SGSDSWKYWDERRAGNITQNQWLGIEGGIARSYGTCMTMGTASTMTAVAESLGMSLSG
                     ASSIPAADSNHIRMCAASGRRIVEMIWEDVTPDKIQTQAAFQNAITVAMAMGCSTNAI
                     IHIIAQAKRANCDISLEDFERASRKIPVMANVRPSGDKYLMEDFYYAGGLRGLMSQLK
                     DHLDLSVLTVTGKTLGENLEDAAVYNDDVIRKFDNPIYAEGSLAVLKGNLAPNGCVIK
                     PAACEKRLLKHTGKALVFDSYPEMKEQINRPDLEVTADDVLILRNVGPQGGPGMPEWG
                     MIPIPQKLIEKGVRDMVRISDARMSGTSYGACVLHVSPEAYIGGPLGLVENGDEISID
                     VPGRSIHLNVSEKELAARKARYKAPPARFERGYGWMFAQHIQQANEG"
     gene            complement(1691..2560)
                     /locus_tag="VIR04_00385"
     CDS             complement(1691..2560)
                     /locus_tag="VIR04_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459234.1"
                     /translation="MSRAIITLKQDTVPASETQKKVIVARKLSSLEKLWSDAFVRKAT
                     IILLLAIAWEVYGRLLGNPLLFPTLSDTFQSFVDRIGDGILLDRAWTSIRVLLIGYSL
                     GIVIAAIMTILAISTQIGTDFLEVMTAMFNPLPAIALLPLALIWFGLGTTSIVFVLLH
                     SVIWVVALNTQAGFLAVSHTLRMVGRNYELRGLRYVALILIPAAFPSILTGLKIGWAF
                     AWRTLIAAELVFGVASGKGGLGWFIFENRNLLDIPAVFAGLLTVIIIGLIVENLVFRL
                     IEKKTVRRWGMQS"
     gene            complement(2560..3324)
                     /locus_tag="VIR04_00390"
     CDS             complement(2560..3324)
                     /locus_tag="VIR04_00390"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459235.1"
                     /translation="MPCLLEVDAVTLRYKTPDSIVTATDKVSFDVNSGDRFVLLGPSG
                     CGKSTLLKAVGGYITPDEGEIRIKGSVVQRPGSDRMMVFQEFDQLLPWKTVLENVMFP
                     LAVTQRLPKKEAEERARAYVEKVHLTQFANSYTHTLSGGMKQRVAIARGMSMEPDILL
                     MDEPFAALDALTRRQMQDELLQLWHETQFTVLFVTHSIAEAIRIGNRILLLSPHPGEV
                     KAEVSNIDAVSSDNVLAAELEQEIHDMLFNQEGAHP"
     gene            complement(3365..>3907)
                     /locus_tag="VIR04_00395"
     CDS             complement(3365..>3907)
                     /locus_tag="VIR04_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459236.1"
                     /translation="SEKEFGKDQWSKLDANTVQLGHPDAAAMLANPKGEISTHFAAPP
                     YDYKELKTVPGAHVVLRSTDIIDGGLSQSTLFTTTDFAKSNPKIIKAVLDASKEAIDY
                     IKANTEKSIEIYREISGDKTSVADLMEMLKQPGMMAFQMAPAGSMKFAEHMHKVGILK
                     TMPKNWTDYFLPASSGLNGS"
BASE COUNT          800 a         1085 c         1112 g          910 t
ORIGIN      
        1 caaccttcgt tcgcctgttg aatgtgctgc gcgaacatcc agccgtaacc gcgttcgaat
       61 cgagctggcg gcgctttgta tctggctttc ctggcggcca gttccttttc cgaaacgttc
      121 agatggatgg atcgcccggg aacgtcaatc gaaatttcat cgccgttttc caccaggccc
      181 agcgggccac ctatataggc ctcgggcgat acgtgcaaaa cgcaggcgcc gtagctcgtg
      241 ccgctcatgc gggcatctga aatgcgcacc atatcgcgca cgcctttttc gatcaatttt
      301 tgcggaatcg ggatcatgcc ccactcgggc atgccggggc cgccttgcgg accgacgttg
      361 cgcaggatca ggacatcgtc ggcggtcacc tccaggtcgg gccggttgat ttgttctttc
      421 atttccgggt agctgtcaaa gaccagcgct ttgcccgtgt gcttcagcaa gcgcttttcg
      481 caggcggccg gtttgatcac gcaaccattg ggcgccagat tgcctttgag caccgcgagc
      541 gagccttcgg cataaatcgg attgtcgaat tttcggatca catcgtcgtt gtagacggcg
      601 gcatcttcca ggttttcgcc cagggttttg cccgtcacgg tcagcacact gaggtcaaga
      661 tgatctttaa gctggctcat gagcccgcgc agcccgccgg cataataaaa atcttccatt
      721 aaatacttgt cgccgctggg gcgtacattg gccatgaccg ggatcttgcg gctggcgcgt
      781 tcgaagtctt ccaggctgat atcgcaattg gcgcgtttgg cctgcgcaat aatatgaatg
      841 atggcgttgg tcgaacaccc catcgccatg gcaacggtaa tcgcgttctg aaaagcggcc
      901 tgggtctgta ttttgtcggg cgtcacatct tcccatatca tctcgacaat gcgccggccc
      961 gacgcggcgc acatgcggat atggttggag tcggcggccg gaatcgacga tgcgcccgac
     1021 aagctcatgc ccagcgattc cgcaacggca gtcatggtgc tggccgtgcc catggtcatg
     1081 cacgtgccgt aactgcgtgc gatgccgccc tcgatgccca gccattgatt ttgcgttatg
     1141 tttccggcgc gtcgctcatc ccagtatttc cacgagtccg agcccgaacc cagtacgtgg
     1201 ccttcccaat tgccgcgcag cattgggcct gcggggatat aaatggcggg cagcccggcg
     1261 ctggtggcgc ccatgagcag ggcgggcgtt gttttgtcgc agccgcccat cagcacaacg
     1321 ccgtcgatcg ggtggcagcg aagcagctct tcggtgttca tggccagcat gttgcgatac
     1381 aacatggtgg tgggtttgac atacatttcg gccagcgact gggccggcag ttcgatggga
     1441 aatccgccag cctgaaggat gccgcgcttg acgtcttcga cacgcgtttt gaagtgggcg
     1501 tgacagggct ggatgtcgga ccatgtattg atgatcccca ccacgggctt gccgacccag
     1561 tcttcggggg cgtagcccat ttgcatgacg cgtgacctgt gtccaaagcc gcgcaggtcg
     1621 tctggcgcga accagcgagc gctacgtagt gattccggag ttctcatgtg cttcctttac
     1681 ggatgaacga ttaactttgc atgccccagc gacgtactgt tttcttctcg atgagacgaa
     1741 atacgaggtt ttccacgatc agaccgatga tgatgaccgt gagcagcccc gcgaaaacag
     1801 cgggtatgtc aagcagattt ctgttttcga aaatgaacca gcccaggccg cctttgccgg
     1861 atgccacgcc gaaaaccagt tcggcggcga tcaaggtgcg ccacgcgaac gcccagccga
     1921 ttttcaggcc agtcagaatc gaggggaatg cggcaggaat caatattaat gcaacgtagc
     1981 gcaagccccg aagttcataa tttcttccga ccatgcgcag cgtgtgtgaa accgccagga
     2041 accccgcctg cgtgttcagt gccacgaccc agatgacgga gtgcagcaat acgaacacga
     2101 tgctggtggt gcccaggccg aaccagatca gagccagcgg taaaagcgca atggcgggca
     2161 aagggttgaa catggctgtc atgacttcca gaaagtccgt gccaatctgt gtgctgatgg
     2221 ccaggatggt catgattgcg gcaatgacaa tgcccaggga gtagccaatc agtagcaccc
     2281 gaattgacgt ccaggcccga tccagcaaga tcccgtcgcc aatgcggtcg acgaatgact
     2341 ggaacgtatc cgacagcgtc gggaacagca gcggattgcc cagcagtctg ccgtagactt
     2401 cccaggcgat ggccagcagc aggatgatgg tggctttccg cacgaaagca tcgctccaga
     2461 gcttttccag actcgatagc ttgcgcgcca caatcacttt tttttgcgtc tctgaagccg
     2521 ggacggtgtc ctgcttcagg gtaataatag ctcggctcat catggatgcg ccccttcctg
     2581 attaaatagc atgtcgtgaa tttcttgctc gagttcagcg gctaatacgt tgtcgctgga
     2641 tacggcgtcg atattgctga cttcagcttt tacttcgccg ggatgcggcg agagcaataa
     2701 aattcgattg ccgattcgga tcgcttcggc aatcgagtgc gtgacgaata aaacggtgaa
     2761 ctgcgtctcg tgccagagct gcaataactc atcttgcatc tggcgccgcg tcaaggcgtc
     2821 cagcgcggcg aaaggctcgt ccatcaacaa gatgtcgggc tccatcgaca tgccccgcgc
     2881 aatcgccacc cgctgcttca tgccgcccga caaggtgtgc gtgtagctat tcgcgaattg
     2941 ggtcaaatgc actttttcaa cataagcgcg agcgcgttcc tctgcttctt tctttggcag
     3001 ccgttgcgtg accgccagcg gaaacatcac attttcaagc accgttttcc agggcagcaa
     3061 ctgatcgaac tcctggaata ccatcatgcg atccgaaccg ggcctttgga caacggaccc
     3121 tttgattcgg atttcgcctt catccggagt aatgtagccg ccgaccgctt tcagcaaggt
     3181 ggatttgccg cagcccgacg gtccaagcaa cacgaatcga tcgccgctat tgacgtcgaa
     3241 gctgaccttg tcggtggcgg taacgatgct gtcgggtgtt ttatagcgta gggtgacggc
     3301 atccacttca agcaagcagg gcatgattgt ctccggatta gaacgaattg actgtattca
     3361 agaattagct gccattaaga ccggacgatg cgggcaggaa ataatcggtc caattcttgg
     3421 gcatggtttt taggatgccg accttgtgca tgtgctcggc gaacttcatc gagccggcgg
     3481 gcgccatttg aaaagccatc atgccgggct gtttaagcat ttccatcagg tcggcgacgc
     3541 tggttttatc gccgctgatc tcccgataaa tctcgatgga tttttcagtg tttgccttga
     3601 tgtagtcaat ggcttctttc gaagcgtcca atacagcctt gatgattttc ggattgctct
     3661 ttgcaaagtc ggtggtcgta aacagcgttg actgcgacag accgccatcg atgatatcgg
     3721 tcgagcgcaa gacaacgtgc gcgcccggga cggttttcag ttctttgtag tcatacggag
     3781 gcgcggcgaa atgggtgctg atttcgcctt tggggtttgc cagcatggcg gctgcgtccg
     3841 ggtggcccaa ctgtaccgtg ttggcgtcca gttttgacca ttgatcttta ccgaattctt
     3901 tttcgga
//
LOCUS       DATCXU010000019         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_545277,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000019 DATCXU010000000
VERSION     DATCXU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545277"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="purH"
                     /locus_tag="VIR04_00400"
     CDS             complement(<1..401)
                     /gene="purH"
                     /locus_tag="VIR04_00400"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019849.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY9459237.1"
                     /translation="MKIQTALLSVSDKSGIVEFAQALAQRGVHLLSTGGTAKLLAKAG
                     LKVTEVATHTGSPEILDGRVKTLHPKIHGGLLARRDSPEHLKTLEQHHIDRIDLLVVN
                     LYPFRETIAKPNCTLADAVENIDIGGPAMLRS"
     gene            complement(429..668)
                     /locus_tag="VIR04_00405"
     CDS             complement(429..668)
                     /locus_tag="VIR04_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9459238.1"
                     /translation="MSNTNPLEQSVRDRLERYFADLGESEPRDILAMVISCVERPVLQ
                     VALEKTNGNQSKAAEMLGVTRSTLRKKLLAHNLQP"
     gene            complement(705..1724)
                     /gene="dusB"
                     /locus_tag="VIR04_00410"
     CDS             complement(705..1724)
                     /gene="dusB"
                     /locus_tag="VIR04_00410"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY9459239.1"
                     /translation="MHIGQWPLPNSVFIAPMAGGTDRPYRKLCKKLGAGYAVSEMAAS
                     NPLLWDSVKTSRRLDHEGEIDPVAVQIAGADPDMMAEAAVFNIKKGARIIDINMGCPV
                     KKVCNLASGSALLRDENRVARILESVVGACSPLGVPVTLKTRTGWDHESRNAVRIAKL
                     AENIGIAAITLHGRTRCDLYGGQAEYDTIKQVKQALQIPVIANGDIDSPEKAEYVLRY
                     TGADAVMVGRAAQGRPWIFREIAHFLATGLHLQSPCYGELQECLMDHLEDHYKFYGEF
                     TGVRSARKHIGWYLAGLPDTGEALAWINRAQTTHDQTAALQRWFSLHSPNRLITAPIE
                     QALAA"
     gene            complement(1771..2931)
                     /locus_tag="VIR04_00415"
     CDS             complement(1771..2931)
                     /locus_tag="VIR04_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY9459240.1"
                     /translation="MSLPDYDIAITGAGPVGSVLALLLARKASSRQRIALIGKQFIHG
                     DASLPAQSIDPRTLALNHGSRTLLEQLNAWPARSASIETVHVSQRGRLGRALIKSSEL
                     GVSRLGSVVSYNALLAALHQAAAQSGIDLVEAPQASPALARYVQINLGNRVLSCAVAV
                     QSDGARPTGLQRNYHQHAVLTAVRASRPKAGWAFERFTTQGPLALLPHPLGNDLYSVV
                     WCCRPERVTHLQKLDNAAFSAALQDTFGERLGQLECAGARHVFPLALHAGPSLLNPRT
                     VAIGNAAQTLHPVAGQGLNLGLRDAAQLAQALNTWLLQSHGDPGALLGQFAGRRHPDR
                     WLTMGLTDFLPRIFATENHIVQHGCGLSLLALDLSSSIRTPLARHLLQGLRI"
     gene            3047..3514
                     /locus_tag="VIR04_00420"
     CDS             3047..3514
                     /locus_tag="VIR04_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEY9459241.1"
                     /translation="MTRSEIVSSQIMPASRVVRRRNKVLLAWLSFALGLFGAHWWYLG
                     RPRAWMVTAFSLLMLVAAHMYPKWWDNPFFLLLIIPLTDGFIEALIFALKPDDQFDAK
                     YNPLSERKTRTGWSAVIVAMVTVLVGGFVLTWGIAMIVVYIYTAMGWLDGYTF"
     gene            3534..>3867
                     /locus_tag="VIR04_00425"
     CDS             3534..>3867
                     /locus_tag="VIR04_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY9459242.1"
                     /translation="MDLIGWLVPWEFSPTLVLAFLVGGGLFLRGSQVHKVSIARQAFF
                     WFGLILLYLSLHTRLDYYAERMFFIHRIQHLVLHHLGPLLIMGAYPGQVIRAGLPLSW
                     RIRLRNFRL"
BASE COUNT          737 a         1009 c         1168 g          953 t
ORIGIN      
        1 gagcgcaaca tggcagggcc gccgatgtcg atgttttcga cagcatctgc cagtgtgcaa
       61 tttggcttgg cgatcgtttc gcggaacggg tacaaattga ccaccaataa atcgatccgg
      121 tcgatgtggt gttgttcaag cgttttcagg tgctcgggac tgtcgcggcg ggccagcagg
      181 ccgccgtgaa ttttcggatg cagtgttttg acgcgcccgt cgagtatttc gggcgaaccg
      241 gtgtgcgtgg caacctcggt tacttttaat cccgccttgg caagaagttt ggcggtgccg
      301 cccgttgaaa ggagatggac gccgcgttgg gcgagcgctt gggcgaattc gacaatgccg
      361 cttttgtccg agaccgagag taaggctgtt tgaatcttca tgaaggagtg ttgatcagga
      421 gggttgagtt aaggttgcag attatgcgcg agcagctttt tgcgaagcgt gcttcgtgtc
      481 acgccaagca tctcggcggc tttcgattga ttcccattgg ttttttccag tgcgacctgc
      541 aataccggcc gctcgacgca actgatgacc attgcaagga tatcgcgcgg ttcagattcg
      601 ccaaggtcgg caaaatagcg ctcgaggcgg tcgcgcactg attgctccag aggattagtg
      661 ttgctcatag ttagtagttc ttgggtaatt tttatgtgca ttcttcaggc agccagggcc
      721 tgttcaatgg gggcggttat gagccgattc ggcgaatgca aagaaaacca tctttgcaac
      781 gcggcagtct ggtcatgtgt ggtctgggcc cggttaatcc acgcgagcgc ctcgccggtg
      841 tcgggcaggc cggcaagata ccagccaatg tgcttgcgtg ccgatctaac acctgtgaat
      901 tctccgtaaa atttatagtg gtcttcaaga tggtccatca ggcactcttg cagttctccg
      961 taacagggcg attgcagatg caacccggtg gcaagaaagt gggcgatttc gcggaaaatc
     1021 caggggcggc cttgcgcggc gcggcccacc ataaccgcat cggcacccgt ataacgcaat
     1081 acatattcgg ctttttcagg actatcgata tccccgttgg caatgaccgg gatttgcagc
     1141 gcctgcttga cttgtttaat cgtatcgtat tcggcttgcc caccgtataa atcacagcgg
     1201 gtgcgcccgt gcaacgttat agccgcaatg ccgatatttt cggcgagttt ggcgatgcgc
     1261 acggcattgc gagattcgtg atcccagcct gtacgcgtct tcagtgtaac cggtacgccc
     1321 agtggtgaac aggctcccac gacgctttcg agaattcgcg ccacgcggtt ctcgtcgcgc
     1381 aacaatgctg aacccgaggc gagattgcag actttcttga ctgggcatcc catgttgatg
     1441 tcaataatgc gtgcgccttt ttttatgttg aatacggctg cttccgccat catgtcggga
     1501 tccgctcccg caatctggac ggcgacgggg tcgatttcgc cctcatgatc caggcgcctt
     1561 gaggtcttta cgctgtccca aagtagcgga ttgctggccg ccatttcgga tacggcatag
     1621 cccgcaccca gctttttgca tagcttgcgg tagggccggt cggtcccccc cgccatgggg
     1681 gcaatgaata cagagttggg aagaggccat tgaccgatgt gcatgagcgg attttaaccc
     1741 tggattggca tcgcgccatc tgggctgcct ttagatacgt aaaccttgaa gaaggtggcg
     1801 ggccagtggt gttcgaattg atgaacttaa atcgagagcc agcaggctta agccgcagcc
     1861 gtgttggacg atatggtttt cggtggcgaa aatccgtggc aggaagtcgg ttaatcccat
     1921 tgttagccac ctgtcaggat gccggcggcc ggcaaactgg ccaagcaacg cgccggggtc
     1981 gccgtggctt tgaagcagcc aagtgtttaa cgcctgggca agttgcgcgg cgtcgcgcaa
     2041 tccaaggttc agtccctgac cggccacggg atgcagggtt tgcgccgcgt tgccgatagc
     2101 gaccgtgcgc ggattgagca atgatggtcc ggcgtgaagc gccaaaggga aaacgtgacg
     2161 cgcgcctgca cactccagtt gtcccagccg ctcgccgaaa gtgtcttgca gggcggctga
     2221 aaaggcggca ttatcaagtt tttgcagatg cgtgacgcgt tcgggcctgc agcaccacac
     2281 gacgctgtac aggtcgttgc ctagcgggtg cggcaacagg gccagcggcc cttgcgtggt
     2341 gaagcgttcg aaagcccagc ccgccttcgg ccggctcgcg cgcacagccg tcaacacggc
     2401 gtgctgatgg taattgcgct gcaggcctgt gggccgtgct ccgtctgact ggaccgccac
     2461 ggcgcatgac agaacccgat tgcccagatt gatctgcaca tatcgggcca gcgcggggct
     2521 ggcctggggc gcttcgacca ggtcaatgcc gctttgtgcg gcggcctggt gcagcgcggc
     2581 aagcagtgca ttgtaggaaa ctacgctccc aagtcttgat acgccgagct cgctactttt
     2641 gataagcgca cggcccagcc ggccgcgttg cgatacatgg acagtctcga tgcttgcgga
     2701 tcgggccggc cacgcgttaa gttgctccag taaagtgcgg ctaccgtggt taagcgccag
     2761 cgtgcggggg tcgatggatt gagccggaag cgaagcatcg ccatggataa attgtttgcc
     2821 gataagggcg atgcgctgcc ggctgcttgc cttgcgcgcc aacagcaggg ccagcacgct
     2881 gcccacaggc cccgcgccgg tgatggctat atcgtagtcg ggcaggctca tagttttttt
     2941 cttccaagct ttacaatagg cgagacgttg cgaccgatgc gcgcaacatt attgaattcg
     3001 atcgacactg gctgatgcat tcatcgctgg ataatcaagg gtaagtatga cgcggtcaga
     3061 aatagtttca agccagataa tgcccgcttc gcgtgttgtg cgtcgccgga ataaagtgct
     3121 tttggcgtgg ctgtcatttg cgctgggttt gtttggcgcc cattggtggt atttgggccg
     3181 gcctcgagcc tggatggtga cggcattttc cttgcttatg cttgttgcgg cgcatatgta
     3241 tcccaaatgg tgggataacc catttttctt gctgcttata atcccgctca ccgacggttt
     3301 catcgaagcc cttattttcg ctttgaaacc cgatgatcaa ttcgatgcaa aatataaccc
     3361 cctgtcagag cgcaagactc gcacagggtg gagtgccgtt attgtagcga tggttacggt
     3421 gctggtgggc ggtttcgtgc tgacatgggg tatcgcaatg atcgtggtat atatttatac
     3481 cgccatgggt tggctcgacg gttacacttt ttagcgttta gcgggttttc tggatggatt
     3541 tgattggttg gcttgtaccc tgggaatttt ctccaacgct tgtattggcg tttttggtgg
     3601 gcggaggcct gttcctgcgc ggatcgcagg ttcacaaggt ttcgatagcg aggcaggctt
     3661 tcttctggtt tgggctgatt ttgttgtatc tttccctgca tacacgcctg gattattacg
     3721 ccgaacgcat gttcttcatt catcggatcc agcatctggt cctgcatcat ttgggcccgc
     3781 tgcttattat gggggcttac cccggccagg tcatccgcgc cggattaccc ctgtcctggc
     3841 gcattcgtct gcggaatttt cgcctga
//
LOCUS       DATCXU010000020         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_226203,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000020 DATCXU010000000
VERSION     DATCXU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226203"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VIR04_00430"
     CDS             complement(<1..1172)
                     /locus_tag="VIR04_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY9459243.1"
                     /translation="MASSSKKSLIPTTFYDQANNSRRGFYWLTPVVVLILYLCVMGAF
                     IWLQRLHNDSVMFVTIDQETRQQRLIMVVAALSFVIVLSLLALWRYTRFRTRAEAALI
                     AETGFRRAMENSMSTGMRVFDMHGRIAYVNPAFCRMIGWNEADLIGRTTPFPYWLPGR
                     HAQHQETLDLLLSGRTPSSGLEVEAQRRDGSRFTARMYVSPLRDPNGEQIGWMTSMTD
                     ITEPKRIREALTAAHERFMTVLEGLDDAISVVADTSEDLELLFANRTYRRFFGAQSSG
                     HSELLGGRLGRFTDETVEIFSESAQRWFEVHHRILAWTDGRRVRLQVARDITERRTHE
                     EASRVQQEKIQLTSRLTTMGEMASSLAHELNQPLTAITNYNMGAVAMIKSGNAAPE"
     gene            1429..4143
                     /gene="aceE"
                     /locus_tag="VIR04_00435"
     CDS             1429..4143
                     /gene="aceE"
                     /locus_tag="VIR04_00435"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY9459244.1"
                     /translation="MSSLGQVSAMVSAKDDAALETDEWLEALEAVLEREGPERAHYLL
                     ERMIDLARRSGAHVPYSPHTAYLNTIPPGLEPVYPGNLGLEERIRSYVRWNAMAMVVK
                     ANQHHPADGGSLGGHIASFASLATMIACGQNHFWHAESKDHGGDLVFFQGHSSPGMYS
                     RAFLEGRLTQENLDNFRQEVGGKGKGLPSYPHPNLMHDFWQFPTVSMGLGPIMAIYQA
                     RFLKYLHARGIADTSRRKVWVFCGDGEMDEVESLGAIGLASREKLDNLIFVINCNLQR
                     LDGPVRGNGKIIQEFEGVFRGSGWNVIKLIWGSYWDSLLAHDKDGILLRVMEETVDGE
                     YQTYKANDGAYVRKHFFGKDPRLLEMVSRMSDEDIWRLNRGGHDPKKVYAAFAAAAEH
                     KGQPTVVLAKTIKGYGMGHVGQAKNPTHQQKKLDLESVREFRDRFNIPIPDDQLEAIP
                     YFKPAEDSPEMKYMHEHRAALGGYLPQRRVKSDEHFKVPDLQVFKAVLEPTAEGREIS
                     TTQAFVRFLNQILRDKELSPRVVPILADESRTFGMEGLFRQIGIYAPEGQKYTPVDKD
                     QVMYYRETANGQLLQEGINEAGAFASWMAAATSYSVNNRIMIPFFIYYSMFGFQRFGD
                     LAWAAGDMHARGFVLGGTAGRTTLNGEGLQHEDGHSHVLSAQIPNCVSYDPAFAHELA
                     VIMQNGLKRMIENQEDVYYYITVMNENYAQPGLVPGDEDGIIRGMYKFKSTGKNKLRV
                     QLMGSGTILRESIAAQELLEKDWGVGSDIWSMTSFTELRRDGLDCERHNMLHPQAKKP
                     QVPYVTKQLQGTAGPIIVSTDYVKLYGDQIRPYIPEGRTFKVLGTDGFGRSDFRYKLR
                     EHFEINRYFIVLAALHALADEGSVPLEKVAQAIKKYGIDSEKVNPHHA"
     gene            4168..>4427
                     /locus_tag="VIR04_00440"
     CDS             4168..>4427
                     /locus_tag="VIR04_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY9459245.1"
                     /translation="MSNTVQIKVPDIGDFSEVEVIEVLVSEGDSVTAEQSLITVESDK
                     ASMEIPSSGAGVVKSLKVKVGDKIKEGSVILDLESTQADTAPS"
BASE COUNT         1025 a         1168 c         1257 g          977 t
ORIGIN      
        1 atttcagggg cggcgttgcc cgatttgatc attgcaaccg cgcccatgtt gtagttggta
       61 atggccgtga gcggctgatt cagttcgtgt gccagcgatg acgccatttc acccatggtg
      121 gtcaggcggc tggtcaactg aattttttcc tgttgcacgc gcgatgcttc ttcgtgcgtg
      181 cgtctttcgg tgatatcgcg cgccacctgc aagcgcacgc gcctgccatc tgtccaggcc
      241 aggattcgat gatgaacctc aaaccagcgc tgagcggatt ccgaaaaaat ttcaactgtt
      301 tcgtccgtga aacggccaag gcggccgccc aggagttcgc tatggccgct ggattgcgcg
      361 ccgaaaaaac gcctgtaggt gcgattggcg aacagcagtt caagatcttc cgatgtgtcg
      421 gccaccaccg atatcgcgtc gtccagccct tcgagcacag tcatgaatcg ctcgtgcgcg
      481 gcggtcaggg cttcgcggat gcgtttcggc tcggtgatgt cggtcatgga ggtcatccag
      541 ccgatttgct ccccattggg gtcccgtagc ggggacacat acatgcgcgc ggtaaagcgt
      601 gatccatcgc gccgttgcgc ttcgacttcc agcccactgc tgggggtgcg ccccgagagc
      661 aaaagatcga gagtctcctg atgttgcgcg tggcggccag gcagccagta gggaaaaggc
      721 gtggtgcggc cgatcagatc ggcctcgttc cagcctatca tgcggcaaaa cgccggatta
      781 acataggcga tacggccatg catatcgaaa acgcgcatgc ccgtcgacat ggagttttcc
      841 atggcgcggc ggaaaccggt ctcggcaata agcgcggctt cagcgcgtgt ccggaagcgc
      901 gtatagcgcc acaaggcaag caggctcaac acgatgacaa acgacagggc cgcaaccacc
      961 atgatgagac gctgctgccg ggtctcttga tcgatggtca cgaacatgac gctgtcgtta
     1021 tgcaagcgtt gcagccagat aaatgcgccc attacgcaca aatacaggat cagaacaaca
     1081 acgggtgtca accaatagaa gccgcgccgg ctattgttgg cctgatcgta gaaagtggtc
     1141 gggataaggg attttttaga ggagctcgcc atatctctga attaaatgct ttatgcattt
     1201 tagactgtaa cggccatgga gttatttcat attatgaaaa ctcataccgt aaagtgaaat
     1261 ttagcttgat catccggaca gtctttccta gaatccaagt atctactcaa acgagcctga
     1321 tcgttttagg ccacccatgg ccgcgcgcaa cgcgtttcgg gtataaggta attaacggct
     1381 tacacactgt tagtgtttgg tttctgcaat ttatacagga gacatctaat gtcctctctt
     1441 ggccaggtta gcgcaatggt tagcgcgaaa gacgatgcag cccttgaaac agatgagtgg
     1501 cttgaagcgc tggaagctgt attagaacgc gaagggcccg aacgcgctca ctatttgctg
     1561 gaacgcatga ttgatctggc gcggcgctcg ggcgcccatg tgccttattc accccacacc
     1621 gcttacctca ataccatacc ccccggactt gagcccgttt accctggtaa tttgggcctt
     1681 gaagagcgca ttcgctctta tgtgcgttgg aacgccatgg cgatggtagt caaggcaaat
     1741 cagcaccatc ccgccgacgg tggcagcctg ggcggtcaca ttgcctcgtt cgcttcgctg
     1801 gcaaccatga ttgcctgcgg acagaatcat ttctggcacg ctgaaagcaa agaccacggc
     1861 ggcgacctcg tctttttcca gggccattca tcgcccggaa tgtatagccg cgccttcctt
     1921 gaaggccggc tgacgcagga aaacctcgat aatttccgcc aggaggttgg cggcaagggg
     1981 aagggcttgc cttcgtaccc acaccccaac cttatgcacg acttctggca gtttccgacc
     2041 gtgtccatgg gcctgggccc gataatggcc atataccagg cgcgctttct aaaatatctg
     2101 cacgcacgcg gcatcgcgga cacgtccagg cgcaaggtct gggtgttttg cggcgatggc
     2161 gaaatggatg aagtggaatc gctgggcgct atcggcctgg catcgcgtga aaaactcgac
     2221 aatcttattt tcgtcatcaa ttgcaatctg cagcgccttg acggtcccgt gcgtggcaat
     2281 ggcaaaatca ttcaggaatt cgaaggcgtt ttccgcggca gcggctggaa tgtgatcaag
     2341 ctgatctggg gcagctattg ggattcgctg cttgcacatg ataaagacgg cattttgttg
     2401 cgcgtcatgg aagaaactgt tgatggcgaa tatcagacct acaaggccaa tgatggcgcc
     2461 tatgtgcgca agcatttctt cggcaaagat ccgcgcttgc ttgaaatggt cagccgcatg
     2521 agcgatgagg acatctggcg tctgaatcgc ggcggtcacg atccaaaaaa agtgtacgcc
     2581 gcattcgccg ccgccgccga acacaaaggt cagccgaccg ttgtcctggc gaagacgatc
     2641 aagggctatg gcatgggcca tgtcggccag gcgaaaaacc cgacgcacca gcagaaaaaa
     2701 ctggacctgg agtcagtgcg cgaattccgg gatcgcttca atatccccat ccctgacgac
     2761 cagctcgaag ccatacccta cttcaagcca gccgaagatt cgcctgaaat gaagtatatg
     2821 catgaacatc gcgcggcgct gggcgggtat ttgcctcagc ggcgcgtcaa gtccgatgaa
     2881 cacttcaagg tgcccgatct gcaagtgttc aaggcggtgc tcgaacccac tgcggaaggc
     2941 cgcgaaattt ccacaacaca agcctttgtg cgttttctga atcagatttt gcgcgacaag
     3001 gaacttagcc cgcgggtagt gccgattttg gcggacgaat cacgcacgtt cggcatggaa
     3061 ggcttgttcc gtcaaattgg tatttacgcg cccgaagggc agaagtacac cccagtcgat
     3121 aaagaccagg tgatgtacta ccgcgaaacg gcgaatggcc agttgcttca ggaaggcatc
     3181 aatgaagcgg gcgcgtttgc ttcatggatg gcggccgcaa cgtcctactc ggtaaataat
     3241 cggatcatga ttccgttttt catttactac tcgatgttcg gctttcagcg ttttggcgat
     3301 ctggcttggg cggcaggcga tatgcatgcg cgcggctttg tgctcggcgg cacggcgggg
     3361 cgcaccacgc ttaacggaga aggcctgcaa cacgaagatg gacacagcca tgttctgtcc
     3421 gcgcaaattc caaactgcgt ttcctacgat ccggcgtttg ctcacgagct tgccgtgatc
     3481 atgcaaaacg gtttgaagcg catgatcgaa aatcaggaag acgtttacta ctacattacc
     3541 gtcatgaatg aaaactacgc ccaacccggc ctggtcccgg gtgacgagga tggcatcatt
     3601 cgcggcatgt ataaatttaa gtcaaccggg aaaaacaagc tgcgggtgca attgatgggt
     3661 tccggcacga tcttgcgcga atccatcgcg gcccaggagt tgcttgaaaa agactggggc
     3721 gtgggttccg atatctggag catgaccagc ttcaccgaac tgcggcgcga cgggcttgat
     3781 tgcgagcgcc acaatatgct gcatccgcaa gcgaaaaagc cgcaggttcc ttatgtcacg
     3841 aagcaattgc aaggcactgc cgggccgatc atcgtgtcga ccgattacgt caagttgtat
     3901 ggggatcaga ttcggccata tataccagag ggccgtactt tcaaagtgct gggcaccgat
     3961 ggcttcgggc gctccgattt ccgctataaa ttgcgcgaac acttcgaaat caaccgctat
     4021 ttcattgtct tggccgcatt gcatgcgctg gcagacgagg ggtctgtgcc gcttgaaaag
     4081 gtcgctcagg caattaaaaa atacggtatt gattccgaaa aagtcaatcc gcatcacgcg
     4141 taaaaaggaa aaggggagga ggctcatatg agtaatactg tccagattaa ggttccggac
     4201 atcggcgact ttagcgaggt ggaagttatc gaggtgctgg tttccgaggg cgatagcgtg
     4261 acggccgagc aaagtcttat cacggtcgag tcggataagg cttctatgga aattccgtct
     4321 tccggcgcgg gagttgtgaa gtcgttgaaa gtcaaagtcg gcgacaagat caaggaaggt
     4381 tcggtcattc tcgatcttga gtcgacgcaa gcggataccg ccccgtc
//
LOCUS       DATCXU010000021         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1011035,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000021 DATCXU010000000
VERSION     DATCXU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011035"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VIR04_00445"
     CDS             complement(<1..1437)
                     /locus_tag="VIR04_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY9459246.1"
                     /translation="MYSGADSLCDTLLTNDIDVCFANPGTSEMYFVAALDKKPRMRCV
                     LGLFEGVVTGAADGYARMADKAAATLLHLGPGLANGLANIHNAHRAHTPMVNIVGDHA
                     TGHLKYDAPLTTDIESLARPMSDWVRRIRSAADVSTCAAAAIEAAHTGQGQIATLILS
                     ADASWSESPAKIQRAAPAGFTQVEAGALRQASEALRNGRKTVILLSGKALRSKALETA
                     SRIAVKTGARLLAQQSNGRVERGAGRVMIDRMPYNVDLALKALAGTEQLILIGAKTPV
                     AFFAYPGKPGALTPPDCKIIPLTRPTDDLAGALGALADSVGVTNDVAAITAKRSAGEL
                     PTGPLTAAAMIRAIGALSPENAIICDESISSGRNSFAATFGAAPHDFLQNTGGAIGAG
                     IPLATGAAVACPDRKVILMQADGSGMYTLQALWTQARDQLDVVTIIFANRSYAILHNE
                     LKMVGAGEPGRNARRMLDLDSPAFDWVHM"
     gene            complement(1520..>2071)
                     /locus_tag="VIR04_00450"
     CDS             complement(1520..>2071)
                     /locus_tag="VIR04_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY9459247.1"
                     /translation="AAKAAKAGGKELSFASPGVGSSVHLTGAYFEQVAGIHMLHVPFK
                     GASQSIPAVAGGQVDVLIDNLPSSLAQIKEGSRLRGIAVTSAKRSPALPNIPTVAESG
                     LPGFDVIAWFALYAPAGTPKPIVDKLIAAAKTALESPDVKAKFASFGAQAGTLFGSDL
                     GAFQHKEVERWGKVIKDRHITTQ"
BASE COUNT          378 a          624 c          649 g          420 t
ORIGIN      
        1 catatgcacc cagtcgaacg cgggactatc caaatccagc atgcgccgtg cgttgcgccc
       61 cggttcaccc gcccccacca tcttcagctc gttgtgcaaa attgcgtagc tgcggttggc
      121 aaaaataatg gtgacgacgt cgagctggtc gcgagcctgc gtccacaacg cctgaagcgt
      181 gtacatgccg ctgccgtctg cctgcatcag gatgaccttg cgatcggggc acgcaacggc
      241 cgctccagtt gccaggggga tgcccgcacc aatggcgcca cccgtgtttt gcagaaaatc
      301 gtgcggcgcg gcgccaaagg tggcggcaaa agagtttctg cctgagctta tcgattcatc
      361 acaaatgata gcgttttcgg gacttaaagc gccgattgcc cggatcatcg cggcggcggt
      421 tagcggcccc gtgggcaatt cgcccgcgct gcgcttggcc gtaattgccg ccacgtcgtt
      481 ggtcacgcca accgaatcgg ccagtgcacc gagcgcgccg gcaagatcat cggtggggcg
      541 tgtaagagga ataatcttgc agtcgggcgg cgttagcgcc ccaggtttgc cagggtaggc
      601 aaaaaacgcc actggcgttt tggcgccaat caaaataagc tgctctgtgc cggccagcgc
      661 cttcagcgcc agatcgacgt tatagggcat gcgatcaatc atcacgcgcc cggcgcctcg
      721 ctcaacgcgg ccgttggatt gctgcgccag caatcgcgcg cccgtcttga cggcaatccg
      781 gctggcagtt tccagcgctt tgctgcgcag cgccttgccc gatagcagga tgacagtctt
      841 gcgtccattg cgtagcgctt cgcttgcctg gcgcagcgcg ccggcttcga cctgagtaaa
      901 gcctgccggc gcggcgcgct gaattttggc tggcgactcg ctccaggacg catcggcgga
      961 gagaatcagt gtggcaattt gaccttgccc ggtatgcgct gcttctatgg ctgccgccgc
     1021 gcaagtcgac acgtcggccg cggaacggat ccggcgcacc cagtccgaca tcggccgcgc
     1081 caggctttcg atgtcggtgg tcagtggagc gtcgtatttc agatggccgg tggcgtggtc
     1141 gccgacaata ttgaccatcg gcgtatgggc acggtgcgcg ttatgaatat tggccaggcc
     1201 gttggccagg ccgggcccca ggtgcagaag cgtggcggcc gccttgtcgg ccatgcgcgc
     1261 gtagccatcg gccgcgcccg ttaccacgcc ttcgaacaag cccagaacac agcgcatgcg
     1321 cggctttttg tcgagcgcag cgacgaagta catttccgac gtgcccgggt tagcgaaaca
     1381 cacgtcgata tcattggtca gcaacgtatc gcaaagactg tctgcgccgc tatacatgcg
     1441 agagtgctcc ttattttctg tttgttgttt cggtcgatcg gacaagcctg ctcgggcagg
     1501 cttgttgttg actacgcgat cactgggttg tgatgtggcg gtctttgatc accttgcccc
     1561 agcgttcgac ctctttgtgc tgaaaggcgc ccaggtcgct tccaaacagc gtccctgctt
     1621 gtgcgccgaa tgacgcaaac ttggctttga cgtcgggcga ttccagggca gttttggcgg
     1681 cggcaatcag cttgtccaca atgggtttcg gcgtgccggc gggtgcataa agggcgaacc
     1741 acgcaattac gtcaaagccg ggcaggccgg attccgccac agtgggaatg ttggggaggg
     1801 ccggagaacg cttcgccgag gttacggcga tgccccgcaa cctggagcct tccttgatct
     1861 gagccaggga actgggcagg ttgtcgatca ggacgtcgac ctgaccgccg gcaacggcag
     1921 gtatggattg cgacgcgccc ttgaaaggca catgcagcat atggattccc gcaacttgct
     1981 cgaagtaggc gccagtcagg tgcaccgatg acccaacccc gggcgatgcg aaggacaatt
     2041 ctttgccgcc ggctttggcg gcttttgccg c
//
LOCUS       DATCXU010000022         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_225798,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000022 DATCXU010000000
VERSION     DATCXU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225798"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIR04_00455"
     CDS             <1..198
                     /locus_tag="VIR04_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459248.1"
                     /translation="MSACSDCPYLSRLSPKRAVCCCDEVKAHHLADREALTRELAECK
                     EQQTIEPMNRAARRRAAKLAR"
     gene            211..726
                     /locus_tag="VIR04_00460"
     CDS             211..726
                     /locus_tag="VIR04_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEY9459249.1"
                     /translation="MTIECHGLDTPELVCFYEQDFYVLSNFSAFRIQWRGRDFDTSEA
                     AYHWERFATGHDNGRSPAHGALAASIADKIRFAPSAHEAFKLAQANKQHQRPDWDDAK
                     VGVMLNILRAKADQHEYVKRKLLATGNRHLVENSWRDDFWGWGPNKDGQNMLGKLWMT
                     IRRDLRQQQAA"
     gene            743..952
                     /locus_tag="VIR04_00465"
     CDS             743..952
                     /locus_tag="VIR04_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459250.1"
                     /translation="MIYAQIKSGHKLHLALEGGEVDREGVRIERGMLSHPLCGQRMTE
                     PNYRMTINVPLANACRKCRAIAAKQ"
     gene            964..1197
                     /locus_tag="VIR04_00470"
     CDS             964..1197
                     /locus_tag="VIR04_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459251.1"
                     /translation="MSDLFEPDTFQEIRIGNIGVMAAGGTVDISRPEDVRENKTVAKP
                     EWERRTYMTLSPALARLLADALLKAANACGGGW"
     gene            1281..1478
                     /locus_tag="VIR04_00475"
     CDS             1281..1478
                     /locus_tag="VIR04_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459252.1"
                     /translation="MPLEDHYDFTMRMTASDFGCLKASVDSLLRRAIASDHTEYQHQL
                     EQLQQKIEQSWEGWRSKHLSR"
     gene            1493..1792
                     /locus_tag="VIR04_00480"
     CDS             1493..1792
                     /locus_tag="VIR04_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459253.1"
                     /translation="MAQPASAMMKETADLCRKAVVPAEAPVLRKLLDLAACDLEAASS
                     IFRHNIEIPNGQIDRLKAALKPLAALAVPEKPTGNAGAYSIRFTDIQAAKAALSQ"
     gene            1803..2024
                     /locus_tag="VIR04_00485"
     CDS             1803..2024
                     /locus_tag="VIR04_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459254.1"
                     /translation="MRDILHVASPPPAAQRNRALTTTPHHIGVMVANEYIGYGQIIRE
                     AHHGYVLHCLKKQIFRAVTDCIAQHSKPA"
     gene            2035..2298
                     /locus_tag="VIR04_00490"
     CDS             2035..2298
                     /locus_tag="VIR04_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459255.1"
                     /translation="MRINVYSQELTREAHLIQKESNTGLIYSAVQLMLHSSDRLHHPP
                     ADDDRSAVTFWLPKSKERRRELAETFRHMANLVAAAPLETGLD"
     gene            2310..2567
                     /locus_tag="VIR04_00495"
     CDS             2310..2567
                     /locus_tag="VIR04_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459256.1"
                     /translation="MAKITDKQRACLEKMATGAALVCSNSPGTDKIDCFVEGRPSLSV
                     HGATVNGLMARGLIRGGGSYGQFSTYCEITDAGRAAIAPAT"
BASE COUNT          598 a          742 c          755 g          475 t
ORIGIN      
        1 atgtcagcct gttcggactg tccatacctc agcagactgt cgccgaaacg cgcggtgtgc
       61 tgctgcgacg aagtgaaagc acatcacctg gcagacaggg aagcgctgac gcgcgaactg
      121 gccgaatgca aggaacagca aaccatcgaa ccgatgaacc gtgcagcccg gcgtcgtgcc
      181 gcaaagctgg cccggtaaag gagtcacaac atgaccattg aatgccacgg tctcgacaca
      241 ccggaactgg tgtgtttcta cgaacaggat ttttacgtcc tgtcgaactt ctctgccttc
      301 cgcatccagt ggcgcgggcg cgacttcgac accagcgaag cagcctatca ctgggaacgg
      361 ttcgccaccg gccatgacaa cgggcgctcc ccggcgcatg gtgcgctggc ggcgagcatt
      421 gcggataaaa tccgcttcgc accttcggcc catgaagcgt tcaagctagc ccaggcgaac
      481 aagcagcacc aacgcccgga ttgggatgat gcgaaggtcg gcgtcatgct caacatcctg
      541 cgcgctaagg ctgaccagca cgagtacgtc aagcgcaagc tgctggcgac cggcaaccgt
      601 cacttggtcg aaaatagctg gcgcgatgac ttctggggct ggggtccgaa caaggacggc
      661 cagaacatgt tgggcaaatt gtggatgacg atccgccgcg atctccgtca acagcaagcc
      721 gcctaaccgg ggagcgccag ccatgatcta cgcgcagata aagtcaggcc acaagctgca
      781 tctggcgctg gaaggcggtg aggtggaccg cgaaggtgtc cgtatcgagc gcggcatgtt
      841 gagtcaccca ctgtgtgggc agcggatgac tgaacccaat tatcgcatga ccatcaatgt
      901 gccgctggcg aatgcctgtc ggaagtgccg cgccatagcg gccaagcagt aacggagcgg
      961 aaaatgtcag acctatttga acccgatacc ttccaggaaa tccgcatcgg caatattggt
     1021 gtgatggctg ctggcggaac ggtggacatt tcacggccag aagatgttcg agagaacaaa
     1081 acagtcgcaa agccggaatg ggagcgccgc acctatatga ctttgagccc ggcgcttgcc
     1141 cgcttgttgg ctgatgcact gttgaaggca gcgaacgctt gcggtggggg ctggtaatgg
     1201 ggtgcagagc gtgtgggtgg atcatcgttt tggctggcat atttgccgcc atttttcttt
     1261 ctcgctgagg agggaatatc gtgccgctcg aagaccatta cgatttcacc atgcgcatga
     1321 cggccagcga cttcggctgc ctcaaggcat cggtggactc gctgctgcgg cgcgcaatcg
     1381 cctccgacca taccgaatac caacatcagc ttgagcaact tcaacagaag atcgaacagt
     1441 cgtgggaagg ctggcggtcg aaacatctat cccgataacg gagcgtttga acatggcaca
     1501 gccagcaagt gcgatgatga aagaaacggc agacctttgc cgcaaggctg ttgtgccagc
     1561 cgaagcgcca gtattgcgca agctgcttga cttggccgca tgtgatcttg aggcggcatc
     1621 cagcatcttt cgtcacaaca tcgaaattcc gaacggtcag attgatcggc tcaaggccgc
     1681 gcttaaaccg ctcgccgcgc tggcagtccc agagaaaccg acaggcaacg ccggggccta
     1741 ttcaatccgg ttcacagaca ttcaagccgc caaagcggcc ctgtcgcaat aggcgagcga
     1801 ctatgcgcga tattcttcac gttgcctcgc cgccaccagc cgcacagcgc aatcgggcgc
     1861 tgaccacgac gccgcaccac atcggcgtga tggtcgctaa cgaatatatc ggctacggcc
     1921 agatcatccg cgaggcgcac catggctatg tgctgcattg cctcaaaaag cagattttcc
     1981 gcgcggtgac ggattgcatc gcgcaacatt caaagcctgc gtaaaggagc caccatgcgt
     2041 atcaacgtct acagccaaga actgacgcgg gaagcgcacc tgatccagaa ggaaagcaac
     2101 accggcctga tatacagcgc cgtgcagttg atgcttcatt cttccgaccg cctgcaccat
     2161 ccgcctgccg atgatgaccg tagcgccgtg acgttctggc ttccaaaatc gaaagagcgt
     2221 cgccgcgaat tggctgagac tttccggcac atggcaaacc ttgtggctgc tgcgccgctc
     2281 gaaaccggcc tggattgaag gagcccatcg tggcgaaaat aaccgacaag caaagggcat
     2341 gtcttgaaaa gatggcgaca ggagcggcgc tggtatgcag caactcgccg ggcacggaca
     2401 agattgattg ctttgtcgaa ggtcggcctt ctctgtccgt gcacggcgcg accgtcaatg
     2461 gcttgatggc gcgtggcctt attcgaggcg gcggaagcta cgggcaattc agcacctatt
     2521 gtgaaatcac cgatgctggc cgcgccgcca ttgcaccggc aacctgaaag
//
LOCUS       DATCXU010000023         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1169787,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000023 DATCXU010000000
VERSION     DATCXU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169787"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="murE"
                     /locus_tag="VIR04_00500"
     CDS             complement(<1..1113)
                     /gene="murE"
                     /locus_tag="VIR04_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEY9459257.1"
                     /translation="MTAQFIISWLREHVKRGAHLCLDSRQVNPGDVFFACPGLVTDGR
                     LYVDVAINRGAGAIVAQADFERSGALPAHVPVLTVDGLSGLLGRVASEWYGEPSRAMS
                     VVAITGTNGKTSTVQWLAAALKAEGVACGVIGTLGISLPDGSSLGSALTTPDVVTMHR
                     SLAAMRDAGAAVVAIEASSIGLDQGRLDQVHVEIAGFTNLTHDHLDYHHTLENYKAAK
                     FALFALPGLRAAVINVDDPAGIELLESIPAVESVTYSLGIAGDARIQARDIHAGAYGL
                     VFNLTMPGGAAQIVTRLVARHNVSNLLLVAGVLHKLGWSLSRIARTLAILRPVPGRLQ
                     IIDAPACGDASIALPMVVVDYAHTPDALQRALAALRD"
     gene            complement(1110..2846)
                     /locus_tag="VIR04_00505"
     CDS             complement(1110..2846)
                     /locus_tag="VIR04_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY9459258.1"
                     /translation="MKRVRFYDSPVLNVQMPVWRARLVLILLMLGFVALAAKALYLQG
                     ISTEFLQQQGELRYERTLVLPATRGKIFDRSGSAVLASSIPARAIWAIPEDTKAATPD
                     ELNQLAKLLDLPVKDIQSRLTDDDKNFVYIKRQVSLDTAAAIRKLKIPGFHQLDEMRR
                     YYPDGNVTAHVLGFTNIEDKGIEGVELAFNSELSGRPGSRHVIKDRLGRVVEDIQAIV
                     PPVDGQDLSLSIDAGIQFDTYAALKVAMEEHKAKAAAAIVVNAQTGEILALVNLPAYD
                     PNDRDDRKGPALRNMAMADMFEPGSIMKPFTAALALDLHRITPSTMFNTGNGRYQYQG
                     ATIHDVSSNGRLNVAGILRRSSNIGMTMISELLTSKEMWNKFTELGFGRAPQTNFPGL
                     ATGRLRPWERWRPIERATMAYGYGLSVSLLQIAHAYTAFARNGDMVSLTLLKRQGKPT
                     SVQVYSPQVAGLIRAMLEAAAGPDGAKLAQVQGYRVAGKSGTVRKIIDGHYSKSQYRG
                     SFVGFAPVSNPRIVVAVTIDDPTTGGYYGGKIAAPVFSSIVASSLRRMGVQPDAPIQS
                     LVAVGPREGASR"
     gene            complement(2843..3157)
                     /gene="ftsL"
                     /locus_tag="VIR04_00510"
     CDS             complement(2843..3157)
                     /gene="ftsL"
                     /locus_tag="VIR04_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY9459259.1"
                     /translation="MGRLSLLFAALLMILSMSLVTARFQSRQLFVVSDRLDATARQLG
                     TDWLRLQLERAELARNAHIDKVARDDLKMVSTSPQRTIYIKGGGSEAIAPATQAKAGR
                     RP"
     gene            complement(3157..>3412)
                     /locus_tag="VIR04_00515"
     CDS             complement(3157..>3412)
                     /locus_tag="VIR04_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY9459260.1"
                     /translation="AAARPAAVHARMPIYERDLPQPILDSLGRVRASAEEIAANPRAR
                     SAVLRVAQRTATPLPKDQGASFVQPLHITGKPAGHSRRRK"
BASE COUNT          739 a         1058 c          958 g          657 t
ORIGIN      
        1 gtcgcgcagg gccgccaagg cccgctgcaa agcatcgggc gtatgggcat agtcgaccac
       61 gaccatcggc agcgcaatcg aggcgtctcc gcatgcgggt gcatcaatga tttgcagacg
      121 cccgggaacc ggcctcaaaa tagccaaggt tcgggcaatg cgggacaaag accaccccaa
      181 tttgtgcagc acgccggcaa ccagcagtaa attcgacaca ttgtggcgcg ccaccaggcg
      241 ggtcactatc tgggcggcgc cgccaggcat tgtcagatta aaaaccaaac cgtatgcgcc
      301 ggcatggata tcgcgcgcct gaatgcgcgc gtcgccggca atgcccaacg agtaggtaac
      361 cgactccaca gccggaatcg attcgaggag ctcaataccg gcggggtcgt cgacattgat
      421 tactgccgcg cgcaagccag gcaatgcaaa cagggcgaac ttggccgcct tgtagttttc
      481 cagcgtgtgg tgataatcga gatggtcatg cgtcagattg gtgaacccgg ctatttccac
      541 gtgcacctga tccagacgcc cctggtctag tccgatggac gatgcctcga tcgccaccac
      601 agccgcgccc gcgtcgcgca tggccgccag gctgcgatgc atcgtgacga catcgggggt
      661 cgtcaacgcg ctacccagac tcgatccatc gggcagactt atacccaagg ttccaatcac
      721 gccgcaggcg acaccttccg cctttaaagc cgccgccagc cattgcacgg tactggtctt
      781 gccgttcgta cctgtgatcg cgaccaccga catggcgcgc gagggttcgc cataccattc
      841 gctcgccacc cgccccaaca ggccggacag cccgtcgacc gtcagaacag gcacgtgcgc
      901 gggcaacgcg ccggagcgtt cgaaatcagc ctgtgcaacg atggcgcccg ccccgcggtt
      961 aatagccaca tcgacatata aacggccgtc agtgaccagg cccggacacg caaaaaacac
     1021 atcgcccgga ttaacctggc gcgaatccag acatagatgc gcgccacgct ttacgtgttc
     1081 ccgcagccat gaaatgatga attgagctgt catcgcgagg ccccttcccg aggtcccaca
     1141 gccaccagcg attgtatcgg cgcgtcgggc tgaacgccca tgcgtctcaa gctgctggcc
     1201 actatgctgg aaaaaaccgg cgcggcgatt ttgccgccgt aataaccgcc ggtagtcgga
     1261 tcgtcgatcg tgaccgcaac tacgatgcgc ggattggaca ccggcgcaaa gccgacgaac
     1321 gagccgcgat actgcgactt gctgtagtgc ccgtcgataa ttttgcgcac cgtgccgctt
     1381 ttgcctgcta cgcgatatcc ttgtacttgc gccaacttgg cgccgtccgg acccgccgcg
     1441 gcttccagca tggcccgaat caaacccgcc acctgcggcg aatacacctg aacgctggtc
     1501 ggcttgccct ggcgcttgag caaagtgagc gaaaccatat cgccattgcg cgcaaacgcg
     1561 gtgtaggcgt gagcgatttg cagcaacgac accgaaaggc cgtagccgta tgccatcgtt
     1621 gcccgttcaa tcggacgcca gcgctcccag ggacgcaagc gcccagtggc caatccaggg
     1681 aagttagttt gcggcgcacg gccaaagccc agctcggtga atttgttcca catttccttc
     1741 gacgtcagca gttcggaaat catggtcatg ccgatattgc tggaacggcg caaaatgcct
     1801 gccacattaa gccggccatt actgctcaca tcgtgaatcg tcgcgccctg atattggtag
     1861 cggccgtttc cggtattaaa catggtggag ggcgtaattc tgtgcaaatc cagcgcaagc
     1921 gccgccgtaa acggcttcat gatcgacccg ggttcaaaca tgtctgccat cgccatgttg
     1981 cgcaaggccg ggcccttgcg gtcgtcgcgg tcgttcggat cgtaggccgg aaggttcacc
     2041 agagccagaa tttcgccggt ttgagcattc actacgatcg ccgccgccgc cttcgccttg
     2101 tgttcttcca tggccacctt caacgcggcg taggtatcga actgtattcc ggcatcgatc
     2161 gacaggctta aatcctgacc atcgaccggc ggcacaatgg cctgtatgtc ttcaacaacc
     2221 cttcccaagc ggtctttgat gacgtggcgg cttccggggc ggccggaaag ctcggaattg
     2281 aaagccaatt caacaccttc aattcccttg tcttcgatgt tggtgaatcc caggacgtgc
     2341 gcggtcacat tgccatcggg ataataacgg cgcatttcat cgagctgatg aaagcccggg
     2401 attttaagct ttcttatcgc ggcggcggtg tcgagggaca cctgacgctt gatataaacg
     2461 aaatttttgt cgtcatcggt caagcggctc tgaatgtctt ttactggcaa atccagcaac
     2521 ttggccaatt ggttcaactc atccggcgtt gccgctttgg tgtcttcggg aatggcccag
     2581 atagcgcgtg cgggtatgct cgaagccaga accgccgaac cgctgcgatc aaatattttg
     2641 ccacgcgtag ccggcaacac cagcgtgcgc tcataacgca attcaccttg ttgctgcaaa
     2701 aattcagttg atatgccttg caaatacagc gccttggcgg ccagagcgac gaagccaagc
     2761 atcaaaagaa tcagcaccag cctggcgcgc catacgggca tctgaacatt cagaacaggg
     2821 ctatcgtaga accggacccg tttcatggac gcctccccgc cttggcctgc gttgccggcg
     2881 cgatcgcttc ggaaccacca cccttgatgt atatagtgcg ttgcggcgag gtggacacca
     2941 ttttcagatc atcgcgagcg accttgtcga tatgcgcgtt gcgcgccaat tcggcgcgct
     3001 ccaactgcaa acgaagccaa tccgtgccaa gttgacgcgc cgtggcatca agcctgtccg
     3061 agacgacaaa caattggcgc gattgaaaac gcgccgtgac cagggacatc gacagaatca
     3121 ttaacaacgc tgcgaagagc aagctgaggc ggcccattat tttcgcctcc tggaatgccc
     3181 agccggcttg ccggtaatat gtaacggctg aacgaacgac gcgccctgat ccttgggcaa
     3241 tggcgttgcc gtgcgttgcg ccacacgtaa aacggccgag cgagcacgcg ggttggcagc
     3301 gatttcctcg gccgaggccc gcacacgccc gagcgaatca agaatgggct gaggcagatc
     3361 tcgctcgtaa atcggcatgc gcgcgtgcac ggccgccggc cttgccgcag cg
//
LOCUS       DATCXU010000024         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_224949,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000024 DATCXU010000000
VERSION     DATCXU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224949"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1083)
                     /gene="argC"
                     /locus_tag="VIR04_00520"
     CDS             complement(19..1083)
                     /gene="argC"
                     /locus_tag="VIR04_00520"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY9459261.1"
                     /translation="MTSAASSRIKVGIVGGTGYTGVELLRLLSQHPNVQLAAITSRQE
                     DGTPVADMFPNLRGWVDLRFQAPEKAALDQCDVVFFATPHGVAMSQAEALLEKGVRII
                     DLAADFRLQDTRVFEQWYKMPHACPNILTEAVYGLVELNRAKIAHARVIGNPGCYPTT
                     VILGLAPLIQGERQLVDTTHIIADCKSGVSGAGRKAAVGTLFSEASDNFKAYGVAGHR
                     HHPEISEQLEILAGAPVGVTFVPHLIPMIRGMFATLYARILPDALDVDFQELFEQRYA
                     DEPFIDVMPAGSLPETRSVRASNYVRIAVHRPENEDQLVVLVAQDNLVKGASGQAVQN
                     MNLMFGLPEAAGLEQVAVLP"
     gene            complement(1287..1679)
                     /gene="rpsI"
                     /locus_tag="VIR04_00525"
     CDS             complement(1287..1679)
                     /gene="rpsI"
                     /locus_tag="VIR04_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY9459262.1"
                     /translation="MIGNWNYGTGRRKTSVARVFLKKGTGKIVVNGKPVDEYFARETG
                     RMVVRQPLTLTNHLETFDFHVNVRGGGESGQAGAIRHGITRALIDYDETLKPPLKQAG
                     LVTRDAREVERKKVGFHKARRRKQFSKR"
     gene            complement(1689..2117)
                     /gene="rplM"
                     /locus_tag="VIR04_00530"
     CDS             complement(1689..2117)
                     /gene="rplM"
                     /locus_tag="VIR04_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY9459263.1"
                     /translation="MKTFVAKPHEVKRDWYVIDARGKVLGRVASEVARRLRGKHKPEF
                     TPHVDTGDYIVIINAADIVVTGNKAQAKKYYRHSGYPGSITETTFEKMQQRFPGRAIE
                     KAVKGMLPKGPLGYAMAKKLKVYAGAEHPHSAQQPKPLEF"
     gene            complement(2284..>3079)
                     /locus_tag="VIR04_00535"
     CDS             complement(2284..>3079)
                     /locus_tag="VIR04_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY9459264.1"
                     /translation="APIAGRGASMQRDYWYSAQRRPELLASAQAVGRYAAQRTLSRLS
                     ARRIKTGHFPVLFEAPLAVGLLGALVQATSGGALYRKASFLLDSLGQPVLSDHLDVSE
                     KPHIIAGMGSSPFDNEGVRTQSREVVTGGVLQGYFLSTYTARKLKMRTTGNAGGSHNL
                     DLTSRLTQPADDLRAMLKKMGMGLLVTELIGQGVNYVTGDYSRGAFGYWVENGEICHA
                     VQEVTIAGNLADMFKRIVAVGSDVIVRGSKCTGSILLEQMAIAGSR"
BASE COUNT          680 a          915 c          828 g          656 t
ORIGIN      
        1 ccccttgcgg cgcccatttc agggcaatac cgcgacctgc tccaagccag ccgcttcagg
       61 caaaccaaac atcaaattca tattctgaac ggcctgtccg gaagcgccct tgacaagatt
      121 gtcctgagca accagcacca ccagttgatc ttcgttttca ggacgatgaa ccgcaatcct
      181 gacataattc gaggcacgca ccgagcgcgt ttcaggcagg ctgcccgctg gcataacgtc
      241 gatgaaaggc tcgtcggcgt aacgctgttc gaatagttcc tgaaagtcga catcaagcgc
      301 atcgggcaat atacgcgcat aaagggttgc gaacatgccc cgaatcatgg gtataaggtg
      361 cggcacgaac gttaccccca ccggggctcc ggccagaatt tcaagctgct cggagatctc
      421 ggggtggtga cgatggcccg ccacaccata tgccttgaaa ttgtcgcttg cttcggaaaa
      481 taacgtgccg acagccgcct tgcgcccggc gcccgataca cccgatttgc aatcggcaat
      541 aatgtgcgtg gtgtcgacca gttgacgttc gccttgaatc agcggcgcca gtccaagaat
      601 aacggtcgtg gggtaacacc ccggattgcc aataacccgg gcatgcgcaa tttttgcccg
      661 attcagctca accaggccgt acaccgcttc tgtcagaata ttggggcagg catgcggcat
      721 tttgtaccat tgctcgaata cgcgcgtgtc ttgcaaacgg aaatcggcgg caagatcgat
      781 gatacgcacc cctttttcaa gcaaggcttc ggcctggctc atggcgaccc catggggcgt
      841 ggcaaaaaac acgacgtcgc attggtccag cgccgctttc tcgggggcct gaaaacgcaa
      901 atcgacccat ccgcgcaaat tggggaacat gtcagccact ggggttccat cttcctgacg
      961 cgaggtgatt gccgcaagtt gcacattggg atgctgggac agaagacgca gtagttcgac
     1021 tccggtatag cccgtgccgc ccacaatacc aaccttgatc ctggaagatg ctgccgaagt
     1081 catgataatt cccatagaac gaacatatgc gccgcgtata agctacatta tgcaataacc
     1141 acactgcggc gcaaaccggc gccgcgacaa aaccgactat tgatgcccac tgcggcgcga
     1201 tgacagccac gccatggcaa aaaccccgga acaagccggg gtttttgatc gcaccaggct
     1261 gtttccggcc cggggtccag caccaattag cgcttgctga actgcttgcg acggcgggcc
     1321 ttgtggaagc cgaccttttt acgttcgacc tcgcgggcat cgcgggtcac caaacccgcc
     1381 tgcttgagtg gcggtttaag cgtttcgtcg tagtcgatga gcgcgcgcgt aatgccatgc
     1441 cggatcgccc cggcttgacc gctttcgcca ccaccgcgca cattgacgtg gaaatcgaac
     1501 gtttcgaggt ggttggtcag cgtcagaggc tggcgcacaa ccatgcggcc tgtttcacga
     1561 gcgaaatatt cgtcaaccgg cttgccgttg acaacgatct ttcccgttcc ctttttcagg
     1621 aacacacgag ccaccgacgt tttgcggcgg cctgttccat aattccaatt accgatcatg
     1681 agctatcctt agaattccag cggtttgggc tgctgagctg agtgaggatg ctcggcgccg
     1741 gcataaacct tgagcttctt ggccatggcg taacctagcg gccccttggg caacatgcct
     1801 ttaacggcct tttcgatagc gcgacccgga aaacgctgct gcattttttc gaaggtggtc
     1861 tcggtaatgc tgcccgggta cccggaatgg cgatagtact ttttagcttg cgccttatta
     1921 ccggttacaa cgatgtccgc tgcattgata atgacgatgt aatcgccggt gtcaacgtgg
     1981 ggcgtgaatt ctggcttatg cttgccgcgc aaacgacgtg cgacttcgct ggccacacga
     2041 ccgaggactt tacccctggc gtcaatcacg taccagtcac gtttgacttc atgcggcttg
     2101 gccacaaagg tcttcatgat ggttcctaaa caattaaaaa tacgcacgcc aaacatctgg
     2161 cgcgggctat atggtgtatt actcgtcccc atgcatgcct aagcgttgtg tctcgcccgt
     2221 gcacgcgcat ggtggaaaag ctgactatat taacatagtt ggccgttaat tcgcgcaacg
     2281 ccgctagcgg ctgccggcaa tggccatttg ctcgagcaaa atggaacctg tgcattttga
     2341 tccgcgcacg atgacgtcgg agccgaccgc tacaattcgc ttgaacatgt cggccaggtt
     2401 gccggcgatc gtcacctctt gcacggcgtg acaaatttcg ccgttttcaa cccaatagcc
     2461 gaaggcgcca cgcgaataat cgcccgtcac gtagttgacg ccctggccga ttaattcagt
     2521 caccagaagg cccattccca tttttttcag catggcgcga aggtcatcgg caggctgggt
     2581 gaggcgcgat gtcaaatcca ggttatgcga gccgcccgca ttgcccgtcg tccgcatctt
     2641 gagctttctg gccgtatacg ttgacaggaa atacccctgc aacacgcccc ctgtcacgac
     2701 ctcgcgtgac tgtgtgcgca ccccttcatt gtcaaacggg ctgcttccca taccggcgat
     2761 aatatgcggc ttttcagaga cgtcaagatg atcagacaag accggctggc caagcgagtc
     2821 aagcagaaaa ctggcttttc gatagagcgc cccgccactc gtggcctgaa ccagcgcgcc
     2881 aagcaagcca accgccagcg gagcctcgaa taaaaccgga aaatgacccg tcttgatgcg
     2941 gcgcgccgaa aggcgcgaca gcgtgcgctg tgcggcgtaa cggcctaccg cctgcgcgct
     3001 cgccaataac tcaggccggc gttgggcgct ataccaataa tcacgttgca ttgaggcgcc
     3061 gcgtcccgcg atgggagct
//
LOCUS       DATCXU010000025         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_937397,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000025 DATCXU010000000
VERSION     DATCXU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937397"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1798)
                     /locus_tag="VIR04_00540"
     CDS             complement(116..1798)
                     /locus_tag="VIR04_00540"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY9459265.1"
                     /translation="MGRLFTRYTPFYLTIFASLLFLIMAVSSSLLWLPLALFFIALSA
                     LGIADLTQTRHAIRRNYPVIGNLRFVLESIRPEIRQYFLEGDTQQLPFSRADRSLVYQ
                     RAKKEIDKRPFGTQRDVYSSDYEWINHSMDPTHPENSDFRITIGGPDCTRPYSSSVLN
                     ISAMSFGALSANAVLALNKGAALGNFAHDTGEGGVSRYHLANNGNLIWNIGSGYFGCR
                     DEHGNFSESIFARQATQDNIKMIEIKLSQGAKPGHGGILPGAKVTPEIARARGVTAWQ
                     DCNSPSRHSAFSTPIELMQFIVKLRELSGGKPVGFKLCVGHPWEWFAIAKAMLKTGIT
                     PDFIVVDGAEGGTGAAPIEFVDHVGTPLREGLRLVHNTLVGIGLRERIKLGASGKIIS
                     AFDMARTMALGADWCNSARAFMFAIGCIQAQVCHTGRCPTGVTTQDPKRQKALVVSDK
                     SVRVANFQQNTLKALAELLGAAGLAHPCDLRPHHIARRIPSGEVRVLSALFPDLEKGE
                     LLQGKFRQTIFRVGWPMAQAESFAPLYSLSAALSGNANSAIPSEVEPEEALA"
     gene            complement(1904..>2168)
                     /locus_tag="VIR04_00545"
     CDS             complement(1904..>2168)
                     /locus_tag="VIR04_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9459266.1"
                     /translation="PRVMLFDEVTSALDPELTEEVLAVIEQLARDGMTMLLVTHEMNF
                     ARRVANTIIFMNEGKIRETGEPEAMFSNPKTPEFELFVRRSFK"
BASE COUNT          451 a          608 c          629 g          480 t
ORIGIN      
        1 gcggcgtcgg cgacaacgtc ttcgccaagt tcgaatcttc agttttacgg gacgattgat
       61 gcgggcgtcg gcacgggaaa aacctggcgc tagaacgggc tcaaagtcgg cggccttatg
      121 ccagcgcctc ttcgggttca acttctgacg ggatggcact gttggcgttg cccgaaagcg
      181 cggcgcttaa gctgtagagc ggggcgaacg attcggcctg agccatgggc cagccaaccc
      241 tgaatatggt ctgccggaat ttgccttgta gcaactcgcc tttttccagg tcgggaaata
      301 gcgcagacag tacgcgcacc tcgccgctgg gaatgcgtcg ggcgatgtgg tgagggcgca
      361 ggtcgcaggg gtgggcgagg ccagccgccc ctaacagttc cgccagcgct ttcaatgtgt
      421 tctgctgaaa attggcgact cgaaccgact tgtcgctgac gaccagggcc ttttgacgtt
      481 tcggatcctg tgttgtgacg ccggttgggc atcggccggt atggcaaact tgcgcttgta
      541 tgcagccaat ggcgaacatg aaagcgcgcg cgctgttgca ccagtccgcc cctagcgcca
      601 tggtgcgcgc catgtcgaaa gcgctgatga tcttgcccga cgcccccagt tttattcgct
      661 cgcgcaaacc aatgcccacc agcgtgttgt gcaccagccg cagaccttcg cgtaacgggg
      721 tgcccacatg atccacgaat tcgatgggtg cggcgcccgt gccgccttcc gcgccgtcga
      781 ctacgataaa atcaggcgta atgcctgttt tcagcatggc tttggcgatg gcaaaccact
      841 cccagggatg gcccacgcat aacttgaaac caactggttt gccgcccgag agctcccgta
      901 gtttgacgat aaactgcatc agttcgatgg gtgtgctgaa ggcgctgtgc ctggagggcg
      961 aattgcagtc ttgccacgcg gtaacgccgc gcgcccgggc aatttcaggg gtaactttgg
     1021 cgccgggcaa gataccgccg tggcccggtt tggcgccttg cgacaacttg atctcgatca
     1081 ttttgatgtt gtcttgcgtt gcctggcggg cgaaaatact ttcggaaaaa ttcccatgct
     1141 cgtcccggca cccgaaatac cccgagccta tgttccagat caggttccca ttattagcca
     1201 gatgatagcg gctgacgccg ccttcgcccg tatcgtgggc aaagttgccc agcgccgcac
     1261 ctttgttcaa ggccagcacc gcattggccg acagcgcgcc aaagctcatg gccgaaatat
     1321 taagcactga tgacgaatag ggccgggtgc aatcgggccc tccgatggtg atccggaagt
     1381 cgctgttctc cgggtgggtt ggatccatcg aatgattgat ccattcgtaa tcgctactgt
     1441 agacgtcgcg ttgcgtaccg aatgggcgtt tgtcgatctc tttttttgcg cgctgataga
     1501 ccagcgatcg atcggcgcgg gaaaaaggca gttgctgagt gtcgccttcc agaaagtatt
     1561 gacgaatttc cgggcggatc gattcaagca caaaccgcaa gttgccaata acggggtagt
     1621 tgcgacgaat cgcgtgccga gtttgcgtca ggtcggcaat gcccagtgcg ctcaacgcaa
     1681 taaaaaagag tgcaagcggt aaccacagca ggctggaaga caccgccatg atcaggaaca
     1741 gcagagatgc aaaaatggtc aggtaaaacg gcgtgtaacg cgtgaacagg cggcccatag
     1801 ttctttttcc gaatgtttct tcgctttaca ctttacatcg tgcgccccgc ggcaacaaaa
     1861 ctttgtttgt gaacgccggg ttttattggc ccccgttcac gctctattta aagcttcggc
     1921 ggacaaacag ttcaaattca ggggttttgg ggttgctgaa catcgcctcg ggctcgcccg
     1981 tttcccggat cttgccttca ttcatgaaga tgatggtgtt ggcgacgcgg cgcgcgaaat
     2041 tcatctcatg cgtgacaagc agcatagtca tcccgtctcg cgccaactgt tcaatgaccg
     2101 ccagaacctc ctctgtcaat tcaggatcca gagccgaggt gacctcgtca aagagcatca
     2161 cacgcggt
//
LOCUS       DATCXU010000026         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_779036,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000026 DATCXU010000000
VERSION     DATCXU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779036"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VIR04_00550"
     CDS             complement(<1..749)
                     /locus_tag="VIR04_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459267.1"
                     /translation="MHRREFITLLGCAAAAWPLAAHGQQGERMRHIGALLPFSAEDAE
                     GKTIVTALRQGLEKFDWIEDRNIRIDYRFGGGDVVRTQAYARELVELSPEAIYAYLNG
                     QLAPLSHATRTIPIVFVGASDPVGAGYVESFARPGRNITGFILFEPSMVGKWLQALKE
                     LAPSVSQVAMMFNPETATRQGTFYSQEFGNAAAALSVESSIAAVRSANEIEAAISALG
                     QKPNSALIVASDTFTSVHGELIVTLAARHRLP"
     gene            complement(1339..1470)
                     /locus_tag="VIR04_00555"
     CDS             complement(1339..1470)
                     /locus_tag="VIR04_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459268.1"
                     /translation="MDRFTVFLIDDDIGTLTALSGFLRAAGYGLERMTLGLIIEDPK"
     gene            2098..2415
                     /locus_tag="VIR04_00560"
     CDS             2098..2415
                     /locus_tag="VIR04_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459269.1"
                     /translation="MASRLLIAQRFDPNGIIEMWHRHADPWALRGRLGAVAAVIIDGE
                     KKPTQPAKNSPPVQFSGEPNGAARNGQPISPRGVVEWRQTERKPARVMSQRGFLALKG
                     APG"
     gene            3001..3411
                     /locus_tag="VIR04_00565"
     CDS             3001..3411
                     /locus_tag="VIR04_00565"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEY9459270.1"
                     /translation="MVFKPGKSGNPSGKPKGARNKTTVAMEKLLDDDAATITSKAIEL
                     AKNGDLTALRLCLERIIPPRKDRPVNFDMPEIKTPSDALLAATAIMRAVAGGDLTPSE
                     AAELSKTIDSFVRVAETADLAERIKRLEQVADNS"
     gene            complement(4105..>4763)
                     /locus_tag="VIR04_00570"
     CDS             complement(4105..>4763)
                     /locus_tag="VIR04_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY9459271.1"
                     /translation="AITPDGKHAYFLGSADVLGSSSVSVLDIASNTAATTSLPNTRLL
                     EMAITPDWKHAYATGDGRVWVIETTTNTMVDTLTGQGPAAVATMPPSDSVIASCGVSV
                     FAQGFIGDFPQQGAATGQMRQFLGIPYAAAPVGANRWNPPQRFCWEGTRQFTAFGNHC
                     AQPPSPFGNLNFNEDCLFLNIFTPVNVDVNHPVPVMVWIHGGAFQFGESEAYNPVRLA
                     "
BASE COUNT         1109 a         1309 c         1353 g          992 t
ORIGIN      
        1 ggcaagcgat gccgggccgc gagcgtgacg ataagttcgc cgtggactga ggtgaaggta
       61 tccgatgcta cgataagggc gctattaggc ttctgcccga gagcagagat cgcagcctcg
      121 atttcgttgg cgctgcgcac agccgctatc gacgactcga ccgaaagtgc tgcagcggca
      181 ttaccgaact cttgtgaata gaaagtcccc tggcgggttg ccgtctccgg gttgaacatc
      241 attgcgacct gagacacaga cggcgcgagc tctttcaatg cctgcagcca ctttccgacc
      301 atcgatggtt caaaaaggat gaacccagta atgttacggc ctggacgcgc aaaactttcg
      361 acatagccgg cccctacggg gtccgaagcg ccgacgaaga cgatcggaat cgtgcgcgtc
      421 gcgtgcgata gcggagcaag ctggccattg agatatgcgt agatcgcctc aggcgacaat
      481 tctacgagtt ccctcgcgta ggcttgcgta cgcacgacat cgccgccacc aaagcgatag
      541 tcgatccgaa tattgcgatc ctcaatccaa tcgaacttct caagtccttg ccgcagcgcc
      601 gtcacgatgg tcttgccttc ggcatcttcc gcactgaacg gcaaaagggc tccgatatgc
      661 cgcatccgct cgccctgctg cccatgtgcg gcgagcggcc atgcggctgc cgcacagccg
      721 agaagcgtaa tgaactcgcg tcggtgcatg cgccctctaa ggaccacgtc ttgtgcaatg
      781 cccaaagcct agcactttgc gaccgggcgg cgagtgcaat atggcgctca accagcctcg
      841 aaactgatct ggccccatgt ccgctttggg tcataagcgg acattcgcac cgcaaaaggt
      901 catgtctgct ttatccctga aagccgacat ttgcagcgca aactgaaatg tccgctttgg
      961 gccaaaagcg gacattcgct ccgaaaaccg cctgcggatg tacgtttaga aaaggcctgc
     1021 aacgctggag gacgttaatg ttacttccct acgaaacgcg tcatcaaaat cagaataatc
     1081 atgcctagca ccacaagtga caaaacttct aatgagccat cgccacggcc actagaaaaa
     1141 ccgaaccatc tttcaaaaaa acccacggct ttctgcccca ggaaatttgt taccgcgagg
     1201 ctcagccggt ccagcaggtt tgtgaattgc acataggtat acggagtatt gcgttcctcc
     1261 gtcgctgcat gacgtgtgac cacacaccgc acatgtccgc tcttgtctgt tcaggaacga
     1321 ctagctggac atggcccact atttgggatc ttcgataatt agcccaagcg tcatcctttc
     1381 caacccgtag ccagcggctc gtagaaatcc gctcagtgcc gtcaatgtcc cgatgtcatc
     1441 atcgatgaga aaaactgtga aacggtccat gtgaaattca ttagatgtag tgaaaacaac
     1501 tgactgcgcc aaacaaaacg atgatgagaa ccaatgcgat taagaccgct cgcgtgtctg
     1561 ccttcatgaa gacgagacca aagttaaagc ccgcatcaac cccggcaatc gccgggactg
     1621 aagcaaaaag ctactgatca attttcaagc tgcttcgggg gagattagaa ttgtcggcaa
     1681 cccgacacta ttatccccga ggtcagaagg cctatcgcaa cgtcaggtga acctatcctt
     1741 tagtcgaggc cgtcttctcg gcgcgttcag tctcctgaca tagctgtcat tcgctgatcc
     1801 ttcttagcct gtgaactgag taacgtccaa aaacgccact gacaactgac atccctcact
     1861 gcagctatct tcgtgcgatg tccgctttgg gtcataagca gacatgtgca gtgcaccaac
     1921 caatgtccgc tttgccccca atagcgaccg cgaaagcgga catgaatttc tggacggaaa
     1981 ttttgtcggt cagtattgcc ccgcctgggg gatggcaggg cggggtctga ggttcaagtc
     2041 gaaacgcttt gctgaaccgg tttccccacg ctctgcattt ccgcatcgcc attggtcatg
     2101 gcgtcccggt tgctgatcgc ccagcggttc gatccgaacg gcatcatcga gatgtggcac
     2161 cgccacgccg acccttgggc gttgcgcggt cgcctcggcg cggtcgctgc cgttatcatt
     2221 gatggcgaaa agaagccaac ccagccagcc aaaaacagcc cgcccgttca attttcgggg
     2281 gagcccaatg gagccgcgcg caatgggcaa cccatttcgc cgcgcggcgt ggtcgaatgg
     2341 aggcagactg aacgaaaacc cgcgcgggtg atgagccagc gcggattttt agctttgaag
     2401 ggcgcgcccg gctgacagac cgattgagcg cggaagcagc gaagacaggt cggtggcttc
     2461 tgatcctgtc ctaacactgg cgacgattta atccctagtc gccggtgcaa tgagaggccc
     2521 acgtgtcggt gcgtttcacc ggtgatgata gcgcgggaga cctttctcat gggaaaccat
     2581 gaggtcccga tccgagcaaa tgccgcgtcg gctatgctcg tagctcaggc aatgttggca
     2641 tacagtaccc tctgcaccgg ggatattgat gcaggcccgt cgcctcgccc ccgacgccgc
     2701 tcatctgaaa tcgcgcgatt tttttcgcct tgagcgattt tgtagataac ggacctaaaa
     2761 agggcacctg aggtatacgc gctgaggcag agacgcaggg taacgtcccc aactggctcc
     2821 ccttgttcta aaggggccac cctttgggga cggggctatg ggaaaatgcg atgccacacg
     2881 atccggttta taactcgtcg cgctggcggc gcttgcgcag ggcgcggatg cacattgacg
     2941 gcggcgttgg ggggtggcgg tcattgaaaa tactatctgg aaacaaggga ccactgactg
     3001 atggtcttta aacccggaaa gagtggcaac ccctctggca agccgaaggg tgcgcggaac
     3061 aaaaccacag ttgcaatgga aaagctgctc gacgatgacg cggctaccat aacaagcaag
     3121 gcaatcgaac tagcgaaaaa cggcgacctg accgcgctgc ggctgtgcct ggagcggata
     3181 attcctccgc gcaaggatcg cccggtgaac ttcgatatgc cggagattaa aacgccttcc
     3241 gacgcgcttt tagcggccac agcaattatg cgtgcagtag ctggtggcga cctgacgcca
     3301 tcggaagcgg cagagttgag caagacaatt gatagctttg ttcgcgttgc agagactgcc
     3361 gatctcgcgg aacggattaa gcggctagag caagtggcgg ataatagtta ggggcgttcg
     3421 gtgcgggcat tgcaaacgag ggtgcaaaag cttgagcgcc gtagtggggt cgcgcagtca
     3481 ttctatattc tttgggtgcc cgccggcgca gatcgtgtat cggccctaga tcagctgcgg
     3541 gcgagtgaaa agattgctgc tgacgttccc gcttactgtg ccgagtggaa gtgtcgcggt
     3601 cgcgttctcg gaccgcgccc ccggtctcgt ttgactaacc gccgccacat tagcgatgac
     3661 gaagaggccg tgctgtttga agccatctgc cacgaccttg aatcgagcgg tttttccatc
     3721 agttccagtg caagcgacga tcttcgcggg gagcagtgca tgtgcgaaat gacgaaccgt
     3781 gaattgattg gggtcatact aaagagcccg atcgacatgt tggtctcagg gtcggctatt
     3841 cataagcgcc cagcaccacc gacgaatgtc cgctttcgcg gtcgctattg ggtgtaaagc
     3901 ggacatggcc ttttgcagtg cacatgtccg ctaatgaccc aaagcggaca tggcgcggtc
     3961 aaggcatcgc tcaagtcaaa tatgccagag tgcgatgtcc aaatacccag cagtcgaatg
     4021 ggtggctcaa tgagggcttg cgttagggat gaatgaaagc ggcggcgcga cagatactct
     4081 caaggtgctt ttttgcccgt ttgtctaggc gaggcggact gggttgtacg cctcgctttc
     4141 accaaattgg aacgcgccgc catgaatcca gaccatgacg gggacaggat gatttacgtc
     4201 aacgttgact ggcgtgaaaa tattcagaaa aaggcagtcc tcattgaagt taagattacc
     4261 gaaaggcgac gggggctgtg cgcaatggtt tccgaatgcc gtaaattggc gcgtgccctc
     4321 ccaacaaaat cgctgcggcg gattccaacg attggcgccc acaggcgcag cggcataagg
     4381 gatcccaagg aattgacgca tttggcccgt tgcggcgcct tgctgtggga aatctccaat
     4441 aaaaccttgg gcgaacacgg atacaccgca tgaggcgatg acggagtctg atggtggcat
     4501 cgtggcaacg gcagcgggtc cctgcccggt gagcgtgtca accatagtat tagtagtggt
     4561 ttctatcacc caaacacgcc catcacccgt cgcataggca tgtttccaat caggggttat
     4621 ggccatttct aacaaacggg tattcggcaa agacgtggtc gctgcagtat tgctggcgat
     4681 gtcaaggact gacacgctgg aggatccgag cacgtcggcg gatccgagaa agtaagcgtg
     4741 tttgccgtca ggagtaatgg cta
//
LOCUS       DATCXU010000027         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1009735,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000027 DATCXU010000000
VERSION     DATCXU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009735"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VIR04_00575"
     CDS             complement(<1..403)
                     /locus_tag="VIR04_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459272.1"
                     /translation="MTSVIVVEPHALLRFGILQHLNDVLPDSRIQGVDYSSLYGHASG
                     GGEIDLVLLSVAVHEDMAKLAKAAQRVYAPKRILLLSDAPTMPGSWRELPSIVAGYVP
                     RSASPDVLAASIRLVLVGGRCFQSYRRKPESA"
     gene            638..>2072
                     /locus_tag="VIR04_00580"
     CDS             638..>2072
                     /locus_tag="VIR04_00580"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY9459273.1"
                     /translation="MSNSFTTRPEIKGIFGVVSSTHWIASQVAMGVLEKGGNAFDAAV
                     AGGFSLQVVEPHLNGPGGEVPILFWSEKEKRIRSLCGQGPAPKLASTQYFRDKGLDLV
                     PGIGLLPACVPGAFGAWLTLLRDYGTWELEDILRPAIELASHGFPLVPRVVQAIVAVQ
                     DLFRKEWPDSAATWLPDGKIPDLNQLFRLPAIASTYTRVINEAKSRASGRRERINAAL
                     SIWQEGFVAQAIDRYYKTQSVRDTTGNRNSGLLRLEDMAAWKPAYEDPVTLQYGRYTV
                     AKCGPWSQGPVFLQQLSILRHFEVAKMDPNSAEFVHCLAETAKLAFADRLAWYGDPLA
                     VNVPLQALLSTEYSSERAKLIDATASKALRPGRPEGRDPILPDLKASERTLDSADVRF
                     GVGEPTFAELPPVETWAEKEIFVGDTCHLDVIDRHGNMVSATPSGGWLSSSPVIPELG
                     FPITTRLQITWLDDNVPGALRPGFRPTT"
BASE COUNT          473 a          578 c          588 g          433 t
ORIGIN      
        1 cggccgactc aggtttgcgc cggtatgatt gaaagcaccg tccgccaacc agcaccagtc
       61 tgatcgatgc agccaaaacg tccggcgatg cgctacgtgg cacgtagccc gctacgatgg
      121 acggcaattc cctccacgaa ccgggcatgg tcggcgcgtc ggacaacagc aatatgcgct
      181 tgggcgcgta aacacgttgc gccgccttgg ccagcttggc catgtcttca tgcacggcaa
      241 cagacaacag caccaaatca atttcgccgc cgccggatgc gtgtccataa agagacgagt
      301 aatccacgcc ctggatcctt gaatcaggca acacatcgtt caggtgctgc aaaatgccga
      361 agcgaagcaa ggcatgaggc tcgactacaa tgactgaggt catttttatt ccttttggca
      421 ggacacaaat cgtcataaat tttgtgtgaa taaacgttac agctttgtac gaaagtaacc
      481 atccgccgat tgacggataa cgcggggtgt ttgcgtgggc cgaaaagagg gtctgcgttg
      541 ccggcaaacc ggctggcagc ttgtactggg ccacatttgg attatgttaa atttgtagcc
      601 caacattcag cattaacatc actgtgggcc attgacgatg tcaaattcat tcaccacccg
      661 acctgaaatc aagggaattt tcggcgtcgt gtcctctaca cactggattg cgtctcaagt
      721 ggccatgggt gtgctggaaa aaggcggaaa tgcattcgac gcagcggtcg ccggcggctt
      781 ctcgttacaa gtggtcgaac cgcatctgaa tgggcccggc ggcgaagttc cgattttgtt
      841 ctggagcgaa aaagaaaaac ggatacggtc tttatgcggc cagggccctg ccccaaagct
      901 tgcaagcacc cagtatttcc gcgacaaagg cctggatctg gtgcccggca tcggccttct
      961 gcccgcctgt gtacccggcg cattcggcgc gtggctgacc ttgctgcgtg attacggcac
     1021 atgggaactg gaggatatcc tgcgtccggc aatcgaactc gccagtcacg gatttccgtt
     1081 ggttccccgc gtggtgcagg ccattgttgc cgtacaggac ctctttcgca aggaatggcc
     1141 cgattcagcg gcgacctggt tgcccgacgg gaaaattccc gatttgaatc aattgtttcg
     1201 actacctgcg atcgcatcga cgtatacacg agtgatcaac gaagcgaaaa gcagggcaag
     1261 cgggcggcga gaacgaatca acgccgcgct atcgatttgg caagagggat tcgtcgccca
     1321 ggcgattgat cgctactaca agacccagtc agtccgggac accacgggaa accggaattc
     1381 cggtttgctg cgcctggaag acatggcggc atggaagccc gcttacgaag acccggtcac
     1441 gctccaatac ggacgttaca ccgtcgccaa atgcgggccg tggtcgcaag gccccgtgtt
     1501 tctacaacag ctatccatat tgcgccactt cgaggttgca aagatggacc cgaattccgc
     1561 ggaatttgtg cattgtctgg ccgagacggc aaaactggcg tttgccgatc gactggcatg
     1621 gtatggcgat cctttggcgg ttaatgtgcc gctgcaagct ctgttatcca ctgagtattc
     1681 cagcgaacgg gcgaaactga tcgatgcaac ggcgtcgaaa gctttgcggc caggaaggcc
     1741 cgaggggcgc gatccgattc tgcccgatct gaaagcatca gagcgcactc tggatagcgc
     1801 agacgtgcgc ttcggcgtgg gcgaaccaac attcgcggaa ctgccacccg ttgaaacatg
     1861 ggccgagaaa gaaatttttg tgggtgacac ctgccatttg gatgtcatcg acaggcacgg
     1921 aaacatggtg tcggcgacac cttcgggcgg atggctgtct tccagcccgg tcattccgga
     1981 gcttggcttc cctattacca ctcgcttgca aattacatgg ctggacgaca acgtcccggg
     2041 agccctgcgg cctggattca gacccaccac ca
//
LOCUS       DATCXU010000028         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_306837,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000028 DATCXU010000000
VERSION     DATCXU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306837"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..566
                     /locus_tag="VIR04_00585"
     CDS             81..566
                     /locus_tag="VIR04_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY9459274.1"
                     /translation="MRRLPRQRPTENFSPADLTAIHWLGSDQHGAQVLTTARMLLSIE
                     QVTKKVLPPALAQACRVARIDEQRIILAVPSAAYASKLRQLAPRIAELLKASGWNLNE
                     IIVKVQAGLLQTRTKTTPPRQITPLDAKALNAFEELQNNLTPGPLADAVQRLLNHHRG
                     A"
     gene            complement(577..1500)
                     /gene="lpxC"
                     /locus_tag="VIR04_00590"
     CDS             complement(577..1500)
                     /gene="lpxC"
                     /locus_tag="VIR04_00590"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY9459275.1"
                     /translation="MLRQRTIQKPISTKGVGLHSGRRVEITLRPAAPNTGIVFHRVDL
                     PQVVDLPAQASHVGDTRMASVLQEGNVRVSTVEHLMSALAGLGIDNLHVDLTAEEVPI
                     MDGSAGTFVYLLRSAGLEEQLAPKQFLRVMKTVEVRDGDGEQLKWARLEPYDGFALSF
                     AIDFHHPAISSTANFAEIDFARDSYVKTISRARTFGFVSEVEALRAAGLARGGSLDNA
                     IVMDEYRVLNSDGLRYDDEFVKHKILDAIGDLYLIGKPLVGRYVACKSGHALNNQLAR
                     ALLARQDAWELVTYDSAIAAPKAFTNQWQLA"
     gene            complement(1712..>2201)
                     /locus_tag="VIR04_00595"
     CDS             complement(1712..>2201)
                     /locus_tag="VIR04_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY9459276.1"
                     /translation="AGADRARVAAEQAIACPLLEGVDLHGARGMLVNITASRTLKMRE
                     TREIMDTIRSYAAEEATVIFGTAYDENMGENLRVTVVATGLGRVDTASRPQLVQNNAQ
                     ALRNGTDNFPMIGGHQDFAGSDVPAVIRNPRGQASAQVHALETAGMDHYDIPAFLRKQ
                     AD"
BASE COUNT          544 a          628 c          571 g          458 t
ORIGIN      
        1 cattgcgctt agcacccaca tcgccgcgaa tagattttta ccgcgtttac tccacataca
       61 cttcccgctt gaaaatacgc atgcgccggc ttcccagaca acgacctacg gaaaattttt
      121 caccagcgga tttgacggcc atccattggc tgggcagcga tcagcatggg gctcaagttt
      181 tgacaacggc gcgcatgcta ttgtcaatcg agcaagtgac taaaaaggta ttgcctccgg
      241 cgctggcgca agcgtgccgg gtggctcgca tagacgagca acgaatcatc ctggccgttc
      301 ccagcgccgc ctacgcatcg aaactgcggc agttggcgcc gcgaatcgcc gaactactga
      361 aagcaagtgg atggaacctt aacgaaatta ttgtcaaggt acaagcgggg ttgttacaaa
      421 cgcggactaa aacgacacca cccagacaga tcacgccgct ggacgccaaa gcattaaatg
      481 ccttcgagga actgcagaat aatctgaccc cggggccgct ggccgacgca gtgcaacgtt
      541 tattgaacca tcaccggggc gcttaagccg tcacgctcag gcaagctgcc attgattggt
      601 gaacgccttg ggcgcggcaa tggccgaatc ataggtgacg agttcccagg catcttgccg
      661 ggcaagcaaa gcgcgggcca actggttgtt taatgcatgt cccgatttac acgcgacgta
      721 gcggcccaca aggggtttgc caatcagata taaatcgcca atggcatcaa ggattttatg
      781 tttgacgaac tcgtcgtcat aacgcaagcc atcgctattc aatacccgat actcatccat
      841 gacaatggcg ttgtccagac tgcctccacg cgccaggccc gctgcgcgca aggcctcgac
      901 ttcactaaca aagccaaagg tgcgcgcccg tgaaatcgtc ttcacatacg aatccctggc
      961 gaaatcgatt tcggcaaaat tagccgtgga actaattgcc ggatgatgaa aatcgatggc
     1021 aaacgacaac gcaaagccgt catagggttc cagccgcgcc catttaagtt gttcgccgtc
     1081 gccgtcgcgc acttcaactg ttttcatcac ccgcaggaat tgtttggggg ccaactgctc
     1141 ttcaagtccg gcggaacgca gcaaataaac gaatgtcccg gcgctgccgt ccatgatcgg
     1201 gacttcctcg gcagtcaaat cgacatgcag attatcgatg cccaatcccg ccaacgccga
     1261 catcagatgc tcaaccgtcg aaacacgcac gttgccttcc tgcaaaaccg atgccattcg
     1321 agtgtcgccg acatggcttg cctgagcggg caaatcaacc acctggggca gatcgacgcg
     1381 atgaaacaca atgccggtgt tcggcgccgc cggacgcaag gtaatttcga cgcggcggcc
     1441 agaatgcaaa ccaacaccct tggtgctgat tggtttttga atagtgcgct gacgtaacat
     1501 gatggtgaaa agggatccga attcgtgcga ttcacgcggt taaattacca tgatttaaat
     1561 atccgtatta acccttattg taaccaagtt aatatttacg gatctcgggc gcgcaagagc
     1621 cccatcctcc cggggaaaaa ggaagatggg gacaattgaa gtctaatcag acaacaatga
     1681 cgcctttgcg gacatgtgag agtccgtttt tttaatcggc ctgcttgcgc aaaaatgccg
     1741 gaatatcgta atgatccatg cccgccgtct caagcgcgtg cacttgagcg gaggcttggc
     1801 cacgcggatt gcgaattacg gcgggaacgt cggagccggc aaaatcctga tgacctccaa
     1861 tcatcgggaa gttatccgtg ccgttgcgta gcgcttgcgc gttattttgc accaactgag
     1921 ggcgcgatgc ggtgtcaaca cgacccaaac ctgttgccac aacggtaaca cgcaagtttt
     1981 cgcccatgtt ctcgtcatag gctgtaccga aaatgacagt cgcctcttcg gccgcataac
     2041 tgcggatcgt atccatgatt tcgcgcgttt cgcgcatttt cagggtacgg ctggccgtaa
     2101 tgttcaccag catgccgcga gcgccatgca ggtcaacgcc ttcaagcaag gggcaggcaa
     2161 tagcctgctc agcggcaacc cgcgcacggt ccgcgccagc c
//
LOCUS       DATCXU010000029         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1086675,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000029 DATCXU010000000
VERSION     DATCXU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086675"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..718
                     /locus_tag="VIR04_00600"
     CDS             521..718
                     /locus_tag="VIR04_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459277.1"
                     /translation="MQQYKMSFLDKLNFVVTVRNFDADTDTDALTHAYSLCGTHTINV
                     TQADRRVGEVSKGALAETCIA"
     gene            884..1648
                     /locus_tag="VIR04_00605"
     CDS             884..1648
                     /locus_tag="VIR04_00605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY9459278.1"
                     /translation="MSSLLLQNAYLTAMLKQAGLDAEARDVAQRIQSVLTDEIHHRMK
                     NMLTMVTAIVRQSMRSATNLVDAEKAISTRLIAMAKAHDMLLKADLKSADLASVIAGA
                     VAQHNSALGAIDIMGDAMQVRPSAILPLTLILNELCTNATKYGALSVPGGSVALRWTQ
                     DAERGAMVLHWAEMNGPLVRPPTTRSFGSRLLEEAIPRQLGGFGRLSFPASGVAFNLT
                     VPLDKLQPDGLAENNSASSHIARTGAHAHATHSDST"
     gene            1623..1820
                     /locus_tag="VIR04_00610"
     CDS             1623..1820
                     /locus_tag="VIR04_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459279.1"
                     /translation="MQRIPTALELSTDEFLSMREIVARSFIPANQIGQRTKARLIELG
                     LVQKALGGLMATPAGRIVARL"
     gene            complement(2670..>2886)
                     /locus_tag="VIR04_00615"
     CDS             complement(2670..>2886)
                     /locus_tag="VIR04_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459280.1"
                     /translation="GSSMMDSSAPPERLGVVQRAFEIAKSGAVADIAALNAQLAAEGY
                     ADSVQILAGRSLQQQLLRMILEAVRSR"
BASE COUNT          663 a          834 c          826 g          563 t
ORIGIN      
        1 caacgaagga aagcgtctcc agcatctttt cctcggtcct cactgtgtac gtgccgggcg
       61 gctggatacc atccagcccc gaaagcgtga aaggtctgcg aaacgtaaca agtttgctgg
      121 tggtacggtt ttccatgacg ccttcctgca agacagcaac agccgggcgg cgtttccacc
      181 gcccggccgc gccccggcgc gatgatagcc caaatcacgc atacgcgata cgcgaacgca
      241 tcagcaagcg tgagggctta aaaagcgcca ggagtctgtg acgtatcgga atgagaggca
      301 gcgaagatca ttgccactag cagtaaatgg tgcgccggac acgcaaatac aagtcaggca
      361 gcacaaatga atttgtgcgt gcgattaggt aacaatgatg agggtatgct acaagctgcc
      421 gtcgctggct gccgatacga gcgctggcgc ttgagagacc tccgggacaa ttcacagaac
      481 actgtccgcg ctcccgctcg cttttgggag taacctgctg atgcagcaat ataagatgtc
      541 ctttctggat aagctgaatt ttgtcgtcac agtgcgcaac ttcgacgccg atactgacac
      601 cgacgcactg actcatgcct attcgctgtg cggaacccat acgataaatg tcacccaggc
      661 cgaccgtcgc gtcggtgagg tctccaaggg cgcgttggcc gagacctgca ttgcctaagc
      721 ggtggggccg cattttcacc tggcgaaggc gcgctcgtgg cgtcgtttct gtggcacaac
      781 atcaggcgcc gatttttccg ccaacggaaa ccgctgcccg caacatcgct cgcatgcgtg
      841 gcgcggcgat ggcgcttgaa cgccgcatga tcgacaagcg ataatgtcgt cgctacttct
      901 ccagaacgcc tatctcaccg cgatgctgaa acaggcggga ctggacgctg aagctcgcga
      961 cgtggcgcag cgcatccagt ccgtcctcac cgacgaaatc catcaccgca tgaagaatat
     1021 gttgacgatg gtgacggcta tcgtgcgcca gagcatgcgt tccgccacca acctcgttga
     1081 tgcggaaaaa gcaatctcca cccgcctaat cgccatggca aaggctcatg acatgctgct
     1141 gaaggcagac ctgaaatccg ccgatcttgc gagcgtgatc gcgggggcgg tagcgcagca
     1201 caacagcgct ctcggcgcca tcgacattat gggcgatgcg atgcaggtga ggccctcggc
     1261 aatcttgccc ctgaccctga tcctcaatga actctgcacc aatgccacaa aatatggtgc
     1321 cctgtcggta ccgggcggca gcgtggcgct gcgctggacg caggacgcgg aacggggcgc
     1381 aatggtgctg cactgggcgg aaatgaatgg ccccctggtg cgtccgccga ccacccgcag
     1441 cttcggttcc cgcctgctgg aggaagcgat accgcgccag ctaggcggct tcggccgatt
     1501 gtcctttcct gcttctggcg tcgcattcaa cctgaccgtc ccgttggaca aattgcagcc
     1561 cgacggcttg gcggagaaca attccgcgtc atctcatatc gcgcgcactg gagcgcatgc
     1621 ccatgcaacg cattccgaca gcacttgaac tgagcacgga tgaatttctg tccatgcgcg
     1681 aaatcgtggc gcgcagcttc atacccgcga accagattgg tcagcggaca aaagcgcgac
     1741 tgatagagct cggtctggtt cagaaagcgc tgggaggcct catggccacg ccggccggac
     1801 gcattgtggc gcggttataa cgcgtctgcg ttcgatgctt ttgaatgtcc tatcgttggc
     1861 ccaaagggag gttagtaggt tcagacaaca ctaaattacc gactgcacgc ttgttcataa
     1921 gcgcacaagg gagtaggcgc gcagggctac atcgacaacg tgcagtaact tgcaattaac
     1981 agcagggaga agccaaatga acaaagaact gaataaccat cttgtaaaaa ggaaacccag
     2041 gcaggatagg aatacgaagc gcgcaaagag cggtgtggct tggcagcaca ctgtttatgc
     2101 gtagatccct gcttccaaaa cggtggccat tccatcggag ggcacgtgct ccgatggttc
     2161 ttatgagcgc ggctagccct ccacggtatg ccctgcaaac ttacaatggc caccaactcg
     2221 aaattaaatc tctcgccatt ggttggcagg taataattac ccgggacgga gcatatgtaa
     2281 gtaacgggaa tgtagaagga agtcttccga ctgcgctgga ggaggcccac aaatccatcg
     2341 acgtccaggt ggccgagccg agcccgttaa attaagtctc ctatctgtgt cacactcaag
     2401 cgaaacaatg cgcgggtatt ctaatcattt cttcagaccg aagccgcggg gaaattcgcc
     2461 gggacggtcg gtatattcgg ggtgaagatg cttaggggcg gtcagcacga agcgccgcga
     2521 tacccgtctc agccacgcaa accggacctc gctgtaaaac cgcactgggc gccatgcgaa
     2581 ccagggctgc cactcgctga cattgtgcac atcccggaca aagttaccgc gcggcagcct
     2641 tccgaaaggc gctttgtatt tggaggaatt catctgctgc gcaccgcttc aagaatcatt
     2701 cgaagcagtt gctgctgaag agaacggcct gccaggattt ggacgctatc cgcatagccc
     2761 tctgcggcaa gttgcgcgtt caaggcagct atgtccgcga ctgccccgct tttggcgatc
     2821 tcgaaggccc gttggacgac gccgaggcgc tcaggcggcg cactggaatc catcatggag
     2881 ctgcca
//
LOCUS       DATCXU010000030         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_222736,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000030 DATCXU010000000
VERSION     DATCXU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222736"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VIR04_00620"
     CDS             complement(<1..579)
                     /locus_tag="VIR04_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459281.1"
                     /translation="MTRNKAFWLLIACIAAVLYPLIFNTPFLQRLGALVLLYAIAASA
                     WNIVGGYAGQISFGHVIFFGCGAYSSLAMFQHFGLPPIVGLPIGILASVLIALVIGVP
                     TLRLSGHYFSMATIAVAELIRLVVTNTEYLGAALGLMGTAMSRTVFDLTFKSALPYYY
                     IFLGVLVITLLVTWWIGRNRIGFYLRAIKDSER"
     gene            complement(576..1442)
                     /locus_tag="VIR04_00625"
     CDS             complement(576..1442)
                     /locus_tag="VIR04_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459282.1"
                     /translation="MSLILLQVIIGGLLLGAVYALFSSGLTLVWGMMNVINFAHGDFV
                     MLGMYVAFIMWQVLGMGPAAAVPVAALALATLGVIVYFTLIRHVMKGPMIAQILGTFG
                     LALLLRYSAFWYFGSNFQSLPNDIVGGTFDLGGIRIEASRLLAGGVALAVMAALHLLL
                     TKSRIGSRMMAVSEDASAAQLMGIRPEVMQALAWGIAGAATGIAGALIANFFYVSPTV
                     GESLVIIAFVTVSLGGFGSIPGALIAGLLIGLIESLSSYLIGPVYKDIVIYSLFVAVL
                     WLRPQGLMGEKS"
     gene            complement(1453..2703)
                     /locus_tag="VIR04_00630"
     CDS             complement(1453..2703)
                     /locus_tag="VIR04_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459283.1"
                     /translation="MFKSKTLGRVAGALALAACFVGSAYAQDTVKVGVILPFSGNAAS
                     VGTAGKAAIELALDIINNPHPELGDLLLAKGTGLPGLKGAKLAIAFADNQGSPAVGQN
                     QALHLITEEKVSALMGAYQSGITNTASAVAERYGVPFVNGTSTAANLTERGFKWFFRT
                     TPIATDIAKVYVDFLQDLKSKGKKVDSIAIVHENTEYGTSIAKVLNSSAKASGLNISM
                     VIPYSANTADVQAPVLQLKEKKPDVVIFVSYTSDAILFAKTMKSLDYKAPIIIGDDSG
                     FSDPSFVKSAGSVTEGILNRSSWSAGKPGSATYLINEMYKKKTGHGLDDSSGRFMQAF
                     FVMADAINRAGSTDAAKIQAALRATDIKPNQLMMGYKGVKFDDKGNNILAAAVLIQLQ
                     KGEYVEVWPEANAMAPLQLPYKGW"
     gene            3072..3854
                     /locus_tag="VIR04_00635"
     CDS             3072..3854
                     /locus_tag="VIR04_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9459284.1"
                     /translation="MELEIPGYKIDILVQGYPGKTVCHGALGWSTVALVRSSEQAIIV
                     DTGSFGIRQVLIAQLKQRGLTPADVSCVLLSHAHYDHAVNWTMFPNAKIVIGAAELDW
                     SLRQPWGTTFVPELYVRELKSWPALHTAGDGEEVLPAITAHLAPGHTPGHLIYVLKTR
                     DIDVVFSGDAAKNRAEIISGQGFHTYDAAVSSASIEMIRKLWGRRKGSILVPGHDLPM
                     VRDGSEIRYLGERDASVEAWLGETLDQTTSFSLSLPSEAALR"
     gene            complement(3902..4792)
                     /locus_tag="VIR04_00640"
     CDS             complement(3902..4792)
                     /locus_tag="VIR04_00640"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY9459285.1"
                     /translation="MNPLQGLCAFPITPTDQHGSVDTQALGALISRIRTARADSICVL
                     GSTGTYPFLTRAERRRTIEFAVSQAGTTPIFAAIGALRTDEVIALGRDAQEAGVSAAL
                     LAPVSYTPLTDREVFTLFETVNAALDIPICIYNNPGTTHFTFSNQLIGRLNKLAHVMG
                     AKKPAPAPDGIVDNLADLRGKAAPGFSLGYSKDWYATEALIAGADAWYSVIAGLFPEA
                     CVRIVSAIKAGDLKLARSLNGQLEPLWTLFKELSSLRVMYAAASHSGLVHAAPPLPIL
                     PLADNDRQRVETVLDRLQLR"
     regulatory      4874..4971
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1172 a         1372 c         1445 g         1080 t
ORIGIN      
        1 gcgctctgaa tctttgatgg cgcgcaaata gaagccaatt cggttgcgcc cgatccacca
       61 ggtgaccagc agcgtaatga cgagcacacc aagaaaaata tagtagtaag gaagcgccga
      121 tttaaaggtc agatcgaaca cggtgcgcga catggccgtg cccatcaggc ccagcgccgc
      181 gccgaggtat tcggtattgg taaccaccag gcggatcagt tcggccaccg caatcgtggc
      241 catgctgaag taatggcccg ataaacgtaa cgttggcacg ccaattacaa gggcgatcaa
      301 cacgctggcc agaatgccga tcggcagccc cactatcggg ggcaggccaa agtgctgaaa
      361 catggccaga gagctatacg ccccgcagcc aaaaaagatg acgtggccaa acgagatctg
      421 gcccgcgtat cctccgacaa tgttccatgc cgatgccgct atggcgtaca acaaaaccag
      481 cgcgcccagc ctttgcagaa acggcgtatt gaaaatgagc ggatataaaa ccgctgcaat
      541 gcaggcaatc aacagccaga atgccttgtt ccgcgtcatg atttttcgcc catcagaccc
      601 tgtggacgga gccacagcac agcaacaaag agcgagtaaa tgacaatatc cttgtaaacg
      661 ggtccgatca gataggacga cagagactcg atcaggccga tgagcaggcc cgcgatcaaa
      721 gcgccgggga tgctgccgaa accgcccagc gatacggtta cgaaggcgat gatcaccaga
      781 gattcgccca cggtgggcga tacgtagaaa aagttggcaa tcagcgcgcc tgcaatgccg
      841 gtggccgcac ctgcaatacc ccaggcgaga gcctgcatta cttccggccg aatacccata
      901 agctgtgcgg cggaggcgtc ttcggaaaca gccatcatgc ggctgccaat gcgtgatttg
      961 gtcagaagaa gatgcaaggc cgccatcact gccagcgcga cgccacccgc aagcaaccgg
     1021 gacgcttcga tgcggatccc tcccaagtcg aacgtgccgc ccacgatgtc gttcggcagg
     1081 gactgaaaat tcgatccgaa ataccagaac gcggagtagc gcaacaacaa ggcaaggccg
     1141 aaagtcccca gaatctgggc gatcatgggg cccttcatga cgtgccggat cagggtgaag
     1201 tagacgatga cgcccagcgt ggccagcgcc agtgcggcga ctgggaccgc cgcggccgga
     1261 cccatgccca aaacctgcca catgatgaag gccacataca tgcccagcat gacgaaatcg
     1321 ccatgagcaa agttaatgac attcatcatg ccccagacca aagtgaggcc cgaggagaac
     1381 agcgcataca ccgcgcccag cagaagaccg ccgataatga cctgaagcag gattaaggac
     1441 atagagcttt aattaccacc ctttgtaagg gagttgaagc ggcgccatag cgttggcctc
     1501 tggccagact tcgacatatt cgcctttttg aagctgtatc agcacggctg ccgccaggat
     1561 attgttgccc ttgtcgtcga atttcacgcc cttgtaaccc atcatcagtt gattcggttt
     1621 aatgtcggtg gcccgcaatg ccgcctggat ttttgcggcg tcggttgaac cggcccggtt
     1681 gatggcgtcg gccataacga agaaggcttg catgaaccgg cccgatgaat cgtcaagacc
     1741 atggcccgtc ttctttttgt acatttcgtt aatcaggtac gtggccgagc ctggtttacc
     1801 ggcgctccat gaggaacgat tcaatatgcc ttctgtgacg gaacccgccg atttcacgaa
     1861 tgacggatct gaaaaaccgg agtcgtcgcc gattatgatg ggcgccttgt aatcaagcga
     1921 tttcatggtt ttcgcgaaca gaatcgcgtc ggatgtatag ctcacgaaaa tgacaacgtc
     1981 aggcttcttc tctttcaatt gcaataccgg cgcctgtaca tccgccgtgt tggcgctgta
     2041 ggggatgacc attgaaatat tcaagccgct ggccttggcc gaactgttta gcaccttggc
     2101 gatggaggtg ccgtattctg tattttcgtg aacgattgcg atcgaatcca ccttcttgcc
     2161 tttcgacttc aggtcttgca ggaaatccac gtagaccttg gcgatgtcgg tggcaatagg
     2221 cgtcgttctg aagaaccatt tgaacccgcg ctcagtcaag ttggctgctg tggatgtccc
     2281 gttcacgaac ggaacgccat agcgttctgc gaccgcgctg gccgtgtttg taatgccgga
     2341 ttgatacgcg cccatcaagg cgctgacttt ttcctcggtg atcaggtgca acgcctgatt
     2401 ctgaccaacg gcgggcgaac cctgattgtc ggcgaaggct atcgcgagtt tggcgccctt
     2461 gagtccgggc aggcctgttc ccttggccag aagcaaatcg cccagctcgg ggtgagggtt
     2521 gttgatgatg tccagcgcta attcgatggc ggcttttcca gccgtgccca cgcttgcggc
     2581 atttccgcta aagggaagga ttacacccac tttcactgtg tcctgggcgt aggcggagcc
     2641 gacgaagcag gctgccaaag ctaatgctcc ggccacgcgg ccaagcgttt tgcttttgaa
     2701 cattgcacat ctcctttgat tatttctgaa aaccattaaa cgaacagcat gggcaggcga
     2761 aaaatttcct gcggttgcag accgtattag aaagcctgat gggttggaac aggcgccccc
     2821 actgtaggtg tatgttgtgt tttttttaag actggcctga cgccgtggcg tcacgagttt
     2881 cggcgtacca cgcaccggct tgaaagcccc tggaacgccg caatcgcgct aagtatagga
     2941 gtaaatagtt tgtccggtca aacattccca tctgaagttt ggcctgtaat cagagccgag
     3001 ctgttttaga atggcgaagg tagaataaga ttcccgcaag gggaaaagcg cggcttaaca
     3061 agggataaga catggaactg gaaattccag gctacaagat cgatattctg gtgcaaggat
     3121 atccaggcaa gacggtctgt cacggtgcac tggggtggag cacggtggcc ttggtgcgaa
     3181 gctctgaaca ggccatcata gtcgatacgg gctcattcgg catacgccag gttttgattg
     3241 cgcaattaaa gcagcgcgga ctgacgcccg ccgatgtgtc ctgtgttttg ctcagtcacg
     3301 cgcattacga tcatgccgtc aactggacaa tgttcccgaa tgccaaaatt gtgatcggcg
     3361 ccgccgaatt ggattggtct ttgcgccagc catggggtac gacgtttgta cccgagttgt
     3421 atgtgcgcga acttaaaagc tggccggcat tgcatactgc aggcgacggc gaggaagtgc
     3481 ttccggccat aacggcgcac ttggctcccg gccatacacc cgggcatctg atctatgtgc
     3541 tcaaaacccg cgacatagat gtcgtatttt caggcgatgc cgccaagaat cgcgccgaaa
     3601 ttatcagcgg ccaggggttc cacacatacg acgcggcggt gagctcggcg tcgatcgaaa
     3661 tgatcagaaa attgtggggt cggcgcaaag gaagcatcct ggttccgggc cacgaccttc
     3721 ccatggtgcg cgacggcagc gaaatccgct atcttggcga acgcgatgcc tccgtggaag
     3781 cctggttggg cgaaacattg gatcaaacga cttcgttcag tctttcgctg ccgtctgaag
     3841 cggcgttgcg ctagtcaagt tgcagtgctg cgcgaggcga caagttattt cgcgcccagc
     3901 gttaacgaag ctgcaatcgg tccaggacgg tctccacacg ctgccggtca ttgtctgcaa
     3961 gaggaaggat aggcagcggc ggcgcagcgt gcaccaaacc tgaatgcgat gcggccgcat
     4021 acatgactcg aagactgctc aattctttga aaagagtcca aagcggttcc agttggccgt
     4081 tcaatgaccg agccaatttc aaatcgccgg cttttatcgc gcttacgatg cgtacgcagg
     4141 cttcggggaa cagaccggcg atcacgctgt accaggcatc ggcgccggca atcagcgctt
     4201 ctgttgcata ccagtctttg ctgtagccca gcgaaaatcc cggggcggcc ttgccgcgca
     4261 aatcggccag gttgtcgacg atgccgtctg gcgcgggggc gggtttcttg gcgcccataa
     4321 cgtgtgcaag cttgtttagc cggccgataa gctggttgct gaaggtgaag tgagttgttc
     4381 ccgggttgtt ataaatgcag attggaatgt ccagcgccgc attgaccgtt tcaaatagcg
     4441 tgaacacttc acgatccgtt agcggcgtgt aggaaacagg agccagtaga gccgccgata
     4501 cacccgcttc ttgcgcatcg cgccccagcg ctatcacttc gtcggttcgc agggcaccga
     4561 tggcggcgaa gatgggcgtt gtgcccgctt gcgacacggc gaattcaatt gtgcgacgcc
     4621 gttcggcccg cgtaaggaat gggtaagtgc cagtgctgcc aaggacgcag atcgagtcgg
     4681 cccgcgcggt gcgtatacgg ctgataagtg cccccagagc ctgagtgtcc acgctgccat
     4741 gctggtcggt tggcgtgatc ggaaaagcgc atagtccttg tagcggattc ataattttgt
     4801 tcctttttcg acagcgatac ggtaacgcac aaaattccaa ggttattgta aattttcagc
     4861 atcggctata gttgcgctta tgcaggggta ctcgtcgagg ttgacgggtt gagaaaaacc
     4921 ctttgtacca gtacggataa tgccgatgct gggagcgcgc tcaagggttc cagcggatcc
     4981 caagatctat ccccactcgg ctccatttgt ttacttttgg agcatgtctt gaacatccat
     5041 tttgaatttc cggccgtccc ggcgcggat
//
LOCUS       DATCXU010000031         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_66380,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000031 DATCXU010000000
VERSION     DATCXU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66380"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIR04_00645"
     CDS             complement(<1..202)
                     /locus_tag="VIR04_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9459286.1"
                     /translation="MPEAALLLDNITCTFVSHHDGQQGYTAVKETSLAVAPGEFVSVV
                     GPTGCGKSTLLNVGAGLLAPTSG"
     gene            complement(195..1235)
                     /locus_tag="VIR04_00650"
     CDS             complement(195..1235)
                     /locus_tag="VIR04_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459287.1"
                     /translation="MSLSRRDVLKMAGVAGAATVAPIFARAQGLEKKEVLVAVGGQAL
                     IYYLPLSIASLKGYFKDEGLDVKILDFAGGSKALQAVVGGSADVVSGAFEHTLTLQSK
                     GQMYRTFVQQGRAPMIVLGVSTKTMAGYKSPLDFKGKKIGVTAPGSSTNMMASFYLSK
                     HGVKPTDVSFVGVGAGAGAVTALRSGQIDAMSNLDPVISTLVHANELKIVADTRTLKD
                     TKEIFGGNMPSGCLYAAQQFLDKNPNTAQALANAIVRADKWIQASSFDEIAKVVPKNY
                     LLGDVELYKLALKGNKEALSPDGMVPEDGPQTALKALAAFIPDFPRDKIDTSKIWTND
                     FVIKANKKYPHA"
     gene            complement(1385..1993)
                     /gene="recR"
                     /locus_tag="VIR04_00655"
     CDS             complement(1385..1993)
                     /gene="recR"
                     /locus_tag="VIR04_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY9459288.1"
                     /translation="MDAPVTEPEPLKALIDALRRLPGIGVRSARRIAYHMLQYDVSGA
                     DQLSHALADAVRLLRHCTRCNSFAESELCSICSNPKRDPSILCVVETPVDQNMLESSQ
                     GYRGLYYVLMGRLAPLEGVGPQDLQFDRLLDRASDGIVQEVILATNFTAEGETTAHYL
                     SELFRARGLTVTRLARGVPAGSELEYVDAGTIAWALMERRKS"
     gene            complement(1993..>2224)
                     /locus_tag="VIR04_00660"
     CDS             complement(1993..>2224)
                     /locus_tag="VIR04_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY9459289.1"
                     /translation="EGASGGALVTVSMTCRHDVKRVTIDPSLLTDDKDMLEDLVAAAF
                     NDALRKAEAASQEKMAAVTAGMPLPPGMKLPF"
BASE COUNT          528 a          637 c          584 g          475 t
ORIGIN      
        1 ggcccgatgt gggagccagc aagccggccc ccacattaag cagggtcgac ttgccgcaac
       61 ccgtaggccc gacaaccgat acgaattcgc ccggagcaac agccagcgat gtctctttga
      121 ccgcggtata gccctgctgg ccatcgtgat gcgaaacaaa ggtgcaggta atgttgtcaa
      181 gcaacaaggc ggcttcaggc atgaggatat ttcttattgg ctttaatgac gaaatcgttg
      241 gtccagattt tggatgtatc gattttatcg cgcgggaaat cagggatgaa tgcagccaaa
      301 gctttcaggg cagtctgagg gccgtcctcg ggcaccatgc cgtcaggcga caaggcttct
      361 ttattgcctt tcagcgccag cttgtaaagt tcaacatcgc ccagcaaata gtttttcggc
      421 acaactttgg caatttcgtc aaaactggac gcctgaatcc atttatcagc acgaacaatc
      481 gcgttagcca aggcctgagc ggtgtttggg ttcttgtcca gaaattgttg cgccgcgtac
      541 aggcatcccg aaggcatgtt gccgccaaaa atttctttcg tgtctttaag cgtgcgcgta
      601 tcggcgacaa tctttagttc attagcgtga accagcgtgc tgataaccgg atcaagattg
      661 gacatggcgt caatttgccc gctacgcaag gcggtgaccg cgcccgcccc ggcgccaacg
      721 cctacgaagg acacatcggt cggttttacg ccatgcttgg agagatagaa gctggccatc
      781 atgttcgttg atgagccggg cgcagtcacc ccaattttct tgcctttgaa gtccagaggc
      841 gacttgtagc cggccatggt tttcgtcgac acgcccagca cgatcatggg cgcccggcct
      901 tgctgcacaa aagtgcgata catctgcccc ttggactgca acgtaagtgt atgctcgaag
      961 gcgcccgaca caacgtctgc gctaccgcca accacagcct gaagcgcttt ggagccgccg
     1021 gcaaaatcaa gtatcttgac gtcgagccct tcgtctttaa agtaaccctt caggctcgcg
     1081 atggatagtg gcaggtaata aataagagcc tgtccgccta ccgcgacaag cacctctttt
     1141 ttctcaaggc cttgtgctcg cgcaaaaatg ggggccacag ttgccgcgcc ggcaacgcca
     1201 gccattttaa gtacatcacg acgggataaa gacataagag acctcctaag tcacaacatt
     1261 ataaaaaggg caaacaggca gtccgccatt gttcgtttat ttgcaattac tctcgctttt
     1321 atattacccg agaagccggc tggtgcttat tggttattgc cccataggct tcaaaagacg
     1381 caagtcagct tttacgccgc tccatcagtg cccaggcaat tgtgcccgca tccacatact
     1441 cgagctcgct gccagccggt acgccgcgcg ccagacgagt gactgtcaac ccgcgggcgc
     1501 gaaaaagctc ggacagataa tgggcggtcg tctcaccctc agccgtaaaa ttagtggcca
     1561 aaataacctc ctggacgatg ccgtccgacg cgcgatcgag caagcgatca aattgcaagt
     1621 cctgcggccc tacgccttcg agcggagcca aacggcccat aaggacataa tacaaacccc
     1681 gataaccctg acttgattca agcatatttt gatcgaccgg cgtttcgacg acgcacagaa
     1741 tcgatggatc ccgcttggga ttggagcaaa tggaacataa ctcgctttcg gcgaaactat
     1801 tgcaacgcgt gcaatggcgc aaaaggcgaa ccgcgtcagc caatgcatgg cttaattgat
     1861 cggcgcccga gacatcgtac tgaagcatat ggtaagcaat gcggcgcgcc gaacgcactc
     1921 caatgccggg caagcggcgc agagcgtcga tcagcgcctt cagaggttcg ggttccgtca
     1981 ccggagcatc catcagaacg gcagcttcat ccccggaggc aacggcatgc ctgccgtcac
     2041 ggcggccatt ttttcctgcg aggcggcttc tgccttgcgc aacgcatcgt tgaacgcggc
     2101 agcaacaagg tcttccagca tgtctttatc gtcggtcaat aagctgggat cgatagtcac
     2161 gcgtttgacg tcgtgacggc atgtcatcga aacagtgacc aaagcgccgc ccgacgcccc
     2221 ctcg
//
LOCUS       DATCXU010000032         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_539992,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000032 DATCXU010000000
VERSION     DATCXU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539992"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIR04_00665"
     CDS             <1..535
                     /locus_tag="VIR04_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEY9459290.1"
                     /translation="RAGGTLEMVQLWVNLPAKDKMAPPGYQTLLESDIPSVDLPDGAG
                     RVRVIAGKYDGHEGPAKTFTPLDVWDLRLLQGKTASLTVPEGRTFALVILHGTVLING
                     EHIAREAQLVTLDSAGTAVSLEANNDATILALSGEPILEPIVGYGPFVMNSQAEIQQA
                     IQDFNSGRFGQMSASAS"
     gene            556..1479
                     /locus_tag="VIR04_00670"
     CDS             556..1479
                     /locus_tag="VIR04_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HEY9459291.1"
                     /translation="MEYLAQLIEHYGLALVFLNVLVEQLGAPLPSYPTLIVTGALLQR
                     SGSSMILLLSVAIVAALIADLAWYQAGKRYGRKVMSTLCRISLSPDSCVRQTELIYLR
                     WGARSLLVAKFIPGFASIASALAGALGTRRFGFILFDGLGAALWAGSAIYLGTLFGTT
                     IDDLLNVLENLGKWGLALIGLALLAFIARKWWQRDRFLKSIRMARISVDELHQLVQQG
                     KAPVIVDARAPLSGNEGRIPGAVNMSLEDTTLPGSATSPESEVIVYCACPNEATAARV
                     AKQLMRQGYKHVRPLSGGIDAWVAAGYSIEK"
     gene            1487..>2111
                     /locus_tag="VIR04_00675"
     CDS             1487..>2111
                     /locus_tag="VIR04_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9459292.1"
                     /translation="MHDLNDLYYYVQVIDHGGFAPAGRALGIPKSKLSRRVAMLEARL
                     GVRLIQRSTRRFSVTEIGQTYYAHCKAMLVEAEAAQEAIDVTRADPQGVVRITCPVAL
                     LHADVEMMLADCMVDNPRVTLHLEATNRRVDPVGEAIDVALRVRPPPLDDSDLVMRVL
                     ANRSQCLVASAALVALRGLPETPADLTDWPSLGSGPFQENHVWNLFGA"
BASE COUNT          410 a          626 c          620 g          455 t
ORIGIN      
        1 ccgcgcggga ggcactcttg aaatggtgca actatgggtc aatttgcccg ccaaagacaa
       61 gatggcgccg ccgggctatc agaccttgct tgaaagcgat attccgtccg tcgacctgcc
      121 cgacggcgca ggccgcgtgc gagttatcgc cggcaagtac gacggccatg aagggcccgc
      181 caaaacgttt acccctctcg acgtgtggga cctgcggctg ctgcaaggca agacagcaag
      241 cctgaccgtg cccgaaggcc gtaccttcgc cctggtcatt ttacatggca cagttttaat
      301 caatggcgaa cacatcgccc gcgaggccca gttagtcacg ctggatagcg ccggcacggc
      361 agtgtccctg gaagccaata acgatgccac gattctggca ttaagcggcg agccgatcct
      421 ggaacccatc gtcggatatg gcccgtttgt catgaattcg caagctgaaa tccagcaggc
      481 cattcaggac ttcaacagcg gccggttcgg ccagatgtct gcgtccgcct cttagacgac
      541 aggccgggcg ccgtaatgga atatcttgcg caattaatcg agcattacgg ccttgccctg
      601 gtctttctta acgtgctggt cgaacaactg ggcgccccat tgccgtccta cccaacgctg
      661 atcgtgacag gcgcgctgct gcagcgcagc ggttcttcaa tgatattgct gttgtccgtt
      721 gccattgttg ctgcactgat cgccgattta gcgtggtatc aggcggggaa gcgctatggc
      781 agaaaagtga tgtcgacact ttgccgcatt tcgctctcgc ccgattcctg cgtaaggcaa
      841 accgaactga tttatttgcg ctggggcgcc cggtcccttc tcgtcgccaa gttcattccc
      901 ggtttcgcat cgatcgccag cgcgctggcc ggcgcgcttg gaacgcggcg cttcggcttt
      961 attctcttcg atggcctggg cgccgctctt tgggcagggt cggcgattta tcttggcacg
     1021 ctatttggca cgaccattga cgatttgttg aatgtgctcg aaaacctggg taaatggggt
     1081 ttggcgctga tcggtctggc gctgctggcg tttatcgcaa ggaaatggtg gcaacgcgat
     1141 cgctttctga agtcaatacg aatggcgcgc atatccgttg acgaacttca tcaattagtg
     1201 cagcagggca aagcgcctgt tattgtcgac gcccgcgcac cgctttccgg gaatgaaggc
     1261 cgtatcccgg gcgcggttaa catgtcgctg gaagatacaa cccttcctgg ctccgccacc
     1321 tcgcccgaga gcgaagtaat cgtctattgc gcctgcccga atgaagctac cgccgcgagg
     1381 gtcgccaagc aattgatgcg acagggctat aagcatgtgc ggcctttatc gggcggcatc
     1441 gatgcatggg tggccgcggg atattccatc gaaaaataag gccgccatgc acgatttgaa
     1501 cgatctttac tactatgtcc aggtcatcga tcacggtggc tttgcaccgg ccggacgtgc
     1561 gcttggcata ccgaagtcaa aactgagccg gcgcgtggcc atgctggaag caagactggg
     1621 cgtgcgcctg attcagcgtt ccacccggcg cttttcagtc actgaaattg gtcagaccta
     1681 ctacgcgcac tgcaaggcca tgctggtgga agccgaagcc gcgcaagaag ccatcgacgt
     1741 tacgcgcgcc gatccgcaag gcgtcgtgcg tatcacttgc ccggtggcgt tgctgcacgc
     1801 agatgtcgaa atgatgcttg cggattgcat ggtcgacaat ccgcgggtca cactgcacct
     1861 ggaggccacc aatcgtcgcg tcgacccggt cggcgaagcc atcgatgtcg ccctgcgggt
     1921 gcgtccgccg ccgctcgatg acagcgatct cgtcatgcgg gtactggcca atcgcagcca
     1981 gtgcctggtc gccagcgccg cgcttgtcgc gcttcgcggg ttgcccgaaa cgcccgctga
     2041 cctcactgac tggcccagcc tgggatcagg cccttttcaa gaaaaccatg tctggaattt
     2101 attcggggcc g
//
LOCUS       DATCXU010000033         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_384694,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000033 DATCXU010000000
VERSION     DATCXU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384694"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VIR04_00680"
     CDS             <1..275
                     /locus_tag="VIR04_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9459293.1"
                     /translation="AVRLTVSDNGPGFASTVLSRVFEPYVTTKAAGTGLGLAIVKKII
                     EEHGGQIDISNRREGGARVSILLTRLAYSQSTLDEGAERYDNAAER"
     gene            286..969
                     /locus_tag="VIR04_00685"
     CDS             286..969
                     /locus_tag="VIR04_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY9459294.1"
                     /translation="MARILVVDDEVGIRELLSEILYDEGHTVELAENAAQARASRLRK
                     RPDLVLLDIWMPDTDGVSLLKEWGSQGLLDMPVIMMSGHATVDTAVEATRIGAVDFLE
                     KPITMQKLLKTIAAGLARPVVSPGLAAASRLPLDRREQAQPLTTVPVSDSVQPANELN
                     NSLLGSIALDQPLRDARDEFERIYFEYHLGRENHSMTRVSEKTGLERTHLYRKLKQLG
                     IESSRKKNI"
     gene            complement(973..1938)
                     /locus_tag="VIR04_00690"
     CDS             complement(973..1938)
                     /locus_tag="VIR04_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY9459295.1"
                     /translation="MSQPPSIPLSPAHVQLQFARRSPLDAAQFLYGEVAQRMLRRLSY
                     IRIAPANLLDAGCGAGHAIEPLRARYPDIDYTGLDACEELLEVAKSRYGAKPSLWQKL
                     RNKPTPAVHLIHADLADSGLPDQSLDLVWSNMALHWHSRPHLVLSEWRRILKKNALVM
                     FSCLGPGSLKELRQALTDAGLHTATPDFVDMHDFGDLLLERGYADPVMDQEILTLTYH
                     TPEKLLDDVHALGGNPSTARRPGLVGRSWRERLLAALEKQRNLDGTIHLSMELAYGHA
                     WRSMARGAITGETSISARSVRNARDPDAPPPRVRPRNPEDNKKIE"
     gene            2003..>2556
                     /locus_tag="VIR04_00695"
     CDS             2003..>2556
                     /locus_tag="VIR04_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY9459296.1"
                     /translation="MTYLPDWREPAPSRAAQWVLRARRHYESMLECVPASCPLCQGSA
                     RGGRLCGGCLVDVTQSMRSAAPRCAVCCLALADDGGCPDCLLRAPAFDRIVAAFDYAS
                     PGDVLIRHFKVANRFAYARMLAELLAHAVNAAMPSAPRHTILVPVPASRASINKRGFN
                     PAAELARCLSWQLPLRYRPDLLVRA"
BASE COUNT          479 a          711 c          794 g          572 t
ORIGIN      
        1 gcgcggtgcg gcttacggtg tccgataatg gtcctggttt tgcatcgact gtgctgtcgc
       61 gggtgtttga accttacgtc acgacgaagg ccgccggcac cgggttgggt ttggcgatag
      121 ttaaaaaaat tatcgaggag cacggcggcc agattgatat ttccaaccgg cgcgagggcg
      181 gggcacgcgt ctctattctc ttgacccggc ttgcgtattc acaatccact ctggacgaag
      241 gcgcggaacg gtatgataat gcagcagaaa ggtaggtgac actttatggc cagaattctg
      301 gttgtcgatg acgaagttgg catccgtgaa cttttgtcgg aaattctcta cgatgaaggt
      361 cacacggttg aactcgccga aaacgcggct caggcgcgcg catcgcgatt gcgcaagcgc
      421 cctgatttgg tcttgctcga tatctggatg ccagataccg atggggtaag tttattgaag
      481 gaatggggct cgcaagggct tttggatatg ccggtcatta tgatgagcgg gcatgccaca
      541 gtcgacaccg ccgtcgaggc cacgcgcatc ggcgccgtcg attttcttga aaaacccatc
      601 acgatgcaaa agctgctcaa gacgattgcg gccggcttgg cccggcctgt ggtttcgcct
      661 ggccttgcgg cggcatcgcg tctgcctctg gatcggcgcg agcaagctca gcccctgaca
      721 actgtgcctg tgtccgattc ggttcagccg gccaatgagc tgaataattc tttgttgggc
      781 agcattgccc ttgatcagcc gctacgcgat gcacgcgacg agttcgaacg catttatttt
      841 gaataccatt tgggccgcga aaatcacagc atgacccgtg tgtccgaaaa gacgggactt
      901 gagcgcaccc atctatatcg caaattgaag cagctcggca tcgagtcgtc gcgcaaaaag
      961 aatatttagc ctttattcta tttttttatt gtcttcggga tttcgggggc gcacgcgcgg
     1021 cggcggggcg tcgggatcgc gggcattgcg aacgctgcgc gccgaaatgc tggtttcccc
     1081 tgtaattgcc cccctggcca tcgaacgcca ggcatgccca taagcaagtt ccatactaag
     1141 atggattgtt ccatcaagat tgcgctgctt ctccagagcc gccaacaggc gctcgcgcca
     1201 gcttctgcca acgagccccg gccgccgcgc tgtgctggga ttgccgccca gcgcgtgtac
     1261 atcgtcaagc agcttttcgg gcgtgtgata cgttagtgtc aggatttcct gatccatgac
     1321 ggggtcggca taaccgcgtt caagcagcaa gtcgccgaaa tcgtgcatat cgacgaaatc
     1381 cggcgtggcg gtatgcagtc cggcgtcggt cagcgcctga cgcaattctt tcagcgaccc
     1441 cggtcccagg caagagaaca tgacaagcgc gttttttttc aggatgcggc gccattcgct
     1501 gaggacaagg tggggacgcg agtgccagtg caacgccatg ttggaccaga cgaggtccag
     1561 gctttggtcc ggcagcccgg aatcggccag atccgcatga attaagtgca ccgccggcgt
     1621 gggcttgttg cgcagttttt gccacaggct tggcttggcc ccatagcggc ttttggcgac
     1681 ctccagcaat tcttcacagg catccaggcc ggtgtagtcg atatctgggt agcgcgcgcg
     1741 cagcggctcg atggcgtgtc ctgctccgca ccccgcgtcc agcagattgg ccggggcaat
     1801 gcggatataa cttagcctgc gcagcatgcg ctgagcgacc tcgccgtaca gaaactgcgc
     1861 cgcgtccagg gggctgcgcc gggcgaattg caattgaacg tgagcggggc tcaacggtat
     1921 ggaaggtggc tgagacattt agggtgcagc gatctggtaa aagttaacga agggtggtca
     1981 gattaaggcg gaggactcca ttatgactta tctgcctgac tggcgtgaac ctgcgccaag
     2041 tcgcgccgcc caatgggtct tgcgggcccg gcgccactac gaatcaatgc tggaatgtgt
     2101 gccggcaagc tgccccttgt gtcaaggcag cgcgcgcggc ggcaggctgt gtggcggttg
     2161 tcttgtcgat gtgacgcagt ccatgcgcag cgcggcgcct cgttgcgcgg tgtgttgcct
     2221 ggcgttggcg gacgatggcg gttgccccga ctgtctgctt cgcgcccccg cgttcgaccg
     2281 cattgttgcg gcgtttgatt acgcatcgcc gggcgatgtg ctgattcgcc atttcaaggt
     2341 tgcgaatcgc tttgcgtacg cccgtatgct ggccgaattg cttgcgcatg ccgtgaacgc
     2401 cgccatgccg tcggcgccgc ggcatacaat tctggttccg gtgccggcca gccgcgcgtc
     2461 cattaataaa aggggcttta atccggccgc cgaactggcc cgttgtttat cgtggcagtt
     2521 gccgctgcga tatcggcccg atttattagt gcgcgc
//
LOCUS       DATCXU010000034         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1006868,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000034 DATCXU010000000
VERSION     DATCXU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006868"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIR04_00700"
     CDS             complement(<1..227)
                     /locus_tag="VIR04_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459297.1"
                     /translation="MMIKKSWRQTLAKSTHYALLACASATFVLASSAASAAENVRLAM
                     STQSWWPTTVAIAADRLKLFQKEGIHAEITV"
     regulatory      530..665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            801..1811
                     /locus_tag="VIR04_00705"
     CDS             801..1811
                     /locus_tag="VIR04_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY9459298.1"
                     /translation="MTVLVLTLFAMLLAILAASELFTNALEHLGERLKISEGVTGSLF
                     AAIGTALPETMVPLLALFAGTADVAANEEIGVGAILGAPLMLSTLTTCLMALAILRRR
                     RLGGRVAPECSGFVRDLDFFLFAFALATAAMFIPVDLRYVRGAFSFALIATYFVYVMH
                     TLRASDDLVRCGHGTTAANTMFLSRAGLPTNLLTIVLQLVGGLALLIFGAKGFIHGVQ
                     ELSNSLGVSVLLLSLLIIPIATELPEKVNSILWIRRGKDTLAFGNITGAMVFQGTLLP
                     ALGIMLTPWQPRVEVLTGVLITLSAAAWLRLNSRTAGLKVWALLFNGGLYATYLVVTL
                     TR"
     gene            complement(1838..2821)
                     /locus_tag="VIR04_00710"
     CDS             complement(1838..2821)
                     /locus_tag="VIR04_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459299.1"
                     /translation="MINTWIKVLTLLAASVIGIAQAQAQTTSTYPNKPIRLIVPFAPG
                     GGNDTVARTVGEQVSHILGQAVVVENRAGAGGTIGAELVARAAPDGYTLFLGGVGSLA
                     INPDMRKNLSYDPIKDFAPITLLAQAPLVLVASPALKVHSVKELIALAKSQPGKIDYA
                     SNGNGSSSHLAALMFTSMADINMLHIPYKGLSPALTGLLSGEVPTMFSSVVAILPHIQ
                     NGKLIALGVTGSTRLAALPDIPTIAQAGLTGYETSSWYGILAPAGTPHDIVLKLNKAL
                     NQALQAPDVKTRLASEGAETVGGTPEQFAAYIKQEKEQLGQVISKSGIQID"
     gene            complement(2895..>3896)
                     /locus_tag="VIR04_00715"
     CDS             complement(2895..>3896)
                     /locus_tag="VIR04_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9459300.1"
                     /translation="AAVAGYEIGPRVGICMGPEHIAQGWHSGATLGVFSSAAGAARGL
                     KLDVEHTVHALGIAGTQASGLMAAQYGAMVKRMHAGRASQSGLYGALLAEQGFTGIVN
                     VLESEYGGFCSTFSRSQDRFNLEELTAGLGQTWQTMGIALKFYSCVGSNHSTLDALRA
                     MQAQRPFTEADVEEIVVHGSQATTDHVGWKYEPQGLTSAQLNLPYCVATLLLEGDCFV
                     DQFTEDKVADPERMRAAQKVQVKHDSGITAKGSKFRHMVRVEVRFKDGTKLERTVQAG
                     RGNETDFASQADVIEKFDKLASHVLPHAQVVKIRDWMLNLEQQTDAGELARLLAKAS"
BASE COUNT          701 a         1081 c         1143 g          971 t
ORIGIN      
        1 taaacggtga tttccgcgtg gatgccctct ttttgaaaca gtttcaatcg atcggcggct
       61 atggcaacag ttgtaggcca ccatgattgc gttgacattg ccaggcggac gttttcggcc
      121 gcgctggctg cgctggatgc aaggacgaat gtagcgcttg cgcaggcaag caatgcgtag
      181 tgcgtggact tggcaagggt ttgccgccag ctttttttaa tcatcatgag tgaatctcct
      241 tttgataaaa cagggttgcc gcggtgtttg aaaagcgtaa tacatcgttg gcgctttatg
      301 gagtcaagcg catctgtaat ttttttgcct gggatcgttt cagggtataa aggcaatact
      361 cttgaaaaga gtctttttcc aaaccatcaa tcatagcagt aaatgccggc ttcgccgcgc
      421 caccaccctt ggcgggcgag gtattcgggt ggcgctgcct agacgaggcc gccaaaacgt
      481 gtcgcctgga cggggtcgcc tagacggggt cgtcattgaa tgttagactc caaatgtcat
      541 tggggagtag ccttcctccg ccccggagga gaacacgtca acatactcgg cttcggccgt
      601 ggcgcgttcg gccgatcggc ttggcgagac cattgatgta cagcttgttt cagactgaat
      661 cggctgtgca ccatggttta tcgtcatccc gaaccggagc tacttttgta cgccctattc
      721 attgcccgcc cttgtcttac tgtacccctg cgcgctgcgc gccgaattaa tcggcatggc
      781 ccatttgcgg gggtgcgccc atgactgtcc tcgtccttac cctatttgcc atgctgcttg
      841 ccattcttgc ggcgtcggaa cttttcacta atgcgctgga acatctgggc gagcgcctga
      901 aaatttccga aggcgttacg ggctctttat ttgccgccat cgggacggct ttgcctgaaa
      961 caatggttcc attactggcg ttgtttgccg gcaccgccga cgtggctgcc aacgaagaaa
     1021 ttggcgtggg tgctatcttg ggtgcacctc tgatgttgtc cacattgacc acctgtctca
     1081 tggctttggc tattttgcgc cgtcgccggc ttggtgggcg cgtggctcca gaatgcagcg
     1141 gattcgtgcg cgatcttgac ttttttctgt tcgcttttgc gctcgcgacg gccgccatgt
     1201 tcatccctgt ggatcttcgt tacgtgcgcg gggcattcag ttttgcgctg attgcgacct
     1261 attttgtata tgtcatgcat acgctgcgcg catcggacga tctggtgcgc tgcggccatg
     1321 gcaccacggc cgcaaacacg atgtttttat cgagggccgg tctgcccacc aacttgctga
     1381 ccattgtgct gcagctcgtt ggtggtctcg cattgctgat tttcggcgcc aagggtttca
     1441 ttcacggcgt ccaggaacta tcgaactccc tgggcgtttc ggtgctgctg ctttcgttgc
     1501 tcataattcc gatcgccacc gagcttcccg aaaaagtgaa tagcattttg tggattcggc
     1561 gcggcaagga cacgttggcg tttgggaata ttaccggcgc catggtgttt cagggtacgc
     1621 ttttgccggc gctgggaatt atgttgacgc cgtggcagcc ccgggtagag gttttgaccg
     1681 gggtgttgat cacgttgtcg gcggccgcct ggctgcggtt gaattcccgt accgcaggtt
     1741 tgaaagtgtg ggcgctgcta ttcaatggcg gactttacgc gacgtatttg gtcgtcacac
     1801 taacgcggtg aatgtgccag cctgtcggtt gatcatttca atctatctgg attcctgatt
     1861 tggagattac ctggcccaat tgctcttttt cctgcttgat ataggcagca aactgttcgg
     1921 gagtgcctcc tactgtttcg gcgccttcgc tggccagccg cgttttgacg tcgggggcct
     1981 gcaacgcctg gtttagcgcc ttgttaagct tgagaacgat atcgtgcggc gtgccggccg
     2041 gcgccagtat gccgtaccat gaacttgttt cgtagcccgt caagccggcc tgcgcgatgg
     2101 tgggaatatc aggcaacgcg gccagtcgtg ttgaaccggt gactcccagc gcgataagtt
     2161 tgccgttttg aatatgcggc aggatcgcca ccacactgct aaacattgtg ggcacctctc
     2221 cgcttaatag gcccgtaagc gcggggctta gccccttgta agggatatgc agcatattga
     2281 tgtcggccat cgaggtaaac atgagcgccg caagatgcga ggaagagcca ttgccgttag
     2341 acgcataatc gatttttccc ggttgcgatt tggccagcgc gatgagttct ttaacggaat
     2401 ggacttttag cgccgggctt gcgaccagga ccagcggcgc ctgagccagc agcgtgatag
     2461 gggcaaagtc tttgatgggg tcgtaagaca ggtttttcct catgtcggga ttgatggcca
     2521 gactaccgac tccgcccaga aacaatgtgt agccatcggg ggcggctctt gccaccagtt
     2581 ccgcgccgat ggtgccgccg gcgcccgcgc ggttttcaac gactacggcc tgcccgagaa
     2641 tatgcgatac ctgttccccg acggtgcgcg ccacggtatc gtttccgccg cctggcgcga
     2701 agggcacgat caggcggata ggcttgtttg gatacgttga ggtggtctgc gcctgcgcct
     2761 gtgcgatgcc gatgacgctg gcggccaata gtgtaagaac ctttatccag gtattgatca
     2821 ttgcgcaggc cttgaaatag ttcgacttgt tattgatgtc atggcgtctt gctacggcgt
     2881 cggggttaaa tatatcacga cgctttagcc agcaagcgcg ccagctcgcc tgcgtcggtc
     2941 tgttgttcca ggttcagcat ccagtcgcga atcttcacga cttgcgcatg cggcaagaca
     3001 tgcgacgcca gcttgtcgaa tttctcgatg acatcggcct ggctggcaaa atctgtttcg
     3061 ttgccgcgtc ccgcttgtac ggtgcgctcc agcttcgttc cgtccttgaa ccgcacttca
     3121 acccgaacca tgtgacgaaa cttcgagcct tttgcggtga tgccgctgtc gtgtttgacc
     3181 tggacttttt gggccgcgcg catgcgttcg gggtcggcaa ctttgtcttc ggtgaattgg
     3241 tcaacaaagc agtcgccctc cagcaacaag gttgccacgc aatagggcag attgagttgg
     3301 gccgaggtca ggccctgcgg ttcgtatttc cagcccacat ggtccgtcgt cgcctgcgag
     3361 ccatgcacga cgatttcttc cacgtcggcc tcggtgaacg ggcgttgcgc ctgcatggcg
     3421 cgcagcgcat ccagcgtgct gtggttgctg cctacgcaag agtagaactt caaggcgatg
     3481 cccatagtct gccaggtctg ccccagaccg gcagtgagtt cttccagatt gaaccggtcc
     3541 tgcgagcgcg aaaaggtgct gcaaaagccg ccgtattcgc tctcgagcac attgacgatg
     3601 ccggtgaagc cctgctcggc caaaagcgcg ccgtatagcc cgctttgcga ggcgcggccg
     3661 gcatgcatgc gtttgaccat tgcaccatat tgcgccgcca tcagtccgct ggcctgtgtg
     3721 ccggcaatgc ccagagcatg cacggtgtgt tccacatcca acttcagacc gcgtgcggcg
     3781 ccggcggccg acgagaacac gcccagcgtc gcgcccgagt gccagccctg agcgatatgt
     3841 tcgggcccca tgcaaatacc gacgcggggg cctatttcgt aacccgcaac ggcggc
//
LOCUS       DATCXU010000035         4632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_384228,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000035 DATCXU010000000
VERSION     DATCXU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384228"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1025
                     /locus_tag="VIR04_00720"
     CDS             138..1025
                     /locus_tag="VIR04_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459301.1"
                     /translation="MTALFGIPIQALLGQLLLGLVNGSFYAVLSLGLAVIFGLLNVIN
                     FAHGALYMLGAFVAWMGLQYLGLNYWIMLIVAPIVVGLFGILIERLLLRHLYKLDSLY
                     GLLFTFGLTLVIEGVFRTIYGVSGQPYPTPELLQGGVNLGFMYLPIYRGWVVGASIVA
                     CLATWLMIEKTRLGAVLRAGIENPKLVEAFGVNVPLVVTLTFGFGIALAGFAGVLAAP
                     VLPVSPLMGSNLIIVVFAVVVIGGMGSILGSIVTGLGLGVIEGLTKVFWPEASNTVVF
                     IIMVIVLLVRPAGLFGKEK"
     gene            1022..2032
                     /locus_tag="VIR04_00725"
     CDS             1022..2032
                     /locus_tag="VIR04_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459302.1"
                     /translation="MKRQLIAYLLAAIVIALLPALGAYPIFVMKVLCYALFACAFNLL
                     LGYTGLLSFGHAAFLGGAAYATGHALAVWGFSTATGLLFGTAVAAIMGLIMGALAIRR
                     SGIYFAMITLAMAQMVFFYFLQAPFTHGEDGLQGIPRGTLFGLDLSEDINLYYVVLVI
                     FIFGFAVCWRAVNSPFGQVLKAIRENEPRAISLGYNVDSFKLLAFVLSAALSGLAGAT
                     KAVVFVSATLTDATWQLSGLVILMTLIGGMGTFTGPVLGAFIIVLLENKVGEFGHFMA
                     RLTGFQWFQAMGESVTIVIGLIFIICVMSFRRGIVGELGAYIKSRKAKSHEVNSTIPG
                     TI"
     gene            2120..2515
                     /gene="msrB"
                     /locus_tag="VIR04_00730"
     CDS             2120..2515
                     /gene="msrB"
                     /locus_tag="VIR04_00730"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY9459303.1"
                     /translation="MEKIRKTDAQWRQQLSPEEYAVARQKSTERPFTGRYWNNSERGI
                     YRCVGCGTPLFASDTKFDAGCGWPSYFEPLDPDLVREERDTSLGMVRTEVLCNVCDSH
                     LGHVFPDGPPPTGLRYCLNSVSLKFEPTA"
     gene            2512..3102
                     /locus_tag="VIR04_00735"
     CDS             2512..3102
                     /locus_tag="VIR04_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY9459304.1"
                     /translation="MKKFLFDLFPLILFFLAFKFSDIFTATAVAIVAGIVQFIWLKAT
                     GRAIEATHWINLTVIIVFGGATLLFHNDAFIKWKPTVLYWLFASIVLVGKFVFKRNLI
                     QKVMSAQIDMPESVWNKLSLSWAGFFILSGALNLYVAFSGYFTESQWVNFKVFGLMAL
                     LLLFVIAQSIWLSRYMKDNIGPSPAVSGSREPKEPS"
     gene            3102..3359
                     /locus_tag="VIR04_00740"
     CDS             3102..3359
                     /locus_tag="VIR04_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY9459305.1"
                     /translation="MSDRSDLIKERLSVLAPVQFEITDESHLHAGHEGSKNGASHFRI
                     VISSSRFNGLPVLERHRLVYELLHDLIPFPIHALAIVAKPV"
     gene            3374..4150
                     /locus_tag="VIR04_00745"
     CDS             3374..4150
                     /locus_tag="VIR04_00745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY9459306.1"
                     /translation="MKRFVVFAAACAIALPVYAQTVATVNGQTITQASLDQFVSLLVG
                     QGAKDSPELRAQVKQEMINRAVAVQAAKKAGLDKQESVKQEIELARESILVRALMADY
                     LKKNPVSDAKIQAEYDTIQKEQANKQEYKLRHILLKDEKTAKDLTASIKSKKITFAAA
                     AKKDSLDLGSGKNGGELGWAPTTNYVPEFAQAVEHMKKGQMTDKPVQTQFGWHIIEVE
                     DSRPLKFPDLQEVKPKLEEMLRQQELAAYQESLIKAATIK"
     gene            complement(4292..>4632)
                     /locus_tag="VIR04_00750"
     CDS             complement(4292..>4632)
                     /locus_tag="VIR04_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEY9459307.1"
                     /translation="SIVHFFAEPHNREVVHALAAAGVVAKLDPRPVGQHQRLRGKTLV
                     LTGALPHWSRDEATQHIMAVGGKVSGSVSKKTDYVVAGEDAGSKLARARELGVAILDE
                     AGLIALLRGV"
BASE COUNT         1005 a         1293 c         1230 g         1104 t
ORIGIN      
        1 atccggctcg gcggccgaat ccacctgcgc tttcgctaaa aagtaactca gagtgtagaa
       61 ggcctgcttc cccgccatgg ggggcaggtc gtttgttact tgaaacattc acgtttcgcc
      121 acggactttt gacacctatg actgcactat tcggcatacc tatccaagct ctcttgggtc
      181 agttattgct aggcctggta aatggctcgt tttacgccgt actatcgctg ggtcttgccg
      241 taatctttgg tttgcttaat gtcattaatt ttgcacacgg cgctctttac atgctgggcg
      301 cattcgtggc ctggatgggc cttcaatatc tgggcctgaa ttactggatc atgctgatcg
      361 tcgcgccgat cgttgtcggc ttgttcggca tactcatcga acgcttgctt ttgcggcatc
      421 tgtacaagct cgacagcctg tacggtctgc tattcacctt cgggctgacc ctcgtcatcg
      481 agggcgtgtt tcgcacaata tacggcgtat cggggcaacc ttacccaaca ccggagcttc
      541 tacaaggcgg agtcaacctg ggcttcatgt acttgcccat ttaccgcggc tgggtcgtcg
      601 gcgcctccat cgtagcgtgc ctggcgacgt ggctcatgat cgaaaaaaca cgcctggggg
      661 cagtgctgcg tgccggcatc gaaaacccga aactggtcga agctttcggc gtgaacgtgc
      721 cgctggtcgt tacgcttaca ttcggattcg gcatcgccct ggccggattc gccggcgtgc
      781 tggccgcgcc cgttctgcca gtttcaccgc taatgggttc gaacctgatt atcgtggtct
      841 tcgcggtggt tgtgatcggc ggcatgggat caatcctggg atcgatcgtg accggtctgg
      901 gcctgggcgt catcgaaggg ctcaccaaag tattctggcc ggaagcctcc aataccgtcg
      961 tgttcattat catggtcatt gtcttgttgg tgcgcccggc cggcctgttt gggaaagaaa
     1021 aatgaagcgt caattaatag cttacctgct ggcggcgatt gtcatcgcat tacttccggc
     1081 actgggcgct tacccgatct tcgtcatgaa ggtcctgtgc tacgcgcttt ttgcctgcgc
     1141 cttcaacctg ttgctgggct acacaggctt attgtcattc gggcatgccg cgtttctggg
     1201 cggagccgcc tacgccaccg gacacgcgct ggctgtgtgg gggttttcaa ccgcaacggg
     1261 gctattgttc ggcaccgcgg tggctgccat catggggcta atcatgggcg cgctggcaat
     1321 ccggcgcagt ggcatttact ttgccatgat cacgctggcc atggcgcaga tggtgttctt
     1381 ttacttcctc caagcaccct tcacacacgg cgaagatggc ctgcaaggca ttccgcgcgg
     1441 cacgctcttc ggcctggatc ttagcgagga catcaatctt tattacgtcg tcctggtcat
     1501 tttcatattc ggctttgccg tgtgctggcg cgccgtaaac tcgccgttcg gccaggtgct
     1561 gaaggccata cgcgaaaacg aaccgcgcgc catttccctg ggctataacg tagacagctt
     1621 caagctcctg gcttttgtcc tgtcggcggc gctatcgggc ctggcaggcg caaccaaggc
     1681 agtcgtattt gtttcagcca cgctcaccga cgcgacgtgg cagttgtcgg gcctggttat
     1741 ccttatgacc ctgatcggcg gcatgggcac cttcacgggc cccgtgctgg gcgctttcat
     1801 tattgtcctg ctcgaaaata aagtgggtga atttggccat tttatggcca gactcaccgg
     1861 attccaatgg tttcaggcca tgggcgagtc agtcaccatc gttatcggcc tgattttcat
     1921 tatttgcgtg atgtcattcc ggcgcggcat cgtgggcgag cttggcgcct acatcaaatc
     1981 ccgaaaagcc aaatcgcacg aggtgaactc caccatcccc gggacgattt gatatccagt
     2041 ccgcaagtcc tgttttttgt actgcaataa tgcattaaca tggcaagttg tgcacaccag
     2101 tacataagca ggcaccgaaa tggaaaagat acggaaaacc gacgcccaat ggcgtcaaca
     2161 gctcagtccc gaagaatatg cggtcgcccg ccaaaaaagc accgagcggc cgttcaccgg
     2221 ccgctactgg aacaattccg aacgcggcat ctaccgctgc gtgggttgcg gcacgccctt
     2281 gtttgcctct gacaccaaat tcgacgcggg ctgcggctgg cccagctact ttgagcccct
     2341 ggatcctgat ctcgtgcgcg aagaacgcga cacaagcctg ggcatggtgc gcaccgaagt
     2401 gctgtgcaac gtatgcgact cccacttggg tcatgtcttc ccggacggcc cgcctccgac
     2461 cgggttgcgt tactgcctga actccgtctc actgaagttt gaaccaaccg catgaagaaa
     2521 tttcttttcg atctgtttcc gctgatcctt ttttttctgg cgttcaagtt ttccgatatc
     2581 ttcacggcga cggccgttgc catcgtcgcg gggatagttc agttcatctg gctgaaggcg
     2641 accggccgtg ctatcgaagc cacccactgg atcaacctta cggtcatcat cgtattcggc
     2701 ggagccacgc tgctctttca caacgatgca tttattaaat ggaagcccac ggtcttgtac
     2761 tggctttttg ccagcatcgt gctcgtagga aaattcgttt tcaaacgcaa cctaattcaa
     2821 aaagtgatga gcgctcaaat cgacatgcca gagagcgtct ggaacaagct tagcttaagc
     2881 tgggctggct tcttcatctt gtcaggagcc cttaatttat atgtggcgtt ttccggctac
     2941 ttcaccgaat cgcaatgggt aaacttcaaa gtattcggtc ttatggcgtt gctgttgctc
     3001 tttgtcattg cgcaatcgat ctggcttagc cgctacatga aagacaatat tggaccatca
     3061 ccggctgttt caggctcaag ggaaccaaag gagccttcat gatgtctgac cgttcggatc
     3121 tgataaagga gcgcttaagc gttctagcac cggtgcaatt tgaaatcacg gacgaatctc
     3181 acttgcacgc agggcacgaa ggcagtaaaa acggcgcaag ccattttcgc attgtcatct
     3241 cgtcatccag atttaacggg ttgccggtcc tggagcgcca tcgcctcgtc tatgaacttt
     3301 tacatgattt aattccattc cccattcacg cactggccat cgttgccaaa cctgtttaaa
     3361 tcaaaggaaa cacatgaaac gtttcgtcgt atttgccgcc gcctgcgcca ttgctctgcc
     3421 agtctatgcg caaactgtag ccaccgttaa cggacaaacc ataacccagg ccagtctgga
     3481 tcaattcgtt tcgctgttgg tgggccaagg cgcgaaggat tcaccggagc tgcgtgccca
     3541 ggtcaagcaa gaaatgatca accgcgccgt ggcagtgcaa gccgccaaga aggcgggcct
     3601 cgataaacag gaaagcgtca agcaagagat cgaactcgcc cgtgaaagta tcctggtgcg
     3661 cgctctgatg gccgattacc ttaagaaaaa ccctgtgtcc gacgccaaaa tccaggctga
     3721 atacgacaca attcagaaag aacaggcgaa caagcaagaa tacaagttgc gccatatctt
     3781 gctaaaggat gaaaaaacgg ccaaggatct gaccgcatcc atcaagagca aaaaaattac
     3841 tttcgccgct gccgcgaaga aagattcgct tgatcttggc agcgggaaaa acggtggaga
     3901 gttgggttgg gcgccaacca caaactatgt acctgaattc gcccaggctg tcgaacacat
     3961 gaaaaaggga caaatgacgg ataagccggt tcaaacccag tttggctggc acattatcga
     4021 agttgaggac tcccgcccgc ttaagttccc cgacttgcaa gaagtcaaac ccaagctgga
     4081 agaaatgctg cgccagcaag aattggccgc ctatcaagaa agtttgatca aagccgcgac
     4141 gattaaatag ctagcgtggt ggcgcatttg ggtcagattt aaaaaggctg aaccaaagga
     4201 atagcagcac gacgcagcga ggtctgtgcc accccgctgc gtctggtgaa ttttggagct
     4261 gcgcatgcca ggacagcgcc tcagcgcaaa tctacacgcc cctcagcaat gcaatcagcc
     4321 cagcctcgtc aagaatggca acgcctaatt ctcgtgctcg cgcaagcttg ctgccggcgt
     4381 cttcgccggc gaccacgtag tccgttttct tcgacacgga gccgctgact ttgccgccta
     4441 ccgccatgat gtgctgagtg gcttcgtcgc gcgaccaatg aggcaaagcg cctgtcagca
     4501 ccagtgtttt accgcgcaag cgctggtgtt ggcccaccgg cctggggtct aatttggcga
     4561 ccacgccggc tgcggccaac gcatggacga cttcgcgatt atgcggttcg gcaaaaaaat
     4621 gcacgatgga tt
//
LOCUS       DATCXU010000036         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_221333,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000036 DATCXU010000000
VERSION     DATCXU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221333"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VIR04_00755"
     CDS             <1..995
                     /locus_tag="VIR04_00755"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEY9459308.1"
                     /translation="FVREHIQPDRHVHLVLENDDNTASLMKEGFNAQWNDDAHHVLHH
                     LLTGETQGYYGAYAQQPTENLARFLAEGFVYQGQPSLWRAGKPRGEPSADLSPTCFIF
                     FLQNHDQIGNRAFGERLVSLCGSASPALHAAVTLQLLTPHIPLLFMGEEVGATTPFQY
                     FTGYDDPALARAVREGRRNEFAAFLHFIDPREQVRIPDPNAMTTWQRSLPACWPADIQ
                     GRDWRDWYRRLLALRAQFITPYLDQARSAGVDILASRAVHAKWILGNGSLLSIYCNLA
                     KEPCICPEPAGESADFTLFASKKDADKAIHHGCLPAVCTVAVLLPAPNCEVPHG"
     gene            988..3177
                     /gene="malQ"
                     /locus_tag="VIR04_00760"
     CDS             988..3177
                     /gene="malQ"
                     /locus_tag="VIR04_00760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY9459309.1"
                     /translation="MASVPVTDRALAKLAELAGLVEHWQDVYGRKHQAPRPTLQFLLN
                     TLDLPSGSSSQIMESTRKLEHESRCDSDRLILADTDTRPTFRHNGSLSYTLTLENGEQ
                     RSGTATRIGYGMASIAPVTVAGYHLLTIGDSPVALAVAPRHCPSMPDLTGKARPRLWG
                     IAAQLYSLRRTMRCPASTLVAGSKTNTLSSTLAGLHGAGDYSALAQLVHQAAAQGADA
                     VAISPVHAMFSADPSRYSPYAPSSRLFLNTAYIDPAIVFGNDAIESALPGLACGMSVA
                     SRDEAEIDWPRVVRTRMQLFRHLYEQFLAQAPHRLARAFQTFRQKGGEALESHSRYEA
                     LHTHYLPALGADSGWREWPAELHDPAGPAVRAYAAAHDKEVGFHAFLQWMADEGMRFA
                     QESACHAGMSIGLITDLAIGTDPRGSHAWSRQGDILNGVSVGAAPDLYHAKGQNWGLT
                     AFSPWALRRNAYASFIETVRAALTHAGGLRVDHILGLARMWLVPQGAEASEGVYLRYP
                     LEDMLRLLVLEAWRHSALVIGENLGTVPAGFNQRLAKKGVFGTAVLWFERETAMTDKE
                     HVAAFSLPKNWPPKAVATSTTHDLPTIAGWWSGRDLQWLKQMEQITEPEFNDLTQERG
                     RDKTALWRALYQAGCVTVDVLSPPFCAPREAILSFVASAPTPLVVIPIEDLLGLVEQP
                     NLPGADGPVPPFHPNWQRRLPDSVENLFRDPGLRGCTAAVARSREAA"
     gene            3174..>4495
                     /gene="treY"
                     /locus_tag="VIR04_00765"
     CDS             3174..>4495
                     /gene="treY"
                     /locus_tag="VIR04_00765"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY9459310.1"
                     /translation="MSTPRATARLQLHAGFTFDDARAQIPYYAKLGISHFYLSPISCA
                     RPGSTHGYDVVDHRIVNPELGGESAFARLARDLQDHAMGIILDIVPNHMATHSDNHWW
                     WDVLRHGQGSAYADWFDIDWNSPDHELRGKVLAPFLAESYGQSLIDGRIKLIFDDASQ
                     TFQIQACDARYPLAPGTLDKDAKSISRTLARYDAADAVGRERLHQLLQQQHYVLSWWR
                     CAADRINWRRFFEVTDLIGLCVERRQVFEAVHALPLRLFAEGLIDGVRVDHVDGLAQP
                     LAYCRQLRTELEARSARREPASRNDEPWLIVEKILASGETLDQRWAADGTSGYDFMDQ
                     ASAVLHDPAGESDLTARWIDVCGDPSSLRTIVQDARRLMLHGHFAAERRALLNAFTLL
                     AQSDMATRDWTGQAIARVLDALLILFPVYRTYIEGNAADASDAELLRRV"
BASE COUNT          899 a         1290 c         1327 g          979 t
ORIGIN      
        1 aatttgtgcg tgaacatatc cagccagaca ggcatgttca cctggttctc gaaaatgacg
       61 acaataccgc ctcgttaatg aaagagggtt ttaatgcgca atggaatgat gacgcgcacc
      121 acgtattgca tcatttgctg acaggcgaaa ctcaaggtta ttacggcgcg tatgcgcaac
      181 agcccaccga gaatctggcg cgttttctgg ccgagggttt tgtatatcag ggacagccaa
      241 gtttatggcg cgcgggcaag ccacgcggcg aacccagcgc agacctgtct cctacttgct
      301 ttattttttt cttgcagaat catgatcaga tcggaaaccg cgcattcggc gaacggcttg
      361 tttccttgtg tgggtcggcc tcgcccgctt tgcatgccgc cgttacattg cagttgttga
      421 cgccgcatat cccattatta ttcatggggg aggaggtggg agcaacaacc cctttccagt
      481 atttcaccgg ctatgatgat ccggctcttg cccgtgcagt gcgtgaaggc agacgcaacg
      541 aatttgctgc tttccttcac ttcatcgatc cgcgtgaaca ggtgcgaatc cccgatccga
      601 acgccatgac aacgtggcaa cgctcgcttc cggcttgttg gccggccgat attcaaggcc
      661 gtgactggcg cgattggtac cgccgcctgt tggccctgcg agcacaattt attacgccgt
      721 atctggatca ggcgcgcagc gccggcgtag atattctcgc ttcgcgcgcc gtgcacgcca
      781 aatggatatt gggcaatggg agccttctga gcatttactg caatttggca aaagagccgt
      841 gcatttgtcc ggagccggcc ggcgagagcg cggatttcac cttgtttgcg agtaaaaagg
      901 acgccgacaa agcaatacac cacggatgtc ttcccgcggt atgcactgtg gcggtattgc
      961 ttccggcgcc aaactgcgaa gtgccgcatg gctagtgttc cggtcactga tcgcgcattg
     1021 gcgaagctgg ccgaactggc cggactggtt gaacactggc aagacgtgta cggccgcaaa
     1081 catcaggccc cgcgtccaac gttgcagttc ctgctcaaca cgcttgattt gccatccggt
     1141 tcctcgtctc aaatcatgga aagcactcgc aagctagaac atgaatcgcg ctgcgacagt
     1201 gatcgcttga tcctcgccga cactgacaca cgtcccacgt tcagacacaa cggatcccta
     1261 agctatacgt tgacccttga gaatggcgaa caacgcagcg gcacggcgac aagaatcggt
     1321 tacggtatgg cgagcattgc gccggttacc gttgccgggt accacttgct gactatcggc
     1381 gacagtccag tagcgttggc agttgcgcct cggcactgtc cgtctatgcc cgacctgaca
     1441 ggcaaggcga gaccacgcct gtggggaatc gccgcgcagt tatacagctt gcgtcgaacc
     1501 atgcggtgcc cagcctccac acttgttgcg ggaagcaaga caaacacatt atcgtcaacc
     1561 ctcgcaggcc tgcacggcgc cggcgactat tcagccctgg cgcaactcgt ccatcaggca
     1621 gcggctcaag gagccgatgc ggtggcaatc agcccggtcc acgcaatgtt tagcgccgac
     1681 cccagccgtt acagcccgta cgcgccatca agccgcctct ttctgaacac ggcttatatt
     1741 gatccggcta ttgttttcgg caacgatgcc atcgaatccg ccttgcccgg tttggcctgc
     1801 ggcatgtcgg tggcaagtcg ggacgaagcc gaaattgatt ggccccgcgt agtgcggacg
     1861 cgcatgcaat tgtttcgcca tttgtacgaa caatttcttg cgcaggctcc gcatcgtctc
     1921 gcacgcgcgt tccagacctt tcgccaaaag ggcggcgaag cgctcgaaag ccatagtcgc
     1981 tatgaagcat tgcataccca ttacctgccc gcgctgggcg ccgacagcgg ctggcgcgag
     2041 tggccggccg aattgcacga tcccgccggc ccggccgtgc gtgcctacgc cgcggcacac
     2101 gacaaggaag tgggcttcca cgcatttttg cagtggatgg ctgacgaagg catgcgattc
     2161 gcccaagaga gcgcttgcca tgcgggcatg tccataggcc tgattacaga cttggcgatt
     2221 gggaccgacc cgcgcggcag ccatgcctgg agtcgacagg gcgacatcct gaacggggtt
     2281 tcggttggcg ctgcgcccga tttgtatcat gcgaaagggc agaactgggg gctgaccgca
     2341 ttctcaccgt gggcgctgcg tcgcaatgcc tatgcttcgt ttattgaaac ggtacgcgcc
     2401 gcgttgacgc atgcgggcgg attgcgcgtc gaccatatat tggggttggc gcgcatgtgg
     2461 ctggttcccc aaggcgctga ggcgtctgag ggtgtttact tgcgttaccc gctggaagat
     2521 atgttgcgat tgctggtatt ggaagcgtgg cgccacagcg cgcttgtcat aggggaaaat
     2581 ctgggcacgg tgccagccgg attcaatcag cgcctggcca aaaaaggcgt attcggcacc
     2641 gccgttctat ggtttgaacg cgagaccgcc atgaccgata aggagcacgt tgcagccttt
     2701 tcccttccaa aaaattggcc gcccaaggcg gtcgcgactt ccactacgca tgaccttcct
     2761 actatcgcgg gctggtggtc cgggcgcgat ttgcaatggc ttaaacaaat ggagcagatc
     2821 acagagcctg aattcaacga cctgacgcag gagcgcgggc gcgacaagac cgcactttgg
     2881 cgcgcgctgt atcaggctgg ttgtgtaacg gtcgatgttc tttcgccacc tttttgcgcg
     2941 ccccgcgagg ccattttgtc atttgttgcg agcgcgccga ctcctctggt ggtgatcccg
     3001 atcgaagacc ttctgggtct ggttgaacag cccaatttgc ccggcgccga cgggccagtg
     3061 ccgcctttcc acccaaactg gcagcggcgt ctgccggact ccgtcgaaaa tttgttcaga
     3121 gaccccggcc tgcgcggttg tacggcagcc gtcgcccgtt cgcgcgaggc ggcatgagta
     3181 cgccgcgcgc cactgcacgg ctgcagttgc atgccggttt caccttcgat gatgcgcgcg
     3241 ctcaaatacc gtattacgcc aagcttggga tcagtcattt ttacttatcg cccatttcgt
     3301 gcgcaagacc aggttcgacg cacggttatg acgtggttga tcatcgaatc gtcaacccgg
     3361 agctgggtgg cgagagcgcc ttcgccaggt tggcgcgcga tttgcaagac catgcgatgg
     3421 gcataatcct ggatatcgtg cccaaccata tggcgacgca ttccgacaat cattggtggt
     3481 gggatgtttt gcgccatggg cagggaagtg cctacgcgga ctggttcgat attgactgga
     3541 attcgcccga tcatgaattg cgcggcaagg tcctggctcc atttttggcc gaaagctatg
     3601 gtcaatcgct tatcgacggg cggataaagc tgattttcga cgatgcgtct caaacgttcc
     3661 agattcaggc ttgcgacgcc cgctacccac ttgcgccggg gacgctggac aaggacgcca
     3721 aatcaatatc ccgaacgttg gcgcggtacg acgcggccga tgcagtaggg cgcgagcgct
     3781 tgcaccagtt gttgcaacag cagcactacg tgctgtcctg gtggcgttgc gcggctgacc
     3841 gtatcaattg gcgccgtttc ttcgaagtta cggatcttat cggcctttgc gtcgagcgac
     3901 ggcaggtgtt cgaggcggtg catgcattgc cgttgcggct attcgctgaa ggtttgatcg
     3961 atggtgtgcg tgttgatcat gttgacggcc tggcgcaacc actggcgtac tgccgccaat
     4021 tgcgaaccga actggaagcc cgttcggccc gccgagagcc tgcgtcacgc aacgatgaac
     4081 cgtggctgat tgttgaaaaa attctggcca gtggcgagac actggatcag agatgggcgg
     4141 cggatggcac aagcggctat gattttatgg accaggcaag cgccgtgctg cacgaccccg
     4201 ccggcgagtc cgatttgacc gctcgatgga ttgatgtctg tggcgaccca agttcgttgc
     4261 gaacaatcgt tcaggatgcc agacggctaa tgctgcatgg gcattttgcg gccgaacgcc
     4321 gggcattgct gaatgccttc acacttctgg cgcaatctga catggcaacg cgcgactgga
     4381 cagggcaagc gatcgcacgg gtgctggacg cactcctgat tctatttcct gtgtaccgaa
     4441 cctacattga aggtaacgcg gccgatgcca gcgacgccga acttctaaga cgcgt
//
LOCUS       DATCXU010000037         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_462929,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000037 DATCXU010000000
VERSION     DATCXU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462929"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VIR04_00770"
     CDS             <1..202
                     /locus_tag="VIR04_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY9459311.1"
                     /translation="YLGGGIVPKLGDYVLQSPFRSRFESKGRLRSYLQPVPAFVITDP
                     YAGLLGAAAYLDANADDKEAAG"
     gene            complement(242..1591)
                     /locus_tag="VIR04_00775"
     CDS             complement(242..1591)
                     /locus_tag="VIR04_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459312.1"
                     /translation="MTLHQDHDGHVAPLRKSSPKEYITAGFASMAGTTIEWYDFFLYG
                     TAAALIFNKIFFPQFDPLMGTLAAFATYSVGFFARPFGGIIFGHFGDRIGRKSMLLTT
                     LVLMGVPTILIGLIPSYESIGYWAAVLLVSMRFLQGIAVGGEWGGAVLVAVEHAPDGK
                     KGLFGSLPQMGVGAGLILSSFAMAAVARLPEADMLSWGWRIPFLASAVLLLIGWFIRI
                     KVAESPDFEAMRQSGKKVAVPLVTVLKNHPKEVLLVIGARLGEVTWFYTVVTFALAYA
                     TGTLGIQKSVMLDAIIWGAFLSLFTMPLCGTLGDRIGHKWVFMIGAVGIGVFGMFFFG
                     MLETRESGWITLAMILAISVVYGCLYGPEGSLFSNQFPPEVRYSGISLAVQISGAVGG
                     GLAPLVATWLLAVGDGSPRYVVGYLITLGLIAFFSAWRMHSVSSSRLAAKPLVPRGA"
     gene            complement(1631..>2314)
                     /locus_tag="VIR04_00780"
     CDS             complement(1631..>2314)
                     /locus_tag="VIR04_00780"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY9459313.1"
                     /translation="LAYTCRILAATGQEAGLAGQISARSERPGAYWTLRFGLGFDEAT
                     ADDFIEVGPDLETLTGEGMANPATRFHLWVYKARPDICSMVHAHSPWVSALVAARQTL
                     VIAQMDMAPFHNDCAFLADWPGVPIADQEGVLISAALGSKRSIILAHHGYLTTGGTVE
                     EATYLSVYLERAARMQTRARVYGELVPVPAELAQEAHDYLLQPSIVKGTFDYWCRQID
                     TAAGGAVKV"
BASE COUNT          588 a          703 c          589 g          434 t
ORIGIN      
        1 ctacctgggc ggaggcatcg ttccaaaatt aggagactat gtcttgcagt cgccatttcg
       61 cagccgtttc gaaagcaagg gccggcttcg cagctacctg cagccggtac cggcctttgt
      121 gattaccgac ccctatgccg gcctgctagg cgcggcggcg tatctcgatg ctaatgcgga
      181 tgataaagaa gcggccgggt agaaaccggc cgctcatcat gcctatataa attttggcgt
      241 tttacgcgcc gcgcggcacc aatggctttg ctgccaaacg ggatgacgaa acggaatgca
      301 tgcgccaggc gctaaaaaac gcaatcaagc ctaacgtaat caggtacccg acgacgtaac
      361 gtggactgcc atcgcccacg gccagcagcc aggttgccac cagcggggcc aggccgcccc
      421 caaccgctcc cgaaatttga acggcaagcg aaatgccgct atagcgcacc tccggcggaa
      481 actggttgga aaacaggctg ccttcaggcc cataaaggca gccatacacc acactgatcg
      541 ctaatatcat tgcaagcgtg atccagcctg actcacgcgt ttccagcatg ccaaaaaaga
      601 acatgccaaa aacgcctatg ccgaccgcgc caatcatgaa tacccatttg tgaccgattc
      661 gatcacccag cgtgccgcac aaaggcatcg taaaaagcga cagaaaggcc ccccatatta
      721 tcgcgtcgag catgaccgat ttctggatgc ccaacgtacc cgtcgcgtac gcaagggcaa
      781 aagtgacgac agtgtaaaac caggttacct cgcccagcct cgcgccaatg accagcaata
      841 cttctttcgg gtgattcttc aacacagtca ccagcggcac cgccaccttt ttaccggact
      901 gccgcattgc ctcgaagtcg ggcgactcgg caaccttgat acgaatgaac cagccaatca
      961 gcaacaatac tgcgctggcc agaaatggaa tgcgccagcc ccaggacagc atgtccgctt
     1021 caggcagtcg ggccacggcc gccatggcaa acgaagacag aatcagcccg gcaccgacgc
     1081 ccatttgagg caggctgcca aacaggcctt tcttgccatc gggcgcatgc tcgaccgcga
     1141 ccaataccgc gccgccccat tcaccaccga cggcaatgcc ttgcaaaaag cgcatggaga
     1201 ccagtaaaac agccgcccaa tagccgatgg attcgtatga tggaatcaaa ccaatcaaaa
     1261 tggtcggcac tcccattagt accagagtgg tcaacaacat ggacttgcgg ccaatccgat
     1321 cgccaaagtg gccgaaaata atccccccaa acggtcgcgc aaaaaatccc accgaataag
     1381 tcgcaaaagc agccaatgtg cccattaatg gatcaaactg cggaaagaaa atcttgttga
     1441 aaatcagggc ggcagccgtt ccgtacagaa aaaaatcgta ccactcgatt gtggtgccgg
     1501 ccatactggc gaagcccgct gtaatgtact ctttgggtga actcttgcgc agcggcgcaa
     1561 cgtgcccatc atggtcctga tgcaaagtca tgtgtcttcc tccttggttt ttttaagaat
     1621 ggcgtgtcgc ttaaaccttg acggccccgc ccgccgccgt gtcgatctgt cggcaccaat
     1681 aatcgaaagt gcctttcacg atcgatggtt gcagcaaata gtcatgtgct tcttgagcaa
     1741 gttcggcggg tacgggaacc aattcgccgt acactctggc tcgcgtctgc atcctggcgg
     1801 ctcgttccag ataaaccgac aagtaggttg cctcttccac ggtgccgccg gttgtcagat
     1861 agccatggtg agccaaaatg atcgaccttt tcgagcctag cgccgccgaa atcagtacac
     1921 cttcctgatc cgcaattggg actcccggcc aatcagctaa aaaggcgcag tcattgtgaa
     1981 agggcgccat gtccatctgg gcaataacca aagtctggcg cgccgccacc aatgccgaaa
     2041 cccaagggga atgggcgtga accatggagc agatgtcggg ccttgcctta tatacccaca
     2101 aatgaaaacg cgtggcggga ttggccatcc cctcgccggt cagtgtctcg agatccggac
     2161 ccacttcgat gaaatcatcg gctgtggctt cgtcgaaccc caagccaaaa cgaagcgtcc
     2221 agtaggcgcc cggacgctcg gagcgtgcac tgatttgccc agccaagcct gcttcctgac
     2281 cagtggcggc cagaattcga catgtatagg caag
//
LOCUS       DATCXU010000038         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_146652,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000038 DATCXU010000000
VERSION     DATCXU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146652"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1090)
                     /locus_tag="VIR04_00785"
     CDS             complement(248..1090)
                     /locus_tag="VIR04_00785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY9459314.1"
                     /translation="MEPLAPSQTAYAVARMRAGHQILDAGNIFSDPYACAILGEQPAE
                     IVAALNLDPNSAAVRTYMARRSRIGEDWLHEAYLRGVRQAVILGAGFDTFSLRNPYSD
                     LAVFEVDHPATQRWKQGRLAAAALSIPSSLKFVPVDFSKDDLTSAMQESGFDKNATAF
                     FLWLGVVPYLHHEAISKTLNSIAAIPGAEVVFDYSEPLENYAPEQRPYIEKMAQRVAA
                     LGEPWLSYFDPVVLAQELKNLGYQEIQDLDRAGIVNWGSDERAVSKVVGPHLIRARRI
                     PESL"
     gene            complement(1156..1611)
                     /locus_tag="VIR04_00790"
     CDS             complement(1156..1611)
                     /locus_tag="VIR04_00790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY9459315.1"
                     /translation="MKPVFALLAAGLFALPMAAHAAPNKHYPSGGSVVSGPSPAYSAA
                     VEANGTLYISGTTDVTDPATGKRPVDASTGAKVVMNNVKRAVEAAGYTMDDIVWLQIM
                     ASDLKDYADFNAVYRTYFKGPLPARAFIGAGSLLGNARFEVMGIAVKAK"
     gene            complement(1644..>2432)
                     /locus_tag="VIR04_00795"
     CDS             complement(1644..>2432)
                     /locus_tag="VIR04_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459316.1"
                     /translation="ASAQGSTDKDKMAAFYKTTGTYWRAHWLDYTKNPEKYADVMALY
                     RKGQAAYPHKPEERDPERYRKDIEAFKDYDAKNALPNNPALFVGSSTIMKWKTAESFP
                     GFSVLNRGFGGSVIGDVDFFYHDVIGKYHPSAILFYCDNDVTGGFSGDVAFAKAMKVY
                     AKVRADFPKVPFVFLSMKHAPNSAFADPGEPKAIDRFNDLAKAKTKTDPLFKYFDMDA
                     PVLDAKGKVQGDLFLDGEHFNDKGYALINPALQKVLVSLGVPHS"
BASE COUNT          516 a          721 c          692 g          503 t
ORIGIN      
        1 cccgccaccc cagatcatga aggcgcacag tcccccgccc acgacctgaa aaatccgctg
       61 atagatcacg cttgcttccc ttggctaagg acgggaggac tttgcctgat gcgctgtcct
      121 catccttcga caagctcagg atgaggaatt tctaagtcct catggtgagc ttgtcgaacc
      181 atgagggtcg cgaatgccct gagcggcagc gtatgggcgc aagaactgcg aacgccgccg
      241 gggagagtta caggctttcg ggtatgcgcc gggcacgaat cagatgaggc cctacaacct
      301 tggacacagc cctctcatca gaaccccaat tcactatgcc tgcccgatcc aggtcttgga
      361 tttcctgata acccaaattt ttcaactcct gggcgagcac gacgggatca aaataagaga
      421 gccaaggctc gcctaacgct gcgactcgtt gcgccatttt ctcgatgtac ggacgctgct
      481 caggcgcata attctcgagc ggctccgaat aatcgaagac aacttcagca ccgggaattg
      541 cagcgattga attgagcgtt ttgcttatgg cctcatggtg cagataagga actacgccga
      601 gccacaggaa gaacgcggtt gcgttcttat cgaatccgga ttcttgcatt gccgatgtca
      661 gatcgtcttt ggaaaaatcg acaggaacaa atttcagact tgaagggata gatagcgcag
      721 cggcagccag cctgccctgc ttccagcgct gggttgcggg atgatctacc tcgaataccg
      781 ctaaatcgga ataaggattt cgcaggctaa aggtgtcgaa gcccgcaccc aaaatcacag
      841 cctgccgaac cccgcgaagg taagcctcgt gaagccaatc ctctcctatc cggctccgcc
      901 tcgccatata ggttctaacc gctgcgctat ttgggtccag attcagggct gcgacgattt
      961 ccgcaggctg ctcgcccaag attgcgcagg catatgggtc cgaaaaaata ttgcccgcat
     1021 cgaggatttg atgccccgct ctcatcctcg ctacggcgta ggcggtttgg ctgggagcta
     1081 acggttccat gactggcaaa attactccag cgtcaatgcg gggcgtatgg accgtaacat
     1141 caggatacgc agcccctact tcgccttcac cgcgatgccc atcacttcaa agcgcgcatt
     1201 gccgagcaag ctcccggcgc cgatgaaggc gcgggcgggc agcgggccct tgaaataggt
     1261 gcgatagacg gcgttgaagt cggcatagtc cttcaggtcc gacgccatga tctgcagcca
     1321 cacaatgtcg tccatggtgt agcccgccgc ctccaccgcc cgcttgacat tgttcatcac
     1381 caccttggcg ccggtcgaag catccaccgg ccttttgccc gtggccgggt cggtcacatc
     1441 ggtggtgccc gatatataaa gcgtgccgtt cgcctccacc gcggcggagt atgcgggcga
     1501 cggcccgctc accacgctgc cgccggaggg ataatgcttg ttgggcgcgg catgcgcggc
     1561 catcggcaag gcgaacaggc cggcggcgag aagagcgaaa acaggtttca tgaaaaactc
     1621 caaaggatga aaatacgccg cctctagcta tggggcacgc ccaacgacac cagcaccttc
     1681 tgcagcgccg gattgatcag ggcatagccc ttgtcgttga aatgctcccc atccaggaac
     1741 aggtcgccct gcaccttgcc cttggcgtcc agcaccggcg catccatgtc gaaatatttg
     1801 aacaagggat cggtcttggt cttcgccttg gccaggtcgt tgaagcggtc gatcgccttg
     1861 ggctcgcccg gatcggcgaa ggccgaattg ggcgcgtgct tcatgctgag gaagacgaag
     1921 ggcaccttgg gaaaatcggc ccgcaccttg gcatagactt tcatcgcctt tgcgaaggcg
     1981 acatcgccgc tgaacccgcc ggtgacgtca ttgtcgcaat aaaacaggat cgcggaggga
     2041 tgatatttgc cgatcacgtc gtgatagaaa aaatccacat ccccgatcac gctgccgccg
     2101 aagccccggt tgagcacgct gaagccggga aaggattccg ccgtcttcca tttcatgatg
     2161 gtggaactgc ccacgaacag ggccgggttg ttcggcagcg cgttcttggc gtcataatcc
     2221 ttgaaggctt cgatatcctt gcgatagcgc tcgggatcgc gctcctccgg cttgtgcgga
     2281 taggcggcct ggcccttgcg gtacagcgcc atcacgtcgg cgtatttttc cgggttcttg
     2341 gtgtaatcca gccaatgggc gcgccaatag gtgccggtgg tcttgtagaa ggccgccatc
     2401 ttgtccttgt cggtgctgcc ttgggcgctg gc
//
LOCUS       DATCXU010000039         4164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_695673,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000039 DATCXU010000000
VERSION     DATCXU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695673"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VIR04_00800"
     CDS             complement(<1..440)
                     /locus_tag="VIR04_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459317.1"
                     /translation="MAIVTVVEPYALLRFGILRHLTDVLPESQVQGVGYSSLSAHAPC
                     NTESDLVLLSVTPYENAAELTQAAQRAYAPKWMLLSSDVATMPDSWQDLPATVAGYVP
                     RKVSPDVLAASIRLVLAGGKCFQSVSCASALAEHVRELPAANEDL"
     gene            complement(457..768)
                     /locus_tag="VIR04_00805"
     CDS             complement(457..768)
                     /locus_tag="VIR04_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY9459318.1"
                     /translation="MTQFRRFPFFFLAVVMALLTGCASTPTQEGTGEYINDTALTTKV
                     KAAVFAEPTLKSAEINVETFKGVVQLSGFVSSSADVNKAVSVARGVDGVKSVKNNMRL
                     K"
     gene            975..1229
                     /locus_tag="VIR04_00810"
     CDS             975..1229
                     /locus_tag="VIR04_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459319.1"
                     /translation="MGNFIAVRNVLLCRGNSSPGKYMNKNQANDQAEQTGDQAKETMG
                     RASGHGIGSRHSEEKRKLQRAIAKAELDFDSLDSDPNNNL"
     gene            complement(1259..1978)
                     /locus_tag="VIR04_00815"
     CDS             complement(1259..1978)
                     /locus_tag="VIR04_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY9459320.1"
                     /translation="MPLITPVSGANRLVAALPRKDRQRFLAHCEPIQLEFAEVLAEPG
                     ERIRHVYFPTDSFISLVTPIDGRASLEVGLVGDEGMLGIFLLLGVDVSPLHALVQGSG
                     PVLRMSAISFRRELGLSPALQRGLKRYLYVILSQLAQTAACTRFHVVEPRLARWLLMT
                     QDRAHSNTFHITHEFLAYMLGVRRAGVTKAATSLQNRKLISYNRGDITILDHKGLEMA
                     SCACYETDKATYSRVMAQRAP"
     gene            complement(2064..2783)
                     /locus_tag="VIR04_00820"
     CDS             complement(2064..2783)
                     /locus_tag="VIR04_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY9459321.1"
                     /translation="MLAPDDPHQNHLLAALPSDDYKRLLPGLEPIPLSLGDVLYESGV
                     QMRSVFFPTTSIVSLLYVMEDGASAEIAVVGREGIVGVSLFMGGETTPSRAVVQSAGQ
                     AYRLKGQRLKDEFNRNGPMQHLLLRYTQSLLTQMAQTAVCNRHHSLDQQLCRWLLLSL
                     DRLGSNELIMTQELIANMLGVRREGVTEAAGNLQRAGLIEYRRGRITVLDRPGLEARA
                     CECYAVVKTECDRLLPKAMAS"
     gene            2888..3457
                     /locus_tag="VIR04_00825"
     CDS             2888..3457
                     /locus_tag="VIR04_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459322.1"
                     /translation="MPALAAPTKQGEIYVLNQSTGKPLLPVTEEPAPQGAAKGDHTSP
                     TQPVSALSYNPRTLVGADMWGATMFDQLACRIAFTKMRYGGRFTPPSTQVSLIYPGNF
                     GVFNWGGIAVDPQRQVAFTTPTDLVFVSQLVARKNDKTLYVQGTNIPKGSFIAEVARS
                     QVDSWSPSKPDAATDFRAIAAKYGMQILT"
     gene            complement(3776..4000)
                     /locus_tag="VIR04_00830"
     CDS             complement(3776..4000)
                     /locus_tag="VIR04_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEY9459323.1"
                     /translation="MTTKFKVGDHVGWNSEAGQVSGKIIKVHTKETDYKGQKRHASAE
                     DPQYEIKSDKTEHVAMHKGSALHKVKSAKS"
     gene            complement(3984..>4164)
                     /locus_tag="VIR04_00835"
     CDS             complement(3984..>4164)
                     /locus_tag="VIR04_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459324.1"
                     /translation="MTEQVEPYGIPQHCSVLQRRDQSASRFNIDPGCPRRFRAKFVKT
                     GSESAQIGDQCDYKI"
BASE COUNT          977 a         1149 c         1117 g          921 t
ORIGIN      
        1 aggtcttcgt tcgccgcggg aagttcacgc acgtgctccg ccagtgcgga ggcgcacgac
       61 accgattgaa agcactttcc tccagccagc acgagtctga tcgatgcggc taatacatcc
      121 ggcgacactt tccttggcac atagcccgcg accgttgctg gcaggtcttg ccaggaatcg
      181 ggcatggttg cgacgtcgga cgacaacaac atccattttg gcgcataggc gcgttgtgcc
      241 gcctgagtca gttcagccgc gttttcgtac ggggtaaccg atagcaacac caagtcgctt
      301 tcggtgttgc agggcgcatg cgccgaaaga gaggaatagc ccactccttg aacctgcgat
      361 tcgggcaata catcagttaa atgcctcaag atgccgaagc gaagcaacgc atagggttca
      421 accactgtga cgatagccat ttttttctcg gctgcgttac ttgagccgca tattattttt
      481 gactgatttc acgccgtcga cgccgcgtgc gacgcttact gctttgttga catccgcgct
      541 agagctaaca aagccgctca gttgcaccac ccccttgaag gtttcaacat tgatttcggc
      601 tgatttcaaa gtaggctcgg caaagactgc tgccttaacc ttggtggtca aagccgtatc
      661 attgatatat tcacctgtgc cttcttgcgt tggcgtagac gcgcagcccg tcaaaagcgc
      721 catcacaacg gctaggaaga aaaatgggaa acgtctaaat tgtgtcatga tcgcattatc
      781 caaaaagatg agaactacat ggctcgacag cggtgcgcgt cgcttaccct gtaactacaa
      841 gtatcgttca gagtttgata gttatctgta cgctaccgca cttataagct atcgcacttg
      901 cgaatcgtgt ccggcatgat tagtgcgcgc gaaacccagg tatgtgcgtt aacgcacgga
      961 attcggggcg gaaaatgggt aacttcattg ctgtacgcaa cgtgctgctt tgtcgcggaa
     1021 atagctcacc agggaaatac atgaacaaaa atcaagccaa tgatcaggct gaacaaacgg
     1081 gcgaccaagc aaaggaaacc atgggtcgag ctagtggcca tggcatcggc agcaggcatt
     1141 ccgaagaaaa gcgcaagctt cagagagcaa tcgctaaagc cgagctggat tttgacagtc
     1201 ttgatagcga tcccaataac aacctgtaat ttcctcgtta gcgggcgatg gacgcgagct
     1261 atggggcgcg ctgggccata acgcgagagt aggtggcttt gtcggtttcg tagcacgcgc
     1321 acgaagccat ttcgaggccc ttgtggtcaa gaattgtaat atcgccgcgg ttataactga
     1381 tcagtttgcg attttgtagc gaagtggccg ccttggtaac gccggcccga cgcacaccca
     1441 gcatataagc caggaattca tgcgtaatat gaaacgtgtt tgagtgcgcc cggtcttgcg
     1501 tcatcaagag ccagcgcgca agacgcggtt cgacgacgtg aaagcgggtg caggcggctg
     1561 tctgtgcgag ctggctcagg attacataga gataacgttt caggcctcgc tgcagggctg
     1621 gactaagccc aagttcacgg cgaaatgaga ttgcactcat acgtaatacc gggccagagc
     1681 cctgcaccag ggcatgtagc ggcgagacat ccacccccaa taagaggaaa atgcccagca
     1741 tgccctcgtc accgaccaaa cccacctcca ggcttgcgcg gccatcgatc ggcgtcacca
     1801 gggaaataaa gctgtccgtc ggaaagtaaa catggcgaat gcgttcgccc ggctcggcaa
     1861 gaacttcggc aaattccaat tggattggtt cgcagtgtgc aagaaagcgc tggcgatctt
     1921 tgcgcggcaa tgccgcgacc aaacggttag cgcccgagac gggcgtgatc agtggcatga
     1981 gaccctctgg atacttcatt caccgcagtc tgaggcaaag atcaatgtcc gtctggacgg
     2041 tagcgcacta aggaaggcgc ggatcaggaa gccattgcct tcggaagcaa acgatcgcac
     2101 tccgtcttga ccacggcgta gcattcgcag gcccgcgctt ccaggcccgg ccggtcgagc
     2161 accgtgatgc gaccgcggcg atactcgatc aggcctgctc tttgcaggtt tccggccgct
     2221 tccgtgacgc cttcgcgacg caccccaagc atattggcga tgagctcctg cgtcatgatc
     2281 agctcgttcg agcccaagcg gtccagactt agcagcaacc agcggcatag ctgctggtcc
     2341 aatgaatgat gacggttgca caccgccgtc tgcgccatct gcgtcaacaa tgactgggtg
     2401 tagcgcagca ataggtgctg catcggaccg ttgcgattaa attcatcctt cagccgttgt
     2461 cccttcagcc gataagcctg accagcgctt tgcacgacag ctcgactagg cgtagtctcg
     2521 ccgcccataa aaagggagac gccaacgatt ccttctctgc ccacgacggc gatttcggcc
     2581 gacgctccgt cctccataac gtaaagcaga gacacgattg aagtcgtcgg gaaaaagacc
     2641 gaacgcatct gtacacccga ttcgtaaagc acatcgccga gcgatagcgg tatcggttcc
     2701 agaccgggca acagacgctt gtagtcgtcc gacggcaagg cggcaagcaa gtggttctga
     2761 tgagggtcat cgggagcaag catgagcaat tagttttctt attcgagagt tctcattata
     2821 gggtgctatc ccatcctacg ttcccgcaca acctagcctt atcgatctga caatcaatgg
     2881 gcagatcgtg cccgctttgg cggcgcctac caaacaaggc gaaatttacg tgcttaatca
     2941 aagcaccggc aaaccgttgt tgccggttac ggaagagccg gcgcctcagg gtgcggctaa
     3001 aggcgaccac acatctccga cccagccggt ctcggcgcta tcgtacaacc cgcgaactct
     3061 ggtgggcgcc gacatgtggg gcgcgacgat gttcgatcag cttgcatgtc gcatcgcgtt
     3121 cacgaaaatg cgttacggtg gccgatttac cccgccgtcc actcaagttt cgttgatata
     3181 tccgggtaac tttggagttt tcaactgggg cgggattgcg gttgatccac aacgtcaggt
     3241 ggccttcacc accccgaccg atctggtttt cgtgtcgcaa ctggtggcgc gtaaaaatga
     3301 caaaacactt tatgtacagg gtacaaatat tccgaaaggc agtttcattg ctgaagtggc
     3361 caggtcacag gttgatagct ggagcccttc gaagccggat gcggcaacgg acttccgcgc
     3421 cattgcggcg aagtatggaa tgcagattct gacctaatgc gcaacgtgaa accagcactg
     3481 tgacgcccat ccctgcattc aggctcagat attttggacg ccaaggtgcg gaataaactg
     3541 aaccgggcat gtcacacaaa atggccagaa gcgtctttgt tggcgtcgcg agcattctcg
     3601 acatcacctc tagcattatc gcgggcctga ggatgatgcg gatcaagggt caatcggaac
     3661 tcttccatca caccgacgcc aaccactaga tcccgatgac cctcagcggc gccagcgaac
     3721 ttcgattccg tggccttcag tttgcatcca agcaccactt acgaagttag cgccgtcacg
     3781 atttagctga tttgacctta tgcagcgccg aacccttatg cattgcgaca tgttcggttt
     3841 tatcactctt gatttcatat tgcggatcct ctgcgctggc atggcgcttc tgtcccttgt
     3901 aatcagtctc tttcgtatgg accttgatga ttttgccgct aacctggccg gcttcagaat
     3961 tccagcccac atgatcgccc actttaaatt ttgtagtcac attgatcacc tatttgtgcg
     4021 gattcactac ccgtcttgac aaacttagca cgaaagcgcc gcgggcagcc gggatcgatg
     4081 ttgaaacgag acgcgctctg gtcgcgacgt tgcaatacgc tgcaatgttg gggaatgccg
     4141 tatggttcaa cctgttctgt catc
//
LOCUS       DATCXU010000040         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_303582,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000040 DATCXU010000000
VERSION     DATCXU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303582"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VIR04_00840"
     CDS             complement(<1..489)
                     /locus_tag="VIR04_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY9459325.1"
                     /translation="MGITGESRKFAPRPVLIILACSVALAAVAASASGAVNIPWSRFP
                     QLLWRGADSGDMALHNVLFQIRLPRVLFSMVTGAALAATGVAMQALFRNPLAEPGLVG
                     ISSGAALGAVCAITMAASSFFAVAGAAFLGGLLSTFIAYSVGCRHRGVAGLLLAGVAI
                     NAI"
     gene            604..1056
                     /locus_tag="VIR04_00845"
     CDS             604..1056
                     /locus_tag="VIR04_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459326.1"
                     /translation="MTLPLDFLKDLGRRHSDEIAIGFDVDQVTYAELAIAVNALAVAL
                     QMKDPTPGSAVGLCAAHSLEYVVSVMAISAAGKVLLPMNCQGNSEELYNILSETHPSA
                     VIVDDLGDKQIHSDPDLKIHFSQFPGLVLTYRDHLPVDYSRSPGSAAD"
     gene            complement(1097..>2043)
                     /locus_tag="VIR04_00850"
     CDS             complement(1097..>2043)
                     /locus_tag="VIR04_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY9459327.1"
                     /translation="IKSLAWPPLPVAAWSDTRDTLHMWLQIVGKTRLALAPMENHWWQ
                     VVFYITSRGLSTSPMPYGQRAFTVDFDFIDHVLLISASDGGTHQFPLGPYSVADFYAK
                     YQAALRLLDFNIAIMPSPVEVVTAIPFEKDHQHASYDPDAVHRFWRILSQTDRVLKQF
                     RGRFLGKSSPVHFFWGSLDIAMTRFSGRSAPVHPGGAPNCPDYVMVEAYSRECASCGF
                     WPGGGAFAEPAFYAYAYPQPAGYADWPANPGGARYSNEAQEFILPYDVIRQLPEPDEA
                     LLEFFQSTYDSAAELGQWDRATLDRPKAQWFTRPAATR"
BASE COUNT          465 a          592 c          580 g          406 t
ORIGIN      
        1 gattgcgttg atggccacgc cggccagcag caggcccgcc acaccgcgat ggcggcaacc
       61 gactgaataa gcgatgaaag tggaaagcaa gccgcccaga aaggcagcgc cggcgacggc
      121 gaaaaagctg cttgccgcca tcgttatggc gcaaacggcg cccagcgcgg cacctgacga
      181 gatgcccacc aggccgggtt cggccaaagg attgcgaaac aaagcctgca tggcaacgcc
      241 ggtggcggcc agcgccgcgc ccgtcaccat actgaacaaa acacgcggca gtcgtatctg
      301 aaacagcaca ttgtgcagtg ccatatcgcc ggaatcggcg ccgcgccata atagttgagg
      361 aaaccggctc cagggaatgt tcacggcgcc gcttgcgctg gctgccacag ccgccaatgc
      421 cacgctgcac gccagtatga tcagaaccgg acggggcgcg aactttcgtg attcacccgt
      481 aattcccatt attaccgccc gatggccttg ccgtcaccgc ccaagtttac caccgcccac
      541 ctggctgttc ttcgcgtaca cttcctgttg gctgccatta cgataaattg aaaatccact
      601 gatatgacac ttccacttga tttcctgaag gacttgggtc ggcggcacag cgatgaaatc
      661 gccatcggct tcgacgtcga ccaagtcacc tatgccgaac tggcaatcgc cgtgaacgca
      721 cttgcggtgg cgttgcaaat gaaagatccg acgcccggat ctgcagtggg cctttgtgcg
      781 gctcattcgc tggagtacgt ggttagcgtg atggccatat cggccgcagg caaagtttta
      841 ttgccgatga actgccaggg taattctgaa gaactgtaca acatacttag tgaaacgcat
      901 ccgtcggcgg tcattgtgga tgatcttggc gacaagcaaa ttcacagcga cccggatttg
      961 aaaattcact tctcgcaatt ccccgggctg gttctcactt atcgtgatca tctgccagtc
     1021 gattattctc gttcccccgg ctccgccgcg gactaaattt tgttttttta aatcgccgcg
     1081 gcccagcgtt ggcggctcag cgcgtggccg cgggtcgcgt gaaccattgt gcttttggcc
     1141 gatcgagcgt ggcccgatcc cactgaccga gttcagccgc ggaatcatat gtcgattgaa
     1201 aaaactccag caacgcttca tcaggttcgg gcaattggcg aatcacgtca tagggaagga
     1261 tgaattcctg agcctcgttg ctatagcgcg caccgcccgg gtttgccggc caatctgcgt
     1321 atcccgccgg ttgcggatag gcataggcgt agaatgcggg ctcggcaaac gcgccgccgc
     1381 caggccaaaa gccgcaactg gcgcattcgc gggaataggc ctcaaccatc acgtaatcgg
     1441 ggcaattggg agcgccgccc gggtggaccg gcgcacttcg tcccgaaaat cgagtcattg
     1501 cgatgtcgag gctgccccaa aaaaaatgca cgggactgct ctttccaaga aaacggccgc
     1561 gaaattgttt taacacacgg tctgtctgag aaagaatccg ccagaatcga tgcacggcgt
     1621 ctgggtcata ggatgcgtgc tgatggtctt tttcgaacgg aatggccgtc acgacctcga
     1681 ccggcgacgg catgatcgca atgttgaagt ccagcaatcg caaggctgct tgatatttcg
     1741 catagaagtc cgcgaccgag tatggcccca gagggaattg atgtgttccc ccgtcgcttg
     1801 cggaaataag aagcacatgg tcgataaaat cgaagtcgac cgtaaacgcg cgctgaccat
     1861 aaggcattgg cgaggtggaa aggccgcgcg aagtgatgta gaacacgacc tgccaccagt
     1921 gattttccat gggcgccaac gcaaggcgtg tcttgccgac aatttgcagc cacatatgga
     1981 gcgtgtcgcg cgtatctgac catgcagcca caggcagcgg cggccacgcc aggctcttta
     2041 tgg
//
LOCUS       DATCXU010000041         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_460338,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000041 DATCXU010000000
VERSION     DATCXU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460338"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VIR04_00855"
     CDS             <1..409
                     /locus_tag="VIR04_00855"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY9459328.1"
                     /translation="TTLSPSLALKDGQPYMVFGTPGGDQQDQWPLAFFLRHILMGMNL
                     QEAIEAPSWHINHFPGSFWPRTTTLNRITLESRFNEACISGLKAAGYEVKVGAPWSEG
                     RISACTREFTKDGNILLRAAANPRGMQGYAVGR"
     gene            442..1509
                     /locus_tag="VIR04_00860"
     CDS             442..1509
                     /locus_tag="VIR04_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9459329.1"
                     /translation="MIKRREFLKMSSRAATGLALAGTSSLALAQSRPASMAMMTWGGL
                     WGNGMAQYVDTPFTKETGIKIIQDRGSSPVERVTKLKINLNNQIYDLMQVHDGVVPLA
                     EKQGVVEELDPNSANLPFLKEIPESFRRPGWVACIYSTLGIVYNHDVIKTPPKGFADL
                     WNDEYRGRIVLPSITHSIGPYIIPIGAMAAGKDSKDADTGFSMLRKMVKLDPIWARDT
                     DTIMSSLLSGEAVIGLLYKSQTYTVKDKGAKVDWVFPSEGAISYLAGTSIAKNTKNKS
                     FAEKYVNMTLDPQHQLWVTKVFNYAGTNPRMLKMLPPSLQERVHYSDAEIARIINLDQ
                     DFISGNRAKWTDEWNRIVAGG"
     gene            1513..>2085
                     /locus_tag="VIR04_00865"
     CDS             1513..>2085
                     /locus_tag="VIR04_00865"
                     /inference="COORDINATES: protein motif:HMM:NF021354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HEY9459330.1"
                     /translation="MACQLSWAHRPDNLKIAVLAHRTAVHGRPVSLTQDGYAVPDSLA
                     AAAQSLGLQVSAQTTSKNVSPLRMPRIVLFCGASIGYPYYAYYAHSLLSLGLIYSHAG
                     AADITGGILNDADVLIIPGGFANWGLDRAESHGGVDQAIELFLKNGGALIGSCGGAYY
                     ASQGRPGWLGLIDAKPVFTHEYLQTGAAVVN"
BASE COUNT          495 a          594 c          568 g          428 t
ORIGIN      
        1 caccaccctc tcgcccagcc tggctctgaa agatggccag ccgtacatgg tatttggcac
       61 cccaggcgga gaccaacagg accaatggcc gctcgccttc tttttgcggc acatcttgat
      121 gggcatgaac ctgcaagagg cgatcgaagc gccatcctgg catatcaatc atttccccgg
      181 ctcgttctgg ccacgcacga caacactgaa ccgcattacc ttggaatccc gcttcaacga
      241 agcctgcata tctggcctga aggcggccgg gtatgaggtg aaagtcgggg ctccctggtc
      301 ggaaggacga atcagtgcct gcacgcgcga gtttacgaaa gatggcaaca tactgctgcg
      361 cgccgcggca aatccgcgcg gcatgcaagg ctatgcggtc ggccgctaga atgcggcaca
      421 ttacaaccga aggagcactc tgtgatcaaa cgccgtgaat tcttgaaaat gtcgtctcgt
      481 gccgccaccg ggcttgctct tgccggcacc tcttcgcttg ccttggccca atccaggccc
      541 gcttcaatgg cgatgatgac ctggggtgga ttgtggggca acggtatggc ccaatatgtg
      601 gataccccgt tcaccaaaga aacaggaatc aagatcatcc aggaccgcgg ttcaagtcca
      661 gtggaaaggg taaccaaatt aaagatcaat ctcaataatc agatatacga tttaatgcag
      721 gtgcatgatg gcgtcgtccc cctggctgaa aaacagggcg tggtggaaga gcttgacccg
      781 aactccgcga accttccctt tctgaaggaa ataccggaaa gctttcggcg gcccggctgg
      841 gtggcctgca tttattcgac acttggtatt gtctataacc acgacgtaat caaaacgccg
      901 cccaagggtt ttgccgacct ctggaacgac gaatatcgcg gccgtattgt gctgccctcc
      961 attacgcact ctattgggcc ctacatcatt cccattggcg ccatggccgc gggcaaagat
     1021 tccaaagatg ccgataccgg tttcagcatg ctaagaaaaa tggtcaagct ggatccgata
     1081 tgggcccgcg acaccgacac catcatgagc tcgctgctta gcggtgaagc ggttatcggg
     1141 ttactttata aatcgcaaac ctatacggtc aaagacaaag gggcgaaagt cgactgggtc
     1201 tttccgtcgg agggcgcaat ttcctatctg gccggcacca gcatcgcaaa aaacacaaaa
     1261 aacaaatcgt tcgctgaaaa atacgtgaac atgactcttg atccgcaaca tcagttgtgg
     1321 gtgacaaagg tgttcaacta cgccggcacg aacccgcgca tgttgaaaat gctgccgcca
     1381 agcctgcagg aacgcgtgca ttattccgac gccgaaattg cacgcattat caacttggat
     1441 caagatttca tttccggcaa cagagccaaa tggaccgacg aatggaacag gatcgtcgca
     1501 ggcggttaag gcgtggcatg tcaactttcc tgggcgcatc gccccgacaa tctgaagatt
     1561 gccgtgctgg cgcaccggac ggccgtgcat ggccgcccgg tcagtctgac acaggatggt
     1621 tatgcggttc ccgacagcct ggcggcggcg gcacagagcc tgggcctgca ggtctcggcg
     1681 caaacaactt ccaaaaatgt gtcgccgctg cgaatgccgc gcattgtgct tttttgcggc
     1741 gccagcatcg gctaccctta ctacgcctac tatgcacaca gcctattaag cctggggctg
     1801 atctatagtc acgcgggcgc ggccgatatt accggcggca tcctcaacga tgcagatgta
     1861 ctgatcattc cgggcggatt tgccaactgg ggtctggaca gagccgagag ccacggcggt
     1921 gtcgaccagg cgatagaact atttctaaaa aatggcggcg ccctgattgg ctcatgcggc
     1981 ggcgcctact acgcatcgca aggccgtccg ggctggctcg gactaattga cgccaagccc
     2041 gtgttcaccc atgaatacct gcaaactggc gctgcggtag ttaat
//
LOCUS       DATCXU010000042         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_853749,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000042 DATCXU010000000
VERSION     DATCXU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853749"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..390
                     /locus_tag="VIR04_00870"
     CDS             124..390
                     /locus_tag="VIR04_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY9459331.1"
                     /translation="MSTLDRLQHMLAADYQIPQTRSAPSAALEDLGMDSLGVMELLFK
                     VEDEFHIHVPTTQTSLLTIQDVVDFIDQLVKTQNAELGSGSRSN"
     gene            408..1646
                     /locus_tag="VIR04_00875"
     CDS             408..1646
                     /locus_tag="VIR04_00875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY9459332.1"
                     /translation="MRRVAISGLGIISPLGNSAAEMLANARAGHSGIRRMDDSFCPRL
                     ATRIAAIADFDGTHYFEAPKFRMLDKSAQFALVATRQALADARLDLAGADVSRIGVFV
                     GSAMGGAQSCNDSYKALYGDCQDRINPFSVLLAMNNGAASWIAIDHGITGPNLTYSTA
                     CSSSAVAIGEAGKRIQGGEVDLMIAGGTEAPLSFGPLKAWEALRTLATEDSRNPSGSC
                     KPFSKNRTGMVLGEGAAILVLEEWERAIERRAPVYGELVGYGLSTDTQHLTRPSVEGQ
                     AQAMRLALLSAGLAPDAISYLNAHGTGTLANDPVETAAIKAVFDSYAYCLPVSSSKSM
                     HGHLLGAAGALEFALAILAMQEGVIPPTINLDEADPECDLDYVPNQARPAVGMNAIMS
                     NSFAFGGTNAVLIARKAETE"
     gene            1707..>2038
                     /locus_tag="VIR04_00880"
     CDS             1707..>2038
                     /locus_tag="VIR04_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY9459333.1"
                     /translation="MEYRQYRQYRPVATAITAATFSVTLAACAVGPDYRTPAPPAGDR
                     VTAEPMPEQTVAAPGPAGAAQRFVAQHDIPGQWWALFHCAPLNALIHEALSNSPTLVA
                     ARAVLRQA"
BASE COUNT          411 a          563 c          623 g          441 t
ORIGIN      
        1 ccgccgctgg tggctgcctt tcttcccata ttgcagcgcc acccgtcgct tctgacgtat
       61 gcggcccgaa ggagtggtaa agtcgactgc ataccctgca atgggatagc gagagagtaa
      121 aaaatgagca ctcttgatcg actacagcac atgttggccg cggactacca gattccgcag
      181 accagatcgg cgccttcggc cgcacttgaa gacctgggga tggattccct aggggtgatg
      241 gagttgttat ttaaagtcga agacgaattc catattcacg tgccaacgac tcagacgtcg
      301 ttattaacga tacaggatgt cgtggatttt atcgatcagc tcgttaaaac gcagaacgcg
      361 gagctcggat cggggagcag atccaactaa tttttgacgg gggccttttg agacgtgtcg
      421 cgattagcgg tcttgggatt atttccccct tgggcaactc cgccgccgag atgctggcca
      481 atgcgcgcgc cggccattcg ggcattcgac gcatggatga tagtttttgt ccgcgcctgg
      541 caacccgcat cgcggcaatc gccgattttg acggaacaca ttattttgaa gcgcccaagt
      601 ttcgcatgct ggacaagagc gctcagtttg ctttggttgc aacgcggcag gctctggccg
      661 atgcgcgtct cgatctggcc ggtgccgatg tttcgcgcat tggcgtattt gtgggtagcg
      721 ccatgggcgg ggcacaatcc tgcaacgata gttacaaagc cctgtatggc gattgccagg
      781 accgcatcaa tcctttcagc gtgctgctcg cgatgaataa cggcgcggca tcgtggattg
      841 ccatcgacca cggcatcacg ggacccaatc ttacctacag cacggcctgt tcctcttcgg
      901 ccgtcgccat cggtgaagcc ggcaagcgca ttcagggggg cgaagtcgac cttatgattg
      961 cgggagggac ggaagcgccg ctttcgttcg gccctttaaa ggcgtgggaa gctctgcgca
     1021 cgctggcgac ggaagattcg cggaacccgt cgggatcgtg caagccgttt tcaaaaaatc
     1081 ggaccggcat ggtgcttggc gaaggcgccg ccatcctggt gcttgaagag tgggaaaggg
     1141 cgattgagcg cagggctccc gtgtatggag aacttgttgg ctatggcctg agcaccgaca
     1201 cccaacatct gacgcggcct tcggtcgaag gccaggcgca agccatgcgg ctggctctgc
     1261 tttcggcggg tctggcgccg gacgccatat cgtatttgaa tgcgcatggc actggcacgc
     1321 tggctaacga tcccgtcgaa acggcggcga ttaaagcggt atttgattca tatgcctatt
     1381 gccttccggt aagctcaagc aaatcgatgc atggacattt gcttggcgct gcgggggctc
     1441 tggaatttgc ccttgccatt ctggcgatgc aagaaggcgt gatccctccg acgattaatc
     1501 tggacgaagc ggatcccgaa tgcgatttgg attatgtgcc taaccaggcg cgcccggcgg
     1561 tcggcatgaa tgccatcatg tcgaactcgt ttgcctttgg gggaaccaat gcggtattga
     1621 ttgcgaggaa agctgaaacg gaatgaatgc gtgcctgcac gaacgagctt ttggcgtgat
     1681 attcttgaaa acgtttcgga gatacagtgg aatatcggca ataccggcaa tacaggccag
     1741 tagcaacggc gataaccgcc gctactttca gcgtaactct ggccgcctgt gctgtggggc
     1801 ccgattaccg cacgcccgcg ccgccagcag gcgatcgcgt gactgccgag cccatgccgg
     1861 agcagaccgt cgcggcgccg ggcccggcgg gggccgcgca gcgctttgtc gcgcagcacg
     1921 atatacccgg ccaatggtgg gcgctctttc attgcgcgcc gcttaatgcg ttgattcatg
     1981 aagcgctctc caacagccca acgcttgtgg cggcgcgcgc cgtcttgcga caggcgca
//
LOCUS       DATCXU010000043         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_700233,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000043 DATCXU010000000
VERSION     DATCXU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700233"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VIR04_00885"
     CDS             <1..1220
                     /locus_tag="VIR04_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY9459334.1"
                     /translation="DVVNAGRPLLWQHLFWMFGHPWVYVVVLPAMGIVSDALPTFCRR
                     PLAGYTPVAIATVATMVIGFVVWVHHMFASGIAPVALSFFGATSTIISIPSAVATFAW
                     IATIWLGRPVYRVPFLFFFGFVLLFVIGGVSGVMTAAVPFDWQLTGTYFVVAHLHYVL
                     LGINVFPVLGGIYYWFPKFTGRMTSEVLGKFSFWVTFIGFNVAFFPMHISGLLGMPRR
                     IYTYSPEMGWSTTNLITSAGSFIFALGILIFLVDIAVSLRHGKPSGNNPWDGATLEWA
                     VSSPPPPYNFAVVPSVASRNPLWEDRIGEPPFSVLDTGFLLDHGREALGTTPLDAQPD
                     IILKMPTDSYAPFMLAVVSALLLVAMLLHAWTATGLMAAACGVTTLAWLWPERLLIQR
                     EPQPVHDAGDHNE"
     gene            1288..1782
                     /locus_tag="VIR04_00890"
                     /pseudo
     CDS             1288..1782
                     /locus_tag="VIR04_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
     gene            1780..3066
                     /locus_tag="VIR04_00895"
     CDS             1780..3066
                     /locus_tag="VIR04_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY9459335.1"
                     /translation="MTLYVTPFANAQAATPAAGTATAFSDPTLIARGAYLAKLADCSA
                     CHTSTTKGSAPFAGGLGMGSPLGTIYSSNITPDPKTGIGQYSYADFDRALREGVTPTG
                     KRLYPAMPYPSFTKMSDADMRALYAYFMHGVQPVSAQPPETKLPFPFNQRWGLFVWDL
                     AFVRHDRYMPNGVNSAQWNRGAYIVQSLGHCGACHTPRGIAYEERGYDESSRLYLTGG
                     VNDNWFAPNLTGDPGSGLGRLSERDIVSFLKSGHGKQVHIVTFGSMTQVVEDSAQHFS
                     EDDLKAVAIYLKSLPAREPSGAYEPDSLKARQADSAVKTGDIQRPGSGLYMSSCVKCH
                     KADGHGEFEKYPSLAGNPAVLAPDTTSMIRMVMQGGESPQTLTGPKPRKMPAFASKLT
                     DTEIARVLTFVRTTWGNQASPVLTRDVAALRARLEK"
     gene            complement(3081..3524)
                     /locus_tag="VIR04_00900"
     CDS             complement(3081..3524)
                     /locus_tag="VIR04_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY9459336.1"
                     /translation="MRTPASIAKHPIHPMLVVFPIGLWVFSFICDLIGLGAAAPTVWH
                     TVAFYTMVGGVIGALLAAVPGLIDLIYYKGATPPIKKLAVTHMVINLVAVVLYLVNVW
                     IRAGSPASMKTPIILSVIGVSLIGVSGWLGGQMVHVYGVGVEGRE"
     gene            3664..4143
                     /locus_tag="VIR04_00905"
     CDS             3664..4143
                     /locus_tag="VIR04_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY9459337.1"
                     /translation="MNKSEIPTAQKNVLSLLLDDHRKVKKIFNDFKTEKNTAKKQSMV
                     AEACTELTVHTQIEEQKFYPFLRDQEPAVFGDLLDEALVEHATAKELIRQLREAGPPD
                     DLYEAKFTVLGEYVNHHVTEEENELFPKVISKKVNLQDLQPEIEQLKKELMSKASKS"
     gene            4228..>4663
                     /locus_tag="VIR04_00910"
     CDS             4228..>4663
                     /locus_tag="VIR04_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459338.1"
                     /translation="MAAYPEARPPADLSDSNTSGVSWAAIFAGAAAAAALSLILVILG
                     FGLGLSAVSPWAYAGAGALTIGIATAVWLAAMQIIASGLGGYIAGRLRVKWPGLHTDE
                     VYFRDTAHGMLSWAIATLVTAAFMGSALTGIVSGGAELAGNVA"
BASE COUNT          983 a         1311 c         1294 g         1075 t
ORIGIN      
        1 ttgatgtcgt caacgcgggc cggcctttgc tatggcaaca tctattctgg atgttcggac
       61 atccgtgggt gtacgtcgtc gtgctgccgg cgatgggaat cgtctctgat gcgttgccaa
      121 ctttctgccg gcggccgctt gcaggataca cgccggtcgc tattgcgacc gtggctacga
      181 tggtcattgg gttcgtcgtt tgggtccatc atatgttcgc aagcggtatt gcgcctgtgg
      241 ccctttcgtt ttttggcgcc accagtacga tcatttcgat ccccagcgcg gtcgccacct
      301 tcgcatggat cgccacaatt tggctcggtc gcccggtgta tcgagtgcct ttcctgtttt
      361 ttttcggttt cgtcctgctg tttgtcattg gcggagtttc gggcgtgatg acggccgcgg
      421 tgccgttcga ctggcagctt accggcactt actttgtcgt ggcgcatctg cattatgtct
      481 tgctcgggat caacgttttt cccgttttgg gcggcattta ttactggttc cccaaattta
      541 cgggaaggat gaccagcgag gtgctgggga aattttcgtt ctgggttacg tttatcgggt
      601 tcaacgtcgc ctttttcccg atgcacattt caggcctgct gggtatgccg cgccggattt
      661 atacctactc accagaaatg ggatggagca ccaccaacct gattacctct gcaggatcat
      721 ttattttcgc cttgggcatc ctgatctttc ttgtcgacat agccgtgagc ctgcgtcacg
      781 gcaagccatc cggcaataat ccctgggacg gtgcgacgct cgaatgggcg gtatcttcgc
      841 cgccgccgcc gtacaatttc gctgtcgtac cgtcagtggc cagtcgcaat cccctgtggg
      901 aagaccgcat tggcgaacct cctttttctg ttcttgacac aggctttctg ctcgatcatg
      961 gccgggaagc actgggcaca acaccactgg acgctcagcc cgacatcatt ctgaaaatgc
     1021 ccactgactc ttacgcgcca ttcatgctgg cggtagtcag cgcactgctg ctggtcgcca
     1081 tgctgctgca tgcatggacc gccaccggac ttatggccgc ggcctgcggc gttacgactt
     1141 tggcgtggct gtggcccgaa cgcctgttga ttcagcgtga accgcagccc gtacatgatg
     1201 ctggagatca taatgagtga aacggcggac accagtcaaa tttttccagt cggcagcgcg
     1261 ggcgagctat ccagcggatg gtggggcatg aagacgctga tcattactga agcgtcatta
     1321 tttgtctatt tgattttttc atacttgtat ctggcgtccc agacgccgca gcactggcca
     1381 cccgaaggat tacccatgat cggcatcgga gcatttaaca cgagtattct attttccagc
     1441 agcgtattcg tgtggctctg cgagcgtttg gtgcggcgtc gaaaaattaa tcgggcgatc
     1501 gcatcgatgg gattggcgat tctgctcggg gtcgtcttcg taggcattca actaaaggag
     1561 tggcacgccc atccctacgg aatcgcgacg catctgtacg gatcgctgta cttcaccatc
     1621 acgggctttc acgtcattca tgtgctcgtc gggctcatcg tactgacgtt cttgctgctc
     1681 tggatcgctc tgggctactt cgacgaaaag cgctgcgccg cgctcaaagt tggcggcctg
     1741 tactggcatt ttgttgatgt cgtgtggatt ttcatcttta taacgcttta cgttacgcct
     1801 tttgccaatg cgcaggcggc aacgccagcg gccggcacgg caaccgcatt ctctgatccc
     1861 acattaattg cgcgcggcgc ctatctcgcc aagcttgccg actgcagcgc ctgtcacacc
     1921 tccaccacca agggaagcgc accatttgcc ggcggccttg gcatgggatc accattgggc
     1981 acgatttatt ccagcaacat aacgcccgac ccgaaaaccg gcattggcca gtatagctac
     2041 gccgattttg accgggcatt gcgcgagggc gtgacgccca ccggcaaacg actttacccg
     2101 gccatgccgt atccgtcctt caccaagatg agcgacgcgg acatgcgtgc gttgtatgcc
     2161 tatttcatgc acggggtgca acccgtcagc gcacagcccc cagaaacaaa gctgccattt
     2221 ccattcaacc agcgctgggg tctgttcgtt tgggatcttg cgttcgtgcg ccatgaccgc
     2281 tacatgccaa acggcgtaaa cagcgcgcag tggaatcgcg gcgcctacat cgtacagtca
     2341 ctgggccact gcggcgcctg ccacacgccg cgtggaatcg cctatgaaga acgaggatac
     2401 gatgaatcgt ccaggctata tctgacgggc ggcgtcaacg ataactggtt cgcgccgaac
     2461 ctgacgggcg atccgggatc aggtcttggc agactgtccg agcgtgacat cgtatccttt
     2521 ctaaagtcgg gccacggcaa gcaggtgcat atcgtcacgt tcggcagcat gacacaggtg
     2581 gtagaagata gtgcgcaaca tttcagcgaa gacgatctga aggccgttgc aatctacctg
     2641 aagagccttc ccgcacgtga gccgtcgggt gcatacgaac ctgacagcct gaaggcgcgg
     2701 caggctgact cggccgtgaa aacaggtgat attcagcgac ctggatcggg tctttacatg
     2761 tcttcttgtg taaagtgcca caaagccgac gggcatggcg aattcgagaa atacccgtca
     2821 ctcgcaggga atccggcggt gctggctccc gataccacat caatgatccg gatggtcatg
     2881 caaggcggcg aaagccccca aacactgact ggccccaagc cacgcaagat gcccgccttc
     2941 gccagcaaat tgacagatac cgaaatcgcg cgcgtattga ctttcgtgcg caccacctgg
     3001 ggcaaccagg catcgcccgt attgacgcgc gacgtggcgg cgctgcgtgc ccggctcgaa
     3061 aagtgatgtt ctcgaaagac ctattcccgc ccttccactc ccactccata aacgtgcacc
     3121 atctgacctc caagccaacc cgatacgccg atcaggctta cgccgatcac tgacaaaata
     3181 ataggggttt tcatgcttgc agggctaccg gcacgtatcc atacattcac cagatacaac
     3241 accaccgcaa caaggtttat gaccatgtgg gtgactgcca acttcttgat gggtggcgtg
     3301 gcgcctttgt aatagatcag gtcaatcaat cccggcaccg cagcaagcag cgcgccaatc
     3361 acgccgccca ccatggtgta gaacgcgact gtgtgccaaa ccgtcggtgc ggcggcgcca
     3421 aggccaatca aatcgcaaat aaacgagaaa acccacaaac cgatcgggaa gacgaccagc
     3481 atgggatgga tggggtgctt ggcgatactg gcaggtgtgc gcattagatt tccttttcgt
     3541 ttttgggtaa gccagcaact accatgcctg cagatgcgcg ggtacgcaaa ttgctcccgg
     3601 tgattgccgt tggagtcgtc gtcggcgtga ccaaatccac gcgcaatgcg cacaggagca
     3661 agcatgaata agtcggaaat ccccacagca cagaagaacg ttttgagtct tttgcttgac
     3721 gatcatcgta aagtgaagaa gatcttcaat gacttcaaaa ctgagaaaaa cactgcgaag
     3781 aaacagagca tggtggccga ggcgtgcaca gagctaacgg tgcacactca aatcgaagaa
     3841 cagaagtttt atcctttttt gcgggatcag gaaccggcgg tttttggcga cttgctggac
     3901 gaagcgctcg ttgaacatgc cactgccaag gagctgatcc gccaactgcg cgaggcgggc
     3961 ccgcccgatg acttgtatga ggcaaaattt acggtactgg gagaatacgt aaatcaccat
     4021 gtcactgagg aggaaaatga actcttcccc aaggtcatct caaaaaaggt gaacctgcaa
     4081 gatctgcaac cggagattga gcaactcaaa aaagaactga tgtcaaaggc gtcgaaatct
     4141 tagggcgctc tcggctcaat gaggcttgct ttgcctcact aaacgtgatg acacgaggag
     4201 ttgatcatgc aagaagaaac agtcgccatg gccgcttacc ccgaagcgcg gcctccggcc
     4261 gatctctcgg attcaaatac ttccggcgta tcgtgggcag ccattttcgc tggcgctgct
     4321 gctgccgctg cgctttctct cattttggta atccttggct tcggtcttgg cctgtccgct
     4381 gtttcgccgt gggcttatgc gggagccggt gctttgacaa taggaattgc gacggctgtc
     4441 tggttggccg caatgcaaat tattgcatcg gggttgggcg ggtacattgc gggccggctg
     4501 cgcgtcaagt ggccaggcct tcatactgat gaagtctatt ttcgagacac ggcgcacggt
     4561 atgctgagct gggccatcgc tacgctggta accgcagcgt ttatgggatc cgcgcttacc
     4621 ggaatcgtga gcggcggcgc cgagttggcc ggcaacgttg ccg
//
LOCUS       DATCXU010000044         8425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1164932,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000044 DATCXU010000000
VERSION     DATCXU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164932"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VIR04_00915"
     CDS             <1..1240
                     /locus_tag="VIR04_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY9459339.1"
                     /translation="DCRRYWASYRADEIDDDEITGVNNQLVASVGTCSVMGTASTMAC
                     IAEALGIMLPGGATAPAVTADRLRVAERTGARAVALIGSGLTPEKILTAKAFENALRV
                     LLAIGGSTNGIIHLTAIAGRLGIKIDLNEFDKMSSETPVLVDLKPSGTHYMEDFHKAG
                     GLTTVLRELKPLLHLDALTITGRTLGQELEAAGPAFPQEVVHPFNRPVYGQGSVAVLR
                     GNIAPDGAIIKQSAASQDLMEHEGRAVVFESMADMARRLDDPDLDVRADDVLVLKQTG
                     PVGAPGMPEAGYIPIPKKLARQGVKDMVRMSDGRMSGTAAGTIVVHVTPESACGGPLR
                     LIRNGDRIRLSVANRSLDVLVDKTELDARAADMPLAEADPKVRGYRKLFLRSVTQADQ
                     GCDFDFLQAADFTQSVPRPK"
     gene            1276..2427
                     /locus_tag="VIR04_00920"
     CDS             1276..2427
                     /locus_tag="VIR04_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY9459340.1"
                     /translation="MQDKVDDYAKDSRIVSVQARTISIPLDNPTSFSTRKVSARDYTL
                     VRVKTADGCEGIGFCYGGSRGGSLVTHAVRELFNDMLTGQSSLRVEGLWQSMYQETLL
                     HGRAGSVMRALSILDVAMWDRNARAAGLPLSQYLGGFAKDTVPAYASGGYYLDGKTPE
                     MLGEELAGYVAKGFKAVKMKVGRFSPDEEEARIRAAREAIGDDVLLMLDANNAWHDLP
                     TALEYMKRYEPYDPYFIEEPFSPDDINNHAELGRRTSVPVATGEIEAGRWRHQELLEK
                     RAAMILQTDAAVCGGISEWRRIAAMAAGFGVAVYPHWFHDLHVHLVAATPNAAYVEFF
                     PDDQVLNFRRLVDTQLEFVNGKLIVPTRPGLGFGFDEKAVEKYALDKWA"
     gene            2499..3416
                     /locus_tag="VIR04_00925"
     CDS             2499..3416
                     /locus_tag="VIR04_00925"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY9459341.1"
                     /translation="MNAAKRVTGVFSPVVTPFTSDLQPDAARLAKHCQWLLSQDVGLA
                     VFGTNSEANSMSVAEKSKLLDALIKAGIPADRLMPGTGCCALTDTVELTRHALQHGVG
                     RVLMLPPFYYKGVSDDGLYASFSEVIQRVGDDRLRIYLYHIPQVSNVPITLNLIERLL
                     KAYPAAIAGIKDSSGDWNNTDAMLKQFQPQGFDVFAGSERFLLATLRGGGAGCITATG
                     NVNPGPIARLYKTWQQADADKQQQALSDTRSLFEKFSMIPALKAAVAHFAKDPDWAMV
                     RPPLVSLNNQQRAALATSLEAHGFSMPGL"
     gene            complement(3418..4347)
                     /locus_tag="VIR04_00930"
     CDS             complement(3418..4347)
                     /locus_tag="VIR04_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9459342.1"
                     /translation="MKRNLPPLSALRAFEAAARHLSFTKAGIELNVTQGAISRQIKIL
                     ESYLKTSLFNRLTRQVELTPFGQCYLTAISLALDAVEQATHDSTSTHHSLSISVLPSI
                     GSLWLMQRLTVFACAYPEIRLHISSSLDPADFKHDRIDLAIRHGKLPGQVYCDSSPQI
                     EFHMTEDWTGITAIHLWDDYVTPVCSKKLLEKYGPLECPQDLSRFCLIYNDRRADVWQ
                     SWLNANGAETITGAGKLEFTHSFLVANAARQGLGVACLSTVEVEPVDWRDEIVLPFKS
                     RVKSAGGYYLIAPQEKLQSPDAKLFLNWLAALL"
     gene            4477..5361
                     /locus_tag="VIR04_00935"
     CDS             4477..5361
                     /locus_tag="VIR04_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY9459343.1"
                     /translation="MNKLTQTHLYDFIPIIDRPPITLPHGARIAVIPYVNIEHFPENV
                     SGTALVPGTAGLTPDTLNYGWRDYGNRVGLWRMIEIMDAVGMRGTVCLNSDSIREYPR
                     IIEEGQKRGWAWMGHGLNNSPTSFLTGLEEAEERKIIGEVLTTMEQALGRKTKGWIGP
                     FLSETYNTPHILADLGVEYLCDFTADDQPFKIKTRTGSLISMPYTLELNDVPAVLNLG
                     QSGSAFGDMIIDQFDVLYEEGATIARIMPICLHTFLVGQPFRAKHLKRALQYIAGHSN
                     VWLTTGDEVNDWYRKNYL"
     gene            5375..5578
                     /locus_tag="VIR04_00940"
     CDS             5375..5578
                     /locus_tag="VIR04_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459344.1"
                     /translation="MENPVGLKPFRLMVDLRAAGWSEERIETARAVTVLIDETVERVA
                     YEKLAISDHIYSFGTWCEHSGGT"
     gene            5578..6993
                     /locus_tag="VIR04_00945"
     CDS             5578..6993
                     /locus_tag="VIR04_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY9459345.1"
                     /translation="MNDFEKLDLIGVADGIRRRRFSARDIALWSIERLETLGVRYNAV
                     FRIDGEAALARAGDLDDRQARGEPCGPLHGIPLAHKDLYGVRGRECHAGSLIFKGRIA
                     ERTAFALMGLDDAGQVNLGSLHMSEFALSPTGFNGHYGSGLNPWNPDYVSGGSSSGSG
                     IAVAARLIFGSLGSDTGGSIRLPAAMCGVTGLKTTFRRISAEGCVPAAFSLDSFGPIA
                     QSARDCARLLTHCSHPNAADGLSTSMAADDYETCLSGMLQGLRIGVPEQYFREDLDQE
                     VASCLDASLATFKACGAAIVSARIPDMSRINSLASLVMSVEAAAVHRRWIESRPMDYS
                     EQVRNRIEQGFAYSAVRYAEALSLRTPILAEFLGLAFAECDVLHVPVLPIVTPTIVAT
                     TQGTAQDILRVISRITHATRGINYLGLPAISVPAGLSKAGLPIAYQLIGRPFAEGVLL
                     RAADAFQRETDWHRQIPALRM"
     gene            7069..8292
                     /locus_tag="VIR04_00950"
     CDS             7069..8292
                     /locus_tag="VIR04_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459346.1"
                     /translation="MNLRSIIFVSVLNGALASVAAVPAQAAAFSDNAVNIGVLTDMSG
                     VYSDFSGTGSVTAVKMAVEDFGGKVNGHPVNVIFADHLNKADTGAALAREWYDRDKID
                     AIFDTSNSAVSLAVIGVTREKNRIVVIGGSSTTKITTDSCTPNSIQYVYDANALSNVV
                     AKALIDKGKKTWYFITVDFAFGHGLERTTTNVIKAAGGKVLGSVLHPLNTADFSSYVI
                     QAQASHADVIALATGGTDMVNAIKTANEFGVSASGKQSVASLLTFITDVHSMGLKQAQ
                     GLVLTEAFYWDLNDETRAWSKRFFEKTNKMPSMVHAGMYSATMHYLNAIKAVDTDAAS
                     TVMAKMKSTPVNDFFVKGGKIRADGLHVHDMYLFQVKTPSESKRPWDYYKLLATVPGE
                     KAFSPMAPGACKFVK"
     gene            complement(8309..>8425)
                     /locus_tag="VIR04_00955"
     CDS             complement(8309..>8425)
                     /locus_tag="VIR04_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyvinyl-carboxyphosphonate
                     phosphorylmutase"
                     /protein_id="HEY9459347.1"
                     /translation="SSGRLDEDPSLVAPFSERQRLVSKDFYDQLEQKYRTES"
BASE COUNT         1826 a         2163 c         2505 g         1931 t
ORIGIN      
        1 tgactgccgc cgttattggg ccagctaccg cgccgatgaa attgacgacg acgagattac
       61 cggcgtcaac aaccagcttg tggccagtgt cgggacctgc tcagtaatgg gcacggccag
      121 cacaatggcc tgtattgccg aagcgctggg gatcatgctg cctggcggag ccaccgcgcc
      181 cgcggtaacc gccgaccggt tgcgggtggc tgaacgcact ggcgcgcgcg ccgtggcgtt
      241 gattggttcg gggttgacac ccgaaaaaat tcttactgcc aaagcgtttg aaaacgcgtt
      301 gcgtgttttg ctggcgatag gcggatccac caacggcatc attcatttaa ccgcgatagc
      361 aggccggttg ggcatcaaaa tagacttgaa cgagttcgat aaaatgagtt ccgagacacc
      421 ggtgcttgtg gatctgaaac cgtcgggcac acattacatg gaagattttc acaaggcagg
      481 cggcttgacc acggtcttgc gcgaattaaa gccgctcttg catctggatg cgctcactat
      541 tacggggcga acgctgggcc aggaactgga agccgcgggt ccggcattcc cgcaagaggt
      601 tgtccatccc ttcaatcgcc ctgtttatgg ccagggtagt gttgcggtgt tgcgcgggaa
      661 tattgcgccc gacggagcga ttatcaaaca gtccgccgct tctcaggatc ttatggaaca
      721 cgaaggacgc gcggttgtgt tcgaaagcat ggcggatatg gccaggcggc ttgacgatcc
      781 cgatctggat gtccgcgccg acgacgttct ggtattaaag cagaccggcc cggtgggggc
      841 ccccggcatg cccgaggcgg gctacatacc catacccaaa aaattggccc ggcaaggggt
      901 caaggacatg gtgcgcatgt ccgacggccg catgagcggc acagcggcgg gaactattgt
      961 tgttcatgtc acgccagagt ccgcgtgcgg cggcccattg cgcctgattc gaaacggcga
     1021 tcgcattcga ttgagcgtgg cgaaccggtc gcttgatgtt cttgtcgaca agacggaact
     1081 cgacgcgcgc gccgccgata tgccgctggc ggaggcagat cccaaggtgc ggggctatcg
     1141 caaattattt ttgcgttcgg tcacgcaggc cgatcagggc tgcgattttg attttcttca
     1201 ggcggccgat ttcacgcaaa gtgtgcccag gccgaaataa gccaacacca cgattcattt
     1261 ttcaggggat tacgcatgca ggacaaggtt gacgattacg ctaaagattc acgcatcgtg
     1321 tcggttcagg cccgcacgat cagcattccg ctagataacc caacgtcttt ttccacgcgc
     1381 aaagtatcgg cgcgcgatta caccctggtc agggtaaaga cggccgacgg ctgcgagggt
     1441 attgggtttt gttatggcgg cagtcgtggc ggctcactgg ttacgcacgc cgtgcgcgag
     1501 ctgttcaatg acatgctgac gggccaaagc tctttgcgcg tcgaaggcct gtggcagagc
     1561 atgtatcagg agactttgct gcatggccgc gcaggctctg tcatgcgcgc gttgtcgatt
     1621 cttgatgtgg ccatgtggga tcgcaatgcg cgtgccgcgg gcttgcctct gagtcaatat
     1681 ctgggcggtt tcgcgaaaga cactgtgccc gcatacgcca gcggcggtta ttaccttgat
     1741 gggaaaacgc ccgaaatgct gggcgaggag ttggccggct acgtggcgaa gggattcaag
     1801 gccgtgaaaa tgaaagtcgg ccgtttcagc cccgacgaag aagaggcgcg catacgcgct
     1861 gcacgcgagg cgattggcga cgatgttttg ctgatgctcg acgccaataa cgcgtggcac
     1921 gatttaccca cagcgcttga atacatgaag cgttatgaac cctacgatcc ctatttcatt
     1981 gaagagccat tcagcccgga cgatatcaat aatcacgccg aactgggacg gcgcacgtcg
     2041 gtgccggtgg cgacgggcga gatcgaagcg ggccgttggc gccaccagga attgctggaa
     2101 aagcgggccg caatgatatt gcaaacggac gcggccgtat gtggcgggat aagcgaatgg
     2161 cgccgcattg ccgcgatggc cgcgggtttt ggcgtggcgg tttatcctca ctggttccat
     2221 gacctgcatg ttcatctggt ggcggctacg ccgaacgcgg cctacgtcga attcttcccc
     2281 gatgatcaag tgctgaattt cagacgtctg gtggacactc aactggagtt cgtgaatggc
     2341 aaattgattg tgccgacccg gccgggtctg ggctttggct tcgatgaaaa agcggttgag
     2401 aaatatgcgc tggataagtg ggcgtagcgt tggccgcggc tggcgcggcg aatgcgcgct
     2461 tgaatctaca ctaatgtaaa gacttaaagg agattgaatt gaacgcagca aaacgagtaa
     2521 ccggcgtgtt ctcgcctgtg gtgaccccct ttacttccga tttgcagccc gatgcggctc
     2581 gtttggcaaa gcattgccag tggctgttaa gtcaggatgt cggcctggcc gtttttggca
     2641 cgaattccga agccaactcg atgtcggtgg ctgaaaaaag caagctgctc gatgccttga
     2701 tcaaggcggg catacctgcc gatcgcctga tgccggggac gggctgctgc gctttaacag
     2761 acacagtgga attgactcgg catgcgctgc aacacggggt gggccgcgtg ttgatgctgc
     2821 cgccctttta ctacaagggc gtgtcggacg acggtctgta tgccagcttt tcggaagtca
     2881 tccagcgggt gggcgatgat cggcttcgaa tttatttgta ccacattccg caagtgtcca
     2941 atgtgcccat tactcttaat ttgatagagc gtttgcttaa ggcctatccc gctgccatcg
     3001 caggaattaa agacagttcg ggcgactgga ataataccga cgcaatgctc aagcagtttc
     3061 agccgcaagg ctttgacgtc tttgccggca gcgagcgctt cttgctggcg actttgcgag
     3121 gcggtggggc gggttgcatc accgccaccg gcaatgtgaa tccgggtcca atcgccaggc
     3181 tgtataaaac ctggcagcaa gccgacgccg acaagcaaca acaggcctta agcgacacac
     3241 gcagcctgtt cgagaagttt tccatgatcc ccgcgttgaa ggcggctgtt gcgcactttg
     3301 ccaaggatcc cgactgggcc atggtgcgcc caccattggt aagcctgaac aatcagcaaa
     3361 gggccgcctt ggccacttcc ctggaagcgc acggcttttc gatgccgggt ttgtgagcta
     3421 aagcagggcg gcaagccagt taaggaaaag cttcgcgtcg ggtgattgga gcttttcctg
     3481 aggcgcaatc aggtagtacc ccccggcgct ctttacccgg cttttgaatg ggagaacaat
     3541 ttcgtcgcgc cagtcgaccg gctcgacttc gactgtcgaa aggcaagcaa cgcccagccc
     3601 ctggcgcgcc gcattggcaa ccagaaatga atgggtaaac tccagcttgc cggctcccgt
     3661 tattgtttca gcgccattcg cgtttaacca gctttgccag acgtccgctc gacggtcgtt
     3721 ataaatcagg caaaaacggg agaggtcttg cgggcactcg agcggcccat atttttcgag
     3781 cagcttttta ctgcacacgg gtgtcacgta gtcatcccat agatgaattg cggtgatgcc
     3841 cgtccagtct tcagtcatgt ggaattcaat ttgcggcgag gagtcgcaat agacctggcc
     3901 cggcaacttg ccgtgacgga tagccaggtc tatccgatca tgcttgaaat cagccggatc
     3961 cagggagctt gatatgtgca ggcgaatctc gggataggca cacgcgaaga cggtcaggcg
     4021 ctgcattaac cacaggctgc caatagatgg caagacgctg atggacaggg agtgatgcgt
     4081 gctggtggaa tcgtgagtcg cctgttcaac ggcgtccagt gccagcgaaa tggccgttag
     4141 gtaacactgc ccgaatgggg tcagttcgac ctggcgcgtc aatcggttga ataacgaggt
     4201 tttgagataa ctttccagga tttttatctg gcggctgata gcgccctgcg ttacgttcag
     4261 ctcgataccc gccttggtga agctaaggtg ccgcgccgcc gcctcgaatg ccctcagtgc
     4321 cgatagagga gggagatttc ttttcattct cacctatcat ttttactgat ccatgccgga
     4381 aaataaatcg tttgtttacg cgtcctatcg ctagcattat aggagcgctg gtttccgttg
     4441 cggcttgata ttaacgttac cgggtgaaac gagatcatga ataaattgac ccaaactcat
     4501 ctttacgact tcatcccaat catagaccgg ccgcctatta ctctgccgca cggcgcgcgt
     4561 attgccgtaa ttccgtacgt caatatcgag cactttccag agaatgtatc gggaaccgct
     4621 ctggtgcccg gtacggcagg gttgacaccc gatacgctga attacggctg gcgcgactat
     4681 ggcaatcgtg taggcctgtg gcgaatgatc gaaatcatgg atgcggtggg gatgcgaggt
     4741 acggtctgtc tgaattctga ttccatacgg gagtatccgc gaatcattga agagggtcag
     4801 aagcgaggct gggcctggat gggccacggt ctgaacaatt ccccgaccag ttttctgact
     4861 ggcctggaag aagcggaaga gcgcaagatc atcggcgagg tcttgactac gatggaacag
     4921 gccctgggaa ggaaaaccaa agggtggatc ggcccttttc taagtgaaac ctacaacacg
     4981 ccgcacatac ttgcggactt gggagttgaa tacctttgcg atttcacggc tgacgatcag
     5041 ccattcaaga ttaaaaccag gacgggcagc ctgatatcaa tgccctacac gctggaactg
     5101 aacgacgtgc cggcagtctt gaatcttggg caatccggga gcgcatttgg ggacatgatc
     5161 attgatcaat tcgatgtgct gtacgaagaa ggggcgacta ttgcccgaat aatgcctatc
     5221 tgcctgcata cttttctggt tgggcagcca tttcgcgcca agcacttgaa gcgcgctctt
     5281 caatatattg ccggccactc caatgtgtgg ctgacgacgg gcgatgaagt taacgactgg
     5341 tatcgcaaga actatctctg accgggcgaa gattatggag aatcccgttg gcttgaaacc
     5401 attccggctt atggtcgacc tccgtgcggc aggctggtct gaagaacgca tcgagacggc
     5461 tcgggcggtc accgtgttga tagatgaaac ggtcgaacgt gtcgcatatg aaaaactcgc
     5521 tatcagcgat catatttata gtttcgggac gtggtgcgag cattcaggcg gcacgtaatg
     5581 aatgatttcg aaaaacttga cttgattggt gtggctgacg ggattcgtcg tcgccggttc
     5641 agtgcgcggg atatcgcact gtggtccatt gaaaggcttg agactctggg ggtacgctat
     5701 aacgcggtat ttcgcattga cggcgaagcc gcactggctc gagccgggga tcttgatgat
     5761 cggcaagcgc gtggcgaacc ctgcggtccg ttacatggca ttcctcttgc gcacaaggac
     5821 ctttacggag ttcgcgggcg cgagtgccac gccgggtcgc ttatttttaa aggacgaatc
     5881 gctgagcgca cggcttttgc gcttatgggt ctggatgatg cgggccaggt caatcttgga
     5941 tccttgcata tgtccgaatt tgctttgagt ccgacagggt tcaatggtca ttacggctcg
     6001 gggcttaacc catggaatcc ggattatgta agcggtgggt catccagcgg atccggtatt
     6061 gcagtcgctg caagattaat attcggctca ttgggcagcg atacaggcgg ctcgattcgt
     6121 ctgccggcgg caatgtgcgg cgtcactggg ctcaagacaa ccttcaggcg cattagcgcc
     6181 gaaggttgtg ttcctgccgc tttttccctg gacagcttcg ggcccatagc ccaaagcgct
     6241 agagattgcg ccagactgct tactcattgc agccacccta atgcggcgga tgggttaagc
     6301 acaagcatgg cggctgacga ttacgagact tgtttgagcg gcatgttgca gggcttgcgc
     6361 attggcgtgc ctgaacaata ctttcgggag gatctcgacc aggaggtcgc gtcgtgcctg
     6421 gatgcaagtc tggccacttt caaagcttgc ggagctgcca ttgtgtcagc tcgcatccca
     6481 gacatgtccc gcattaattc tctggcttcg ctggttatgt cggtggaagc ggctgcggtg
     6541 catcgcagat ggatagagtc caggccgatg gattattccg aacaggtgcg taatcgcatc
     6601 gagcagggct ttgcctattc cgccgttcgt tatgcggaag cgctgtcgct gcgcacccca
     6661 attttggctg aatttctcgg attggctttc gctgaatgtg atgtgttgca cgtgccagtt
     6721 ttgcccatcg tcacgccgac tattgtcgca acgacgcagg gtacggcgca ggacattcta
     6781 cgagtgatca gccgtataac ccatgccacg cgcggcatca actatcttgg ccttcccgcg
     6841 atcagcgtcc ctgcgggatt atcgaaggcc ggcttgccga tagcgtatca attaataggg
     6901 cgtccctttg ccgaaggcgt tttgcttagg gccgcggacg ctttccagag agaaacggat
     6961 tggcaccgtc aaattccggc gcttcgcatg tgagcggcgc atcggatcgt tgacgcgttg
     7021 acgcggcgct caacgaagtt agttgcaacg gcaaaatgga gaagcatcat gaatttacgt
     7081 tctattattt ttgtcagcgt tttgaatggc gcccttgcct cggtcgcagc ggttccggca
     7141 caggcggccg ccttttcgga taatgcggtc aatatcgggg tactcacgga catgtcgggt
     7201 gtgtactcgg atttttcggg taccggctcc gtcacggcag taaaaatggc ggtagaggat
     7261 ttcggaggca aggttaatgg ccacccggtc aacgtcattt ttgccgacca tttgaataaa
     7321 gccgacacag gcgccgcgct cgctcgcgaa tggtacgacc gtgacaagat cgatgccatt
     7381 ttcgacacgt cgaactcggc cgtgtcgctg gcggtgattg gagtgactcg tgaaaagaat
     7441 cgcatcgttg tcattggcgg gtcgagcacg acgaaaatca ccaccgactc gtgtacgccc
     7501 aacagtattc aatatgtgta tgacgccaat gcgttgtcga atgtcgtggc caaagcgtta
     7561 atcgacaagg ggaagaaaac ctggtatttc attaccgtcg attttgcttt cgggcatggg
     7621 ttggaaagga cgacgacaaa tgtgatcaag gcggcgggag gaaaggtgct gggttcggtg
     7681 ttgcaccctt tgaatacagc ggacttctca tcgtatgtta ttcaggcgca ggcttcgcat
     7741 gccgacgtca ttgccctggc cacgggcggc acggacatgg tcaatgccat taagaccgcg
     7801 aatgaatttg gcgtttcggc gtccggcaag caaagtgttg cttcattatt aaccttcatc
     7861 accgatgttc acagtatggg attgaagcag gctcagggcc ttgttctgac ggaagcgttt
     7921 tactgggatt tgaacgacga aacccgcgca tggtcgaagc gattctttga aaagaccaat
     7981 aagatgccca gtatggttca cgcgggcatg tattcggcga cgatgcatta tcttaatgcc
     8041 ataaaggcgg tcgacaccga tgccgcttcg acagtcatgg caaaaatgaa atcaacgccg
     8101 gtcaatgatt tctttgtgaa aggaggcaag attcgcgcgg acggcctgca tgtgcatgac
     8161 atgtatctct tccaggttaa aactccgtca gaatccaaac gcccctggga ttattacaag
     8221 cttctggcga ctgtgcccgg ggaaaaggcg ttctcgccga tggcccccgg agcatgcaag
     8281 ttcgtcaaat gaaaaaaggg tcgtaaattc aggattccgt gcggtatttt tgttcgagct
     8341 ggtcgtaaaa atctttgctg accagtcgct gtctttcgga gaagggcgcc accagactgg
     8401 ggtcctcgtc aagacggccg cttga
//
LOCUS       DATCXU010000045         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_541315,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000045 DATCXU010000000
VERSION     DATCXU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541315"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VIR04_00960"
     CDS             <1..1462
                     /locus_tag="VIR04_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEY9459348.1"
                     /translation="VLREHSGNVLIIAQPWRVELSLTLAVLLTLACFISLYIVLRVFA
                     WIASGPERFRSWRGLRAENRDHQLLENGWINVLEGRYNQAEKELSKVLGRSNSGSRKV
                     LAALALARAAHHLGEFTRRDEALDLARDNAGDDPRLKEATATVAAEMCLDQNRAQEAL
                     ALLQPLQDASSRYFNATRLLLQAHRQLNNHDRVYELTRLLARRGVVEKPEALRLIETS
                     AAARLRAGGPDRFKAIWGDLKSDERVLPEVALAAASVQESSGDFDEASKILEAAIGYR
                     LEPRLLAAYSQCPPDQVARRLSKAEVWLKSYPDDPALLASLGNLCLTGQLWGQGERYL
                     LRSEEIRSDVRIHALLGNLYDRLGRQADAMKHWRLASSVAGVLPALPISHELPAADMR
                     GDPTLIDIESPPEPGFVDSDQAAPVADAADSAANAPAGQALAKAADAAQSPPRDSAPA
                     YTSPDSRLDEYFDSAPIPGVDLSQTSDQPRDGSNRR"
     gene            1490..2026
                     /gene="ppa"
                     /locus_tag="VIR04_00965"
     CDS             1490..2026
                     /gene="ppa"
                     /locus_tag="VIR04_00965"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY9459349.1"
                     /translation="MSLDRVPAGAKLPDEFNVIVEIPMNSAPVKYEVDKKTSAIFVDR
                     FLLSAMHYPCNYGYIPRTISNDGDPVDVLVISPFPIQIGAVIRCRSIGMLEMDDEAGG
                     DAKLLALPIDKLYPPYRKIKAASDLPDEDLARIHHFFEHYKDLETGKWVKVKNWLGLD
                     AAHKEIMDGVARYNKSLA"
     gene            2101..2796
                     /locus_tag="VIR04_00970"
     CDS             2101..2796
                     /locus_tag="VIR04_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY9459350.1"
                     /translation="MQYIVPVQPVAALAVAGTDQQFPVRRIYCVGRNYAKHAREMGFT
                     GREDPFFFSKPADAILNVADGQTGTLPYPPKTSNLHYEIELVVALDKGGRDLSPEQAA
                     DCVWGYAIGLDMTRRDLQGEAKKAGRPWEVGKAFDHSAPIGPLHPRSKVGTLSTGAIS
                     LAVNGTKKQDGELSDMIWSVAESVAYLSGLFELKAGDIIFTGTPDGVGPVVAGDAMVG
                     AVNGLGELHVAVK"
     gene            2863..2994
                     /locus_tag="VIR04_00975"
     CDS             2863..2994
                     /locus_tag="VIR04_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY9459351.1"
                     /translation="MRLKVLISLAIMGCMALAGCAHTVAGAGEDITNAGHAISKTAK"
BASE COUNT          709 a          914 c          968 g          752 t
ORIGIN      
        1 ggtcttgcgc gaacatagcg gcaacgtact tattatcgcg cagccatggc gggtggaatt
       61 gtcgctgacc ttggcggttt tactgactct agcctgcttt ataagcctgt atattgtttt
      121 gcgcgttttc gcgtggatcg cgtccggccc cgagcgtttt cgttcctggc gcggcttgcg
      181 ggctgaaaac cgtgaccatc aattgctgga aaatggatgg attaacgtgc tcgaaggacg
      241 ctacaaccag gccgagaagg aactttcgaa agtgctcggg cgaagcaatt ccggctcccg
      301 caaggttttg gcggcattgg cattagccag ggcggcgcat catttggggg aattcacgcg
      361 gcgcgatgag gcgcttgacc tggctcgcga caatgccggc gatgacccgc gtctgaaaga
      421 agccaccgcg accgtagcgg ccgaaatgtg tctggatcag aaccgcgccc aagaggcgct
      481 ggcattgttg cagcccttgc aagacgccag ttcgcgttac ttcaatgcca ctcgcttact
      541 gttgcaggcg catcgtcaac tgaacaatca cgaccgtgtc tacgagctta cgcgtttgct
      601 ggcgcggcgc ggcgtcgtgg aaaagcccga agccctgcgt cttattgaaa cgtcggccgc
      661 ggctcgtttg cgcgctggcg ggcccgatcg cttcaaggcc atttggggcg acctgaagtc
      721 tgacgagcgg gttctgcccg aagtggcgct ggcggccgcc tctgtgcagg agtcaagcgg
      781 cgattttgac gaagcctcga aaattctcga ggctgcgatc ggctaccggc ttgagcctcg
      841 tttgcttgcc gcttattctc agtgcccgcc ggatcaggtg gccaggcgtt tgagcaaagc
      901 cgaagtctgg cttaaatctt atcctgacga ccccgcattg ttggcctctt taggcaattt
      961 gtgccttacc gggcagcttt ggggccaggg cgaacggtac ttgctgcgta gcgaggaaat
     1021 ccgcagcgat gtgcgcatcc acgctttgct cggaaattta tacgaccgcc tgggaaggca
     1081 ggccgacgcc atgaaacact ggcgcctggc atccagcgtc gcgggggtgt tgcctgcatt
     1141 gcccatcagt catgagcttc cggccgccga tatgcgcggg gatcccacac tgatcgatat
     1201 cgaaagcccg cccgagcctg gatttgtaga ttccgatcag gctgcaccgg tggcggatgc
     1261 ggcggattct gccgccaacg ccccagccgg gcaggcgctt gcgaaagctg ccgatgcggc
     1321 gcaatcgccg ccgcgcgatt cggccccggc ttatacttcg cccgattccc gcctggacga
     1381 atactttgat agcgcaccca ttccaggcgt cgatttatcg caaacgtcgg atcaaccccg
     1441 cgatggctcg aatcgccgct aattctttaa acatttacaa ggctagaaca tgagtttaga
     1501 tcgagtgccg gccggcgcta aactgcccga tgagttcaac gttatcgtcg agatccccat
     1561 gaactccgct cccgtcaaat acgaggtcga taaaaagacc agcgctatat ttgtcgatcg
     1621 ctttctgctt tccgcaatgc attacccctg caattatggg tacataccca gaacgatttc
     1681 caatgatggc gatcccgtcg atgtgctggt tatttctccg ttccctattc aaattggcgc
     1741 tgtcattcgc tgccgctcga tcggcatgct ggaaatggac gacgaagcgg gcggcgacgc
     1801 aaagttattg gctttgccca tcgacaagtt gtatcctcct taccggaaaa taaaggcggc
     1861 ttctgatttg cccgatgaag atctggcgcg tattcaccac tttttcgaac actacaaaga
     1921 tctggaaacc ggcaagtggg ttaaagtcaa aaattggctt ggacttgacg ccgcccataa
     1981 agagattatg gatggcgttg cccgctacaa taaatcactg gcatgacatc gtctccacgg
     2041 atatagttca tcgcccgatc atcaaccagt tcatttggct tatttcccga ggaagaaaaa
     2101 atgcaatata ttgttccggt tcagcctgta gccgctcttg ccgtggcggg gaccgaccag
     2161 cagtttcctg tgcgccgcat ttattgtgtt ggacgcaact atgccaagca tgccagggag
     2221 atgggtttta ccggccgcga agatccgttt tttttcagca aaccggccga cgccatattg
     2281 aacgttgccg acgggcaaac cggaacattg ccgtatccgc ctaaaacatc gaacctgcat
     2341 tacgagattg aattggtggt cgcgctggac aagggcggcc gtgatttgtc gcctgaacag
     2401 gctgctgatt gtgtttgggg ctacgcaata ggacttgata tgacgcggcg tgaccttcag
     2461 ggcgaagcca agaaggcggg acgcccttgg gaagtcggca aggctttcga tcattctgcg
     2521 ccgattgggc cattgcatcc ccgcagtaaa gtgggcacat taagcaccgg cgccatttcg
     2581 ctggcggtta acggtacaaa aaaacaggac ggcgaactct cggatatgat ctggagcgtg
     2641 gctgaatcgg tcgcctacct ttccggcttg ttcgagctta aggcggggga catcatcttc
     2701 accggcacac ccgatggcgt gggcccggta gttgctggtg acgccatggt gggcgccgtc
     2761 aatggcctgg gtgaacttca cgttgctgtg aaatgacagc aaatttttcg gtttagcact
     2821 atagaatccg cctgttaagg gctattagcg aaaggagcga ccatgcgttt aaaagtcttg
     2881 atatcgttgg ctattatggg ttgtatggcg ctggccggtt gcgcgcatac ggtggcgggc
     2941 gcgggcgaag acatcaccaa cgcgggtcac gcgattagca aaaccgcaaa ataaggtaac
     3001 cttctgcagg aagaaaaaag ccccggccgg ggcttttttc attgaagcaa ggcgcagctt
     3061 tgcccagctc ggaatttccg aattgtggtc cggccggcgc agcctcacac tgggaccctg
     3121 aaagctaaat caggagctca cggtgcgaca ctcgtacttt actcaacgcg gccagggtat
     3181 gacggaatac atcatcgtcg ttgccttggt ggcggtcgcc gctattgcgg tctaccaatt
     3241 gtttggccag gtcgtgcgct cacaaacggc ggccatggca aaagagctgg ccggcgaaga
     3301 cggatccgaa cagtcacaca tggcgcaatc agccgccgag gcc
//
LOCUS       DATCXU010000046         8304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_462243,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000046 DATCXU010000000
VERSION     DATCXU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462243"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VIR04_00980"
     CDS             complement(<1..1319)
                     /locus_tag="VIR04_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY9459352.1"
                     /translation="MNGKKLSGEAELENLQEQSFSYFLHEASLTNGLVMDKNAPGWPA
                     SIAATGLALAAYPVAVERGLMTYSWALERTLMVLRFFWASPQGPEPDATGYKGLYYHF
                     LDMRTGRRAWQCELSTVDSAFLLAGALTAAIYFSAASPDETEVRTLADALYQRADWEW
                     ALNGGQTLTHGWKPESGFIRYRWEGYDEALLLYILALGSPQHAIPESSYSAWLSTYRW
                     ESCYGYEYVYAGPLFTHQLSHCWVDFRGIQDAYMREKGIDYFENSRRATYVQQQYAIE
                     NPLRFVGYGECCWGITASDGPGPATLKIEGLQRRFMDYEGRGVPYGPDDGTIAPWAVV
                     ASLPFAPDIVLPAIDHYVNNLKLSRNNRYGFKATYNPTYVRKKGKRPVADDDRPRGWV
                     SSWHFGINQGPIVLMIENYRTGLLWELMRRCPYIVQGLRRADFSGGW"
     gene            complement(1316..3040)
                     /gene="pgm"
                     /locus_tag="VIR04_00985"
     CDS             complement(1316..3040)
                     /gene="pgm"
                     /locus_tag="VIR04_00985"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY9459353.1"
                     /translation="MSKSKEAMKISPLAGKPAPLSLLINVPRLITCYYAETPDVTVPV
                     QRVAFGTSGHRGSAFENTFNEWHVLAITQAICDYRKRHDINGPLFIGIDTHPLSVPAS
                     ATALEVFAANGVLAMISQDDETTPTPAVSHAILTYNRGRKTGLADGVVITPSHNPPDN
                     GGYKYNATNGGPADVAVTGWIEKRANALLENSVKDVRRWTYKRALYADTTHRHDFAGA
                     YVNDLGNILDMEAIRAAKLHMGTDPLGGAGVHYWGRIAERYNLNLTVVSDEVDPTFRF
                     MSVDWDGKIRMDPSSPYAMQRLIKLKDRFDIAFGCDTDYDRHGIVAKSVGLLAPNHYL
                     AVAIDYLFTHRSQWDSGAAVGKTVVSSQIIDRVARKLGRKLIEVPVGFKWFVDGLFDG
                     SLGFGGEESAGASFLRKDGSVWTTDKDGMIAALLSAEMTAVTGRDPGESYKALAHELG
                     DPVSERFEAAATPQQKKRLAKLSAHQIRSTDLAGEKIVSVLTNAPGNDAPIGGVRVSA
                     QSGWFAARPSGTENIYKIYAESFQGKEHLQRILTQAQAMVDAALEVEPHAAVKMAQEP
                     SSVNKGSV"
     gene            complement(3050..5119)
                     /gene="tkt"
                     /locus_tag="VIR04_00990"
     CDS             complement(3050..5119)
                     /gene="tkt"
                     /locus_tag="VIR04_00990"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY9459354.1"
                     /translation="MTDHAQLDQLCINTLRTLSIDAVQKANSGHPGTPMGAAPAAYCL
                     WQRFLRYDPDHPDWPNRDRFVLSAGHACALLYSMLYLCDVKATRPDREPPGPEAITMD
                     DLKHFRQAGSRCSGHPEYGLASGVETTTGPLGQGVATSVGMAIAGRWLAATYNRPDYE
                     LFDYNVYALCSDGDMMEGISSEAASLAGHLQLANLCWIYDSNHISIEGSTNLAFTEDV
                     ATRFASYRWNVLHVHDANDLEALTHAYDTFLNTTDRPTMIIVHSHIGYGAPHKHDTRE
                     AHGSPLGEEEVRLAKQFYGCDPDLQFDVPDGVQAHFRKHLGERGAAGFAQWTAQADAY
                     RAQYPDLAVALDCIWERQLPSGWDESLPVFPADAKGLATRVSSGKVLNAIAAKVPWLI
                     GGAADLAPSTMTDLTAPLVTSFQPPGPEGNYQGRNFHFGIREHAMCAIASGMSVSGVR
                     PYASSFFVFTDYCRGAVRISALMEIPVIYIWTHDSISLGEDGPTHQPVEQLAGLRAMP
                     GMIVIRPADANEVVAAWRVIMRTTDCPVSLVLTRQNVPTFDRDQYASAAGLEQGAYVM
                     ADAPDKKPDVLLLATGSEVSLCIAAYEQLKTEGINARVVSMPCWELFEAQSQEYRDSV
                     LPPSVTARVAVEEASPFGWERFTGFHGAILGMRTFGLSAPGKVASEHFGFTFENVVKA
                     ARAQLTR"
     gene            complement(5116..6072)
                     /locus_tag="VIR04_00995"
     CDS             complement(5116..6072)
                     /locus_tag="VIR04_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEY9459355.1"
                     /translation="MKRGYTIPLYLLPFDHRNSYVKGMFNFSGSLSTEQRDKVADSKQ
                     VIYEGFQQAVGNGISTGRGGLLVDEEFGALILRDAAQKGYITALSTEKSGSDEFEFEY
                     GDEFASHIEQFDPTFAKALVRYNTEGDDALNRRQDERLRRLSDYCQNTGRLFMFELLV
                     PATSVQMERVKGNKETYDLDFRPELMARAIAHLQDQGIEPDVWKIEGLDRRSDCEHIV
                     ETVRRGGRVDASCIVLGRGADELKVAHWLTVAATVPGFIGYAVGRTTFWDAIADYEAG
                     RATRQQASARIAARYGDWARTFEQNRPSRNSSAARSDTEVRS"
     gene            complement(6069..7757)
                     /gene="pgi"
                     /locus_tag="VIR04_01000"
     CDS             complement(6069..7757)
                     /gene="pgi"
                     /locus_tag="VIR04_01000"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY9459356.1"
                     /translation="MTTPASPGALSGSAAVLPGLALTDLPAWKALQSHYQATMRGTHL
                     RELFDQDPLRGEHFAAEAAGVYLDYSKNRITSQTISLLMSLAEQRGLRQHIEAMFAGQ
                     EINTSERRPVLHVALRAPASENIFVDGQNVVPGVHAVLERMAQFSAMVRSGQWLGYTG
                     KRIKNVINVGIGGSALGPVMAYNALQYYSQRDMTFRFVSNMDPTDFVEATRDLAPEET
                     LFIICSKTFGTLETLTNAHAARRWCLDKLGNDEALKRHFVAVSTNEEKVSQFGIDTAN
                     MFGFWDWVGGRYSIDSAIGLSTMIAIGPSQFTEMLAGFHDMDEHFRHAPLRENLPALM
                     GLLSVWNTNFFDARTVAVLPYAEYLTRFPAYLQQLAMESNGKHVTLDGDRVDYQTGPI
                     YWGEPGTNGQHSFYQLIHQGTHFIPCDFIGFCQSLNPLTNQHDELMSNLFAQAEALAF
                     GKTYAQVVAEGIPSWQAPHRVFDGNRPSNVILLKRLTPRALGSLIALYEHSVFTQGAI
                     WNIDSFDQWGVELGKALARRTSPELESVGEPELSHDSSTNTLIRRYRRLNKEQS"
     gene            complement(7754..>8304)
                     /locus_tag="VIR04_01005"
     CDS             complement(7754..>8304)
                     /locus_tag="VIR04_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY9459357.1"
                     /translation="LAADLDPHVRSVASLFVSRWDVAVKDKVGPSLQNRLGIAIAQST
                     YQAYRELLASPRWRTLADAGAAPQRLLWASTGTKDPKAPDTLYVQALAAPDTINTIPD
                     KTLLAFADHGQVKELLANDGGDCEAVLDRFKKEGIDQDALAARLQTEGTRSFDKSWNE
                     LLERIASKSEKLKKSKTAGAKQ"
BASE COUNT         1832 a         2455 c         2213 g         1804 t
ORIGIN      
        1 aaccacccac cactgaagtc ggcgcgccgc aagccttgga caatatatgg gcaacgcctc
       61 atcagctccc acaacaggcc cgtcctgtag ttttcaatca tcaacacgat cggcccctga
      121 ttaattccga agtgccacga cgatacccat ccacgcggcc tgtcgtcgtc cgccacaggc
      181 cgctttccct tcttccggac ataagtggga ttgtaagtgg ctttaaaacc gtaccggtta
      241 ttgcgcgaca atttaagatt gttaacataa tgatcgatcg cgggtaaaac gatatcgggc
      301 gcgaacggca acgatgccac gacggcccag ggggcgatcg tgccgtcatc agggccatag
      361 ggaacgccgc gcccttcata atccatgaac cggcgctgaa gtccttctat cttgagcgtg
      421 gccggacccg ggccgtcgct ggcggtgatt ccccaacagc actcgccata tcccacgaat
      481 cgcaatgggt tctcgatcgc gtactgttgc tggacatacg tcgcgcgccg gctattttca
      541 aaatagtcga tgcccttttc gcgcatgtag gcgtcctgga tgccgcgaaa atcgacccag
      601 caatgagaca attgatgcgt aaacaggggg cccgcgtaga cgtactcgta accgtagcaa
      661 gattcccagc ggtacgtgct caaccatgcc gaatagctgc tttcgggtat ggcatgctgg
      721 ggggatccaa gcgccagaat ataaagcaaa agcgcctcat cgtagccttc ccagcggtag
      781 cggataaatc cggactcggg cttccagcca tgcgtcagtg tttggccgcc attaagagcc
      841 cactcccaat cagcgcgctg gtacaacgca tcggcaagag tgcgcacctc ggtttcatca
      901 ggcgaggcag cgctgaaata gatagccgcc gtcagggcgc cggccaatag aaaggcgcta
      961 tcgaccgtcg acaattcaca ctgccaggcg cgccgcccgg tgcgcatatc aagaaaatga
     1021 taataaagac ctttgtagcc agtggcgtca ggttccgggc cttgcgggct tgcccaaaaa
     1081 aagcgcaaca ccatcaaggt gcgttcgagc gcccaggaat aagtcattag cccgcgttca
     1141 acggccaccg gatatgccgc caacgccagg ccggtcgcgg cgatgctggc cggccagccg
     1201 ggcgcgtttt tatccatgac cagcccatta gtcaaacttg cttcatgcaa aaaatagctg
     1261 aatgattgct cttgcaagtt ttcaagctcg gcttctccgc ttaatttctt gccgttcaca
     1321 ctgatccttt gttgaccgaa gagggctcct gcgccatttt taccgctgcg tgcggttcga
     1381 cctcgagcgc cgcgtcgacc atagcctgag cttgtgtcaa aatccgttgc aagtgctcct
     1441 ttccttgaaa gctctctgcg tatattttgt aaatattttc agttcctgac ggccgggcgg
     1501 caaaccagcc gctttgcgca cttactcgca cccctccgat gggcgcgtcg ttgcccggcg
     1561 cattggtcaa aacggagacg attttttccc ccgccagatc agttgaccgg atctgatgcg
     1621 ccgacagctt tgccaatcgc tttttctgtt gcggcgtggc ggcggcctcg aaccgctcgg
     1681 aaaccggatc gcccagctcg tgcgccaacg ctttataaga ttcacccgga tcacgccccg
     1741 taaccgcggt catctcggcg gaaagcaaag cggcgatcat gccgtccttg tcggtcgtcc
     1801 agaccgagcc gtccttgcgc aggaacgagg cccccgcact ttcttcgccg ccaaaaccca
     1861 gtgaaccatc gaacaatcca tccacaaacc acttgaaacc gactggcact tcgatcaatt
     1921 tacgtcccag ttttcttgct acacggtcaa tgatctggct gctgacgacc gttttcccca
     1981 cagccgcgcc agaatcccat tgactgcggt gcgtaaataa ataatcgatc gcgacggcaa
     2041 gataatgatt cggggcaagc aagccgacgc ttttggcgac gatgccgtgc cgatcgtaat
     2101 cggtatcgca tccaaacgcg atatcgaagc gatccttcaa tttgatcaac cgttgcattg
     2161 cgtaaggtga ggacgggtcc atgcgaattt ttccgtccca atccacggac atgaagcgaa
     2221 atgtcggatc gacctcgtcg cttactacgg tcaaattaag gttatagcgc tctgctatgc
     2281 gaccccagta atgaacgccg gcgccgccca gcggatcagt tcccatgtgg agtttcgccg
     2341 cgcgaatagc ctccatatcc aggatattcc ccagatcatt tacgtatgcg cccgcgaaat
     2401 cgtgccgatg cgtggtgtcg gcatacaaag cccgtttata cgtccagcgc cgcacatcct
     2461 tgacactgtt ctcaagcaag gcgttggccc gtttttcaat ccatcctgtc acggcgacat
     2521 ccgccggccc gccattggtc gcattgtatt tataaccacc gttatcgggc ggattgtgcg
     2581 atggcgtaat gacaacccca tcggccaatc cggttttacg tccgcggtta taggtcaaaa
     2641 tcgcatgcga gacagccggt gtcggcgttg tttcatcatc ttgtgaaatc atggccagca
     2701 cgccattggc ggcgaatacc tcgagcgcag tggccgatgc cggcacagat aaaggatgcg
     2761 tgtcaatgcc gataaacaag ggaccgttga tgtcgtgccg tttgcgataa tcgcagatgg
     2821 cttgtgttat ggcgagaaca tgccactcgt taaacgtgtt ctcaaatgcc gaaccccgat
     2881 gaccagacgt tccaaacgcg acgcgttgaa cgggaacggt cacgtccggt gtttcggcgt
     2941 aatagcacgt gatcagccgt gggacgttta tcagcagcga caacggcgcc ggtttgcccg
     3001 ccaacgggct gattttcatg gcttctttac tcttgctcat tgtgccgcct tatcttgtca
     3061 actgagcgcg tgccgccttc acgacgtttt cgaaagtaaa accaaaatgt tcggaggcca
     3121 ctttgcccgg cgccgacagc ccaaatgtgc gcatgcccag tatcgcccca tgaaaaccgg
     3181 taaagcgttc ccatccaaat ggagaggctt cttcgaccgc gacgcgggcc gtcactgagg
     3241 gcggcaacac actgtctcga tattcctggc tttgcgcctc gaacaattcc caacatggca
     3301 tgctgacaac acgagcgttt atcccctcag ttttcaactg ttcataagcg gcaatgcata
     3361 aagagacctc gcttccggta gccagcaata gtacatccgg ctttttatcg ggcgcatcag
     3421 ccatcacata agccccctgc tccaggcccg cggcggatgc atactggtcg cgatcgaacg
     3481 ttggcacatt ctggcgcgtc agcaccagag acaccgggca atccgtggtc cgcataatga
     3541 cacgccaggc ggccaccact tcgttcgcat cggctgggcg aatcactatc atgcccggca
     3601 tcgcccgcag tccggccagt tgttcaactg gctgatgcgt gggtccatct tcgcccaggc
     3661 tgatcgaatc gtgcgtccag atataaatga ctggaatttc catcaaggcg ctgatgcgca
     3721 ccgccccgcg gcagtaatct gtgaatacga aaaagctcga cgcatacgga cgcactcccg
     3781 acacgctcat gccgctggct atcgcgcaca tcgcatgttc gcgtatgccg aaatggaaat
     3841 tgcggccttg atagttgcct tctggtccgg gcggctgaaa actggtcacc agcggcgctg
     3901 tcagatcggt cattgtcgag ggcgccaaat cggcagctcc gcctatcaac catggcactt
     3961 tggccgcaat ggcattaagc actttgcccg acgatacccg ggtcgccaaa ccctttgcat
     4021 ctgccggaaa taccggcaag gattcgtccc agcccgatgg caattgacgt tcccaaatgc
     4081 aatccagcgc aaccgcaaga tcgggatact gagcgcgata cgcatcggcc tgcgccgtcc
     4141 attgcgcaaa cccggccgcg ccgcgctcgc ccaaatgctt gcgaaagtgc gcctgcacgc
     4201 catccggaac atcgaactgc agatccggat cacagccata aaattgcttg gccagacgaa
     4261 cctcttcctc gcccaaagga gacccgtgcg cttcgcgggt atcgtgttta tgaggcgcgc
     4321 cgtagccgat gtggctatgc acgataatca ttgtgggacg gtcggtcgta ttcaaaaagg
     4381 tgtcatacgc gtgcgtaagc gcttcaagat cattggcgtc gtgcacgtgc agcacgttcc
     4441 agcgataact ggcaaaacga gtcgcgacat cctcggtgaa agcaagattc gtcgaccctt
     4501 cgatggaaat atgattggaa tcgtaaatcc agcaaaggtt ggccagttga aggtggccgg
     4561 ccaaggacgc cgcttcgctg cttatgccct ccatcatgtc gccatcgctg cacagcgcgt
     4621 atacgttgta atcgaatagc tcgtaatcgg gacggttata ggttgccgcc agccaacgcc
     4681 cggctatcgc catgccgacg ctggtcgcga cgccctgccc caacggcccc gtcgttgtct
     4741 cgacgcccga agcgagcccg tattcaggat gtcccgaaca ccggctgccg gcttggcgaa
     4801 agtgcttcag atcgtccatg gttatagctt ccgggcccgg aggctcgcga tccgggcgcg
     4861 ttgccttcac atcgcacaaa tacagcatgc tgtagagcag cgcgcaggca tgaccggccg
     4921 atagcacgaa gcggtcgcga ttgggccaat caggatgatc gggatcgtag cgtaaaaacc
     4981 gctgccacaa acaatacgcg gccggcgcgg cgcccattgg agtgcccggg tgacctgaat
     5041 tagccttttg caccgcgtcg atggaaagcg tgcgcagcgt attgatgcaa agctgatcaa
     5101 gctgtgcgtg atcagtcatg atctcacctc ggtatcggaa cgcgccgcac tgctattgcg
     5161 cgacgggcgg ttctgctcga aagttcgggc ccagtcgccg tagcgtgcag cgatgcgcgc
     5221 gctggcctgt tgccgcgttg ccctgcccgc ctcgtagtcg gcgatggcat cccagaaggt
     5281 cgtacggccg accgcatacc ctatgaaacc cggcacggtc gccgcaacgg tcagccagtg
     5341 agccactttg agttcatcgg cgcctcgccc cagcacgatg caactggcgt cgacgcgacc
     5401 gccgcgtcga accgtttcaa caatgtgctc gcaatcagac cggcgatcca ggccctcgat
     5461 cttccagaca tcaggctcaa ttccctgatc ttgcaaatgg gcaatcgccc gcgccatcaa
     5521 ttcgggacga aaatcaagat cgtaggtttc tttgtttccc ttgacgcgtt ccatctgtac
     5581 cgaagtggcg ggcaccagca actcgaacat gaacaatcgc ccggtgttct ggcaataatc
     5641 agaaagtcgt ctgagccgtt catcctggcg gcgattaagc gcgtcgtctc cttcggtgtt
     5701 gtagcgcacc agagccttgg caaaggtcgg gtcgaattgt tcgatatggc tggcaaattc
     5761 atcgccgtat tcgaattcga actcgtcgga accacttttt tccgttgaca gggccgtgat
     5821 gtatcctttt tgggccgcat cgcgcagaat cagtgcgccg aattcctcat cgaccagaag
     5881 tccgcctcgg cccgttgaaa ttccattgcc cacggcctgt tgaaatcctt cgtaaataac
     5941 ctgcttgctg tcggccacct tatcgcgctg ctcggttgaa agagatccgg agaaattaaa
     6001 catgcccttg acgtaagaat tcctgtggtc gaaagggaga agataaagag gaatggtgta
     6061 accccgtttc atgattgctc cttgttaagt cgacggtaac gccggatcag tgtgttggtg
     6121 gagctatcgt ggctcagctc aggctcaccg acactttcca gttcgggcga cgtgcgccgc
     6181 gccagcgcct tgcccagttc gacaccccat tgatcgaatg aatctatatt ccagatagcg
     6241 ccctgggtaa acacgctgtg ttcataaagg gcaatcaggg agcccaacgc acggggagtc
     6301 agacgcttta ataaaatgac gttgctcgga cgatttccat caaatacgcg gtgcggcgcc
     6361 tgccacgatg gtatgccttc ggcaacaact tgcgcgtaag tcttcccgaa ggccaacgcc
     6421 tcggcctgag cgaataaatt cgacatcagt tcgtcgtgct ggtttgtcaa cggattcaat
     6481 gactggcaaa atccgatgaa atcacacgga ataaaatgcg taccctggtg tatcaattga
     6541 taaaacgaat gctggccatt cgtgcccggc tcgccccaat aaatcgggcc cgtctggtaa
     6601 tccacccgat cgccatcaag cgtgacgtgt ttgccattgc tttccatggc gagctgttgc
     6661 agataagcgg gaaaacgcgt caggtattcg gcgtaaggca aaacggccac ggtgcgcgca
     6721 tcgaaaaaat tggtgttcca gacagacagc aagcccatca aagccggcaa attctcgcgt
     6781 aacggtgcat gacgaaaatg ctcatccatg tcgtggaaac ccgccagcat ctcggtgaac
     6841 tggctggggc cgatggcgat cattgtggac aatccgatgg ccgaatcaat cgaatagcgg
     6901 ccgccgaccc aatcccagaa gccaaacata tttgcagtat caataccgaa ctgcgacacc
     6961 ttttcctcat tcgtcgaaac tgcgacaaag tgccttttca gtgcctcgtc attgcccaat
     7021 ttatccaggc accagcgtcg cgccgcgtgg gcgttggtca gggtttcaag cgttccaaac
     7081 gtttttgagc aaatgatgaa caacgtctct tcgggggcaa ggtcgcgtgt cgcttcgaca
     7141 aaatcggtgg ggtccatgtt cgaaacaaag cgaaaagtca tatcgcgctg actatagtat
     7201 tgcagcgcgt tgtaagccat gactggcccc agtgcggagc cgcctatgcc aacattaatg
     7261 acgtttttaa tgcgtttgcc tgtgtatccc agccattggc cgctgcgcac catcgctgaa
     7321 aactgcgcca ttcgttcaag cactgcatgt acgcccggca ccacattttg accatcgaca
     7381 aaaatgtttt cgctggccgg agctcgcaat gcaacatgca atacaggacg gcgctcggaa
     7441 gtgttgattt cctgtcctgc gaacatggct tcgatgtgtt gacgcagccc gcgctgctcg
     7501 gccagactca tcagcaacga aattgtctgg ctggtgattc tgttttttga ataatcgaga
     7561 taaacacctg ccgcttcggc ggcaaaatgt tcgccacgca gcggatcctg atcgaacagc
     7621 tcgcgcaaat gtgtaccgcg cattgtcgcc tgataatggc tctgaagcgc cttccatgca
     7681 gggagatccg tcagtgccag tcccgggagg acggctgccg agccggagag agcacccggc
     7741 gatgcagggg tcgtcattgc ttggctcccg ctgtcttgct ctttttcagc ttctcgcttt
     7801 tagaggcgat gcgttccagc agctcattcc aggatttgtc gaaagaccgg gtgccttcgg
     7861 tctgtagccg ggccgccaaa gcgtcttgat caataccctc ttttttgaac cggtcaagca
     7921 ctgcctcgca atcgccgcca tcgttggcaa gcaattcttt cacctgaccg tgatcggcaa
     7981 aagccaacag agttttatcg ggaatcgtgt taatcgtgtc gggagcggca agtgcctgta
     8041 cgtaaagcgt gtcgggcgcc ttggggtctt tggtgccggt gctggcccac agcaatcttt
     8101 gcggcgcggc tcccgcatcg gccaatgtgc gccaacgtgg cgatgccagc agttcgcgat
     8161 acgcctggta cgtgctttgt gcaatcgcaa tacccaggcg gttctgcaag ctgggcccaa
     8221 ccttgtcttt cacagccacg tcccaacggc tgacaaaaag cgaggcaacg gaacgcacat
     8281 gagggtccag atcggcggcc agcc
//
LOCUS       DATCXU010000047         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_64124,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000047 DATCXU010000000
VERSION     DATCXU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64124"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VIR04_01010"
     CDS             <1..1850
                     /locus_tag="VIR04_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9459358.1"
                     /translation="IISISTSLIAVFIPLLLMGGIVGRLFREFAFTVTLTILMSVIVS
                     LTLTPMLCSRFLKNYEHETHGRVYRAFERAFDMMKNGYQRGLIVVLRHQFSTLCVFLA
                     TVVATIVLFIIIPKGFFPQQDTGYIYGFAESAQNSSFGSMKERVLALAEVIQQDPDVT
                     TFGVNSGQTTFNTGTFFISLKPKDQGRTASADQIIARLRPKLAHIQGINLFMQAGQDI
                     NVGGRLSRTQYQYTLTDANLDELNEWAPKLLEKFRSLPELTDLASDQQNAANTATLTI
                     DRARASSFGISPAQIDATIYDAIGQRQVAQYFTQINSYNVILEVTPELQQDPNLFSKL
                     YLTSPLTGKQIPLSTLVTIDTNKTGYLSISHQSQFPAVTISFNLAPGVALGQAVTAIN
                     KAQASMNLPQAMGGSFQGTAKAFGESLATQPYLIAAALIAVYIVLGFLYESYIHPLTI
                     LSTLPSAGVGALLILMLNGYDLSVIALIGIILLIGIVKKNGIMMVDFALSAERDLGMS
                     PKDAIYQACLLRFRPIMMTTACALFSGLPLMLSQGAGAELRRPLGFAMVGGLLLSQAL
                     TLFTTPVIYLYLDRAHYWFLRRKKARLARKLRKMRKAAQGGLPASNNAE"
     gene            1885..2571
                     /locus_tag="VIR04_01015"
     CDS             1885..2571
                     /locus_tag="VIR04_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY9459359.1"
                     /translation="MKVLIVEDETKTADYLHKGLTEQGCAVDLAHTGADGQHLALHVD
                     YDVIVLDVMLPGVDGFSVLRNVRSVKQTPVIMLTARDGVEDRIKGLHEGADDYLVKPF
                     SFLELLARLQALTRRGRTQEPVRLHIGDLLIDLISRKAYRGAVRIDLTAKEFALLAVL
                     ARRQGEILSKTSIAELVWDMNFDSNTNVVEVAIKRLRAKMDTPFNPRLLHTIRGMGYV
                     LELRDQERAE"
BASE COUNT          587 a          743 c          697 g          581 t
ORIGIN      
        1 ccatcatttc aatttctact tcgctgattg cggtattcat acccttattg ctgatgggcg
       61 gaatcgtggg ccggctgttt cgcgaattcg ccttcacggt tacgctgaca attctgatgt
      121 cagtcatcgt ttccctgaca ctgacaccca tgctgtgttc gcgctttctt aaaaattacg
      181 agcacgaaac ccacggtcgt gtgtacaggg cgtttgaacg cgcctttgac atgatgaaaa
      241 atggctatca acgcggtttg attgtggtgt tgcgccatca attcagcacg ctgtgcgtat
      301 tccttgcaac cgtggtggcc accatcgtgt tgttcattat tattccgaag gggttttttc
      361 cccagcaaga caccggttat atatacggtt ttgcggaatc ggcccagaac tcctcattcg
      421 gatcgatgaa agaacgggtg ctcgccctgg ccgaggttat ccagcaagat cccgacgtga
      481 cgacattcgg tgtaaacagc ggacaaacca ccttcaacac cggcacgttc ttcatcagcc
      541 tgaaacccaa ggaccagggt cgcacggcca gcgcggacca gattatcgcc cgcttgcgcc
      601 ccaagctggc gcatatccag gggatcaact tgttcatgca ggccggacaa gacatcaacg
      661 ttggcgggcg tttgtcgcgc acccaatatc aatatacgct caccgatgcg aacctggatg
      721 aactgaacga atgggcccca aaactgctgg aaaaattccg cagcctgccc gaactgaccg
      781 acctggcatc cgatcaacaa aacgcggcca ataccgcaac cctgactatt gatcgcgcgc
      841 gggcatcaag ctttggcatt tcgcccgccc agatcgacgc aaccatttac gatgcgatag
      901 gtcagcgtca ggtcgcccag tacttcacgc aaatcaatag ttacaacgtc attctcgagg
      961 tcacccctga gctccagcag gatccgaacc tgttcagcaa gctttatctg acatcgcccc
     1021 tgactggcaa gcaaatcccg ctatcaactc ttgtcaccat cgacaccaac aaaacgggct
     1081 atctgtccat cagtcatcaa agtcaatttc cggcggtaac gatttcgttt aatctggcgc
     1141 ccggcgtggc tttggggcag gcagtcacag ccatcaataa agcgcaggcc tcgatgaatt
     1201 tgccacaagc catgggcggg tctttccagg gcacggccaa ggcatttggc gaatcgctcg
     1261 cgacgcagcc ctacctgatc gcggcggcgc tgatagccgt ctatatcgtt ctcggctttc
     1321 tatatgaaag ttacattcac ccgctcacga ttctgtcgac attgccatcc gccggcgtgg
     1381 gcgcgctcct gatactgatg ctcaacggtt atgatctgag cgtcatcgcg ctgatcggca
     1441 ttattctgct gatcgggatc gtcaaaaaga acggcatcat gatggtggac tttgcgttat
     1501 cggccgagcg agatctgggg atgtcgccga aagatgccat ttaccaagcc tgcctgctgc
     1561 gctttcgccc catcatgatg accaccgcct gcgccctgtt cagcggcctg cctttaatgc
     1621 tcagccaggg cgccggcgcc gaactcagac gcccgctggg gttcgccatg gtcggaggtc
     1681 ttcttttatc gcaggcgtta acgctgttca cgacgccggt tatctatctg tatctggacc
     1741 gcgctcatta ctggttcctg cgacgcaaaa aagcgcgact ggcgaggaaa ttaagaaaaa
     1801 tgcgcaaagc ggcgcaaggc ggactgccgg cgtcaaacaa cgcagaatga tctaaactac
     1861 gacggagcgg aaacggtgtt tccaatgaaa gtactgatcg tcgaagatga aacgaaaacc
     1921 gccgattact tgcataaagg gttgacggaa caaggctgtg ctgtcgatct ggcgcacacc
     1981 ggagcagacg ggcagcattt ggctctgcat gttgactacg atgtcatcgt gctcgacgtc
     2041 atgctcccgg gggtggatgg tttttcagtg ctgcgcaacg tgcgatcagt caagcaaacg
     2101 cccgtcatta tgctcaccgc gcgcgatggc gtagaagacc ggatcaaagg cctgcacgag
     2161 ggggccgacg attatctggt caagccattt tcgttcctgg agctgcttgc ccgcctgcaa
     2221 gccctgacac ggcgcggacg cacgcaagaa cccgtgcgat tgcatattgg cgatttgctt
     2281 atcgacctga tcagtcgcaa ggcatatcgg ggcgccgtcc gaatcgacct gactgccaaa
     2341 gaattcgcgc tactggctgt gctggcgcgg cgccagggcg aaattctgtc caaaacctcc
     2401 atcgccgaac tggtgtggga catgaatttc gacagcaata cgaatgttgt cgaggtagcc
     2461 atcaaacggt tgcgggccaa gatggacacg ccttttaatc cgcggctgct gcataccata
     2521 cgcggcatgg ggtacgttct ggagctgcgc gatcaggagc gcgctgaatg aggcgctcga
     2581 tcacgatgcg gctggtggcc atgttcgc
//
LOCUS       DATCXU010000048         6871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1083630,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000048 DATCXU010000000
VERSION     DATCXU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083630"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /gene="nhaA"
                     /locus_tag="VIR04_01020"
     CDS             <1..1209
                     /gene="nhaA"
                     /locus_tag="VIR04_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY9459360.1"
                     /translation="VILTNSPWGPGFAAFWSQEFGLVFGAADFRLTVLQWVNDGLLSL
                     FFLTVGLEVKREFTVGRLSNRKSAAFPIAAAIGGMVVPALIYRLILPGGPWAHGWGVP
                     LSTDTAFAVALIVMMGSRVPIELRIFLTAAAIVDDIGAIIVVALFYSDDLHIAYLFAS
                     LAATILLALLNRWNVYRAAPYAVTAVVLWAFVHASGLHATLAAVILAFFVPTRPPANL
                     RALTAQADAIIRLEAENNNEGLGRRLSTPALNALDAIHDRLESPAGRVLRHIEPWSTY
                     AVLPVFALANSGLVITPGIWGEHGPLMAAIMLGLILGKPVGMVLASALAVRWGVATKP
                     DAYSWWQVLGAGALAGIGFTMSLFIAGEAFPDASDFAAAKIAIFTASLIAAVLGLAIL
                     WKAGNTKEAN"
     gene            complement(1258..2481)
                     /locus_tag="VIR04_01025"
     CDS             complement(1258..2481)
                     /locus_tag="VIR04_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY9459361.1"
                     /translation="MPSTWHDLTDTIWDVVIIGAGPAGAATAITLTRLGQRVLLVDEL
                     ISNRFKLGESLPPTSIGLVKHFLGNLEGPEQHLPGFFRTAGNVSLWATDQAEVTDFFF
                     TLTGFGLCVERSTFDEALRSNAVAAGATLLKGVRFESCARIPDCPFNWQLTLTSATQT
                     RQYGARYLVDCSGRRAAVARALGVTTVHNDDCLFAYAQWYSCLGDDDDRYTRIEAAPH
                     GWWYSNRLPGAEGGKTSRLVVFHSDKDLPAARMAARSQGFGELLGDSTHIAPLLKARD
                     YHPCGTIRGAPANSQRIRDFCGDAWMAVGDAAQAYDPLSSQGIDKALKTGSHAGHMIH
                     YALTNCPEGAAGLGSHNAYIHQYDEQQHQLWQTYLSHRDFYYRAQPRWSDQPFWQRRR
                     RLNDGSTFPRPAPPT"
     gene            complement(2485..4485)
                     /locus_tag="VIR04_01030"
     CDS             complement(2485..4485)
                     /locus_tag="VIR04_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HEY9459362.1"
                     /translation="MSEKIKNIRIHPGIGIARMGDSDEFYIGPEAPGVVVDPGGNDGP
                     GPDGGSYRDSAARLKRQAQRYRVYAYDANDKVIAELTSDSGLVQSVSWRVHVRNMKAA
                     NYAFQGAYLFDPDELRNPSIQPGKKPIERNQLIIDPGVHTIASGQAGPVVMKGDVFTG
                     IEKGSLPGELRFEGFTPKDRSKEVEVTYKAARDIELGQLRLDSKDRLLFVPSAGKGEC
                     VTTPKVALSNPSETIAPPNGPENGKNPLTNQFAYFNVPGWWDDTCGGEIDVTVTLKDG
                     TVLSTRDNVKSVTDEGTRNPKAGAWIVTAPPKFAPHMYHVVSILDRVYEAFPEAYPYT
                     GQKTNFYRDIYPVFVNAVNYGWVSPFAAGVAPETKNIAHGPGQPGDLLTPGYMSAFTD
                     PLDKSKPIRQMIYGLMRHAPGKSARLVDSLLPAPPQRPTSWKNEEFQRAEEDRKMPKL
                     WGTGGKPLQNQQLGNSLPDQFLSLTDWQLNHLKNWADGNFEVGVRQKPVSLEQLPLTD
                     QPHALDSSALEPTIGGGFHPGIEFPYLIVYREYFAEAFRVGKDIEPGSLAAYMSSPWH
                     GDFWSCNVAWWPTQRPDIVFEYDKESQTRTYKEWFRGYDANGEPLSSTDGYNQMPYAW
                     PKLGMVLPIKTEDGSFLKDKGTTVFVERERNTALNRPPAKGT"
     gene            complement(4524..4811)
                     /locus_tag="VIR04_01035"
     CDS             complement(4524..4811)
                     /locus_tag="VIR04_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY9459363.1"
                     /translation="MGIKRRDFIKGCAAVWVSLLPGITRAANNQNANSALVPGADPHI
                     EKSIKAGFGGGFSVRNHTQSNGLTYADIEHFGNRYAVVSADLLDWKIVGTL"
     gene            5427..6638
                     /locus_tag="VIR04_01040"
     CDS             5427..6638
                     /locus_tag="VIR04_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459364.1"
                     /translation="MRSSILSRLLWIRALRAFADGYVSLLLPLYLLALGMGPLQVGIL
                     ATGALLGSGVLTLLVGLYAQEFKYRALLTMAALLMAGTGFSFAVVSDFWPLLLIAVVG
                     TLNPSSGDVSVFVPLEHAVLSGAVPTRQRTALFARYSLIGSLAGASGALFAGAADLGA
                     AVTSLDMQVVLRTMFVLYGVLGLVAAMMYATLPKELDLGKQQVPSAPLRESKRIVYTL
                     AALFSLDAFGGGFVVQSMVALWLFEKFQLTISTAGTIFFWTGILSALSYLAASRIAYR
                     FGLVNTMVFTHLPSNIFLILVPLMPTLAWAVLMLLARSALSQMDVPTRSSYVMGIVPT
                     NERAAAASVTSVPRSLASAASPFFAGYLLSVSTFGWSLVIAGALKIVYDLLLLRIFGK
                     LRPPEELDIEH"
     gene            6729..6871
                     /locus_tag="VIR04_01045"
                     /pseudo
     CDS             6729..6871
                     /locus_tag="VIR04_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004551139.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
BASE COUNT         1308 a         1861 c         2014 g         1688 t
ORIGIN      
        1 gtaattctga ccaactcgcc atgggggccc ggcttcgccg cattctggtc acaggaattt
       61 ggtcttgtct tcggtgcagc cgactttcgg ttgactgtat tgcaatgggt gaatgatggc
      121 ctgttgagct tgttctttct gaccgtaggc ctcgaggtta aacgcgaatt tacggtcggg
      181 cgtctttcca atcgaaaatc cgcggcgttt cctatcgcgg ctgcgatcgg cggcatggtg
      241 gtcccggcgt tgatatatag actgatcctt cctggcggcc cctgggctca tggatgggga
      301 gtgccccttt caacggacac agccttcgcc gtggcattga tcgtcatgat gggcagtcgt
      361 gtacccattg agctacgcat cttcctgacg gcggcagcca ttgtcgatga tattggggcg
      421 attattgtgg tcgcgttgtt ctattctgac gatctgcata ttgcttatct tttcgcttcc
      481 ctggccgcaa ctattttgct ggcgttgctg aatcgctgga acgtgtacag ggctgctccg
      541 tacgcagtga cagcagtggt tctgtgggct ttcgttcacg ccagcgggct tcatgccacg
      601 ctggctgccg taattctggc gtttttcgtc cctacccggc ccccggccaa tttgcgggct
      661 ttgacggcgc aggccgacgc tatcattcgg ctcgaggccg aaaacaacaa tgaaggatta
      721 gggcgcaggc tatccacccc agcactcaat gcgcttgacg ctatccacga tcgccttgag
      781 tcaccggccg gccgtgtctt gcgccatatt gagccatggt caacatacgc tgtcttgcca
      841 gtctttgctt tggccaattc cggccttgtc attacgcccg gaatatgggg cgaacatggg
      901 cctttgatgg cggcgataat gctgggcctg atattgggaa aacccgttgg gatggttctt
      961 gcgtccgcgt tggcggtgcg gtggggcgtt gcgaccaagc cagatgcgta ttcatggtgg
     1021 caagttctag gcgccggcgc gctagcggga atcggcttta cgatgtcgct gttcattgct
     1081 ggcgaagcct ttccagatgc atcagatttt gccgcggcca aaatcgccat atttacagct
     1141 tccctgatag cggcggtttt ggggttagcc attctgtgga aagcgggtaa cacgaaagaa
     1201 gcaaattgac gtgcattggc accagccacc gaagggttta ccggcctatg ttgcccttca
     1261 tgttggcggg gccggtctgg gaaaagtgct gccatcgttc aggcgccgcc gacgttgcca
     1321 gaacggctga tcagaccagc ggggctgcgc gcggtagtag aaatcccgat gcgacaagta
     1381 cgtttgccaa agctgatgct gttgctcgtc gtattgatgg atgtacgcat tgtggctgcc
     1441 caatcccgcc gcgccttccg ggcagtttgt cagcgcgtaa tggatcatgt gcccagcgtg
     1501 gctgcccgtc ttgagcgcct tgtcgatgcc ttgcgacgac agcgggtcat aggcttgcgc
     1561 ggcgtcgccc accgccatcc aggcatcacc gcagaagtct cgtattcgtt ggctgttggc
     1621 cggcgcaccg cggatagtcc cgcaagggtg atagtccctg gctttgagca atggcgcgat
     1681 atgcgttgag tcgcccagca attccccgaa accctgcgaa cgcgcggcca ttctcgccgc
     1741 cggcaggtcc ttgtcggagt gaaaaaccac caatcgactg gttttcccgc cttccgcgcc
     1801 tggcaagcgg ttgctatacc accagccgtg gggcgcggcc tcgatccggg tgtagcgatc
     1861 atcatcatcg ccgaggcagg aataccattg cgcgtaggca aacaagcagt cgtcgttgtg
     1921 gacggtcgtg acgccgagcg ctctggccac ggccgcccgt cgaccggaac agtcgaccag
     1981 ataacgtgcg ccatactgcc gggtctgcgt tgccgaagta agagtcagtt gccaattgaa
     2041 aggacagtca gggatacgtg cgcaggattc gaagcggacg cccttgagca aagttgcacc
     2101 ggctgcgacc gcatttgacc gcagggcctc atcgaaagtc gatcgttcga cacacaggcc
     2161 gaagccggtc agcgtgaaga aaaagtcggt gacttcggcc tggtcggtcg cccatagcga
     2221 aacgttgcca gccgttctga aaaagcctgg aagatgctgt tctggacctt cgaggttccc
     2281 caggaagtgc ttcaccagac cgatagaggt cggtggcagc gattcgccca gcttgaaacg
     2341 gttggatatc agctcatcca ccagcagcac gcgctggccg agcctagtca gcgtgattgc
     2401 ggtggctgcc cccgccggcc cggcaccgat aataacaacg tcccaaattg tatcggtcag
     2461 gtcatgccag gtcgacggca cgaattatgt tcctttggcg ggtggccggt tcagtgccgt
     2521 attgcgctct cgttcgacga acacggtcgt gcctttgtct ttcaaaaagc tgccgtcctc
     2581 agtcttgatt gggagcacca tccccagttt tggccacgca tatggcatct ggttgtaacc
     2641 atcggtggac gatagtggtt cgccgttggc atcgtatccg cggaaccact ctttataggt
     2701 tcgagtctgg ctctctttat cgtattcgaa aacgatatct ggccgttgtg tcggccacca
     2761 tgccacgtta cacgaccaga agtcgccgtg ccatgggctt gacatatagg cagcgagcga
     2821 gccaggttca atgtcttttc caacgcgaaa tgcctcggcg aaatactctc gatagacgat
     2881 caggtaggga aattctatac ccgggtgaaa gccaccaccg atggtcggtt cgagcgccga
     2941 actatcgagc gcatgcggct gatcggttaa cggcagttgc tccagggata cgggtttctg
     3001 ccggacaccg acctcaaagt ttccgtctgc ccagtttttc aaatggttca actgccagtc
     3061 ggtcaggctt aaaaattgat ccgggaggct gttgcccagt tgctgattct gcaagggctt
     3121 gccccccgtg ccccacagct tgggcatctt gcggtcttct tcggcgcgct ggaactcttc
     3181 gttcttccaa ctggttggcc gctgcggcgg ggccggcagc aatgaatcga ccaaccgcgc
     3241 actctttccg ggagcgtgcc gcatcaggcc atatatcatc tgtcttattg gcttgctttt
     3301 gtcgagtgga tcggtgaatg ccgacatata cccgggagtg agcaagtcgc ccggttgtcc
     3361 cgggccatgg gcgatgttct ttgtttcggg cgcgacgccc gccgcaaagg ggcttaccca
     3421 accgtagttg actgcattga cgaagacggg atagatatcg cggtagaaat tcgtcttctg
     3481 cccggtgtag gggtaagcct cgggaaaagc ctcgtagacg cgatccaaaa tggacaccac
     3541 atggtacatg tggggtgcga attttggtgg tgcggtgacg atccatgctc ctgccttcgg
     3601 attcctcgtg ccttcgtccg ttaccgattt cacgttgtcg cgtgtactta agacagtgcc
     3661 gtccttaagc gtgaccgtga cgtcgatttc accaccgcag gtatcgtccc accagcctgg
     3721 aacgttgaaa taggcgaatt ggttcgtcag tggattttta ccgttctccg ggccgttcgg
     3781 aggggcgatt gtttcactcg ggttcgacaa cgccaccttg ggtgtcgtga cgcattcgcc
     3841 ttttccagcc gacggaacga acagcagacg atccttggaa tcgaggcgaa gctgccccag
     3901 ctcgatgtcc ctggctgctt tataagtgac ctcaacttct ttcgaacgat ctttcggagt
     3961 gaaaccctcg aaacgcaatt cgccgggcaa tgaacctttt tcaatgccag tgaaaacatc
     4021 acctttcatg accacgggtc cggcctgccc ggaggcgatc gtatgcacgc cgggatcgat
     4081 aattagttga ttacgttcga tcggtttttt gccgggctgt atggacgggt ttcgcaactc
     4141 gtccgggtca aacagatagg cgccctgaaa tgcataattt gccgctttca tattcctgac
     4201 atgaacccgc caactcacgg attggaccag acccgaatcc gacgtaagct cggcgatgac
     4261 cttgtcgttg gcatcgtagg catacacccg gtaccgctgc gcctgacgtt tcaatcgtgc
     4321 cgcgctgtcg cggtagcttc caccgtccgg ccccgggcca tcgttaccac cgggatcgac
     4381 gacgaccccg ggcgcttcgg ggccgatgta aaactcgtca ctgtcgccca tgcgggcaat
     4441 gccaataccg gggtgaatgc gaatgttctt gatcttttcg gacacggctc atttccttta
     4501 caggtatgcg aatcctggat tgcttagaga gtgcccacga ttttccagtc cagcaggtcg
     4561 gcagacacga ccgcgtaccg attgccgaaa tgctcgatgt cggcgtaggt gagtccgttt
     4621 gattgggtgt gatttcgtac cgagaagccg ccgccgaagc cggccttgat cgatttttct
     4681 atatgaggat cggctcctgg gacgagtgcg ctgtttgcgt tctgattgtt cgcggcgcgg
     4741 gttatgcccg gcagcaacga aacccagacc gctgcgcacc ccttgatgaa atctctacgc
     4801 ttgatgccca tggcccatcg ctccctgatt tcaacccgtc ttgattcaaa ttatcataca
     4861 gattacttcg tcatagaaca agaactctat agtcgcagct tcgagcagta gcgcgatcgg
     4921 aggttaaatc tgggtctctt ggcggatcag gccgggccga ccgagaaagg aaggcgtgat
     4981 cagattgcat atctaatcat gcgatgtggc taggacagag tgatgtttgc tgacgggttg
     5041 cccgtaacaa tttgcagaaa tgatcactgg gggcaagttc acttatcata ccgacgccgc
     5101 attgggtgcg ggcggttttg cggaacatcc caagcacgtg gattatctgc tgggtgacga
     5161 gtgtgccgac ggattcataa ggagcgaatc atgacatgca ctgctgaccg caatctcgca
     5221 taatatttca acacttggtg tgatggtggt gatcgatgcg ccggctggcc tttatcgcat
     5281 gtgaaggaaa tggcatgctc cttgtgatga atctggacac gcgccaggtt atttccagct
     5341 acgctggtgg caggggtgaa cgatcaaggg tgttccggat catatagccg attcaccagc
     5401 atccgaacgc aagcgatatt ctgacaatga ggtcatccat cctatcccgt ctgttgtgga
     5461 ttcgagcgtt gcgggccttc gctgatggct atgtcagttt gctattgccg ctttatctgc
     5521 tcgcactcgg gatgggccca cttcaggtag ggatactggc caccggcgcg ctgcttgggt
     5581 ctggagtttt aacgctgctg gtaggactgt acgctcagga attcaaatat cgtgcattgc
     5641 tgacgatggc tgcgctgctc atggcgggaa cggggtttag ttttgccgtg gtcagtgact
     5701 tttggccgtt gctgctgatc gcggtagtcg gcacgttgaa tccttccagc ggcgacgtca
     5761 gcgtgttcgt gccgcttgaa cacgccgtgc tttcgggtgc tgtgccaacg cgccagcgaa
     5821 cggcgctctt tgctcgctat agcttgatcg gatcgttggc cggcgcatcg ggcgcgctct
     5881 ttgcaggcgc tgccgacctc ggcgcggctg tgacaagtct ggacatgcag gtggtgctgc
     5941 ggaccatgtt tgtcctatac ggcgtcttgg ggctggttgc cgcgatgatg tacgccacat
     6001 tgccgaagga actcgattta ggcaagcaac aggtaccgtc ggccccgttg cgcgagtcga
     6061 aacgcatcgt ctacacgctc gcggcattat tcagcctcga tgcgttcggc ggaggcttcg
     6121 tcgtgcagtc catggtcgcc ttgtggttgt tcgagaagtt tcagcttacc atttcgaccg
     6181 ccggaacgat ctttttttgg actggaatat tgtcagcgct ctcatatctg gcagccagcc
     6241 gcattgccta tcggttcggg ctggtaaaca cgatggtgtt tacccatttg ccgtccaata
     6301 tttttctgat tctggttcca ttgatgccga ctctggcatg ggcagtactc atgctgctgg
     6361 cgcgcagcgc gctgtcccaa atggatgtgc cgacgcgaag ttcttatgtc atggggattg
     6421 tgccaaccaa cgagcgcgcc gccgcagcta gcgttacgtc cgtgccgcgc agtttggcct
     6481 ctgcagcgag tccctttttc gcagggtatt tgctgagtgt gtcaacgttc ggttggtctt
     6541 tggtgatcgc aggtgcgttg aaaatcgtct atgacttgct gttgctgcgc atattcggaa
     6601 agttacgccc acctgaagag cttgatatag agcattaaaa ccgcagtcgc ttctgttgcg
     6661 gtttaatttc gtagcgttac ggtaggcaag tgcttccgag agtctaatgg ataattttat
     6721 aataaaactt caaggtcacg gtcggacgtg aagcacccat gcatggtgtg ccggtcggtg
     6781 ccgtgatggg gatcaataca tgggcggcct ttgccggctt ggatgatcgg gcggtgggtc
     6841 gacggcgatt ttgcggtaca ggagcaagag t
//
LOCUS       DATCXU010000049         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_616925,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000049 DATCXU010000000
VERSION     DATCXU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616925"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /gene="rpsF"
                     /locus_tag="VIR04_01050"
     CDS             complement(<1..135)
                     /gene="rpsF"
                     /locus_tag="VIR04_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY9459365.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYTAVITGNGGQVHRFEDWGR
                     R"
     gene            complement(317..1120)
                     /gene="folE2"
                     /locus_tag="VIR04_01055"
     CDS             complement(317..1120)
                     /gene="folE2"
                     /locus_tag="VIR04_01055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY9459366.1"
                     /translation="MNSLTDSTIALPDVQHTVDTRHMPIQRVGVRGVRHPMLIASVAG
                     PVLATVAQWELTVALPAREKGTHMSRFVALLEQYRATPMTPVVFCAMARAMLPLLNAE
                     QGDISASFPYFMKKTAPISEVESLMDYEVTWIARATAGAVEFELSVLVPVTSLCPCSK
                     AISRHGAHNQRSHVTVNAVFADPAAVDMDALIRIVEQQASCELWGLLKRVDEKFVTER
                     AYENPKFVEDLVRDVAAGLKGFTGLERLRIQAENFESIHNHSAYAAIGI"
     gene            complement(1249..3135)
                     /gene="dxs"
                     /locus_tag="VIR04_01060"
     CDS             complement(1249..3135)
                     /gene="dxs"
                     /locus_tag="VIR04_01060"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY9459367.1"
                     /translation="MPTVTLDQIHCPADLRALEPGELKEIADQLRAFVLQSVSKTGGH
                     LSSNLGTVELTIALHYVFNTPDDRIVWDVGHQSYPHKILTGRREQMAHLRQLGGISGF
                     PRRSESEYDAFGTAHSSTSISAILGMAVASRNLGLDKRQHIAVIGDGAMTAGMAFEAL
                     NNAGVTPGVNVLVVLNDNDMSISPPVGALNRYLARLMSGRFYANAKNVGRVVLQHVPP
                     VLELARRLEVHAKGMLSPATLFEELGFNYVGPIDGHDLSSLIPTLENLRALGGLQFLH
                     VVTKKGRGYKLAEADPVLYHGPGKFDLSVGIQKPKTPGRQTFSQVFGRWLCDMAQVDS
                     RLYGITPAMREGSGLIEFEQRFPERYFDVGIAEQHAITFAAGLACEGQKPVVAIYATF
                     MQRGYDQFIHDVALQNLDVTFALDRAGIVGADGASHAGNYDIAFLRCVPNMVVATPSD
                     ENELRLLLSTCYQHPGPASVRYPRGAGCGAQVRPGLDIVELGKGVVRRQGRRIAILGF
                     GALVPQGLEAAEALDATLVDMRFVKPLAGALLQTLAATHDAFVTLEEAAVMGGAGSAV
                     LEYLCANAIGIPVLQLGLPDRFIDHGEQAVLLEGLGLNAAGIEASIRARFPVPQVHAF
                     SRSR"
     gene            complement(3216..>3504)
                     /locus_tag="VIR04_01065"
     CDS             complement(3216..>3504)
                     /locus_tag="VIR04_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY9459368.1"
                     /translation="STLRQGLETYAQAVGLAFQVADDILDVTSDTATLGKTAGKDAAG
                     NKPTYVSIMGLIESRKMLESLRMQAHAALLPFGAPGTRLAEIADYIVGRDH"
BASE COUNT          857 a         1071 c          898 g          678 t
ORIGIN      
        1 acgacggccc cagtcttcaa aacggtgcac ttgaccgcca ttgccggtaa tcacggccgt
       61 atagcgctcg accatggcgg gcacttgctc gctttgatcg ggatgcacaa taaacactat
      121 ttcatagtga cgcataaaac cacactcctt aaggatgtct tttaacgcgg acataacggt
      181 tttgcgttaa aagggacagc ccgcccttgt tggcagacat taaaacaacc gaaagacaca
      241 aacggctgtt taaaagatgc aaaaagcaag ggtcgagcaa gaaaccgcta ataataccac
      301 gttggaaacg gctttactaa atcccgatgg cggcgtacgc tgaatgatta tgaattgatt
      361 caaaattttc agcctgaatc cgcagacgct ccagtcccgt gaaaccttta agcccggccg
      421 ccacgtcccg gacgaggtcc tcgacgaatt tcggattctc gtatgcgcgc tcggtgacga
      481 atttttcatc gacccgtttc aacagtcccc acaactcgca tgaagcctgc tgttccacta
      541 tacggataag cgcatccata tcgactgcgg ccgggtcggc aaatacggca ttaacggtta
      601 cgtgcgagcg ctgattgtgg gccccgtgcc gcgaaatagc cttggaacat gggcacaggc
      661 tcgtcactgg aactaaaacc gagagttcga attcaaccgc gccagccgtt gcgcgagcga
      721 tccacgtgac ttcataatcc atcaagcttt caacctcgga aatcggagct gtctttttca
      781 tgaaataagg gaaggaagcc gagatatccc cctgttccgc attcagcaac ggcagcatcg
      841 cgcgcgccat ggcgcaaaac acaacgggtg tcatgggcgt cgctcgatat tgttcgagca
      901 aagcgacgaa acgcgacata tgcgtgccct tttcccgcgc cggcaacgcc acagtaagtt
      961 cccactgggc caccgtcgcc agcacgggtc cggcaaccga agcaataagc atgggatggc
     1021 gcacaccgcg aacacccacg cgctggattg gcatatggcg cgtatcgacc gtgtgctgca
     1081 cgtcaggcag ggcaatcgta ctatcagtca atgaattcat aacaaaaaca cccaaagcgc
     1141 caggcaagcg gcaaggcgcc aataaagaga agaatcattt attgcgtggt cacacatagt
     1201 gggcaaaacc ctaagaccac aacggaatca cacccattag tgatcagttt agcgcgaacg
     1261 cgagaaagcg tgcacctgcg gcacggggaa acgggcccgg atcgacgctt cgatgcccgc
     1321 cgcattaaga cccaggcctt ccagcaagac cgcctgctcg ccatggtcga tgaaacgatc
     1381 gggcaggcca agctgcaaca cagggatacc aatcgcgttt gcgcaaaggt attccagcac
     1441 ggcgcttccc gcaccgccca tgacggcggc ttcttcaagc gttacgaacg cgtcgtgtgt
     1501 cgcggccaac gtttgcagca gcgcgccggc cagcggtttg acaaaacgca tgtcaaccag
     1561 cgtggcatcc agggcttcag ccgcttcgag gccctgtgga accagggccc caaaccccaa
     1621 gatggcaatt cgccgcccct gtcgcctgac cacccccttg cccagttcaa caatgtccag
     1681 tcccggccgc acttgcgctc cacagccggc accgcgcgga taccgcacgg aagcaggccc
     1741 cgggtgctga taacatgtgc ttaacaataa acgcagctcg ttttcatccg acggtgtggc
     1801 cacaaccata ttcggcacgc aacgcaaaaa cgcgatatcg taattacccg catgcgaggc
     1861 cccgtcggcg ccgacaatgc ccgcgcggtc cagtgcaaaa gtcacatcca gattttgcag
     1921 cgccacatca tgtatgaact gatcatagcc acgctgcata aaggtggcgt aaatggccac
     1981 aaccggcttc tgcccttcgc aagcaagacc cgccgcaaac gttattgcat gctgctcggc
     2041 gattcccaca tcgaagtaac gctcgggaaa acgctgctca aattcgatca gtccgctgcc
     2101 ttcgcgcatt gccggggtaa tgccgtacag gcggctgtcc acctgcgcca tatcgcacaa
     2161 ccagcgcccg aatacctggc tgaacgtctg cctgccaggc gtctttggct tttggattcc
     2221 cacgctcaga tcaaatttgc ccgggccgtg gtacaaaacc gggtccgctt cagccagctt
     2281 gtagccgcgg ccctttttcg tgacaacatg caaaaactgc aatccgccca gagcgcgcaa
     2341 attttccagt gtgggtatca gggaactcag gtcatgcccg tcgataggcc ccacgtaatt
     2401 aaagcccaat tcctcgaaca aggtcgccgg cgacagcatt cctttggcgt ggacttcaag
     2461 gcgccgagcc aattccagta ccggtggcac gtgttgcaac acgacccggc ccacattctt
     2521 ggcattcgca taaaaccgcc ccgacattaa acgggccaga taacggttaa gcgctcccac
     2581 cggcggggaa atcgacatgt cgttgtcgtt gagcaccacc agcacgttga cgccaggcgt
     2641 aacacccgca ttattcaagg cttcgaatgc catgcccgcc gtcatggccc catcgccaat
     2701 aacagcaatg tgctggcgct tgtcgagtcc cagatttcgc gatgccaccg ccatgcccag
     2761 aatcgctgat atcgacgttg acgaatgcgc tgttccaaac gcgtcgtact ctgactcgct
     2821 gcgcctcgga aagccggaaa tcccgcccaa ctgacgcaag tgagccatct gctcgcggcg
     2881 gccagtcagg attttatgcg gatacgattg atggccaacg tcccacacga ttcgatcgtc
     2941 gggcgtattg aatacataat gcagcgcaat cgttaattcc accgtgccca ggttggacga
     3001 caagtgcccc ccggtcttcg acaccgattg caacacgaat gcgcgcaact gatctgcgat
     3061 ctctttaagc tcgcctggct ccagcgcgcg caagtcggct gggcaatgaa tttgatcgag
     3121 agtaacggtt ggcatactgg taaaaaataa cggcctgagg tctattctaa aacttatggg
     3181 gaatgtatac cgcaacgacg ttcaaaatcg aagattcaat ggtcgcggcc cacaatgtaa
     3241 tcggcaatct cggccaggcg cgtgccaggc gccccgaacg gcaacaaagc cgcatgagcc
     3301 tgcatgcgca atgattcaag catcttgcgc gattcgatca gccccatgat cgacacgtag
     3361 gtcggcttat ttcccgccgc gtccttgccg gccgtcttgc ccagtgtcgc ggtatcggac
     3421 gtgacatcca gaatatcgtc ggccacctga aacgcaagac cgacagcctg tgcatacgtt
     3481 tcaagcccct ggcgaagagt ggag
//
LOCUS       DATCXU010000050         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_616745,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000050 DATCXU010000000
VERSION     DATCXU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616745"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="rplA"
                     /locus_tag="VIR04_01070"
     CDS             complement(<1..694)
                     /gene="rplA"
                     /locus_tag="VIR04_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY9459369.1"
                     /translation="MSKPSKRTAAIQAKIDPKKLYSVKDAIALIKETATAKFDESIDI
                     AVQLGIDPKKSDQLVRGSVVLPAGTGKVVRVAVFAHGEKAEAAKAAGADIVGLEDLAE
                     SIKAGKLDFDVVIASPDTMRVVGALGQILGPRGLMPNPKVGTVTPDVVTAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFGAEQLQSNLNALVDALNKARPVAMKGIYLRKLAVSSTM
                     GGGARVEIASLTA"
     gene            complement(691..1128)
                     /gene="rplK"
                     /locus_tag="VIR04_01075"
     CDS             complement(691..1128)
                     /gene="rplK"
                     /locus_tag="VIR04_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY9459370.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQGLEPGLPIPVIITAYTDKSFTFIMKTPPASILIKKASGVQKGSANPSLNKVGTLT
                     RAQAEEIAKAKAPDLTAADLDAAVRTIAGSARSMGITVEGVEA"
     gene            complement(1194..1727)
                     /gene="nusG"
                     /locus_tag="VIR04_01080"
     CDS             complement(1194..1727)
                     /gene="nusG"
                     /locus_tag="VIR04_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY9459371.1"
                     /translation="MSKRWYVVHVYSGMEKSVHKALLERIERAALQTSFGRILVPSEE
                     VVEVKGGKKSISERRIFPGYVLVEMDLTDETWHLVKNTNRVTGFLGGAGNRPAPISDL
                     EVGKILSQMEEGVEKPKPKVLFEVGELVRVKDGPFADFNGNVEEVNYEKSKVRVSVTI
                     FGRATPVELDFGQVEKS"
     gene            complement(1737..2117)
                     /gene="secE"
                     /locus_tag="VIR04_01085"
     CDS             complement(1737..2117)
                     /gene="secE"
                     /locus_tag="VIR04_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY9459372.1"
                     /translation="MSKTNVETITSTADRVKVGLAVLVIIAGVVGYEMLTDQSGPARV
                     GLFLASVVVATIILWFSELGRRTVSFARDSYYEVKRVVWPDRKESTRMTIIVFLFVVA
                     MAFLLWVVDKLLEWIIYGVLLGWK"
BASE COUNT          482 a          652 c          580 g          508 t
ORIGIN      
        1 aagccgtgag cgaagcaatt tctacacgag cgccgccacc cattgtggag gacacggcaa
       61 gcttgcgcag ataaatacct ttcattgcaa ccggacgcgc cttgttcagg gcgtcgacca
      121 gcgcgtttaa attcgactgc aactgctcgg cgccaaatga agcgcggcca atcgtcgcat
      181 gaacaatgcc tgccttgtca gtacggtatt gcacctgccc cgctttcgcg tttttgacgg
      241 cagtcacaac gtcgggggtc accgtgccca ctttcgggtt cggcatcaag ccgcgagggc
      301 ccaatatttg acccagagcg ccaaccacgc gcatggtgtc gggcgatgca atgaccacgt
      361 cgaaatccag cttgcccgcc ttgatgcttt cggccagatc ttccaggcca acgatgtcgg
      421 caccggcggc cttggccgct tctgcctttt caccatgcgc gaacacggcg acacgcacca
      481 ctttgccggt ccccgcgggc agcacaaccg agccgcgtac aagctggtcg gatttcttgg
      541 gatcaatacc caactgcacg gcgatatcga tggattcgtc gaatttggca gtcgccgttt
      601 ccttgatcag ggcgatggca tctttaacgg aatagagctt cttgggatca atttttgcct
      661 gtatcgcagc ggtgcgtttg cttggcttgc tcatgcttcg actccttcga cagtaattcc
      721 catactgcga gcgctacccg caatcgtacg cacggcggcg tcgaggtcgg cggcggtcaa
      781 gtcgggcgct ttggcttttg caatttcttc ggcttgcgcg cgagtcaggg tcccgacctt
      841 gttaaggctg ggattcgccg accctttttg cacacccgac gccttcttga tcaggatgga
      901 cgcgggcggc gtcttcatga tgaacgtgaa gctcttgtcg gtgtaggccg tgataattac
      961 gggaatgggc aagcccggct ccagaccctg agtcttggcg ttgaatgcct tgcaaaattc
     1021 catgatgttc aggccgcgct gaccgagcgc cggcccaatg ggtggcgatg gattcgcctt
     1081 gcctgcaggc acctgcagct tgataaaacc gacgattttt ttcgccatag tttgctcctt
     1141 gcgggttctt gcgccaaccg ccgcttcgcg gcaaccggct ccccggggtt aaatcaggac
     1201 ttttcgacct gaccaaaatc gagttcaaca ggggtggcgc ggccaaatat cgtgaccgaa
     1261 acccgcacct tgcttttctc gtagttaact tcttcaacgt tgccattaaa gtcggcaaag
     1321 ggaccgtctt taacgcgaac cagctcacct acctcgaaca gaaccttggg cttcggtttt
     1381 tcgacgcctt cttccatctg agacagtatc ttgccgactt caagatcgga tatgggcgcc
     1441 ggtcgattcc ccgccccgcc caaaaacccg gtgacccgat tggtgttctt caccagatgc
     1501 caggtctcgt cggtaagatc catttcaacc agtacatagc cgggaaaaat acgccgttct
     1561 gaaattgatt tcttgccgcc cttgacctcg accacttctt cggacgggac cagaatacgg
     1621 ccaaaagagg tttgtaatgc agcacgctcg atgcgctcca gcagcgcttt atggacactt
     1681 ttttccattc ctgaatagac atggactaca taccaacgtt tgctcatgtg tgccctttat
     1741 ttccagccga gtaacacgcc gtatatgatc cactcaagga gcttatccac gacccataac
     1801 agaaaagcca tcgcgacgac gaaaagaaaa acgataatcg tcatgcgggt tgattcttta
     1861 cggtcgggcc aaacaacgcg cttgacttcg taatacgagt cgcgggcgaa actgaccgta
     1921 cggcgaccga gttcgctgaa ccaaagtatg atcgtcgcaa cgaccacgct ggcaaggaac
     1981 aaacccaccc gagccggacc ggactggtct gtgagcatct cgtacccgac gaccccggca
     2041 ataatgacaa gcacggccaa acccacttta acgcgatcgg cggtgctggt gatggtttct
     2101 acattagtct tcgacatatt ccgacgagtg aatccgctat atacagtcta cgatgcagta
     2161 ttggcggcct ggcaggggca gaaggaatcg aaccttcaac cttcggtttt ggagaccgac
     2221 gc
//
LOCUS       DATCXU010000051         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_702484,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000051 DATCXU010000000
VERSION     DATCXU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702484"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VIR04_01090"
     CDS             <1..293
                     /locus_tag="VIR04_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEY9459373.1"
                     /translation="GLQVAEEVFADRSYQSDGTLTSRLQPNAMIVDINVSIRQVLRMI
                     REGKIRTVQGSDIDVRPDTLCIHGDQPGAPAFAKAIRQALEAEGIAVRAVGA"
     gene            complement(295..1806)
                     /locus_tag="VIR04_01095"
     CDS             complement(295..1806)
                     /locus_tag="VIR04_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY9459374.1"
                     /translation="MTQTSIHSALSHTEVRSVIIGLMLAILLGALDQTIVSVSLPMMS
                     ADLQGADLLAWVVSGYLIAVAVATPIYGKLGDLYGRRVMLSSAIIIFLLGSIGSALAE
                     SMTTLVAARIGQGLGGGGLISVAQAIIADVVAPRERGRYQGYISAAFAVASVSGPLAG
                     GLLTQYLSWRWVFWINVPLGLLALFISRRALALLIVPHIKRPIDYTGAVLLSAGLSAL
                     LIGVTRVGQGTSWVDSDNLLLFAAALACLAAFVWQEQRSAEPIIPLSLFRNPTVAISC
                     AILFIAFFQIIALSVLIPLRLQMLTDLGADGAALRLVPLSLAIPTGAFFGGRMTTRTG
                     HVKKIQLVGAVIVPLAVLALAFIDVHATLTGLVFMALAGFALGLQLPTSTVAVQNAVP
                     HHFVGIATAVSVFCRSLGAALGIAILMAALLATMQASAPAIEASLSGAEIIKTMVGDA
                     ATQATAIAMQLKLPVQDAFQRIFIISALISLTSCVLGLLMSDETLGDQLPEIE"
     gene            2068..3462
                     /locus_tag="VIR04_01100"
     CDS             2068..3462
                     /locus_tag="VIR04_01100"
                     /inference="COORDINATES: protein motif:HMM:NF037270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459375.1"
                     /translation="MTDLTIRVAVIGPAHLNTQDPSVQWHVSVASLLASEQCDVVLLD
                     LSGSQAGQALRELRREPNYRYLLIYCRRDQDAFCEALGDGEPPPDRDGLVTAWTLWQA
                     RYGLLDKWRFDEYFETRVLAWLWLRLNRQVSAVCNPALAQHYEYPLLDVLAGEDRINS
                     FVWLQLMNDQGWLEERTLIDRMRLCTQCGSGRLNYIDVCPECQSIAIARQPALHCFMC
                     GHVSAQENFLKDGVLLCPNCLSRLRHIGTDYDRPIENYRCRGCQAFFVDAMVQARCLD
                     CGAMHGTDALRVHEVHTYGLTAAGRLRCREGFQSSSASEERFNRLGLLGVRSFHHSLD
                     WMLEIQRRYRQPVFSLLGLRFVHLSTAIARLGEQHGRALVDTLIERLFESMLETDRYT
                     RINEECLCLLLPQVDAVDLARVKRRLEKLSDLFVAPEAHEIELRMVDFTAPQDLLENE
                     NGELLMARLMDGLS"
     gene            3472..4797
                     /locus_tag="VIR04_01105"
     CDS             3472..4797
                     /locus_tag="VIR04_01105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9459376.1"
                     /translation="METFNIWQGFFQELSRADGVSRLFLMLFPYFLLFELPVNMMVLL
                     GVLHWFVRKRSTPPRKSFYHPNVSCIITCYSEGLDVQKTLISMAEQTYPGSIEIIPVI
                     DGASANKETLAAVRGFRLDPALYPRRHMRPIAKWQRGGRVSSLNAGMAHATGEIIMVL
                     DGDTSFDNTMVSAIVPHFMDPDVPAVAGSLRVRNVWASLTTMIQALEYLLSIHMFKIG
                     LAEWNIVNNVSGAFGAFRRDFLLRVGGWDTHTAEDLDMTLRLKSYSRRRTIHIPFEPE
                     AVGHTDVPVTFRQFLMQRLRWDGDLFFMYIRKHKHNLTPRLLGWPNFLMILFTGLFFQ
                     LVVPLIIVGYSILSFFLLPPATLFMVFTLMYLLYLLITGVMFTGMLLMVSERPAQDLR
                     LALVVPLFPLFMIVLRCWSAVALLNELLRRGHEESSMAPWWVLKRGTKF"
BASE COUNT         1018 a         1320 c         1409 g         1135 t
ORIGIN      
        1 caggtctgca agtggctgaa gaagtgttcg ccgatcggtc ttaccaaagt gatggaactc
       61 tgacgtcgcg ccttcagccc aacgccatga ttgtcgatat caatgtgtcg attcggcagg
      121 ttctgcgcat gatccgggaa ggaaagatcc gaacggtgca aggcagcgat atcgatgtcc
      181 gtcccgatac gctgtgcata cacggcgatc aaccgggagc gccggccttc gcgaaagcaa
      241 tccgtcaggc gctcgaggcc gaaggcattg ccgtgcgcgc ggtcggcgca tagcttattc
      301 tatttcgggc aattgatcgc caagcgtttc gtctgacata agcagcccca gcacgcacga
      361 tgtcagcgag ataagtgcac tgattataaa tatccgctga aacgcatctt gtacgggcaa
      421 ttttaattgc atggcgatcg cagtagcctg cgtggcggca tcgccaacca tggtttttat
      481 aatttcggcg cccgatagcg atgcctcgat tgcgggggcg ctggcttgca tggtagccag
      541 aagcgccgcc atcaatatgg caatgcccag cgcggcgccc agcgagcggc aaaaaacgct
      601 gacggccgtg gcgatgccga cgaaatgatg cggcaccgcg ttttggaccg caactgtcga
      661 agtgggcaat tgcagtccca gcgcgaaccc cgccaaggcc atgaatacca ggcccgtcag
      721 tgtcgcgtga acgtcaatga aggccagcgc cagaaccgct agtggcacga tcaccgcgcc
      781 aaccagttgg atttttttga catgcccggt tcgcgtggtc attcgtccac cgaagaatgc
      841 accagtcgga atggccagcg atagcggcac caaccgcagg gcagcgccgt cggcgccgag
      901 atccgtgagc atttgaagcc gtaacgggat gagcaccgac agcgcaataa tctgaaagaa
      961 ggcaataaat aatatcgcgc aggaaatggc cacagtggga tttcgaaata gcgaaagcgg
     1021 gatgatcggt tcggcgctgc gttgttcttg ccagacgaaa gccgccagac aggcaagggc
     1081 cgcggcaaac agcagaagat tatcgctgtc cacccacgag gtgccctgac cgacgcgcgt
     1141 taccccgatc agcagcgccg acagacctgc cgataaaagc accgcgccgg tgtaatcgat
     1201 gggtcgcttt atgtgcggca caattaaaag cgccagcgcc cggcgcgaaa tgaaaagcgc
     1261 cagtagtccc agcgggacat tgatccagaa tacccagcgc catgaaagat actgggtcag
     1321 cagcccaccc gccaacgggc ccgacacgct ggcgacggca aatgcggcgc tgatatagcc
     1381 ttgataacgg ccgcgctcgc ggggcgcgac gacgtctgca ataatggctt gggcgactga
     1441 gatcaggcct cctcctccca acccttgacc aatgcgcgcg gcgaccagcg tagtcattga
     1501 ctcagccagc gcgcttccaa tcgaccccag caggaaaatg ataatggccg atgacagcat
     1561 gacccgccgc ccgtacagat ctcccagctt gccgtatatg ggcgtggcaa cggccaccgc
     1621 aatcaaatag ccggacacca cccacgcaag caggtcggcg ccttgaaggt cggccgacat
     1681 catgggcaac gataccgaga ctatggtctg gtccagggcg cccagcaaaa tcgctagcat
     1741 caggccaatg atgacggaac ggacttccgt gtgcgataac gccgaatgga tgctggtttg
     1801 agtcatggcg ccatattcta gttcatcccc agcgcctgcg gtgcgacaag caatttttca
     1861 actgaatcat atggttgcag ctctgctacg catcgtctac aacccgtcac agtttttagt
     1921 gggtcgaatc gttatttaac acatttaccc tatgccggga ttaacgtgtc tctataaaat
     1981 taattcgtcc ttcgcagcac agcgtgggct tgatcataag cgccgcgacg atggcgcagt
     2041 ggcgttaatc tcctcggcaa gaaggttatg actgatttga ccatccgcgt cgctgtcata
     2101 ggacccgcgc atttaaacac gcaggacccc agcgtccaat ggcatgtctc ggttgcaagc
     2161 ctgctcgcca gcgaacagtg cgatgtggtt ttgcttgact tgtcaggatc tcaggccggc
     2221 caggccttgc gtgagctgcg gcgcgaaccc aattaccgct atttattgat ttattgccgc
     2281 cgggatcagg acgccttttg cgaagcattg ggcgatggcg agcccccacc cgatcgtgat
     2341 ggtttggtga ccgcttggac gctgtggcag gcgcgttatg gtctgctcga taaatggcgc
     2401 ttcgacgagt atttcgaaac gcgtgtgctg gcctggctat ggttgcgctt gaacagacag
     2461 gtgagcgcgg tatgcaaccc ggcgttggct cagcattatg aatacccatt gcttgatgtc
     2521 ctggccggcg aggaccggat taattctttc gtttggcttc agttgatgaa cgatcagggc
     2581 tggcttgaag agaggactct gattgatcgc atgcgcctgt gtacgcagtg cggttccggg
     2641 cgcctcaact acattgatgt ctgcccggaa tgtcaatcta tcgccatcgc acgccaaccg
     2701 gcgctgcact gcttcatgtg cggccatgtg agcgcccagg agaattttct taaagacggc
     2761 gtactgctgt gtccgaattg tttaagccgg ctgcgccaca ttggcacgga ttacgaccgg
     2821 cctatcgaaa attatcgctg tcgcggttgc caggcatttt tcgtcgatgc gatggtgcag
     2881 gcccgttgtc tggactgcgg agcgatgcat gggaccgacg cgctacgcgt tcacgaggtg
     2941 cacacctatg gtctgaccgc ggcgggccgc ttgcgttgtc gtgaaggttt tcaaagcagt
     3001 tcagcctcgg aagagcggtt taatcgtttg ggtttgctgg gcgttcgatc ttttcatcat
     3061 tcgctggact ggatgctgga aatccagcgg cgctaccgcc agccagtgtt ctcgttattg
     3121 gggctgcgat ttgttcatct gtccacagcc atagcgcgcc tgggcgaaca gcatgggcgc
     3181 gcgttggtcg acactttgat tgaacgcttg tttgagtcga tgcttgagac ggatcgctat
     3241 acccggatca acgaagaatg cctttgcctg ttgttgccac aggtcgatgc tgtggatctg
     3301 gcccgggtga aacggcgcct ggagaagtta tcggatctgt ttgttgcgcc cgaggcgcac
     3361 gaaattgaat tgcgcatggt ggatttcacc gctccgcagg atttgctgga gaacgagaac
     3421 ggcgaactgc ttatggcccg tttaatggat ggattgagtt aaggggatgt gatggaaact
     3481 ttcaatatct ggcaagggtt tttccaggaa ttgtcccgcg ccgatggggt gagccgtctg
     3541 tttctcatgc tgtttccgta ttttttgtta tttgaattgc ctgtgaatat gatggtgctc
     3601 cttggcgtgc tgcactggtt cgtgcgcaag cgcagcaccc ccccgagaaa gtccttttat
     3661 caccctaatg tttcgtgcat tatcacgtgc tacagcgaag gtctggacgt ccagaaaacc
     3721 ttgataagca tggcggagca gacgtatccc ggatcgatcg aaattattcc ggttattgac
     3781 ggggccagtg ccaacaagga aacactggcc gccgtgcgcg gctttcgtct tgatcccgct
     3841 ttatacccgc gtcgtcatat gcgccccatt gccaagtggc agcggggcgg gcgcgtgtcg
     3901 tcgctgaatg ccggcatggc gcacgccacc ggagagatca tcatggtgct cgatggcgat
     3961 acttcgtttg acaacactat ggtaagcgcc atcgtgcccc acttcatgga tccggatgtg
     4021 cccgctgtgg cgggcagttt gcgcgttcga aacgtatggg cgtccctgac caccatgatc
     4081 caggcgttgg agtacttgct atctatacac atgttcaaga ttggactggc cgaatggaac
     4141 atcgtgaaca acgtctcggg cgcctttggc gcgtttcgtc gcgattttct tttgcgtgtc
     4201 ggggggtggg acacgcatac ggccgaggat ctggacatga ccttgcgcct gaaaagctat
     4261 tcgcggcgcc gcacgattca tattccgttc gagcccgaag ctgtcggcca taccgatgtg
     4321 ccggtcactt tcagacaatt tctgatgcag cgcctgcgtt gggatggcga cctgtttttc
     4381 atgtatatcc gcaagcacaa gcacaatctg accccccgtc tgctgggctg gcccaacttc
     4441 ctgatgatct tgttcacggg ccttttcttt cagttagtgg tgccactcat tattgtgggt
     4501 tacagcattt tgtccttttt cctattgccg ccggcgaccc tgttcatggt gttcacgctg
     4561 atgtacctcc tttatttatt gattaccggc gtgatgttta ccggcatgct gctgatggtt
     4621 tccgaacgac ccgcgcagga tctgagactt gcattggtcg tcccgctgtt tccactgttc
     4681 atgattgtgt tgcgttgctg gagtgcggtc gcgttgctta acgagctgct gcgccgtggc
     4741 cacgaagaaa gcagtatggc gccatggtgg gtgctgaaaa gagggacgaa attctaatga
     4801 aaaacacttt ctttcttatg gcgatacttt ttttttcggt attcgcgaac gctgatcccc
     4861 tgccgctggc ggcattgatg aa
//
LOCUS       DATCXU010000052         5191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_852964,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000052 DATCXU010000000
VERSION     DATCXU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852964"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="rfaD"
                     /locus_tag="VIR04_01110"
     CDS             <1..923
                     /gene="rfaD"
                     /locus_tag="VIR04_01110"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY9459377.1"
                     /translation="DIMVVDDLTDGDKFLNLTDLQIADYLHKDDFRQRVNRGQFAGVE
                     AVLHQGACSDTTERDGRYMMDNNYRVTLELFQFCQQQKIPLLYASSAAVYGAGPVYTE
                     QLLYESPLNVYGYSKFLFDQVLRRHLHSLSSPVIGLRYFNVYGPNEQHKGRMASVALH
                     NMNQFRTEGHVRLFGGWDGYADGAQRRDFVFVDDVVSVNLHFLDHPGKSGIFNCGTGR
                     AQPFNDVASTVVNTLRAQAGQPELALSELVERKLIRYISFPDDLKGRYQNHTQADLTQ
                     LRLAGYTQPFCDVRTGVSAYVKGLLEQGRL"
     gene            1097..1636
                     /locus_tag="VIR04_01115"
     CDS             1097..1636
                     /locus_tag="VIR04_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY9459378.1"
                     /translation="MNPFTEPTVARPWTDDQRAKFGAASFPRKEPEVKAARSRPASPK
                     KRQRPFSGSAFGIIALAASLSLFLPAPAHALDVNTATQVELRALRGIGSKTAQIIIEE
                     RSRGGRFESFEDLSDRVKGIGPKKAASLQAAGLTLGGQGAAEPAGRRESGTPQAVKSA
                     SGVTKGQKPAQSRTFKLRP"
     gene            1708..2613
                     /gene="cysM"
                     /locus_tag="VIR04_01120"
     CDS             1708..2613
                     /gene="cysM"
                     /locus_tag="VIR04_01120"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY9459379.1"
                     /translation="MKKYTTIEDTIGSTPLVRLQRIPGPSQEQRGNVILAKLEGNNPA
                     GSVKDRAAASMIRHAEQRGEIKPGDTLIEATSGNTGIALAMMAAVRGYKMVLIMPDDL
                     SVERRAAMTAYGARLILTKADQGGMEYARDLAGKMQSEGKGKVLDQFANADNTQAHID
                     TTGPELWQQTDGQITHFVSAMGTTGTIMGVSTYLRSRNPRIQIVGAQPAEGSKIPGIR
                     KWSEEYQPKIYHPELVDSFELIKQSEAEEMARRLAAEEGIFGGISSAGALVAALRVAA
                     RVENATIAFIVCDRGDRYLSTGVFN"
     gene            complement(2606..3799)
                     /gene="mltB"
                     /locus_tag="VIR04_01125"
     CDS             complement(2606..3799)
                     /gene="mltB"
                     /locus_tag="VIR04_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY9459380.1"
                     /translation="MSKPVRVLQAAVAFALLSGCATPQSTALSSTSSAPQATPNSERA
                     TPQQTPGAPLAAPRAPIKASVGSSDNRNSFLDANGELEPDVQAFALEVAQTREVPLNY
                     VQDILRRAQYNATVARLMTPAKTRIRRSWITYRDRVVEPIRIKAGVEFWTQYRSTLDQ
                     TASTYGVPPSIIVAIIGVETIYGRQTGNFLVLDALATLGFRNPDTARPERSRLFRDQL
                     ADLILLDYQKKIDGTQVKGSFAGAIGLPQFMPGSLMRYAIDGDNDGQINLLDSAPDAI
                     ASVARFLKLHGWQPGLPVFAPVVLPANASSLVAGGLKPSYDWAQLKTEGAALKPGTGP
                     GAWQSSRLGVIDLPDEPRNLDEYRTGTPNFFAITQYNRSYFYAASVADLAEALAGRMG
                     YGGPN"
     gene            3810..4733
                     /locus_tag="VIR04_01130"
     CDS             3810..4733
                     /locus_tag="VIR04_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY9459381.1"
                     /translation="MQTLYITHPACRLHEMGQWHPESPDRLAAINDQLMASGLLNFLA
                     GKQAVMASDTDILRVHTADYLAYLLSHAPSRGYFAIDPDTLMNAHTLDAARAAAGSGI
                     TAVDMIMSEQAKTAFCAVRPPGHHACPDRAMGFCFFNNLAVAAAYALEKYALNRVAIV
                     DFDVHHGNGTEEIFSGDPRVLMCSFYQRGLFPDTGVESHAGNMLNIPVDAYTGGDALR
                     QLVHTCWMPRLKAFEPEFIFISAGFDAHREDTIGQLGMVEADYAWITRQIVASADASS
                     LGRVLSFLEGGYNLSALGRSAVAHVRALAAL"
     gene            4773..>5191
                     /locus_tag="VIR04_01135"
     CDS             4773..>5191
                     /locus_tag="VIR04_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY9459382.1"
                     /translation="MKVLVPVKRVVDFNVKVRVKSDRSGVDIANVKMSMNPFDEIAIE
                     EAMRLKEKGAASEVVAVSCGVAQCQETLRTAMAIGANRGILVQTDVELQPLAVAKLLK
                     ALVDKEQPRLVILGKQAIDDDANQTGQMLASFLNWPQA"
BASE COUNT         1123 a         1374 c         1550 g         1144 t
ORIGIN      
        1 ccgacattat ggttgttgat gatttgaccg acggcgacaa gtttcttaat ttgaccgatc
       61 tgcaaattgc cgattatttg cataaagatg attttcgcca acgggtgaac cgcggccaat
      121 ttgcgggcgt ggaagctgtc ttgcatcagg gggcctgctc ggatacgacc gagcgtgatg
      181 gccgctacat gatggacaat aattatcgcg tgacccttga gctgtttcag ttttgccagc
      241 aacagaaaat accgttgctg tatgcctcgt cggcggcagt ttatggcgcc ggcccagtgt
      301 ataccgagca attgctctat gagtctcccc tgaatgtgta tggctattcg aagttcctgt
      361 ttgatcaggt gctgcggcgc cacctgcatt cgctaagctc cccggtgatc ggactgcgct
      421 atttcaatgt atacggcccc aacgagcaac ataaaggccg catggcttcg gtggccttgc
      481 acaacatgaa tcaatttcgc accgagggtc acgtgcgctt gtttggcggg tgggacggtt
      541 acgcggatgg cgcccagcgt cgcgattttg tgtttgtgga cgatgtggtc agcgtcaatt
      601 tgcatttttt ggaccacccc ggaaagtcgg ggattttcaa ttgcggcacg gggcgcgctc
      661 aaccgttcaa tgatgttgcg agtactgtgg tgaacaccct gcgcgcgcag gccggccagc
      721 cggagctcgc cttgtcggaa ttggtagaac ggaaattgat ccgttatatt tcttttccgg
      781 atgacctgaa gggacgctat caaaaccata cgcaggcaga tctgacgcag cttcgcctgg
      841 cgggctatac ccagccattt tgcgatgtac gcacgggcgt atcggcttat gttaaaggcc
      901 ttctggagca aggccgcctg tagtgcgatg gattccttcg gcacgaattg cctgcgcggc
      961 ccgcgtatgt ttacgcatag ataattgagg gtgtttggcc tgtttcgttt aaggcattgc
     1021 tccgatcata gataagttga attgccgttc gagcgcggtt cggctcgaac tttatcttat
     1081 ctttcggaga ctccttatga acccattcac cgagcccact gttgcccgtc catggaccga
     1141 tgaccagcgc gccaaattcg gcgccgcgtc ctttccgcgc aaagagccag aggtaaaagc
     1201 cgcccgtagc cggccggcct ctcccaagaa gcgacagcgg cctttttcag gttcggcgtt
     1261 tggaatcata gccctggcgg ccagcctgag cctgttttta ccagcgccgg ctcacgccct
     1321 ggatgtcaat acagccacgc aggttgagct gcgggcgctt cgcggaatag gctcaaaaac
     1381 ggcgcaaatc attatcgagg aacggtctcg cgggggtcgc tttgaatcat ttgaagattt
     1441 gtccgatcgg gttaaaggca ttggtccgaa aaaggccgcg tcgctacagg cggcgggctt
     1501 gaccttgggc ggtcaagggg cggccgaacc agcagggcgg cgggaatcgg gtacgccgca
     1561 agcggtaaag tccgcctccg gcgtgacgaa aggtcaaaag ccggctcaat cacgcacttt
     1621 caagctgcgc ccctagcgcc tggaaaaacc aagcgcggat cagggtcgtt caagcccaca
     1681 gtaagcggtt cggtgtatga tttccgcatg aaaaaataca ccactattga agacacgata
     1741 ggttcgacgc cgctcgtgcg tttgcagcgc attccgggac cgtcgcaaga acagcgcggc
     1801 aacgttatcc ttgcaaaact cgaaggcaac aacccggctg gctcggtaaa agaccgcgcc
     1861 gccgcttcca tgattcgtca cgccgaacaa cgaggcgaga tcaagccagg cgacacgctg
     1921 atcgaagcca caagcggcaa taccggcatc gcgctggcta tgatggcggc agtgcgcggt
     1981 tataaaatgg ttctcatcat gcccgacgat ttgtcggtcg aacggcgggc ggccatgacg
     2041 gcttatggcg ccaggcttat ccttaccaag gcggatcagg gcggcatgga atacgcccgc
     2101 gacctggctg gcaaaatgca atccgaaggc aagggaaagg tgctggatca attcgccaac
     2161 gcggataaca cccaggcgca tatcgacaca acaggcccgg agctatggca acaaaccgac
     2221 gggcaaatca cgcattttgt gagcgcaatg gggacgacgg gaaccatcat gggggtaagc
     2281 acctatttgc gcagtcgtaa tccgcgcatt caaatcgttg gcgcgcaacc tgccgaaggc
     2341 tcgaagattc caggtattcg gaaatggtcc gaagaatatc aacccaagat ttatcaccct
     2401 gagctggttg attcgttcga gctcatcaag caatccgagg cggaagagat ggcaaggcgt
     2461 ctggcggccg aagaggggat ctttggcggg atatcatccg ccggtgcgct ggtggcggca
     2521 ttgcgagttg ccgccagggt ggaaaatgcc acaatagcgt ttatcgtttg cgatcgcggc
     2581 gaccggtatt tgtctaccgg cgtgtttaat taggtccacc atatcccata cgcccggcca
     2641 gcgcttcggc aaggtccgcc acagatgcgg catagaagta gctgcggttg tactgggtga
     2701 tcgcaaaaaa gttaggtgtt cccgtgcgat attcgtcaag gttgcgcggt tcgtcgggca
     2761 ggtcgataac cccaaggcgc gatgactgcc acgcgccggg tcccgtgccg ggcttaagcg
     2821 ctgcgccctc tgttttcagt tgcgcccagt cgtagctggg tttcaagccg ccggccacta
     2881 aacttgaggc gttcgcaggc aaaacgaccg gtgcgaatac gggcaagccg ggttgccagc
     2941 catgcagttt taaaaaacgc gcgaccgacg caatggcgtc gggggcgcta tcaagcaagt
     3001 tgatctgccc gtcgttgtcg ccgtcgatgg cataacgcat caggctgcct ggcataaatt
     3061 gcggcaggcc aattgcgccg gcaaacgaac cttttacctg cgttccatcg atttttttct
     3121 ggtaatcaag caggatcaag tcagccagtt gatctcgaaa caaccgactg cgttccggtc
     3181 tggcggtatc ggggtttctg aaacccagcg tggccaaggc atcaagaaca agaaagttgc
     3241 cggtttgccg cccgtatatg gtttcaactc caatgatggc gacaataatt gacggcggca
     3301 caccgtaggt gcttgctgtt tggtcgagcg tggagcggta ttgggtccag aattcaacgc
     3361 ctgccttgat gcgaatgggt tcgacgaccc ggtcgcgata cgtaatccag ctacggcgaa
     3421 tccgcgtttt ggcgggcgtc atcagtctgg ctacggtcgc gttgtattga gcgcgtcgca
     3481 aaatatcctg cacatagttc agcggcacct ccctggtttg cgccacttcc agcgcgaacg
     3541 cttggacatc cggttcaagc tcgccgtttg catccaggaa gctgttgcgg ttgtctgaag
     3601 agccgacaga ggctttgatt ggagcgcgcg gcgcggccag tggagcacca ggcgtttgct
     3661 gtggggtggc gcgttcggaa tttggcgttg cttgcggcgc ggaagaggtt gacgacaatg
     3721 ccgtcgattg cggcgtcgcg cacccactta atagggcaaa agcgacagcg gcttgcaaaa
     3781 ctcgaacagg tttggacata atcaccttta tgcagaccct atatattacc caccccgcat
     3841 gccgcctgca tgaaatgggc caatggcatc cagaaagtcc tgaccgcctg gcggcgatca
     3901 atgatcaatt gatggccagc ggtctgctga actttctggc tggaaaacag gcggtgatgg
     3961 caagcgatac tgacattttg cgtgtgcaca cggcggatta tctggcatat ttgctttctc
     4021 atgcccccag tcggggctat ttcgccattg accccgatac gctgatgaac gcgcatacgc
     4081 ttgatgcggc gcgggcggcg gcggggtcgg gcatcactgc cgtcgacatg attatgagcg
     4141 aacaggcaaa gacggcattt tgcgcggttc gtccgccggg tcaccacgcg tgtcccgatc
     4201 gggcgatggg gttttgcttt tttaataacc tggcggttgc ggcagcgtat gccctggaga
     4261 agtatgcatt gaaccgcgtg gcgatcgtcg acttcgacgt acatcacggc aacggtaccg
     4321 aggaaatatt ttccggcgat ccaagagtgc tgatgtgcag cttttatcaa cgcggactgt
     4381 ttccagacac gggcgttgaa agtcatgccg gcaatatgtt aaatattccc gtcgatgcgt
     4441 acacaggcgg cgacgcgctg cgtcaactgg tgcatacgtg ctggatgcca aggcttaaag
     4501 cgtttgaacc ggaatttata tttatatcgg ccgggtttga tgcgcaccgc gaagacacga
     4561 tcgggcaatt aggcatggtt gaggcggatt acgcctggat aacacggcaa attgtcgcct
     4621 cggctgacgc aagctcgctc ggccgcgtgc ttagcttcct tgagggcggc tacaacctgt
     4681 cggcgcttgg gcgcagcgct gtcgcgcacg tccgggcgct tgcggcgttg tagaatgatg
     4741 aaatctctta aaagtcccct ggaggatgct caatgaaggt gctggtgcct gtcaagcgtg
     4801 tggttgactt caacgtcaag gtgcgcgtca agtctgaccg aagcggtgtt gacattgcca
     4861 acgtcaaaat gtcgatgaat cccttcgacg aaattgcaat tgaagaagcc atgcgcctga
     4921 aagaaaaggg cgcggcaagt gaagtcgttg ctgtgtcttg cggcgtagcg caatgccagg
     4981 agacgctgcg tacggccatg gcgatcggcg ccaatcgtgg catattggtg caaacggacg
     5041 ttgaattgca acctctggct gtcgcaaagc tgctcaaggc cctggtcgat aaagagcagc
     5101 cccgattggt tattttgggc aagcaggcca tagacgacga tgccaatcag acagggcaga
     5161 tgctggcttc attcctgaac tggccgcaag c
//
LOCUS       DATCXU010000053         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1005082,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000053 DATCXU010000000
VERSION     DATCXU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005082"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..834)
                     /gene="phoU"
                     /locus_tag="VIR04_01140"
     CDS             complement(109..834)
                     /gene="phoU"
                     /locus_tag="VIR04_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY9459383.1"
                     /translation="MSEHTNKQFHSDLETTRSLFLQMGGLVEAMVQGGMQVLATGDLS
                     LVDQVREREKEVNRLEVEIDELIGMLIARHQPTAIDLRLLLSVSKMLTDMERCGDEAE
                     KIAKVARRLYESETPYVPVIELSHMANCVTAMIRDALDAFARKDPLHAAKVVRNDKEV
                     DMEWKGSLRHIITYMIEDPRTISRSIDLIFIARSLERIGDHAKNMAERVIYMVRGDDV
                     RHTGVKNTERMARGELPDEENGI"
     gene            complement(928..1611)
                     /gene="rpiA"
                     /locus_tag="VIR04_01145"
     CDS             complement(928..1611)
                     /gene="rpiA"
                     /locus_tag="VIR04_01145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY9459384.1"
                     /translation="MLTQVELKRNVAQAALDYVLPRIKPDAILGVGTGSTVDLFIDVL
                     SAHKHRFLGATSSSERSSARLASLGIKVLDLNLVESMPMYVDGADEIDHALNMTKGGG
                     GALTREKIVASVAEKFICIADESKLVDCLGKFPLPVEVIPMARAVVARRLSELGAVVT
                     LREGFVTDNGCQILDAAGLRITAPAELEARISAIPGVVTCGLFAMAGADVALLATQNG
                     IKELCRAPS"
     gene            complement(1636..1998)
                     /locus_tag="VIR04_01150"
     CDS             complement(1636..1998)
                     /locus_tag="VIR04_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEY9459385.1"
                     /translation="MSLVKLYGLKKCGTCAKAMAWLEQHGIDYRFTDYRDHSVPANVL
                     TAWATDLGGWEKMVNRASMTWRNLPDERKSPVSPEQWLALIAEFPALIKRPVMVASNG
                     AVSVGFSEKKYGALAIQQ"
     gene            complement(1995..2798)
                     /locus_tag="VIR04_01155"
     CDS             complement(1995..2798)
                     /locus_tag="VIR04_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9459386.1"
                     /translation="MIQPNRILRVFVLTVAYSFLYVPIVCLMAFSFNDSAIMTSWSGF
                     SWKWYYELFKDQALLDAAGLSLQIAVLSATAATIIGTWIGYVLARMGHFRGFALFVGM
                     VSAPLVIPEVVLGISLLLMFVEMGDLFGWPDGNGMFTIWVGHVSLCTAYVAVVIQSRI
                     RDLDRSQEEAALDLGARPLKVFFAIIVPSIAPALLAAWLLAFTLSLDDVVVASFLSGP
                     GYTTLPLEVFSRVRLGLKPEINALATLFMLVVGICIIVANRIHWNRESK"
     gene            complement(2785..>3022)
                     /locus_tag="VIR04_01160"
     CDS             complement(2785..>3022)
                     /locus_tag="VIR04_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEY9459387.1"
                     /translation="LVFIPSVGEFVIPEMLGGANTLMMGRVMWTEFFNNADWPMASAI
                     TCVMVLLLLVPLVLFQRGQVKQLDADGRRRHDST"
BASE COUNT          784 a          883 c          732 g          623 t
ORIGIN      
        1 tggaggaggg catgctcttc gagcctgacg aatcccggaa gaagcctcac gcttcgacga
       61 tacaaacatc agattcccca agtgaccccg cacagaacga tctcttcact aaattccgtt
      121 ttcttcgtct ggcaactcgc cacgcgccat gcgttcggtg tttttaacgc cagtatggcg
      181 gacatcgtcg ccacgcacca tgtaaataac gcgttccgcc atgtttttgg cgtgatcacc
      241 aatgcgttca agcgagcgcg caataaagat cagatcaatc gagcgggaga tcgtccgggg
      301 atcctcgatc atgtacgtaa tgatatggcg caacgatccc ttccactcca tatcaacctc
      361 tttgtcgttg cgcaccactt tggccgcatg cagagggtct ttgcgcgcga acgcatccag
      421 tgcatcgcga atcatggccg ttacgcaatt cgccatgtgg ctaagttcga tcaccggcac
      481 ataaggtgtt tccgactcat agaggcggcg cgccactttg gcaatcttct cggcctcgtc
      541 gccgcaacgt tccatatcgg tcagcatctt tgaaaccgac agcaacagac gaagatcgat
      601 tgcggtgggc tgatgacgcg caatcagcat gccgataagc tcgtcaattt caacctcgag
      661 cctgttgact tccttttcgc gctcccgcac ctgatcgaca aggcttaagt cgcctgttgc
      721 cagaacttgc atgccgcctt ggaccatggc ctcgaccaat ccacccattt gcaagaaaag
      781 ggagcgggtc gtctcaagat cagaatgaaa ttgcttgtta gtgtgctcgg acatatcagc
      841 ctttcgtcat cactggagta aataaattca aacagcgcag actctgcgta ccaagattaa
      901 gaagtataga tattatttat gacgatatca tgacggcgcg cggcacagct cttttattcc
      961 attttgagtc gccagcaaag caacatcagc tccggccatg gcaaacagac cacaggtcac
     1021 aacgcccggt atggcgctga tccgggcctc taattcggcg ggggcggtaa tgcgcagccc
     1081 cgcggcatcc aggatctggc atccgttatc ggtgacaaaa ccctcacgta atgtaaccac
     1141 cgcgccaagt tcggacaacc ggcgcgcaac aaccgcacgc gccatcggaa tgacctcgac
     1201 aggcaaaggg aacttgccca gacagtcgac cagtttggat tcatcggcaa tgcaaatgaa
     1261 tttttccgcc accgaagcga cgattttttc gcgcgtcaga gcgccgccgc cgccttttgt
     1321 catattcaag gcatggtcaa tttcgtcggc tccgtccaca tacatgggca tgctttcgac
     1381 cagattcagg tcgagcacct tgatccccag tgacgccaaa cgtgcggaac tgcgttccga
     1441 actggatgtg gcgcctagaa accggtgctt gtgcgccgac agcacgtcaa tgaacaggtc
     1501 tactgtggag cccgtaccca ctccaagaat ggcatcaggc ttgatgcgcg gcaaaacata
     1561 atccagtgcg gcctgcgcga cgttccgctt gagttcaact tgggtaagca taaaaagacc
     1621 ttgatcggga aaaaattatt gctggatcgc cagtgcgccg tatttctttt cagaaaatcc
     1681 cacactgact gcgccgttcg aagcgaccat gaccgggcgc ttgatcagag cgggaaattc
     1741 ggcgattagc gccagccact gttcgggcga tacgggcgat ttgcgctcat cgggcaaatt
     1801 gcgccacgtc atggaggcgc gattgaccat cttttcccag ccgcccagat cagtcgccca
     1861 ggcagtcaat acattagccg gaaccgaatg atcgcgatag tcggtgaaac gataatcaat
     1921 gccgtgctgc tccagccagg ccattgcctt ggcgcacgtg ccgcactttt ttaaaccata
     1981 aagctttacc agactcattt cgactcccgg ttccaatgaa ttctatttgc cacaataatg
     2041 caaatcccaa cgaccagcat aaacaaagtc gccagcgcgt ttatctcggg ttttagccca
     2101 agccgcaccc gggaaaacac ttcaagcggc aacgtcgtgt agcctgggcc cgatagaaat
     2161 gaagccacca ccacgtcgtc aagcgacagt gtaaacgcga gcaaccacgc tgccagcaat
     2221 gcgggcgcga tagaaggaac gatgattgca aaaaacactt tcaagggacg cgcacccaaa
     2281 tcgagagcgg cttcttcctg cgatcggtcc aaatcacgaa tacgggactg aataacgacg
     2341 gctacgtagg ccgtgcacag actgacgtgc ccgacccaga ttgtgaacat tccattgccg
     2401 tcgggccagc cgaacaaatc gcccatttcc acaaacatca acagcaggga gatgcccagc
     2461 accacttcag gaatgaccaa tggtgcgctt accatgccga caaataaggc aaacccgcga
     2521 aagtgcccca tgcgcgccaa tacatagccg atccaagtcc cgatgattgt tgccgctgtg
     2581 gcgcttaaca cagcgatttg caacgacaat ccggcggcgt ccagcaaggc ctggtcctta
     2641 aaaagctcgt aataccattt ccaggaaaag ccggaccatg aggtcatgat ggccgaatca
     2701 ttgaacgaaa acgccatcag acagacgata ggcacatata agaacgaata ggccacggtc
     2761 aatacaaaaa cacgcagaat ccggttaggt tgaatcatgg cgcctgcgtc catccgcgtc
     2821 taactgcttc acttggccgc gttgaaataa cactaaagga accagcaata acaacaccat
     2881 cacgcacgtg atggccgatg ccatcggcca atcggcgtta ttgaaaaatt ccgtccacat
     2941 gacacgtccc atcattaacg tattggctcc gcccagcatc tccggaataa caaactcacc
     3001 caccgacggt atgaacacca gc
//
LOCUS       DATCXU010000054         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_219913,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000054 DATCXU010000000
VERSION     DATCXU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219913"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIR04_01165"
     CDS             complement(<1..224)
                     /locus_tag="VIR04_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY9459388.1"
                     /translation="MLGRIALDLTRDANQKRRIQAAIGLALQHKAELIGICTDPPTPQ
                     YMYDEAGIPEQVLTMLLGRIAEEKSETRQF"
     gene            complement(339..902)
                     /locus_tag="VIR04_01170"
     CDS             complement(339..902)
                     /locus_tag="VIR04_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY9459389.1"
                     /translation="MTRSFTLSRRQFLRYSADIALAGAGASFASGAFASAAQTCSLSL
                     DNTHTQEKISLIYSAAGQYMPAALDRLNHFLRDHYTGTVGKMDPQLFDVLHRITLTLN
                     TRQSFQVISGYRCAATNDRLRTTRGGGVARHSLHMDGKAIDVRIPGVPLAELHDAALS
                     LKAGGVGFYPRDQFVHVDTGKVRRWAG"
     gene            complement(899..>2161)
                     /locus_tag="VIR04_01175"
     CDS             complement(899..>2161)
                     /locus_tag="VIR04_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY9459390.1"
                     /translation="HFKAPASTPFDVDTYLAGALAANRLPEAVRLAEPGIPLYARLRR
                     ALAQYRELAGNPAWQARLPALPGRKLATGQAYAGIAVLAQRLIALGDLPAGTTIPAAY
                     EGALVEGVRAFQLRHGLQADGVLGEATFRQLKITPVQRARQIELTLERLRWTPLFMAP
                     RMIVVNIPEFVLRAYEVKDGKIDVQVQMKIIVGRALDTRTPVFDEDMRFIEFSPYWNV
                     PRSIVRSETIPRLRRDPAYFQQQGFEFVTKEGEPITTLSQANIEALERGELRIRQRPG
                     PHNALGDIKFIFPNNQNIYLHHTPAPELFNRYRRDFSHGCIRIEDPVALAKFVLHEEP
                     EWTEARIRDAMTNGVSKTIRLRQPVPVLITYSTVIVKNDKVYFFPDLYGQDALLDKAL
                     RQRVRTGPLSAHTPQARETRPLESRQLP"
BASE COUNT          458 a          598 c          622 g          483 t
ORIGIN      
        1 aaaaattgcc gggtctcgga tttttcttct gcgatccggc caagcagcat cgtcagaacc
       61 tgttcgggaa tgcccgcttc gtcgtacata tattgcggcg ttggcgggtc agtgcaaatt
      121 ccgatcagct cagccttgtg ctgtaacgca agtccgatgg cagcctgtat gcggcgtttc
      181 tgattggcgt ctctggtcag atccagtgcg atgcgtccga gcatgtcaac ctcctggagg
      241 atgattggtg aatgtttggc cagtctataa ccacacccac ccggaatact tgatatgcat
      301 caatatagac gttgtctgcc gcgctcggac gttttatttt agccggccca gcgtcgcacc
      361 ttgcctgtgt cgacatgcac aaactggtct cgtgggtaaa agccgacgcc tcccgcttta
      421 agcgacagcg cggcatcgtg caactcggca agcggaacgc ctggaatgcg aacgtcgatc
      481 gcctttccat ccatatgcaa gctgtgtctg gcgacaccgc cgcccctggt ggtgcgcaat
      541 cggtcattgg ttgcagcgca gcgataccct gaaatcacct ggaacgattg ccgcgtattt
      601 agcgttaatg ttatgcggtg cagaacatcg aacaactgag gatccatttt gcccacagtg
      661 ccggtgtagt ggtcgcgcag aaagtgattt aaacgatcca gcgccgccgg catgtactgg
      721 ccagccgcag agtaaatcag ggaaatcttt tcttgcgtgt gcgtattgtc aagagacagg
      781 ctgcaagttt gcgcagcgct tgcaaaggcg cctgaagcga acgatgcgcc tgccccggct
      841 aaagcgatat cagcggaata tcgaagaaac tgacggcggg aaagagtgaa tgatcgggtc
      901 atggcaactg gcgtgattca agaggccttg tttcacgtgc ctgcggcgtg tgcgcagata
      961 gcggcccggt gcgcacacgt tgtcgcagcg ctttatcgag caaggcgtcc tgaccgtaca
     1021 gatcgggaaa aaagtaaacc ttatcatttt ttacgatcac cgtgctgtaa gtgattaata
     1081 ccggcacggg ctgacgcagg cgaatggtct tcgacactcc gttcgtcatg gcatcgcgaa
     1141 tgcgagcctc ggtccattcg ggttcttcgt gaagaacgaa ttttgccaag gcgaccggat
     1201 cttcgatgcg gatgcaccca tggctgaaat cgcgcctgta tcgattgaac agttccggcg
     1261 ccggggtgtg atgcaggtaa atattctggt tattgggaaa gataaatttt atgtcgccca
     1321 acgcattatg agggccgggt cgctggcgaa tacgaagctc gccgcgttcc agcgcctcga
     1381 tattggcttg cgacagagtt gtaatgggct ctccttcttt cgtgacgaat tcgaaccctt
     1441 gctgctggaa ataggcgggg tcgcggcgca gtcgcggtat ggtttccgac ctgacgatcg
     1501 aacggggcac attccagtag gggctaaatt caataaaccg catgtcctcg tcgaataccg
     1561 gcgtccgcgt gtccagcgcg cggcccacga taattttcat ttgcacctgc acatcaatct
     1621 tgccgtcttt cacttcgtag gcccgcagta caaattccgg aatattgacg acgatcatgc
     1681 gcggggccat aaacaatggc gtccagcgca agcgctcaag ggtcagctcg atttgccgcg
     1741 cccgctggac gggggtgatt ttaagttgcc tgaacgtggc ctcgcccagg acgccatcgg
     1801 cttgcaggcc atgacgcagt tgaaacgccc gcacgccctc gaccaaagcg ccttcgtaag
     1861 ctgccgggat agtcgtgccg gcaggcagat cgcccaatgc aatcagccgc tgcgcaagca
     1921 ctgcaattcc cgcataggct tgtcctgttg ccaattttct tccgggcagc gccggcaatc
     1981 gggcctgcca ggccggattg ccggcgagtt cgcggtactg cgccagcgca cgtcgcaagc
     2041 gcgcatacag cggtatgccg ggttccgcca ggcggacagc ctccggcagc cgatttgccg
     2101 ccagtgcgcc tgccaaatat gtgtcaacat caaaaggtgt ggacgccggc gctttaaaat
     2161 g
//
LOCUS       DATCXU010000055         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1164181,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000055 DATCXU010000000
VERSION     DATCXU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164181"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VIR04_01180"
     CDS             <1..1115
                     /locus_tag="VIR04_01180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9459391.1"
                     /translation="DLGADVIKLESPSGDDMRSVGSKRHPGMAAIFLHLNRNKRSIAL
                     DLKTGQGRLACLELAKTVDVLLYNVRPQSMKRLGLDYAQFAQINPKLVYVGAYGFGSR
                     GPYAGKPAYDDLIQGMAAIPSLYEQASGDVPRYVPLAFADRMVGLHVAIAILAGVIQA
                     RKSGQGQEIEVPMFESVAQLVLGDHLGGYTFDPPEGPVGYPRLVAAQRRPYATTNGHI
                     SLLIYNDRQWSRFFQLIGRPELDRDPRFASHTARGEHISEVYGLVADVIAGNTSEYWL
                     KALEEADIPVAPLYSLDQLIQDPHLKAVGQIVSMHHPSEGAVRTVSPVGNYSKTPAAI
                     RRHAPRLGEHTREVLLEAGFGEDEIAALLKSGAAIG"
     gene            complement(1105..>2446)
                     /gene="lnt"
                     /locus_tag="VIR04_01185"
     CDS             complement(1105..>2446)
                     /gene="lnt"
                     /locus_tag="VIR04_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY9459392.1"
                     /translation="AVVALAAFEALFITLACAVSRALSARHLHVITNYRWHLLIAGVW
                     ASCWTGCEWLRGTLLTGFPWLNVGYAHVEGVLSGWAPVIGVYGIAWLASFAAGAIALL
                     ACANNTRNDAPAAIGVVVAVAAGLLGIVLGHVGWSTPSGNPLVVRLVQGNIPQSEKFD
                     PRQMLQGIDTYMHLAALPAKGDDKPPDVIVLPETVMPLFQDRYSPEIWRQWLDIAQER
                     NAVVIMGIPLHRTVNGVQRYTNGAIAFSAKSTIPELLAASMPMRYDKHHLVPFGEFIP
                     TGFRWFVDAMNIPLGDFDRGPVRQRLFDIDGQALAVDICYEDLFGEEIIQSVKPDAEH
                     GEGASILVNISNLAWFGDSWALRQHLQISRMRALETARPMIRATNTGTTAAIDPNGAV
                     RAALPVLKRGVLDVEVQGTSGLTPYVRWGNRPILIWSILLLLLGFLLRKKQPNQ"
BASE COUNT          504 a          681 c          757 g          504 t
ORIGIN      
        1 gcgacttggg ggccgatgtt attaaactgg aatccccgtc cggagacgat atgcgctcag
       61 tcggatcgaa gcgccatccc ggcatggccg ccattttttt gcatttgaat cgcaacaagc
      121 gatcgatagc gcttgatctg aaaaccgggc aaggacgtct ggcgtgcctc gaactggcca
      181 aaacagtcga tgtcctgctt tataacgtgc gcccgcaatc catgaaacga ttgggtcttg
      241 attacgccca gttcgcgcaa atcaatccaa agctggtcta tgttggcgcc tatggtttcg
      301 gcagccgcgg cccatacgcg ggcaaaccgg cctatgacga cttgatccag ggaatggcgg
      361 ccattccttc cttgtatgag caggcgtcgg gcgatgttcc ccgatatgtg cctttggcat
      421 tcgccgaccg gatggtggga ttgcatgtcg ctatcgcgat tttggcgggc gttattcagg
      481 cgcgcaagag cgggcagggg caagagatcg aagtgcccat gtttgagagc gtggcgcagc
      541 tcgtgctggg cgaccatttg ggcggttata cgttcgatcc tccggaaggg cctgtgggtt
      601 atccccgttt ggtggccgcg caacgccggc cttatgccac cacgaatggt catatcagtt
      661 tgttgattta taacgacagg caatggtcca ggttttttca gctaatcggg cgccccgaac
      721 ttgatcgcga tccgcgtttt gccagccata cggcgcgcgg tgaacatatc tcggaagtat
      781 acgggctggt ggcggacgtc atcgccggca ataccagcga gtactggcta aaggcgcttg
      841 aggaggccga tattcctgtg gcgccgttat atagtctgga tcagctcatc caggacccgc
      901 acctgaaggc tgtcggtcag atagtaagta tgcatcatcc cagcgaaggg gccgttcgca
      961 ccgtttcccc ggtgggcaat tattcgaaaa cgccagcggc catccggcgt catgctcctc
     1021 gacttggcga gcatacgcgc gaagttttgc ttgaggcggg tttcggcgag gatgagatcg
     1081 ccgcgctgct caagtcaggg gccgctattg gttaggctgc tttttccgca acagaaaacc
     1141 caatagcagg aggaggatgc tccagataag gattggacgg ttgccccagc ggacataggg
     1201 tgtcagaccg cttgtgcctt ggacttcgac atcaagcacg cctcttttca agacggggag
     1261 cgctgcgcgc acggcgccgt tggggtcgat cgcggcagtc gtgccggtgt ttgtggcgcg
     1321 tatcatcggg cgcgccgttt caagtgctcg catgcgtgaa atctgcagat gctggcgcag
     1381 ggcccaggag tcgccgaacc aggccagatt gctgatattg accagtatcg acgcgccctc
     1441 gccgtgctcg gcgtcgggct tgaccgactg aatgatttct tcgccaaaca ggtcttcgta
     1501 gcaaatatcg acggcgaggg cctgtccatc aatgtcgaac agtcgctggc gcaccggtcc
     1561 gcggtcaaaa tcgcccaatg ggatattcat tgcgtcgacg aaccatcgga atcccgtcgg
     1621 tatgaattcc ccgaatggaa ccagatggtg tttgtcgtaa cgcattggca tgctggcggc
     1681 gagcagttca gggatagttg attttgccga aaaggcgatg gcgccattgg tgtatcgctg
     1741 aactccgttt acggtccggt gcagcgggat acccatgatg acgaccgcat tgcgctcctg
     1801 cgcaatatcg agccattgcc gccatatttc cggcgagtag cggtcctgga acagcggcat
     1861 gaccgtttca ggaaggacga tcacgtcggg cggcttatcg tcgcctttgg ccggcagcgc
     1921 ggcaagatgc atgtaggtgt cgatcccttg cagcatctgt cgcggatcga atttttcgga
     1981 ttggggtatg ttgccttgca caaggcggac aacgagcgga ttgcccgaag gcgttgacca
     2041 tccgacatgg cccagaacga tgcccagcaa accggcggca acggcgacga ccacgcctat
     2101 tgccgcgggc gcgtcgttgc gtgtgttgtt ggcgcaagcc agcagggcaa ttgcgccagc
     2161 ggcaaatgaa gcgagccagg cgatgccgta cacgccaata acaggagccc agcccgaaag
     2221 cacgccctcg acatgcgcat agccgacgtt cagccaggga aagccggtaa gcagcgtgcc
     2281 acgcagccat tcgcagcccg tccagcacga agcccagacg ccggcaatca gcaagtgcca
     2341 gcggtagttc gtgatcacat gcagatggcg tgccgaaagc gcgcgcgaca ccgcgcatgc
     2401 caaggtaatg aacaaggctt cgaacgcggc cagcgcgacg acggca
//
LOCUS       DATCXU010000056         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1082834,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000056 DATCXU010000000
VERSION     DATCXU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082834"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VIR04_01190"
     CDS             complement(<1..371)
                     /locus_tag="VIR04_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY9459393.1"
                     /translation="MTQGIYLTQDEQRVTRAQIRELNAGEFPDHDVTVAVEYSTLNYK
                     DALAITGKGRVVRRFPIVPGIDLAGTISASRSPNWKVGDKVIVNGWGLGEEHWGGLAQ
                     QARVDSGWLTALPATMTTKEA"
     gene            complement(423..1532)
                     /locus_tag="VIR04_01195"
     CDS             complement(423..1532)
                     /locus_tag="VIR04_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEY9459394.1"
                     /translation="MTVFLNVAAASANAAAPSGTGQRTTLADLSPAYFGMVMATGIVS
                     LAAHFLAHEILARVLFYLNIGVYGVLWVMTVVRALRYPRRFFGDMIDHLRACGFFTIV
                     AGTSILGSQAIILSGDHDAGFALWCLAVALWLGLTYTIFAAFTIKMDKPGLDRGINGG
                     WLLAVVATQSIAVLSSLLAAKSGMPYKLEMNFFALSMWLWGGMLYIWTMSLIFYRYTF
                     FRLSPGDLAPPYWINMGAMAISTLAGCLLINDAPGAPYLMSLVPFLKGFTIFYWATGT
                     WWIPMLLVLGLWRYVYKRFPFRYDPLYWGAVFPLGMYTACTFQLNVAMQFEFLSSIAK
                     VFFPIALIAWAATFLGLVVQLCGRALHRATVTEPL"
     gene            complement(1541..2527)
                     /locus_tag="VIR04_01200"
     CDS             complement(1541..2527)
                     /locus_tag="VIR04_01200"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HEY9459395.1"
                     /translation="MASKIKIAIAGYDNLGRGVEQAVSRNGDMELTGIFTRRDPEKLI
                     PCNAGTPVRALDSIKEFTGAIDVLILCGGSKSDLPEQGPALAAMFNTVDSFDTHPKIP
                     DYFDAVNRSAQKGGTTAIISAGWDPGLFSLNRLYGEAILPDAATYTFWGKGLSQGHSD
                     AIRRIEGVKAAVQYTIPSPDAVQRARSGERPALSTREKHTRDCYVVLKSGADAEKIRR
                     AIVTMPDYFADYDTSVTFISDDELQKNHSAMPHGGVVIRTGETGTGARQVMEYSLKLE
                     SNPEFTASVLTACARAAFRLNQQGQVGAKTVFDIAPGLLSPKSAAMLRKELL"
     gene            complement(2527..3177)
                     /locus_tag="VIR04_01205"
     CDS             complement(2527..3177)
                     /locus_tag="VIR04_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY9459396.1"
                     /translation="MTTRLKIDFVSDVSCPWCAVGLSSLESALTRLGDQVDADLHFQP
                     FELNPDMPPEGKDIVEYMTQKYGYTPQRIKSNAEAIRVRGEQVGFTFHMDKRTRSYNT
                     FDAHRLLHWAEIQGRQRALKHALFKAYFTDGEDPSSRAVLIQVAQQAGLDVEQAKKIL
                     SSDAYATEVRAREQFYLDQGIHSVPAVIVNDRHLIQGGQPPEVFEQALLQIAAETV"
     gene            complement(3246..4190)
                     /locus_tag="VIR04_01210"
     CDS             complement(3246..4190)
                     /locus_tag="VIR04_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9459397.1"
                     /translation="MNYTPGRLVAYGCLALSMSLVGSYVALSKPLAAALPVFLLAWLR
                     FGIGGLAMLRWIRKPTSEPSMSWQTRKLVFFESFLGNFLFTLCMIYGVTLTSAVSAGV
                     IMSTIPAMIALMSWFFLKERISARLWAAVACGALGIALVALAKGDSASDPLMAQGGTH
                     DKVWLGNLLIFGTVLCEGSYAVIGKKLTAVLSPKRISAVINLWGFVLTTPMGVYLALD
                     FDFAAVDARIWMLLLFYGLAASVWTVWLWMTGLKSVPASQAGVFTVMLPVSTALTGIL
                     ALGETLTLIHAISFVIALAGLLLATVHVPVPKAGRRTL"
     gene            4296..>4578
                     /locus_tag="VIR04_01215"
     CDS             4296..>4578
                     /locus_tag="VIR04_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249839.1"
                     /note="oxidative demethylase of N1-methyladenine or
                     N3-methylcytosine DNA lesions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY9459398.1"
                     /translation="MNLNLFDSEPGENWPDRLGQHAVVLRGCALASVAELLPALEAIL
                     RVAPFRYMVTRGGFRMSVALTNCGTLGWTSDRHGYRYCRSDPESGLDWPA"
BASE COUNT         1129 a         1338 c         1202 g          909 t
ORIGIN      
        1 atcgcctctt tagtggtcat ggtggccgga agcgccgtca gccatccgct gtcaactcgg
       61 gcttgttgcg ccaggccgcc ccagtgctcc tctcccaaac cccagccatt gacaatgacc
      121 ttgtcgccca ccttccagtt cggcgaacgg ctggcactaa ttgtgcccgc caggtcaatg
      181 ccaggcacaa tggggaatcg ccttaccacg cggcctttgc ccgttatagc cagtgcgtcc
      241 ttgtaattca gcgttgagta ttcgaccgcc accgtcacat cgtgatcggg aaactcgcct
      301 gcattaagct cgcggatttg cgcgcgagta acgcgctgct cgtcttgtgt taaataaatg
      361 ccctgtgtca cgtatagctc catttatcca aagttaaaac agtggctccc gaataaagcg
      421 gactaaagcg gctcggttac ggtagcgcga tgcagcgcgc ggccgcacag ttgcacgaca
      481 agccccagaa aagttgccgc ccaggcgatc agcgcaatgg ggaaaaaaac cttcgcgatg
      541 gacgacagaa actcgaactg catggctaca ttcagttgaa aggtgcatgc ggtatacatc
      601 cccaacggaa aaaccgcgcc ccagtaaagc gggtcatatc gaaagggaaa gcgcttgtat
      661 acgtagcgcc acagccccag caccagcaac atgggtatcc accacgtgcc tgtggcccaa
      721 taaaagatgg taaacccctt caggaagggg accagcgaca tcaggtaagg cgcgccgggc
      781 gcatcgttga tcagcaggca gccggcaaga gtcgagatcg ccattgcccc catgtttatc
      841 cagtaaggcg gcgccagatc gccgggcgaa aggcgaaaaa acgtatagcg gtaaaaaatc
      901 agcgacatcg tccaaatgta gagcataccg ccccaaagcc acatggaaag cgcgaaaaaa
      961 ttcatttcca gcttgtaagg cattccgctt ttggccgcca ataacgagct taggacagca
     1021 atcgattgcg tggccaccac agccagcaac cagccgccgt taatgccccg gtcgagaccc
     1081 ggcttgtcca ttttgatggt aaaggcggca aaaatcgtat aggtcagacc cagccacagc
     1141 gctacggcca ggcaccagag cgcaaacccc gcgtcgtgat cgcctgaaag aatgatggcc
     1201 tggctgccta gaatgctggt gccggccacg attgtgaaaa agccgcaggc ccgcagatgg
     1261 tcgatcatgt cgccaaaaaa acggcgtggg tagcgcagcg cgcgaacgac tgtcatgacc
     1321 caaagcacgc cataaacacc gatattaaga tagaacagga cgcgcgcaag gatttcatgc
     1381 gccaagaaat gggcggccag cgaaacaatg ccggttgcca tcaccatacc aaaataggcg
     1441 ggcgataaat cggccaaagt ggtcctttgc ccggttcctg aaggcgccgc ggcattagcc
     1501 gaagcggcag caacgttaag aaagaccgtc atggcatgcg ttaaagcaat tctttgcgca
     1561 gcatcgccgc gctctttggc gaaagcaaac ccggcgcaat atcgaacacc gtttttgcgc
     1621 ccacctgacc ttgctgatta agccgaaaag cggcgcgcgc gcaggccgtc aaaacactgg
     1681 ctgtgaattc aggattgctt tcgagcttta acgaatactc cataacctgc ctagcgccgg
     1741 ttcccgtttc ccccgtacgt ataaccacac ctccatgggg catggcggaa tgattctttt
     1801 gcaactcatc gtcactgata aacgttacgc tggtgtcgta atcggcaaaa taatcgggca
     1861 tggtaacgat ggcccgcctg atcttttccg catcagctcc cgacttaagg acaacataac
     1921 agtcgcgcgt gtgtttttcg cgcgtcgaca gtgccggacg ttcaccgcta cgcgcgcgtt
     1981 gcaccgcgtc gggcgaagga atggtgtatt gcacggccgc tttgacacct tcgattcttc
     2041 ttattgcgtc ggagtggccc tgactaagcc ccttgcccca gaaagtatac gtcgccgcgt
     2101 ccggcaatat ggcttctcca taaagacggt taagcgaaaa caaacccgga tcccaacctg
     2161 ccgagataat cgcagtggtg ccgccttttt gagccgaacg gttcaccgca tcgaaataat
     2221 ccgggatttt tggatgggta tcgaaactat ccaccgtgtt gaacatcgcc gccaaggccg
     2281 ggccctgttc aggcaagtcg ctttttgaac cgccgcaaag aataaggaca tcaatagcgc
     2341 cggtaaattc ctttatggag tcaagcgccc gaaccggcgt cccggcgttg catgggatta
     2401 acttttccgg gtctcttcgg gtgaatattc ccgtcaattc catatcgcca tttcttgaaa
     2461 cagcctgttc aacaccgcgg ccaaggttgt cgtaacccgc aatggcaatt tttatttttg
     2521 acgccattac acggtctcgg cggcaatttg cagcaacgcc tgttcaaaaa cctcgggcgg
     2581 ctgccctccc tgaattaagt ggcgatcatt gacaatgacc gccggcacgg aatggatgcc
     2641 ctgatcaaga tagaattgtt cgcgcgcccg cacttccgta gcgtaagcgt ccgacgacaa
     2701 gatttttttc gcctgctcga cgtcaagacc cgcctgttgc gccacctgga ttaaaaccgc
     2761 gcgtgagctg ggatcttcgc catccgtaaa ataggccttg aacaaggcat gcttgagggc
     2821 gcgttggcgc ccttggattt cggcccagtg caacaggcga tgcgcatcga acgtgttata
     2881 ggagcgggtc cgtttgtcca tatgaaaggt gaacccgact tgttcgcccc ggacccggat
     2941 ggcctcggcg ttgctcttga tacgctgcgg cgtatagccg tatttttgcg tcatgtattc
     3001 gacgatatcc ttgccttcgg gcggcatatc cggattcagc tcaaacggct gaaaatgcag
     3061 gtccgcgtca acctgatccc cgagccgggt caaggcgctt tcaagcgaac ttaaaccgac
     3121 cgcgcaccag ggacacgaaa cgtcggaaac aaaatctatt ttgagacgag tggtcatttc
     3181 aactccgtgg tcaagtgatg cataaaggaa ttaaagcacc gccgggtacg cccgacaatt
     3241 ttagcctaaa gtgtacgccg tccagccttc ggaaccggca catggacggt ggccagcaac
     3301 agaccggcca aagcaatgac gaatgaaatc gcatgtatga gcgttagcgt ttcacccagc
     3361 gccaaaatgc ccgtcaatgc ggtgctgaca ggcaacatca ctgtaaacac ccccgcctgc
     3421 gaggcaggaa cagatttcaa cccggtcatc cacaaccaga cagtccacac gctggctgcc
     3481 aggccataaa aaagcaacag catccagatc cgcgcgtcga ctgccgcgaa atcaaaatcc
     3541 agagcaagat aaacgcccat cggcgtggtg agcacgaaac cccacagatt gatgactgcg
     3601 ctaatccgct tcgggcttag aacggctgtc agctttttgc caatgacggc gtaagagcct
     3661 tcgcacaaaa ccgttccaaa gatcagaaga ttgcccagcc acaccttatc gtgcgtcccc
     3721 ccttgcgcca tcaacgggtc cgacgcgcta tcgccctttg ccagcgccac cagggctatg
     3781 cccagtgcgc cacaagccac tgccgcccac aaacgcgcac taatgcgttc ttttaaaaaa
     3841 aaccagctca ttaacgcaat catggcgggt atcgtcgaca taatgacgcc ggcggacacg
     3901 gcactggtca aggtcacgcc atagatcatg caaagggtaa aaaggaaatt acccagaaac
     3961 gactcgaaaa acaccagctt gcgcgtctgc cacgacatgg acggttcaga cgtcggcttg
     4021 cgtatccagc gcagcattgc caatccgcca atgccgaacc gcagccacgc cagcaaaaaa
     4081 acgggaagcg ccgccgccag cggcttggac agagccacat aactacccac caatgacatg
     4141 ctcagcgcca ggcagccgta ggccaccagt cggcccgggg tgtagttcat tgaatcactt
     4201 tagtaaatgc cgaattgcct gaagcaggac ctgttgaatt gccgtgaata tgacatatta
     4261 ttagcagtgt tgaaaggaaa actaagcggc gccggatgaa tctgaacctt tttgacagcg
     4321 agcctggcga aaattggccg gatcgattgg gtcaacacgc cgtcgtattg cgcggttgcg
     4381 cgctggccag cgtggcggaa ctcctgcccg ctctggaggc gattttgcgc gtcgcgccct
     4441 tccggtacat ggtcacgcgc ggcggttttc ggatgtcggt tgcgctgaca aattgcggca
     4501 cgcttggatg gaccagcgat cgccacggtt accgctattg ccgtagcgac cctgaaagcg
     4561 gtcttgactg gcctgcca
//
LOCUS       DATCXU010000057         5450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_219396,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000057 DATCXU010000000
VERSION     DATCXU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219396"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VIR04_01220"
     CDS             <1..590
                     /locus_tag="VIR04_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY9459399.1"
                     /translation="GLPAAMGMALLSDGLVATLFNYGAFTGHDVMQTRLAVMAYSVGL
                     LGLLSIKILAPGFYAKQDIRTPVKIAIFVLILTQVFNVIFVPLFAHAGLALSIGLGAS
                     VNALFLLLMLYKHKIYQPRPGWGRFVLKVAPALAALALVLSMAGAYIDWVGLGTRPWL
                     RVLWLGGVLLASAAAYFGVLLLCGFRPRDFTRRSA"
     gene            686..949
                     /gene="rpsT"
                     /locus_tag="VIR04_01225"
     CDS             686..949
                     /gene="rpsT"
                     /locus_tag="VIR04_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY9459400.1"
                     /translation="MANTAQARKRARQSVDRNKQNASLRSALRTSIKRVREAIAAGDK
                     SAASEVFQKTTSVIDRIADKNIIHKNKAARHKSRLAAAIKALA"
     gene            1089..2054
                     /locus_tag="VIR04_01230"
     CDS             1089..2054
                     /locus_tag="VIR04_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY9459401.1"
                     /translation="MPQQKPTLLQLRPLTVLDDGQARLNRVFNVIELWKEANPDETIL
                     AHKNDAVVLMTSGIAGANAELIDKLPKLQAICHWGVGYDSIDVKHAQQKNIQISNTPG
                     VLNDCVADLTWGLILATARRMGQNERYARASLWGKEPARPLGTRVSGKNLGIVGMGRV
                     GLAIADRSVGFDMKVRYHNRHARPDTHWAYESSLIEIAKWADFLVVVTVGGPSTYHLI
                     DKSVINALGPDGMLINVARGSVVDQDALIKALANGELGAAGLDVYEDEPSIPDELKSM
                     DNVVLLPHIGSATTETRRAMSNLAVENVEAFAATGKVITPIDKAA"
     gene            complement(2065..2391)
                     /locus_tag="VIR04_01235"
     CDS             complement(2065..2391)
                     /locus_tag="VIR04_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY9459402.1"
                     /translation="MTPIVQQFIIHGTTQEGKRFRPSDWAERLAGVLSRFRPAGSFGG
                     PLTYSPYATPLLLNGEVCVAIDHRLRSLEPLAWKFACEFAASNQLCTSERELSAPGQA
                     RLPQET"
     gene            2556..3497
                     /gene="argF"
                     /locus_tag="VIR04_01240"
     CDS             2556..3497
                     /gene="argF"
                     /locus_tag="VIR04_01240"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY9459403.1"
                     /translation="MSSATPQVGPLRHFLQFRDFTKDEIIYVLNRARLIKDKFKRYEA
                     HHTLHDRNLAMVFEKASTRTRVSFEAGMYQLGGSVIHLTTADSQLGRAEPIEDTARVI
                     SRMVDVVMIRTFEQTRIERFAAHSRVPVINGLTNEFHPCQILADIFTFIEHLGSIEGK
                     TVAWIGDANNMSYTWLQAAEILGFRLHVSGPKGYLLDRERIGQPPASVLQEFADPMDA
                     CRGAHLVTTDVWTSMGYEDENEERRAAFADWCVDSDMMAVADPKAIFMHCLPAHRGEE
                     VTGDVIDGPQSVVWDEAENRLHVQKALMEFLLLGKVD"
     gene            3615..4835
                     /locus_tag="VIR04_01245"
     CDS             3615..4835
                     /locus_tag="VIR04_01245"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY9459404.1"
                     /translation="MSEVKKVVLAYSGGLDTSVILKWLQDTYQCEVITFTADIGQGEE
                     VEPARAKALKFGIKPDHIFIDDLREEFVRDFVFPMFRANAIYEGEYLLGTSIARPLIA
                     KRQIEIARQVGANTVSHGATGKGNDQVRFELSYYSLEPGIKVIAPWREWDLTSREKLL
                     AYAQDAGIEIDRKHKQGGAPYSMDANLLHISFEGRHLEDPKSEAEDSMWRWTVSPEQA
                     PDAPEYVDVEFENGDAVGLNGKRLSPAQLLTKLNELGGKHGIGRLDLVENRSVGMKSR
                     GCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYAAMIYNGYWWSPERRALQAL
                     IDYTQRTVNGWARLKLYKGGVYTVARDSKNTLFDQKIATFDEDGGAYDQRDAAGFIKL
                     NALRLRIEARAERK"
     gene            4840..>5450
                     /gene="mnmH"
                     /locus_tag="VIR04_01250"
     CDS             4840..>5450
                     /gene="mnmH"
                     /locus_tag="VIR04_01250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY9459405.1"
                     /translation="MNTLRVTLERIHDFDEIVDVRTPLEYAEDHIPGAINAPVLSNEE
                     RVVIGTLYTQSRFEATRLGAALVAKNIGRHLETQFANRPRQWRPLIYCWRGGKRSEAM
                     STWFNLIGWQARQLEGGYKTYRRWVIEMVQSLPGRFHYIILTGPTGTGKTRLLHALKR
                     AGAQTLDLEDLARHRGSLLGALPGQSQPSQKSFDSRLVDTLLP"
BASE COUNT         1234 a         1506 c         1559 g         1151 t
ORIGIN      
        1 tgggcctgcc cgcggctatg ggcatggcgc tgctttcaga cggtctggta gccacgctgt
       61 tcaattacgg cgcttttacc ggacacgacg tcatgcagac gcgtctggcc gtaatggcct
      121 attctgtggg cctgctgggt ctgctttcaa tcaagatact ggctccggga ttttatgcca
      181 agcaagacat acgaacgccc gtcaaaatag ctatttttgt actgattctg acccaagtgt
      241 tcaacgttat tttcgtgccc ctgttcgccc atgcggggct tgccttgtcg attgggctgg
      301 gcgccagcgt taacgcattg tttttattgc ttatgctgta caagcacaag atataccagc
      361 cgcgtccagg ctggggacga ttcgtgctga aagtggcccc cgccctggct gcgctggcgc
      421 tggtattgtc aatggccggc gcatacatcg actgggtcgg actgggaact cgcccctggc
      481 tacgcgtatt gtggctgggc ggtgttctcc tggctagcgc ggcagcgtat ttcggagtgc
      541 tgttgctatg cggttttcgg ccgcgcgact ttacacggcg cagcgcctaa tgtagaatag
      601 tcgtctttcc tgaatacaaa tacgctgtgg cgccacggcc aacgcattag ttgttttcaa
      661 atttttagag aaaaaagcac caaacatggc taatactgcc caagctcgca aacgcgctcg
      721 ccagtcggtt gatcgcaaca aacaaaacgc cagtctgcgt tcagcgcttc gcacgtcaat
      781 caaacgcgtg cgcgaggcca ttgcagccgg cgacaaatcg gccgcttctg aagttttcca
      841 gaaaacgaca agtgttattg atcgcattgc cgacaagaat attattcaca agaacaaggc
      901 cgcacgccac aaaagccgcc tcgcagccgc catcaaggcg cttgcctgat cactggctta
      961 agcagcaaat aagacaccgg ccggtcaagg ctggtggcaa atgggacgcc ctggcgtccc
     1021 attttgtcgc cggggcttca aatcacttat actttgggct catttcaaca acaccatgag
     1081 acaaagcaat gccccaacaa aaacccactc tgttgcaact gcgcccattg accgttctgg
     1141 acgacggaca ggcgcgcctg aatcgtgttt ttaacgtcat cgagttgtgg aaagaggcga
     1201 accccgacga gaccattctt gcccacaaga acgacgccgt cgtactgatg accagcggaa
     1261 tagctggcgc gaatgccgag ctcatcgaca aattgcccaa gctgcaagca atttgccatt
     1321 ggggcgtggg ctacgactcg atcgacgtca aacacgcaca gcaaaaaaac atccaaatca
     1381 gcaacacgcc tggggtgctt aacgactgcg tggcggatct gacgtggggg ctgattctgg
     1441 ccaccgcccg tcgcatggga caaaatgaac gctacgcccg ggcaagtcta tggggcaaag
     1501 aaccggcgcg cccgctgggc accagggtca gcggaaagaa tctgggtatt gtcggtatgg
     1561 gccgcgtggg gttggcgatc gccgaccgtt ctgtgggctt tgatatgaag gtgcgctatc
     1621 ataatcgtca tgcgcggcct gacacgcatt gggcctatga aagctcgctg attgagatcg
     1681 caaaatgggc cgactttctc gtggtcgtta cggtgggcgg cccgtcgacc taccacctta
     1741 tcgataagtc ggttatcaac gcgctcggac ccgacggcat gctcatcaat gttgcgcgcg
     1801 gctcggtggt tgaccaggac gcacttatca aggcgctggc aaacggtgaa ttgggtgctg
     1861 ccgggcttga tgtctacgaa gacgaaccca gcataccaga tgagcttaaa agcatggata
     1921 acgtggtatt gctgccccat attggcagtg caaccacgga aacccgccgg gccatgtcca
     1981 atctggccgt ggaaaatgtc gaagcctttg ccgcaaccgg caaggtcatc acgccgattg
     2041 acaaagcggc ctgatcgcgg ctttttacgt ttcttgcggt aagcgcgcct gaccgggcgc
     2101 gcttaactcg cgctcgctcg tgcataattg attgcttgcg gcaaactcgc aggcaaactt
     2161 ccacgccagg ggctccagac tgcgcaagcg atgatcgatc gcgacacaga cctcgccatt
     2221 aagcaataag ggcgtggcat acggcgaata ggtcagtggc ccgccaaacg agcccgcagg
     2281 ccgaaaccgc gacagcactc cggcaagacg ctctgcccaa tcgctggggc gaaaccgttt
     2341 gccctcttgt gtcgtgccgt ggatgatgaa ttgctgcaca ataggtgtca tagatttccg
     2401 cagacaaact ttaccgactt cgggcgtcct aaaattttcc cgcgccgaga ttgtaattga
     2461 tcggcgcaat cgcaatgtat ccggctgatt ttcaaggccg tcttttacag atcggggccg
     2521 ggcaggtaag atgcttgttt taccttgggt tcaacatgtc ttctgccaca ccgcaagtgg
     2581 gtcccctacg gcatttcctg caatttcgtg attttacaaa agacgaaatc atttatgtgc
     2641 tgaaccgagc gcgattgatc aaagacaagt tcaaacgcta cgaggctcat catactttgc
     2701 acgatcgcaa cctggccatg gtgtttgaaa aggccagcac gcgtacgcgc gtgtcattcg
     2761 aagcgggcat gtatcagtta ggcggatcgg tgatccacct gaccaccgct gactcgcaac
     2821 tggggcgggc ggaacccatc gaggacacgg cgcgcgttat ttcgcgcatg gtcgatgtgg
     2881 tcatgatccg tactttcgaa caaacgcgta ttgaacgctt tgccgcgcac tcgcgggtgc
     2941 cggtcatcaa cggcctgacg aatgaatttc atccttgcca gattctggcg gacattttca
     3001 cgtttattga acatctgggt tctatcgaag gcaaaacggt tgcctggatc ggcgatgcga
     3061 acaacatgtc gtacacctgg ttgcaagcgg ccgagatcct gggattccgg ttgcacgttt
     3121 ccggacccaa aggctatctg ctggaccggg agcgcattgg ccagccgccg gcttcggtat
     3181 tgcaggaatt cgccgacccg atggacgcgt gccgaggcgc gcatctggtg actacggacg
     3241 tgtggacgag catgggctac gaggacgaaa acgaggagcg gcgcgccgca ttcgcggatt
     3301 ggtgcgtaga ttcggacatg atggcggtag cggatccgaa ggcgattttc atgcattgcc
     3361 tgcccgcgca tcgcggcgag gaagtcaccg gcgatgtgat cgatggaccg caaagcgtgg
     3421 tgtgggacga agccgaaaat cggctgcacg tgcaaaaggc gttgatggag tttttgctgc
     3481 tgggaaaagt ggattaactg gcgccttcac attatccgga cctcgggttc ggttaaaatg
     3541 atttgttgag cgcggtaacg ctccctgccc ttttgtctgt cgcgaagggc caacccctaa
     3601 atttgcgtat attcatgagc gaagtcaaaa aagttgtgct cgcctactcg ggcggcctgg
     3661 atacatcggt cattctgaaa tggctgcaag atacttatca atgcgaagtc atcacgttta
     3721 cagccgatat cggccagggc gaagaggtcg agccggctcg cgcgaaggca ttaaaattcg
     3781 gcattaagcc ggaccatatt tttattgatg atttgcgtga agaattcgtc cgtgacttcg
     3841 tgtttccgat gttccgcgca aacgcgatct acgaaggcga atacctgctt ggaacgtcga
     3901 ttgcacggcc gttgatcgcg aagcgccaaa ttgaaatcgc gcgtcaggtg ggcgccaaca
     3961 cggtgtcgca cggcgctacc ggcaagggca atgatcaggt gcgtttcgag cttagttatt
     4021 attcgcttga accgggcatt aaagtgatcg ctccgtggcg cgagtgggat ttgacgtcgc
     4081 gtgaaaagct gctggcgtac gcgcaagacg ccggcatcga gatcgacaga aaacacaaac
     4141 aaggcggcgc gccctattcg atggacgcaa acttgctgca tatcagcttc gaggggcgcc
     4201 atctggaaga ccccaagtcc gaagccgagg actcgatgtg gcgctggacg gtatcgcccg
     4261 agcaagcgcc cgacgccccc gaatatgtgg atgtcgaatt cgagaacggc gacgcggtcg
     4321 ggcttaacgg caaacgcctg tcgcccgcac agttgctcac caagctgaac gagctgggcg
     4381 gcaagcacgg aattggccgg ctggacctgg tcgaaaaccg ttctgtcggc atgaaatcgc
     4441 gcggttgcta cgagaccccg ggaggcacga tcctgttgcg cgcgcaccgc gccattgaat
     4501 ccatcacact ggatcgcgaa gtggcgcatc tgaaagacga tctgatgccg cgctatgcag
     4561 ccatgattta caacggctac tggtggtcgc ctgagcgcag ggctttgcaa gcgctcatcg
     4621 actatacgca acgcacggtg aatggctggg cacgcctcaa gctctataag ggcggggtct
     4681 ataccgttgc gcgcgactca aaaaacacct tgtttgatca aaaaatcgca acattcgacg
     4741 aagacggcgg cgcatacgac cagagggacg cggcgggctt catcaagttg aacgcattgc
     4801 gtttgcgcat cgaagccagg gcggaacgca aataagcgtg tgaacacttt gcgcgtcacc
     4861 cttgaacgca tccacgactt cgatgaaatc gtggatgtcc gcactccgct tgaatacgcc
     4921 gaagaccata tcccgggcgc aatcaacgct cccgttctaa gcaacgaaga gcgcgttgtc
     4981 atcggcaccc tgtacactca gtcacgcttt gaagccacgc ggctgggcgc cgcactggtc
     5041 gccaaaaaca ttggccgcca ccttgaaacg caattcgcaa atcggccccg gcaatggcgg
     5101 ccactcatct attgctggcg cggcggcaaa cgctcggaag caatgagcac ctggttcaac
     5161 ctgatcggat ggcaagcccg ccaactggaa ggcggctata aaacgtaccg ccgctgggtt
     5221 attgaaatgg tgcaaagcct gccaggccgg tttcactata ttattttgac cggtcccacc
     5281 ggaacaggca aaacccgctt gttgcatgca ttgaagcggg ctggcgcaca aacgctggat
     5341 ctggaagacc tggcacgcca tcgcggatcc ttgctgggcg ccctgccggg acaaagccag
     5401 ccctcgcaaa aaagcttcga ttcgcggctg gtcgatacac tgctgccctt
//
LOCUS       DATCXU010000058         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_693300,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000058 DATCXU010000000
VERSION     DATCXU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693300"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1079
                     /locus_tag="VIR04_01255"
     CDS             15..1079
                     /locus_tag="VIR04_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9459406.1"
                     /translation="MNPNEQKLTYPWGDALPEPGHALQVAEGVRWIRMPLPFALDHIN
                     LWLLRDEIDGRDGWTVIDCGVSRDEVKGLWERVFENALDGLPVLRVIVTHMHPDHVGL
                     AYWLCKRWDAPLWMTMTDYALARLWSQPADDDTSGPAGQAAVEHFMRHGLADPQAQAR
                     IRGRSNYYSNLVPDVPKHFRRMMKDDRIVIGGRSWRVFIGYGHAPEHASLYCADLRVL
                     VAGDMVLPRISTNISVFNFEPESNPLPLYLNSLDDYDGLPEDTLVLPSHGRPFKGLHE
                     RIAQQHAHHADRLADVMQACVKPCSTADIVPVLFKRKLDFHQLTFAMGEALAHLHALW
                     FEGKLARTMGEDGIIRFCVK"
     gene            1097..2296
                     /gene="metC"
                     /locus_tag="VIR04_01260"
     CDS             1097..2296
                     /gene="metC"
                     /locus_tag="VIR04_01260"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY9459407.1"
                     /translation="MPNSASFSRLDTLLQHAGMAAFNSETGAAPVAVPSMRTSTVRFS
                     DLDALDRAQASKGTGERAVTYGRIGMDTHAALEQVFCDLEGAQRAFLAPSGLAAVTLA
                     LFSLLSSGDHLLVADCAYGPIRYLDKIVLKRMNIQVSYCRATPADLASQLRPNTRVLY
                     VESPGSLLFEMLDIPALARFAKQHSLILVTDNTWGSGYIYRPLDLGADVSVVAGTKYV
                     GGHSDLMLGSVMANDPALIRTINDTHYAMGYSISADDAWLALRGVRTLPIRMRQHAGH
                     ALRICEFLAGRPEVARIYHPALPTDPGHALWLRDCTGSNGMLSVELRLDEAAARRFVN
                     ALVLFGIGYSWGGFESLVELVDHAALACHSYWTASENAVVRFHIGLESPSDLIADLTQ
                     ALEAARA"
     gene            complement(2297..2935)
                     /locus_tag="VIR04_01265"
     CDS             complement(2297..2935)
                     /locus_tag="VIR04_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY9459408.1"
                     /translation="MSLNKLFLRAFAVIAIGSGALFMPAVQAIAASAGPYTTVSPAQP
                     SDTQGKIEVLEFFAYTCPHCNAIEPLVEKWTKTLPANVVFRPVPVAFNASMADLQKLY
                     YSLEAMGRLDLHPAVFKAIHEEHKQIFDAKAIIDWVAAQGVDRDQFKAVFNSFGINSK
                     VARANELAKNYKIEGTPSLAIGGEYVTSPSETKSYAGTIEEAQKLLEMVAKK"
     gene            complement(3016..>3351)
                     /locus_tag="VIR04_01270"
     CDS             complement(3016..>3351)
                     /locus_tag="VIR04_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY9459409.1"
                     /translation="PGTGAAVPHPKAVPGTGVAIAQPKSTPAAPPAASAQKTYYLQAG
                     AFRSEKDAEATKARIIMLGLQAQVQKAQLNGTTINRVRVGPFKGIDEMNKSRVRLGEE
                     KIDSSVMRQ"
BASE COUNT          617 a          922 c         1017 g          795 t
ORIGIN      
        1 agcacggaga tttaatgaac ccaaatgaac aaaagctgac atatccatgg ggcgacgcct
       61 tgcccgagcc gggacacgcc ctgcaggtgg ccgagggcgt gcgctggatt cgtatgcctt
      121 tgcctttcgc tctggatcac atcaatttgt ggctgttgcg cgatgaaatc gacggacgcg
      181 acggatggac ggtgattgac tgcggagtct cgcgcgacga agttaaaggc ctttgggagc
      241 gtgtttttga aaacgccctg gacggcttgc cggtcttgcg cgttattgtg acgcatatgc
      301 accccgatca cgtcggattg gcatactggt tgtgcaagcg ttgggatgcg ccattatgga
      361 tgacaatgac cgactatgcg ctggcgcgtt tgtggtctca gcccgccgac gatgatacaa
      421 gcggcccggc gggacaggcg gcggttgagc acttcatgcg tcacgggttg gccgatccgc
      481 aagcccaggc gcgaattcgc ggccgatcca attattattc caatctggtg cctgacgtcc
      541 cgaagcattt tcggcgcatg atgaaagatg accggatagt gattggcggg cgctcatggc
      601 gcgtttttat cggatatggc cacgcgcccg aacatgcatc gttgtattgc gccgacctcc
      661 gggtcctggt ggcgggcgac atggtgctgc cgcgtatttc caccaatatt agcgtgttca
      721 atttcgagcc cgagtccaat cccctgccgc tctatttgaa ttccctggat gactacgacg
      781 gcctgcccga agacaccctg gtgctgccgt cgcacggccg tccgttcaag ggcctgcacg
      841 agcgtattgc gcagcagcac gcacatcacg ccgaccgcct tgccgacgtg atgcaggcgt
      901 gcgtcaaacc ctgcagcact gcggacatcg ttcccgtcct gtttaagcgc aagctggatt
      961 tccatcagct tacctttgcc atgggcgagg ctttggccca tttgcacgcg ttatggttcg
     1021 aggggaagct tgcgcgcacg atgggcgaag acggaatcat tcgtttttgt gttaaataaa
     1081 tttcgtgaaa ttcaccatgc ctaactccgc atctttctcc cgcctggaca cattattgca
     1141 acatgctgga atggcggcgt tcaattctga aactggcgcc gcgcccgtgg ccgtgccttc
     1201 catgcgcacc agcaccgtcc ggttctccga tctggatgcg ctcgatcggg ctcaggccag
     1261 caaaggaaca ggtgagcggg ctgttacgta cggccgcatc ggcatggata cgcatgcggc
     1321 gctggaacag gtattttgcg atcttgaagg ggcgcagcgc gcctttctgg ccccttcggg
     1381 cctggcggcg gtcacgctgg cgttattcag tctgttgagc agcggcgacc accttctggt
     1441 ggccgattgc gcctatgggc ccatacgtta ccttgacaag atcgttctca agcgcatgaa
     1501 tattcaggtg tcgtattgcc gggccacgcc tgccgatctg gcttctcaat tgcgccccaa
     1561 cacccgcgtt ttatacgtcg aatctcctgg gtcgctgctg tttgaaatgc tcgacattcc
     1621 cgcgctggcc cggttcgcca aacagcacag cctgatactg gttaccgaca atacgtgggg
     1681 ctcgggttat atctatcgcc cgcttgatct gggcgccgat gtgtcggtgg tggcgggcac
     1741 gaagtacgtg ggcgggcatt ccgacctgat gctgggctcc gtgatggcca atgacccggc
     1801 gctaatcagg acgatcaatg acacccacta cgcgatgggt tattcgatca gtgcggacga
     1861 tgcctggctt gccttgcgcg gcgtgcgcac cttgcctatt cgcatgcgcc agcacgccgg
     1921 gcacgccttg cgcatttgcg agtttctggc cggccggccg gaagtagccc gaatctatca
     1981 tccggcgctg cccaccgatc caggccatgc gttgtggttg cgcgattgca ccggttccaa
     2041 cggcatgctg tcagtcgagc tgaggctgga cgaggcggca gcgcgccgtt tcgtgaatgc
     2101 gctggtcctg tttggcattg ggtattcgtg gggcggtttc gaaagcctgg ttgagcttgt
     2161 cgatcatgcg gcgctggcgt gccacagtta ctggacagcc agtgaaaacg cggtcgtgcg
     2221 ttttcacatc ggtctggaat cgccgtccga cctgattgcc gatttgaccc aggcgctgga
     2281 agcggcgcgc gcctgattat tttttggcga ccatctcaag cagcttctga gcttcttcaa
     2341 tggttccggc gtagcttttt gtttctgaag gcgatgtcac atactcgccg cctatggcca
     2401 ggctgggcgt gccttcgatc ttgtagtttt tggcaagctc attggcgcgc gcgaccttcg
     2461 aattaatccc gaaggagttg aacacggcct tgaactggtc gcggtctaca ccttgagcgg
     2521 cgacccagtc gatgatggcc ttggcatcaa aaatttgttt gtgctcttcg tgtatggctt
     2581 taaacacggc cgggtgcaaa tcaaggcgcc ccatggcttc gagcgagtaa taaagctttt
     2641 gcagatcggc catgctggca ttgaaggcga ccggaacagg gcggaacacg acatttgcgg
     2701 gtagcgtttt ggtccatttt tcgaccagcg gttcaatcgc attgcagtgc gggcaggtat
     2761 aggcaaagaa ctccaggact tctattttcc cctgggtatc cgagggttgc gcggggctaa
     2821 cggtggtgta cgggccggcg ctggcggcga tggcctgaac ggccggcata aaaagcgccc
     2881 ccgaaccgat cgcaatgacg gcgaaggcgc gtaaaaacaa tttattaagc gacatagtcg
     2941 gattccttga gttggctttt gggttgaatc gaaaataaga ccgtaaataa ggtggcgggt
     3001 tccgataccc gcttgttatt ggcgcataac ggacgaatca attttttctt cgcccaggcg
     3061 cacacgcgat ttattcattt catcgatgcc tttaaatggc ccgacgcgca cgcgattgat
     3121 ggtcgtcccg ttgagttggg ctttttgcac ttgcgcctgc aatcccagca tgataatgcg
     3181 cgctttggtg gcttcggcat ccttttcgga gcgaaatgcg cctgcctgca gatagtaggt
     3241 tttttgcgcg ctggcggcgg gcggggcggc aggcgtggat tttggttgag caatcgcgac
     3301 gcccgtgcct ggcactgctt tgggatgagg gactgcggca cctgtgccgg g
//
LOCUS       DATCXU010000059         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1163098,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000059 DATCXU010000000
VERSION     DATCXU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163098"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..709
                     /locus_tag="VIR04_01275"
     CDS             269..709
                     /locus_tag="VIR04_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459410.1"
                     /translation="MSHSILVTLHLFAAFIFVGTVFFEVLILEGVRKYVPHEVMRQME
                     LGIGQRARRIMPWAVLVLYGAGIAMAWQYRAELAHPFATTFGTLLTIKIALALSVLAH
                     FVTAIKLSGTGKLKSRHFRYIHLSVFAHMIGIVFLAKAMFFLHW"
     gene            957..>3168
                     /locus_tag="VIR04_01280"
     CDS             957..>3168
                     /locus_tag="VIR04_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY9459411.1"
                     /translation="MQTTHAAKAPPEATAPPSAATKRDANSDSQWQNYHIIRRNGAVV
                     GFEPGKMAIALTKAFLAVNGGQGAASARVRELVENLTTQAVKALIRNRPSGGTFHIEE
                     IQDQVELALMRSGEHEVARAYVLYREKRSQERARAGQAAAPTPQNTLNVTDNGVRRPL
                     DLAQLRATIAEAGLNLPNVIDADAILAETVKNLYDGIPVDEVFKSAILSARALVETDP
                     SYSVVTARLLLHTIRKEVLGEEVSQAAMATRYAEYFPQFIKLGVEGGLLDRKLAQFDL
                     PTLANALNAQRDQQFNYLGLQTLYDRYFLHLHNRRIELPQIFFMRVAMGLALNEIDRE
                     ARAIEFYELLSSFDFMSSTPTLFNSGTLHSQLSSCYLTTVSDDLAGIYDAIKENALLA
                     KYAGGLGNDWTPVRALRSYIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYL
                     ESWHLDIEEFLDLRKNTGDERRRTHDMNTANWVPDLFMKRVMEKGDWTLFSPSDVPDL
                     HDKYGRAFEQAYIGYEEKAARGELALFKTIPALSLWRKMLSMLFETGHPWITFKDPCN
                     IRSPQQHVGVVHSSNLCTEITLNTSDSEIAVCNLGSVNLAAHLKKDANGQYVLDHSKL
                     ERTITTAMRMLDNVIDINYYAVAKARNSNARHRPVGLGIMGFQDCLHRMRIPFASQAA
                     VEFSDRSTEAACYYAYWASSKLAAERGAYASFKGSLWDRGILPQDTLALLRDERG"
BASE COUNT          748 a          943 c          819 g          658 t
ORIGIN      
        1 ggccgtgata cacagccccc gatcatggcg atcgacgcca gacccgcaac gagcgtgctt
       61 ttgcgaaagg aaaaatccac cataaaaccc catcggcatc atcgagttaa gaccacaacc
      121 aacaagtggg ctcgaagcaa ggcgcgggcg tcccgctcta caatcattcc atcccacccg
      181 cgatccgggt aaccgggcgg tcattttact cgaactgccg ccaacgccgt ccgtaactat
      241 tcttgctcac gcaggagcct gcaataccat gagtcactct attctggtca cacttcactt
      301 gtttgccgct ttcatattcg tcggcaccgt atttttcgag gtattgattt tagaaggcgt
      361 acgcaaatac gtcccacacg aagtcatgcg ccaaatggaa ttgggcatcg gtcagcgggc
      421 gcgccggatc atgccctggg ccgtgctcgt tttgtatggc gcgggcatcg ccatggcgtg
      481 gcagtaccga gccgaactgg cccacccatt tgccaccacg ttcgggacat tgctcactat
      541 aaaaatcgcc ctggcgctta gcgttctggc gcatttcgtt accgccatca agctcagtgg
      601 cacaggaaag cttaaaagcc ggcactttcg ctacattcac cttagcgtgt tcgcacatat
      661 gatcggcatc gtatttttag ccaaagccat gttttttcta cactggtaat ggtaaatcgc
      721 gtttttccgg cttttcgcaa aaaaaggcta cttcagatct gaaaaaaagg gcttgccggc
      781 cttgtgagcc ggcgggttct tgtgcgggcg cggcagtaac gaaattaaaa taatttagcc
      841 cccttcgccc cttgataagc aggcattgag tcactacaat tggtgttcaa aaagagcgaa
      901 accactatat gttgtggttt catcagaaaa cacgcctctt tctaccagga gcctccatgc
      961 aaacaacaca cgccgccaag gcaccgcccg aggccaccgc accgccctcg gccgcaacaa
     1021 aacgggacgc aaacagcgat agtcaatggc aaaactacca catcatccgg cgcaacggcg
     1081 ctgtcgtcgg attcgaaccg ggcaaaatgg cgattgcgct gaccaaggcc tttctggcgg
     1141 tcaacggggg gcaaggcgcc gcctcggcgc gtgtgcgcga actggttgaa aatctgacca
     1201 cccaggccgt caaggcgctc atccgcaatc gtcccagcgg cggcacgttc catattgaag
     1261 agatacaaga tcaagtcgaa ctggcgctga tgcgttcggg cgaacacgaa gtggctcgcg
     1321 cctatgtgct ttaccgcgaa aaacgttcgc aagagcgggc acgcgccggt caagcggctg
     1381 cgccgacgcc gcaaaacacc ctgaacgtca ccgacaacgg cgtcaggcgc cctctcgacc
     1441 tggcgcaatt gcgcgcaaca atcgccgagg ccggcctgaa cctgcccaat gtgattgacg
     1501 cggatgccat tcttgcggaa accgtaaaaa acctctacga cggcattccg gtcgacgagg
     1561 tcttcaaatc ggccatcctt tcggcgcgcg ccctggtcga aacagacccg tcttatagtg
     1621 tggtgacggc gcggttgctg ctgcacacca tacgcaaaga agtattgggc gaagaggtct
     1681 cgcaagcggc catggcaacc cgctatgccg aatacttccc gcaatttata aaactgggtg
     1741 tcgaaggcgg cctgctcgac aggaagctgg cccagtttga tcttccaaca ctggccaacg
     1801 cattaaacgc tcagcgcgat cagcaattca attacctggg tttacaaaca ctgtacgatc
     1861 gctatttcct gcatctgcat aaccgccgga tcgaactgcc gcagatattt ttcatgcgcg
     1921 tggccatggg gctggcgcta aacgaaattg accgcgaagc gcgcgcgatt gaattctacg
     1981 aactcctttc gtccttcgac tttatgagtt cgaccccgac actgtttaat tcgggcaccc
     2041 tgcattctca gctttcctcg tgctatctca ccactgtctc cgatgatttg gcgggcattt
     2101 acgatgccat caaagaaaac gcactgctgg ccaagtatgc gggtggtcta ggcaacgact
     2161 ggacgcccgt gcgcgccttg cgcagctata tcaaaggcac caatggtgaa agtcagggcg
     2221 tggtgccatt tcttaaagtg gtcaatgaca ccgctgtggc ggtgaaccag ggaggcaagc
     2281 gcaaaggcgc ggtatgcgcc tatctggaaa gctggcatct ggacatcgaa gaattccttg
     2341 atctgcgtaa aaacaccggc gacgaacgac gccgcacgca cgacatgaac accgccaatt
     2401 gggttcccga tctgttcatg aagcgcgtta tggaaaaggg cgactggaca ttgttttcgc
     2461 cctccgacgt tcccgatctg cacgataaat acggccgcgc ctttgaacag gcctatatcg
     2521 gctacgaaga aaaggcggcg cggggtgaac ttgccctgtt taaaacaatt ccggctctat
     2581 cgctatggcg caagatgcta tccatgctgt tcgaaaccgg ccatccgtgg atcaccttta
     2641 aagatccttg caacatccgc tcgccccaac agcacgtcgg cgtcgtgcac agttcgaacc
     2701 tgtgcactga aatcacgttg aacaccagcg attcggaaat cgcggtgtgc aatctgggtt
     2761 ccgtaaactt ggccgctcat ctcaagaaag acgccaacgg ccaatacgtg ctcgatcaca
     2821 gcaagctgga acgcacaatc accaccgcca tgcgcatgct cgataacgtc atcgacatca
     2881 actattacgc agtcgccaag gcgcgcaact ccaatgcccg ccatcgcccg gtcggattgg
     2941 gcatcatggg atttcaggat tgcctgcata ggatgcgtat tccgttcgct tcacaggcgg
     3001 ccgtcgaatt ttccgaccga tccacagaag cagcctgcta ttacgcctac tgggcctcca
     3061 gcaaattggc ggccgaacgc ggcgcctacg ccagcttcaa aggctcgctg tgggatcgcg
     3121 gcattttgcc gcaagacacg ctggctctac tgcgcgacga acgcggcg
//
LOCUS       DATCXU010000060         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_851080,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000060 DATCXU010000000
VERSION     DATCXU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851080"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VIR04_01285"
     CDS             complement(<1..298)
                     /locus_tag="VIR04_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY9459412.1"
                     /translation="MHKQMRSALFVPATRPERFLKALGTGADMVIIDLEDAVERDNKD
                     QARRHLTEFAQSNPDVSFLVRVNGATTSWFESDLAACGHFANIVGILLPKAESAG"
     gene            complement(298..1497)
                     /locus_tag="VIR04_01290"
     CDS             complement(298..1497)
                     /locus_tag="VIR04_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY9459413.1"
                     /translation="MASAATHPRPLDGVTVISLEHAIAAPFCTRQLADMGARVIKIER
                     PGVGDFARGYDDRVRGLASHFVWTNRSKESLTLDLKKDEAKTILFRLLDQADVLVQNL
                     APGASARMGLSFDALHESHPRLIVCDISGYGEGGPYQDKKAYDLLIQSEGGFLSVTGS
                     PDGPAKAGCSISDIAAGMYAYSSILSALLLRERTGQGSRLDVSMLESMVEWMGFPMYY
                     AFDGAPPPPRAGASHATICPYGPFPVGDGSEIMLGLQNEREWAAFCKIVLKQPELAKD
                     ARFTSNLARVENKEALCALIVKAFATLTREQALQRLEDAQIANASVNTMRDVWAHPQL
                     AARKRWREVSSQSGPLPALIPPASSSAFEARMDAVPSLGEHRASILAELGYSADDVQA
                     LLRQKVI"
     gene            complement(1533..2363)
                     /locus_tag="VIR04_01295"
     CDS             complement(1533..2363)
                     /locus_tag="VIR04_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="itaconyl-CoA hydratase"
                     /protein_id="HEY9459414.1"
                     /translation="MTLPDPASWVGHTETCADSIDAGHVEKIALSLAAPVPASGSALP
                     LLWHWALFVKVLSYDQLGDDGHPRRGGFLPPAEHRNRMWAGGRLTFIEPLRISVPANR
                     VSTVAAVNEKQGRTGKLLFVTVKHEYFQSDRLCISEEQDIVYREPSPPKLAGTQPAPS
                     GQWSETIEPSAIMLFRYSAVTFNGHRIHYDAPYATGVEGYPGLVVHGPMIATLMCGAF
                     QRANPDKVVTSLSYRGLRPLTAPQAFHVAGALGEPGCARLWAERDGTLAHQAELRFKV
                     "
     gene            complement(2465..3991)
                     /locus_tag="VIR04_01300"
     CDS             complement(2465..3991)
                     /locus_tag="VIR04_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY9459415.1"
                     /translation="MATVIQEEDLVQSIADAVQFISFYHPADYLRHLARAYEREESVA
                     AKDAIAQILTNSRMCAEGHRPICQDTGIVNIFLKVGMGVRFESRLGLQELCDQGVRRG
                     YLNLDNPLRASIVGDPLFNRKNTGDNTPCMVSVELVAGDRVDVQIAAKGGGSENKSQF
                     VMLNPNDSLVDWVLKTLPKMGAGWCPPGMLGIGVGGTAEKAMLMAKQSLMEDINMYEL
                     LQRGPENKLEELRIELYEKVNALGIGAQGLGGLTTVLDVKINTFATHAASKPVAMIPN
                     CAATRHIHFALDGSGVAEFAPPPLSDWPDVHWTPNYKQSLQVDLNALTQDQVASWKPG
                     QTLLLSGKMLTGRDAAHKRIQTMLERGEALPVDFRNRVIYYVGPVDPVRDEVVGPAGP
                     TTSTRMDEFTEMMLSQTGLISMIGKAERGPVAIEAIRKHGAAYLMAVGGAAYLVSKAI
                     RSARVVGFQDLGMEAIYEFDVKDMPVTVAVDSTGSSVHETGPEVWRAKLAKKPTVRFQ
                     "
     gene            complement(3994..4761)
                     /locus_tag="VIR04_01305"
     CDS             complement(3994..4761)
                     /locus_tag="VIR04_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY9459416.1"
                     /translation="MSDLIKIEVIDQIQIMTINRPQTRNAINYETAQEFARALDRLDE
                     SDEIVLGIVTGAGGTFSSGMDLKAFARDGQRPYAGDRGFAGLCEKPPAKPLIAAVEGY
                     ALAGGCEVALACDLIVAANNASFGLPEVKRGIVPGAGGMLRLPRRIPYHVAMEMILTG
                     EMMPARRAYDIGLVNRLFEPGKALEGALTLARIIAQNGPLAVRTAKKVVSRSSDWRQA
                     EMFDLQRPRIASIFSSSDAREGATAFAEKRKPVWQGK"
     gene            4930..5838
                     /gene="glyQ"
                     /locus_tag="VIR04_01310"
     CDS             4930..5838
                     /gene="glyQ"
                     /locus_tag="VIR04_01310"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY9459417.1"
                     /translation="MLTFQQIILTLQHYWDQQGCALLQPYDMEVGAGTSHTATFLRSI
                     GPEPWRAAYVQPSRRPNDGRYGENPNRLQHYYQFQVVLKPAPPNILDLYIQSLEALGI
                     DPKIHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCALTTGEITY
                     GLERLAMYLQGVESVYDLVWTEGLNGQRILYGDVFHQNEVEQSTYNFEHACVPMLFGH
                     FNDYENEAKRLIDVPLALPAYEATLKAAHTFNLLDARGAISVTERAAYIGRIRNLSRA
                     VAQAYFDSREKLGFPMLDKRLDAKAA"
     gene            5838..>6270
                     /locus_tag="VIR04_01315"
     CDS             5838..>6270
                     /locus_tag="VIR04_01315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY9459418.1"
                     /translation="MASATAQNRPLLLELLTEELPPKALHELGIAFAEGLRSTLDKQH
                     LLAPGCVVVDFATPRRLGVHLSAVREQAPEHNYTEKLMPAKVGLSADGGATPALLKKM
                     ASKGLSHLTTADLIHESDGKQDYLYAKGTNAGATLQAGLQAA"
BASE COUNT         1365 a         1927 c         1680 g         1298 t
ORIGIN      
        1 gcccagccga ttcggccttg ggcaacagaa tgcccacgat attggcaaag tgcccgcatg
       61 cggcaagatc cgattcaaac catgatgtcg tcgcgccgtt gacgcgaacc aaaaacgaca
      121 cgtccgggtt gctctgcgca aactcggtca gatgccggcg cgcctgatct ttgttgtcac
      181 gctccacggc atcttccaga tcgataatca ccatgtcagc gccggtgccc agcgctttta
      241 aaaaccgctc gggccgcgtg gccgggacaa acaaggcaga acgcatttgc ttgtgcatca
      301 aatcaccttt tgccgcagca gcgcctgcac atcgtcagcg gaatagccca actcggcgag
      361 gatactggcg cgatgctcgc ccagcgacgg gaccgcgtcc atgcgagcct cgaaagcaga
      421 actgctggcg ggcggaatca aggcgggcag cggacccgat tggctgctca cctcgcgcca
      481 gcgcttgcgc gcggcaagct gaggatgcgc ccagacatcg cgcatcgtgt tgacgctggc
      541 gttggcgatt tgagcatctt ccagccgttg cagcgcctgt tcccgagtaa gcgttgcaaa
      601 cgccttcacg atcagcgcac atagcgcttc tttattttcg acgcgcgcaa ggttagacgt
      661 aaagcgtgca tctttcgcca gttccggctg ctttagaact attttgcaaa aagccgccca
      721 ttcgcgctcg ttttgcaagc ccagcataat ttcgctgcca tcgcccaccg gaaagggacc
      781 gtaggggcag atcgtggcgt gcgaggcgcc ggcgcgcggt ggcggaggcg ctccatcgaa
      841 tgcgtaatac atggggaacc ccatccattc aaccatgctc tcaagcatgg atacatccag
      901 acggcttccc tggccggtgc gctcacgcaa caacaacgcc gacaggatgc tgctgtatgc
      961 gtacatgccc gcggcaatgt ccgagattga acatccggct ttggccgggc catcgggcga
     1021 tcccgtgacc gataaaaacc cgccctcgct ttgaatcagc aagtcgtacg cctttttgtc
     1081 ctggtaaggg cctccctcgc cgtaacccga aatatcgcag acgatcaaac gcggatgcga
     1141 ctcgtgcaag gcgtcaaacg acaaacccat gcgagccgac gcccccgggg caaggttttg
     1201 caccagcaca tcggcctggt caagcaggcg gaataaaatc gtcttcgcct cgtctttctt
     1261 caagtcgagc gtcaagcttt ccttcgagcg attcgtccag acaaaatgcg aggccaggcc
     1321 ccgcacccgg tcgtcgtaac cacgggcgaa atcgcccacg ccgggccgtt caattttaat
     1381 gacgcgggcg cccatgtcgg ccagttggcg agtgcaaaat ggagcggcaa tggcatgttc
     1441 caggcttatc accgtgacac cgtccagcgg tctggggtgg gtcgccgcac ttgccatcgt
     1501 cgattccgtg cgcgcattta tcttggtgtg agtcatacct taaaccttag ttcagcctga
     1561 tgcgccaggg tgccatcgcg ctccgcccaa agacgggcgc aaccaggctc gcccaacgca
     1621 cctgcgacat ggaaagcctg cggagccgtc aggggccgca agccacgata agacagcgat
     1681 gtgacaactt tgtcgggatt ggcccgctga aacgcgccgc acatcagggt ggcgatcatc
     1741 gggccgtgca cgaccagccc cggatagccc tcgacacccg tggcatacgg cgcgtcgtag
     1801 tgaattcggt gaccattgaa cgtgacggcg gaataacgaa acagcataat cgcgctgggc
     1861 tcgatcgttt cagaccactg cccgctgggg gcgggttgcg tgcccgccaa tttcggtggg
     1921 ctgggttcgc gataaacaat gtcctgctct tcggaaatgc acaagcgatc actctgaaaa
     1981 tactcgtgtt taactgtcac gaacaatagc ttgccagtgc gtccctgctt ctcgttgaca
     2041 gccgcgaccg tcgaaacgcg attagccgga acgctgatcc gcaacggttc gataaacgta
     2101 agccgtccgc ccgcccacat tcggttgcgg tgctcggcgg gtggcaaaaa gccgccgcgc
     2161 ctgggatggc catcgtcgcc aagctgatca taagacaaca ccttcacgaa cagggcccaa
     2221 tgccacagca atggcagcgc gctgccggac gccggaaccg gcgcggcaag acttaacgct
     2281 attttttcga catgccccgc atcgatagaa tcggcgcaag tctcggtatg accaacccat
     2341 gaggcaggat ccggcaaagt catgacttcc ttcttacagt cgcgttaggt gtttatagta
     2401 atgccagcca tcagtttgcc tgcgggatcg cacttttaga attcgatttt ggaaaagagc
     2461 gcctttactg aaaacgaacc gtcggcttct ttgccagctt ggcgcgccaa acctcgggcc
     2521 ccgtttcatg cacggaactg cccgtggaat ccactgccac ggtcaccggc atgtctttta
     2581 catcgaactc atagatcgct tccatgccga ggtcctggaa cccaacaacc ctggcgctgc
     2641 gaatggcctt ggataccaga taggccgctc cgcctaccgc cattaaatac gccgcgccgt
     2701 gcttgcgtat ggcttcgatc gcaacgggac cgcgctcagc tttgcctatc attgatatca
     2761 agccggtttg actcaacatc atctcggtga attcgtccat gcgcgtcgac gtggtcgggc
     2821 cggccgggcc aacgacctcg tcgcggaccg ggtcaaccgg gccaacgtaa taaataaccc
     2881 gatttctgaa gtcaaccggg agcgcttccc cgcgttcgag catagtctga atacgcttat
     2941 gggcggcgtc gcggccggtc agcatttttc ccgacaacag caaagtctgg ccaggcttcc
     3001 aactggccac ctggtcttgg gtcagcgcgt tcaaatcgac ttgcaacgac tgtttgtaat
     3061 tgggagtcca gtgaacatcg ggccagtccg acagaggcgg cggcgcaaat tcagcaacgc
     3121 ccgagccatc aagcgcgaag tgaatgtgcc gggtggcggc gcaattggga atcatggcaa
     3181 ccggcttgga ggccgcatgc gtggcaaagg tgtttatctt tacatccaga accgtcgtca
     3241 agccgcccag gccctgggcg ccaattccca gcgcattcac tttctcgtag agttctatcc
     3301 ttaattcttc aagcttgttt tcagggccgc gctgcaatag ctcatacatg ttgatgtctt
     3361 ccatcaggga ttgcttggcc atcagcatag ccttttccgc ggtccctccg actccaatgc
     3421 ccagcatgcc tggcgggcac cagcccgcgc ccattttcgg gagtgtcttc aacacccagt
     3481 caaccagcga atcgttcgga ttaagcatga cgaattgcga tttattttca gatccgcctc
     3541 ctttcgcggc tatctgcaca tcgaccctgt cgcccgcaac cagttcaacg ctgaccatgc
     3601 acggcgtgtt atccccagtg ttcttgcgat tgaacaaagg gtcgcccacg atcgatgcgc
     3661 gcaacgggtt gtcaagattc aaatagcccc ggcgcacgcc ctggtcgcat aattcctgca
     3721 agccaagtcg cgactcgaac cgcacgccca tgccgacttt caaaaagatg ttcacaatgc
     3781 cagtgtcttg gcaaattggg cgatgtcctt cggcgcacat gcgtgaattg gtcagaatct
     3841 gtgcgatcgc gtctttggcg gccacgcttt cttcgcgctc gtaggcgcgc gccaaatggc
     3901 gcaggtagtc cgccggatga taaaaactga tgaactgcac cgcgtctgct atggactgga
     3961 ccaggtcttc ttcctgaatg acggtcgcca tatttattta ccctgccaga ccggtttgcg
     4021 tttttcggca aacgccgtcg ccccttccct ggcgtcggaa gacgaaaaaa tggaagcaat
     4081 gcgcgggcgc tgcaaatcga acatttcggc ctggcgccag tcgctggacc ggctcacaac
     4141 cttttttgcg gtccgcaccg cgagcggtcc attctgagca atgatccgtg ccaacgtcag
     4201 tgccccctcc agcgccttgc cgggttcgaa caagcgattc accaacccaa tgtcatacgc
     4261 gcgccgggcc ggcatcattt cgccggtaag aatcatttcc atggcaacgt gataaggaat
     4321 acgccggggc aaacgcaaca tgccgcccgc gcccggaacg atgccgcgct tgacctcggg
     4381 caagccaaag ctggcattgt tggcggccac gatcagatcg catgccaacg ccacttcaca
     4441 gcctccggcc agcgcgtaac cctcgacagc ggcaataagt ggcttggccg gcggcttttc
     4501 acacagcccc gcaaagccgc gatcccccgc ataagggcgc tgaccgtccc gcgcaaacgc
     4561 ttttaaatcc atgcccgagg aaaacgtgcc gcccgcgccc gtcacaatgc ccagaacgat
     4621 ttcatcgctt tcgtccagcc gatcaagcgc acgcgcgaac tcttgcgctg tctcgtaatt
     4681 aatcgcattg cgcgtttgcg gacgattgat cgtcatgatc tgaatctgat caataacttc
     4741 tatcttaatc aagtcggaca tagaactatt cctgaaaaaa cggcttgagg ccagtcatcg
     4801 aaacactgcg gtgaacgccc tatcatacgg ccaattcagg cctgcttcag ccagtgctta
     4861 tcctgcttta ttttggaagc agttaaaatt aaacacttta tcgacttttc gcttcactgg
     4921 ctttcgttca tgctcacctt tcagcaaatt attctgacgc ttcaacatta ctgggatcaa
     4981 caaggctgcg cgcttttaca accgtacgac atggaggtcg gtgcaggcac ctcgcacact
     5041 gctacgtttt tgcgatcaat cggacccgag ccatggcgag ccgcgtacgt ccaaccatcg
     5101 cggcgcccca acgacggccg gtatggcgaa aaccccaatc ggttgcaaca ttattatcaa
     5161 ttccaggtcg tgctcaagcc agcgccgccc aacatccttg atctgtatat ccagtctctc
     5221 gaagctctgg gcatcgatcc taaaattcac gatatccgat tcgtcgaaga cgactgggaa
     5281 aaccctacac tcggagcatg gggcctgggt tgggaagtct ggttaaacgg catggaagtc
     5341 acccaattca cgtacttcca gcaagttggc ggtctggact gcgccctgac gactggcgag
     5401 attacttacg gtctggaaag gctggcgatg tatttgcaag gcgtcgaaag cgtttatgat
     5461 ctggtctgga ccgaagggct caacggacag cgcatcttgt atggcgatgt gttccaccaa
     5521 aacgaagtcg agcaatccac ctataacttc gagcatgcct gcgtccccat gctgttcggc
     5581 catttcaacg attacgaaaa cgaagccaaa agattgatcg acgtcccgct tgcattaccc
     5641 gcgtacgagg ctacgcttaa agccgctcat acctttaatt tgctggatgc gcgcggcgcc
     5701 atcagcgtca ccgagcgggc ggcctatatc ggccgcattc gcaacttgtc gcgcgccgtg
     5761 gcacaggctt acttcgactc gcgtgaaaaa ctcgggtttc cgatgctcga caagcgcctt
     5821 gacgcgaagg cggcgtgatg gcgagcgcga ccgcacaaaa ccgtcctttg ctgcttgaac
     5881 tgcttaccga agaattgccg cccaaagcac tgcacgaact tggcattgcc ttcgccgaag
     5941 gcctgcgttc cacactggac aagcagcatc tgcttgcgcc gggctgcgtc gtcgttgatt
     6001 tcgccacgcc gcgccggctc ggcgtgcacc tgagcgccgt gcgcgagcaa gctcccgaac
     6061 ataactacac cgaaaaactt atgcccgcca aggtcggctt gtccgctgat ggcggcgcaa
     6121 cgcccgccct actgaaaaag atggcgagca agggcctgag ccatctgaca accgccgatc
     6181 tgattcacga gtccgatggc aaacaagatt atctgtacgc caaaggaaca aatgcggggg
     6241 ccactcttca ggcgggactg caagcggcgc
//
LOCUS       DATCXU010000061         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_624793,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000061 DATCXU010000000
VERSION     DATCXU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624793"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VIR04_01320"
     CDS             complement(<1..761)
                     /locus_tag="VIR04_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEY9459419.1"
                     /translation="MDKFLIAALYKFVHLADYQALQEPLLACCLANQVTGTLLLAEEG
                     INGTIAGSVSGVEAVLDHMRQDSRLQSLPYKKSWADQSPFYRMKVKLKREIVTMGVPN
                     IHARAEAGTYVKPDQWNQLIQDPDVVVVDVRNDYEVSIGSFESAVNPHTASFSEFPDW
                     VQRQSGQGGILNKKTKVAMFCTGGIRCEKSTAYLRSKGFDEVYHLEGGILKYLETVSA
                     GESRWNGECFVFDERVSVVHGLQKGSYDLCRACRLP"
     gene            complement(782..940)
                     /locus_tag="VIR04_01325"
     CDS             complement(782..940)
                     /locus_tag="VIR04_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459420.1"
                     /translation="MLVKPRTPTKFRPLQAGVQQVINEERAKKASAKRIISMGGVGNE
                     RYGRKRDK"
     gene            complement(1030..1938)
                     /locus_tag="VIR04_01330"
     CDS             complement(1030..1938)
                     /locus_tag="VIR04_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9459421.1"
                     /translation="MDFRQLNYFVAVAQALSFSRAARNLHISQPPLSQQIKLLERDLG
                     VQLFDRSRRAVELTHAGTLFYAQAVGILERYASAKELCAWSADGRAGKLRIAFTASVP
                     IFEAFPRLIHSFHAAYPRIEMDLLHMSTGEQLVALDDQQIDVGFLRPSLNFRAASTMD
                     VLDLWRDELVLATAKCDEGDLQDGSVNLGDYARRDFILFPQALGCGLFAHIVALTTES
                     GFSPRIVQEARENSTTLALVAAGLGISIVPDTYACVAPPGVQFRRIESPNAKSKIVLA
                     SARNHETASLGLFLEHVRVFLKRCTL"
     gene            2052..3035
                     /locus_tag="VIR04_01335"
     CDS             2052..3035
                     /locus_tag="VIR04_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459422.1"
                     /translation="MKLTALRTFLALTAGTLVGFAPFQSALCADYPEKAVNIVVPYSA
                     GGGVDIVTRLVVKKMSDELKRPFVVENRPGAGTNIGMAYVARAKPDGYTLYTASNTFT
                     TNKALYSSLSFDPATAFTSVGRIGEAPLVIVVPEQSPYKMLSELVEYGQQHPGDLTFG
                     TAGMGSSGHTASELFTRAGKFKALHVPYKGGSPAITDLLGGRLSFMVINPLEVISHIK
                     AGKLRALAVLNKKGTPLLPELKTAEKLGVPNLEATVWWGIVAPAGTPEPIITKLNAAL
                     NTALRDPDVIKTLHTLGAEPLPGSASDFHSFLVKNTAELTEVIKAANIKVD"
     gene            3066..3782
                     /locus_tag="VIR04_01340"
     CDS             3066..3782
                     /locus_tag="VIR04_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY9459423.1"
                     /translation="MSQKNALIISAHSADFVWRAGGAIALYAERGWNVHVLCLSFGER
                     GESAKLWKQQGMTLDAVKRARQEESQKAAKILGATVSFFDCGDYPLRVDDQTLLRIAD
                     LYRQLRPEFVLTHSFKDPYNFDHPLANRLALEARIIAQAHGHNPSEPVIGAPPVFLFE
                     PHQSEQCEWKPDLFLDISSVWETKYRAFQAMGAQEHLWEYYDRLGLQRGAQAVRNSGK
                     KIKYAEAFQSVYPRVVDDLA"
     gene            3842..4546
                     /locus_tag="VIR04_01345"
     CDS             3842..4546
                     /locus_tag="VIR04_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY9459424.1"
                     /translation="MMINSAFERVSKGQVARAKEIASTILADVAGRRGALDGRIKPVS
                     PTMRVCGPALTVEVRPGDNLMIHAALVLARPGDVIVVDGKADTTCALMGELMCTHARA
                     AGVAGVVIDAAVRDLAELKTAPFPVFACGSNPNGPTKRIGGRIGHAISVGGVAVEPGD
                     LVVGDEDGVVIIPKADVEQVLTDGLKKIAAEHKRLEDIAAGRLIYGWLEGTLKEVGAL
                     PAGKSLPQLIADFAAK"
     gene            complement(4557..>5059)
                     /locus_tag="VIR04_01350"
     CDS             complement(4557..>5059)
                     /locus_tag="VIR04_01350"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEY9459425.1"
                     /translation="SQTTAAAAGALGVVRRDPFAMLPFCGYNMGDYFSHWLKLGDRLR
                     AAGAVLPRIFCVNWFQTDDQGKFIWPGYGENMRVLKWMLERIQGTAQGEDSLFGVSPR
                     YQDITWDGLDFSADKFKRITTIDPVAWRKELDLRDALFDKLKKRLPAVLSDLQDQLRE
                     RLKARV"
BASE COUNT         1184 a         1462 c         1340 g         1073 t
ORIGIN      
        1 ggcagacggc aggcgcggca caggtcgtag ctgccttttt gcagcccatg cacgaccgat
       61 acccgttcat caaatacgaa acactcgccg ttccagcggc tttcgcccgc ggaaaccgtt
      121 tccaaatact tgagaatgcc accctcaagg tgatagacct cgtcaaaacc ttttgatcgc
      181 aaataagcgg ttgatttctc gcaacgaata ccgcctgtgc aaaacatggc cactttcgtt
      241 ttcttgttca gaattccgcc ttggcccgat tggcgttgca cccagtcggg gaattcggaa
      301 aagctggcgg tatgcggatt tacggctgac tcgaacgaac cgatcgatac ctcgtaatcg
      361 ttgcgtacat caaccaccac cacgtcgggg tcctgaatca attggttcca ttggtcgggt
      421 ttgacgtaag tgccggcttc ggcccgagca tgaatgttgg gcacccccat cgtgacgatc
      481 tcgcgtttca gcttgacctt catccgataa aacggcgatt gatccgccca tgacttctta
      541 tagggcaacg actgcagacg cgaatcctgc cgcatgtggt caaggaccgc ttcaacacct
      601 gaaactgatc ccgctattgt tccgttgatg ccctcttcag ccagcagcag cgttccagtg
      661 acctgatttg ccaggcaaca cgccagcaga ggctcctgca acgcttgata atcggcaaga
      721 tgaacgaact tgtacaacgc cgcaattaaa aatttatcca tgctgtcgag ggcaaacggc
      781 tttatttatc ccgcttcctt ccgtaacgtt cattgcccac gccccccatg ctgatgatgc
      841 gcttggcaga cgcctttttg gctcgttctt cgttgatgac ctgctgcacg cccgcctgaa
      901 gggggcgaaa cttggttggg gtacgcggtt ttacaagcat atataagcct cacgattaaa
      961 gaaaaaccct ttgaaggcgc tctagtcatg acgtcggccg ttgtttcttt ttgtgacgtc
     1021 tcatccaccc tatagggtac accgtttcaa aaaaacccgc acatgttcca aaaaaagccc
     1081 caacgacgcg gtttcatggt ttctggcact ggccagtacg attttcgatt tcgcatttgg
     1141 gctttcaatg cgtctgaatt gcacacctgg cggcgcgacg cacgcgtagg tatccggaac
     1201 tatggaaatt cccaagcctg ccgccaccag cgccagtgtt gtgctgtttt cgcgagcctc
     1261 ctgaacaatg cggggcgaaa aacccgattc agtcgtaaga gccacaatat gcgcgaacag
     1321 gccgcagccg agagcctgcg gaaacaaaat aaaatcgcgg cgagcatagt cgcctaaatt
     1381 gacagatccg tcttgcagat ccccctcgtc gcacttggcc gtggccagca ccagctcgtc
     1441 gcgccacaaa tccagtacat ccatcgtcga agcagcgcgg aaattcagag acggacgcaa
     1501 aaagccgacg tcaatctgct gatcgtccag agcaaccaac tgctcgccgg tcgacatgtg
     1561 caacaaatcc atttcaatcc gtggataagc cgcgtgaaag ctgtgaatca gtctggggaa
     1621 agcctcaaaa atcggtacag atgccgtaaa cgcaatgcgc aacttgccag ctctgccgtc
     1681 ggcggaccag gcgcacagtt cctttgcgct ggcatatcgc tcaagaattc ccaccgcctg
     1741 cgcgtaaaac aaagtgcccg cgtgcgtcaa ctcaactgcg cggcggctgc gatcaaaaag
     1801 ttgcacgccc agatcgcgct ccagcagttt tatctgttgg ctgagcggcg gctggctgat
     1861 gtgcaaattt cgcgcggcgc gactaaaact caatgcctgg gcgacggcca cgaagtaatt
     1921 taattgtctg aaatccatac ttattcgatt agcgtatagg ttgcaagttt aaatagtatt
     1981 tgaccctata agtgcttcgc tacaaaatct gccttgaatc catataaccg cgacccactt
     2041 ttgaggcgac catgaaactg acggcattac gaaccttcct tgcgctgacc gcgggaaccc
     2101 ttgtcgggtt tgcgcccttt caatcggccc tttgcgccga ctacccggaa aaagcagtca
     2161 acattgtcgt gccctattcc gcgggcggag gcgtggacat cgtaacgcgc ctggtcgtca
     2221 agaagatgtc cgacgaactg aaacgcccct tcgtggtgga aaaccgtccc ggagccggca
     2281 ccaatattgg catggcctat gtggcacgcg ccaaaccaga tggttacacg ctgtataccg
     2341 cctccaacac attcaccaca aacaaggctc tatattccag cttgagtttc gatcccgcca
     2401 ctgcctttac gagcgtcgga cgtattggag aagccccttt ggtgatcgta gtgcccgaac
     2461 agtcgcctta taaaatgcta agcgagcttg tcgaatacgg gcagcagcat cccggcgacc
     2521 ttactttcgg cacggcgggg atgggtagct ccggccacac cgccagcgaa ctattcaccc
     2581 gagcggggaa attcaaagct ttgcacgttc cctacaaagg cggctcgccg gccattaccg
     2641 atcttctggg cgggcgcttg tcattcatgg tcatcaatcc gctggaagtc atttcccaca
     2701 tcaaggccgg caaattgcga gcgctggctg ttctgaacaa gaaaggcacg cctttgctcc
     2761 ccgaactgaa aacggcggaa aagctggggg tccccaatct tgaagccacc gtctggtggg
     2821 gaattgttgc gccggccggt acgccggagc caataataac gaagcttaac gcggcgctca
     2881 ataccgcgct gcgcgacccc gatgtcataa agaccttgca cactttaggc gccgaaccac
     2941 tgccgggaag cgcctcggac tttcattcct ttctcgttaa aaatacggcc gaactgaccg
     3001 aagtcatcaa agcggccaat attaaggtcg actaacactg tcaacacagc attgggaata
     3061 cgtcgatgag tcagaaaaat gcccttatta tcagcgcgca ctccgcggat tttgtctggc
     3121 gcgcgggcgg cgccatcgct ctatatgccg agcgcggctg gaatgtgcat gttctttgcc
     3181 tgtccttcgg tgaacgcggc gaatcggcga aactgtggaa gcaacagggc atgactctgg
     3241 atgcggtgaa acgcgcaaga caggaggaat cgcaaaaggc cgccaaaata ctgggcgcca
     3301 cggtttcgtt tttcgattgt ggcgactatc cattgcgggt cgacgatcag acgcttttaa
     3361 gaattgccga cctgtatcgc cagctcaggc ctgagttcgt gctgacgcac tctttcaaag
     3421 atccctacaa tttcgatcac cctctggcaa atcgcctggc acttgaagcg cggatcatcg
     3481 ctcaggccca tggtcacaac ccttcggagc ccgtaatcgg ggcgccgccg gtgtttctct
     3541 tcgagccgca ccaatcagag cagtgcgaat ggaaaccgga cctgttcctg gatatttcgt
     3601 ccgtctggga aaccaaatac cgcgccttcc aggcaatggg cgctcaggaa cacttgtggg
     3661 agtactacga tcggcttggg ctgcaacgtg gcgcgcaagc cgttaggaat tccggtaaaa
     3721 agatcaaata cgcagaagcg ttccaaagcg tgtatccgcg cgttgtcgac gatctggcct
     3781 aatccgcccg aataacggcc aaccggaagc aatggcgcac tgagttaaaa aggactgaag
     3841 catgatgata aacagcgcat tcgaacgagt atcgaaaggc caggtcgcgc gagccaaaga
     3901 aattgcctcc accatactgg cggacgtggc tggacggcgc ggagcgcttg acggtcgaat
     3961 caaacctgtg tcaccgacaa tgcgtgtatg cggcccggct ttaacggtcg aggtgcggcc
     4021 tggcgacaat ttaatgattc atgccgctct tgtgctggcg cggcctggag acgtcatcgt
     4081 cgttgacggc aaggccgata ccacctgcgc gctgatgggt gaactgatgt gtacgcatgc
     4141 ccgggcggcg ggtgtggcgg gtgtggttat cgacgccgca gtgcgcgact tggctgagct
     4201 caaaaccgcg ccattccccg tttttgcttg cgggtcgaac cccaatggcc ccaccaaacg
     4261 gatcggcggc cgtattggcc atgcaatttc agtcggaggc gtcgccgtag aacctggcga
     4321 cctggtggtc ggcgacgaag atggcgtcgt catcatccca aaagccgatg tcgaacaggt
     4381 gctgaccgac ggattgaaaa aaatcgccgc cgagcacaaa cgcttggaag acattgccgc
     4441 aggacgatta atttatggct ggctggaagg cacgctaaaa gaagttggcg ctttgccggc
     4501 cggaaaaagc ttgccgcaac taatcgccga cttcgcggcg aaataaaatc cggccttcaa
     4561 acccgggcct tcagacgttc gcgcaactgg tcttgcagat ccgacaaaac cgcaggcagg
     4621 cgctttttta acttgtcgaa cagggcgtcg cgcaagtcca gctccttgcg ccatgcgacc
     4681 gggtctattg tcgtaatgcg cttgaacttg tccgcgctga aatccagccc gtcccatgtt
     4741 atatcctggt agcggggcga gacgccaaac aagctgtctt cgccttgggc ggtgccctga
     4801 atgcgctcga gcatccattt cagcacacgc atattctcgc catatccggg ccagatgaac
     4861 ttgccctggt catcggtctg aaaccagtta acgcaaaaaa tccgcggcaa gacagcgcct
     4921 gccgcccgca atcgatcgcc cagtttcagc caatgcgaaa agtaatcgcc catgttgtag
     4981 ccgcaaaaag gcaacatggc gaaggggtcg cgacgcacca cgcccagcgc acccgcagcg
     5041 gcagccgtcg tttgagaac
//
LOCUS       DATCXU010000062         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1162530,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000062 DATCXU010000000
VERSION     DATCXU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162530"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VIR04_01355"
     CDS             complement(<1..1841)
                     /locus_tag="VIR04_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY9459426.1"
                     /translation="MRLTQIKLAGFKSFVELTVIPTPSQLVGVVGPNGCGKSNIIDAV
                     RWVLGETKASELRGESMQDVIFNGSGNRKPAARASVELVFDNSQGRAAGQWSVYAEIA
                     VRRVLTRDGASSYFVNGQQVRRRDIHDIFLGTGLGTRGYAIIGQGMINRLIEARPEEL
                     RIYLEEAAGVSRYKERRRETESRLSDTRENLTRVEDILRELAGQLEKLDAQAAVARQY
                     RALQDEGGQKQSALWFLKESGAREDQRARLAALEQAQTQLEGAIAGLRASEAAVESRR
                     QAHYAAGDAVHTAQGLLFEAGAQVSRLEAEIHHVVDSRNRLQDRRTHLQQQIQEWTDQ
                     QTHCTEEMARTRDEFEIRQARTQQARERVDEAQAGLPDIETQVRNAADARDEMRVALA
                     RVEQNLALAAQAQRDSDRQLAALELRRERLSQELHNLDMPDPAHLERLAGSHVTLDGQ
                     VRQAQDELEQLEARLPALDHERGRVQADSHRQAEELARSEARLAALNKFQDDIQKRGA
                     LQPWLDKHELSGLARLWQKLQIESGWETALETVLRERMAALEVRQLEHARAFIDDAPP
                     ARLAFYHVPMASGALALPEPGLTPLSSLLRLADPDLRSLIGGWVRDV"
     gene            2104..3408
                     /gene="lplT"
                     /locus_tag="VIR04_01360"
     CDS             2104..3408
                     /gene="lplT"
                     /locus_tag="VIR04_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY9459427.1"
                     /translation="MNKGFFLVMTAQALSSLADNALLIAAIALIAELQGPDWMAPMMK
                     WWFALAYVALAAFVGAFADSFPKGRVMFITNAIKLLGCLMMFSYASFGLTHSEQLLLV
                     FCAYTLVGIGAAAYSPAKYGIVTEMLPPDLLVKGNSWIEGLTVLSIILGTVLGGVLIS
                     PSVSLMLLSHPWISHFVHNGVEAAIFLIGFVYFLAAVCNLLIPLTGVSYPKQQTNPVQ
                     LVRTFKSYVRILWHDKLGQISLAVTTLFWGAGATLQFIVIEWGARHLGYRLDQASILM
                     GVAAVGTVIGSVAAGRVPLKRALSVLPVGVLMGVAVLLMPLVTETWAVYSLLLIIGAL
                     SGFFVVPMNALLQHRGHVLLSAGHSIAVQNFNEQLNILLMLAVYSAMLWLNLPINFII
                     AIFGVLVSALMLVFMHWNRLNHGKDPGLENQIGQHGHGQALH"
     gene            complement(3447..4226)
                     /locus_tag="VIR04_01365"
     CDS             complement(3447..4226)
                     /locus_tag="VIR04_01365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY9459428.1"
                     /translation="MVDLDEPAALRLSAVQRAWLREIGIDKNLLGRFDASGADTVTTV
                     PERVAIDQPVGTKALLRAASPVSDSLPHDLESLRSLVAICEACPLYAGRSRTVFGSGA
                     TESLQWMIIGEAPGDSDDRAGLPFQGAAGTLLHAMLAAVGVRADSSVFFTNLVKCRPL
                     GNRPPGPAEIAACLPYLRRQIALLNPRRILTLGRLAAQTLLNDDSDFEQLRGCVHELL
                     SESGAKIPLVATYHPAYLLSRPQYKAGAWSDLILAYTALCD"
BASE COUNT          896 a         1377 c         1145 g          939 t
ORIGIN      
        1 taaacatcac gcacccaccc gccaatcagc gagcgcaaat cgggatcagc aaggcgcaac
       61 aacgaactta aaggagtcag gcccggctcc ggcagcgcca gcgcgcccga agccattggc
      121 acatgataaa acgccagacg cgccggtggc gcgtcatcga taaacgcccg ggcgtgctca
      181 agctggcgca cctcaagcgc ggccatgcgt tcacgcaaca ccgtttcaag cgccgtctcc
      241 caacccgatt cgatttgcag tttttgccac aaacgcgcca ggccggacaa ttcatgttta
      301 tcgagccaag gctgcaatgc gccccgcttt tgaatatcgt cctgaaattt gtttaatgca
      361 gccagacgcg cttccgatcg ggccagttct tccgcctgcc gatgggaatc ggcctgcacg
      421 cgaccgcgct catggtcgag ggctggcagg cgggcctcaa gctgctccaa ttcgtcctgc
      481 gcctgccgga cctgcccatc aagcgtcacg tgactgccgg ccagacgctc caggtgcgcc
      541 gggtcgggca tatccagatt atgcaattcc tggctcaaac gttcccggcg caattccagc
      601 gccgcaagct ggcggtcaga gtcgcgctgc gcttgcgccg ccaacgctaa attctgctcg
      661 actcgcgcca gcgcaacacg catctcatcg cgtgcgtccg ccgcatttcg gacctgcgtc
      721 tcgatatcgg gcagccccgc ctgcgcctcg tcgacgcgtt cgcgcgcctg ctgcgtgcgt
      781 gcctgcctta tttcgaattc gtcccgtgtt cgcgccattt cttcggtgca atgggtttgc
      841 tgatcggtcc attcctgaat ctgctgctgc aaatgcgtac gccggtcctg aagccgattg
      901 cgcgaatcga ccacatggtg tatctccgct tcgagccggc tgacctgggc gccggcttcg
      961 aacaatagac cctgagccgt atgcaccgca tcacccgcgg cgtagtgcgc ctgacggcgc
     1021 gactcgaccg ccgcctcgct ggcccgcaaa ccggcaatgg ctccttcgag ctgtgtttga
     1081 gcttgctcca gagccgcgag ccttgctcgc tgatcttcgc gcgcgccgct ttctttcaga
     1141 aaccacaacg cgctttgctt ctgccctccc tcgtcctgca acgcgcggta ttggcgcgcc
     1201 accgccgcct gggcgtcaag cttttcaagt tgtccggcaa gctcgcgcaa aatatcctcg
     1261 acgcgcgtca gattttcacg ggtatcgctc aagcggcttt cagtctcgcg acgccgctcc
     1321 ttatagcgcg acacacccgc cgcttcttca aggtaaatcc gcaattcttc ggggcgcgcc
     1381 tcgatcagac gatttatcat gccctggcca ataatggcgt agccgcgcgt accgagtccg
     1441 gtacctaaaa aaatgtcgtg aatatcacga cgccgcactt gttgaccgtt gacaaaatag
     1501 ctactggcgc cgtcgcgcgt aagcacgcgg cgcacagcaa tttcggcata aacgctccac
     1561 tgccccgcag cccggccctg agagttgtca aacaccaatt caaccgatgc ccgcgccgcc
     1621 ggtttgcgat tgcccgagcc attgaaaatg acgtcctgca tggattcgcc gcgcaattca
     1681 gaggctttgg tttcgcccaa aacccagcgc accgcgtcga taatattcga ttttccgcaa
     1741 ccattggggc cgaccacccc gaccaattgg ctaggcgtgg gaataaccgt cagttcaaca
     1801 aacgacttga aaccggcgag ttttatttgg gtcagacgca caataagcga gctatataaa
     1861 atggaaaatg ataagcaata ggcgctaccg gcccacgaac agccgcacca tcgctataaa
     1921 aaacagcccg ggcacgaggc cgaaggcgac atcgcgccgc ctttttgtat gataacgcac
     1981 gcccgatccg atcccctact aaaaaaggga ctttaaacgt taattgcaat gcgaatcaag
     2041 cggtaacgct atactcgtta acttattttg attaacactt tcgacttccg gtgagacacc
     2101 cgattgaaca aaggcttttt tctggtcatg acagcccagg cgctgtcgtc gttggcggat
     2161 aacgcattac tcatcgccgc catagcgctt atagctgaat tgcagggtcc cgactggatg
     2221 gcccccatga tgaaatggtg gttcgcgctg gcctatgtgg cgctggcggc ttttgtgggg
     2281 gcctttgccg attccttccc caagggccgc gtcatgttca tcaccaacgc catcaagctg
     2341 cttggctgcc tgatgatgtt cagctacgcc agctttggac tgacacattc cgaacagttg
     2401 ctgctggtct tttgcgccta cacactggtg ggtatcggcg cagcggccta ttcgccggcc
     2461 aaatatggga tcgtgaccga aatgctccca cccgatctgc tggtgaaggg aaatagctgg
     2521 atcgaaggcc tgacggtgct gtccattatc cttggcacgg tgttgggcgg cgtactcatc
     2581 agcccgagtg tctccctgat gctgctgagc cacccatgga tcagccactt cgtacacaac
     2641 ggcgttgagg ccgctatatt ccttatcgga tttgtttatt ttctggcggc cgtctgcaat
     2701 ttactgattc cgctcacggg ggtgagctat ccaaaacagc aaaccaatcc ggtccagctt
     2761 gtccgcacat tcaagagcta tgtccgcatc ttgtggcacg acaaacttgg tcaaatttct
     2821 cttgccgtca ccaccctgtt ctggggcgcg ggcgccacgc tgcaattcat cgtaatcgag
     2881 tggggcgcgc gacatctggg ctatcgtctt gaccaggcat ccattttgat gggcgtagca
     2941 gccgtcggca ccgtcatagg gtccgttgcg gcaggccggg ttccgctgaa acgcgccttg
     3001 tccgttctgc cagttggagt ccttatggga gtcgccgtgc tcttgatgcc gcttgtcacg
     3061 gaaacgtggg cagtttacag cctgctgctc attattggcg ccctgtccgg atttttcgtc
     3121 gtccccatga acgctttgtt gcagcaccgg ggccacgttc ttttatcggc cggtcattca
     3181 atcgccgtgc aaaacttcaa cgaacaattg aatattttgt tgatgctggc tgtgtacagc
     3241 gccatgcttt ggctgaactt gcccattaat tttattatcg ccatcttcgg cgtgctggtg
     3301 tccgcgctaa tgctcgtgtt catgcactgg aatcgcctga atcacgggaa agacccgggc
     3361 ctggaaaacc agatcggtca acatggccac ggccaggctt tgcactaaaa ctgcgcgccg
     3421 cccgtaaaaa tgcgccatcc gccagttcag tcgcaaagcg cggtgtacgc aagaatcagg
     3481 tcgctccatg cgccggcttt atactgcggg cgcgacaaca agtatgcagg atgataggtt
     3541 gccaccaatg gaattttcgc cccgctttca ctcagtaatt cgtgtacgca cccgcgcaat
     3601 tgttcaaagt ccgaatcgtc attcaaaaga gtctgagcgg ccagccggcc caaggtcaaa
     3661 atccgccgag gattgagcaa agcaatctga cgcctcaagt atggcaggca tgcagcgatt
     3721 tcagccggcc cgggcggccg attccccagc gggcggcact tgaccaggtt cgtgaaaaaa
     3781 acagacgaat cggctcgcac gcccaccgcc gccagcatgg cgtgcaatag cgttccggcc
     3841 gcaccctgaa acggcagccc cgcccggtca tcgctgtctc ccggcgcctc gccaatgatc
     3901 atccactgca gcgattcagt ggcacccgac ccgaaaacgg tccggctgcg tccggcataa
     3961 agagggcacg cttcacaaat cgccaccaac gaccgcaaac tttccaagtc gtgcggcaac
     4021 gagtcgctta ccggactcgc ggcgcgcaac aaagctttcg tgccaacagg ctgatcgatc
     4081 gccacgcgtt caggcactgt cgtaaccgta tcggcgccgg acgcgtcgaa tcgcccgagc
     4141 aaattcttgt caatgccgat ttcccgcaac cacgcacgct gtaccgcgct tagcctcagc
     4201 gctgcaggct cgtcgagatc aaccataggc caccttgtca acggccagct ttttttccat
     4261 cacccaggca tcctcgcgct cgttccagcg caacggataa taatccttgc gcacgctaag
     4321 gcgctgaaat ccgcgattct gataaaactc tacggcg
//
LOCUS       DATCXU010000063         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_300554,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000063 DATCXU010000000
VERSION     DATCXU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300554"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VIR04_01370"
     CDS             <1..595
                     /locus_tag="VIR04_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9459429.1"
                     /translation="SANNAAAQTAFIPLLTLGIPGNAVTALMIGAMMIHNIQPGPQVM
                     TSHPDLFWGLIVSMWVGNLMLIVLNLPLIGLWVKLLQVPYRILFPAILVFCTIGVYSL
                     NYNVFDIYMTAAFGVLGYLWTKLKCEGAPLLLGLVLGPMMEENFRRALLLGRGDYGTF
                     VTRPLSLSLLAVAAFLVVLVALPSIRKKREETFVEDD"
     gene            718..1641
                     /locus_tag="VIR04_01375"
     CDS             718..1641
                     /locus_tag="VIR04_01375"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9459430.1"
                     /translation="MASIGSKSYNPAPAQTVAFLGLGVMGFPMAGHLSRAGHHVTVYN
                     RTESKAQAWVKEFGGKAAKTPREAAQGASIVLCCVGNDDDLRSIVLGQDGALAGMAAN
                     SIFVDHTTASAEVARELFQAAKQKGVGFVDAPVSGGQAGAVNGVLTVMCGGEPAQFKS
                     VEPVVSTFARAVTLLGESGSGQLCKMINQICIAGMVQGLSEAIAFGQAAKLDMHQVLD
                     VISKGAAQSWQMENRGATMVDDKFDFGFAVDWMRKDLGLVLSEARRNGASVPVTALVD
                     QFYGDVQKMGGNRWDTSSLIKRLTSVGPAKK"
     gene            complement(1686..2243)
                     /locus_tag="VIR04_01380"
     CDS             complement(1686..2243)
                     /locus_tag="VIR04_01380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY9459431.1"
                     /translation="MLDREGYRPNVGIILVNQKNEVFWGKRIREHAWQFPQGGINYGE
                     NPVQAMYRELYEEVGLLPEHVRILGRTRDWLRYDVPGNFVRRDSRGLYKGQKQIWFLL
                     RMIGRDSDVSLRTNNHPEFDAWRWSEYWVPLDAVIEFKREVYTSALNELAVILFKRGQ
                     ETRYLRQRTQGRHNEHDKDHARTVG"
     gene            2309..>3978
                     /locus_tag="VIR04_01385"
     CDS             2309..>3978
                     /locus_tag="VIR04_01385"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY9459432.1"
                     /translation="MRASKYHINTLKEAPAEAEVTSHKLMTRAGMIRKVAGGIYTYMP
                     LGLKVIRKIEAIVRQEMDNAGAIELLMPVVQPAELWVESGRWDKYGAELLRIKDRHQR
                     DFVLQPTSEEVITDIARNEIHSWRQLPVNFYHIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRDETSAGASYDAMYDAYMRVFGRLGLEFRAVAADTGSIGGSRSHEFQVIADTGE
                     DLIVYNPGSQYAANIELAVAPQLLPERGPATEALTKTATPDAPKCEIVARQLGRPLTD
                     TVKSIVLATEPAPGAAKIWLLLLRGDHELNEVKTSKLSGFESGFRFATEDEIMATFGC
                     VPGYLGPINPLRPITIIADTTVANMSNFVCGANAEGFHYTGINWGRDLPEPAVADLRN
                     VVAGDPDPNGGKLAIQRGIEVGHIFFLGDKYSKALNATFLENDGKPALLQMGCYGIGI
                     SRIAAAAIEQGHDENGIIWPRAITPFEVVVCPMGWNKSETARNVALELYNELVNRGVD
                     VILDDRDIRPGVMFADWELIGVPLRITVGERGLKEGLVEILGRRDGATT"
BASE COUNT          914 a         1108 c         1124 g          832 t
ORIGIN      
        1 gtccgccaac aacgcggcgg cccaaaccgc tttcattccg ctgctcacat tgggcattcc
       61 gggcaatgcg gtgaccgccc tgatgatcgg cgcaatgatg attcacaaca tccagcccgg
      121 cccgcaggtc atgaccagcc accccgattt attctggggc ctgattgtgt cgatgtgggt
      181 cggcaacctg atgctaattg tcttgaatct gcctttgatc ggcttgtggg taaagctgtt
      241 gcaagtgccc tatcggatcc tgttcccggc gattctggtg ttttgcacca ttggcgtcta
      301 ttcgctgaac tacaacgtct tcgatatata catgaccgcg gcgtttggtg tgctgggcta
      361 cttgtggacc aaactaaaat gcgagggcgc gcctttgctt ctgggcctgg tcctggggcc
      421 gatgatggaa gaaaacttcc gaagagccct gctgctcggg cgcggcgact atggcacgtt
      481 cgtcacgcgg cctctgtcac tgtcgctgct ggcggtggcc gcatttttag tcgtgttggt
      541 cgcattgccc tccatcagga aaaaacgcga agaaacgttc gtcgaagatg actgaccggc
      601 gctaaatgtt cgctggaaaa ggcgcttcta caggcgcctt tttcttgccg cacaaaaaat
      661 ccggcgtaat gaagtattct tataagctga cttccctgtt tactggagac aaacaaaatg
      721 gcatcaatcg gcagcaaatc gtataacccg gcgcccgcgc aaaccgtcgc ttttctcgga
      781 ctgggcgtaa tgggattccc aatggccggc cacctgagcc gagcgggaca ccacgttacc
      841 gtctacaacc ggactgaaag caaagcgcag gcctgggtga aagaattcgg cggtaaagct
      901 gccaaaacgc ccagagaagc cgcgcagggc gccagtattg tgctgtgctg cgtgggcaac
      961 gacgacgatt tgcgcagcat cgtgctcggc caggacggag ccttggcggg tatggcggct
     1021 aattccattt tcgtggacca cacgaccgca tcggcggaag tggctcgcga gctctttcag
     1081 gcagcaaagc aaaaaggcgt cggttttgtc gacgcgcccg tatcgggcgg acaggccgga
     1141 gccgtcaacg gagtgcttac cgtcatgtgc ggcggcgaac cggcgcaatt caagtcggtc
     1201 gagcctgtcg tatcgacgtt cgcccgggca gtgaccttat tgggcgaatc aggttccggc
     1261 cagctttgca aaatgattaa ccaaatatgc atcgccggaa tggtgcaggg tttgtcggaa
     1321 gccattgcgt tcggccaggc ggccaaactg gacatgcatc aggtcctgga tgtgatcagt
     1381 aaaggcgctg cccaaagctg gcagatggaa aaccggggcg ccacaatggt ggatgacaaa
     1441 ttcgacttcg gctttgcggt cgactggatg cgtaaagatc ttggcctggt gcttagcgaa
     1501 gcccggcgca atggcgcgag cgtaccggta acggcgctgg ttgatcagtt ttacggcgac
     1561 gtgcagaaaa tgggcggcaa ccgctgggat acatccagtc tgatcaaacg gctgaccagc
     1621 gttgggccag ccaaaaaata agcgttcgcg gcccgatgct accagtgcat cgggcaccac
     1681 cacagctaac cgaccgtgcg cgcgtggtcc ttgtcgtgtt cgttgtggcg accctgcgtg
     1741 cgctgacgca aataacgagt ctcctgaccg cgcttgaata aaatcacggc caactcgttt
     1801 aacgccgacg tgtacacctc gcgcttgaat tcaatgactg catccagcgg cacccagtac
     1861 tcgctccagc gccatgcgtc gaactcgggg tgattgttgg tccgaagact gacatcggag
     1921 tcgcggccaa tcattctcaa caagaaccag atttgtttct ggcccttgta cagaccacgc
     1981 gaatcgcgcc gaacaaaatt gcccggtacg tcgtaacgca accaatcacg cgtacgtcct
     2041 aatatgcgaa catgctcggg caacaagccc acttcttcgt agagttcacg atacatggcc
     2101 tgcactgggt tctcgccata attaatgccg ccttgcggaa actgccaagc atgctcacgg
     2161 atacgtttgc cccagaaaac ctcgtttttt tgatttacga ggataatgcc gacattagga
     2221 cggtagccct cacgatctaa cataggccac ctcatagcta attcaataca attacgctcg
     2281 attatacgta tctgccggaa tatctaccat gcgcgcatcc aagtatcaca ttaatacgct
     2341 taaagaagct cccgcggaag cggaagtgac cagccataaa ctcatgaccc gggctggcat
     2401 gatcagaaaa gtagcgggag gcatctatac gtatatgcca ttgggcctga aggtgataag
     2461 gaaaatcgaa gcgatcgttc gtcaggaaat ggataacgcc ggcgccatag agctcttgat
     2521 gccggtggtc caaccggccg agctttgggt cgagtcaggc cgttgggaca aatacggggc
     2581 ggagctgctg cgcattaaag atcggcacca gcgcgacttc gtcctgcagc ctacgtcaga
     2641 agaagtcatc accgatattg cgcgcaacga gatacatagc tggcgccaac tgcccgtcaa
     2701 tttctatcac attcagacca aattccgtga tgaacggcgg ccgcgcttcg gacttatgcg
     2761 cggacgcgaa ttcaccatga aagacgccta ttcatttgac cgcgacgaaa catcggcggg
     2821 ggccagctat gacgccatgt acgacgcata catgcgggta ttcggccggc tgggactgga
     2881 atttcgcgcg gttgccgccg ataccggttc aatcggtgga tcgcgcagcc acgaattcca
     2941 ggttattgcc gataccggcg aagaccttat tgtgtataac cccggatcgc aatacgcagc
     3001 caatatcgag ctggccgtcg cgccgcaatt attgcctgag cgcggcccgg ccaccgaagc
     3061 gctaacgaag accgctacgc cagacgcgcc aaaatgcgaa attgtggcgc gtcaacttgg
     3121 ccgccccttg accgacactg tcaagtctat cgtactggcg acagagccgg cgcccggcgc
     3181 cgctaaaata tggctcctgc tgctgcgcgg tgaccacgag ctgaatgaag ttaaaacaag
     3241 taaactttcg ggttttgaaa gcggattccg tttcgccacc gaggatgaaa taatggccac
     3301 gttcgggtgc gtccctggat acctgggacc gataaatcct ttgcgcccca tcacaatcat
     3361 cgccgatacc actgtggcca acatgagcaa ctttgtgtgc ggcgccaatg cggaaggatt
     3421 tcactacact ggcatcaact ggggccgcga tctgcccgaa cctgccgtgg cagacctgcg
     3481 caacgtcgtg gcgggcgatc cggatcccaa tggcgggaag ctcgccatcc agcgtggcat
     3541 agaggtcggc cacatcttct ttctgggcga caaatattca aaagccctga acgcaacgtt
     3601 ccttgaaaat gacggcaaac cggctttgtt gcagatgggg tgttacggca taggcatcag
     3661 ccgtatcgcg gcggctgcaa ttgagcaagg ccacgatgaa aatggcatta tctggccccg
     3721 ggccatcaca ccgttcgagg tggtcgtgtg cccgatggga tggaacaaaa gtgaaacagc
     3781 tcgcaacgtc gcgctggaac tctataacga attggttaat cgtggcgtcg acgtcatcct
     3841 tgatgacaga gacatccgtc ccggcgttat gtttgccgac tgggaactta taggcgtgcc
     3901 gctgcgcatc accgttggcg aacgtggcct gaaagaagga ctggtcgaaa ttctgggacg
     3961 gcgcgacggc gccacaac
//
LOCUS       DATCXU010000064         7271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_457170,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000064 DATCXU010000000
VERSION     DATCXU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457170"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VIR04_01390"
     CDS             <1..267
                     /locus_tag="VIR04_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9459433.1"
                     /translation="APEAAGAAVNPAGEKLYKTVCFACHTAGVAGAPKFGNKADWAPF
                     VATGIDAMVKNAISGVGAMPPRGGSQATDAEIHEAVEYMVQAVK"
     gene            complement(324..1859)
                     /locus_tag="VIR04_01395"
     CDS             complement(324..1859)
                     /locus_tag="VIR04_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY9459434.1"
                     /translation="MKSIKTIGIVGAGAMGRGIAQIAAQAGLTVCLFDVNPQATTAAR
                     DHLEQLWARLSAKGKITAELAAGSLQRIQVCADLKELIQADLVVEAVVERLDVKRDLF
                     RQLENCVAPDCILASNTSSLSITAIAAGCAHPERVAGYHFFNPVALMKVVEVIDGLRT
                     ASQTGDALMDLARRMGHTAVRAKDMPGFIVNHAGRGMSIEGVRLAQESVASFSQIDAI
                     MREQAGFRMGPFELMDLTGLDVSHPVMESIYRQFYDEPRFRPSPLTAVRYAGGMFGRK
                     TGQGFYRYVEGQKQVPAEPAAPALPADLKTWVAPYQPVGHKRAAALLRQLGAHVIAAE
                     APPPDALIVVTPFGEDTATVVAMHQLDGARTVALDTFFGLEQGKRRVLMCSAATQPSW
                     RDAAHALFGVDGTAVSVINDSPGFVAQRVVATIVNVASDIAQQAIATPEDIDLAVSLG
                     LGYPLHGPLTMGNTLGAAHILEILRAMHRVTGDARYRASLWLQRRVQLGLPLQTEPMC
                     KAQ"
     gene            complement(1866..3101)
                     /locus_tag="VIR04_01400"
     CDS             complement(1866..3101)
                     /locus_tag="VIR04_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY9459435.1"
                     /translation="MSLRPAPLTGIRVLDLTRVLAGPWCTQNLADLGAEVIKIERPGS
                     GDDTRSWGPPYLKDEQNYNTTEAAYYLSANRNKQSVALDIASERGAGLVRELARQSDI
                     MVENFKVGGLRKYGLDYESIKTVNPALIYCSITGFGQTGPYANRPGYDFMIQGMGGLM
                     SITGERDDLPGGGPQKAGVAVADLMTGMYSTVAILAALHERASSGLGQYIDMALFDCQ
                     VAMLANQNLNYLTSGVAPKRAGNAHQNLVPYQVFAAADGHLIVAVGNNTQFAAYCNVI
                     GRPDLPVDVRFKTNPDRVVNRDLLVPILIDVMKTRARDDWLKALERAGVPAGPINTID
                     QVYQDPQLLSRNMRLDLPHASAGHAPVAASPMKLSGSPVVYRHAAPLLGEHTRQVLRE
                     RLGLSDEEIEQLSSAKERG"
     gene            3271..3504
                     /locus_tag="VIR04_01405"
     CDS             3271..3504
                     /locus_tag="VIR04_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459436.1"
                     /translation="MSYITYNQYARVTDAMERQQMRDALSCGEPFSLFEEIKRAYSVS
                     KKAIIATLDFIVDVNQLMSEAREKALRLGDSRW"
     gene            complement(3512..5590)
                     /locus_tag="VIR04_01410"
     CDS             complement(3512..5590)
                     /locus_tag="VIR04_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY9459437.1"
                     /translation="MNLPVGHTVAADLNAVQREAVLYLDGPCLVLAGAGSGKTRVITQ
                     KIAYLLRECGYSGRQIVALTFTNKAAREMDERVKKLVDPKLAKGLTISTFHSLGLRFL
                     REEARHAGLKPQFSILDANDALAIIQDLLATTDKGRLKAVQQVISLWKNALMDPDAAE
                     RAAATPSEVEAARIYRSYSATLTAYQSVDFDDLIRLPALLLESDPELRDRWQSRVHYL
                     LVDEYQDTNLCQYRLVQWLAGQRAMLTVVGDDDQAIYAWRGATVENLARLTTDYPALK
                     VVKLEQNYRSVQRILSAANQVIKKNQNLYTKKLWSDLGTGEPIQVTPMDNEQVEAESI
                     AVRISAARFERRAQWRDFAVLYRSNQQARVIEQALRTLHIPYTISGGQSFFEKPEIRD
                     ILAYLRLIANNDDDPAFIRAVTTPRRGIGQASLQSLGQFAAERQISLFNAIFEIGSTD
                     RLGVRQLEPLQVFGAFIRRIQARADGKRGPGASRTAPPEPAAEILDELLHAIQYERFL
                     YDTLDERPAQMRWQNVLELVNWLKRKAQEDALDLFDLAQHVALVTLLERSDDDEPDAV
                     KLSTLHASKGLEYRHVYLVGVEEGLLPHMGRGEEDGDSARAAESRVLRIQEERRLMYV
                     GITRAQRSLHLTWCKKRRRAREDVVREPSRFIAEMGLAEDKLEEDPETRALSPKERLQ
                     MLKNLLGKAG"
     gene            complement(5605..>7271)
                     /locus_tag="VIR04_01415"
     CDS             complement(5605..>7271)
                     /locus_tag="VIR04_01415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY9459438.1"
                     /translation="RQVKPDQAPALNAEQTQAAQAIGALEGHKVVLLHGVTGSGKTEV
                     YLRAAQAVLARGRQVLFMVPEINLTPQLEQSLRGRLASMTGMNTLAVLHSGLADGERL
                     RSWLDVQRGRAQILLGTRLAIFAPIPNLGLIIVDEEHDTSYKQQEGLRYSARDLAVWR
                     AHDRRVPIILGSATPSLETWHHAQRGHYLKLSLPHRAHAVELPQIRLVDTRRLTLQQG
                     FSPQLITALNERLDRGEQSLIFLNRRGYAPVLTCASCGWVTQCPRCTAYTVLHRASGQ
                     RNYLQCHHCGYQARVPNACPDCGDQDLRPMGRGTQRVEETLAQLFPQARIARIDADST
                     RLKGSAAALFAKVHAGEVDVLVGTQMVAKGHDFTNLGLVGVLNADAMLFAQDFRAPER
                     LFAQLMQVAGRAGRHVGGGEVIIQTDYPEQPVYQALVRHDYAGFAQYGLAERETIGLP
                     PYAFQALLMAEAPKLKTALDFLTRARALPGEQPENYPSAALITLYDPVPLRVVRVANI
                     ERAQLLVESRSRPALQAFLAVWSAALPALASRPRVRCQLEVDPLEI"
BASE COUNT         1607 a         2224 c         1949 g         1491 t
ORIGIN      
        1 gcccctgagg cggccggagc agctgtgaac cccgctgggg aaaagcttta taaaacggtg
       61 tgcttcgcct gccacacagc cggcgtcgcc ggcgccccca aatttggcaa taaggccgac
      121 tgggctcctt tcgtagccac cggcatcgac gccatggtga aaaacgcaat cagcggcgtc
      181 ggcgcaatgc ctcctcgtgg cggctcgcaa gctaccgatg ctgaaataca cgaggcggtc
      241 gaatacatgg tgcaggcagt caaataattc tttaaagcaa gttcatgctc ccccggcctg
      301 gtccggggga ttttttttac ggtttattgc gctttacaca tgggttcagt ctgcagcggt
      361 aagcccaact gaacccggcg ctgcaaccac aagctcgcgc ggtaacgtgc atcgcccgta
      421 acacgatgca ttgcgcgcag tatttccagt atgtgcgccg cgcccagtgt attgcccatg
      481 gtcaatggcc catgcaaagg atatcccagc cccagcgaca ccgccaggtc gatatcttcg
      541 ggagtggcga tggcctgttg cgcaatatcg ctggccacat tgacgatcgt cgccaccacg
      601 cgttgcgcca caaagcccgg tgaatcatta attaccgaca cggcagtccc gtcgacgcca
      661 aacagggcat gagcggcgtc gcgccacgac ggttgtgtcg cggccgaaca catcaacacc
      721 cggcgtttgc cctgctccag accaaaaaac gtatccagtg ccacggtgcg cgcgccatcg
      781 agctgatgca tggcgacaac tgtcgccgta tcttcaccga agggcgtaac tacaatcagg
      841 gcgtccggtg gcggcgcctc ggcggcgatc acatgcgcac ccagttgcct tagcaaggcg
      901 gcggcgcgct tgtgcccgac aggctgataa ggcgcaaccc acgtctttaa atcggccggt
      961 aaggccggag ccgcaggctc ggccggaacc tgcttttggc cttccacata acggtagaat
     1021 ccctggccgg ttttccggcc aaacatgcca cccgcataac gcacggccgt cagcggagac
     1081 gggcgaaacc ggggttcatc gtagaactgc cgatagatcg attccatcac cggatgcgac
     1141 acgtccagcc cggtcaggtc cattaattca aatggcccca tgcggaaacc cgcttgttct
     1201 cgcatgatgg cgtcgatctg ggaaaatgac gcgaccgatt cttgcgccag tcgaacgcct
     1261 tcaatgctca tgccccggcc ggcgtggttc acaataaaac cgggcatatc tttagcccgc
     1321 accgccgtat gccccatgcg gcgcgccaaa tccataagcg catcgccagt ttgcgaagcc
     1381 gtacgcaatc cgtcgatcac ctcgaccact ttcatcaggg caactggatt gaagaagtgg
     1441 tagcccgcaa cgcgttcggg atgggcgcat cccgccgcaa tcgcagtaat cgatagcgac
     1501 gatgtattgg acgccagaat gcaatcgggc gccacgcaat tttcaagctg cctgaacaag
     1561 tcgcgcttga cgtccagccg ctccaccacc gcctcgacca ccagatcggc ctggatcagt
     1621 tctttcaaat cggcgcaaac ttgaattctc tgcaacgatc cggccgccaa ttcagcggta
     1681 atttttcctt tcgccgacaa gcgcgcccaa agctgctcca gatgatcgcg cgccgccgtt
     1741 gtggcctgcg ggttcacatc gaacaagcac actgtcaaac ccgcctgcgc ggcaatctgc
     1801 gcgatcccgc ggcccatggc gcctgcgcca acaatgccga tcgttttaat gcttttcatt
     1861 gcagcctacc ctcgttcttt tgcggatgac aattgctcaa tctcttcatc tgacaagccc
     1921 agacgttcgc gcaatacctg cctggtgtgc tcgcccagca atggggccgc gtgtcgataa
     1981 acaaccgggc tacccgaaag cttcatcggg ctggccgcca cgggcgcatg gcccgcggat
     2041 gcatgcggca aatccagcct catattgcgc gatagcaact gcgggtcttg atagacctga
     2101 tcgatggtat tgatgggacc ggcgggaact cctgcgcgct caagagcctt gagccagtcg
     2161 tcgcgcgcac gcgtcttcat gacgtcaatc aatataggca ccagcaagtc gcgattgaca
     2221 acgcggtcgg ggttggtttt aaaacggacg tccaccggca gatcgggacg gccgatgaca
     2281 ttgcaataag cggcaaattg ggtgttgttg cccactgcca cgatcagatg tccgtcagcc
     2341 gcggcaaaaa cctgataagg caccagattt tgatgcgcat tgccggcccg cttgggcgct
     2401 acgcccgatg tcaagtaatt aaggttttga tttgccagca tcgcgacctg acaatcaaac
     2461 agcgccatat caatatactg gcccagtccg ctggatgcgc gctcgtgcag agccgccaga
     2521 attgccacag tactgtacat tcccgtcatc agatcagcca ccgccacgcc cgctttttgc
     2581 ggacctccgc caggcaaatc atcacgctcg ccggtgatgc tcattaatcc gcccattccc
     2641 tgaatcatga aatcgtagcc agggcggttc gcgtagggtc ctgtttgccc aaaccctgtg
     2701 attgaacagt agatgagagc gggattaacc gtcttgatgc tttcgtaatc aagaccgtac
     2761 ttgcgcagac cgccaacctt gaagttttca accatgatgt cgctttgcct ggccagttcg
     2821 cgcaccaacc cggcgccgcg ttcgcttgca atatcgagcg ccacggactg cttgttgcgg
     2881 ttggctgaca gatagtacgc cgcctcggtc gtgttgtagt tctgttcatc tttaagatag
     2941 ggaggccccc aggagcgggt gtcatcgccc gaaccgggcc gttctatttt gattacctca
     3001 gctcccagat ccgccaggtt ttgcgtgcac catggcccgg ccagaacacg agtgagatca
     3061 agtacacgaa taccagtaag gggagcagga cgtagagaca tatcgacaaa gatgaataaa
     3121 aaataaacat tgtgcccgaa gtgaggcggc tttcagaaaa aaaacaacag aaacgtcctc
     3181 tgcaatgaaa tattagggta aacccttgca tgaaattaag ggtttaccct atactaaatt
     3241 cattgattca ctgtttggaa agaatttcaa atgtcatata ttacttataa ccagtacgca
     3301 cgcgtgaccg atgcaatgga acgccagcaa atgcgcgacg ctctcagttg cggcgagcct
     3361 ttttccttgt tcgaagaaat caagcgcgct tattcagtat cgaagaaagc aatcatagcc
     3421 acgctcgatt tcattgttga tgttaaccaa ttgatgtccg aagcgcgtga aaaggcgctt
     3481 cgcctgggtg attcccggtg gtaattgcgt atcaacctgc cttgccaagc aggtttttta
     3541 acatctgaag gcgctccttg gggcttaatg cccgggtttc gggatcttct tccagcttgt
     3601 cttccgcaag acccatttcc gcaatgaagc gcgagggctc tcgcaccaca tcctcacgcg
     3661 cacgccgccg cttcttgcac cacgtcagat gcaggctgcg ttgcgcgcgg gtaatgccca
     3721 catacatcag gcgacgctct tcctgtatgc gaagcacacg gctttcagcc gccctggcgg
     3781 aatcgccatc ttcctcgccg cgccccatat ggggcagtaa tccctcttcg acacccacca
     3841 aataaacgtg gcgatattcc agcccttttg aagcgtgcag cgtcgataac ttgaccgcgt
     3901 cgggttcatc gtcgtcgcta cgttccagca aggtgaccag cgccacgtgc tgcgccaaat
     3961 cgaataaatc gagcgcgtct tcctgcgcct tgcgtttcaa ccaattgacc agttccagaa
     4021 cgttttgcca acgcatctgc gccggccgtt cgtcgagcgt atcgtacaaa aagcgttcgt
     4081 actgaatggc gtgcaacagc tcgtccagga tctcggccgc tggctcgggc ggcgccgttc
     4141 tcgaggcccc cgggccacgt ttgccgtccg cgcgggcttg tatgcgccga ataaatgctc
     4201 cgaatacctg caacggctca agctgcctga cgcccagacg atcagtcgag ccgatctcaa
     4261 aaatagcatt gaacagtgaa atctgacgct cggcggcaaa ctggcccagg gattgcaacg
     4321 acgcctgacc gatcccgcgg cgtggcgtgg ttacagcgcg aatgaatgcc gggtcatcgt
     4381 cgttattggc gatcaagcga agataagcca gaatatcccg gatctccggc ttttcaaaaa
     4441 agctctgacc acccgaaatg gtgtagggaa tatgcaaagt tcgcagcgcc tgctcgatga
     4501 cgcgcgcctg ctgattgctc cgatacagca ccgcaaaatc gcgccattgg gcacgacgtt
     4561 caaagcgcgc agccgaaata cgaacggcaa tcgactcggc ctcgacctgc tcgttatcca
     4621 taggggtcac ttgtatgggc tctcccgttc ccagatcgga ccacagcttc ttggtataaa
     4681 gattctggtt ctttttgata acctgatttg ctgcggacaa aatgcgctgc accgaccggt
     4741 aattctgctc gagcttaacg actttcagcg ccgggtaatc cgttgtcagg cgagccaggt
     4801 tttcaaccgt ggccccgcgc cacgcataaa tcgcctgatc gtcgtcgccc acaactgtca
     4861 acatggcgcg ctgccccgcc agccactgca ccagacgata ctggcacaaa ttcgtgtcct
     4921 gatactcatc gacaagcaaa tagtgcacgc ggctttgcca gcgatcgcgc agctccgggt
     4981 cgctttccag caatagcgcg ggcagacgaa tcaaatcgtc gaaatcgaca gactgatagg
     5041 ccgtcaatgt tgcgctgtag ctgcgataaa tccgggctgc ttcaacctcg ctgggagtcg
     5101 ccgccgcacg ctcggccgca tccggatcca taagggcatt cttccataaa gaaattacct
     5161 gctgcacggc tttcaaccgg cctttgtccg tcgtggcaag caaatcttga ataatcgcca
     5221 gagcatcgtt ggcgtccaga atcgaaaact gaggtttcaa tccggcatgc cgcgcctctt
     5281 cacgcaggaa tcgcagccca agcgagtgga atgtactgat agtcagacct ttcgccaatt
     5341 tagggtcgac aagcttttta actcgctcgt ccatttcgcg cgccgcttta ttggtaaacg
     5401 tcagagcaac aatctggcgc ccggaatagc cgcattcacg tagtaaatag gcgatttttt
     5461 gcgtgatcac ccgcgtcttg ccgctacccg cgcccgccag caccagacat ggcccatcca
     5521 ggtacagtac agcctcgcgt tgcacggcgt tcaaatcggc ggccacggtg tggccaacgg
     5581 gaagattcat tgtggttggc ctgcttaaat ttccagaggg tcgacctcga gctggcatcg
     5641 cacccgcgga cgactggcga gcgccggcaa cgccgccgac cacactgcca gaaacgcctg
     5701 caatgcgggg cggcttcggc tttcgaccag caactgggcg cgctcgatat tggccacacg
     5761 tacgacgcgc agcggcaccg ggtcgtacaa agtaataagc gccgcactgg gatagttttc
     5821 gggctgctcg ccgggcagcg cgcgagcgcg cgtcagaaaa tcaagcgctg ttttaagctt
     5881 gggcgcttcg gccatcaaaa gcgcctgaaa agcgtaggga ggcaagccaa tggtctcgcg
     5941 ttcggccagt ccgtactggg caaagccagc gtaatcatga cggaccagcg cctgataaac
     6001 cggctgttcc ggataatctg tctggataat gacctcgccg cccccgacat ggcgtcccgc
     6061 cctccctgcc acttgcatca attgcgcaaa caggcgctcg ggcgcccgaa agtcttgtgc
     6121 aaaaagcatg gcgtcggcgt tcaacacgcc aaccagcccc aaatttgtaa agtcgtgccc
     6181 tttggcgacc atctgcgtgc ccaccaatac gtcgacctcg cccgcgtgca ccttggcaaa
     6241 caaagcggcg gcgctgcctt tcaagcgtgt gctatccgca tcaatgcgag caatgcgtgc
     6301 ttgcgggaac agttgggcca gcgtttcttc gacgcgttgc gtgccgcggc ccatgggccg
     6361 caaatcttga tcgccgcaat cggggcaggc atttggcacc cgcgcctgat aaccgcagtg
     6421 atgacactgc aaataattgc gctgcccact cgcgcggtgc aacacggtat aggcggtgca
     6481 acgcggacac tgagtcaccc aaccgcagga ggcgcacgtt aataccgggg cataaccacg
     6541 ccggttcaga aaaataagcg actgctcgcc acgatccagg cgctcattta aagcggtgat
     6601 caattgcgga gaaaagcctt gctgcagggt cagccgccgc gtatccacca accgaatttg
     6661 aggaagttcg acggcgtgcg cccgatgcgg caaggatagt ttcagatagt gtccgcgctg
     6721 cgcatgatgc caggtttcga gcgacggtgt cgccgacccc agaataatcg gaacccgcct
     6781 gtcgtgagcg cgccacacag ccagatcgcg ggcggaatat cgcaatccct cttgttgttt
     6841 ataagaggtg tcgtgctctt cgtcgacgat aatcaagcca agattcggaa tgggcgcgaa
     6901 aatcgccagg cgcgtgccca acaggatttg cgcccggccg cgctggacat ccagccacga
     6961 acgcaagcgt tcgccatcgg ctaaaccgct gtgcaacacg gccagcgtgt tcatccctgt
     7021 catcgacgcc aaacgccctc gtaatgattg ctccaactga ggcgtcaggt tgatttcggg
     7081 caccatgaac aacacctgcc gcccccgcgc caggaccgct tgcgcagcgc gcaaatatac
     7141 ttccgtcttg ccgctacccg tgaccccatg gagcaaaacg actttatgac cttccaatgc
     7201 gccaatggcc tgagccgcct gcgtttgctc ggcatttagc gcgggggcct gatcgggttt
     7261 cacctgacgc c
//
LOCUS       DATCXU010000065         5363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_540245,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000065 DATCXU010000000
VERSION     DATCXU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540245"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="otnK"
                     /locus_tag="VIR04_01420"
     CDS             <1..962
                     /gene="otnK"
                     /locus_tag="VIR04_01420"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEY9459439.1"
                     /translation="MSALDTPFTIACPAFPENKRTLFQGYLFVGDVPLAESGMRHHPL
                     TPMVDSNLVRVLQRQTTRAVGLIRYDVVAAGAAAIRERMKQLQTEGVHIAIVDALDND
                     DLIQIGAACSEMPLITAGSGVALGLPQNFRSMGLLKPHAAADDFPAVGGLRAVICGSC
                     SQATQAQLADLIAHGTPSYAIDPLRLGLGADVAAEALAWAAPLIQNGPVVIYSTACAQ
                     VVESVQRSLGVASAGALVEKVLARIAQGLVALGVRRLVVAGGETSGAIVTALKVRRLR
                     IGPQIDPGVPWTVSMDGVPIALALKSGNFGSTDFFTKAWGFVS"
     gene            1161..2147
                     /locus_tag="VIR04_01425"
     CDS             1161..2147
                     /locus_tag="VIR04_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459440.1"
                     /translation="MNIGKISGLAGAVAALCLGFISAGVSAQTSYPTRPITLVVPYGS
                     GGSADVYARVLGSRLQKILGQPFIVEDKPGAGAVIGTSYVARSAPDGYTLLVISNTLT
                     ANETILKHKPYSLMGDFVAVAPINETSLVLVLSQKTKADSVKSLIALAKQNPGKLDYA
                     SSGIGTPYHLAGELFKTMAGITAQHIPYKSSGQARTGVAGGEVDFMFDAISTMKGLIA
                     SGKVRPIATTGTERSPVLPDVPTVIEAGVPGYTANLWLGVIAPKGTPPEIVNKLNAAI
                     NQVITTPEIKADWAKDGTTPMVMTPAQYTKYLEQDIVKLGKIAKEAHMVVEN"
     gene            2150..2893
                     /locus_tag="VIR04_01430"
     CDS             2150..2893
                     /locus_tag="VIR04_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY9459441.1"
                     /translation="MTGQSPVKLMLLSGGAAFALIEGIRNKFEADNSCRIEGEFAAVG
                     FTKDKLLAGAACDAIIVSRKLIDQLSGSRVVDSNTVRDLGRVSTGIAVKTGQEKIRIG
                     SAEDLERALSGADKIFVPNMGKSTAGLHMKTVFDKLDLFDRIRPKISEYSGGAAAMEA
                     LAIEPAGRCIGFTQVTEILCTRGVDLIGKLPSGFELDTTYSIAIPAVSRQTRLAGKLI
                     DLLTDSSLRKLKAATGFGAEIAPGARAAT"
     gene            3172..3822
                     /gene="ribA"
                     /locus_tag="VIR04_01435"
     CDS             3172..3822
                     /gene="ribA"
                     /locus_tag="VIR04_01435"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY9459442.1"
                     /translation="MQPLNGAGVGALADTRINYIASARLPTPWAVFRVHAFVDPTTGK
                     EHIALTLGEFGDGAPALARVHSECLTGDALFSQRCDCGAQLETALKRIAAEGRGIVLY
                     LRQEGRGIGLVNKIRAYELQDQGADTVEANEKLGFAADLRHYDLCKPMLDHFKIAQLR
                     LMTNNPRKVRALEQLGVRITERISLQVNRNPYNEKYLSTKAAKLGHWLKEHLAEKE"
     gene            3841..4068
                     /locus_tag="VIR04_01440"
     CDS             3841..4068
                     /locus_tag="VIR04_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY9459443.1"
                     /translation="MVPLYTPKSESEAAVIASLLHAYGVSFFMRGAAFSTMYPGSMTS
                     SLNEQTLMVRDDHVELAKGLLHSFLTGQEAI"
     gene            4238..5272
                     /gene="lipA"
                     /locus_tag="VIR04_01445"
     CDS             4238..5272
                     /gene="lipA"
                     /locus_tag="VIR04_01445"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY9459444.1"
                     /translation="MSNDATLAQPTVKSRLPSGEAVVSRPRPGSKFVSPQGTRAVKDG
                     IRPNDKSDVPDIGPKPAWLRVRLPAGAAYENVRNIVKTHKLNTVCAESKCPNIAECWG
                     RGTATLMLMGSVCTRACKFCSVSTGNPHGWLDKKEPENVADAVALMNLRYVVLTSVDR
                     DDLFDGGASHYAACVRAIHARSPQTAVEALTPDFDGNVKSVAKVLDAGLVTFAQNIET
                     VERLTHVVRDVRAGYRKTLNVLEFAKGYAPKTHTKTSMMLGLGETDDEIDRTLDDIRA
                     VGVDVVTLGQYMRPTQNHLPVQRFVTPEQFKAYRELALSKGFLEVVSGPLVRSSYRAE
                     RVLNHDNVGL"
BASE COUNT         1153 a         1398 c         1565 g         1247 t
ORIGIN      
        1 tcatgagcgc gcttgacacg ccgttcacga ttgcatgccc cgcgtttcca gagaataaac
       61 gcacgctgtt tcaaggctat ttatttgtcg gcgacgtgcc gcttgctgaa agcggcatgc
      121 gtcatcatcc gcttaccccg atggtggatt ccaatctggt ccgtgtattg caacggcaga
      181 cgactcgtgc cgtcggcctg atccgctacg acgtcgtggc agccggggcg gcggcgatcc
      241 gtgaaagaat gaagcaattg caaacagaag gggtgcatat tgcgatcgtt gatgcgcttg
      301 ataacgacga tttgatccag atcggtgcgg cgtgttccga aatgccgctg atcacggccg
      361 gctcgggcgt tgccttgggc ttgccgcaaa atttccgcag catgggtctg ctcaagccgc
      421 atgccgcggc ggacgatttc cccgccgttg gcgggttgcg cgcggtgatc tgcggcagtt
      481 gttctcaggc gacgcaggcg cagttggccg accttatcgc ccacggaact ccgtcgtacg
      541 cgatcgaccc gcttcggctc ggacttggcg ccgatgttgc agcggaagcg cttgcatggg
      601 cggcccctct catacagaac ggaccggtag tgatttattc cacagcttgc gcgcaagttg
      661 tggaatcggt acaacgatcg ctgggtgttg caagtgccgg cgcgttagtt gaaaaggttc
      721 tggcacgcat tgcacaaggg ttggtggcgc taggtgtgcg ccggctggtc gttgcgggtg
      781 gcgaaacgtc tggcgcaatt gtcaccgcac tgaaggtccg ccgcctgcgt atcggccctc
      841 aaattgaccc cggggttccc tggacggtaa gtatggacgg ggttcctatt gcgctggcat
      901 tgaaatcggg gaatttcggc agtaccgatt ttttcaccaa agcatggggt tttgtttcct
      961 gaacggagtt cgttcgtatg agtggcggcc attaatttcg cttcacgcat ttgctcacat
     1021 tcagcttgtt ctcagaaaat ttttttgcat ccgaatctta ttgttgttac tctttttgcg
     1081 gatgatcggc accatggctt ttagaacagc cgtcaatgcg gcgcgtgcct tcagaatctg
     1141 acgcaaacta ggagacaatt ttgaatatcg gaaaaatttc tgggctcgca ggcgcagtgg
     1201 cggcgttatg cctgggcttc ataagcgccg gggtgtcggc gcaaaccagt tatcccactc
     1261 gccccataac actggtagtc ccttatggtt cgggaggatc cgccgatgtc tatgcgcgcg
     1321 ttttaggttc caggctgcaa aaaatactgg ggcagccctt tatcgtcgag gacaagcccg
     1381 gcgctggcgc cgttattgga acgtcctacg tggcgcgatc ggcgcccgat ggctacacgc
     1441 tgcttgtcat ttccaatacc ctgaccgcta acgaaaccat cctgaagcac aagccataca
     1501 gcctgatggg ggattttgtc gcggtggctc caattaatga aaccagtctg gtgctggtgc
     1561 taagccaaaa gaccaaagca gatagcgtca agagcttgat cgctttggcc aaacaaaatc
     1621 ccggcaagct cgactatgca agttccggca ttggcacgcc ctatcatttg gcgggcgagt
     1681 tattcaaaac gatggcgggc atcactgctc aacacatccc ttacaagagc agcggccagg
     1741 cgcgcacagg ggtggcgggt ggcgaagtcg atttcatgtt tgacgccatc tcaacgatga
     1801 aaggccttat tgcaagcgga aaagttcgcc cgattgccac gaccggcacg gaaagatcgc
     1861 ccgttctgcc cgatgtgccg acagtaattg aagccggcgt gcctggttat acagccaatc
     1921 tttggctggg agtaattgcc cccaagggaa cgccgcccga gatagtaaat aagcttaatg
     1981 cggcaatcaa tcaggttatc accacgcccg agatcaaggc ggattgggcc aaggacggaa
     2041 ccacgcctat ggtgatgact cccgcccaat atacgaaata cctggaacaa gatatcgtta
     2101 aattgggcaa gattgccaaa gaggctcata tggtggtgga gaattaatta tgactggaca
     2161 gagcccggtg aaattgatgt tgctaagcgg aggagcggca ttcgcgttaa tagaaggaat
     2221 caggaataag ttcgaggccg acaattcgtg ccgcattgaa ggcgaatttg ccgcggtcgg
     2281 atttacgaaa gacaagctgc tggcgggcgc tgcttgcgac gcgataatag tgtctcgcaa
     2341 attgattgat caattgtcag gcagtcgcgt tgttgattcg aacaccgtcc gcgatctggg
     2401 gcgggtaagc acgggaatag ccgtcaagac aggtcaggaa aaaattcgga taggctcggc
     2461 agaagacctt gaacgggcac tatcgggcgc ggataagatt tttgtgccca atatggggaa
     2521 gtccacggcg ggcttgcaca tgaaaacagt tttcgacaaa ttggacttat ttgaccgcat
     2581 tcggccaaaa atttccgaat attcaggcgg ggcggccgcc atggaggccc ttgccattga
     2641 gccggccggc cgttgcatcg gttttaccca ggtgactgaa attctttgca cccgcggcgt
     2701 cgatctgatt ggcaaactgc cgtcggggtt tgagctggat actacttatt ccatcgccat
     2761 tcccgctgtt tcgagacaga ccaggctagc gggaaagctg atcgatctat tgaccgactc
     2821 gtcgttgcgc aaactgaaag ccgctacggg gtttggagct gaaatcgcgc cgggggcacg
     2881 agcggccaca tgatcaggca tgcttggcgg cttctttttt aagccgcttg gcctctttct
     2941 tttcttttgg tgttttggtg ggttgcttct tttccatctt tttgctgtcc tggcccttgc
     3001 tcatggtttc ctcctgaagg ttgaaaaatg atgctcaatt tggcagcctt cgtagtatag
     3061 gccatattga aagttttcca agcgccacgc gcctgccagg cgatgtctgt ggtctcttgt
     3121 tgtcggccga agggtgttaa tctggtgcta ttcatttcct gaggcttttt tatgcagcca
     3181 ttgaacggcg ccggcgttgg tgccctggcc gataccagga tcaactatat tgcgtcggcc
     3241 aggttgccga ctccatgggc tgtatttcgg gttcatgcgt ttgttgatcc gacaacaggc
     3301 aaagagcaca ttgcgctcac attgggcgag ttcggcgatg gcgcgccggc acttgctcgc
     3361 gtccattccg aatgtttgac aggcgatgcc ctgttcagcc agcgttgcga ttgcggagcg
     3421 cagcttgaga cagcgctcaa gcgcatagca gcggagggac gcggcatcgt gctttatttg
     3481 cgtcaggaag gccgtgggat tgggctggtg aacaaaattc gcgcatacga gctgcaagat
     3541 cagggcgctg atacggtcga ggctaacgaa aaactgggtt ttgccgccga cctgcgccac
     3601 tacgatttgt gcaagcccat gctggatcac ttcaagatcg cgcagcttcg gttgatgacg
     3661 aacaatccca ggaaggtaag ggcactggag caattgggtg tgcgaatcac cgagcgtatt
     3721 tcgttgcagg tgaacaggaa cccttataac gaaaaatacc tgagcaccaa ggcagcgaaa
     3781 cttgggcatt ggctgaaaga gcaccttgct gaaaaagagt gattgctcaa gggggtgatt
     3841 atggtgcctt tatatactcc gaaatccgaa tccgaggcgg cggtcattgc gtcgctgctg
     3901 catgcctatg gcgtctcatt ctttatgcgc ggcgcggcat tcagcacgat gtatcccggc
     3961 tccatgacca gcagcctcaa cgagcagaca ctgatggtgc gcgacgacca cgtcgaactg
     4021 gccaaaggct tgctgcattc atttttgacg ggtcaggaag cgatttgatt gcgatcgcca
     4081 gaaccttata tccagacgct tgtcagggtt aagcggcgct tgttttggct gtccgcgtgt
     4141 tattatcaag cgcgaagctg cttcataaat aaatcgtgct ttgggcgcaa acatggttgt
     4201 tgcgccacgg tgttttttag taaggtcgaa ccaattcatg tctaacgacg ccaccctcgc
     4261 gcaacccacc gtaaagtccc gacttccgtc cggcgaggcc gtcgtttccc gccccaggcc
     4321 cggcagcaaa tttgtcagcc cgcagggcac ccgggcagtc aaggatggta ttcgtccaaa
     4381 tgacaaaagc gatgttcccg acattggccc caagccggca tggctgcgcg ttcgcctgcc
     4441 ggcgggcgcg gcctacgaaa acgtgcgcaa catcgttaaa acccacaagc tgaataccgt
     4501 ttgcgccgaa tccaagtgcc ccaatattgc tgaatgctgg ggtcgcggca ctgcaacact
     4561 tatgctgatg gggtcggtat gcacgcgcgc ctgcaaattt tgttcagtca gcaccggcaa
     4621 tccgcatggc tggctcgaca aaaaagagcc cgagaatgtc gccgacgcgg tggccctgat
     4681 gaatctcaga tacgtggtgc tcacctcggt ggatcgcgat gatttgttcg atggcggcgc
     4741 gtctcattat gcggcatgcg tgcgcgccat tcacgcgcgt tcgccgcaga ccgcggttga
     4801 agcgctcacc cccgatttcg acggcaacgt gaagtccgtg gcgaaggtgc ttgatgcggg
     4861 cttggttact ttcgctcaga atattgaaac ggtcgaacga ttgacacacg ttgtgcgcga
     4921 tgttcgcgcc ggctatcgga aaacgttgaa cgtgcttgaa ttcgcaaaag ggtacgcgcc
     4981 taaaacgcac accaaaacca gcatgatgct tggtttgggt gaaaccgacg acgaaatcga
     5041 tcgcacactg gacgatattc gcgcggtagg cgttgacgtg gtgacgctgg gtcagtacat
     5101 gcgccccacg caaaaccact tgcccgttca acgctttgtg acacctgaac aattcaaggc
     5161 ctatcgcgaa ttggcgctgt ccaagggctt tcttgaagtg gtctcgggcc cgctggtgcg
     5221 ttccagctat cgcgcagagc gcgtgctgaa tcacgataac gttggtctgt gatcaggcgc
     5281 tatctgactg atagtcaata ggtcgatgcg cagtgaccgc cggtaggggc ctggcctgat
     5341 ccggcggcga ttagcggagg ctc
//
LOCUS       DATCXU010000066         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_929461,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000066 DATCXU010000000
VERSION     DATCXU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929461"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VIR04_01450"
     CDS             <1..451
                     /locus_tag="VIR04_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY9459445.1"
                     /translation="PYFMLRRAMRFGSLTALWFDMILLIPPAVLILHTRGGGVISRFS
                     EMPHLFWQIPVLGLISSVALVCYFTASRILPLGLFGIMGYIEPVLLFWVAFLLLNENM
                     SADAWWTYIPIWIAVMMIVLEGGYVWIRGDTARATAPRASKSKPPVG"
     gene            complement(486..1196)
                     /locus_tag="VIR04_01455"
     CDS             complement(486..1196)
                     /locus_tag="VIR04_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY9459446.1"
                     /translation="MTDFRQPIVPNRSNYVDAPSGVVRNRVLRNTYWLLTLSLIPTVL
                     GALAGLYTGVARVMAVSPGTSMIVFLVGAFGLMFLVERNKNNSMGVALLLAFTFFMGV
                     MLSQLLAHIMGLGNGTQLIVLAFGGTAAVFGAMAAVASTTKRDFTHWQKFLFIGVVVL
                     IVAMLANIFLQLPALMLTVSVLAIVIFSAFLLVDLQRVINGGETNYVSATLAIYLDLY
                     NVFVNLLMLLGVLGGNRN"
     gene            complement(1415..1861)
                     /locus_tag="VIR04_01460"
     CDS             complement(1415..1861)
                     /locus_tag="VIR04_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY9459447.1"
                     /translation="MSRIDFAFGVRDRLRTACEIIRKHYLAGREIVIYTQDAKLLERM
                     DLLLWGFDAAAFVPHVTVNDSLAARTPVTLTTQPPVARLPVQSSKQAAWLLNLDAQCP
                     PNVGQFERVLEIVSNSDEDRQSARERWRNYKADGHILHAHDFAKRA"
     gene            complement(1848..>2119)
                     /locus_tag="VIR04_01465"
     CDS             complement(1848..>2119)
                     /locus_tag="VIR04_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY9459448.1"
                     /translation="SRDANDGAWRLPLEDAYQEQLQSNFADTANVGGRPAGATTAACF
                     LSRFTKNYPWAHLDVAGTAWRSGKDKGATGRPVPLLVQYLINESH"
BASE COUNT          453 a          567 c          636 g          463 t
ORIGIN      
        1 tccctatttt atgttgcgcc gggcgatgcg attcggatcg ctgacagcgc tgtggttcga
       61 catgatcctt ttgattcccc ccgcagtatt gatcctgcat acgcgcggcg gtggagtaat
      121 aagccggttc agcgaaatgc cgcacttgtt ttggcagatt ccggtgctgg gtctgattag
      181 ttctgtggcg ctggtttgct atttcacggc cagccggatc ctgccgctgg ggctgtttgg
      241 gattatgggc tatatcgagc cagtgctgtt attttgggtt gcctttctgc tattgaatga
      301 aaacatgagc gctgacgcgt ggtggacgta cattccaatc tggattgcgg tgatgatgat
      361 tgttctggag ggagggtatg tgtggatcag gggcgatacg gcgagggcaa ccgcgccccg
      421 tgcatcaaag tccaaaccgc ctgtgggctg atgccgggtg gggcgcggcg cgcccacccg
      481 taaccttaat tgcgatttcc gccaagaacg cccagcaaca tgagcagatt gacaaacacg
      541 ttgtacaggt cgaggtaaat ggccagcgtg gctgaaacgt agttggtttc accgccattg
      601 atgacccgct gcagatccac cagcagaaat gccgagaaaa tgacgatggc cagaactgac
      661 accgtcaaca tgagcgccgg cagttgcaga aagatgttgg ccaacatggc aacgatcagt
      721 acaacgacgc cgatgaacag aaatttttgc caatgggtaa agtcgcgctt ggttgtgctg
      781 gcgacggcgg ccatggcgcc gaacacggca gcggtcccgc cgaaggccag gacaatcagt
      841 tgcgtgccgt tgcccagacc cataatgtgg gccagcaatt gcgacaacat gacacccatg
      901 aagaacgtga aggcaagcag cagcgccacg cccatcgaat tgttcttatt gcgttcgacc
      961 aggaacataa ggccgaatgc gccgaccagg aacacgatca tgctggtgcc ggggctgacc
     1021 gccatgactc gcgcgacgcc tgtatacaga ccggccagcg ctcccaacac ggtgggtatg
     1081 agcgacaaag taagcaacca gtatgtgttt cgtaacacac gattgcgcac aacgcccgac
     1141 ggggcgtcga cataattgct gcggttcgga acaataggtt ggcgaaaatc ggtcatgttg
     1201 gatcctttag tatcaactgc atttagcatg ctaccactat tcccgtccag aataacttac
     1261 aaataactga atgaacaagt taaacgtgca gtcaagttat caattgacgg gccggctaga
     1321 tttgccagcg gcttgattcc ctgttgttag aggcgcactg gcgcgattgg ttcccgccgg
     1381 gacggtgttt taccccttgc gggtgggtac acggctatgc gcgtttcgca aaatcgtgag
     1441 catgcagaat gtgcccatcg gccttgtaat tgcgccagcg ctcgcgagcg gattgccggt
     1501 cttcgtcgct atttgagaca atttccagca cacgctcgaa ttgcccgacg ttgggcgggc
     1561 actgggcgtc caggttaagg agccatgccg cctgcttgct gctttgcacc ggcaagcgtg
     1621 cgactggcgg ttgcgtggtt aacgttacgg gggtgcgagc cgccagcgag tcattcacgg
     1681 tcacatgtgg aacgaacgct gccgcatcga acccccatag caagagatcc atgcgttcga
     1741 gcagcttggc atcctgcgta tagatgacta tttcgcgtcc ggccagatag tgcttgcgga
     1801 tgatttcgca cgccgtacgc aggcggtctc gcacgccgaa ggcgaaatca atgcgactca
     1861 ttgatcagat attgcaccag caacggcacc ggccggcctg ttgcgccctt gtcctttcca
     1921 ctgcgccatg ccgtgccggc aacgtccaga tgcgcccagg ggtaattttt ggtgaaacgc
     1981 gagaggaagc acgcggctgt ggttgcgcct gcgggccggc cgccaacatt ggccgtgtct
     2041 gcaaaattgg actgaagttg ttcctggtag gcgtcttcga gcggcaagcg ccaggcgccg
     2101 tcgttagcgt cgcggctgg
//
LOCUS       DATCXU010000067        10669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_534982,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000067 DATCXU010000000
VERSION     DATCXU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534982"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VIR04_01470"
     CDS             <1..367
                     /locus_tag="VIR04_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY9459449.1"
                     /translation="FLNVHEGPQAISYRTKINPNMGMEPGMITSNEPGLYRPGRWGIR
                     IENLMVNRAAMTTEFGEFLKFETLTLCPIDTRCIERSLLTSAERTWLNDYHAEVRRRL
                     SALVQGPARQWLTDRTQAI"
     gene            443..2149
                     /locus_tag="VIR04_01475"
     CDS             443..2149
                     /locus_tag="VIR04_01475"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY9459450.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGLRVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISTRMRKVNNFTTGQIYESVLHKE
                     RRGDYLGKTVQVIPHITNEIQDFIARGAKLAWDGAADVAIVEIGGTVGDIESLPFLEA
                     VRQMSLRLGRNNSAFIHLTLVPYIATAGELKTKPTQHSVQKLREIGIYPNALLCRADR
                     PVPDEERAKISLFSNVPLDAVISVWDADSIYKIPSMLHKQGVDNLICDALAITPPAAD
                     LTMWDRLVDALDHPTGEVRVGMVGKYVDLTESYKSLTEALIHAGIHTHSKISIEYLDS
                     ERIETEGTGCLEQLDAILVPGGFGKRGIEGKIAAIRYARENGVPYLGICLGMQLAVVE
                     FARHVAGLGGANSTEFDPSAPHPVVALITEWRDREGKIEKRDQSSDLGGTMRKGAQRC
                     PVKPGTRAHAIYGDEVCERHRHRYEVNNVYVSRLEEAGMVISARTPTESLPEIMELPG
                     HPWFMGVQFHPEFTSSPRDGHPLFLSFIRAAIERRTQKKERSGAHDKRQGKQKDGALL
                     NS"
     gene            2157..3008
                     /gene="kdsA"
                     /locus_tag="VIR04_01480"
     CDS             2157..3008
                     /gene="kdsA"
                     /locus_tag="VIR04_01480"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY9459451.1"
                     /translation="MMKLCGFDVGLTRPFFLIAGPCVIESRQLALDTAGELKEITAKL
                     GIPFIYKSSFDKANRSSETSFRGLGIEQGLRILADVRDQINVPVLTDVHSTEHVADVA
                     AVVDVLQTPAFLCRQTDFIHACAATLKPVNIKKGQFLAPHDMVHVAAKARAAAIKAGG
                     DGNTIMVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGATSGGQR
                     EYVPVLARAAIAVGVAGVFMETHPDPEKALSDGPNAVPLSQMAALLESLLNIDHYVKG
                     AGLFDAP"
     gene            3072..3356
                     /locus_tag="VIR04_01485"
     CDS             3072..3356
                     /locus_tag="VIR04_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY9459452.1"
                     /translation="MTAYLIANVVVTNPEKYEDYKRLSTRAMSSSNAKVLARGGAVEA
                     LEGKAPGRTVILEFPSMTAARAFYDSWQYRRARNARERASITTMYIVEGL"
     gene            3445..5043
                     /locus_tag="VIR04_01490"
     CDS             3445..5043
                     /locus_tag="VIR04_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459453.1"
                     /translation="MARIAAPAPSPNGRVLSVRKSLMAMVGLCFVIMMVAIDQTVVGT
                     ALPTIVAELNGFELYAWVATSYLLTSVITIPVFGRLGDYFGRKHFVVASIIVFTLASI
                     LCGMSNTMLQLVLARALQGVGGGMLVGTAFACIPDLFPDSHVRLRWQVLMSAAFGIAN
                     AIGPSLGGFLTQQAGWRSVFYVNVPVGVLGLFFVWRFLPRIRQIKAGHIRLDWQGAIL
                     VALGLGSLQLFVEFLPAHGPALAMVLLAAFSLCSFVALVYWEKRCPEPLLPLDMFRNK
                     GLAALFFLSLLAGFAMFVILFYVPLLLQGGFGLTPQAAGLMITPLVVFITIGSILNGR
                     IITRIRNPNLMLYAGFVLLAVSCAGIVFMHRTTPSAVVMVNLMMGGLGLGFVLPNLTV
                     YTQEIAGRYLLGIATALLQSVRMIGGMLGMAIVGSLVTHYYVSSVRHAVRHIDSASSL
                     AQLEDPQILVNASVQAGFLARLQVLHLDGAALIELARISLVSAIHSGLLATLLVAIIG
                     FFLVRRVPPIVFSHAAAPLASVKE"
     gene            5077..5529
                     /locus_tag="VIR04_01495"
     CDS             5077..5529
                     /locus_tag="VIR04_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY9459454.1"
                     /translation="MQQIGRTYRALLAAFDANIGQPMPRWRVMLLLYQRGETSQKELA
                     CELRMDPAALTRQIKSIERSGWVRRHSDAQDNRLTNVILTPAGRDLVARTLPRRIAFI
                     EDALTDLSLDEMSALSAMLDVLEKRLRQQTPKAPGSKRLKKKVARRAP"
     gene            5677..6963
                     /gene="eno"
                     /locus_tag="VIR04_01500"
     CDS             5677..6963
                     /gene="eno"
                     /locus_tag="VIR04_01500"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY9459455.1"
                     /translation="MSAIVDIIGREILDSRGTPTVECDVLLESGAMGRAAVPSGASTG
                     TREAIELRDGDPSRYWGKGVLRAVENVNTEISEALMGLDAQEQTFVDRTLVDLDGTES
                     KERLGANAILAASMAVARAAADDSGLSLYRYFGGSGPMQMPVPMMNVINGGVHANNTL
                     DLQEFMILPLGAQSFREALRMGAEVFHALKGLIQQQGMSTAVGDEGGFAPNVANHEAA
                     IQLILRAIAEAGYEAGSQIALGLDCASSEFYRDGRYHLAGEGGVALTSRDFANLLGTW
                     CDKYPIISVEDGMAENDWEGWKLLSDQLGDKVQLVGDDLFVTNTKLLKRGIDEDIANS
                     ILIKVNQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAMQIKTGS
                     LSRSDRMAKYNQLLRIEEELADVATYPGRDAFYNLR"
     gene            7008..7343
                     /gene="ftsB"
                     /locus_tag="VIR04_01505"
     CDS             7008..7343
                     /gene="ftsB"
                     /locus_tag="VIR04_01505"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY9459456.1"
                     /translation="MRLLLIVLVLLTALIQYPLWWGKGGWARVHELQKKVAAQQETNE
                     ALIARNTAMQAEVQDLKSGTQAIEERARAELGMVKPDEVFVQILAPHEKEPGVKTPEL
                     ASPKDEKGN"
     gene            complement(7365..8339)
                     /locus_tag="VIR04_01510"
     CDS             complement(7365..8339)
                     /locus_tag="VIR04_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY9459457.1"
                     /translation="MHRSISGSSLIEFSIAAIPILLVGLGGIEAAQWFYLKQAVSLAL
                     VQAGRAGITEHANPKVIEAAFKRALLPLYPPTAKLTSEQRMQAKFEQRSARTGLPPWQ
                     IEIIGPSTQAFQDFSDKQSSKGRQTGLAAINNSYQAEQDKRYRANGWADGLGPRSGLS
                     IYQANTLVLRVTYLHEPLLPGMKGLMRALSTGADSYASQGMARGGYLPITQKATLAMQ
                     SDPLDWPSLPGRKVIKAQAIPIAFVPYPAEACRGIWCLESRRMPSTPPAIKADSVNSP
                     SGASPASAGAAAIQDPSAAPPPAPIPRPELSVAPDDPACGITLCCVAA"
     gene            complement(8426..8947)
                     /locus_tag="VIR04_01515"
     CDS             complement(8426..8947)
                     /locus_tag="VIR04_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY9459458.1"
                     /translation="MAIYQFETLVPTIDEDTFIFDSADIIGDVTIQAQASIWAHVSIR
                     GDNAPIKIGRGSNVQEGSVLHVDRDCPLTVHDHVTVGHQAMLHGCTIGEGSLIGMQAI
                     VLNNAKVGRNCLIGAGALVPEGRQIPDNSLVVGVGKIVRELSAEEIRAIHEGTEHYVN
                     RARLYRKSLKKLR"
     gene            complement(8928..10367)
                     /locus_tag="VIR04_01520"
     CDS             complement(8928..10367)
                     /locus_tag="VIR04_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459459.1"
                     /translation="MNDQYPELYSSYQWLVPSTFNIAEACVHRWAENPHEGRRIALYC
                     EDEFGQRTVWTYTRLSEVSRQLANGLVKMGVAQGDRVALVMGRRPEMVAAYMAIFSVG
                     AIAVPLSAQLEIGFLGACLRDAQARVAVVDAVTGPSLLKAKAQFPSLTQIISLGFQHD
                     WVIPWRSLLARQPITFKVPQTRASSPALLLYRPPGNGAIKGVLLPHCALIGNLPGFVA
                     SQNWFPNKRDIFWSPADWARSGGLMSALLPTLYFGHGIVGVAGRFSVPRAFKIMERYQ
                     VTNTFLSPEALTTMMMEISSPREHYQLALRSIMTTGEPLDGAVFDWCRHNLAVTPNET
                     FGQTEMNCVAGNSHQKWPTKPGSMGRPYPGHRLAILDASGQPCPAGVVGNIALNRYDM
                     YGHADPVLFLGYWHNESATEAKFMGDWCLTGDLASSDHQGNFWYAGHDNIHGQQETRG
                     TPAYTSPLPGVTISGSSIPDSDTHGNLSI"
     gene            complement(10481..>10669)
                     /locus_tag="VIR04_01525"
     CDS             complement(10481..>10669)
                     /locus_tag="VIR04_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY9459460.1"
                     /translation="IPLLAALLVQAATRISGAASMDLLIVLTPFTLFIWSWFDPDGQF
                     LHDRVLGTRLVGVGKLPV"
BASE COUNT         2167 a         2853 c         3198 g         2451 t
ORIGIN      
        1 tttcctgaac gtgcatgagg gccctcaggc catctcgtat cgcaccaaga ttaatccgaa
       61 catgggcatg gagccgggca tgatcacctc gaacgagccc ggcttgtatc gcccgggacg
      121 ctggggaatt cggattgaaa atctgatggt taaccgcgcg gccatgacca ccgaattcgg
      181 cgagttcttg aagtttgaaa cactgacctt gtgcccaatt gatacgcgtt gcatcgaacg
      241 gagtctgctg acatcggccg agcgcacatg gctaaatgat taccatgcgg aagtccgccg
      301 ccgcttgtcg gcgctggtgc aggggccggc caggcagtgg ttgaccgatc gcacgcaggc
      361 gatctagtta aaacaaagcc gggcggtccc ggctataatc caaatttcct gctcccgctc
      421 ggcgaccccg agcgtcatta caatgaccaa atatgtattt gtcaccggtg gggtagtgtc
      481 ctcattgggt aaaggcattg ccgccgcttc tcttgccgct attctcgagt ctcgcggttt
      541 gcgcgttacg atgctcaaac tggatcccta cattaatgtc gatccgggca ccatgagtcc
      601 gtttcagcat ggcgaagttt ttgtgactga agacggcgcc gaaactgatc ttgatctggg
      661 tcactacgag cgttttattt caacgcgcat gcgcaaggtc aataacttca cgaccggcca
      721 aatttacgaa tccgtattgc acaaagagcg ccgtggcgat tatttgggca agactgttca
      781 ggtcattccg cacatcacta acgagattca ggattttata gcgcgtggcg ccaagctggc
      841 ctgggatggt gccgccgacg ttgcgattgt cgaaatagga ggcacggtcg gcgatatcga
      901 atcgttgccg ttcctggaag ctgtgcgcca aatgagcttg cgcctgggcc ggaataattc
      961 ggctttcatt catttgacat tggtgcccta cattgcgaca gcgggcgagc ttaaaaccaa
     1021 accaacccag cactcggtgc aaaagctgcg cgaaatcgga atttatccga acgcattgtt
     1081 gtgtcgcgcc gaccggcctg ttcccgacga agagcgcgcc aagatttcgt tgttttccaa
     1141 cgtgccgctc gatgccgtga tttcagtatg ggatgccgat tcgatctata aaattccgtc
     1201 catgctgcac aagcaaggag ttgacaacct gatttgcgac gccctggcga ttacgccgcc
     1261 cgctgccgat ctgaccatgt gggatcgttt ggttgatgcg ctcgatcatc ccaccggcga
     1321 ggtgcgtgtg ggcatggtgg gcaaatatgt cgacctgacc gaatcctata aatcgttgac
     1381 tgaagcgctg atccatgcgg gcatccatac gcattcgaaa atttccatcg aatatcttga
     1441 ttcggaacgt attgaaaccg aaggaactgg atgtcttgaa cagcttgatg ccattttggt
     1501 gcccggcggg ttcggcaaac gcggaattga aggaaaaatt gcggcgatcc gctacgctcg
     1561 tgaaaatgga gtcccttatt tggggatttg tctgggcatg cagcttgccg tggtcgagtt
     1621 cgcccgtcat gtggcgggtc tggggggcgc gaacagcacc gaatttgacc cgtcggctcc
     1681 gcaccctgtt gtcgcgctga tcaccgagtg gcgggatcgc gaggggaaga tcgaaaagag
     1741 ggatcagtcc tccgacttgg gcggcacgat gcgcaagggc gcgcagcgct gtcccgttaa
     1801 accgggcacg cgggcacacg ccatatacgg cgatgaggta tgcgagcgcc accgtcatcg
     1861 ttacgaggtc aacaatgttt atgtgtcgcg tcttgaagag gccggcatgg tgatcagcgc
     1921 ccgcacaccc actgaaagcc tgccggaaat catggaactg cccggtcatc cctggtttat
     1981 gggtgttcag tttcaccccg aattcacatc gagcccgcgt gatggccatc ctttgttttt
     2041 gagcttcatc cgggctgcga tcgaacggcg cacacagaaa aaagagcgca gtggcgcaca
     2101 cgacaagcga cagggcaagc agaaggacgg cgcgttgctg aactcttgag gtgtatctga
     2161 tgaaattatg cggtttcgac gttggattga cgcggccctt tttcctgatt gcaggtccct
     2221 gtgtcattga atcgcgccag ttggcacttg acacagcagg tgagcttaaa gaaataacag
     2281 cgaagttggg cattccattt atttataaaa gctcgtttga caaggcgaac cgcagttccg
     2341 agacttcttt tcgtggcctg gggatcgagc agggtctacg gatattggcc gatgtgcgtg
     2401 accagataaa cgtgccagtg cttacggatg ttcacagcac cgaacatgtc gccgacgtcg
     2461 ccgccgtggt cgatgtattg caaacgcccg cttttttgtg ccgtcagacc gattttatcc
     2521 acgcctgcgc cgccaccctg aagccggtca atatcaaaaa aggtcaattt ctagcgcctc
     2581 acgatatggt gcatgtggcc gccaaggcgc gcgccgcggc cattaaggcg ggcggcgacg
     2641 gcaatacgat tatggtctgc gagcgcggcg cctcatttgg ctacaacaat ttggtttccg
     2701 atatgcgttc tctggcgatc atgcgtgaaa caggttgccc ggtagttttc gacgcgacgc
     2761 actcggtgca attgcctggc ggccaaggcg cgacctcggg cgggcagcgg gaatacgtcc
     2821 cggtattggc gcgcgccgcg attgcggtgg gcgtggcagg cgtatttatg gaaacgcatc
     2881 cagatccgga gaaggcgcta tccgacggcc ccaacgcagt ccccctgagc caaatggcgg
     2941 cattgctgga gtcgctgctc aatatcgatc attatgttaa aggcgctggc ttgtttgatg
     3001 cgccctaggc gcgtacttcc gagcatggaa ttacgctgtt aacccggcaa cacttatttc
     3061 gcgaggaccc catgaccgct tacctgatcg ccaatgtcgt tgtgaccaac cctgaaaaat
     3121 acgaagacta caagcggttg tcgacacgcg caatgtcctc cagcaacgcc aaggtactgg
     3181 cgcgtggcgg ggcagtcgag gccctggaag gcaaggcgcc gggccgcacg gtgatcctgg
     3241 agttcccgtc catgactgcg gccagggcgt tttatgactc gtggcaatac cgccgcgcac
     3301 gcaatgcgcg cgagcgcgct tctattacga ccatgtatat tgtcgagggc ctgtaagcga
     3361 tccagcgcgt cccttgttct ggcgtgagta tcagcgctac aatgcttgat tgatcaaaga
     3421 ttgtattgtc aaggtattgg tgtcatggcg cgtattgcag cccccgcgcc gtcccctaac
     3481 ggtcgcgttt tgtcggtccg caaatccctc atggcaatgg tgggtttgtg cttcgtcatt
     3541 atgatggttg caattgacca gaccgttgtc ggtacggcgt tgcccacgat tgtggccgaa
     3601 ctgaacggtt tcgaactcta tgcgtgggtg gccacgtcct atttattgac atcggtcatt
     3661 accattcccg tgtttggacg actgggcgat tatttcggcc gcaagcattt cgtggtggcc
     3721 tcgatcattg tgtttacgct ggcgtcgata ttgtgcggca tgtccaatac catgctgcaa
     3781 ctggtgctgg cccgcgctct tcagggggtg ggcggcggca tgctggtagg gacggcgttt
     3841 gcctgtattc cggacctttt tcccgattcg catgtgcgct tgcgctggca agtgttgatg
     3901 agtgccgcgt ttggcatcgc caacgcaata gggccgtcat tgggcggatt tttaacgcag
     3961 caggcgggct ggcgttcggt tttttacgtc aacgtgcctg tcggcgtatt gggtttgttt
     4021 ttcgtatggc gttttcttcc ccgtatacgt cagattaagg ccggtcacat acgtctggac
     4081 tggcagggcg ccattttggt ggcgctgggt ctgggcagtt tgcaattgtt tgtggaattt
     4141 cttccggccc acggacccgc cctggcaatg gtcctgctgg cggctttcag cctgtgttcg
     4201 ttcgtggcgc tggtttattg ggaaaagcgc tgccccgagc ccttgctccc tctggacatg
     4261 tttcgcaaca agggtctggc ggctttgttt tttttgtcgc tgcttgccgg tttcgcgatg
     4321 ttcgtcattt tattttatgt tccgctgttg ctgcagggcg ggtttggcct gacgccgcag
     4381 gccgcgggct tgatgattac gccgctggtg gtgttcatta cgatcggaag catactcaac
     4441 gggcggatta ttacgcgtat ccgtaaccct aatctgatgt tgtatgcggg ctttgtgcta
     4501 ttggcagtgt cctgtgcggg tattgttttc atgcatcgga ccacgccgtc cgcagtcgtc
     4561 atggtcaatc tgatgatggg tggtctggga ttgggatttg tcctgcccaa tttgacggtg
     4621 tacacgcagg aaatcgccgg ccgctattta ttgggcattg ccaccgcgtt gttgcaatcg
     4681 gtgcgcatga tagggggcat gttgggcatg gccatcgtgg ggtcgctggt aactcattat
     4741 tacgtaagca gtgtgcgaca cgcagtgcgg cacattgatt cggcctcctc gcttgcgcaa
     4801 cttgaagacc cgcaaatttt ggttaacgcc tcggtgcagg ctggtttcct tgcgcgtctg
     4861 caggtattgc atctggatgg agccgcgctt atagaactgg ctcgtatttc cctggtttcc
     4921 gcaattcatt cgggcttgct ggcgacgctc ctggtggcga taattggatt tttcctggtt
     4981 cgacgcgtgc ctccgatcgt tttttcgcat gcggcggctc cgctggcttc ggttaaggaa
     5041 tagaccgtgc cgatagagtt tcctcagctc caggtgatgc aacaaattgg ccgcacatat
     5101 cgggcgctgc tcgccgcgtt cgacgccaat atcgggcagc ccatgccgcg ctggcgtgta
     5161 atgttgctgc tctatcagcg cggtgagaca tcccagaagg agctggcctg cgagttgcgc
     5221 atggatccgg ccgccttgac gcgccagatt aaatctatcg agcgttcggg atgggtccgg
     5281 cgtcatagcg acgcgcagga taatcggctg accaatgtca tattgacgcc ggcgggacgc
     5341 gatctggttg cgcggacctt gccgcggcgc attgcgttca tagaagacgc gctgaccgat
     5401 ttgtcgcttg atgagatgag cgcgctcagc gccatgctcg atgttctgga aaagcgcctg
     5461 cgtcaacaga ccccgaaagc tcccggttca aaacgcctga aaaaaaaagt cgcccggcga
     5521 gcgccgtgat cgctaatttt taaaattgtc tgaacccgcg aaccaatata gactctatct
     5581 tgcgttgcgc aggtacaatc aagcggttat aaatgcctgt tcgggctgtc ggccgggcaa
     5641 gcatgaaatt attaattcag gctggacaca cacattatga gtgcaatcgt agatatcatt
     5701 gggcgcgaaa ttctggattc gcgtggcacc ccgaccgtcg aatgcgacgt gttgctggaa
     5761 tcgggggcga tgggtcgagc ggctgtgccg tcgggtgcct cgacaggaac gcgcgaagcg
     5821 attgaattgc gcgacggcga tccatcccgt tattggggaa agggggtttt gcgggcggtg
     5881 gaaaacgtca atacggaaat ctccgaggcg ctaatgggcc tcgacgcgca agagcagacg
     5941 tttgtcgatc gcacgcttgt cgatctggac ggaactgaat ccaaggagcg tcttggagcc
     6001 aacgccatcc tggcggccag catggctgtg gccagggcgg ccgctgacga ttcgggtttg
     6061 tcactgtatc gatatttcgg cggcagcggc cccatgcaaa tgcctgtgcc catgatgaac
     6121 gtcattaatg gcggcgttca cgcaaacaat acgctcgatc tccaggaatt catgattttg
     6181 cctcttggcg cacaaagctt ccgcgaggcc ttgcgcatgg gggccgaggt gtttcacgcc
     6241 ttgaaaggtt tgattcagca acaagggatg tcgaccgccg tcggcgacga aggcggcttc
     6301 gcgcccaatg tggctaacca cgaagctgct atccagttga tattgcgcgc catcgccgag
     6361 gcaggctacg aagccggatc gcagattgcg ctgggcctgg attgtgcaag ctcagaattc
     6421 tatcgcgatg gacgatacca tttggccggc gagggcgggg tagctctgac atcgcgcgac
     6481 ttcgccaatt tactgggcac ctggtgcgat aaatatccca tcatttccgt tgaagacggg
     6541 atggcggaaa acgactggga gggctggaag cttctctcgg atcagttggg cgacaaagtc
     6601 caactggtgg gcgacgacct gtttgtcacc aacaccaaat tgctcaagcg cggtatcgac
     6661 gaagacatcg ccaattcaat tttgatcaaa gtcaatcaaa tcggcacctt gactgaaacg
     6721 tttgcggcga ttgaaatggc caagcgcgcc ggctacaccg ctgttatttc gcatcggtca
     6781 ggtgaaaccg aagattcaac cattgccgat attgccgtgg ccaccaacgc catgcaaatc
     6841 aagacgggtt ctctttcgcg ttccgaccgc atggcgaaat acaatcaatt gttgcgcatt
     6901 gaagaagaac tcgccgacgt tgcgacgtat ccggggcgcg acgcctttta taatttgcga
     6961 taaacaaggc ctgtatggcc ttattgccca gggactgcgt ttaaagaatg cgattgctgc
     7021 tgattgtcct ggttctgctg acggccttga ttcagtaccc cttatggtgg gggaaaggcg
     7081 gatgggcgcg ggttcatgaa ttacaaaaaa aagtcgcggc tcagcaggaa acaaacgaag
     7141 cgctgatcgc gcgcaacaca gccatgcagg ccgaagtgca agacttgaaa tcgggcacgc
     7201 aggcgattga agagcgggcg cgcgccgaac tgggcatggt caagcccgac gaggtctttg
     7261 tgcagattct ggcgccgcac gaaaaagagc caggcgtcaa aactccggag ctggccagcc
     7321 cgaaagacga aaaaggcaat tagctgccgt tcgcgccgcg ttattcaggc agcgacacaa
     7381 cacagtgtta tgccgcaggc ggggtcatcg ggcgcaacac ttaattccgg acgaggtatc
     7441 ggcgccggtg gtggtgcagc gctcggatct tgaatggcgg cggcgcccgc actggcaggc
     7501 gaagcgcctg aaggcgagtt cacgctatcc gccttgatgg ccggcggtgt cgaaggcatc
     7561 cgccgggatt caaggcacca aataccacgg caggcctcgg ccggatacgg cacgaatgca
     7621 atggggatag cctgggcttt gattaccttg cggcccggca gcgaaggcca gtcgagcggg
     7681 tcggattgca tggcaagcgt tgccttttgc gtgattggca agtaaccgcc gcgcgccatg
     7741 ccctgactcg cgtaggagtc cgcgccggtg ctaagcgcac gcatcagtcc tttcatgccc
     7801 ggcaacaacg gttcgtgcaa ataggtaacg cgtaaaacca aagtattggc ctggtagatc
     7861 gacaggcccg agcgtgggcc aaggccgtcg gcccagccat tggcgcggta gcgcttgtcc
     7921 tgttcggcct gatagctatt gttgatcgcg gcaagccccg tttgccgtcc cttggatgat
     7981 tgcttgtcgg agaagtcttg aaaggcttgc gtggacgggc cgataatttc aatttgccag
     8041 ggaggcaggc ctgtcctggc actacgctgc tcaaatttcg cctgcatgcg ttgttcggat
     8101 gtcagtttgg ccgtcggcgg atatagcggc aacagcgcac gtttgaaagc ggcttcgata
     8161 actttggggt tggcgtgttc ggttatgccc gcgcggcccg cctggacgag cgccaggcta
     8221 acggcttgct tgaggtaaaa ccactgcgca gcttcgatgc cgcccaagcc cacaagcaat
     8281 atgggaatcg cggcgatcga aaattcaatc aggctggagc cggaaatgga acggtgcatg
     8341 aaggcagtgt gccttcgtcc gggccgcgcg tcgagcatca ggcatgataa attcgtgtac
     8401 ggatcaatcc gcgattgcga atcggtcagc gcaacttttt caggcttttg cgatagaggc
     8461 gcgcccggtt cacatagtgc tcggtgcctt catgtatggc gcgaatttct tcggcgctaa
     8521 gttcgcgcac gatctttccc acgcccacaa ccagggaatt atcgggaatc tggcgtcctt
     8581 cggggaccag tgcgcccgcg ccgataagac agtttcgacc cacttttgcg ttattcagca
     8641 caatcgcctg catgccgatc agcgaccctt ctccgatggt gcatccgtgc agcatggcct
     8701 ggtgccccac ggtgacgtgg tcgtgaaccg tgagagggca gtcgcggtca acatgcagaa
     8761 cactgccttc ctggacgttg ctgccccgtc caattttgat gggggcgtta tcgccccgta
     8821 tgctgacgtg agcccatatg cttgcctgag cctgtatggt tacgtcgccg ataatatcgg
     8881 cgctatcgaa gataaaggtg tcctcgtcaa tggttggaac aagcgtttca aattgataga
     8941 ttgccatgag tgtccgagtc tggaattgaa ctgcctgata tagtaacgcc aggcaaaggg
     9001 ctggtgtacg cgggtgtgcc ccgggtttct tgctggccgt gaatgttgtc atggcctgca
     9061 taccagaaat tgccttggtg gtcggacgag gccaggtcgc cggtcaaaca ccagtccccc
     9121 atgaatttgg cttcggtggc ggattcgtta tgccaatatc ccaggaacaa gacgggatcg
     9181 gcatgcccgt acatgtcgta cctgtttagt gcgatgtttc ccacgactcc ggcggggcag
     9241 ggctggccgc tggcgtcgag tatggcgaga cggtgccccg ggtacggacg gcccatgctg
     9301 cccggctttg tcggccattt ttgatggctg tttcccgcca cgcaattcat ttcggtttgt
     9361 ccgaacgttt cgttgggagt taccgccagg ttgtgtctgc accagtcgaa tacagcccca
     9421 tccagcggct ctccggtggt catgatggag cgcagtgcga gttgatagtg ttcgcgcggc
     9481 gagctgattt ccatcatcat ggttgtcagt gcttctggcg aaagaaacgt gttggtcact
     9541 tgatagcgtt ccatgatttt gaacgctctg ggaacggaaa atcgccccgc gacgccaaca
     9601 atgccgtgac cgaaatacaa ggtgggcagc aatgcgctca taagcccgcc cgagcgcgcc
     9661 cagtcggccg ggctccagaa aatatcacgc ttgttgggaa accagttttg cgaggcgacg
     9721 aaccccggca ggttgccgat taacgcgcaa tgtggcagca agaccccttt gattgcgccg
     9781 ttgcctggcg gccgatagag cagcaaggcg gggctggatg ccctggtttg cggcactttg
     9841 aatgtgatgg gctgacgcgc cagcaagctg cgccacggaa tgacccagtc gtgctgaaag
     9901 ccaaggctga taatttgggt aagcgatgga aactgcgcct ttgccttgag caggcttggc
     9961 cctgtaacgg cgtcgacgac cgccacccgt gcctgcgcat cgcgcaggca cgcgcccaaa
    10021 aagccgattt ccaattgcgc tgacagcggt accgcgatcg cgccgacgct gaaaatggcc
    10081 atataggcgg caaccatttc gggccgcctc cccatgacca atgcgacgcg atccccctga
    10141 gccacgccca tttttaccag tccgttagcc aattgccggc ttacttccga cagacgcgtg
    10201 taggtccaga cagtgcgctg gccgaactcg tcctcgcagt aaagcgcaat acgccggcct
    10261 tcgtgggggt tctcggccca gcgatggacg caggcctcgg cgatattgaa cgtcgaaggg
    10321 acaagccact ggtacgagga gtagagttcc gggtattggt cgttcatttt ggtctgggat
    10381 tagcgtgctg cgcacgcggg cgcaatcttc cttccgaagc atgaacgact cgccgattct
    10441 gtgcaatccc ctgtgttcac aaaattggcc ttataagggt ttatacgggg agcttgccga
    10501 caccgaccaa gcgtgttcct aatactctgt cgtgcagaaa ttggccgtcg gggtcgaacc
    10561 aggaccatat gaacagcgtg aaaggggtaa ggacgataag gaggtccatt gatgcggccc
    10621 cgctgatacg tgttgcggct tgcaccagca gagccgcgag caaggggat
//
LOCUS       DATCXU010000068         6591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_28867,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000068 DATCXU010000000
VERSION     DATCXU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28867"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VIR04_01530"
     CDS             complement(<1..384)
                     /locus_tag="VIR04_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY9459461.1"
                     /translation="MSASPIEYLLSRRSVKYVQAPGPSDHELSQILQAGMSAPDHGRQ
                     RPWRFALIRGAAIERLANLAITATRAAGKPVSPEKEASTRRWLKEVPLLIAVACRLDY
                     SNPKIPEHERMLATGAAVVMMLNAAH"
     gene            complement(400..1128)
                     /locus_tag="VIR04_01535"
     CDS             complement(400..1128)
                     /locus_tag="VIR04_01535"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY9459462.1"
                     /translation="MIELGVNIDHVATLRQQRLTSYPDPVQAALRAEEAGADAITLHL
                     REDRRHIQDADVADIRSRLRTRMNLECAITSEMLVIACRTLPEDVCLVPEKRAELTTE
                     GGLDVLGQFDAVKRAVHMLHDAGIRVSLFIDPDPAQIDAAQRADSSVIELHTGAFANA
                     VDAGAAEAELQRIRAAVAQGAGLGLRVNAGHGLHYGNVQTIAAIPGIAELNIGHAIVA
                     RALFDGWEKAVRDMKALVITAAHG"
     gene            complement(1215..2666)
                     /locus_tag="VIR04_01540"
     CDS             complement(1215..2666)
                     /locus_tag="VIR04_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY9459463.1"
                     /translation="MSQRVFRLTFLSVAISLAVAGCSLAPKYERPVAPIPSQYPYQPD
                     RTQAVGPGVAASAELGWRQFFRDPQLVALIGIALDNNRDMRVAVQRVQEARAQYGIAD
                     SSRYPTIGVGGNAQIQHNPPDLRNPRGSSTDSISRYYQAGVGMTSFELDFFGRLKNLS
                     RAAYQQFLATEQAQRTVHINLIAQVAEAYFGLRAAEQQRALTSRTLAAREKSYSLVNS
                     TFKAGVASALDLNQAKTQLDSVRADLATVTRNEARARNALSLLLGESIPQNVPKAAVF
                     DQDQLLAAIPVGLPSDLLQRRPDILGAENALLAANANIGAARAAFFPNISMTGLLGFA
                     SPALGGLFSSGNYFWSFAPQIQAPIFSGGSLSGNLDLAKARKNIAVAQYEQAIQTAFR
                     EVADALAGEATYSHQLDALRSFEQSASETLRLAQLRYDSGVDSFLQVQTAEISLYTAQ
                     QSLLQTGLDSLTNRVELYKALGGGWLRDSVAAR"
     gene            complement(2663..5866)
                     /locus_tag="VIR04_01545"
     CDS             complement(2663..5866)
                     /locus_tag="VIR04_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9459464.1"
                     /translation="MSQFFIERPIFAWVVALAITLAGLLAIPNMPVSQYPEVAPPAIT
                     INAVYPGASAKDVASSVSSVIENELNGATGLLYYESVSDSYGGAQITATFEPGTNPDL
                     AQVDVQNRISNVTSKLPGAVTQQGLKVRKSNASFLMVVTLSSTDGSMNQTALADYITR
                     NIQNPISRVPGVGTFRLFAAPRAMRIWVDPDKLTGYKMSMAEVNAAVARQTTLISAGI
                     IGSPPNPGEQRVTAPIVVNGELSTVDGFSKIVLRSNVDGSSVRVGDVARVEIGADNYQ
                     FGARLNGKPTAAFAISLATNANALSTAKGVKDRMQELSKFFPGNISYAIPYDTTPYVQ
                     LSIEQVLHTLLEAMLLVFLVMFVFLQNVRYTLIPALVVPIAILGAFAVMLAVGFTINV
                     LTMFAMVLAIGILVDDAIVVVENVERIMAEEGLPPKEATSKAMPQISGAVVGMTLTLA
                     TVFLPLAFMSGSVGVIYRQFSIALAVSIIFSGFLALTFTPALCVTILKPVPKGHHEVK
                     KGFFGWFNRSFERTTNRYESWVGRSLKRGGRVMLVYLLLVLALGWLYTRLPTSFLPVE
                     DQGYVIANIELPAGSTANRTIDIIKKVEDYFQKQPQFQNIITVQGFSFNGNGLNSAIA
                     FVPLKDFAQRKGEQNSAQAVGDRATQNLLFGLPDSMVFAIVPPAISSLGNASGFDMRL
                     EDRGGLGHDALVAASQQLLQLAAKSPVLAQTRVTGLGGGPQLRLTIDRDKAAALGVDF
                     SEAATLLSTSLGSSFVAKFPNMGWIQNVWVQADQDHRMNIDDIMKLNARNSAGEMVPM
                     SSFLKTDWEQGPVQVVRYNSYESIRISGSSAPGYSSGQAMAEMEKLVAQLPQGYGYEW
                     TGLSHQEKQAGSQSVILMGLAMLVVFMVLSALYESWAIPLSVMLMVPLGMLGATALIS
                     ALGMENNVYFQVGMITVIGLAAKNAILIVEFAKDTYALGGSITDAATEAARLRFRPIL
                     MTSFAFILGVVPLAIATGAGHASQNAVGLGVLGGMLAATPLAMLFVPTFFVVVLNLFK
                     TKPKLTGAHAKAPLTTIHHPTAPNQSEPRPSTDGDQA"
     gene            complement(5908..>6591)
                     /locus_tag="VIR04_01550"
     CDS             complement(5908..>6591)
                     /locus_tag="VIR04_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY9459465.1"
                     /translation="ATLQSADIDLGYTRVTSPIDGRIGKLLVTEGALVSASAATQLAT
                     VQQLDRVYVDVTRSTTEISRLRRDFASGVLEKMDGDAARARVILDDGSLYGQEGRLLF
                     SGVTVDPTTGQVGLRAEFPNPDQILLPGMYVRVRLQQGVDEKALLVPLQAIQRTADGL
                     SSLMLVKNGKAQSQAIDVGPEVNGKSIISKGLSPGDVVIVEGFQKIRPGVPVQAVPWK
                     APAAQPAAG"
BASE COUNT         1517 a         1954 c         1781 g         1339 t
ORIGIN      
        1 gtgagcggcg ttcagcatca tgacaacggc cgctccggtg gccagcatcc gttcgtgctc
       61 cgggattttc ggattggaat aatccagccg gcacgccacc gcaatcagca aaggcacttc
      121 cttcagccag cgccgcgtcg acgcctcttt ctctggtgaa accggtttgc cagccgccct
      181 ggtcgcggta atcgccaaat tggccagacg ctcgatggcg gcgccgcgaa tcaatgcgaa
      241 gcgccacggc cgctgccggc cgtggtcggg cgccgacatg ccagcctgca aaatttgaga
      301 cagttcatgg tcgctcggtc ccggcgcttg aacatatttg accgaacggc gcgagagcaa
      361 gtactcgatg ggcgatgcag acaatgtcaa ttctccgtgt cagccatgcg cggcggtgat
      421 aaccagcgct ttcatatcac gcaccgcctt ttcccacccg tcgaataacg ctctggccac
      481 aatggcatga ccaatattga gttcggcaat gccgggtatg gcggcaatgg tttgtacgtt
      541 tccataatgc aagccgtgtc ctgcgttgac ccgcaagccc aaaccggccc cttgcgccac
      601 tgcggcgcga atgcgttgaa gctcggcttc ggctgcgccc gcatcgaccg cgttggcgaa
      661 cgcgccggtg tgaagttcaa tcacgctaga atcagctcgt tgcgccgcgt cgatttgcgc
      721 cggatcggga tcgatgaata gcgacactct tatgcctgca tcgtgcagca tgtgaaccgc
      781 ccgtttgacg gcgtcaaatt ggcccagcac gtcaagccct ccctctgtcg tcagttcggc
      841 gcgtttctca ggaacgaggc agacatcttc gggcaaggtg cggcaggcaa tgaccagcat
      901 ttccgaagtg atggcgcact cgagattcat ccgggtgcgc aagcgtgacc ggatatcggc
      961 aacatccgca tcctggatat ggcggcgatc ttcccgcaaa tgcaaagtaa tcgcatcggc
     1021 gcccgcttct tcagcgcgca acgccgcctg aacgggatcc ggatacgacg taagacgctg
     1081 ctggcgcaaa gtcgcaacgt gatcgatgtt cacgcccagt tcaatcattg tcatcacttc
     1141 tatctcgcca tcagagaaac aaacaggtca ggcatcatta tcgcctgttg cactgcgtct
     1201 gtcttggcgc gccatcagcg agccgcgacc gaatccctga gccacccgcc tccgagcgcc
     1261 ttgtacagct cgacgcgatt cgtcaacgaa tccagccctg tctgcaaaag ggattgctgc
     1321 gcggtgtaga gactgatttc ggcggtttgc acctgcagga aactatcgac tccgctgtca
     1381 tagcgcaact gagcaagccg aagggtttcg ctcgcggact gctcgaacga acgcaaagca
     1441 tcgagttgat gggaataagt cgcctctccg gcaagagcgt ccgccacttc ccggaacgcc
     1501 gtctgaatcg cctgttcata ttgagcaacg gcgatgtttt tacgcgcctt cgccagatcc
     1561 aggtttccac tcagactgcc gcctgaaaaa atgggagcct gaatctgcgg tgcaaaggac
     1621 cagaaatagt tcccggaact aaataagccg cccagcgccg ggctggcgaa tcccaacagg
     1681 ccggtcatgg aaatattggg aaaaaatgcg gctctggccg cgccaatatt ggcattggcg
     1741 gcaagcaacg cattttcagc tcccaaaata tcgggccggc gctgcagcaa atcagacggc
     1801 aaacccacag ggatggcggc aagcaactgg tcctgatcaa aaaccgcggc cttgggcacg
     1861 ttttgcggaa tggattcgcc cagcaataaa gagagcgcgt tgcgagcgcg cgcctcgttg
     1921 cgtgtcacag tcgcaaggtc cgcgcgcacc gaatcaagtt gggttttagc ctggttcaga
     1981 tccagcgccg aggccactcc cgctttaaaa gtcgaattca ccaaagagta agatttttca
     2041 cgtgccgcaa gggtccggct tgtgagcgcg cgctgctgtt ccgcggcccg caggccgaaa
     2101 taagcctcgg cgacctgcgc aatcaaatta atgtgcaccg tgcgttgcgc ctgttcagtc
     2161 gccaaaaact gctgatacgc cgctcgcgac agatttttaa gacggccaaa gaaatcaagc
     2221 tcgaacgacg tcatgccaac gcccgcctga tagtagcgtg aaatgctgtc cgtagatgaa
     2281 ccgcgcgggt tgcgaagatc gggcgggtta tgctggattt gcgcatttcc cccgacacct
     2341 attgtcggat aacggctgct gtccgcaatc ccgtactgcg cgcgcgcttc ctgaactcgc
     2401 tgcaccgcta cgcgcatgtc ccgattatta tcgagcgcaa tgccgatcaa tgcgactagt
     2461 tgcgggtccc ggaaaaactg ccgccagccg agttcggcgc ttgcggccac gcctggcccg
     2521 acggcctgag ttctatccgg ctgataggga tactgtgacg gaattggggc gactggccgc
     2581 tcgtacttgg gagccagcga acaccccgca acagccaaag atatcgccac ggataaaaac
     2641 gttaatcgaa aaacgcgctg gctcatgctt ggtccccatc cgtggaagga cgaggctccg
     2701 attggttagg ggcggttgga tgatgaatcg tggttaatgg cgccttggcg tgggcgcctg
     2761 tcaatttagg cttggtttta aacaagttta gaacaaccac gaagaaagtt ggcacaaaca
     2821 acatcgctaa aggcgttgcc gccagcatcc cccccagcac acccagcccc actgcgttct
     2881 ggctggcatg gccggcgcct gtggcgatcg ccaacggcac aacgcccagg ataaacgcga
     2941 aggatgtcat cagaatcggg cgaaaccgca agcgcgccgc ctcggtggcc gcatcggtaa
     3001 tgctgccgcc aagagcatat gtatctttgg cgaactccac aattaaaatg gcgtttttgg
     3061 ccgcaagacc gatcaccgtg atcattccaa cctgaaaata cacattattt tccatgccca
     3121 gcgcggaaat aagagcggtt gcgcccagca tccccaacgg aaccatcaac atgaccgaaa
     3181 gcgggatggc ccagctttcg taaagagccg aaagcaccat gaataccacc agcatcgcca
     3241 gacccatcaa aatgacggac tggctgccgg cctgcttctc ttggtgcgat aagcccgtcc
     3301 attcataacc gtagccttgc ggcaactggg ccaccagttt ttccatttct gccatcgcct
     3361 gccccgaaga ataacccggt gcggacgagc ccgaaatgcg tatcgattca taactgttgt
     3421 agcgcacgac ctgcaccggc ccctgttccc aatccgtctt tagaaatgac gacatgggca
     3481 ccatttcgcc ggcgctattg cgcgcgttca gcttcatgat gtcgtctatg ttcatgcggt
     3541 ggtcctggtc ggcttgcacc cacacgttct gaatccagcc catgttcgga aacttggcca
     3601 caaaggatga acccagagaa gtcgaaagaa gcgtggcggc ctcgctgaaa tccaccccca
     3661 gtgcggcagc cttatcgcga tcgatggtca gccgaagttg cggaccacct cccaatccgg
     3721 ttacccttgt ttgcgccagc accgggcttt tcgccgccaa ttgcagcaat tgttgtgaag
     3781 ccgccaccaa ggcgtcgtgc cctaaccctc cgcgatcctc caggcgcatg tcgaaacctg
     3841 acgcattacc cagcgacgaa atggccggcg gcacaatggc aaacaccatt gagtcgggca
     3901 agccgaacaa caagttttgg gtagcccggt cgccaaccgc ctgagccgag ttttgctccc
     3961 ccttgcgctg ggcgaagtct tttagcggca caaatgcaat ggctgaattc aacccatttc
     4021 cgttgaaact aaagccttgc accgtaatga tgttttgaaa ctgaggctgc ttttgaaaat
     4081 agtcctctac tttcttgata atatcgattg tccgatttgc cgttgagccg gcgggcaact
     4141 cgatgttggc gatcacgtaa ccctgatcct cgacgggtaa aaaggatgtg ggcaggcgcg
     4201 tatacaacca ccccagagcc agcaccaata aaaggtagac cagcatcaca cgcccgccgc
     4261 gtttcaagct gcgtcctacc caactttcat atctgtttgt agtacgttcg aacgagcggt
     4321 taaaccatcc aaagaaacct ttttttacct cgtgatgccc cttcggaaca ggcttgagaa
     4381 tggtgacgca aagcgccggg gtaaaggtca gtgcgaggaa ccccgaaaag atgatcgaca
     4441 cggccagcgc aatcgaaaac tggcggtaaa tcacgcccac agagccgctc atgaacgcca
     4501 aaggcaaaaa tactgtagcc agggtcaggg tcattccgac tactgcaccg ctaatttgag
     4561 gcatggcttt gctggtcgct tctttcgggg gcaatccttc ttccgccata atgcgttcaa
     4621 cattttccac cacaacaata gcgtcatcaa ccaaaatgcc aatcgccagc accatcgcga
     4681 acatcgtgag cacattgatc gtgaagccca ccgcaagcat gaccgcaaac gcccctaaaa
     4741 tggcaatggg aaccaccagc gccggaatca acgtatagcg cacgttttgc aggaaaacga
     4801 acatgaccag aaacaccagc aacatcgctt cgagcaaggt atgaaggacc tgctcaatgg
     4861 acaactgcac atacggcgtg gtgtcgtagg gaatggcgta tgaaatgttg ccgggaaaga
     4921 atttggacag ctcctgcatt ctgtctttga cgcctttggc cgtcgatagc gcattggcat
     4981 tggtggcgag cgaaatagca aaggcggcgg tgggtttgcc gtttaaacga gccccgaact
     5041 gataattgtc ggcgccgatt tcaacgcggg ctacgtcgcc cacgcgcaca ctggaaccgt
     5101 cgacgttcga gcgcagcaca attttcgaga atccgtcaac cgtagacagc tcgccattga
     5161 cgactatggg ggcggttacg cgttgctcgc cgggattggg cggcgaaccg ataatgcccg
     5221 ccgaaatcag ggtggtttgc cgggccacgg cggcatttac ctcggccatg ctcattttat
     5281 agcccgtcaa cttgtcggga tcaacccaaa ttcgcatggc gcgcggggcg gcgaacaagc
     5341 gaaaagtgcc cacgccagga acgcgcgata tcgggttttg tatgttgcgg gtaatgtagt
     5401 cggccagggc ggtttgattc atcgaaccgt cggtcgacga caacgtaacc accatcagga
     5461 aactggcatt gctctttctt actttcaggc cctgctgcgt cacagcgcct ggcagtttgg
     5521 acgtgacatt ggaaatccgg ttttgcacgt cgacctgagc cagatcagga ttcgtacctg
     5581 gttcaaaagt ggctgtgatt tgcgcgccgc catacgaatc actgacggac tcgtaataca
     5641 gcaagccagt agcgccattg agctcgtttt cgataacgct cgacaccgaa ctggccacgt
     5701 ctttcgcaga tgcgcccgga tacactgcat ttattgttat ggcgggcggg gccacttcgg
     5761 ggtactgcga caccggcata ttcggaatgg ccaacaggcc cgccaaagta atcgccaaag
     5821 caaccaccca ggcaaaaatc ggtctttcaa taaaaaactg cgacatgtat gttcactcta
     5881 taagcattag cggtgcaaac cgccgcacta gcccgccgcg ggctgagctg cgggcgcctt
     5941 ccatggcaca gcttgaactg gcacgcccgg ccggattttc tggaaacctt caacaatcac
     6001 gacgtcgccg ggacttaatc ctttggatat gatggatttg ccattcactt cgggcccgac
     6061 atcaatcgct tgcgactgag ccttgccgtt cttgaccagc atcaagctgc tcaggccatc
     6121 ggcggtccgc tgaatggctt gcagcggcac aagcagcgct ttttcgtcga cgccctgttg
     6181 caagcggacc cgcacataca tgccgggcaa caaaatttga tcagggttgg gaaattcggc
     6241 gcgaaggccg acctggccgg tggtcgggtc gaccgtgaca ccggaaaaca acaaccggcc
     6301 ttcctggcca tacaaagagc cgtcatcgag aatcacacgc gcccgggcag catcgccatc
     6361 cattttttcc agcacgccgc tggcgaagtc gcggcgcaat cgcgaaatct cggttgtgga
     6421 acgcgtcaca tcgacataaa cccggtcaag ctgttgcacc gttgccagtt gcgtagccgc
     6481 gctcgcactg accagagcgc cctctgtgac cagcaatttg ccaatgcggc cgtcgatcgg
     6541 cgaagtaacc ctggtataac ccaagtcaat atcggccgat tgcagcgttg c
//
LOCUS       DATCXU010000069         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_539262,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000069 DATCXU010000000
VERSION     DATCXU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539262"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VIR04_01555"
     CDS             complement(<1..680)
                     /locus_tag="VIR04_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459466.1"
                     /translation="MSELLNLDQISLSYDTSRGTRPVVENLSLRLERGQIGCLLGASG
                     CGKTTVLRAIAGFEPLCSGSICLGDVSLSSVNHHLAPEHRHVGMMFQDYALFPHLSVE
                     KNIAFGLRRENKSNRARRVKELLCMVNLEDLANRYPHELSGGQQQRVALARALAPKPE
                     LLLLDEPFSNLDVDTRERLAFEVRDILKKLGHAAIFVTHNQAEAFAIADRIGVMQQGN
                     IIQWDTPHG"
     gene            complement(682..2343)
                     /locus_tag="VIR04_01560"
     CDS             complement(682..2343)
                     /locus_tag="VIR04_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY9459467.1"
                     /translation="MVSITRPHLRHGWRIGALTIALCVAAPVLSLFWFAIHGTLEHWL
                     HLFSYVLPSALGNTVALLSGVGVLVACLGTGSAWLVTAYRFPSSRILTWALLLPLAVP
                     TYIVAFAYLDILHPIGPVQTLLRHLLGYDNPRQFRLPDLRTLPGAIFLLGFVLYPYVY
                     LSTRVMFSTQAASLLEAARVLGETGRSVFFRVALPMARPAIAVGVSLALLETLNDIGA
                     SEFLGVQTLTVSIYNTWVTRSDLAGAAQIALAMLAIAVTLILLERRGRRRQRYANTQR
                     MRPLQAKRLTGLAAALAFSLGWLPIIIGFIAPALYLLNETINHFSLRGGVSAQLLSSG
                     YNTVMIALVATFATLICGLTAAWAARAGHSKSSRRSSVLYARIATLGYAIPGTVLAIG
                     LLTPFALVDGLLSWAVGYFTGNTPGLILAGSSTALVCAYVIRFLAIPIGGIEAGLARI
                     PPSLEHAARLLGETSTGALRRVHLPLLRPAIAATALLVFVDTMKELPATLLLRPMNFD
                     TLATWLYAEAARGAYEEGVVAALAIVLAGLLPVIFLARNQFTPGH"
     gene            complement(2327..3313)
                     /locus_tag="VIR04_01565"
     CDS             complement(2327..3313)
                     /locus_tag="VIR04_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9459468.1"
                     /translation="MAALSSGPAWADVNVYTTREPKLVQPLFDEFTKDSGIKVNTVFV
                     KDGLIERVKAEGKHSPADLLMTVDIGNLLDLVEAGVTQAVDSPIMDNAIPANLRGADK
                     QWYALSLRDRVAYVANDLNVQAITYEAFADPKWKGKVCTRSGQHPYNTALIAAMIAHN
                     GIQAAEQWLRDVKANLGRKATGGDRDVARDILGGICDIGIANAYYVGRMKNALPGSDA
                     RKWGDAIKVVRPSFAHAKTPGTHVNISGASVAKYAANKDDAIVLLEYLVSDKAQSLYA
                     NANYEYPVKAGVTLDPVVASFGSLVVDSLNLTEIAKHRKQASELVDKVGFNN"
BASE COUNT          732 a          930 c         1011 g          644 t
ORIGIN      
        1 ccatgaggcg tatcccattg aatgatattg ccttgttgca tgactccaat ccggtccgcg
       61 atggcgaagg cctctgcctg attgtgcgtg acgaatattg cggcatggcc taattttttc
      121 aggatatcgc gcacttcaaa agcaaggcgt tcgcgcgtat cgacgtcaag gtttgaaaac
      181 ggctcgtcga gcagtaatag ttcgggtttg ggcgccagcg cacgcgccaa ggcgacgcgc
      241 tgctgttgtc cgcccgacag ctcatgcggg taacggttcg ccagatcttc gagattgacc
      301 atacagagca gctctttgac gcgccgggcg cgattgcttt tattttcgcg gcgcagtccg
      361 aaggcgatgt ttttttcgac actaaggtga ggaaacagcg cgtagtcctg gaacatcatg
      421 ccgacgtggc ggtgttccgg cgccaagtga tgattcactg acgacaggct gacgtcaccc
      481 aggcagatgg agccggaaca cagcggctcg aatccggcaa tggcgcgcaa cactgtcgtt
      541 ttaccgcagc ccgacgcgcc caggaggcat ccgatctggc cgcgttcgag gcgcaacgaa
      601 aggttttcca ctacgggccg cgtccctcgc gaggtgtcgt agcttagcga gatttgatcc
      661 agatttagca gttcagacat atcagtgtcc cggagtaaat tggttgcgtg ccagaaaaat
      721 gacgggcagc agcccggcca gcacaatggc cagagcggca accactcctt cttcgtaggc
      781 cccgcgggcc gcttcggcat aaagccacgt agccagggtg tcgaaattca ttggccgcaa
      841 caataatgtg gcgggcagtt ctttcatggt gtccacaaac accagcaatg ctgtcgcggc
      901 gattgcgggg cgcagcaaag gcagatgaac gcgccgcaac gcgcctgtcg atgtctcgcc
      961 cagcaggcgc gccgcgtgct cgagcgaggg cgggatacgg gccaggccag cttcgattcc
     1021 tccaattgga atggcgagaa agcggatcac gtaagcgcat accagcgcag tcgacgaacc
     1081 cgcaagaatg agccctggcg tgttaccggt gaagtaaccg acagcccatg acaacaaccc
     1141 atcgaccaga gcgaagggcg taagcaaccc tatggccaga accgtgcccg gtatggcata
     1201 acccagagtt gcgatgcgcg catacagcac acttgatcgg cgtgacgatt tcgaatggcc
     1261 cgcccgcgcg gcccatgccg ccgtcaaccc gcatatcagg gtcgcaaacg tggcaacaag
     1321 cgcgatcatg acggtgttgt agccgctcga taataattga gccgaaacgc cgccgcgaag
     1381 actaaagtgg ttgatcgttt cattcaataa ataaagggcc ggcgcgatga acccaatgat
     1441 gattggcagc catcccagtg aaaaggcgag ggcggctgcg aggcctgtca ggcgttttgc
     1501 ttgcagcggc cgcatgcgct gggtgttggc atagcgttgc cggcggcggc cgcgacgctc
     1561 cagcaaaatc agcgtcacgg cgatggccag cattgccagc gcaatttggg cggcgccggc
     1621 caagtcggaa cgggtgaccc atgtgttgta gatggatacc gtcagggttt gcacgcccag
     1681 aaattccgag gcgccgatgt cgttcagggt ttcaagcaga gccagactga cgccgaccgc
     1741 gatcgccggc cgggccatgg gcagtgccac tctgaaaaac acgctgcggc cggtttctcc
     1801 aagaactcgg gcggcttcca gcaggcttgc ggcttgggtc gaaaacatca cgcgggtgct
     1861 taaataaacg taaggataga gaacaaaacc aagtaaaaaa atcgcgcccg gcaatgtgcg
     1921 cagatcgggc aggcggaatt ggcgcgggtt gtcgtagccc agcagatggc gaagcagggt
     1981 ttgcaccggc ccgatcgggt gcaagatatc caggtaagca aaagccacga tgtacgtcgg
     2041 aaccgccaaa ggcagcaaaa gcgcccaagt caaaattcgg ctagaaggaa accggtaagc
     2101 ggtgaccagc catgcgctgc cggttcccag gcaggcgacg agcaccccta cgcccgatag
     2161 cagcgccacg gtgttgccca gggcagaggg caacacatag gaaaacaaat gcagccagtg
     2221 ctcaagcgtg ccatgaatgg cgaaccagaa caaactgaga actggcgcgg ccacacataa
     2281 tgcaatcgtc aacgcgccga tgcgccagcc atggcgcagg tgcggtctag ttattgaaac
     2341 caactttatc cactaattcg ctggcctgct ttcgatgttt ggcgatttcg gtcagattca
     2401 gggagtcaac gaccagggag ccgaaactgg ccaccaccgg atccagcgtt acgcccgcct
     2461 tgaccgggta ttcgtagttg gcattggcat acaggctttg ggccttgtcc gagaccagat
     2521 attccagcaa aacgatggcg tcgtccttat tggcggcata ctttgccacg gaagcgccgg
     2581 agatattgac gtgtgtgccc ggtgtcttgg catgggcgaa ggaaggccgg acaaccttga
     2641 tggcatcgcc ccattttctg gcgtcgctgc cgggcagggc gtttttcata cgtccgacat
     2701 agtacgcatt ggcgatgccg atatcgcaaa tgccgcccag aatatcgcgg gcgacatcgc
     2761 ggtcgccgcc cgtggccttg cgtccgaggt tggccttgac gtcgcgcagc cattgctcgg
     2821 ccgcctggat tccgttatgg gcaatcattg cggcgataag cgcggtgttg taggggtgct
     2881 gacctgagcg cgtgcacact ttccctttcc atttcggatc ggcgaacgct tcgtaagtaa
     2941 tggcctgaac attaagatcg ttggccacgt aagcgacccg gtcgcgcagc gaaagcgcat
     3001 accattgttt gtcggcgccg cgcaggttgg cggggatggc attgtccata atgggcgagt
     3061 cgaccgcttg cgttacgcct gcttcaacca ggtcgagcaa attgccgatg tccactgtca
     3121 ttaaaaggtc ggcgggcgag tgcttgcctt cggctttcac tcgctcgatc aggccgtctt
     3181 taacgaacac tgtgttcact ttgatgccac tgtcttttgt gaattcatca aacaggggtt
     3241 gaacaagctt gggttcgcgc gtggtgtata cgttgacgtc ggcccaggcg ggccccgagg
     3301 acagggcggc caggccc
//
LOCUS       DATCXU010000070         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_15738,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000070 DATCXU010000000
VERSION     DATCXU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15738"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VIR04_01570"
     CDS             <1..102
                     /locus_tag="VIR04_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459469.1"
                     /translation="QQGVEIARRTVAKYREAEGIESVSLRKARAKLR"
     gene            186..461
                     /locus_tag="VIR04_01575"
     CDS             186..461
                     /locus_tag="VIR04_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY9459470.1"
                     /translation="MFICICNAITERQLQSAVADGAGTLSDLQGQLGIGTCCGCCIET
                     ANHYLPGGRYAGQVTTEKESGTHAGIAANDAQAGSDNTMVTVAVRRA"
     gene            544..1014
                     /gene="bfr"
                     /locus_tag="VIR04_01580"
     CDS             544..1014
                     /gene="bfr"
                     /locus_tag="VIR04_01580"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY9459471.1"
                     /translation="MKGDKTVIQHLNKQLKNELTAINQYFLHARILRHWGLNKMGKHE
                     YDESLGEMKHADRLIERILMLDGLPNLQDLHKIMVGETVPEILSCDYKLESAAQETVK
                     EAMAYCESVRDFVSRDLFQDILADTEEHIDWLETNIELVDKVGLENYLQSQMGD"
     gene            complement(1022..1660)
                     /locus_tag="VIR04_01585"
     CDS             complement(1022..1660)
                     /locus_tag="VIR04_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459472.1"
                     /translation="MSWRFIIAILLIAGGASAWGGLRLGNWLVAHGPVAKEIPEQSRE
                     TAVPVLDANGRPYVAQPPQPLVDGRLAAPQKLAEVAWKIPDQSLNATMANAAIPVATT
                     PITMVEAQRLASGGQNLVGIADVGNLGLAAGGHSNGPIQPIEVPDTTVRQAATHTPAK
                     NQAWQARLRQDLQVCSAQGFFERPTCAWAARNKYCEANNAWGQTRDCPAKNF"
     gene            complement(1699..3303)
                     /locus_tag="VIR04_01590"
     CDS             complement(1699..3303)
                     /locus_tag="VIR04_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY9459473.1"
                     /translation="MSITQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIQIAGSVK
                     ARKASRYASSDWMEIEKQRGISVASSVMQIEYRDCVLNLLDTPGHQDFSEDTYRVLTA
                     VDAALMVIDAANGVEPQTIRLLQVCRARNTPIITFINKLDREVREPLDLLSEIESHLG
                     MDAVPFSWPVGMGRSFKGVFDIRDDHMRVFKAGEERHGHHDEVIKGLRNPVMEQRFGD
                     MYTKALDEIELINAAAPVFDQAAFLAGKQTPVFFGSAINNFGVQEILDALVDLAPKPG
                     PRTAMEREVRPEEPKFSGVVFKVQANMDPAHRDRVAFLRVSSGKFERGMRLKVSRTGK
                     EIRPNNVVSFLSQRRELLDEAYAGDIIGIPNHGILQLGDVLTEGEVLHFTGLPFFAPE
                     LFQAVEVKDPLRTKQLRIGLTQLGEEGAIQVFRPENGGSLLLGAIGQLQFEVVAHRLK
                     SEYNADARLMPSRYNMARWITSENPKVLRKFLDANAAHIAYDVVNAVAFLASSPAQLR
                     VAQELYPEVEFHAMREHGGRIFGERI"
     gene            complement(3369..>3877)
                     /locus_tag="VIR04_01595"
     CDS             complement(3369..>3877)
                     /locus_tag="VIR04_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY9459474.1"
                     /translation="LTAGLLAGILCQVLIVGVGLGALLATSELAFAIVKWLGVGYLIY
                     LGWKQFSSEVSPITLHAGDAAAFRIRNLILRGWLVNVTNPKGTIFLLAVVPQFLNLSQ
                     PLTGQYVAIAATLNLTDLVAMGCYTALSARILKLMRSPRRIRWLNRTFGALFILAGVV
                     LATFSRHE"
BASE COUNT          832 a         1058 c         1146 g          841 t
ORIGIN      
        1 caacagggcg tggaaattgc ccggcgcacc gtcgcgaaat atcgcgaggc cgagggcatt
       61 gaaagcgtct ctttgcgcaa ggcgcgcgcg aagttaaggt aatccgcggg gccaggcgag
      121 cccgctgtgg gcggaaatgc aacgtggggt tgatttccgc cacccattag atgataattg
      181 ttctcatgtt tatttgcatc tgtaatgcga ttaccgaacg ccaattgcaa tcggctgtgg
      241 ccgatggcgc tggcacgctc tctgacctgc aaggccagct cgggattggc acgtgttgcg
      301 gctgttgtat tgagacggcc aatcactacc tgccgggcgg gcgctatgcg ggtcaggtca
      361 cgactgaaaa agaatccggc actcatgccg gcattgccgc caacgatgct caggctgggt
      421 ccgacaatac gatggtgacg gtagccgtcc ggcgcgctta gacagccgct ttaatcccct
      481 gattgtgaaa atgccctgat tgcatggtag gcttgttgtc gtgtcaacca ggagggttcg
      541 atcatgaaag gcgacaaaac cgttattcag cacctgaaca aacaacttaa aaatgaactg
      601 accgcgatca atcagtattt tttgcacgcg cgcattttgc gccactgggg cttgaacaag
      661 atgggcaagc atgaatacga cgaatcgctg ggcgaaatga aacacgccga tcgccttatt
      721 gaacgtattt tgatgcttga cggattgccc aatttgcaag acttgcacaa gattatggtg
      781 ggcgaaacgg tgccggaaat tctgtcgtgc gattacaagt tggagtcggc ggcccaggag
      841 acggtaaagg aagcgatggc gtattgcgag tcggtgcgcg atttcgtatc cagggattta
      901 tttcaggata ttcttgccga caccgaggag catatcgact ggctcgaaac caatatcgag
      961 ctcgtcgaca aggtgggtct ggagaactac ctgcaatcgc aaatgggcga ctgatagtct
     1021 gtcagaagtt tttcgcaggg caatcacggg tctggcccca cgcattgttc gcttcgcaat
     1081 atttattgcg cgcggcccag gcgcatgtgg gccgctcgaa aaagccctgc gcgctgcata
     1141 cttgcaaatc ctgacgcaag cgggcttgcc aggcctggtt tttcgcgggt gtgtgcgtgg
     1201 cggcttgcct gacagtggtg tcggggacct caatgggttg aatcggtcca ttggaatgac
     1261 cgcccgctgc aagtcctaaa ttgcccacat cggcaatacc caccagattt tgcccccccg
     1321 aggcgagccg ttgagcctcg accatcgtga tcggagtcgt ggcaacgggg atggcggcgt
     1381 tggccatggt cgcattaagc gattgatcgg gaatcttcca agcgacttcg gcgagctttt
     1441 gtggcgcggc caaacgcccg tccaccaaag gctgcggcgg ttgcgccacg tacgggcgcc
     1501 cgtttgcatc cagcacgggt acggcggttt cgcgggactg ctcggggatt tctttcgcca
     1561 cgggtccgtg cgccaccagc cagttaccca agcgcaggcc tccccaggcc gaagcaccgc
     1621 ccgcgattaa cagtattgca ataataaaac gccaagacat tctgatcctc ggtgtgcaag
     1681 ctcgatggag tccgaagctt aaattctttc gccaaagatt ctaccgccat gttcgcgcat
     1741 tgcatggaat tcgacctctg gatagagctc ttgagccact cgcaactgag cgggtgaact
     1801 cgccaaaaag gcgacggcat taaccacatc ataggcgata tgcgccgcgt tcgcatccag
     1861 aaacttgcgc aaaacttttg ggttttcaga tgtaatccat cgtgccatgt tgtaacgcga
     1921 tggcatcagt cgagcgtcgg cgttgtattc gcttttaagc cggtgggcca ccacttcgaa
     1981 ttgcagttgg ccgatcgcgc caagtaaaag tgacccgcca ttttcggggc gaaatacttg
     2041 aatcgcgcct tcttcgccaa gctgggtaag tccgatccgc agttgcttgg ttcgcaatgg
     2101 atctttgacc tcgaccgcct ggaataattc gggtgcgaaa aatggcaagc cggtgaagtg
     2161 aaggacctcg ccctcggtca gcacgtcgcc aagctgcaat atgccgtgat tggggatgcc
     2221 gataatgtcg cccgcatagg cctcgtccag cagctcgcgg cgttgggata aaaaggagac
     2281 cacattgttg gggcgtattt ctttgcctgt gcgggacact ttcagacgca tgccgcgttc
     2341 gaattttccg gaactgacgc gcaaaaacgc gacacggtcg cggtgcgccg ggtccatatt
     2401 ggcttgcact ttgaagacga cgccgctgaa cttgggttcc tcgggccgca cttcgcgctc
     2461 catcgctgta cgcgggcccg gcttgggcgc aaggtccacc aatgcgtcaa ggatttcttg
     2521 cacgccgaaa ttgttgatgg ccgaaccgaa gaagacgggg gtttgctttc cggccagaaa
     2581 cgcggcctgg tcgaaaacgg gcgcggcggc gttgatcagt tctatttcat cgagcgcctt
     2641 ggtgtacatg tccccgaagc gttgctccat gacggggttg cgcagaccct tgatcacttc
     2701 atcgtggtga ccgtggcgct cttctcccgc tttgaaaacg cgcatgtggt cgtcgcggat
     2761 gtcaaagacg cccttgaaag accggcccat gccgacgggc cacgaaaaag gtacggcatc
     2821 catccccagg tgggattcaa tttcggataa caggtccagc ggctctcgaa cctcgcgatc
     2881 caatttattg atgaacgtaa taatcggagt gttcctggcc cggcaaacct gcaatagccg
     2941 gatcgtttgc ggttcgactc cgttagcggc gtcgatcacc atcagcgcgg cgtccacggc
     3001 ggtgagcacg cggtacgtat cttcggagaa gtcctggtgg ccgggggtat cgagcaaatt
     3061 caggacacag tcccggtact cgatctgcat gaccgaggac gcgacagaaa tgccgcgctg
     3121 cttttcgatc tccatccagt ccgatgaagc gtagcggctg gccttgcgcg ctttgacact
     3181 gcccgcgatt tgaattgcgc cggcgaacag aagcagcttt tcagtcagcg tggtcttgcc
     3241 cgcgtcaggg tgggaaatga tggcaaaggt gcgccgtcgg gcgacttctt gggtaatgct
     3301 catagattaa attttagcgt gcgccttgcg gggcgggtcg aaggtctggt gcatttgcgt
     3361 tgtagccgtc attcatggcg gctgaatgtg gccaggacga cgccggccag aatgaatagc
     3421 gcgccgaatg tccggttaag ccagcgtatg cgccgcgggc tgcgcatgag cttcaaaatg
     3481 cgcgccgaca gcgctgtata acaacccatg gccaccagat cggtcaggtt cagcgttgcg
     3541 gcaatagcca catattgtcc tgtcaatggt tgcgacaggt tcagaaattg cgggacgacg
     3601 gccaacagga agatcgtgcc tttggggttg gttacattga ccagccaacc gcgcaggatc
     3661 aggtttcgaa ttctgaacgc cgctgcgtcg ccggcgtgca gggtaatcgg cgagacctcg
     3721 gaactgaatt gtttccaccc caggtagatc agataaccga cacccagcca ttttacgatg
     3781 gcgaaagcca gctctgacgt tgcaagcagc gcccccaaac ccacgcccac gatcaggacc
     3841 tggcacaaga ttccggccag caagcctgcg gtcagcc
//
LOCUS       DATCXU010000071         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_502253,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000071 DATCXU010000000
VERSION     DATCXU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502253"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VIR04_01600"
     CDS             <1..568
                     /locus_tag="VIR04_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459475.1"
                     /translation="AVAGASLGYAVMNLLMAATPLAMQMHEHPFSNIAIVLEWHVLGM
                     FVPSFFTGHLIKRFGVLPVMATGVALMLACVAIALSGVALMQFTLALLLLGAGWNFLY
                     IGGSTLLTEAYQPEEKNKTQGAMDFCVFTSVAISSFASGVLVTTQGWTWINLGSLAPL
                     ALVAAALWWMARHRAALRLAGAGAQETP"
     gene            565..2100
                     /gene="gntK"
                     /locus_tag="VIR04_01605"
     CDS             565..2100
                     /gene="gntK"
                     /locus_tag="VIR04_01605"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY9459476.1"
                     /translation="MNPPRRYVIGVDVGTTSTKAVVFEEDGRVVAKHHIDYPLLTPQP
                     SAAEQDPEQIYFAVLTSIKNVIEDGHVDAARIACVSFSSAMHSLIAVDKQDQPITRCI
                     TWADNRSAPWADRIKRELGGHAIYLRTGTPIHPMSPLSKLCWLRDTQPAVFARAARFI
                     SIKEYVFFRLFGCYIIDHSIASATGLFNLAALEWDPEALKLAGITADRLSRLVPTTHM
                     VHGLKSCAAQATGLHADTPFIIGASDGVLANLGANAIDTGVVAVTIGTSGAVRTVVDH
                     PHTDPQGRTFCYALTEKRWVIGGSVNNGGIVLRWLNDIFSPGDERATGQSDMDSYEAL
                     IQLAAQAPPGANGLIFHPYLTGERAPLWNASARGSFFGLALHHRKKDMVRAALEGVIY
                     NLYGVLCALESLAGTAQKIHATGGFARSELWRQMMSDIFNRNIDVPVSHESSCLGAAV
                     LGLYALGRINSLDAVSKMVGISAHYTPDARHVAVYAPLLAIYSSFPAKLQDEYECIAK
                     LQC"
     gene            complement(2110..>2496)
                     /locus_tag="VIR04_01610"
     CDS             complement(2110..>2496)
                     /locus_tag="VIR04_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HEY9459477.1"
                     /translation="PPLVDAASAAQELKSLLESDPPYNAKVSFKPDQGSATGWNAPVS
                     ATWLRTAMGAASQHYYGAPCGYIGQGGTIPLMGLLQKGFPKSQFMVCGVLGPQSNAHG
                     PNEFLHVPFAKKLTASVAHTIAALPA"
BASE COUNT          502 a          751 c          732 g          511 t
ORIGIN      
        1 ggcggtggcc ggcgcttcgc tcggctatgc ggtgatgaat ctgctgatgg cggcaacgcc
       61 gctggcgatg cagatgcatg aacacccgtt cagcaatatt gccatcgtgc ttgagtggca
      121 cgtgctgggc atgtttgtgc ccagtttttt caccgggcat ttaatcaagc gtttcggtgt
      181 attgccggtg atggcgacgg gcgtcgcgct gatgctggcg tgtgtggcga tcgcgctatc
      241 cggtgtggcg ctgatgcagt ttacgcttgc tcttctgcta ttgggcgcgg gctggaattt
      301 tctatatatt ggcggcagca cgctattaac agaggcgtac cagccggagg aaaaaaacaa
      361 gacccagggc gcaatggatt tttgcgtatt caccagtgtc gcaatcagct cgttcgcatc
      421 cggggtgctg gttacgacgc aaggctggac ctggatcaac cttggctcac tggcgcctct
      481 ggcattggtg gcggcagcgt tatggtggat ggcccggcat cgggcggcgc tgcgtctggc
      541 gggtgccgga gcacaagaga ctccatgaat ccgccgcgcc gctatgtcat cggggtggat
      601 gtcggaacaa ccagcaccaa agcggtggtt tttgaagaag acggccgggt ggtcgccaaa
      661 caccatatcg actacccttt gctgacgcca cagccgtcgg ccgccgaaca agatcccgaa
      721 cagatctatt tcgccgtgct tacgtcgata aaaaacgtaa tcgaagacgg ccacgtcgac
      781 gcggcgcgta tcgcgtgcgt ctcgttcagc tcggccatgc atagcctgat cgcggtcgac
      841 aaacaggacc agccgatcac ccggtgcatc acatgggcgg acaaccgcag tgctccgtgg
      901 gcggaccgga tcaagcgcga gctgggcggc cacgccatct atttgcgcac cggcaccccc
      961 atccacccca tgtcgccttt atccaaatta tgctggctgc gcgacaccca gccagcggta
     1021 tttgcacgtg ccgcgcgctt tatttcaata aaggagtatg tctttttccg cctcttcggc
     1081 tgctatatca tcgaccactc gatcgcttcg gcgacaggcc tgtttaatct ggccgcgctt
     1141 gaatgggatc cggaagcgct caaactggcg ggcatcaccg ccgaccggct atccaggctg
     1201 gtgccgacga cccacatggt gcacgggctg aagtcctgcg cggcgcaagc cacgggactg
     1261 catgcagaca cgccatttat aatcggcgcc agcgacggcg tgctggcaaa tctgggtgcc
     1321 aacgcgattg acacaggcgt ggtggcagta acgattggca ccagcggcgc cgtccgcacg
     1381 gtggttgacc atccacacac tgatccccag ggccgcacgt tttgctacgc cctgacagaa
     1441 aaacgctggg tcatcggcgg ctccgtcaac aatggcggca tcgtgttgcg ttggctaaac
     1501 gacatatttt cccccggcga cgaacgcgcg accgggcaat ccgacatgga ttcctacgaa
     1561 gcgctgatcc agcttgcggc ccaggcccca cccggtgcca acggcctgat tttccatcca
     1621 tacctgaccg gcgagcgcgc gccgctatgg aacgccagcg cacggggatc gtttttcggt
     1681 ctcgctttgc accatcgcaa aaaggatatg gtccgggctg cgctggaagg tgttatctac
     1741 aatctttacg gagtcttgtg cgcacttgaa agcctggccg gcacagctca aaaaattcac
     1801 gcgacaggcg gattcgcccg ctctgaacta tggcgtcaga tgatgtccga cattttcaat
     1861 cgcaacatcg acgtccccgt cagccatgaa agttcatgcc tgggcgccgc ggttctggga
     1921 ctctacgcgc tgggacggat caattcactt gacgccgtat cgaaaatggt cggcatcagc
     1981 gcccactaca cccctgacgc ccgccacgta gccgtatatg cgccgcttct ggcaatttat
     2041 tcaagctttc cggcgaaact gcaagacgaa tacgaatgca tcgccaagct gcaatgctaa
     2101 gcagccagcc tatgcgggca acgccgcaat cgtgtgcgcc accgaggccg tcagtttttt
     2161 cgcaaacgga acatgcagaa actcgtttgg accgtgcgcg ttcgactgcg gacccaacac
     2221 accgcacacc atgaattgcg actttgggaa tcctttttgc aacagcccca tcaaggggat
     2281 ggtgccaccc tgtccgatgt agccacacgg cgcgccgtag tagtgttggg aagccgcacc
     2341 catggccgtc cgcaaccagg tggccgatac gggcgcattc catccagtgg ccgagccctg
     2401 atccggcttg aatgaaactt tggcgttata gggcgggtcc gattccagca gcgatttaag
     2461 ctcttgcgcg gcggatgccg catcgaccag cggcgg
//
LOCUS       DATCXU010000072         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1143050,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000072 DATCXU010000000
VERSION     DATCXU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143050"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="petA"
                     /locus_tag="VIR04_01615"
     CDS             <1..514
                     /gene="petA"
                     /locus_tag="VIR04_01615"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY9459478.1"
                     /translation="PFVASFAPSAKARAAGAPVEAQVSNIPPGEMRTVEWRGKPVWLV
                     HRTPQQIKDLAKNDAELADPHSDRPGVTPAFAKNEYRSRKPELFVCVGICTHLGCSPS
                     PFFATGAQAGLPSSWEGGWLCPCHGSWFDLAGRVFKNKPAPDNLEVPPYVYSADGTHV
                     IIGLDEHGKA"
     gene            570..1958
                     /locus_tag="VIR04_01620"
     CDS             570..1958
                     /locus_tag="VIR04_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY9459479.1"
                     /translation="MAGEKTVDTTGLLGWIDKRFPLTSLYKAHLSEYYAPKNFNFWYF
                     FGSLALLVLVIQVVTGIFLVMSYKPDAALAFASVEFIMREVPLGWLIRYMHSTGASMF
                     FVVVYLHMLRGLFYGSYRKPRELIWIFGVAIFLSLMGEAFFGYLLPWGQMSYWGAQVI
                     VNLFSAIPFIGPELSLWIRGDYVVSDATLNRFFAFHVIAIPLVLIGLVVAHIMALHEV
                     GSNNPDGVEIKQGPKDRWGHPMDGIPFHPYYTVHDIMGVAIFLLVFAAIIFFGPEMGG
                     YFLEYNNFIPADSLKTPPHIAPVWYFTPFYSMLRATTSDFTWVMCAGAIIAALAILFK
                     SKLTGFWRVAIPVILIVIAVFTKTLDAKFWGVVAMGGSVVILFFLPWLDRCPVKSIRY
                     RPTWHKFLYALFIVNFIVLGFIGTQAPNATYNVISQVGTVIYLGFFFLMPVWSRMGTF
                     KPVPDRVTFRAH"
     gene            1977..2828
                     /locus_tag="VIR04_01625"
     CDS             1977..2828
                     /locus_tag="VIR04_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY9459480.1"
                     /translation="MIMIKKLLGALALSLTCAISFASEGGYPLDHAPNRINDLASLQN
                     GAKLFVNYCLSCHSASAMRYNKLTDIGLTEEQIKKNLLFTSDKVGNLMTIAMTPADGK
                     KWFGTTPPDLSVIARAKSSLSNSGVDYIYTYLRTFYRDASKHSGWDNLVFPNVAMPNV
                     LWELQGPGSLSHVTIHEAQQANGKTEWQRTVAQYDAQGFSNIKSEIVPNYHGISVDKP
                     TLTAADQNRSLAFDSNVADLSNFLGWMAEPAQLLRKQLGVWVILFLALFLVVAWRLNA
                     SFWKDVK"
     gene            2972..3580
                     /locus_tag="VIR04_01630"
     CDS             2972..3580
                     /locus_tag="VIR04_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY9459481.1"
                     /translation="MMVLYSGTTCPFSQRCRFVLFEKGMDFEIRDIDLYNKPEDIDVM
                     NPYGQVPILVERDLILYESNIINEYIDERFPHPQLMPADPVMRARTRLFLYNFEKELF
                     VHVSVLETRGDVNPKELDRARQNIRERLSQLAPMLLKNKYMLGDEFSMLDVAVAPLLW
                     RLDHYGIELPKNAAPIQKYAERIFSRPAYIEALTPSEKVMRR"
     gene            3586..>3767
                     /locus_tag="VIR04_01635"
     CDS             3586..>3767
                     /locus_tag="VIR04_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY9459482.1"
                     /translation="MPESSTKPYLIRALHEWCTDNGYTPHVVVTVDANTVVPPAHIHD
                     GQITLNIGTLATNQLIL"
BASE COUNT          802 a         1110 c          946 g          909 t
ORIGIN      
        1 tcccttcgtc gcctcttttg caccgtccgc aaaagcgcgt gcagcggggg cgccagtcga
       61 agctcaggtc tccaacattc caccgggtga aatgcgcact gtcgaatggc gcgggaaacc
      121 cgtgtggctg gtccatcgca caccacagca aatcaaggac ctggctaaaa acgacgctga
      181 actggccgac ccccattccg accggccggg cgtgacgccc gctttcgcca aaaacgaata
      241 ccgttcacgc aagcccgagc ttttcgtctg cgttggcatc tgtacccatt tgggttgctc
      301 gccatcgcca tttttcgcga ctggagccca ggccgggctg ccatccagtt gggaaggcgg
      361 ctggctgtgc ccttgtcacg gttcctggtt cgaccttgcc ggccgggtat tcaaaaacaa
      421 gcctgcgccc gataaccttg aagtaccgcc ctatgtctac tccgccgatg gcacacacgt
      481 cataatcggc cttgatgaac acggcaaggc ttagactaca cgacattgaa tccatcacac
      541 gcctcgtaag ataataaagg agccgtttca tggctggcga gaaaaccgtc gatacgacag
      601 gacttttggg ctggatagac aagcgctttc cgcttacatc cctctacaaa gcgcacctgt
      661 ccgaatatta cgcacccaag aatttcaatt tttggtactt ttttggctct ctggcccttt
      721 tggtgctggt catacaagta gtcaccggaa tcttcctggt catgagctac aagcccgatg
      781 cggcgcttgc gtttgcctcg gttgaattca tcatgcgaga agtgccgctg ggctggctga
      841 tccgctatat gcactccacc ggcgcgtcga tgttcttcgt ggtggtgtac ctgcatatgc
      901 tgcgcgggct gttttacggc tcgtaccgca agccgcgcga gctgatctgg atcttcggcg
      961 tcgccatttt tcttagcctg atgggcgaag cgtttttcgg ctacctgctg ccctggggcc
     1021 aaatgtcgta ctggggcgcc caggttatcg tcaatctgtt ctcggccata ccctttatcg
     1081 ggcctgaact ttcgctgtgg atacgcggcg attacgtcgt gtcggacgcc acgctgaacc
     1141 gcttcttcgc gtttcacgtt atcgccattc ctctggtgct tatcggcctg gtggtggctc
     1201 acataatggc gctgcacgaa gtgggatcaa acaaccccga tggcgtggaa atcaaacaag
     1261 gccccaaaga tcgttggggt cacccaatgg acggcattcc gttccatccg tattacaccg
     1321 tgcatgacat catgggcgtt gcgatcttcc tgctggtgtt tgccgccatc attttcttcg
     1381 gccctgaaat gggcggctac ttccttgaat acaacaactt catcccggcc gattcgctga
     1441 aaacgcctcc gcatatcgcc cccgtgtggt atttcacgcc gttttattcc atgctgcgag
     1501 ccaccaccag cgatttcacc tgggtcatgt gcgccggcgc catcatcgcg gcgctggcaa
     1561 tactgttcaa aagcaagctg actggcttct ggcgcgttgc catcccggtc atcctgatcg
     1621 ttatcgccgt tttcaccaaa acgctggacg cgaagttctg gggcgtggtc gccatgggcg
     1681 gctccgtcgt aatcctgttc ttcctgccct ggctggaccg gtgtccggta aagtccatac
     1741 gctaccgccc aacatggcac aagttcctgt atgccctttt catcgtgaat ttcattgtgc
     1801 tgggctttat cggcacacag gcgcccaatg cgacctataa cgtaatcagc caggtaggaa
     1861 ccgtcattta tctgggcttt ttcttcctga tgccggtatg gagccgcatg ggaacgttca
     1921 agcccgtccc cgaccgggtt acgtttcgag ctcattaggc ctataaactg tacggaatga
     1981 tcatgattaa aaagctgctt ggcgcccttg cgttatcact gacctgcgcg atttcctttg
     2041 cttctgaagg gggttatccg ctagatcatg cgccgaaccg aatcaacgac ctggcttcgt
     2101 tgcaaaacgg cgcgaagctg ttcgtcaatt actgtttaag ctgtcacagc gcaagcgcca
     2161 tgcgctataa caagctgacc gacatcgggc ttaccgaaga gcagatcaaa aagaatctgc
     2221 tgtttacttc cgacaaggtc ggcaatctca tgacgatcgc catgacgccc gccgatggca
     2281 aaaaatggtt tggaaccacc ccgcccgact tgtcggtcat tgcgcgcgcc aaatcaagcc
     2341 tgagcaattc gggcgttgac tatatctaca cctacttgcg caccttctac cgcgacgcat
     2401 ccaaacacag tggctgggac aacctggttt tcccgaatgt cgccatgccc aatgtgcttt
     2461 gggagcttca ggggccaggt tcgctatcgc atgttacgat acacgaggcg cagcaagcca
     2521 acggaaaaac cgaatggcaa cgcaccgtgg cgcagtacga tgctcagggg ttttccaata
     2581 tcaagagcga aattgttccg aactatcacg gcatatcggt cgacaagccc acgcttacgg
     2641 cggccgacca aaatcgctcg cttgccttcg acagcaatgt tgccgactta agcaacttcc
     2701 tgggatggat ggccgaaccc gcccaattgt tgcgcaagca actgggcgtt tgggtaattt
     2761 tgttcctggc cctcttcctg gtggttgcat ggcgcctgaa cgcctctttc tggaaagacg
     2821 ttaaataaaa cacaacctta cgccgagcgg cttcgcgcta cgcttaagcc gccggttacg
     2881 aaataggcgt agagttatac gcctgtttct ttttctggag ccgcactttt agtgcgctct
     2941 tgttttcatt tctacaattg gaacccgcga catgatggtg ctctattcag gaacgacctg
     3001 cccgttctct caacgttgcc gtttcgtgtt gttcgaaaaa ggcatggact tcgagatccg
     3061 cgatatcgat ctttacaaca agccggaaga tatcgacgtc atgaatccat acggtcaagt
     3121 gcccattctt gttgaacgcg acctgatttt gtacgaatcg aacatcatca acgaatacat
     3181 cgacgagcgc tttcctcatc cgcaacttat gccggccgat cccgtcatgc gcgcgcgcac
     3241 gcggttgttt ttgtataact ttgaaaaaga actctttgtc catgtgtccg tcctggaaac
     3301 tcgcggcgac gtcaacccca aagagcttga ccgcgcgcgc cagaacatcc gcgagcgtct
     3361 gtcccaactt gcgcccatgc tgctgaaaaa caaatacatg ctgggcgacg agttttcgat
     3421 gctggatgtt gccgtcgccc cgctgctttg gcgccttgac cattacggta tcgaactgcc
     3481 gaagaatgcc gcacccatac aaaaatacgc cgagcgcata ttttcgcgcc cagcttacat
     3541 tgaagcgtta acgccctctg aaaaagtcat gcgccgttga aaacaatgcc cgagtcttca
     3601 accaaaccct atcttatccg cgccttgcac gagtggtgta ccgataacgg ctacaccccc
     3661 catgtggtcg tgacggtaga tgccaacacg gtggtgcccc cggcacacat ccatgacggc
     3721 cagatcacgc tgaatattgg cacgctcgcg accaatcagt tgatcct
//
LOCUS       DATCXU010000073         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_559714,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000073 DATCXU010000000
VERSION     DATCXU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559714"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VIR04_01640"
     CDS             complement(<1..338)
                     /locus_tag="VIR04_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY9459483.1"
                     /translation="MKPALRIYRTLPRFDTHRPSAVTIGNFDGVHRGHQAILKRVSQL
                     AAQRDLSPTVMTFEPHPRTYFALRGKRPELIPTQISSLRDKLWFLACQDVQQLALERF
                     NQRLADMSAEA"
     gene            386..1078
                     /gene="purN"
                     /locus_tag="VIR04_01645"
     CDS             386..1078
                     /gene="purN"
                     /locus_tag="VIR04_01645"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY9459484.1"
                     /translation="MSAPISSSCRLVVLISGRGSNMQTIVHSAREQAVPANVCAVIAN
                     KADARGLQWAHEQGIETRLVSHRDYSSRGEFDQALVQVIDAYRPDYVLLAGFMRVLTP
                     EFVEHFNGRLINIHPSLLPAFPGLHTHQQALDTGVQWHGCTIHFVTSMLDSGPILAQG
                     AVPVYSDDTSDSLADRVLQIEHKLYARVVQWLAEGRVSLDSAQRVRVRGIATRSFVMA
                     PENSDLIISGAK"
     gene            1103..>2117
                     /locus_tag="VIR04_01650"
     CDS             1103..>2117
                     /locus_tag="VIR04_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY9459485.1"
                     /translation="MKSKDSYKSKDHRPYVMAAVRIEQVHSVLGEILQWEYPSDAVLS
                     RWMRANPKLGARDRGELADAIFDVLRHLRRYRQYSESGSGPATRRLAILGLASVFSAE
                     VLDTGLSESERDWLARVQSIDVNSLSRAVRSSLPDWLDERLAKLDDADALIQALNQPA
                     PLDVRVNPLKADRNDILAQMREGGALRFDPQPTPYSPWGIRLQGRPPVNRWPMFEKGE
                     IEVQDEGSQVLVTLVGPKRGEMIIDYCAGAGGKTLLLGALMRSTGRLYAFDVSAARLA
                     RAKPRFARSGLSNIVPVVIGANNDQRVKRLRGKAHRVLIDAPCSGLGTLRRNPDLKWR
                     QHPD"
BASE COUNT          397 a          579 c          664 g          477 t
ORIGIN      
        1 gcttccgccg acatatccgc cagcctttga ttgaagcgct ccagcgcgag ttgctgtaca
       61 tcctgacagg ccagaaacca cagcttgtcg cgcaagctgc ttatttgcgt gggaatgagt
      121 tccggccgtt tgcctcgcaa ggcgaaataa gtgcgaggat gcggctcgaa tgtcatgacg
      181 gtgggcgaca gatcgcgttg cgccgccagt tggcttactc gtttaaggat ggcctggtgg
      241 cctcggtgca cgccgtcgaa attgccgatt gtcaccgcgc tgggtcggtg ggtgtcaaag
      301 cgcggcaggg tgcgataaat tcttagagcg ggtttcacac acgagagttt acaattttgc
      361 attgtccggt tcaggaattt ttattttgtc tgctccaatt tcctcctctt gccgcctggt
      421 tgtgttgata tcgggccgcg gctccaacat gcaaaccatc gtccattccg cgcgtgaaca
      481 ggccgtgcca gccaatgtgt gcgcggttat cgccaataaa gccgacgcca gaggtcttca
      541 gtgggcgcac gagcagggta ttgaaacccg gctggtgtca catcgggact attcgtctcg
      601 cggtgaattc gatcaggcac tggtgcaggt tattgacgcc tatcggccgg attatgtttt
      661 acttgcgggc ttcatgcgcg ttttaacgcc cgagttcgtg gagcatttca atggccgtct
      721 gattaatatt catccttcgc tgctgcctgc tttccctggc ctgcacaccc atcagcaagc
      781 gctggataca ggggtgcagt ggcacggctg caccattcat tttgtgacgt ccatgctcga
      841 tagcggcccc atcctggcgc aaggggccgt acctgtgtac tcggacgata cttccgacag
      901 cctggccgac cgggtgttgc aaattgagca caagttgtac gcgcgggtgg tgcaatggct
      961 ggccgaaggg cgcgtttccc tggattctgc gcaacgggta cgcgtgcggg gcattgccac
     1021 tcgctcgttt gtcatggctc ccgaaaatag cgacttgatc atatccggcg caaaatagcg
     1081 cggggcccac caaggcaggt agatgaaaag taaagacagc tacaaaagca aagaccatcg
     1141 gccgtatgtc atggcggcgg tgcgcattga gcaggtccac agcgtactgg gcgagatttt
     1201 gcagtgggag tatccgtccg acgcggtgct ttcgcgatgg atgcgcgcca acccgaagtt
     1261 gggcgctcgc gatcgaggcg aactggctga cgccattttt gatgtgttgc gacacctgcg
     1321 ccgttaccgc caatattccg aaagtggttc gggtccggcg actcgccggc tggctatttt
     1381 gggcctggcg tcggttttta gcgccgaggt gctggacacc ggcttaagcg agtcggagcg
     1441 cgattggctg gcacgtgtgc aaagcatcga cgttaatagc ctttcgcggg cggtgcggtc
     1501 gagcttgccg gattggctcg atgagcggct ggcgaagctt gatgacgccg atgcgctgat
     1561 tcaggccctg aaccagccgg cgcctctgga cgtgcgcgtt aatccgctta aagctgatcg
     1621 caatgacata ttggcgcaga tgcgagaggg cggcgcgctg cgcttcgacc cgcaacctac
     1681 tccctattct ccttggggaa ttcgtttgca aggccggcct ccggtcaatc gctggccgat
     1741 gttcgagaaa ggcgagatcg aggtccagga cgaaggcagc caggttctcg tcacgctggt
     1801 ggggcctaaa cgcggagaaa tgatcattga ttattgcgcg ggcgcggggg gtaaaacctt
     1861 gttgctgggc gcactgatgc gttcgaccgg ccgtctgtat gcatttgatg tgtcggcggc
     1921 ccggctggcg cgcgcaaaac cgcgctttgc ccgcagcggc ttgtcgaata ttgtgccggt
     1981 cgtgatcggc gccaataacg accagcgggt taaacgcttg cgcggtaaag cgcatcgggt
     2041 gctgatcgac gctccgtgca gcggcctggg cacattgcgc cgcaaccccg atctaaaatg
     2101 gcgccagcat ccagact
//
LOCUS       DATCXU010000074         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_737910,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000074 DATCXU010000000
VERSION     DATCXU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737910"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VIR04_01655"
     CDS             <1..888
                     /locus_tag="VIR04_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459486.1"
                     /translation="NLPRAGLQQRSSPLLMKALDAAYGRTDDYFTWFEAPDLFSQRID
                     LVERLEHAQAVEFAAKRLIEQLCGWLQAKCVAVNTLFFNLHHEKGRYARPPSCIVLRF
                     SQPSWLPDDFARILTEQLRRTTLSAPVIAVELSIAGTTSRPAASQSLFPEPSQWLAKE
                     RHVLDLLRARLGFTRVLYAQPQADYRPECANRWGPLKTDLSSATGVPPQLPACSRPFW
                     LLPVPLTLATYDNHPVYNGAVLHLIQGPERVESGWWTESGHELRDYFIAQDGHDARYW
                     IYRQRESLQARWFLHGLFG"
     gene            898..>2929
                     /locus_tag="VIR04_01660"
     CDS             898..>2929
                     /locus_tag="VIR04_01660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY9459487.1"
                     /translation="MENSSLADYAELDCLSNFSFLRGASHPEELVQRAAELGYKALAL
                     TDECSLAGVVRAHTAAKQLSLKLIIGSQFNLRPAQASNAPMRIIALACNRNGYGNLSE
                     LITLARMRGKKGHYDLTLNDLLDPGPDLGHLQGLPDCLIIFKPEYGIDLQALCLCIES
                     LLPIFPGRLWLGLSLHHRYGDAQHQAILTQASKRYPIKLLATGQVEMHRRSRQPLHDT
                     LAAIRLHQTVRDCGYALESNAEHYLRSRLRLAQIYPADALAETRRLSDLCTFSLDEIK
                     YEYPTEIVPGGMTLQGYLRQETYAGAQKRYPQGISQKVRDQIEKELAIISELHYEPYF
                     LTVYDVVLFARQREILCQGRGSAANSAICYCLGITEVDPESGNSLFERFISVERNEPP
                     DIDVDFEHQRREEVIQYIYRKYGRQRAALTAVATTYRTRSVLRDTGKALGLDLDLISR
                     VTGSFRYWDGKQALLEKIAEQGLDADSHVARLWVGLAQTLMGFPRHLSQHPGGFVIAR
                     EKLCRLVPIENAAMPERSIVQWDKDDLDAMGFLKIDILALGMLSALKRTLLLVGQRRG
                     QPFRLQDIPKHDTPTFDMLCRADTVGVFQVESRAQMSMLPRLKPREFYDLVIQVAIIR
                     PGPIQGGMIHPYLRRRQGVEPVTYHNAQIKKILTRTLGIPIFQEQVMQIAMVA"
BASE COUNT          654 a          896 c          752 g          627 t
ORIGIN      
        1 aatctgcccc gggccggttt gcaacaacgc agcagcccct tgctgatgaa agctctggat
       61 gccgcctatg gccgcactga cgactacttc acctggtttg aagcgcccga tttattttca
      121 caacgcatcg atctggttga acgacttgaa catgcgcaag ccgttgaatt cgcggcaaaa
      181 cgccttatcg aacaactgtg cggctggctg caagccaaat gtgttgccgt caacactctt
      241 ttttttaacc tgcatcacga aaaaggacgc tatgcgcggc ctccctcatg catcgtcctg
      301 agattctctc agccatcgtg gctacccgat gattttgcac gcatattgac cgaacaattg
      361 cggcgaacca cgttaagcgc tcccgtcatc gccgtcgagc tttcaatcgc tggcacgacg
      421 tccaggcctg cggcaagcca aagcctgttt cccgagcctt cacagtggct ggccaaggaa
      481 cgccatgtgc ttgatttatt gcgcgcgcga ttgggtttca cgcgggtttt atatgcccag
      541 cctcaagccg actaccggcc tgaatgcgcc aacaggtggg ggccgcttaa aacagacttg
      601 tcatcagcca ccggcgttcc gccgcaactg cccgcctgtt cccgcccttt ctggcttttg
      661 ccagtccccc tcacgctggc cacctatgac aaccaccccg tttacaacgg cgccgtcctg
      721 catctgattc aaggcccaga gcgtgtggaa agcggctggt ggactgaatc tggccacgaa
      781 ttgcgcgatt acttcatagc ccaggacggg catgacgcac gctactggat ataccggcaa
      841 cgtgaatcgc tgcaagcccg ctggttttta cacggcttat tcggctaaag gctgcatatg
      901 gaaaattcca gcctggccga ctacgccgaa ttggactgcc tttccaattt ctctttttta
      961 aggggcgcat cgcatcccga ggaactggtg caacgcgccg ctgaacttgg ctacaaagca
     1021 ctggcactga ccgatgaatg ttcactggcg ggcgtggtgc gcgcccacac cgccgccaaa
     1081 cagctctcac taaaattgat tattggcagt cagttcaatc tacgtccggc ccaggccagc
     1141 aatgccccca tgcggatcat cgcgctggcc tgcaaccgca atggctacgg caatttatcg
     1201 gaactgatca cgctggctcg catgcgcggc aaaaaaggtc attatgactt gaccctgaat
     1261 gacctgctcg atccaggtcc ggatttgggt cacctgcaag gcttgcccga ctgccttatt
     1321 attttcaaac ctgaatacgg cattgatctt caggccctgt gtctgtgcat tgaatcgctg
     1381 ttgcccatat ttccaggacg cctgtggctc ggattaagct tgcatcaccg ttacggcgac
     1441 gcacagcatc aggctatcct gacgcaggca agcaaacggt atcccatcaa gctgctcgcc
     1501 accgggcagg ttgaaatgca ccgtcgatcg cgccagccac tgcacgacac cctggccgcc
     1561 atacgtctgc atcagacggt ccgcgattgc ggctatgcgc ttgaatccaa tgccgaacat
     1621 tacctgcgca gccgcctgcg cctggcgcaa atttatcccg ccgacgccct ggcggagaca
     1681 cgacgcttaa gcgatctatg cacattttcc ctggatgaaa tcaagtacga atacccaacc
     1741 gaaatcgtac ctgggggcat gacgctgcaa ggttacttgc gccaggaaac ctatgcgggg
     1801 gcccaaaagc gctacccgca aggcattagc caaaaagtca gggaccagat tgaaaaagaa
     1861 ctggcgatta tttcggaact gcattacgaa ccctacttcc tgacggttta cgacgtcgta
     1921 ctattcgctc ggcagcgcga aatcctgtgc cagggccggg ggtctgccgc caactccgcc
     1981 atctgttatt gcctgggcat taccgaagtc gaccccgaaa gcggcaactc tctgttcgaa
     2041 cgcttcatca gcgtcgaacg caacgaacct cccgacattg acgtcgactt cgagcaccag
     2101 cgacgcgaag aagtcatcca gtatatctac cgaaaatacg gccgccagcg cgccgcctta
     2161 acggctgtgg ccacgaccta tcgaacccgt agcgtactgc gcgacaccgg caaagcactg
     2221 gggcttgatc tggatcttat ctcgcgggta accggatcgt ttcgatattg ggacggcaaa
     2281 caggcattgc ttgaaaaaat agcggagcaa ggactggacg ccgattccca cgttgcccgt
     2341 ttgtgggtcg gcctggcgca aacgcttatg ggctttccgc gccatctgtc gcagcaccct
     2401 ggtggctttg tcatcgcgcg cgagaagctt tgccggcttg tgccgatcga aaacgcggcc
     2461 atgccggaac gcagcatcgt gcaatgggac aaggacgacc tcgacgccat ggggttttta
     2521 aaaatcgaca tactggctct gggtatgctc tctgccttaa aacgaaccct gctgctggtc
     2581 ggacagcgcc gcggtcaacc cttcagactg caagacatac ccaaacacga cacgcccact
     2641 ttcgacatgc tgtgccgcgc cgacaccgtg ggcgtttttc aagtcgaatc gcgtgctcaa
     2701 atgagcatgt tgccgcgcct gaagccacga gaattctacg acctggttat tcaggtggcc
     2761 atcattcgac caggccccat ccagggagga atgatccatc cttatctgcg ccgccgccag
     2821 ggggttgaac ccgtcacgta tcacaatgcg caaattaaaa agatattgac tcgcacactg
     2881 ggcatcccca tttttcagga gcaagtcatg caaatcgcca tggtggcgg
//
LOCUS       DATCXU010000075         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1129462,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000075 DATCXU010000000
VERSION     DATCXU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129462"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VIR04_01665"
     CDS             <1..690
                     /locus_tag="VIR04_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY9459488.1"
                     /translation="LAAAPWPDHPDVIAGIDCQARGTWLGITRQGRYAFLTNHRDPGR
                     NMAQARSRGALVSHFLTGHQSTAGYAQSVQDSKDAYNGFNLVVGDLSSACYVSNRTPQ
                     VVPQALRSGESHVLSNHLLDTPWPKAERLRLALHLFSLERLNDTLTPVFEILRDSTQA
                     EDTVLPSTGIPLARERLLSSPFVISPDYGTRCSTIVAVHASGRVLFSETSYDCAGNIT
                     ERHDWPFLIER"
     gene            755..1192
                     /locus_tag="VIR04_01670"
     CDS             755..1192
                     /locus_tag="VIR04_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY9459489.1"
                     /translation="MHAKTARSVMAQIASGAALGAVLLMGSPAQAQLPPAQQQGGIEY
                     VSGGIGLDESTAFKAAMAQFPLALTFASRDGDVAAYVADVRVVIRDNQNNDVLNVESN
                     GPYFLVKLPPGTYQVFATYNNKTLSQKVAIGASGTRQAKFEWQ"
     gene            complement(1229..1846)
                     /locus_tag="VIR04_01675"
     CDS             complement(1229..1846)
                     /locus_tag="VIR04_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY9459490.1"
                     /translation="MPIISKPDSSLSSLTFTPAQGKPEQLFVLLHGESASPDQLLPLA
                     RVIRQAFPQALLVLPYGPTRTLPALVARIRRIQVRHDLPGQQTALAGFSQGATMAFEA
                     SLEQPDLAGRVLAFSGSYKRPLTAAPLASTLHFLHGANDTQVSVEHVQSIYARLAELE
                     GDATLDVASGIGHELHDSLINRAIYRLQTCIPLRSWEAALSKTLH"
BASE COUNT          521 a          605 c          553 g          419 t
ORIGIN      
        1 ttggcggctg ctccctggcc cgatcaccca gatgtgattg ccggtattga ttgtcaggca
       61 cgcggcacat ggctgggcat aacacgacaa ggccgctacg cttttcttac caaccaccgc
      121 gatccaggcc gcaacatggc ccaggcgcgg tcgcgcggcg cactggtcag ccactttctg
      181 accggacacc agtcaactgc gggctacgcg caaagtgtgc aagattccaa agatgcctat
      241 aacggcttca acctggtggt cggcgacctg agtagcgcat gctatgtcag caaccggaca
      301 ccgcaagtcg tgccgcaggc cctgcgttct ggtgaatcgc atgtactatc gaatcatttg
      361 ctcgatacgc catggcccaa ggccgaacgc ttgcgccttg ccctgcatct gttctctctt
      421 gaacgattaa atgacacact gacgcccgtt ttcgaaatcc tcagagacag cacacaggca
      481 gaagatacag ttttgcccag cacaggcata ccgctcgcca gggaacgttt gcttagcagc
      541 ccgtttgtca ttagccccga ttacggcacc cgctgctcga ccatcgtggc cgttcatgcc
      601 agtggacgcg tgctattcag tgaaacaagc tatgactgcg ccgggaatat cactgaacgc
      661 cacgactggc cttttttgat cgaaagatag gctttacccg ctggatctgg aatgtataaa
      721 atgaaagccg ttgatttatc cccgggagaa ttcaatgcac gcgaaaactg caagatcagt
      781 catggcgcaa atcgcctcgg gtgccgcgct tggcgcagtg ctgctgatgg gctcgccggc
      841 ccaggcccag ttgccgccgg cgcaacaaca aggcggcatc gaatacgtta gcggcggcat
      901 aggtctggac gaatcaacag cattcaaagc cgctatggcg caatttcccc tggccctgac
      961 attcgcaagc cgggatggcg atgtggcggc ctacgtggcc gacgtgcgtg tggtcatacg
     1021 cgataaccag aacaacgacg ttttaaatgt cgagtcaaac ggcccttatt tcctggtcaa
     1081 attgccgcct ggcacatacc aggtttttgc gacctacaac aacaaaacac tgtcgcaaaa
     1141 agtcgccatt ggagcaagtg gaaccaggca ggccaagttc gaatggcagt aacgcgaagc
     1201 gattaaaaca gcaatgcccg gtgcaggctt aatgcagggt ttttgacaac gccgcctccc
     1261 aactgcgcaa aggaatgcag gtttgcaatc gatagatagc ccggttaatc aaagagtcgt
     1321 gcaactcatg accaatcccc gaagcaacat ccagcgtggc atcgccttcg agttcggcta
     1381 aacgagcata aatgctttgc acatgctcga cagaaacttg ggtgtcattt gcgccatgca
     1441 gaaaatgcag ggtcgaggca agtggcgcgg ccgtcaaagg acgcttgtag ctccctgaaa
     1501 atgccagaac acggcccgcc agatcgggct gttcaaggct ggcttcaaac gccatcgtgg
     1561 ccccctgaga aaacccggcc aaagcggttt gctggcctgg caaatcgtgc cggacctgaa
     1621 tccgccgtat ccgtgccacc agggcgggta acgttcgggt cggcccgtac ggtaaaacaa
     1681 gcagagcctg tggaaaggcc tgccggatta ctcgcgccaa aggaagcaat tgatcagggg
     1741 aggccgactc gccatgcaag agcacaaaaa gctgttctgg cttgccttgc gccggtgtaa
     1801 aggtaagcga agaaagcgac gaatcgggct ttgaaataat gggcatgggc aataaccaag
     1861 tctaaaaaaa ttgtacgcca taaattcaaa cctttataat gtactgtata ttaatacagt
     1921 ataaataaat gtggcaagcg ccttccaacc atgattcagt ccgctcccgc cctcgctttg
     1981 cgccaccccg aacgcatcca tcccgccctc tggcgcggat cccagcttgc gcaagcgcct
     2041 cacaacaccg tcccgaccgg atttgattgc ctgaaccagg aactacccaa tcaaggct
//
LOCUS       DATCXU010000076         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_229151,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000076 DATCXU010000000
VERSION     DATCXU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229151"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIR04_01680"
     CDS             complement(<1..219)
                     /locus_tag="VIR04_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY9459491.1"
                     /translation="MVNAAELLSGNHTSLVIVCDPDGLMAGVITKADVVRQISTCTGC
                     SCTTMVSDIMTREVIACHPDDQLIDVWAV"
     gene            complement(212..835)
                     /locus_tag="VIR04_01685"
     CDS             complement(212..835)
                     /locus_tag="VIR04_01685"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY9459492.1"
                     /translation="MTASWDPDVFEQRRAKIIKLEIDAGKTVADAAISAENKRWYKKD
                     GYFWVPKESRYTYLVDSSRQANVGNLRSKALAAIEGENVSFYDVLEHIDFTRKIGQSK
                     IPDIKLQQLITHFGLYCLRNEDFEFPDLLGAAYEYLIGEFADSAGKKGGEFYTPRSVV
                     RMMVRLIQPELKHDIYDPCCGSGGMLIAVKEYGDRVTGYCQCRRVGG"
     gene            943..2400
                     /locus_tag="VIR04_01690"
     CDS             943..2400
                     /locus_tag="VIR04_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459493.1"
                     /translation="MTTTTTDAAFQGNDRLLFGIIMGVIAFWLFAQTTLNIAPDMGRD
                     LGMSANTMNIAVAITALFSGIFIVVMGGLADRVGRMKIVRIGFYLNIIGSLLVGLTPV
                     GELATPVLLTGRALQGLSAACIMPASLAIVKAYWDGAERQRAISMWSIGSWGGSGLCS
                     LFGGLMSQNFGWRSIFFVSIAVCVLGLWMMRGTPESKAETKGDYKFDLPGVLTFMIAM
                     VTLQVVVTQGNNLGWTSPASLGLIAVTIIVGALFFKLEARSSNAFFDFSLFKNATYTG
                     ATISNFLLNAVAGTLIVSLQLVQLGGNMTAQNAGMLTLGYAIAIILFIRVGEKLLRSF
                     GARKPMIWGCLITGLAIIFLSPANLLLDDYKILAVFGYTLFGVGLAFYATPSTDAALA
                     NLPAAQSGAGSGIYKMASSLGAAFGVAISAAIFTALSANNKSIVWLEGVITFEGRQDN
                     LAVREAAIIALGFNVLMIAVAILSIMLTVPKGKKA"
     gene            complement(2405..>3075)
                     /locus_tag="VIR04_01695"
     CDS             complement(2405..>3075)
                     /locus_tag="VIR04_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY9459494.1"
                     /translation="SMKVRLFGRGSHGSQPQTSIDPVIMAASTTMRLQTIVSREIAPI
                     DSAVLTIGSLQAGTKENIIPDEATLKLNVRTYDENVRSTMLSAIKRICCAECAASNAP
                     REPEFTTLSGYPLTRNDVESTEKIARAFTAQFGDHARETKPAGASEDFSVFGRAWKVP
                     YVFWFVGGTDPQTYNKAKEEHKINTIPSNHSPKFAPVLDPTLKTGLQAMLTAASVWLC
                     PAQH"
BASE COUNT          632 a          853 c          849 g          741 t
ORIGIN      
        1 gaccgcccaa acatcaatta attggtcgtc aggatggcag gcaatgactt cacgcgtcat
       61 gatgtcagaa accatggttg tgcaactaca ccccgtgcag gtgctgattt gcctgaccac
      121 gtcggctttt gtaatgacac ccgccattag cccatcaggg tcgcatacga tcaccaggct
      181 ggtgtgattt ccgctcaaaa gttcggcggc attaaccacc aacgcgacgg cactgacagt
      241 aaccagtcac cctatcaccg tattccttga ctgcgatcag catgccgccg gagccacagc
      301 aagggtcgta gatatcgtgc ttaagctcgg gttggatgag ccgcaccatc atccgcacca
      361 ccgagcgagg cgtgtagaat tcaccaccct tcttgcccgc cgaatcggca aattcgccaa
      421 taagatattc gtaggctgcg ccgagcaggt cggggaattc gaaatcttca ttgcgtagac
      481 agtacagacc gaaatgcgta atgagctgct gcagcttgat gtcggggatc ttgctttggc
      541 caatttttcg agtaaagtcg atgtgctcca gaacatcgta aaagctgacg ttttcacctt
      601 caatcgcggc aagggccttg ctccgaaggt tgcctacatt tgcctggcgc gagctgtcga
      661 ccaggtaggt gtagcgcgac tctttgggca cccaaaaata gccgtccttc ttgtaccaac
      721 gcttgttctc ggccgaaatt gctgcatcgg caacagtttt gccagcatcg atttcaagct
      781 tgataatctt ggcacggcgc tgttcgaaga cgtcggggtc ccaactggcg gtaattaact
      841 attgatgggc gaagttataa ggatgatgta atctatcaaa agttgtcaat gttggttcag
      901 ttcgagttca gtccgccagg ctaacccacg aaggtcttac tcatgactac tacaaccact
      961 gacgcggcat tccagggcaa tgaccgtctg ctgtttggca tcatcatggg tgtgatcgcc
     1021 ttttggctat ttgctcagac tacattgaac attgccccag acatgggtcg cgatctcggc
     1081 atgtcggcca acacgatgaa tattgcggtg gcaattaccg cattgttctc gggcatcttc
     1141 atcgtcgtaa tgggtggcct ggccgaccgc gtcggccgca tgaagattgt gcgcattggc
     1201 ttctacctca atatcatcgg ttcattgctg gtcggcttga cgcctgtggg tgagttggcg
     1261 acgccggtcc ttcttaccgg gcgcgcattg caagggctgt cggccgcgtg catcatgccg
     1321 gccagcctgg caatcgtgaa agcctattgg gacggggccg agcgacaacg cgcaatcagc
     1381 atgtggtcga tcgggtcgtg gggcggctcg ggcctgtgct cgctattcgg tggcctgatg
     1441 tcccaaaact tcggctggcg ttcgatcttt ttcgtttcga tcgcagtctg cgtactgggc
     1501 ctgtggatga tgcgcggcac ccccgagagc aaggccgaaa cgaaaggcga ctacaaattc
     1561 gacctgcccg gagtactgac cttcatgatc gcgatggtga cgcttcaggt ggtggttacc
     1621 cagggaaaca atctgggttg gaccagccca gcgtcgctcg gcctgatcgc ggttacgatt
     1681 atcgtcggcg cactgttctt caagctcgaa gcaagatcgt ccaacgcctt tttcgacttc
     1741 agtctgttca agaacgcgac ctatacaggc gccacaatct ccaacttttt gctcaacgcg
     1801 gtagcgggca cactcatcgt atcgcttcag ttggtgcagt tgggcggcaa catgaccgca
     1861 cagaacgccg gcatgctgac cctcggctac gccattgcca tcattctctt catccgcgtt
     1921 ggggagaaac tgctgcgatc tttcggcgca cgcaagccga tgatctgggg ctgcctgatc
     1981 accggcctgg ccatcatttt tctttcaccc gccaaccttc tgctggatga ctacaagatt
     2041 ctggccgttt ttggctacac actgttcggc gtcgggctgg ccttttatgc cacgccctcc
     2101 accgacgcgg cgctggctaa cctgcctgcc gcccagtcag gtgcaggctc tggcatctac
     2161 aagatggcat cgtcgctcgg tgcagccttc ggtgtggcga tctcggccgc gatctttacc
     2221 gccctcagcg ccaataacaa aagcatagtg tggctggaag gcgtcatcac cttcgagggg
     2281 cgacaggaca atctggccgt gcgcgaggcg gccattatcg cccttggctt caatgtactg
     2341 atgattgcgg tcgccattct ttcgatcatg ctcaccgtgc cgaaaggcaa gaaggcctag
     2401 gtagctaatg ctgggccggg catagccaga ccgacgcagc agtcagcatg gcttgcaatc
     2461 cggttttcaa tgtgggatca agcaccggcg cgaatttggg tgaatgattg ctgggaatgg
     2521 tgttgatctt atgttcttct ttagccttgt tgtaagtttg aggatcggtg ccccccacga
     2581 accaaaaaac ataaggcact ttccatgcac gtccgaaaac gctgaaatct tcgctggccc
     2641 ccgcaggctt ggtttcccgc gcatgatccc cgaactgtgc cgtgaatgcg cgggcaattt
     2701 tctcggtaga ctcgacatcg ttacgggtca aggggtagcc gctaagagtc gtgaactcgg
     2761 gctcgcgcgg tgcgttcgaa gcagcgcact ccgcgcagca aatacgtttt atggctgata
     2821 acatcgtact gcgcacgttt tcgtcatagg tacgcacatt cagcttgagc gtggcttcgt
     2881 cggggatgat gttttccttg gtcccggcct gcagtgagcc aattgtcaat accgcgctgt
     2941 ctatgggagc gatttcgcgt gaaactattg tctgcagccg cattgtggtc gaagccgcca
     3001 taatgaccgg gtcgatagag gtttgtggct gcgaaccatg cgaaccccgg ccgaaaagcc
     3061 tgactttcat actgt
//
LOCUS       DATCXU010000077         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_726634,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000077 DATCXU010000000
VERSION     DATCXU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726634"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VIR04_01700"
     CDS             <1..1005
                     /locus_tag="VIR04_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEY9459495.1"
                     /translation="NLNAYEILVEQAETFSESTLLAVDGIEYDALLFSAPLVAWTRYQ
                     LPEGELNDAARQTMTRQLQTHILAEDAKVALVGQLVCFDQMPQTFQETWAWTRRLGLT
                     ALNAKSEPCAIKDAPDCEGMLADARFLVGAVVAPKGAPLFRWQTSAANAAQVREECFK
                     NWVREIGGTFGALFTGCNIEYLQPDAYYTNSREADRRIRPLALKAAVTWLQTAMHLSG
                     NDLRAVIAGCGETTVEEYRVGFSTRQSGDVIYGCIWPILSKEEALADSSDNGQADIPD
                     EIAALLKEFGVSDVKRLPGLYAIEFCDDCGAPYFPNALGEMIHPELPEETDLNPVHFH
                     "
     gene            1098..2138
                     /locus_tag="VIR04_01705"
     CDS             1098..2138
                     /locus_tag="VIR04_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEY9459496.1"
                     /translation="MAKQLACTAQCQKVRLWVDDLAAFRKIETAVDPSRPQQRLRNIE
                     LIHWTTPAPELTPCSVVIEAFGCNPPSEFIAKIGGQNSTWINLEYLSAEPWVEACHAL
                     PSIQSNGLRKFFFFPGFTTATGGLLREPGLIRKRDAWIRQPGLRWKQLEQIGVPDTSI
                     ELLRRGARQVFLFCYASAPAHALAHCLAQQSRPSVLIMPKGVYPSMGASQVQNVFIHE
                     IPFVDQDGFDQLLWGSDLNFVRGEDSMVRALWAGKPLIWQLYEQDNDAHLIKLDAWLE
                     RSGLAPAVCDLMRTWNTCKGPDFDAKLNTLLNSDAWPAWQSDATRWSDALAGLPDLTS
                     LLVAFCMEQRTG"
     gene            2219..2776
                     /gene="efp"
                     /locus_tag="VIR04_01710"
     CDS             2219..2776
                     /gene="efp"
                     /locus_tag="VIR04_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY9459497.1"
                     /translation="MKTAQELRVGNVVMIGHDAQVVLKAEYNKSGRNSAVVKLKFKNL
                     LTGSGGELVYKADEKFEIVVLENKECTYSYYADPMYVFMDEEYNQHEIEADSVGDALN
                     YLEEGMKVQVVFYEGRAISVDLPTIVVREITYTEPAVRGDTSGKVLKPAKINTGMDIN
                     VPLFCAIGDKIEIDTRTGEYRSRVM"
     gene            complement(2801..>3071)
                     /locus_tag="VIR04_01715"
     CDS             complement(2801..>3071)
                     /locus_tag="VIR04_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY9459498.1"
                     /translation="QGKATAVAAPASGQLAKLQADLMRHIRQIVRDTDDVGAQFAYEA
                     RRIHYGDAEERAIRGTATEQERESLAQEGIAVMPIPDFLDEDRLQ"
BASE COUNT          702 a          861 c          845 g          663 t
ORIGIN      
        1 aatcttaacg cctacgagat cttggtcgaa caggccgaaa ccttttcgga atcgaccctg
       61 cttgccgtgg acggaattga atacgacgcc ttgctgttct cggctccgct ggtggcatgg
      121 acgcgctacc aactgccgga gggcgaactg aatgacgccg cccgccagac catgacgcgc
      181 caattgcaga cgcatattct ggccgaagac gccaaggtgg ccctggtcgg acaactggtc
      241 tgctttgatc aaatgcccca gacttttcag gaaacctggg cctggacccg acgccttgga
      301 ctgacggcgc ttaacgccaa atccgagcca tgcgcgataa aagatgcgcc cgactgcgaa
      361 ggcatgcttg cagacgcacg gtttttggtt ggggctgtcg tggctccgaa aggcgccccg
      421 ctgttccgtt ggcagacaag cgcagccaat gcggcgcaag tgcgcgaaga atgttttaaa
      481 aactgggtgc gcgaaattgg cggcacattt ggcgctttgt tcaccggttg caatatcgaa
      541 tatctgcaac ccgacgccta ctacacgaac agtcgcgaag ccgaccgccg aatccgcccg
      601 ctcgcgctga aagcggccgt cacctggcta cagacagcga tgcacctttc gggaaacgat
      661 ttgcgcgcag tgatcgccgg gtgtggcgaa acaactgtcg aagaataccg tgtgggattc
      721 agcacccggc aaagcggcga cgtcatttac gggtgcattt ggccgatctt gagcaaagaa
      781 gaagcccttg cagattcaag cgacaacgga caagccgata taccggacga aatagccgcc
      841 ttgctgaagg aattcggcgt atccgatgtc aaacgcttgc cggggcttta tgccattgaa
      901 ttttgcgacg attgcggcgc cccctacttc cccaatgccc tgggcgagat gattcatccc
      961 gaactgcccg aagaaactga tctgaaccct gtccactttc attaatcatc gacacgaaca
     1021 cagcgccgac ttcgctcagc tttgatttat tttgccgggt tgtcgataac ttcggcgata
     1081 ttggcgtttg ttggcgactg gccaaacaac tggcctgcac ggcgcaatgc cagaaggtcc
     1141 gtttgtgggt cgacgatctg gccgcgtttc gcaaaataga aactgccgtc gacccttccc
     1201 gcccccaaca gcggcttcgc aacatcgagc ttattcactg gacaacgcca gcaccggaat
     1261 tgaccccctg ctccgtcgtc attgaagctt tcggctgtaa tccgccaagc gaatttatag
     1321 caaaaatcgg cggacaaaac agcacctgga tcaaccttga gtacctcagc gcggaaccat
     1381 gggtcgaagc ctgccacgcg ctgccgtcca tacaaagcaa cggcttgcgc aaattcttct
     1441 tttttccggg gttcaccaca gccaccggcg gactgctgcg cgaaccaggc ctgatacgca
     1501 aacgcgatgc atggatccgc caacccggtc tgcggtggaa gcagcttgaa caaattggcg
     1561 tacctgacac ctccatcgaa ttattgcgcc gcggtgcgcg gcaggtattt ttgttttgtt
     1621 acgccagcgc gcccgcacac gcgctggcgc attgcctggc gcaacaaagc cggccaagtg
     1681 tgctgatcat gcccaagggg gtttacccct caatgggcgc atctcaggtg caaaacgttt
     1741 ttattcacga aataccgttt gtcgatcagg acggcttcga tcaattgctt tggggcagcg
     1801 acttgaattt cgttcggggt gaagattcca tggttcgggc gctatgggcc ggcaagccac
     1861 tgatctggca actgtacgag caggacaacg acgctcattt gatcaagctt gacgcctggc
     1921 tggagcggtc cgggctcgca cccgcggttt gcgacctgat gcgaacatgg aacacgtgca
     1981 aagggcccga ttttgatgca aaactgaaca cactattaaa ttctgacgcc tggcccgcat
     2041 ggcaatccga cgcgacccgg tggtccgatg ccctggccgg cctgccggat ttgactagct
     2101 tgcttgttgc attttgcatg gaacaacgaa caggttaaaa tactgcgtct tgtacttctt
     2161 gctccggccc ggttcgtctg cgccaggtgg agcagctaat cccggagccc tttaaataat
     2221 gaaaaccgca caagaactgc gcgttggtaa tgttgtaatg ataggccacg atgcacaggt
     2281 cgtgctcaag gccgagtata acaagtcagg ccgcaattcg gctgttgtaa agcttaaatt
     2341 taaaaacctg ctgaccggtt cgggcggcga gctggtttat aaggccgatg aaaaatttga
     2401 aatagtcgtg ctcgagaaca aggagtgcac ttattcctac tatgccgatc cgatgtacgt
     2461 gtttatggac gaagaataca atcagcacga aatcgaggcc gacagtgtgg gtgatgcact
     2521 taattacctt gaagaaggca tgaaagtgca ggtcgtgttc tacgaaggcc gggccatctc
     2581 ggttgatctg ccgactattg ttgtgcgcga aatcacttat accgaacccg ctgtgcgggg
     2641 cgatacgtcg ggcaaggtgt taaaaccagc caaaataaac accggcatgg acattaacgt
     2701 gccgctgttc tgcgctatcg gcgacaagat cgaaatcgac acacgcacgg gcgaataccg
     2761 cagccgggtc atgtaaagag aaccgccgcc ctattgtttg ttattgcagg cggtcttcgt
     2821 ccaggaaatc aggaatcggc atgacggcaa tgccttcctg ggcaagggat tcacgttcct
     2881 gctcggtcgc cgtgccacgg atggcccgtt cctcggcatc accataatgt atccggcgtg
     2941 cctcgtaggc gaactgcgcg ccgacatcgt cggtgtcgcg cacgatttgt cgaatatgcc
     3001 gcataaggtc agcctgcaac ttcgccaatt gcccactggc aggcgctgcg acggccgttg
     3061 ctttgccttg c
//
LOCUS       DATCXU010000078         5048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_383459,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000078 DATCXU010000000
VERSION     DATCXU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383459"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..296
                     /locus_tag="VIR04_01720"
                     /pseudo
     CDS             28..296
                     /locus_tag="VIR04_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(357..782)
                     /locus_tag="VIR04_01725"
     CDS             complement(357..782)
                     /locus_tag="VIR04_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEY9459499.1"
                     /translation="MSNFKQVEENVFIGPQPTQHDLHDAKQRGIKSVIDFRMPAETTA
                     SNEALTKNAGLDYTNIPVNKGHLAVDQIGQLDAVMRSKNGPFLLHCATGARAALLYSL
                     NQASQQNWTVEQTFEAAKKMGFDLKATPEFSTFVKETID"
     gene            complement(818..1567)
                     /locus_tag="VIR04_01730"
     CDS             complement(818..1567)
                     /locus_tag="VIR04_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9459500.1"
                     /translation="MIFRQLFEPLSSTYTYLLGCQDTGQAVLIDPVVVSMDRDLFELN
                     LLGLTLVYTVDTHIHADHVTAALELKKKVGSKIAAPAYDRLPCTDLGIEEGEPFVLGS
                     IRLEPLHTPGHTDGHFAYLFGDRIFTGDALLIDGCGRTDFQNGNAGALYKSVHEKLFS
                     LPDETLVDPAHDYNNRHVSSIAQEKQHNSRLGQERTLEQFREIMANLNLPYPTFIDFA
                     VPANKRCGVCPDHLPENLGKYCARVVESRQG"
     gene            complement(1660..2091)
                     /locus_tag="VIR04_01735"
     CDS             complement(1660..2091)
                     /locus_tag="VIR04_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEY9459501.1"
                     /translation="MSISKLFFTALLALPLPVLAAGMSVTLYKNPDCGCCDRYAQYLR
                     SNGFEVQTINTDNLSKIKRAHAVPEKLEGCHTALIDGYVFEGLVPVENIQRVLKTHAP
                     IKGLSVPGMPVGAPGMAGNKTGTINVYYLDASPTPKVFSHF"
     gene            complement(2131..2628)
                     /locus_tag="VIR04_01740"
     CDS             complement(2131..2628)
                     /locus_tag="VIR04_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459502.1"
                     /translation="MKSKQIAAVFFTALIASAGVSALAAESPGQLDKSNTGAQGNMGA
                     GMMQGGAMGNGSDANRPDANGKHMGRSMMQGGMMGNDSRGCGMQGGMMGNGMMSGMMG
                     GGNGMMRGGAMDGGMMRGHAMMPQLPAGNEKLQLKMQAEIMQKVGEIVAKYADQVVTN
                     KDGAQ"
     gene            2825..2962
                     /locus_tag="VIR04_01745"
     CDS             2825..2962
                     /locus_tag="VIR04_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459503.1"
                     /translation="MAETSVVAATAGQNTHATHAGARVMYAAPTEGSGEETLDGATIA
                     L"
     gene            3026..3337
                     /locus_tag="VIR04_01750"
     CDS             3026..3337
                     /locus_tag="VIR04_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459504.1"
                     /translation="MLTSWQGAPFATVITTALTDIGGVLACVGGANEPSANIKKMMCA
                     MTIDIRCETFLMMNQFIPEPPAITSLIAALLAPNALALFDEHVEYVMYPSDSARWMTK
                     K"
     gene            3461..3793
                     /locus_tag="VIR04_01755"
     CDS             3461..3793
                     /locus_tag="VIR04_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEY9459505.1"
                     /translation="MSLRTTTLMVLFTTALAIPVSSFAQWIPDNSELCSHNVTNTTSM
                     KTHAQVLQELQQAKADPSWATRQGQETGSWPDKNAQPDKTREQVEHALNSVTPAQRAY
                     QQSFYAGA"
     gene            3916..4320
                     /locus_tag="VIR04_01760"
     CDS             3916..4320
                     /locus_tag="VIR04_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459506.1"
                     /translation="MKLSRGSRLTAALVALVSMLFMQMALAGYACPGYKTGLGHDKVA
                     MAQSASDMAMPGCEQVDLAQPNLCQAAIQIAHQSLDKPQVPHVHAFVPVTTMLVAEAS
                     ALYRPITGSLKVERPQRHTAPPISTLRSRLRI"
     gene            4395..>5048
                     /locus_tag="VIR04_01765"
     CDS             4395..>5048
                     /locus_tag="VIR04_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY9459507.1"
                     /translation="MSLPFSGCARAHRPHRLFSQRVSLAALAGALAWSATAFAAKPAL
                     TWPTTAFAAGPTLTLADAQHRAIEYSRQLVAQDFSVSASNNMAIAAGQLPDPVIKAGV
                     DNLPVNGSDQFSLTNDFMTTRYVGISQEITSGDKRELRARHYTLEAQKERAEKTVATA
                     SIERDTALAWLDRYYAEAMARVILEQQNQAKREIRAAESAYRGGQGSQADILAARSTL
                     "
BASE COUNT         1178 a         1478 c         1261 g         1131 t
ORIGIN      
        1 aacacttgaa taagaattgg caccgcaagc agcccgaaca tctaccccac aggtgactgc
       61 gccgatcagc cccaattcgt ctatgtcgcg gacagcggcc ggctgattgg cgtgcgggcg
      121 gtagcccaag aagcgggcga atgatccagg cagcggccct cgccattcac aaccgcatga
      181 ccgtgcggga attggctgac cagttgttcc cctatctgac aatggttgag ggactgaagc
      241 tcgcggcaca aaccttcttt aaagatgtca agcagctttc ctgctgcgct ggctaaaaaa
      301 agacgtgctt cggtgagtac tgatacggtt tccaggccaa cggtttaaga gccagcttaa
      361 tcaatggttt ccttcacaaa cgtcgaaaac tcaggtgtcg ctttcaaatc aaaccccatc
      421 ttttttgctg cctcaaaggt ttgttcaacg gtccagtttt gttggctcgc ttggttcaac
      481 gagtacagaa gcgctgcacg cgcccctgtt gcgcagtgaa gaagaaacgg gccatttttg
      541 ctcctcatca cggcgtcgag ttggcctatc tgatccacgg caagatggcc tttgttcacc
      601 ggaatattgg tgtaatccaa ccctgcgttt tttgtcagcg cctcgtttga cgccgtcgtc
      661 tccgccggca tccgaaaatc gatgactgat ttaatccctc gctgttttgc atcgtgaaga
      721 tcgtgctggg tcggctgcgg cccgataaaa acgttttctt cgacttgttt aaagttgctc
      781 atggcaatcc tttctggtga atatcagtga atatcagtca cccctgccgg ctctcgacca
      841 cacgagcgca gtacttccca agattttcag gtaaatgatc cggacaaacg ccgcaccgct
      901 tatttgctgg cacggcaaaa tcaatgaacg tcggatacgg caaattgagg ttcgccataa
      961 tttccctgaa ttgttccagt gtgcgttcct ggcccagtcg ggagttgtgc tgcttttcct
     1021 gcgcaatcga tgacacgtgc cgattgttgt aatcgtgtgc cggatcgaca agggtctcgt
     1081 ccggcaacga aaacaatttt tcatggacgc ttttatataa ggcgcccgcg ttgccgttct
     1141 gaaagtccgt gcggccacag ccatcaatga ggagcgcatc gccagtaaat atccggtcac
     1201 caaacaggta agcaaagtgt ccgtcggtat gccctggcgt gtgaagcggc tccaaccgaa
     1261 tactacccaa cacaaagggc tcgccttctt caattccgag atcagtgcaa ggcaaacgat
     1321 cgtaagcagg cgccgctatt ttgcttccca ccttcttttt taactcgagt gcagccgtaa
     1381 catgatccgc gtggatatgg gtatccacgg tatataccaa ggtcagtcca agaagattga
     1441 gctcaaacag atcacgatcc atggagacaa ccactggatc aatcaaaacg gcttgtcccg
     1501 tgtcctgaca acctaacaag taggtgtaag tactggacaa aggttcaaac aactggcgaa
     1561 atatcacggc gtcgtccttg tcagatgacg cgacctaggc cccaaccaga ggggtgacac
     1621 agggagtttc aggcccatca tcgcgtctgc acttttgaat caaaaatgtg agaacacctt
     1681 tggcgtgggc gatgcgtcga gatagtaaac gttgatggtc cccgtcttat ttcccgccat
     1741 ccccggggcg cccactggca taccgggaac agacagtccc ttgatcggag catgcgtctt
     1801 taatactcgc tggatgttct ctacggggac aagcccctcg aagacgtaac catcaatcaa
     1861 cgctgtgtgg cagccttcaa gtttttccgg cacggcatgc gcgcgtttta tcttggacaa
     1921 attatccgtg ttgatggttt gcacctcgaa gccattggac ctaaggtact gcgcataacg
     1981 gtcgcagcag ccgcagtcgg gattcttgta gagcgtcacc gacatccccg cggcaagcac
     2041 cggcaacggc aacgcaagca aggccgtgaa aaacaatttc gagatcgaca tttttatctt
     2101 tcctttgtaa acagtgtgac gcccgctcga ctactgcgcg ccatccttat tggtcacgac
     2161 ctggtccgcg tacttcgcaa cgatctcacc tactttttgc atgatctcgg cttgcatttt
     2221 taactgcagc ttctcatttc cggctggtaa ttgcggcatc atcgcgtgac ctctcatcat
     2281 gccgccgtcc attgcgccgc cccgcatcat gccgttgccg ccacccatca tgccactcat
     2341 cataccgtta cccatcatcc cgccctgcat cccgcatccg cgggagtcat ttcccatcat
     2401 gccgccttgc atcatcgacc gacccatatg tttcccattt gcgtcgggcc tattggcgtc
     2461 cgatccgttg cccatcgcgc cgccttgcat catgcccgca cccatgttgc cttgtgcgcc
     2521 ggtgttcgac ttgtcaagtt gccccgggct ctccgccgca agagcggata cgcccgcgct
     2581 tgcgatcaat gcagtaaaaa atacggcggc tatctgtttg cttttcattt gaaactcctg
     2641 aatcaattcg acctgtacat gcgtcggaaa cgacgggcta taagccccgt gtcataaacc
     2701 tgtgaagtgt tgggatccgt ccccgcacgt tgttcttgtg cggctcggta cttgcgttcg
     2761 accctgtcag atttgaaaac ggcaataccg aacagacaaa gaagaattcc gactgatgcg
     2821 gtgaatggcc gaaacctccg tcgtggccgc cacagcaggc caaaacaccc acgccaccca
     2881 cgcgggcgcg cgcgtcatgt acgccgcacc caccgaagga tcaggagaag aaaccttgga
     2941 cggagcaact attgcacttt gagcaaagca cgcgccgaca caggcagaca tacctacgcc
     3001 acaattaact tccgcaccaa aatgcatgct cacgtcttgg caaggagcac cattcgccac
     3061 tgtcataacg actgcgctta ccgacatcgg tggtgtgcta gcttgtgttg gcggcgccaa
     3121 tgaacccagc gcaaacatca agaaaatgat gtgcgccatg accattgaca ttcgatgcga
     3181 aacgtttctc atgatgaacc agtttattcc cgagcccccc gccattacaa gcctcattgc
     3241 agccttgttg gcacccaatg cactagcact tttcgacgaa catgtcgagt atgtaatgta
     3301 cccgtcagat agtgcgcgat ggatgacaaa aaagtaatgc gcgcgtcatc tcgctgtgcg
     3361 gttcgcgggc ataaagtaga ccttgatctg ggtaaaccag caccgcaacc aggcgcctca
     3421 gcggccggtt gcgacaacta cttttaaaag gaatctcatc atgtctctac gcaccacgac
     3481 cttaatggtt ctattcacca cagcgttggc tattccagtc tccagctttg cccagtggat
     3541 cccggataac agcgagctgt gcagtcataa cgttactaac acaacaagta tgaaaaccca
     3601 cgcgcaagtg cttcaagagc tacagcaggc taaggccgat cccagttggg cgactcgcca
     3661 agggcaagag acaggttcct ggcctgacaa aaacgctcaa cccgacaaaa ctcgcgaaca
     3721 ggtggagcat gcgctaaaca gcgtaacccc cgcgcagcgg gcctatcaac agagctttta
     3781 tgcgggcgca tgacgtcatt ggaacttccg cattccagtt ttagtcggag cgcgtgacgc
     3841 aaaagctaag ccacacgccg cctggtattc gaagcaaggc atgtataatt cggcatctaa
     3901 gaaaagtttg cccaaatgaa attatcccgt ggttctcggc ttaccgccgc gctcgtggcg
     3961 ctagtcagca tgctgttcat gcagatggcg ctggctggct atgcgtgtcc gggatataaa
     4021 acgggcctag gccatgacaa ggtcgcaatg gcacaaagcg caagcgacat ggcaatgcca
     4081 ggttgcgaac aagtcgatct ggcgcaaccc aatttatgcc aggcggctat ccagattgca
     4141 catcagtccc ttgacaaacc acaagtgcct cacgtacacg ccttcgtgcc agtgacgacc
     4201 atgttggtcg ccgaagcaag cgcactttat cgcccgatca ctggatcact taaagtggaa
     4261 cggccacagc gccacacagc gccgccaatt tccaccctac gctcccgctt acgtatttga
     4321 ccctctggat tgcttagttc gacatcgctg cgcttaacgc gcagccgatg ggttcgttgc
     4381 tatccggaga tttcatgtca ttgcccttca gcgggtgcgc gcgagcgcat cgcccgcatc
     4441 gcttattttc ccaaagagtc agtctggcgg ctctcgccgg cgcgctggcc tggtccgcaa
     4501 ccgcctttgc cgccaaaccc gcacttactt ggcccacgac cgcttttgct gcggggccca
     4561 cgcttacgct ggctgatgcg cagcatcgcg cgattgagta ctcacgccaa cttgtggcgc
     4621 aagacttttc ggtcagcgcc tccaacaaca tggcgattgc cgcgggccag ttgcctgacc
     4681 ctgtcatcaa ggccggcgtt gataatctac cggtcaatgg ttcagaccaa tttagtctga
     4741 caaacgactt catgaccacg cgttacgtcg gcatatcgca ggaaatcaca agcggcgata
     4801 agcgggaatt gcgtgcaaga cactacacat tggaagcgca aaaagagcgc gctgaaaaga
     4861 cggttgcaac tgcctccatc gaacgcgaca cggcgctggc ctggcttgat cgatactatg
     4921 ccgaggccat ggcgcgtgtc atattggaac aacaaaatca ggccaagcgt gaaattcgcg
     4981 cggccgaaag cgcttatcgc ggcggccagg gcagtcaggc cgacattctg gccgcgcgca
     5041 gcacgctg
//
LOCUS       DATCXU010000079         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_5167, whole
            genome shotgun sequence.
ACCESSION   DATCXU010000079 DATCXU010000000
VERSION     DATCXU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VIR04_01770"
     CDS             <1..809
                     /locus_tag="VIR04_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY9459508.1"
                     /translation="FWTFNNKVPGPFVRVRVGDTVTVNVTNAKDSSHIHSVDFHAVTG
                     PGGGAAVTQVPPGQTKSFTFKAMHPGLFVYHCATPMVAQHITNGMYGMILVEPEGGLP
                     MVDHEFYIMQGELYTAKPHGAGGLQEFSLEKLLAENPEHMIFNGSMDALTKIYNMKSK
                     VGDVVRIYFGVGGPNLTSSVHLIGEVFDRVYDQASLTSAPLTDVQTTLVPPGGAAMLE
                     FKTDYPGRYVLVDHALSRMEKGLAGFLTVEGKPDHSIFDSSEPVDPNSGH"
     gene            826..1575
                     /locus_tag="VIR04_01775"
     CDS             826..1575
                     /locus_tag="VIR04_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9459509.1"
                     /translation="MIFRQLFEPLSSTYTYLLACEETGQAILIDPVIVSMDRDLAEIG
                     RLGLTLAYSVETHIHADHITAARHLKEKTGSKIAAPAFDRLSCADVGIQEGIPFQVGS
                     IAIHPLHTPGHTAGHFAYFLDDRVFTGDSLLIEGCGRTDFQNGDTDALYKSVTEKLFV
                     LADETLVYPAHDYQDRRVSSIGQEKKRNPRLGQSRTPEQFREIMANLNLPYPKFIDYA
                     VRGNQECGVCPKDVPENLEKYCRQMTKSPQG"
     gene            1626..1940
                     /locus_tag="VIR04_01780"
     CDS             1626..1940
                     /locus_tag="VIR04_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY9459510.1"
                     /translation="MKGYQVTFFTVQDRRHDHKPVSDWLLELAKSMQLQGATLIGAET
                     GIGGRHRFHSTHFLELTDQPLEITMIVSEDECVRLFERLHAEPNLQLFYAKTPVEFGV
                     LG"
     gene            1995..2360
                     /locus_tag="VIR04_01785"
     CDS             1995..2360
                     /locus_tag="VIR04_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY9459511.1"
                     /translation="MLNFSIMHPEGILLIRPSAPLSKEDFDSLSAGVDAYLSGHAKLR
                     GVMIHAKAFPGWESFGAFTSHMHFVREHHKRVERIAVVTDSPLASMAELLAKHFTSAE
                     IRHFPFADDARALEWLQAT"
     gene            complement(2496..>3013)
                     /locus_tag="VIR04_01790"
     CDS             complement(2496..>3013)
                     /locus_tag="VIR04_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY9459512.1"
                     /translation="EPQSGKLWAIANERDEIGPDLVPDYMTSVQDGGFYGWPYSYYGQ
                     HVDTRVKPQRPDLVKKAIKPDYALGSHVAALGLLFSGDNALPAKYHNGAFVSEHGSWN
                     RSPLSGYRVVFVAFQQGKPVGVPEPVVTGFHSEDEKLLYGAPVGLAQDNDGAILIADD
                     VGNVVWRLTAR"
BASE COUNT          706 a          843 c          810 g          654 t
ORIGIN      
        1 ggttctggac cttcaataac aaggttcccg gtccctttgt gcgcgtgcgc gtaggcgaca
       61 cggtaacagt gaatgtaacg aacgcgaagg atagcagcca catccactcg gtggatttcc
      121 atgcggtgac agggccgggc ggcggtgcgg cagtcacgca ggttccgccc gggcaaacga
      181 aaagcttcac attcaaggcc atgcatccag gcctgttcgt ttatcactgc gcaacgccca
      241 tggtggcaca gcacatcacc aacggcatgt acggcatgat cctggtggag cccgagggcg
      301 gcctgcccat ggtcgatcac gagttctaca tcatgcaggg cgagctctat accgcgaagc
      361 cgcacggggc cggcggcctt caggaattct cgctggagaa actgctcgcc gaaaaccctg
      421 aacacatgat tttcaatggg tccatggacg cacttaccaa gatatacaac atgaagtcca
      481 aagtagggga tgtcgtacgc atttacttcg gagtaggcgg gccgaacctg acatccagcg
      541 tccacttgat tggtgaggta tttgatcgcg tctacgacca ggcctcgctg accagcgcgc
      601 cgctgaccga tgtgcaaacc acgctggtgc cgcccggcgg cgccgccatg ctggaattca
      661 agacggacta cccagggcgc tacgttctgg tcgatcacgc cctgtctcgg atggaaaagg
      721 gtctggccgg attcctgacc gtcgaaggca agcccgatca ttcaatcttc gacagcagcg
      781 agcccgtcga tccgaactcg ggacattgac gaacacgaga atcccatgat ctttcgtcaa
      841 cttttcgagc cattgtccag cacttatacc tatttgctgg cgtgtgagga aaccgggcag
      901 gcgattttaa tcgacccggt cattgtgtcg atggatcgcg atctggccga aatcggccgg
      961 ttggggttga cactcgctta cagcgtggaa acccatattc atgctgatca cattaccgct
     1021 gcgcgccatt tgaaagaaaa gacgggcagc aaaatcgccg cgccggcttt cgatcgtctg
     1081 tcgtgtgccg atgtcggaat ccaggaaggt attccattcc aggttggcag tatcgcaatc
     1141 caccctctgc atacacccgg acataccgcc ggacatttcg cttattttct ggacgatagg
     1201 gtctttaccg gcgactcgct gctaatcgaa ggctgtggcc gcaccgattt ccaaaatggc
     1261 gacacggatg ccttatataa gagtgtcacc gaaaaattat tcgtgttggc ggacgagaca
     1321 ctcgtttatc cggcgcacga ctaccaggac cggcgcgttt cctcaatcgg gcaagaaaag
     1381 aagcgcaacc cgcggcttgg tcagagccgc acgccagagc agtttcgcga gatcatggca
     1441 aatctgaatc tgccatatcc caagttcatt gattatgccg tgcgcggcaa tcaggaatgc
     1501 ggcgtttgcc cgaaagacgt gcccgagaat ctcgaaaagt attgcagaca aatgaccaaa
     1561 agcccgcaag gctagggatt caactgaatt gccctggcaa agatctgtag tcaggaggtg
     1621 gtgtgatgaa aggctatcaa gtgacatttt tcaccgtaca agatcgccgt cacgaccata
     1681 aaccagtaag cgactggctg cttgaactgg ccaaatccat gcagcttcaa ggcgcgacgc
     1741 tcatcggtgc agaaacaggt ataggtgggc gccacaggtt tcactcgact catttccttg
     1801 agttgaccga tcagcctctt gaaatcacca tgattgtgtc tgaagatgag tgcgtcaggt
     1861 tgttcgaacg gctccatgca gaaccaaatt tgcaattgtt ttacgcaaaa acaccggttg
     1921 agtttggggt gctcggttaa taccgcaaaa aacgaacttt gagtcgtaaa aaagctcggc
     1981 tattggagag tcaaatgctc aacttttcca tcatgcaccc cgaaggtatt ttgcttatca
     2041 ggcccagcgc gcctctaagc aaggaagact tcgacagtct cagtgccggc gttgacgctt
     2101 atctgtccgg gcatgccaaa cttcgcggcg taatgattca tgcaaaagcc tttcccggat
     2161 gggaaagttt tggcgcattc acatcccaca tgcactttgt ccgcgaacac cataagcggg
     2221 tcgagcgcat tgccgtggta accgacagtc cacttgccag catggcggaa ctgctggcca
     2281 aacacttcac atcagccgaa atcaggcatt ttccctttgc cgacgacgcg agggcgttgg
     2341 agtggttgca ggccacctga ggggggtgag gttcggaatc agttcaaacc cggccgggcg
     2401 aaataaattt attaatacgc ataatgtata ttatgtcaag ttatgtgatg tgcgattgga
     2461 accaccccct gccgatccga agtaatcgtc cgatactagc gcgccgtcaa acgccacacc
     2521 acattgccca catcgtcggc gatcaggatc gcgccgtcgt tatcctgcgc cagtccgacc
     2581 ggtgctccgt acaacagttt ctcgtcctcc gaatggaaac ctgtgacaac gggctcgggg
     2641 acgcctaccg gcttgccttg ctgaaaggcc acaaagacta cgcggtagcc gctcagcggc
     2701 gatcgattcc agctaccgtg ttcgctgacg aatgcgccat tgtgatattt tgccggtagc
     2761 gcattgtcgc ccgaaaacag caaccccagg gcagcgacat gcgatccgag cgcgtaatct
     2821 ggcttgattg cttttttgac cagatcgggt cgttgcggct tcacgcgagt gtccacatgc
     2881 tgcccataat agctgtaggg ccacccatag aagcctccgt cttgcacaga ggtcatgtaa
     2941 tcaggcacca gatcggggcc gatctcgtcg cgctcattgg caatggccca cagcttcccg
     3001 ctctgcggct ccc
//
LOCUS       DATCXU010000080         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_344411,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000080 DATCXU010000000
VERSION     DATCXU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344411"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VIR04_01795"
     CDS             <1..405
                     /locus_tag="VIR04_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HEY9459513.1"
                     /translation="LPQLAEEAELPDEELFPTYEALGLLGLAHVDKGDIALTPLGRRY
                     AEAEQTLRQEIFGQQLLTHVPLAAQIRQQLENEPAGTLSEEPFIKLLEQFLEADEAKR
                     ILEIAIEWGRYGEVYEYDFHTGQLKLPDQIEE"
     gene            425..1315
                     /gene="nadE"
                     /locus_tag="VIR04_01800"
     CDS             425..1315
                     /gene="nadE"
                     /locus_tag="VIR04_01800"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEY9459514.1"
                     /translation="MVLIWHIVSNPANPPKGEIMLSAEQHSVQHEIIKLLRVDASFDP
                     AAEVERRTHFLADYLISTKRSTLVLGISGGVDSLVGGLLSQRAVNQVRAQGAKANFVA
                     MRLPYGEQRDEHDAIACLETIKPDSIITVNVQPASDAMLAMLQQSDVSFRDAAHRDFI
                     LGNIKARQRMIAQYAVAGAYGGLVVGTDHAAEALMGFFTKFGDGAADITPLAGLNKRR
                     VRALAASMGAPDALVCKVPTADLESLSPLKPDEDAFGVKYEDIDNFLEGKDVSEEAFE
                     IIVRTYRNSAHKRAQPVAPL"
     gene            1343..1897
                     /locus_tag="VIR04_01805"
     CDS             1343..1897
                     /locus_tag="VIR04_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY9459515.1"
                     /translation="MTRTSPIPPMSAWLFVLSLAAPGCIPVAFADNTSQGAVSLRAGA
                     GEHASRIGLAWESPPLWSYRFGGNKNRLDLTTELGVAYWKAKTPRTPASVWQFSAMPV
                     LRWTTGERFYIEAGFGPTVFTRTHFANEEISTAFQFGSEVGMGVYLSRASRIGMRFAH
                     FSNGGIKTPNPGFQILQLTYTYQY"
     gene            2065..2505
                     /locus_tag="VIR04_01810"
     CDS             2065..2505
                     /locus_tag="VIR04_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY9459516.1"
                     /translation="MLYRAQFPQGIFMDLDRLQREMQQAFNFPSNIRGRSWDTYPATN
                     VGHTPNSVEIFVFLPGVEPSQIELHLEKGVLVVAGQRNIDVADKDEKAVMHIEERFAG
                     QFRRVVTLPDDIDPNQVDARYRDGVLHICIQRREAATPRQITIQ"
     gene            2544..2924
                     /locus_tag="VIR04_01815"
     CDS             2544..2924
                     /locus_tag="VIR04_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY9459517.1"
                     /translation="MSDTNVVERKPETDREPAPLLPAVDVYEDSRGITLHADFPGVPK
                     ENLSLQIKDETLVIEGTIDLNTPESMEIVHAEIDRSRYLRTFSLSKELDAEKIDAHFE
                     AGVLTLHIPRAEHAKPKRIEVKVA"
     gene            complement(3001..3264)
                     /locus_tag="VIR04_01820"
     CDS             complement(3001..3264)
                     /locus_tag="VIR04_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459518.1"
                     /translation="MPLSTGLLSAREQGSILRYPLEGLYVLAIANDEICGGEQAYGPN
                     DYMEQAIGLLRHHCEADIEIYRADIVKGVLINPIRTVPLESDV"
     gene            complement(3371..>3594)
                     /locus_tag="VIR04_01825"
     CDS             complement(3371..>3594)
                     /locus_tag="VIR04_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459519.1"
                     /translation="GADGDNYRQYARGILSRLCSSEFLGKREEGWQGIIKHASYHQGN
                     KLGVNESVMWGDYYFVEALHRLAQAAPNA"
BASE COUNT          856 a         1019 c          978 g          741 t
ORIGIN      
        1 ctgccgcaac tcgcggaaga agccgaactg cccgatgaag agttattccc gacttacgaa
       61 gcccttggcc tgcttggcct ggcgcacgtc gacaaaggcg acatcgcgtt gacgccgctc
      121 ggacggcgat acgccgaggc cgaacaaact ctcaggcaag aaatttttgg acaacaactg
      181 ctgacgcacg tacctttggc cgcgcagatc cgccagcaac tggaaaacga gcctgccggc
      241 acactgtccg aggaaccctt catcaagctg ctggagcagt ttctggaggc tgatgaagcc
      301 aagcgcattc tggaaatagc gattgaatgg ggccgttacg gggaagtgta tgaatacgat
      361 ttccatactg gccaattgaa gttgcccgat caaatcgagg aataatcacc taccaatatg
      421 gatcatggtg ttaatctggc acatcgtcag caaccccgcg aatccgccca aaggagaaat
      481 catgctgtca gcggagcaac atagcgttca acacgaaatc atcaaattgc ttcgagttga
      541 tgcaagcttt gatcctgccg ccgaagtcga acgacgcacg cacttcctgg ccgactattt
      601 gatctcgaca aaacgctcga cgctggtgct cggcattagc ggaggcgtag attcgctcgt
      661 cggcgggctt ttgtctcaac gcgcggtaaa ccaggtccgc gcccagggcg ccaaggcaaa
      721 tttcgtggca atgcgccttc cgtacggcga gcaacgagac gagcacgatg cgattgcctg
      781 tcttgaaacc atcaagcccg acagcatcat cacagtgaat gttcagcctg caagcgacgc
      841 catgcttgcc atgttgcagc aatcggatgt cagctttcgc gacgcggccc accgcgattt
      901 tattttggga aatatcaagg cccgtcagcg catgattgcg caatacgccg ttgcaggcgc
      961 ctatggcggt ctggtggtgg gaacagacca tgccgccgaa gcattgatgg ggttttttac
     1021 caagttcggg gacggcgcgg ccgatatcac tccgctggcc gggctgaaca aacggcgcgt
     1081 ccgggcgctg gcagcctcca tgggcgcgcc cgacgcactg gtctgcaaag tgcctacggc
     1141 ggatctggaa tccctttcgc cgctaaagcc cgacgaagac gcgttcggcg tgaaatacga
     1201 agacatcgac aatttcctgg aaggcaaaga cgtcagtgag gaggccttcg agattatcgt
     1261 acgcacctac cgaaactcgg cccataagcg ggcgcagccg gtagcgccgc tatagacgtg
     1321 atcgcactaa gaggacaatt caatgacacg cacgagcccg ataccgccaa tgtcagcctg
     1381 gttgttcgtt ttgagccttg cggcgccggg ctgcatccca gtcgcgttcg ccgacaatac
     1441 ttctcaaggc gctgttagtc tgcgtgccgg ggcaggagag cacgccagcc gtattgggct
     1501 tgcctgggag tccccgccgc tttggtccta ccggtttggc ggcaataaaa atcgactgga
     1561 tctgaccact gaactgggcg tggcttactg gaaagcgaaa acgccacgta ctccggcgag
     1621 tgtatggcag tttagcgcca tgccggtgtt gcgatggacg acgggcgagc gattctacat
     1681 cgaggcgggc ttcggaccga cggtattcac ccgtacgcat ttcgcgaacg aagagatcag
     1741 tacggcattc caattcggca gcgaagttgg aatgggcgtc tatctttccc gcgcgagccg
     1801 gataggcatg cgttttgcgc atttttcgaa cggcggtatc aagacgccca accccggctt
     1861 ccagatactg caactgacgt acacctatca atactaatac acacgtcgcc ggccatcagg
     1921 cggggccggt caaccggctt gcggaacact cggttacctt ttcaaaagca ttgaatttct
     1981 tcacgccgca tccatattga atatgtgagc gggaacgctt cccgtctgat ggatgttttg
     2041 ccgcaacttt gacaggagat agcaatgcta tacagagcgc agtttccaca aggcattttt
     2101 atggacctgg atcgtcttca gcgcgaaatg cagcaggcct ttaatttccc ctccaatata
     2161 cgggggcgaa gctgggacac atacccggcc accaacgttg gccacactcc caattcagtc
     2221 gaaatcttcg tgttcttgcc gggcgtcgag cccagccaga tcgagcttca tctcgagaaa
     2281 ggcgtgttgg tggtggccgg gcagcgaaat atagacgtgg cggacaagga tgaaaaggca
     2341 gtcatgcata tcgaggagcg cttcgcaggg cagtttcgac gcgttgttac gctgcccgat
     2401 gacatcgacc cgaatcaggt cgacgcccgc taccgcgacg gcgtattgca catatgcatc
     2461 cagcgccgtg aggcagccac cccgcgccaa attaccattc aataagacgc cgcccgtaat
     2521 cgcaacgaaa aaggaaccga atcatgtcag atacaaatgt tgttgaaaga aaaccggaaa
     2581 ctgatcgcga gccagcgcca ctgctcccag ccgtggacgt atacgaagat tctcgcggca
     2641 tcacgctgca tgctgatttt ccgggcgtac ccaaagaaaa cctgtcgctg caaattaaag
     2701 acgagactct ggtcatcgag ggaaccatcg acctgaatac ccccgagtcc atggaaatcg
     2761 tgcacgccga gatagaccga tcacgctatc tgcgcacgtt ctcgctatcg aaagaacttg
     2821 atgccgaaaa aattgacgcc cacttcgaag caggggttct gaccttgcat attccgagag
     2881 ctgaacatgc aaaaccaaaa cgtattgagg tgaaagtcgc ctgacgaagc atgcgcgggt
     2941 aatgtgcgcg ttacgccggg acggcggcaa ccgtacccaa cgtggcgcgc gtattagcca
     3001 tcagacgtca gattcgagcg gaacggtgcg aatcgggtta attaaaacgc ccttcacaat
     3061 gtcggcgcgg tatatctcga tgtcggcttc gcagtgatgg cgcagcagcc ctatcgcctg
     3121 ctccatgtaa tcattggggc catatgcctg ctcaccgcca caaatctcat cgttggctat
     3181 ggcaagcaca tacaagccct ctaacgggta tcttagaatt gaaccctgtt cccgcgcgga
     3241 taacaagcca gttgaaagcg gcatcccaag ctcccgttta aaaggtcatc ccaatagctt
     3301 ccggtccagt aactgtatcg cgggagcctg ggaataaaac gcctgagcaa cggggctttt
     3361 agtgttctct ttacgcgttc ggagcagcct gggccaacct gtgcaaagcc tccacaaagt
     3421 agtaatcgcc ccacataacg ctttcattta cgcccagctt attgccttgg tgatagcttg
     3481 catgtttaat gattccttgc cagccttctt cccgcttgcc aagaaactct gagctgcaaa
     3541 gccgggacag aattccgcgg gcgtactggc ggtagttgtc gccatcggct cccg
//
LOCUS       DATCXU010000081         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_837389,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000081 DATCXU010000000
VERSION     DATCXU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837389"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VIR04_01830"
     CDS             complement(<1..245)
                     /locus_tag="VIR04_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY9459520.1"
                     /translation="MKPIGSLHLIPVGLGEAPTSVWLPQAVQELAGRLDTYIAENART
                     ARAFLKLTGTKLPLREITIHTLDEHVDTAQMRQWLIPL"
     gene            complement(242..829)
                     /locus_tag="VIR04_01835"
     CDS             complement(242..829)
                     /locus_tag="VIR04_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEY9459521.1"
                     /translation="MRLILASSSRYRRQLLTRLHCPFDVISPNIDETPLPGEVPRALA
                     LRLSAAKANAVTRDHPDALVIGSDQVAAIDGAFIGKPGTFERAQAQLRLLSGRTVEFH
                     SGLCVSNGARHESVDVITECRFRRLSPEEIDAYLRLDEPFDAAGSAKAEGLGICLMES
                     IQSDDPTAIIGLPLIALSRLLRSFGLNPLTRSEAS"
     gene            913..1446
                     /locus_tag="VIR04_01840"
     CDS             913..1446
                     /locus_tag="VIR04_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY9459522.1"
                     /translation="MNAPVQYLDTFELARLGLKVKGQTPLSHFSRLLADLPAQADSVV
                     TWSVVGEHSASGQFFLRVRAKGAVTLECQRCLAPLEWQVDTLSRLLVVSTEADLGDGG
                     HEDAEAAQDSIERIVGSHRFDILTLIEDEIILVLPYVPKHDVCPAALNRLVPSEDSQA
                     DEAAPSPFAVLGRLKKN"
     gene            1510..1692
                     /gene="rpmF"
                     /locus_tag="VIR04_01845"
     CDS             1510..1692
                     /gene="rpmF"
                     /locus_tag="VIR04_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY9459523.1"
                     /translation="MAVQQNKKSSSKRDMRRSHDFLTAPATAIEPTTGELHLRHHISP
                     TGFYRGRKVLKTKADE"
     gene            1735..>2489
                     /gene="plsX"
                     /locus_tag="VIR04_01850"
     CDS             1735..>2489
                     /gene="plsX"
                     /locus_tag="VIR04_01850"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY9459524.1"
                     /translation="MPITEIRIAVDCMGGDAGLPVTIPAACTFARKHPDTRFLLAGDS
                     ASIRASLVSLGCANESWYQILPASEVVGMSDSVEIALRRKKDSSMRVAVQAVKDGAAD
                     ACISAGNTGAWMAISRYVLKTLEGIDRPAIATSLPNQTGGATTMLDLGANVDCSAQNL
                     LQFAIMGAALASTVDHRERPTVGLLNIGEEVIKGNEIVKQAAELLRSSGLNFYGNVEG
                     DDIFKGTVDVVVCDGFVGNVVLKSAEGLARMVAT"
BASE COUNT          513 a          620 c          767 g          589 t
ORIGIN      
        1 agcggaatca gccattggcg catttgggcg gtatcaacat gttcatcgag tgtatggatg
       61 gtgatttccc gcaggggcag tttcgtgcct gtgagtttta gaaaagcgcg tgcagttcgc
      121 gcgttttccg cgatgtaggt gtcgagccgt cccgccagtt cttgcactgc ctggggcagc
      181 cagacggagg tgggcgcttc ccccagaccg acagggatca aatgcagtga gccaattggt
      241 ttcacgaggc ctcggagcgg gttaggggat tgagtccgaa tgagcgcagc agacggctca
      301 gggcgatgag cggcagaccg ataatggccg tggggtcgtc ggactggata ctttccataa
      361 ggcaaattcc caagccttcg gcctttgcgc tgccggccgc atcaaaaggc tcgtcaagac
      421 gcaggtaggc gtcgatttcc tccgggctta gccggcggaa tcggcattcc gtaattacgt
      481 cgactgactc gtggcgcgct ccgttgctga cgcacaggcc gctgtgaaat tccactgtcc
      541 ggccgctaag aagcctgagt tgcgcttgcg cgcgctcgaa cgtgccgggt ttcccgatga
      601 aagcgccatc gatggcggca acttggtccg accctataac gagcgcatcg ggatgatcgc
      661 gcgtcacggc gttggccttg gccgcggaca ggcgcaaggc cagtgcgcga gggacttcgc
      721 caggcagtgg cgtttcgtcg atattgggcg aaatgacgtc gaacgggcaa tgcagacgag
      781 tcagcagttg gcggcgataa cgtgagcttg aggcaaggat aagacgcatg gactgaattt
      841 tagcgtgtta atttgactgt aaacaggctc gcacgttatc atttgatgtt ttcctgcctt
      901 aaatcgtcta cagtgaatgc gcctgtacag tatcttgaca cgtttgaact tgcccgtttg
      961 ggcctgaaag taaaaggaca aacgccgcta agccattttt cgcggttgct ggccgattta
     1021 ccggctcagg cggattcagt cgtaacgtgg tcagtcgttg gcgagcatag tgcgtcgggg
     1081 cagttttttt tgcgcgttcg ggcaaaaggg gctgttacgc tcgaatgcca gcgttgtctg
     1141 gcgccgctgg aatggcaagt cgatacactt agccgtttgc tggtggtttc aaccgaggcg
     1201 gatttgggcg atggtggcca tgaagacgca gaggcggcac aggattccat cgagcggatc
     1261 gtgggttcgc atcgcttcga tatactgacg ctgatcgaag acgagatcat cctggttttg
     1321 ccttacgtac ccaagcatga tgtgtgtccg gcggccctga atcgtctcgt gccatccgaa
     1381 gattcccagg ctgacgaagc tgctccttct ccgttcgcgg tgttgggccg gctgaaaaag
     1441 aattgaattc attaagatta atttgcggga ttcatcgcgt acaatagcga cccgtttcca
     1501 ggagttgcca tggctgttca gcaaaacaaa aaaagttcat ccaagcgcga tatgcgccgt
     1561 tcgcacgatt tcttgacggc gcccgcgacg gctatcgagc cgacaaccgg tgaactgcat
     1621 ctgcgtcatc atattagtcc cacggggttc tatcgcggcc gtaaagtgct taaaaccaaa
     1681 gccgacgaat aatcttagtt tgtcttgcga tctagctcat agattgcact cctgatgccg
     1741 attaccgaga ttcgaatagc tgtcgattgc atgggcggag atgccggctt gccggtaacc
     1801 atccctgcgg cgtgcacgtt tgcccgcaag cacccggata cgcgtttttt gctcgcgggt
     1861 gactccgcct ccattcgtgc cagtctggtg tcgcttgggt gtgccaatga aagctggtat
     1921 caaatattgc cggcctctga agtggtcggc atgagcgatt cagtggaaat tgctttgcgg
     1981 cgcaagaaag attcgtcaat gcgtgtggcg gtgcaggctg ttaaagacgg tgcggccgac
     2041 gcttgcattt cggcgggcaa tacgggcgcc tggatggcga tttcgcgcta tgtgttgaaa
     2101 acgctcgaag gaattgaccg gcccgctata gcgacgtcgc tgcccaatca gactggcggc
     2161 gccacgacca tgctggatct gggcgccaat gtcgattgtt cagcacagaa tttgctgcaa
     2221 tttgcgatca tgggtgcggc cctggcaagc acggtcgatc atcgcgaacg gccgacagtt
     2281 ggcttgctta atattggtga agaggtcatt aaaggcaatg aaatagtcaa gcaggcggcc
     2341 gagttgttac gttccagcgg cttgaatttt tatggcaacg tcgaaggcga cgatattttc
     2401 aagggcacgg tcgacgtcgt ggtttgcgat ggttttgtgg gcaatgtggt tcttaaatcg
     2461 gccgagggtc tggccagaat ggtggctac
//
LOCUS       DATCXU010000082         4683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_851035,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000082 DATCXU010000000
VERSION     DATCXU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851035"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VIR04_01855"
     CDS             <1..391
                     /locus_tag="VIR04_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459525.1"
                     /translation="RHAAYKCYVFVDPSQLKPGWNRDRIQSEINARGVPCYSGSCSEV
                     YLEKAFDNTGWRPPQRLTIAKELGETSLMFLVHPTLTDAEIDKTCRVLEEVMKTCAAT
                     APKRQIAEQSRASRRPEQNAASYEATR"
     gene            375..1406
                     /locus_tag="VIR04_01860"
     CDS             375..1406
                     /locus_tag="VIR04_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY9459526.1"
                     /translation="MKQRDDRNTSNAGIAVKHGPGILTISLDFELYWGVRDKRTLAAY
                     SQNLAGVHQAIPQILALFDKHGIHATWATVGLLFCRDAHEAMTRYAPDTRPNYESANL
                     SPYAYLQNGQNLDAKYHFAPELIDLIASHAHQEIATHTFSHYYCLEKGQTLEAFKADI
                     EAAKVLARERNLETSSIVFPRNQWNQDYLSALNELGIKCYRGNERSWVYRAVENEEQS
                     LLRRAGRLVDTYFNLCGHQTYPLIDQNPNMPYNFPSSRLLRAYKKKLAPLEGLRLRRI
                     RKSMLHAAKNGEIFHLWWHPHNFGVDIRENMKFLAKVLQEFERLHSSYGMQSANMAEL
                     YRLKKSAYE"
     gene            1399..2175
                     /locus_tag="VIR04_01865"
     CDS             1399..2175
                     /locus_tag="VIR04_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HEY9459527.1"
                     /translation="MNKKRTRIIMLCGPGQSSKIMYNGLAQRFDVELVISEDKPSSWR
                     LLKRRIKNLGILKVLGQLCFMAFNRLVLQRSQKRRIAELMELYHLDASAPPETVTQHV
                     DSINAESVMATLKRRDPDAVVVNGTRIISGKVLSCIDKPFINTHVGITPRYRGVHGGY
                     WALANRDPLNCGVTVHLVNTGIDTGGVLYQRTISPEKKDGFNTYPIHQIAAAIPLMRS
                     ALLDVSNLELKVTPGVAPSQLWYHPTLVEYLKARILFGVK"
     gene            2353..4473
                     /locus_tag="VIR04_01870"
     CDS             2353..4473
                     /locus_tag="VIR04_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY9459528.1"
                     /translation="MSFIVTPGKPAVTPLMPIKAVGIREYLLALSRKQKRVLQLSVDT
                     LLIWFALWLAFYLRFGDAGMIRPAGGHAWIFVLAPILALPIFARLGLYRAVVHCLGYQ
                     ALAALALAITASSILLACAVYLYVEPPDIVPRSIVFIYWMLCLLLIGGLRIAMRQYFG
                     HAPLPHLNTATLFKAKNKNFDSKIRVVIYGAGAAGTQLLTALGQGRERHVVAFIDDDP
                     QLTERVVAGVLVYASADLGRLVKETRPQEVLLAIPSAPRSRRAKIINLLAPYPLHVRT
                     VPGLTDLASGRVKAEDLQEVNIADLLGRDPVKPNEKLFRRCIRDQTVMVTGAGGSIGS
                     ELCRQIISIGPRILILFEHSEFNLYSIENELNAYVRRTNLSVRIVPILSSVRNQKRLL
                     NIMTIWKVDTVYHAAAYKHVPMVEHNVAEGIVNNVFGTLYCAQASVLAGVRHFVLIST
                     DKAVRPTNIMGGTKRLSELVLQALAEEPMPSLYGRTSQPAVPNRTRFTMVRFGNVLDS
                     SGSVIPLFRRQIRAGGPVTVTHPEITRYFMTIPEAAQLVIQAGAMGKGGDVFVLDMGE
                     PIKIAELAQKMILLSGLSIRSPGNKDGDIAIEFVGLRPGEKLYEELLIGDSATATDHP
                     MIMRASEKHLDWATLKISLTEIAAAVMEDDYLRVRELFCQLIDGYTPSADMADWIHQQ
                     QMTIASQHAPQRPLKVEPADVNAS"
BASE COUNT         1157 a         1272 c         1188 g         1066 t
ORIGIN      
        1 ccgccacgcg gcgtacaaat gttatgtctt cgttgatccg tcgcaactga agccgggttg
       61 gaaccgcgac cggatacaaa gtgaaatcaa tgcgcgaggc gtgccctgct attcaggctc
      121 atgttccgag gtgtatctgg aaaaagcctt cgacaatact ggttggcggc cgccacaacg
      181 tttaaccata gccaaggagc tgggtgaaac ctccttgatg tttctggtgc atccaacgct
      241 gaccgatgct gaaatcgaca aaacctgcag ggtgctggaa gaagtaatga aaacttgtgc
      301 ggccacagcg ccaaagcgcc aaatcgcgga acaatcaaga gcatccaggc ggcctgaaca
      361 aaatgccgcc agttatgaag caacgcgatg accgcaatac gtccaatgcc ggaatcgccg
      421 tgaaacacgg ccctggcata ctcacgatct ctcttgattt tgagctttac tggggtgttc
      481 gcgacaaacg gacacttgct gcatacagcc agaatctggc cggggtacat caggcaatcc
      541 cgcagattct ggctttgttc gacaaacatg gcattcacgc gacttgggca acggtaggtc
      601 tgttattttg tcgagatgct catgaagcga tgacacggta tgcacccgac acacgtccga
      661 attatgaatc agccaatctc tcgccctacg cctatttgca aaacggccag aatcttgatg
      721 ccaaatatca cttcgcgccc gagttgatcg acctgattgc ttcgcacgca caccaggaaa
      781 tcgcgacgca cactttctcg cactattatt gccttgaaaa aggtcagacc ctggaagcct
      841 tcaaagcgga tatagaagcc gccaaggttc tcgcgagaga aaggaatctg gagacctcca
      901 gcattgtttt tccacgcaac caatggaacc aggactatct ttctgcgctg aatgaactgg
      961 gcatcaagtg ctatcgcggc aacgaaagaa gctgggttta ccgggcagtg gaaaacgaag
     1021 aacaaagcct tcttcggcgt gcaggcagac ttgtagacac atatttcaat ctgtgcggtc
     1081 atcagaccta cccgttgata gaccagaacc caaacatgcc ttacaacttt ccgtcaagca
     1141 gacttctgcg cgcttacaaa aaaaagctgg cccctcttga aggattgcga ctaagaagaa
     1201 tacgaaaatc catgctgcat gccgccaaaa acggcgagat cttccatctt tggtggcatc
     1261 cgcacaactt tggtgttgat atccgtgaga atatgaaatt cttggccaaa gtgctgcaag
     1321 aattcgagcg attgcacagt agctacggga tgcagtccgc aaatatggca gaactttacc
     1381 ggctgaaaaa atcggcttat gaataagaaa agaaccagaa taataatgct gtgcggcccg
     1441 ggccagtctt caaaaataat gtacaacggt ttggcgcaaa ggttcgatgt agaactggta
     1501 atctccgaag ataagccctc ttcatggcgc ttgctcaagc gaagaatcaa aaatttggga
     1561 atcctgaaag ttctggggca gttgtgcttc atggcattta atcgcctcgt gctgcaaagg
     1621 tcccaaaagc gccgaatagc ggaactcatg gagctctatc atcttgatgc aagcgcacct
     1681 cccgaaacgg tgacgcagca cgtcgacagc atcaatgcgg aatcggtaat ggccacattg
     1741 aagcggcgcg atccagacgc agttgttgtc aatgggaccc gcattatttc cggcaaagtg
     1801 ctgtcttgca tcgacaagcc atttatcaat acccatgtgg gcatcacgcc tcgttaccgc
     1861 ggcgtccacg gcggctattg ggcactggcc aaccgtgatc ccctgaattg cggtgttacc
     1921 gtacatttgg ttaatacggg aatagatact ggcggcgttc tttaccaacg caccatctcg
     1981 ccagagaaaa aggatgggtt caacacttac cctatacacc aaatcgcggc agcgattcct
     2041 cttatgcgat cggcactgtt ggatgtatcg aacctcgaac tgaaggtgac accgggagta
     2101 gcaccctctc aactgtggta tcaccctaca cttgtcgagt acctgaaagc gcggatcttg
     2161 ttcggcgtca aataacgcat caggtacctg gttgcggtac ccgccgccgg cagctaactg
     2221 ggccaagcgt attaacccga acgtcgtcta tcttgcagag gcttcggcct ctatcattta
     2281 ttttgtcgct tatgttcaag atgctagtaa acggccactt caagctgtaa aaaaatatta
     2341 gcagggaaca agatgtcttt tatcgttaca ccggggaaac cggcggtgac ccctctgatg
     2401 ccgatcaaag cggtcggtat ccgcgaatat ttgctggcct tgtcgcgcaa gcaaaaacgt
     2461 gttttgcagc tttcggtcga cacgttgcta atctggtttg ccttgtggct ggccttttac
     2521 ttgcgcttcg gagacgccgg catgatcagg ccggcaggcg gccacgcgtg gatattcgtt
     2581 ctcgcgccca tactggcatt gcctatcttc gcacgactgg gattgtaccg tgcggtggtg
     2641 cattgcctgg ggtatcaggc gctggcggcg ctcgcgctgg caattaccgc atcgtccatt
     2701 ctactagcct gcgcggttta cctttatgtc gaaccgcccg acattgtccc ccgctccatc
     2761 gtgttcattt attggatgct atgcctgctt cttatcggcg gactgcgcat cgccatgcga
     2821 cagtactttg gccatgctcc actgccgcac ttgaatactg ccacgctttt caaagcaaaa
     2881 aataaaaatt tcgactcaaa aatccgcgtc gtcatttacg gcgcaggtgc ggccggcacg
     2941 caattgctga cagccctggg ccaaggccgg gaaagacatg tagtggcctt tatcgacgac
     3001 gatccgcagt tgactgaacg cgttgttgca ggagtgcttg tctatgcatc cgcagacctc
     3061 ggccgcctcg taaaagaaac aaggccgcaa gaggtcctgc tggcgattcc ttccgccccg
     3121 cggagccggc gagcaaaaat aatcaatctg ctggctcctt atccgctgca tgtgcgcacc
     3181 gttccagggc ttacggatct ggcaagcggc cgcgtcaagg ccgaagatct gcaagaggtg
     3241 aacattgccg acctgctcgg acgcgatcca gtaaagccta acgaaaaatt gttccggcgc
     3301 tgcatacgcg atcaaacggt catggtcacc ggagcgggcg gctcgatcgg ctcggagttg
     3361 tgccgccaga tcataagcat tggcccgcgc atactgatcc tgtttgagca ttccgaattt
     3421 aacctgtata gcatcgaaaa cgaattaaat gcctatgtgc gccgaacgaa tttgagcgtc
     3481 aggatcgttc ccatactcag ctcagtgcgc aaccagaagc gtttgttgaa cattatgaca
     3541 atctggaagg ttgacacggt ctaccatgcc gccgcctaca aacatgtgcc catggtcgaa
     3601 cataatgttg ccgaaggtat cgtcaacaat gtctttggca ccctgtactg cgctcaggct
     3661 tccgtcttgg ccggggtccg ccatttcgtg ctgatttcca ccgacaaggc agtcaggcct
     3721 accaacatca tgggcggcac caagcgtctg tcggaactgg tcttgcaagc gttggcggaa
     3781 gaacccatgc caagcctgta tggcagaacg tcgcaaccgg ctgtgccgaa ccgaacccgt
     3841 tttactatgg tgcgttttgg caatgtgctg gactcgtcag gttccgtcat tccgcttttt
     3901 cgccgccaaa ttcgcgccgg gggccctgtc actgtcacgc atcctgaaat tacccgctac
     3961 ttcatgacca ttcccgaggc ggcacagctc gtcatccagg ccggagccat gggcaaagga
     4021 ggtgatgtct tcgtactgga catgggcgag ccaatcaaaa tcgccgaatt agcccaaaag
     4081 atgattttgt tgtcgggctt gagcatacga tcacccggca acaaggatgg cgatatcgcc
     4141 atcgaattcg tcggcctgcg ccccggtgaa aaactctatg aagaattgct cattggcgac
     4201 tccgctacag ccaccgatca tccgatgata atgcgagcga gcgagaagca cctggattgg
     4261 gcaacgctta aaatctccct gaccgagatt gccgccgccg taatggaaga cgactatctg
     4321 cgtgtgcgcg aattattttg ccaactgatt gacggctata ctccttcagc ggacatggct
     4381 gactggattc atcagcaaca aatgacaatc gccagccagc atgcgccgca gcgtccgctt
     4441 aaagtcgaac ccgctgacgt caacgcctca taaacgaaac aacatgtaaa taaaccagcc
     4501 gaaagcgcgt tattgttgcc ttttgaagcg ctttggcgtg cacaacttac gtttgcatgc
     4561 gcaatttcga ccacaattga cttgtcgctt aaacagacaa ggactcgaat gaaacgatta
     4621 ctactgatag gggctgctgc actcgcctta gcttgttcca gctccgcaat ggctcgcgtt
     4681 gac
//
LOCUS       DATCXU010000083         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_579740,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000083 DATCXU010000000
VERSION     DATCXU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579740"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VIR04_01875"
     CDS             <1..797
                     /locus_tag="VIR04_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459529.1"
                     /translation="SVGEPINPEAWMWYYTNVGRERCPVIDTWWQTETGGHMINPLPG
                     VTPMKPGSCSLPLPGVDAIVADEFGEEAERGKGGFLVVRRPWPGMIRTIWGDPERFKK
                     TYFPSEQKGHYLAGDAAQRDADGYFWIMGRIDDVLNVSGHRLGTMEVESALVANDLVA
                     EAAVVGRPDDTTGEAIVAFVVLKGALPEGDEAIAVAKQLRDWVSKEIGPIAKPRDIRF
                     GENLPKTRSGKIMRRLLRIVAKGETVTQDVSTLENPAILDQLARSV"
     gene            926..1828
                     /locus_tag="VIR04_01880"
     CDS             926..1828
                     /locus_tag="VIR04_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY9459530.1"
                     /translation="MKSGQLDHAALLLMGVTVLIWAFNWIVMKEMARYVGPFDLVFLR
                     FLFAFPLLFLMLLASRQKLKFPPFRLTVLIALFQMAAFACLSQFALIDGGAGHVVMLT
                     YTMPFWVVLLAWLLLGYRPTRRHVIGFVLAAFGLLAVIAPWQGLGTLRSSILAIGGGF
                     CWALGTVFSQMLFQRHRVNVLNLTAWQMFLGALFTLPFALVVPQQALEWRPEVIWGLA
                     YMAVMASAAGWGLWLLVVRRVSATIAGLSSLGVPVLAVILAWILLAERPTALELVGIV
                     LMMAGLVVVNWSSSAPGRVRRSGV"
     gene            complement(1842..>2830)
                     /locus_tag="VIR04_01885"
     CDS             complement(1842..>2830)
                     /locus_tag="VIR04_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY9459531.1"
                     /translation="RVDAAAVASCREAGAVVLGKTVTTEFATYTPGPTRNPHNPAHTP
                     GGSSSGSAAAVADYMTHLAFGTQTAGSIIRPAAFCGVVGYKPSFGHISRAGVKSLSES
                     LDTVGGFGRSVDDVALLAAAITRDTRLLNLDIDAAPRIGLCRTPHWASADEDAHAAIE
                     SAASALSAAGAHVSDASLPEVFSRLGDIQNQIMAFEAFQALSHERRVAYAKISPRLVE
                     LLELGAAISYENHQRNLVLAGSARRDIDALFDRYDALLTPGAPGEAPLFEMGTGNPLF
                     CRSWTLLGLPCVQLPFTSGTHGLPVGVQLIGRFATDYRLLQSARWAQEQLRR"
BASE COUNT          492 a          759 c          912 g          667 t
ORIGIN      
        1 gctcggtcgg cgagccaatc aatccggaag cctggatgtg gtattacacc aatgtgggtc
       61 gcgagcgctg cccggttatc gacacttggt ggcaaaccga aaccggcggg cacatgatca
      121 atcccttgcc gggtgtcacg ccgatgaagc cgggctcatg ctccttgccg ctgcctggcg
      181 ttgatgcgat cgttgccgac gaattcggtg aagaagccga gcgcggaaaa ggtggattcc
      241 tggtcgtcag acgcccgtgg cccgggatga ttcgcactat ttggggcgac cccgaacgct
      301 ttaaaaagac atattttcct tccgagcaaa aaggccatta tctggcgggc gatgccgcgc
      361 aacgcgatgc cgacggttat ttttggatta tgggccgcat cgacgatgtg cttaatgtgt
      421 caggtcaccg tttgggcacc atggaagtgg aatccgctct ggttgccaac gatctggtgg
      481 ccgaggccgc ggtggtcggt cgcccggatg acaccaccgg cgaagcgatc gtggcgtttg
      541 tggtcttgaa gggtgcactt ccagaagggg acgaagccat cgccgtcgcc aagcaacttc
      601 gcgactgggt ctccaaggaa atcggaccta tcgccaagcc ccgcgacatc cggtttggtg
      661 aaaatcttcc aaaaacgcgt tcagggaaga ttatgcggcg tttgcttcgt atcgtggcca
      721 agggcgaaac agtcactcaa gatgtgtcta cactagaaaa tccggcaatt ttggaccaat
      781 tggcgcgctc ggtgtaaggc gggcttgatg tttgaaactg actggcgttg ttcaggcgcc
      841 ggtcagcgat agtgcaagta atatgtaggg catggaaggc gatgtttgtt gccttccatg
      901 ccattttttc gtttaggagt cttttttgaa atctggccag ttggatcatg ctgcactgtt
      961 gttgatgggt gtcacggtgc tgatctgggc gttcaactgg atcgtcatga aagaaatggc
     1021 ccgttacgtc ggtccgttcg acttggtgtt tttgcgcttt ttgttcgcct tcccgctact
     1081 gtttctaatg ttgctggcat cgcgtcagaa gttgaagttt cctccttttc ggctgaccgt
     1141 attgatagcg ctgtttcaaa tggccgcttt cgcatgcctt tctcagttcg ccctgattga
     1201 cggaggcgcc ggccatgtag tgatgctgac ctatacgatg ccattttggg tggtgttgct
     1261 tgcgtggcta ttgctggggt atcgccccac ccggcgccac gtgatcgggt tcgtgctggc
     1321 ggcgttcggc ctgctcgccg tcattgctcc gtggcaaggc ttgggaacac tgcgtagttc
     1381 gatccttgca attggcggcg gattttgttg ggcgcttggc acggtttttt cgcaaatgct
     1441 gtttcagcgt catcgtgtca acgtgcttaa tttgacggcc tggcaaatgt ttcttggcgc
     1501 gttgttcact ttgccgtttg cgctcgttgt ccctcaacag gcactggaat ggcggcctga
     1561 agtgatctgg ggcctcgcct acatggccgt gatggcgtcg gcggcgggct ggggcttgtg
     1621 gttgcttgtc gtgcgccgtg tcagcgcgac gatcgcgggc ctgtcaagcc ttggggtccc
     1681 ggttctggct gtcattctgg cgtggatttt gctcgccgaa cgcccgaccg ccctggaact
     1741 ggtgggcatt gttttgatga tggccggtct ggtggtggtg aactggtctt catcggcgcc
     1801 tgggcgtgtg cggcggagcg gggtctgaca tcgcgaccgg actatcggcg caattgttct
     1861 tgcgcccagc gggcgctttg caataagcgg taatcggttg caaagcgccc gatcagttgc
     1921 acgcccacag gcaggccatg tgtaccgctt gtgaagggca attgcacgca gggcaacccc
     1981 aaaagcgtcc agcttcggca aaataaaggg ttgccggtgc ccatctcgaa aagcggtgct
     2041 tctccgggcg ctccgggcgt cagtagcgcg tcgtaacgat cgaacagagc atcaatatca
     2101 cggcgcgcgc tccctgccag gacaagatta cgctggtggt tttcgtacga tatggccgcg
     2161 cccaactcca gcagttccac cagcctgggg ctaattttcg cgtaggctac ccgccgctcg
     2221 tggctaagcg cctggaaagc ctcgaaggcc ataatttgat tttgtatatc gcccaggcgc
     2281 gaaaagactt cgggcagcga tgcgtcggac acgtgcgcac cggctgccga caaggcgctg
     2341 gccgcggatt caatggccgc gtgcgcgtcc tcgtccgccg atgcccagtg gggtgtgcgg
     2401 cacagcccta tgcgcggcgc ggcgtcaatg tccagattca gcaagcgtgt gtcgcgggtg
     2461 atggcggcgg ccaggagggc gacgtcgtcc acgctgcggc cgaatccgcc aacggtgtcc
     2521 agcgattcgc tgagactttt gacgcccgct cgcgagatgt ggccgaagct tggcttgtag
     2581 cccacgactc cgcaaaatgc ggcgggccgt atgatggatc ccgccgtctg cgtgccgaag
     2641 gcaagatggg tcatatagtc ggccacggcg gcggccgatc cgctggacga accacccggt
     2701 gtgtgagcgg gattgtgcgg gttgcgcgtg gggccgggtg tgtaggttgc aaattcggta
     2761 gtaacggttt tacccaggac tactgcgccg gcttcgcggc atgaggcgac tgcggcggca
     2821 tcgacacggg
//
LOCUS       DATCXU010000084         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_603244,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000084 DATCXU010000000
VERSION     DATCXU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603244"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VIR04_01890"
     CDS             complement(<1..558)
                     /locus_tag="VIR04_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY9459532.1"
                     /translation="MNPRLDTLQPYPFERLRLLLAQAGAPPAMLTPINLSIGEPKHAT
                     PPFIVKAMQNAMKGLSAYPPTKGDPALRGAIADWIANRYGIARPDPEIQVLPVLGSRE
                     ALFAFAQAVLDPNAHARVICPNPFYQIYEGAALLAGARPYYVNTRNAPGFNYDWASVP
                     AEIWRETQLLFVCSPDNPTGRVVSLD"
     gene            651..735
                     /locus_tag="VIR04_01895"
     tRNA            651..735
                     /locus_tag="VIR04_01895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:685..687,aa:Leu,seq:tag)
     gene            complement(760..1485)
                     /locus_tag="VIR04_01900"
     CDS             complement(760..1485)
                     /locus_tag="VIR04_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY9459533.1"
                     /translation="MHRKDNWLNIVSPLEIESTLRFILDSKTLLRMEAEGGSLSVITT
                     ILAIDADTIFVDDTTNTSAGSQFFKAGPVLLEAALKGMQISFDGAKIMASTHAGQPAL
                     RMAMPKLLRRLQRREFARIKVPVKSPALCTLPTPHQPQENIRLALRDIGPGGLSLIDG
                     QQLLGDAVGTHYKDCRLELPDVGWGNVGLRVVHNSDMPLKNGKTSRNVGCVFVDVSDT
                     TLMLLRHYAWRLEQMRINPQRPK"
     gene            complement(1535..>2161)
                     /locus_tag="VIR04_01905"
     CDS             complement(1535..>2161)
                     /locus_tag="VIR04_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY9459534.1"
                     /translation="ELPRTKFVCINLSGMSLNDEKFIDEFFVILNDFKHLSQMLCMEI
                     TESVALYDMKNTREFIRRLQALGVRTALDDFGAGYTSFPYLAELPADAIKIDGSFIKN
                     ISKHPVHSAIVEAIITLARNLGMKSIAEWVEDAPTLNMLREMGVDYVQGNIIAKPQHP
                     ARILEAADVGCLIIDQAVKASVCDALPAELLADSGTKRAPISTKPRQY"
BASE COUNT          467 a          549 c          621 g          524 t
ORIGIN      
        1 atccagcgac acgacccttc ccgtggggtt gtcgggcgag cagacaaata agagctgtgt
       61 ttcacgccat atctcggcgg ggacgctggc ccaatcgtag ttgaaaccag gcgcattgcg
      121 cgtattcaca taataggggc gcgcgcccgc aagcagtgcg gcgccctcgt atatctgata
      181 gaagggatta gggcagatga ctcgcgcatg cgcattaggg tccagcaccg cctgggcgaa
      241 cgcgaatagc gcttcgcgcg accccaaaac cggcagcact tgtatttccg ggtcgggccg
      301 cgcaatcccg tagcgattcg caatccaatc agctatggcg ccgcgtaggg ccggatcccc
      361 cttggtgggg gggtaagctg acaggccctt catggcgttt tgcattgcct tgacaatgaa
      421 aggcggggtg gcgtgtttgg gctcgccgat tgaaaggttg atgggagtca acatcgccgg
      481 gggcgcgccc gcttgggcca gcagcagccg aagccgttcg aaagggtaag gttgtaacgt
      541 gtcaagtctt ggattcatgg ccgcggcggg gaagatcggt ccacgatttt aacaagggaa
      601 tcggcttgta cataaaaaag attgcagcta aactggcggg cttaccattt gcgaacgtgg
      661 cggaattggt agacgcacca gatttaggtt ctggcgccgc aaggtgtgag agttcgagtc
      721 tctccgttcg caccacactc gggcttttat tttgggcctt tattttgggc gttggggatt
      781 tatgcgcatt tgctccaggc gccaggcgta atgccgcaat aacataaggg tggtatcgga
      841 tacgtctacg aagacgcagc caacattgcg actcgttttc ccgtttttta aaggcatgtc
      901 gctgttgtgt acgacgcgca gcccgacatt gccccaaccc acatcgggta attcgagccg
      961 gcagtcttta tagtgcgtgc cgaccgcatc accaagcaat tgctggccgt cgatcagaga
     1021 caggccgcca gggccgatat cccgtagcgc cagccgtatg ttttcctgcg gctgatgggg
     1081 cgttggcaag gtgcacagcg caggcgattt aacaggtact ttaatgcggg cgaactcgcg
     1141 tcgttgcagg cgtctgagca atttcggcat ggccatccgc agagccggtt gtcctgcatg
     1201 agtcgacgcc atgattttcg cgccatcgaa gctgatttgc attcctttca gggcggcttc
     1261 aagaaggact gggccagcct tgaaaaactg cgagccggca ctggtgttcg ttgtgtcatc
     1321 tacgaaaatt gtatcggcgt cgatcgccag gatagtcgtg ataaccgaca aagaaccgcc
     1381 ttctgcttcc attcgcagca atgttttact gtccaggata aaacgcagtg tggattcgat
     1441 ttcgagcggc gagacaatgt taagccagtt atctttccgg tgcatgggta gcggcccttt
     1501 ttgatccaac gtgatcggcg acagatggtg ctcgtcagta ctggcgaggc ttcgtggaaa
     1561 tgggcgcgcg ttttgtcccg gaatccgcca aaagctctgc gggcagtgcg tcgcacacac
     1621 ttgctttcac agcttggtcg attataaggc atcccacatc cgcggcctct agaatgcggg
     1681 ccggatgctg tggcttggcg ataatattgc cctgaacgta atcaactccc atttcccgca
     1741 acatgttcag ggtgggcgca tcttcgaccc attccgcgat gcttttcatt ccaaggttgc
     1801 gtgcaagcgt gatgattgct tcgacaattg cactgtgaac tggatgtttg ctgatatttt
     1861 taatgaatga tccgtcaatt tttatggcgt cggcaggcag ttcggcaagg tagggaaacg
     1921 aggtataacc ggcgccgaaa tcatccagcg cggtgcgtac acccaaagcc tgcaatcgcc
     1981 ggatgaattc ccgtgtgttt ttcatgtcgt acaaggcgac actttcggta atttccatgc
     2041 acagcatttg tgacaggtgt ttgaaatcat tcaatataac gaaaaattca tcgatgaatt
     2101 tttcatcgtt cagagacatt ccactaaggt tgatgcacac gaattttgtt cttggcaatt
     2161 c
//
LOCUS       DATCXU010000085         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_735801,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000085 DATCXU010000000
VERSION     DATCXU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735801"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VIR04_01910"
     CDS             complement(<1..552)
                     /locus_tag="VIR04_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9459535.1"
                     /translation="MNLQQFRFVRETIRRNFNLTEAARSLFTSQPGVSKAVIEFEDEL
                     GVKIFERHGKRIKGLTKPGILVAQVIDRIMREVDNLKKVSDEFARRDEGGLVIACTHT
                     QARYLLPKVIPAFRDRFPKVHLSLAEGNPPQLARMVLHEQADLGIATETLSLTPGLVA
                     LPVYSWQHAIVVRPDHPLAELTSA"
     gene            complement(626..2137)
                     /locus_tag="VIR04_01915"
     CDS             complement(626..2137)
                     /locus_tag="VIR04_01915"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY9459536.1"
                     /translation="MSAVPRTLDVPRIPLLIGGELVQSKTTDWRDVLNPATQEVVAQV
                     PFATKEELDRAVADSKQAFKTWRNASQATRVRVMLNLQRLVRENTAKLAELISLEHGK
                     TLPDAEGEVGRGLEAIEHACSMSTLQIGEYAENAARGIDVYTLVQPLGVCGGITAFNF
                     PIMLMCWMFPIAVTAGNTFVLKPSEQDPSSSLFLAQLALEAGLPPGVLNVVHGGPEIA
                     NALVDHPDIKAISFIGSSHVGTEIYRRASNLGKRCQAGMGAKNHCVILPDADRNAALN
                     QLIGAAFGAAGQRCMATSVAVFVGEAQKWVPDFVERAKKLKVNVGADRDADLGPLVSP
                     NARKRVEGLITQGVDEGAKLLLDGRGIKVSGYEKGNFVGPTVFGGVKADMAIYREEIF
                     GPVLDIVEVDTLEEAIEFINENPNGNGVGIFTQDGGAARFFQNNIDVGNVGINIPIPV
                     PVAWFSFTGSRASKWGDLGPNGKESIRFWTQNKTVTARWSAHNSSVNTTIAQH"
     gene            complement(2427..>2879)
                     /locus_tag="VIR04_01920"
     CDS             complement(2427..>2879)
                     /locus_tag="VIR04_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY9459537.1"
                     /translation="RTIDFDRYGFRLQSKKSASSMKAVREQAERQIKARPTTLLVGDD
                     NDPARAQLMWRIALPLAALNLALLAIPLGAVNPRLGRSADFLIAGLVGLLYMNLINLS
                     RGWIGSGQLGFGVGVWLVHGIFTALMVFMLWRRLRVKAPKKTHQLAPV"
BASE COUNT          685 a          804 c          755 g          635 t
ORIGIN      
        1 ggccgaggtt aattcagcca atgggtggtc gggacgcacg acaatagcgt gctgccacga
       61 ataaacgggc agagcaacca ggccgggagt cagcgacaac gtttctgtgg ctatccccaa
      121 atcggcctgc tcatgcagaa ccattctggc aagctgcggc ggattccctt cggccagcga
      181 caaatgcact ttcggaaacc tgtcgcgaaa agcaggaatg accttgggca aaagatagcg
      241 cgcctgcgta tgcgtgcaag cgatcaccag cccgccctcg tcgcggcggg caaattcgtc
      301 gcttaccttt ttcaagttat cgacttcgcg catgatccgg tcaataacct gtgccaccag
      361 aatgcccggc ttggtcaaac cttttatgcg ttttccatga cgttcgaaga ttttgacccc
      421 gagttcatct tcgaattcga tgacagcttt ggagacgccg ggctgggatg tgaataagga
      481 acgcgccgcc tcggttaaat taaagttgcg ccgaatagtt tcgcgtacaa aacgaaattg
      541 ctgaagattc ataaaaaaat agccctttag gaagggctat tatatgtaat aaagaaataa
      601 acgtatatta tttctagtta tatgcttaat gttgcgctat tgttgtattc acagatgagt
      661 tgtgagcgga ccagcgtgcc gtgacggttt tgttctgcgt ccagaatcga atggactctt
      721 tgccgttagg acccaggtcg ccccatttgg acgcacgcga gccagtaaag ctgaaccagg
      781 ccacgggcac cggaattgga atattgatgc cgacgttgcc cacgtcgata ttgttctgga
      841 aaaagcgtgc cgcgccacca tcctgggtga aaataccgac tccgttgccg ttcggattct
      901 cgttgatgaa ttcgatggct tcttccagcg tatcgacttc tacgatgtcg agcacgggcc
      961 cgaaaatttc ttcgcgatag atggccatat cggctttaac gccgccaaag acagtcggtc
     1021 ccacgaaatt acccttttcg tagcccgaca ccttgatgcc gcgcccatca agaagcaact
     1081 tggccccttc gtcgacgccc tgcgtgatta gcccttctac ccgcttgcgc gcgttaggcg
     1141 agacgagagg accgagatcc gcgtcgcgat ccgcaccgac gttaactttg agcttcttgg
     1201 cgcgttcaac aaagtcggga acccattttt gcgcctcgcc cacaaacacc gccaccgagg
     1261 tggccatgca acgctggccg gcggcgccaa acgcggcccc tatcaactga ttgagtgccg
     1321 cattgcgatc ggcgtcgggc aaaatcacgc agtgattctt ggcgcccata ccggcctggc
     1381 aacgcttgcc aagattgctg gcacgacggt agatttcggt gcccacatgg gacgatccaa
     1441 taaacgaaat agctttgatg tcgggatggt cgaccagcgc gttggcgatt tcggggccgc
     1501 catgaacgac gttcagcaca cccggaggca gaccggcttc aagagccaat tgggctaaaa
     1561 acaaggacga gctgggatcc tgttcggacg gcttcagcac gaaggtattg cccgcggtaa
     1621 ccgctatagg aaacatccag cacatcagca tgatggggaa gttgaacgct gtgatgccgc
     1681 cgcaaactcc gagcggttga accagcgtgt agacatcgat gccacgagct gcattttcgg
     1741 catattcacc aatttgcaga gtactcatgg agcacgcatg ctcgatcgct tccagcccgc
     1801 gccctacttc gccttcggca tcgggcaggg ttttgccgtg ctccagactg atcagttcag
     1861 ccagtttggc ggtgttttcg cgaaccagtc gttgcaaatt cagcataacg cgcacacgcg
     1921 tggcctgact ggcgttgcgc catgtcttga aggcttgctt cgaatctgcc actgcgcgat
     1981 ccaattcttc tttagtcgca aatggcacct gtgcaaccac ctcctgagtt gcgggattaa
     2041 gaacatcgcg ccaatcagtg gttttagact gcacaagctc gccaccgata agcaaaggaa
     2101 tacgggggac atccagggta cggggaacgg cactcattaa aatctccttg gcgcgtacaa
     2161 tgcgcaaatg taaatcggtt tactttttaa ctaaagcgca ggtcgactca gacaaaggct
     2221 gaaaggcttc ctgcgccgga attgtggtct gaagtttgaa cagatcccgc aggccccgtt
     2281 ttgagccggg ttcccgcatt ttcgccgcca agacatcgcc atgagtccag cccaaggcga
     2341 gcgcctgcaa gcagtgcgat aagctcgaat atactcccgc cggcgttatt gtgggtttca
     2401 aatttttgaa acgtattttc atcacattaa acaggcgcaa gttggtgtgt ctttttcgga
     2461 gccttcaccc gcaatcgacg ccacagcatg aacaccatca aagcggtgaa tatcccgtga
     2521 accagccaca cgccaacacc aaaaccaagt tggccactgc caatccagcc gcgcgacaaa
     2581 ttaattaaat tcatatagag caaacccact aggccggcta tcagaaagtc ggctgaacgc
     2641 cccaatcgag ggttcaccgc tcccagcgga atggccagca aggccaggtt caatgccgct
     2701 agcggcaagg caatgcgcca catcagttgc gcacgcgccg gatcgttgtc gtcgccaacc
     2761 aaaagcgtgg tgggccgagc cttgatctgc cgctcggcct gctcgcgcac tgccttcatt
     2821 gagctggcgc tttttttgct ttgaaggcga aaaccgtaac gatcaaaatc aatggtgcg
//
LOCUS       DATCXU010000086         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_150108,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000086 DATCXU010000000
VERSION     DATCXU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150108"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2426
                     /locus_tag="VIR04_01925"
     CDS             <1..>2426
                     /locus_tag="VIR04_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEY9459538.1"
                     /translation="TSQLSIALVNWLITLATRPHYLPRMDFSTGIPPESSTLVVVPTM
                     LLTEQNIESLCDALEVRFLANRDQYLRFCLLTDFSDAASETTDTDDALLRQAQQGIRA
                     LNAKYAGEPSEESPDDKFLLLHRPRRWNAQEKIWMGYERKRGKLGDLNAYLRDPTNNP
                     FSCIVGDTTGLSTVKYVITLDTDTRLERDSARQFVGAMSHPLNRAVYDEGQRRVTQGY
                     GILQPRVSTSLPARNASHYGLLFGGEPGIDPYTRTVSDVYQDVFGEGSFVGKGIYDVD
                     AFEQALANRFPENHILSHDLLEGCYARCGLISDVQLHEEYPVRYSADVKRRHRWIRGD
                     WQLAAWLFPRVPVAAIAQSNKRWEKNPLSTLSRWKLLDNLRRSVVPTALTSLFLLGWI
                     ILPSGWLWTTTVLSILFLPPLLASLIDLLRKPENVLLRQHIIATARYARQRFAQALLS
                     LTFLPYETFYSLDAVVRTLWRQLISHKNLLEWNPSHAEHADRTTLDASYRRMWASPFI
                     AIASIIVLAATSPHALWSTAAPILVLWLLAPAIAWWISQPLPRDEVRLTGEQTLFLHR
                     LSRKIWAFFENYVGPDDNWLPPDNVQIEPISLVAHRTSPTNIGLALLATLAAHDFGYI
                     TGGQLIERCANTLRTMDALERHQGHFYNWYDTQHLNPLLPMYISTVDSGNLAGHLMTL
                     RPGLLALLDQPIIARRLFDGIHDTLDVLTERVSDAARGKLNEFRIYLADACKSPPATI
                     SAALQCLHQLAQLAGTLADHPELAEHTDPITNRWVQALNRQCTDAYNDLKHLAPWGDT
                     LHSPDWDAETL"
BASE COUNT          516 a          742 c          657 g          511 t
ORIGIN      
        1 acaagccagt tgtccatcgc gctggtgaac tggctgataa ccctggccac ccggccgcat
       61 tatctgccgc gcatggattt ttccacaggg ataccacctg aatcaagcac cctggtcgtc
      121 gtgccgacga tgctgctgac agaacaaaat attgaatcgt tatgcgatgc gctggaagtc
      181 cgttttctgg ccaatcgcga ccaatacctg cgcttctgcc tgcttacgga tttttccgat
      241 gcggccagcg aaacgacaga cacggacgat gccttgctgc ggcaggcgca acaaggcatc
      301 cgtgcgttaa acgcaaaata tgccggtgaa ccttccgagg aaagcccgga cgacaaattc
      361 cttttgctgc atcggccgcg ccggtggaat gcgcaggaaa aaatctggat gggctacgag
      421 cgcaaacggg gcaagctggg cgatctgaac gcgtacttgc gcgacccgac caacaatcct
      481 ttctcgtgca tcgtgggcga caccaccggt ctttcgacag tgaaatacgt gatcacactc
      541 gataccgaca cccggctgga acgcgactcg gcgcggcagt tcgtaggcgc catgtcgcac
      601 ccgcttaatc gggccgtgta cgacgaaggg cagcggcgcg tcacgcaggg gtacggtatc
      661 ttgcagccgc gtgtttcgac cagtctgccg gcgcgaaatg cctcgcatta cggactgctt
      721 tttggcggcg aacccggcat cgatccctat accagaaccg tgtcggatgt gtaccaggac
      781 gtattcggcg aaggctcttt tgtggggaag ggcatctacg atgtcgatgc cttcgagcaa
      841 gcgctggcaa accgttttcc ggaaaatcat attctcagcc acgatttgct ggaagggtgc
      901 tacgcccgtt gcggtctgat ctccgacgtg caattgcacg aggaataccc tgtccgatac
      961 agcgcggacg tcaagcgccg gcaccgctgg atacggggcg actggcaatt ggcggcgtgg
     1021 ctattcccgc gcgtgccagt cgcggcgatc gcccaatcaa acaaacgctg ggaaaaaaat
     1081 cccttatcaa cgctctctcg ctggaagttg cttgataatc tgcgtcgcag cgtagttccc
     1141 accgcactga cctcgctatt cctgttgggc tggatcatat tgccgtccgg gtggctgtgg
     1201 actacgacgg ttctcagcat acttttcctg ccacccttgc ttgccagcct gatcgacttg
     1261 ttgcgcaagc ccgaaaatgt tttgctgcgg caacacatca tcgccacggc gcgctatgcg
     1321 cgacagcgat ttgcacaagc attgttgtcg ctgactttcc ttccctatga aactttctac
     1381 agcctggatg cggttgtccg cacattgtgg cgccaactga tcagccataa aaatctgctg
     1441 gaatggaacc cgagtcatgc cgaacatgcc gacaggacca ctctggatgc gtcgtaccgc
     1501 cgcatgtggg ccagcccatt catcgccatt gcaagcatca tcgttctggc cgcgacaagt
     1561 cctcacgcgc tatggtcaac cgcggcgccg atcctggtgc tgtggcttct tgcaccggcg
     1621 atcgcatggt ggatcagcca gccgcttcca cgcgacgaag tgcgcctgac cggcgaacaa
     1681 actttatttc tccatcgcct gtcccgaaag atatgggcct ttttcgagaa ttacgtcggg
     1741 ccggacgata actggctgcc accggacaat gttcagatcg aacccatatc gctcgtagcc
     1801 catcgcacct cgcccaccaa tatcgggctg gcgctgctgg ccactttggc cgcccatgat
     1861 ttcggctaca ttaccggggg ccagttgatc gaacgctgcg ccaacacgct gcgcaccatg
     1921 gacgcactgg aacggcatca gggtcacttt tacaactggt acgacaccca acatttgaac
     1981 cctttgctgc cgatgtatat ttcgaccgtc gacagcggca atctggctgg ccacctgatg
     2041 acattgcggc ccggcttgct ggcattgctg gaccagccca tcattgcgcg ccggctattc
     2101 gacggcatcc acgacaccct tgatgtactg acggaacgcg tttccgacgc agcgcgcggg
     2161 aaattgaatg aatttcgcat ttacctggcc gacgcatgca aatcgccgcc cgcaacaatc
     2221 agcgcggcgc tgcaatgtct gcatcaattg gcgcaacttg cgggaaccct tgccgatcac
     2281 cccgaactcg ccgaacacac ggaccccata acaaaccggt gggtacaggc gctaaatcgt
     2341 caatgtacgg acgcgtacaa cgatctgaaa catcttgctc cgtggggcga cacgctgcat
     2401 tcgccggatt gggatgccga aacact
//
LOCUS       DATCXU010000087         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_183457,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000087 DATCXU010000000
VERSION     DATCXU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183457"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VIR04_01930"
     CDS             <1..331
                     /locus_tag="VIR04_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY9459539.1"
                     /translation="AMGEQLVSELSAKWEPRHFTDSFKEQILRLVEEKIATGETESVT
                     QPDAKEQDAPGAKIYDLTELLRQSLNKGTRTPAKKSPASKKAARPKKKTTVAAKKPGS
                     HSGRKTA"
     gene            335..2806
                     /gene="ligD"
                     /locus_tag="VIR04_01935"
     CDS             335..2806
                     /gene="ligD"
                     /locus_tag="VIR04_01935"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY9459540.1"
                     /translation="MTKADPLARYKSRRDFSITAEPSGGGLANESARSFVVQKHWASR
                     LHYDFRLELDGTMKSWAIPKGPSFDPRDKRMAVHVEDHPISYNTFEGQIPAKQYGAGK
                     VIVWDKGTWAPLGDPRKDYRAGKLKFELVGHKLRGRWTLVRMKGKQAKQDPWLLIKEK
                     DEFSRSVTEFNVVDEMPDSVARLGNSSQPAVDANGTAPSAGVRAKLPSAMKPQLATLV
                     ETPPIDSDRWLYEIKFDGYRLLARIHKRTVHLFTRSGNDWTGKLATLASELARAKLPD
                     GWYDGEIVVPDEHGVPDFQALQGAFDNSKTQNIVYYLFDLPYCDGMDLRAAPLEQRRR
                     KLQEMLLKIGGSSIRFSETFNAGGRDIVASACKLGLEGIIAKRKTSHYVSRRSPDWIK
                     LKCGLRQEFAIGGFTDPKGSRAGIGSLLLGVHDQSGALVYAGNVGTGFNARTLGDIRQ
                     KLNALQTDRNPFSTSTGIDKKAHWVRPSLLAEISFGQWTNTGRVRHAVFHGLRTDKPA
                     KGIIREIAMPSIKSDAPHSLLAKFKVTHPDRIVDALTKTSKIDVIRHYALVAPLMIEH
                     LKGRPVSLVRAPEGVKGEMFFQKHMETAKMAGIRQLPQVLDPGHEPLMEIASAQGLLS
                     AAQMNVIEFHTWNAVKAPIGKPDRMTFDLDPGKGIEWPAVQEAARLVHAFLDELQLAC
                     FLKTSGGNGLHVVVPLRRRHDWDTVKDFSQAIVLHLAKTLPNRFTATSGPRNRVGKIF
                     IDYLRNGFGATTVAAWSARARPGMGVSVPVAWNEIDQLRSSAHWHIGNVHERLDKGNE
                     PWRDYGKSANPLGGAMKIMGFKPRK"
     gene            2930..3169
                     /locus_tag="VIR04_01940"
     CDS             2930..3169
                     /locus_tag="VIR04_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459541.1"
                     /translation="MKLLDKLKQGSTPSRFSTNSARAPIDRREQRKLDQAQGLVSFPL
                     KLKQPVIEEIRTRAAAQNTGTSEIVEALLEKALKL"
     gene            3230..4177
                     /locus_tag="VIR04_01945"
     CDS             3230..4177
                     /locus_tag="VIR04_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY9459542.1"
                     /translation="MKYVDYYQALGVSRDATQEDIKKAYRKLAHKYHPDVSKTADAEE
                     KFKTIAQAYATLKDPEKRAAYDTLGSHPQGEEFVPPRQWQEQFNEAPGAFADVDLADL
                     LAAFAAAQRGNAREHAQRPLRGQDFEVPVSVTLEQIYAGAETEISVALPEYDQQGLLH
                     RIPKTFSVRLPKGASSGQRLRLAGKGGSGLNGGKPGDLYIVMEIQPHKLYRVSDNDLY
                     MDLPLAPWEAALGANVQIPTLGGSVEMTIPSGTIAGRKLRLSKRGLPSANGVHGDLYA
                     VVNIQVPRALTARERELFTQLAAESKFNPRAQLNAGARP"
     gene            4174..4509
                     /locus_tag="VIR04_01950"
     CDS             4174..4509
                     /locus_tag="VIR04_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEY9459543.1"
                     /translation="MTTVHITESIWLNASDICSFDHLLETSGLTRSDLLDLVEAGIIE
                     PSNQDPDDYLFSSNCIVIARTARRLRDDFELESRGLALALNLIRRADRLEAELAELRA
                     RFLQEKKTT"
     gene            complement(4518..4814)
                     /locus_tag="VIR04_01955"
     CDS             complement(4518..4814)
                     /locus_tag="VIR04_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY9459544.1"
                     /translation="MKTLPSNHNVETITARITGHVQGVGFRAAAVRQAHLVGVTGWVR
                     NARDTSVEVLLQGTHDQIDLMLSWLHHGPSAARVDSVSSEREFTDRHFDRFEQQ"
     gene            complement(4839..>5260)
                     /locus_tag="VIR04_01960"
     CDS             complement(4839..>5260)
                     /locus_tag="VIR04_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459545.1"
                     /translation="PSLLELRSLLDVLAFHESTDAYLDDQAIYWIDDADPDSVCVVRI
                     RDKSQSHKKPYELPGWRLLHSVPASRANTNIIDIYKAPKRGMAVSKAKALPIIVYRGD
                     AYKVWLADRVAASNSEHHDWGVHRLSPSPESAWLANA"
BASE COUNT         1274 a         1446 c         1499 g         1041 t
ORIGIN      
        1 ggccatggga gagcaactgg tctcggagtt gagcgcgaag tgggagccgc gccattttac
       61 agactcgttc aaggaacaaa tattgcgcct ggtcgaagag aaaattgcta ccggcgaaac
      121 cgagtccgta acgcaacccg acgccaaaga acaggacgcg ccaggcgcaa aaatctatga
      181 tctgaccgag ttgctgcggc aaagcctgaa taagggcacg aggacgccag cgaagaaatc
      241 gccggccagc aaaaaggcgg ctcggccgaa gaaaaagacc actgttgccg ctaaaaaacc
      301 tggcagccat tccgggcgca aaacggctta ggccatgacc aaggccgacc cccttgcacg
      361 ctacaaatcc aggcgtgatt tttcaataac cgccgaacct tccggaggag gactggccaa
      421 tgaatcagcg cgctctttcg tcgtccaaaa gcattgggca agccggcttc attacgactt
      481 tcgactggag ctggatggaa caatgaaaag ctgggccata ccgaaaggcc ccagcttcga
      541 tccgcgcgac aaacgaatgg ccgttcacgt ggaagaccac cctatttcat acaacacatt
      601 cgaaggacag attcctgcga aacagtatgg tgcgggcaag gtcattgtct gggacaaggg
      661 aacgtgggca ccgctgggcg atccccgcaa ggactatcgc gctggcaaat tgaagttcga
      721 actggtcggt cacaaactgc gcggccggtg gaccctggtc cgcatgaagg gaaagcaggc
      781 gaagcaggac ccttggctat tgattaaaga aaaggacgag ttttcgcgct ccgtgacgga
      841 attcaacgtg gtggacgaaa tgccggacag cgtggccaga ctaggcaata gttcgcaacc
      901 ggcggttgac gcgaacggga ccgcgccttc ggccggtgta agagccaaac ttccctctgc
      961 catgaaacca caactcgcca ccctggtgga gacgccgccc attgattctg accgatggct
     1021 ctatgagatc aaattcgacg gctatcgttt attggcacgg atacacaaac ggacggtcca
     1081 cctttttacg cgcagtggca acgactggac cggcaaactc gcaacactgg cctcggagct
     1141 ggcccgcgca aaattgcccg acggatggta tgacggcgaa atcgttgtgc ctgatgaaca
     1201 cggagtgccc gattttcagg cacttcaagg cgcattcgat aactcaaaga ctcagaatat
     1261 tgtttattac ctgttcgacc ttccctactg cgatggcatg gacctgcgcg ctgcgccgct
     1321 cgagcaacgc cggcgaaaac tgcaggagat gttgttgaaa atcggcggca gttccattcg
     1381 ctttagcgaa acgttcaatg ccggcggacg cgacatcgtg gcctcggcat gcaagctggg
     1441 actcgagggc attattgcca aaaggaagac ttcgcactat gtgtcgcgcc ggtcgcccga
     1501 ctggatcaag ctgaaatgcg gcctgcgcca ggaatttgca atcggcggat tcaccgatcc
     1561 caaagggtcg cgcgcaggta ttggctcgct gttgctgggt gtgcacgacc aatccggcgc
     1621 gttggtgtac gcaggcaatg tcggcacggg tttcaacgcg cgcacgctag gcgacatccg
     1681 gcaaaaactg aacgcattgc aaacggacag gaaccccttt tcgacatcga ccgggatcga
     1741 caaaaaagcg cactgggtca ggcccagttt gcttgctgaa atctcatttg gccaatggac
     1801 caacacgggc cgcgttcgcc atgcggtctt ccatgggctg cgcaccgaca agcctgccaa
     1861 ggggattatt cgagagatcg ccatgccttc catcaagtcc gacgcgcctc attcgctgct
     1921 cgcgaagttt aaagtgaccc atccagaccg gatcgtggac gcgttgacca agacatccaa
     1981 gatcgatgtc attcgtcact acgccttggt cgcccctctg atgatcgagc acctgaaggg
     2041 ccggccggta tcgctggtgc gagcgcctga gggcgtgaaa ggtgaaatgt ttttccagaa
     2101 acacatggag acggcgaaga tggccggtat acggcaattg ccgcaggtgc tggatccggg
     2161 ccatgagccc ctgatggaaa tagcgtcggc tcaaggcttg ctaagcgcgg cccaaatgaa
     2221 cgttattgaa tttcatacat ggaacgcagt gaaagcgcca attggcaagc cggatcgcat
     2281 gaccttcgac ctcgacccgg gcaaaggaat tgaatggccc gctgttcaag aggcggcgcg
     2341 tctcgtgcat gcctttcttg acgagctgca acttgcctgt tttttgaaaa ccagcggtgg
     2401 caacggtttg catgtggttg ttccgttaag gcgtcggcac gattgggata cggtcaagga
     2461 cttttcgcag gcgattgtgt tgcatctggc caagaccttg cccaatcgct tcacggccac
     2521 tagcgggccg cgcaaccggg tgggaaaaat ttttattgat tatttgcgta atggcttcgg
     2581 tgcgacgacg gtcgccgcat ggtccgctcg cgcgcggcca ggcatgggcg tatcggtgcc
     2641 cgttgcctgg aatgaaatcg atcaactgcg cagttcggcc cactggcata tcggcaatgt
     2701 gcatgagcgc ctggataagg gcaacgagcc ttggcgggac tatggcaaat cggccaaccc
     2761 tttagggggt gcgatgaaaa ttatgggctt caaaccacga aaataaccgt cgtgcgataa
     2821 ccagtccaac agggtcttgc gagtagaata gttcgaatca tgcttccatg gccaaaattt
     2881 ggattgaacg aaaactatga aaagaacaga cttggaaaag aacaaagcag tgaaattgtt
     2941 ggacaaatta aagcaaggca gcacaccaag ccggttctcg acgaattcgg cgcgcgcccc
     3001 catcgaccgg cgtgaacaac gcaagcttga tcaagcgcaa ggcctggttt cgtttccgct
     3061 gaaactgaag cagcccgtca ttgaagagat tcgcacgcgc gccgcagcgc aaaataccgg
     3121 caccagcgaa attgtcgaag ccttgctcga aaaggctctg aagctgtaaa ttgcgttacg
     3181 cccgcgcgca tgccgcggcg ttttgcaaag gttcaaaacg ggaatttcaa tgaaatacgt
     3241 tgattactac caggccttgg gcgtttcgcg cgacgcgacg caagaagata taaaaaaggc
     3301 ttaccggaag ctggctcata aatatcatcc tgatgtatcc aaaactgcgg atgcggagga
     3361 aaaattcaag acaatcgcgc aagcttacgc caccttgaaa gatcccgaga aacgagcggc
     3421 ttatgacacg ttgggcagcc accctcaagg cgaggaattc gtcccgccgc gtcagtggca
     3481 ggagcagttc aatgaagcgc caggcgcctt tgccgacgta gaccttgcgg atttgctggc
     3541 ggcattcgcc gccgcgcaac gcggaaatgc gcgggaacat gcgcaacgcc ccttgcgtgg
     3601 gcaggatttt gaagtccccg tatccgtgac cctcgaacaa atttacgccg gagcggaaac
     3661 cgaaatcagc gtcgctctgc cggaatacga tcaacaaggg ttgctgcacc gaatcccgaa
     3721 aacgttcagc gtccgtctgc ccaagggcgc ttccagcggc caacgcttgc gcctcgccgg
     3781 caaaggaggc tcgggcctga atggcggaaa gccgggcgac ttgtatatcg tcatggaaat
     3841 tcaaccgcat aaactgtatc gggtgagcga caacgacctt tatatggatc tgccgctcgc
     3901 cccctgggag gcggccctgg gagccaatgt gcaaataccc accttgggcg gttccgtcga
     3961 aatgacgatt ccatcgggaa caatcgccgg gcgaaagttg cgcctgtcca aaaggggatt
     4021 gccgtcggcc aatggtgttc acggcgacct gtatgcggta gtgaacattc aggtgccgcg
     4081 cgcattgacc gcccgcgagc gggaactgtt tactcaactc gccgccgagt ccaaattcaa
     4141 tccgcgtgct caacttaacg caggagcccg accatgacga cggttcacat cactgaaagc
     4201 atatggctta atgcgtcgga tatttgctcg ttcgaccacc ttctggaaac ctccggtctt
     4261 acgcgaagcg acttgctcga tttggtggag gccggcatta tcgagccatc aaaccaggac
     4321 cccgacgatt atctgttttc cagcaattgc atcgtcattg ccagaacagc gcgccggcta
     4381 cgcgacgact tcgagcttga atcgcgcggg ctggccctgg ccctgaatct gatcaggcgc
     4441 gcggatcggc tcgaagccga actggctgaa cttcgcgcgc ggtttctcca ggaaaaaaag
     4501 actacgtaat ggtgatgcta ctgttgttcg aaacgatcga aatgccggtc ggtaaattcg
     4561 cgttcactgc ttactgagtc aactctggcc gcgctcggtc cgtggtgcag ccaagacagc
     4621 ataaggtcaa tctgatcgtg cgtgccctgc agcagaacct cgaccgacgt gtcgcgtgcg
     4681 ttgcggaccc agccggttac gcccaccaag tgggcttgtc gcactgcagc ggcacggaaa
     4741 cccacaccct gcacatggcc ggtaattcga gcggtaatgg tttcgacgtt gtggttggac
     4801 ggcagagttt tcatgtggtt aaagactaaa ttaaaccgtc aggcatttgc cagccaggcc
     4861 gattcgggtg atggcgaaag acgatggaca ccccagtcat gatgttcgct attggacgcg
     4921 gcaacccgat cggcaagcca gaccttgtag gcgtcgccac ggtaaacaat gatcggcaac
     4981 gccttggctt tggacaccgc catcccacgc ttgggcgcct tgtagatgtc gattatattg
     5041 gtatttgcgc gcgatgccgg aacgctgtgc aaaagtcgcc agcccggtaa ttcgtacggt
     5101 tttttgtgtg actggctttt atcgcgaatg cgcacgacgc atacggaatc cggatcggca
     5161 tcgtcgatcc aataaatcgc ctgatcgtcc agataagcat cggtggattc atgaaacgcc
     5221 agcacatcca gcaaagaacg cagctccagc aagcttggca
//
LOCUS       DATCXU010000088         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1113663,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000088 DATCXU010000000
VERSION     DATCXU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113663"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VIR04_01965"
     CDS             complement(<1..764)
                     /locus_tag="VIR04_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459546.1"
                     /translation="MIKVWRRLRALFVWSWTLSGLLIIFGGSFVYWCVGTEPGARWTL
                     ETVAGQFDGHALGIEGSIWSGVRVQDLLIKQPEFSAHFENVQARIDWSSLSQHRLQIK
                     DLSAQGLDIDLTKPAPPAASPAPFKTPAVPLHIVLDRLAVGKLTLRDGGELVPLNVEN
                     LAASLDLSERGARLVLASIDLDHEAMRFSTRGDIELLALSDPWPLRARLVTQASGQSP
                     DSPLCVRHFVPSLPVVDQVKAPECSVEVESAIDGSL"
     gene            complement(761..>2081)
                     /locus_tag="VIR04_01970"
     CDS             complement(761..>2081)
                     /locus_tag="VIR04_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY9459547.1"
                     /translation="GKENLIDGVRGEDFFLARVVESQATVTAETAKADLRVAIDSGPR
                     VRLGEMNATGLKRVPPELIAHYIRYAPGDPYNQAQMDQWQQDLQSTSFFRGAFVSLDS
                     DPKHRTYLPNGDVQMPLMVRVSEAPAWRFSSSLGVDSDNGVRVESLYRQNIVWGQPVW
                     IETGIGVDKNRQRAFYDVHFVPTPSGYKDSVGALYNHSDIQGLDNTRVGIGWKRKQER
                     KAAGASRVEYETQWALIAAYDKTKIEGAVGFEVPTLVGTWQWLRRDVDNKYDPRQGNL
                     IDVGLGAGVTLDKREPFYRSSLRAQHWWPVGKRDTFTVRAEVGKVWSQTDRLPEDFGY
                     RTGGARTIRGYAFQSIGLPRGNAVVGAPALAVASVEYMHYFNEHFGMDVFVDAGDAAA
                     SFGQMKLAVGYGVGALVRTPAGPFSIDLAYGQRDHNVRVHFSLGIAF"
BASE COUNT          472 a          668 c          544 g          397 t
ORIGIN      
        1 tcaagcgagc cgtcaatagc cgattcgacc tccacagaac actctggcgc cttcacttgg
       61 tcaacgacgg gcagagaagg aacaaaatgg cgcacgcaca atggcgaatc gggactctgg
      121 ccgctcgcct gagtgacaag gcgggcgcgc aacggccacg gatcactaag cgccagcaac
      181 tcaatgtcgc cccgcgtcga aaaccgcatt gcctcgtggt ccaggtctat gcttgccagc
      241 acaagtcgcg cgccgcgttc cgacaaatcc agcgatgccg caagattctc cacgttcagc
      301 ggcacaagct cgccgccgtc gcgcaaggtc aacttgccca ccgccaaccg gtccagcacg
      361 atatgcaacg gcaccgccgg cgtcttgaaa ggagccggcg atgcggcggg cggcgccggt
      421 ttggtcagat cgatatccag cccctgtgcc gacagatcct tgatctgcaa gcggtgttgc
      481 gacaaggagc tccagtcaat ccgcgcctga acgttttcaa aatgggcgct gaattcgggt
      541 tgtttgataa gcagatcctg cacacgaact cccgaccata tcgagccttc tatacccagg
      601 gcgtggccgt cgaattggcc tgcaacggtt tccagagtcc agcgcgcccc gggctcggtg
      661 cccacgcacc agtaaacaaa agagcctcca aagataatca gcagacccga gagagtccag
      721 gaccagacga acaaagccct gagtcgacgc catactttga tcaaaatgca atacccaatg
      781 agaaatgaac acgcacattg tgatcgcgct ggccgtaggc gagatcaatg gaaaatgggc
      841 cggcgggcgt acggaccaat gcgcccacgc catagccgac agccagtttc atctgcccga
      901 acgatgccgc cgcgtcgccg gcgtcgacaa aaacatccat tccaaaatgc tcattgaaat
      961 aatgcatgta ttccacactg gccaccgcca gcgccggagc gcccactact gcgttgccgc
     1021 gcggcaggcc aatactttga aatgcataac cacgtattgt ccgggcgccg cctgtacggt
     1081 aaccgaaatc ctcgggcaga cgatccgttt gggaccacac cttgcccacc tcggcgcgca
     1141 ctgtaaacgt gtcccgcttg ccaactggcc accagtgctg agcccgcaag ctggatcgat
     1201 aaaacggttc gcgcttgtcc agcgtgaccc ccgcccccaa accaacgtcg atcaagtttc
     1261 cctgacgagg gtcatatttg ttgtctacgt cgcgccgcag ccactgccag gttccaacca
     1321 atgtgggcac ctcgaatccg acagcgcctt caattttcgt tttgtcgtag gccgcaatca
     1381 aggcccactg ggtttcgtat tcaacccggc tggcgcccgc cgccttgcgc tcttgtttgc
     1441 gcttccatcc gattcccaca cgagtgttgt ccaaaccctg aatatccgaa tgattgtaaa
     1501 gagcgccgac gctgtccttg tagccgctgg gagtcggcac gaaatgcaca tcatagaacg
     1561 cccgctggcg atttttatcg accccaatgc cggtttcgat ccagactggc tggccccaca
     1621 caatattctg acgatacagc gattccactc gcacgccgtt gtcgctatcg acaccgagcg
     1681 aactcgaaaa tctccatgcc ggggcctctg atacgcgcac cattaaaggc atttgcacat
     1741 cgccattggg caaataggtg cgatgcttcg gatcgctgtc caaagacaca aaggcgccgc
     1801 gaaagaacga cgtcgattgc aagtcctgtt gccattgatc catttgcgcc tgattgtacg
     1861 gatcgccggg cgcgtagcga atatagtggg caatcagctc gggcggaacg cgcttcaatc
     1921 ccgtcgcatt catttctccc aggcgcaccc ggggtccgct gtcgatggcc acccgcagat
     1981 cggctttggc agtctcggcg gtgacggtgg cttgcgattc aaccactcgg gccaaaaaga
     2041 aatcttcgcc gcgcacaccg tcaatcaaat tctccttgcc g
//
LOCUS       DATCXU010000089         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_106392,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000089 DATCXU010000000
VERSION     DATCXU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106392"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1340
                     /gene="argE"
                     /locus_tag="VIR04_01975"
     CDS             192..1340
                     /gene="argE"
                     /locus_tag="VIR04_01975"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY9459548.1"
                     /translation="MKRQSLDMITALVGFDTVSRNSNLPLIHYVKDYLSQQGVQSHVI
                     PSPDGNKANLFATIGPNAPGGIVLSGHTDVVPVDGQLWDTDPFQVTQKGDRLYGRGTC
                     DMKAFTAIALAMAPDMLKAKLKRPVHLALSYDEEVGCVGAPALIEHLSGHIARPEGVI
                     VGEPTSMRPIVAHKGITVLHTTITGHEAHSSQVQRGVSAVTTGAKLITFIDAMMAENK
                     AAADPECRFTPAYTTLHVGVVKGGTAVNIISRQCSFTWDIRTLPGDSWRRYLDRFQAY
                     ADELLPAMRAVSPDAAISTALIAQAPPLQDQGGGAQDLVYRALGHSNGDVVPYAAEAG
                     QYQERGFSVVLCGPGSIDQAHQPNEYIEISQVQACEAFFQTLIKQLAQ"
     gene            1488..1736
                     /locus_tag="VIR04_01980"
     CDS             1488..1736
                     /locus_tag="VIR04_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY9459549.1"
                     /translation="MGIISMIIVGLIIGLIARAIMPGDQKMGIILTIILGILGSVVAG
                     YLGGAIGWYAPGEGAGWIGSIVGAIIVLFIYGLIARKR"
     gene            1742..2116
                     /locus_tag="VIR04_01985"
     CDS             1742..2116
                     /locus_tag="VIR04_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY9459550.1"
                     /translation="MDNSRFRRKYAAGRFWNKLASHAKTIGRETIEKALYLYYAAQSP
                     DTPAWARRTIYGALGYFIFPFDAVPDLLPVLGYTDDMSVMAAALATVAFCITPDIKEQ
                     ARQKLAQWFDKTPQAGNPVMER"
     gene            2127..>2525
                     /locus_tag="VIR04_01990"
     CDS             2127..>2525
                     /locus_tag="VIR04_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEY9459551.1"
                     /translation="MGRACSFNTYWLAETLRLREAHWGPLEDASEVRRARAQGRGFTE
                     QILLRALFLGQREKLDIVLQKWTQGAKLALAGMTVIALLAGAGAATGALGDGSRSVNL
                     PLALLGLLGLHCLTFVLWLLGFALDRRGGGA"
BASE COUNT          550 a          740 c          713 g          522 t
ORIGIN      
        1 gggagccgct gccagagcga gcgcaagaaa aggttttcta aatgacatga agcgcacccc
       61 aaaagatttc cgcaaacgca accggcccac agaccaccgc cttaaaattc taccattgag
      121 catacaagac aatttattga cactcagcgc catggctgac taagatcgaa agctatcagg
      181 agaactctaa cgtgaaacgc cagtctctgg acatgatcac tgcacttgtc ggctttgaca
      241 cggtgtcgcg caactccaat ttaccgctta ttcattacgt aaaggattac ttatcgcaac
      301 agggggtgca aagccacgtg attcccagcc ccgacggcaa taaggcaaat ctgttcgcca
      361 ccatcgggcc caatgctccc ggcggcattg tcctgtcggg ccacaccgac gtggtgcccg
      421 tggatggcca gctctgggac accgatccct ttcaggtgac tcaaaaaggg gaccggctat
      481 acgggcgcgg cacctgcgac atgaaagcgt ttacggccat cgcactggcg atggcgcccg
      541 acatgctgaa ggcaaaattg aaacggcctg tgcatctggc cctgagctat gacgaagagg
      601 tgggttgcgt gggcgcacct gcccttatcg agcatctgtc cggccacatt gccaggcccg
      661 aaggcgtaat tgtcggcgaa ccaaccagca tgcggcccat cgtggcgcac aaaggaatta
      721 ccgtcctgca caccaccatc acgggccatg aagcccactc cagccaagtg caacgcggcg
      781 tcagcgccgt cacgaccggc gcaaaactga ttacattcat cgacgccatg atggctgaaa
      841 acaaagccgc cgcagacccc gaatgccgtt tcacacccgc ctacaccacg cttcatgtag
      901 gcgtcgtcaa gggcgggacg gcggtgaata tcatttcgcg ccaatgttca tttacatggg
      961 atatccgaac ccttcccggc gattcgtggc gccgttacct tgatcgcttc caggcctatg
     1021 ccgatgagct gttgccggcc atgcgcgccg tctcgcccga cgcggcaatt tcgaccgcac
     1081 tgattgcgca ggcccctcct ctgcaagacc agggcggcgg ggcgcaagac cttgtttacc
     1141 gcgcattggg acattcgaac ggcgatgtcg tgccatacgc cgccgaggcg ggccaatacc
     1201 aggagcgcgg attctcggtc gtgctatgcg ggccaggttc catcgaccag gcgcatcagc
     1261 cgaacgaata cattgaaatc tcgcaagtgc aggcctgcga ggcgtttttc cagacattga
     1321 tcaaacagct tgcgcagtaa aaggtccgcc tggcttaacc ttttgatacc aaatttgcgg
     1381 aaggcggcta gacgatcatt agcggccacc ctatgatgca ttcacgaagg cggcatccat
     1441 tgcctttgcg gtcgtatttt ttccactcta gaaaaagcga gatcgccatg ggtattatca
     1501 gcatgatcat tgtcggactt atcatcggat tgatcgcaag ggcaattatg cccggcgacc
     1561 agaagatggg cattatcctg accattattc tcggtattct cggttcagtg gtcgcgggat
     1621 atctcggcgg agccatcggc tggtatgctc caggggaagg tgcaggctgg atagggtcga
     1681 ttgtcggcgc tatcatcgta ttgttcattt acggtctgat cgccagaaaa aggtaattcc
     1741 gatggacaat tcccggttcc ggcgcaaata cgcagccggg cgcttctgga acaaactcgc
     1801 ctcgcatgcc aaaaccatag ggcgcgaaac gattgaaaaa gcgctttacc tgtattacgc
     1861 agcccaaagc cccgacacgc ccgcatgggc caggcggacg atttatggcg ctttgggcta
     1921 ttttattttt ccgttcgacg ccgtccccga tctgttgccc gttctgggct acaccgacga
     1981 catgagcgtg atggccgcag cgctggcgac tgtcgcgttc tgcattaccc ccgacatcaa
     2041 ggagcaggcc cgccaaaaac tggctcaatg gttcgataaa acgccacagg ccggaaaccc
     2101 ggtgatggag cgctgacgtc ttccacatgg gtcgcgcctg ttcgttcaat acctattggc
     2161 tagccgagac cttgcggctg cgtgaagcgc actggggacc actggaagac gccagcgaag
     2221 tccggcgcgc ccgcgctcaa ggccgcggct ttacagaaca aatcttgttg cgcgcgcttt
     2281 ttttggggca acgcgagaag cttgacattg ttctgcagaa atggacccag ggcgccaaac
     2341 tggcgctggc gggtatgact gtcattgcgc tgctggcggg cgccggggcc gcaacgggcg
     2401 cgctgggcga cggctcccga tccgtgaatc tgccgttggc gctattggga ctgctgggac
     2461 tgcactgcct tacgtttgtg ctttggttgc ttggttttgc cctggaccga cgcggcggcg
     2521 gggct
//
LOCUS       DATCXU010000090         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_424361,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000090 DATCXU010000000
VERSION     DATCXU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424361"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VIR04_01995"
     CDS             <1..1146
                     /locus_tag="VIR04_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9459552.1"
                     /translation="GQKNDTEVIVDHVGIPALATRAAAEVSAQQGHDLFMFLSPPPVY
                     EEQVIDHNEIYQECEKKYGKPVELAVKSTYNPKTKKYFAFSDSFVPDPVNYRKDLWED
                     IGMAAPDTWDDVLTAGRKIKQKSTIPVGIGLSAELDTAMATRTVLFAFGGSVQDEHST
                     VILDSGQTLDAVKFVKALYRDTMTPEVMAWDASSNNRAMLAGKISLCLNAISITREGE
                     NKNIPITPKIQLVQALKGPARRIGMEHVMDCYVIWKFAQNIDGAKKFLVDYIDNFRQG
                     FLGSQFYNFPCFPSTVPDLEKLIGDDPKGQPRDKYKVLGTLMHQATNVGYPGYANAAI
                     DEIFNTWVLNTMFAKAATGADTPENAVKEAHATCKRIFAKWHERGMV"
     gene            1203..>2139
                     /locus_tag="VIR04_02000"
     CDS             1203..>2139
                     /locus_tag="VIR04_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459553.1"
                     /translation="MATLETRELTKRFDGSGSAVDNINLAVRESEFLVLLGPSGSGKT
                     TLLRMIAGLETPTSGDVLVDDQFVTELPPRARNMAMVFQSYALYPHLSVFNNIAFPLQ
                     AQHLARDAISKKVDWAAKLFGIGHLLARKPRQLSGGERQRVALARAVVREPLVFLLDE
                     PLSNLDAKLRISARDELQQFQRRLATTTIYVTHDQIEALGLGDRIVILDHGRVHQLGT
                     PQQVYQHPANTFVATFIGSPPMNLIAEDKRVLGFRPEHFLPADAYPADQPTSLFPFQV
                     TRIENLGSDRLVYGVLESPMPATKVISRIPCTIQTP"
BASE COUNT          525 a          613 c          569 g          432 t
ORIGIN      
        1 ggacagaaaa acgacacgga agtcatcgtc gaccacgtcg gcattcctgc cctggcaacg
       61 cgcgcagcgg cggaagtttc agcgcaacag ggtcatgacc tcttcatgtt cctgtcgccc
      121 ccgccggtct atgaggaaca ggtcatcgac cacaatgaga tttaccagga gtgcgaaaaa
      181 aagtacggca aaccggtcga gctggcggtt aaaagcactt acaaccccaa gacaaaaaag
      241 tatttcgctt tttccgacag cttcgtcccc gacccggtca actatcgcaa agatttgtgg
      301 gaagacatcg gcatggctgc gcccgacact tgggacgacg tattaacggc ggggcgcaag
      361 atcaagcaga aaagcactat accggtggga attggcttgt ctgccgaact ggacaccgcg
      421 atggctacgc gcaccgtgct ttttgcgttc ggcggctcag tgcaagacga gcacagcacc
      481 gtcattctgg attccgggca aactctggac gccgtcaagt ttgtcaaggc cctgtacaga
      541 gacaccatga cgcccgaagt gatggcttgg gatgcttcgt ccaataatcg ggccatgctg
      601 gctggaaaaa tttcactgtg ccttaacgcg atatcgatca cacgtgaagg tgaaaataaa
      661 aatatcccga ttacgcccaa aatacagctc gtccaggcgc tgaaaggtcc tgcaaggcgc
      721 atcggcatgg aacatgtcat ggattgctat gtaatttgga aatttgcaca gaatatcgac
      781 ggtgcaaaga aattcctggt cgactatatc gataattttc ggcaaggttt tctgggcagc
      841 cagttttata acttcccctg ttttccaagc actgttcccg atctggaaaa attaatcgga
      901 gacgacccga aggggcagcc gcgagacaaa tacaaagtac tggggacgtt gatgcatcag
      961 gcgaccaatg tcggctaccc gggttacgcc aatgccgcca tcgacgagat tttcaacacg
     1021 tgggtactca acaccatgtt cgccaaagca gccaccggag ccgacacacc ggaaaacgcg
     1081 gtcaaagaag cgcatgcaac ctgcaaacgc attttcgcca agtggcatga acgggggatg
     1141 gtgtgatgaa cgacttgagc tccaggaagc tatttataga aaacattaac acttaacgcg
     1201 ccatggcgac actcgaaaca cgcgaactaa ccaagcgctt cgacggcagc gggagcgccg
     1261 tcgacaacat caacctggcc gtgcgcgaat ccgaatttct ggtgttgctg gggccgtccg
     1321 gatcgggcaa aaccactttg ctacgaatga ttgcggggct ggaaacccct acttcgggcg
     1381 acgtactggt cgacgatcaa ttcgtgacgg aactgccacc gcgggcgcgc aatatggcga
     1441 tggtgttcca aagctatgct ctctaccccc atttgtcggt attcaataac atcgcgtttc
     1501 cgctgcaggc gcagcatttg gcgcgcgatg ccatcagcaa gaaagtggac tgggcagcaa
     1561 agctgtttgg catcggccat ttgctggcgc gcaagccacg ccaactctcc ggcggggaac
     1621 gtcaacgggt ggccctggcg cgcgccgtcg tgcgcgaacc cctggtattc ctgcttgacg
     1681 agccgctctc caatcttgat gccaagctta gaatttcggc acgcgacgag ctgcaacaat
     1741 tccagcgtcg actggccacg accactatct acgtcaccca cgaccagatc gaggcgctgg
     1801 gcttgggcga tcgcattgtc atactcgatc acggccgcgt ccatcagctc ggcacgccgc
     1861 aacaggtgta tcagcatccg gccaatacct tcgtcgccac gttcatcggt tcgcctccca
     1921 tgaatctgat cgccgaagat aaacgtgtgc tgggctttcg tcccgagcac tttctgcccg
     1981 ctgacgccta cccggccgat caaccaacaa gcttgttccc atttcaggta acgcgcatag
     2041 aaaacctggg ttcggatcgc ctggtgtatg gcgtgctgga atcgcccatg ccagcgacca
     2101 aggtcatatc gcgcatcccg tgcacgatcc aaacaccga
//
LOCUS       DATCXU010000091         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1127648,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000091 DATCXU010000000
VERSION     DATCXU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127648"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..856)
                     /locus_tag="VIR04_02005"
     CDS             complement(8..856)
                     /locus_tag="VIR04_02005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY9459554.1"
                     /translation="MSDVIALVFDFDDTLAPDSTSGFLAEIGVDVADFWSRRVDPLLS
                     QYDWDPVPAYLHEMIELSKSGDHGLITQERLADWGRRLPLHDGVATLFSRLRALVKTE
                     HPQVQIEFYIISSGIGDVVRHTPISLEFTDIWASEFIYDRQGGISFPRRVVSFTDKTR
                     YLFHIQKGIVGPSSRNKPFEVNRKVAEDKLRVPFEQMIFVGDGYTDIPCFSLIRRSGG
                     VAFGVWDPKHREKRSRAWGFIQEGRVSNLNQARYDEQAELYQWLEEAVDSLAGRISLK
                     AGMYRG"
     gene            1044..1880
                     /locus_tag="VIR04_02010"
     CDS             1044..1880
                     /locus_tag="VIR04_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY9459555.1"
                     /translation="MLARIAIHLGRDPGLEHRLRAAIQLATEHDAELVGIYPKNLMRE
                     YAYDENRIPEDVYKILDSRASVEQAKIKEYFFNQTKAAGVAAQWRAPQGPAEETLALH
                     ARYSDLIVMSKAENDDALTSTPVLAKSVIMAAGRPVLMIPSSAGNLASIGQRILFCWD
                     HRREAARAFSDAAPLLRSCSELVILRIDPDNNDLQIKDVESHDFASYCVALGYPQPKE
                     VTLLSDDIGVGNTILNSATDHASDLIVMGAYGHSRMHQWIMGGASRTLLSSMTVPVLL
                     SR"
     gene            complement(1891..>2595)
                     /locus_tag="VIR04_02015"
     CDS             complement(1891..>2595)
                     /locus_tag="VIR04_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY9459556.1"
                     /translation="AARVGAGLVTVAAPASAWAVYAGALTSIMVQPISDADGLDSFAR
                     LLADARKNAIVVGPGAGVSDLTRQCAMAALATRRSVVLDADALSVFGPDPRALFAAVA
                     GPCVLTPHEGEFARLFSCSGDKLSRARDAARRSRCVIVLKGADTVIAAPDGRAAINCN
                     APPELATGGTGDVLSGLIAGLMAQGLAPFSAAAAAVWLHGATASLFGPGLIAEDLPDQ
                     LPLVLRRLHRRAKTPV"
BASE COUNT          615 a          791 c          710 g          479 t
ORIGIN      
        1 agccgtttca gccccggtac atgcccgctt tcaaagaaat ccggcccgcc aggctgtcaa
       61 cggcctcttc aagccattgg taaagctcgg cctgctcatc ataacgggcc tggttcagat
      121 tcgacacccg tccttcctgg atgaagcccc atgcccggct gcgtttttcc ctgtgttttg
      181 gatcccacac cccaaacgcc accccgcccg aacggcgaat caaggaaaaa caagggatgt
      241 cagtgtaacc gtcgcccaca aaaatcattt gttcaaaagg cacccgaagc ttgtcttcgg
      301 ccactttgcg attcacctcg aagggcttgt tgcgcgacga cgggccaacg atcccctttt
      361 gaatatgaaa caaatagcgc gttttatctg taaagctgac aacgcgccgg ggaaaactga
      421 tgcctccttg ccgatcataa atgaactcgg aagcccatat gtcggtgaat tcgagggaaa
      481 tcggagtgtg gcgaactacg tcgccgatac cgctggaaat aatgtaaaac tcaatttgaa
      541 cctgggggtg ttccgtcttg accagcgccc ggagccggct aaaaagcgta gcgacaccgt
      601 catgcaaagg cagacgcctg ccccagtcag ccagacgttc ctgcgtaata aggccatgat
      661 cgcccgattt ggacaactca atcatctcgt gcaagtaggc gggaaccgga tcccaatcgt
      721 attgggacag taaagggtcg acgcgtcgcg accaaaaatc agccacgtcg accccgattt
      781 cggccaaaaa tccagacgtg ctatctggag ccagcgtatc gtcaaaatcg aaaaccaggg
      841 caataacgtc agacatgatc agtcagtttg ggctcgttga aaaccgatac ggcatttagc
      901 ggataaaccc gagtgtaacg cctacccatt gccactaact taagttacag tacgtaagtc
      961 ggctgtgtat ccactccaca gactggaaca tggcaatggc cgccaaggcg gcgccctgca
     1021 tttccacact aacggaggtt gtcatgcttg cacgtatcgc cattcatctt ggtcgcgacc
     1081 ctggtctgga gcatcggtta cgcgcagcta ttcaactggc cacagaacac gatgccgaac
     1141 tcgtcggaat ctatccaaaa aacctgatgc gcgagtatgc gtacgacgaa aaccgcatac
     1201 ccgaggacgt atataaaata ctcgatagcc gggcgtccgt tgaacaggcg aagatcaagg
     1261 aatacttttt caatcaaaca aaagccgccg gggtcgcggc gcaatggcgt gcgccgcaag
     1321 ggccggccga ggaaactctg gcactgcatg ctcgctactc cgatctgatc gtaatgagca
     1381 aggccgaaaa cgacgacgcc ctaacctcta cgcccgtcct ggcgaaatcg gtcattatgg
     1441 ccgccgggcg ccccgtgctc atgattccgt cgtcggcggg aaatctggca tcgattggcc
     1501 agcgtatcct tttttgctgg gatcaccgcc gcgaagcagc aagggcgttt tccgacgcgg
     1561 cgcccctttt gcgatcctgc tccgagttag tgatattaag gatagacccg gacaacaacg
     1621 acctgcagat caaagatgtc gagtcgcacg actttgcgtc gtactgcgtg gcgcttggat
     1681 atccgcaacc caaagaagtc acgctgctca gcgatgatat cggtgtggga aacaccatat
     1741 tgaactccgc caccgaccac gccagcgatc tgatcgtcat gggggcgtac ggccacagtc
     1801 gcatgcacca gtggattatg ggcggcgctt cccgaacatt gctgtcatcc atgacagtgc
     1861 cagttctgct ttcccgctaa agcagcgctt tcacacgggc gtttttgcgc gccggtgcag
     1921 ccggcgcaaa accaaaggca actgatcggg caaatcttcc gcgatcaggc cggggccaaa
     1981 caaagaggcg gtggcgccat gcagccacac ggcggccgcc gctgccgaaa atggcgcgag
     2041 accttgcgcc atcagtcccg cgatcaatcc gcttagcaca tcgcccgtgc cgcccgtggc
     2101 aagttcgggc ggcgcgttgc aattgatggc ggcgcgtccg tcgggcgcgg caatcactgt
     2161 atcggcgccc tttaaaacaa tcacacaacg acttcgccgg gcggcatcgc gggcgcggga
     2221 aagcttgtcg cccgagcagg aaaacaggcg cgcgaactcg ccctcgtgcg gcgtcagtac
     2281 acaggggcca gccacggccg caaaaagcgc acgcggatcg gggccgaata ccgacagagc
     2341 atcggcatca agcaccacac tgcgccgcgt ggcaagcgct gccatcgcgc actggcgagt
     2401 cagatccgat acgcctgccc ccggtccgac gacgatggca tttttccgag catcggccag
     2461 caaacgcgca aagctatcca gaccatcagc atcgcttatg ggctgaacca taatgctggt
     2521 cagtgcgccg gcatacacgg cccaggccga cgcgggggcg gccaccgtaa ccaggccggc
     2581 gccgacgcgg gcagc
//
LOCUS       DATCXU010000092         5295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_25192,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000092 DATCXU010000000
VERSION     DATCXU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25192"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VIR04_02020"
     CDS             complement(<1..842)
                     /locus_tag="VIR04_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY9459557.1"
                     /translation="MSEQVTPPGTEETVSKEALLKAEQYIEEEEGAANIMTGWLGAFL
                     TIVAVVMSVFHMYSAYAIVPTQTLRPVHVGFVLFLSFLMFPIAKRFRNRIVWWDWLAA
                     LLSIGIVVYLLMGGDDFTDRNTSPNAWDIFFGVSLILLVLEAMRRTIGWILPVITVCF
                     LLYAFLGAYLPHPWTHKGYDIARVVGFMYMTLEGIYGVAVDVSSSLIILFTIFGAFLQ
                     FSGAGKFYIDFSFSAMGGKPAGAGRTVVLASFLLGGPSGSGVATTVTLGTVAWPMLSK
                     VGYER"
     gene            complement(962..1921)
                     /locus_tag="VIR04_02025"
     CDS             complement(962..1921)
                     /locus_tag="VIR04_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY9459558.1"
                     /translation="MKISVKQFAYALCFSFAVAQPVVAQNISIGTGGTGGVYYPIGGG
                     IASVLSKHVKGMEATAEVTGGSVDNLKLIDGDVAYIGMAMADATQDAFKGENKFKGHK
                     VPLRTLMIMYPNRMHVVSVEGRNINSVADLKGRHIATGSPGSATEVMALRVLEAAGLD
                     KDKDVKRERLSVAESVNAMKDNKIDAFFWVGGLPTAAVTDLANTPGTKIKMIDHADLV
                     PAMNKKYGNLYVADVIPKSTYRGMDKDNQQSTVMNLLVASEKMDDKTAYNIVKTIFEQ
                     RDDLIAVHRDAAEFKLENQKASASPIPFHPGAVKYFEERGLKF"
     gene            complement(2089..3363)
                     /locus_tag="VIR04_02030"
     CDS             complement(2089..3363)
                     /locus_tag="VIR04_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459559.1"
                     /translation="MTAYFEPRETAPPNERERQLFAHLPDALARACARAPAIARQLQG
                     IDPFSVRARADLERIPVVRKSDLLRVQLAARQGHEAPRDDSVSRVFGGFSAVGWGEAL
                     RVFASPGPIYEIESARPDYWRFARALYAAGFRSRELVHNCFSYHFTPAGSMMETAAHA
                     LGCTVFPGGVGQTEQQVRSIADLAPSCYTGTPSFLKIILERADKLGVALPSLKKALFS
                     AEAFPPSLRQWFLDRGVCGYQAYGSADLGMIAYETHAREGLVVNEDLILEIVRPGTGQ
                     TVTDGEVGEVVVTTLNPDYPLVRFGTGDLSAIMPGLSPCGRTNIRIRGWMGRADQTTK
                     VRGMFVHPGQVAEVVKRHPEIKKARLVVSGRIGADLMTLKVELEAVDPDLEGRIAASV
                     RELTKLRAQVEPSAPGSLPDDGKVIDDIRNYE"
     gene            complement(3378..4208)
                     /locus_tag="VIR04_02035"
     CDS             complement(3378..4208)
                     /locus_tag="VIR04_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459560.1"
                     /translation="MNANVNAETPHMLLNVNGIEVIYNHVILVLKGVSLCVPKGRIVA
                     LLGANGAGKTTTLRAISNLLRAERGDVTKGNIEYRGERIERLTPADLVKRGVVQVMEG
                     RHCFAHLTVEENLLTGAYTRDLSRGDSQAALERVYQYFPRLKHRRISQAGYTSGGEQQ
                     MTAIGRALMADPTMILLDEPSMGLAPQNVDEIFKIVRDLNVREGVSFLLAEQNTNIAL
                     RYADYGYILENGRVMMDGQAKDLAENDDVKEFYLGVSSGGRRNFRDTKFYRRRKRWLA
                     "
     gene            complement(4279..>5295)
                     /locus_tag="VIR04_02040"
     CDS             complement(4279..>5295)
                     /locus_tag="VIR04_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459561.1"
                     /translation="FDTQSTGITFALTDKAFRDKISIETMGYGLSQASDGSVFKWNFP
                     LLGTYWTAADVMIQDIAKKLGGVDKLKGKKIALIYHDSPYGKEPIPLLESRAKANGFA
                     LQLYPVTAPGVEQKSTWLQVRQSRPDYLLLWSAGIMTPTAIREAQAVGYSRDKMYGIW
                     WAGSENDVTDLGQVAKGYNAITIENSAGQGKVHADLKKFVYDKGQGTDTDGKYKGTIA
                     YTRGLMISMLQVEAIRTAQEKFGKGKHMTPEQVQWGFENLNITQDRLNQLGFGEIMRP
                     VKTSCKNHMGDDWARIVQWDGAKFKIVSDWYQSDKSFVDPLVKSEAAKYAKEKNITPR
                     TCEG"
BASE COUNT         1214 a         1545 c         1361 g         1175 t
ORIGIN      
        1 cgttcgtaac ccactttgga cagcatgggc cacgccactg ttccaagcgt caccgtagtg
       61 gcgacccccg aacccgaagg ccctccgaga aggaacgaag caagcaccac ggtgcggccc
      121 gcccccgccg gcttgccgcc catggccgaa aacgagaaat caatataaaa cttgccggcg
      181 cccgagaact gcagaaaggc gccaaagatc gtgaacaata tgatcagcga cgaggatacg
      241 tcgacggcca caccgtaaat accttcgagc gtcatgtaca tgaaacccac aacgcgtgca
      301 atgtcgtacc ctttgtgcgt ccatgggtgc ggcagatagg cgccaagaaa tgcatataaa
      361 agaaagcata cggttataac gggaagaatc cagccaattg tgcggcgcat tgcctcgagc
      421 accagcaaaa tcagggaaac gccgaaaaag atatcccagg cgttgggcga agtattgcga
      481 tcagtgaagt catcgccgcc catgagcagg taaaccacga tgccaattga tagcagcgcc
      541 gcaagccagt cccaccagac gatgcggttg cggaaccgct tggcaatcgg aaacattaaa
      601 aacgacagga ataaaacaaa tccaacgtgc acggggcgca aagtctgcgt cggcacaatg
      661 gcgtaagcgc tgtacatgtg aaataccgac atcactacag ccacgatggt aagaaaagcg
      721 cccagccatc ccgtcatgat gttggcggcg ccctcttctt cttcgatgta ctgttcagcc
      781 ttgagcaggg cctctttgct gaccgtctct tccgtgccgg gcggtgtaac ttgttcactc
      841 ataatgttgc tttctttgag gttgggcggc attgacgcgc caagatttgt tatatgaaaa
      901 aatcaaataa aaacccccaa ccgcaagcgg gttgggggaa accgaaagga aacaaagtta
      961 attaaaactt gaggccacgc tcttcgaagt atttaacagc gccgggatgg aacggtatcg
     1021 gggatgccga agccttctgg ttctcgagct tgaattcggc ggcatcgcgg tgcacggcga
     1081 tcagatcgtc gcgctgttcg aaaatcgtct tcacgatgtt ataggccgtt ttgtcatcca
     1141 ttttttcgct ggccacaagc aagttcataa ccgtggactg ctggttatct ttatccatgc
     1201 cgcgatacgt tgacttggga atgacgtcgg caacataaag gttgccgtat tttttattca
     1261 ttgcaggcac gagatccgcg tgatcaatca ttttgatctt ggttcccgga gtattggcaa
     1321 ggtcggttac tgccgcggtt ggcaacccgc cgacccagaa aaaggcgtcg atcttgttgt
     1381 ctttcatggc attgaccgat tcggccacgc ttaaacgttc gcgcttgacg tccttgtcct
     1441 tgtccagccc tgccgcttcg agcacacgca gcgccatgac ctcagttgcg ctccctggcg
     1501 aaccggtggc aatgtgccgg cctttcaaat cggcgaccga gttgatattg cggccctcga
     1561 cagacaccac atgcatgcga ttcgggtaca tgatcatcag cgtgcgcagc ggcactttgt
     1621 gacccttgaa tttgttttcc cctttgaatg catcctgagt tgcgtcggcc atggccatgc
     1681 cgatataggc cacatctccg tcgatcagct tcagattatc gactgaaccg ccggtaactt
     1741 cggcggttgc ctccataccc ttgacgtgtt tggacaaaac tgacgctata ccgccaccaa
     1801 tgggatagta aacaccgccg gtgccgcctg tgcctatgga tatgttttgc gccaccacag
     1861 gttgcgccac tgcaaacgaa aaacaaaggg cgtaagcgaa ttgttttact gaaatcttca
     1921 tattgactcc gtgaacactg atattgacat gtaccgttgc gtatgacggt acctataaag
     1981 aggcgcagac cccgacaata ctgacacttg cctgattttt tactgacgtc tacctgatgg
     2041 atgtttaacc cgcgagcttc ccttatgtca atcggagtta accacgggtc actcgtaatt
     2101 ccggatgtcg tcaataactt ttccgtcgtc aggcaatgaa cccggggcac tcggttcaac
     2161 ctgagcgcgc aatttggtta gttcgcggac cgatgccgct attcgcccct ccaggtcggg
     2221 gtcgacggcc tccaattcca ctttcaacgt cattaagtcg gcgccaattc gcccacttac
     2281 caccagtcgc gcctttttta tttcgggatg acgcttgacg acctcggcca cctgaccggg
     2341 atgcacgaac atgcctcgaa ccttggtcgt ttgatcggcg cgtcccatcc atcctctaat
     2401 gcgaatattg gtgcgcccgc acggcgacag gccgggcatg atcgccgaca gatcgcccgt
     2461 gccgaaacgc accagggggt aatccggatt caacgtggta acaaccacct cgccaacctc
     2521 gccatcggtc actgtttgtc cggtgccagg ccggacaatc tccaggataa ggtcttcgtt
     2581 gaccaccagg ccttcccgcg cgtgagtctc ataggcgatc atgcccagat ccgcgctgcc
     2641 ataggcctga tatccgcaaa caccgcgatc gaggaaccac tggcgcaggg aaggaggaaa
     2701 cgcttcggcc gagaacaacg ccttctttag agaaggcagc gccacgccaa gtttatcggc
     2761 tctttcgaga ataatcttca agaagctggg cgtgcctgta tagcaagacg gagccaaatc
     2821 ggcgattgaa cggacctgtt gctcggtctg tccgacgccg ccagggaaaa cagtgcatcc
     2881 caacgcatgc gccgctgtct ccatcatgga accagccgga gtgaagtgat aggagaaaca
     2941 gttatggact agttcacgcg aacgaaagcc cgccgcgtac agggctcggg caaagcgcca
     3001 ataatcaggg cgcgcgctct cgatctcata gataggcccg ggcgaggcaa acacgcgcaa
     3061 agcctcaccc caacccacgg ctgaaaatcc gccgaaaacc cggctaaccg aatcatcccg
     3121 cggcgcctcg tgaccttgcc gcgcggcaag ctgaacccgc agcaaatcgc tttttcgaac
     3181 cacagggata cgttcaagat cggcgcgcgc acgcacggaa aaagggtcaa tcccctgcaa
     3241 ctggcgcgcg atggccggcg cgcgcgcaca ggcgcgcgcc aatgcatcag gcagatgcgc
     3301 aaataattgc cgctcgcgct catttggcgg cgcagtctcg cggggttcaa aataagccgt
     3361 catgactttc ccttgtgtta tgccagccac cgtttgcggc gccggtaaaa cttggtgtcg
     3421 cggaagttcc ttcgcccgcc gcttgacaca cccaaataga attctttaac gtcatcgttt
     3481 tcagctaggt ctttcgcctg gccgtccatc atcacccggc cgttttccag gatataaccg
     3541 taatccgcat aacgcagcgc gatattggta ttttgttcgg ccagcaaaaa actgacgcct
     3601 tcacgcacat tcaaatcgcg cacgattttg aaaatttcgt ccacgttctg cggcgccagg
     3661 cccatggacg gctcatccag caaaatcatg gtgggatcgg ccatcagagc gcggcctatc
     3721 gccgtcatct gctgctcacc ccccgacgta tagccagcct ggctgatgcg ccggtgtttc
     3781 aagcgtggaa aatactggta cacccgctcg agcgccgctt gtgaatcgcc ccggctcagg
     3841 tcgcgtgtat aggctccggt cagcaggttt tcttcgaccg tcaaatgagc aaagcaatga
     3901 cgtccttcca ttacctgcac cacaccgcgc ttgaccaggt cagccggcgt taaccgttct
     3961 atacgctcgc cgcggtattc gatattgcct ttggtcacat cgccgcgctc ggcccgcagc
     4021 aagtttgaaa tcgcgcgcag cgtggtcgtc ttgccggccc cgttggcgcc cagaagtgcc
     4081 acaatcctgc ccttcggaac acataaggac acgcccttca gcaccagaat cacgtggttg
     4141 taaataactt cgattccatt gacattaaga agcatatggg gcgtttcggc attcacattt
     4201 gcgttcataa aggcaatcct tgcagtcact gtgagcgggc caccgcatcg cggcgacccg
     4261 tgcgattaca cgactacatt aaccttcgca ggtgcgcggg gtaatgtttt tctctttggc
     4321 gtatttggcc gcctcacttt tcaccaaggg gtcaacaaac gatttatcag actggtacca
     4381 atccgacacg atcttgaact tggcgccatc ccactgaaca atgcgagccc agtcatcgcc
     4441 catgtgattt ttgcaagaag tcttgaccgg gcgcatgatc tcgccaaacc caagctgatt
     4501 cagacgatcc tgggtgatgt tcaggttctc aaagccccat tgcacctgtt ccggcgtcat
     4561 gtgcttgcct ttgccaaatt tttcctgggc agtacgaatt gcttcgacct gcagcataga
     4621 aatcatcagg ccgcgcgtgt aggcaatggt gcccttgtat ttgccgtcgg tgtcggttcc
     4681 ctggcctttg tcgtacacga attttttcag gtccgcatgg actttgcctt gtcctgcgct
     4741 attttcaatc gtgatggcgt tatagccctt ggcaacctgc cccaggtcgg tcacgtcatt
     4801 ctcagaaccg gcccaccaga tgccatacat cttgtcgcgc gaatagccaa cagcctgagc
     4861 ctcacggatc gcggtgggag tcatgatacc ggcactccat agcaacaaat aatccggacg
     4921 gctttgacgc acctgtagcc aggtggattt ttgttcgact ccgggcgccg tcaccggata
     4981 caactgcaag gcgaaaccgt tggctttggc gcgcgattcg agcaacggaa tgggctcttt
     5041 accgtaaggc gagtcatggt aaatcagggc gatcttcttg cccttcaatt tatcgacgcc
     5101 gccaagcttc ttggcgatgt cctgaatcat gacgtcggcc gccgtccagt aggtgcctaa
     5161 caaaggaaaa ttccatttga ataccgagcc atcggacgcc tgcgacaagc catagcccat
     5221 tgtctcgatc gagatcttgt cgcgaaacgc cttgtcggtc agggcaaacg tgatccccgt
     5281 tgattgggta tcgaa
//
LOCUS       DATCXU010000093         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_182192,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000093 DATCXU010000000
VERSION     DATCXU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182192"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VIR04_02045"
     CDS             complement(<1..964)
                     /locus_tag="VIR04_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY9459562.1"
                     /translation="MAVISSFEADSICATALRAAIVRHYSQVRAHTLELAAPLSAEDQ
                     CVQSMPDASPTKWHLAHTTWFFEAMVLSPHLHGYTPFDKRYFYLFNSYYETLGPRHPR
                     PARGLLTRPALPEIRDYRAHVDTAMRRFIESCDEDTWVNIGPLVILGLHHEQQHQELL
                     LTDILHALSCNPLWPAYQPLQSSDLLNRPGAAPALDWLDMPGGVASVGCDAGNGSEFS
                     FDNESPRHDVLLTPYQLANRLATCGEYADFIADGGYHEPRLWLSEGWAAVQAGGWRAP
                     AYWVSSQADDAAPQDWQVFGLHGLLPMNAADPVCQLSFYEAAAYA"
     gene            complement(1142..>2341)
                     /locus_tag="VIR04_02050"
     CDS             complement(1142..>2341)
                     /locus_tag="VIR04_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY9459563.1"
                     /translation="FSEHPKWTPAYGASYIARVLDALTSNPLVWSKTVLFVLYDENDG
                     FFDHVVPPQPPVGPGQGKSTVAFPDELHTVVNPLHDPAYERDGLPYGLGPRVPMTVIS
                     PWSKGGFVCSQVFDHTSVIRFIERRFGVVEPTISAWRRAVCGDLSAALDFTRCDQTIP
                     ILPDTGGYRALVDKQCSVLPKPEAPAAEIPAFQAQEPGIRPARPLPYDLHVRDSLDLR
                     NKRLRLRFDNRGSQGVHFWAYADDTRYAPRHYTVEAQKSLSDDFPTTPSGNYAWTIYG
                     PNGFVRIIKGAFSSAASSDMARLGVQPLYDRRKDVLLVEVRNQSAKGLSVRLTDVAYG
                     NPEQEVALAPASTHLFSLDVKNSGYWYDAALFVTEYSDYIRRFAGHIETGRASCTDPA
                     ARAPVLI"
BASE COUNT          577 a          675 c          640 g          449 t
ORIGIN      
        1 gcgcataggc cgcggcttcg tagaaactca gttggcaaac cggatcagcc gcgttcatcg
       61 gtaacaaccc atgcaggccg aatacctgcc agtcctgagg cgcagcgtca tccgcctgtg
      121 atgacaccca gtaagccggc gcccgccagc cgcccgcctg aactgccgcc cacccttcgg
      181 acagccataa gcgaggctcg tgatagccgc catcggcaat aaagtcggca tactcgccgc
      241 aagtggccaa acgattggcc agttgataag gcgtcaacag gacgtcgtga cgcggcgact
      301 cgttatcaaa tgaaaattcc gacccgtttc cggcatcgca tccgacagag gctacgcccc
      361 ccggcatatc cagccaatcc agtgctgggg ctgcgcccgg ccgattcaac aagtcgctgc
      421 tttggagagg ctgataagcc ggccaaagcg gattgcatga caaggcgtgc agaatatccg
      481 tcagcaatag ctcctggtgc tgttgctcgt gatgcaggcc cagaatcacc aatgggccga
      541 tatttaccca tgtgtcttca tcgcaacttt cgatgaagcg acgcatggcg gtgtcgacat
      601 gtgcgcggta atcacggatt tccggcaacg ccggacgcgt caacagtccg cgcgcgggcc
      661 ggggatggcg tgggcccagc gtctcgtagt aggaattgaa aaggtaaaaa tagcgcttgt
      721 cgaacggcgt gtaaccatgc aggtgcgggc tcagcaccat cgcctcgaaa aaccaggtcg
      781 tatgcgccaa atgccatttg gtcgggctgg cgtcaggcat ggattgaaca cactgatcct
      841 cggccgatag cggagccgcc agctccagtg tatgcgcccg aacctgtgaa tagtgccgca
      901 ctatggctgc acgtagcgct gtagcacaaa tagaatcagc ttcgaacgac gaaataactg
      961 ccataaaaaa ttaacctttg gtctgtgaaa aaaaccgggc gcaatctttt aagttaaggt
     1021 ttattaaaac acggcttcgc acgatagttc tttcgatacg ttaccgtctc aaagcacccc
     1081 caaacatgga tatcccggac taatgcgggc agcggccaat tttgaagaag acgccacccc
     1141 gtcaaatcaa aactggcgca cgtgccgcag ggtcggtgca actggcgcgg cccgtttcaa
     1201 tgtgaccggc aaaacggcga atatagtcag aatactccgt cacgaacagc gctgcgtcat
     1261 accagtaccc actatttttc acgtctaatg agaacaaatg ggtggatgcc ggcgccaatg
     1321 caacctcctg ctcaggatta ccataggcga cgtcggtcag ccgcacagaa agcccctttg
     1381 cgctttggtt gcgcacttcg accagcaaga catcttttcg cctgtcgtaa aggggctgca
     1441 caccaagccg cgccatatct gaagatgcag ccgatgaaaa agcccctttt attatgcgaa
     1501 caaaaccgtt cggcccataa atcgtccacg cgtaatttcc ggaaggcgtt gtgggaaaat
     1561 catcggataa tgatttttgc gcttcgacgg tatagtgcct gggcgcgtac cgcgtatcat
     1621 cggcatacgc ccaaaaatgc acgccctggc tgccgcggtt atcaaatcgc agacgcagtc
     1681 gtttgttgcg aagatccaga ctgtcccgca catggaggtc ataaggcaaa ggtctggcag
     1741 gccgaatgcc gggctcttgc gcctgaaaag ccggaatctc ggcggcgggc gcctctggtt
     1801 tgggcaagac gctgcattgc ttgtccacca acgcgcgata acccccggtg tcaggcaaaa
     1861 tgggaatggt ctgatcgcaa cgggtaaaat ccagcgccgc cgacagatca ccgcaaaccg
     1921 cgcggcgcca ggcgctgata gtcggttcaa cgaccccgaa acgccgttcg ataaaacgga
     1981 taaccgacgt gtgatcgaat acctgcgagc aaacaaaacc gcctttggac catggcgaaa
     2041 tcaccgtcat cggaacccgc ggacccagcc cataaggcag gccgtcgcgt tcgtaggctg
     2101 gatcgtgcaa aggattgacg acggtatgca actcgtccgg aaatgcaacc gtgctttttc
     2161 cctgaccggg gccgacaggc ggctgcggcg gcacgacatg atcgaaaaaa ccgtcatttt
     2221 cgtcatacaa aacaaataga acagtcttgc tccacacgag tggattcgac gtcaatgcgt
     2281 ccagaacgcg ggcgatgtag ctggcgccat aggcgggcgt ccatttcgga tgttccgaaa
     2341 a
//
LOCUS       DATCXU010000094         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1081533,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000094 DATCXU010000000
VERSION     DATCXU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081533"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VIR04_02055"
     CDS             <1..397
                     /locus_tag="VIR04_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY9459564.1"
                     /translation="GQLITQSVLAQFLREGHLTSHIRRMRNLYAERRLGLIEAISTRY
                     GNAVDIVGEHAGLHLVLALPAYSDDHRITHEAFERGVVVRPLSNYYLKASSVKKGLLL
                     GYAAVPIAEIPAAFNTLAEVIDAHLGGGA"
     gene            complement(409..1422)
                     /locus_tag="VIR04_02060"
     CDS             complement(409..1422)
                     /locus_tag="VIR04_02060"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9459565.1"
                     /translation="MNNLPVLGLMLGDMTGIGPEISARLLSANAMKSIAHIVVIGDAR
                     VLELGRRDAGVELKWKTYNNPSAIDWSRPETPLIDLGNIDPATIPRGAVSPESGKQTG
                     ATLKTMIDLALGGEIDAVCFAPLNKAALHKGGWNYHDEHQMFADLTQHVGFFGEMNVI
                     KEFATFRVTSHVALREALAMITTERVMAAVRLANTTLRAMGRTEPRLAVAALNPHAGE
                     GGLFGDEEIRIIQPALERLRAEGVQCSGPVPSDVVFIKALKGDYDGVITMYHDQGQIA
                     TKLLGFNRGVTVTAGLKTVFATPAHGTAFDIVGQGKATTGALEQALRIAANMATARKL
                     TAA"
     gene            complement(1465..2532)
                     /locus_tag="VIR04_02065"
     CDS             complement(1465..2532)
                     /locus_tag="VIR04_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459566.1"
                     /translation="MSFLVLSGLTKRYGPLTVVNNIALSIDKGTLVCLLGPSGCGKTT
                     TLRLIAGFLEPDAGEIHVNGRRISAPGATEAPERRNMSMIFQSYALWPHMTIFDNIAY
                     GLKLRGLKSSEIRQRVDTILEATKLAALAQRYPGELSGGQQQRVSLARALVIEPETLL
                     LDEPLSNLDANLREEMRFEIRRLHDKYHYTTVYVTHDQSEAMTTADQIVVMNQGIIEQ
                     AGTPEDIYQRPQSEFVARFIGGANIFKGRWDKDKTVDCGHGLVLRCDSGDFAEQGDTA
                     VSIRHHSIQLSATQPDGTQPNWISATVVRQSYLGPHRDYLVMLPNGETARTITPIDVV
                     IPKDRTVWLHLPPEHCRALSH"
     gene            complement(2601..>3739)
                     /locus_tag="VIR04_02070"
     CDS             complement(2601..>3739)
                     /locus_tag="VIR04_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9459567.1"
                     /translation="LGARTMITLRRITIPLVLPALLAGALIAALQALTMLGAQAILAL
                     PAGFYVATTKIWSLFQYPPQLELAAAASMPLLLITILLLQGKSWVLGRKGYTVMGGKS
                     GEPRLTHLGKWKPLAVAFAVIILSLTVILPYFALFKTAITHLVSDTLTFKNLTLEHFN
                     FVFFEYSVTRLAMWNTFFLGILTATIGTVIALATAYMVSRNAARGAPLLGILATAPIA
                     IPAIVLGVGFFLTYSHPALPLYGTLWILLLAFLTIAMPAAYQQMSAAFQGVHIELEEA
                     ARILGGSRMSTLFRITAPLLRTTVIATWCFIFIGTIRELSAAILLTTANTKLISVLIY
                     DLNESGDLGAISVLGILLLGVSFIIITLVNRLPGHSAAHLRQVG"
BASE COUNT          754 a         1019 c         1149 g          817 t
ORIGIN      
        1 gggtcagttg atcactcagt cggtgctggc tcagtttctc cgcgaaggtc atttgacttc
       61 acatataaga aggatgcgta atctttatgc cgagcgccgc cttggtttga tcgaagcgat
      121 ctccacgcgc tacggcaatg cggtggatat cgtgggcgag cacgccggct tgcatctggt
      181 cctggcgctt cctgcttata gcgacgatca tcgaattacg cacgaggcct ttgagcgcgg
      241 ggtagtggtg cggcccttaa gtaattatta cctgaaggcc agcagcgtaa aaaagggact
      301 gttgctggga tatgccgcag tgccgattgc tgaaatccct gccgctttca ataccctggc
      361 cgaggtaatc gacgcgcacc ttggcggcgg cgcctgattc catctgcctt aagccgcggt
      421 cagcttgcgc gctgtcgcca tattggctgc gatacgcaaa gcctgttcaa gtgcgccggt
      481 ggtggccttg ccttggccga cgatatcgaa cgcggtgccg tgggccggcg tcgcgaacac
      541 ggttttgagt ccggcggtta ccgtgacgcc tctgttgaag cccagcagtt tggttgcgat
      601 ctggccttgg tcgtggtaca tcgttatcac gccgtcgtag tcgcctttca gtgccttgat
      661 gaacacgacg tccgagggaa ccggcccgct gcactgcacg ccttcggctc gcagccgttc
      721 aagtgccggc tggatgatgc gaatttcctc gtcgccgaac agtccgcctt cacccgcatg
      781 cgggttcaag gctgcaacgg ccaaccgggg ttcggtccgg cccatggcgc gcagcgtggt
      841 attggccagc cgaacggccg ccatgactcg ttcggtcgta atcatggcca gagcctcgcg
      901 tagcgcgaca tgcgatgtca cgcggaaggt ggcgaattcc ttgatcacat tcatttcacc
      961 aaagaacccc acgtgttgcg tgaggtccgc gaacatttga tgctcgtcgt ggtaattcca
     1021 cccgcccttg tgcagcgcgg ccttgttaag cggtgcgaag caaacggcgt cgatttcgcc
     1081 gcccagcgcc aggtcaatca tggttttgag cgtggcgccg gtctgcttgc cggattccgg
     1141 cgataccgcg ccgcgtggga ttgtcgccgg gtcgatgttg cccaggtcga tcagtggcgt
     1201 ttcgggacgc gaccagtcga tggcggacgg gttgttgtat gttttccatt tgagctcgac
     1261 gccggcgtcg cggcgcccca attccagcac ccgtgcgtcg ccaatgacga cgatgtgcgc
     1321 aatagatttc attgcattgg ctgacagcag cctggcgctg atctcggggc cgatccccgt
     1381 catgtcgccc agcatcaggc caagcaccgg caagttgttc atggagatag ttccttggtc
     1441 ttgtagtggt tcttcgtgtg ctttttagtg cgacagagcc cggcaatgtt ctggagggag
     1501 atgaagccag acagtccggt ccttgggaat aacgacatcg atgggcgtaa tggtgcgcgc
     1561 cgtttcgccg ttgggaagca tgaccagata atcacggtgc ggacccagat aagattggcg
     1621 aacgacagtg gcgctgatcc agtttggttg cgtgccgtcg ggttgcgtcg cagacagctg
     1681 aatactgtgg tgccggatgg acacggcggt gtcgccttgc tcggcaaagt cgccggagtc
     1741 gcagcgtaaa accagaccat ggccgcagtc cacggtttta tctttgtccc agcggccctt
     1801 gaagatgttt gcgccaccga tgaagcgagc cacgaattcc gattgcggtc gttggtatat
     1861 gtcttccggc gtgccggctt gctcgatgat gccttgattc atcaccacga tctgatcggc
     1921 ggtggtcatg gcttcagatt gatcgtgggt gacataaacg gtggtgtagt gatacttgtc
     1981 gtgcaggcgc cggatttcga agcgcatttc ttcgcgcagg ttggcgtcca ggttggacag
     2041 cggttcgtcc agcaagagcg tttcaggctc gatgaccagg gcgcgcgcca gcgagacgcg
     2101 ctgctgttgg ccgccggata attcgcctgg ataccgttgc gcaagcgcgg ctaatttggt
     2161 cgcctccaga atcgtgtcga cgcgttgccg gatttcgctg ctttttaagc cgcgcagttt
     2221 aagcccatag gcgatgttgt cgaaaatggt catatgcggc catagcgcat agctttggaa
     2281 aatcatcgac atattgcgcc gctcgggcgc ttcggtggcc cctggcgccg agatgcgccg
     2341 tccattgacg tgaatttcac ccgcatcggg ctcaaggaat cctgcgataa gccgcagcgt
     2401 cgtagttttg ccgcagccgg acggccccag aaggcaaacg agtgttcctt tgtcgatgga
     2461 aagcgcgatg ttgttgacaa cggttagcgg cccataacgc tttgttagac ccgacaacac
     2521 taaaaaactc atggagtttc ccttttcagt taatcgctga agctttcgcc cggcgcttca
     2581 gcggcggagt ttggcgcctt tcagcccact tggcgcagat gtgctgcgct atgtccaggc
     2641 aggcgattga ctagcgtgat gatgatgaac gacaccccta aaagcagaat tccaagcact
     2701 gaaatcgcgc ccagatcgcc gctttcattc aggtcgtaaa tcagcactga aataagcttg
     2761 gtgttggccg tggtcagcaa gatggcggcg gacaattcgc gaatggtgcc gatgaaaatg
     2821 aaacaccagg ttgcaatgac ggtggtgcgc agcaaagggg cggtgatgcg aaacagtgtg
     2881 ctcatgcggc tgccacccaa aatgcgggcg gcttcctcaa gttcgatatg aacgccttga
     2941 aacgcggcgg acatttgctg atacgctgcc ggcatggcga tggtcaaaaa ggccagcagc
     3001 aggatccaca aagtgccgta gagcggcagc gcggggtgcg agtaggtcag gaagaacccc
     3061 acgcccagca caatggccgg aatggcgatc ggggccgtgg ccaatatgcc cagcaacggt
     3121 gcgccgcgcg ccgcgttgcg cgaaaccatg tacgccgtag ccagcgcaat caccgtgccg
     3181 atcgtggccg tgagaatccc caggaagaag gtgttccaca tggcaagccg cgtgacggag
     3241 tattcgaaaa atacaaagtt aaaatgctca agcgtcagat ttttaaatgt cagcgtgtcg
     3301 gacacgaggt gtgttatggc tgtcttgaag agcgcgaaat agggcaggat gactgtcagg
     3361 gacagaatga tgaccgcaaa ggcaacggcc agaggcttcc attttcccaa atgcgtcagt
     3421 cgcggttcgc cgcttttgcc gcccatcacg gtgtagccct tgcggcccaa tacccagctt
     3481 tttccctgga gcaaaaggat ggtgataagc agcagaggca tggaagccgc agcggccagc
     3541 tcgagttgag gcgggtattg aaataaactc catattttgg tcgtggcaac ataaaatccc
     3601 gccggcaaag ccagaatggc ttgcgcgcct aacatggtga gcgcttgcag cgccgcgatc
     3661 aatgcgccgg ccagcagggc cggcagcacc agtggaatcg taattcggcg cagcgtgatc
     3721 attgttctcg cgccgagta
//
LOCUS       DATCXU010000095         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1046702,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000095 DATCXU010000000
VERSION     DATCXU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046702"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="VIR04_02075"
     CDS             <1..1741
                     /locus_tag="VIR04_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEY9459568.1"
                     /translation="CNPAAMAQEHLSGKVGAALDPCAAIRVAFDLDDGQQKEIVFKLG
                     SGSGDDEIRALLQRFKGADAARDELTRVKESWTRALGAIQVETPDKAFDTLANGWLVY
                     QVLASRLWGRTAFYQSSGAFGFRDQLQDVMALVHTEPELVRAHIVLCASRQYPEGDVQ
                     HWWHPPMGRGVRTRCSDDYLWLALASCRYVSATGDVAILDEPIHFLKGRPLDPNEESY
                     YELPAQSPDVATLYEHCVRAITHGLRFGAHGLPLMGTGDWNDGMNKVGAQGKGESIWL
                     AFFLHEVLRQFRSLAWARGDVKFGALCEKEAVALGRNLQVNGWDGAWFRRAYFDDGSV
                     LGSAVNSECQIDSIAQSWSVLSGATSKERMRLAMKAVDERLVQSGSDVIKLLAPPFDV
                     SLPYPGYIQAYVPGVRENGGQYTHAAVWSVMAFAVLRDTQRTWELLSMLNPVNHGGSA
                     EAAALYKVEPYVVASDVYALAPHAGRGGWTWYSGSAGWLYRLMMESVLGIKRRAGKLS
                     IEPCVPIDWASYKISYRFGESVYVITVQQQAGTEVVPRLLLDGVAQDTPFIALVDDGL
                     EHRVQMQLVSLDI"
     gene            1808..>2226
                     /locus_tag="VIR04_02080"
     CDS             1808..>2226
                     /locus_tag="VIR04_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY9459569.1"
                     /translation="MQMGMIGLGRMGSNMVQRLLLRGADCVVYDTHPDAAHELAKKGA
                     VAATSLDELARQLVTPRVVWLMVPASVVDRVLATLVPLLEPGDIVIDGGNSMYQDDIR
                     RAAELGKKKIHYLDVGTSGGTSGFDRGYCLMIGGEAA"
BASE COUNT          437 a          583 c          706 g          500 t
ORIGIN      
        1 ctgtaacccc gccgcaatgg cgcaagaaca tctttcggga aaagtgggag ccgcgctcga
       61 tccgtgtgcg gccatccgtg tcgcgttcga tctggatgac gggcaacaaa aggaaattgt
      121 ttttaaactg gggtcaggct ccggcgatga cgaaatacgc gccttgttgc agcgcttcaa
      181 aggcgcggat gccgcacgcg acgaactgac ccgggtcaag gaatcctgga cgcgcgcact
      241 tggcgcgata caggtcgaaa cgcctgacaa ggcttttgat acattggcca acgggtggct
      301 cgtctatcaa gttctggcca gccgcctgtg ggggcgcacc gccttctacc agtcatccgg
      361 cgccttcggt tttcgcgacc agttgcaaga cgtcatggcg ctcgtccata ctgaaccgga
      421 actcgtgcga gcacacatag tgctgtgtgc ttcgcgccaa tatcccgaag gcgatgtgca
      481 gcactggtgg catccaccca tggggcgcgg tgtgcgcact cgttgttcgg acgattacct
      541 gtggctggct ttggcaagct gccgttatgt ttcggcgacc ggcgatgtgg ccatactgga
      601 cgagccgata catttcctga agggccgccc gcttgacccc aatgaagaat cgtattacga
      661 acttccagct cagtcgcccg acgttgccac gctgtatgag cactgcgttc gggccatcac
      721 ccatgggttg cgctttggcg ctcatggctt gccgttgatg ggcaccggcg actggaatga
      781 cggcatgaac aaggtcggcg cgcagggcaa gggcgaaagc atctggctgg cgtttttcct
      841 gcacgaagtg ctgcggcagt ttcgcagcct tgcgtgggcg cgcggcgatg tgaaattcgg
      901 tgcgctgtgc gaaaaggagg ctgtggcgct tggtcgcaat cttcaagtaa acggctggga
      961 cggggcgtgg tttcgtcgcg cttatttcga tgatgggtct gttcttggtt cggctgtaaa
     1021 cagcgagtgc cagatcgact cgatcgcgca aagctggtct gtgctttcgg gcgcgacatc
     1081 aaaagagcgt atgcgtctgg ccatgaaggc ggtcgatgaa cgtctggttc agtcggggag
     1141 cgacgtgatt aaattattgg cgcccccgtt cgatgtctcc ttgccatacc ccggctatat
     1201 tcaggcctac gttcctggag tcagggaaaa tggcgggcaa tacacccacg ccgccgtgtg
     1261 gtcagtgatg gcttttgcgg tcttgcgtga tacgcagcgc acctgggaat tgttatcgat
     1321 gctcaatccg gtcaatcatg gcggcagcgc agaagcggcg gcgctatata aggtagagcc
     1381 ctatgtcgta gcgtcggatg tatatgcgtt ggcgcctcat gctggtcgcg gaggctggac
     1441 ctggtattcg gggtctgctg gctggctgta tcgcctgatg atggaatccg ttttggggat
     1501 caagcgccgg gccggcaagc taagtattga gccgtgtgtt ccgattgatt gggcatcata
     1561 caaaataagc tatcgttttg gagaatctgt atatgtgatt acggtacagc aacaagcggg
     1621 gaccgaagtc gtgccgcggc ttttgcttga cggcgttgcg caagataccc cgtttatcgc
     1681 gcttgtggat gacgggctgg agcatcgcgt gcaaatgcag ctcgtttccc ttgatattta
     1741 accagcactg atgtttcagc ctggcatgcg ccgggccaat agaggacgtc tgaacaggag
     1801 aacaacaatg caaatgggca tgatcggatt gggacgcatg gggtcgaata tggtgcaacg
     1861 cctgctattg cgcggtgccg attgcgtggt ctacgatacc caccctgacg ccgcccacga
     1921 gcttgcgaaa aagggcgccg tggcggcgac atctttggac gaacttgcac gccagcttgt
     1981 gacgccacgc gttgtatggc tgatggtgcc cgcaagcgta gttgacaggg tgctcgccac
     2041 gctggttccc ttgctcgaac cgggcgatat cgtgattgac ggcgggaatt ccatgtatca
     2101 ggacgacatc cgccgggccg ccgaactggg aaaaaagaag attcattatc tcgacgtggg
     2161 caccagcggc ggcacatccg gatttgatcg cggctattgt ctcatgattg gcggcgaggc
     2221 agccat
//
LOCUS       DATCXU010000096         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1046237,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000096 DATCXU010000000
VERSION     DATCXU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046237"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1210
                     /locus_tag="VIR04_02085"
     CDS             14..1210
                     /locus_tag="VIR04_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459570.1"
                     /translation="MQAPKAPGVAYRVLGAISVAHLLNDMMQSVLLALYPVLQGNFSL
                     SFAQVGVITLAFQFSSSLLQPLIGAFTDKRPLPYSLPTGMGFTMSGLILLSQAWDFPS
                     VVAAAMLIGTGSSVFHPESSRVARMASSGHHGLAQSIFQVGGNLGSSIGPLLAALFIV
                     PFGQKGVVWISLAALLGMVILYRVSRWYSLNLTSVRGRSSLRKTDTGLTRSQVVAAFT
                     LLMVLVFSKFLYLSSLNSYFTFYLMHHFGLTIQNAQYCLFVFLLGVAVGTISGGPVGD
                     RIGRKRIILGSILGAAPFTLILPFANLYWTVALVFVIGVVIASAFPAIIVYAQELAPG
                     RTGAVSGMFFGFAFGIAGIGAAALGMLADKTSIEFVYQVCAFLPLLGIVAFLLPDMRN
                     RKLRLA"
     gene            complement(1263..2141)
                     /locus_tag="VIR04_02090"
     CDS             complement(1263..2141)
                     /locus_tag="VIR04_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY9459571.1"
                     /translation="MRPKDFFLLARDSAVAWGDDYASSMGAAIAFYTVFSIAPLLIIV
                     IAVAGFVWGENAIHGEIFRQLSGLVGADGAAGIQSLLSSASRPKQGLVATTVGVAILV
                     WGATLVFAELQSALDRIWKVPALAKKAGIWNMLRARLLSFGLVLGLAFLLLVSLVVSA
                     ALALLGHWVGGALPDWQVLLQGLNSMVSLAITTVLFAMIFKFMPQADIAWRDVWIGAI
                     VTAILFEIGKILIGLYVGNSSTISALTAAGSFVVVLIWVYYAAQVFLLGAEFTWAYAN
                     RYGSKMSKTATRTTSA"
     gene            2303..>2780
                     /locus_tag="VIR04_02095"
     CDS             2303..>2780
                     /locus_tag="VIR04_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459572.1"
                     /translation="MVTLLFSDIICITQNDTNHVQTMRTLWNQASLLALSTLLALTFN
                     LGSTANAATSSSVKQNSSDVTIKTHDQKKTSKKTVTKKASAKKTVSKKAVVKKTTEEK
                     TVIKKQLVKKTSDKKNTAARRSTRTSAHPAAQAKVQPKLTRVVLRDTGHGHIAPPHR"
BASE COUNT          651 a          823 c          689 g          617 t
ORIGIN      
        1 cgcgacacct gccattcagg ctcccaaagc tcccggagtg gcctatcgtg tgttgggcgc
       61 catcagcgtg gcgcacttgc tcaacgacat gatgcaatcg gttctgcttg cgctctatcc
      121 tgttctacaa gggaacttca gcttaagctt tgcgcaggtt ggcgtaatta cgctggcatt
      181 ccaattctcg tcgtcgctgc ttcaaccgct gatcggcgca tttacagaca aacgtccgct
      241 gccctattca cttccgaccg gcatgggttt caccatgtca ggcctgatat tgctttcgca
      301 agcctgggac ttcccgagcg tggttgcggc cgcaatgctg atcggcacgg gctcatccgt
      361 atttcacccc gaatcgtccc gcgtcgcacg catggcgtca tccggccatc acggcctggc
      421 gcagtccatt tttcaagtgg gcggcaatct ggggtcgtca ataggccctc tgctggctgc
      481 gctgtttatc gttccctttg gtcaaaaagg cgtggtatgg atatcgctgg cggccctgct
      541 gggcatggtg atcctgtatc gggtcagccg atggtactcg ttgaacctca ccagcgttcg
      601 cggacgttca agcctgcgca aaacggatac cggcctgacg cgttcacaag tagttgcggc
      661 gttcaccctt ctaatggtgc tggttttctc gaaattcctt tatctttcca gtctgaacag
      721 ctatttcacg ttttacctga tgcatcactt tggactgacc attcaaaacg cccagtattg
      781 cctgttcgtt ttcctgctgg gagtcgctgt ggggacaata tccggcggac ccgtagggga
      841 ccggatcggc cgcaagcgca taattttggg ttcgattctg ggcgcggcgc cttttacact
      901 gatcttgcct ttcgccaatc tgtactggac cgtcgcgctg gttttcgtca ttggcgtcgt
      961 catcgcctct gcgttcccgg ccattattgt ctacgcgcaa gaactggcgc caggcagaac
     1021 cggggccgtt tcgggcatgt ttttcggctt tgcgtttggg atagcaggga ttggcgcggc
     1081 cgccctgggc atgctggccg acaaaaccag catcgaattc gtctaccaag tttgcgcctt
     1141 cctgccctta ttggggatcg tcgccttcct gctgcccgac atgaggaacc gcaaattaag
     1201 gctggcgtga acgccagcca gcgccaaaag ccggcacacg ccggcggcgc atcggcggcg
     1261 cctcaggcgc tggtcgtgcg cgtcgcggtt ttcgacattt tggaaccgta acgattggcg
     1321 tacgcccacg taaattccgc gcccagtaaa aacacctgcg cggcgtaata tacccagatc
     1381 agcaccacga cgaacgaacc cgccgctgtc aaagcagata ttgtggaact gtttccaaca
     1441 tagagtccaa tcaatatttt gccgatttca aacagaattg ccgtcacaat cgcccctatc
     1501 cagacgtcgc gccacgcgat atccgcctgc ggcatgaatt tgaaaatcat ggcaaaaaga
     1561 accgtggtga ttgcaagcga taccatgctg ttcaggccct gcaaaagtac ctgccagtcg
     1621 ggaagcgcgc ccccaaccca gtgacccagc aatgccagcg ccgcgcttac gaccaaagat
     1681 acgagcaaca gaaaagccag tccaagcacg agcccgaaag aaagcagacg cgctcttagc
     1741 atgttccaga ttccggcctt ctttgccaat gccggcactt tccatattcg atcaagtgcg
     1801 ctttgcagtt ccgcaaagac caaagtggcg ccccaaacaa gaatcgctac tccgactgtc
     1861 gtggccacca acccctgttt gggcctgctg gcgctactga gaagactttg aattcccgca
     1921 gcgccatcgg caccgaccaa accgctcagt tgcctgaaga tctcgccgtg aatggcgttt
     1981 tctccccaga caaaaccggc cacggcaatt acgatgatca acagcggagc gatagagaaa
     2041 acggtataaa atgcaatggc cgctcccatg ctggacgcgt agtcgtcccc ccaagccacc
     2101 gcggaatccc gggccagaag gaaaaaatcc tttgggcgca ttgcgtgctc caattcaagc
     2161 cgtgtggggt gtgctcagca aatttcgtac cacgcgttgc ggcgccacgc gctattatgt
     2221 cagccggaag attttcgcct ggcgcgcctt ctttcccaaa gttaaatttc aatttaacaa
     2281 accgatgtaa ctcactaata tattggtaac tttgttattt tctgatatta tttgcatcac
     2341 ccaaaacgat actaaccacg tacagactat gagaaccctc tggaatcaag catctttatt
     2401 ggctctgtca acgctgcttg cccttacttt taatcttggc tcgacagcga acgccgccac
     2461 atcgtccagc gtaaaacaaa atagttctga cgtcacaatc aaaacccacg atcagaaaaa
     2521 gacctcgaaa aaaacagtca ccaagaaagc ctccgcaaaa aaaacggttt caaaaaaggc
     2581 ggtcgtcaag aaaacaactg aagagaaaac agtcataaaa aaacagcttg taaaaaagac
     2641 aagcgacaag aaaaacaccg ctgcacggcg ctcgacgcgc acttcggcgc acccggccgc
     2701 acaggccaaa gttcagccga aactgacccg ggttgtactg cgtgataccg gccacggcca
     2761 catcgcgccg ccgcatagaa
//
LOCUS       DATCXU010000097         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_783016,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000097 DATCXU010000000
VERSION     DATCXU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783016"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /gene="ruvX"
                     /locus_tag="VIR04_02100"
     CDS             <1..190
                     /gene="ruvX"
                     /locus_tag="VIR04_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY9459573.1"
                     /translation="QAASFRCRRFANQLRGRFGVVVVLVDERGSSIEAQELLGSNADD
                     DAQAAAVILQRYLDALDL"
     gene            complement(193..1029)
                     /locus_tag="VIR04_02105"
     CDS             complement(193..1029)
                     /locus_tag="VIR04_02105"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY9459574.1"
                     /translation="MNSQGDIWVIGDLQGCCTSLNELLMQPELIRNPGDRFWFAGDIV
                     NRGPNSLGSLRRIMEMGDRATVVLGNHDLHLLAVAAGVKGQSASDTLDDILNAPDARE
                     LVDWIRRLPLAHYAQGHLLVHAGVPAPWDVPKTLALAAEVESALRGPDWKDHLEHMYG
                     NKPALWSDDLKGHKRLRVIVNALTRMRLCNGAGEMDFSQKGPPPENGDLVPWFDMPHR
                     LTRDDTVVFGHWSTLGLLVRNDVICLDTGCVWGGKLTALRLRDKKTIQVACVQSQDPT
                     AY"
     gene            complement(1026..2204)
                     /gene="lptG"
                     /locus_tag="VIR04_02110"
     CDS             complement(1026..2204)
                     /gene="lptG"
                     /locus_tag="VIR04_02110"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY9459575.1"
                     /translation="MHTARHYLAREIYRSCAVVLLALIGLFTLFALIDDLDNVGSHFT
                     LINLFYLQALALPTRLYDLLPIGLLIGAILALAGLAQRNELVILRVSGVSGLKLLGML
                     WVITIPMVVGAFLLSEIVTPAAEIKSSEANLILLGRTDGSRLNSGYWFREVTPDNGTR
                     LINIAQLKANGGVAGITLYEFHQGQKLALFAQAKTGRFSKGALILDQVTENQIDARSI
                     SALADAKTPAAPIIRVKLVNQVTLQTTLTPERLIARILTPERMSALDLLDYIGYLRSN
                     HLQTDRQVVALWRKMAYPFTLLVMITIAAPISFSQTRRGGVGSKVFIGILLGVGFFML
                     NQLALNVGMLSKWDPWVTALVPNLGAFGLALAALMTLENQHKVRQFTRARWPWKRPAV
                     "
     gene            complement(2233..2958)
                     /gene="mtgA"
                     /locus_tag="VIR04_02115"
     CDS             complement(2233..2958)
                     /gene="mtgA"
                     /locus_tag="VIR04_02115"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY9459576.1"
                     /translation="MNSRRPRAPKRHLFGGALLLCLGALLLYQLGLFTMIVWYNFQNP
                     GKTAFMNQTLTELRASNPKARLEHEWTPYSGISTSLKRAVIASEDAKFTDHEGVEWDA
                     IRKAWEYNQRQAAEGKHRMRGGSTITQQLAKNLFLSSSRSYIRKGQELVLAYMIELVM
                     SKKRILELYLNVAQWGSNIFGAESAAQHYYRTSAARLNPAQAATLAAMLPNPAYYDEH
                     RSTSYLRSRAGTIEKRMRLAAIP"
BASE COUNT          632 a          844 c          896 g          683 t
ORIGIN      
        1 gcaggctgcc agctttcgtt gccgccggtt tgcaaatcag ttgcgcgggc ggtttggcgt
       61 ggtggttgtg ctcgttgatg agcgaggttc cagtatcgaa gcgcaggaat tgcttggttc
      121 caacgccgac gacgacgcgc aagcggccgc cgtcattttg caacgttatc tcgacgcgct
      181 tgatttataa cgctaatacg ccgtcggatc ctgcgattga acgcatgcca cctgaatggt
      241 ctttttgtct cgcaaccgca gggctgtgag cttgccgccc cagacacacc ctgtgtccag
      301 acaaatgacg tcgttgcgaa ccagcaggcc cagtgtcgac cagtgaccga acacaacggt
      361 gtcatcgcgg gtcaatcgat ggggcatatc gaaccacggc accaggtcgc cgttttccgg
      421 aggtggtccc ttttgagaga aatccatttc tccggcccca ttgcatagcc gcatgcgcgt
      481 cagcgcattg acgataacgc gtaaacgttt gtggccctta agatcatcgc tccagagggc
      541 cggtttattg ccgtacatat gttccagatg gtctttccaa tcgggtccgc gcaaggctga
      601 ctcaacctcg gcagcaagcg ccagtgtttt cggaacatcc cagggcgccg gcacgccagc
      661 atgaacgagc agatggccct gcgcgtagtg agctagcggc agacgccgaa tccagtccac
      721 aagttctctg gcgtcaggcg cgttcagtat gtcgtccagc gtatccgaag cgctttgtcc
      781 ttttacaccg gcggccaccg caagcagatg caggtcatgg ttgcccagca cgacggtcgc
      841 ccggtcgccc atttccatta tgcgtctaag ggaaccaagc gaattgggtc cgcgattgac
      901 gatatcgccc gcgaaccaga atcggtcgcc gggattccgg atgagttcag gctgcattaa
      961 tagttcattc agggacgtgc agcagccctg cagatcgccg attacccaaa tgtcgccttg
     1021 cgaattcata cggccggcct tttccatggc cagcgggctc tggtgaactg gcgaaccttg
     1081 tgctgatttt ccagcgtcat caacgccgcc agagcaagtc cgaaggcccc cagattgggt
     1141 acgagcgctg tcacccaagg atcccacttg cttagcattc cgacattcag ggcaagctga
     1201 ttgagcatga agaaccccac gcccagcagt attccaataa atactttact gccgaccccg
     1261 ccccgtctgg tttgcgagaa gctgataggc gcggcaatgg tgatcattac cagcaaagtg
     1321 aacgggtagg ccattttccg ccatagtgcg acgacttgac gatcggtttg caggtggttg
     1381 cttctaaggt aaccaatata gtccagcaga tccagcgccg acatgcgctc gggggtcaaa
     1441 atgcgggcga tcaggcgttc aggggtaaga gtggtttgca gcgtcacctg gttaaccagt
     1501 ttgacccgga taataggagc ggctggggtc ttggcgtcag ccagcgctga aatggagcgc
     1561 gcgtcgatct ggttttcggt gacctggtcc agaataagcg cgcccttgct aaagcggccg
     1621 gttttggcct gggcgaataa agccagtttt tggccttggt ggaattcgta aagagtaatg
     1681 ccggccacgc ccccgtttgc cttcagttgc gcgatattta tgaggcgcgt gccattgtct
     1741 ggcgtcactt ctctgaacca gtatccgcta ttgagacggc tgccgtcggt gcgccccaat
     1801 agtatcagat tggcctcgct ggattttatt tcggcggcgg gggtgacgat ctcggagagc
     1861 aggaacgcgc cgacaaccat cgggatagta atgacccaca gcatacccag caatttcagg
     1921 ccgctgaccc cggagacgcg caatataacc agctcgttgc gttgcgccag ccccgccagc
     1981 gccagtatgg cgccgattaa cagcccgatg ggcaatagat cgtacagacg cgtaggcaga
     2041 gcaagggcct gcaagtaaaa cagattgatc agggtgaagt gggagcccac attatccaga
     2101 tcgtcgataa gcgcgaatag agtgaagagt ccgatcagcg ccagcaaaac gacggcgcag
     2161 gagcgataga tttctcgggc aaggtagtgg cgggcggtat gcatcggaaa tggttttaat
     2221 gtcttgtcgg atttagggta ttgcggccag acgcatccgc ttttcgatcg tgcctgcgcg
     2281 agatcgcaga taggacgtgc tgcgatgttc gtcataatac gccgggttcg gcagcatggc
     2341 ggccaacgtg gccgcctggg cggggttgag cctggccgcg ctggtgcgat agtagtgttg
     2401 agctgcgctt tcggccccga aaatgtttga tccccactgg gctacgttca aatacagttc
     2461 cagaatgcgc tttttgctca tgaccagttc aatcatatac gccaaaacca attcctgccc
     2521 cttgcgaata tacgagcgcg agctggacaa aaaaagattt ttggcgagct gctgggtaat
     2581 cgtcgagccg ccgcgcatgc ggtgcttgcc ttccgcggct tgccgctggt tatattccca
     2641 ggctttgcgg atggcgtccc attcgacgcc ttcgtgatcc gtaaatttcg cgtcttccga
     2701 cgcgatgaca gcgcgtttga gcgacgtact gattccagaa tagggcgtcc actcatgctc
     2761 gagcctggcc tttgggttgg atgctctcag ctcagtcagg gtctgattca tgaatgctgt
     2821 tttgcccggg ttctgaaagt tgtaccagac tatcatggtg aacaagccta gctgatacag
     2881 cagtagcgcg ccaaggcata gcagtagtgc gccgccgaac agatgtcgct tgggcgcacg
     2941 aggccttcgg ctgttcatgc cgtccgggca gcgcgaattc gttgccgtag tgcggccagc
     3001 accggttctg tgtcgggcat taccccgtgc cagattgcga agctttcggc tgcct
//
LOCUS       DATCXU010000098         7687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_464096,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000098 DATCXU010000000
VERSION     DATCXU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464096"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VIR04_02120"
     CDS             complement(<1..632)
                     /locus_tag="VIR04_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9459577.1"
                     /translation="MKDKCVLVTGATKGIGWAISQRLADLGCHVVGLARHTQDVDFPG
                     YLYSCDLNNAGETEDILRVIREKYPVDAVVNNVGLVLPELLGEVELASLYQVFDVNVR
                     VAVQVTQAFVASMKARKEGRIVNICSRVAEGSVGRTSYSAAKSALIGCTKTWALELAE
                     FGITVNAVSPGPIETELFRVNHPVGSDVEKKALASIPMGRLGQPADVAAAV"
     gene            complement(675..1808)
                     /gene="alr"
                     /locus_tag="VIR04_02125"
     CDS             complement(675..1808)
                     /gene="alr"
                     /locus_tag="VIR04_02125"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY9459578.1"
                     /translation="MPRPISAVISLQSLSHNLAVVAKRLNGAGPGSRQAVPRIWAVIK
                     ANAYGHGIANALQGFEQADGLAMLDLNEAVRCRDAGWDKPILLLEGFFEPSDIGVVDR
                     YRLSLVVHCQDQLDMLDSASVDHPIDAFIKLNSGMNRLGFSEANFQSAYRRALAAQQR
                     GILGALGKMTHLARADDDAAITTQQIQLFNETTGRLPGKVSVCNSAAVLTPGLWAQVS
                     GEGEQWVRPGICLYGASPFADKTASSLGLLPAMTLSARLIGVQTLVKGAGVGYGHTFI
                     APCAMRVGVVACGYADGYPRHASTGTPITVEGVLTRVLGRVSMDMLAVDLTPAPGAHV
                     GSTVIMWGRGGPSVDDVARAAGTIGYELLCAVAPRVPKTVVMM"
     gene            1949..2776
                     /gene="hpnC"
                     /locus_tag="VIR04_02130"
     CDS             1949..2776
                     /gene="hpnC"
                     /locus_tag="VIR04_02130"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY9459579.1"
                     /translation="MTVNHYENFPVASLLLPRRLREPVRHIYNFARSADDIADEGNAT
                     APERLQQLNAYQAALSQIQAGELALSDSDPRHAIFDPLARTITQFSLPLAPFFNLLSA
                     FMQDVTIHRYLDDAALFDYCSRSANPVGRLMLHLYNEDTSEQNLIQADCICTGLQLTN
                     FWQDIAVDWEKSRVYLPQQKLEYYGIDEQNMARHQSSEAWRALMHAQVQQAREQLMEG
                     FPLARRLPGRIGLELRLVVHGGLRILERIELLHYDVFRNRPVLQKQDWILLLLRALR"
     gene            2819..3682
                     /gene="hpnD"
                     /locus_tag="VIR04_02135"
     CDS             2819..3682
                     /gene="hpnD"
                     /locus_tag="VIR04_02135"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY9459580.1"
                     /translation="MSPDEYCQEKAAQSGSSFYYSFLFLPPERRKAITALYAFCREVD
                     DVVDECTDASVARMKLAWWRSQIDQMFDGNAEHPVTRALAPHTQSCRITAERLYAVIE
                     GMEMDLDQTRYEDWSGLRKYCWCAAGVVGELSAGIFGYTQTGTLAYAEKLGLAFQLTN
                     IIRDVGDDAHRGRIYLPADEMKQFDVKAAEILDGQYSDRFVQLMRFQTERAKQFYREA
                     VQTLPDVDRRAQRPGLMMAAIYYTLLEEIERDNWHVLDQRISLTPIRKFWLAWKTWVG
                     GGQGIIKRMSR"
     gene            3679..4995
                     /gene="hpnE"
                     /locus_tag="VIR04_02140"
     CDS             3679..4995
                     /gene="hpnE"
                     /locus_tag="VIR04_02140"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY9459581.1"
                     /translation="MKVAVVGAGWAGLSAAVYLHRYGQDVVVFEAARTLGGRARSTHS
                     PKLKATIDNGQHILLGGYSETLKLFRDLGINPDSHLHRQPLSLRTADGSFSLNAPSLP
                     APFHLLAALVKGHGLSLKERAKLLASCCILRLYEWKTPPGQTVAQWLTKNHQSEHIVR
                     QFWQPLCLAALNTPIDRACAQLFAYVLRDSLGGPSSDSNVLIPSVDLSEIWAEHLPAQ
                     IDIRYGRVVKKLIYNDSHTQIDNEEFDNVVLATNAYAAHRLLKTLPPSETGKRYLELL
                     QAFEYLPIATITLQLERPWKLPYSMLMLRDNPARLQFGQWLFDRSTFMQDPTKRKNSS
                     LTVVISDARALSEHAHDEIVAAVITQIEEQTVRFPAMPKVTGHELICEKRATFAAIPN
                     LQRPATQTPWPGIWAAGDWTNTRYPAVLEGAVRSGKHAATLVTRDF"
     gene            complement(4992..5375)
                     /gene="crcB"
                     /locus_tag="VIR04_02145"
     CDS             complement(4992..5375)
                     /gene="crcB"
                     /locus_tag="VIR04_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY9459582.1"
                     /translation="MLTFGHLFAIGIGAAAGAVSRWLLGLWLNHSGVMMPWGTLAANL
                     IGGYLIGVVLGVIAIYPATPAWARLLLMTGFLGGLTTFSTFSGETIGMIERGSYAIAL
                     GYVGISLLGTLALTVLGMATVHAMH"
     gene            complement(5383..6792)
                     /gene="thrC"
                     /locus_tag="VIR04_02150"
     CDS             complement(5383..6792)
                     /gene="thrC"
                     /locus_tag="VIR04_02150"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY9459583.1"
                     /translation="MKYRSTRGGMALQSFSDILLEGLAPDGGLTLPEALPRLDMEVIE
                     SWRGLSYPELATEVLGLFIDDIPKDDLRRLVHTAYGPGVFASADVVPLKPLSEGVSLL
                     GLSEGPTLAFKDLAMQILGQLFEYVLAQRGATLNILGATSGDTGSAAEYALRGKRGIA
                     VFMLSPHGRMSDFQRAQMYSLQDPNIHNLALDGVFDECQDIVKSLASDLAFKSAYHLG
                     AVNSINWARIAAQVVYYFWGWLRATSAPGLAVSFAVPSGNFGNILSGHLAREMGLPIR
                     RLVLATNENNVLEEFFRTGVYRPRAASQTVATSSPSMDISRASNFERFVFDLVGQDPA
                     RVRRLWQQLSDDGEFDLSELLPLLKNRYGFYSGVSTHADRLATIKSVYDTTGVLIDPH
                     TADGVKVAGRYTEPGVPMVVLETALPAKFSKTIEQAIGRTPQPPANLRNLKDLPQRVQ
                     VMDCDAAQVRQYIVRHALG"
     gene            complement(6789..>7687)
                     /locus_tag="VIR04_02155"
     CDS             complement(6789..>7687)
                     /locus_tag="VIR04_02155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY9459584.1"
                     /translation="PIVKAIREGLTANRIQWVAGIINGTTNFILSEMRTRGLTFDQAL
                     AQAQSLGYAEADPTFDIEGIDAAHKLALLASLAFGVPVQFSKAYVEGITQLAREDIVH
                     AERLGYRIKLLGITKWRTDGIELRVHPTLVPIDRLLANVEGAMNAVLVKGDAVGATLY
                     YGQGAGELPTASSVVADLVDVTRLLAADPEHRVPYLAFQPDAMSDTPILPISEVISAY
                     YLRLRVKDAPGVLADLARILSDCEISIGTMFQQPHGDQDAEIIFLSHEACEGAVDRAI
                     GKIQSLPFVTSPVTRLRVENLL"
BASE COUNT         1815 a         2315 c         2018 g         1539 t
ORIGIN      
        1 acggccgcgg ccacatcggc cggctgcccc aggcgtccca tgggaatgga tgcgagcgct
       61 tttttttcca catcgcttcc aacggggtga ttgacgcgga acaattctgt ttcgatcggg
      121 ccaggcgata ccgcgttcac cgtaattcca aattcggcga gttcaagagc ccaggtttta
      181 gtacagccaa taagagcgct tttggccgcg gagtaactgg tccggccgac ggatccttcg
      241 gcgacccggc tgcaaatatt aacgatgcgg ccttctttgc gcgccttcat cgaagccaca
      301 aacgcctggg tgacttgcac cgccacgcgc acatttacat cgaagacctg gtacaaagag
      361 gcaagttcca cctcgcccaa aagttcgggc agcaccagac ccacattatt aaccactgcg
      421 tcgacaggat atttttcgcg aatgacgcgc aagatatctt ccgtttcgcc ggcattgttc
      481 aaatcgcaag aataaaggta gcccggaaaa tcgacatctt gggtatgacg cgccaaccct
      541 acaacatggc accccaaatc agccaaacgc tggctgattg cccagccaat tcccttggtg
      601 gctcctgtca ccagcacaca tttatccttc acggtttttc cttaaaaaaa acttggttcg
      661 cactgtcatt gacgctacat cataacaacc gttttcggca cacgcggcgc caccgcgcaa
      721 agcaactcgt agcctatcgt acctgcggcg cgggcaacgt cgtccaccga aggcccgccg
      781 cgcccccaca tgattacagt actgcccaca tgcgctccgg gcgcgggcgt caaatcaact
      841 gccagcatat ccatggagac ccgcccaagc acgcgcgtaa gcactccctc cacagtaata
      901 ggcgtgccag tggatgcatg gcgcggatag ccatccgcat agccgcaggc cacaacccct
      961 acccgcatgg cgcacggcgc aataaacgtg tgtccgtagc ccacgcccgc ccccttgacc
     1021 agcgtctgca ccccgatcag gcgcgcgctc agtgtcatgg ccggaagcaa acccagactg
     1081 ctggccgtct tgtcggcaaa aggcgaggcg ccgtataaac aaatgccggg tctgacccac
     1141 tgctcgcctt cgcccgacac ctgcgcccaa agcccaggcg tcaagacagc cgccgaattg
     1201 cacacactga ccttcccggg cagtcggccg gtcgtctcgt taaacaactg tatctgctgc
     1261 gttgtaatgg ccgcgtcatc gtctgcgcgc gccaaatgag tcattttgcc cagcgcaccc
     1321 aaaatgcccc gttgttgcgc cgccagcgcc cgccgatagg cgctctggaa attggcttca
     1381 gaaaaaccca ggcgattcat gcccgaattc aacttgatga atgcatcgat cggatgatcg
     1441 acgctggctg aatccagcat atcaagttga tcctggcaat gcacaacaag agacaggcgg
     1501 taccggtcga cgaccccaat atcgcttggc tcgaaaaaac cttcgagcag caaaatcggc
     1561 ttgtcccacc cggcatcgcg gcaacgaacc gcctcgttca ggtccagcat cgccaaaccg
     1621 tcggcctgtt cgaagccctg gagcgcgttc gcaatgccat ggccgtacgc gttggccttg
     1681 atcaccgccc aaatccgcgg aacagcctga cgcgagccag ggccggcgcc attcaatcgt
     1741 ttggcgacga ccgccaggtt atgacttaag gactgcaacg aaataacggc cgagattgga
     1801 cgtggcataa ctacctcctg atagccgaag tctaatgcag ttccggcgca aatgtgaaag
     1861 cacgtgcgcc aacagggcac gcacggctat ccgcccagta acagcggcta tcttttaaac
     1921 taacgtttcc gatccagcca ggcccgccat gaccgtcaat cactacgaaa attttccggt
     1981 cgcatcgctg ctcttgccgc gccgcctccg cgagcccgtc aggcacattt acaatttcgc
     2041 ccgcagcgcg gatgatatcg ccgatgaagg caacgcaacg gcgcctgaac gattgcagca
     2101 actgaacgcc tatcaagccg cactgtcgca aatacaagca ggcgagctcg ctctttcgga
     2161 cagtgatccc aggcacgcca ttttcgatcc actggccaga accattacgc agttctcatt
     2221 gccgcttgca ccttttttca atttgctatc ggcgttcatg caagatgtaa cgatacaccg
     2281 ctacctcgat gatgccgcgt tgttcgacta ttgctcacgt tcagccaacc ccgtcggacg
     2341 tttaatgctg catctataca atgaagatac atcggaacag aatctgatac aggcggactg
     2401 tatttgcacg ggactacaat tgactaattt ctggcaggat attgctgttg attgggagaa
     2461 atcccgggtt tatctgcctc aacaaaagct tgaatactat ggcattgacg agcagaacat
     2521 ggcacgccat caatccagcg aggcatggcg cgcactgatg catgctcaag tgcaacaagc
     2581 gcgtgaacaa ttaatggagg ggttcccgct ggcccgccgc ctgccggggc ggattggact
     2641 ggaattgcgg ttggtcgtgc atgggggctt gcgtattctt gaacggatag agctgttaca
     2701 ctatgacgtt tttcgtaacc gtcccgttct gcaaaagcag gactggatcc tgttgttatt
     2761 gcgcgctttg cgctagtcaa cggccgagct gtgcttgcaa attagaaact gataacgcat
     2821 gagccccgac gaatattgtc aagaaaaggc tgctcaaagc ggctccagct tctactactc
     2881 gttcttgttt ttgccgcccg aacgccgcaa ggcaatcact gcgttatacg ccttttgccg
     2941 cgaagtcgac gacgtggtcg acgaatgcac cgatgcgtca gtggcacgca tgaaactggc
     3001 gtggtggcgc tcacaaatcg accaaatgtt cgacggaaac gccgaacacc cggtaacgcg
     3061 ggcattggcg ccccacaccc aatcatgtcg tataacggcc gaacgtctgt atgccgtcat
     3121 cgaaggcatg gaaatggacc tggatcaaac acgctacgag gattggtccg gcttgcggaa
     3181 atattgctgg tgcgccgcgg gggtcgtggg cgagctctct gcaggcatat ttggttacac
     3241 gcagaccgga acattggcgt atgccgaaaa gctggggctg gcttttcagt taaccaacat
     3301 tatccgcgac gtcggtgacg atgcacaccg cggacgcatt tatttgcccg ccgacgaaat
     3361 gaaacagttt gatgtcaaag cggctgaaat tctggacggg caatactccg accgtttcgt
     3421 gcaattgatg cgttttcaaa ctgagagggc gaaacagttc taccgcgaag ccgttcagac
     3481 tctgcctgac gtggatcggc gcgcgcaacg ccctggcctg atgatggccg ccatctacta
     3541 cacccttctg gaagaaatcg aacgcgataa ctggcatgtt ttagaccagc gcatctcact
     3601 gaccccaatc cgcaagtttt ggcttgcctg gaaaacatgg gtgggcggtg ggcaaggcat
     3661 tataaaaagg atgagtcgat gaaggttgcc gtagtcggag caggctgggc cgggctaagc
     3721 gccgcggtat atttgcatcg ctatggccag gatgtggtgg tttttgaggc cgcgcgcacg
     3781 ctgggagggc gagcgcgcag cacacattct ccaaaattga aagccaccat tgacaatggc
     3841 cagcacattt tgttgggcgg ctattccgaa acgctcaagc tctttcgcga ccttggcatt
     3901 aaccccgatt cgcacttgca ccggcaacca ctttcgcttc gaaccgccga cggcagcttt
     3961 tcactaaacg cgccatcgct gcccgccccg tttcatttgc tggcagctct cgtcaaaggt
     4021 catgggcttt ccttaaaaga gcgcgccaaa ctgcttgcca gttgctgcat actgcggctg
     4081 tacgaatgga aaacgccccc agggcaaacc gtcgcgcagt ggctcacgaa aaaccatcag
     4141 tccgagcata ttgtcaggca attttggcaa ccattgtgtc tggcggccct gaacacgcca
     4201 attgaccgcg cctgcgccca actgttcgct tacgtgctgc gcgacagcct gggcggaccc
     4261 agcagcgatt ccaatgtttt gatccccagc gtcgatctgt cggaaatatg ggctgaacat
     4321 ctgccagccc aaatagacat ccggtacggc cgggtcgtca aaaaattgat ttataacgat
     4381 tcgcataccc agatcgacaa cgaagaattc gataacgtcg tgcttgccac aaatgcctac
     4441 gcggcccatc gtctgttgaa gacgcttccg ccttccgaaa ccggcaaacg ctatctggag
     4501 ctacttcaag ccttcgaata tctgcccatc gcgaccatca ctttgcagct tgagcgccca
     4561 tggaagcttc cctattccat gctgatgcta cgggacaacc cggccaggct acagtttggt
     4621 caatggctat ttgatcgcag tacgttcatg caagacccga ccaaacgcaa aaattcatcg
     4681 ttgacggtcg tcattagcga cgcgcgtgcg ctcagtgagc atgcgcacga cgagatcgta
     4741 gccgccgtga tcacacaaat cgaagaacaa acagtgcgct tcccggccat gcccaaagtc
     4801 acgggacacg aactgatatg cgagaagcgg gcgacgtttg cggccattcc caacctgcag
     4861 cgccctgcca ctcagactcc ctggccaggc atctgggcag cgggagactg gaccaacaca
     4921 cgctatccag ccgtgctcga aggggccgtg cgcagcggca aacacgctgc cactttagtc
     4981 acccgggatt tttaatgcat cgcgtgaacg gttgccatgc ccaagaccgt caaggccagc
     5041 gtgcccagta agcttattcc gacataaccc agcgcgattg catacgaacc gcgttcgatc
     5101 atgccgatcg tttcacccga aaatgtcgaa aatgtcgtca agccgcccaa aaatcctgtc
     5161 attagcagca atctcgccca agcgggcgtc gcggggtaaa tggcaataac gcccaggacc
     5221 accccgatca gatagccgcc tatcagatta gccgccaggg tgccccacgg catcatcacg
     5281 cccgaatgat tcagccaaag ccccagcagc cagcgactaa cggcgccagc agcagcgcca
     5341 atgccgatcg caaaaaggtg gccgaatgtc agcacggttt atttatccca aagcatgccg
     5401 cacaatatac tgacggacct gcgcagcatc gcaatccatg acctgtacgc gctgaggcag
     5461 atctttcaga ttgcgcaaat tcgccggcgg ttggggggta cgcccgatag cttgttcgat
     5521 cgttttactg aatttggccg gcagcgcggt ttcaagcacc accatcggaa cgcctggctc
     5581 ggtgtagcgc ccggccacct tgaccccatc cgcggtatgc ggatcgatca atacaccggt
     5641 cgtgtcgtag acggatttga tcgttgccag acgatcggca tgcgtgctga cgccgctgta
     5701 aaaaccatag cggtttttca gcaagggaag cagctcgctc aaatcgaatt ccccgtcgtc
     5761 cgaaagctgc tgccacaatc gcctgacgcg ggccggatcc tggccaacca ggtcaaatac
     5821 gaaacgctca aaattggacg cacgtgaaat gtccatggat ggactggatg ttgcgaccgt
     5881 ctggctcgcg gcgcgcggcc tgtatacacc cgtgcggaaa aactcttcaa gaacattgtt
     5941 ctcgttggtg gccagcacca gacgtcgaat gggcaacccc atttcgcgcg ccagatggcc
     6001 ggatagaatg ttgccaaagt tcccggaggg aaccgcaaaa gaaacggcca gcccgggcgc
     6061 tgatgtcgcg cgcaaccagc cccagaagta atacaccacc tgtgcggcga ttcgcgccca
     6121 attgatggaa ttgacggctc ccagatgata ggccgacttg aatgccagat cgcttgccaa
     6181 agacttgaca atgtcctggc attcgtcgaa taccccatca agcgcaagat tatggatatt
     6241 tggatcttgc agcgagtaca tttgcgcacg ttgaaaatcg ctcatgcgtc catggggcga
     6301 cagcataaaa accgcaatgc cgcgttttcc acgcaaagca tactcggcgg ccgaaccggt
     6361 atcgcccgag gtggcgccca gaatattcag agtagcgccg cgctgggcca ggacatattc
     6421 aaacagttgg cccagtatct gcatggccaa atctttaaat gccaacgtcg ggccttcgga
     6481 caggccaagc agactgacgc cctcacttaa gggtttaagc gggacaacat cggcgctggc
     6541 aaacacgccg ggcccatatg ccgtgtgcac caggcggcgc aaatcgtcct tgggtatgtc
     6601 gtcaataaac aggcccaata cttctgtggc caattccgga taacttaaac cgcgccacga
     6661 ctcgatgact tccatgtcca atcgcggcaa cgcttcaggc agcgtcaggc ccccgtcggg
     6721 cgccaggcct tcgagcaaga tatcggaaaa cgattgaagc gccatgccgc cgcgcgttga
     6781 acgatatttc atagaaggtt ttccactcgc aaacgcgtaa ccggggacgt cacaaacggc
     6841 agggattgaa tcttcccgat ggcgcgatcg acagcccctt cgcacgcttc atgcgacaaa
     6901 aagataatct ccgcgtcttg atctccgtga ggctgctgaa acatcgttcc tatggaaatc
     6961 tcgcaatcgg acaaaatgcg cgcaaggtcg gccagcacgc caggagcgtc tttcacgcgc
     7021 aagcgcaaat agtacgccga gataacctcg gaaatcggca aaattggcgt atcggacatg
     7081 gcgtcgggtt gaaaggccag atacggcacc cgatgttcgg gatcggccgc cagaagccgc
     7141 gtcacatcga ccaggtcagc gaccacggac gaagccgtgg gaagctcgcc ggcgccttgc
     7201 ccataataca aggtcgcacc caccgcgtcg cccttgacca gcacggcgtt catcgcgccc
     7261 tctacattgg ccagcaatcg atcaataggc accagcgtcg ggtgcacccg cagttcgatg
     7321 ccatcggtgc gccatttggt gattcccagc aatttaatcc gatagcccag gcgttccgca
     7381 tgaacgatgt cctcccgggc caattgcgtt atgccttcca cgtaagcttt gctgaactgg
     7441 acaggaaccc cgaaagcaag tgaagcaaga agggccagct tatgggccgc atcgattccc
     7501 tcgatgtcga aggtggggtc ggcttcggca taccccagac tttgagcctg agccagcgcc
     7561 tgatcgaaag tcaggccgcg cgtacgcatt tccgacaaaa tgaaattggt ggttccgttg
     7621 ataatgcccg ccacccactg aatgcggttg gcggtcaaac cctcgcggat cgccttcaca
     7681 atcggaa
//
LOCUS       DATCXU010000099         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_813745,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000099 DATCXU010000000
VERSION     DATCXU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813745"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VIR04_02160"
     CDS             complement(<1..1365)
                     /locus_tag="VIR04_02160"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY9459585.1"
                     /translation="MNAAADPQPLVDLNAPDWVRNVHLKEWVAEIAALTQPDRIEWCD
                     GSQQEYDRLCELMVKAGTLRRLNPAKRPNSYLAWSDPSDVARVESRTFICCEKEDDAG
                     PTNNWTEPAAMRKTLNGLFAGCMRGRTMYVIPFSMGPLGSPIAHIGIELSDSPYVVAN
                     MRLMTRMGRKVYDVLGADGEFVPCVHSVGKPLAAGQADVSWPCNDIKYIVHFPESRQI
                     WSFGSGYGGNALLGKKCLALRIASTMGRDQGWMAEHMLILGVTSPQGKKMHVAAAFPS
                     ACGKTNFAMMIPPAAFPGWKITTVGDDIAWIKPQSDGRLYAINPEAGYFGVAPGTNEQ
                     TNFNCMASLRENVIFTNVALTDDGDVWWEGMGPEPKHCLDWHGEDWTPASGRKAAHPN
                     ARFTVAAVQNPVVDPEWDNPHGVVIDAFIFGGRRSDTVPLATEARSWQEGVYMAATLG
                     SQTTA"
     gene            complement(1443..2087)
                     /locus_tag="VIR04_02165"
     CDS             complement(1443..2087)
                     /locus_tag="VIR04_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY9459586.1"
                     /translation="MIEIVVIGPQPLRRLGIARVLSAISGARIEEADARFATQETCVG
                     RKVDLAVISVSPATDLAEVMRALRDRYSPKSVLLLSDAAAASYCTPYHPNISGCIPKS
                     STSAVLIASVRLILSGGLCFSAPIPAAALQPPALGPANGEASLLRLTEGQYEVLVLLA
                     RGYPVKTVSRQLNIPIAAGKAHARSLYQRLNAHGKTEALRAAVSRGAKLGRIPD"
     gene            2200..>2411
                     /locus_tag="VIR04_02170"
     CDS             2200..>2411
                     /locus_tag="VIR04_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459587.1"
                     /translation="MKKQNPVWPLRWDLLLRYRLIEIVAQWEGRLTTNHLCDSLGIGR
                     QQASKDINSYLKDIAPGNLVYDRHLKG"
BASE COUNT          543 a          720 c          665 g          483 t
ORIGIN      
        1 agccgtcgtt tgagaaccga gcgtagccgc catatagacg ccctcttgcc aactgcgggc
       61 ctcggttgcc aacggcaccg tgtcggagcg gcgaccgcca aaaatgaaag cgtcgatgac
      121 tacaccatgg ggattgtccc attccggatc caccaccgga ttttgcacgg cggcaacggt
      181 gaaacgcgca ttcggatgtg cggccttccg gcccgatgcg ggggtccagt cttcgccatg
      241 ccaatccaga caatgcttgg gctcggggcc cataccttcc caccagacat cgccgtcatc
      301 ggtaagcgcc acattggtaa atattacgtt ctcgcgcagc gacgccatgc agttgaaatt
      361 ggtctgctcg ttggtgccgg gagccacgcc gaagtaaccc gcttccggat tgatggcgta
      421 caggcggcca tcggattggg gcttgatcca cgcaatatcg tcgccaacag tggtgatttt
      481 ccaccccggg aaggctgcgg gcggaatcat catggcgaag ttggtcttgc cgcaggctga
      541 cggaaaagcc gccgccacgt gcatcttttt tccttgcggc gaggtgaccc ccaaaataag
      601 catgtgctcc gccatccagc cctgatcgcg gcccattgtg gaagcaatac gcagtgccag
      661 gcactttttg cccagcagcg cgttgccgcc gtagcccgaa ccaaacgacc agatttgccg
      721 gctttccgga aaatgcacaa tatatttaat gtcgttgcac ggccacgata cgtcggcctg
      781 gccggcggcc agcggtttac ccaccgaatg cacacacggg acgaactcgc catcggcgcc
      841 caggacgtca tataccttgc ggcccatacg cgtcataagg cgcatgttgg ccaccacata
      901 aggcgaatcg gagagctcta tgccaatatg cgcaatgggc gagcccagcg ggcccatcga
      961 aaaaggaatc acatacattg tgcgcccacg catgcagccc gcaaacagac cattcagcgt
     1021 ttttcgcatg gccgccggct cagtccaatt gttggtgggg ccggcgtcgt cctctttctc
     1081 gcaacagata aaggtgcggc tttccacgcg cgccacgtcg ctgggatcag accaggccag
     1141 gtaggaattg ggccgtttgg cagggttcaa gcggcgcagc gtgcccgcct tcaccatcaa
     1201 ttcgcacaat cggtcatatt cctgctgcga accatcgcac cattcgatgc gatcaggctg
     1261 agtcagtgcg gcgatctccg cgacccactc tttaagatgc acattgcgca cccagtcggg
     1321 agcattcaaa tccactaaag gttgggggtc ggcggcggcg ttcataggaa atgggactcc
     1381 ataataatga atggcatttg catgaaagcg ccattttaag gcttatcgaa ggaaaaaacc
     1441 cgctaatccg gaatccggcc cagtttggcg cctcgggaaa ctgcggcgcg cagggcttcg
     1501 gtcttgccat gcgcattcag gcgttgatac aacgaccttg catgcgcctt tcccgctgca
     1561 atcgggatat tcaactggcg acttaccgtt ttaacgggat aaccgcgcgc caggagcacc
     1621 agtacctcgt attggccttc ggtaaggcga agcaagcttg cttcgccatt cgccgggccg
     1681 agcgccggcg gttgcagcgc ggcagccggg atgggcgccg aaaagcacag cccgcccgac
     1741 aagatcaagc gcacggacgc aatcagcaca gcggaggtcg aactcttcgg aatgcagccc
     1801 gaaatattcg ggtgatatgg cgtgcagtac gatgccgcag cggcgtccga caataacaac
     1861 accgatttgg gagagtatct gtcccgcagg gcgcgcatta cttcggccag atcggtagcc
     1921 ggtgaaaccg agatcacggc caaatccact ttcctgccaa cacacgtttc ctgcgtagcg
     1981 aaacgggcgt cggcctcttc gatccgcgcg cccgaaatgg cagaaagaac ccgcgcaata
     2041 cccaatcgcc gcaaaggctg aggtccgatt acaacgattt cgatcatttt tatcccattg
     2101 cctggtcatt tgccggcatt tcgggaatat gatatgtcta catcaacgac atgcaaatac
     2161 tgtatacata tatatgtacg cctttcacac aggtgtttat tgaaaaagca aaatccggtc
     2221 tggcccctgc gctgggatct gctgcttcgt taccggctga tagaaatcgt cgcccaatgg
     2281 gaaggtcgcc tgaccaccaa tcatttgtgt gacagcctgg gcatagggcg ccagcaggct
     2341 tccaaggata tcaacagcta ccttaaagac atcgcccccg gcaacctggt gtacgaccgc
     2401 catttgaaag g
//
LOCUS       DATCXU010000100         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_947180,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000100 DATCXU010000000
VERSION     DATCXU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947180"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VIR04_02175"
     CDS             complement(<1..475)
                     /locus_tag="VIR04_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459588.1"
                     /translation="MDAFGLFAASGIKKTPHLLRPRLSRRRFVNGSAAFAAMLSASAL
                     ASTSASSQPSVFDNKNTGDEPAVIFRGGRIYTVNAAEPWAEAVAIRGKKIVAVGADAQ
                     VSKLAGPATRVVELNGRMMLPGFVEGHIHPFLGSFFTEGIDLQVPTLDEALDVIAR"
     gene            complement(598..1596)
                     /locus_tag="VIR04_02180"
     CDS             complement(598..1596)
                     /locus_tag="VIR04_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459589.1"
                     /translation="MFFTRVATIAIFCAAASASFLVQAKTEPPAWPAGRAIRVIVGYP
                     AGGVSDTVTRALAENITGKLKTPVVVENHGGAAGSIGMDIVAKAAPDGYTLGFSSISP
                     LALNPHLMKPTFDPEKDIVPVVSVMYSPIVLLATGASDVKDVKDFPALIALAREKPGM
                     VTWATSGKGSVGHIMVEQIMRAAKVSILDVPYKGAGQQLQDALGGQFDVMSVNSAPML
                     AQQIKSGKLRPLAVAAPERLPAYPNQPTFAELGYPGANLSSLFGIFAPKGTPEPIIKR
                     VNEAVNEALKDPKIAGILANSDNVATGGTPESFKAEIQRQSEGNARIIKEAHITLQ"
     gene            complement(1631..3109)
                     /locus_tag="VIR04_02185"
     CDS             complement(1631..3109)
                     /locus_tag="VIR04_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459590.1"
                     /translation="MTSLTAIASHAPASPSLAADIPARLDRLPWSAWHWRVVIALGVA
                     WVLDGLEVTIVGSFGSILERPETLGLTAAEVGLAGSLYIAGAVIGALVFGRLADTLGR
                     KRFFLVTLTVYMIATLATGFSTRDWFFVLCRFATGLGIGGEYAAINSAIDELVPARVR
                     GRVSLAINGSFWIGAALGAGLSLLVLDETVIPPEWGWRLGFILGAVLALAIALVRRNV
                     PESPRWLLIHGRGNEAECIVAGIESQVGARHGALPPAPQRARTRPDGRVVPRLGEIAH
                     VLLHTYRRRSAVALALMVSQAFFYNAIFFTYALVLTRFYGVHDNRVGLYIFPFALGNV
                     LGPLLLGPLFDRVGRRKMIAITYILAGSGLALTAWAFLEGWLNARSQALCWSAVFFMA
                     SAAASSAYLTVSEVFPLEMRALAISLFYAVGTGIGGVVGPVLFGVLIESGRPVAVAGG
                     YAFAAVLVVAAGFTALRFGVNAERCSLEDIAPPLAGSRRLNA"
     gene            complement(3228..3509)
                     /locus_tag="VIR04_02190"
     CDS             complement(3228..3509)
                     /locus_tag="VIR04_02190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY9459591.1"
                     /translation="MAQATARHILVSTEAKCKELKTAIENGADFAQVAKDNSSCPSSR
                     DGGNLGSFGPGQMVKEFDAVVFSAPVNVVQGPVKTQFGYHLVEVTSRQD"
     gene            complement(3524..4660)
                     /locus_tag="VIR04_02195"
     CDS             complement(3524..4660)
                     /locus_tag="VIR04_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY9459592.1"
                     /translation="MEWTSRSDIATVIHRILRSRRFIAAAMLLAMTVAGAVNTSERAA
                     KSSPGATHISLLRLVGDYPLPGRATRWDYMSLDPGESRLFLAHLGDSSVVVVDTKTKT
                     VVATINGIGEPHGVLAIPELGHVYVSATKTNEVVAIDATTLRVTARIPGGVYPDGMAY
                     APEVHKLYVSDEAGGTETVIDVRSNKRVATIRLGGEVGNTQYDPVSKRIFVNVQGTRE
                     LIEIDPSTDTVVQRTTLVGADGNHGLLIEPARRLAFIACEGNGKLFVMNLNTHKIVSR
                     FGTGKGPDVLAYDAGLGLVYVASESGTVSQFKLSEQGLTKSGEGFVGPNAHVVAVDPA
                     THEVYFPLKKVGRQPVLRVMKPVPGSIAASARSSAISGILFDFI"
     gene            complement(4693..5088)
                     /locus_tag="VIR04_02200"
     CDS             complement(4693..5088)
                     /locus_tag="VIR04_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459593.1"
                     /translation="MNAKLLLGASLAALAVGFGGAGVASAQGGNPAPNMQNQSTGMGG
                     AMGSDDSSSMGQQKRGMHRGRMQRGMMDGCPMMGGMMDGMMDGMMGWAGGANAKVMMQ
                     MHGEMMRAMGDIMIKYADKIDMTPPVKMP"
     gene            5260..>5453
                     /locus_tag="VIR04_02205"
     CDS             5260..>5453
                     /locus_tag="VIR04_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HEY9459594.1"
                     /translation="MIEKTQKQKMLAGELYVANDPELTADNRRISEWLDRYNATNTMA
                     QPERQALLEEALGAVGSGCN"
BASE COUNT         1252 a         1661 c         1459 g         1081 t
ORIGIN      
        1 gccgcgcgat cacatcgagc gcttcgtcca aggtcggcac ctggagatca attccttcgg
       61 tgaagaagga gcccagaaag ggatgaatat ggccttccac gaagccgggc agcatcatac
      121 ggccattaag ctcgacaact ctggtcgccg gcccagccag tttgcttacc tgcgcatcgg
      181 ctccgaccgc cacaatcttt ttgccgcgga tggcaaccgc ttcggcccat ggctcggccg
      241 cgttaaccgt ataaattctg ccgcctctga agatcaccgc cggctcgtcg cccgtgttct
      301 tattatcgaa cacggatggc tgcgacgacg cggaggttga agctagagcc gaggccgaca
      361 acatggcggc gaaagccgcg ctcccgttga cgaaacgacg gcggcttaat cgggggcgaa
      421 gtaaatgtgg cgtcttcttt atgccgctgg cggcaaatag cccaaaggca tccatcaggt
      481 tgcagctcat gcacatagtc attccctcgt tagcatagtg attgcgcgtt aggacggctc
      541 tttattgttt gacggacgcc ggtgtgagcg cgttgactgc tagtatagcg gcggccttca
      601 ctgcaaggtg atatgggctt ctttgatgat tcgggcgttg ccttcggact ggcgctggat
      661 ttcggccttg aatgattcgg gcgttccgcc ggttgcgacg ttgtcgctat tagccagaat
      721 acctgcaatc ttcggatcct tcagtgcctc gttgaccgcc tcatttacgc gtttgatgat
      781 cggctcgggc gtgcccttcg gtgcgaagat gccgaacaat gaggacaagt ttgcccccgg
      841 gtaacccagt tcagcaaacg tgggctgatt cggatatgcc ggtagccgct cgggtgcggc
      901 gaccgccagc ggacgcaact tgccggactt gatctgctgc gccagcattg gcgccgagtt
      961 caccgacatc acatcaaact gccctccgag cgcgtcctgc agttgctggc ccgcgccctt
     1021 gtatgggaca tccaggatgc tgactttggc tgcacgcatg atctgttcga ccatgatatg
     1081 tccaaccgac cccttgcccg aagtcgccca agtcaccatg ccgggctttt cgcgggccag
     1141 cgcaatcaga gccgggaaat ccttgacgtc cttgacgtcc gaagctcccg tcgccagcaa
     1201 cacaatcggg gaatacatca cactgaccac cggaacaatg tccttttccg gatcaaaagt
     1261 cggcttcatc aaatgcgggt tcaaggccag agggctgatc gaagaaaaac ccaacgtata
     1321 accgtctgga gcggccttgg ctacgatatc cattccaatg cttccggctg ctccgccatg
     1381 attttcaaca acgaccggcg ttttcagctt gccggtgatg ttctcggcca aggcgcgagt
     1441 gaccgtgtcg ctgacacccc ctgccggata acccacaatc acacgaattg cacgaccggc
     1501 gggccaggca ggcggctcgg ttttcgcctg caccagaaag ctggccgatg cggctgcgca
     1561 aaagattgcg atagtggcaa cacgagtaaa aaacataatg ggtttccggt aatggatgta
     1621 aatcggaatt ttacgcgttc agccgccgcg aaccagcaag tggcggagcg atatcttcaa
     1681 gagaacagcg ttccgcattg acgccgaagc gcaatgcagt aaagccagcg gcgacgacca
     1741 gcactgccgc gaaagcatag ccgccggcta ccgcgacagg ccggccgctt tcaattaaaa
     1801 cgccgaaaag cacaggtccg acaacgcctc cgatgcccgt gccaacggca tagaacaagg
     1861 agatggccaa agcgcgcatt tctaatggga aaacttcgct gaccgtcaga tacgccgaac
     1921 tggctgcggc cgacgccata aaaaaaacag cggaccagca tagcgcctga ctgcgggcgt
     1981 tgagccagcc ttcaagaaat gcccacgccg ttagcgcaag ccccgaaccc gccagaatgt
     2041 aggtaatcgc gatcattttg cgccgcccga cccggtcgaa cagaggtccc agcagtaacg
     2101 gccccaaaac attgcccagc gcgaacggaa aaatataaag ccctacgcga ttatcatgca
     2161 caccataaaa gcgtgtgagc accagggcat aggtaaaaaa tatggcgtta taaaaaaacg
     2221 cctgcgacac catcagagca agggccacag cgctgcgacg acggtatgta tgcaacagca
     2281 catgcgcaat ttcgcccaga cgcggcacga cgcggccatc gggacgagtc cgcgcacgct
     2341 gcggcgccgg cggcagcgcg ccatgccggg cgccaacttg ggattcgata ccggcgacga
     2401 tgcactcagc ttcattaccc cggccgtgta ttaacaacca ccgtggactc tcgggcacat
     2461 tgcgacgaac cagcgcaatc gccaaggcca acacggcgcc caggataaac cccaggcgcc
     2521 aaccccactc cggaggaatg actgtttcat ccagcaccag caggcttaag cccgcgccga
     2581 gcgccgcgcc tatccagaag ctgccgttga tcgcgaggct gactcggccc cggacgcgcg
     2641 ccggaaccaa ctcgtcaatc gcggaattga tggctgcgta ctcgccccca ataccaagcc
     2701 cggttgcgaa ccggcacaaa acaaagaacc agtcccgggt cgaaaacccc gtagccagcg
     2761 tcgcgatcat atagacagtc agcgtgacca gaaaaaatct cttgcgaccc aacgtatcgg
     2821 ccaatcgtcc gaaaaccagc gcgccgatta ccgcgccggc aatataaagc gaccccgcca
     2881 aaccgacctc ggcggccgtc aatcccagcg tttccggccg ctcaagaatg ctgccaaacg
     2941 accccacgat ggtcacttca aggccatcca acacccaggc cacgccaagt gcaatcacaa
     3001 cacgccagtg ccaggccgac cacggcaagc ggtcaaggcg agccgggatg tcggcggcca
     3061 ggctgggcga agcgggtgca tgggatgcga tggcggtcaa tgaagtcatc cacagaatcg
     3121 cgacgactta tcttacgccg ataacgccat cgaccgagcc cgtggctcac gaccttgttt
     3181 tagttaagtt cctaaagcag gagcttcttg atacaagaaa agtatcgtta atcctggcgg
     3241 ctggtgactt cgaccaagtg ataaccaaac tgggttttta ccggaccttg cacaacattg
     3301 accggcgcgc tgaacacgac tgcgtcaaat tccttcacca tttggccggg gccgaacgaa
     3361 cccaaattgc ccccgtcacg gctggacggg caactggaat tatcttttgc aacctgcgcg
     3421 aaatcagccc cattttcaat ggcggtttta agttccttgc attttgcttc agtggaaacg
     3481 aggatgtgac gagcggtggc ctgagccatg agcattactc cttttaaatg aagtcaaaga
     3541 gaatacctga aatagcggaa gaacgcgcag acgcggcgat tgaaccggga accggcttca
     3601 ttacccgcaa caccggttgg cggccaactt tctttaatgg gaaatagact tcatgagtcg
     3661 cgggatcaac cgccaccaca tgggcgttcg gccccacaaa cccctctccg gacttggtca
     3721 ggccttgctc gcttagcttg aattgcgata ccgtccccga ttcgctggcg acatacacta
     3781 agccaagacc ggcatcgtag gccaatacat cggggccttt gcctgtaccg aaccgtgaaa
     3841 caatcttgtg tgtgttcaga ttcatcacga acaacttgcc gtttccttcg catgcaataa
     3901 atgcgagcct ccgtgccggc tcaatcaaca ggccgtggtt gccgtcggcc ccgacaagcg
     3961 ttgttctttg caccacggta tcggtgcttg gatcgatttc aattagctcc cgcgttcctt
     4021 gaacgttgac aaatatgcgt ttggaaacgg gatcatattg cgtgttgccg acttcgccgc
     4081 ccagcctgat ggtggccacg cgtttgttgg accgcacgtc aataaccgtt tccgttccgc
     4141 cggcttcgtc cgagacataa agcttgtgca cttccggcgc gtaggccatg ccgtcgggat
     4201 agacgcctcc gggaatacgc gcggtaacgc gtaatgttgt cgcatcaatg gccactactt
     4261 cgttcgtctt tgttgcggac acataaacat ggcccagctc ggggatcgca agcaccccgt
     4321 gcggctcgcc aataccgtta atggtcgcga ccactgtctt ggttttcgtg tccacaacca
     4381 ccacggagct atcgcccaaa tgcgcaagaa acaggcgcga ctcgcctggg tccaggctca
     4441 tatagtccca acgcgttgcc ctcccgggta gcggatagtc gccgaccaag cgcagaagcg
     4501 atatatgcgt tgcgcctgga cttgacttgg ccgcgcgttc actggtgttc actgcgccgg
     4561 cgacggtcat cgcaagcagc atggccgcgg cgataaagcg acgcgatcgt aaaatgcggt
     4621 gaatcactgt cgcgatatcg ctgcgggaag tccattccat gatgtgcccc agcacacccg
     4681 cgctcaagcc tttcagggca tttttacggg cggcgtcatg tcaatcttgt cggcatactt
     4741 gatcatgatg tcgcccatgg cgcgcatcat ctcgccgtgc atttgcatca tcacttttgc
     4801 gtttgccccg cccgcccagc ccatcatgcc gtccatcatg ccgtccatca tgccacccat
     4861 catcggacag ccgtccatca tgccgcgttg catcctcccg cgatgcatgc cacgcttttg
     4921 ttggcccatt gacgatgagt catcgcttcc catggcgccg cccatgcctg ttgattgatt
     4981 ctgcatattt ggcgcgggat tgccgccttg cgcgctagcc acccccgcgc cgccaaaacc
     5041 caccgcaagc gcggctaagg atgcaccaag caataatttc gcattcattc gtatctctcc
     5101 tgaatttaac aattcggcca ttggcgtaga gacgaaacgg cgcaaaaatt tggccggaaa
     5161 tcttgccaat caagtataag ctttggggta gcgtggccac cataagctgt tacctattcg
     5221 aacaaccgtt ttggcccgta acacaatggt ggaaactata tgatcgagaa gacgcagaag
     5281 caaaagatgc ttgccggaga gctttatgtt gcaaacgacc ctgagctcac cgccgacaac
     5341 cggcgcattt ccgaatggct tgatcgctac aacgcgacaa acactatggc acagccggaa
     5401 cgacaggcgc tgctggagga ggcactggga gcggtcggtt ccggatgcaa cat
//
LOCUS       DATCXU010000101         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_340644,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000101 DATCXU010000000
VERSION     DATCXU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340644"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VIR04_02210"
     CDS             complement(<1..454)
                     /locus_tag="VIR04_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY9459595.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEA
                     RGYAQIDAAPEEKARGITINTSHVEYETKTRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPFIIVYMNKADMVDDKELLELVE"
     gene            complement(526..>2617)
                     /gene="fusA"
                     /locus_tag="VIR04_02215"
     CDS             complement(526..>2617)
                     /gene="fusA"
                     /locus_tag="VIR04_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY9459596.1"
                     /translation="TPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKLGEVHDGAA
                     TMDWMEQEQERGITITSAATTAFWSGMAGDRPQHRINIIDTPGHVDFTIEVERSMRVL
                     DGACMVYCAVGGVQPQSETVWRQANKYGVPRLAFVNKMDRTGANFFKVYEQLKTRLKA
                     HPVPIVIPIGAEENFTGVVDLVKMKGIIWDEASQGIKFEYIDIPPELLDQANEWHEKL
                     LESAAESTEELMNKYLETGSLSQDEITLGIRTRTIAGEIQPMLCGTAFKNKGVQRMLD
                     AVVDYLPSPVDIPPVEGQDDEGNVVTREANDSAKFSALAFKLMSDPFVGQLTFIRVYS
                     GVLKSGDTIYNPIKGKKERVGRILQMHSNNRAEIKEVLAGDIAAVVGLKDVTTGETLC
                     DVGSHVLLEVMDFPEPVISQAVEPKTKADQEKMGIALSRLAQEDPSFRVRSDEESGQT
                     IISGMGELHLEVLVDRMRREFGVEANVGKPQVAYRETIRKTCAEVEGKFIKQSGGRGQ
                     YGHVVLKLEPLPPGGGYEFVDAIKGGVVPREFIPAVDKGIRETLPAGVIAGYPVVDVK
                     VTLFFGSYHDVDSNENAFRMAASMAFKDGMRKASPVLLEPMMAVEVETPEEYAGTVMG
                     DLSSRRGMVQGMDDMAGGGKAIKAEVPLAQMFGYSTSLRSQTQGRATYTMEFKHYAEA
                     PKNVADEVVSARGK"
BASE COUNT          520 a          799 c          698 g          600 t
ORIGIN      
        1 tctccaccag ctccagcaac tccttgtcgt ccaccatgtc cgccttgttc atgtagacga
       61 tgatgaaagg cacacccacc tggcgcgcca gcaggatgtg ctcgcgcgtc tgcggcatcg
      121 ggccgtcggc ggccgatacg accagaatcg cgccgtccat ctgggccgca cccgtgatca
      181 tgttcttgat gtagtccgcg tgccccgggc agtccacgtg cgcatagtga cgcgtcttcg
      241 tctcgtattc cacgtgcgag gtgttgatcg tgatcccgcg cgccttctct tcaggcgccg
      301 cgtcaatctg cgcatacccg cgcgcttctc cgccaaactt cgacgccaac accgtcgtga
      361 tcgccgccgt caacgtcgtc ttgccgtggt caacgtgacc gatcgtccct acgtttacgt
      421 gcggtttggt ccgctcaaac ttgccttttg ccatgattta ttccaccaat gttttttcag
      481 gaaacaaaaa gttttcccgc ctgccacaag gcaggcggga tcgtgttact tgccgcgtgc
      541 gctgacaact tcatcagcga cattctttgg agcttcggcg taatgcttga actccatggt
      601 gtaggtcgcg cgaccttgcg tttgtgaacg caagctggtc gaataaccaa acatttgagc
      661 caagggcacc tcggccttga tggccttgcc gccccccgcc atatcatcca tgccctgaac
      721 catgccgcga cgcgaagata aatcgcccat cacggtgccg gcatattcct cgggcgtttc
      781 gacttcgacc gccatcatgg gctcaagcaa taccgggctg gctttgcgca tgccgtcttt
      841 aaacgccatg gaggccgcca tgcggaacgc gttttcgttc gaatccacgt cgtggtatga
      901 accgaagaac agcgttacct tgacatcaac caccgggtag cctgctatga cgccagcggg
      961 caatgtttcg cgtatccctt tatcgaccgc cgggatgaac tcgcggggaa ccacgccgcc
     1021 cttgatggca tcgacgaatt cgtaaccgcc gccaggcggc aaaggttcaa gcttgagcac
     1081 cacgtggcca tactggccgc gaccgccgga ctgtttaata aacttgcctt cgacttcggc
     1141 gcacgtcttg cgtattgttt cccggtaggc cacctgaggt ttgcccacat tggcctcgac
     1201 gccgaattcg cggcgcatgc gatccaccaa cacctcaagg tgaagctcgc ccatgccgga
     1261 aataatggtc tggcccgatt cttcgtcgct acgcacacgg aaagaaggat cttcctgggc
     1321 gagacgcgac aacgcaatac ccattttttc ctggtcggcc ttggtcttgg gctcgaccgc
     1381 ctgcgaaata acgggttcgg gaaaatccat gacttcgagc aaaacgtgcg aaccgacgtc
     1441 gcaaagtgtt tcgccggttg taacgtcttt aagacccacg acagccgcga tatcgcccgc
     1501 caaaacttct ttgatctcgg cgcggttgtt tgagtgcatt tgcaaaatac ggccgacgcg
     1561 ctctttcttg cccttgatcg ggttgtagat cgtatcgccg gatttcaaga cacccgaata
     1621 aacgcgaatg aacgtaagct ggcccacaaa agggtcgctc atcaacttga acgccagcgc
     1681 cgagaacttg gcgctgtcgt tggcttcacg cgtgacgaca ttaccttcgt cgtcctgccc
     1741 ttcgaccgga ggaatatcca cgggcgaagg caagtagtcg acgacggcgt caagcatgcg
     1801 ctgcacgccc ttgttcttga aagcggtgcc gcaaagcata ggctggattt cgccggcgat
     1861 cgtgcgtgtg cggatcccca acgtaatctc gtcctggctt agcgaacccg tttccaggta
     1921 tttgttcatg agttcttcgg tcgattcggc cgccgactcg agcagctttt cgtgccactc
     1981 gttagcctga tcaagcaatt ccggcggaat atcgatgtat tcgaacttga tgccctgaga
     2041 agcctcgtcc cagatgattc ccttcatctt caccaggtcg acaacccccg tgaagttttc
     2101 ttcggctcca atcgggataa cgataggcac cggatgcgcc ttcaggcgcg tcttgagctg
     2161 ctcgtacacc ttgaagaaat tcgcgccggt tcgatccatt ttattgacga acgccaagcg
     2221 tggaaccccg tatttgttgg cctgacgcca gacggtctcg gactggggct gcacaccgcc
     2281 caccgcacaa tacaccatgc aagcgccatc gagcacgcgc atcgaacgtt cgacttcgat
     2341 ggtgaaatca acgtgtcccg gggtgtcgat aatgttgatg cgatgctgcg ggcggtcgcc
     2401 cgccatgccg ctccagaaag cagtagtggc cgccgacgtg attgttatgc cgcgctcttg
     2461 ctcctgctcc atccaatcca tggtggcggc gccatcatga acttcgccaa gcttgtggtt
     2521 gacgcctgta tagaacagga tgcgctcagt ggtggtggtt ttcccggcat caatatgcgc
     2581 ggaaatgcca atattgcggt agcgctcaat gggggtt
//
LOCUS       DATCXU010000102         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_812408,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000102 DATCXU010000000
VERSION     DATCXU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812408"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIR04_02220"
     CDS             <1..374
                     /locus_tag="VIR04_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY9459597.1"
                     /translation="PLAGIALNTDTSIMTAIANDYGFDTLFERQVTALGQPGDVLVAI
                     STSGNSANVLNSIAAAHEREMTVIAITGKGGGKINELLTDADIHLCVPHDRTMRIQEV
                     HIVLLHALCDGIDALLLGDNL"
     gene            374..1090
                     /locus_tag="VIR04_02225"
     CDS             374..1090
                     /locus_tag="VIR04_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY9459598.1"
                     /translation="MQASTHFRATSRSALFICLTLTILAPLSGCGLLVVGGTAATTAA
                     VATDRRTAGQQVEDKSIELKTVNEVGKLFGDNARVDATSYGGLLLLTGDVPTAQDKQK
                     AEQTAAKIEKVKKVVNELRVGDITPLSVRTNDSWITSKVRTSLINTQEVPSRTIAVTT
                     ERGIVYLLGIVTDEEGQRAAKVASNVSGVNKVVKLFQIVSPESLMQTQTPAPVENAAA
                     PAPAPAPAAPAGDIQTMPVK"
     gene            1087..1581
                     /locus_tag="VIR04_02230"
     CDS             1087..1581
                     /locus_tag="VIR04_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY9459599.1"
                     /translation="MKKTLIGFIAVIALAGAGFWQLSGPAKTAPDVTFTSLTGQKITT
                     KDLRGKVVLVKFWATSCVTCVQQMPDMIKTYDELAPKGYQAIAVAMQYDPPNYVLNYA
                     QTRHLPFTVAIDAQGEIAKAFGDIKLTPTAFLIDKKGHIIKRYLGNYDKVAFLNTVEK
                     ALAG"
     gene            1785..2690
                     /gene="argB"
                     /locus_tag="VIR04_02235"
     CDS             1785..2690
                     /gene="argB"
                     /locus_tag="VIR04_02235"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY9459600.1"
                     /translation="MTLSPPSDSANHSSTVKADVLSEALPYIRRFHGKTVVIKYGGNA
                     MTEEHLQRSFAHDVVLLKLVGLNPVVVHGGGPQINQALKRIGKEGTFIQGMRVTDAET
                     MEVVEWVLGGQVQQDIVMMINEAGGKAVGLTGKDGCLIQATKKMLVDKENPGQLIDIG
                     YVGDIARIEPAVVKALQDDQFIPVISSIGYGEDGTAYNINADLVAGKMAEILGAEKLV
                     MMTNTPGVLNKDGQLLRSLSAQTVDELFEDGTISGGMLPKLASALEAARNGVTSVHIV
                     DGRVPHCLLLEILTDRGVGTMISSH"
     gene            2704..>3059
                     /locus_tag="VIR04_02240"
     CDS             2704..>3059
                     /locus_tag="VIR04_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY9459601.1"
                     /translation="MRRPAAPHRLPLHRVRTGPDDPVWLFDLDNTLHDSSREIFKAID
                     VAMTAAVATSLNIDSDAASQLRTLYWRRYGATVIGMVNHHGIDAKTFLDLSHRFDVAP
                     LVHGEPGLAYRLRRLKG"
BASE COUNT          720 a          908 c          828 g          603 t
ORIGIN      
        1 tgccgctggc cggtattgcg ttaaataccg acacgtcgat catgactgcc atcgccaacg
       61 attacggatt tgacaccctc ttcgagcggc aggtaacggc actggggcaa ccgggcgacg
      121 ttctggtcgc catttccacc agcggaaact cggccaacgt tttaaactcc attgccgccg
      181 cgcacgaacg cgaaatgacc gttatcgcca ttaccggcaa aggcggcggc aagatcaacg
      241 aattactgac cgacgccgac attcatctgt gcgttcccca tgaccgcaca atgcgcattc
      301 aagaggtgca tatcgtgctt cttcatgcgc tttgcgacgg tatcgatgcc ttgcttctgg
      361 gagacaatct gtaatgcaag caagtaccca tttccgcgcc acatcacgct cagcactgtt
      421 tatttgtctg acgctgacca tcttggcccc tttgtcgggc tgcggcctgc tggtcgtcgg
      481 gggcaccgcc gccaccacgg ccgcggtggc cactgaccgg cgcacggcgg gccagcaagt
      541 cgaagacaag tccatcgagc ttaaaacggt caacgaggtg ggcaagcttt tcggcgacaa
      601 cgcccgggtc gacgcgacct cgtacggcgg cctgttactc cttaccggcg acgtccccac
      661 cgcgcaagac aagcagaaag cggagcaaac agccgccaaa atcgaaaagg tgaaaaaggt
      721 cgtcaatgaa ctgcgcgtcg gagacattac ccctcttagt gtgcgcacca acgattcctg
      781 gattacctcg aaggtcagaa cgtccctgat caacacacag gaagtgccca gccgcaccat
      841 cgccgttacc actgaacgcg gaatcgtcta tttgcttggc atcgtcaccg acgaagaagg
      901 acagcgggcc gccaaagtgg catccaacgt cagcggcgtc aacaaagtag taaaattatt
      961 ccagatcgta tcgcccgaaa gtctgatgca gacgcaaact ccggcgccag tcgaaaacgc
     1021 cgcggcgcct gccccggctc cggcccccgc cgctccagcc ggcgatatcc aaacaatgcc
     1081 cgtcaaatga aaaaaacgct tattggcttt attgccgtca ttgccctggc aggcgccggt
     1141 ttctggcaac tgtccgggcc ggccaaaact gccccggatg tcacatttac atcgctgaca
     1201 ggccagaaaa taaccacgaa ggacttgcgc ggcaaagtcg tactggtcaa gttctgggcg
     1261 acaagctgtg tgacatgcgt gcagcaaatg cccgacatga tcaaaaccta cgacgagctc
     1321 gcccccaaag gctatcaggc catcgcggtc gcaatgcaat atgatccgcc caactatgtg
     1381 ctgaactatg cccaaacgcg ccaccttccc tttaccgtcg cgatcgacgc ccaaggggaa
     1441 atcgccaagg catttggcga tatcaagctt acgccgaccg cttttcttat tgacaagaag
     1501 gggcacatta tcaagcgcta cctgggcaac tacgacaagg tggccttttt aaatacagtg
     1561 gaaaaggccc tggccggctg acaccagcac acatcattcc gcttccaaat cattcgtgaa
     1621 cgcgaaagca aagcgtagac agtacaagcc gtacggcaag cgaagctgac aaagtgcacg
     1681 aatgatttgg aagcggaata agtccctggc cccgacacaa cccgagtacg cctgagcgcg
     1741 cctcgggttt ttcacaacca ccctttcttt ttttcagtgt tgccatgacc ctatcgcctc
     1801 cctctgattc cgccaaccac tcgtcgaccg tgaaagccga cgttctgtcc gaggcattgc
     1861 cttatatccg gcgttttcac ggcaagaccg tcgtcatcaa gtacggcggc aacgccatga
     1921 cggaagaaca cttgcagcgc agtttcgccc acgatgtcgt tttgttgaaa ctggtgggac
     1981 tgaacccggt ggtggtgcac ggcgggggcc cccagatcaa tcaggcgcta aagcggatag
     2041 gcaaagaggg aacctttatt cagggcatgc gtgtaaccga tgccgaaacc atggaagtgg
     2101 ttgaatgggt actgggcggc caggttcagc aagacattgt catgatgatc aacgaagccg
     2161 gcggcaaagc tgtcggcctt accggcaaag acggctgcct gattcaggca acgaaaaaga
     2221 tgctggtcga taaagaaaac ccgggccaat tgatcgatat cggctatgtt ggcgatatcg
     2281 cgcgcatcga accggcggtg gtaaaggcct tgcaagatga tcaattcatc cccgtcattt
     2341 cttcgatcgg ttacggcgaa gatggcacgg cttataacat taacgctgat cttgtcgcgg
     2401 gaaaaatggc cgaaatactg ggcgctgaaa aactggtcat gatgaccaac accccgggtg
     2461 tgcttaataa agacggccag ttgctgcgca gcctttccgc gcagaccgtc gacgaacttt
     2521 ttgaagatgg cacgatttca ggcggcatgc tgcccaagct ggcgtccgcg ctcgaggccg
     2581 cccgcaacgg ggtaacgtcc gtgcacattg tcgatggacg cgtgccccat tgtcttttgc
     2641 tcgaaattct gactgatcgc ggcgtcggaa ccatgatcag ttcacattaa accgatgcgc
     2701 gttttgcgcc gccccgcggc gcctcatcgc ttgccgttgc accgggttcg gacggggccc
     2761 gatgacccgg tgtggttgtt cgatctggac aatacgctgc acgactcgtc gcgcgaaatc
     2821 ttcaaggcga tcgacgttgc catgaccgcg gccgtggcca ccagtctgaa catcgacagc
     2881 gacgcggcaa gccaattgcg caccctgtat tggcggcgat acggagcgac ggtcatcggc
     2941 atggtgaacc accacggaat cgacgccaaa acttttctgg atcttagcca tcgattcgac
     3001 gtcgcaccgc tggtgcatgg cgaacccggg ctggcctatc gattgcggcg gctgaaagg
//
LOCUS       DATCXU010000103         5766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1131261,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000103 DATCXU010000000
VERSION     DATCXU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131261"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..507)
                     /locus_tag="VIR04_02245"
     CDS             complement(106..507)
                     /locus_tag="VIR04_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9459602.1"
                     /translation="MDTKEKMVARRTPEPTIGGSFYIRAGEMEWKPTRFDKVSMKILY
                     EDKEKGEMTCMIKLDPGAYIPFHQHPELEQAYMLAGSMEDHDGVARAGDFIWRKAGSR
                     HDNTSKEGAIILAVYRKPNLYYHATGETSGF"
     gene            complement(538..1452)
                     /locus_tag="VIR04_02250"
     CDS             complement(538..1452)
                     /locus_tag="VIR04_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459603.1"
                     /translation="MRAEIVREGAVAPAAGGSGAARTQSSAALDEYRKRILRETRRDK
                     LVVFGYQVLVFAVFLALWETLTRIDWLAKNTFIDPFFISRPSLVIERLYQWTLGSDAG
                     FIWPHLWATLYATLLGLVASVATGFVVGLLLSQNPRVARVLSPFINGLNSLPRIAFVP
                     LITMIFGLGLASKVVTAWFIVFFLVFFNTFKGCCSIEKHIVDFCRTLGASPTQITWNV
                     RIPHALAWTFAVLPNAVAFSLIGVVIAEFIGSTVGMGYLIITSLSTLNSTDMFATITV
                     LSILGIVLVTAIGRVERYLMRWAPEFHD"
     gene            complement(1442..2239)
                     /locus_tag="VIR04_02255"
     CDS             complement(1442..2239)
                     /locus_tag="VIR04_02255"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459604.1"
                     /translation="MIELQGVSKVYYSKSRESYAVGDASFHVGEGEFVSIVGPSGCGK
                     STLLNMIASFMEPTSGTIEVMGKPVAAGHIPEGLGYIFQKDTVLPWFTVARNIGLGLR
                     FRGVPEPEIAAKVNYLLALANLQGCQDLFPHHLSGGMKQRVALLMTLACEPKILLLDE
                     PFGALDSNTKMVLHKELLDIWDQLGQTIVMVTHDLDEAVTLSDRVIVLSAPPSRVVLD
                     YRINLERPRDVFSLRQSREFGDHVREIWGVLGREFQKARQEALDHAS"
     gene            complement(2236..3300)
                     /locus_tag="VIR04_02260"
     CDS             complement(2236..3300)
                     /locus_tag="VIR04_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459605.1"
                     /translation="MIVKSKVSENTSRRLFLRGAVGLSIGGLVIPSWGFAQAKNAGTA
                     TYGVASIDPSYALIYVALKKGYFTDAGVKVEYLNSQSGPRTKQMVAAGQLLMGTSGVS
                     DAIALTIAGKQCVLVGCLDTRVAYANILINKKLHDKGIKDVKQLDGLSIGVTQPQAGT
                     WLMATYIIDRAGLKGKVTIKPLGDYATMMGAVKSGAVDCTMATFGMLEKGQEEGWGAT
                     LFDTTNAETWNAIFGGDVPGYGCYVMQEQIEKRPDTVQAMMTGLSKASDFLKQASPAE
                     IADTVYADYLKGFSMQSVVSAITVYKKSWSYDNLITEANYARLISIMSGRQYSKEDLA
                     KAPYEKNVNMSFLQKARNKA"
     gene            3672..4736
                     /locus_tag="VIR04_02265"
     CDS             3672..4736
                     /locus_tag="VIR04_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459606.1"
                     /translation="MIIKSKLSEDTSRRQFLRGALSLSIGGILLPSLSFAQAKSPGKA
                     IFGVSSIDPSYTMVYVALKKGYFHDAGVDVEYLNSQSGPRTKQMVAAGQIQMAITGVN
                     DAVALTTAGKQCVLVGCLDTRIVYGNILISKKLYDSGIKDVKQLGGHSIGITQPQGGT
                     WLMATYMTDRAGLKGKVTIKPLGDYATMMGAVKSGSVDCTMATFGMLDEGQKQGWGAT
                     LFDVNDTKTWNDIFGGDIPGNGLFVMQELIDQRPETVQALVTALTRATDFLKGSSPEE
                     IAEVVYADYLKGFSKPAVISAITTCKKTWSYDNVISKDSYERLISMMTGRQYSKEQLD
                     KDPYEKQVNMSFVKKARKMA"
     gene            complement(4786..>5766)
                     /locus_tag="VIR04_02270"
     CDS             complement(4786..>5766)
                     /locus_tag="VIR04_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY9459607.1"
                     /translation="EGISEAGAISSWMAAATSYSVHGVAMLPFYIYYSMFGFQRVGDL
                     IWAAADQRPRGFLIGATSGRTTLGGEGLQHQDGNSHVIAAMVPNCRAYDPAYAYELAV
                     ILDHGMRQMLELQRNEFYYITTLNENYAQPSMPEGVEADIVRGMYCVGARQPAGRSRS
                     AKHPTVRLLGSGAILREVIAAADLLALDWNVGSTLYSVTSYGELAREAREAERHNRLH
                     PGEPIVGSHVQACLQGDDPIIAASDYVRAYPGLIAPYVDSRFITLGTDGFGRSDTREE
                     LRSFFEVDRHHIALAALDALAQAGRIKREKVAAAIQLYGINPDRTASWNG"
BASE COUNT         1400 a         1651 c         1487 g         1228 t
ORIGIN      
        1 cctgccagcc gtctttcaat agcgccaacg atgccgcctt gccaatgccg gtgccagcgc
       61 ccgtgacaat caccacctta tggctggaat cgataacgga acccattaaa aaccgctcgt
      121 ttcgccggtg gcgtggtaat aaagattagg ctttctgtaa accgccaata tgatggcccc
      181 ctctttcgag gtgttatcgt ggcgcgaacc cgctttgcgc cagatgaaat caccggcgcg
      241 cgcgaccccg tcatgatcct ccatagagcc cgccagcatg tacgcctgtt ccagttcggg
      301 atgctgatga aaagggatat aggcgcccgg atcgagcttg atcatgcaag tcatctcgcc
      361 cttttccttg tcttcgtaga gaatcttcat tgatacttta tcaaaacgag tgggcttcca
      421 ttccatttca ccggccctga tgtaaaaaga cccgccaatg gtcggctcgg gtgtgcggcg
      481 agcaaccatc ttttctttcg tatccataca acctccaaaa taaatcagcc cgcaacatca
      541 gtcatgaaac tcgggcgccc agcgcatcaa gtagcgctca acacggccaa tggccgttac
      601 caaaacaatg cccaatatcg agagcaccgt aatcgttgca aacatatcgg tcgaattcaa
      661 ggtcgacaag gaagtaatga tcagatagcc cattccgacc gtggatccga tgaactcggc
      721 aatcacgacc cctatcagcg aaaatgccac tgcgttggga agcaccgcga atgtccatgc
      781 caaggcatgc ggtatgcgaa cattccaggt gatttgcgtg gggcttgcgc ccaacgtacg
      841 acagaaatca acaatatgct tttcgatgct gcaacacccc ttgaaggtgt tgaaaaaaac
      901 cagaaaaaat acgatgaacc aggcggtgac gaccttcgat gccagaccta acccaaagat
      961 catggtgatg agcggcacga acgcgatgcg cggcaaagag ttcaatccat taatgaaggg
     1021 agacaacacc cttgccaccc gcggattttg cgacagcagc aaccccacca caaaaccggt
     1081 ggccaccgac gccaccaggc ccagcaaagt ggcgtataaa gtggcccaca aatgcggcca
     1141 gataaacccc gcatcgctac ccagtgtcca ctggtacagt cgctcgatga ccagcgacgg
     1201 tcgacttatg aaaaagggat caatgaatgt attcttggca agccagtcaa tgcgggtaag
     1261 agtttcccaa agcgccagga aaacggcaaa aaccagaacc tgataaccga acacaacaag
     1321 tttgtcgcgg cgtgtttccc tgaggattct tttccgatat tcatccagcg ccgccgaact
     1381 ttgagttcgc gccgcgccgc tgccgccggc ggccggtgcg acagcgcctt cgcgtacgat
     1441 ttcagctcgc atgatcaagt gcctcttgtc tggctttctg gaattcacga cccagcacgc
     1501 cccatatttc acgcacgtgg tcgccaaatt cgcgcgactg ccgcaatgag aatacatcgc
     1561 gcggccgttc cagattgatt ctgtaatcca gcaccacgcg gctgggtggc gcggacaata
     1621 cgatcacacg atccgaaagc gtgacagcct cgtccagatc gtgtgtaacc ataacgatcg
     1681 tctgaccgag ctgatcccag atatcgagca gctccttgtg caaaaccatt ttggtattgc
     1741 tgtccagcgc gccgaacggc tcatccagaa gcaatatttt cggttcacag gccagcgtca
     1801 tcaacaaggc gacgcgctgc ttcatgccgc cggacagatg atggggaaac agatcctggc
     1861 aaccctgaag attggccagg gcaagaaggt aattgacctt ggcggcgatc tcgggttcgg
     1921 gcacaccgcg aaaccgcagc cccaggccaa tattgcgcgc aaccgtaaac cagggcagca
     1981 ccgtatcttt ttgaaagatg taccccaggc cctcgggtat gtgccccgcc gcaaccggct
     2041 ttcccatgac ctctatagtg cccgaggtcg gctccataaa gctggcaatc atattgagga
     2101 gggtgctttt gccgcaaccg ctgggcccca caattgacac aaattcgccc tcgccaacat
     2161 ggaagctggc gtcgccaaca gcgtaacttt cacggctctt ggaataatag actttggata
     2221 caccttgcag ctcgatcatg ctttattcct tgccttttga agaaagctca tgttcacatt
     2281 cttttcatat ggagcttttg caagatcttc tttactatat tgacgccccg acatgatgga
     2341 tatcaagcgg gcatagttgg cttcggtgat cagattgtca tagctccagg atttcttgta
     2401 aacagtgatc gcgctgacaa ccgactgcat cgaaaaacct ttcaggtagt cggcataaac
     2461 ggtgtccgcg atttcggccg ggctggcctg cttgaggaaa tcgcttgcct ttgacagccc
     2521 cgtcatcatt gcctgcacgg tgtcgggccg cttttcgatc tgctcctgca taacgtagca
     2581 gccataaccg ggaacgtctc cgccaaatat ggcattccag gtctcggcat tcgttgtgtc
     2641 gaaaagcgtg gcgccccaac cctcttcctg gcctttttcc agcatgccga aggtggccat
     2701 ggtgcagtcc acggcgccgc ttttaacggc gcccatcatt gttgcgtagt ctcccaaagg
     2761 cttgatggtg accttgcctt tcaggccggc cctgtcaatg atgtaggtgg ccatcagcca
     2821 ggtgcccgcc tgcggctgag tgacgccaat cgacagcccg tcgagctgct tcacgtcttt
     2881 aatgcctttg tcatgcaact ttttattaat caggatattg gcgtacgcca cccgggtatc
     2941 gaggcatccg accagcacgc actgtttgcc cgcgatcgtc agcgcaatgg catcgcttac
     3001 gccggatgtg cccatcagaa gttgtccggc ggccaccatt tgcttggtgc gcggtccgct
     3061 ttgggaattc agatactcga ctttcacccc cgcatcggtg aaatagcctt ttttaagagc
     3121 gacataaatc agtgcatagc tgggatcgat gctggccacg ccataggtag ccgtgccggc
     3181 gttttttgcc tgcgcaaacc cccatgacgg gatgacaaga ccaccgatgg acaggccaac
     3241 agcgccgcgc agaaacaaac ggcgagaggt attttcagaa accttgcttt taacgatcat
     3301 gagcagtccc cttgcaggaa agtcttggtc cgaatatcta gcctaacatt taatgcccgt
     3361 aatttacata attatttaaa cggcggaatt gcttctgcgc gcatcgcgcc aaacacctcc
     3421 acgccgataa gatactgccg acaagctggc ataggtaact gaattgacct ttccccgttt
     3481 tacaaatcgg tatgaaatag ggaaataaag tcgaaagcag cataaaaatc ctctgtcccg
     3541 gcaaccggca ctgttcctcc acgtaaaccc tgacggaatt cttaatctat cgagagtatc
     3601 ttttgccttg ccgcgccacg tcgtttgcat tctcgataat ccttccaaca agatttttct
     3661 caaggactac catgattatt aaaagcaagc tttcagaaga tacgtcccgc cgtcaattcc
     3721 ttcgcggcgc gctgagttta tccatcggcg gaatcctgct tccgtccctt tcgtttgcgc
     3781 aggctaaaag tccgggcaaa gccatattcg gcgtttccag catcgaccca agctatacga
     3841 tggtttacgt cgctcttaaa aagggatact tccacgacgc aggagtggat gttgaatatc
     3901 tgaattccca aagcggaccg cgcaccaagc aaatggtggc cgcggggcaa atacagatgg
     3961 ccattacagg cgtcaacgac gccgtcgcgc taaccacagc gggcaaacag tgcgtgctgg
     4021 tcggatgtct cgatacccgc atcgtatatg gcaatatcct gatcagcaaa aaactctatg
     4081 acagcggcat caaagacgta aagcaactgg gaggccattc aataggcatt acccagcccc
     4141 agggcggaac gtggctgatg gccacgtaca tgacagacag ggccggactg aaaggcaaag
     4201 tcaccatcaa gcctttgggg gactatgcga cgatgatggg cgccgttaaa agtggatccg
     4261 tcgattgcac catggcgacc ttcggcatgc tggacgaggg acaaaaacaa ggctggggcg
     4321 caacgctgtt tgacgtcaac gacaccaaaa catggaacga tatatttggc ggggatatcc
     4381 cgggcaacgg cctgttcgtc atgcaggaac tgatcgacca gcgacccgaa accgtgcagg
     4441 cgctggtgac agcgttaacg cgggccaccg attttcttaa aggttccagt cccgaagaga
     4501 ttgccgaagt ggtctatgcc gattacctga aggggttttc aaaacccgcg gtaattagcg
     4561 ctataacgac gtgcaaaaaa acctggagct acgacaacgt aatcagcaaa gacagctatg
     4621 aacggctgat ctcgatgatg acggggcgcc aatacagcaa agagcagctc gacaaggatc
     4681 cgtacgaaaa acaagtgaac atgagcttcg ttaaaaaggc cagaaagatg gcctgaatta
     4741 ccgtaaccac ttcagcaacg ccctccttcc cccgaccggg atgcttcatc cattccagga
     4801 agccgtccgg tcggggttga tgccgtaaag ctgaatggcc gccgccactt tttcgcgctt
     4861 tatgcggccg gcttgcgcca gtgcatcaag cgccgccaac gcgatatgat gacggtcgac
     4921 ctcgaagaag ctgcgcagct cttcccgcgt atcgctacgg ccgaagccgt cagtgcccaa
     4981 tgtaatgaat ctggaatcga cataaggagc aatcaggccc ggataggcgc gcacgtagtc
     5041 gctggcggca atgatcggat cgtcgccttg aaggcacgcc tgcacatggc tgccaacaat
     5101 aggttcaccg ggatgaagcc ggttgtggcg ttcggcttcg cgtgcctcgc gcgcgagctc
     5161 gccatagctg gtaacgctat acagtgtgct gcccacattc cagtccagcg ccagcagatc
     5221 ggcggcggca atgacctcgc gcaagatggc tcccgatccg agcaggcgaa cggtgggatg
     5281 ctttgcggat ctggaacgac cggccggctg tcgcgcgccc acacaataca tgcctctgac
     5341 gatgtcggcc tcaacgccct cgggcattga aggctgcgcg tagttttcgt tcagcgtcgt
     5401 aatgtaatag aactcgttgc gctgcagttc gagcatctgg cgcatgccat gatcgagtat
     5461 taccgccaat tcataggcgt acgcgggatc gtaggcccgg caattgggaa ccattgcggc
     5521 aataacgtga ctgtttccat cctggtgttg caggccctcg ccgcccaggg tggtgcgtcc
     5581 cgatgtggct ccgatcagaa agccccgcgg ccgctgatcg gcggccgccc agatcagatc
     5641 gccgacgcgc tgaaaaccga acattgaata atagatataa aacggcagca tggccacgcc
     5701 gtgtacgctg taactggtgg cggcagccat ccagctagag atcgcgccgg cttcgctgat
     5761 gccctc
//
LOCUS       DATCXU010000104         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_976742,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000104 DATCXU010000000
VERSION     DATCXU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976742"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..748
                     /gene="frr"
                     /locus_tag="VIR04_02275"
     CDS             191..748
                     /gene="frr"
                     /locus_tag="VIR04_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY9459608.1"
                     /translation="MTTHDLNEIKRRMQGASAVLKTELSGLRTGRASAHLLDPVMVDA
                     YGAQMPLNQVATVSVPEPRLISVNVWDRSLVHPVEKAIINSNLGLSPATEGQTIRLRI
                     PELNEERRKELVKLAHKYAETARVAVRHVRRDAMDVIKKLEKDHKISKDDHERFSTEI
                     QKATDAGIAEIDHMLAGKEKEILTV"
     gene            775..1512
                     /locus_tag="VIR04_02280"
     CDS             775..1512
                     /locus_tag="VIR04_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY9459609.1"
                     /translation="MHPDATAFDMPRHVAIIMDGNGRWAATRGLPRAEGHRRGVEALR
                     RTVRAAGEMGIKILTIFSFSAENWSRPASEIGELMGLLRRFVRNDLAELHQNNIRVRV
                     IGERVGLDPDIGRLLVEAEELTKNNDGLILVVAFNYGARQEIVRAARRIAEAVERGEA
                     RAQDLDMDAFGRFLDAPDIPDPDLIIRTSGEQRLSNFLLWQAAYSELVFVPTYWPDFD
                     RAALESAIREYQQRERRFGGLVAQTGS"
     gene            1516..>2249
                     /locus_tag="VIR04_02285"
     CDS             1516..>2249
                     /locus_tag="VIR04_02285"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY9459610.1"
                     /translation="MAGSEPISNVKAGVPLSANNLLLRVISAAVLAPLALVAAYIGGL
                     PFALFWGVAALVVLWEWMTLVVGPNYRLLVFSCAAAIAIAGLLAWLGRPVTALFVVGL
                     GALAGAIFAPGERRLWVIAGAGYAGGMVLAPVFLRADVGFGFMAILLLFAIVWTTDVL
                     GYFAGRAFGGPKLWPAVSPKKTWSGAICGTVGAIAIALFVAAQFGSFDRIAVIAVALL
                     LSVVAQAGDLFESWVKRRFDAKDSSH"
BASE COUNT          431 a          616 c          722 g          480 t
ORIGIN      
        1 gaaaaccgta tgcctatcat cgtattctcg atcgcccaga agggtgccat cgaagccgtc
       61 ctgcgtggca aaggtcatgc gacggttgtc gggtagtttg ctgatcgcgc cgatttgagc
      121 ctttggaaag ggtgctgacg cagctgcttt gaaaaaaagg gaggcagttc cgggggtctc
      181 aatgacaaac atgacgacgc acgacctcaa cgagatcaag cgacgcatgc agggcgcaag
      241 cgctgtgctg aaaacggaat tgtccggctt gcggaccggc cgcgcatccg cgcatctcct
      301 cgatcctgtg atggtcgacg cctacggcgc gcaaatgccg ctcaatcagg tggcgactgt
      361 tagcgtgccg gagccacggc tcatttcggt gaatgtgtgg gaccgctcac tggtccaccc
      421 ggtggaaaag gccattataa attccaatct cggactttca cccgcgacgg aaggccagac
      481 gatccgcctg cgcattcccg agctcaacga ggaacgccgc aaggagttgg tgaagttggc
      541 gcacaaatac gcggagacgg cgcgtgtggc cgtgcgtcat gtccggcgcg acgccatgga
      601 tgtcatcaaa aagctggaga aagatcacaa gatcagcaaa gacgatcacg agcgcttttc
      661 tactgaaata cagaaggcga ccgacgccgg catcgcggaa atcgaccaca tgctggctgg
      721 aaaggaaaag gagatcctca cggtctgagg tcgcgttgca tgcccgagac tgaaatgcat
      781 ccggatgcaa cggcattcga tatgccgcgc catgttgcca ttataatgga cggcaacggc
      841 cgttgggcgg cgacacgtgg actgccgcgt gccgaaggcc atcgccgcgg cgtggaagcg
      901 cttcggcgta ccgtgcgcgc cgccggtgaa atgggaatca agatcctcac cattttttcg
      961 ttcagtgcgg aaaactggtc gcgtccggca agtgaaatcg gcgaattgat gggactgctg
     1021 cgccgtttcg tgcgcaatga tctcgccgaa ctgcatcaga acaatattcg cgtgcgggtc
     1081 atcggcgagc gtgtggggct ggaccctgac atcggtcgat tgcttgtcga agcggaagaa
     1141 ctgacgaaga ataacgacgg acttatattg gttgttgcgt tcaactacgg cgcgcgccag
     1201 gaaatcgttc gcgcagcgcg ccggatcgcg gaagcggtgg agcgcggcga ggcaagggcg
     1261 caggacctcg acatggatgc attcgggcgt tttctcgacg cacccgacat ccccgatccg
     1321 gatctgatca tccgcacgag tggcgagcaa cggctgtcga actttcttct ctggcaggcg
     1381 gcctacagcg aactggtctt tgtcccgacc tattggccgg atttcgatcg cgcggcgctt
     1441 gaatccgcga tacgcgaata tcagcaacgc gagcggcggt ttggcggcct cgttgcgcag
     1501 accggttcct gatccgtggc cggtagcgaa cccatttcca acgtgaaggc cggcgtgccg
     1561 ctatcggcga acaatctcct gctgcgcgtg atatcggcgg cggttcttgc gccactggcg
     1621 ctggttgcgg cctatatcgg aggcttgccg tttgcgctgt tctggggggt tgccgccctc
     1681 gttgtgctgt gggaatggat gacgctggtc gtcgggccga actatcggct gttggtgttc
     1741 tcgtgtgcgg ccgcgattgc aattgccggt ttgcttgcct ggctcggcag accggtaacg
     1801 gcactctttg tggttggcct gggcgctctc gccggcgcca ttttcgcacc gggcgaacgg
     1861 cgactctggg tgatagcggg tgccggttac gcaggcggca tggtcctggc gccggtgttt
     1921 ttacgggcgg acgtcggttt cggatttatg gcgatcttgc tgctctttgc gatcgtctgg
     1981 actaccgatg tcctcggcta ttttgccgga cgggctttcg gtggcccaaa actgtggccc
     2041 gccgtaagtc cgaagaaaac gtggtcaggc gcaatttgcg gtacggtcgg agcgatcgct
     2101 attgcattat ttgtcgccgc ccaatttggt tcattcgaca gaattgcggt tatcgccgtg
     2161 gcattgttac tgtccgttgt ggcccaggct ggcgacctgt ttgaatcctg ggtgaaacgg
     2221 cggttcgacg ccaaggactc aagccacat
//
LOCUS       DATCXU010000105         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_812249,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000105 DATCXU010000000
VERSION     DATCXU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812249"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1157
                     /locus_tag="VIR04_02290"
     CDS             99..1157
                     /locus_tag="VIR04_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY9459611.1"
                     /translation="MFFISLAATVALPRDRLADYPPVEPGHALRFPADHGAHPDFRTE
                     WWYVTGWLQTPAGSPLGFQVTFFRSRPRFAPGNPSRFAPKELLFAHVAISDPALGRLQ
                     HDQRVARAGFGLAQALAGNTDVHIGPWSLKRNPAGVYHAAIQARDFTFELTLRPTESL
                     LLEGDDGYSRKGPLPLQASYYYSQPQLAVSGTLVRGGQRQTVQGTAWLDHEWSSAALA
                     EQAVGWDWVGVNLADGGALMAFQIRDKTGRKFWAGGMLRRGDGSQAHLPSSAIAFTPL
                     RHWRSPRTATDYPVSMRVEAGGHTLELMPLMDDQELDSRASTGAIYWEGAVTALEGGQ
                     SVGRGYLELTGYSQRLNF"
     gene            1217..2101
                     /locus_tag="VIR04_02295"
     CDS             1217..2101
                     /locus_tag="VIR04_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9459612.1"
                     /translation="MFEGFTRTVIKTHGAEIVTVTGGEGPPLLLMHGNPFNHLSWHKV
                     APRLARDFTVVCTDLRGYGDSSKPPGGGDHSDYSFRSMAQDQVEVMQALGYTQFFAAG
                     HDRGARVLHRMCLDHEDRVLRAAFMDMLPQHYLLNNVTRQWGQFSWHWFFMIQPFPTP
                     ETMMGADPEFFIRRKLSKTDQGTSFFGTEALADYIRCIKNPDTIHAMCEDYRAAFGVD
                     LDMDTVDFDAGRRVNCPALILWGEKGGVGRNHNAAEIWARYATRIEKAASVPSGHYLQ
                     EECPDQTYATLNDFFMPR"
     gene            complement(2108..>2811)
                     /locus_tag="VIR04_02300"
     CDS             complement(2108..>2811)
                     /locus_tag="VIR04_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY9459613.1"
                     /translation="SPRDRAAWAEYSVNDPQATDRIAHYRAQNAALKALFPLARENVR
                     GVFLPRRVPWPRRVGIAAVWLSLGLSLGLAPKWVNSYFFAGQPAFAKNAAIAYAVYAP
                     ERRHPVEVAAADEKHLVSWLSKRLDRPLSIPSLREYGYSLVGGRLLPGESGPAAQFMY
                     EDKTGKRLTLYVTTALRHTAAIQMLPNINEQSTFYWISKGMGYALSGHGSDPHLRTMA
                     ADVCGALGGNPETWQ"
BASE COUNT          568 a          840 c          823 g          580 t
ORIGIN      
        1 ctgactgcgg ccgccagcgg acggcgcgca atgtcaagcg cggcagtgcg cgctgtgcgg
       61 gaggattggt gatgaagcgg ttttgcctcg ccctttccat gttttttatt tcgctggcgg
      121 cgaccgtggc gctgccgcgt gaccgcctcg ccgactatcc gcccgtggag cctggccatg
      181 cattgcgatt tccagccgac catggcgctc atcccgactt tcgcactgaa tggtggtatg
      241 tcacgggatg gttgcagaca ccagcgggat ctccgctagg ctttcaggtc acctttttcc
      301 gatctcgacc tcggttcgct ccaggcaatc caagccgctt tgcacccaaa gaactgttgt
      361 tcgcgcacgt cgcgatatcg gatccggcac tcgggcggct gcagcacgac cagcgggtcg
      421 cgcgcgccgg attcggactt gcgcaagcgc ttgccggcaa taccgatgtg catattggcc
      481 cgtggtccct gaagcgcaat cctgccggcg tctatcacgc ggctatccag gcccgcgatt
      541 ttacttttga actgacgctc aggccaaccg aatccttatt actggaaggc gatgatggct
      601 atagccgaaa agggcctctc cccttgcagg ccagctatta ctacagtcag ccgcaactcg
      661 cggtaagcgg aactcttgtg cgcggcggcc aacgccagac agttcaaggc acagcatggc
      721 tggatcacga atggtcatcg gccgctctgg cggaacaagc tgtcggctgg gactgggttg
      781 gcgtcaatct ggccgatggc ggcgccctga tggcgtttca gattcgcgac aagacgggac
      841 ggaaattttg ggcgggcggc atgctgcgac gcggcgatgg cagccaggcc cacttgccct
      901 cctcagctat tgcgttcaca ccgctgcggc actggcgctc cccgcgcacc gcgaccgatt
      961 atcctgtatc gatgcgggtc gaagccggcg ggcatacgct cgaattgatg ccgttgatgg
     1021 acgatcagga actcgatagc cgggccagca cgggcgcgat ttattgggaa ggcgccgtta
     1081 cggcgcttga aggcggccag tccgtcgggc gtggatatct ggaacttact ggatactcgc
     1141 agcggcttaa tttctaagcg ataatccagg caggcacggc aagagccgcg caaacattcg
     1201 ccacaggaac tcaactatgt tcgaaggctt caccagaaca gtcatcaaga cgcacggcgc
     1261 cgaaattgtg acagtaacag gcggtgaagg acctcccttg ctcctgatgc atggcaaccc
     1321 gttcaaccac ttgtcatggc acaaggtcgc gccgcgactg gcgcgtgact ttacggtcgt
     1381 ttgcaccgat ctgcgcggct atggcgacag ttccaaaccg cccggcggcg gcgaccattc
     1441 ggactactca tttcgatcga tggcgcaaga ccaggtcgag gtcatgcagg cactgggcta
     1501 tacgcaattc ttcgccgccg gtcacgaccg cggcgcgcgc gtactgcacc gcatgtgcct
     1561 ggaccacgaa gatcgcgtgc tgcgcgcagc tttcatggac atgttgccgc aacattatct
     1621 gttaaacaat gtgacgcggc agtgggggca attctcgtgg cattggtttt tcatgattca
     1681 gccgtttccc acaccagaga ccatgatggg ggccgacccc gaattcttta ttcgccgcaa
     1741 attgtccaaa accgatcagg gcacgagctt cttcggcacc gaggccttgg cggactacat
     1801 acgttgcatc aagaaccccg ataccattca cgccatgtgc gaagactatc gcgccgcatt
     1861 tggcgtcgat ctggatatgg acacggtcga tttcgacgcc ggacgccgcg tgaattgccc
     1921 cgcgcttatt ttgtggggcg aaaagggcgg cgtggggcgc aatcacaacg ctgccgaaat
     1981 ctgggcccgc tacgcaacgc gaattgaaaa agcagccagc gttccttccg gacactacct
     2041 tcaggaagaa tgtcccgacc aaacgtacgc cacgctcaat gatttcttta tgccgcgtta
     2101 gcaccaccta ttgccacgtt tccgggtttc cacccagcgc gccgcacaca tcggcagcca
     2161 tggttctcag atgagggtcg gatccgtggc ctgaaagcgc gtaccccatt cctttgctga
     2221 tccagtaaaa cgtgctctgc tcattgatat tgggcaacat ttgaattgcg gcggtgtgtc
     2281 tgagcgccgt ggtaacgtaa agcgttaatc gtttgccggt tttgtcttcg tacataaact
     2341 gggctgcggg accggattcg cccggcaaca ggcgtccgcc caccaatgaa tatccatatt
     2401 cgcgcagcga aggtatgctt agcggccgat caaggcgttt ggataaccaa ctgaccagat
     2461 gcttttcatc cgcggctgca acctccaccg gatgacgccg ttcgggcgcg tagacggcgt
     2521 aggcgatcgc ggcgtttttg gcaaatgccg gctgcccggc gaagaagtaa gaattgaccc
     2581 atttgggcgc caatcccaga gacagtccca aacttagcca gacggcggcg atccccaccc
     2641 gccttggcca tggcacgcgg cgtgggagaa aaacaccgcg aacattttca cgagccaagg
     2701 gaaacaacgc tttcagcgct gcattctgtg cgcggtaatg ggcgatccga tcagtggctt
     2761 gcggatcgtt gacggagtat tccgcccaag ccgcgcgatc gcgtggtgaa a
//
LOCUS       DATCXU010000106         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_261816,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000106 DATCXU010000000
VERSION     DATCXU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261816"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VIR04_02305"
     CDS             complement(<1..1065)
                     /locus_tag="VIR04_02305"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEY9459614.1"
                     /translation="MSIAQQVFLRDAMRRLNLTRDVFSARVGVKRRALDTWLLPEGSQ
                     EFRAMPEVVSRFVTEIVENESLLQKYAQSAQSGPLRDRIAGADGKHQLLSVDQFSRES
                     VEELFRIADLMQPIARRQKVSRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDT
                     TGFTFSSMAKGESIYDTSRVMSGYVDAMVIRHPEKGSVGEFARATNIPVVNGGDGPGE
                     HPSQALLDLYTILTEFSRLGKLLDGAHIALAGDLKYGRTVHSLMKLLGLYRGLKFTLI
                     SPRGLEMPDYIVEQTGKRGHSVEQTHSLEQGLAGADVIYATRVQKERFSGEEIEGYSP
                     EFQINRAIVDKCGKPDVIVMH"
     gene            complement(1160..1714)
                     /locus_tag="VIR04_02310"
     CDS             complement(1160..1714)
                     /locus_tag="VIR04_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459615.1"
                     /translation="MDVNRANQDSAFQGLTTTFALGFKFLAWINGVGALLIVACSIGV
                     VQVDLSPYLLRLPLASFMAGLALCGLGLLWSYLVQSSLFNQLLEGRARRTHWVPMFCA
                     MIAYSLSMAAFVMGCWFTLNLAAAIDQNGDYSQSSHEEASPSDQSGRFGELGPVTRKG
                     VVLRDVPAAFGIRALPVGHAVKYF"
     gene            complement(1742..2335)
                     /locus_tag="VIR04_02315"
     CDS             complement(1742..2335)
                     /locus_tag="VIR04_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459616.1"
                     /translation="MSMVTLQGLGRILVPLSVVLLAACQSVPAKKGAASTTPSAQTTL
                     QATSQGAPVAVYLADTTQRTGWTAVQIQNGSALYVNPQPVITRADLAGVKAGSNKEGQ
                     GLLALDLNDAGKQKVKDVTTKNPNKRLALVVGRTMLAAPTYSTPVSAPQLIFPVGTEA
                     NAIAAAKAIAGAGATPDAASKAPASDATAPHVPTPAH"
BASE COUNT          649 a          750 c          677 g          547 t
ORIGIN      
        1 gtgcatgaca atcacgtcgg gcttgccgca cttgtcgact atggcgcgat taatctggaa
       61 ttccggggaa tacccttcta tttcttcacc agaaaaccgc tctttttgca cccgggtggc
      121 gtaaatcaca tcggcgcctg ccagtccctg ctcaagcgag tgagtctgtt caacactatg
      181 cccgcgtttg ccggtttgtt caacaatata gtcgggcatc tccaggccgc gaggcgaaat
      241 caaggtgaat ttcaggccgc gatacaagcc cagcaacttc atcagtgaat gcaccgttct
      301 gccatatttc aaatcgcctg ccagcgcaat atgggcgccg tccagtaatt tgcccaagcg
      361 ggaaaactca gtgagtatcg tgtacaaatc gagcagtgcc tgactgggat gctcgccggg
      421 gccatcccca ccgttgacaa ctgggatatt cgtcgcgcgg gcgaactccc ccaccgaccc
      481 cttttccgga tggcgtatta ccatggcgtc gacgtaaccg ctcatgacac ggctggtgtc
      541 gtagatggat tcacctttgg ccatggacga aaaagtaaac ccggtcgtat cgcagaccga
      601 gcctccaagc cggcaaaagg cggcgccgaa actgacgcgc gtacgcgtgc tggcctcgaa
      661 gaacaaattg cccaacaccg cgccttcgag gacccgggaa actttttgac gccgcgcaat
      721 tggctgcatc aagtcggcga ttcgaaacaa ttcttcgacc gattcgcgcg aaaactgatc
      781 gaccgataaa agctggtgtt tgccgtcggc gcccgcaata cgatcccgca atggcccgga
      841 ttgcgcgctt tgcgcatact tttgcaataa cgattcgttt tcaacgattt ccgtgacaaa
      901 tcggctaacc acttcgggca tggcgcgaaa ctcttgcgac ccttcgggaa gcagccacgt
      961 atcaagcgcc cggcgcttta cgcccacacg cgcggaaaac acatcgcggg tcaaattcaa
     1021 acgacgcata gcgtcgcgca aaaatacttg ctgagcgata gacatacaaa ggacctttaa
     1081 taaaatatac gcagtgcgta cattttattc ctgcgacctt ggcttggcga gcgctattta
     1141 cgcttatttg ttcgtttttt taaaaatatt tgacagcgtg tccaaccggc aacgcgcgaa
     1201 tgccaaatgc agcgggcaca tcgcgcagga ctacgccttt tcgggtgacg gggccaagtt
     1261 cgccaaaacg accggactgg tccgaaggcg aagcttcttc gtgcgacgat tgcgaataat
     1321 ccccattctg atcaatggcg gcggccaggt tcagcgtaaa ccagcaaccc atcacaaacg
     1381 ccgccatgct taaactataa gcgatcatgg cgcagaacat tggcacccag tgcgtacgcc
     1441 gcgcacgccc ttcgagcaat tgattgaaca aactggattg caccaggtac gaccatagga
     1501 gcccaagccc gcagagcgcc aagccggcca tgaatgacgc cagcggaagc cggagcaaat
     1561 acggcgacag atcgacctga acaacgccga tcgaacaagc gacgataagc aatgcgccca
     1621 caccgtttat ccacgccagg aatttgaatc ccagcgcgaa tgtagtcgtc aagccctgga
     1681 aggcggaatc ttgattggct cggtttacgt ccatgtcttc caccaaaaag acgccaaggg
     1741 attagtgtgc cggcgtcggg acgtgcggcg cagtcgcatc agatgccggc gccttcgacg
     1801 cagcgtcggg ggtagcgcca gcaccggcaa tcgccttggc ggccgctatg gcgtttgctt
     1861 cagtgccaac ggggaagatc agttgcggcg cactaaccgg tgtcgagtaa gtcggcgctg
     1921 ccaacatggt gcggcccaca accagcgcca gtcgcttatt cggattcttc gttgtaacgt
     1981 ctttcacttt ctgcttgccc gcatcgttca ggtcaagcgc aagcaaaccc tgaccttcct
     2041 tattggaacc cgccttgacg ccagccaaat cagcgcgcgt gatcacgggt tgcgggttaa
     2101 cgtacaaggc agagccgttt tgaatctgaa ccgccgtcca gccagttcgc tgcgtcgtgt
     2161 cagccagata gaccgcaacc ggagcgccct ggctcgttgc ctgcaatgtg gtctgagccg
     2221 aaggggtcgt ggaggcagca ccttttttag ccggcacgct ttggcaagcc gccaacaaca
     2281 ccacacttag aggcacaagg atgcggccca agccttgcag agttaccatc gacatataag
     2341 attccttatg attgcccatt ttatagcatc gaaaacttta aaaacaggga aaaacggccg
     2401 agtctatttc aaaacaacgt aagccaagat atgcaacaat accagcatta aatgattaat
     2461 tcgctatgga gagccttatg gccgacgata aatacaagtt ttcagcagga aaggtagttg
     2521 caattgttac gctggtatac gccatcgcgt ttggtctgat tgtcgaccat ggaataagca
     2581 tgatcgatca tcacgcgcaa aacgcaagcc acgcatcgta aac
//
LOCUS       DATCXU010000107         5905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_103803,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000107 DATCXU010000000
VERSION     DATCXU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103803"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..997
                     /gene="accD"
                     /locus_tag="VIR04_02320"
     CDS             116..997
                     /gene="accD"
                     /locus_tag="VIR04_02320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY9459617.1"
                     /translation="MSWIEKILPPRINRNNDAGARRVPEGVWVKCLSCEAVLYKEDLV
                     ANMNVCPKCGHHMRIGARARIDSLLDVEGRTEIGETTRAVDPLKFKDSRKYTERVAEA
                     MKQTGESEAMVVMSGSILTVPVVLACFEFAFMGGSMGSVVGERFVRGVNAAIKSRTPF
                     ICVAASGGARMQESLLSLMQMAKTNAMLTRLSAAGLPFISILTDPTMGGVSAGFAFMG
                     DLVIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLSKGAIDMVLDRRQMRTELAH
                     LLALLTRQPVDVVARAT"
     gene            1080..1433
                     /gene="arsC"
                     /locus_tag="VIR04_02325"
     CDS             1080..1433
                     /gene="arsC"
                     /locus_tag="VIR04_02325"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY9459618.1"
                     /translation="MSTVTIYHNPACGTSRNTLALIRSKNIEPDIIEYMKAPLSKTKL
                     KSLISSMHISARELLRDKEAAYQDLNLADKKWSDDELVDFMIENPSLMNRPIVVTPTG
                     TRLCRPPETVLGLLG"
     gene            complement(1460..2995)
                     /locus_tag="VIR04_02330"
     CDS             complement(1460..2995)
                     /locus_tag="VIR04_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9459619.1"
                     /translation="MEQLDLLFHGISVIMSWHSVGIMLIGILLGVLVGVLPGLGAPNG
                     VAILLPLTFSMTPVSGIIMLSCIYWGALFGGAITSILFNIPGEPSSVATTFDGYPLAQ
                     QGRAAEALTLAFTGSFVGALTGVLLITFLAPLLAKFSLRFGPPEFFAVYLLTFCSFIG
                     MNKGAALKTILSMMLGFGLATVGMDSMSGQVRLTFGSVELMQGVDFLIAVIGLFGIGE
                     ILITMEEGLEFKGKNARMSLKVILKTWAGLPKYWATLVRSACVGCWMGITPGGATAAS
                     FMSYGLAERFSHSPDKFGKGEPEGVLAPETAAHASGTSALLPMLALGIPGSATAAVLL
                     GGLMIWGLQPGPLLFIEQKDFVWGLIASMYLGNVAGLIIILATVPLFASIMRVSFSII
                     APVIIVICAIGAYTAHNSMLDVWLMALFGVVGYAFKKLNYPVAPLILALVLGDRTEDS
                     FRQSMLASKGDLSVFWSNSLVASITLLAILLLLWPLFLQIWSKRRSRTATLARKQAAA
                     KTP"
     gene            complement(3031..3552)
                     /locus_tag="VIR04_02335"
     CDS             complement(3031..3552)
                     /locus_tag="VIR04_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY9459620.1"
                     /translation="MESNQENNSANGRFKWATYATADVVVATITLLVGIAMMVDSYRI
                     GSGWSDGNLQPGYFPFRLGIIICIVSLAILAHALWGKNRNTRLFVQWGQLKRILAVFI
                     PTLVYIFATEVIGIYVASALFIGAFMRLGGKHGWLKIIAVSAGTSVVLFVLFEIVFLV
                     PLPKGPLEAFLGY"
     gene            complement(3555..4574)
                     /locus_tag="VIR04_02340"
     CDS             complement(3555..4574)
                     /locus_tag="VIR04_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY9459621.1"
                     /translation="MSRIKTYLKTGLKHSLTGLALGLLCIGGNANAAWEPTKPITFVV
                     TGGAGGGADQIARLIQGAAIKHHLTKQPMVVVIENGGGGGQGFMDIKNSTGDANKIMI
                     ALANLYSVPLSTNLPFNWRDTTPVALMAMDEFVLWVNSKSPYKTAADYINAVKAKPDT
                     FKMGGAGSKREDEIVTKMIEQATGTKFTFIPYKGGGEITAQLVGGHIDSDVNNPIEHL
                     ATWRAGEVRPLCVLDDKQMPDKDKVTDTKSWNDIPTCQSQGINTKYLMIRGIFMPKGV
                     TPDQLQYYVGLMQKVRETPEWKDFIKRGAYSDDFLTGKEFADFLAQDEKHLKDVMQAT
                     GLIAK"
     gene            4806..5585
                     /locus_tag="VIR04_02345"
     CDS             4806..5585
                     /locus_tag="VIR04_02345"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY9459622.1"
                     /translation="MDPIIRPKEAYFEQTARNMEKFLRVPDWTIQQKIALTCRILAAE
                     GHESALAGQITARGEKPGTYWMLSFGLGFDEAMASNIVLVDDDLNILEGEGMVNPSNR
                     FHLWIYRHRPNVNGIVHTHPPYVSALSLIGEPLIPAHMDTSMFYDDCAYLAEWPGAPV
                     GDDEGRLINEALGDKRAILLAHHGQLTACSTIEEACVLGVFIERAARMQILARSAGTI
                     TSIKPECAREAHDYRLKPKAVGGTFHYFARRILKKDDEALR"
     gene            complement(5679..>5905)
                     /locus_tag="VIR04_02350"
     CDS             complement(5679..>5905)
                     /locus_tag="VIR04_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459623.1"
                     /translation="AAAQTPAPAGHADTEPSNKASLRDTINAAGMQWIETKPTHPAPP
                     PSAPVHLGRARKVVSHAAAEPLKQVETHHS"
BASE COUNT         1322 a         1582 c         1644 g         1357 t
ORIGIN      
        1 agggcttgat tccaaagacg ccgatgccgc cgccattgcc gcagccggca cctggttggg
       61 cgccatacgg cgcgcccttg attaatgtct tacgcttact gactggatgc gctaaatgag
      121 ttggattgaa aagattctgc cgccgcggat taatcgcaat aacgatgcgg gcgcgcgccg
      181 ggtccccgaa ggcgtttggg tcaaatgtct ttcatgcgag gcagtactgt acaaagaaga
      241 ccttgtcgcc aatatgaatg tttgcccaaa gtgcggccat catatgcgta ttggcgcgcg
      301 tgccaggatt gattctctgc ttgatgtgga agggcgcact gaaatcggtg aaactacgcg
      361 ggcagtggat ccattgaagt ttaaagattc gcgcaaatac acggaacgcg tcgccgaggc
      421 gatgaagcaa acgggcgaat ccgaagccat ggtcgtcatg agcggaagca tactgacggt
      481 gccggttgtg ttagcgtgtt tcgagttcgc attcatgggc gggtcgatgg gctcggttgt
      541 cggcgagcga tttgtccggg gtgtgaatgc ggcgattaaa agccgaacgc cgtttatatg
      601 cgttgcggct tcgggcggtg cgcgcatgca ggaaagcctg ctttcattga tgcaaatggc
      661 caagaccaat gccatgctga cccggctgtc ggccgccggc ttgccgttca tcagcatcct
      721 gaccgacccc accatgggcg gtgtttcggc tggcttcgcg tttatgggcg atctcgtcat
      781 tgccgagcca aaggcattaa ttggtttcgc cgggccgcgc gtcattgaac agaccgtgcg
      841 cgaaaaattg cctgaaggct ttcaacgagc ggaatttctg ctgtccaaag gcgctatcga
      901 tatggtgctg gatcggcgtc aaatgcgcac cgagctggcg catttgctgg ccctgcttac
      961 gcgccagccg gttgacgtcg tggccagggc gacttgatgt ttgcttgacc ttgcgggtgc
     1021 gcggcgttaa aacaaacggc ccttattgca aaacacctac taaatgtgga aatagaacta
     1081 tgtcgacagt tacgatttac cataatcctg cgtgcggcac gtctcgcaat acccttgctc
     1141 ttattcgcag caaaaacata gagcccgata ttatcgaata catgaaagcg ccgctgtcaa
     1201 aaacaaaact gaaaagcctt attagttcga tgcatatttc agcgcgtgaa ttgctgcgcg
     1261 ataaagaggc ggcctaccag gatttgaatc tcgccgacaa gaaatggtcc gatgacgaac
     1321 tggttgattt catgattgaa aaccccagcc tgatgaatcg gcccatagtg gttaccccca
     1381 ctggaactcg cctttgccga cctcccgaga ctgtactcgg tcttttagga tagtatttgc
     1441 cacgacgctg cggctagcgc taaggcgtct tggcggctgc ctgtttcctt gccagcgtcg
     1501 ccgtccggct gcgtcttttt gaccatattt gtaaaaacaa cggccacagc agcagcaaaa
     1561 tggcaagcag cgtgatgctt gcgactaaag aattcgacca aaatacgctc aagtcgcctt
     1621 tggacgcaag catcgactga cgaaacgagt cctcggtccg gtcgcccaac accagcgcca
     1681 atataagcgg tgcgaccggg taattcagtt tcttgaatgc gtatccgacg acgccaaata
     1741 gcgccataag ccagacatcg agcatcgagt tgtgcgccgt ataggcaccg atagcgcaga
     1801 tgacgataat cacgggcgcg atgatggaaa acgaaacacg catgatcgac gcgaacaacg
     1861 ggacggtcgc caggattatg attagtcccg ccacgttgcc cagatacatg ctggcaatca
     1921 atccccacac gaaatctttt tgctctatga aaagcaatgg acctggttgc aggccccaga
     1981 tcattagccc gcccagcaat acagccgccg tcgccgagcc gggaatgccc agggccagca
     2041 tgggaagcag tgcgcttgtg cccgaagcgt gagctgcggt ctccggagcc agcacgcctt
     2101 caggctcgcc tttgccgaat ttgtccggag aatgagaaaa tcgttcggcc agtccatagc
     2161 tcatgaacga ggcggccgtg gcgccccccg gggtaatgcc catccagcaa ccaacgcagg
     2221 cgctgcgcac gagtgtcgcc caatatttgg gtaatccggc ccacgtcttc aggatcactt
     2281 taaggctcat tctggcgttt ttgcctttga attccaagcc ttcttccatg gtgatcagaa
     2341 tttcgccaat gccaaagagc ccgatgaccg caatcaggaa gtcaacgcct tgcatcagtt
     2401 ccacggaacc gaaagtcagg cgcacctggc cgctcatgct gtccatgccg accgtcgcca
     2461 agccgaaacc gagcatcatg gacaggatgg ttttaagcgc cgcgcctttg ttcatgccga
     2521 tgaagctaca aaatgtcagc agataaacgg cgaagaattc gggaggtccg aatcgtagtg
     2581 agaatttcgc cagcagcggc gccagaaagg tgattaataa aacgcccgtc agggctccca
     2641 caaacgaccc ggtgaatgcc agggttagcg cctcggccgc ccggccttgt tgggccagag
     2701 gatatccgtc gaacgtggtg gccaccgatg agggctcgcc gggtatgttg aaaaggatag
     2761 aggtgatggc gccgccaaat agcgctcccc agtaaatgca ggacagcatg atgatgcccg
     2821 agacaggcgt catgctgaaa gttagcggca acaagattgc caccccattg ggtgctccca
     2881 gaccgggaag cacgccaacc agcacaccca gcaaaatccc gatgagcatt atgccgacgc
     2941 tgtgccagct catgattacc gagatgccat gaaaaagtag atctagttgt tccaatcttg
     3001 ttgctccatc gggtgtcgac cggttttgtt ttaatagccc agaaatgcct cgagcggccc
     3061 tttgggcagc ggcaccagaa atacgatttc aaataacacg aaaagaacca cgcttgtgcc
     3121 ggcgctgacc gcaattatct tgagccagcc gtgtttgccg cccaggcgca tgaatgcgcc
     3181 gataaacagg gcggatgcga catagatccc gatgacttca gtcgcaaata tgtacaccag
     3241 tgtgggaatg aaaacggcaa gaatccgctt cagttggccc cactgcacga agaggcgggt
     3301 attccgattt ttgccccata gcgcatgcgc aaggatggcc aggctgacga tgcagataat
     3361 tatgccaagc cgaaacggga agtagcccgg ctgcaggttg ccgtcggacc atcccgaccc
     3421 gattcgatag ctgtcgacca tcatggcgat gcccacgagc agggtgattg tcgcgacgac
     3481 cacatcagcc gtcgcgtatg tggcccattt gaagcgtccg ttcgcgctat tgttttcctg
     3541 gtttgattcc atgattattt ggcgatcagt ccagtggctt gcatgacgtc tttcagatgt
     3601 ttttcatcct gcgcaagaaa gtcggcaaat tccttgcctg tcagaaagtc gtcgctgtaa
     3661 gctccgcgct tgatgaagtc tttccattcg ggggtttcgc gaaccttttg cataaggccg
     3721 acatagtact gaagttgatc gggcgtaacg cctttaggca taaagatgcc tcgtatcatc
     3781 aggtatttgg tgttgatgcc ttgcgactgg caggtcggga tgtcgttcca cgactttgtg
     3841 tcggtcactt tatccttgtc cggcatttgc ttgtcgtcca gtacgcatag cgggcgcact
     3901 tcgcctgcac gccatgtggc caaatgttca atggggttgt tgacgtcgga atcgatatgc
     3961 ccgccgacca gttgcgccgt gatttcgccg ccgcctttgt aagggatgaa ggtgaatttc
     4021 gtgccagtcg cctgttcgat cattttggtg acaatttcgt cttcgcgctt cgaacctgcg
     4081 ccgcccattt tgaacgtgtc gggcttggcc ttgaccgcgt taatgtaatc cgcagcggtt
     4141 ttgtaaggtg atttggagtt gacccacagc acgaactcgt ccatggccat caacgccaca
     4201 ggagttgtgt cgcgccagtt gaacggcagg tttgtcgaca aaggcacgct gtacagattg
     4261 gccagggcga tcattatttt attggcgtca cccgtgctgt tcttgatgtc cataaagcct
     4321 tgtccaccac cgcccccgtt ttcaatgact accaccatgg gttgtttcgt aaggtggtgc
     4381 ttgatggcgg cgccttgtat cagacgtgct atttgatcgg ccccgccgcc cgcgccgccc
     4441 gtaacgacaa acgtgatggg ctttgtgggt tcccatgcgg cattcgcgtt cccgccaatg
     4501 catagcaaac ccagcgcaag gcctgtaaga ctatgcttta atccggtttt taagtatgtt
     4561 ttgatccggc tcattcgtac tcctcctcat taatggtggt atacaaagtg ccatcctaga
     4621 gacgtctgcc cctacatgca agaatttttt ttgctgcatc gcaataacaa agaacgctgc
     4681 aaggctgggc agacgcagtg actgcgggcc tgtcagccgg ctgcaattgg catcgtaaaa
     4741 agtttatatt catatcgcca atatctcctt ataattgccg ttgtatacca actaggagac
     4801 gtcgcatgga tccgatcatt cgccccaaag aggcttattt cgaacagacc gcacgcaata
     4861 tggaaaaatt cctgcgtgtc ccggactgga ctattcagca aaaaatcgcg ctaacctgcc
     4921 gcatcttggc ggcggagggt catgaatccg cgttggcggg gcaaatcacc gcccggggtg
     4981 aaaagccggg cacctactgg atgctcagtt ttggcctggg tttcgatgaa gccatggcca
     5041 gcaacatcgt gctggtcgat gacgatctta atattctgga aggcgagggc atggtcaatc
     5101 cgtccaatcg cttccattta tggatttatc gccatcgtcc aaacgtaaac ggcattgtgc
     5161 acacgcatcc gccatacgtt tcagcgttgt cgctgatcgg cgaaccgctg attccggctc
     5221 atatggacac cagcatgttc tacgacgatt gcgcttattt ggcagaatgg cccggcgcgc
     5281 cggttggcga cgacgagggc cggctgatca acgaggcgct cggagacaaa cgcgctattt
     5341 tgcttgcgca ccatggtcag ttgacagcct gttcaacgat tgaagaggca tgcgttctgg
     5401 gcgtctttat tgaaagagcg gcccgcatgc agatattggc gcgttcggcg ggaacgatta
     5461 cgtcgatcaa gcccgaatgc gcgcgcgagg cccacgatta ccgccttaaa cccaaggctg
     5521 tcggaggcac ttttcactat ttcgcgcgcc gaattttgaa aaaggacgac gaagctctgc
     5581 gctaaggaac agatgcaggc gcccgtaaca gcctgacgtc aaaccgcccc ttctgcagtt
     5641 cacaagaagg ggcggtttgt ttttcggacg gcttgtttct acgagtgatg ggtttccact
     5701 tgttttagcg gttcggcggc tgcatggctg accaccttgc gcgcccggcc cagatgaact
     5761 ggtgcggagg gcggtggcgc agggtgagtc ggtttagttt caatccattg catgcctgcg
     5821 gcattgatgg tgtcgcgcaa agaggctttg tttgacggtt ctgtatcagc atggccggcc
     5881 ggggcaggtg tctgtgcagc ggcgg
//
LOCUS       DATCXU010000108         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1162170,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000108 DATCXU010000000
VERSION     DATCXU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162170"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VIR04_02355"
     CDS             complement(<1..142)
                     /locus_tag="VIR04_02355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEY9459624.1"
                     /translation="MKMDKATSSETAVFGSGCFWCAEPVFKELRGVHDVTPGYSGGHV
                     DNP"
     gene            complement(181..528)
                     /locus_tag="VIR04_02360"
     CDS             complement(181..528)
                     /locus_tag="VIR04_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9459625.1"
                     /translation="MSLKLLRLILLVGGTVWLAPACAQSVGLGLAQSGTCMACHQVDR
                     KRVGPAFTVIAQRFSGQPGANEYLANAIRSGGRGRWGAVPMPAQPQVSPEQAKAIAAW
                     ILSLADKSTHGQQ"
     gene            591..1388
                     /gene="lgt"
                     /locus_tag="VIR04_02365"
     CDS             591..1388
                     /gene="lgt"
                     /locus_tag="VIR04_02365"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY9459626.1"
                     /translation="MLHYPQFDPIAVQIGPLAVHWYGLMYLLGFALVWVLGRWRINHG
                     KTDLSVQDLEDIIFYSVMGVVIGGRLGYVLFYKPAEYFSHPLEIFYLWEGGMSFHGGL
                     LGVIAVLTIFGYKKRKSLLEIGDFVAPLIPLGLGAGRMGNFINGELWGRPTTLPWGMI
                     FPQTQDGIPRHPSQLYEMGLEGILLFALVWWFARKPRPVGQVSAVFLMGYGLFRFLVE
                     FTRAPDYFLGLLVGGLSMGQLLSLPMMLAGALIFWASAKKSNRAPKP"
     gene            complement(1339..2076)
                     /locus_tag="VIR04_02370"
     CDS             complement(1339..2076)
                     /locus_tag="VIR04_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525304.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY9459627.1"
                     /translation="MYLSVQKRKRFFGVLAGMAASLLMVTASAHEHTEDGPAVPPQPQ
                     LTLSAQASAEVPQDTVKITLASELVGASQASVAAKLGKALDSVLKQAKTNEKVRVSSG
                     NYQVWPVNDKYGKISNWHGRGEIILESTDFVAASDLAGELSDRASIVSLRFFVSPRAR
                     AKEEQALLTQAAQAFRDRAQAATLAFGFSSYSVRNIDLNGAGAPFQPAVRMMSMATDK
                     SAAPLEGGVETVTVSVHGSIFLHSPKK"
     gene            complement(2127..2308)
                     /gene="ssrS"
                     /locus_tag="VIR04_02375"
     ncRNA           complement(2127..2308)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VIR04_02375"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2359..2670)
                     /locus_tag="VIR04_02380"
     CDS             complement(2359..2670)
                     /locus_tag="VIR04_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY9459628.1"
                     /translation="MERIDVSILGRDYSLACSPSEKEALLAAVRYVDQRMLAVKGSGK
                     VSSNERIAVMAAIQIAAEFLSMRAPGGPLSNIAVGDFKRRIDDMNSLINQVLEPAANK
                     A"
     gene            complement(2677..3183)
                     /locus_tag="VIR04_02385"
     CDS             complement(2677..3183)
                     /locus_tag="VIR04_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459629.1"
                     /translation="MIREALETGISLIEAKKHCGALNDSFISIRVSSIQPGAGWFTIA
                     PMLQDLDSLAARIGQMVQLTRELQAESTVLRARLKSAEQERGALRDQLDRRESEYQSM
                     ARRIEQHESELEAVRAQGLSAQEECATARQNLQFALAEAARLRTAAESAKDRIDMVLM
                     RLPGASAE"
     gene            3339..3800
                     /locus_tag="VIR04_02390"
     CDS             3339..3800
                     /locus_tag="VIR04_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY9459630.1"
                     /translation="MRLKNKQDFWSGIMFMAIGAGFAIGASTYSMGTSARMGPGYFPF
                     WLGICLAALGALVSLTALSKSAEHTPVEKFDWKILGIVIGSVVGCGVVLNFLGVYVAI
                     FLLVFFSSMASHIFSWKIAVINGICLMVFVWLAFIKGLGLIFPLWPSFLSI"
     gene            3814..>4730
                     /locus_tag="VIR04_02395"
     CDS             3814..>4730
                     /locus_tag="VIR04_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9459631.1"
                     /translation="MEVFDHLMLGFQVALSPENVLYCLLGCLLGPLIGVLPGIGPVPT
                     IAMLLPITYVLPPVAGLIMLAGIYYGAQYGGSTTAILVAIPGETAAVVTVLDGHQMAR
                     NGRAGAALAIAALGSFFAGCVATVIIAGFAPPLSEIAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLAKAICMILLGLLLGIVGTDVNSGVARFDFGIPELQDGIDFAAVAMGVFGFAE
                     IMSNLEMKDQRVDLTSKIGSLYPNKQEFKESWPAVVRGTALGSALGILPGGGAVLSSF
                     ASYTLEKKISRSPERFGKGHPAGLAGPESA"
BASE COUNT          973 a         1346 c         1267 g         1144 t
ORIGIN      
        1 tggggttatc gacatgaccg ccgctatacc cgggggttac atcgtgtaca ccacgcagtt
       61 ccttgaatac cggctccgca caccaaaaac aaccgcttcc gaaaactgca gtttccgaac
      121 ttgtcgcttt atccatcttc atcacctttg cactggaaac atgcgtctgt caatcttgtg
      181 tcattgttgt ccatgcgtgc ttttgtcagc caaggacaga atccacgcag caatggcctt
      241 tgcctgctcg ggactcacct gcggttgcgc cggcatggga accgcgcccc atcgtccccg
      301 gccaccgcta cgaatggcat tggccaggta ctcgtttgca cccggctgcc ccgaaaagcg
      361 ctgggctatc acagtaaacg ccggtcccac cctttttcga tccacttgat ggcaagccat
      421 gcaggtgccg ctctgagcca gaccaagtcc aacactttgt gcgcaagccg gcgcaagcca
      481 tactgtaccg ccgacaagca ggatcaatcg taaaagtttt aggctcatca atttaaacct
      541 gacattggta tcgcgttatt atcgacgaaa tttaatccgg aaggtccttc atgcttcatt
      601 atccgcagtt cgatcccatt gccgtacaga tcggcccgct cgcagtgcac tggtatgggt
      661 tgatgtattt acttgggttc gccctagtgt gggtgctggg caggtggcgc atcaatcacg
      721 ggaaaaccga cttgagcgtg caagacctgg aagatattat tttttatagt gtgatgggcg
      781 tagttattgg cggccgtctt ggttatgtgc tgttctacaa gccggccgag tatttttccc
      841 acccgctcga aattttctat ctttgggaag gcggcatgtc gtttcacggc gggctgctgg
      901 gcgtgattgc agtgctgact atctttggct acaaaaagcg caaatccctt ctggagattg
      961 gagatttcgt cgctccgctt attccgcttg ggctgggggc cggccgcatg ggcaatttta
     1021 tcaatggcga actatgggga cgacccacca ccttgccctg gggaatgatt ttcccacaga
     1081 cacaggatgg catcccgcgc cacccttcgc aattgtatga aatgggactg gaaggcattc
     1141 tgcttttcgc tctggtctgg tggtttgccc gcaagccacg ccccgtcggc caggtaagcg
     1201 ccgtatttct gatgggctac ggccttttca gattcctggt ggagttcacg cgcgcccccg
     1261 attactttct gggtctgtta gtcggcggcc tttcaatggg ccaacttctt tctttgccca
     1321 tgatgctggc cggcgccctt attttttggg cgagtgcaaa aaaatcgaac cgtgcaccga
     1381 aaccgtaacc gtctccacgc cgccttccaa cggcgccgcg gatttatcgg tcgccatgga
     1441 catcatgcgc acggccggtt gaaatggagc tcccgcgcca ttcaaatcaa tattgcgcac
     1501 gctgtaggaa gaaaaaccaa aagccagcgt tgccgcctgc gcccgatcgc ggaaagcttg
     1561 cgcggcctga gtcaataagg cttgctcttc tttcgcacga gcgcgcggtg aaacaaaaaa
     1621 tcgcaagctg actatggacg cgcgatcgct taattcgccg gccaaatccg acgcagcgac
     1681 aaaatcagtc gactccaaaa ttatttcacc gcgcccatgc cagtttgaga ttttcccgta
     1741 cttgtcgttg actggccaga cttgatagtt accgctgcta accctgactt tttcattggt
     1801 ttttgcctgc ttcaggacac tgtcgagcgc tttgcccaac tttgcggcaa ccgaggcctg
     1861 cgaggcgccg acaagctcgg atgccaatgt aattttgacg gtatcttgcg ggacctcggc
     1921 cgatgcctgc gcgctcaaag tcaattgcgg ctgtggtggc acggcgggcc cgtcttcagt
     1981 gtgttcgtgt gcggacgccg tgaccataag cagacttgcg gccataccgg ccagcacgcc
     2041 aaaaaaccgc ttgcgttttt gtacggaaag atacattgac gcccccataa aaataaaaag
     2101 cccgttgtcg ccaacgcgct ttttaaaaat gccccgcctg tgccgtctag gccggcagcc
     2161 cggaaccgag ggttcaaggt ggtcgctttc agtcaagctt cgggtttgcc aacagggcaa
     2221 tcacacctac ttgtcaatcc ggcaacctta atgccataag cattggttca aggattgtat
     2281 ggccaagtca cgaacacggc agggtctaac tggttagtcg ctttcatcaa tacacatgaa
     2341 gcgcctgata tacctacatt atgccttatt ggcggcaggt tcaagcactt gattgatcaa
     2401 actgttcata tcatcaatcc tgcgcttgaa atcccccacc gcgatgttgc ttaatggccc
     2461 cccgggcgca cgcatagata aaaattccgc agcaatctga attgccgcca tgacagcaat
     2521 acgctcgttg ctggacacct tgccagaacc tttgaccgcc agcatgcgct gatcgacgta
     2581 ccggaccgcg gccagcaatg cctctttttc actcggggag catgccagcg aataatcgcg
     2641 ccccagtatt gaaacatcga tgcgttccat tggaccttac tccgcagagg cgccgggcag
     2701 gcgcattaaa accatgtcaa ttcgatcttt tgccgattcg gccgcagtgc gcaagcgcgc
     2761 cgcttccgcc aacgcgaact gcaaattttg cctggctgtg gcacattcct cttgggcaga
     2821 caggccctga gcacgcaccg cttccagttc ggattcgtgc tgttcgatgc gccgtgccat
     2881 cgattggtac tcgctttccc ggcggtcgag ttgatcgcgc aaagcgccgc gctcttgctc
     2941 tgcgcttttc aggcgcgccc gcaacaccgt gctctcggcc tgaagctcgc gcgtaagctg
     3001 caccatttgc ccgattcggg cggcgaggga atcaagatct tgcaacatgg gcgctatggt
     3061 aaaccatcct gccccgggct ggatagagga aacgcgtatc gaaataaaag aatcattcaa
     3121 tgcgccgcaa tgcttcttcg cttcaataag ggaaattccg gtttccaatg cttcgcgaat
     3181 caattaccat catgcatctt ttcagtccaa tttcagtgag tttctagtta gcctgcgctc
     3241 gtagctgccc tgcgcatcgg ggcgccgagt gcttgaatga gcttgccacc cgtttacaaa
     3301 aagcgcccaa cctgtatcta gaggaggacc caatcaacat gcggcttaaa aataaacagg
     3361 acttctggtc cggaattatg ttcatggcga tcggagccgg attcgccatt ggcgcttcca
     3421 cctattccat gggcacatcg gcaagaatgg gccccgggta cttcccattc tggctaggca
     3481 tatgcctggc ggcgctcggt gcgctcgttt cacttaccgc attgtcaaaa tcggccgagc
     3541 acacgccggt cgaaaaattc gactggaaaa ttttgggtat cgtcatcgga tccgtcgtcg
     3601 gctgcggggt cgtcctgaat tttctgggtg tgtatgtggc gatttttctg ctggttttct
     3661 tcagcagcat ggcgagccac atattcagtt ggaagatagc cgtcatcaac ggaatttgcc
     3721 tgatggtttt cgtctggcta gcattcatca agggtctagg attgatcttc cccttgtggc
     3781 ccagctttct gagcatttag gagataaccc gaaatggaag tattcgatca tctcatgctg
     3841 ggctttcagg tagcgctgtc gcccgaaaat gtgttgtatt gcctgctggg atgcctgttg
     3901 ggccccctga tcggcgtgct gcccggcatc gggccggtgc ccaccatcgc catgttgctg
     3961 ccaatcacgt atgttctgcc ccccgtagcc ggcctgatca tgcttgccgg catttattat
     4021 ggagcccaat acggaggctc gaccacggcc attctggtgg ccatcccggg agaaaccgcc
     4081 gccgtggtga ccgtgctcga cgggcaccag atggcgcgca acgggagggc gggcgcggcg
     4141 ctggcaattg ctgctttagg gtcattcttc gcaggctgcg tcgccacggt catcatcgcc
     4201 ggattcgcgc cgccgctgtc ggaaattgct ttcaaattcg gcccggcgga atacttttca
     4261 ctgatggtgc tgggcttgat cggggccgtc gtgctggcct cagggtcact ggccaaagca
     4321 atctgcatga ttttgcttgg tttgttgctg ggaattgtgg gcaccgatgt caactcgggg
     4381 gtggcgcggt tcgatttcgg catccccgaa ttgcaggacg ggattgattt tgccgcagtc
     4441 gccatggggg tcttcggctt cgccgaaatc atgagcaacc tggaaatgaa agatcagcgc
     4501 gtcgatctca ccagcaaaat aggcagcctg tatcccaaca agcaggaatt caaagaatca
     4561 tggccggctg tcgttcgtgg aacggcgctg ggctcagcgc tggggattct gccaggcggc
     4621 ggcgcagtcc tttcatcatt tgcctcgtac acgctcgaga aaaaaatatc gcgctccccc
     4681 gaacgtttcg gcaaagggca tccggcaggg ttggccggac ccgagtccgc
//
LOCUS       DATCXU010000109         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_594615,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000109 DATCXU010000000
VERSION     DATCXU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594615"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VIR04_02400"
     CDS             complement(<1..842)
                     /locus_tag="VIR04_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA CCA-pyrophosphorylase"
                     /protein_id="HEY9459632.1"
                     /translation="MADADKSALTDPATDGLQVYIVGGAVRDELLGLPAGDRDWVVVG
                     TTPEAMAERGFVPVGGDFPVFLHPRTKEEYALARTERKSGRGYKGFTFHTGADVTLED
                     DLKRRDLTINAIARAPDGALIDPLGGVRDIRNKVLRHVGRAFVEDPVRLLRLARFAAR
                     FHDFTIAPRTLELGRQLVDDGEVDALVPERVWRELAKGLDTERPVRLFDVLRATGALA
                     RVLPGLVFNDAIGAPLACASRAGLSLAARFALLCRLSDEPGLIGKHVRAPADCQDYAR
                     LLPAV"
     gene            complement(842..>2811)
                     /locus_tag="VIR04_02405"
     CDS             complement(842..>2811)
                     /locus_tag="VIR04_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY9459633.1"
                     /translation="AINMDVQTAPEQPVQISAPPLQAIKPASTVPSPVARQALIDARE
                     AARRKQWTTLAGLVPLAQEDPQLGMYAQYWSLSEQLQDASSPIPEAELQQFMAANQDA
                     YLTERLKADWILAAARRNDYATVLRLGPIANSNAHVDCARLLAQHMTGQLVSSSQAMA
                     AFAPGRSCWAMLDQLVSDKVVGRAQLQPVLRATLETNKAADARRLAGYLFTSSEMSAY
                     RALISNPRKWLSTQNTPTTPAQIELVTLALSRLARDKDRDRSADYIKNNWARAIPKSN
                     IEWVWGQFGLVAALNVDADASQWYRRSGSASMTDYNHAWQVRSELRRAVIDWAWVGKA
                     IKKMSTRQQAEPVWVYWYGRALKGQGNSADADKYFASIENKMDFYGQLANEELGRSLS
                     IPPMPPAVTPAELAQARSNAGLQRAIALFDLDWRPEAVPEWNFALRGMTDRQLLAAAE
                     LARQQQIFDRVVNTSLQTKELVDFAQRFIAPFEGRVAAKAQLINLDPAWVYGLIRQES
                     RFITNAKSNVGASGLMQLMPATAKWVARKMGMNDFTPASVNDFDTNTVLGTNYLSMVL
                     NDLDGSQVLATAGYNAGPRRPMQWRSKLAGPVEGAIFAETIPFTETRLYVKHVMSNAT
                     YYAMMFSGKSDSLKERLGTISPEPDTRINLP"
BASE COUNT          598 a          914 c          760 g          539 t
ORIGIN      
        1 accgccggta aaaggcgcgc gtaatcctgg caatctgccg gcgcgcgaac atgtttgcct
       61 atcaaacccg gctcatcgga caaccggcat aacagcgcaa accgcgccgc cagactcaag
      121 ccagcccggc tggcgcacgc caatggagcg ccaatcgcat cgttaaacac caaaccgggc
      181 agcacgcgcg ccagcgcacc cgtcgcccgc aaaacatcaa acagccgcac cggccgttca
      241 gtgtccaagc ctttcgccag ctcgcgccaa acgcgttccg gcaccaaggc atcgacctcg
      301 ccatcgtcca ccaactgacg ccccagctcc agtgttcgcg gagcgatcgt aaaatcgtga
      361 aagcgcgccg caaagcgcgc cagacgcaac aggcgcacag ggtcttcaac aaacgcccgg
      421 cccacgtgcc gcaacacctt gttccggata tcgcgcaccc cgccaagcgg atctattaat
      481 gcgccatctg gcgcccgcgc aatggcgttg atcgtcagat cgcgcctttt cagatcgtct
      541 tccagcgtga catcggcgcc cgtatgaaac gtaaaacctt tgtagccgcg ccccgacttg
      601 cgctccgtgc gggccagcgc atattcctct ttggttcgcg gatgcaaaaa aacggggaaa
      661 tcgccaccga caggcacaaa cccgcgctcc gccatggctt cgggcgtcgt gcccaccact
      721 acccaatcgc ggtcgcccgc gggcaagccc agcaattcgt cccgaaccgc tccgccgaca
      781 atgtagacct gaaggccatc cgtggccggg tcggtcaagg ctgatttatc cgcgtcagcc
      841 atcagggcaa attgatgcgc gtgtcgggtt ccggcgatat cgtccccagg cgctccttca
      901 aagaatccga tttgcccgaa aacatcatgg cgtaataggt cgcgttggac atcacatgtt
      961 ttacgtacaa acgcgtttcg gtgaaaggta tggtttccgc aaaaattgcg ccttccacgg
     1021 gtcccgccag tttcgagcgc cattgcatgg gacgcctggg cccggcgtta taacccgccg
     1081 tcgccaacac ctgagaacca tccaggtcat ttaacaccat gctcagataa ttcgtgccca
     1141 aaacggtgtt cgtgtcgaaa tcattcaccg atgcgggcgt aaagtcgttc atgcccattt
     1201 tgcgcgccac ccatttggcc gttgccggca taagctgcat caggcccgac gcgccaacat
     1261 tcgatttcgc attggtgata aagcgggact cctgacgaat cagcccatat acccacgcag
     1321 ggtccagatt aatcagttgc gcctttgccg ccaccctgcc ttcaaacgga gcaataaagc
     1381 gctgcgcgaa atcgacaagt tctttggttt gcaacgacgt attgaccact cgatcaaata
     1441 tttgctgctg gcgcgccagt tcggccgccg ccagaagctg gcgatcggtc atgccgcgca
     1501 aggcgaagtt ccattcgggc actgcttcag gacgccagtc cagatcgaac agggcaatgg
     1561 cccgctgcaa gccggcgttt gaacgggctt gcgccaattc agccggcgtt accgccggcg
     1621 gcattggagg aatcgaaagg gaccgaccca gttcttcatt ggccaactga ccataaaaat
     1681 ccatcttgtt ttcaatcgag gcgaaatact tatccgcgtc cgcggagttc ccctgcccct
     1741 tcagcgcacg gccgtaccag tacacccaaa ccggttcggc ttgttgccgg gtcgacattt
     1801 tttttatggc tttgcccacc cacgcccaat caataaccgc acgccgcaat tcggatcgaa
     1861 cttgccacgc atggttgtaa tccgtcatgg aggccgagcc cgagcgcctg taccattgcg
     1921 aggcatccgc gtcgacattc aaggcggcca ccaggccaaa ttggccccag acccattcaa
     1981 tgttggactt gggaatggcg cgcgcccagt tgttctttat gtaatccgcg cttcgatcgc
     2041 gatctttatc gcgcgccaat cgcgacaagg ccagggttac aagctcgatt tgcgccggcg
     2101 tcgtgggcgt attctgagtg gacagccatt tccttggatt gctgatcaaa gcccggtacg
     2161 ccgacatctc ggaagacgta aacaggtagc ccgccagccg gcgcgcatcg gccgccttgt
     2221 ttgtttcaag cgttgcacgc agcacaggct ggagctgagc ccggccaacc accttatccg
     2281 atacgagctg gtccagcatc gcccagcaac tgcgtccggg cgcgaacgcc gccatcgcct
     2341 gcgaagacga aaccagttgc cccgtcatgt gttgcgccag caggcgggcg caatcgacat
     2401 gggcattgct attcgcaatc ggccccagac gcagaaccgt ggcatagtcg ttgcggcgcg
     2461 cggcggcaag aatccagtcg gctttcaatc gctcggtcaa ataagcgtcc tgattggccg
     2521 ccatgaactg ctgcagctcc gcctcgggaa tcgggctgga agcgtcttgc aattgctcgc
     2581 ttaacgacca gtactgagca tacatgccca attgcgggtc ttcctgcgcc aacggaacca
     2641 ggcccgccag ggtcgtccac tgctttctgc gcgcagcctc gcgggcatcg atcagcgcct
     2701 gccgggcgac cggagacgga accgtcgagg ccggcttgat agcctgaagc ggaggagcgc
     2761 taatctgaac gggctgttcg ggcgcagttt gaacatccat atttatggca g
//
LOCUS       DATCXU010000110         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_732011,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000110 DATCXU010000000
VERSION     DATCXU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732011"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VIR04_02410"
     CDS             <1..1135
                     /locus_tag="VIR04_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEY9459634.1"
                     /translation="GADEQKGYELAIEQLNAGAEQIKKISPLTKKGILGKTVKYGVVD
                     AETKPNSAVQGATRFIHENKAMIISGSVSSAVAIALQKVCDREKTIYLAAISGSNETT
                     GKDCQRYGFRLCYFAYSAAKAIAPVLAKSLGKNRKAVYLVPDYTYGHTTYDSTKEFTE
                     KEGWKTMGQQVHPLGAKDYSSYLINIANSGADTLVVIDYGADAANSIKQAKQFGILDK
                     MALVVPYMAPFLGEEVGPDIIEGVYGATGFWWTLQDKYPIAKDFVTAFEKKFGGKPHD
                     SAYIAYLNTALWADAIERAGTFYPPDVIKSYEKGVKRQGPTGEVWFRAEDHQGVINFP
                     IVRGKKKSEMKDKNDIFEIVAVVNGEEANPPVGHYGCKLGSYV"
     gene            1184..2059
                     /locus_tag="VIR04_02415"
     CDS             1184..2059
                     /locus_tag="VIR04_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459635.1"
                     /translation="MPSMSLFLSQLFNGLATGLLLALISTGLTIIYGTLGVVNFAHGA
                     LFVVGAYAGYTTYGLTDSFVLAIVAGAAAALVIGLIIERGVVRLYYSRPPEDQILVTF
                     GLGIILVELVRGIYGGVSLSVPAPSWGQGVVHIGTLIYPLYRFQTVFIAAGTLVGLYF
                     VLYRTSLGLIVRAGIEDSLMVNILGINVKRVFLLVFGIGAMAAGYAGVIDSPIVTLAP
                     DMGFRILVDSFVVVVIGGVGSFPGAIIGGIIAGEILSLTSMFDPAYSDVMLFVVMALV
                     LIFRPQGIMGQEGRE"
     gene            2056..>3092
                     /locus_tag="VIR04_02420"
     CDS             2056..>3092
                     /locus_tag="VIR04_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459636.1"
                     /translation="MNPNNRPGALMRRQIPELIVGLCLIVAPFTLPIIGISPDLMTRI
                     LIWGLFGLGFDLLFGYTGLLSFGQAAFFGTGGFVSCYLLTVGLVPNVLLALLIGTVVA
                     SFAGLIIGYLTLRRTGIYFAMSTLAFGEMFYFLENSSFKEYTGGENGIAGVPPPEIGL
                     GFTTLHISSGWPMYWLVALFFYVGYLVARRIVRSPFGAVLKAIRGNPKRAIALGHSIK
                     EYKLTVFVIASAYGGLAGGLLGIFQAYMPPDAFSLDTSSQLVIQTVMGGAGTLLGPLL
                     GATIWLYLYEGLQQIAGVGAWWKLILGIVFVVIVTLFHRGVVGAFVDGIRLLRKPSDT
                     ARRRARTDDRPL"
BASE COUNT          607 a          796 c          889 g          800 t
ORIGIN      
        1 aggcgcggac gagcaaaaag gctatgaact tgctatcgag caattaaatg ccggcgccga
       61 gcaaatcaag aaaatctcgc cgctgaccaa aaaggggatt ttgggcaaaa cggttaaata
      121 cggcgtcgtc gatgccgaaa caaagcccaa ctcggctgtg cagggagcca cgcgcttcat
      181 tcacgaaaat aaagcgatga taatctcggg cagcgtcagc agcgccgtgg ccattgcctt
      241 gcaaaaggtg tgtgatcgcg aaaaaacaat ttatctggcg gccattagcg gttcaaacga
      301 aaccacgggc aaagactgcc agcgctatgg tttccgcctg tgctacttcg cgtattcggc
      361 cgccaaggcc atcgcgccgg ttctggccaa atctctggga aaaaatcgca aggctgtata
      421 tttggtcccc gattacacct acggacacac cacgtacgac tcgaccaaag agttcacgga
      481 aaaggaaggc tggaaaacta tggggcagca ggttcacccg ctgggcgcca aagactacag
      541 ttcttatctg atcaatatcg ccaatagcgg cgccgatacg ctggtcgtca tcgattacgg
      601 cgctgacgcc gccaattcaa tcaaacaggc caagcagttc ggcattctcg acaaaatggc
      661 tctggttgtg ccgtatatgg ctccattcct gggcgaagaa gtcggccccg acattattga
      721 aggcgtttat ggcgcaaccg gcttctggtg gacgttgcaa gacaaatacc ccatcgccaa
      781 agacttcgtt actgccttcg agaaaaaatt cggcggcaag ccgcacgatt ccgcttacat
      841 tgcctacctg aacacggcat tgtgggccga tgccatcgaa cgcgcaggaa ccttctatcc
      901 gcccgatgtc atcaaatcct acgaaaaggg cgtaaagcgc caaggtccga ccggcgaagt
      961 ctggttccgc gccgaagatc accagggcgt catcaacttc ccgatcgtgc gtggcaagaa
     1021 gaagtccgaa atgaaagaca agaacgacat tttcgagatt gtcgctgtag ttaacggcga
     1081 agaagccaac ccaccggtgg gtcactacgg ctgcaagctg ggttcatacg tttaacgtaa
     1141 tagcttgcgg ttgaacgtgg tttatcaaaa agagggggat tgaatgccaa gtatgtcgtt
     1201 gtttttatca cagctattta acgggctcgc cacgggcctg ttgctggctt taatcagtac
     1261 cggcttgaca attatctacg gcacgcttgg cgttgttaat tttgctcacg gggctctgtt
     1321 tgtggtcggg gcctacgcgg gttacacgac ttacggcctc acggattcgt tcgtgctggc
     1381 tatcgtggct ggcgctgcag cggcgcttgt catcggtctg ataattgaac gcggcgtggt
     1441 gcgtttgtac tattcgcgcc cgccggaaga tcaaattctg gttacgttcg gcctgggtat
     1501 tattctggtc gaacttgtcc gcggcatata cggtggcgtc agcctgtccg tgcctgctcc
     1561 gtcgtggggc cagggcgttg tgcacattgg cacgctcatt tatccgcttt atcggtttca
     1621 gaccgtattc attgcggccg gcacactggt ggggctttat ttcgtgctgt atcgcaccag
     1681 cctgggcctg attgtacggg cggggatcga agactcgctc atggttaata ttttaggtat
     1741 taacgtcaag cgcgttttct tattggtgtt tggcatcggg gcgatggcgg cgggctatgc
     1801 cggcgtcatt gattcgccta ttgtcacgct ggcgcccgat atgggtttcc gaatacttgt
     1861 tgattcgttc gttgtggtgg tcatcggtgg tgtggggtcc ttcccgggcg ccatcattgg
     1921 gggcattatt gcgggtgaaa ttcttagcct tacttccatg ttcgatcctg cttattccga
     1981 tgtaatgtta tttgttgtga tggctctcgt cttaatattc cggcctcagg gaattatggg
     2041 acaggagggt cgtgaatgaa tccgaataat cgtcctggcg cgctgatgcg ccggcagatt
     2101 cccgaactga ttgttggctt atgcctcatc gtcgcgccgt tcaccctgcc tattatcggt
     2161 attagccccg acctcatgac gcgtatcctg atctgggggc tgtttgggtt gggtttcgac
     2221 ctgttgtttg gttacacggg cctgttgtcc tttggtcagg ccgccttttt cggcacgggc
     2281 ggttttgtgt catgttattt gctgactgtg ggcctggtgc cgaatgtgct gctggcgctt
     2341 ctgattggca cggttgtcgc ttcgtttgcg gggctgatca tcggctacct gaccttgcgc
     2401 cgcaccggca tttacttcgc catgagcacc ctggctttcg gcgaaatgtt ttacttcctt
     2461 gaaaattcgt cattcaaaga gtacacgggc ggtgaaaacg gtatcgccgg cgtgccgcct
     2521 ccggaaatcg gactggggtt cacgaccttg cacatttcca gcggctggcc catgtactgg
     2581 ctggtggcac tgttctttta tgtcggctat ctggtcgcgc gccgtattgt ccgttcgcct
     2641 ttcggcgcgg tgctcaaggc tattcgcggc aatcccaagc gggccattgc gctggggcat
     2701 tcaatcaagg aatataaact gaccgtcttt gtcattgctt ccgcttatgg cggtctggcg
     2761 ggcggcttgc tgggtatctt ccaggcgtac atgccgcctg atgctttttc gctggatacc
     2821 tcttctcaac tggtgattca aacggttatg ggcggtgcag gaacgctctt ggggcctttg
     2881 ctgggcgcga caatctggct ctatctgtac gaagggttgc agcagattgc cggtgttggc
     2941 gcgtggtgga agctcattct gggtattgtg ttcgtcgtaa ttgtcacgct ttttcaccgc
     3001 ggagtcgtcg gcgcctttgt cgacggcata cggcttttgc gcaagccttc cgacacggct
     3061 cgcagacggg cgcggacaga cgacaggccg ct
//
LOCUS       DATCXU010000111         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_148322,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000111 DATCXU010000000
VERSION     DATCXU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148322"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VIR04_02425"
     CDS             complement(<1..287)
                     /locus_tag="VIR04_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY9459637.1"
                     /translation="MNQAKKKEPESPVGVIGLGSMGFGVASSLLKAGFPVVGCDVRAD
                     VLDRFSKLGGISVRTPAELAQKVGALIVLVVDAAQTDMVLFGENGAAAAMQA"
     gene            complement(364..1170)
                     /locus_tag="VIR04_02430"
     CDS             complement(364..1170)
                     /locus_tag="VIR04_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY9459638.1"
                     /translation="MSNASASGIRPLKNMANMPDRVTEALQGLIRSGEFPPKSRLPAE
                     TEMAARFGVSRTVIREAVSRLKSEGLVQSRQGSGVFVRESNLDAPFRINPIVADSVLA
                     ALQIVELRRGLESEIAALAAERRSKKQAAEIMEKLRAIDADVAAGNDGVNADMEFHRS
                     IARAAGNPHFLALWDFVSQFLKKTMTITRSLEARKEPLALQVLQEHELLVDAIVRKDI
                     EGARAAARNHMEMAAIRIKSANPTSLKTGNGARAEAPAKKSGRAKPKIKQ"
     gene            complement(1304..2179)
                     /locus_tag="VIR04_02435"
     CDS             complement(1304..2179)
                     /locus_tag="VIR04_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY9459639.1"
                     /translation="MSKQSTDEKQGMDFLQSLPRRWVTIYIPLAIFVFVLMFPFYWMV
                     ITSFKPDSEIALSTSNPFWIVAPTLDHFKNLIFHTEFNEWLLNTVIISVVATFISLFC
                     SVFAAYAIERLRFQGSKQVGFAIFFAYLVPPSILFIPLASIIFQLGLFDTRWALILTY
                     PTFLIPFSTWLLMGYFRSIPYELEECALIDGATRWQILVKIILPLSVPGLISAGIFAF
                     TLSWNEYIYALTFISSSEEKTVAVGVVTGLIEGDVYHWGELMAGALLGSVPVAIVYSF
                     FVEYYVSGMTGAVKE"
     gene            complement(2176..3075)
                     /locus_tag="VIR04_02440"
     CDS             complement(2176..3075)
                     /locus_tag="VIR04_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY9459640.1"
                     /translation="MLSRRLNNSNVLGVLFMLPAAVLLLFFLTYPLGLGVWLGSTDTK
                     LGTTGNWVGLDNYIYLFGDPIAMLALLNTIFYTLLASVLKFMLGLWLALLLNKHLPLK
                     TIFRSIVLLPWIVPTALSALAFWWIYDAQFSIISWTLIKLGLIDHYINFLGDPWNARF
                     STVAANVWRGIPFVAITLLAGLQTISPALYEAASIDGVTPWQQFRYVTLPLLTPIIAV
                     VMTFSVLFTFTDFQLIYVLTRGGPLNATHLMTTLSFQRAIPGGALGEGSALAIMMIPF
                     LLGAIMFSYFGLQRRAWQRGGDK"
     gene            complement(3173..4492)
                     /locus_tag="VIR04_02445"
     CDS             complement(3173..4492)
                     /locus_tag="VIR04_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9459641.1"
                     /translation="MTKQTRRDFLKSTATVAAASALGIGGNVRAATPTLTYKPEDGAK
                     LRVLRWRRFVQGEEDAWMANTKKFTEKTGVEVKIDNEGFEDVRPKAAVAARVGVGPDI
                     ILGWYDDPQQYPDKLVDVSDLANYLGEKNGGWYDVCKKYGMQGDRWIALPLGVIGNEL
                     VYRKSMINAAGFDTVPKDTAGFLKMCQALKTKGTPVGFALGHAVLDGNAWVHWVLWSH
                     GGMMVDKAGKVAINSPETWAALEYAKELYKTFLPGTLSWQDPSNNKAFFDGQISLTNN
                     GISVYYSAMNSKDAKQHAMTDDIHHALYPIGPVGKPTELPGITQMMLFKYCKYPNAAK
                     AFMQYMLEPEQYNPWLKASIGYVSQSLKGYEANPIWTSDPKITPYRDAAARMIDNGYS
                     GPLGASSAAVMADYIVLDMFAGAASGSKTPKAAAAEAAERAKRYYKA"
     gene            complement(4585..>4962)
                     /locus_tag="VIR04_02450"
     CDS             complement(4585..>4962)
                     /locus_tag="VIR04_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY9459642.1"
                     /translation="GSPSMNFLPAVLRRDATGTQVEFSDGTRMPAPRRLTSGTEGQRI
                     IYGVRPEHLIIDPSHQGIKTDVTVVEPTGANTEVYCRFSNTDLVSIFRERHDFKPGDT
                     LSLVPDQERTHLFDADTGVALAA"
BASE COUNT         1083 a         1356 c         1390 g         1133 t
ORIGIN      
        1 gcttgcatgg ctgccgccgc gccgttttca ccaaacagaa ccatgtccgt ctgggccgcg
       61 tcgaccacca gaacgatgag tgcgccgact ttttgcgcca attccgcggg cgtccgaacg
      121 gaaatgccgc ccagttttga aaatcggtcc agtacgtcgg cgcgaacatc gcatccaacc
      181 actggaaacc ctgctttgag caaagacgag gcaactccga atcccataga ccccaatccg
      241 ataacgccca ccggcgactc cggctctttc ttcttcgctt ggttcattgc tttatggctc
      301 ctgatgaaac actgaaaatt agggtcgccg gcactgccgt ggcgccggaa ttgccgaatg
      361 agtttattgt ttgatttttg gtttggctcg cccgcttttt tttgccggcg cttcggcccg
      421 cgcgccattg ccggttttga gcgaagtcgg attggcgctt ttgattctga tcgccgccat
      481 ttccatgtgg ttgcgtgcgg cggcgcgggc gccttcaatg tctttgcgca cgattgcgtc
      541 caccagtaac tcgtgttctt gcagcacctg caatgctaat ggctctttac gcgcttcaag
      601 agagcgagta atcgtcatgg tttttttcag gaattggctg acgaagtccc acagcgccaa
      661 aaaatgaggg ttgcctgccg cccgtgcgat actgcggtga aactccatat cggcgtttac
      721 cccgtcgttg ccggcggcca catccgcgtc aatggcccgc aatttttcca tgatttcggc
      781 cgcttgcttt ttgctgcgac gctcggcggc gagggcggca atttcggatt caagtccgcg
      841 ccgcaattct acaatttgca gcgcagccag aactgaatcg gccacgattg ggttaatccg
      901 aaacggcgcg tcaagattgc tctcgcgcac gaacacgccg ctgccctggc gcgattgcac
      961 caagccttcc gactttaaac gcgataccgc ctcacggatc acggttcggc ttacgccgaa
     1021 gcgggcggcc atttcggtct cggccgggag ccgcgatttg ggaggaaatt cgccgctacg
     1081 gatcaggcct tgcaacgcct cggttacccg atcgggcata ttggccatgt ttttcagcgg
     1141 cctgattcct gatgccgatg cattggacat ttatacgctc cctgcaacgt gaagcccatt
     1201 tgcttgatat attattatca tacaactatg tacccggcgg gtttgttcgg gaggggccgc
     1261 ggccgcaacc ccgtcgacat taacccgatg gaaccctgaa agtttattct ttcactgcgc
     1321 cggtcatgcc cgaaacgtag tattcgacga agaacgaata cacaatggcc actggaaccg
     1381 accctagcaa ggctcccgcc atgagttccc cccaatggta gacatcgcct tctatcagac
     1441 cggtgaccac ccctaccgcc acagttttct cttcagagga cgagataaaa gttagcgcgt
     1501 agatatattc gttccacgac agagtgaacg cgaaaatacc cgctgaaatc aggcccggca
     1561 ctgatagagg caagattatt ttcaccagga tttgccagcg ggtggcgccg tcaatgaggg
     1621 cgcactcttc aagttcatag ggaatggatc tgaagtaccc cattaacagc catgtgctga
     1681 acgggatcag gaaggtgggg taggtcagta tcaacgccca acgcgtatcg aacagtccca
     1741 gttgaaatat gattgacgcc agtggaatga atagaatcga cggaggcacc aggtaggcga
     1801 aaaagatggc aaatccgact tgcttggatc cctgaaagcg caaccgttcg atggcgtatg
     1861 ccgcgaagac gctgcaaaac agcgatatga acgtggccac gaccgagatg atcacggtat
     1921 tgagcagcca ttcgttgaat tctgtatgga aaatcaggtt cttgaaatgg tccagggtgg
     1981 gagcaacaat ccagaagggg ttgctggtgc ttagcgctat ttcagagtcg ggtttgaaag
     2041 aggtgatcac catccagtaa aacgggaaca tcaggacgaa aacgaaaatg gctagcggta
     2101 tgtaaatcgt tacccagcgc cggggcagcg attggagaaa atccattccc tgtttttcat
     2161 ctgtggattg cttgctcatt tgtcaccccc gcgttgccag gcccgacgct gcaagccgaa
     2221 ataggaaaac atgatggcgc ccaacaggaa cgggatcatc attatggcca gagccgaccc
     2281 ttcgccgagc gcgccgcccg gaatcgcgcg ctggaatgac agcgttgtca tgaggtgcgt
     2341 tgcgtttaat ggtccgccgc gggtcaacac gtaaatcaac tggaagtcgg tgaacgtgaa
     2401 cagtacggag aaggtcatga ccacggcaat gattggtgtg agcagcggca atgtgacata
     2461 gcggaactgc tgccagggcg ttacgccatc gattgatgcc gcttcgtaca aagcgggaga
     2521 aatagtttgc aatccggcca ataaagtgat ggccacgaac gggatgccgc gccagacgtt
     2581 ggcagcgacg gtcgagaacc gggcattcca tgggtcgccc aggaaattga tgtaatgatc
     2641 gataagccca agttttatca aggtccaact gataatggaa aattgagcgt cgtatatcca
     2701 ccagaatgcg agcgctgaaa gcgctgtcgg gacgatccac ggcaatagca cgatagagcg
     2761 gaaaatggtt tttaacggca aatgcttatt cagcaacagc gccagccaca aacccagcat
     2821 gaatttcagt acgctggcca ggagcgtgta gaaaatcgtg ttaagcagcg ccagcatcgc
     2881 tatggggtcg ccgaataaat aaatatagtt atccagcccg acccagttgc cggttgtgcc
     2941 caacttggtg tcggtcgaac ccagccagac gcccagcccc agcgggtagg tcaggaaaaa
     3001 caaaagcagc acagcggccg ggagcatgaa gagcaccccc agcacgttac tgttattcag
     3061 tctgcgcgat agcagcatta tcgtccccgt aaaaccagcc tggctgcgct tgaataaaga
     3121 ccttgcgcgg ccaggcggat cgtgcttagt gagtcacaaa aaagccgcgc ctttatgcct
     3181 tgtaatagcg tttggcgcgt tcggcggcct ccgccgctgc cgccttcggc gtttttgaac
     3241 cgctcgcggc ccccgcgaac atgtcaagca caatgtaatc ggccatcact gccgccgagg
     3301 acgcccccag aggcccggag tacccgttgt cgatcatgcg cgcagccgca tcgcggtaag
     3361 gcgtgatctt ggggtctgaa gtccagattg gattggcctc gtaacccttc agagactggc
     3421 tgacataacc aatcgaggcc tttagccagg ggttgtactg ctcgggctcc agcatgtatt
     3481 gcatgaacgc cttggcggca ttcgggtact tgcaatattt gaacagcatc atctgcgtga
     3541 tgccgggcag ttcagtgggc ttgccaacgg ggcctattgg atatagcgca tggtgaatgt
     3601 cgtcggtcat ggcgtgctgt ttggcatctt tcgaattcat tgccgagtaa tagaccgaga
     3661 tgccgttgtt tgtaaggctg atttgtccgt cgaagaatgc cttgttattg cttgggtcct
     3721 gccacgacag tgtgcccggc aagaatgttt tatagagctc cttggcatat tcgagcgcgg
     3781 cccaggtttc gggtgagtta atagccacct tcccggcttt gtcgaccatc atgccgccgt
     3841 gcgaccaaag cacccagtgc acccacgcat tgccgtccag aacggcgtgg cccagtgcga
     3901 aaccgacagg cgtgcctttg gttttaagcg cctgacacat tttgagaaag ccggccgtgt
     3961 ctttgggaac cgtgtcgaaa ccggcggcgt tgatcatgct tttgcggtaa accagttcgt
     4021 tgccgatcac gcccagaggc agagcaatcc agcggtcgcc ttgcattccg tattttttac
     4081 acacgtcgta ccagccgcca tttttttcac cgagatagtt ggccagatcg gatacgtcca
     4141 ccagcttgtc ggggtactgt tgcgggtcgt cgtaccagcc aaggataata tcggggccta
     4201 cgccgacccg tgcggcgacg gcggctttcg gtctgacatc ttcaaaccct tcgttgtcga
     4261 tctttacttc gaccccggtt ttctccgtga attttttggt gttggccatc caggcgtctt
     4321 cttcgccctg cacaaaacgc cgccatctta gtacgcgcag tttcgcgccg tcttcaggtt
     4381 tgtaagtcag ggttggcgtt gccgcccgaa cattgccgcc gatgcccagc gcggacgctg
     4441 ccgcaaccgt tgcggtggac ttcaggaaat cacgcctggt ctgtttcgtc atgtctctac
     4501 tcctttgatt aacgggatgt tgcccatgtt gcctgggttc tgtaataagc cgaactgcat
     4561 cgttgaacta cttcttgatt ttggttatgc cgccagcgcc acgccggtgt cggcgtcaaa
     4621 caggtgcgtg cgttcctggt ccggaaccag ggacagcgta tcgccgggct tgaagtcatg
     4681 gcgttcgcga aaaatcgaga ccaggtcggt gttgctgaag cggcaataaa cttccgtatt
     4741 ggcgccggtg ggttcgacca ctgtgacgtc ggtctttata ccctgatggc tgggatcaat
     4801 gatcagatgt tcgggtcgca ccccgtagat gatgcgctgg ccttcagttc cggaagtcag
     4861 tcggcgcggt gccggcatgc gggtgccgtc ggaaaattca acctgtgttc cggttgcgtc
     4921 gcggcgcaat acggcgggca ggaaattcat ggatggcgag cc
//
LOCUS       DATCXU010000112         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_171332,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000112 DATCXU010000000
VERSION     DATCXU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171332"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VIR04_02455"
     CDS             <1..723
                     /locus_tag="VIR04_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEY9459643.1"
                     /translation="GRAIAYQLAEAGFNLLINDVVADTTLNETLSGIRQRGARAEFVH
                     ADIGDVAGHHALSNAAYEHFGRLDALVNNAGIQVRQRGDLLDITPERFDEIMGVNLRG
                     NFFLTQHVSQRMLNDGQPTTGRSIVILSSSNAELVSVEKGEYCVSKSGLSMTAKLFAV
                     RLAQAGIAVFEIRPGLIRTDMTASVRDKYGALIDQGLSPVRRWGEPEDIGRTVTTLVS
                     GLIPFATGIAVDIDGGLLIPRL"
     gene            749..1546
                     /locus_tag="VIR04_02460"
     CDS             749..1546
                     /locus_tag="VIR04_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9459644.1"
                     /translation="MKMTKGALMGVLLGALMMVVGATSYAQSINDIVKRGKVLIGVNS
                     GAPPFSFVDADGKARGMDVDIANLFAHYLGVPAEVSSYTTAARIPALEAGKVDFMVAT
                     LGATPARAKVVMFTGSYNVFPLVIAARKEDAIKSMDDLKGKKVAIARGTPQEAALMRV
                     APKGIELSRFDDDLTATQALLTGQVGAVAIPETIYKEVLKSRPGADIAVKFSFHNDFQ
                     SIAVRKGSYELLQWLNTTLSYIKGNGELDAIAMKWVGHPMPKDMPVF"
     gene            1559..2272
                     /locus_tag="VIR04_02465"
     CDS             1559..2272
                     /locus_tag="VIR04_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9459645.1"
                     /translation="MAFELQFAPILDQSPALIQALWLTIKLSFSAIFLGGMVGLLAET
                     ARSMLSKRAGLVVNFYVEAIRNTPFLVQLYIIFFGLPVLGIRMPAVYAGLLAMVINLG
                     AYSAEIFRAGVESIHRSQIEAGLSLSLTRSQVFVHVILKPAIAKVWPSLTSQFTLTML
                     GSSVCSFISVQELSGEAATVQSLTFRSFEVYIVASALYLALALALKAMLTALGYFLFS
                     QRPRKSTANLGKAKVGARP"
BASE COUNT          506 a          687 c          633 g          511 t
ORIGIN      
        1 gggcgggcta tcgcctatca attggctgaa gcgggcttta acctgcttat caatgatgtt
       61 gtcgccgaca cgacactgaa cgaaacacta agcggcatcc gtcagcgcgg cgcgcgagcc
      121 gaattcgtgc atgccgacat cggcgacgtc gcaggccacc acgccttgtc caacgccgct
      181 tacgaacact tcggacgcct ggacgcgctg gtcaacaacg ccggtatcca ggtccggcaa
      241 cgcggcgatt tgcttgacat cacgcccgaa cgattcgacg aaatcatggg cgtgaatctg
      301 cgcggaaatt tcttcctgac gcaacacgtc tcgcaacgca tgctcaacga cgggcagccg
      361 accactggcc gcagcatcgt catcctgtca tcgtcaaacg cagagctcgt ctccgttgaa
      421 aaaggcgaat actgcgtatc caaaagcggc ctttccatga ccgccaaact atttgccgta
      481 cgcctggcgc aagccggaat tgccgtgttc gagatccgcc ccggcctgat ccgcaccgac
      541 atgaccgctt cagtccgcga taaatacggc gcactgatcg accagggcct ttcgcccgtt
      601 cggcgctggg gcgaacccga agacatcggc cgaaccgtca ccacgctcgt ctctggcctg
      661 attccgtttg caacaggaat cgcggtcgat atcgatggag gattgcttat cccccggctt
      721 tagcaacatc ccactaaagg agaagtgtat gaagatgaca aaaggcgccc tgatgggcgt
      781 actactgggc gcactcatga tggtggtggg tgcaaccagt tatgcacaat ctataaacga
      841 catcgtaaaa cgaggcaagg ttttaattgg cgtcaacagc ggagcgccac cgttcagctt
      901 tgttgacgcg gacggcaagg cgcgtggcat ggacgtggac atcgccaacc tctttgctca
      961 ttacctgggg gttccagccg aagtgtcaag ctacacaaca gcggcacgca tcccggccct
     1021 tgaagcgggc aaggtggatt tcatggtggc gacgctcggg gcgacacccg cccgggccaa
     1081 agttgtcatg ttcacgggtt cgtacaatgt tttcccgctg gtcattgcgg cccgcaaaga
     1141 agacgccatt aaatcgatgg atgacctgaa gggtaaaaaa gtggcaatcg ccagaggcac
     1201 cccccaggaa gctgcattga tgcgcgtggc ccccaaaggc attgagttat cgcgcttcga
     1261 tgacgatctg acggcaactc aggctttgct cacaggacag gttggggcgg tcgccatacc
     1321 cgagacaatc tacaaggagg ttttgaaatc gcggccgggc gcagacatcg ccgtcaagtt
     1381 ctcgttccac aacgacttcc agtccattgc tgtgcgcaag ggttcgtatg aactgcttca
     1441 gtggttaaac accacgctct catacatcaa aggcaatggg gaactcgatg ccatcgccat
     1501 gaagtgggtg ggacatccca tgcccaagga tatgcccgta ttctgattca caatcctcat
     1561 ggcatttgaa ctgcaattcg caccaatcct ggatcagtct cccgcgctga tccaggcctt
     1621 gtggctgacc atcaagctat ctttttcggc aatctttctg ggcggcatgg tcggcctcct
     1681 ggccgagacc gcgcgcagca tgctgtccaa aagagccggc ttggtcgtga atttctatgt
     1741 cgaggcgatc aggaacacac cgttcctcgt ccagttgtac atcatttttt ttggactccc
     1801 ggtgctggga atacggatgc cagccgtata cgccggttta ctggcgatgg tcatcaacct
     1861 tggagcctat agcgccgaga tattcagggc gggagtcgag tcaatccacc gctcacagat
     1921 tgaagcaggc ctgtctcttt cgctgacacg cagtcaagtc ttcgtgcacg ttatcctcaa
     1981 gcctgccatc gcgaaagtct ggccttcgct gaccagccaa ttcactctca ccatgctggg
     2041 gtccagcgta tgctcgttca tatcggttca ggagctcagc ggcgaggccg ccaccgtgca
     2101 gtcccttact tttcgcagtt ttgaagtgta catagtggcc tcggcacttt acctggcgct
     2161 tgctctcgcg ctaaaggcga tgctgacggc cttgggatat tttttgtttt ctcagcgccc
     2221 tcgcaaaagc accgccaatc ttgggaaggc gaaagtggga gcccggccat gatacgtccg
     2281 ttcggcctgc atgaactttt cttcctgttg caatccactg gctggaccgt actcctg
//
LOCUS       DATCXU010000113         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_763238,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000113 DATCXU010000000
VERSION     DATCXU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763238"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VIR04_02470"
     CDS             complement(<1..865)
                     /locus_tag="VIR04_02470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY9459646.1"
                     /translation="MELHTIKMPDIGEGIAEVELVQWHVQPGDRVLEDQLLAEVMTDK
                     ATVEIPSPVAGTVHSLGGQVGDILAVGSPLISLEVVDGQETVQAADDAQKAASGPASP
                     QRQEPAFAPEAGSPSLPLSQDFGQETAVGVGASPAVRARAKELGIELRLVPGNGPGGR
                     VTHADLDAYIVRRPREPLSMANTRQRASLDDETVLPVLGLRRKIAQKMQEAKRRIPHF
                     AYVEEVDVTELELLRATLNSRWSGSRGHLTLLPLLIRALVLALRAFPQVNARFDDDAG
                     VLTRFGAVHVGI"
     gene            complement(865..1908)
                     /locus_tag="VIR04_02475"
     CDS             complement(865..1908)
                     /locus_tag="VIR04_02475"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY9459647.1"
                     /translation="MTTEAELTSKTTSMTMIQALRSAMDVMLARDENVVVFGQDVGYF
                     GGVFRCTEGLQAKYGVTRVFDAPISEGGIVGAAVGMAAYGLRPVVEIQFADYCYPAVD
                     QLISEAARLRHRSAGEFSAPMTVRMPCGGGVFGGQTHSQSPEALFTHVSGLRTVIPSN
                     PYDAKGLLISSIENDDPVIFLEPKRLYNGPFDGDHDKPVVPWSKSPLSDVPDGYYAVP
                     LDSAAIFRPGSDLTVLAYGTMVFVAEAAVRQSGIDAEIIDLRSIWPLDFDTIMRSVKK
                     TGRCVVVHEATRTSGFGAELIALVQEHCFYQLEVPIQRVTGWDTPYPHAHEWAYFPGP
                     TRIAAAFRRAMEA"
     gene            complement(1905..3143)
                     /locus_tag="VIR04_02480"
     CDS             complement(1905..3143)
                     /locus_tag="VIR04_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEY9459648.1"
                     /translation="MNKPVSLRLHVPEPTARPGQETDFSYLHLAPAGQARRPPVQVEA
                     ADIHDLAYSLVRVLDDDGQALGPWAQPLDPALLRSGLKIMMKTRIFDARMLVAQRQKK
                     MSFYMQCLGEEAIGTAQALALNRDDMCFPTYRQQTLLMARDVSLQDLICQLLSNECDR
                     LRGRQLPVLYSVRDASFFSLSGNLATQFIQAVGWAMASAIKGDTRIASGWVGDGATAE
                     ADFQTALTFAHVYRAPVILNIVNNQWAISTFQTLAGGEDTTFAARGIGSGIASLRVDG
                     NDFLAVYAASQWAIERARGNLGPTLIEWVTYRGGAHSTSDDPSKYRPADDWSRFPLGD
                     PIERLKRHLMGLGVWSEQEHQDTQKELEAQIVAAQKEAERHGTLTAGRTSRPETMFDD
                     VYKGLPEHLRRQREQSRESS"
     gene            complement(3300..>4861)
                     /locus_tag="VIR04_02485"
     CDS             complement(3300..>4861)
                     /locus_tag="VIR04_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEY9459649.1"
                     /translation="AVVALWIAWFYCLLAGWGVPARRTFLMLTVVALAHIARLPMNAS
                     RLLSLVAFAVIVLDPWALMASGFWLSFGAVYVLMASPGWVGQRLGREAATTRQRVGRF
                     LVAASGLQLAITVGLMPLLALIFHQVSVASPLANAYAIPVISLVVTPLSLLFAAVSFV
                     PGLEWLATVFAWLGHAALHAIMIPTVWLSELGPASFDIPAAPFWLTVIALLGLALAVA
                     PYGFPMRNAAWLLMMPALFWRPERPLTGDWKLTALDVGQSSAVVIQTERHALLFDTGL
                     RSGPTSDQGARTIVPFMRASGINKLDVMVVSHADIDHAGGARSTLASTPVEQSFSSFD
                     LLSYLRRESGLLGESFDNVRPPLAMSACKAGLAWHVDGVAFEFLWPLYSSRLVKNNQR
                     NGHACVLRVSGRRHSVLLTSDIGVLQESSLLDQGLKAADVVLAAHHGSKNSSGAAFVA
                     RMRAAHVIAQAGAWNHYGHPSPLVQRRWEQAGAAFWRTDRDGAVQLRSNAGDLQAQSV
                     RASNRRYWQSR"
BASE COUNT         1106 a         1529 c         1336 g          890 t
ORIGIN      
        1 cgatcccgac atgaacggcg ccaaagcgag taagaacgcc cgcatcgtcg tcgaagcgcg
       61 cattaacctg agggaatgca cgtaaggcaa gcaccagggc gcggatcagc aacggcagca
      121 gagtcagatg tccgcgactg ccgctccatc gactgttaag cgttgcgcgc aacaactcaa
      181 gttccgtcac atcgacctct tcaacatagg cgaaatgcgg gatgcggcgc ttggcttcct
      241 gcatcttctg tgcgattttc cggcgcaagc ccagtacagg aaggaccgtc tcgtcatcaa
      301 ggctggcacg ctgtctggta ttcgccatcg acaaaggctc ccgcggcctg cgcacaatat
      361 acgcatccag atccgcatga gtgacccggc ccccgggacc attgcctgga accagccgca
      421 gttcgattcc aagctctttg gcgcgcgcgc gcaccgcggg tgaagcgccc actccaacgg
      481 ctgtttcctg gccaaaatcc tgcgaaagag gcagtgacgg ggaacccgct tcgggagcaa
      541 acgcaggctc ctgcctctgg ggcgacgccg gaccggacgc cgccttttgg gcgtcgtctg
      601 ctgcctgcac ggtctcttgg ccgtccacga cttcaagact aatcaacggg gaaccgaccg
      661 cgagaatgtc ccccacctgg ccgcccagcg agtgcaccgt cccggccacc ggcgagggga
      721 tttcaaccgt tgctttgtcg gtcatgactt cagccagcaa ttggtcctcg agaactctgt
      781 cgccgggttg cacatgccat tgcacgagct cgacttcggc gatgccctct ccgatatccg
      841 gcattttgat tgtatgaagt tccatcacgc ctccattgcg cgccggaacg cggcggcgat
      901 acgcgtgggt ccaggaaaat aagcccactc atgcgcgtgc gggtagggag tatcccaacc
      961 tgtcactcgt tgaatcggga cctcgagctg atagaaacaa tgctcctgca ccagagcaat
     1021 caattcggcg ccaaaaccgc tggtgcgcgt cgcttcgtgc accaccacgc aacgccccgt
     1081 ctttttgacc gacctcatga tcgtatcgaa atccagcggc cagatactgc gcaaatcaat
     1141 aatctcggca tcaatgcccg actgccgcac ggcggcttcg gccacgaaca ccatggtgcc
     1201 gtaggccagc accgtcaggt cggagcctgg acgaaaaatt gcggccgaat ccagcgggac
     1261 ggcgtaatag ccgtcaggca catcactcaa ggggcttttt gaccacggca caacaggctt
     1321 gtcgtgatcg ccgtcaaagg gcccgttata caagcgtttg ggctccagaa aaatcacggg
     1381 gtcgtcgttt tcaattgaag agatcaacaa gcctttcgca tcgtagggat tggaaggtat
     1441 tacggtgcgc aagccgctga cgtgcgtgaa cagcgcctct gggctttgac tatgggtttg
     1501 gccgccgaag acgccccctc cacagggcat gcgcaccgtc atcggcgcgc taaattcgcc
     1561 tgcggaacga tgacgcaagc gcgccgcctc ggagatgagc tgatccacgg ctggataaca
     1621 gtagtcggca aactggattt caaccaccgg acgcaagccg taggccgcca tgcccacggc
     1681 ggcgccgacg atgccgcctt cggaaatcgg ggcatcgaac acgcgcgtga cgccatactt
     1741 ggcctgcaat ccttcggtgc agcgaaacac gccgccaaag tatccgacat cctgtccgaa
     1801 caccaccaca ttttcatcac gcgccaacat gacgtccatg gccgagcgca gcgcctggat
     1861 catggtcatt gaagtcgttt ttgatgtcag ctcagcttcc gttgtcatga tgattccctt
     1921 gactgctcgc gctggcgtcg caaatgctcg ggcaatcctt tatacacatc gtcgaacatc
     1981 gtctcgggcc tggatgtccg gccggccgtc aaagttccgt ggcgttcagc ttctttttgc
     2041 gcggcaacaa tctgcgcctc cagctctttc tgtgtgtcct ggtgctcttg ctcggaccag
     2101 accccgaggc ccatcaaatg ccgcttcagc cgctcgatcg gatccccaag cggaaatcgg
     2161 gaccaatcat cggctggcct gtatttggaa gggtcgtctg aagtcgaatg cgcaccgcca
     2221 cggtaggtaa cccactcgat caatgtcggt cccaggttgc cgcgggcgcg ctcaatggcc
     2281 cattgcgacg ccgcgtaaac cgcaagaaag tcattgccat ccacccgcaa ggacgcaatg
     2341 ccgctaccga ttccccgcgc cgcgaaagtc gtgtcttcac ctccggccag agtctgaaac
     2401 gtcgatatgg cccactgatt attgacaata ttaagaatga ccggcgcgcg atagacatgg
     2461 gcaaaggtca aagccgtttg gaaatcggcc tcggccgtgg cgccgtcgcc gacccagccc
     2521 gaagcgattc ttgtgtcgcc tttgatggcc gaggccattg cccagcccac cgcctgaata
     2581 aactgagtgg ccagatttcc cgatagcgag aaaaaactgg cgtcgcgcac agaatacaaa
     2641 acaggcaact gccgcccccg cagacgatcg cactcgttgg acaaaagttg gcagatcaga
     2701 tcctgcagcg acacgtcgcg cgccatcagc agagtttgct gacgatacgt gggaaagcac
     2761 atatcgtcac gattcaacgc cagagcctgg gccgttccaa tggcttcctc acccaggcat
     2821 tgcatataaa acgacatttt tttttgccgt tgagcaacca gcatgcgcgc atcgaaaata
     2881 cgcgtcttca tcatgatttt tagcccggaa cgcagtaacg ccggatcgag cggttgcgcc
     2941 caggggccaa gcgcctggcc atcgtcatcg agcacacgca ccagagaata ggccaaatca
     3001 tgaatatcgg ccgcctcgac ctgtaccgga ggacggcgcg cctgaccagc aggcgccaga
     3061 tgcaaatacg aaaaatcggt ctcctggccc gggcgtgcgg taggttcggg cacatgcaat
     3121 cgcaatgaaa caggtttgtt catttctgct ccttcacctt cgaaagcctc ttgtggaatc
     3181 ttcgccagtg gcgctatctt ttgaatagcg catccatcat tatccttcca ccgtgaatcc
     3241 gcaacattac ccatattgac aggtcaagta ttaattcttg tgtgggtcat gcgatgaagt
     3301 taccggcttt gccagtaccg gcggtttgac gcccggacac tttgcgcctg caaatcacct
     3361 gcattcgagc gtaactgaac cgctccatcc cgatcggtgc gccagaaagc ggcgccggct
     3421 tgttcccaac gtcgttgcac caacgggctg gggtgtccgt agtgattcca tgcgccggct
     3481 tgcgcaataa cgtgcgccgc ccgcatgcgc gcgacgaagg ccgcaccgga agaatttttc
     3541 gacccatggt gcgccgccag cacgacatca gccgccttca gaccttgatc gagcaacgag
     3601 gactcttgca atacgccgat atcgctggtc agcaaaacag aatgcctgcg gccgcttacg
     3661 cgcagcacac atgcatggcc attgcgttga ttgtttttaa cgagacggct cgaatacaga
     3721 ggccataaaa actcgaacgc cactccgtcg acatgccagg cgaggcctgc tttacacgcc
     3781 gacatggcta gcggcgggcg cacattgtcg aacgattcgc ccagcagacc ggattcccgg
     3841 cgcagatagg agagcaaatc gaacgaactg aaggactgct cgaccggcgt tgatgccaac
     3901 gtgctgcgcg ccccgccggc gtgatcaata tcggcgtgcg ataccaccat tacgtcgagc
     3961 ttgtttatgc cgctggcgcg cataaaaggc actatcgtgc gcgcaccctg atccgaggtc
     4021 ggcccggaac gcaaacccgt atcgaacagc aaggcgtggc gctccgtctg aatcacaacc
     4081 gcgctcgatt gaccaacatc cagcgccgtc aatttccaat cgcctgttaa cggacgttca
     4141 gggcgccaaa acagggcagg catcatcagc agccaggccg cattgcgcat tggaaagcca
     4201 taaggagcaa cagccagcgc cagacccagc agcgcgatga cagtcagcca aaacggcgcg
     4261 gcgggtatgt cgaagctggc cgggccaagc tccgacagcc agactgtcgg aatcatgatc
     4321 gcatgcaaag cggcatgccc cagccatgca aaaacggttg ccagccattc caggccggga
     4381 acaaaagaga ccgctgcgaa cagcaaggag agcggcgtca ccaccaggct gatgaccgga
     4441 atggcgtagg cgtttgccag aggcgacgcc actgagacct gatgaaaaat cagtgccagc
     4501 agcggcatta gtccaaccgt tatggccagt tgcaagccgc tggcagcgac cagaaaacgg
     4561 cccacgcgtt gccgggtggt ggcggcctcc ctaccgagcc gctggcccac ccagcccgga
     4621 ctggccatta atacataaac cgcgccaaac gacagccaga atccactggc cattagtgcc
     4681 catggatcca gcacgattac ggcgaatgcc accaggctaa gcaggcgtga tgcgttcatc
     4741 ggcaagcggg caatgtgcgc cagcgccacg acagtcaaca tcagaaaagt gcgccgcgcc
     4801 ggaacccccc agccggccag caaacaataa aaccacgcaa tccacaacgc cacaacggcg
     4861 g
//
LOCUS       DATCXU010000114         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_417085,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000114 DATCXU010000000
VERSION     DATCXU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417085"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VIR04_02490"
     CDS             <1..626
                     /locus_tag="VIR04_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY9459650.1"
                     /translation="YWVCPLVEESETLQLQTAVDTYTALVEALPDLRVGLVHGRLSST
                     EKAQVMRDFGAGGIDLLVATTVIEVGLDVPNASLMIIEHAERFGLAQLHQLRGRVGRG
                     DVKSVCVLMYQRPLSHIARQRLRAMYETTDGFEIARRDLEQRGPGEFLGMRQSGIELF
                     RFASLQTDASIVDLAQSAARELRQHFPGHVRAHLHRWMRGKQDLLRG"
     gene            649..1605
                     /locus_tag="VIR04_02495"
     CDS             649..1605
                     /locus_tag="VIR04_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9459651.1"
                     /translation="MTLTELKYIVAVARQRHFGRAAEACFVSQPTLSVAIRKLEEELG
                     VVLFERGGSEVGITPVGLKVVDQAQRVLEESANLREVARQGHDPLSGPLRVGVIHTIG
                     PYLLPRLVPTQIALTPQMPLLLQENFTVRLLELLRQGEIDCAIVALPIPESGLVVHPL
                     YDEPFLVAVPKHHPWSKLKEINAFDLKNETMLLLGVGHCFRDQVLEVCPELSRYSASS
                     EGIQRTFEGSSLETIRHMVASGIGVTVLPTSSLSMPGLENNLLAYIPFRKPIPERRVA
                     LVWRKSYPRTTAIEALINAVRECSLPGVNYLCNVPVPELLHA"
     gene            1709..2191
                     /locus_tag="VIR04_02500"
     CDS             1709..2191
                     /locus_tag="VIR04_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY9459652.1"
                     /translation="MSDKSVLIDIGISEKDRAAVSDQLSRTLADSYTLYLMTHNFHWN
                     VTGPLFNTLHLMFMTQYTEEWQALDSIAERIRALGHYAPGTYAEYARLSSIAEPTSVP
                     DAEEMIQMLVMGNQAVAKTARVAFKCAEAANDQPTIDLLTQRLDIHEKNAWMLRSLLQ
                     "
BASE COUNT          498 a          612 c          653 g          553 t
ORIGIN      
        1 cctattgggt ttgcccgctg gtcgaggaaa gcgaaacttt gcagttgcaa actgccgtcg
       61 atacttatac ggcgctcgtt gaggcgctgc ccgatctgcg cgtcgggctt gtacatggcc
      121 gcctgagttc aactgaaaag gcgcaggtca tgcgcgattt cggagccggc ggaatcgatt
      181 tgctggtggc caccaccgtt atcgaggttg ggctggatgt gcccaatgca tctttaatga
      241 ttattgagca tgctgaacgt ttcggtctgg ctcaattgca tcagttgcgc ggccgcgtcg
      301 gacgcggcga tgtgaagtcg gtatgcgtgc tcatgtacca gaggccgctg tcgcacattg
      361 cgcgacagcg attacgggcc atgtatgaaa ccacggacgg atttgaaata gcgcgccgcg
      421 atctggaaca gcgcggccct ggtgaatttt tgggcatgcg gcagtcaggg atagagctat
      481 ttcgttttgc cagcctgcaa acggatgcct cgattgtcga tctggcccaa tcggccgcgc
      541 gcgagttgcg gcagcatttt cccggccacg tacgcgctca tctgcatcgc tggatgcggg
      601 gcaagcaaga tttattgcgt ggttgatttt ctttttgcga gataaagcat gactttgacg
      661 gaattaaaat acattgttgc ggtggctcgc caacgccatt tcggtcgcgc cgctgaagcc
      721 tgctttgtga gccagcccac gttgtccgtg gcaattcgca agctcgaaga ggaactgggc
      781 gtagtcctgt ttgagcgcgg cggctccgag gtgggcatca cccctgtcgg attgaaagtg
      841 gtggaccagg cgcaacgtgt ccttgaagaa agcgccaatt tgcgggaagt cgcgcgtcag
      901 ggacacgatc cgctgtcggg accgttgcgc gttggcgtga tccacacgat tggaccctat
      961 ttattgccgc gtctggtgcc cacgcaaatc gcgctaactc cacaaatgcc gctactgctc
     1021 caggagaatt ttacggttcg tttgctcgaa ctgctgcgtc agggcgaaat cgattgcgcc
     1081 attgtggcgt tgcccatacc tgaatcaggc ctggtcgtgc atcctttgta cgacgaacct
     1141 tttttagtcg cggtgcccaa acatcatccg tggtcgaagc tgaaggaaat taacgctttc
     1201 gaccttaaaa acgaaacaat gttgttattg ggtgttggtc attgcttccg cgaccaggtc
     1261 ctcgaggtat gtcccgaatt gtcccggtat tccgcctcca gcgaaggtat tcagcgtacg
     1321 ttcgaaggct cgtcgctaga aacgattcga cacatggtcg cttcgggcat cggcgttacc
     1381 gttttgccca ccagctcctt gtccatgccg ggtctggaaa ataatttgct ggcctatatc
     1441 ccattcagga agcctatccc ggaacggcgt gttgcactgg tctggcgtaa aagctatccg
     1501 cgaacgacgg ctattgaggc gctgattaat gccgtgcgcg aatgcagctt gccgggcgtg
     1561 aactatttgt gcaatgtgcc ggtgccggag ctgcttcatg cctgatcagt tttcggcatg
     1621 ttatgctgaa taaacccccg atttcgttca ttatttttcc gaggttcata tggcaaagaa
     1681 agctggaatg cgcagcaaaa tcgcgaaaat gagtgacaaa tcagttctta tcgatatcgg
     1741 tatctccgag aaagatcgcg ccgccgtcag cgaccagctt tccaggacgc tggctgattc
     1801 ttacaccttg tatctgatga cgcacaattt ccactggaac gtcacggggc ctctgttcaa
     1861 tactctgcac ctaatgttta tgacacagta caccgaggaa tggcaggcgc tcgacagcat
     1921 tgccgagcgt atccgcgcac tggggcacta cgcccccggc acatacgccg aatatgccag
     1981 actgtcatcc attgccgagc ccacctctgt gcccgacgcg gaagaaatga ttcaaatgct
     2041 ggttatgggt aatcaggcag ttgccaagac ggcgcgcgtt gcgtttaaat gcgcagaggc
     2101 agccaacgat caacccacga ttgatttgtt gactcaacgg ctcgacatac acgagaaaaa
     2161 cgcatggatg ttgcgcagct tgcttcagta acatggacaa taaacaggca cgctcaacgt
     2221 gtctgaaaac ggagaaaccc atgaaaattc atttcacacc cttagtcgcc gccctgggcc
     2281 tggctgcggg aatggcgatg tcgccgctgg cgggag
//
LOCUS       DATCXU010000115         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_493408,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000115 DATCXU010000000
VERSION     DATCXU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493408"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VIR04_02505"
     CDS             complement(<1..479)
                     /locus_tag="VIR04_02505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY9459653.1"
                     /translation="MAERSGLKKGLTDYGDIDFSLFLRKAFIKGAGYTDDALTRPIVG
                     ITNTGSGFNPCHGNMPQLIEAVKRGVMLAGGIPIDFPTISIHESFSYPTSMYLRNLMS
                     MDTEELIHAQPMDSVVLIGGCDKTVPAQLMGASSAGIPAIQLVTGSMLTGSHRGTRVG
                     "
     gene            607..750
                     /locus_tag="VIR04_02510"
                     /pseudo
     CDS             607..750
                     /locus_tag="VIR04_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
     gene            complement(786..1664)
                     /locus_tag="VIR04_02515"
     CDS             complement(786..1664)
                     /locus_tag="VIR04_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9459654.1"
                     /translation="MFDDFRNVATVIDGIRINAVLGGSGPPLLLLHGHPQTYAIWHKV
                     APALAHHFTVVATDLRGYGDSGKPAGLPDHSNYSKRRMAADQVELMQALGFGSFLVLA
                     HDRGARVAARMALDFPNAVRKLVTLDIAPTLAMYEQTSFEFSRAYWHWFFLVRPAPFP
                     EALIRADPELYMKQTMGSRSAGLKPFAPEAYAEYLRCLRDPATAHGLCEDYRASMTID
                     LEHDRADRDAGHKIGCELLALWGADGVIQKCFDPLAEWRRVAAHVDGGSLPCGHYIPE
                     EAPEKLLARVLPFLRE"
     gene            complement(1719..2450)
                     /locus_tag="VIR04_02520"
     CDS             complement(1719..2450)
                     /locus_tag="VIR04_02520"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459655.1"
                     /translation="MAPVLTVSGLAYSYATADRPIFKDLGLSVHAGEFIAIVGGSGVG
                     KSTLLRCIGRLLRPTAGEVSLNVVHDPSRRQVAFVFQDGRLMPWRRLRRNVAYGLKGL
                     SLTHQEREARLDEVLSLALISDLAQRWPHQLSGGQIQRAGIARALAVHPDLLLMDEPF
                     SAVDAITRQRLQDELLNIWGKTGAAVLFVTHDIDEAVYLADRVVVLSGSPASIKADRQ
                     VNLPRPRNRNAADLRQVAYSIMQDI"
     gene            complement(2435..3217)
                     /locus_tag="VIR04_02525"
     CDS             complement(2435..3217)
                     /locus_tag="VIR04_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459656.1"
                     /translation="MSDGTHFLTATRIGRAVLGFAGVVAFVAIWKFAQASGWAQKGTI
                     PDPFLLPRALLDEWTAGRLLPAIRSSLIHYVWGLSLGTLCGLSIGIAAALSTWVDAFH
                     SWLARILRPIPPLAWIVFAIAWFKVSHAGAAFVIAIGVFWINYFATYSAVRNVEPKYY
                     ELARAFGQDGFVAEMLSVTMPAASPGIFAGMRTGIGSAWMTLIAAELLGVSGMGQEMD
                     AAAGVGSYETVVIYMLAISLVYTLSDTMFKWVEKRALQWRPS"
     gene            complement(3228..4232)
                     /locus_tag="VIR04_02530"
     CDS             complement(3228..4232)
                     /locus_tag="VIR04_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459657.1"
                     /translation="MYRTVQLFVVLFLSSLFAANSQAADKLEVGYMPIISIAQEFVVL
                     ERKTATDPGLENAKLIEFQNGPAIVQALLAGQLDVAYLGIGPAMVARTKGADIKVVAA
                     STIESDSLLALGDLAHYFQNGDASTAFARFASDKGRKAVITTFPRGSVPETVLQYWLR
                     KQLKVDPDSVKVIYQGAAQVQQSLLTGAVDGASIMEPTVSIVTSRLKDARVVASGSGL
                     FPDQPASVLVVREKLIREKPELVRTLVAAHVEATELLRNKPEEAAPAVRKYVGHGRLP
                     LETVVLALQNSRLGFVSDPKKIIKSTQMMHDFQVEIGTLKGAMDVETMFDAQFFDQLK
                     "
     gene            complement(4380..>5082)
                     /locus_tag="VIR04_02535"
     CDS             complement(4380..>5082)
                     /locus_tag="VIR04_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY9459658.1"
                     /translation="MGDCFAQSSAEPAKDAAPPNFGLSANVSLASQYRVRGLMQTNSE
                     PAIQGGLDLSPSSGFYAGNWNSSITWLSGSAPVEMDFYGGYTKKIMGDLSLDVGAIRY
                     YYPGNTLGSPNTTELYLGLGYGPVSFKYSRSLTDLFGVDESKGSQYYDFKGSIDTGVW
                     GLMADGHVGYQKVRGTADASYTDWLLGVSKSWSNGFSASLAYIDTNADHAFYTNRQGK
                     YMGRSTVLLTVTRKF"
BASE COUNT         1235 a         1513 c         1303 g         1031 t
ORIGIN      
        1 cctacccgcg ttccgcggtg ggagccggtc aacatggacc cggtcaccaa ctgaatcgcc
       61 ggtatccctg ccgaagaggc gcccataagc tgcgcgggaa ctgtcttgtc gcaccccccg
      121 atcaatacaa ccgaatccat gggctgggca tgaattagtt cttcggtatc catcgacatc
      181 aggttacgca agtacatgct tgtcggatac gaaaagcttt catgaatgga aatggtgggg
      241 aaatcaatcg ggattccccc ggccagcatg acaccccgtt taacggcctc gatcaattgc
      301 ggcatatttc cgtgacaggg attaaatccg ctgccggtgt tcgtaatgcc cacgatgggc
      361 cgcgtcagcg catcgtcggt ataacctgcg cccttgataa acgcctttct tagaaataat
      421 gaaaaatcaa tatccccgta gtcagtaagc cctttcttca aacctgatcg ttccgccatg
      481 tgccccgccc tgccgttgtt tgtatcaata aagttattaa taatccatag acgatcataa
      541 gcgccttgcg gcacttgacg caaggcgctt caaacaatgc agccgattta atccctatat
      601 gcgaaaatgg tcggccgtcc tcttgccggg ctcgtctgcc tgattctgca aataacactg
      661 atcggctgga ttcctgccac gatttgggcg gtatacgcct taagccagta caagaccgac
      721 aaaaaaatag aaaaggcgtt gggctcacgt cgagccgcgt cgatatcggt caacggatcg
      781 cgccgctatt cgcgcaggaa cggcaatacg cgagcaagca gcttctcggg cgcctcttca
      841 gggatatagt gaccgcatgg cagcgatccg ccgtcaacat gcgcggccac ccgccgccat
      901 tctgcgagcg ggtcaaagca cttctggatt acgccgtccg caccccacaa ggcaagtagt
      961 tcgcaaccga tcttgtggcc ggcgtcccgg tcggcccgat catgttcaag atcaatcgtc
     1021 atgctcgcac gatagtcttc gcacaggccg tgcgcggtgg caggatcgcg caagcaacgc
     1081 aaatattcag cgtaagcctc cggcgcaaac ggtttcaggc ccgcacttcg gctgcccatc
     1141 gtctgcttca tatacaactc tggatcggca cgaataagcg cttccgggaa aggcgcaggg
     1201 cgcaccagga agaaccaatg ccagtatgcg cgcgaaaatt cgaacgaagt ctgctcatac
     1261 atggccagcg tcggcgcaat atccagcgta acgagcttgc gtacggcatt cggaaaatcc
     1321 agcgccatac gcgcagcgac acgcgcccct ctgtcgtgag cgagaaccag aaaagagccg
     1381 aaacccagcg cttgcatcaa ctcgacctga tcggccgcca tccgccgctt ggaataatta
     1441 ctatggtccg gaagcccggc tggcttgccg ctgtcgccat agccgcgcag atccgttgcc
     1501 acgacggtaa agtggtgtgc cagcgccgga gcgactttgt gccaaatggc ataagtttgc
     1561 ggatgcccat gtaaaagaag aagtggcggc ccggatccgc ccagcactgc attaatccgt
     1621 ataccgtcta ttactgtggc cacatttcga aaatcgtcga acacctttgt ctcccagaag
     1681 ctttggacag tttagttcct gactggaagc gatgccggtt atatgtcctg cattatggaa
     1741 taagcgactt gccgcaggtc cgcggcattc cgattgcgcg ggcgcggcaa attaacctgc
     1801 cggtccgcct tgatggaagc gggggagcca gaaagcacca ctacccgatc ggccagatac
     1861 acggcctcgt ctatgtcgtg agtaacgaaa agcactgctg cgcccgtctt gccccagata
     1921 tttaacaatt catcctgcag cctttggcgg gtgattgcat ccacggcgct gaagggttca
     1981 tccataagca gcagatccgg gtgcacggcc agtgcgcgcg cgataccggc tcgttgaatt
     2041 tgtccgcccg agagctgatg cggccaacgc tgcgccagat cggaaatcaa ggccagcgac
     2101 agcacttcat caaggcgcgc ctcacgttcc tgatgggtga gcgacaaacc tttaagaccg
     2161 taggccacgt tgcgtctcaa tcgtcgccag ggcattaaac gaccatcctg aaacacaaaa
     2221 gcaacttgtc gacgggatgg atcatgaacg acattcaacg aaacttcgcc cgctgttggc
     2281 cgcaacaatc ggccgatgca acgcagcaac gtcgacttcc ccactcccga gccgcccaca
     2341 atggcaataa attcgcccgc atgcaccgac aaaccaaggt ctttgaagat cggtctgtcc
     2401 gccgtcgcat aagaatacgc aaggccgctg acggtcagga cgggcgccat tgcaacgccc
     2461 ttttctcaac ccatttaaac atagtgtcgc tcagtgtata gaccagcgat atcgccagca
     2521 tgtagatcac caccgtctca taagagccga ctcccgcagc ggcgtccatc tcttgaccca
     2581 tgcccgaaac gcccagcaac tcggcggcga tcaatgtcat ccaggccgaa ccgatcccgg
     2641 tgcgcatgcc ggcgaagatg cctggcgatg cagcgggcat cgtgacgctg agcatttcag
     2701 ctacaaagcc gtcttggcca aaggcgcgcg ccagctcgta atattttggt tcgacattgc
     2761 gaactgcgga ataagtcgca aaataattaa tccagaacac tccgatcgca atgacgaaag
     2821 ccgcgcccgc gtgactgacc ttgaaccatg cgatcgcaaa cacgatccac gcaagcggcg
     2881 gaatcggccg caagatacgc gccagccacg aatgaaacgc atcaacccag gtcgacaacg
     2941 cagccgctat gccgattgac agcccacata gcgtgcccaa cgataaaccc cagacataat
     3001 gaatcagact ggagcggatt gcaggcaata gccggcccgc cgtccattca tccaacaagg
     3061 cccgcggcag caggaatgga tccgggatag tgcctttttg ggcccagccc gaggcctgcg
     3121 cgaacttcca gatcgccaca aacgctacga cgccggcgaa tccaagcacc gcgcggccaa
     3181 tacgggtcgc ggtcaaaaaa tgcgtaccgt ccgacatggc aattgcgcta tttcagttga
     3241 tcaaaaaatt gggcgtcgaa catggtttca acgtccatag cgcctttgag agttccgatt
     3301 tccacctgaa aatcatgcat catctgcgtg ctcttaatga tcttttttgg atccgacaca
     3361 aagcccagac gcgaattctg aagcgccaga accactgttt caagcggaag ccggccgtga
     3421 cccacatact tccgcaccgc gggcgccgcc tcttcgggtt tattgcgcag caactcggtt
     3481 gcttcgacat gggcggcgac cagtgtgcgc accagctcag gcttctcgcg gatcagcttc
     3541 tcacgcacta caagcacgga agccggctga tcagggaata ggcccgagcc gctggcaacg
     3601 actcgcgcat ctttcaggcg gctggtgaca atactgacgg tcggttccat aatcgaggcg
     3661 ccatccaccg ccccggtcaa taatgattgc tgaacctgcg ccgccccctg gtaaatcacc
     3721 ttgacactgt ccgggtccac cttgagctgc ttgcgcaacc aatactgcag caccgtttcg
     3781 ggcaccgaac ctcgtggaaa agtggtaatg accgccttgc gccccttatc gctggcgaaa
     3841 cgcgcgaacg cggtgctggc gtcgccattt tgaaagtaat gagccagatc gcccagcgcc
     3901 agaaggctgt ccgattctat cgtagacgcc gccactactt tgatatcggc ccctttggtg
     3961 cgcgccacca tagccggtcc gatgcccaga taagcgacgt ccagttgacc cgcgagcaag
     4021 gcctggacaa tcgccggccc attttgaaat tcaatcagct tggcgttttc aagaccgggg
     4081 tcggtcgccg ttttacgttc gagcacgacg aactcttgag caatcgaaat aatcggcata
     4141 tagccgacct cgagtttatc ggctgcctgc gagtttgcgg cgaacagcga cgacaaaaaa
     4201 agaacaacaa aaagctgaac agttcgatac atgaaaacac tcgaatttgg gcaatccgcc
     4261 atcttacata agaaaaattc atgagattgc ggttcggtta tattatttgg acatataaat
     4321 attcttggac cggcatacgt cggagaccgg tccgcgctga atcaataatc ttcatccagc
     4381 taaaacttcc tggtgacagt taaaagtaca gtcgaacgac ccatgtattt tccctgccga
     4441 tttgtataga aggcatgatc agcattggta tctatatagg caagcgaagc agaaaatccg
     4501 ttgctccagc tcttgcttac cccaagaagc caatcggtat acgaggcgtc ggccgtgccc
     4561 ctgacctttt gatagccaac atgtccatcc gccatcagcc cccacacgcc cgtatcaatg
     4621 cttcccttga agtcgtaata ctgactgccc ttgctttcgt ccacaccgaa aaggtcggta
     4681 agcgatcggg aatatttaaa cgacacgggg ccataaccaa ggccgaggta tagttcagtc
     4741 gtgttgggag aaccgagagt attgccgggg taataatacc gaatagcgcc cacatccaga
     4801 gacaggtctc ccataatttt ttttgtatag ccgccataga aatccatctc gacgggtgcc
     4861 gaccccgaaa gccacgtaat gctcgagttc cagttgcctg cgtaaaagcc gctggaagga
     4921 gacagatcca gaccaccctg tatggcaggt tcgctgttcg tctgcataag gccccggacg
     4981 cgatactggc tggccagcga gacgttggcg cttagaccga aattcggcgg cgccgcatct
     5041 ttggctggct ccgcactgct ttgggcaaag caatcgccca tc
//
LOCUS       DATCXU010000116         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_732333,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000116 DATCXU010000000
VERSION     DATCXU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732333"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VIR04_02540"
     CDS             <1..1253
                     /locus_tag="VIR04_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY9459659.1"
                     /translation="MFITAGILAAMGLIPNMPHVAFLSLALILGGAGWLLQGRQRIER
                     AQLAQEPLKNQAAIEAAAAEASWDDVALVDPLGLEVGYRLISLVDHEQNGELLHRIRG
                     LRKKFAQDVGFLPPVVHIRDNLELKPNDYRILLFGVEVGHGVATPGQWLAIDPGGVTM
                     KLPGEATTDPAFGLPAVWINVSLREQAQIAGYTVVDAGTVVATHLNHLMHRYGANLLG
                     RQEVQQLLDHIGKEAPKLVEDLVPKTVTLTLLQKLLRGLLDEEVAIRDVRTIIETLTE
                     HAPRLAALNPNGSGPDPAELLALVRVALGRAIVQQWFPGDGELRVIGLDARLERVLTQ
                     ALTTSGVLEPGLAENVALDAQRAVQVQESNGDSPVLVVPAALRVSLSRFLRHHLPQLG
                     VLSNAEIPDERILRVTCVIGGDKA"
     gene            1250..3559
                     /gene="flhF"
                     /locus_tag="VIR04_02545"
     CDS             1250..3559
                     /gene="flhF"
                     /locus_tag="VIR04_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEY9459660.1"
                     /translation="MNLSRFFGSTNREVLRQVRMALGPDALIVSNRRVNGGVEIMAAD
                     ATSLPAGVQDPDSNKVREPDSNVIEAIGAMRGALETRIDEVLWGSQLRRAPEAVQVFQ
                     TMLGCGFSTALLRAMLKRLPAHLSPGAAFQWARNEIVTHLPVLESENDLWAPGLALAL
                     VGPTGIGKTTTIAKLAARCAKRFGARNVTLLTTDTYRIGAHEQLKIYGRMMQLAVHVV
                     QDADELRRVVQELGPDQIILIDNVGVSQRDHYISQQASLLAAAGRPVKRILVVNASSN
                     GDTLDEVARSYSNDGGSPLTGCIITKLDEATRLAAALDTAIRYQLPIHYVSSGQKVPE
                     DLWLLSAADLVDRALAGPTQTRPLYAPTQADFAAIMSLVKVPQPDSTVADDRRKQLLP
                     SLLCAVGGANFVLSEPELKDACLFFDDDPACSKALDLWRDYVAPVSPAPTDSPIENSI
                     ENMVRCARNYLAGIEHRHVLAIHDCVSLKLRSNAHGHLRATALLTEHARALTSPIQQL
                     ALADGWRSSCGLTSLEAPRPSDALYHQVQWLAQRCDRMSLVHVFGSATPALRRRLSLE
                     GTAWLTQCPGATKVQFDSCATTLSALAKTLVFRPMEQPGQSVQLTQFNNLAISDCVFW
                     IGSEVVEAPMRQGGAPVMHMISLRIVDRRDGRQLKVLYGLSNMLPTRIEPDLLGAWLV
                     VQSELGKGFQYAASFWRLLAQHEQQPALHKRALTAMQLGLAAWQIRQSDNARQVRAIV
                     SGLTGRSVPTAQAMASATLKLFTLKEMLA"
     gene            complement(3611..4123)
                     /locus_tag="VIR04_02550"
     CDS             complement(3611..4123)
                     /locus_tag="VIR04_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEY9459661.1"
                     /translation="METNRPSLNSQTATHILAQLQRSVQNELELIQKLVLVLQSEAQI
                     LAIPNDSAALNASTAIKNQLIEQLTHAVQTRANWLKQLGYGADQSALAAAAADDPALQ
                     ALCDDLISATRQANDLNMSNGIIIDTYIEHNQQAINTLRHLISSDSLYDASGRTKTLP
                     ANNKRNVKIA"
     gene            4292..4699
                     /gene="flgB"
                     /locus_tag="VIR04_02555"
     CDS             4292..4699
                     /gene="flgB"
                     /locus_tag="VIR04_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY9459662.1"
                     /translation="MIDRIGEDFNFYQKALSLRQQRQEVLASNIANSDTPNYKARDFD
                     FTSALQDAMGTSPRLPDTQLTLTSARHIPASAVSPDPAELLYRQPLQPSLDGNTVDMN
                     VERVQFADNTLRYQTDLSLVTQRIKTLMAAMQQ"
     gene            4726..>5379
                     /locus_tag="VIR04_02560"
     CDS             4726..>5379
                     /locus_tag="VIR04_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HEY9459663.1"
                     /translation="MGENKREWLMHFKQLKIKTSLSAMVLLLSALTLVAVALGWYSQQ
                     KTQSTLQGLIATGVSANSNVNSAYVNALLAVNQIDAAIRLDDINTRNLALGNADKSLE
                     ESRARLKTFLGAANLTGPDGAAVKQSLSETFESSWALALQLRALAEKQDIVGYETVEQ
                     SQAPKTAQAIETAFLKFDTLMRAQNGQTVQLIARDFAQSRYVYGSLVAIALLLACLAY
                     "
BASE COUNT         1099 a         1390 c         1631 g         1259 t
ORIGIN      
        1 taatgttcat taccgccggc atactggcgg cgatgggatt gattcccaat atgccgcatg
       61 tggcgttttt gtcgttggct ctgattttgg gtggcgccgg ctggttattg cagggacggc
      121 aacggatcga gcgcgcgcag ttggcgcaag aaccgcttaa aaatcaggca gccatcgaag
      181 cggccgccgc cgaggcgagt tgggatgatg tggccttggt cgaccccctg ggtctggagg
      241 tcggttatcg cctgatttcg ctggttgatc acgaacaaaa tggcgaactg ctgcatcgga
      301 tacgtggcct gcgcaagaaa ttcgcccagg atgtcggctt tttaccgccg gtggtgcata
      361 ttcgcgataa cctggaactc aaacctaatg actaccgcat attattgttt ggcgtcgagg
      421 ttggacatgg cgtggcgacg cccggccagt ggctggcgat tgatccgggt ggcgtgacca
      481 tgaaattgcc gggtgaggcg acaaccgatc cggcatttgg attgccggcg gtttggatca
      541 atgtctcact gcgcgaacag gctcagatcg ccggctacac cgtggtggac gcaggcactg
      601 tagtggcgac tcatttaaat catctgatgc atcgctacgg agccaacctt cttgggcgcc
      661 aggaagttca acaactgttg gaccacattg gcaaagaagc gcccaaactg gtggaagacc
      721 tggtgcctaa aacagtgacc ctgactctgt tgcagaagct gcttcgcggc ctgctggacg
      781 aggaagtggc cattcgcgat gtccgtacga ttattgaaac cctgaccgaa catgctccgc
      841 gactggccgc tttgaatccc aatgggagcg gaccggaccc cgccgagttg ctggcgcttg
      901 tccgtgttgc cctcggtcgc gccattgttc agcagtggtt ccctggcgac ggcgaattgc
      961 gcgttatcgg tctggatgcc aggctcgagc gtgtattgac ccaggcgctc accaccagcg
     1021 gcgtgctcga acctggcctg gctgaaaacg tggcgctcga cgcccagcgg gccgtgcagg
     1081 tccaggagag caacggtgat tcgcctgttc ttgtggttcc cgccgcattg cgtgtgtcgc
     1141 tttcacggtt tttgcggcat cacttgccgc aacttggggt actttccaat gctgagatac
     1201 ccgatgagcg catcttgcgt gtgacctgcg ttatcggtgg agataaagcg tgaacttaag
     1261 ccgttttttt ggcagtacca atcgcgaggt attgagacag gttcgtatgg cattagggcc
     1321 cgacgcgctt attgtgtcga atcgcagggt gaacggcggc gtggaaatta tggctgcgga
     1381 cgcgacatcc ctgcctgccg gagtgcaaga ccccgattcc aataaagtac gagaacccga
     1441 ctctaacgta atagaggcta ttggagcgat gcgcggcgcc cttgagacgc gcatcgacga
     1501 ggtgctatgg ggcagccagt tgcgacgcgc gcccgaggcg gtccaggtgt tccagactat
     1561 gttgggttgc ggattcagca cagcattgct gcgcgccatg ctcaagcgtt tgcccgcgca
     1621 tttgtcgccc ggggccgcgt ttcagtgggc ccgcaacgaa atcgtcactc acctgcccgt
     1681 attggaaagt gaaaatgatc tatgggcgcc agggctggcg ttggcgctcg tgggcccgac
     1741 gggtattggg aagacgacaa ccatagcgaa gcttgcggca cgttgtgcga aacgttttgg
     1801 tgcgcgaaac gtgaccttgt taaccaccga tacgtatcgg attggggcgc acgagcaact
     1861 gaagatctat ggccggatga tgcaactggc agttcacgtt gttcaagatg ccgacgagct
     1921 tcgccgcgtc gtgcaagaac tcgggccgga tcagattatc ctgattgaca atgtcggcgt
     1981 tagccagcgt gatcattata tttcgcagca agcgtctttg ctggcggcgg cggggcggcc
     2041 ggtcaagcga atactggtgg tgaatgcatc cagcaatggc gatacgctgg acgaggtggc
     2101 gcgttcttat tctaacgatg gcggtagccc attaacgggc tgcatcatta ccaagcttga
     2161 cgaagccacg cggttggcgg cggcgctgga tacggccatt cgctatcaat tgccgattca
     2221 ctatgtgtca agcgggcaaa aggtccccga ggatttatgg ttattatcgg cggcggacct
     2281 ggttgaccgg gcgctcgcag gaccgacgca gaccagaccg ttgtacgcgc caacccaggc
     2341 ggattttgca gccataatgt ctttggtaaa ggttccgcag cctgattcaa cggttgccga
     2401 tgaccgccgc aaacaactgt tgccaagcct gctttgcgcc gtcgggggcg ccaatttcgt
     2461 cctctccgag cccgagttga aggatgcctg cctttttttc gatgatgatc cggcttgttc
     2521 gaaagctttg gacctgtggc gcgattatgt cgcgccggtg tcgccggcgc cgacggattc
     2581 gccgattgaa aattccatcg aaaatatggt tcgctgcgcg cgaaattatc ttgccggcat
     2641 cgagcatcgc cacgttctgg caatacacga ttgcgtttcg ttaaaattgc gctccaacgc
     2701 gcacgggcat ttacgagcga cggcattgct tacggaacac gcacgagcgt tgacgtcccc
     2761 cattcagcaa ctcgcgttgg cggatggctg gcgatcttct tgcgggctga caagcctgga
     2821 ggcgccgcgc ccaagcgatg ctctgtatca tcaagtgcaa tggctcgctc agcgctgcga
     2881 tcggatgtca ttggtgcatg tgttcgggag cgccacgccg gcattgcggc gcaggctgtc
     2941 gctggaagga accgcctggc tgactcaatg tcccggcgcg accaaagtac agtttgactc
     3001 ctgtgccacc acgctgtccg cactggccaa gactcttgtc tttcgtccga tggaacagcc
     3061 agggcaaagc gtgcagctca cgcagttcaa caatctggcg atcagcgact gtgtgttctg
     3121 gattggctcc gaagttgttg aggcgccaat gcggcaaggt ggcgcgccgg tgatgcatat
     3181 gatttcgcta cggatcgtag accgtcgtga tggccgtcaa ttgaaggttt tgtacggatt
     3241 aagcaatatg cttcccacca gaatcgagcc cgatttgctg ggcgcctggc tggtcgtgca
     3301 gtcggagctg ggaaagggtt ttcaatacgc tgcgagtttc tggcgcctgc tggcgcagca
     3361 tgaacaacag ccagcgttgc ataaaagggc gctgacggcg atgcagcttg gattggcggc
     3421 atggcaaatc aggcagtcgg acaatgcgcg ccaagtgcgg gctattgtga gcggtttgac
     3481 cggccggtct gtgccgaccg cgcaagctat ggcaagcgcg acgctaaagt tgtttacgct
     3541 aaaggaaatg ctggcctagt gccgatcggg ctaattcgcg gtacgcgtaa ttcatgcgcg
     3601 gcttcaggcc ctacgcaatc ttgacgtttc ttttgttgtt ggcaggcagt gttttggtgc
     3661 ggccactggc atcgtacaag ctgtcgctac ttatcagatg gcgcagcgtg ttgatggcct
     3721 gttggttgtg ttcgatataa gtgtcgatga ttatgccgtt ggacatattc aagtcattgg
     3781 cctgtcgggt ggcgctgatc aggtcatcac acaaggcttg cagcgccgga tcgtccgctg
     3841 cggcggcggc caaagcggac tgatccgctc cgtaccccag ttgtttcaac cagttcgcgc
     3901 gagtttgcac tgcatgcgtt agttgttcga tcagttgatt tttaatcgcg gtggacgcgt
     3961 tcagtgccgc actgtcatta ggaatggcca ggatctgtgc ttcagattgc agcacgagca
     4021 cgagtttttg aatcagttcc agctcgtttt gaacgctacg ttgaagttgc gccagaatgt
     4081 gggttgccgt ctggctattc agtgatggtc ggttggtttc caaaatgatt tccgtaagac
     4141 atgtgactag gccgcgcttc gtcattttag aacgggctat tggtgcgcat gattggaact
     4201 gactgggaaa ttcaggctta tttcttagtt tatgcaaacg gccgcgcgct tactatgact
     4261 catgcttcca aatccattta tcgagttgcg gatgattgat cgtattggcg aggattttaa
     4321 tttttatcag aaggcattga gcttgcggca acagcgccag gaagtgctgg catcgaacat
     4381 tgccaattcc gatacgccaa attacaaggc gcgtgatttc gatttcactt cggccttgca
     4441 agacgctatg ggtacgagcc cacgcttgcc ggatactcag ttgacgctga catccgcacg
     4501 gcatattccc gctagcgcgg tttcgcctga tccggcggaa ttactgtatc gacagccgtt
     4561 gcagccaagt ctggatggca atacggtgga catgaatgtc gagcgggttc agttcgctga
     4621 caataccctg cgctatcaaa ctgacctgag ccttgtcacg caacggatca aaaccctgat
     4681 ggcggcaatg cagcagtagg gcgcgcaacg atatggcgct gcgcaatggg tgaaaacaag
     4741 cgagaatggt tgatgcattt caaacaatta aagattaaaa cgagcctgtc ggcaatggtg
     4801 cttttgttgt cggcgcttac tctggttgcc gtggctcttg gatggtatag ccagcaaaaa
     4861 acccaatcca ccctgcaggg cttgatcgcg acgggtgtct cggccaactc caatgttaat
     4921 agcgcgtatg taaacgcctt gctggctgtg aatcaaatcg atgccgccat acggctggat
     4981 gacataaaca cgcgcaatct tgcgttgggc aatgcggaca agtcgctgga agaatcgcgc
     5041 gcacgactca agacctttct gggcgccgcc aatttgaccg gcccggacgg cgctgccgtc
     5101 aagcagtcgc taagcgaaac gtttgaatca tcgtgggcct tggcgcttca gcttcgcgcg
     5161 ctggcggaaa agcaggatat cgtgggttat gaaacggtgg agcagagcca ggccccgaag
     5221 acggcgcagg cgattgaaac agcgtttcta aagttcgata cgcttatgcg agcgcagaac
     5281 ggccagacgg tgcagcttat cgccagggat ttcgcgcagt cgcgctatgt atatggaagt
     5341 cttgttgcaa tagcgttgtt gctggcttgt ctggcgtat
//
LOCUS       DATCXU010000117         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_591491,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000117 DATCXU010000000
VERSION     DATCXU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591491"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="bcsG"
                     /locus_tag="VIR04_02565"
     CDS             <1..1119
                     /gene="bcsG"
                     /locus_tag="VIR04_02565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HEY9459664.1"
                     /translation="SAVPVVRGGSSQTADGDYSAVIKRFREAEAPRHVSFERADPEAD
                     ARFDIIMVHICSLSWDDLKVAKAVDNPLLKHFDYLFTHFSGAVSYSGPAAIRLLRADC
                     GQTSHKELYKSAPQSCHIFSALQQEGYTAQIALNHDGRFDDFVGDVTRNIGVPGVKPM
                     SLEGVPMGLRAFDDSPIRDDYSTLARWWEARQKVEGPVALYYNTITLHDGNHMLDSKM
                     SSLESYPLRLTKLMSEIDRFAALVQSSGRKAVMVFIPEHGAALKGDAGQIAGLRDIPT
                     PKIINLPVGVRLINFAGTHGPTQVINAPSSYLALAQLLANMVANSPFKSSAPALDQYA
                     ANLPHTEMVGENENVITLPTPKGYVMRNAAGAWVTEYE"
     gene            1112..1282
                     /locus_tag="VIR04_02570"
     CDS             1112..1282
                     /locus_tag="VIR04_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459665.1"
                     /translation="MNDQQKWEISDIEGLHVHVPEVDPRLYFDQREYAALQRALITWP
                     ALARLMGLAPKS"
     gene            complement(1293..2675)
                     /locus_tag="VIR04_02575"
     CDS             complement(1293..2675)
                     /locus_tag="VIR04_02575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY9459666.1"
                     /translation="MTIPNRSRNNVVDFDLHQRLLTDLARTKDALREKETRLALAIEG
                     SGTGVWDRNVPTGEIFYSPGWKAILGYSDSEIGTRITDSYGRVHPDDLAFVQATIQDH
                     FDQKTDIYAVEHRIRCKDGSYKWISSRGKVVSRDADGKPLRMVGITTDITTLRTLSER
                     LQQSVDLITNLTNEVPGLVFQYHLLTDGTAFFSYASEGIRDIYELAPEQVANDASVMR
                     NIIHPKDYANYQASIAASANGLTPWHLEYRVILPKQGLCWRQGDARPRRLPDGSTVWH
                     GFISDITERKHAETELQELATIDFLTQLPNRRYFMARMEEGLAQIQRGYASSTAVLMC
                     DLDYFKSINDTYGHTTGDAVLKRFASILRKGLRKNDTVGRVGGEEFAVMLADAGLDEA
                     NLFAIRVQKHIANTTLTEGGATIPVTVSIGITIMKSSDASGATSLARSDIALYHAKDR
                     GRNRIEIAVE"
BASE COUNT          598 a          738 c          776 g          670 t
ORIGIN      
        1 tcggctgttc ctgtcgtgcg gggcggcagc tcgcagacgg ctgacgggga ttacagcgcg
       61 gtaatcaagc gttttcgcga agctgaagca ccgcggcacg tcagctttga acgcgccgac
      121 cctgaggccg acgcgcggtt cgacattatc atggtgcaca tatgctcact gtcctgggac
      181 gatcttaaag tcgccaaagc cgtcgataat ccgcttttga aacacttcga ttatcttttc
      241 acccacttca gcggggccgt cagctatagc gggccggctg ccatccggct gctgcgtgct
      301 gattgcggac aaacgtcgca taaagagttg tacaaatcag cgccgcagtc ctgccatata
      361 ttttcggctt tgcaacaaga gggttacacg gctcagattg cgttgaacca tgatggccgg
      421 tttgacgatt tcgtcggcga cgtcacgcgc aatataggcg ttcccggcgt aaagccgatg
      481 tcgctggaag gggtgcccat ggggctcagg gcctttgatg attctccaat tcgcgatgat
      541 tattcaacgc ttgcgcgctg gtgggaggcg cgccaaaagg ttgaggggcc ggtggcgctg
      601 tactacaaca cgattacttt gcacgatggc aatcatatgc tcgattcgaa aatgtccagc
      661 ctggagtcat acccgttgcg cctgaccaag ttgatgtccg aaatcgatcg atttgcggcg
      721 cttgttcagt catcgggacg caaagccgtg atggttttta ttcctgaaca tggcgcggcg
      781 ctcaaaggcg atgcgggaca aatcgcggga ctacgcgata ttcccacgcc taaaattatt
      841 aatttgccgg tcggcgtgcg cttgatcaat ttcgccggca cgcatgggcc gactcaggta
      901 atcaacgcgc caagcagcta tttggccctg gcgcagttgt tggccaatat ggtggccaat
      961 agcccattta aatccagtgc gcccgcattg gaccagtatg ccgccaattt gcctcacacg
     1021 gaaatggtcg gcgaaaacga gaacgtgatt acgctgccca cgcctaaagg atatgtgatg
     1081 cgcaatgcgg ccggagcctg ggtgactgag tatgaatgac cagcagaagt gggaaatcag
     1141 cgacattgaa gggctgcatg ttcatgtgcc agaagtcgat ccgcgccttt atttcgacca
     1201 gcgtgaatac gccgcgttgc aaagagccct gattacctgg cctgcactgg ccaggctaat
     1261 ggggcttgcg cccaaatcgt aaaccagcgt ttctattcaa cggcaatttc gatgcggttg
     1321 cgaccgcgat cttttgcatg gtagagcgcg atatcgctac gcgcaagcga agtggcgccg
     1381 ctcgcatcgc ttgatttcat gatggtaatg ccaatgctta cagtcactgg aatcgtcgcg
     1441 ccgccctcgg tcagagtagt attggcaatg tgtttttgaa ctcgaatggc aaagaggttt
     1501 gcctcatcaa gccccgcatc ggccagcatg acggcaaatt cttcgccacc cactcgaccg
     1561 actgtatcgt tcttgcgcaa ccccttgcgc aaaattgagg cgaaacgctt caggaccgcg
     1621 tcgcccgtgg tgtggccgta tgtgtcgttg atggatttga agtaatccag atcgcacatt
     1681 agtacggcgg tgctcgaggc atagccacgc tgaatttgag ccaacccttc ctccatccgc
     1741 gccatgaaat aacgccgatt gggcaattgc gtcaaaaaat cgatggtggc cagctcttgc
     1801 agttccgttt ctgcgtgttt gcgttcggtg atgtcgctaa taaagccgtg ccataccgtg
     1861 ctgccgtcgg gcagtcgtcg cgggcgtgcg tcgccctggc gccaacacag accctgcttg
     1921 ggcaatatga cgcggtattc aaggtgccac ggcgtcaggc cattggccga ggcggcaatg
     1981 gaagcttggt agttcgcgta atctttggga tgaataatgt tacgcataac ggaggcatca
     2041 ttggccacct gctctggtgc gagttcgtaa atatccctaa ttccttcgct ggcgtacgaa
     2101 aaaaaggctg tcccgtcagt caacaggtgg tattgaaata caaggccagg gacttcgttg
     2161 gttaaattgg ttatcaggtc gacgctctgc tgcagccgct cggataaagt gcgaagtgtc
     2221 gttatatcgg tagttatacc gaccatgcgc aatggtttgc catcggcgtc gcggctaacc
     2281 accttgccgc ggctcgatat ccatttatag ctgccgtcct tgcagcggat tcgatgctcc
     2341 actgcgtaaa tgtcagtctt ttgatcaaaa tggtcctgga ttgttgcctg aacgaaagcg
     2401 agatcgtccg gatgtacgcg accgtagctg tcagtgatcc gggtgccgat ttctgaatcg
     2461 gagtagccca aaatggcttt ccagccgggc gaataaaaga tttcacccgt tgggacgtta
     2521 cgatcccaga cacccgtccc gcttccctca attgccagtg cgagtcttgt ttctttttcg
     2581 cgcagcgcat ccttggttct tgccagatcc gtcagcagcc tctggtgcag atcgaaatct
     2641 acaacattgt tgcgggagcg gttcgggatc gtcatggctg ccggtattaa tagcgttaga
     2701 agcgatgaga caactgtacc ccaaccacgt cggcattggc tgtgtaaata ccatttatgg
     2761 ttccctggtt atttccgctg cc
//
LOCUS       DATCXU010000118         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1042666,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000118 DATCXU010000000
VERSION     DATCXU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042666"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /gene="rpoB"
                     /locus_tag="VIR04_02580"
     CDS             <1..1525
                     /gene="rpoB"
                     /locus_tag="VIR04_02580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465494.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY9459667.1"
                     /translation="AETQLNRLDDSGIVYIGAEVRADDVLVGKVTPKGETQLTPEEKL
                     LRAIFGEKASDVKDTSLRVPSGMVGTVIDVQVFTREGIERDKRAQSIIDDELRRYRQD
                     LNDQLRIVEEDMFARIEKYLVGKVVNGGPRKLAKGTALTKEYLADLDRWQWFDIRLAD
                     EDHAVALEQNKESLEQKRHQFDLAFEEKRKKLTQGDELPPGVLKMIKVYIAVKRRLQP
                     GDKMAGRHGNKGVVSRITSIEDMPHMADGTPADIVLNPLGVPSRMNVGQVLEVHLGWA
                     AKGVGLRIAEMFEDEKNIQAKSIRAFLEKVYNSSGTGAHLEVLSDDEVIEMARNLKDG
                     VPFATPVFDGATEEEIGKMLELAYPDEIAKRLQLTPSRTQAWLYDGRTGEQFERPVTV
                     GYMHYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAY
                     TLQEMLTVKSDDIAGRTKVYENIVKGDHVIDAGMPESFNVLVKEIRSLSLDMDLERK"
     gene            1526..>2827
                     /locus_tag="VIR04_02585"
     CDS             1526..>2827
                     /locus_tag="VIR04_02585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415725.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY9459668.1"
                     /translation="MKALLDLFKQVSQDEQFDAITIGIASPEKVRSWSYGEVRKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTVAKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAWCVIEPGMTPLKRGQIM
                     SDDDFLAKTEEYGDDFHALMGAEAVRELLRTIDINRDVETLRAELKATSSDAKIKKIS
                     KRLKVLEGFQKSGIKPEWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPDIILRNEKRMLQESVDSLLDNGRRGKAMTGANKRQLKSLADMIKGK
                     SGRFRQNLLGKRVDYSGRSVITVGPQLKLHQCGLPKLMALELFKPFIFNRLEMMGLAT
                     TIKAAKKLVESQEPVVWDILEEVIREHPIMLNRAPTLHRLGI"
BASE COUNT          683 a          724 c          825 g          595 t
ORIGIN      
        1 ggccgagacg caattgaacc gcctggatga ttcgggcatt gtgtatatcg gcgcggaagt
       61 gcgcgccgac gatgtgctgg taggcaaagt gacgccgaag ggcgaaacgc agttgacgcc
      121 ggaagaaaaa ctgttgcgcg ccattttcgg cgagaaagcc tccgatgtta aagacacatc
      181 gttgcgcgtt ccgtcaggca tggtcggcac ggttatcgac gttcaggttt tcacccgtga
      241 agggatcgaa cgcgacaagc gggctcaatc gatcatcgac gacgagttgc gccgctaccg
      301 gcaggacctt aacgatcaat tgcgcattgt cgaagaagac atgtttgcgc gtattgaaaa
      361 gtatctggtg ggtaaagtcg ttaacggcgg cccgcgcaag ctggccaagg gcacggcgct
      421 gacgaaagaa tacctggcgg accttgatcg ctggcagtgg ttcgatattc gcctggctga
      481 tgaagaccac gcggtggcgc tcgagcagaa taaggagtcg ctggaacaga aacgccatca
      541 gtttgatctt gccttcgagg aaaaacgcaa aaagctcacc cagggcgatg aactgccccc
      601 gggcgtgctg aagatgatca aggtgtatat cgccgtaaag cgccgcttgc agccgggcga
      661 caagatggcc ggccggcacg gcaacaaggg cgtggtgtcg cggattactt cgatcgaaga
      721 catgccgcac atggctgacg gcactcccgc cgacatcgta ttgaatccgt tgggcgtgcc
      781 ttcgcgcatg aacgtgggac aagtgcttga agtgcatttg ggctgggcgg ccaaaggcgt
      841 gggtttgcgg attgctgaaa tgttcgaaga cgaaaagaac attcaggcca aaagcatacg
      901 cgcgtttctt gagaaggttt acaacagcag cggcaccggt gcgcacctcg aggtacttag
      961 cgacgatgaa gtgattgaaa tggcgcgcaa tctgaaagat ggcgtaccgt ttgcaacacc
     1021 ggtatttgac ggcgcaaccg aggaagaaat aggcaaaatg ctggagcttg cctatcccga
     1081 tgaaatcgcg aagcgtttgc aactgacgcc cagccggacg caagcctggc tgtacgatgg
     1141 ccgcacgggt gagcaattcg agcgtcccgt taccgttggc tacatgcatt atctgaagtt
     1201 gcaccatttg gtcgacgaca agatgcatgc gcgttccaca gggccatact cgctggtcac
     1261 ccaacagccg ttgggcggca aggcgcagtt cggcggacaa cgttttggtg aaatggaagt
     1321 ctgggcgctc gaggcttatg gcgctgcata caccttgcag gaaatgctta ccgtcaagtc
     1381 tgacgatatc gccggtcgca ccaaagtcta cgagaacatc gttaaaggtg atcacgttat
     1441 tgatgctggt atgccagagt cgttcaacgt tctggttaaa gaaattagat ccttgtccct
     1501 ggacatggat ttggagcgta aataaatgaa agcgctactc gatctattta aacaagtctc
     1561 ccaagacgag caattcgatg cgatcacaat cggtatcgca tcgcctgaga aggtgcggtc
     1621 ctggtcttac ggcgaagtcc gcaagcccga gacaatcaac tatcgcactt tcaaacccga
     1681 gcgcgacggt ttgttttgcg ccaagatctt tggccctatc aaagattacg aatgcttgtg
     1741 cggcaaatac aaacggctga aacaccgtgg cgtcatttgc gaaaaatgcg gtgttgaagt
     1801 cacggtggcc aaagtgcgcc gcgagcgcat gggccatatc gaactggcaa gtccggtcgc
     1861 gcacatctgg ttcctgaaaa gtttgccatc ccgcctgggc atggtgcttg acatgacctt
     1921 gcgcgacatt gaacgcgtgc tttactttga agcatggtgc gtgattgaac ccggcatgac
     1981 tccgctaaag cgcggtcaga tcatgtccga cgacgatttt ctggccaaaa ccgaggaata
     2041 cggcgacgat ttccatgcac tgatgggtgc cgaagcggtg cgcgaactgt tgcgcaccat
     2101 cgatatcaac cgcgacgtgg aaaccttgcg cgccgagctc aaggccactt cgtccgacgc
     2161 caagatcaaa aaaatatcca agcgcctgaa ggtgctggaa gggttccaga aatccggcat
     2221 caagcctgaa tggatggtga tggaagtgct gcccgtgctt ccgccggact tgcgccctct
     2281 ggtgccgctt gatggcggcc gttttgcgac gtcggacttg aatgatttgt accgcagggt
     2341 catcaaccgc aacaatcgcc tgaaacgtct gctggaattg aaagcgcccg atattatttt
     2401 gcgcaatgaa aaacgcatgt tgcaggaatc ggttgattcg ctgctcgata acggtcgtcg
     2461 cggcaaagcc atgacgggcg ccaacaagcg ccagttaaaa tcgctggccg acatgatcaa
     2521 aggcaagagc ggccgcttcc gccagaactt gctgggcaag cgcgtcgatt attccggccg
     2581 gtcggttatt acagtgggtc ctcagctcaa gttgcatcaa tgcggtttgc ccaaattgat
     2641 ggcgctcgaa ctgttcaagc ctttcatttt caaccgtttg gaaatgatgg gtctggccac
     2701 gacaatcaag gccgccaaga agctggtcga gagccaggag cccgttgtat gggatattct
     2761 tgaggaagtc attcgcgaac atccgatcat gcttaaccgc gccccgaccc tgcaccgcct
     2821 gggtatt
//
LOCUS       DATCXU010000119         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_681902,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000119 DATCXU010000000
VERSION     DATCXU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681902"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VIR04_02590"
     CDS             <1..717
                     /locus_tag="VIR04_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9459669.1"
                     /translation="DKGQPLFDKGLSGGMLFDDAAFKAYAPNITPDPETGIGKWTDAQ
                     LAKAIREGIRPDGSLIGPPMPVPFYRNLSDADLSAIIAYLRAQPAVIHVVAKSSYNIP
                     LPPNYGPPVKHVSSPAMTNTLKYGEYLANIGHCMDCHTPRNNKGMLVTERLGAGGQRI
                     NWPGGAVVTPNLTVSDSGLKSWTNAQIAHAIRDGIDKDGARLKRVMAFDWYKNIDDSD
                     TNALIVYLRSLKPQPFGGKE"
     gene            748..1311
                     /locus_tag="VIR04_02595"
     CDS             748..1311
                     /locus_tag="VIR04_02595"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEY9459670.1"
                     /translation="MELRPPHYSHWRNAATPSFSTQQKYWLSRPGALTTGLRGLGPLE
                     LRVVREYAHGLLPCDAWMVAGVPRSPIWVREIIMSIDGVDSVFARSFTPLNASHGLWQ
                     GMRRLRTRPLADMLYNDPQIARSRFFSCRLTNQNPLYHCARNMLGAHCPPAHAMLARC
                     STFWRRGQPLLVAECFLPEFWPVAQRL"
     gene            complement(1329..1865)
                     /locus_tag="VIR04_02600"
     CDS             complement(1329..1865)
                     /locus_tag="VIR04_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY9459671.1"
                     /translation="MALRATIFKLDLHVADMDRNYYNSHTLTVARHPSETDERMMLRI
                     LAFALYADENLAFTRGLSTADEPSLWKKDLTGSILTWIELGHPDERRLLQASGKSRQV
                     VVLCYGGQASQVWWNGVQGKLTRVKNLSVLSVSPVAAKALAAMSQRSMDLHVSIEEQT
                     ALVSSPDDSVRMDVAVWR"
     gene            complement(1936..2658)
                     /locus_tag="VIR04_02605"
     CDS             complement(1936..2658)
                     /locus_tag="VIR04_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9459672.1"
                     /translation="MPIYKVKLKDRKEIANGTMMFRFEKPKGFVFKAGQFADYTIENP
                     SETDAEGNTRGFSLASAPYEDDLIAATRMRDTAFKRVLKTLPLGTELTLDAPYGSFTL
                     HNNAATPAVFLTGGIGVTPVRSMVLQALHDGADRKIFVFNSNKTPQDAAFLDDLSKPR
                     QEGPCYTFVNTMTEVKQSTNNHGDENGFITKAMLSKYVNDLTLPIYYIAGPQTMVAAM
                     RNILNEAGVDDDNIRTEEFSGY"
     gene            2952..3407
                     /locus_tag="VIR04_02610"
     CDS             2952..3407
                     /locus_tag="VIR04_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9459673.1"
                     /translation="MQNPTDKSEVIRLGQLEIRYLLSDNNSPSRLGSFEMTVPPNANV
                     PPPHRHANNDEFVYVLEGTLHYSVDDVSRDLGPGDSMFSPRGAVHAFSNPHSKAARAL
                     IIQTPDIGPQYFRDIAAIVNAGGPPDKAKLLGVMARYGLTLPDKVNAQG"
BASE COUNT          813 a         1054 c          905 g          761 t
ORIGIN      
        1 gacaaaggcc agccactatt cgataaaggt ctgtcgggcg gcatgctgtt cgacgacgcc
       61 gcgttcaaag cctatgcgcc gaacattacc cccgaccccg aaacgggcat cggcaaatgg
      121 accgatgcgc aacttgccaa agccatacgt gaaggcattc ggcctgacgg cagcctgatc
      181 ggcccaccca tgcccgttcc gttttatcgg aacttgtccg acgcggacct gtccgcgatc
      241 atcgcgtatc tgagagcaca gccagccgta atacatgtgg tggcgaagtc aagctacaac
      301 ataccgttgc cgcccaatta cgggccgccg gtgaagcacg ttagttctcc ggccatgaca
      361 aacaccttga aatacggtga ataccttgcc aacatcggtc actgcatgga ctgccacacg
      421 ccgcgcaata acaaaggcat gctggtgaca gagcgtctgg gcgctggagg ccagcggatc
      481 aactggccgg gcggcgcggt cgtcacgccc aacctgacgg tcagcgacag cgggctcaaa
      541 agctggacca acgcccaaat cgcacacgct atccgggacg gaatcgacaa ggacggcgca
      601 cgcctgaaac gcgtcatggc attcgactgg tacaagaata tcgacgactc ggacaccaac
      661 gcgctgattg tttatttgcg atcgctaaag cctcaaccat tcgggggcaa ggaataaggg
      721 agcggcgatt cggcgataat agggtttatg gaattacgac cgccccatta cagccactgg
      781 cgcaacgccg ccacaccctc gtttagcacg caacaaaaat actggttatc ccgccccgga
      841 gccttgacaa cgggtctgcg cggactcggc ccgcttgaat tgcgcgttgt ccgcgaatac
      901 gcccacggct tgctcccttg cgacgcatgg atggtggccg gggtgccgcg atcgccgatc
      961 tgggtacgcg aaatcattat gtccattgat ggcgtcgaca gcgtgtttgc ccgcagcttc
     1021 acaccattaa atgcttcgca tggcttgtgg cagggaatgc gccggctacg tacacggccg
     1081 ttggcggata tgctgtataa cgatccgcaa attgcacggt cgcgcttctt ttcctgtcgc
     1141 ctgacgaacc aaaacccctt gtatcattgc gcccgcaata tgcttggtgc acattgccct
     1201 cccgcgcatg ccatgctggc acgctgttca acgttctggc ggcgcggaca gccactgctc
     1261 gttgcagaat gcttcttgcc cgagttctgg ccggtggccc aacgcctata acgcagcgcg
     1321 ccgtcaggct aacgccacac ggccacatcc atccgcacac tatcatccgg tgacgaaaca
     1381 agcgccgttt gttcctcgat ggagacatgc aaatccatcg agcgttgcga catcgccgcc
     1441 aatgccttcg cggcaacggg gctgaccgat aacacgctca gatttttcac acgagttaac
     1501 ttgccctgca cgccgttcca ccacacctgg ctagcctgac caccatagca aagcaccacc
     1561 acctgccgcg atttgccact ggcctgcaac agacgccgct catcggggtg tcccagttcg
     1621 atccatgtca ggatggagcc ggtcaaatcc tttttccaca gtgacggttc atcggccgtg
     1681 ctcagtccgc gggtaaacgc caaattctca tccgcgtaca gggcaaacgc caatatgcgc
     1741 agcatcatgc gttcgtccgt ttcagaagga tgacgggcca cggtgagagt atgactgttg
     1801 tagtagttgc gatccatatc ggccacatgc aagtcgagct tgaaaatcgt ggcgcgcaat
     1861 gccataagca tccttaaaaa aagattccgc ctgcacgcag acggaatcgt tgccgacgtc
     1921 gaagtcgtgt aacgcttaat aacccgagaa ttcctcggtg cgaatgttgt cgtcatccac
     1981 cccggcttcg ttcagaatgt ttcgcattgc ggctaccatc gtctgcgggc cggcgatgta
     2041 ataaatgggc aaagtcaaat cgttgacgta ttttgaaagc atcgctttgg taatgaaccc
     2101 attttcatca ccgtgattgt tcgtcgattg ctttacctcg gtcatggtgt tgacaaatgt
     2161 atagcaaggg ccttcttggc gtggcttgga cagatcatct aaaaaggccg catcctgcgg
     2221 agttttattt gaattgaaaa cgaatatttt gcgatcggct ccatcatgca gggcctgcaa
     2281 caccatgctt cgaacggggg tcacgccgat accgccagta aggaatacgg cgggggtggc
     2341 cgcattattg tgcaacgtga acgatccgta cggagcatcc agcgtcagtt ccgtacccaa
     2401 cggcagggtt ttcaaaaccc gcttgaaagc cgtgtcgcgc atgcgcgtgg ctgcgattaa
     2461 atcatcttcg taaggggcgc ttgccaaaga aaagccgcgt gtattgcctt cggcatcggt
     2521 ttctgacggg ttttcaatgg tgtaatcggc gaactgccct gccttaaaaa caaacccctt
     2581 tggtttttca aagcgaaaca tcatcgttcc gttggcaatt tcttttctgt ctttcaactt
     2641 aactttgtaa atcggcataa cctgtttctc ctggtcctat ctattaatcc atcaaaggcc
     2701 gcccagccct ccccttatta gagctggcat tacttgcgca cgttcctggt atttgcgccg
     2761 ccaagcctcc ttcaatcaga atgataagca gtataccgcc ctgcaatgat gccgccggcc
     2821 ctcggtggat acagccattc gcaagatctt cgcgaacggc agcgcccccg ccttcctgac
     2881 tattgacccc tccaaaataa aacttacaat taaatccagg taaacatatt tacacctgaa
     2941 ggaaggcgct catgcaaaac cctactgata aatcagaggt cattcggctt ggtcaattgg
     3001 aaattcgcta cttgctgagc gataacaaca gccccagccg tcttggctca tttgagatga
     3061 ctgtgccgcc caacgctaac gtgcctccgc cgcaccgaca cgcgaataac gacgaattcg
     3121 tctatgtgct tgaaggcacg ctgcactatt cagtcgacga tgtttcgcgc gacctggggc
     3181 cgggcgattc gatgttcagt ccacgcggcg cggtgcatgc cttcagcaat ccacacagta
     3241 aagccgcgcg agccctgatc atacaaaccc cggatatcgg cccgcagtac tttcgcgaca
     3301 tcgccgccat tgtcaacgcc ggcggaccac ccgacaaggc caaactgctg ggggtcatgg
     3361 cgcgctatgg actgacgctg ccagacaagg tcaacgcgca aggttaacac gccgcctttc
     3421 gaagcacgat ttttacacca tcaggcgtca gtgctttgtg gaggtagcgc cgttcccggc
     3481 aagaatcatc cacggcacac cgaagcgatc ggccaccatg ccaaagcttg acg
//
LOCUS       DATCXU010000120         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_177524,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000120 DATCXU010000000
VERSION     DATCXU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177524"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VIR04_02615"
     CDS             complement(<1..1069)
                     /locus_tag="VIR04_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9459674.1"
                     /translation="MNTKTNRTSITLPAQEVPLLLEADVVVVGGGTTGPFAAISAARR
                     GKKVVMIERFGSLGGNLTLGLNTKPSGALVGGLPLEVWNLARSVNGAGEDYMAISKTG
                     GVKIASPCDPELMKILLTRLCVESGVQILFETLVSCPIIEDGAITGVVVESKAGRQFI
                     SAKVVIDCSADADIAAQANVPFVMGAGDTEVTMQPVSMYYTMTKVDIGRLAKWARTCD
                     DIPARAIPDTEEGLQYGLWLTGFNGMLKKFEAESGLKLQRDNITLKTANGLMYINATR
                     VLGTDVFSPVDFTAAIVECYRQIEAVTQFLQTRVPGFENAQIGQIAPVLGVRETRHIC
                     GEYTLTGADALDGKRFDDSIAA"
     gene            complement(1215..1814)
                     /locus_tag="VIR04_02620"
     CDS             complement(1215..1814)
                     /locus_tag="VIR04_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY9459675.1"
                     /translation="MAVSVDYYFSVASPWTYLGSARFISMVRRSHATVRVMPLDYTRV
                     FAATGGLLYQHRAPARRAYRQVELARWRDTLGIHLQLEPCFYPVDRRPASCMLIAARD
                     QEQAAILELSHAILRTAWCDDGDIADWDTLVRLADQVGLDGRVLAGRARDPAIMKQYE
                     QDTEDAIAIQVFGAPSYVIDGEIFWGQDRLDFVERKLGL"
     gene            complement(1851..>2050)
                     /locus_tag="VIR04_02625"
     CDS             complement(1851..>2050)
                     /locus_tag="VIR04_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9459676.1"
                     /translation="RCLSATSYAHGATRNMAPCMVTGEAAGVAAALCAQAGTPCADLP
                     IDLLQSRLLESGVYLGETITV"
BASE COUNT          448 a          623 c          554 g          425 t
ORIGIN      
        1 ccgctgcgat actgtcgtca aaacgcttgc cgtcaagcgc gtccgcgcct gtcaaggtat
       61 attcgccgca aatgtggcgc gtttcgcgca ccccaagcac cggggcgatt tgaccgattt
      121 gtgcgttctc gaagccaggc acgcgagtct gcaggaactg ggtgacagct tcgatctggc
      181 gataacactc cacgatggcc gcggtgaaat ctactgggga aaaaacgtcg gtgcccaaaa
      241 cacgcgtggc attgatatac atcaagccgt tggctgtttt tagcgtgatg ttgtcgcgct
      301 gcagtttcag gccggactcg gcttcgaatt ttttcaacat cccgttaaat ccggttagcc
      361 acaatccgta ctgcaaccct tcctcggtgt cggggatcgc gcgggcggga atatcgtcac
      421 aggtacgcgc ccacttggcc agccggccaa tatcaacctt ggtcattgtg tagtacatgg
      481 ataccggctg catcgtgacc tcggtatcgc cagcccccat aacgaatggc acattggcct
      541 gcgcggcaat atcggcgtct gccgagcaat cgatgacgac cttggcactt atgaattgac
      601 ggcccgcctt gctttcaacg acgacgccgg ttattgctcc gtcttcaatg atgggacatg
      661 aaaccagggt ctcaaacagt atttgcacgc ccgattccac gcacagtctg gtaagcagga
      721 tcttcatcaa ttcgggatcg cagggcgatg cgattttgac cccgcccgtc ttggaaatcg
      781 ccatatagtc ttcgccggcg ccattcaccg agcgggccaa attccagact tcaagcggca
      841 gaccgcctac cagggcacct gacggcttgg tattcaatcc aagcgtcagg ttgcccccca
      901 gcgagccgaa ccgctcgatc atgaccactt tctttccgcg gcgagccgcc gaaatggccg
      961 cgaaagggcc cgtggtgcca ccgccgacga ccacgacatc ggcctccagc aatagcggaa
     1021 cttcctgcgc cggcaaggta atcgaagttc ggttggtttt tgtattcatt gacccgctcc
     1081 atgaaaattc ataattgggc cgacgtgcaa cggcaacccg ttaaacgttt cactgatgaa
     1141 acgttgtttc aacgtataat catcctattt tcccgactat tcgtcaagta ataccggatc
     1201 gccacgacgc gcggtcacaa tcccaatttg cgctcgacga aatcaaggcg gtcctggccc
     1261 caaaatattt cgccatcgat cacgtagctg ggcgcgccaa aaacctgaat cgcaatcgcg
     1321 tcctcggtgt cttgctcata ttgcttcatg attgccgggt cgcgggctcg tccagccagc
     1381 acacgcccgt ccaggccgac ctggtccgcc aggcgcacga gcgtatccca atcggcaata
     1441 tcgccatcgt cgcaccatgc agtacgaagt attgcgtgcg acaactccag gatggccgct
     1501 tgttcttgat cccgcgcggc gatcaacata cagcttgcag gccgacgatc cacggggtag
     1561 aagcacggtt cgagctgtaa atgaatcccc aatgtgtcgc gccatcgcgc cagttcgacc
     1621 tggcgatacg cacgccgcgc cggtgcccga tgttgataga gcaatccgcc tgtggcggca
     1681 aacacacggg tgtaatccag tggcattact ctgaccgtgg catggctgcg tcgcaccatc
     1741 gaaatgaatc gcgcgcttcc aagataagtc cacgggctgg ccacactaaa gtagtaatcg
     1801 acggatacgg ccatgacaat aattccccga cagtgacagg ccaaaggttc tcaaacggtg
     1861 atcgtctcac cgaggtatac gcccgattcg agcaatcgtg attgaagcag gtcgataggg
     1921 aggtccgcgc agggtgtgcc ggcctgggcg cacaacgcgg cggcaacgcc ggccgcttcg
     1981 ccggtcacca tacagggagc catgttgcgg gtggccccat gcgcatacga tgtcgccgac
     2041 aagcagcgcc
//
LOCUS       DATCXU010000121         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1121690,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000121 DATCXU010000000
VERSION     DATCXU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121690"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VIR04_02630"
     CDS             <1..360
                     /locus_tag="VIR04_02630"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9459677.1"
                     /translation="GEEEKKILPAVKSQQALGRLVEGPLPADTLFFRAGRGDFDLVVA
                     MYHDQGHGPIKVLGLEAGVNITVGLPIIRTSVDHGTAFDIAGKGIADERSLLEALRAA
                     IELSPKKATTISDRQPA"
     gene            complement(463..1263)
                     /locus_tag="VIR04_02635"
     CDS             complement(463..1263)
                     /locus_tag="VIR04_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9459678.1"
                     /translation="MLSKKVAILALGAFSLAASLAPVSADTLQTIQQRGKIIVGVDIG
                     APPYGMLDKNAKQTGFDIDAAHSLAKDLGVQLQVVPVTGPTRVQFLRTNKVDVIMASF
                     SITPERKKVIDFSDPYAVVPIVVGGPKADKVSSAKDLAGKTVAVTRGTTQDQAITKAA
                     KEVPTINIVRYEDDATTSTAVLTGQQNYIIAASSVLPVLHESNPDRDIAYKYTAVSFP
                     MGVGLRQNEPQLKDRVNQWVSKNLKNGTLNETYKKYFFGQSLPAEMLK"
     gene            complement(1307..2035)
                     /locus_tag="VIR04_02640"
     CDS             complement(1307..2035)
                     /locus_tag="VIR04_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9459679.1"
                     /translation="MDPVVSFKNVKKSYGRDEVLKGISFEVFEGGVVALIGQSGSGKS
                     TALRCINGLESINGGELRVCGRDLHKKECDIFSLRKEVGIVFQSYNLFPHLSVEQNIT
                     IAPRKVKKIKTREAHELALHVLNQVGLVDKAAAYPEQLSGGQQQRVAIARSLAMQPKL
                     MLFDEVTSALDPQITGEVLKVIASLAKDGMTMVLVTHEMAFAQAVADQILYVRNGVVW
                     EAGDAGLLKNPQTPELRQFISNGL"
     gene            complement(2050..>2362)
                     /locus_tag="VIR04_02645"
     CDS             complement(2050..>2362)
                     /locus_tag="VIR04_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9459680.1"
                     /translation="TQWEASHSLAFNGLQTLAYIILPQAFRVAIPPTVGFLVQIIKNS
                     SYAVVIGFFDLTYSARVVNNSTFKPFAVFTVAALIYFILCYPLSLWSYRLEKKLKDKT
                     N"
BASE COUNT          560 a          644 c          596 g          562 t
ORIGIN      
        1 ggcgaagaag aaaagaaaat cttgccggcc gtaaagtcgc aacaagcgct ggggcgttta
       61 gtcgaaggcc ccctgcccgc cgatacgctt tttttccgcg cggggcgtgg cgattttgat
      121 ctggttgttg cgatgtatca cgaccagggt catgggccca tcaaggtgct gggtctggag
      181 gccggcgtaa acattacagt cggactgccg atcattcgca catctgtaga ccatgggaca
      241 gcgtttgata ttgcgggcaa gggcatcgcc gacgagcgca gtttgctgga agcgctgcgc
      301 gccgccatag agctttcacc gaagaaagcg accacaataa gcgatcgcca gccggcatag
      361 ccatacgcgc caatacagac agcgggaatt cgacaagcca ggcgcgaagg tcgcgcactg
      421 gacttgatgc gaagtcccgc tattggaata cgactcttgt cgttatttca acatttccgc
      481 aggcaaggac tgcccgaaga agtacttttt atacgtctcg ttaagcgtcc cgtttttcag
      541 gtttttcgag acccactgat tgaccctgtc ctttaactgc ggctcattct gacgaagccc
      601 aactcccatg ggaaagctga ctgccgtgta tttgtacgca atgtcgcgat caggattgga
      661 ttcgtgcaaa accggcaaga cactactggc cgcgatgata tagttttgct ggccggtcaa
      721 aactgccgtg cttgtcgtgg catcatcttc atagcgaacg atatttatag tggggacttc
      781 cttcgcggct ttggtaatag cctggtcctg cgtagtgccg cgcgtgacgg caaccgtttt
      841 gccagccagg tcctttgccg aagagacctt gtccgccttc gggccgccca ccacaatggg
      901 aacaaccgca tacgggtccg agaaatctat gaccttcttt ctttcaggcg tgatcgaaaa
      961 cgaagccatg attacatcga ctttgttggt gcgcaggaat tgcacacgcg tcggtccagt
     1021 gaccggcaca acctgaagct ggacgcccaa atccttggcc aatgaatggg cggcatcgat
     1081 atcgaagccg gtctgcttcg cgtttttgtc gagcatgccg tacggaggcg cgccaatgtc
     1141 gacgcctacg atgatcttgc ccctttgctg gatcgtctga agcgtatccg cgctaaccgg
     1201 cgcaagcgat gccgccaatg aaaaagcgcc aagcgccaat attgcgactt ttttactcaa
     1261 catggttatc tccaaaaaat aatcaatggt tggtgcaaca ttaaacttaa agaccgttgc
     1321 ttatgaactg ccttaattca ggcgtttgag gatttttaag caagccggca tcgcccgctt
     1381 cccatacgac gccatttctg acataaagaa tttgatccgc gacagcttgt gcgaaagcca
     1441 tctcgtgggt cacaagcacc atggtcatgc catctttggc caaacttgcg atcactttca
     1501 gaacctcgcc ggttatctgc ggatcgagcg ccgacgtgac ctcgtcaaat aacatgagtt
     1561 tcggctgcat ggccaacgaa cgtgcaatcg ccacacgctg ttgctgtccg cctgaaagct
     1621 gttcgggata cgctgcggcc ttgtccacca ggcccacctg attcaacaca tgcaacgcca
     1681 gctcgtgcgc ttcgcgcgtt ttgatttttt ttaccttgcg cggtgcaatc gttatgtttt
     1741 gctcgacgct taaatgcggg aacaggttat agctttggaa aacaatcccc acttccttgc
     1801 gcagactaaa aatgtcgcac tcttttttgt gcaagtctct gccgcaaaca cgcaactcgc
     1861 cgccattgat gctttcaagg ccattgatac atcgaagggc cgtgcttttt cccgagccgc
     1921 tttgcccgat cagagcgacg accccgcctt cgaacacctc gaatgaaata ccctttaaaa
     1981 cctcgtcccg gccataggat ttcttgacat ttttaaagct gacgacagga tccataacaa
     2041 cacagccctt taattggttt tatccttcag ctttttttcc aagcgatacg accacaagga
     2101 aagcggatag cacagtatga aataaataag cgccgctacg gtaaataccg caaaaggctt
     2161 gaatgtggag ttgttgacga ccctggccga ataggtcaga tcgaagaagc caatcaccac
     2221 cgcgtaggaa gagttcttga taatctggac caggaacccc accgtgggcg gaatcgccac
     2281 cctgaatgct tgcggcaaaa taatgtacgc cagtgtttgc aggccgttga aagccagact
     2341 atgcgaggct tcccactgcg tg
//
LOCUS       DATCXU010000122         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_187064,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000122 DATCXU010000000
VERSION     DATCXU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187064"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..867)
                     /locus_tag="VIR04_02650"
     CDS             complement(133..867)
                     /locus_tag="VIR04_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9459681.1"
                     /translation="MDAIKRHSVTTPADTPPSGDVIKTAGDTQPTVQRTVEILEEDIV
                     FGYLHPRERLTEDDLRARFDLKRHVVRQVLSELEQMGLVERKKNIGAFVKSYDAKEVT
                     DLYTVREILETSAAQHIPFPVPAERLDALIAVQKQHDRAVAESNLRVAFRANIAFHKL
                     LFALTGNPALTGVIEDFAQRTHIVRSLSMIVPHLLEKARLDHWMIIDALRAADSERLV
                     AICKDHLRPSRDAYIDQARQRENRRL"
     gene            complement(877..1803)
                     /locus_tag="VIR04_02655"
     CDS             complement(877..1803)
                     /locus_tag="VIR04_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY9459682.1"
                     /translation="MNESKTAVIIGGGIMGSDIAAIFFAAGWNTQVVETNPAARDRLE
                     TTVPAAARVAGVDHAGGSVRPLASLSEIDWAGVRLVIECVSENLELKQKVFSTLETLA
                     PADIPLTSNSSSFPISQIALHCSSAHRMLGLHFFMPAHLVPAVEVIRSAQSDEAVAQS
                     VSAIMRSVHKKPVQVKRDIPGFLANRIQHAMMREAIALVDEGFATAEDVDTAVRYGFG
                     FRYLAAGPLLQKDLAGIDIHCAAATTIYPDLHNNDRPSAIMRDLVAAGNFGVKSKNRK
                     GFYDWDDASIAREQKRYQTALTKAMQILRDEE"
     gene            complement(1835..>2848)
                     /locus_tag="VIR04_02660"
     CDS             complement(1835..>2848)
                     /locus_tag="VIR04_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY9459683.1"
                     /translation="VATADGQEGIGFCYGGSRGGILVTHAVRELYKEMLIGQDALRVE
                     GLWQDMYQDTLLHGRAGSAMRALSILDIAMWDRNARAAGLPLSKYLGGYTKQKVPAYA
                     SGGYYLEGKTPEKLGEELKGYVEQGFKAVKIKVGRLSLREEEIRIKTAREAIGDDILL
                     MLDANNAWHDVPTAVEYMKRYEPSNPYWIEEPFSPDDINSHAELARRTFISVATGEIE
                     AGRWRHQELLQKHAAAILQSDAAVCGGISEWRRIAAAAAGFGVNLCPHWFHDLHVHLV
                     AATPNAQFVEFFPNDQVLNFRRLIDTQLVVEDGDLVVPTGPGLGFRFDEKALEKYAIE
                     KWA"
BASE COUNT          570 a          803 c          811 g          664 t
ORIGIN      
        1 acaaaacact ttatattacg gaatccgaat caggctcgat tctgatggcg cagctcgatg
       61 tgccgggccg cccaatgtac tcgcatcttt aatgcatgca ccacgattca tttacgtaaa
      121 ctaaaaggga gatcatagtc gccggttttc cctttgccgg gcctgatcaa tataggcgtc
      181 gcgcgacggg cgcagatgat ctttgcagat ggcgaccagc cgttcgctat cggccgcgcg
      241 cagcgcgtcg attatcatcc agtggtccag acgggccttt tcgagcagat gtggcacgat
      301 catggatagg gaacgaacaa tgtgggtgcg ctgagcaaag tcttcgatga caccggtcaa
      361 agccgggttg ccggtcagtg caaacaacaa cttgtgaaag gcaatatttg cgcgaaaggc
      421 aactcgcagg ttcgattcgg caacggcgcg gtcgtgttgc ttttgcactg caatcagcgc
      481 atccagacgt tcggcaggga cagggaacgg gatatgttgc gctgcgcttg tttcaaggat
      541 ctcgcgtacc gtgtacaggt ccgtcacctc tttcgcgtcg taggacttta cgaatgcgcc
      601 gatgttcttt ttacgttcca ccaggcccat ttgctcgagc tcggaaagca cttggcgcac
      661 gacgtgccgt ttcaagtcaa accgcgcacg caagtcatct tcggtcagcc gctcgcgagg
      721 atgcaagtaa ccgaagacga tgtcctcttc gaggatttca accgtacgct gtacggttgg
      781 ttgcgtgtcg ccagccgtct tgatcacatc gcccgatggg ggtgtgtcgg ctggcgtcgt
      841 tacggaatgc cttttgatag cgtccatggg cgaattttac tcttcgtcgc gtaaaatctg
      901 catcgctttt gtcaaggcgg tctggtagcg tttttgctcg cgtgcaatgg acgcgtcatc
      961 ccagtcatag aagcctttgc ggtttttgga cttgacgccg aaattacccg ccgccacgag
     1021 gtcgcgcatg atggcgctgg ggcgatcgtt gttgtgcaag tcgggataaa tcgtggtggc
     1081 ggccgcacaa tgaatgtcta tccccgccaa atccttctgc agcaaggggc ctgcggccaa
     1141 gtagcgaaac ccgaacccgt agcgcacggc agtatcgacg tcctcggcgg tggcgaaacc
     1201 ctcgtccacc agcgcgatgg cctcgcgcat catggcatgc tgaattcggt tggccaaaaa
     1261 ccccggaatg tcgcgtttga cctgcactgg ctttttatgc accgagcgca tgattgcact
     1321 tacagattgc gcaaccgctt cgtctgattg agcgctgcgt atgacctcga cagcgggcac
     1381 caaatgggcg ggcatgaaaa agtgcagacc gagcattcgg tgcgcgctcg agcaatgcaa
     1441 cgctatttgg ctgatgggaa agctggatga gttgctggtc agcggaatgt cggcgggcgc
     1501 cagcgtttca agcgttgaaa aaactttttg cttgagttcc agattttcgg atacgcattc
     1561 gatcaccagc cgtacaccgg cccagtctat ttcgcttagg ctggccaagg gccgtacaga
     1621 accccctgcg tggtcgacgc ccgccacccg ggcggctgcc ggaacagtcg tttcaagccg
     1681 gtcacgggcg gccggattgg tttcgaccac ctgtgtgttc catcctgccg caaaaaaaat
     1741 ggcggcgatg tccgagccca taatgccgcc gccaataatg acggctgttt tgctttcgtt
     1801 caaaggaatc tccatggatg tgtattaatg acggtcaggc ccatttttcg atcgcgtatt
     1861 tttcaagcgc cttttcatcg aaccggaatc caagtccggg cccggtgggc accacaaggt
     1921 ctccgtcttc aacaaccagt tgagtgtcga tcaggcgccg gaaattgaga acctggtcgt
     1981 tggggaagaa ctcgacgaac tgggcgttgg gcgtggcggc gaccagatgg acgtgcagat
     2041 cgtggaacca gtgcgggcaa agattgacac cgaagccggc tgcggccgcg gcgattcggc
     2101 gccactcgct gatgccgcca caaaccgccg catccgattg caaaattgcc gccgcgtgtt
     2161 tttgcagcag ttcctggtgg cgccagcgtc cagcctcgat ttcgccggtg gccactgata
     2221 tgaatgttcg tcttgcgagc tcggcatggc tgttgatgtc atcggggctg aacggctctt
     2281 cgatccagta cggattggat ggctcatagc gcttcatgta ttccacggca gtcggtacgt
     2341 cgtgccatgc gttgttcgcg tccagcatca gcaaaatgtc gtcgccaatc gcttctcgcg
     2401 cggtctttat gcgaatttcc tcttcgcgca ggctgagtcg tccgacttta attttcactg
     2461 ccttgaagcc ctgttcaaca tagcccttta gttcttcgcc cagtttttcg ggcgtcttgc
     2521 cttcgaggta gtagccgccg ctggcgtaag cgggaacctt ctgtttcgtg tagccgccca
     2581 agtatttact gagaggcaat cctgcggcgc gcgcattgcg atcccacatg gcgatgtcca
     2641 gaatagacag cgcgcgcatg gctgaaccag ccctgccgtg caacaatgtg tcctggtaca
     2701 tgtcttgcca caatccctcg acgcgaagcg catcctggcc gatcagcatt tctttataca
     2761 gttcgcgcac tgcatgtgtg accagaatgc cgccccggct gcccccgtag caaaagccga
     2821 taccttcctg accgtcggcg gtcgccac
//
LOCUS       DATCXU010000123         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_658920,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000123 DATCXU010000000
VERSION     DATCXU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658920"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VIR04_02665"
     CDS             <1..961
                     /locus_tag="VIR04_02665"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY9459684.1"
                     /translation="AAIALHAKKTGGLMTQADLAAHRTDWVEPIRMAYRDIELVEIGP
                     NGQGIGALMALGILDAFSLAGTEVDSAESVHLQIEATKLAFADMHAYVGDIDAMTTVR
                     TEDLLDRGYLTARARNIDPRRATYHGPGKPHHGGTVYLTAADRNGMMVSFIQSCFKGF
                     GSGVVVPDTGIALQNRGWGFSTRAGHPNQVGPGKRPFHTIIPGFLTRQGQPLMTFGVM
                     GGSIQAQGHVQVTSRIADYGQNPQAANDGPRWRIMDDNRTVKVEWNMPEKTIQGLREK
                     GHDVQVSPRFDLEFGCAQMALKLPAGGYFAASDQRKDGYAVGF"
     gene            1004..1777
                     /gene="ppk2"
                     /locus_tag="VIR04_02670"
     CDS             1004..1777
                     /gene="ppk2"
                     /locus_tag="VIR04_02670"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY9459685.1"
                     /translation="MSSNSVSARDYKTQLRLLQIELVKLQRHIISAGDKVLVLLEGRD
                     AAGKDGSIKRIIAHLSPRETRVVALGKPSSRDRSTWYFQRYVAHLPAAEEFVLFNRSW
                     YNRAGVEHVLGFCSDDEYEEFMQSAPKFEEMLVHSGIKLMKYYLDINKEEQKRRLLDR
                     QRDPLTQWKISPIDAVAVKHWKAYSEARDEMLLRTHTKVAPWRIVRADDKRLARLNLM
                     RDILTRLRYDGKKNKLVRPDTDVVFEFAPECISGKRLAR"
     gene            1889..2077
                     /locus_tag="VIR04_02675"
     CDS             1889..2077
                     /locus_tag="VIR04_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEY9459686.1"
                     /translation="MPAKSQAQQKAAGAALSAKRGETKLAKLKGSSKSMYKSMSEKEL
                     KELASTPRKGKPKHKSDS"
     gene            complement(2099..2578)
                     /locus_tag="VIR04_02680"
     CDS             complement(2099..2578)
                     /locus_tag="VIR04_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY9459687.1"
                     /translation="MKKILIISACFLPFVIAPAAYAQTGQPATPGTAPAPGAPGTPAA
                     PGAPGMPGGMPGSRTMPTPAAPPAQTISGWSVKHKVMGKTVFNEKDVKIGDIDDVVLT
                     PDGKPATLVVGAGGFLGMGAHDVAIPFDKIKERNNKLVLEGYTKDQLKALPEVKVMK"
     gene            complement(2652..2798)
                     /locus_tag="VIR04_02685"
     CDS             complement(2652..2798)
                     /locus_tag="VIR04_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY9459688.1"
                     /translation="MLYTIAVILVILWLLGLVTSYTLGGFIHILIVIAIVMVLIRLIS
                     GRAP"
     gene            complement(2849..3064)
                     /locus_tag="VIR04_02690"
     CDS             complement(2849..3064)
                     /locus_tag="VIR04_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY9459689.1"
                     /translation="MKTIKRATVCALTATVLLSLSACSGMSRQGRNTAVGAGVGAVGG
                     AVLTGGSTLGTLGGAAVGGVVGHEATK"
     gene            3215..3565
                     /locus_tag="VIR04_02695"
     CDS             3215..3565
                     /locus_tag="VIR04_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY9459690.1"
                     /translation="MITQKRMLVPMMMAVFALTACVAQPYGVAPPATSYYRGTTGNMI
                     PATNDASINANVVAAVQRSSGGVTNLQVTTLNGVVTLRGTADSRLVAENDVQAARQVP
                     GVQRVDYDIQILGR"
     gene            3788..5131
                     /locus_tag="VIR04_02700"
     CDS             3788..5131
                     /locus_tag="VIR04_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459691.1"
                     /translation="MSDQNSSPMSRRKFFINVGAVGGAVVLSSAFPNKTFAAAPPAIN
                     LGQIAPMTGAAAEFGPYYRDAVQLAVDQINKAAQEVFGGPIIAKHVTVDTTTVPTVGV
                     QAARQMVDTDKVPAIVGGWSSGVTVAVATSVTIPSGVLQVANGATSPLITVLPADAKS
                     DLLFRTTASDALQGVVAAQLINGEIIKDYKFKSVSTIYINNPYGQGLSNSFAKSFEHR
                     GGKVFAQVPHPEQVQPTYKSELALALKNKPDLLLAASYPSHTAVFLKESRDIFNFTSW
                     ELTDGNASLDVLTAVGAKNMDGKMGTAPGEDTTTAAYKDFAKNFLAAYKYDHIPPFTA
                     STYDAAMTIGLALAKVIANGETNASKITGGALRDQLRVVSNPPGDEIEGGDQGRITEM
                     LKMIKAGKKINYTGAAGPCDFDKDGDVITPINIWKFSGGKIETVKTMPAAGIPKI"
     gene            complement(5207..6415)
                     /locus_tag="VIR04_02705"
     CDS             complement(5207..6415)
                     /locus_tag="VIR04_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459692.1"
                     /translation="MDQVAILKAIFLIIPLTLLAFAVLRMRWLWRIPVGLAVGVIASF
                     AVVALFPGWLDYFVSFCIMASIYAILSLGLNSQWGYNGHLDFGIAGFFAVGAFTTALF
                     ITAPPSGLMVQYAQQLFGLHAPFLVGLIAAGVVSGVVGLLIALPVLRLRTDFLAIATL
                     GIAEIIRLIFQNERWLANGPQPLSGIPQPLSCVFDHPACSWLPSWIATAIAPLQPRNY
                     VYLYLVITVVTLAIIYLVLETAVRSPWGRALRAVRDEELSAAMNGKHVTSLRVQSFVL
                     GAVIMGIGGALYAPYMVTIDYSHFEPLFGTFIIWVMLMLGGSGNNKGAILGAFVIWGV
                     WSGTGFVTDALQSTLSTIAPEIARRTAFLRWMFVGLLLALIVLYRPEGILKEEKRVSD
                     FLKNVGPRLK"
     gene            complement(6431..>6648)
                     /locus_tag="VIR04_02710"
     CDS             complement(6431..>6648)
                     /locus_tag="VIR04_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459693.1"
                     /translation="DMGFTLLLPMFAAVILGSIGNTFGALVGAIVIGVAMQVTSSFMS
                     PAYGPAVAFALMVLALVVRPQGLFADK"
BASE COUNT         1606 a         1869 c         1841 g         1332 t
ORIGIN      
        1 ggcggctatc gccctgcatg ccaaaaaaac cggcggcctc atgacgcagg ccgatctggc
       61 cgcgcatcgc accgactggg tggaaccgat acgcatggct tatcgcgaca ttgaactggt
      121 ggaaattggc ccgaacggac aaggaatcgg cgcacttatg gcgctcggca tacttgatgc
      181 attcagcctg gcgggaaccg aagtggattc cgctgaaagt gtgcatctgc aaatcgaagc
      241 cacgaagctg gcgttcgccg atatgcatgc ctatgtcggc gacattgacg ccatgaccac
      301 agttcgcacc gaggatctgc tggatcgcgg ttacctgact gcccgcgcgc gcaacatcga
      361 tcccaggcgc gccacgtatc acgggccggg gaagccgcat catggaggca cggtgtatct
      421 gaccgcggct gatcgcaacg gcatgatggt ctcgttcata caatcctgct tcaaaggctt
      481 cggttcgggc gttgtcgtcc cggacaccgg aatcgcatta caaaaccgcg gctggggatt
      541 cagcacccgc gccggccacc ccaatcaggt ggggccaggt aaacgccctt ttcacacgat
      601 cattccaggt tttctcacgc gtcaggggca gccgctaatg acctttggcg ttatgggtgg
      661 ttcgattcag gcgcaaggcc atgttcaggt aaccagccgc attgcggact atggccagaa
      721 cccgcaggcc gccaatgatg ggccccgctg gcgcatcatg gacgataacc gcacggtaaa
      781 ggtggaatgg aacatgcccg aaaaaaccat acagggtctg cgcgaaaaag gtcacgacgt
      841 tcaggtttcc ccccgcttcg acctggaatt cggctgtgcg caaatggctc tcaagctgcc
      901 cgccggagga tattttgcgg catccgatca gcgcaaagat ggctatgccg tagggttctg
      961 actatactgc agtagtgcgg gacccgacaa aggagaagcc gccatgagca gcaattccgt
     1021 ctcggctcgc gactataaaa cgcaactgcg actgcttcaa atcgaactcg tcaagctgca
     1081 acgtcacatt atcagtgccg gcgacaaggt tctggtgctc ctggaaggcc gggatgccgc
     1141 aggcaaggac ggcagcatca agcgtattat tgcgcacctt agtccgcgcg aaacgcgtgt
     1201 ggttgcgctg ggaaaaccgt ccagccggga ccgcagcaca tggtatttcc agcgctacgt
     1261 ggcccacctg cccgccgccg aagagttcgt gctctttaac cgcagttggt ataaccgcgc
     1321 tggcgtcgag cacgtattgg gattttgttc ggatgacgaa tacgaagaat tcatgcagtc
     1381 ggcgccgaaa tttgaagaga tgctcgtcca ttcgggcatc aagttgatga agtactacct
     1441 tgacattaac aaagaagaac aaaagcgccg tctgctcgac cgtcaacgcg acccgctgac
     1501 gcaatggaaa atcagcccca tcgatgccgt ggctgtgaaa cactggaagg cctattccga
     1561 ggcacgcgac gaaatgctat tgcgaacgca caccaaagtc gcgccatggc gcatcgtgcg
     1621 agcggacgac aaacgccttg cccgcctgaa tctgatgcgc gatatcctga ctcgattacg
     1681 ctacgacggc aaaaaaaaca agctcgtgcg ccccgacaca gatgtggttt ttgagttcgc
     1741 acccgaatgc atcagcggca agcggctggc acgctgaaac cgtcacaatg gttcatccgc
     1801 gtcgcgggtg gttgaaactt gagcggttca agcgaataat ggaattgatg cagcggccca
     1861 gcaatttgtc tttgtcagga ggcacatcat gccagctaaa tcccaagctc aacaaaaggc
     1921 ggccggcgcg gcgctgtcgg caaaacgcgg agaaacgaag cttgccaagc ttaaaggttc
     1981 gtcgaagtcc atgtacaaat ccatgagcga gaaagaactc aaggaactgg catccacgcc
     2041 gcgcaaaggc aagccgaaac acaaatccga ttcatagcgg aaagcccgcg ccccgactct
     2101 acttcatcac ttttacttcg ggcaaggctt tgagttggtc tttcgtgtag ccttcaagga
     2161 ccagtttatt gttcctttct ttgatcttgt cgaaggggat ggcgacatcg tgtgcgccca
     2221 tgccaagaaa gccccccgcc ccgaccacta aagtggccgg tttcccatcg ggggtaagca
     2281 caacgtcgtc gatatcgccg attttcacgt ccttttcgtt aaagactgtc ttgcccatca
     2341 ctttgtgttt cacgctccaa ccgctaattg tctgcgcagg tggtgcggca ggggtcggca
     2401 ttgtcctgga gccgggcatg ccgcccggca taccgggtgc tccgggggcg gccggagtcc
     2461 cgggagcacc cggagcaggc gcggtcccgg gcgtggcagg ctgccccgtt tgcgcatagg
     2521 cggcgggggc aatgacgaat ggcagaaaac aagcgctgat aatgagaatt tttttcatga
     2581 cagtttccct tagaaaaatc ggtttagtga cttggactta aacgatccga cactggcggc
     2641 cagttccggt gctacggagc gcgtcccgat atgaggcgaa tcagcaccat gacgatggcg
     2701 atgacaatca agatatgaat gaaaccgccc agcgtgtagg acgtaaccag tcccagcagc
     2761 cataaaatca caagaatcac cgcaatggta tagagcatgt tgcttctcct ttttataaga
     2821 ttcgccgctt ttggcagtat gcgaagcgct acttagtcgc ttcgtggccg actacgcctc
     2881 cgacagcggc gccgcccaaa gtcccaagtg tgctgccgcc ggtcaatacg gcgccaccta
     2941 ccgcgcccac gcccgcgccc acagcagtgt ttctgccctg tctcgacatt ccgctacagg
     3001 cgctaaggct tagcagtacc gttgctgtca gtgcacatac agttgctcgt ttaatcgttt
     3061 tcataacgac accccttaat gttggttaag cgaaagtgtt catgtcaccc cagcaactct
     3121 catgcccggg tgcacaaaca ccttgctcgg catgaaactt gctgcattgt ttgttgggtc
     3181 agcttgtctg cattacttct cggggagctt tatcatgatt acgcaaaaac gcatgcttgt
     3241 ccctatgatg atggcggtgt ttgcgctgac cgcctgcgtc gcccagcctt acggggttgc
     3301 accacccgcc acatcttatt atcgcggcac cacaggcaat atgatcccgg cgaccaacga
     3361 tgcgtcgatt aatgcgaatg tggttgcggc cgtccaaagg tcgagcggag gcgttactaa
     3421 ccttcaggtg acgaccctca acggcgtcgt gacattacgc gggacggcgg acagccggtt
     3481 agtggcggag aacgacgttc aggcagcgcg tcaggtccct ggcgtacaac gggttgatta
     3541 cgatatccag atattgggcc gctagtacgg ctgagcgtcg tgcgttcggt caggcgtttt
     3601 ctttcccgtc tgtaaacgcg tggtcgcaac attgcccata actgcgcctg cgcatcggct
     3661 gaatatgtct tgacagacac tgccaatctg gctgtaatct gcaggcggac tgcgccaaac
     3721 tcgcaaagaa tcgagcccga tgcgccactt ggaagaaacg aaggtcaacc cctaaaggag
     3781 ataggttatg agtgatcaaa actcaagtcc catgagtcgt cgcaaattct ttataaacgt
     3841 gggcgccgtg ggaggtgcgg tcgttcttag cagcgcattt ccaaataaaa cattcgctgc
     3901 ggcgccgccg gccattaatt tgggccagat tgcgccaatg acgggagcgg cggcggaatt
     3961 tggtccctat tacagggacg cggtccagct tgccgtcgac cagatcaaca aggctgcgca
     4021 agaagtgttt ggaggcccga tcattgcaaa gcacgttact gtggatacaa caacggttcc
     4081 tacagttggg gtgcaggcgg cgcgtcaaat ggtggatacc gacaaggtgc cggcgatcgt
     4141 aggcggttgg tccagcggcg tcactgtcgc ggtcgccact tccgtaacca tcccttcggg
     4201 cgtattgcaa gtcgccaacg gggccacgtc gccattgatc accgtgctgc cggccgatgc
     4261 caagtcggat ctgctatttc gaactaccgc atcggatgct ctgcagggcg ttgtggctgc
     4321 gcagcttatt aatggtgaaa tcatcaagga ttacaagttc aaatccgtat cgaccatata
     4381 tatcaacaat ccttacggtc aggggttatc gaattcgttt gcgaaatcgt tcgaacatcg
     4441 cggcggcaag gtgtttgccc aggtgcctca ccccgaacag gttcagccca cttataaatc
     4501 ggaactggcc ttggcgctca agaacaagcc tgacctgttg ctggccgcca gttatccgtc
     4561 gcataccgcc gtgtttctca aagagtcacg cgacattttc aatttcacca gttgggaatt
     4621 gaccgacggc aatgcctcac tggacgtatt gacagccgta ggcgcaaaga acatggacgg
     4681 aaagatgggc accgcgccgg gtgaagacac cacgaccgca gcctataagg attttgccaa
     4741 gaactttttg gcagcctata aatatgatca cataccgccc tttaccgctt cgacgtacga
     4801 cgccgcgatg actatcggtc tggcgctggc caaagtaatt gccaacggcg aaaccaacgc
     4861 aagcaaaatt accggcggcg cactgcgcga tcagcttcgg gtcgtgtcca accctccggg
     4921 cgacgaaata gaaggtggcg accaggggcg tattacagag atgctcaaaa tgatcaaggc
     4981 gggtaaaaag atcaactaca cgggcgccgc aggtccgtgc gatttcgaca aggatggcga
     5041 cgtcatcacc ccgatcaata tatggaagtt tagcggcggc aagatcgaga cagtaaagac
     5101 gatgcccgct gcaggaattc caaaaatata ataggaagcc gcgcagcatt cgttgccgcg
     5161 atgtatgcgg cgctgttact tgaccccttt caagcgaaag gggtttttat ttcagcctgg
     5221 gtccgacatt tttcaggaaa tcggacactc tcttttcttc cttcaaaatc ccttccgggc
     5281 gatacagcac aatcagcgcc aatagcaggc ccacaaacat ccagcgtaaa aaagcggttc
     5341 gtctggctat ttcgggcgcg attgtgctta aggtggactg aagcgcgtcg gtgacaaatc
     5401 cggtgccgct ccagacgccc cagataacaa aagctccaag aatggcgccc ttgttgttgc
     5461 cactgcctcc cagcatgagc attacccaaa tgatgaaggt gccaaatagc ggttcgaagt
     5521 ggctgtaatc aatggtgacc atgtacggcg cataaagggc gccgccgatg cccattatta
     5581 ccgcgcccag tacaaatgac tgcaccctca acgaagtgac atgcttgcca ttcatggcgg
     5641 ctgataattc ttcgtcgcgc acggcgcgca acgcccttcc ccatggtgag cgcaccgcgg
     5701 tttccagcac aaggtaaata atcgccagcg tgacgactgt gataaccaga taaaggtaga
     5761 cataatttcg gggctgcagc ggcgcaattg ctgtggcgat ccagctgggc agccaactgc
     5821 atgcggggtg gtcgaacaca caggaaagcg gctgtggaat gccgcttaga ggctgaggcc
     5881 cattggcaag ccagcgttca ttctgaaaaa tgagccgaat aatttccgca atgcccaaag
     5941 tggcaatcgc aaggaagtcg gtccgcaatc gcaggaccgg caaggcaatt agcaaaccca
     6001 ccacccccga cacaacgcct gcggcgatca ggcccacaag aaatggcgcg tgcaaaccga
     6061 acaattgttg cgcatattgc accatgagcc cgctgggggg cgccgtaatg aaaagcgcgg
     6121 tggtgaaggc gcccacggcg aagaagcccg caattccgaa gtccagatgg ccgttatagc
     6181 cccattgcga gttcaggccg aggctgagaa tcgcgtagat cgaggccatg atgcagaacg
     6241 acacgaagta atcaagccaa ccgggaaaca acgccactac cgcgaacgag gcaatgacgc
     6301 caacggccag cccaaccgga atgcgccaca accagcgcat gcgcaacacg gcgaacgcaa
     6361 gtagcgtaag cgggataata agaaaaattg ccttgagaat ggcgacctga tccatagaat
     6421 cttccttctc ttatttgtcg gcgaaaagcc cctgggggcg caccaccagg gcaaggacca
     6481 ttaacgcaaa cgcaacggcg ggtccgtagg cgggactcat gaatgaagac gtcacctgca
     6541 ttgccactcc aataacgatt gcgccaacga gcgcgccgaa agtattgccg atgctgccca
     6601 ggatcacggc cgcgaacatc ggtaaaagca aggtaaaccc catatccg
//
LOCUS       DATCXU010000124         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_21233,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000124 DATCXU010000000
VERSION     DATCXU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21233"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="metX"
                     /locus_tag="VIR04_02715"
     CDS             complement(<1..307)
                     /gene="metX"
                     /locus_tag="VIR04_02715"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929590.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY9459694.1"
                     /translation="MAPFSTTFTTSPIVTPIPVQAPPAPDSPIGSVGLVTPQFIAFDE
                     PLPLLSGQVLPQYELAVETYGVLNAAHTNAILICHALNASHHVAGIAANDPSDIGWWD
                     "
     gene            530..784
                     /locus_tag="VIR04_02720"
     CDS             530..784
                     /locus_tag="VIR04_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9459695.1"
                     /translation="MKTVTKPLTTRELTCLRWAAIGKTSWEVGVILGLAERTINFHIH
                     NACKKLDVHGRQAAITIALQTGLLPALTEPPPPASWRTKQ"
     gene            937..2498
                     /gene="gshA"
                     /locus_tag="VIR04_02725"
                     /pseudo
     CDS             937..2498
                     /gene="gshA"
                     /locus_tag="VIR04_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            complement(2519..3793)
                     /locus_tag="VIR04_02730"
     CDS             complement(2519..3793)
                     /locus_tag="VIR04_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HEY9459696.1"
                     /translation="MDASRPNSLDRSFKGFPPMAAPCSVETVGQYKWDLFNDDLPYPI
                     AVIKQAELEHNLRWMQAFADERNVFIAPHGKTTMSPQLFRRQLELGAWGLTFATVYQI
                     GAGLEAGAKRIIIANQVLGCADLDALAYWLESMQGLRVWFLVDSQAQVDQIESWTHRR
                     ASTLRFDCLLEIGIEGQRTGCRTFDQAINVARRIRNSNSLSLGGVECYEGVLAHCQSE
                     RDRDAVEALMARVKDIATECDRLRLFEDDQIIISAGGSAVFDLVVPGLRASLSKPVTG
                     VLRSGCYITHDHDFYSKMLRNVEQRQGLKDSLQPALEVWTMVQSAPEPGLAILSCGKR
                     DISYDLSMPVVTHYCPRGSRSKLPAPADWVISALNDQHAYLHFAAESSGPMVGDRVIL
                     GISHPCTTFDKWSWLPIVDEAGVVTSAVTTRF"
     gene            complement(3771..4970)
                     /locus_tag="VIR04_02735"
     CDS             complement(3771..4970)
                     /locus_tag="VIR04_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY9459697.1"
                     /translation="MQTISPSSCLPGDLNDALLVGRLWRPGPIDGPSVIVVRGGQVFD
                     ITPAVRTTADLLEHPNAVDLARNAPGEPLGAVDALVHASLKRAEPDELRLLAPCDLQP
                     IKACGVTFAVSLIERMIEEQAGGDVGKAQELRQTINQHVGADFSKVRPGSGAALKLKA
                     ELERRGAWSQYMEVGIGPDAEVFSKAQAMSSVGLGAQIGLLPISKWNNPEPEVVLAVN
                     SRGEIMGASLGNDVNLRDVEGRSALLLGKAKDNNGSCSIGPFIRLFDERFTLDSVRNA
                     SVSLKIEGADDGFVLEGISHMSEINRDPADLVKQTCGAHHQYPDGFMLFLGTMFSPIK
                     DRDEKDGGFTHHLGDVVTISTPTLGSLVNTVQLSTEIAPWTFGVRALYRSLGERYGNQ
                     DGCKPAK"
BASE COUNT         1182 a         1479 c         1306 g         1016 t
ORIGIN      
        1 tgtcccacca gccaatatcg cttgggtcgt tcgctgcaat tcccgccaca tgatgcgacg
       61 cattcagcgc atggcaaatc aagatagcgt tggtatgcgc cgcattcaac actccgtacg
      121 tttcaactgc aagctcatac tgcggcaaca cctgtccgct aagcagcggc aaaggctcgt
      181 caaaggcaat gaactgcgga gttaccaggc cgacggaacc tatcggcgaa tccggcgcgg
      241 gcggcgcctg gacgggaatg ggcgtaacga tgggggacgt cgtaaatgtg gtggaaaatg
      301 gggccataaa gcatctttag ctggatttat caggcgcccg caagcggatc aggcaaatcg
      361 gcgccaaaac gaatactgat tctatcacta acttccgggg cggcatcctg gcccgtgcgc
      421 ccgaaatttt ccgtttatcc tcccctgatt ttaccttgtt gataaaactt gatacatttg
      481 acatacaaat gtaatgaaag cacatatctg acgaggggga aacactgtaa tgaagaccgt
      541 gacaaagccg ctgaccaccc gggaattaac gtgcctgcgc tgggcggcga ttggcaaaac
      601 aagctgggaa gtgggcgtca tcctgggtct tgcggagcgc acgatcaatt tccatattca
      661 taacgcctgc aaaaaacttg acgtccacgg acgacaggca gccatcacga tcgctttaca
      721 gacgggtttg cttcccgctc ttaccgagcc gccaccgccg gcatcctggc gcacaaaaca
      781 atgattgcgc cggaaaaaat cccatcggct ttaaacagct ctcccgcaca gtcagccggc
      841 aaggcaggac gcaaggccgg tgcgtctgtt taacgcccag ccgatgaagc taaggtaaaa
      901 tcgccagcaa atcagcctct ttaaagaacc tttgcgatga cttcaagcac caagcgccac
      961 aacctgctaa ctgaaggtgc cgatttattc aacggtatct tgcgcgggat cgaaaaagag
     1021 ggcttgcgag tcgataatct gggcgtattg gctaaaacgc cccaccccaa ggtcctgggt
     1081 cgcgcattga ccaaccctcg catcaccacc gactattccg aagctttgct ggagctgatc
     1141 acgggcacgc ataattccag cgcaggcgtt ctgtcggaac tggaggacac gcaccgtttc
     1201 gtggcatggg agttgccgga cgaactgatc tggaatcagt ccatgcccgc gcaactgcct
     1261 gccgatgccg acatacccat tgcgtggtac ggcacatcga ataccggaat gttgaaacat
     1321 gtttaccgcc gggggctggc cgaacgctac ggcaaaacca tgcaatgcat tgccggcgtg
     1381 cactataact tctccctgcc agacggcatt tgggacctgc tggaagttcc ggggtcctcg
     1441 gtcatgcaac agcggacgac cgggtacatg gccctgattc gcaattttgt gcgctatgca
     1501 tggctgctca tgtatctgtt tggcgcttct cccccgtatc aaagagcttt atgcacggcg
     1561 ccgatcaccc tctgtcgaca ttcgacgacg atacgctgta tctgccctac gcaaccagtc
     1621 tgcgcatgag cgatttgggc tatcagaaca aggctcagtc aaagttgcaa ctttgctaca
     1681 acgatctggc cacatttttg cagcgcactc gcgacgccgt caccacaccc tggccagctt
     1741 acgaggccat aggcacgcac cgcaatggcg aatggatcca gctcaacaca aatattctgc
     1801 agatagaaaa cgaatactac gcaagcattc ggcccaaacg caccacggga cgcggcgaac
     1861 ggccaactac ggcgctggcg gcgcgcggcg tgcaatacat tgaagtgcgc tgtctggata
     1921 tcgacccggt ttcggtgaca ggcatttcct tgcaaaccag ccacttcatt gatgcatttc
     1981 tgctcttttg cgccaccgaa gaaagcccct tcttccccaa gggcgggtat tgccaggaca
     2041 gtcacgacaa cttcgccctg acggccaaga cgggacgcaa accgggtttg atgctaaaaa
     2101 agcccgacgg ggaaatcggt ctgacacagt ggggcatgga gttgctcgat cgcattgagc
     2161 cctatgccga catactcgat acggcttacg caagccatgc acacggcgac tcggtcaagg
     2221 cgcagcgaat caaagtgcag gacagcgaac aaacgccttc ggcgcacttg ttgcaacagt
     2281 tacgcgaaca gcggctcagt tttcacgact attcgttaaa acaaagcaga caacaccgcg
     2341 acaccctgct ggccacgccc ctttcagccg cgacgcaaaa cgccttgaaa gaaatcgtgg
     2401 cgcaatcttt tgcggaacaa acacggcttg aacagagtga taccgaagat ttttcagcct
     2461 atgtcgcgcg ctacgaggcc gcgctgacgc aatcctgaca ttgctcacac tgtcgtttct
     2521 aaaagcgcgt ggtcactgcc gaagtcacca cgcctgcttc gtcgacaata ggtagccaac
     2581 tccatttatc gaaagtggtg caaggatgcg aaatccccaa gatgacccga tcgccgacca
     2641 tgggtccaga cgattcagcg gcaaaatgca aataagcgtg ctgatcatta agcgccgaaa
     2701 taacccaatc cgcaggtgcc ggcaatttcg accggctgcc ccgggggcag taatgtgtaa
     2761 cgacgggcat agacagatcg tacgagatat cacgcttgcc acaacttaaa atcgccaggc
     2821 caggctcggg cgccgactgc accatcgtcc agacctccag ggcgggttgc aagctgtctt
     2881 tcaatccctg acgctgctcg acgttgcgca gcatcttcga ataaaaatcg tgatcgtgcg
     2941 taatgtagca acccgagcgg agcacgcctg tcaccggttt ggacagcgat gctctcaacc
     3001 ctggaaccac cagatcaaaa acagcagacc ctcccgcgct aataatgatc tggtcatcct
     3061 cgaacaagcg caggcggtca cactcagtgg cgatatcttt aacgcgcgcc atcaatgctt
     3121 cgaccgcgtc acggtcgcgt tcgctttggc aatgagcaag aacgccttca taacactcga
     3181 caccacccaa gctcagactg ttggaattcc ttatgcgccg cgccacatta attgcctgat
     3241 caaacgttcg gcatccggtc cgttgtcctt caatgcctat ttccagcaag cagtcgaacc
     3301 gaagggtgct ggcgcgtcta tgcgtccagg actcgatttg atcaacctgc gcctgcgaat
     3361 cgaccaagaa ccacacgcgc aagccctgca tgctttcaag ccaataagcc agcgcgtcga
     3421 ggtcagcgca accgagcacc tgattggcaa taataatgcg tttcgcgccc gcttccaggc
     3481 cagcgccaat ctgatacacc gtggcaaagg tcaggcccca cgctcccaat tcaagttggc
     3541 ggcgaaacaa ttgcggcgac atggtggttt ttccgtgagg agcaataaaa acattccgtt
     3601 cgtcggcaaa cgcctgcatc cagcgcaagt tatgctccag ttcggcctgc ttgataaccg
     3661 cgattggata aggaagatcg tcgttgaaca aatcccattt atattggccg accgtttcga
     3721 cagaacacgg cgcggccatg ggcggaaacc ccttgaagct tcggtccagg ctatttggcc
     3781 ggcttgcatc catcttgatt cccatatcgt tcgccaaggc tgcggtacag tgcgcgcaca
     3841 ccgaaagtcc atggggcaat ttcggtcgag agctgcacgg tattgaccag gcttcccaga
     3901 gtgggggtgg aaatcgtcac cacatcgccc agatgatgcg taaagccgcc gtctttttca
     3961 tcgcgatcct tgattggcga gaacatcgta cccaggaaga gcatgaaacc gtctggatat
     4021 tggtgatggg cgccgcatgt ttgcttgacc aggtctgccg gatcacggtt gatttcgctc
     4081 atgtggctga tcccttcaag cacgaaacca tcgtcggcgc cttcgatttt cagcgaaacg
     4141 ctggcgttac gcaccgaatc cagcgtaaag cgttcgtcga acagacggat gaacgggcct
     4201 attgaacatg aaccattgtt gtctttggct ttgcccagca gcaaggcgct gcggccttcg
     4261 acatcgcgca agttgacatc gttgcccagg ctcgcgccca tgatctcgcc gcggctgttc
     4321 accgccagca caacttccgg ctcgggattg ttccattttg atatgggaag caaaccgatc
     4381 tgggcaccca gaccaaccga tgacatggcc tgcgccttgg aaaacacctc ggcatcgggg
     4441 ccgatcccca cttccatgta ttgcgaccat gcgccgcgcc gttcaagctc tgccttcagt
     4501 ttcagcgccg ccccggaacc aggacggacc ttggagaaat ccgcgcccac atgctgatta
     4561 atggtctggc gcaattcctg cgccttgccc acatcgccac ccgcctgctc ttcaatcatg
     4621 cgctcaatca ggcttactgc aaacgtgacg ccgcaggcct tgatcggttg cagatcgcaa
     4681 ggagcaagca gcctgagttc gtcgggttcg gcgcgcttca ggctggcatg caccagcgcg
     4741 tcgaccgcgc caagcggctc gccaggcgca ttgcgcgcaa ggtcaacggc attgggatgt
     4801 tccagcagat cggcggtcgt cctcacggcc ggcgttatat cgaacacctg gccgccacgc
     4861 accacaatca ctgatggacc gtcaattggt cctggtcgcc acaggcggcc gacgagtagc
     4921 gcgtcattca ggtcgccggg caagcaagag gaaggggaaa tagtttgcat gtcagattct
     4981 ttt
//
LOCUS       DATCXU010000125         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_974171,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000125 DATCXU010000000
VERSION     DATCXU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974171"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VIR04_02740"
     CDS             complement(<1..1447)
                     /locus_tag="VIR04_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEY9459698.1"
                     /translation="MANKKLSAVIEIGGAVAGSLKSALGTTKSKMLEIGGAVQQIEKQ
                     QKALTRTIEDQARKGKNANAVTVQYAQQELALLDKKVAKLRQVQTQFRTLQAAQDANL
                     SKRANLRGQLFDAVALGATLAAPMRAAVAFESAMADVRKVVDFDTAGEFKQMGDEVLR
                     LSTQLPMAADGIAQIVAAGGQAGIAKNELIQFATSATKMGVAFDITADEAGQMMAEWR
                     AAFKMSQPEVDSLADKINLLGNTTAASAPRISAIVRRVGPLGEVAGVASGEIAALGAT
                     MVGVGIQEEVASTGIQNMMLGLVVGTAATKKQQTAYDALGLSAEAVAKRMQVDARGAI
                     LSVLEAVKKLPKETQAATLSQLFGKESIKAIAPLLTVTENLVANLDKVADAQAFNGTV
                     NKEYAARAATTANNMQLFQNRVAVLGVTLGNILLPPLNATMATIGPILTTVADLATEF
                     PMVTQAIVMGGAALIGLKVAGIAAGYAWTFLR"
     gene            complement(1572..1865)
                     /locus_tag="VIR04_02745"
     CDS             complement(1572..1865)
                     /locus_tag="VIR04_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEY9459699.1"
                     /translation="MEKKELPEWLKENADGSVDITLAGKKRLTIDGVEVTALRMREPT
                     VDDQLAQEKIQGSAAEKEIGLMANLCQVTPAQISGLSLRNYKRVQAAFVGFTD"
     gene            complement(1944..2450)
                     /locus_tag="VIR04_02750"
     CDS             complement(1944..2450)
                     /locus_tag="VIR04_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HEY9459700.1"
                     /translation="MAARDVRKNINLFVDGRGYAGQVEEFNAPKLTLVTEEFRAGGMD
                     APVALTMGMEALACSFSLLAYDADVLALFGFAEGNEVPLVAREALESFDGTTKGVVHT
                     MRGKITSIDPGTSKPGEKASMAVEVSLVYYRLQHGELTVHEIDVENMVRIINGRDQLA
                     EQREALGI"
BASE COUNT          449 a          834 c          723 g          599 t
ORIGIN      
        1 cgcgcaggaa cgtccaggcg tagccggccg caatcccggc caccttcaaa ccaatcagcg
       61 cggcgccgcc cataacgatg gcctgcgtca ccatggggaa ttccgtcgcc aggtcggcca
      121 cggtggttag aatcgggccg attgttgcca tggtcgcgtt cagcggcggc agcagaatat
      181 tacccagcgt cacgcccagg accgccacgc ggttctggaa tagctgcatg ttgttcgcgg
      241 tcgtggccgc gcgcgcggca tattccttgt tcactgtgcc attgaatgcc tgcgcgtcgg
      301 ccactttgtc caggtttgcc accaggtttt ccgtgactgt caacagcggc gcgatggcct
      361 tgattgattc cttaccgaac agttgcgata gcgtggccgc ctgggtttcc ttcggtagct
      421 ttttgaccgc ttccagcaca gacaggatgg cgccacgcgc gtccacttgc atgcgcttgg
      481 ccacggcctc ggcgctcagt cccagcgcgt cataggcggt ttgctgtttc ttcgttgcgg
      541 cagtgcccac cacaaggccc agcatcatgt tttgaatgcc ggtcgatgcc acttcctcct
      601 ggatacctac gcccaccatc gtggcgccaa gggctgcgat ttcaccggat gccacgccgg
      661 ccacttcgcc cagcgggcca actcggcgca cgatggcgga aatgcgcggc gcgctcgcgg
      721 ccgtggtgtt gcccagcaag ttgattttgt cggccaggct gtccacttcg ggctggctca
      781 ttttgaaagc cgcgcgccac tcggccatca tctggcctgc ttcgtcggcg gtgatatcaa
      841 aagcaacgcc catttttgtg gcgctggtgg cgaattgaat caactcattt tttgcaatgc
      901 ccgcctggcc gccggctgca acaatctgcg cgatgccatc ggcggccatc ggcaattgag
      961 tggacaggcg cagcacttcg tcgcccatct gtttgaactc gccggcggtg tcaaaatcca
     1021 ccaccttgcg aacgtccgcc atggccgatt caaacgcaac ggccgcacgc atcggcgccg
     1081 caagcgttgc gcccagcgcc accgcgtcaa acagttgtcc gcgcaggttc gcgcgtttgg
     1141 aaaggttcgc gtcctgcgcg gcctgcagcg tgcggaattg cgtctgcacc tggcgcagct
     1201 tggccacttt cttatccagc agcgccagct cctgctgcgc atattgcacg gtcactgcat
     1261 tggcgttttt gcccttgcgc gcctggtcct caatcgtgcg cgtcaacgcc ttttgctgct
     1321 tctcgatctg ctggacggcg ccgccgattt ccagcatttt ggatttggtg gtgccaagcg
     1381 cgcttttcag cgaacccgca acggcgccgc caatctcaat aactgctgaa agttttttgt
     1441 tcgccattac tttttcggca gtccatcaag ccaccagaca aaacgagaaa caggcatggc
     1501 cagaatttcc tgctcccccc atcctgtatg gctggccaag gccagcgcgc cgtcccggat
     1561 ataaccggcg cttagtcggt aaaacccacg aaagcggcct gtactcgttt gtagtttcgc
     1621 agcgacaggc cgctaatctg cgccggcgta acctggcaca gattcgccat caatccgatt
     1681 tccttttcag cggcgctgcc ttggattttc tcttgcgcaa gctggtcgtc tacggtcggt
     1741 tcgcgcatgc gcagggccgt cacttcgacg ccgtcaatcg tcagccgctt tttgccggcc
     1801 agcgtaatgt caacggaacc gtctgcgttt tccttcagcc attccggcag ttctttcttt
     1861 tccatttgct ttgcccgttc gtgatttgtt tttaaatcgg ccggcgcaac tggccggccg
     1921 tacactgttc ttttatccag tgattaaata cccagtgctt cccgctgctc ggccaactgg
     1981 tcccggccat tgataatgcg aaccatgttt tccacgtcga tttcgtgcac cgtcagttca
     2041 ccgtgctgca gccggtaata aaccagcgag acttccacgg ccatgctggc tttttcgcca
     2101 ggtttgctgg tgcctgggtc gatgctggta atcttgccgc gcatggtatg aaccacgccc
     2161 ttggtggtgc cgtcgaaact ttccagggct tcgcgcgcca ccagcggcac ttcgttgccc
     2221 tctgcgaatc cgaacagcgc cagcacgtca gcgtcatagg ccagcaggga aaagctgcag
     2281 gccagcgcct ccatgcccat ggtgagcgcc accggcgcgt ccatgccgcc agcgcggaat
     2341 tcttccgtaa ccagcgtcag cttaggcgcg ttgaattcct ccacctggcc ggcgtagccc
     2401 cggccgtcca caaacaggtt gatatttttc cgaacgtcgc gcgcggccat cttagaaaat
     2461 ctccgttagg tagtcctgca ccatgtggct gcggaacgtg atgtgttccg ccgggtaagg
     2521 cggtgtgaaa tcgaaatcaa aatagacttt gccctgggcg atctggtccg gtgaattcaa
     2581 cgccggatcg gcccagcagc ggccg
//
LOCUS       DATCXU010000126         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_650314,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000126 DATCXU010000000
VERSION     DATCXU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650314"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VIR04_02755"
     CDS             <1..686
                     /locus_tag="VIR04_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY9459701.1"
                     /translation="QITEASPYLDALDKLLTLIETLAKDGIHIRHLDLGGGIGIRYTN
                     ETLLPPKVLLDSVFERLQAHGLAHLQIILEPGRSLVGNAGVLLTRVQYLKYSDAKNFA
                     IVDAAMNDLIRPTLYNAWHSIEAVAPRNNDHAAPVATYDIVGPICESGDWLARDRELA
                     IRQGDLLAIMSAGAYAFAMASQYNTRPRAAEVIVDGGQVHLVRPRETVESLFADEAVM
                     PPVVAALQR"
     gene            complement(731..2089)
                     /gene="ccsB"
                     /locus_tag="VIR04_02760"
     CDS             complement(731..2089)
                     /gene="ccsB"
                     /locus_tag="VIR04_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY9459702.1"
                     /translation="MSDTIIQPRMPMWQEAMTATGDSRGHRGRPDWTDIVFFLALASG
                     AAYVLSTYGGSMDYYEKMILVGAVAAFSWLAWLWRPVRTLMIASGLTALFAIWLYSNG
                     GGLGQGDLSRAESVFFLKYLFSSQSAILWMCSLFVLAAVCYWIGIFSKTAAWLGTVLT
                     WGAVYAGLTGLLVRWREGHLMGADIGHVPVSNLYEVFVLFSLITALFYLYYERRYATR
                     ALGGFVLLVISSAVIFLLWYSFTRDAFQIQPLVPALKSWWMKLHVPANFIGYGTFSLA
                     AMVGFAYLVKEHGQTVSRTRLLPIFILGAVLCAEPMVFGTKELSPIWMLYFGIGAIVV
                     GGILALRKPIADKLPSLDILDDIMYRAIAVGFAFFTVATILGALWAADAWGTYWQWDP
                     KETWALIVWLNYAAWLHMRLMKGLRGTVAAYWALGGLLVTSFAFLGVNMFLSGLHSYG
                     EL"
     gene            complement(2101..>2895)
                     /locus_tag="VIR04_02765"
     CDS             complement(2101..>2895)
                     /locus_tag="VIR04_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY9459703.1"
                     /translation="DGQSHQYNNYMLPIKLDDNMVFLAGVRESPSQSYRYLRIPADHN
                     NSVVEFMNLRAALDDPAMRAEAADRFAAKNANTALQRPLLQKAAQGALETFAVKGFNG
                     IIDKVPEQDREKVLNFAVPMVQLSLAELRDVDRERLGEPPIDHSGPAEQSAEKWIRLA
                     LLAMANLPDYPSPVFMSLKHFDHVQASVFQVARSPGKNMVYLGCIFLVLGVFSMFYIR
                     ERRIWVWLKPQGQGTEMLAAMTSQRRTLDFNHEFERFKETFHRLST"
BASE COUNT          736 a          859 c          770 g          530 t
ORIGIN      
        1 cgcagattac cgaagccagc ccctaccttg atgcgctcga caaactgttg acgcttatcg
       61 agacgctggc caaggatggc atccacatcc ggcacctgga cttgggcgga gggataggca
      121 tacgttacac gaatgaaacg ctattgccgc ccaaggtgtt gctcgacagc gtgttcgaac
      181 ggctgcaggc gcatggactt gcccatctgc aaattattct ggaacccgga cggtcgctgg
      241 ttggcaatgc gggcgtactg ctgaccaggg tgcaatacct gaaatacagc gacgccaaaa
      301 actttgcgat tgtcgacgcc gccatgaacg acctgattcg acctacttta tacaacgcat
      361 ggcacagcat tgaagcagtt gcgccacgca acaacgacca cgcagcgccc gttgccacct
      421 atgatatcgt cggacccatt tgcgaaagtg gagactggct ggcgcgcgac agagagctcg
      481 cgataaggca gggggacttg ctggccatta tgtcggccgg ggcctatgcc tttgccatgg
      541 caagtcaata caatacccgg ccgcgcgcgg ctgaagtgat tgtcgacgga ggccaagtcc
      601 atttggtgcg cccgcgcgaa acagtggaaa gcctgttcgc ggacgaggca gtgatgccac
      661 ccgttgtcgc cgcgctgcag cgctgaaaca catcagcgca gcattgcaat tcttttagtt
      721 cccgcagctc ttacagttcg ccgtaggaat gcaggcccga caggaacatg ttcacaccca
      781 ggaacgcgaa gctggtgacc agcaggccgc ccaaggccca ataggcagcc accgtgccgc
      841 gcaggccttt catcaagcgc atgtgtaacc aggcggcgta attgagccag acaatcagcg
      901 cccaggtttc cttgggatcc cactgccaat aggtgcccca ggcatcggcg gcccataagg
      961 cgcccaaaat ggtggcgaca gtgaagaacg caaagccaac cgcgatcgcg cgatacatga
     1021 tgtcgtcaag aatatccagc gacggcagct tgtcggcgat aggcttgcgc aatgccagaa
     1081 ttccgcccac gacaatcgcg cctatcccga aataaagcat ccatatcggc gacagctctt
     1141 ttgtgccaaa caccatgggt tccgcgcaca aaaccgcacc cagtataaaa atgggcaaca
     1201 gcctggttcg agacacggtc tggccatgct ctttcaccag ataggcaaac ccgaccatag
     1261 cggccaacga gaaggtgccg taaccgatga aattggccgg aacgtgcagc ttcatccacc
     1321 aacttttaag cgctggcaca agcggctgaa tctggaaagc gtcgcgcgtg aatgaatacc
     1381 acagcagaaa aataaccgcg gaactgatca ccagcaatac aaaaccgccc aacgcgcgtg
     1441 ttgcgtaacg ccgctcgtaa taaagataaa acaaggcggt gatcagggaa aacaacacaa
     1501 acacttcgta cagattgctg acggggacat gaccaatgtc ggcgcccatc agatggcctt
     1561 cacgccaacg caccagcaac ccggtcaatc cggcgtatac agcgccccat gtcagcaccg
     1621 tgccaagcca ggccgccgtt ttgctgaaaa tgccgatcca gtaacagacg gcagccagca
     1681 caaagagcga gcacatccac aagatggctg actgcgatga gaataaatat ttcagaaaga
     1741 aaacgctttc cgcgcgcgaa aggtcgccct ggcccaagcc gccgccattg ctatagagcc
     1801 aaattgcaaa cagcgctgtc aaaccgctgg cgatcatcag ggttcgcaca ggacgccaca
     1861 accaggccag ccagctaaac gccgctacgg cgcccaccag aatcatcttc tcgtaataat
     1921 ccatgctgcc gccataggtg gacagcacat aggcggcgcc ggaggccagc gccagaaaga
     1981 aaacaatgtc agtccagtcg ggccttccac gatggccgcg cgagtcgccg gtggcggtca
     2041 ttgcttcttg ccacatgggc atgcgcggtt gaataattgt gtcagacatg gaacacctcg
     2101 ttatgtagac agacgatgaa acgtctcttt aaagcgctcg aactcgtgat tgaaatccag
     2161 cgtgcgccgc tgcgacgtca tggcggccag catctcggtg ccctggcctt ggggcttgag
     2221 ccaaacccaa atgcgtcgtt cgcgaatgta aaacatcgag aacacgccca ataccaagaa
     2281 tatacagcct aaataaacca tattcttgcc cgggctgcgc gccacctgaa aaacgctggc
     2341 ttgcacgtgg tcaaaatgtt taagcgacat gaaaacaggc gatggatagt caggcaggtt
     2401 ggccatggcc agcagcgcca gccgaatcca cttttccgcc gactgttccg ccggccccga
     2461 gtgatcgata ggcggctcgc ccagtctctc gcggtcaacg tcgcgcaact ccgccagcga
     2521 caattgaacc atgggcaccg caaaattcaa aactttttcc cggtcctgtt cgggcacttt
     2581 atcaatgatg ccattgaagc cctttacggc aaatgtttcg agcgcaccct gagccgcctt
     2641 ttgcaacaag ggccgctgca acgccgtatt ggcgttttta gcggcgaacc gatccgccgc
     2701 ctcggcgcgc atcgccgggt catcaagggc ggcacgcaag ttcatgaact cgaccaccga
     2761 attattatga tcggcgggga tgcgcaaata acgatagctc tggctcggtg attcgcgtac
     2821 gccggccaaa aacaccatgt tgtcgtcaag ctttataggc aacatgtaat tattgtactg
     2881 atgcgactga ccgtc
//
LOCUS       DATCXU010000127        12313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1039897,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000127 DATCXU010000000
VERSION     DATCXU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039897"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VIR04_02770"
     CDS             <1..648
                     /locus_tag="VIR04_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY9459704.1"
                     /translation="TDPIAACGYSEDEIRAVVDEARARHTYVMAHSYTAEAIERAVRL
                     GVRTIEHANLIDERVAQFMVEKGAYVVPTLVTYEALASEGAKYGLPPESVAKIETVRA
                     AGLATLEIFKRVGLKMGFGSDLLGPSHRLQSDEFRIRTQVLSPFEVIQSATLIGAEVL
                     NMQGRLGRLTVGAIADVLVVDGDPYKDINCLLGQGDHIPLVMKAGEIYYDELTGN"
     gene            655..4476
                     /gene="putA"
                     /locus_tag="VIR04_02775"
     CDS             655..4476
                     /gene="putA"
                     /locus_tag="VIR04_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY9459705.1"
                     /translation="MAYTTLGVKVDDAVRERLRSAAAVLGCTPHWLHKQALLSYIGKI
                     ERGQLPLEIAHLSEDDGVDLEPEEVATAVAPPFFEFGRDVQPQSVLRAAITSAYRRAE
                     QECLPLLLDQARMPDQDKIHALAVRLVESLRGKRTGGGVEGIIQEFSLSSQEGVALMC
                     LAEALLRIPDRATRDALIRDKISKSDWKSHMGGSSSLFVNAATWGLMLTGKLVAVNSE
                     QTLSKALTRLIGKGGEPLIRKGVNIAMRMMGEQFVSGQSISEALANNHKMEVKGFRHS
                     YDMLGEAAATAEDADRYYASYEQAIHAIGKASGGRGIFEGPGISIKLSALHPRYSRLQ
                     RERVMVELLPRLRALTRLARHYDIGMNIDAEEADRLEISLDLLDALCFDPDLEGWNGI
                     GFVIQAYQKRAPFVIDYVVDLAKRSRHRLMVRLVKGAYWDTEIKRAQVDGLEGYPVYT
                     RKVYTDISYLACARKLLASPEAIFPQFATHNAYTMAAIYHLAGQNYYQGQYEFQCLHG
                     MGEDLYEEVVGPVSEGKMNRPCRIYSPVGTHETLLPYLVRRLLENGANTSFVNLVGDE
                     SVPVEQLASDPVDVASRISPVGAPHEKIPLPRELYANAPSGARANSSGMDLTNEHRLG
                     SLSAALLATSGKAWRAEPMLGDTSLEWDGARAVPVLSPADHRDVVGQVIEADQADVAT
                     AIGYAVNAAPIWQSTPVAERARCLRRAAQMLEERTQALLGLIVREAGKSLSDAIAEVR
                     EAVDFLRYYAQQIEREFDNDAYRPLGAILCISPWNFPLAIFMGQISAALAAGNVVLAK
                     PAEQTALIAAQGIEVLRAAGVPPGAIQLLPGRGEVVGAALVACPQVRGVMFTGSTDVA
                     RIIARTLADRLDDQGRTIPLIAETGGQNAMVVDSSALTEQVVSDVLSSAFGAAGQRCS
                     ALRVLCIQEDNAEQVLGTLRGAMRELNVGNPDHLSVDVGPIIDAQAKLAIDQYVDGMR
                     AAGHRVDQIELSSDAHYGSFVAPTLVEIDDVATLKREVFGPVLHVLRYRRDELDTLLS
                     DINSTGYGLTFGVHTRIDETIKHVTSRVKAGNIYVNRNIIGAVVGVQPFGGMGLSGTG
                     PKAGGPLYLYRLLSVQPAALPPWADLSDGLPLTRALAGPTGESNTYQLLPRGVVLCVA
                     ETVEGAQAQWSAVVETGNRALFVDTQATKTFLATLDGVQRQSVQTVSDADIDQAEFDA
                     VLFEGDSDALRELNRRVASRDGPLVSVQGLTSDALSAGALYMRDRLLSERSLSVNTAA
                     AGGNAHLMSIG"
     gene            4500..5462
                     /locus_tag="VIR04_02780"
     CDS             4500..5462
                     /locus_tag="VIR04_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9459706.1"
                     /translation="MNVRQLEAFCKVMEVGSMSEAARLLGISQPAVSKSIRLLEQALK
                     LRLFKRSGDRLYPSMEAQRLYPSARRIFDEIQTTFELGKKLQAAEVGTLKIAATYSVT
                     AAYVTEAIEAFHNRRPLIDIQFMALPPRQIIDLVRTCEIDAGLLYEPISVPKVQTIPL
                     CDIEMICALPLLHHLGNKDVIDSRDLTNETVISFSGEAYGGDLLKRQCEAAGASWNVA
                     IAVNQTVVAMNMAAAGIGTAVIDSLALSDSVSGKVIIKPFRPLTVLHVSAIVPSERPL
                     SQLCKEFIDILANVVKRHSEQSSGFHKVAASPITFSAQARLQKS"
     gene            5522..6688
                     /locus_tag="VIR04_02785"
     CDS             5522..6688
                     /locus_tag="VIR04_02785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9459707.1"
                     /translation="MDFSMVEAHPEVREAIRALCAPFDDQYWRKKDKEGTFPEDFYRA
                     IAEGGWLGIAAPESYGGSNLGMTGALAMLQTIAESGAGMNGGTSVKINIFGLNPVIKF
                     GSDEQKERMIPAIISGKEKTCFAITEPDVGLNTTQLKTRAVRDGDHYVVTGAKTWIST
                     AQVADRMLLLARTTPLDQVEKPTDGLSLFLTPLDRNYVEVREIQRMGRCAVDANQMFF
                     DSLPVPAADRIGEEGQGFRYVFDSMNPERMVVAAEAVGIGRAALRKAARYANERVVFG
                     RPIGQNQGVQHPLARCWMDLEAANLMASQAAWLYDRGEHCGLQANSAKYLAGEAGFKA
                     CEAAMLTMGGNGFAKEFDVERYLRDIMVARIAPITAQLIFSYVAEKALGLPKSY"
     gene            6758..7588
                     /gene="paaG"
                     /locus_tag="VIR04_02790"
     CDS             6758..7588
                     /gene="paaG"
                     /locus_tag="VIR04_02790"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEY9459708.1"
                     /translation="MDRSSAPVSPASSASDDVVLYSLSEGLAVITLNRPDKLNSLNAA
                     MHSRLRGLLDEISAEPSVRAVLLTGSGRGFCAGQDLGERKREPGEPAPDLGRTIEKNW
                     NPLARALRYLRVPIICAVNGVAAGAGANIALACDVVIAARSASFVLSFSRIGLVPDAG
                     GTYYLPRLVGEARAMGMALFGEKVSAADAAGWGLIWRCVEDDELAEQARKLAMSLAQG
                     PTLGLGSLKQIMRASLDHSFEEQLELERRTQQRCGASEDYQEGVAAFMEKRAPQFKGR
                     "
     gene            7599..9287
                     /locus_tag="VIR04_02795"
     CDS             7599..9287
                     /locus_tag="VIR04_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9459709.1"
                     /translation="MSPANMFSATAGFGADGSPSVPDREPIQVLDLYPPHGDTLWGLV
                     KSRAAILGDRPFIFFNGVQLSWAAFRDKVVRVARLLRAHGVEPGDRVGIMADNSDDYV
                     VIFFATAYVGAIFIPINPRLTVAETAYILNNAQPALVFCVPASDAILRAACQSLEPLP
                     HIASLANSWEGLAAAGSAGDDAVNSGVDSVESAAPEDTALILYTSGTTGFPKGVMHSQ
                     RSIVMAGEYFVERLHLQPEDRLLCVLPFFHINALIYSLLGAVAAGSSLVIAPRFSASE
                     FWTLAAESGATQVNIIATVGRILMRRPRSEFVAGHHIRKLYGAPITADIYSAFQTEFR
                     IPVLIEGYGLTEAPGICSNPFNGPHKVGSIGIPTHHPDPGRPYAQMRVADESGRELPR
                     GSTGELLVNSPIAMQGYYRDTAASDAAFLNGWFRTGDLVRQEEDGYYTFIERQKDIIR
                     RRGENISGAELDRIIGQHGEVQTVAAIAVGSELGEDDILVAVIVKQGSTLTERELRKW
                     CEQRLNSAKLPRYIAFVDSLPYTPSHRIAKFQLRRDPDLRQRAVEFPDMPVVRV"
     gene            9349..10344
                     /locus_tag="VIR04_02800"
     CDS             9349..10344
                     /locus_tag="VIR04_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459710.1"
                     /translation="MKIRNVVAVLAGLGITMLGLSATASAAAGGAGAFPTHSVRMLVG
                     YPPGGNVDIAARIIAAELGKKLGQSVVVDNRAGASGQIAAAATAQAAPDGYTILLGAS
                     PELAIVTALKKKVPYDPLKDLQPISLATQVPFVLVVNPSVPAKTVKELIAYAKANPGK
                     LNYASFGNGSSNHLFAELFKSRAGVKIEHIPYKGGGPAMTGLLGGQVQMEFESLGVVL
                     PHVEAGKLRALAVAMPARSSLAPAIPTMAEAGLPGLEGGTWNGFVGPAGMSPAITAKL
                     SEKINEAMREPAVRKQLIEKGLIPSADSPKEFGAFIASEIKRWSRVAEAANIHID"
     gene            complement(10373..11380)
                     /locus_tag="VIR04_02805"
     CDS             complement(10373..11380)
                     /locus_tag="VIR04_02805"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY9459711.1"
                     /translation="MSEKPATRKLVIDGLVYFCDGDPAPFRAGGVTAANITVTHMHWG
                     LEETFTAFGQWHKRLAAPDSGWRLVRTAGDILAAQAQGRTGLIMGWQNSLPFGTELDR
                     VTAFHALGLRVVQLTYNEANEVGDGCLEERGGGLTRFGKTLVAEMNRVGIAIDLSHCG
                     ERTTIEAADASCKPVLVTHANAKGLDDQIRNKSDKAIRAVAKTQGIIGTSIHGFLNWD
                     NNPKHPPSLANFVRHAQYVADLVGVEHVGIGTDFSVAQSEESVRSILELSKGAYPETG
                     GKYAEAFGNSSAGRYPPETPTPAQFPHIIDALERGGFSSDEVAAIAGGNFLRVLGQIW
                     G"
     gene            11554..>12313
                     /locus_tag="VIR04_02810"
     CDS             11554..>12313
                     /locus_tag="VIR04_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9459712.1"
                     /translation="MSSGSGTVAVVGAGIVGVCIAYELRKRGADVLLIDRAEPGRGCS
                     FGNSGAISASSIAPLAMPGILASVPRMLCDANGPLYLPWRYLPKALPWLARFVWSARP
                     GPVNRAVADLAALHEGAVNRHVAMAREVGVPELILQRGHLHLYPDESALAKDAAGWDL
                     RRKYGFQFERLERDGIIALEPDVSPRYRVGMFLADHATVRNPFRYVQAIFHKYLEIGG
                     RALHDEAQALAPENNGEWRIGLSAGSQTVEKVVVA"
BASE COUNT         2459 a         3352 c         3800 g         2702 t
ORIGIN      
        1 accgatccga tcgcggcgtg tggctattcc gaagacgaga ttcgtgcggt tgtggacgag
       61 gcgcgtgccc gtcacacata cgtcatggcg cattcatata cggcggaagc catcgagcgc
      121 gccgtgaggt tgggcgtgcg cactattgag catgccaatc tgatcgacga gagggtggcc
      181 caattcatgg ttgagaaggg cgcttatgtt gttcccacgc tggtcacgta cgaggcgctg
      241 gccagcgaag gcgccaagta tggcctgccc cctgaaagcg ttgcaaaaat cgaaacggtg
      301 cgcgcagcgg gtctggcgac actcgagatc ttcaagcgag ttggcttgaa aatgggcttc
      361 ggctccgact tgcttgggcc ctcgcaccgt ttgcaaagcg acgagttccg tattcgcacg
      421 caagtgctgt cgccgtttga agtcattcag agcgcgacgc ttatcggcgc cgaggtgctg
      481 aatatgcagg gccggctggg ccggttgacg gtcggcgcta tcgcggatgt acttgttgtg
      541 gatggcgacc cctacaagga tatcaactgc ctgttggggc agggtgatca tatcccgctg
      601 gtgatgaaag ccggtgaaat ttattatgac gaactgacag gcaattgacg ttttatggcc
      661 tataccactt tgggtgtcaa agtagacgac gcagtgcgcg aacgtctgag gtcggctgcg
      721 gccgtgctgg ggtgcacgcc gcattggctg cataagcagg cattgctgtc atatattggc
      781 aagatcgagc gcggccagtt gccgctggag atcgcacatc tgtcggaaga cgacggcgtg
      841 gacctggagc ccgaggaggt tgcgacagcc gttgcgccgc cgtttttcga gttcgggcgc
      901 gatgtccagc cgcagtcagt gcttcgcgcc gccatcacgt cggcctatcg gcgcgccgag
      961 caggaatgtt tgccgctttt gctggaccag gctcgcatgc ccgaccagga caagattcac
     1021 gctttggcgg tcaggctggt ggaatcgctg cgtggcaagc gcacgggcgg aggggtagaa
     1081 ggcattatcc aggagttttc cctttccagc caggaaggcg tggcgcttat gtgcctggcg
     1141 gaagcgctgt tgcggatacc ggatcgcgcg acgcgcgacg cgttgattcg cgacaagatt
     1201 tcgaagagcg actggaagtc tcatatgggc gggtcgtcat ctctgttcgt caatgccgcc
     1261 acgtggggct tgatgcttac aggcaagctg gttgccgtca atagcgaaca gaccttgtca
     1321 aaggcgctca cacgcctgat tggcaaaggc ggcgaaccat tgatccgcaa gggtgtgaat
     1381 atcgccatgc gaatgatggg cgagcagttc gtatcgggcc agagcatttc tgaagcgctt
     1441 gccaataatc acaagatgga ggtcaagggc tttcgtcatt catacgatat gctgggtgaa
     1501 gcggcggcga cggccgagga cgccgaccgt tattatgctt cgtacgagca ggcgatccat
     1561 gccatcggca aggcgagcgg tggccgtggc atattcgaag gccctggcat ttcgatcaag
     1621 ctatcggcgc tgcatccgcg ttattcccgc ttgcagcgcg aacgggtcat ggtcgaattg
     1681 ttgcctcgcc tgcgagccct gacaaggctg gcgcgccatt acgacatcgg catgaatatc
     1741 gatgccgaag aagccgaccg cctggaaatc tcgctggatc tgctggatgc cttgtgtttt
     1801 gaccctgatc tcgaaggctg gaacggtatc ggttttgtga ttcaggctta tcagaaacgc
     1861 gcgccgttcg ttattgatta tgttgtcgat ctggccaaac gcagccgtca tcgactgatg
     1921 gtgcgtctgg tcaaaggcgc ctactgggat accgaaatca aacgcgccca ggtcgatgga
     1981 cttgaggggt atccggtcta cacgcgcaag gtctatacgg atatttccta tctggcctgc
     2041 gcacgcaaac tgctggcttc gcccgaggcc atcttcccgc agttcgccac ccacaatgcc
     2101 tacaccatgg cggcaatcta ccatctggcg ggccagaact attatcaggg gcagtacgaa
     2161 tttcaatgcc tgcacggcat gggcgaggat ctgtacgaag aggtggtcgg gcctgtttca
     2221 gaaggcaaga tgaaccggcc gtgccgtatt tattcgccgg tgggcacgca cgaaacattg
     2281 ctgccttatc tggtgcgccg tttgcttgaa aatggcgcca acacctcgtt cgtcaatttg
     2341 gtcggcgatg aaagcgtgcc ggtcgagcag ttggcatccg accctgttga tgtcgcaagc
     2401 cgcatttccc cggtaggcgc gccgcacgag aaaatcccgc tgccgcgtga actatacgcg
     2461 aatgccccgt cgggcgcgcg ggcgaactcg agcggtatgg atttaaccaa cgagcatcgt
     2521 ctcgggtcgt tgtccgccgc tttgcttgcc accagcggaa aggcatggcg agccgaaccg
     2581 atgcttggcg atacctcgct tgaatgggat ggcgcgcggg ccgtaccggt gctcagcccg
     2641 gcggaccatc gcgatgtggt gggtcaagtg atcgaggccg atcaggctga cgtggccacc
     2701 gccatcgggt acgctgtcaa cgccgcgccc atctggcagt ccacgcctgt tgctgagcgg
     2761 gcgcgatgcc tgcgccgcgc ggcgcaaatg cttgaggagc ggacacaggc cttgctgggg
     2821 ctgattgtgc gcgaagcggg aaaatcattg tccgacgcga ttgcggaagt gcgcgaggcc
     2881 gtcgactttc tgcgctatta cgcacaacag attgaacgcg agttcgacaa cgatgcctat
     2941 cggccgttgg gggccattct ttgcattagt ccatggaatt ttccgctcgc catcttcatg
     3001 gggcagatat cggccgcatt ggcggcgggc aatgtcgttt tggccaagcc agccgagcag
     3061 acggcgttga ttgcggctca ggggatagag gttttgcggg ccgccggggt gccgccgggc
     3121 gcgatacagt tattgccagg gcgcggtgaa gtcgtggggg cggcgctggt tgcctgcccg
     3181 caagtgcgcg gcgtcatgtt cacgggttcg actgacgttg cgcgcattat cgcccgtacc
     3241 ttggccgacc gcctggatga ccagggccgc accattccgc tgatcgccga gaccggcggt
     3301 cagaatgcca tggtggttga ttcgtcggcg cttacagagc aggtggtgtc cgatgttctg
     3361 agctctgctt tcggcgctgc cggccagcgc tgttcggccc tgcgcgtgct ttgcattcag
     3421 gaagacaatg ccgagcaggt gctgggtaca ttgcgcggcg ccatgcgcga acttaacgtc
     3481 ggcaatcccg atcatttgtc agtggatgtg gggccgatca tcgatgccca ggccaaattg
     3541 gcgattgatc agtatgtgga tggcatgcgg gccgctggtc accgcgtcga tcaaatcgaa
     3601 ttgtcatccg atgcgcatta cggctccttt gtagctccga cactcgtcga gatcgacgat
     3661 gtcgcgaccc tgaagcgtga agtattcggg ccggtgctgc atgtgctgcg gtatcgccgc
     3721 gacgagcttg atacattgct ttccgatata aacagcaccg gctatggctt gaccttcggc
     3781 gtgcacacgc gtatcgatga aacgatcaaa catgtgacct cgcgcgtgaa ggcgggcaat
     3841 atttatgtga atcgcaatat tatcggcgcg gtcgtgggcg tacaaccttt cggcgggatg
     3901 ggtttgtcgg gtacgggccc caaggccggc ggcccgttat atttataccg attgttgtcg
     3961 gtgcagcctg cggccctgcc gccatgggcg gacctgtctg acggacttcc actgacacgg
     4021 gcgctggccg gtccaaccgg ggagagcaat acctatcagc ttttgccgcg aggagtggtg
     4081 ctttgcgtgg cggagaccgt cgaaggtgcg caagcccagt ggtcggccgt cgtcgaaacc
     4141 ggtaatcgcg cgctatttgt ggatactcag gcaacaaaaa cgtttcttgc gacgctcgat
     4201 ggcgtgcaac gtcaatctgt ccaaactgta tccgatgccg atatcgatca ggccgagttc
     4261 gacgctgtgc ttttcgaagg cgatagcgac gcattgcgcg aactgaaccg gcgcgtcgcc
     4321 tcgcgcgacg ggccgctagt ttccgtgcag ggcctgactt ccgacgcgtt gtcggcgggc
     4381 gcgctgtata tgagggatcg cctgctgtcc gaacgatcac taagcgtgaa tacggcggca
     4441 gccggcggga atgctcacct gatgtcgata gggtaactga caaaacacga gatgtttata
     4501 tgaacgtccg gcagctcgaa gccttttgca aggtcatgga agtgggcagc atgtcagaag
     4561 cagcgcgtct tctgggcatt tcacaaccgg ccgtctcgaa atcgatccgg ctgctcgagc
     4621 aggcgttgaa gttgcgttta ttcaagcggt cgggcgatcg cctttatccg agcatggaag
     4681 cgcagcggct gtaccccagc gcccggcgta tttttgacga aatacaaacc acttttgaac
     4741 ttggcaagaa actgcaggcg gcggaagtcg gaacattgaa gattgcggcg acgtattcgg
     4801 tgaccgccgc gtacgtgacc gaagcgattg aagcttttca caatcgccgg ccccttatcg
     4861 acatccagtt catggccttg ccgcctcgtc agatcatcga ccttgtcaga acatgtgaaa
     4921 tcgacgccgg acttttgtac gagcctattt cggtgcctaa ggtacagaca atacctttat
     4981 gcgatatcga gatgatctgc gcactgcctt tgctgcatca tttgggcaac aaagatgtca
     5041 ttgacagccg ggacctgacc aatgaaacgg tgatttcctt tagtggggag gcctatggcg
     5101 gcgacttgct taaacgtcaa tgcgaggcgg ccggcgcctc ctggaatgta gctatcgccg
     5161 tcaatcagac tgtggtcgcg atgaacatgg ccgcagcggg cattggcaca gcggtcattg
     5221 attcgcttgc attgagcgat agcgtttcag gcaaggtcat catcaaaccg tttcggccgc
     5281 ttacggtgct tcatgtcagc gccattgtcc cttcagaacg gcctctctcg cagttatgca
     5341 aggaatttat cgacattctt gccaacgttg tcaaacgtca ttccgaacag tcgtcggggt
     5401 tccacaaggt agcggcgtca cccataactt tcagtgcgca ggcaagactt caaaagtcat
     5461 aggttagaca aaaacaaatc gtgcctccta gaattccgtt gacacataag gagcgggttc
     5521 aatggacttt tcaatggtgg aagcgcaccc cgaggtacgt gaggcgatcc gggccctctg
     5581 tgcccctttt gacgaccagt actggcgcaa gaaagacaaa gaaggcacct tcccggagga
     5641 tttttaccgc gcgatcgccg agggcggctg gctggggatt gcggcacccg aaagttatgg
     5701 tggatcgaat ttggggatga ccggcgcgct tgccatgctg cagactattg ccgaatccgg
     5761 cgccggcatg aacggtggca catcggtcaa aatcaatatt ttcggactta acccggtgat
     5821 caaattcggt agcgacgagc aaaaggaacg gatgattccg gcaattattt ccggtaaaga
     5881 aaaaacctgt ttcgccatca ccgagcccga tgtcggttta aataccactc aactgaaaac
     5941 gcgcgcagta cgcgatggcg atcattatgt tgtgactggc gccaagacat ggatttcgac
     6001 cgcccaagta gccgaccgca tgctgttact ggcgcgcacc acaccgctgg atcaagttga
     6061 aaagcccacc gacggactga gcctgttctt gacgccgctt gatcgcaact acgttgaagt
     6121 ccgtgaaatt cagcgtatgg ggcgctgtgc cgttgacgca aaccagatgt tctttgatag
     6181 tcttcccgtg ccggcggccg atcgaattgg agaagagggt caaggtttca ggtacgtatt
     6241 cgacagcatg aatcccgagc gcatggtcgt tgccgctgaa gcggtgggca taggccgggc
     6301 ggcgttgcgc aaagcggctc gctacgccaa tgaacgggtg gtgttcggtc gccccatcgg
     6361 tcaaaaccag ggggtgcagc atccgctggc ccgttgctgg atggatctgg aggcggccaa
     6421 tttaatggcg tcacaggcgg cctggttata tgatcgcggc gaacactgcg gcttgcaagc
     6481 caattcggcc aaatatttgg cgggcgaggc tggcttcaaa gcgtgtgaag cggccatgct
     6541 cactatgggt ggaaatggct ttgccaaaga gttcgatgta gagcgatatc ttcgggacat
     6601 catggttgcg cgcatcgcgc ccatcacagc ccagttgatc ttctcatacg ttgccgagaa
     6661 ggctctgggg ctgccgaagt cctattgaaa cttgattcgc cgattgttcg cggcaagtaa
     6721 gcgcactcgc ggcgaattac cactacggga tcaccatatg gaccgctctt ccgcacctgt
     6781 cagcccggct tcatctgcat ccgatgacgt tgtcctctat tcattatcag agggactggc
     6841 ggttatcacc ctgaatcgcc ccgacaaact caacagcctg aacgccgcca tgcacagtcg
     6901 cttgcgcggc ttgctggacg agatttccgc tgagccttct gtgcgggcag tactcttgac
     6961 cggttcaggg cgcggatttt gcgccggtca ggatctgggt gaacggaaac gggaaccggg
     7021 cgaaccggcc cccgatttgg gccggaccat cgagaaaaac tggaacccgc tggcacgggc
     7081 tctgcgctac ctgagagtgc caattatctg cgccgtcaat ggggtggccg caggcgccgg
     7141 cgcgaatatc gcgctggcct gtgacgtggt gatcgccgcc cggtcggcaa gtttcgtgtt
     7201 gtccttttcc aggataggcc tcgttccgga tgccgggggc acctactatc tgccccgtct
     7261 ggtgggcgag gcgcgcgcga tgggcatggc tctgtttggc gagaaagtca gcgcagccga
     7321 tgccgctggc tgggggctta tctggcgctg cgtcgaagat gatgagctgg ccgaacaagc
     7381 gcgcaaactg gccatgtccc tggcgcaggg ccccacgctg ggcctcgggt cgcttaagca
     7441 aattatgcgg gcatctctgg atcattcgtt cgaagagcag cttgagcttg aacgtcgcac
     7501 acagcaacga tgcggagcca gcgaggatta ccaggagggc gtcgccgcct ttatggaaaa
     7561 gcgtgcacct cagttcaagg gaaggtgaaa tacaaagcat gtcgcccgcc aatatgtttt
     7621 ccgctacagc gggattcggc gcagacggtt cgccatctgt cccggatcgt gagccaatcc
     7681 aggtgctgga tctttatccg ccgcacggcg ataccttatg ggggctggta aaaagtcgcg
     7741 ccgccatcct cggtgatcgg ccatttattt tctttaatgg cgttcagtta agctgggcgg
     7801 cgtttcgcga caaggtcgtc cgtgttgcgc gcctgcttcg cgcgcatggg gtcgagccgg
     7861 gggatcgcgt tgggatcatg gccgacaact ccgacgacta cgtcgtgatt ttttttgcca
     7921 ccgcgtatgt cggcgccatc ttcatcccca tcaatcccag actaactgta gccgaaactg
     7981 cctacattct gaataacgcg cagcctgcgt tggtgttttg cgtgccggca tctgatgcaa
     8041 ttctgcgtgc ggcgtgtcag tcgctggagc cgttgccgca cattgcgtcc cttgcaaact
     8101 cctgggaggg tttggctgcg gcgggctctg ccggagatga tgcggtcaat tcaggcgtgg
     8161 attcggtgga atctgccgca ccggaggata cggccctgat tctgtatacc tctggaacta
     8221 cgggttttcc caaaggcgtt atgcacagtc agcgcagcat tgtcatggcg ggcgaatact
     8281 ttgtggagcg cttgcatttg caacccgaag atcggctgct ttgcgttttg cccttctttc
     8341 atatcaacgc tctgatttac tcgctgctcg gcgcggtggc cgcagggtct tcgctggtga
     8401 tcgcgccgcg cttcagcgca tcggagttct ggacgcttgc tgccgaaagc ggcgccacac
     8461 aggtcaacat catcgcaaca gtggggcgca tcctcatgcg ccggcctcgt tcggaattcg
     8521 ttgcggggca tcatattcgc aagctttacg gcgcgcccat caccgcagac atttacagcg
     8581 cctttcagac cgagttccga atccccgtgt tgatcgaagg gtatggcttg accgaggcgc
     8641 cggggatatg cagcaaccca tttaacgggc cgcacaaggt cggcagtatc ggcattccga
     8701 cgcatcatcc cgatcccggc cgtccttacg cccagatgcg cgttgcagac gagtccggcc
     8761 gcgaactccc tcgtggttca acgggggagt tgctcgtgaa tagccctatc gccatgcaag
     8821 gctactatcg cgataccgcg gcgagcgatg cggcattttt aaatggctgg tttcgcacgg
     8881 gagacctggt caggcaagag gaggacggtt actacacgtt tatcgaacgc caaaaggaca
     8941 ttattcgccg ccgtggcgag aatatctcgg gcgccgagct tgatcggatt atcggccagc
     9001 acggcgaggt gcagacggtc gccgcgatcg ctgtcgggtc agagttggga gaggacgata
     9061 ttctggtggc cgtgatcgtg aagcaaggat ccacactgac cgagcgcgag ctgcgcaaat
     9121 ggtgcgagca acgattgaat tcggccaagc ttccgcgata catcgccttc gttgattcat
     9181 tgccgtatac cccaagtcat cgcatcgcca aatttcaatt gcggcgtgat ccagaccttc
     9241 ggcagcgcgc tgtcgagttt cccgatatgc cagtagttcg ggtttagctg tagccagagc
     9301 cggcaacatg gagaggccgg tttgcgttcc aaccaaagga gacaaaaaat gaaaattcgt
     9361 aacgttgttg cggtccttgc gggtctgggt atcacaatgt tggggctgtc ggctactgcg
     9421 tcggccgcag ccgggggtgc cggtgcgttt ccaacgcact cagttcgcat gctggtaggc
     9481 tacccgccgg gaggcaacgt cgatattgcc gcacggatca tagcggcgga gcttggcaag
     9541 aaactgggtc agtccgtcgt cgtggacaat cgcgcaggtg caagcggcca aatcgccgcg
     9601 gctgccaccg cccaggcggc gcccgacggc tacaccatcc ttcttggtgc ttcgccagaa
     9661 ctggccattg tcactgcttt gaaaaagaaa gtgccttacg accctttgaa agatctgcag
     9721 ccgattagcc tggcgactca ggtgcctttc gtcctggtcg tcaatccttc tgtcccggcg
     9781 aagacggtta aagagctaat cgcgtatgcc aaggcgaacc ccggcaagct gaattacgcg
     9841 tcgtttggca acggatcatc caatcatttg tttgccgagt tgttcaaatc cagagccgga
     9901 gtcaagattg agcacattcc ttataaagga ggcggtcctg ccatgacggg tttgctggga
     9961 ggccaggtgc agatggaatt cgaaagcctg ggcgttgtgc tgccgcatgt ggaagccggg
    10021 aaactgcgtg cgctggcagt ggcgatgccg gcgcgttctt cgctcgcccc tgcgatcccc
    10081 actatggctg aagccggctt gccggggctg gaaggaggta cctggaatgg atttgtaggg
    10141 ccggcgggga tgtcgccggc catcaccgcc aagttaagcg aaaaaatcaa tgaagcgatg
    10201 cgtgagcctg ccgtcagaaa gcagttgatt gagaaaggtc tgattccgtc tgcggactcg
    10261 cccaaggaat ttggtgcgtt tattgcttcg gagattaaac gatggagccg ggttgccgag
    10321 gcagcgaaca tacacatcga ttaaggctgg cgccttaatg atcggcaagg attcagcccc
    10381 agatttgtcc gagcactcgc aagaagttgc cgcctgcaat ggcggcgact tcgtcggaac
    10441 tgaagccgcc tctttcgagc gcgtcgataa tatggggaaa ttgagcaggc gtgggcgtct
    10501 cgggtgggta gcggcccgct gacgaattgc cgaatgcttc cgcgtatttc ccgcccgttt
    10561 cgggataggc gcctttcgaa agctccaata ttgagcgcac actctcctcg ctttgcgcca
    10621 ccgagaagtc ggtgccaatg ccgacatgct caacgcccac cagatcggcg acatattgcg
    10681 catgccgcac aaaattggcc aggctgggcg gatgctttgg attgttgtcc caattaagga
    10741 aaccgtgtat tgacgtacca ataatgcctt gtgttttagc cactgcgcgt atggccttat
    10801 cggacttgtt ccggatctga tcgtccagac cttttgcgtt cgcatgggta acgagaacgg
    10861 gcttgcaaga ggcgtcggca gcctcgattg tggtacgttc accgcaatga gacaggtcaa
    10921 ttgcaatgcc tacccggttc atttcggcta ctaaggtctt gccaaatcgg gtcaggccgc
    10981 ccccacgttc ctccaggcac ccgtcgccca cttcgttcgc ctcgttatag gtaagctgca
    11041 cgacacgcag gcccagggcg tgaaacgccg tgaccctgtc aagctcggtc ccaaaaggca
    11101 gcgagttctg ccaacccatg atcagaccgg ttcggccttg cgcctgcgcg gcgaggatgt
    11161 cgccggccgt gcgtaccaga cgccagccgc tatcgggcgc ggcaaggcgt ttgtgccact
    11221 gaccgaaagc tgtaaaggtc tcttccagac cccaatgcat gtgcgtgaca gtaatgttgg
    11281 ctgctgttac gccgcccgcg cgaaacggtg caggatcgcc gtcgcaaaaa tagaccagac
    11341 cgtcaatgac caatttacgt gttgcgggtt tctcgctcat gattcagcca ctttcctttt
    11401 aacttttaag atcgaggtat atgaacatag gcagggtgca gttgctgcgt tgcgccgcga
    11461 cgtgttgcga ataaaaatgg tagcggataa agtgcggtga tgcaaaagat tagttcgacg
    11521 tcgaaatcag gtgaatagaa tcggggtggc gtaatgtcca gcggatcagg tacggtcgcc
    11581 gttgtgggcg cgggtattgt cggggtgtgt atcgcttacg aattgcgcaa gcgtggtgcg
    11641 gatgttctgc tgattgatcg tgcagagcct ggccgaggct gcagcttcgg taattcgggc
    11701 gccatcagcg ccagttccat tgctccgctg gccatgccgg gcatattggc ctcggtgccg
    11761 cgcatgctgt gcgatgccaa tgggcccttg tatttaccat ggcgttattt gcccaaggcg
    11821 ttgccctggc tggcccgttt tgtctggtca gcgcggcccg ggccggttaa tagggcggta
    11881 gccgatcttg ccgcactcca tgaaggggcg gttaatcgac acgtggccat ggcgcgcgaa
    11941 gtcggcgtgc ccgaactcat cttgcagcgc ggacatctgc atctttatcc cgatgagagc
    12001 gcgcttgcga aagatgcagc gggatgggac ctccgtcgaa agtacgggtt tcagtttgag
    12061 cgccttgagc gcgatggcat tattgccctg gaacctgatg tcagcccgcg ttatcgggtg
    12121 ggaatgttcc ttgccgatca cgcaacggtc aggaacccat ttcgctacgt gcaagccatt
    12181 ttccataaat atcttgagat cggcggccgc gcgttgcacg atgaggcgca agcgcttgcg
    12241 ccagagaaca atggcgaatg gcggattggg ttgagcgcgg ggtcgcagac ggtggaaaag
    12301 gtggttgtgg cgg
//
LOCUS       DATCXU010000128         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_467274,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000128 DATCXU010000000
VERSION     DATCXU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467274"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VIR04_02815"
     CDS             <1..280
                     /locus_tag="VIR04_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY9459713.1"
                     /translation="ELMGGLPSFTAIAVAATGIAGAVLAGGLFKLLRIGDPAIEGFAL
                     GLTAHAIGTARALKDSQTSGAFAALGMGLNGVATAIFVPAALAVLDLF"
     gene            333..815
                     /locus_tag="VIR04_02820"
     CDS             333..815
                     /locus_tag="VIR04_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY9459714.1"
                     /translation="MPSFDVVSQVDKHELVNAVDQAERELANRFDFKGVDARFELEDF
                     VVNQSAPSVFQLDQMLDILRGRLSARGIDVRCIELAEPLQNVSGARRKITIKQGIEQA
                     VSKKLIAAMKEAKLKVQAQINGDKLRVSGKKRDDLQAAIALLRKTDVDLPLQFENFRD
                     "
     gene            complement(825..1586)
                     /locus_tag="VIR04_02825"
     CDS             complement(825..1586)
                     /locus_tag="VIR04_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459715.1"
                     /translation="MNLQAIRAIYLFEMARTWRTLLQSIIAPVISTSLYFVVFGAAIG
                     GRISEIGGVSYGAFIVPGLIMLSLLTQSISNASFGIYFPKFTGTIYEIMSAPISYFEI
                     MLGYVGAAATKSIILGVIILATAGFFVPLRVDHPLWMLLFLVLTSITFSLFGFIIGIW
                     ADGFEKLQLVPLLIVTPLTFLGGSFYSIDMLPPLWQTVTLFNPVVYLISGFRWSFYGI
                     ADVSVAISLIMTLAFLALCLGVIAWIFKTGYRLKS"
     gene            complement(1583..2509)
                     /locus_tag="VIR04_02830"
     CDS             complement(1583..2509)
                     /locus_tag="VIR04_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459716.1"
                     /translation="MPPIISVSSLGKTYASGFQALKNINLEIHQGEIFALLGPNGAGK
                     TTLISIICGLTNPTEGVVLADGHDIIADYRAARSKIGLVPQELTTDAFETVWDTVSFS
                     RGLFGKRANPGHIEKVLRDLSLWEKKDNKLITLSGGMKRRVLIAKALSHEPQILFLDE
                     PTAGVDVSLRKEMWQMVRALRASGVTIILTTHYIEEAQDMADRIGIINKGEIILVEGK
                     NELMRKLGKKQLMLTLQTRLDNIPAPLETYRLDLAAEGDRLIYTYDTQHEQTGITNLL
                     KDLNDLQITVKDLQTKQSSLEDIFVHLVSEPR"
     gene            2631..>2844
                     /locus_tag="VIR04_02835"
     CDS             2631..>2844
                     /locus_tag="VIR04_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY9459717.1"
                     /translation="MDASVLRLLTPDEMSRADQATVALGASGKDLMEAAGKAVADAVC
                     KRWARQPVVVLCGPGNNGGDGFVVARH"
BASE COUNT          666 a          708 c          819 g          651 t
ORIGIN      
        1 tgaattgatg ggtggtttac catcttttac ggctatagcc gtggccgcca ctggcattgc
       61 cggagcagta ctggccggtg ggctgttcaa gttgctgcgt attggagatc ccgcaatcga
      121 aggttttgcg ctaggtttga cggcccacgc aatcggcact gcccgcgcat tgaaggatag
      181 tcagaccagt ggcgcgtttg ccgcattggg aatgggcctg aatggcgttg ctacagcaat
      241 cttcgtcccg gcggcgctgg cggtactgga cttgttttag aatattttcc aattgattaa
      301 ttgaacaaac attacccaac aggatttacg ctatgccttc ttttgatgtg gtctcccagg
      361 tcgacaagca tgaactcgtc aacgcggtcg atcaggcgga acgggaactc gcgaatcgct
      421 ttgacttcaa gggcgtggac gcccggttcg agctggaaga ctttgtggtg aatcagtccg
      481 cgcccagcgt gtttcaactg gatcaaatgc tggacatttt gcgcgggcgc ctgtcggcgc
      541 gcggaatcga cgtgcgctgc atcgagttgg ccgagccttt acagaacgtg agcggcgcgc
      601 ggcgcaagat aacgatcaaa cagggtattg agcaggccgt atcgaaaaaa ctgatcgcag
      661 ccatgaagga ggccaagctt aaagtgcagg cgcagattaa cggcgacaaa ctgcgcgttt
      721 ccggaaaaaa acgcgacgac ttgcaggcgg cgatcgcgct gctgcgcaaa accgacgtcg
      781 atctcccact gcagtttgaa aattttcggg attagccgtt caatttagct cttaaggcga
      841 tagccggtct tgaaaatcca ggcgataacg cccaggcaca gcgccagaaa cgccagcgtc
      901 atgatcaggc tgatggcgac actgacgtcg gcgatgccat agaagctcca gcggaaaccg
      961 ctaatcaggt ataccaccgg attgaacagg gtgactgtct gccatagcgg cggcaacatg
     1021 tcgatcgaat aaaagctgcc gcccaaaaac gttagcggcg tcacgattaa aagggggacc
     1081 agttgcagtt tttcgaaacc gtcggcccag atgccgatga tgaaaccgaa cagactgaat
     1141 gtgattgaag taagcaccaa aaaaagcagc atccacagag gatggtccac gcgcagcggc
     1201 acgaagaatc cggcggtggc caaaataata acgcccagaa tgatggactt ggtcgccgcg
     1261 gcgcccacgt agccaagcat gatttcaaaa taagaaatag gcgccgacat gatttcgtaa
     1321 atggtgccgg tgaactttgg aaagtaaatt ccgaacgatg cgttcgatat gctttgtgtc
     1381 aataatgaca gcatgatcag gccagggacg ataaatgcgc cgtaggagac cccgccgatt
     1441 tcgctgatgc gcccgccaat ggcagcgcca aacaccacga agtacaaaga tgtcgaaatg
     1501 acgggtgcaa taatgctttg cagcaacgtg cgccaggtgc gcgccatttc gaacagatag
     1561 atcgcccgga tcgcctgcaa attcatcgtg gttcgcttac caaatgaaca aaaatatctt
     1621 ccagcgagct ttgtttggtt tgaaggtcct tgacggtgat ttgcaaatcg ttcaggtctt
     1681 taagcaagtt ggtgattcct gtttgttcgt gttgggtgtc gtacgtgtag atcagcctat
     1741 cgccttcggc ggccagatcg agccggtatg tttcgagcgg ggcgggaata ttgtccagcc
     1801 tggtttgcag cgtcagcatc aactgtttct tgcccagctt gcgcatcagt tcgttttttc
     1861 cttcgaccag aatgatttcg cctttgttga tgatgccgat ccggtcggcc atatcctgag
     1921 cttcttcaat gtaatgagtg gtcaggataa tggtgacgcc gctcgcacgc aacgcccgca
     1981 ccatttgcca catttctttt cttaatgaaa cgtcgacccc cgcggtgggc tcatccagaa
     2041 aaagaatttg aggttcatgc gataaagcct tggcaattaa tacgcgccgc ttcatgccgc
     2101 ccgacaaggt aatcagctta ttgtctttct tctcccatag agacaggtcg cgcagcactt
     2161 tttcgatatg ccccgggttt gcgcgcttgc cgaatagtcc gcggctgaaa ctgaccgtgt
     2221 cccagaccgt ttcgaatgcg tcggtggtga gttcttgggg gacgagtcct attttggagc
     2281 gggccgcacg ataatcggca atgatgtcgt ggccatcggc cagcacgacg ccctcggtcg
     2341 gattggtcag accgcaaata atgctaatga gcgttgtttt ccctgctcca ttggggccca
     2401 gaagcgcgaa aatttcgccc tggtggattt ccagattgat atttttcagg gcctgaaagc
     2461 ccgaggcata tgttttgcca aggctggaga cggaaataat aggaggcatg gaaaaattgt
     2521 gagatgtcta tcaggcaagg ccccgaatcg gtacggcgag ggggttcagc atagcatgcc
     2581 ggattggggt gcgtgctaga ctttaattcg ttccctgaag ggtggtgaag atggacgcaa
     2641 gtgttctcag gttgctgacg cccgacgaaa tgagtcgagc cgatcaggct accgttgccc
     2701 ttggcgcaag tggtaaagac ctgatggaag cggccggcaa agccgtggcc gatgccgtat
     2761 gcaagcgctg ggcgcgccag cctgtggtcg ttttgtgcgg acccggcaat aacggcggcg
     2821 acggttttgt cgtggctcgg catc
//
LOCUS       DATCXU010000129         9056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_47420,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000129 DATCXU010000000
VERSION     DATCXU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47420"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VIR04_02840"
     CDS             complement(<1..810)
                     /locus_tag="VIR04_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY9459718.1"
                     /translation="MKINAVVLRKVGVPAPYAQSKPLQLSQVELDPPGFGELLVKIKV
                     AGLCHSDLSVINGDRPRGVPIVLGHESSAEVVETGPGISDLKAGDHVVTVFVPSCGSC
                     FPCMEGRPALCEPGAAANTDGTLLGGERRLHIGGEYLNHHTGVSCFAEYAVVSRRSCV
                     KINTEITHREAALFGCAVLTGAGAVINRARVRAGDTVAVVGLGGVGLSALLAAVASGA
                     RRVVAVDLSDDKLNLAKQLGATHLVNPKNIKTDADLLDAAGGRVDYGFDMAG"
     gene            complement(832..1482)
                     /locus_tag="VIR04_02845"
     CDS             complement(832..1482)
                     /locus_tag="VIR04_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9459719.1"
                     /translation="MLEIKNLSVRFGSIEILRNIHLSVPQGAIVGLLGRNGAGKTTTL
                     RTIMGLVKPEAGSLTFDGIDLTRVAAYGRTAMGFGYAPEDRRLIPDMTVEENLSLPAW
                     ALKWKDIKGRLDGVYKLIPEVREFGPRRAMQLSGGQQKLVAIGRALVTGSRLLLLDEP
                     FEGVAPALSKRIAEVIGTLRGAGLTVVLSGADLQHASSVLDTVYRIDRGQMAASAK"
     gene            complement(1475..2233)
                     /locus_tag="VIR04_02850"
     CDS             complement(1475..2233)
                     /locus_tag="VIR04_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9459720.1"
                     /translation="MKTTSAFIQATGLQKSFGSVTPARNITVGIDQRSVVGLIGANGA
                     GKTTFINMLTGYLKPDKGEIVFRGKNITGLAPRKITRMGIARSFQIPQLFVSQTTREN
                     IDIALSISGLSRALADESLDRLGVLEFADMPCGVLPEGIRKLLDIALAMVCKPEVLLL
                     DEPTSGVAAEEKFDVMSRVLDATRQLGVTVLFVEHDMEIVRRYSDRVLAFYDGAIIAD
                     GNAVEVLANSQVLEFITGETSVAVSSNQMVAGNA"
     gene            complement(2230..3207)
                     /locus_tag="VIR04_02855"
     CDS             complement(2230..3207)
                     /locus_tag="VIR04_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459721.1"
                     /translation="MTSGFRFSDHWFPISCAVVLAAVALILPQWQFLISIALSKGIVA
                     LGLALLLRAGLASFGQALFFAVGAYTVGMLMNFLGVKELFTLLAMASAVSAALAWALG
                     FLLSRYRDIFFAMLTLALSMVMYGILINNPGLGGSDGFNVSPPLLGLGAGGQTISGTG
                     LFALVYCAAVVCVLLVHWFMRSVPGRLGPAIKDNEIRVEYLGASARANIHLVYLIAGA
                     LAGIGGALSAFNVGHIDPDMAFWTTSGEFLFIAVLSGTRSAYAPFLGALLLEVAQAVA
                     YRYAPNTWQIAMGTVMLVVIAFLPGGLTSLRLTRATGSASKSVDKDMAV"
     gene            complement(3204..4100)
                     /locus_tag="VIR04_02860"
     CDS             complement(3204..4100)
                     /locus_tag="VIR04_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459722.1"
                     /translation="MSQLYAVLVDGAMYAAWLFLVALGLTLVYGVLKILNMAHGSLYA
                     LGAYAGVTLIGPWVAHGGNLYVSYLLLVVAAMVAGAAVGIIMERGVLRRFYGQDPVVL
                     LLVTYALFLIMEDAMKLVWGVDSYSVSEPYGFLGNFSMGDLSYPNYNFIILGMAILAG
                     VGLTSFLSYTRTGRLLRAVIHDREVSQALGIRVNRYFVTTFVVGAVLAALGGALTAPT
                     VSVAPGMGVEVIVLTFAVVVIGGLGSLPGAALGALLVGLVRAAAVHYWPQGELFSIYI
                     VMALVLVFRPKGLFVLAEARKI"
     gene            complement(4213..5499)
                     /locus_tag="VIR04_02865"
     CDS             complement(4213..5499)
                     /locus_tag="VIR04_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459723.1"
                     /translation="MKTNINFGYKPLAVALACGALLCSSAVMAQTKPIKIGIVTFMSG
                     PAAGPFGVPARQGAEVTAAELNAGKVPAPYATKGFGGTPVEFVYIDEAGSTSKQVSEY
                     RNLINQGVDYVIGYTSSGNCLAVAPVAEEMKKVTLFFDCGTPRIFEDSSYKYVFRPVS
                     NATMDSVGAARYVVDLKPNLKTYAGINQNYAWGQDSWSDFENAMHVLAPSAKQVSSQM
                     PKFGAGQYNAEISSLLAAHPEVVFSSFWGGDLESLLVQAGPRDLFKKSLTVLSTGETA
                     AHGKARLPDGVIIGARGLYGVFAPDNALNHWLRDAYTAKYADTPSYPSYKMTQSIFGL
                     KAAYEKAQKANGDKQPTPDQVIAAFEHLSFDSPAGMINMALGKGHQGAQGVAYGMVKN
                     VDGKVTVVNVKHYSAQEVLPPDGVKSADWIKAGMKH"
     gene            5729..6538
                     /locus_tag="VIR04_02870"
     CDS             5729..6538
                     /locus_tag="VIR04_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY9459724.1"
                     /translation="MIKRRSNTSIASSDKKPIKTTSPAVDRAVHILDVIAQSAEPFSL
                     ADLARETAFPKSTLHGLCKTLIRLRLIKQLPNGAMTLGPHVMIWANAFPSQTNITQEF
                     HAVWEESEAFPQETVTLSVLDGDDVIYIACHNGSRPLGVTFRIGMRLPAAYTATGKAM
                     LSTLPNKELKEFFANGWPAPLTKASVANLDDLSREIEQCRRIGYSIDNGQTRDGMICF
                     GAPVFNATESRAVAGVAVSFLATEIDSKTRKRIGQKMCALAERLSKKLNAG"
     gene            complement(6566..7774)
                     /locus_tag="VIR04_02875"
     CDS             complement(6566..7774)
                     /locus_tag="VIR04_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459725.1"
                     /translation="MINSTTTVDKPPLAALKLTPVERRASVALALLFAARMLGLFLLT
                     PVFAVVANTVPGGDNPAKVGLALGAYGLTQAIMQIPLGMASDRYGRRSVIIFGLLLFI
                     AGGVICALAADVNWIIVGRIIQGVGAVSAAITAWVADATRPEVRTRAMALVGGSIGIS
                     FAVSLVLSPLLVGEFGLSGLFWAISLLGFACLLITAFVVPVVPQEQAPIVQVRPRDVL
                     GHVDLLRLNFGVFCLHFILMSIFMVAPGLLQHLGGYDSSHLWEVYLPVILLSFVLMVP
                     AVFYTETRRAHKVALEASVAALVVVLALMPWASHSFYTLVILLVGFFIAFNILEALQP
                     SLVSRVAPPNFKGLALGFYNTAQSLGVFGGGVVGGLLAAHQGQSKVFLVASALAFVWF
                     VTARGFKAVS"
     gene            7870..>9056
                     /locus_tag="VIR04_02880"
     CDS             7870..>9056
                     /locus_tag="VIR04_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY9459726.1"
                     /translation="MQAIRIRGARTHNLKNISIDIPRHQLVVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSTYARQFLQLMDKPDVDLIEGLSPAISIEQKSAGHNPRSTVGTIT
                     EIHDYLRLLYARVGTPYCPEHGLPLQAQSVSQMVDKVMTWTPDTRLAILAPIARSRKG
                     SFEDECASLQAQGFVRLRVNGEMIGIDQLPPLKKTEKHDFDVVVDRLRIKPESQQRLA
                     ESFETAINLAEGRVIALNMDTGEEHNFSSRYACPICHRNLTELEPRLFSFNNPVGACP
                     ECDGLGQVETFDPKRVVAFPELSLAAGAINGWDRRNAFTHTLLTSLAAHYEFDIETPF
                     EELDALVQQHVLFGSGEEEIAFLYLNEKGHATVKKHPFEGIIPNLERRWLETDSTAVR
                     EELG"
BASE COUNT         2289 a         2744 c         2337 g         1686 t
ORIGIN      
        1 gcccgccata tcgaaaccgt aatcgacccg cccgccagcg gcatcgagca aatcggcatc
       61 ggtcttgatg tttttagggt ttaccaaatg cgtcgcgccc aattgtttcg ctaaattcag
      121 tttatcgtcg cttaaatcaa cagcaacgac gcgacgcgca cccgacgcca cggcagccag
      181 caacgcgctt aaaccaacgc cgcccaatcc aacgactgcc acagtatcgc ccgcacggac
      241 tcgtgcccga ttaatgacag cgcctgcgcc agtcagcacg gcgcagccga aaagagccgc
      301 ttcgcgatga gtgatttccg tattgatttt gacgcacgaa cgtctggata cgacagcgta
      361 ttcggcaaaa cacgacacgc ccgtgtgatg attcaggtat tcgccgccaa tatgaagacg
      421 gcgttcgccg cccagcaagg ttccgtccgt gttggctgcg gcgcccggct cgcacagagc
      481 gggtcgacct tccatgcacg gaaagcaact gccgcaactg ggcacgaaaa ccgttacaac
      541 atgatcgccc gccttaaggt ccgagatgcc cggacccgtt tcgacaacct cggcggagga
      601 ttcatgaccc agcacaatcg gaacgccacg cggccgatcg ccgttaataa ccgacaaatc
      661 ggaatggcac aagcccgcga ccttgatctt caccaacagc tcgccaaacc ctggaggatc
      721 cagttcaact tggctaagtt gcaatggctt gctttgggca tagggcgccg gaacgcccac
      781 cttgcgtaac acaacagcat taattttcat gaattcgctc cttgcgaaaa gtcattttgc
      841 cgatgcggcc atctggccgc gatcaatgcg atacaccgta tccaggacag aactggcgtg
      901 ctgcagatcg gcgccagaaa gaacaacggt gagcccggcg ccgcgcaaag tgccgatgac
      961 ctcggcgatg cgcttggaaa gcgcgggggc cacgccctcg aaaggttcgt caagcaacaa
     1021 cagacgcgag ccggtaacca gcgcgcggcc aatggccacc aacttttgct gcccgcccga
     1081 caactgcatt gcgcggcgcg gcccaaattc gcgcacttca gggatcagtt tataaacgcc
     1141 atccagccgg ccttttatgt ctttccactt caatgcccag gccggcaaac taaggttttc
     1201 ctcgaccgtc atatcgggaa taaggcggcg atcttcggga gcgtaaccaa aacccatcgc
     1261 cgtccggcca tacgcggcca cgcgcgtcag atcgatgcca tcgaatgtca atgaccccgc
     1321 ttccggcttg accaggccca tgatcgtgcg caaggttgtg gtcttgccgg cgccattgcg
     1381 ccccagcaaa ccgacgatcg cgccctgtgg aaccgaaaga tgaatattgc gcaggatctc
     1441 tatggaacca aagcgaacac tcaaattttt tatttcaagc attgcccgcc accatttgat
     1501 tcgaagaaac cgccacggag gtctcgccgg taataaactc gagcacctgg gaattggcca
     1561 atacttccac ggcattgcca tccgctatga tcgcgccgtc gtagaatgcc agcacccgat
     1621 cgctgtatcg acgcacaatc tccatatcgt gttcaacaaa cagcactgtg acacccagct
     1681 gacgcgtcgc gtccagcaca cggctcatga catcgaactt ctcttcggcg gccacgccac
     1741 tggttggctc gtcaagcagc aatacttcag gcttgcacac cattgccagg gcgatatcaa
     1801 gaagttttcg tatgccttcg ggcaaaacac cacaaggcat atccgcgaat tcaagcacac
     1861 ccaatcgatc gagactttcg tcggcaaggg cgcgcgacaa acctgaaatc gacagcgcta
     1921 tgtcgatatt ttcacgagtc gtttgtgaaa cgaataattg tggaatctga aacgagcgcg
     1981 caatgcccat gcgggtgatt tttcgcggcg cgagccccgt gatatttttg ccgcgaaaaa
     2041 caatctctcc cttatcgggt ttaaggtaac ccgtcaacat attgatgaaa gtcgtcttgc
     2101 cggcgccatt ggccccaatc aggccgacaa cgctgcgttg gtcgattcca acggtaatat
     2161 tgcgcgccgg cgtcacactt ccgaatgatt tttgcaggcc tgtcgcctga ataaatgcgc
     2221 ttgtcgtttt cataccgcca tatccttgtc aactgacttg gacgcgcttc ccgtggcgcg
     2281 tgtaagcctt aggctggtca gtccgcccgg caagaacgca ataaccacca gcatgacggt
     2341 acccatcgcg atctgccagg tgttgggcgc ataccggtag gcgaccgcct gcgcgacttc
     2401 gagcagcaat gcgccaagaa acggcgcata agcgctgcgg gtgccgctta gcaccgcgat
     2461 gaaaaggaac tcgcccgacg tcgtccaaaa agccatatcc gggtcaatat gaccgacgtt
     2521 gaacgccgac aacgcgccgc ctattcccgc caatgcgccc gcaatcagat aaaccagatg
     2581 gatattggcg cgcgccgacg cgcccaggta ctccacccga atctcgttgt ctttaatcgc
     2641 ggggccgaga cggcccggca ccgaacgcat aaaccaatgc accagcagca cgcacacgac
     2701 ggccgcgcaa tacaccaagg caaacaagcc ggtgccggaa atggtctgac ctcccgctcc
     2761 aaggcccagc aacggtggcg acacattgaa accgtcgctg ccacccaagc caggattgtt
     2821 gatcagtatg ccgtacataa ccatggataa ggccagcgtg agcatggcga aaaatatatc
     2881 gcgatagcgc gacagcaaaa acccgagcgc ccacgccagc gcggccgaca ccgcactggc
     2941 catggccaat aaagtgaaca attccttgac gccaagaaaa ttcatcaaca tgccaacggt
     3001 gtaggcgccg acggcaaaaa acaaagcctg cccaaatgac gctaggccgg cgcgcagcag
     3061 cagggccagt ccgagagcca caatgccttt ggacaaagcg atggaaatca ggaactgcca
     3121 ctgcggcaag atcagggcaa cagcggcaag caccaccgca caacttattg gaaaccaatg
     3181 atcagaaaaa cgaaaaccgg aagtcatatc ttgcgagcct ccgccaatac gaataaaccc
     3241 ttcgggcgga agaccagcac aagcgccatt acgatataaa ttgaaaacaa ttcgccctgc
     3301 ggccaataat gcaccgccgc ggcccgtaca agtccgacca gcagcgctcc gagcgcggcg
     3361 ccgggcaatg aacccaagcc gccaatcacg acaacggcga acgtcaatac aatgacttcg
     3421 actcccatgc ccggggcaac cgacacggtc ggcgctgtca aagcgccgcc aagcgctgct
     3481 agcaccgccc ccacgacaaa ggtcgtaaca aaatagcggt tcacacgaat tccaagggct
     3541 tgactgactt cccggtcgtg aatgacggcg cgcagtaaac ggcccgtgcg ggtatagcta
     3601 aggaacgacg tcagacccac acccgcaaga atggccattc ccaaaataat aaagttgtaa
     3661 ttgggatacg acaggtcgcc catggaaaaa ttgccgagaa agccataagg ttcggacacc
     3721 gaataggaat ccacgcccca caccagcttc atcgcatcct ccatgatgag gaacagggca
     3781 taagtgacca ggagcaacac cacgggatcc tggccataga aacgccgaag aacgcctcgc
     3841 tccatgatga tgccgactgc cgctcccgca accatggccg ccaccaccag caataaatag
     3901 ctgacataca aattgccgcc gtgggcgacc caagggccaa tcaaagtcac gccggcatag
     3961 gcgcccaatg cgtataagct gccatgggcc atgttcaaga ttttcaacac gccgtaaacg
     4021 agcgtcagcc ccagagcaac aaggaacaac cacgccgcgt acatcgcccc atcgacgagg
     4081 acagcgtaaa gctgcgacat cccgttttct ccttaaatag atagtgcgct ggaaaagttg
     4141 ccacccagca aggcggcggc aaccgtccag cgcaatgtac ccaccgtcgg gccgtttcaa
     4201 cccgacaagg gatcagtgct tcatcccggc tttgatccag tcggcggatt tcactccgtc
     4261 cggcggaagg acctcttgag ccgagtaatg tttgacattg accacagtga ccttgccatc
     4321 gacatttttg accatgccgt acgccacacc ctgcgcgccc tgatgacctt tgcccaaagc
     4381 catattgatc atgccggcgg gcgaatcaaa ggacagatgc tcgaatgcgg caatgacttg
     4441 atccggcgtc ggctgtttgt cgccattggc cttttgcgcc ttttcgtatg ccgccttcag
     4501 accgaatatg ctttgagtca ttttgtacga cggatagctt ggagtatcgg cgtatttggc
     4561 cgtgtatgca tcgcgcaacc aatgattcag cgcattgtcg ggcgcgaaca cgccataaag
     4621 acctcgggcg ccaatgatga ccccgtcagg caagcgggct ttaccatgcg ccgcggtctc
     4681 gccggtggag agcacagtca gcgatttctt gaagaggtcg cgcggaccgg cctgtaccaa
     4741 taaactttcg agatcgccgc cccagaagct tgagaatacg acttccggat gcgcggccag
     4801 caggctggag atttcggcgt tatattggcc cgctccaaat ttgggcatct gcgaactaac
     4861 ctgttttgcc gacggcgcca acacatgcat ggcgttttca aagtccgacc acgaatcctg
     4921 gccccaggcg tagttctggt ttatacccgc atacgttttt aaattgggtt tgaggtcaac
     4981 cacatagcgt gcggcgccta cgctatccat cgttgcgttc gagacgggtc gaaacacata
     5041 tttgtagctc gagtcttcga atatgcgtgg tgtgccgcaa tcaaaaaaca gcgtgacctt
     5101 tttcatttcc tcggccaccg gcgccaccgc caggcaattg cccgatgagg tataaccaat
     5161 aacgtagtcc acgccttggt tgatcaaatt gcgatactca ctgacttgct tgctggtgct
     5221 gcccgcctcg tcgatgtaga caaattcgac gggtgtcccg ccaaaaccct tggtcgcgta
     5281 gggcgctggc accttgccgg cattgagttc ggcggcagtc acctcggcgc cctgtctggc
     5341 cggcacgccg aacggtccgg ccgccggacc agacatgaat gtgacgatgc cgatctttat
     5401 cggcttggtc tgcgccatga cggcgctgga gcataacaac gccccgcatg ccaaagccac
     5461 agcaagcggc ttatagccga aattaatatt tgtcttcatt ctacttatcc tttgataatt
     5521 gcacttctcg accaagccgc ccttcatgca tacagcggcg gcgctttcac tctttatcct
     5581 cagtcgcacc gttgcgttca ctatattgaa cggcattcta ccgatggaac aatggaaaag
     5641 taaaccggta attcccctaa gcggctattc gcaggcttcc gcgtatcata agagcgttga
     5701 gtgcccagat ttcccttccg ttttcctgat gataaagcgc cgatctaata ccagcatcgc
     5761 cagcagcgac aaaaaaccaa tcaaaaccac ctcgccagcc gttgaccgcg ccgtgcatat
     5821 ccttgatgtc atcgcacagt ccgccgagcc gttttcgctg gcggatctgg cgcgggaaac
     5881 cgccttccca aaaagcacgc tgcatggcct atgcaaaaca ttgattcgac tgcgtctcat
     5941 caaacagttg cccaacgggg cgatgaccct tggaccgcac gtcatgatct gggcgaacgc
     6001 gttcccgtcg caaaccaata tcacgcaaga atttcatgcc gtatgggaag aatcggaagc
     6061 atttccgcaa gagactgtca ccctatccgt gcttgatggc gacgatgtca tttatatcgc
     6121 gtgtcataac ggtagccgcc ctcttggtgt cacattccga atcgggatgc ggctacccgc
     6181 cgcgtacacg gcgaccggca aagccatgct cagcaccctc cccaataagg aattaaaaga
     6241 gtttttcgca aacgggtggc ctgctccgct taccaaggca agcgtcgcca atctggacga
     6301 tctgagccgc gaaattgagc aatgcaggcg cattggctat tccatcgaca acggccaaac
     6361 acgggatgga atgatttgct tcggcgcgcc tgtattcaat gcgacagaat ccagagccgt
     6421 cgcgggcgtg gcagtcagct tccttgccac cgagattgac agcaaaacca gaaagcggat
     6481 cggccagaaa atgtgcgcac tggccgaacg cctttctaaa aaattaaacg caggttaatc
     6541 cgccagattc cggcacagtc agcgcctaag aaaccgcctt gaatccgcga gccgtcacaa
     6601 accatacaaa cgccagggcg gacgccacca gaaatacctt cgattggccc tgatgcgccg
     6661 ccagcaggcc gcccaccact ccgccgccga acacgcccag ggattgcgcg gtgttgtaaa
     6721 agcccagcgc cagccccttg aaattgggcg gcgcgactcg ggacacaagc gacggctgca
     6781 gtgcttccaa aatattaaag gcgatgaaaa agccgacaag caaaattacc aaggtataaa
     6841 aactgtggct ggcccacggc ataagcgcca ggaccacgac cagcgccgca acagatgcct
     6901 ccaacgccac tttatgagcc cggcgggtct cggtgtaaaa cacagcaggc accatcagca
     6961 caaacgacag caaaatcacc ggcagataga cttcccataa gtgggacgag tcgtaccccc
     7021 ccaagtgctg caacaggccg ggagccacca tgaatatcga catcaggata aagtgcaggc
     7081 aaaaaacgcc gaaattcagc cgcagcaaat ccacatgacc caatacatcg cgcggtctga
     7141 cctgaacgat gggcgcttgc tcttgcggaa caacgggcac cacaaatgcg gtgatcagca
     7201 aacatgcgaa acccagcaag ctaatggccc agaacaggcc cgacaatcca aattcgccca
     7261 ccagcaaggg tgacaacact aaggaaactg caaatgaaat gccgatcgac cccccaacca
     7321 gcgccatggc ccgagtgcgg acttcgggcc gggttgcgtc ggcgacccat gccgtaatag
     7381 cggcggaaac cgcgcccacg ccctggataa ttcgcccaac aatgatccag ttcacgtcgg
     7441 ccgccaatgc gcaaataacg ccgcccgcaa taaataacag caagccaaaa ataatgaccg
     7501 aacggcggcc gtaacggtcg gaggccatgc cgagcgggat ctgcatgatc gcctgagtca
     7561 ggccataagc gcccagcgcc aggcccactt tggcagggtt atcgccgcca gggacagtat
     7621 tggccacgac ggcaaacacg ggcgtcaata aaaataaacc cagcatgcgg gcggcaaaga
     7681 gcagggccag cgccacgctg gcgcgacgct cgacaggcgt cagcttgagg gccgccagag
     7741 ggggtttatc gacggtagtg gtggagttga tcatgaaata ctcgttgtag ggccagcata
     7801 acgcacggat caagcgagcg cgctatagta gcgagttaac cttttgaaat cccgcaaata
     7861 aaacatttga tgcaagctat tcgcattcgg ggcgcccgca cgcacaacct gaaaaacatt
     7921 tccatcgata taccgcgcca ccaattggta gtggtaacgg gcctgtcagg atcggggaaa
     7981 tcgtcgcttg cgtttgacac actctatgcc gaaggacaac gccgttatgt cgaaagtctg
     8041 tcgacatatg cacggcaatt tttgcagctt atggataagc ctgacgtcga cctgatcgaa
     8101 ggattatcgc cggcgatatc gattgaacaa aaatccgccg gacataaccc ccggtcaact
     8161 gtgggcacga ttacggaaat ccatgactat ctgcgcttat tgtacgcgcg ggtgggtacc
     8221 ccctattgcc ccgaacatgg cctgccgcta caagcgcaaa gtgtgagtca gatggtcgac
     8281 aaggtcatga cctggacgcc ggacacacgc ctggcgatat tggcgcccat cgcccgatcg
     8341 cgcaaaggca gcttcgaaga tgaatgcgcc agccttcagg cgcaaggctt cgtgcggctg
     8401 cgcgtaaacg gcgaaatgat tggaatcgat cagcttccgc ctctgaagaa aaccgaaaaa
     8461 cacgatttcg acgtcgtggt ggatcgcctg cggatcaaac ccgaaagcca gcaacgcctg
     8521 gccgaaagct ttgaaacggc gatcaatctg gccgagggac gcgtcatcgc gctgaacatg
     8581 gacacaggcg aagaacataa tttttccagc cgctacgcct gccccatttg ccatcgcaat
     8641 ctgactgaac tggaaccccg cctgttcagt tttaacaatc ccgttggcgc gtgccctgag
     8701 tgcgacggcc tgggtcaggt cgagaccttc gaccctaaac gcgtggtcgc ttttccggaa
     8761 ctgagcctgg ccgccggcgc catcaacggt tgggatcggc gcaatgcttt tacgcacact
     8821 ctattgacaa gcctggcggc gcattacgaa ttcgacatcg agacgccatt cgaagaactt
     8881 gacgcactgg tgcagcaaca cgtgctgttt ggctcgggcg aagaagagat cgcgtttcta
     8941 tatttaaacg aaaagggtca tgccactgtc aagaaacatc ccttcgaagg aattatcccg
     9001 aacctggaac ggcgctggct tgaaacggat tcaaccgccg tgcgcgaaga actggg
//
LOCUS       DATCXU010000130         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_523993,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000130 DATCXU010000000
VERSION     DATCXU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523993"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIR04_02885"
     CDS             complement(<1..351)
                     /locus_tag="VIR04_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459727.1"
                     /translation="MANQPATQPAGKAASMRTVAAASAIGTTIEWYDFLIYATAASLV
                     LNTQFFPTHDPLVGKLLSIGTIGVGFFARPLGAIILSHFGDRLGRRSMLILTLVSMGS
                     ATALIGLLPTYATIG"
     gene            complement(509..976)
                     /locus_tag="VIR04_02890"
     CDS             complement(509..976)
                     /locus_tag="VIR04_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9459728.1"
                     /translation="MTSQASEHFQERLQGKLEPTKDGSIYVKPQDIGWSPSQFEGIDI
                     KVLYENKAAGELTCLLRWAPGTTLPFHKHPEIEQSWVLEGSFSDHDGICRAGEYVYRI
                     PGSMHETHSAEGCTILAIYRKPNIFRNSAGYDVEKKKQIKTQHEPSTWDAKAV"
     gene            complement(1121..1717)
                     /locus_tag="VIR04_02895"
     CDS             complement(1121..1717)
                     /locus_tag="VIR04_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459729.1"
                     /translation="MTKLARALATLLLATVSIPSVAQTARVRGEIEKFDHSVLTVKAQ
                     DSAIIDIKLDNDAAITAMTKASLADIATGSFIGTAAHPGGPNGELVALEVHVFAESMR
                     GTGEGHRPMTQGHTMTNATVENVVTAMNNRILTLRYNGGEQHIQIPDGIPIVMLSPSD
                     RNALKTGEHVSFKATKQPDGTITAARITVGVDGVIPPL"
     gene            complement(1812..2528)
                     /locus_tag="VIR04_02900"
     CDS             complement(1812..2528)
                     /locus_tag="VIR04_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY9459730.1"
                     /translation="MTDLSTFLVTKKWPPQHPDRIQLYSLPTPNGIKVSVMLEETGLP
                     YEPHLVSFQTHDQKSPEFLSLSPNNKIPAILDPDGPDGKPLPLFESGAILIYLSDKSR
                     QFMPRDAAGRYETIQWLMFQMGGIGPMFGQLGFFNKFAGKDYEDKRPRDRYVAEVKRL
                     LAVLEGRLSGRRWIMGDDYTIADIATFPWVRNLIGFYEAGDIVGISNFPNVLRVLDTF
                     VSRPAVVKGINIPPRDPASV"
     gene            complement(2563..>3287)
                     /locus_tag="VIR04_02905"
     CDS             complement(2563..>3287)
                     /locus_tag="VIR04_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9459731.1"
                     /translation="IDICHSIVDTLKKNLNLPDLHTKMVPVTPASRIPLIANGTVDLV
                     CGVSTNNAQRQTQVSFAPTTFVTATRFAALKKSHLKDLHDFNGKVVVGVSGSSNLRWL
                     TKTNDEQQLGMKIITAKGQPEGLFSVDTGRASAFFMDDVLLAGLVANSHSPNDWMISD
                     TAYTIEPYGIMEPKADEPFKTAVDNAVKAIMANGAINALYNKWFTRPIPPTNVNLNWP
                     ISPELKKAFEHPTDSPDPAVYK"
BASE COUNT          711 a          809 c          931 g          836 t
ORIGIN      
        1 gcctatcgtt gcgtaagtag gcagcaagcc gatcagcgct gttgcggaac ccatgctgac
       61 caaggtcagg atcagcatgg acctgcgccc caaacggtcg ccgaaatgac ttagaataat
      121 cgcccccagc ggtcgtgcga agaaccccac gccgatggtg ccgattgaaa gcaactttcc
      181 taccagggga tcatgggtcg ggaaaaactg ggtattcaag accaacgacg cggcagtggc
      241 atagataaga aagtcgtacc attcgatggt cgtgccaatg gcgcttgcag ccgccaccgt
      301 acgcattgac gccgctttgc cagcgggttg tgtagctggt tgattagcca tcatttgtct
      361 cctatgtggc ttgataaagg ccgcgcccgg ttgatggctt gctggctgtg gaaaagaatc
      421 gcgtcacttc cttaatgcca tgctgcgcta tttggaagtt ctccttgcac gcgatttcga
      481 ccaggcagtt caatctggcg cgatgctgtt aaacggcttt tgcatcccaa gtggaaggtt
      541 catgctgcgt tttgatctgt ttcttctttt cgacgtcgta gccggcacta ttgcggaaaa
      601 tattgggctt gcgataaatg gcgaggatgg tgcagccttc ggcactgtgt gtctcgtgca
      661 ttgagcccgg aatccggtaa acatattctc ccgccctgca gattccgtcg tgatcggaaa
      721 acgatccttc gagcacccag ctttgttcaa tttcggggtg tttgtgaaac ggcaaagtcg
      781 ttcccggcgc ccaccgaagc aaacacgtca gctcgcctgc cgctttgttt tcgtataaaa
      841 cctttatgtc aatgccctcg aattgagaag ggctccagcc tatgtcctgc ggtttcacat
      901 aaattgaacc gtctttagtg ggttcaagct tgccttgcag gcgctcttga aaatgttcag
      961 atgcttgact tgtcatggcg aattttgtcc aagtttaaat tcgattaaag gaaaagcggc
     1021 gtttataacg ccgcaaacat tgaagaaagc acgatagtcg tagtgctaat tggacaaatg
     1081 ataggataaa gttgtctggt agacaagtaa gtggttttac ctatagtggc gggatcactc
     1141 cgtcgacgcc aaccgtgatg cgcgcggcgg ttatcgtccc gtccggttgt tttgtcgctt
     1201 taaaagagac gtgctcgccc gttttaagcg cgttgcggtc gctgggggac agcatgacaa
     1261 tcggtatgcc atccggtatt tggatgtgtt gttcgcctcc gttgtaacgc aaggtcaaga
     1321 ttcggttgtt cattgcggtc accacgtttt ctacggtcgc attggtcatg gtgtgtccct
     1381 gcgtcatcgg ccgatgacct tcgcctgtgc cgcgcatgct ttcagcgaaa acatgcactt
     1441 ccagcgccac cagttcccca ttggggcctc cggggtgggc ggcagtgcca atgaaactcc
     1501 cggtggcgat atccgccagg ctggcttttg tcattgccgt gatggcggcg tcattatcaa
     1561 gtttgatgtc gatgatcgcg ctatcttggg ctttgacagt tagcacggag tgatcgaatt
     1621 tttcaatttc cccgcgtacc cgtgctgttt gggcaacaga cggaatagaa acagtcgcca
     1681 ataatagggt ggccagagca cgggcaagtt ttgtcatctt tttctcctgc aacaggcatc
     1741 aaggatagcc cggcggatcc gcgcccaatg caatacagaa ggcttagagg gtaatctcca
     1801 atctatctaa tctatacaga tgccgggtct cgtggcggga tattgatccc ttttacgacg
     1861 gccgggcgcg aaacgaatgt atcgagcacg cgcaatacat ttggaaaatt tgaaatgccc
     1921 actatatcgc ccgcctcgta gaagccaatc aggttgcgca cccagggaaa agtggcaatg
     1981 tcggcgatcg tgtagtcgtc gcccatgatc catctgcgcc ccgacagccg tccttccagc
     2041 accgcgagca agcgttttac ttcggcaaca taacggtcgc gtggacgctt gtcttcgtag
     2101 tctttgccgg cgaatttatt gaaaaaacca agctggccga acatcgggcc gatgcccccc
     2161 atctggaaca tgagccattg gatggtctcg taacggccgg cggcgtcgcg cggcatgaac
     2221 tgcctcgatt tatcagacag gtagatgagg attgcgcccg attcgaacaa cggaagcggt
     2281 ttgccgtccg ggccgtcggg atcaagaatg gccggaatct tgttgtttgg gctaagcgat
     2341 agaaattccg gcgacttctg gtcgtgggtc tggaagctga ctagatgagg ctcatagggt
     2401 aggccggtct cttcgagcat gaccgagacc ttgatgccat tgggagtcgg caaggaataa
     2461 agctggatgc ggtcaggatg ctgcgggggc catttcttgg tgacaaggaa agtggaaagg
     2521 tcagtcatag tggtggttct gtaagattga aggcgcacgg aactacttat ataccgcagg
     2581 gtcgggcgag tcggtggggt gctcgaatgc tttcttaagc tcgggcgata taggccaatt
     2641 cagattgacg tttgtgggcg gtatgggccg ggtgaaccat ttgttgtaca aagcattgat
     2701 tgcgccgttc gccataatcg cttttaccgc gttgtcaacc gccgttttaa acggttcatc
     2761 ggctttgggt tccatgatgc cataaggttc gatggtgtag gccgtatcgc tgatcatcca
     2821 gtcattcggg ctatgtgaat tcgccaccag tccggccagc aaaacgtcat ccatgaaaaa
     2881 tgcggatgcc cggcctgtgt caacgctaaa gaggccttca ggctgaccct tggcggtaat
     2941 aattttcatg ccaagttgct gctcgtcgtt ggtcttggtc agccaccgca agttggagct
     3001 gcccgagacg cctacaacaa ctttgccgtt gaaatcatga agatctttca agtgcgattt
     3061 tttcagcgcg gcaaagcgtg ttgctgtaac gaatgtggtt ggcgcaaaac taacttgtgt
     3121 ctggcgctgc gcgttgttgg tggacacgcc gcacacgagg tcgaccgtcc cgttggctat
     3181 tagcggaatt cgcgacgcgg gcgttaccgg caccatcttg gtgtggagat cgggtagatt
     3241 aaggtttttc tttagtgtgt cgacaatgct gtggcaaatg tctattg
//
LOCUS       DATCXU010000131         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_249690,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000131 DATCXU010000000
VERSION     DATCXU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249690"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="ugpC"
                     /locus_tag="VIR04_02910"
     CDS             complement(<1..770)
                     /gene="ugpC"
                     /locus_tag="VIR04_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY9459732.1"
                     /translation="MASVEIKNVTKSFGAQQVLHGVDLDIHDGEFVALVGPSGCGKST
                     LLRMIAGLEDISGGEIVIGGVTVNHLAPKDRDIAMVFQNYALYPHMTVRENMAFSLKR
                     RKTPMDEIHRRVSLAADILGMGPYLDRHPRQLSGGQRQRVAMGRAIVRNPNVFLFDEP
                     LSNLDAKLRVNMRAEIKALHQRLRTTSVYVTHDQVEAMTMADRIVVLSGGRVEQVGTP
                     LSLYDRPANRFVAGFIGSPAMNFIPGVLRNDRDGASVHA"
     gene            complement(780..1589)
                     /locus_tag="VIR04_02915"
     CDS             complement(780..1589)
                     /locus_tag="VIR04_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY9459733.1"
                     /translation="MFRDNASWWMYAVAILAGLGAVLPLAWLFMLSLDPSQGAGTSGI
                     AYSFQNYSSVLFESLMGRYFLNTGLVALATVVCTLTCACMGAYAASRYRFRGREVAMV
                     GLLIASMTPVIAVLVPLYAYAAKLQMLNTYQVLIVIFTAWQLPGTLWLIRSFMDAIPV
                     ELEEAALVDGCSRSGAFFRVVLPQLAPGLVAAGLIVFVYVWNEFIVAVSLAPKQDMRL
                     ISVGLYFYLSDYGVEWGKISAGAILSILPPAVLFLFLQRQLIHGLSAGAVK"
     gene            complement(1576..2463)
                     /locus_tag="VIR04_02920"
     CDS             complement(1576..2463)
                     /locus_tag="VIR04_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY9459734.1"
                     /translation="MAVSTTSFRQASPYLLLVPAAVFVLVVTVYPIISLFYTAFHSTN
                     YFQIGAFNGFANFASLFSSSGAKSFTASAVFVVASDIITVALALFLALILEAPLRKRG
                     VLRTFIMLPWLVSMVVTSLLWQAMLDPNFGPITQLVYSVFGWHISLLSDGTSAMAVLV
                     LANVWRSYPFALVLILAALQSIPGELYEAARLDGASRWDELRHIALPIAGPTITVVLI
                     LLTFEYFTLVTLPFIMTSGGPNEATYLLSLRIWREAFTNYQFGASAATGVVVFLLNLL
                     LSAFYIYHFVLKRTNDVSR"
     gene            complement(2466..>3356)
                     /locus_tag="VIR04_02925"
     CDS             complement(2466..>3356)
                     /locus_tag="VIR04_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9459735.1"
                     /translation="DFLMPLENTIYDGQTMAYYWSTLLGNEFWYLKDVANGAPPLKWN
                     DLPAYLKAAGDKAGVPGFIIGLSQQGNGIGITNPLIPALWACGADYVTPSGEIGFANA
                     NGEKAFQWLLDLVRVHKVTPESIVSLTRDNVLDAIKGRKAVSVILSSNVVSSARASLG
                     DDMGLARQPGPNGPCPAFATGKFLIMTKSCKEKEATGLFIRSMISADAQLANAKIGSE
                     IPVLKSVAHDPYFSTPEAADIKFALDYMASNPHPFKYPMRTDYLQTRIALATQQMFKG
                     TPIKDAIEQVANDWNTARKG"
BASE COUNT          862 a          978 c          896 g          620 t
ORIGIN      
        1 gcatgcaccg acgcgccgtc ccgatcgttg cgcagtactc ccggaataaa attcatcgct
       61 ggcgagccga tgaatccggc gacaaatcgg ttggccggcc gatcgtaaag ggaaagcggc
      121 gtacccacct gttccacacg gccgccactt agcacaacga tccgatcggc catggtcatc
      181 gcctcgacct ggtcatgcgt gacatacacc gacgtcgtgc gcaagcgctg atgcagggcc
      241 ttgatctcgg cgcgcatgtt cacgcgcaac ttggcatcca gattggacag cggctcatca
      301 aacaggaata cgttgggatt gcgcacaatg gcgcgcccca tggcaacacg ctggcgctgc
      361 ccgccggaca actgacgcgg atgacgatcc agatagggcc ccatccctaa aatatcggcc
      421 gccaaactga cgcgccgatg gatctcgtcc ataggggttt tgcgccgttt aaggctaaac
      481 gccatatttt cacgaacggt catgtgcgga taaagcgcat agttctggaa caccatggcg
      541 atgtcccggt ctttgggcgc aaggtgatta acggtcacac cgcctatgac aatctcgcct
      601 ccgctaatat cttccaggcc ggcaatcatg cgcaaaagcg ttgacttgcc gcaacccgag
      661 gggcctacca gggcaacaaa ctcgccatcg tggatatcaa ggtcgacgcc atgcaatact
      721 tgctgcgcgc caaaagactt ggtcacattt ttgatttcga ctgaagccac aacgttcccc
      781 tacttgaccg cgccggcgct aagtccgtgg atcagttgcc gttgcagaaa caggaacagc
      841 accgccggag gcaggattga aaggatggcg cctgcggaaa ttttgcccca ctccacccca
      901 taatccgaca gataaaaata caggcccacc gagatcaacc gcatgtcctg tttcggcgca
      961 aggctgaccg caacgataaa ttcgttccag acataaacga agacgatcag accggccgca
     1021 accaggccag gcgccagttg cggcagcacg acgcgaaaaa atgcgcccga acgcgaacac
     1081 ccgtcgacca gcgcggcctc ttccagttcg accgggatcg cgtccatgaa tgaccggatc
     1141 aaccacaggg tgccaggcaa ttgccacgcc gtgaaaatca caatcaggac ctgataagta
     1201 tttagcattt gcaactttgc cgcatacgca taaaggggta ccaggaccgc aatcaccggc
     1261 gtcatcgacg cgatcagtaa ccccaccatc gccacctcgc ggccgcgaaa gcgatatctt
     1321 gaggccgcat aagcccccat gcaggcgcac gtcagggtac agaccaccgt tgcaagcgca
     1381 acgagaccgg tgttgaggaa atagcggccc atcagagact cgaacaacac tgatgaatag
     1441 ttttgaaacg aatacgcgat gccggaggtg ccggccccct gggaagggtc aagcgacaac
     1501 atgaaaagcc atgccagcgg caaaacagcg cccaaacccg ctagtatcgc gaccgcatac
     1561 atccaccagg aagcgttatc gcgaaacatc gttcgtcctt ttcaacacaa agtgatagat
     1621 atagaaagcg ctcaacagca aattaagcag gaaaacaacc acgccagtcg ccgcggaagc
     1681 gccaaactgg taattggtga acgcctcgcg ccatatgcgc agcgacaaca ggtaggtggc
     1741 ttcgttcggc ccgccactgg tcatgatgaa aggcaaggtc accagggtga agtattcgaa
     1801 tgtcaaaagg atgagcacaa ccgtgatggt cgggccggca atcggtagtg cgatgtgacg
     1861 caactcgtcc cagcgcgacg caccatcaag ccgcgccgcc tcgtaaagtt cgccaggaat
     1921 ggattggagc gcagccagaa tcagcacgag cgcaaacgga taggaacgcc agacgttggc
     1981 caggaccagg acggccatgg cggaagtccc gtcgctgagc aatgaaatgt gccacccgaa
     2041 taccgaatat acgagctgtg tgatcggtcc gaaattcgga tcaagcatcg cctgccacag
     2101 caaggacgtg accaccattg agacgagcca cggcaacatg atgaacgtgc gcagtacacc
     2161 acgcttgcgc aatggcgctt ccagtatcaa tgccagaaac agcgccagcg ccaccgtaat
     2221 aatatcggac gcgaccacga ataccgccga ggccgtgaac gactttgcac cactggagct
     2281 gaacagcgat gcaaagttgg caaaaccatt aaacgcgcct atttgaaaat aattcgtcga
     2341 atggaatgcg gtgtagaaca ggctgatgat cggatacacc gtaaccacca gcacgaacac
     2401 cgcggctgga accagcaata gataagggct tgcctgccgg aaactcgttg tcgatacggc
     2461 cacggtcaac ccttgcgcgc ggtgttccag tcgttggcca cctgctcaat ggcatctttg
     2521 attggcgtgc ccttgaacat ttgctgcgtc gccaatgcaa tgcgagtttg caaataatcg
     2581 gtacgcatcg gatatttgaa cggatgcgga ttggacgcca tgtaatccag cgcgaatttt
     2641 atgtcggccg cttcgggcgt agaaaaatac ggatcgtggg caacagactt caatacgggg
     2701 atttcagagc caatcttggc gttcgccagt tgcgcgtcgg ccgatatcat cgaccgaata
     2761 aacaatccgg tggcctcttt ctctttgcat gacttggtca taatcaggaa tttcccggtt
     2821 gcaaacgcgg ggcacgggcc attcgggccg ggttgacgcg ccaaccccat gtcatcgccc
     2881 aacgatgcgc gtgcggagga aacgacattg ctgcttaaga tcacagagac ggccttgcgc
     2941 ccttttatgg catcaagcac gttgtcacgc gtaagcgaaa cgatggattc aggagtgacc
     3001 ttgtgcacac gcaccaaatc caggagccac tgaaatgcct tttccccgtt ggcattggca
     3061 aaaccgattt cgccgctggg cgttacgtag tccgcgccgc atgcccacaa cgccggaatt
     3121 aacgggttgg taatgccaat gccgttacct tgctggctta atccaatgat gaagccaggg
     3181 acgccggcct tatcgccggc agccttcaag tatgcaggca agtcattcca ctttaacggc
     3241 ggcgccccat tggcgacgtc cttgagatac caaaactcgt ttccgagcaa cgtgctccag
     3301 taatacgcca tggtctgacc gtcatagatg gtgttttcca aaggcatcag gaaatc
//
LOCUS       DATCXU010000132         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_559339,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000132 DATCXU010000000
VERSION     DATCXU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559339"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="pyk"
                     /locus_tag="VIR04_02930"
     CDS             complement(<1..1060)
                     /gene="pyk"
                     /locus_tag="VIR04_02930"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY9459736.1"
                     /translation="MPARRTKIVATLGPTSSSPEKIEQLILAGMNVARLNFSHGEADD
                     HRQRVKHIRAMAAKHKKYIAIMGDLQGPKIRIARFKNSKVTLAVGQTFTLSNKHPKDA
                     GDETIVGIDYPPLVHDCKAGDELLLDDGRVVLSVRSVDEHAVYTVVTQGGPLSNNKGI
                     NRRGGGISAPSLTKKDKTDILLAAELELDYVAVSFPRYGRDIEQARKLVRAAGSSAWL
                     IAKIERAEAVVDNEALDALIEISDGVMVARGDLGVEIGDARLAGIQKRIIQRARSHNK
                     LVITATQMMESMISSPMPTRAEVSDVANAVLDYTDAVMLSAESASGQYPVEAVAAMAR
                     ICLGAEQEPMTTQSKHRLG"
     gene            complement(1092..1562)
                     /gene="rlmH"
                     /locus_tag="VIR04_02935"
     CDS             complement(1092..1562)
                     /gene="rlmH"
                     /locus_tag="VIR04_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY9459737.1"
                     /translation="MKLLVIAVGVRMPDWVETAWREYAKRLPSDCALELREIKPEPRT
                     SGKTPAQMKLAEARRIEASIPAHARKIALDEHGRDMTTIDLTHELQAWRANGQDIAIL
                     VGGPDGLDDQLKESCHGRWRLSSLTLPHAMVRVIVAEQLYRAWAIMTRHPYHRA"
     gene            complement(1565..1960)
                     /gene="rsfS"
                     /locus_tag="VIR04_02940"
     CDS             complement(1565..1960)
                     /gene="rsfS"
                     /locus_tag="VIR04_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY9459738.1"
                     /translation="MDIKKLQRIVIDALEDVKAQDIKIFNTSHITSLFDRVVIASGAS
                     NRQTRALASSVADRARELKIPIIAYEGSETGEWVLVDLGDIVIHCMQPAIRRYYDLEG
                     IWGGRPARVKLLPQSTQPSTGEPYGFDEQ"
     gene            complement(1983..>2463)
                     /locus_tag="VIR04_02945"
     CDS             complement(1983..>2463)
                     /locus_tag="VIR04_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEY9459739.1"
                     /translation="NPWQRKPLAATAAQRLAMLELAIAGENCLRINPIEIKRGGETRT
                     IDTINSLPENIDYTWILGADQLANFCSWHCWQDIAARVKLAVAQRPGTPLAPPPPLMR
                     HLAALNRTLIELPFEPVSISASAIRERLAQGLPTQGLLHVAVAQYIEQNKLYQTPYS"
BASE COUNT          443 a          690 c          738 g          592 t
ORIGIN      
        1 ctcccaggcg gtgcttggat tgcgtcgtca tgggctcctg ttcggctcca aggcaaatgc
       61 gcgccattgc cgcaaccgcc tcgaccggat actggcccga cgcactttcg gccgacagca
      121 tgacggcgtc ggtatagtcc agtacggcat tggccacgtc ggacacctcg gcgcgggtgg
      181 gcatcgggct ggaaatcatc gattccatca tttgcgttgc cgtgatgacc agcttgttgt
      241 gcgaacgggc gcgctgaatg attcttttct ggattcccgc caggcgcgcg tcgccgattt
      301 caacgcccag atcaccgcgc gccaccatga ccccgtcgct gatttcgatc agtgcgtcga
      361 gcgcttcgtt gtcgaccacc gcttcggcgc gttctatctt ggctatcagc caggcgctgc
      421 tgcctgccgc gcgaaccagc ttgcgggctt gttcgatatc gcggccatag cggggaaagg
      481 aaacggcgac ataatccagc tcgagctccg ccgccagcag gatgtcggtc ttgtctttct
      541 tggtcaggct gggcgccgag atgccgccgc cccggcggtt gatccccttg ttattcgaaa
      601 gagggccgcc ctgtgtcacc actgtgtaga cggcatgttc gtccacgctt cttacactca
      661 gcacaacacg cccgtcgtcc agaaggagtt cgtcgcccgc cttgcaatcg tgcacgaggg
      721 gagggtagtc gatacccact atcgtttcgt cgccggcgtc tttcgggtgc ttattcgata
      781 gcgtgaaagt ttgcccgacg gcgagcgtga ccttcgagtt tttgaagcgg gcaatgcgaa
      841 tcttgggacc ctgcagatcg cccattatcg cgatatattt tttgtgcttt gcagccatgg
      901 cgcgtatgtg ttttacgcgt tggcggtggt cgtcggcctc gccgtgggaa aaattcagcc
      961 tggcgacatt cattcccgcc agaatgagtt gctcgatctt ttccgggctg gagctggtgg
     1021 ggcccagggt agctacgatt ttcgtgcggc gtgctggcat tattgaacca ttgtgtgttt
     1081 attcaagcct ttcaggctcg gtggtaaggg tgtcgggtca tgatagccca ggcgcggtat
     1141 agttgctcgg ccacgatcac gcgcaccatg gcgtgcggca gagttaaaga tgataatcgc
     1201 caccggccat ggcagctttc cttcagttga tcgtccagtc cgtcgggacc acccaccagg
     1261 atggcgatgt cctgtccatt ggcgcgccac gcttgcagct cgtgagtcaa atcgatggtg
     1321 gtcatgtccc ggccatgctc gtccagagca attttgcgtg cgtgtgccgg aatggatgct
     1381 tcaatgcgcc gggcctcggc cagtttcatt tgtgccggcg ttttacctga tgtacgcggc
     1441 tcgggtttga tttccctgag ttccagggcg cagtcggaag gaaggcgttt ggcatattcg
     1501 cgccaggctg tttcgaccca gtccggcatg cgcactccga cggcgatgac aagaagcttc
     1561 actgctattg ttcgtcgaac ccatagggtt cgcctgtgct gggttgagtc gactgcggca
     1621 agagcttgac acgtgccggc ctgccgcccc agattccttc gagatcgtag tagcgccgta
     1681 ttgccggctg catgcaatga atgacgatat cgcccaagtc gaccagaacc cattcgcccg
     1741 tttcagagcc ttcataggca atgatcggga tcttgagttc gcgcgcccga tcggcgacgc
     1801 ttgacgccag cgcccgggtc tggcgatttg acgccccgct ggcaatgacc acccgatcga
     1861 acaggctggt tatgtgcgag gtattgaaaa ttttgatgtc ttgcgcttta acgtcttcaa
     1921 gggcgtcaat gacgatccgt tgcagttttt ttatatccat gcccgaacta taacgtgaga
     1981 cgctatgaat aaggagtctg atacagcttg ttttgttcga tatattgcgc gacagcaaca
     2041 tgaagcaggc cttgcgtggg caaaccttgc gccaggcgct cgcggatggc ggaggccgaa
     2101 atagatactg gttcgaatgg caattcgatc aatgtgcgat tcagcgcagc cagatgccgc
     2161 atcaatggcg gcgggggggc caacggggtt ccggggcgct gggcaacggc taattttacg
     2221 cgggcggcaa tgtcttgcca gcaatgccat gagcaaaaat tggcaagctg atcggcgccc
     2281 aatatccagg tgtagtcaat gttttccggc aggctgttta tcgtatcgat tgtgcgggtc
     2341 tccccgcctc gttttatctc gatagggttg atgcgcaagc aattttctcc cgctatcgca
     2401 agctccagca ttgcaaggcg ctgtgcggct gtggcggcta acggcttgcg ctgccacgga
     2461 ttg
//
LOCUS       DATCXU010000133         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_267428,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000133 DATCXU010000000
VERSION     DATCXU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267428"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VIR04_02950"
     CDS             <1..592
                     /locus_tag="VIR04_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY9459740.1"
                     /translation="SHSALAYLGGLAGHATVAAAIRDEVLASFIDGLMDDAAQTLDMP
                     AGVDLGDYRRSLLQRFRNVSLRHLTAQIAMDGSQKLPQRILAPIRERLAQGLDIDRHA
                     MVVAAWIRYLQGRDEHGAVLTVDDPMAAELSAIVKSTEGSAQELAQAVLGVSAIFGRD
                     LPMNPAFVLGVTQALQRLLCDGVGAALSTPNFARVQ"
     gene            592..1773
                     /gene="uxuA"
                     /locus_tag="VIR04_02955"
     CDS             592..1773
                     /gene="uxuA"
                     /locus_tag="VIR04_02955"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEY9459741.1"
                     /translation="MEQTWRWFGPRDPIPLNHIRQAGATGIVTALHHIPAGVAWPEAD
                     IARRQELIRAAGLRWSVVESIPVPESIKLRRGNFESDIQAWKTSLVRCAEAGIRTVCY
                     NFMPVVDWTRTDLMWQRPSTAYALRFDINDFAVYDIFILQRENARDSFEDQVVQEAER
                     RFARLSPADIERLEKTIIAGLPGADVSYDRASLKRLIAQYDEVGPEDLARNLTLFLEE
                     VAPVAQAQGVHLGIHPDDPPMSLFGLPRVVSTRTDLQRLLDAVPMPANGLTLCVGSLG
                     VRADNRLVEMVRAFADRIHFAHLRDVTYEANGSFFEAEHLDGSSDMIGVVASLLDEER
                     RRHDQGHSQLEIPMRPDHGQLLGDDINKPTNPGYSYIGRLKGLAELRGVIRTLQYAGP
                     R"
     gene            1804..2271
                     /locus_tag="VIR04_02960"
     CDS             1804..2271
                     /locus_tag="VIR04_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY9459742.1"
                     /translation="MTEVETPKDMAAYVGKLLGVSDWVTVDQDMIDQFAHVTGDNNWI
                     HVDVERAKHELPDGKTIAHGMLTMSLMTGLGLRTYNIRQRGKSINYGSNKVRFMSPVP
                     SGTRIRLHRTLEKCEPADGAVRLTFSNIVEIEGGKRPAMVAESISLVYEKGAA"
     gene            2406..2831
                     /locus_tag="VIR04_02965"
     CDS             2406..2831
                     /locus_tag="VIR04_02965"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY9459743.1"
                     /translation="MSKKASAVWKGGIKDGGGTISTETGALKEAPYGFKSRFENGAGT
                     NPEELIAAAHAGCFSMAFSLMLGEAGFTPEKIETQAAVTLVSNADGFKVTSSHLTVTA
                     KIPGIDNAKFKQIAEQAKAGCPISKLLNTTITMDATLKA"
     gene            complement(2925..4244)
                     /locus_tag="VIR04_02970"
     CDS             complement(2925..4244)
                     /locus_tag="VIR04_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY9459744.1"
                     /translation="MNLWIDKDKAGSTMSESTTRAIHEALEGRRTGVSMLLPFAGPAV
                     VVSVAYMDPGNFATNIQAGARYGYTLLWVVLMANVIAILFQGLSAKVGIVTGRNLAEL
                     CRERFPKPLVLLMWAVSEIAAMATDLAEFLGGAIGLSLLFDMPLLLGMAVTAIVTYGL
                     LMFDRNGFRPLELTIGALVGIIGLSYLAELFIAPVQWPGVFQHMFTPDLPDAYALTIA
                     VGIIGATVMPHALFLHSGLTQHRSPARSEKERAKLLRYSNIEVVIALTIAGLINMAMV
                     IMAAGAFHAGHADVAEIETAYHTLAPLLGISAAGIFLLSLIASGVSSSVVGTMAGQMI
                     MQGFVGFKIPLWLRRAITMVPSFVVVGLGVNATQALVLSQVVLSLALPFPMVALVWFT
                     CRRDIMGAYKNRALIVVVSTIAAAAVITLNAVLLLQTAGVAIPGLPG"
     gene            4360..>4885
                     /locus_tag="VIR04_02975"
     CDS             4360..>4885
                     /locus_tag="VIR04_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY9459745.1"
                     /translation="MIKIWGRENSVNVKKALWCAQELGLAYERVNAGGAFGVVDTPEY
                     RAMNPNGLVPCIEEDGFILWESNAIVRYWCAKYGVGTLFPADLRKQAIAGQWMDWSTS
                     IFTPLYRDVMRNLIRLPEQQRNMSVVEKSVRDGNQHLATLDAVLAKQPFLSGDAFGMG
                     DIPMGCLAYGWLKMP"
BASE COUNT         1159 a         1431 c         1361 g          934 t
ORIGIN      
        1 cagccattcc gccttggcgt atctcggggg acttgcgggc cacgcaaccg tggctgcggc
       61 cattcgcgac gaggtactgg cgagtttcat cgacggttta atggacgatg ccgcgcaaac
      121 gctggacatg ccggcgggcg tcgatctggg cgattaccgg agatcgctgc tgcaacgctt
      181 tcgcaatgtg tcgctcaggc atcttaccgc gcaaatcgca atggacggtt cgcaaaaatt
      241 gccccagcgc attttggcgc ccattcggga acggctggcg caaggccttg acatagaccg
      301 ccacgctatg gtcgtggccg cctggatacg ttatttacaa ggccgcgacg agcacggcgc
      361 cgtattgacg gtcgacgatc cgatggcggc cgaattaagc gcgatcgtta aaagtaccga
      421 aggatcggcc caggaactgg cccaggcagt ccttggcgta agcgccatat tcggacggga
      481 cctgcccatg aatcccgctt ttgttcttgg cgtgacacag gcactgcagc gattattatg
      541 cgacggtgtg ggcgcggcct tatccacgcc aaattttgcg agagtccaat aatggaacag
      601 acgtggcgct ggttcggccc ccgggatccc atccccctga accatatccg tcaggcggga
      661 gcaaccggca ttgtcacggc actgcatcat atccccgccg gcgtggcctg gcccgaggcc
      721 gacatcgctc gccgccagga gcttatccga gcggcaggcc tgcgctggtc ggtggtcgaa
      781 agcataccgg tacccgagtc gatcaagctg cgtcgcggca acttcgaatc cgatatccag
      841 gcgtggaaga cgtcactcgt gcgctgcgct gaagcgggca tacgcacagt ctgctacaac
      901 tttatgcccg ttgtggactg gacccgcact gacttgatgt ggcaacggcc gtcaacggcg
      961 tatgccttgc gattcgatat caatgatttc gcagtctacg atatttttat attgcaacgc
     1021 gaaaatgcgc gcgatagctt cgaggatcaa gtcgtgcagg aagccgagcg acggtttgcg
     1081 cgtttaagtc cggccgacat cgagcgcctg gaaaaaacca tcatcgccgg cttgccgggc
     1141 gcggatgtgt cctatgaccg ggccagcctg aaacgcctga tcgcccagta tgatgaggtc
     1201 ggccccgaag atctggcgcg caacctgacc ttgtttctcg aggaagtcgc gccagttgcg
     1261 caggctcagg gcgtgcattt gggaatccat ccggatgacc ctcccatgtc tttattcggc
     1321 ttgccgcgcg tagtgtccac cagaaccgat ttgcaacggc tgctggatgc agtgccaatg
     1381 cctgccaacg gactaacgct gtgtgtgggt tcgttgggcg tacgcgcgga caaccggctc
     1441 gtcgagatgg tgcgcgcttt cgccgaccgc attcacttcg ctcatttgcg cgacgtcaca
     1501 tacgaggcga atggctcgtt tttcgaggcc gaacacctgg acggttcatc cgatatgatc
     1561 ggtgtggtcg ccagcctgct cgatgaggag cggcggcggc acgaccaggg ccacagccaa
     1621 ctggaaatcc cgatgcgtcc ggatcacggc cagttgctgg gcgacgacat caacaagccg
     1681 accaatccag gctattcata tatcggacgc ctgaagggtc tggcggaatt gcgtggtgtc
     1741 attcgaacgc tgcaatacgc gggcccgcga tagcggctac ctcaaaatca aggaaagaac
     1801 atcatgactg aagtcgaaac tccgaaagac atggccgctt atgtgggcaa actgctgggc
     1861 gtcagtgact gggtcaccgt cgaccaggac atgatcgacc agttcgcaca cgttaccggc
     1921 gacaacaact ggattcacgt cgatgtcgaa cgcgcaaagc atgaactgcc cgatggaaaa
     1981 acaattgccc acggcatgct gaccatgtcg ctcatgacgg gactgggctt gcgcacctac
     2041 aacatccggc agcgcggcaa gagcatcaac tatggctcaa acaaggtgcg ctttatgagc
     2101 ccggtcccgt cggggacgag aatccgcctg catcgaaccc tggaaaagtg cgaaccggcc
     2161 gacggtgcag tacgtctgac cttttccaac atcgtcgaaa tcgaaggcgg aaaacgcccc
     2221 gccatggtgg cggagagtat ttcactggtt tatgaaaagg gcgcggcatg aacgtagaaa
     2281 tccatagcgc cgctcggtag ccatcagcga actgaatacc cacactgcgg atgtcggatc
     2341 agtgccggtg ttaacatcaa gcatgcatca ttgcagcgaa gcaatccaat aatcaaggag
     2401 tcgaaatgag caagaaagca tccgcagtgt ggaaaggcgg gatcaaagat ggcggcggca
     2461 ctatttccac cgaaaccggc gcgctgaaag aagcgcctta tggattcaaa tcgcggtttg
     2521 aaaacggcgc gggaacgaac ccggaagaac tgatcgccgc agcgcacgcc ggctgcttct
     2581 caatggcgtt ttcattaatg ctcggcgaag caggcttcac gcctgaaaaa atcgaaacac
     2641 aggccgcagt cacccttgta tcgaacgccg atggattcaa ggttacatcc agccatttga
     2701 cggtaaccgc caagatcccc ggtatcgaca acgccaaatt caagcagatt gccgaacaag
     2761 ccaaggccgg ttgccccatc tccaaactgc tgaacaccac catcacgatg gacgccacgc
     2821 ttaaagcctg aaccagcgct gaaaaccgtt cgctttaacc aggcgcaacg caaagggcgg
     2881 gcaaacgccg gccctttgcg ttattgcgta aacagccgga tggcttaccc tggcaaacca
     2941 ggaatggcaa cgcccgccgt ctgaagcaaa aggacggcat tcagcgttat cacggcggcg
     3001 gccgcaatgg tggagacgac cacaatcaag gcgcgatttt tatacgcccc cataatatcg
     3061 cgccggcacg taaaccatac caaagccacc atgggaaacg gcaacgcaag gctaagcacg
     3121 acctggctta atacaagcgc ctgggttgca ttcaccccca atcccacaac gacgaagctg
     3181 ggcaccatgg ttattgctct tcgcagccat aacggaatct tgaacccgac aaagccctgc
     3241 atgatcatct ggccggccat tgtgcccaca acggagctgg aaaccccgga tgcgatcaac
     3301 gacagcagaa aaattcccgc cgcgctaatg ccaagaagcg gtgcgagcgt gtgataggca
     3361 gtttcgattt cggcgacatc cgcatgccct gcgtgaaacg ctcccgccgc catgatgacc
     3421 atcgccatgt ttatcaggcc cgcaatcgtc aacgcgatca ccacctcgat gttggagtat
     3481 ctgagcagct tcgcccgttc cttctcggag cgcgccggag atcgatgttg ggtcaggccc
     3541 gaatgcagaa aaagcgcgtg cggcataacg gttgcgccaa tgattccaac cgcgatggtc
     3601 agggcatacg catcgggcag atctggcgta aacatgtgct gaaaaacgcc cggccactga
     3661 accggagcaa tgaataattc ggccaggtag gacaggccaa taatgccgac cagcgcgcct
     3721 atggtgagct cgagcggacg aaagccattg cgatcgaaca tcaacaagcc ataagtcacg
     3781 atcgctgtca cggccatgcc gagcaataac ggcatgtcaa acaacaaaga caatccgatc
     3841 gcgccaccga gaaattcggc caggtccgtc gccattgcgg cgatctcgct taccgcccac
     3901 atcagcagca cgagcggttt tgggaaacgc tcacggcaca gttcagccag attgcggccg
     3961 gtcacaatac cgactttcgc cgaaaggccc tggaacaaaa tggcgataac attggccatc
     4021 aatacgaccc acaacaatgt gtagccatat cgggcgcccg cctgaatgtt ggtcgcaaaa
     4081 ttgcccgggt ccatataagc aacggacacc acgaccgccg ggccggcaaa cggaagcagc
     4141 atcgagacac ctgttcgcct tccttccagc gcttcgtgaa tcgcgcgagt cgtgctttcg
     4201 ctcattgtcg agcctgcttt gtccttgtcg atccacagat tcatcgtccg cgcttccctc
     4261 ttccactacc catcaaaaat cacgctcatg agctagggca attctactcc ggggccacac
     4321 cccatataat cagtgcaccc actatgaaaa ggagacgtca tgataaaaat ctggggccgc
     4381 gaaaattccg ttaacgtcaa aaaagcgctc tggtgcgcgc aggagctcgg gcttgcatac
     4441 gaacgtgtaa acgccggagg cgctttcggg gtggtggata cgcccgagta tcgggccatg
     4501 aatcccaacg ggctcgtgcc ctgcattgaa gaagacgggt ttattctttg ggagtccaac
     4561 gccattgttc gctattggtg cgccaaatat ggcgtcggca ctctgtttcc agccgacctg
     4621 cgaaagcagg ccattgcagg ccaatggatg gactggtcca cgtcaatttt cacgccgctt
     4681 tatcgggatg taatgcgcaa cctgattcgt cttcctgagc aacaaaggaa tatgtctgtc
     4741 gtcgaaaaaa gtgtgcggga tggcaaccag catctcgcaa ccctcgacgc cgttctggcc
     4801 aaacagccct tcctgtcggg agacgcattt ggcatgggcg acattcccat ggggtgcctg
     4861 gcttatggct ggctcaaaat gccca
//
LOCUS       DATCXU010000134         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1131831,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000134 DATCXU010000000
VERSION     DATCXU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131831"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VIR04_02980"
     CDS             complement(<1..1510)
                     /locus_tag="VIR04_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY9459746.1"
                     /translation="MSASFPSGWPFPVSVAPDKLASIQAQFAGDWMRMASSAGQGTLD
                     RPADRRFSGPAWSEDPSSLIMAHTYLLSVQALNQMVEAAQVTESVRNRLRFSVMQWAD
                     AMSPSNFFATNPDAQRALMQSGGDSLRLGIGNLLADLSKGRMSQTDESGFQLGKNIGV
                     TEGAVVFENAYMQLIQYAPQTPKVYKKPLLIVPPCINKFYILDLQPENSFVRHAVEHG
                     FTVFMISWRNPLASDVDGIDTAGWTDYLQDGVLKALDIASDISGQEQVNALGFCVGGT
                     LLASALTLAQARGKNPVSSLTLLTTLLDYHDTGILDVFVDEMQVQAREFQLGQGGLMS
                     ARELATTFSFLRPNELVWNYVVNNYLKGLTPASFDLLFWNADGTNLPGPFFTWYFRNT
                     YLEDNLKLPGRIKIDGHALDLRLLTMPAYVYGSREDHIVPWQSAYASTGILRGPLRFV
                     LGASGHIAGVINPPAKKRRHYWAHEMTYTHADIPGDPQSWLDDAVQHPGSWWP"
     gene            complement(1606..>2252)
                     /gene="pgeF"
                     /locus_tag="VIR04_02985"
     CDS             complement(1606..>2252)
                     /gene="pgeF"
                     /locus_tag="VIR04_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY9459747.1"
                     /translation="PSCVVENRSRLGDRLPGEPLWLNQVHGASVFDADVDNGAIGGPP
                     VADAAVTTRVDRVLAIMTADCLPIVIAATDGLVLGVAHAGWRGLAGGVLEQTLARLRA
                     AHPHPAHAQWHAWVGPAISQRHFEVGADVFSAFVDRDGLSRVFFAEKIPNRKWLADLP
                     GLARHRLYQLGVADIELSGYCTYGQPDFFYSYRRDPLCGRMATIAWLSGVDADK"
BASE COUNT          541 a          650 c          619 g          442 t
ORIGIN      
        1 ccggccacca gctacctggg tgctgcaccg cgtcgtcgag ccatgactgc gggtcgcccg
       61 gaatgtctgc gtgcgtatag gtcatttcat gcgcccagta gtgacggcgt tttttggcgg
      121 gaggattaat gacgcccgcg atatgccccg aagcccccag cacgaaccgc aacggcccgc
      181 gcaggatgcc cgtcgaagca taggcggact gccacggcac gatatgatcc tcgcgcgagc
      241 cgtaaacata ggctggcatg gttaataaac gcaaatcaag tgcgtgcccg tctattttga
      301 tgcgccccgg gagtttcaaa ttatcttcaa ggtaggtatt gcgaaagtac caggtgaaaa
      361 acggccctgg caaattggtg ccatcggcat tccagaacag caaatcaaat gacgcgggcg
      421 tcaaaccttt aagataattg ttgaccacgt aattccagac gagctcgttg ggtcgcagaa
      481 acgagaatgt ggtcgccagt tcgcgcgccg acatgagccc gccttgcccc aactggaatt
      541 cgcgggcctg tacttgcatt tcgtcaacaa agacatcaag tatgcccgta tcgtgatagt
      601 cgagcagcgt ggtcagtaat gttaacgaac tgacagggtt ttttccgcgc gcttgcgcca
      661 atgtcaatgc gctggcaagc aaggtgccac ctacgcagaa gcctagggca ttgacctgtt
      721 cttgtcctga aatgtcgctt gcgatatcga gcgccttaag cacgccatct tgcaaatagt
      781 cggtccagcc cgccgtgtca atgccgtcga catcggacgc cagcggattg cgccacgaga
      841 tcatgaaaac ggtgaaaccg tgctcaacgg cgtgccgcac aaacgaattt tcgggttgaa
      901 gatccagaat ataaaacttg ttaatgcacg gcggcacgat caatagcggc tttttataca
      961 ctttcggggt ttgcggcgcg tactgaatca actgcatata ggcgttttcg aacaccacag
     1021 cgccttcggt gacaccaata ttttttccga gctgaaagcc tgattcatcg gtttgggaca
     1081 ttcgtccttt gctcaggtct gcgagcaaat tgccgatacc cagacgcaac gagtcaccac
     1141 cgctttgcat cagggcgcgc tgggcgtcgg gattcgttgc aaagaaattg gacggcgaca
     1201 tcgcgtcggc ccattgcatg accgaaaacc gcagccggtt gcgcacggat tcagtcacct
     1261 gtgcagcttc gaccatttgg ttcaaggctt gaacagacaa taaataagtg tgggccatga
     1321 tcaaagaact gggatcttcg gaccatgccg gaccgctgaa acgccgatcg gcgggcctgt
     1381 caagcgttcc ctgcccggcg ctggacgcca tgcgcatcca gtcgcctgca aactgagcct
     1441 gaatgctggc cagcttgtct ggcgccacgc taaccggaaa gggccacccg gatggaaacg
     1501 aagcactcat tcgaacacct gcatataaag actgaaaccg taggtcaatg ctggatggca
     1561 atgaaaagtc tgtcaatcag ggtaattcgc tatcacacaa ggcgatcatt tatctgcatc
     1621 aacgccggac aaccatgcga tggtagccat acggccgcaa agcgggtcgc gccgatagga
     1681 ataaaaaaag tccggctggc cataggtgca ataaccgctt aattcaatat ccgcaacccc
     1741 caactgatat aagcgatgac gcgccaagcc gggcaaatcg gccagccatt tcctgttcgg
     1801 gattttttca gcgaagaaaa cccgcgatag cccatcccga tccacaaatg cggaaaagac
     1861 atcggcgcct acttcgaaat ggcgctggct gatggctggc ccaacccagg cgtgccactg
     1921 ggcgtgtgcc gggtggggat gcgcggcacg caatcgtgca agggtctgct ccagcacccc
     1981 gccggcgagt ccacgccagc ccgcgtgcgc cactccgagc accagcccgt ctgtggccgc
     2041 aataacgatc ggcaggcaat cagccgtcat aatcgccaaa acacgatcga cgcgggtcgt
     2101 aacagcagcg tcggcgacgg ggggaccccc gatggcgcca ttatcgacgt cagcatcgaa
     2161 tacgctggcg ccatgcacct gattcagcca aagcggctcg ccaggcaagc ggtcgcccaa
     2221 tcggctccgg ttttcgacaa cgcacgaagg ct
//
LOCUS       DATCXU010000135         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_779970,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000135 DATCXU010000000
VERSION     DATCXU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779970"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VIR04_02990"
     CDS             <1..677
                     /locus_tag="VIR04_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY9459748.1"
                     /translation="RLAALKRYYLWALRQGRVSKDPCLTLRAAKQAARFPQTLSEAQV
                     DALLRAPDVHTTLGLRDRAMLETLYATGLRVTELTTLNVLDASLNEGVVRVIMGKGGK
                     DRLVPLGAEALYWIDQYLKQARVELLKQRHCDALFVTARAAAMTRQSFWLIVKKYAFL
                     ADIHIPLSPHVLRHAFATHLLNHGADLRVVQLLLGHADISTTQIYTHVAHERLKALHA
                     KHHPRA"
     gene            688..1182
                     /locus_tag="VIR04_02995"
     CDS             688..1182
                     /locus_tag="VIR04_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEY9459749.1"
                     /translation="MNKSKHVSETPAVEFLKHHKVPFGEHTYNYIDHGGAPEAARQLG
                     VDPHAVAKTLIMEDEHAHPLIVLMHGDYDVSTKNLARQTGAKKISPCKPEVAQKHSGY
                     QVGGTSPFATRKKMPVWVEASLLEYPTVYLNGGRRGYLISVPSEILTTLLGATPVTVS
                     LPKN"
     gene            complement(1191..1955)
                     /locus_tag="VIR04_03000"
     CDS             complement(1191..1955)
                     /locus_tag="VIR04_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY9459750.1"
                     /translation="MTLQFFGGLFPSLTVFVMLCAATACAGAFRSFAGFGGGLMLAPL
                     YGLFMPPTDVLVVLLLLNLVTTVQMLPQVLKSVQWKLVISLAVPSLFGIPLGLLALHF
                     IDPMSMRKLIAAIVTVMSAILLTGWCYKGRRGHLQNGIAGLTSGSLTAIGAIGGPPVI
                     LYLLSSKDYGPTVLRSVFIAYFGIAIVATLTPLLFMGSITMAQAAHAASLLPVYLLAT
                     AVGTIAHQRMAGSDDGSIRRISLLVLLAIGVVTFFV"
     gene            2174..3340
                     /gene="bioF"
                     /locus_tag="VIR04_03005"
     CDS             2174..3340
                     /gene="bioF"
                     /locus_tag="VIR04_03005"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY9459751.1"
                     /translation="MLDLNHIRAQLTQLQEQQLMRTRRIVSGPQGPVLNIDDRQYLAF
                     CSNDYLGLANHPTLIAAAHAGLDAYGVGASASALISGHTRVFQELETTLADFVGMPRA
                     LHFSTGYMANLGIIPALVGAGDAVFSDQLNHACLIDGARLSRAEVKIYPHADTQRLEQ
                     LLKESTSPSKLVATDAVFSMDGDIAPLPKILALCEQYNAWLLVDDAHGFGVLGPQGQG
                     SLAHFGLASPRILYMGTLGKAAGVCGAFVAGDATLIEWLMQRAHTYVFTTASPPLLAS
                     ALLATLELVRTEQWRRQHLRQLVWQLRTGLAELRWSLLPSETAVQALVVHDDQLALDL
                     MEGLRERGIWVPAIRPPTVPKNTARLRISLSAAHTTQQVDFLIEGLHALAKHYD"
     gene            3333..>3666
                     /locus_tag="VIR04_03010"
     CDS             3333..>3666
                     /locus_tag="VIR04_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY9459752.1"
                     /translation="MIKDLETEDRSDMARRSLRSVWHPCTQMQHHESTPLVPVSSGRG
                     AWLYDANGKRYLDAISSWWVNVFGHANPRINAALKEQLDTLEHAMLAGLTHEPVIRLS
                     EKLAALTGN"
BASE COUNT          883 a         1065 c         1008 g          710 t
ORIGIN      
        1 gccggctggc ggcgttgaag cgctattacc tgtgggcctt gcgccaaggg cgggtcagca
       61 aggatccttg cctgacgctg cgtgccgcaa aacaggcggc gcgctttcca caaacgcttt
      121 cggaagccca ggtcgacgca ctgcttcgcg ctcccgacgt tcatacaaca ttgggcttgc
      181 gggatcgcgc gatgctcgaa acgctgtacg ccaccggctt gcgggtcacc gagttaacga
      241 ctcttaacgt actggatgca agtctgaacg agggcgtggt tcgcgtcatt atgggaaaag
      301 gaggcaaaga tcgcctggtg ccgcttggag ccgaagcctt gtactggatc gatcaatacc
      361 tgaagcaggc gcgcgtcgaa ttgttgaaac aacgccactg cgacgcgctg ttcgtcacag
      421 cacgggccgc cgccatgacg cggcagtcgt tctggctaat cgtcaaaaaa tatgcttttt
      481 tggctgacat acacataccg ttgtcgcctc atgtgctgcg ccatgccttt gccactcatt
      541 tgctcaacca tggcgccgac ttgcgcgtgg tgcagcttct gcttggacac gccgacattt
      601 ccaccaccca aatttatacc catgtggcgc acgaacgact gaaggcgctg cacgccaagc
      661 accacccacg tgcctgaagg aacacacatg aacaaaagca aacacgtttc cgaaaccccc
      721 gccgtcgagt ttctcaaaca ccacaaagtt cccttcggcg aacacaccta taactacata
      781 gaccacggcg gcgcgcccga ggcagcgcgg caactgggcg tcgatcccca tgccgtggca
      841 aaaaccctga ttatggaaga tgaacacgcg catccgctca tcgttctgat gcatggcgac
      901 tacgacgtat ccaccaaaaa cctggcgcgt cagaccggcg ccaaaaaaat cagtccatgc
      961 aagcccgaag tggcgcaaaa acattcgggc tatcaggttg gcggcacgtc gccgtttgca
     1021 acacgcaaga agatgcctgt ctgggtagag gcaagcctgc tggagtaccc caccgtctat
     1081 ttgaacggcg ggcggcgcgg ctatttgatc agcgtgccgt cagaaatatt aaccaccctg
     1141 cttggtgcga cgccggtcac tgtaagcctg cccaaaaatt gaaggccgcg ctacacaaaa
     1201 aatgtaacga ccccaatagc cagtaaaacc agtaaagaaa ttctgcgtat ggacccatcg
     1261 tcgctgcccg ccatccgctg atgcgcgatt gtcccgacag ccgtcgccag gagataaacc
     1321 ggaagtagcg acgcagcatg ggccgcctgt gccatagtga tcgaacccat gaacagcagc
     1381 ggcgtcaggg ttgccacaat ggcgatcccg aagtatgcaa tgaaaacaga gcgcaacacc
     1441 gtcgggccat agtctttgct ggaaagcaga taaagaatga caggagggcc accaatcgcg
     1501 ccaatggcgg taagagagcc gctggtcaat cccgcaatgc cattctgcag atgcccgcgg
     1561 cgccctttgt aacaccaccc tgtaagcaat attgccgaca tcacggtgac gatcgcagcg
     1621 atcagctttc gcattgacat gggatcaatg aagtgtaaag ccagcaggcc tagcgggatg
     1681 ccaaacaagc tgggaacagc cagggaaata accagcttcc attggaccga cttaagaacc
     1741 tgcggcaaca tttgcaccgt ggtgacaagg ttcagcagca gaaggacaac cagcacatct
     1801 gtgggaggca tgaacaagcc ataaagcggc gcgagcatca agccgccccc aaacccggca
     1861 aaactgcgga aggcccctgc gcatgcggta gcggcgcaca acatcacaaa caccgtcaat
     1921 gacggaaaaa ggccgccaaa aaattgcaat gtcatgtcag gcggcgcgcc tcaatttaaa
     1981 atcgtgggtt ccgcctgcgc gaggtatgtc ttccaacagc atcgaaggta aaattttcaa
     2041 cgaacaagtg atgcagttta cgccagtcac acgccagcca gttttcagag caacgggttt
     2101 aagcttgccc gatatactgg ccaccttgcc gattccgttt caggtatatc ggcgccgctc
     2161 caagagactt taaatgttgg atttgaatca cattcgcgcc cagttgacgc aattgcaaga
     2221 gcaacaacta atgcgcacac ggcgtattgt cagcggcccg caagggcccg tattaaatat
     2281 agacgatagg caatacctgg cattttgcag caacgattat ctggggctgg ccaaccatcc
     2341 cacgctgatt gccgccgccc atgcgggcct ggacgcttat ggcgtaggcg ccagcgcatc
     2401 agcattgatc agcggtcaca cgcgcgtatt ccaggaactg gaaacgacgc tggccgattt
     2461 cgtcggcatg ccgcgcgcct tgcatttttc gacgggttac atggccaacc tgggaatcat
     2521 ccccgccctg gtcggagccg gtgatgcggt gttttcagac caactcaacc acgcttgcct
     2581 gattgacggc gcaaggctat cgcgcgcaga agtcaaaatc tatccccacg ccgacacaca
     2641 acgtctggaa cagttgctga aagaaagcac cagcccgagc aagctggtag cgaccgatgc
     2701 cgtgttcagt atggatggcg acattgcacc cttgccaaaa atactcgcat tgtgcgaaca
     2761 atataatgcg tggctgctgg tggatgatgc ccacggtttc ggggtccttg ggccgcaagg
     2821 acaaggcagt ctggcccatt tcgggctggc ctcgccccgt atcctgtata tgggaacatt
     2881 gggcaaggcc gccggcgtat gcggcgcgtt cgtggccggc gatgcgaccc tgatcgagtg
     2941 gctgatgcag cgggcgcata cttacgtatt caccactgcc agcccgccat tgctggccag
     3001 cgccttgttg gcaacgcttg aactagtgcg aaccgaacag tggcgacgcc agcatttacg
     3061 gcaattggtc tggcaactgc gcacgggcct ggcagaattg cgctggtcgt tattgccctc
     3121 ggaaacggcc gttcaagcct tggtggtgca cgacgaccaa ttggctctgg acttgatgga
     3181 aggcctgcgc gagcgcggta tatgggttcc ggcgatacgg ccgccaacgg tgccgaagaa
     3241 tacggcgcga ttgcgtattt cattatcggc cgcgcataca acccaacagg tcgatttttt
     3301 aattgaagga ttgcatgccc tggccaaaca ttatgattaa agatttagag accgaagacc
     3361 gttcggacat ggcgcgccgc agcctacgca gcgtgtggca tccctgcacg caaatgcagc
     3421 atcacgagtc gacgcctttg gttcctgtta gcagcggtcg cggtgcctgg ctgtacgacg
     3481 ccaatggcaa acgctatctg gacgccatca gctcatggtg ggtcaatgtg tttggtcacg
     3541 ccaacccgcg tattaatgct gcattaaaag agcaactcga cacgctcgaa cacgcgatgc
     3601 tggccggttt gacccacgag cctgttatcc gtctgtccga aaaactggcg gcattgacgg
     3661 gcaacg
//
LOCUS       DATCXU010000136         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_366803,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000136 DATCXU010000000
VERSION     DATCXU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366803"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VIR04_03015"
     CDS             <1..851
                     /locus_tag="VIR04_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY9459753.1"
                     /translation="AAQAATAPAASSVHAMLASPDNIAPPKPVAVPAPANSADQDTRK
                     ADTDNELTETRRTGHWTETSPTLKAGNVASNQASATMVPNALALPVHRADGTNDAPTQ
                     IVAPAISSIGSLVDSSMLTATTAASQGNMQMTSLGIGTPLQNAQWSNDFARQLTTLAG
                     NGATHTAELSLNPPDLGPLRISISVSDNVAQAVFMSPHAAVRQAVENALPQLQQTLAN
                     AGISLGQASVSDQSNRQDAFDQVQPVKRKLSASAIAGIGSAGNGPPVHSTQRSKALEG
                     LVDTFA"
     gene            887..1384
                     /locus_tag="VIR04_03020"
     CDS             887..1384
                     /locus_tag="VIR04_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY9459754.1"
                     /translation="MPPIAHSPFRKAITNILTVLIVMMGSVGATVFYMSHSDQPADAA
                     KKNPTQKNAAKSPSSVPAPIFVELEPFTVTLDGESGSRILYAAITLRVEDDASRKLLA
                     SYMPEVRDRVLLLLSEQDAAVVQKPKIRQQLAQELIKALEAVYKPQPKQPKITSVLFT
                     AFVVQ"
     gene            1385..2395
                     /gene="fliM"
                     /locus_tag="VIR04_03025"
     CDS             1385..2395
                     /gene="fliM"
                     /locus_tag="VIR04_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY9459755.1"
                     /translation="MPYQNFLSQDEVDALLAGVTGESTESKPAASDQTGVRAYDLSSP
                     DRVVRHRMQTLELINERFARKLRSLLLNFMRRNADITVGAIRILKYSDFERNLPVPSN
                     LNMVAIKPLRGATLFTYDPNLVFLVIDSLFGGHGRYNTRIEGRDFTTTEQRIIRRLLN
                     LTLDSYGAAWQTIYPVELEYIRSEMHTKFASITSGNEIVVVSTFNIELGASGGKLNIC
                     IPYSTIEPLRDLLTQPLQDANANSIDQRWLHQLSRQVRSADVELTVDFTHISTSIAAL
                     LKMRVNDVLPIEVPAAVTGVVGGVPIMECTYGTFNGRYALRVQKLLTHSESELTQATQ
                     HD"
     gene            2388..>2651
                     /locus_tag="VIR04_03030"
     CDS             2388..>2651
                     /locus_tag="VIR04_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459756.1"
                     /translation="MTEPNLQPDKEIPATADSADAEWAAALSEQAAQNKQPTQADGLA
                     DAASVQADDWANALAEQTAAATMPAETSSASATPIPSTAAMAAQ"
BASE COUNT          663 a          847 c          682 g          459 t
ORIGIN      
        1 ccgccgcaca ggccgcaacc gcgccagcgg catcaagcgt acacgcgatg ctggcatcac
       61 ccgataatat cgcacccccg aagccggtcg ccgtaccggc gccagccaat tccgcggatc
      121 aggacacccg aaaagcggat accgacaacg aactaaccga gaccaggcgc actggccact
      181 ggacagaaac ctcgccaacg ttaaaagccg gcaatgttgc cagcaaccag gcgtcggcta
      241 ccatggttcc aaacgctctg gcacttcccg tccaccgtgc agatggcacg aacgacgccc
      301 caacccaaat agttgctccg gcaatctcca gtatcggctc gctcgtggac agctcaatgc
      361 tgacagcaac cacagccgcg tcgcagggaa atatgcaaat gacaagcctg ggtatcggaa
      421 cgccgctgca aaacgcacaa tggagtaacg acttcgcgcg ccaactcacc acactggcgg
      481 ggaatggcgc aacgcacacg gccgaattaa gcctgaaccc tcccgacctt ggccctttgc
      541 ggatttcaat cagcgtaagc gacaacgtcg cgcaggcagt cttcatgtcg ccccacgcgg
      601 cggtacggca agccgtcgaa aacgcgctgc cgcaattaca acaaaccttg gcgaacgccg
      661 gcatttcgct gggacaggcc agcgtcagcg accagtcaaa tcggcaagac gcatttgatc
      721 aagtgcaacc ggtaaaacgc aaattgtccg cgagcgccat agcagggatc ggatcagccg
      781 gcaacgggcc gccagtgcac tcaacacaac gcagcaaggc tcttgaagga ctggtcgaca
      841 ccttcgccta acgacccaca ccaagctatc aaccacccaa accatcatgc cacccatcgc
      901 acatagtccg ttccgcaaag ccatcacgaa tatccttact gtactgattg tcatgatggg
      961 aagcgttgga gcgaccgtat tctatatgtc gcactcggat cagccggcgg atgccgcaaa
     1021 aaaaaacccc actcagaaaa acgcggccaa atctccatcc agcgttcccg cacccatatt
     1081 tgtcgaactg gagccgttta ccgttacgct ggacggcgaa agcggaagcc gcattttgta
     1141 cgccgccatt accttgcggg tggaagacga cgcgagccgg aaactcctag cgagttatat
     1201 gccggaagta cgcgatcgcg tgttgctgtt gctatccgag caggacgcgg cagtcgtcca
     1261 aaagccgaaa atccggcaac aactggcgca agaattgatc aaagcgctcg aggccgtgta
     1321 caagccgcaa ccgaaacagc ccaaaatcac gagtgttttg tttaccgcct ttgtggtcca
     1381 gtaaatgccc tatcaaaact ttctctcgca agatgaagtc gacgcgctgc tggcgggcgt
     1441 aacgggggaa agcacagaat caaaacctgc cgccagcgat cagacggggg tacgcgccta
     1501 tgatttgagc tcgcccgacc gggtcgtgcg gcacaggatg cagactctgg aactaatcaa
     1561 cgagcgcttc gcccgcaagc tgcgctcgct gctgcttaac ttcatgcggc gcaacgccga
     1621 catcaccgtc ggcgccattc gcattctgaa gtactcggac ttcgaacgta atttgccggt
     1681 gccaagcaac cttaatatgg tcgcgataaa gccgttgcgc ggcgcgacat tattcaccta
     1741 cgaccccaac ctggtgtttc tggtcatcga cagtctgttc ggaggccacg gccgctacaa
     1801 tacccgaata gaggggcgcg atttcaccac gaccgaacag cgcatcatca ggcggcttct
     1861 taacctgact ctggacagct atggggccgc atggcaaacc atctaccccg ttgagctgga
     1921 atatatccgc tccgaaatgc ataccaagtt tgccagcatt accagcggca acgaaatcgt
     1981 cgtggtatcc actttcaaca ttgaactggg cgcctcgggt ggcaaactca acatctgtat
     2041 tccctactcc acgatcgaac ccctgcggga tttactgacc cagcctttac aagacgccaa
     2101 cgcaaattcc attgaccagc gctggttgca tcagctttcg cgacaagttc gaagcgcgga
     2161 tgtcgaactt accgtcgact tcacacacat ctcaaccagc attgccgccc ttttaaaaat
     2221 gcgcgtgaac gacgttttgc cgattgaagt ccccgccgct gtaacgggcg tggtcggcgg
     2281 cgttccgatt atggaatgta cttacggaac ctttaacggc cgatacgcgc ttcgtgtgca
     2341 aaaactgctg acgcactcgg aatccgaact aacccaagcg acccaacatg actgaaccca
     2401 atctacagcc cgacaaagag attccggcga cggccgacag cgccgacgcc gagtgggccg
     2461 cggccttatc cgaacaggcc gcgcaaaaca agcagccgac tcaggccgat gggctggcgg
     2521 atgcggcctc ggtccaggct gacgactggg ccaacgctct ggccgaacaa acagccgccg
     2581 ccaccatgcc cgccgagaca agctccgcgt cggcgacacc aattccgtca acggcggcga
     2641 tggcggcgca a
//
LOCUS       DATCXU010000137         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1126622,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000137 DATCXU010000000
VERSION     DATCXU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126622"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="pstS"
                     /locus_tag="VIR04_03035"
     CDS             complement(<1..668)
                     /gene="pstS"
                     /locus_tag="VIR04_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY9459757.1"
                     /translation="MYKRALLNLSAGITLGAFTFMAQAANITGAGASFPYPVYAKWAA
                     KYHETSGNQVNYQSIGSGGGQQQIIAKTVDFGASDDPMPGDALEKNGLFQFPAIIGGT
                     VPVINIRGLKPGELKLSGSVLADIFLAKITKWDDAAIKALNPGLALPSASIIVVHRAD
                     GSGTTFGWTNYLSKVSTAWKEQVGEGKAVKWPTGQGGKGNEGVAAYVRQLKNSIGYVE
                     YAYA"
     gene            complement(771..2873)
                     /gene="ppk1"
                     /locus_tag="VIR04_03040"
     CDS             complement(771..2873)
                     /gene="ppk1"
                     /locus_tag="VIR04_03040"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY9459758.1"
                     /translation="MDSPMHAVLPETVSPNRSVLLNRELSLIKFNERVLALADNPRVP
                     LLERLRYLCIVGSNLDEFFEIRISSLKEQQRQNATLAGVDGLTPGEAFLQVQAAAHRL
                     VRHQNSLLMDQVLPDLMAEGIGLFDADAWTDEIREWAYGVFNRDVMPMLTPIGLDPAR
                     PFPRVYNKSLNFIVPLSGRDAFGRQAAIAIVQAPRALPRVIKMPSDICGIPDGYVLLT
                     AVIGAFVGDLFPGLDVKGCYQWRVTRNSDLFVDEEEITNLRQALQGELSQRNFGAAVR
                     LEIAESMPANLVAFLQHEFALDEHDTYWVSGPVNITRLNQLCNRVDRPDLSFPVYRPA
                     IPAPFDVVSDKPDELFAAIAQQDRLLHHPYQSFQPVINFLTAAAFDPAVVAIKQTIYR
                     TGEDSELMRLLLVAAKAGKEVTVVVELMARFDEQTNINWAAKLEAVGAHVSYGVVAHK
                     THAKMALVLRRERGRIRRYGHLGTGNYHPRTAKLYTDFGLLTANQRMCEDMDKVFSLL
                     TGLGARRPLKLLLQSPFTLHEAIIAMIRAEAINAKAGKKARIMAKMNSLLEPIVIEEL
                     YKASQAGVKIDLVVRGACALRAGVQGLSETIRVRSIIGRFLEHARVFYFYNDGAENVY
                     LSSADWMDRNFFRRVEICFPVLDKALKKRVISEALTYAWRDNQLAWRAQPDGDYSKVN
                     GRGAAFNLHTYLMQRLGA"
BASE COUNT          700 a          877 c          776 g          636 t
ORIGIN      
        1 ttggcgtagg catactcgac gtagccgatg gaattcttca gctggcgaac atacgcggcc
       61 acaccttcgt tgcccttgcc gccctgcccc gtcggccact tcacggcctt gccttcaccg
      121 acttgctctt tccaggcagt cgacactttc gataggtagt tggtccagcc gaaagtcgtg
      181 cccgagccgt cggcgcgatg caccacaata atcgacgcgg aaggcagagc cagacccggg
      241 ttaagcgctt taatcgcggc gtcgtcccat ttggtgatct tggccaggaa tatatcggcg
      301 agcacagaac ctgacaattt gagctcgccg ggcttgagac ccctgatgtt aatcaccgga
      361 acggtgccgc cgatgattgc gggaaactga aataaaccgt tcttttccag cgcatcgccg
      421 ggcatgggat cgtcagacgc gccaaaatcc acggttttgg caatgatttg ctgttggcct
      481 ccaccggaac cgatggattg gtaattaacc tgattaccgc tcgtttcgtg atatttggcc
      541 gcccatttcg catacacagg atagggaaac gaggcgccgg cgccggtgat attggccgct
      601 tgggccatga acgtgaacgc cccaagggta atgcctgccg aaaggttaag taaagcgcgt
      661 ttatacatca gggatcctct tgaatgtgtc aacggttggc ggcgtgtatg accgcacaac
      721 cgcatactag acgcccaaca tgacaaacgt gtgacagcct tgaacgaaaa tcaggccccg
      781 agccgttgca tcagatacgt atgcagatta aacgccgcgc cgcgaccatt cactttgctg
      841 tagtctccgt cgggttgagc ccgccatgcc aactgattat cgcgccaggc atacgtaagc
      901 gcctcgctaa taacgcgctt tttcaatgcc ttgtccagca ccgggaagca aatctcgaca
      961 cgccggaaaa aatttctgtc catccagtcg gccgaagaaa gatagacatt ttcggcgccg
     1021 tcgttgtaga aataaaacac ccgggcgtgt tccaaaaagc gcccaatgat cgaacgcacg
     1081 cgtatcgtct cagacaaacc ctgaactccg gcgcgcaaag cgcaagcgcc gcgcacgacc
     1141 agatcgattt ttacgccagc ctgacttgcc ttgtaaagtt cttcgatgac aatcggttcc
     1201 agaagcgaat tcatttttgc cataattcgg gctttcttcc ctgccttggc attgatcgcc
     1261 tcggcgcgga tcatggcgat tatggcttca tgcaacgtaa agggtgactg caacagcagc
     1321 ttgagcggtc ggcgcgcccc caatccggtg agcaaagaga acaccttatc catgtcttcg
     1381 cacattctct ggttcgcggt cagcaggccg aaatcggtat acagcttggc cgtacgtgga
     1441 tggtagttgc ccgtgcccaa atgcccatac cgccttatcc gtcctctttc acgccgcaaa
     1501 accaacgcca tttttgcatg tgtcttgtgc gccaccacgc cgtaactgac gtgagcgccc
     1561 accgcttcaa gcttggcggc ccaattgata ttggtctgtt catcgaaacg cgccatcagt
     1621 tcaaccacca ccgtgacttc ttttccggcc ttggcggcca ccaatagcaa acgcatcagt
     1681 tccgagtctt cgcctgtgcg ataaatcgtt tgctttatag ccactaccgc agggtcgaat
     1741 gccgccgccg tcaagaaatt aatgaccggc tggaaggact ggtaggggtg atgaagcagg
     1801 cggtcttgct gtgcgatggc ggcaaacaat tcgtcaggct tgtcggacac cacatcgaaa
     1861 ggcgccggga tagccggccg gtagacagga aaggacaagt cggggcggtc aaccctgtta
     1921 cagagctgat tcaaccgcgt tatgtttacc ggaccgctaa cccaataagt atcgtgttcg
     1981 tcgagggcga attcatgctg aaggaaagct accagattcg ccggcatgga ctcggcgatt
     2041 tcaaggcgga cagcggcgcc gaagttccgc tgcgacaact caccctgaag cgcctggcgc
     2101 aaattggtga tttcctcctc gtcgacgaac aaatcgctat ttctggtaac acgccactga
     2161 taacagcctt tgacatccag ccccggaaac aggtcgccga caaacgcgcc gatcaccgcc
     2221 gtgagcaaaa cgtatccatc aggtatgccg caaatatcgg aaggcatctt gatgacacgc
     2281 ggcaatgccc ggggagcctg gacaatcgca atggcggcct gccggccaaa cgcatcccgg
     2341 ccggacaggg gcacaataaa gttcaggctc ttgttgtata cgcgcggaaa aggccgggcg
     2401 ggatcaagcc cgatcggcgt gagcatgggc atgacgtcgc gattaaaaac gccgtaagcc
     2461 cactcgcgta tttcgtccgt ccatgcgtcc gcgtcgaaca agccaatgcc ctcggccatc
     2521 aggtcgggaa gaacctgatc catcagcaga ctgttctggt gcctgaccag acgatgcgcc
     2581 gccgcttgta cctgcagaaa cgcttcgccc ggcgtaaggc cgtccacccc cgccaaggtc
     2641 gcattctggc gctgctgttc tttcaggctg gaaattcgaa tttcgaaaaa ctcatccaga
     2701 ttcgatccga caatgcacag ataacgcagg cgttcaagca aaggcacgcg cggattgtcg
     2761 gccaatgcca ggacgcgctc gttgaacttg atcagagaca attcacgatt gagcaatact
     2821 gaccggttcg gcgatacagt ttcaggcaac acggcatgca taggactatc catcacacta
     2881 tattacgcaa tctcgcgcct cattttcacg acatgccctg aactctactc aaaatcgccg
     2941 catttcgcac acattttgcc attttcccta acggaacgaa ttcggtcct
//
LOCUS       DATCXU010000138         5206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_360974,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000138 DATCXU010000000
VERSION     DATCXU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360974"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VIR04_03045"
     CDS             complement(<1..129)
                     /locus_tag="VIR04_03045"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY9459759.1"
                     /translation="MSLEFFPPRDIAAQEKLVRSAKQLLALHPSYVSVTFGAGGSTR"
     gene            complement(159..497)
                     /locus_tag="VIR04_03050"
     CDS             complement(159..497)
                     /locus_tag="VIR04_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY9459760.1"
                     /translation="MEIILVWILNAVALLVVAYILPGITVASFGSALVAALVLGLLNT
                     LVKPLLILLTLPITVVTLGLFLLVLNALVFWFAGSILKGFHVNGFWWAMLGAVVYSLV
                     SGLLSRLIYQ"
     gene            complement(539..1957)
                     /gene="ahcY"
                     /locus_tag="VIR04_03055"
     CDS             complement(539..1957)
                     /gene="ahcY"
                     /locus_tag="VIR04_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY9459761.1"
                     /translation="MNTVAKAAFTDYKVADIGLAAFGRREIAIAETEMPGLMATREEF
                     VATQPLKGARIAGSLHMTIQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAADGT
                     AVFAVKGESLEDYWQYTHQIFTWPDGKNANMILDDGGDATALLHLGAKAEANRAVLDK
                     PSSEEERVLFAAIKAQLARDSKWYSTRLAQIKGVTEETTTGVHRLYQMSKKGELKIPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVCGFGDVGKGCAQALSAL
                     RAQVWVTEVDPICALQASMEGYKVVTLDEAADKADIFVTATGNYHVIKREHMDRMKDQ
                     AIVCNIGHFDNEIDVASIEELQWEEVKPQVDHIIFPDGKRIILLAKGRLVNLGCATGH
                     PSFVMSASFTNQTIGQIELFTRTDQYKTGQVYVLPKRLDEKVARLHLKKLGVHLTTLR
                     PDQAEYINVPVEGPYKADYYRY"
     regulatory      complement(2030..2133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(2188..2400)
                     /locus_tag="VIR04_03060"
     CDS             complement(2188..2400)
                     /locus_tag="VIR04_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY9459762.1"
                     /translation="MDAVSVRDESRSLKPSDSPCVAKCSTLYDDVCRGCGRTTMEVAN
                     WVFLTEEEKQAVWTRIKAQGYPQSKC"
     gene            complement(2464..3627)
                     /gene="metK"
                     /locus_tag="VIR04_03065"
     CDS             complement(2464..3627)
                     /gene="metK"
                     /locus_tag="VIR04_03065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY9459763.1"
                     /translation="MVNNDFLFTSESVSEGHPDKVADQISDSILDAILTQDPHARVAA
                     ETLCNTGLVVLAGEITTTANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQS
                     PNIAQGVDRSPEEILNQGAGDQGLMFGYACDETPDLMPAPIWYAHRLVQRQSELRKDG
                     RLPWLRPDAKSQVTFRYAKGKPVEVDTVVLSTQHSPDISQAEIKEAVIENIIKPSFPA
                     GLLTAKTKYLVNPTGLFVIGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVD
                     RSAAYAARYVAKNIVAAGLARQCQVQVSYAIGVAEPINITVYTEGTGVIPDDQIAKLV
                     LEHFDLRPKGIINMLDLLRPIYAKTAAYGHFGRSEPEFSWEAADKVQVLKKAA"
     gene            3871..4743
                     /locus_tag="VIR04_03070"
     CDS             3871..4743
                     /locus_tag="VIR04_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY9459764.1"
                     /translation="MLLVLFRLLAYLPLPVLHGIGRIAGRMVYAMPGKYRQRLQSNAR
                     QAGYPQPAFARRAAAETGAMIMELPKVWLRNEQGLRKATTSDGAVVQAALDEHRGILY
                     LTPHLGCFEITARYLTRHGPLTVMFRPARKAFLGSIIEKGRATSNLNAVPANLHGVRE
                     FLRTLKRGGAVGMLPDQAPSNGDGVWAPFFGRPAYTMTLAGKLAVQTGVAVILTAGER
                     LPAGRGWRIHYVRLPEPFPDTPEAQATLINAAMEALIRRFPEQYLWSYNRYKTPKGAP
                     KPSGQPGVKPDVTP"
     gene            4740..>5206
                     /locus_tag="VIR04_03075"
     CDS             4740..>5206
                     /locus_tag="VIR04_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY9459765.1"
                     /translation="MKGGKLRLLRKVFTYFGNCSDATRLRWGRALGWLTRKLLRSRVH
                     IVRTNLALCFPERPAKEREALLRRHFHLLAQSYIDRGLCWFGEASHILDTVQITGLEH
                     LDKLLNEQRRVIMLAPHFIGIDAAGTRITLHLKRVASMYTPQSDPDVDTLVREG"
BASE COUNT         1157 a         1531 c         1409 g         1109 t
ORIGIN      
        1 ccgcgtcgac ccgcctgccc cgaacgtcac gctcacgtag ctggggtgca aggccagcaa
       61 ctgcttggcc gaccgaacca gtttttcttg cgccgcaatg tcgcgcggcg gaaaaaactc
      121 caggctcatg acccgcggag gattcgtttg cgtgggattc attgataaat caacctggaa
      181 agaagcccgg acaccaggct atatacaacg gcgcccaaca tggcccacca gaagccattc
      241 acgtgaaagc ccttcagaat cgagccggcg aaccagaaca ccaacgcatt aagcaccagt
      301 aaaaacagcc ctaaagtcac caccgtaatg ggcagcgtca gcaaaatcag caggggcttg
      361 accaaggtat tgagcaagcc caataccaat gccgcaacaa gcgccgagcc aaacgatgca
      421 acggtaatcc ccggcaagat ataggccaca accagcaagg ccaccgcatt cagaatccag
      481 acaagaatga tttccatcat gtgactccca ttaatgcatc aatgcaccgc ggtatcgcct
      541 aatagcgata gtagtccgcc ttgtagggcc cttccaccgg cacgttaatg tactcggcct
      601 ggtccgggcg caacgtcgtc aaatgcacac ccagcttctt gagatgcagg cgagccactt
      661 tttcgtcaag ccgtttgggc aatacgtaaa cctgccctgt cttgtattga tcggtgcgcg
      721 tgaacaattc gatctggcct atcgtttgat tcgtgaacga cgccgacatc acaaacgaag
      781 gatggcctgt tgcgcaaccc aggttaacca aacgaccttt cgccaacaaa atgatgcgtt
      841 tgccatccgg gaagataatg tggtcgacct gaggcttgac ctcttcccac tgcaattctt
      901 caatggacgc cacgtctatt tcattatcga agtggccaat attgcacacg atggcctggt
      961 ctttcatgcg atccatgtgt tcgcgtttaa tgacgtggta atttccggtg gcagtgacaa
     1021 agatgtcggc tttatcggcc gcttcatcca gcgtgaccac tttgtagcct tccatggacg
     1081 cctgcaaggc gcaaatagga tcaacctcgg tcacccaaac ctgggcccgc aacgccgaca
     1141 gcgcctgggc gcagcctttg cccacgtcgc caaaaccgca caccaccgca accttgcccg
     1201 ccaccatgac gtcagtggcg cgtttgatgc cgtcaaccag cgactcgcgg cagccgtaca
     1261 gattgtcgaa cttggacttg gtgaccgaat cgttaacgtt gatggccgga atcttcagct
     1321 cgcccttttt agacatctga tacaggcgat gcacgcccgt tgttgtttct tcggtcaccc
     1381 ctttaatctg ggccagacgt gtcgaatacc atttcgaatc gcgtgccagt tgcgccttga
     1441 tcgcagcaaa cagcacacgc tcttcttcgc tggacggttt gtccagcacc gcgcgattgg
     1501 cttcggcctt ggcgccaaga tgcagcaacg ctgtggcatc gccgccatca tccagaatca
     1561 tgttggcgtt cttgccatcg ggccaggtga aaatctggtg ggtgtattgc cagtagtctt
     1621 ccagcgattc gcccttgacg gcaaacacgg ccgtgccgtc ggccgcaatg gccgccgcgg
     1681 cgtgatcctg ggttgaaaaa atattgcagg acgcccagcg cacttcggct ccaagcgcca
     1741 ccagcgtctc gatcagcaca gccgtttgaa tcgtcatgtg caggctgccc gcgatacgcg
     1801 cgccctttaa tggctgagtg gcgacaaatt cttcgcgcgt ggccatcagg cccggcattt
     1861 cagtttcggc gatcgcaatt tcgcgacggc cgaaggcggc cagcccaata tcggcaactt
     1921 tataatcggt gaatgcggct ttggcaacag tgttcatggt ggaactccaa taaacctgtt
     1981 cgagcgaaag cgccgggatg gtttggctca ccttcgcctg cgaaacagga gatgatgagc
     2041 gccgttaatg caaaaaacga atcctgagcc tagcgagact gctgacgtct gaacgacttc
     2101 ggtgcaatgc tcgtcgcaac gctcctcagg aacgtcgaat tgtagcgtaa attaaaaagc
     2161 gccaacactt gccgtcgacg cctgcggtca acatttggat tgcgggtaac cttgcgcctt
     2221 gatgcgggtc cagacagcct gcttttcttc ttcggtaaga aagacccaat tggccacttc
     2281 catcgtggtt cggccacaac cgcggcagac atcgtcataa agcgtcgaac acttcgcaac
     2341 acaaggcgaa tcactgggtt tcaaactgcg gctttcgtcg cgaacagata ccgcatccat
     2401 ttgctgcccc ctgtctgatg cgcaaacagg gcgcggttcg accgaacggc gccctgatcc
     2461 aacctatgcg gctttcttta aaacctgcac tttgtctgcg gcttcccaac tgaattcggg
     2521 ttcgctacgg ccgaagtgac cataggccgc ggtcttggcg taaatcgggc gcaacaaatc
     2581 cagcatattg atgatgccct tgggacgcag atcaaagtgc tcaagcacca gtttcgcgat
     2641 ctggtcgtcg ggaataacgc cagtgccctc ggtgtaaacc gtaatgttga tcggttcagc
     2701 caccccgatg gcgtagctga cctgaacctg gcactgtcgt gcaagccccg cggccacaat
     2761 atttttggcc acataacgcg ccgcgtaggc ggccgagcga tccaccttgg atggatcttt
     2821 acccgagaac gcgccgccgc catgggggca ggcgccgccg taagtgtcga caataatttt
     2881 gcggccggtc aggccgcaat cgccttgcgg gccgccaatc acaaatagtc ccgtaggatt
     2941 gaccagatac ttggtcttgg ctgttagcag gccagccgga aaacttggct tgatgatatt
     3001 ttcgataacg gcttctttaa tttccgcttg cgaaatatcg gggctgtgct gggtcgacaa
     3061 caccacggta tcgacttcga ccggtttgcc tttggcatag cggaacgtga cttgcgattt
     3121 ggcatccggg cgcagccagg gcaggcgtcc gtctttgcgc aattcgcttt gacgctgcac
     3181 cagacggtgg gcataccaga tgggcgccgg cataagatcg ggggtttcgt cgcaggcata
     3241 accgaacatc agaccctgat cgccagcgcc ctgattcagt atttcttcgg gactgcgatc
     3301 aacgccctga gcaatgttgg gcgactgctt gtcgtacgcg accagcacgg cgcacccttt
     3361 gtagtcgatg ccgtattccg tgttgtcgta gccaatgcgc ttgatggtgt cgcgcgcgac
     3421 ctgaatgtaa tccacattgg cggttgtcgt gatttcaccg gccaatacaa ccagaccggt
     3481 gttgcacagc gtttcggccg caacccgcgc atgggggtct tgagtgagga tggcgtctag
     3541 aatggaatct gaaatctggt cggccacctt gtccggatga ccttcggaaa ctgattcgga
     3601 agtaaaaaga aaatcgttat ttaccacagc gatacgtcct taatagtggc cggaaatcca
     3661 gcctaaaggt tgaacggaac gcgttgtacc cagagaatgt gttgcagatt gacgaggcac
     3721 gacgctttag cggttttgga ctgcgccatt actaccaggg cgcagacatc gctccgcaag
     3781 ttgtcggtta actcagcgat aagtcgcatt ttaagcgaaa atacgaaaac gcactttttc
     3841 gcatcttcca catcaaggtt attgccccgc atgctattgg tcctgttccg gctgcttgcc
     3901 tatttgccgc tgcccgtttt gcacggcata ggccgcatcg cgggccgcat ggtgtacgca
     3961 atgcccggca agtatcgtca acgtctgcaa agcaatgcca gacaagcggg ttacccccaa
     4021 cccgcttttg cgcgccgggc ggcggccgaa accggcgcaa tgatcatgga actgcccaaa
     4081 gtctggctac gcaacgaaca aggtctgcgc aaagcgacaa ccagcgacgg cgccgtcgtt
     4141 caagccgcgc tggacgaaca ccggggcatt ctttacctta cgccgcacct gggctgcttc
     4201 gagatcaccg cgcgctatct gacacggcac ggcccgttga cggttatgtt tcggcccgcg
     4261 cgcaaagcct ttttgggttc aataatagaa aaagggcgcg ccacatcaaa ccttaatgcg
     4321 gttcccgcca atttgcatgg cgtgcgtgaa tttttgcgca ccctgaaacg tggaggcgcg
     4381 gtgggcatgc tgcccgacca ggctcccagc aatggcgacg gcgtatgggc gccgtttttt
     4441 ggcaggcccg cctatactat gaccctcgcg ggcaagctgg cggtccagac gggggtggcg
     4501 gtcatactca cagccggcga acgcttgccc gcagggcgcg gatggcgcat tcattatgtg
     4561 cggctgcccg aacccttccc cgataccccc gaagcacaag ctactcttat aaatgcggcc
     4621 atggaggccc tgatccggcg ctttcccgaa caatacctat ggagctacaa ccgatacaaa
     4681 acgcccaaag gcgcgcccaa gcccagtggc caacctgggg tcaagcctga cgtcacacca
     4741 tgaaaggcgg caaactacgg ctattacgca aagtattcac gtatttcggc aattgctccg
     4801 atgcaacccg gctgcgatgg ggccgggcgc taggctggct gacgcggaaa ctgttgcgtt
     4861 cgcgcgtgca catcgtcaga acgaacctgg cgctttgttt tccggaacgc ccggcaaagg
     4921 aacgggaagc gttgctgcgc cggcatttcc atttgcttgc gcaatcctat attgatcgcg
     4981 gtctgtgctg gttcggcgag gcctcgcaca tactggacac tgtccaaatc acagggctgg
     5041 aacatctgga caagttgctg aatgaacaac gccgcgtcat tatgctggcg ccgcatttta
     5101 tcggaataga cgcggcgggc acccgcatta ctcttcactt gaagcgcgtg gccagcatgt
     5161 atacgccgca aagcgacccc gacgtcgaca cgctggtgcg cgaggg
//
LOCUS       DATCXU010000139         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_379914,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000139 DATCXU010000000
VERSION     DATCXU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379914"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="ugpB"
                     /locus_tag="VIR04_03080"
     CDS             <1..948
                     /gene="ugpB"
                     /locus_tag="VIR04_03080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEY9459766.1"
                     /translation="LGAVASYYSSPDGKLVSMPFNSSTPVFYYNMDAFKKAGLDPQKP
                     PKTWDDVAQAATKLRAAGLECGYSTGWPSWVQLETFSAWHNAPYASKNNGFGGLDARL
                     EVNKPLFVRHMTFLAKMAKEGTFTYGGRGDAANALFISGKCGMFTGSSGMRASVKKNA
                     NFEFGTSPLPYYADVKGAPQNTIIGGASLWVFAKKSPEVYKGITKFFKFLASPEQAAA
                     WHQGTGYVPVTKAGYELTKKSGFYDKNPGTDVAVKELDATTTKNSRGVRLGYLPQIRD
                     IEDADMEQIFAGKVTPKQGLDNMVKRGDELLARFQKSVK"
     gene            1035..1916
                     /gene="ugpA"
                     /locus_tag="VIR04_03085"
     CDS             1035..1916
                     /gene="ugpA"
                     /locus_tag="VIR04_03085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEY9459767.1"
                     /translation="MEKRVVFNHKTLPYLLLAPQLAITVIFFFWPAGQAMWQSFRIED
                     PFGLSSQFAGLSNFIELFSNSDYINSFKVTAVFSVLVAVLGLSISLVLALMADRVVKG
                     AKLYKTLLVWPYAVATAVAGVLWLFMFSPSVGVIAVYLANLGFSWNPRLNSTDAMTLV
                     VLASVWKQISYNFLFFLAGLQSIPRSLIEAAAIDGASPMRRTMTIILPLLTPTTFFLL
                     VVNIIYAFFDTFAVIDIMTQGGPGNTTAILVYRVYNTGFLGLDIGSSAAQSVILMAIV
                     VALTIVQFRYVERKVNY"
     gene            1944..2786
                     /gene="ugpE"
                     /locus_tag="VIR04_03090"
     CDS             1944..2786
                     /gene="ugpE"
                     /locus_tag="VIR04_03090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEY9459768.1"
                     /translation="MIERRPYLDFFSHLVLLIGVAVIAFPLYLTFIASTQTAQEVAQA
                     PMSLLPGGHFLENYQTALFGGVGNTAPVARMMWVSTVMALVIAIGKISISMLSAFAVV
                     YFHFRFRMFFFWMIFITLMLPVEVRITPTYKVVADLGMLNTYAGLTLPLIASATATFL
                     FRQFFLTVPDELIEAARIDGAGPMRFFKDILLPLSSTTIAALFVIQFIYGWNQYLWPL
                     IITTKENMYPVVIGIKRMIAGGDSVTEWNLVMATALLAMIPPAAVVIFMQKWFVKGLV
                     DAEK"
     gene            2811..3899
                     /locus_tag="VIR04_03095"
     CDS             2811..3899
                     /locus_tag="VIR04_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEY9459769.1"
                     /translation="MATLSFRAATKTYSPGNIQVIHGIDLDIADGEFIVIVGPSGCGK
                     STLMRMVAGLESITSGEILIGDAVVNKLEPAERDIAMVFQNYALYPHMSVFDNMAYGL
                     KIRKLAKQDIRQRVDKAAEILEIAHLLDRRPRQLSGGQRQRVAMGRAIVREPKVFLFD
                     EPLSNLDAKLRVQMRLEIQKLHRRLGTTSLYVTHDQVEAMTLAQRMMVMNKGVAEQIG
                     TPSQVFEKPASTFVASFIGSPPMNLIPVQIDGMRQVRDMAQAPLQIDPAQVPASLVNR
                     HVIMGLRPEHMTLHAAGVPIEIEMIEILGSEQLIHGRHGDTQIVLRCAVGLTKERQIH
                     IGDTVQTGAASPQMFHWFDPDTRQRIDA"
     gene            complement(3944..>4601)
                     /locus_tag="VIR04_03100"
     CDS             complement(3944..>4601)
                     /locus_tag="VIR04_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459770.1"
                     /translation="AAFIADTIKPKRVAVLHDKQSYGQGIATAVKNALEKKGIDVALF
                     EGINAGDSDYSAVITKLKSADVDFVYYGGYHPEMGLLLRQGSEQGLKARFMGPEGVGN
                     ADISGIAGPAVEGMLLTFPADFTKNPENASIVKAFKDKKRDPGGAFQLTAFSATMAIV
                     DGIKGANADDPVKVAAYLHAHTVSTPIGKISWNKQGDLTNFKFDIFTWHKDGTKTVYK
                     "
BASE COUNT         1019 a         1269 c         1202 g         1111 t
ORIGIN      
        1 cttggcgccg tggccagcta ctattcctcg cctgacggca aactggtgtc gatgccattc
       61 aacagttcca cccctgtttt ctactacaac atggacgcgt tcaaaaaggc cggactcgat
      121 cctcagaagc cgcccaaaac gtgggacgac gtagcgcagg ccgctacaaa gctcagagcg
      181 gcgggactcg aatgcggcta cagcacgggc tggccatcgt gggtgcaact tgaaactttt
      241 tccgcctggc ataatgcgcc gtatgcgtct aaaaataatg gttttggcgg ccttgatgcg
      301 cgcctggaag tcaacaagcc cctgttcgtg cgccacatga cgtttttggc aaaaatggcg
      361 aaagaaggca cgtttacata cggcggtcgc ggcgatgccg ccaatgcgct cttcatatca
      421 ggcaaatgcg gcatgttcac cggctcaagc ggcatgcgcg ccagcgtcaa gaaaaacgcg
      481 aattttgaat tcggcacatc gccgcttccc tactatgccg atgtcaaggg cgctccgcaa
      541 aatacaatca tcggaggagc ttcgctgtgg gtgttcgcga aaaaatcccc cgaagtatac
      601 aaaggcatta ccaaattctt taaatttctt gccagtccgg agcaagcggc cgcctggcat
      661 cagggcaccg ggtatgtgcc agtcaccaaa gccgggtacg agctaacgaa gaaatccggc
      721 ttctacgaca agaaccccgg cacggatgtg gcagtcaagg aactggacgc cactacaact
      781 aaaaactcac gcggtgttcg tctgggctat ctgccccaaa ttcgtgacat cgaggacgcg
      841 gacatggagc agattttcgc tggcaaggtc actccgaaac aagggctgga caacatggtt
      901 aaacgcggcg atgaactcct ggcgcgattc cagaaaagtg ttaaataact gaatctgagc
      961 ctgtgcagag gatgacccct ctgcaggccc cgatgcgcca atatcagcac ggctggatat
     1021 aaatacggtt atctatggaa aaacgcgtcg ttttcaacca caaaactttg ccgtatctgc
     1081 ttctggcgcc gcaattggcc atcacagtca tttttttctt ctggccggcg ggccaggcaa
     1141 tgtggcagtc ctttcgaatt gaagacccgt tcggtttgtc atcccagttt gcgggcctga
     1201 gcaacttcat tgagctcttt tcgaattcgg attacatcaa ttcgttcaaa gtgacggccg
     1261 tattttccgt gctggtcgcc gtattgggcc tgagcatttc tctggtgctg gcccttatgg
     1321 ctgatcgggt cgtcaaaggc gccaagctgt acaagaccct gctggtttgg ccctacgccg
     1381 ttgcaacggc cgtcgccggc gtgttgtggc tatttatgtt ttcgccgtcg gtgggagtta
     1441 ttgcagtata cctggccaat cttggattta gctggaaccc tcgtctgaac tccaccgacg
     1501 ccatgacgct ggtcgtgctg gcttcggtgt ggaagcagat ttcatacaac tttctttttt
     1561 tcctggccgg actgcagtcc ataccgcgct cgctcatcga ggccgctgcc attgacggcg
     1621 caagtcccat gcggcgcacg atgaccatta ttttgccgct tttgacgccc accacctttt
     1681 ttctgctggt ggtgaatatc atttacgcat tcttcgacac gtttgccgtc atcgacatca
     1741 tgacccaggg aggtcccggc aacacgacgg ccatcctggt ttatcgtgtc tataacactg
     1801 gctttctggg cctggacatc ggctcatcag cagctcaatc cgttattttg atggccattg
     1861 tcgtcgcgct tacgatcgtg caattccgct acgtcgaacg caaagtcaac tactagcctc
     1921 aagcttaata atgcttcacg cccatgattg aacgccgccc ctacctggat ttcttctcgc
     1981 atctggtttt attgattggc gtggctgtca tcgcgtttcc gctatacctc acattcattg
     2041 cgtcgaccca aaccgcgcaa gaagtcgcac aagcgcccat gtccttgttg ccgggcggac
     2101 attttctgga aaattatcaa acggcgcttt tcggcggagt gggcaatacc gcgcctgtgg
     2161 cgcggatgat gtgggtcagc accgtgatgg cgctggtgat tgccatagga aaaatttcga
     2221 tttccatgct atcggccttc gccgtcgtat attttcattt ccggttcaga atgtttttct
     2281 tctggatgat tttcatcacc cttatgctgc cggtcgaggt gcgcattacg ccgacctaca
     2341 aggtggtggc cgatctcggc atgctcaata cctatgcggg cctgaccctg cctctgattg
     2401 catcggccac cgcaacattt ctgtttcgtc agtttttcct gaccgtaccc gacgagctca
     2461 tcgaggcggc gcgtattgat ggcgcggggc ccatgcggtt ttttaaagat attctgcttc
     2521 ctttgtccag cacgacgatc gccgccctgt ttgtcattca gtttatctac ggctggaacc
     2581 agtacttgtg gccgctcatc attacaacca aggaaaatat gtatcctgtc gtcatcggca
     2641 ttaaacgcat gatcgccggc ggcgatagcg tcacagaatg gaatctggtc atggcgacgg
     2701 cactgcttgc catgattcct ccggcggccg tggtcatctt catgcaaaag tggtttgtca
     2761 aaggtctggt cgatgcagaa aaataacgcg gtttacgtaa acaggcaatc atggctactt
     2821 taagctttcg ggccgccacc aaaacctatt caccgggcaa tatacaggtt atacatggca
     2881 tcgatctgga catagccgat ggcgaattta ttgttatcgt gggaccatcg ggttgcggaa
     2941 aatccacctt gatgcgcatg gttgccggac tcgagagcat caccagcggc gaaatactga
     3001 ttggcgacgc cgttgtaaac aagctggaac cggcagaacg cgacattgcc atggtgttcc
     3061 agaactatgc gctctatccg catatgtccg tattcgacaa tatggcgtat ggattgaaaa
     3121 tccgcaaact tgccaaacag gacattcgcc agcgcgtgga caaagcggcg gaaatcctgg
     3181 agatcgccca tttgcttgac cggcgcccac gtcaactttc gggcggccag cgccagcgag
     3241 tggcaatggg gcgcgcgatc gtgcgcgaac ccaaggtatt tttattcgac gagccattat
     3301 ccaatctgga tgccaagctg cgcgtgcaga tgcggctgga aattcagaaa ttgcatcgcc
     3361 gcctgggaac aaccagtctg tatgttacgc acgaccaggt cgaagccatg acgctggcac
     3421 aacgcatgat ggtcatgaac aaaggcgtgg cggaacagat cggcacgcca tcacaagtat
     3481 ttgagaaacc cgccagcacc ttcgtcgcaa gttttatcgg ttcgccgcca atgaacctga
     3541 ttccggtgca aatcgacggc atgcgtcagg tcagggatat ggcgcaagcg ccgcttcaaa
     3601 tcgaccccgc ccaagtgccc gcgtcgttgg tcaatcgcca cgtcatcatg ggactgcggc
     3661 ccgaacacat gacgctgcat gcggcggggg tgccgatcga aattgaaatg attgaaatcc
     3721 ttggatcaga gcaactcatc catggccgcc acggcgacac gcaaattgta ttgcgttgcg
     3781 ccgtgggttt gacaaaagag cgccagattc acattggcga cacagtgcag actggagccg
     3841 ccagcccgca gatgtttcac tggtttgatc ccgacaccag gcagcgcatt gatgcgtgaa
     3901 cctgcgtcac caatgcgcca ggcgcgcgct tgaattcatg cgcctatttg taaacggttt
     3961 ttgtaccgtc tttgtgccat gtaaatatat cgaacttgaa gttggtcaga tcgccctgct
     4021 tgttccatga gattttgcca ataggcgtgc tgaccgtgtg cgcatgcaag taggcggcca
     4081 ccttgacggg gtcatccgca ttagcgccct tgatgccatc aacaatggcc atcgtcgccg
     4141 agaaagccgt taactggaag gcgccgcccg gatcgcgttt tttatcctta aacgctttaa
     4201 cgatactggc gttttcggga ttttttgtga agtcggccgg gaatgtgagc aacatgccct
     4261 cgacagcggg gcccgcaata ccacttatgt cagcgttgcc tacgccttca ggccccatga
     4321 accgggcctt caggccttgt tcggaaccct gacgcaataa cagccccatt tcaggatggt
     4381 aaccgccgta ataaacgaaa tcgacatcgg cgcttttgag cttggtgata accgctgaat
     4441 aatcgctgtc gcctgcgttg atcccctcga aaagcgccac gtcaataccc tttttttcaa
     4501 gggcattttt taccgccgtg gcaataccct ggccatacga ttgcttatcg tgcaatacgg
     4561 cgacgcgttt cggtttgatc gtatctgcga taaacgcggc g
//
LOCUS       DATCXU010000140         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_335468,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000140 DATCXU010000000
VERSION     DATCXU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335468"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2232
                     /locus_tag="VIR04_03105"
     CDS             <1..>2232
                     /locus_tag="VIR04_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY9459771.1"
                     /translation="VAAVSREQAQRARDTDPYWLAQRKGLLLLERGNMASAAPLLEKA
                     MTRRKNDPELLGGLGILRMRQSRQMEALPLFQRAAQLDASGREKWEGLAATARFWGTL
                     NQSGDAAKRGDMAKAERYARAAIAMQPDNAYARKVLVDTLLAQKKWKQAEPLLRRLLA
                     QRTVDIDVLRDMGTLLRESGRASEIEHLMNGLRSRFAGKGLQAFQQLRADQLSANADL
                     LLKQDKVGQALEKLELSLRDAPDAAWTRYTLARTYLRLGLPKLGQSVMDEGFALTKAP
                     DMSYATALYRNGQDDVPGAMIAMARIAQAQRTEGMRALALNLQAQRLLKEARGQFAKN
                     DLAAGKKSLDRAAALAVNDPYMLASLGSEWIAQGDSEHGLGLLRNWIREHPGKTDADI
                     LLRYGDLLASAGHEDQLKNWLTNVAKVPDLTDAQRRRLEDQSLRRILRATDTALSDED
                     YERAEVLLHQVDAAGRKDQRWSLELVDLRREQGRYAEARAVLASLLAKKPDDFEARLA
                     LARVLERSGDRKEALAMVRGVVGEAPPEDIDTRLSAARRLVALRRPREAAVLVSDLRD
                     QFGERADVTVQQGRILESLGQYDLAKVQYQAALKQEMEHGVKGTAAQEALGDLELRRQ
                     PLIETALTPSYNVGTPGVSLLHAVAAPVRVQVPQGYDGHWFFHADSVKLDAGTLDDTA
                     DAFSWQSFGQFAAFPESAHGSFDTKAAGVALGAGFEADNWRADLGTTPLGFPVKNLVG
                     GLRV"
BASE COUNT          465 a          575 c          732 g          460 t
ORIGIN      
        1 gtggctgccg tttcccggga acaagcgcag cgcgcgcgtg acactgatcc gtattggctg
       61 gctcagcgca agggtttgct tcttttggaa cggggcaata tggccagcgc cgcgcctctg
      121 ctcgaaaaag cgatgaccag acgcaaaaac gaccctgaac tgcttggcgg cctgggcata
      181 ttgcggatgc gccaaagccg ccagatggaa gcgctgcccc tgtttcagcg cgctgcgcaa
      241 ctagatgcct ccggacgtga aaaatgggaa ggtttggcgg cgacggctcg tttttggggc
      301 actttgaatc aaagcggaga cgcggccaag cgtggcgata tggctaaagc cgagcgctat
      361 gcccgtgcgg cgatcgcgat gcagcccgat aatgcgtatg ctcgcaaggt tctggttgat
      421 acattgctgg cgcaaaaaaa atggaaacag gctgagccct tgttgcgccg actgctcgcg
      481 caacgtacgg tagacattga cgtattgcgc gacatgggaa ccttgttgcg cgaatcgggg
      541 cgagcgagcg aaatcgaaca cctcatgaat ggtctgagga gccgtttcgc tggcaaaggc
      601 ctgcaggcat ttcagcagtt gcgcgccgat cagcttagcg caaacgccga tcttttattg
      661 aagcaagaca aggtgggtca ggctctggaa aaactggaac tgtcgttgcg cgatgctccg
      721 gacgcggcat ggacgcgtta taccttggcg cgtacctacc ttcgtctggg cctgccgaaa
      781 ttgggacagt ctgtcatgga tgaaggcttt gccctcacca aagcgcccga catgagctac
      841 gccacggcgc tttaccgcaa tggccaggat gacgtgccgg gcgcgatgat tgccatggcg
      901 cgaattgcgc aagcacagcg aactgaaggc atgcgcgccc tggcgctgaa tttgcaggcg
      961 cagcgcttgt taaaagaagc gcgtggtcag tttgcgaaga acgaccttgc agccggtaaa
     1021 aaaagcctgg atcgggccgc cgcgttggcg gtgaacgacc cctatatgct tgcttcactg
     1081 ggctccgagt ggattgcgca aggcgattcg gaacatggcc ttggtttgct gaggaactgg
     1141 atacgcgagc atccgggcaa gacggacgcg gatatattgt tgcgttatgg agatttactg
     1201 gccagcgccg gtcatgaaga tcaattgaaa aattggttaa ccaatgttgc caaagttccc
     1261 gatttgaccg atgctcagcg gcgtcgcctg gaagatcaaa gcttgcggcg gattttgcga
     1321 gcgacagata cggcattgtc cgatgaagac tatgaacggg ccgaagtgct gttgcatcaa
     1381 gtcgacgcgg ccggccggaa ggatcagcga tggtcgctgg aattggttga cttgcgccgc
     1441 gaacagggcc gctatgcgga agcgcgcgcg gtattggctt cgttgttggc aaagaaaccc
     1501 gatgattttg aagcgcggct ggcgctagcc cgagttcttg agcgtagcgg cgatcgtaaa
     1561 gaggcgcttg cgatggtgcg aggcgttgtc ggggaagcgc cccccgaaga tatcgacacg
     1621 cgtctgtcgg cggcgcgccg gctggtggcg ttgcgcaggc ctcgagaagc ggccgtgttg
     1681 gtcagtgatc tacgtgatca attcggcgag cgtgccgacg tgactgtgca acaaggccgg
     1741 atactggagt cactgggtca atatgatttg gccaaggttc agtaccaggc ggcgctgaaa
     1801 caagaaatgg agcatggcgt taaggggact gcggctcaag aagctttggg cgatctggaa
     1861 ttgcgccgcc agccgctgat tgagaccgcc ctgacacctt cctataacgt tggcacgccg
     1921 ggcgtttcat tgctgcatgc tgttgcggcg cccgtgcgcg ttcaggtacc gcagggttac
     1981 gatggtcact ggttttttca tgcggactcg gtgaaactgg atgcaggcac gctggacgat
     2041 acggcagatg ccttttcctg gcagtcattc gggcagtttg ccgctttccc ggaaagcgct
     2101 cacggatcgt tcgatacgaa agcagcggga gtagcgctgg gcgcggggtt cgaagccgac
     2161 aactggcgtg ccgacctcgg gacaactccc cttggtttcc ctgtgaagaa cctggtgggc
     2221 ggtttgcggg tc
//
LOCUS       DATCXU010000141         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_597509,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000141 DATCXU010000000
VERSION     DATCXU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597509"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="pip"
                     /locus_tag="VIR04_03110"
     CDS             <1..863
                     /gene="pip"
                     /locus_tag="VIR04_03110"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY9459772.1"
                     /translation="CGNPAGKAAIFLHGGPGSGCAPTHRRLFDPQRYNVLLFDQRGCG
                     RSTPHASLENNTTWHLVSDIERLRGEILNAEKCQIFGGSWGSTLALAYAQTYPERVSE
                     LIVRGIFTIRRAELQWFYQEGASLLFPDYWEDFLAPIPNDERDDLMTAYHKRLTSSDL
                     AVQLKAAHAWTQWESRTITLLPSATYLKSHSDDASALAFARIENHYFVNKGFMQEGQL
                     LRNANKLHAIPGVIVQGRYDVCTPAKTAWDLHKVWPQAEFHLIDDAGHAFDQPGNLAQ
                     LLAATDKFSR"
     gene            complement(860..2266)
                     /gene="ffh"
                     /locus_tag="VIR04_03115"
     CDS             complement(860..2266)
                     /gene="ffh"
                     /locus_tag="VIR04_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY9459773.1"
                     /translation="MLDNLTHRLSRVVKTMRGQARLTEANTQDMLREVRMALLEADVA
                     LPVVRDFIARVKESALGQEVVGSLNPGQALVGVVHKQLTALMGGDLEGGAGELSLAAQ
                     PPAVILMAGLQGAGKTTTTGKLAKWLAEGGHVQNGHKSGKKKVLVVSADVYRPAAIEQ
                     LKTVAAQVKVDFLPSDTTQKPEDIARVALDHAKRYHYDVLIVDTAGRLGIDLAMMQEI
                     RALYDLLTPIETLFVVDAMQGQDAVNVAKAFADALPLTGIVLTKLDGDARGGAALSVR
                     HITGKPLKFVGVSEKLDGLQAFYPERMAERILGMGDIVSLVEQAQQNIDVAEAQKLAK
                     KIKSGRRFDLNDFRDQLKQVKNLGDMNSVLEKLPAQFSQAASQLQSGQAEKQLRRTEG
                     ILCSMTAAERAKPELLKASRKRRIAAGAGVPVQEVNRMLAQFDQMQGMMKQMKKGGIA
                     KMMRAMGGMKGMGKGGFR"
BASE COUNT          516 a          797 c          648 g          548 t
ORIGIN      
        1 tatgcggcaa cccggccggt aaagccgcca tttttttgca cggaggcccc ggtagcggct
       61 gcgcacccac gcaccggagg cttttcgacc cgcaacgcta caacgtactg ttattcgacc
      121 agcgcggctg cggccgctcg actccccacg ccagccttga aaataatacg acatggcacc
      181 ttgtttcaga catcgagcgc ttgcgcggcg agatcctgaa tgccgaaaaa tgccagattt
      241 ttggcggatc atggggttcg accctggcgt tggcatacgc ccaaacctat cccgagcgcg
      301 tcagcgaact gattgtgcgc ggcatcttca ccattcggcg cgccgaattg caatggttct
      361 accaggaagg cgcctcgctg ctgtttcctg actactggga agattttttg gcccccatcc
      421 caaacgatga acgcgacgat ttgatgaccg cgtaccacaa gcgcctgaca tccagcgatc
      481 tggcggtgca actgaaagcc gcccatgcct ggactcagtg ggaaagccgc accattacct
      541 tgctgcccag cgcgacatac ctgaagtcac actcggatga cgcctcggcg ctggctttcg
      601 cgcgaattga aaaccactat ttcgtcaaca agggttttat gcaagaaggg caattgttgc
      661 gcaacgcgaa taagctgcac gccattcccg gcgtaatcgt tcaaggccgc tacgatgtgt
      721 gcacacccgc gaagacggca tgggaccttc acaaagtctg gccgcaagcg gaattccatc
      781 tgatcgacga tgcgggtcat gccttcgatc aacccggaaa cctggcccaa ttgctggcgg
      841 caacggacaa gttcagccgt taacgaaagc cgcctttgcc catgcctttc atgccgccca
      901 tggcgcgcat catttttgcg atcccgcctt ttttcatctg cttcatcatg ccttgcattt
      961 gatcaaactg ggccagcatg cgattcacct cctgcacggg aacgcccgcg ccggcggcaa
     1021 tgcgccgctt gcgggacgct ttgagcagtt ccggcttggc acgttcggcg gcggtcatcg
     1081 agcataaaat gccttcagtc cggcgcaatt gcttttctgc ctgcccgctc tgcaactggc
     1141 tggccgcttg cgaaaactgg gctgggagtt tttccagcac cgaattcata tcgcccaagt
     1201 ttttaacctg tttcaactga tcgcgaaaat cattcaaatc gaaacgcctg cccgatttga
     1261 tcttcttggc cagtttctgc gcttcggcca catcaatgtt ttgctgggct tgctcgacca
     1321 gtgaaacgat gtcgcccatg cccaatatgc gttcggccat gcgttccggg taaaacgcct
     1381 gaaggccatc gagcttttcc gacacaccaa caaatttcaa tggcttgccg gtaatatggc
     1441 gcaccgacaa ggccgcgccg ccacgcgcgt cgccgtcaag cttggtcagc acgataccag
     1501 tcagtggcaa cgcgtcggca aaggccttgg ccacattgac ggcatcctgg ccctgcatgg
     1561 cgtcaaccac gaacaaggtc tctatcggcg tcagcaaatc gtataaggcg cgaatttcct
     1621 gcatcatggc caggtctatc cccagccggc ccgccgtatc cacgatcagc acatcgtagt
     1681 gatagcgctt ggcatgatcc agcgccacgc gcgcaatgtc ctcgggtttc tgcgttgtgt
     1741 cggaaggcag aaaatcaacc ttcacttgcg cggccaccgt tttaagctgt tctattgccg
     1801 ccgggcgata cacgtcggcg ctgaccacca gcaccttttt cttgcccgac ttgtggccat
     1861 tttgaacatg gccgccttca gcgagccatt tcgccagctt gcccgtcgtc gtggttttac
     1921 ccgcaccctg caagccagcc atcaatatca ccgccggagg ctgtgccgcc aaagacagct
     1981 cacccgcgcc gccctcgagg tcgccgccca tcaatgccgt gagctgcttg tgcaccacgc
     2041 ccaccagcgc ctggccgggg ttcaggctgc ccacgacctc ctggcccagc gcactctctt
     2101 ttacccgggc gataaagtcg cgcaccaccg gcaaggccac atcggcctcg agcagcgcca
     2161 tgcgcacttc gcgcaacata tcctgagtat tggcttctgt tagccgcgcc tgaccgcgca
     2221 ttgttttcac gacgcgggac aggcggtgag tcagattatc gagcatactg atagattcgc
     2281 ttaaacgtgt aggattaagg gataaggtct aaaatgcctt acttgggcat tatgccccac
     2341 cttataaaat cagaacttca gggactatgt ctgcaggcat tgtatttcac tcgctcgccg
     2401 cgattgccta cgcaatttta gccattgcca tctggcgccc gctctcgcga ggcggcgcac
     2461 aagccaaaat tggcgcaacc ggccgaacgt gcctgcttgg cgccgttat
//
LOCUS       DATCXU010000142         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_491509,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000142 DATCXU010000000
VERSION     DATCXU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491509"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..990)
                     /gene="rfaE1"
                     /locus_tag="VIR04_03120"
     CDS             complement(61..990)
                     /gene="rfaE1"
                     /locus_tag="VIR04_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY9459774.1"
                     /translation="MSPFPLQAVGNVNVLVVGDVMLDRYWFGEVDRISPEAPVPVVRV
                     ARREDRLGGAANVARNIVALGAHATLVGIVGTDEAGQQVKALTRQAGINENLIGDADL
                     PTTLKMRVLGRQQQLLRVDFEQTPAGASLTRLKSDVLALIAQHDMVVLSDYAKGALEQ
                     VDALIHAARDAGVPVLVDPKGHLYRRYRGATLLTPNRIEMQEAVGRWQTEDELEQRAQ
                     LLRAELELEALLVTRSERGMTLFTKKGRQHTDAQAHEVFDVSGAGDTVLAALAVTRAA
                     GLDWFDAVLWANRAGGIVVGKLGTSVVTAEELK"
     gene            complement(987..2204)
                     /gene="lapB"
                     /locus_tag="VIR04_03125"
     CDS             complement(987..2204)
                     /gene="lapB"
                     /locus_tag="VIR04_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY9459775.1"
                     /translation="MDFEPWWLVFLPLLFALGWIAARVDFRQMLSETRTLPDSYFKGL
                     NFLLNEEPDRAIDAFIEVVKLDPETTELHFALGSLFRRRGEVERAIRVHQSLLARADL
                     PQASREHAQHELAQDFLKAGMLDRAETAFQTLQDSRFALQAVRALIRIYESEHDWPRA
                     IEAVKRLRGLIDEPVPQLVHYQCELAQMALAAKPPDIKAANEALDAADNAAHALRGQE
                     RGQASEARIAMLRAALARADHNSERERVYLASVLAIAPEYAGLVAADILESYRQVGLQ
                     AEGLQLLRNHYMRHPSLDIFNVVFREMRAQEGYAMAWNFAREALREHPSLLGLDRLLE
                     AELASSGASTAFSSASGADLSLLRSLIHKHTQRLDRYACRVCGFEARHFYWQCPGCNS
                     WETYQPRRLEELK"
     gene            complement(2232..2555)
                     /locus_tag="VIR04_03130"
     CDS             complement(2232..2555)
                     /locus_tag="VIR04_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY9459776.1"
                     /translation="MRYLVWILRLLVFVLILLFALKNTGTVDVNFYADHVATGVPLIV
                     VMLVAFVLGVVFGLLLTVPSVMRRRREVKKLKRDMIRLQDRLKFPGAPSNAPAPETMA
                     PLAPF"
     gene            complement(2718..3074)
                     /locus_tag="VIR04_03135"
     CDS             complement(2718..3074)
                     /locus_tag="VIR04_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY9459777.1"
                     /translation="MTKSELIAILAGRYPQLAIRDMDYAVKTVLDAMAQALADGQRIE
                     IRGFGSFSLSRRLPRIGRNPKSGEQVLVPGKRVPHFKSGKELRERVDAAFDEKVRAQD
                     ESAFKLGQFNDRLMQG"
     gene            complement(3080..>3775)
                     /locus_tag="VIR04_03140"
     CDS             complement(3080..>3775)
                     /locus_tag="VIR04_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY9459778.1"
                     /translation="MVLEIDEDRRRISLGMKQCRANPWEEFSINFKRGDKVRGAIKSI
                     TDFGVFVGLPGGIDGLVHLSDLSWTETGEEAVRNFKKGDEIDAVVLGIDTDKERISLG
                     IKQLEGDPFNNFVATYDKGAVVPGTIKQVEPKGAVVTLSVDVDGYLRASEISTGRVED
                     ATTVLKVGESVETMIVNIDRKSRSIQLSIKARDTADTAETIQRMTEASASSGTTNLGA
                     LLKAKLDQQRDDG"
BASE COUNT          854 a         1186 c          980 g          755 t
ORIGIN      
        1 ggcggttcag gccgcgcacc aggttgctgc cgataaagcc ggcgcctccg gtcacaataa
       61 tcatttcaat tcctccgctg tgacaactga cgttcccaat ttgccaacaa caataccgcc
      121 tgcgcggttg gcccataata ccgcatcgaa ccagtccagc ccggcggcac gcgtaaccgc
      181 cagcgccgca agaaccgtat cgcccgcgcc cgacacatcg aatacctcgt gggcctgggc
      241 atcggtatgc tggcgcccct ttttggtaaa taacgtcatg cctcgttcag aacgagtgac
      301 cagaagcgcc tcgagttcaa gctcggcacg cagcaactga gcccgctgct caagctcatc
      361 ttcggtttgc cagcggccca ccgcctcttg catttcgatc cgattgggag tcagaagcgt
      421 tgcgccgcga taacgccggt acagatgccc tttgggatcc accaaaacgg gaacaccagc
      481 gtcgcgcgca gcatgaatca gcgcatcgac ctgttccaga gcacccttgg cgtaatcgga
      541 caacacgacc atatcgtgtt gcgctatcag tgcaaggaca tcggacttca ggcgcgtcag
      601 gctggcgccg gccggagtct gctcaaaatc aacacgaagc aactgctgct gacggcccaa
      661 aacacgcatc ttcagcgtcg ttggcaaatc ggcgtcccca atcaggttct cgttaatgcc
      721 tgcttgcctc gtcagcgcct ttacctgctg ccccgcctcg tcggtgccga caattcccac
      781 cagtgtcgcg tgcgcgccaa gcgccacaat attgcgcgcc acgttggccg caccgcccaa
      841 gcgatcttcc cgacgcgcca cccgcacgac cggtacgggc gcttccggcg agatgcggtc
      901 cacttcccca aaccagtacc ggtcaagcat gacatcgccc accaccagca cattgacatt
      961 gccaacagcc tgcaaaggaa acgggctcat ttcagttcct cgaggcggcg cggctgatag
     1021 gtctcccacg aattgcaccc cggacactgc caatagaaat gacgcgcttc aaaaccgcat
     1081 acccggcaag cgtaacgatc caggcgctgc gtatgcttat gaatgagcga acgcagcaag
     1141 cttagatcgg cgccagacgc cgagctgaac gcggtcgagg cgccgctgct ggccagctct
     1201 gcttcgagca agcgatccag ccccagcaag gatggatgct cgcgcagagc ctcgcgggcg
     1261 aaattccagg ccattgcata gccttcctga gctctcatct cgcgaaacac cacattgaat
     1321 atatccagtg acgggtgccg catatagtga ttgcgcaaca attgcaggcc ctccgcctgc
     1381 aatccaacct gtcgatagct ctcgaggata tccgccgcca caagtccggc gtattcgggg
     1441 gcaatcgcaa gcaccgacgc caggtatacg cgctcgcgct ccgagttgtg atcagccctg
     1501 gccaatgccg cccgcagcat tgcaatacgg gcttcggatg cctgtcctcg ctcctggccg
     1561 cgcaaggcgt gcgccgcgtt atccgctgca tcgagcgcct cgttcgccgc ctttatgtca
     1621 ggaggcttgg cggccaaagc catttgcgcc agttcgcatt gataatgcac cagttgtggc
     1681 acaggttcgt cgataagccc acgcagacgc ttcacagcct caatggcgcg cggccaatca
     1741 tgctcagact cgtaaatacg aattaaagcg cgcaccgcct gtagcgcgaa tcgactgtct
     1801 tgcaaagttt gaaaagcggt ttctgcccga tccagcatgc ccgctttgag gaaatcctgg
     1861 gcaagctcat gctgagcatg ctcgcggcta gcctgcggca aatcggcgcg cgccagcaag
     1921 ctttgatgca cgcgtatggc gcgttcgacc tcgcctcgac gacgaaataa actgcccagc
     1981 gcaaagtgca gctcggtggt ttcaggatcc agcttgacca cctcgataaa ggcgtcgatc
     2041 gcccggtcgg gttcctcgtt gaggaggaaa ttgagccctt taaaataaga gtctggcaaa
     2101 gtccgagtct cggacagcat ttgccgaaaa tcaacgcgcg cagcgatcca gccaagcgcg
     2161 aacagcaagg gcaaaaatac gagccaccac ggttcaaaat ccactctggt cgacctttac
     2221 tttacgttac gttaaaacgg cgccaaaggc gccatggtct cgggcgcggg ggcattgctt
     2281 ggggcacctg ggaatttaag ccgatcttgg aggcggatca tatcgcgctt gagttttttt
     2341 acttcgcggc gacgccgcat tactgagggg acggtcagca acaagccaaa cacaacgccc
     2401 aatacaaacg ccaccagcat cactacaatc agcggcacgc ccgtggcaac atggtccgcg
     2461 taaaaattga cgtcaacggt tccggtattt tttaatgcaa atagcaggat cagcacgaaa
     2521 acgagtaatc gtaatatcca gaccaggtaa cgcatgatga agactccaat aaaccgggaa
     2581 ttccacaatt gtaaggggaa tcaccacgcc agccgcgcgc ttgcgcacaa ctcaatcaaa
     2641 caggccctcg ttttgagggc ctatctgaac tgaacgccaa caagcttcat tggcgggcac
     2701 ggtatgaccg atccaggcta accctgcatc aggcgatcgt taaactgtcc cagtttgaat
     2761 gcggattcat cctgagcccg taccttttca tcaaaggccg catcaaccct ttcgcgcaat
     2821 tccttccccg atttgaaatg aggcacccgc ttgcccggca ccagcacctg ctcacccgac
     2881 tttgggttcc ggccaatgcg aggcaaacgc cgcgataatg aaaaactgcc aaaaccgcgg
     2941 atttcgatgc gctgaccatc ggccaaagcc tgggccatgg catcaagaac cgtcttgacg
     3001 gcatagtcca tatcgcgtat cgcaagttgc gggtagcgac cagccagaat cgctatcagt
     3061 tcagacttgg tcacaccaat taaccgtcgt cacgttgctg gtcgagcttg gctttaagca
     3121 gagcgcccag attggtcgtg cctgacgaag cactggcttc ggtcatgcgc tggattgttt
     3181 ccgccgtatc ggcggtatcg cgcgccttga tcgacaattg aatcgaccgc gacttgcggt
     3241 caatgttcac gatcatcgtt tcgacgcttt caccaacctt tagcacggtc gtggcatctt
     3301 ccacgcggcc cgtcgaaatt tcggatgcgc gcaaataacc gtcaacgtcg accgacaaag
     3361 taaccactgc gcctttcggc tcgacctgtt tgatcgttcc gggaaccaca gcccccttat
     3421 cgtaagtagc cacgaagtta ttgaacggat cgccttcgag ctgtttgatg cccagcgaga
     3481 tgcgttcctt gtcggtatcg atccccagaa ccactgcgtc gatttcgtcg cccttcttga
     3541 agttgcgtac ggcttcttcg cccgtttcgg tccaggacag atcggacaag tggaccagtc
     3601 cgtcgatccc gccaggcagg ccgacaaaca caccgaagtc ggtaatcgac ttgatcgcgc
     3661 cgcggacctt gtcgccgcgc ttgaagttga tggagaattc ttcccaagga ttcgcacggc
     3721 actgcttcat gcccagcgaa atacggcggc ggtcttcgtc gatttccaga accat
//
LOCUS       DATCXU010000143         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_900304,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000143 DATCXU010000000
VERSION     DATCXU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900304"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIR04_03145"
     CDS             complement(<1..468)
                     /locus_tag="VIR04_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY9459779.1"
                     /translation="MSSLRPLMRHKIQIVIFSSLLLLGACGDKDQQHSGEIKVPVSVV
                     TVEPARTEIFVDLPGRVEAIREAQIRARVTGIVSQTNFKQGSDVVRGQLLFTIDPTPY
                     RAVRDQAAAQLKRAQADAHGASLLAQRYAKLIRAKAISSQDYDNARAAAAQAEA"
     gene            complement(635..1090)
                     /locus_tag="VIR04_03150"
                     /pseudo
     CDS             complement(635..1090)
                     /locus_tag="VIR04_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            complement(1207..1992)
                     /locus_tag="VIR04_03155"
     CDS             complement(1207..1992)
                     /locus_tag="VIR04_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY9459780.1"
                     /translation="MDERVNPSLPSPADEPTISELDGIRYLHFSTPWVQGAMRIKNPS
                     ELVLAYARQMMAWLLFLAPGKESTIGILGLGAGSLVRFALKQTRSSVVTVEWNARVIA
                     ACRAYFRLSKSARSTVDQCDAANWVRQSRNAGRCCALMVDIYDAHARGPVHESLEFYQ
                     GCYRSLSDSGVMTVNLFGDHESFPRNLANIRAVFDGGVLELPEIDVGNRVILAFKGPL
                     LEIGALQFMDRARLVESRYGLPAVRWAKSLLRQRDFRDRSRKV"
     gene            complement(2014..3195)
                     /locus_tag="VIR04_03160"
     CDS             complement(2014..3195)
                     /locus_tag="VIR04_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEY9459781.1"
                     /translation="MEDVVVVGACRSAVGDFGGALKNMAPCDLGSTVIRAVLERTGIP
                     GSDVGHVVVGHVINTEPRDMYLSRVAAMNAGIGQSTPAFNVNRLCGSGLQAIVSAAQA
                     LWLGDTDIAIGAGAESMSRAPYIAPAQRWGARMGDSVLLDMMTGALSDPFDKEHMGIT
                     AENVARKYGVSRAEQDALALSSHQRAAAAIKAGYFKEQIVPIVQKTRKGEVVFDVDEH
                     VRFDATAADFSALKPVFMKENGTVTAGNASGLNDGAGAVLLMTQKAARDRGIKPMARL
                     VAYGHAGVDPKYMGMGPVPATRMALRKSGLDIGQFDVIEANEAFAAQACAVSKELGFD
                     PEKVNPNGSGISLGHPIGATGAILVVKALHELNRIQGRYALVTMCIGGGQGIAAIFER
                     I"
     gene            3318..4097
                     /gene="xth"
                     /locus_tag="VIR04_03165"
     CDS             3318..4097
                     /gene="xth"
                     /locus_tag="VIR04_03165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY9459782.1"
                     /translation="MKLATWNINSLKVRLPQVLEWLKNHPVDALCLQELKLPDDRFPS
                     EAFAAAGYQACWAGQKTYNGVAIISRDPARDVQRDLPGLDDPQRRIIAATLPGPAGDI
                     RVISAYCPNGQTVGSDKYHYKLKWFGVLREWLREELSHYPHLAVLGDYNVAPADEDVH
                     DPAKWVGEVLVSEPERMAFRSLLDLGLTDAFREFEQEKKSFSWWDYRRLAFQRNAGLR
                     IDHVLLSDALRMHCSACVIDKEPRRNAQPSDHAPVIATLTT"
     gene            4139..4780
                     /gene="gmk"
                     /locus_tag="VIR04_03170"
     CDS             4139..4780
                     /gene="gmk"
                     /locus_tag="VIR04_03170"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY9459783.1"
                     /translation="MSTNPAHANIFMVVAPSGAGKSSLVNALLLQDASLFLSISCTTR
                     EPRPGEQAGKHYRFVAKEEFFAMRQNDALLEWAEVHGHFYGTPRDNIDRAMQQGRDAL
                     LEIDWQGARQVKKYFPHAIGIFILPPSIEALEKRLYSRGQDAPQVISRRLLAAGGEMS
                     HASECQYVIINQEFSVASNQLAQIVAATRLRYETQAVRNAALFSQLGISTTTA"
     gene            4813..>4983
                     /gene="rpoZ"
                     /locus_tag="VIR04_03175"
     CDS             4813..>4983
                     /gene="rpoZ"
                     /locus_tag="VIR04_03175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY9459784.1"
                     /translation="MARITVEDCLEKIPNRFNLTLAATYRARELAQGHEARLETKDKP
                     TVTALREIAAGVT"
BASE COUNT         1146 a         1500 c         1297 g         1040 t
ORIGIN      
        1 cgcttcagcc tgcgccgccg cggctcgcgc attgtcataa tcctggctgc tgatcgcttt
       61 tgcccggatt aatttggcgt agcgctgggc cagcaagctg gcgccatgcg cgtccgcctg
      121 agctctttta agttgcgctg cggcctgatc gcgcacggct cgatacggag tgggatcgat
      181 cgtgaacagc aactggccgc gcacgacgtc gctaccctgt ttgaaattgg tctgactgac
      241 gataccggtg acgcgggcgc gaatttgcgc ctctcggatt gcttcaacgc gtccgggcag
      301 gtcaacgaaa atttcggtgc gagccggctc gacggtaacg acgctgaccg gaaccttgat
      361 ctcgccagag tgctgttgat ccttgtcgcc gcacgcgcca agcagaagca acgatgaaaa
      421 aatgacaatt tgaattttgt gccgcattaa cggacgcaga ctggacatga aacagctccc
      481 acacatggcg ccccgggcgc cgctgtgacg gttgtgattt ttagtaaacg gggagtataa
      541 ggcgaaatga gcgccattat cctgaaatga gtttctttac ggaacattaa acaacgctac
      601 gaaccagcgc tagctacagt aacgattcag ttacttatcg aataatttct gaagcagact
      661 tgataattgc atccgagaat atgacgcctt gttttgcggc ggcgtcggga tttacataca
      721 acgcaagctt gttgcccgca acaatgccca gggcggccgc cgcaccgcgc ttgacgccgt
      781 cgatgtcatc cgcaatcaag gggattttcg cattctcgcc cactttgaca atagattcat
      841 acgccgatac aacgttgctg tcgtccgtcg tgtagatgac atccactttg ccaatcaaat
      901 tacgcgcggc cgctcccacc tctgttgctt gcggcgccgt tgcttccgac agggacatcc
      961 ccgctttcgg caaaagttcg cgcatctgtt tgactacggc aaccgaagcc ggattcgcag
     1021 ggttgtacac catgccgacg cgtttggcct gcgggacaag ctttctgatg agatcaattt
     1081 gcttttcgag gggcggcgtc gcgcgttcct ccgttactgt gacttcggtt gcccgggcgt
     1141 gcgcgggcat gtgcagcatg ccgcccgcaa cagccacaat tcccagaagc aagcgcgcgg
     1201 atttcatcag acttttctgc tgcgatctcg gaagtcgcgt tgccgcagca gcgatttggc
     1261 ccagcgaacg gccggcaaac catagcggct ttccaccagg cgggcccgat ccataaactg
     1321 cagtgcgcca atctccaaca aggggccttt gaaagccaga ataacgcgat tgccgacatc
     1381 aatttcggga agctcaagca cgcctccgtc gaaaacggcg cggatgttgg cgaggtttcg
     1441 tggaaaactt tcatgatcgc cgaacagatt gacggtcatc acgcccgaat cacttaaaga
     1501 acgatagcaa ccctgataaa actccagcga ctcatgcacc ggccctctgg catgcgcgtc
     1561 gtaaatatcg accatcaatg cacagcagcg gcccgcattc cggctttgcc ggacccagtt
     1621 ggcggcatcg cattgatcga cggtcgagcg cgctgacttc gacagccgga agtaagcgcg
     1681 gcaagcggca atgacgcgcg cattccactc gaccgtgacc accgaactgc gcgtttgttt
     1741 taatgcaaag cgcacaagcg agccggcgcc caagcccaaa atgccaattg tgctttcttt
     1801 acccggcgcc agaaacaaca gccacgccat catctgccgc gcgtaggcca atacgagttc
     1861 agagggattt ttgattcgca tggcgccctg cacccatggt gtcgagaagt gcagataacg
     1921 aatgccgtcc agttcggaaa tcgtcggttc gtcagcgggc gaaggcaagg acgggttcac
     1981 gcgctcatcc attaaagagg ccaccgccca tagtcagatt cgctcgaaaa tggcagcaat
     2041 tccttgccct ccgcctatgc acatggtgac cagcgcgtag cgcccctgga tacgattcaa
     2101 ctcgtgcaac gccttgacca caagaatcgc acccgtggcg ccaatgggat gcccaagact
     2161 gatgccgctg ccattcggat tgactttttc aggatcaaag cccaattcct tgctaacggc
     2221 gcacgcttga gcggcaaacg cttcattggc ctcgatcaca tcaaactgac cgatgtcaag
     2281 gccggatttg cgcaatgcca ttctggtggc cggcacgggt cccatcccca tgtatttcgg
     2341 atcgactccg gcatgcccgt aggccaccaa acgcgccatc ggcttgattc cgcgatcccg
     2401 cgccgccttc tgcgtcatca gcaacaccgc cccagcgccg tcgttcaagc ccgaagcatt
     2461 gcccgccgtc accgtgccgt tttctttcat aaataccggt tttagtgccg aaaaatcagc
     2521 cgccgttgca tcaaaacgca catgttcatc gacgtcaaac accacctcgc ccttgcgggt
     2581 tttctgcacg atcggaacaa tttgctcttt gaaatagccc gccttgatcg cagcggcggc
     2641 gcgctgatgc gaacttagcg caagtgcgtc ctgttcggcg cgcgaaaccc cgtatttgcg
     2701 agccacgttt tcggctgtga tgcccatgtg ctctttatcg aaaggatccg ataaagcgcc
     2761 cgtcatcata tcgagcagaa cactatcgcc catgcgcgca ccccaacgct gggccggggc
     2821 aatatagggc gcgcggctca tgctttcggc acccgcaccg atcgcaatat cggtatcgcc
     2881 cagccacaac gcctgtgctg ccgaaacaat cgcctgcaaa cccgaaccgc acagacggtt
     2941 gacgttaaac gcgggggtag actggccaat acccgcattc atggcggcaa cgcgtgacaa
     3001 atacatatca cgcggttctg tattaatgac atggcccacc accacatgac ccacatcgct
     3061 gccgggaatc ccggtgcgct caagcaccgc gcgaataacc gtcgagccca aatcacaagg
     3121 cgccatattt ttcagcgccc caccaaaatc gcccaccgcg ctgcggcaag cgccaacgac
     3181 aaccacatct tccattgcca tcctccagaa agattcatgc gtgcatgaca tacaacttta
     3241 ataataaccc ggtgcgcacg agctcaaggc gagtcggtta aaatcctgaa aattcacaca
     3301 ttgccgcgcc aactcaaatg aagctagcca cctggaatat taattcgctt aaagtgcgat
     3361 tgccgcaggt tctcgagtgg ctgaaaaatc acccggtaga tgcgctttgt ctgcaagaat
     3421 taaaactgcc cgatgacaga tttccatctg aagcctttgc tgccgcggga tatcaggctt
     3481 gctgggcagg ccagaaaacc tacaacggag tggcgatcat ttcacgcgac cccgcgcgtg
     3541 atgtgcaacg cgacctgccc ggtttagatg atccgcaaag gcgcattatc gccgccactt
     3601 tgcccggccc cgccggcgac atccgggtga tcagcgccta ctgccccaac ggccagacgg
     3661 tcggtagcga taaataccac tataaattga aatggtttgg cgtgctcagg gaatggctac
     3721 gcgaagaact gagccattac ccgcatctgg ccgttttggg cgactacaac gtggcgccag
     3781 cagatgaaga cgtgcacgac cccgctaaat gggtgggcga agtgctggtc tccgagccag
     3841 aacgcatggc attccgctcc ttgctggatc ttggtctgac agacgctttt cgtgaattcg
     3901 aacaagaaaa aaaatcattt agctggtggg attatcgacg gcttgctttc cagcgcaacg
     3961 ctggactgcg tatcgatcat gtcctgttat cagatgcatt gcgcatgcat tgcagcgcgt
     4021 gcgtcatcga caaagagccg cgcagaaatg cacaaccgtc agatcacgca ccggtgatcg
     4081 caacgctgac aacctgaatt tgctattctt gcaaccttgt ctgtaacgaa taaataaaat
     4141 gtcgactaac cctgcccacg ccaatatctt catggtggtc gcccccagcg gggccggtaa
     4201 atcaagcttg gtaaacgcct tgctactaca ggacgcgtcg ctcttccttt ccatttcgtg
     4261 caccacacgc gagccgcggc cgggtgagca ggccggcaaa cactaccggt ttgtcgccaa
     4321 ggaagagttc tttgccatgc gccaaaacga cgcgctgctg gaatgggcgg aagtgcatgg
     4381 ccatttttac ggaacgccgc gcgacaacat cgaccgcgcc atgcagcaag gacgcgatgc
     4441 cttgcttgaa attgattggc agggcgcgcg tcaggtaaag aagtattttc cccatgccat
     4501 aggcatattc atcctgccgc catccattga agcgctcgaa aaacggctct atagccgtgg
     4561 tcaggatgca ccccaagtga tttcccgccg gctcctggcc gcgggcggcg aaatgtctca
     4621 cgcctcagag tgtcaatatg ttattattaa tcaagaattt agcgtcgcgt caaatcagct
     4681 tgcgcagata gttgcggcaa cccggttgcg ctacgaaacg caggcggtgc gcaacgccgc
     4741 gctcttttcc caactgggca ttagcacgac cactgcctag gatccttgtc cctttgtttt
     4801 aataggttat tcatggctcg tattaccgtc gaagactgct tagagaaaat ccccaaccgc
     4861 ttcaacctga cgctggcggc aacgtaccgc gcccgcgaac ttgcgcaagg ccatgaagcg
     4921 cgactcgaga ccaaagacaa acccacggtc acggcgttgc gcgaaatcgc cgccggggtg
     4981 acc
//
LOCUS       DATCXU010000144         6936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_767889,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000144 DATCXU010000000
VERSION     DATCXU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767889"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VIR04_03180"
     CDS             <1..314
                     /locus_tag="VIR04_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEY9459785.1"
                     /translation="LITEETVQSMKPGSVIVDLAVERGGNCPLSEKDQIVVKHGVTLI
                     GLSNLPALVATDASDLYARNVLDFMKLITNAEGELAIKHDDEIIAACLMCMDGKLLRS
                     A"
     gene            314..631
                     /locus_tag="VIR04_03185"
     CDS             314..631
                     /locus_tag="VIR04_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY9459786.1"
                     /translation="MEAVDPTLINVIIFVLAVYVGFHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLATALTEGNLARFMGVFAVALAAVNVFGGFLVTRRMLEMFKKKEKKSAPG
                     GRP"
     gene            628..2067
                     /locus_tag="VIR04_03190"
     CDS             628..2067
                     /locus_tag="VIR04_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY9459787.1"
                     /translation="MISANLVTFLYLVASVCFIQALKGLSHPTTSRRGNTFGMIGMAI
                     AILTTASLIIGLARDGAAGMGLGWIVLGLLIGGTVGTLLARRVEMTKMPELIAFMHSM
                     IGLAAVAIAVAVVAEPHAFNIAAVGDPIPVGNRLELFIGTFIGAITFSGSVIAFGKLS
                     GTYKFRLFQGAPVVFAGQHMVNLLLAIAMLACGFWFMATQMWLPFIIMALMAFALGVL
                     IIIPIGGADMPVVISMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSFIMCKA
                     MNRSFINVIMGGFGATPGGNSNGDQHAQRNVKSGSPEDAAFLMNNAETVVIVPGYGLA
                     VARAQHALKELTQKLAENGVTVKYAIHPVAGRMPGHMNVLLAEAEVSYDQVFEMDDIN
                     SEFAQTDVVLVVGANDVVNPAAKNDPNSPIAGMPILEAYKARTVIVSKRSMASGYSGL
                     DNELFYMDKTMMVFGDAKKTIEDMVKALE"
     gene            complement(2069..2986)
                     /locus_tag="VIR04_03195"
     CDS             complement(2069..2986)
                     /locus_tag="VIR04_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY9459788.1"
                     /translation="MRVLVMGGTGFIGRHVVARLVAAGHFVHVPTRHYQNGRDLLVFP
                     TVNLFVADLYDDAVLTRLIGECDAVINLIGVLYGRSGRPYGEDFAKVHVELPGRVART
                     CRSLGISRLIHISALGADVNGPSAYSRSKAAGEAAIIDAFSGYADGTYTIFRPSVVFG
                     AEDHFMNLFATLARWLPVIPMAGARARLQPIYVGDVAAVIANALVNLKTAGQIYPLAG
                     LQAYRLGELVRLASVWSGHPRHVVNLPMAVGRLQALFFECLPGQPLISRDNLDSLTVD
                     NVSDDPIAPELGFVPQSLESIAPVYLARD"
     gene            complement(3124..4215)
                     /gene="ychF"
                     /locus_tag="VIR04_03200"
     CDS             complement(3124..4215)
                     /gene="ychF"
                     /locus_tag="VIR04_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY9459789.1"
                     /translation="MALQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVV
                     EVPDKRLSALAEIVTPERIVPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVH
                     VVRCFDDENVIHVAGKIDPLSDIEVINTELALADMASAEKALHRFQKTARAGDKEAAK
                     IAALLEKVVAVLDQARPIRSLRLDDDERALLKPLCFITAKPTMYVANVKEGGFENNPH
                     LKAVQDYARKENAPALALCAAIEADIADLDDTDKQVFLDDIGMDEPGLDRVIRGAYAL
                     LGLQTYFTAGVKEVRAWTIRVGDTAPKAAGVIHTDFERGFIRAQTIAYEDFIACKGEH
                     GAKEAGKMRAEGKDYVVKDGDVLNFLFNV"
     gene            complement(4288..5058)
                     /locus_tag="VIR04_03205"
     CDS             complement(4288..5058)
                     /locus_tag="VIR04_03205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY9459790.1"
                     /translation="MAEGPLERDGRFRAELHDFFRFVARPRLAPRLPGRRAANGRWED
                     WFPAVSGRRMLQWALFLWAVNLIVLGPIAVIAATEGGAQHRLDLANVPWLQALLWAPV
                     VEELVFRYGLRHIVQALWVAPLAVYALLAGPQWVSFFLVLAILLICWRPYFGDGRAPR
                     PLPWRIRVRYRHWFPLVFHLSSLTFAAMHLNNFSMHGTSYWLMPLLVLPQWLTGMVLG
                     WLRVKRGIGASMLLHGIFNGGPLLIVWIVLHTVPALAT"
     gene            complement(5051..5647)
                     /gene="pth"
                     /locus_tag="VIR04_03210"
     CDS             complement(5051..5647)
                     /gene="pth"
                     /locus_tag="VIR04_03210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY9459791.1"
                     /translation="MMTSIRLIVGLGNPGPEYETTRHNAGFWLANHLADDLKASFSLE
                     KAFSAWVAKARFEGETVLLAKPATFMNHSGQAAGALMRFYKLTPEQVLVLHDELDLLP
                     GNVKLKQGGGHAGHNGLRDIQAAFSSPDFWRLRIGIGHPRTLGLKQSVVDFVLGSPRR
                     EELKAIEQVIDRCRAIVPAMLRGDYPLAINQLHEANCG"
     gene            complement(5747..6394)
                     /locus_tag="VIR04_03215"
     CDS             complement(5747..6394)
                     /locus_tag="VIR04_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY9459792.1"
                     /translation="MKINATERSVQGTSASRRLRHAGRVPAIVYGAKAPAVTIELDHN
                     EIYHALRKEEFHASILTMELGGKSEQVLLRAVQWHPYKQQVLHVDFLRVDATQAINTK
                     VPLRFINGDASPAVKLSGAIISHVMTELDITCLPANLPQYIDADLGQLLAGVSFHLSD
                     ITLPKGVAYIARGGDTDPVLAVGLVKSGTDAEETDASAAPAAGDAAASAPEKPAE"
     gene            complement(6504..>6936)
                     /gene="prs"
                     /locus_tag="VIR04_03220"
     CDS             complement(6504..>6936)
                     /gene="prs"
                     /locus_tag="VIR04_03220"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY9459793.1"
                     /translation="GGVVRARALAKQLEADLAIIDKRRPRANVSEVMNIIGEVDGRTC
                     IIMDDMVDTAGTLCKAAQALKQRGAKKVYAYCTHPVLSGSAVPRIMESDLDEVVVTDT
                     IPLSEAARTCGKIRQLSCASLLGETILRISNAESVSSLFVD"
BASE COUNT         1619 a         2021 c         1912 g         1384 t
ORIGIN      
        1 ttctgatcac cgaagaaacc gttcaaagca tgaaacccgg ctcggtcatt gtcgacctgg
       61 cagtggaacg cggcggcaac tgccctcttt cagaaaagga ccaaatcgtc gtcaagcatg
      121 gcgtcacact gataggactg agcaacctgc ccgcgctggt ggccaccgac gcctcggacc
      181 tatatgcgcg caatgtgctt gacttcatga aactgatcac caatgccgaa ggagagcttg
      241 ccatcaaaca cgacgacgaa atcatcgccg catgcctgat gtgcatggac ggcaaattgc
      301 taaggagcgc ctgatggaag cggtcgaccc taccctgatt aatgtcatca tttttgtgct
      361 ggcggtttat gtgggatttc acgtggtctg gaacgtaaca cccgcattgc atacgcccct
      421 gatggcggtg accaacgcca tctctgccat cgtcatcgtg ggagccatgc tggccaccgc
      481 gctgactgag ggcaacctgg cgcggttcat gggcgtattc gcggtagcgc tggcggccgt
      541 aaacgttttc ggcggattcc tggtgacgcg ccgcatgctg gaaatgttca agaagaaaga
      601 gaaaaaatca gcgcccggag gacggccatg atttcagcaa acctggttac ctttttatat
      661 ctcgttgcct cggtctgttt tatccaggcg cttaaaggct tgtcccatcc caccacttcg
      721 cggcgtggca atacttttgg catgatcggc atggccatcg ccattctaac cacggcctcg
      781 ctgattatcg gtcttgcgcg cgatggcgcc gccggaatgg ggctaggctg gatcgtgctg
      841 ggtttgctta tcgggggcac tgtcggcacg ctactggcca ggcgcgtcga aatgacaaaa
      901 atgcctgagc tcatcgcttt catgcacagc atgatcggcc ttgccgccgt ggccatcgcc
      961 gttgccgtcg tagcggaacc tcacgcattc aacattgccg cggtgggcga tcccataccc
     1021 gtcggcaacc ggcttgagtt gtttattgga accttcatcg gcgcgattac tttttcaggc
     1081 tcggtcattg ccttcggcaa actgtcgggc acatacaaat tccgtttatt ccagggcgct
     1141 ccggtcgtgt tcgccggcca gcacatggtc aacctgctgc tggcgatcgc catgctggcc
     1201 tgcggattct ggttcatggc cacacaaatg tggttgccat ttatcattat ggcgctaatg
     1261 gccttcgcgc tgggcgtgct cattatcatt ccgatcggcg gggccgatat gccagtcgtc
     1321 atctccatgc tgaacagtta ttcggggtgg gcagcggcgg gaatcggctt ctcgctgaac
     1381 aaccccatgc tgattattgc ggggtcgctg gtcgggtcat cgggcgccat tctgtccttc
     1441 attatgtgca aggcaatgaa ccggtcattc atcaatgtaa tcatgggcgg attcggagca
     1501 acgccgggcg gcaattcaaa tggcgatcaa cacgcgcaac gcaacgtcaa atcgggcagc
     1561 cctgaagacg ccgcgttcct gatgaacaac gccgaaacgg tcgtgatcgt tccgggctac
     1621 ggccttgccg ttgcgcgtgc tcaacatgcg ttgaaggagt tgacccaaaa gctcgccgaa
     1681 aacggggtca cggtcaaata cgcgatccat ccggtggcgg gccgcatgcc cggccacatg
     1741 aatgtgttgc tggccgaagc cgaagtttcc tacgatcaag tatttgaaat ggacgacatc
     1801 aacagcgaat tcgcgcagac tgatgtcgtt ttggtggtag gcgcgaacga cgtggttaat
     1861 cccgccgcca aaaacgaccc caactcgccc atcgcaggca tgcctattct ggaagcctac
     1921 aaggcccgca ccgtgatcgt gagcaaacgc tcgatggcat ctggctactc tggcctggac
     1981 aatgaattgt tctacatgga caaaaccatg atggtctttg gcgatgctaa aaagaccata
     2041 gaagatatgg tcaaggcgct ggagtaggtt aatcgcgggc cagataaacc ggcgcaatgg
     2101 actccagcga ttgcggcacg aaaccaagtt cgggggcaat ggggtcgtcg ctcacattat
     2161 cgacggtaag cgaatcgaga ttgtcgcgcg atattaaggg ctgtcccggc aggcactcga
     2221 aaaacaaagc ctgcaagcgg cccaccgcca tgggcaggtt tacaacatgc cgggggtggc
     2281 cgctccatac cgaagccagt cgaaccaatt cacccaagcg gtaagcttgc agtccggcaa
     2341 gcgggtaaat ttgcccagcc gtctttagat tgaccagagc atttgcgatg accgcggcga
     2401 catcgcccac ataaattggc tgcagacgtg cgcgcgcgcc ggccatgggt atgaccggca
     2461 gccaacgggc cagagtggcg aacaaattca tgaaatgatc ctcagctcca aacacgactg
     2521 acggacgaaa tattgtgtac gttccatcgg cataacccga aaatgcgtcg atgatggctg
     2581 cttcgcctgc ggctttggag cgtgaataag cgctgggccc attgacgtca gccccaaggg
     2641 cgcttatgtg gataagccgc gagatcccca gcgaacggca agtgcgcgcc acacgcccgg
     2701 ggagttcaac atgcaccttg gcaaagtcct cgccataggg acgcccgctg cgaccataca
     2761 gaacgccgat caaattaata accgcatcgc attcgccaat aagccgtgtg agcaccgcat
     2821 cgtcatacaa gtcggccacg aataaattga ccgtcggaaa caccagcaag tcccggccat
     2881 tttgataatg tcgcgtaggc acatgaacga aatgccctgc agcgaccaaa cgcgcaacaa
     2941 cgtgccgtcc aataaacccc gttcctccca tcaccaaaac acgcattggg ttctccgata
     3001 aagttgacca cgcgatgcaa gcaatacatc gcgcgtcatt cacatgccta gcagtatagg
     3061 ctttacacgg gccagtgcag cattcgcttc aggtcataaa cgcgccgcaa tccaatgcct
     3121 gccctagacg ttgaacaaaa agttcaatac gtccccatct ttaaccacgt aatccttgcc
     3181 ttcggcgcgc atttttccgg cttcctttgc cccgtgttca cctttgcacg caatgaaatc
     3241 ctcgtaggcg atggtttgag cgcgtatgaa tccgcgctca aaatcggtgt gaatcacgcc
     3301 ggccgccttg ggggcggtgt cgcccacgcg aatagtccag gcgcgcactt ccttgacgcc
     3361 ggccgtgaaa taggtctgca agcccaataa cgcataagcg ccgcggatga cgcggtcaag
     3421 gcccggctcg tccattccga tatcgtccag gaatacctgc ttgtccgtgt cgtccagatc
     3481 ggcgatatcg gcttctatgg ccgcgcacaa cgccagggcc ggcgcatttt ccttgcgcgc
     3541 gtagtcttgc accgccttta gatgagggtt gttctcaaac ccgccttcct tgacattggc
     3601 cacgtacatc gtcggcttgg cggtgatgaa gcacaaaggc tttagcaatg cgcgctcgtc
     3661 gtcgtccagg cgcagggaac gaatggggcg cgcctgatcc agaaccgcca ccactttttc
     3721 aagcagcgcc gcaatcttgg ccgcctcttt atcgcccgca cgcgccgttt tctgaaaacg
     3781 atgcaaggcc ttttcagcgg aagccatatc cgccagcgcc agctcggtgt tgatcacttc
     3841 aatatcggac aacgggtcaa ttttaccggc gacgtgaatc acattctcat catcgaaaca
     3901 gcgcacaaca tgaacgatgg cgtcggtttc acgaatgttg gccaagaatt gattgcccaa
     3961 cccttcgccc ttggacgcgc cggcaaccag accggcgata tcgacgaatt cgaccacggc
     4021 gggcacaatg cgttcaggcg ttacgatttc ggccagcgcc gacaaacgct tgtcgggaac
     4081 ttcaaccacg ccgacattgg gttcgatcgt gcaaaaggga tagttctccg ccgcaatgcc
     4141 cgctttggtc aacgcgttaa aaagagtgga ttttccaacg ttgggcaagc caacaatgcc
     4201 gcattgcaat gccatgagta tcctgagcta ataactgaaa ctgcgattga acaaaagact
     4261 attttaacca gtgcggcgcc agccgcttca tgtggccagc gccggaacgg tatgcagaac
     4321 gatccacacg atcagcagcg ggccgccatt aaaaataccg tgcagcagca tggaagcgcc
     4381 aatgccccgc ttgactctca gccatcccaa aaccatgccg gtaagccact gcggcaatac
     4441 cagcagcggc atgagccaat acgaagtgcc atgcatggaa aaattattca agtgcatggc
     4501 ggcaaacgtc agggatgaca gatgaaatac cagggggaac cagtggcggt atcgtacgcg
     4561 aattctccat ggcaacggac ggggcgcccg cccgtcgccg aaatagggcc gccagcaaat
     4621 cagcaaaatt gccaaaacca gaaaaaaact gacccattgc gggcccgcga gcaacgcgta
     4681 cactgccaac ggcgccaccc acaaagcctg cacaatgtgg cgcaaaccgt agcgaaacac
     4741 cagttcttca acgactggcg cccacaacaa cgcctgcaac cacgggacat tcgccaggtc
     4801 cagtctatgc tgggcgccgc cctcggttgc cgcgattacg gcaataggtc ccaaaacaat
     4861 taaattgacg gcccacagaa aaagcgccca ctgcagcatg cggcggcctg atacggcggg
     4921 aaaccagtct tcccagcggc cattggcggc gcgtcgtccc ggcaagcgtg gagcaaggcg
     4981 aggccgggcg acaaagcgaa aaaaatcatg aagctcagca cgaaaacgcc cgtcgcgttc
     5041 gagcggccct tcagccacaa ttcgcctcat gcaactgatt gatcgcaagc ggataatcgc
     5101 cgcgcagcat tgccggcaca atggcgcggc aacgatctat aacctgttca attgccttga
     5161 gctcctcgcg gcgcggcgag cccagcacaa aatcaaccac agactgcttc aaacccaggg
     5221 ttcgagggtg gccgatgcca attcgcagac gccagaagtc ggggctggaa aacgccgcct
     5281 gaatatcgcg caaaccatta tggcccgcat gcccgccgcc ctgctttaac ttgacgttac
     5341 ccggcaacag atcgagttca tcgtgcagaa ccagtacttg ctcgggcgtc agtttgtaga
     5401 agcgcatgag cgcgcccgct gcctggccgg aatggttcat gaaggtggcc ggcttggcca
     5461 gcaagacagt ttcgccctcg aatcgggcct tggcgaccca ggctgaaaac gctttttcaa
     5521 gggaaaagct ggccttcagg tcgtcggcca ggtggttggc caaccagaag ccggcgttat
     5581 ggcgtgtcgt ttcatattcg ggaccgggat tgcccaggcc gacgatcaag cgtatggaag
     5641 tcatcataga taagcagatt aaaacaccaa taagcaaaac ccctgccggc atgcgccaga
     5701 caggggttta tgccaagccg gcaggctggc gaacaagggg agacctttat tcggctggct
     5761 tttcgggcgc tgaagcggcg gcgtcgcctg cggccggagc ggccgacgcg tcggtttctt
     5821 ccgcgtcagt acctgattta accagcccga cggccagcac gggatcggta tcgccgccgc
     5881 gagcgatata agccacgccc ttgggcaagg taatgtcgga caaatggaag ctgacgccag
     5941 ccagcaattg acccaggtcg gcatcgatgt attgcggcaa gtttgccggc agacatgtga
     6001 tgtccagttc cgtcatcacg tgactaatga tggcgccgct aagcttgaca gcgggcgatg
     6061 catcgccgtt gataaagcgc aaaggcacct tggtgttgat tgcctgggtt gcgtcgacac
     6121 gcaaaaagtc gacgtgcaat acttgctgtt tgtaaggatg ccattgcacc gcccgcaaca
     6181 acacctgttc ggactttccg cccagctcca tggtcaggat cgatgcgtga aactcttctt
     6241 tgcgcaaggc gtggtagatc tcgttgtgat ccagttcgat agtgacggca ggcgctttcg
     6301 caccatacac aatagcggga acacggcccg cgtggcgcag gcggcggctc gcactcgtgc
     6361 cctgtacgct acgttctgtg gcattgattt tcatgtaaaa gaactccaaa aactaaaggc
     6421 ataaagcccg attaaaaatg cggcgccgtt ttaacgccac gaaagttcat tgctcgccgc
     6481 gaccagccag caacgaattg aattcagtcg acaaacaaag agctgaccga ttcggcgttt
     6541 gaaatacgca aaatggtctc gcccaacagt gaagcacaag aaagctgacg aatcttgccg
     6601 caggtgcgtg ccgcttccga cagtggaata gtgtcggtca ccaccacttc gtcgaggtcg
     6661 gactccataa tgcgcggtac ggcggagcct gacaaaacgg ggtgcgtgca ataggcatac
     6721 accttcttgg cgccgcgctg cttgagagcc tgggcggcct tgcacaaggt acccgcggta
     6781 tcaaccatat cgtccatgat gatgcaggtg cggccatcga cctcgccgat aatgttcatg
     6841 acttcagaca cattggcgcg cggacggcgc ttgtcgataa tagccagatc ggcttcgagc
     6901 tgcttggcca gcgcccgggc gcggacgact ccgccg
//
LOCUS       DATCXU010000145         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_109288,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000145 DATCXU010000000
VERSION     DATCXU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109288"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VIR04_03225"
     CDS             <1..1272
                     /locus_tag="VIR04_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY9459794.1"
                     /translation="LLASLREPEPPRGFRDALGKVAMLKAALWDMSPKNIKNAACQEI
                     VLEGQDVDLDRIPLQRCWPNDVAPLLTWGLVVTRGPNAKRQNLGIYRQQQIGRNKLIM
                     RWLSHRGGALDFRSHCLAHPGAPFPVAVALGADPATILGAVTPVPDSLSEYQFAGLLR
                     GSRTEVTKAISSDLSVPAFAEIVLEGHLLPASDPKAIAPIVPQGNAGPRETQYEMALE
                     GPFGDHTGYYNEQDWFPVFTVERITMRRNAIYHSTYTGKPPDEPSVLGMALNEVFVPL
                     LKRQFPEIVDFYLPPEGCSYRLAVVSIHKQYLGHAKRIMFGLWSVLRQFMYTKFIVVV
                     DADINPRNWPEVIWAMTTRMDPARDTVLVENTPIDYLDFASPVSGLGGKMGLDATNKW
                     PGETQREWGTPIEMSASVKARVDAIWQELGL"
     gene            complement(1269..1619)
                     /locus_tag="VIR04_03230"
     CDS             complement(1269..1619)
                     /locus_tag="VIR04_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459795.1"
                     /translation="MNKNISIIERPERIELARARAALERQSLERGIRNLEESLKPATL
                     FRGLLPKSVAQSGSLGWLGQGLLLTQRYPLLASGASALISGLGKRHRWLRLGAGLLLG
                     WQLARSGVLKKKPG"
     gene            complement(1616..>2076)
                     /locus_tag="VIR04_03235"
     CDS             complement(1616..>2076)
                     /locus_tag="VIR04_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY9459796.1"
                     /translation="GHGRQTVSKEIVMALARSIGGLAGTLVSIARTRIELFSLEASEQ
                     KGRLIKLICMVLGGVLFLMLAIVALSLLIVAYFWSTEYRYLASWLLVLFYGALGLGLL
                     LAVRHALVSEPGPFAATLDELRRDMAMIDRLREPVSGASGTDRPVPAERL"
BASE COUNT          478 a          649 c          568 g          381 t
ORIGIN      
        1 ttgctggctt cgctgcgcga gccagagccg ccgcgggggt ttcgcgacgc gttgggcaaa
       61 gtcgcaatgc tgaaagccgc attatgggat atgagtccca aaaacattaa aaacgccgcc
      121 tgccaggaaa tcgttctgga aggccaggat gtcgacctgg accgaattcc gctccagcgc
      181 tgctggccca atgatgttgc tccgctacta acctggggcc tggtggtaac ccgcggtccc
      241 aatgcaaaac gccagaatct gggcatctat cgacaacagc aaatcggccg caacaaactg
      301 attatgcgct ggttgtcgca ccgtggcggt gcgctcgatt tccgcagcca ctgcctggcc
      361 cacccgggcg cgccattccc ggtcgcggta gctttgggcg ccgatcccgc caccatactg
      421 ggcgccgtca cccccgtacc cgacagcctg tcggaatatc aattcgcggg cttattgcgc
      481 ggctcgcgca cggaagtgac caaagcaata agcagtgatc tgtccgttcc ggcttttgcc
      541 gaaatcgtgc tcgaaggcca tttgctgccc gccagcgacc cgaaggcgat cgcgcccatt
      601 gtgccccaag gtaatgccgg accgcgcgag acccagtatg aaatggcgct cgaaggaccc
      661 ttcggcgatc acaccggcta ctacaacgaa caggattggt tccccgtatt tacggttgaa
      721 cgcatcacca tgcggcgcaa cgcaatctac cacagcacct ataccggcaa gccacccgac
      781 gagccgtcgg tattaggtat ggcgctcaac gaagtgtttg tgccgctgct taaacgccaa
      841 ttccctgaaa tcgttgattt ttatctgcct ccggaaggat gcagctaccg cctggccgtg
      901 gtttccatcc ataaacaata ccttgggcac gccaagcgca ttatgttcgg actatggagc
      961 gtgctgcgcc agttcatgta caccaaattc attgtcgtcg tggatgccga catcaatccc
     1021 cgcaactggc cggaagtgat atgggcaatg accacccgca tggatccggc gcgcgatacg
     1081 gttctggttg aaaacacgcc catcgactat cttgatttcg cctcgcctgt atcgggtctc
     1141 ggcggcaaga tggggctgga cgccaccaac aaatggccag gtgaaaccca acgcgaatgg
     1201 ggcactccca tcgaaatgag cgcatcggtc aaggcgcggg tcgacgccat atggcaggaa
     1261 ctggggcttt agcctggctt tttcttcagg acacccgaac gcgcaagctg ccagccgagc
     1321 agtaatccgg cgcccagccg caaccagcgg tggcgctttc ccagaccgga tatcagcgcc
     1381 gacgcccccg aagccagcaa aggatagcgc tgcgtcagga gcaagccttg ccccagccag
     1441 ccaagggaac cggattgagc aacagactta ggcaagagcc cacggaaaag agtggcgggc
     1501 tttaacgatt cttcgagatt cctgatgccg cgctccagac tttgccgttc cagagccgcg
     1561 cgtgcgcgcg caagttcaat gcgctccggc cgctcgatga tggaaatatt tttattcata
     1621 gccgctccgc aggaaccggc ctgtctgtcc cggaggcccc tgaaacgggc tctcgcagac
     1681 ggtcaatcat ggccatgtcg cggcgcaatt cgtcaagggt ggcggcaaac ggccccggtt
     1741 cggaaaccag cgcatgacga acggccagca ataggcccaa accgagcgcc ccgtaaaaaa
     1801 gaaccaatag ccacgaagca agataacggt attcggtcga ccaaaaatac gccacgatca
     1861 acagagaaag cgccactatt gccagcatga ggaacaacac ccccccaagc accatgcaga
     1921 tcagtttgat taaacggccc ttctgctcag atgcttcgag ggaaaacagc tcgatacgtg
     1981 tacgcgcgat ggacaccagc gtacccgcca accccccaat cgaccgtgca agcgccataa
     2041 ctatctcttt tgagaccgtt tgcctgccgt ggccgg
//
LOCUS       DATCXU010000146         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_334583,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000146 DATCXU010000000
VERSION     DATCXU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334583"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..251)
                     /locus_tag="VIR04_03240"
                     /pseudo
     CDS             complement(1..251)
                     /locus_tag="VIR04_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(368..646)
                     /gene="merP"
                     /locus_tag="VIR04_03245"
     CDS             complement(368..646)
                     /gene="merP"
                     /locus_tag="VIR04_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HEY9459797.1"
                     /translation="MTKKLFISLVLVAAAAPAWAATQTVTLSIPGMTCETCPITIKYA
                     LSKVDGVSGADVSFQKREAVVTFDDAKTNVQKLTKATENAGFPSSVKQ"
     gene            complement(656..1006)
                     /gene="merT"
                     /locus_tag="VIR04_03250"
     CDS             complement(656..1006)
                     /gene="merT"
                     /locus_tag="VIR04_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HEY9459798.1"
                     /translation="MSEPKNGRGALFTGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPIFIGAALVAMFFAWRRIFRPVQACKPGQACALRRYRSPYKLVFWIVVMLI
                     LIAIGFPYVVPFFY"
     gene            1078..1515
                     /gene="merR"
                     /locus_tag="VIR04_03255"
     CDS             1078..1515
                     /gene="merR"
                     /locus_tag="VIR04_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HEY9459799.1"
                     /translation="MQTNSANLTIGAFAKAAGVNVETIRFYQRKGLLLEPDKPYGGIR
                     RYGGADVARVRFVKSAQRLGFSLDEIAELLRLEDGAHCDEASSLAEHKLQDVREKMVD
                     LVHMEAVLSELVRACHARTGSVSCPLIASLQGAKETFNAGAGI"
     gene            1534..2583
                     /locus_tag="VIR04_03260"
     CDS             1534..2583
                     /locus_tag="VIR04_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY9459800.1"
                     /translation="MRFQEGLRQAGVPAALGAALLFGAGTPLAKLLLNSVSPWLLAGL
                     LYLGSGVGLGLYRYFIKAPAVKLARREITWFAGAVIAGGIVGPVLLMFGLTGMPASGA
                     SLLLNAEGVFTALLAWFAFKENFDRRIALGMAAIVAGAVILSWPGEARFAGAWPALAI
                     LGACFAWGLDNNLTRKVSLSDATWIASVKGLVAGSVNLVLALVVGASVPSLPNLAGAL
                     VVGFFAYGVSLALFVVGLRHLGTARTGAYFSIAPFMGALLALAMGEPLTLPLLIAGVL
                     MALGIWLHLTERHEHLHKHEILEHDHEHTHDAHHQHEHDVYVAPGTTHRHWHQHTPVT
                     HFHAHFPDAHHQHRH"
     gene            complement(2766..3407)
                     /locus_tag="VIR04_03265"
     CDS             complement(2766..3407)
                     /locus_tag="VIR04_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY9459801.1"
                     /translation="MTTNRTIKNALYEQVARIAKAAASPKRLEIIELLCQSSKTVAVL
                     AREADISVKLVSAHLKALRSARLVEAERQGKYIVYRLASDDVARFWVTIRLLAEDRLF
                     ELQDALRQLSATTHEWIGNNRNELLTKARNGEVIVIDVRPYDEYEADHLPFARSMPLA
                     ELKIRLAELPKDKPVIAYCRGPFCLMSADAVQLLRSNGIEAVQLREGIAEWKA"
     gene            3590..4333
                     /locus_tag="VIR04_03270"
     CDS             3590..4333
                     /locus_tag="VIR04_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9459802.1"
                     /translation="MFFKQRVNEDASISYLFGCGGYGEAIAVDVLAGDEAWYIKEARK
                     LNVAITTVFDTHIHADHLSGGRKLADFVGAAYALHESNKGKTGYAFTPVADHEMIVAG
                     NTRAQVLHTPGHTEDSICLLVSDFRRGEAPWFVLTGDTLFVGSVGRPDLAGREKEMAA
                     HLWQSLRERLLTLPADLEIFPGHQAGSACGADISGKPSSTIGFEKRWNPLLSLDKEEF
                     ISAVTASIPPRPGDMEHLVAANLGLQALV"
     gene            4350..>4836
                     /locus_tag="VIR04_03275"
     CDS             4350..>4836
                     /locus_tag="VIR04_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459803.1"
                     /translation="MSYRSVAKIGVELGLRANWRQFSLLVLVNAFVGAMVGMERSILP
                     LIAEQEFKLAAHTAILAFIVVFGVSKALTNYFAGRFSDVYGRKIILVAGWLIATPVPF
                     LLMWAPSWNWVLLANVFLGISQGLTWSTTVIMKIDLAGPKNRGMAMGINEFSGYFAIG
                     AA"
BASE COUNT          968 a         1239 c         1444 g         1185 t
ORIGIN      
        1 gcctgccgct gggtcagcag tttgccgcgg ttaattgccg gcgtcgtcgc cgtgatgccg
       61 ccgtcgaatg ggctttcgcg gcgcatgtgg gcgatgtggg cggcgcgaat catgatcttg
      121 gacggtacac agccggtatt gacgcacgtg ccgccgatgg ttcctcgctc gatgatcgtc
      181 acgtgggcgc cgagctcggc cgcctttagc gccgccgcca tcgctgcgcc gcccgtgccg
      241 atgacggcga tatgctgggc gcaatccgcg caacccatcg ccgaaacgat gacaccgatt
      301 acgccgactt tatcggcact gcgtgtggca agtcccacaa tattgctcct tgagttggtt
      361 cagaggctca ttgcttgaca ctggacggga agcccgcgtt ttcagtcgcc ttggtcagtt
      421 tctgaacgtt ggtcttggcg tcgtcgaagg tgacgacagc ttcacgtttc tggaagctta
      481 cgtcggcgcc gctcacgccg tcgactttgg acaaggcata cttgatggtg atcggacagg
      541 tttcgcacgt cattccaggg atagaaagtg tgacagtctg ggtagcggcc catgcggggg
      601 ctgcggcggc gacaagcaca agggaaatga acagtttttt cgtgatgaac cccgatcagt
      661 agaagaatgg cacgacatac ggaaagccga ttgcaatcag gatcaacatg accacgatcc
      721 agaagacgag cttgtagggg gatcgatacc gccgaagtgc gcaggcctgg cccggcttgc
      781 acgcctgtac tggccgaaat atgcgtcgcc aagcgaaaaa catcgccacc agcgccgcac
      841 cgatgaagat ggggcggtag ggttccaaca ccgttaaatt tccaatccag gccccgctga
      901 atcccagtgc gaccagaacc agcggcccaa ggcagcaggt tgaggcgagg atggccgcca
      961 gccctcccgt gaaaagggct ccgcgcccat ttttgggttc agacatgcgc ttgtcctatc
     1021 gaaatcagaa tgaatagcgt aagcttatct ccgtagctat gtacggagtc aagcggtatg
     1081 caaaccaatt cggcgaattt gaccattggc gcatttgcca aggcggccgg agtcaatgtg
     1141 gagaccatcc ggttctatca acgcaagggc ttattgctgg agccggacaa gccttatggt
     1201 ggcattcgtc ggtatggcgg ggcggacgtg gcgcgggtgc gctttgtgaa atcagcccag
     1261 aggcttggct tcagcttgga cgaaatcgcg gagctactca ggttagagga tggcgcccac
     1321 tgtgacgaag ccagcagcct ggccgagcac aagcttcagg atgtgcgcga gaaaatggtc
     1381 gacttggtgc acatggaagc tgtgttatct gagctcgtgc gtgcctgcca tgcgcgtacg
     1441 ggaagtgttt cctgtccgtt gattgcatcc ttacagggag cgaaagaaac tttcaatgcc
     1501 ggagcgggca tttgaatgaa agggattgga gtaatgagat tccaagaagg attacgtcaa
     1561 gcaggcgtac cggcagctct tggcgctgcc ctgttgttcg gggccgggac gccgcttgca
     1621 aagctcctgc tcaattcggt tagtccatgg ttgttggcgg ggttgttata tttgggctcg
     1681 ggtgttgggc taggtttgta caggtatttc atcaaagcgc ccgctgtcaa attagcgcgt
     1741 cgtgaaataa cgtggtttgc gggggcggtc atcgcgggcg gaatcgtcgg cccagtatta
     1801 ttgatgtttg gcttgacggg gatgcccgct tcgggagcgt cccttttgct taatgccgaa
     1861 ggtgtgttca cggcactgtt ggcgtggttc gcattcaaag agaatttcga tcggcgtatc
     1921 gctctgggta tggcggcgat cgtggcgggc gccgtgatct tgagctggcc gggtgaagcc
     1981 cgctttgcgg gtgcatggcc ggcgctggca attcttgggg cgtgttttgc ttggggtctg
     2041 gacaacaacc tgactcgcaa agtatcgctc tccgatgcga catggattgc ttcggtgaaa
     2101 gggttagtcg ccggttcggt aaatctggtc ttagccttgg tggtgggggc aagcgttccg
     2161 tcgcttccga atcttgccgg cgcgcttgtg gtgggatttt ttgcttatgg cgtgagtctg
     2221 gctttgttcg ttgtaggctt gcgccacctt ggaacggctc gcaccggcgc ttacttttcg
     2281 attgctccat tcatgggcgc actcttggcg ttagcgatgg gagagccgct cacattgccc
     2341 ctgcttattg caggcgtgtt gatggcgttg ggtatttggc tgcatttgac ggaacggcac
     2401 gagcatcttc ataagcatga aattttagaa catgatcatg agcatactca cgatgcccat
     2461 caccagcacg agcatgatgt ttatgtcgct cctggtacga cacatcgcca ttggcaccag
     2521 cacacacccg tgacccattt ccatgcacat tttcccgatg cccaccacca gcatcgacat
     2581 tgattgccga acggggctac ggcaaacctt tgtaatgacc tttgtaatga cctgaaacgg
     2641 ggggaaaggc cggttcggta tggcatgtga atttcgacgt tcgtgtagtt taaattgaat
     2701 attcgggcaa tccgttgcgg aagagaccta gctcggatcg cgcagtggcc tgtcacggag
     2761 acgtttcacg ctttccactc cgcgatgcct tcgcgcaatt gcactgcttc aattccgttg
     2821 ctgcgcaaga gttgcaccgc gtcggccgac atcagacaaa acggcccgcg gcaataggct
     2881 attaccggct tgtctttagg gagttccgca agacgtattt tcagttctgc caagggcatc
     2941 gaacgcgcaa atggtaaatg atcggcttca tattcatcgt agggccggac gtcgatgaca
     3001 attacttctc catttcttgc cttggtcagc aattcgtttc ggttgttacc gatccattcg
     3061 tgcgttgtgg cgctgagctg acgcaatgcg tcttgtagtt cgaacaagcg atcttccgca
     3121 agtaggcgaa tcgtcaccca aaaacgcgcg acgtcatcgc ttgccaagcg atagacaatg
     3181 tatttgcctt gtcgctcagc ttccaccagg cgcgcggatc tcaaggcctt caggtgtgca
     3241 ctgacgagct tcacactgat gtcggcttcc cgcgccagca ctgcaacggt tttactcgac
     3301 tgacacaaga gctcgatgat ctcgagccgt ttcggactgg cggcagcctt ggcgatacgg
     3361 gcaacttgct catacagtgc attcttgatc gttcggttcg tggtcatgga taagagtctc
     3421 tgtgaatccg accgcacggt caagcgcgct cggaatcagc ctccttgacg ttaaatgtcg
     3481 aaccgtatta tattctaacg gtctttagaa tgttagttga taatgatttc tgttgcaagg
     3541 gcgaccaatt gactggaatt ggtcgccatg actgttctag gaggctgaca tgttttttaa
     3601 acaaagggtc aatgaggatg cgtcgatttc atacctgttt gggtgtggtg ggtatggcga
     3661 agcgattgcg gtcgacgtcc tggcgggcga tgaggcgtgg tatatcaagg aagcgcgtaa
     3721 gctgaatgtc gccatcacca cggtgttcga cacccatata cacgccgatc atctttccgg
     3781 cggtcgcaag ctcgccgatt tcgttggcgc tgcctatgcg ctgcatgaga gcaataaggg
     3841 gaaaaccggt tacgcgttta cgccggttgc cgatcacgag atgattgttg ccggtaatac
     3901 gcgtgcgcaa gtcttgcata cccccggcca cacggaagac agcatctgct tgctggtgtc
     3961 agattttcgt cgtggcgaag cgccttggtt cgtattgacc ggcgatacgc tcttcgtggg
     4021 ctcggtcggg cgccccgact tggcggggcg cgaaaaggaa atggctgcgc acctctggca
     4081 gagcctgcgt gaacgcttgc tgacgttgcc ggctgatttg gagatttttc ccggccatca
     4141 agcgggaagt gcctgcggcg cggatatctc tggcaagccg tcgtcgacca ttggtttcga
     4201 aaagcgatgg aatccattac tttctttgga taaggaagag tttatttccg cagtcacggc
     4261 gagtattcca ccgcggcccg gtgatatgga gcatcttgtc gccgccaatc tcggcctaca
     4321 ggccttagtg taaaagagag atcatattca tgtcctaccg aagcgttgcc aagattggtg
     4381 tagagctcgg tttgcgtgcg aactggcgtc agttcagctt attggtgctg gttaatgctt
     4441 tcgtcggcgc gatggtcggt atggaacgca gcatcttacc cttgatcgcc gaacaggaat
     4501 tcaagctggc cgcgcacacc gcgatccttg cattcatcgt tgtttttggc gtttcgaaag
     4561 cgcttaccaa ttatttcgct ggccgattct ccgatgttta tggccgtaaa atcatcctgg
     4621 tcgccggctg gctgatcgcc acgccggtgc cgtttctact gatgtgggcg ccatcgtgga
     4681 attgggtgct gctggcaaat gtattcctcg gcattagcca ggggctgaca tggtccacga
     4741 ctgtgatcat gaaaattgac cttgccggtc ccaaaaatcg tggcatggcg atgggcatca
     4801 atgaattttc gggttacttc gccataggcg cggcag
//
LOCUS       DATCXU010000147         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_687305,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000147 DATCXU010000000
VERSION     DATCXU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687305"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1457)
                     /locus_tag="VIR04_03280"
     CDS             complement(171..1457)
                     /locus_tag="VIR04_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY9459804.1"
                     /translation="MPFTANRQFKLHPRMLTRAKGMYYTSAEGHQILDGTAGLWCVNA
                     GHGRQEIIDAISRQTAELDYAPSFQIGHPNSFLAATAVAELMPKGLDRIFFTNSGSES
                     VDTALKIALAYHRARGDGQRTRLIGRERGYHGVGFGGISVGGIPSNRKMYSAALIPGV
                     DHLPHTHDLSKNANSRGQPAWGGEQFADELERIVALHDASTIAAVIVEPVAGSTGVLI
                     PPKGYLQRLRAITEKHGILLIFDEVITGFGRLGAATASEFFGVTPDLIAMAKGISNAS
                     VPTGAVAVKRSVHDAIVESSTGGIEFFHGYTYSAHPLATAAVLATLDIYQREQLFKRA
                     NGLSKKFEDAAHQLENARHVVDVRNIGLVAGIELQPRTGAPGARAAEVFNKCMDKGVM
                     VRYTGDILAISPPLIIEESQIQEIFHTIEEVLKTVD"
     gene            1666..>2120
                     /locus_tag="VIR04_03285"
     CDS             1666..>2120
                     /locus_tag="VIR04_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9459805.1"
                     /translation="MVFFDPVRDRTGAPTLVDQVANALQEAIRTQLLRPGMSIPSIRE
                     FARSHGLSTFTVAAAYNRLVAQGLLAARPGSGFRVARPEMEAPVVAASNWKPPRIGAF
                     WLLADVFADHSIPIKSGCGWLPPEWLNEAGLQQSLRQLARVPSVQIAGYG"
BASE COUNT          415 a          599 c          635 g          471 t
ORIGIN      
        1 agactgatcg ccttgcgagt tcacgcccgc cccgaaacgg ccgttttcct gggaagttcc
       61 ggcaaggccg ggaccgcgcg agtcgagcct ttttacctga acccccgggc gccggctgaa
      121 gcgcgcatat taccttgcga cctagcgcag cagccgatca gccgcggcaa tcaatcaacc
      181 gtcttcagaa cttcttcgat cgtgtggaag atttcctgaa tctggctttc ttcaataatc
      241 agcggtggcg aaatggccag gatatcgccg gtgtaccgaa ccatgacgcc cttatccatg
      301 cacttgttga acacctccgc ggcgcgcgcg ccaggcgcac cggtgcgagg ctgcaattca
      361 attcccgcca ccaacccgat gttgcgcaca tccacaacat ggcgtgcatt ttcgagttgg
      421 tgagccgcat cttcaaactt tttggatagc ccgttcgctc gtttgaatag ctgttcgcgt
      481 tgatagatgt ccagtgtcgc taaaacggca gcggtggcca gcgggtgtgc cgaataggta
      541 tagccatgga aaaattcgat gccgcccgtg gaggattcga cgatggcgtc gtgtacgctg
      601 cgtttgacgg cgaccgcgcc ggttggaacg ctggcgttgc tgatcccttt ggccatcgcg
      661 atcagatcgg gcgttacgcc gaagaactca ctggcggtgg cggctcccaa acgaccaaag
      721 ccggtgatga cttcgtcgaa aatcagcaaa ataccgtgct tttccgtaat ggcccgtagc
      781 cgttgcaaat accctttggg cggaataagc acacccgtcg aaccggccac aggttcgaca
      841 atgaccgctg cgatagttga agcgtcgtgc agcgctacga tgcgttcaag ttcgtcggca
      901 aactgttcgc cgccccacgc cggttggccg cgcgagttcg catttttcga caggtcgtgc
      961 gtgtgcggca aatgatccac tcccggtatc agagcggccg agtacatttt gcgattcgat
     1021 gggatgccac cgaccgagat gccgccgaac cccacgccgt ggtagccacg ctcgcgcccg
     1081 ataagacgcg tgcgctggcc gtcgccgcga gcccgatgat aggccagagc gatcttcagt
     1141 gctgtatcga ccgattccga acccgaatta gtgaagaata tgcgatccag ccccttcggc
     1201 ataagttcgg caacggctgt ggccgccaga aacgagttgg ggtgcccaat ctggaagctg
     1261 ggcgcatagt ccagttcggc cgtttggcgc gatatggcgt cgatgatttc ctgccggcca
     1321 tgaccggcat tcacgcacca gaggccggct gtgccgtcaa ggatctgatg gccttcggct
     1381 gacgtgtaat acataccctt ggcgcgtgtc agcatgcgcg ggtgcagctt gaactggcga
     1441 ttggccgtga acggcatcca gaggttcgaa agattggggg cgggcgtttg gctgtccatg
     1501 aatacctcga aggcaaaatt gtgtaaggtg acctgtcaag attctatttt cggctggccg
     1561 cctggcgatt gaaatataca gttcattaaa agcacgcgaa ccgtaatggc tttacgagat
     1621 aaactgtgca taattctgga actggcctgg ttttcaaggg atgacatggt ttttttcgat
     1681 cctgtgcgcg atcggaccgg cgcgccgacg ctggtcgacc aggtggcgaa cgctttgcag
     1741 gaagccattc ggactcagtt gcttcgaccg ggaatgtcca ttccgtcgat tcgggaattc
     1801 gcgcgttcac atggtttaag cacctttacg gtggcagccg cctataaccg cctggtggcg
     1861 caaggtttgc tcgcagcccg gccgggctcg ggctttcgcg ttgctcgccc ggagatggaa
     1921 gcgccggtgg ttgcggcttc gaactggaag ccgcccagaa tcggcgcctt ttggctgttg
     1981 gccgatgtct ttgccgatca ttcgattcca atcaaatccg gttgcggctg gctgccgccc
     2041 gagtggctta acgaagcggg tttgcagcaa tcgttgcggc aattggcgcg ggtcccatcg
     2101 gttcagattg ccggttatgg
//
LOCUS       DATCXU010000148         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_884918,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000148 DATCXU010000000
VERSION     DATCXU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884918"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /gene="hslV"
                     /locus_tag="VIR04_03290"
     CDS             complement(<1..469)
                     /gene="hslV"
                     /locus_tag="VIR04_03290"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY9459806.1"
                     /translation="MEQFHATTIICVRRGNQVALGGDGQVTLGNIIIKGTARKIRRLY
                     HDKILAGFAGATADAFTLQERFEAKLEKHQGHLMRAAVELTRDWRTDRVLRRLEAMLI
                     VADSEHTLVLTGTGDVLEPEHGIAAIGSGGAYAQSAAAALLNNTDLAPAEIVKK"
     gene            complement(599..1042)
                     /gene="dksA"
                     /locus_tag="VIR04_03295"
     CDS             complement(599..1042)
                     /gene="dksA"
                     /locus_tag="VIR04_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY9459807.1"
                     /translation="MASKTAATNNQSHLLTEEELLAMPDSSYMNAAQLAFFKARLKAL
                     EHEILSNAGATTENLRETQFVPDPADRATIEEEHALELRTRDRERKLLKKVQQSIARI
                     DSGEYGWCDETGEPIGIARLLARPTATLSLEAQERRELRQKLYGD"
     gene            complement(1116..>2066)
                     /locus_tag="VIR04_03300"
     CDS             complement(1116..>2066)
                     /locus_tag="VIR04_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY9459808.1"
                     /translation="DLLIQDSDEQIVELNNGCVCCTVRGDLVRALNDLRTKREAGKID
                     FERIMIETTGMANPGPVCQTFFMDDDVADYFRLDSVITVVDAKHGMQALDTQEEAQKQ
                     VGFADRILVSKKDLVNDVDYQALRSRLLHINPRAPITPVNFGQTDLAAILDVSGFNLN
                     SILDIDPEFLADEHPDAAHDHDHGHDHDHHDGHDHDDEDECGAECSHHHDHHHAHHDD
                     NIGAFLFQSNKAFDPGRLEEFLSGIVQVYGTDLLRYKGILYLKGINRRMLFQGVHMMM
                     GAEPGKPWASTEKPNTKMVFIGRKLPEETFTRGLEQCLVS"
BASE COUNT          364 a          559 c          627 g          516 t
ORIGIN      
        1 acttcttgac gatctcggcg ggcgccaaat cagtgttgtt cagcaatgca gccgcggcgg
       61 attgcgcgta agcgccgccc gagccgatgg ccgctattcc gtgttcgggt tcaagcacgt
      121 cgccggtgcc ggtcagcacc agggtgtgtt cgctgtcggc aacgatcagc atcgcttcca
      181 gccggcgcag cacacggtcg gtgcgccagt cgcgcgtgag ctcgacggcg gcacgcatta
      241 aatggccctg atgcttctcc agcttggcct cgaagcgttc ttgcaaggtg aaggcgtcgg
      301 ctgtggcgcc tgcgaagccg gccagaattt tgtcgtgata caggcggcgg attttgcgcg
      361 ccgtgccttt aatgatgatg ttgcccagtg ttacctgacc atcgccgccc aaggcgactt
      421 gattgccgcg acgcacgcaa ataatcgtgg tggcgtgaaa ttgttccatg aattcttcct
      481 tagaatccct tacttggggc cgaactgtga aagttcaagg ggacgagccc cgggccccgc
      541 ttgatgaggg gcgtcggggc tgctcgtgtg atgggtcccg ttttgccggc gccgggcttt
      601 agtcgccgta gagcttttgg cgcagttcgc gccgttcttg cgcttcgagc gacagggttg
      661 ccgttggccg cgccaatagg cgggcgatgc cgattggttc gccggtttcg tcgcaccagc
      721 cgtattcgcc ggagtcgatg cgcgcgatgg attgctgcac tttcttgagc agtttgcgtt
      781 cgcgatcgcg cgtgcgcagt tccagcgcgt gctcttcctc gatcgtggcg cgatcggccg
      841 ggtcgggcac gaactgtgtt tcgcgcagat tttcggtggt cgcgcccgcg ttgctaagga
      901 tttcgtgttc cagcgctttc agacgggctt taaaaaacgc aagctgagcc gcattcatat
      961 agctggagtc gggcattgcc aacagttctt cttccgtcaa tagatggctt tgattgttgg
     1021 tggctgctgt tttgcttgcc atgatgtgtc cctttcattg tgtcgtgccg gtctcgcaac
     1081 ccgtgtcgtc gacattggtt ttgcgacgtg gttcattatg aaaccagaca ttgttccaag
     1141 ccccgggtga atgtttcctc ggggagctta cggccaataa ataccatttt ggtgttcggt
     1201 ttctcggtgg atgcccaggg tttgccgggt tcggcgccca tcatcatgtg tacaccctgg
     1261 aatagcatgc gtcgattaat tcctttaagg tacaaaatac ctttgtaacg tagtaaatcg
     1321 gttccataaa cttggacgat accacttaaa aactcttcca gacgccccgg gtcgaacgct
     1381 ttattcgact gaaacaggaa ggcgccgatg ttgtcgtcgt ggtgcgcatg atggtgatca
     1441 tggtggtggc tgcattcggc gccgcactcg tcttcgtcgt cgtgatcgtg gccatcgtga
     1501 tggtcgtggt cgtgcccgtg atcatgatca tgcgcggcgt ccgggtgttc gtcagcgagg
     1561 aattccgggt cgatgtccag aatggagttc agattgaatc cgctaacatc cagaatcgcc
     1621 gccagatcgg tctggccgaa attgacgggc gtgatgggcg cgcgcggatt gatgtgcagc
     1681 aggcgtgaac gcagcgcctg ataatccaca tcgttgacca ggtccttttt ggaaaccaga
     1741 atgcgatcgg caaagccgac ctgcttttgc gcttcttcct gagtgtccag cgcctgcatg
     1801 ccatgtttgg catcgacaac ggtaatgacg gaatcaagcc ggaaatagtc ggcgacgtcg
     1861 tcatccatga aaaatgtctg gcacaccggg cccggattgg ccatgccggt ggtttcgatc
     1921 atgatgcgct caaaatcaat cttgcctgcc tcgcgcttgg tgcgcaagtc attgagcgcg
     1981 cgcaccaggt cgccgcgcac cgtgcagcat acgcagccat tgttgagctc gacgatttgt
     2041 tcgtcgctgt cttgtatcag caggtc
//
LOCUS       DATCXU010000149         6421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_764742,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000149 DATCXU010000000
VERSION     DATCXU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764742"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VIR04_03305"
     CDS             complement(<1..649)
                     /locus_tag="VIR04_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY9459809.1"
                     /translation="MTTSKVPIQFKDPALFRTQSYINGKWVDAADSSTFIVDNPVNGQ
                     TIAQVQNLGQAEARLAIDAANKALKAWRARTGKDRAIILRRWFDLIIASTEDLARLMT
                     LEQGKPITESRGEIAYGASFVEWFAEQAKRVTGDIMGSPNKANRMLVLREPIGVCAAI
                     TPWNFPNAMITRKVAPALAAGCTVILKPAEQTPLSALALAELADRAGIPAGVLNII"
     gene            complement(718..1341)
                     /locus_tag="VIR04_03310"
     CDS             complement(718..1341)
                     /locus_tag="VIR04_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY9459810.1"
                     /translation="MSEMKLAADQLREVGWGVSIFGSARIKPDSPYYALSEALGARLA
                     KAQLTVIAGGGPGIMEAANKGAFQAGGKSVGLNIKLPHEASDNSYQTHSLYFNYFNSR
                     KATFFMHSVAYIALPGGFGTLDELFEILTLVQTRKLPRAPIVLMGSEYWAGLLDWIEN
                     KLQAQKLIGPNDLKLLTVSDDVDEVMHVIETFCSDYAEHLESAPAVP"
     gene            1394..4111
                     /gene="polA"
                     /locus_tag="VIR04_03315"
     CDS             1394..4111
                     /gene="polA"
                     /locus_tag="VIR04_03315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY9459811.1"
                     /translation="MKKTLLLVDGSSYLYRAYHAMPDLRNAKGEPTGALYGVINMLRR
                     LLQDYKADYAACVFDAKGKTFRSDIYPAYKEQRPSMPPDLARQIDPIHQAVVAMGWPI
                     LVIPGVEADDVIGTLADRATKSNMHTIVSTGDKDLAQLVNENVELVNTMSGERQDIAG
                     VMKRFGVAPSQIVDFLMLTGDVIDNIPGVAKVGPKTAAKWLAEYSTIDALVANADAIK
                     GAAGINLRAAISNFDLTRRLLTIKTDCDVSAIAMDLSDLAFKPVDRQALIDLYEEYGF
                     RTWLRELTGDPQRVPLQDSRVAPELPEAPAETHYDTILDWPLFEHWLTLIKNAPLVAL
                     DTETTSIDPMRARLVGLSLSVAPGVACYIPVGHRGPDNVTQLPRQEVLERLRPWLEDA
                     GAPKVLHNAKYDAHVFLNAGVALAGITEDTMLQAYVLESHRRVNLQELGERWLGRTGV
                     TFEDLCGKGAKQICFDEVELDKAGYYACEDADFTLQLHNVLRSRIAKEPGLEATYQLE
                     IETSRVLTTMEHNGVRIDAHVLAMQSHEIGRQLHDLEQKAYELAGQPFNLNSPKQLGE
                     ILFQRMQLPVVRKTASGAPSTDEDVLSRLARDYPLPQVLLDYRVLAKLKSTYTDKLPK
                     MIDPDTGRVHTRYAQAAVITGRLASSDPNLQNIPVRTPEGRRVRTAFVATNGQIVSAD
                     YSQIELRIMAHVSGDENLQRAFAQGEDIHRATASEVFGVDLADVSFEQRRSAKAINFG
                     LIYGMGEFGLASALGITRDAAKAYIDRYFARYPGVAEYMDRTRRTAYEQGYVETVFGR
                     RLWFPEMRGAKGPRRAAAERAAINAPMQGTAADLIKMAMVAVQGWLEAQQLKSLMVMQ
                     VHDELVLDVPRAEVDIVAGQLPGLMCNVAMLDVPLVAEVGVGPNWEQAH"
     gene            4187..4975
                     /locus_tag="VIR04_03320"
     CDS             4187..4975
                     /locus_tag="VIR04_03320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY9459812.1"
                     /translation="MPFSSITGAAATTIHTPAQGLRHGLFDMPTFDGSIQAYYAAPQD
                     RTNVPIVLVIQEIFGLHEHIQDVCRRLAHEGYFAIALELYQRQGNVSHFADVSALIKA
                     VVAKVPDEQVMADLDASAQWAASQGADINRLGVTGFCWGGRLAWMYAAHNPKCKAAVS
                     WYGKLTVGHGPLQTHNPIDVAASLHGPVLGLYGALDASIPVTDVERVQAVLRQGNTAS
                     RASELVVYPKAKHAFFADYRPSYRKNDANDGWGRALAWFGRYLA"
     gene            complement(4997..>6421)
                     /locus_tag="VIR04_03325"
     CDS             complement(4997..>6421)
                     /locus_tag="VIR04_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEY9459813.1"
                     /translation="VALTPALAAMLGANVGTTIIVQLLSFNLTALAPIFILIGVWLFR
                     RHQPGRRRDLGRVFIGLGLLLLALYELVALFNPIQHSHLLATLLDALAAEPVIALLLA
                     MLLTWAAHSSVAMVVLIMSLASHGLVAPAVAYALVLGANLGTSINPLLEGVTDGDPAA
                     KRLPLGNLSIRVITCLMTLAVLPWIPALMTYITPDPARSVANFHTLFNVATAIIFLPL
                     LGPYAKLLSRWLPKRIDPNDPSKPVYLDESAHEVPAVALGNAAREALRLADMLQALLN
                     MARAGFLRDNRHRIAEARDINSAVNHLDELIIQYLATLDQENMSQEDHQRLNEVLTFS
                     SHIAYAAGIAATGLLGHATTIHKKGWALNAAQRQELTQCLDRLIRNQRQTAALFVTED
                     IKSARYLAMEKDQFRAIETDAARQQMQKITSGQAASAGMESLYLDILRDIKTINSYLV
                     GAAAYPILAKHNELLPNRLRDNDN"
BASE COUNT         1329 a         1717 c         1944 g         1431 t
ORIGIN      
        1 caatgatatt aagcacaccc gccgggatgc ccgcgcggtc ggcaagttcg gccagcgcga
       61 gtgcggagag cggagtttgt tcggccggct tgaggatgac tgtgcaaccg gcggccagag
      121 cgggcgccac tttgcgcgta atcatggcgt tggggaagtt ccacggcgta atggcggcgc
      181 acacgcctat gggctcgcgc agcactagca tccggtttgc cttgttgggc gagcccatga
      241 tgtcgcccgt cacgcgcttt gcctgctcgg caaaccactc gacgaacgac gcgccatagg
      301 cgatttcgcc gcgtgattcg gtaatcggct taccctgctc caatgtcatc aaccgggcca
      361 gatcctcggt gctggcgatg atcagatcaa accagcgccg caaaataatg gcgcgatcct
      421 tgccggttcg cgcgcgccat gctttcagcg ctttgttggc cgcatcaatg gccaatcgcg
      481 cctcggcctg gcccaaattc tggacttgag cgattgtttg gccgttcacg ggattatcga
      541 cgatgaacgt gctgctgtca gccgcgtcga cccatttgcc attaatgtag gactgggtgc
      601 gaaacagggc cggatcttta aactgaatgg gtactttgga cgttgtcatg gtgtggaatc
      661 ggcttaatca gttaagaaat aggtgagtca cggtaattga ttgtatgtca tggcgtgtca
      721 gggtacagcc ggggcgcttt caagatgttc ggcgtagtcg gaacaaaacg tttcgatcac
      781 gtgcatgact tcgtcgacgt cgtcggaaac agtaagcagt ttcaggtcgt tggggccgat
      841 cagcttctgt gcctgaagct tgttttcaat ccagtccagg agcccggccc aatattcgct
      901 gcccatgagt acaataggcg cgcgtggcaa tttgcgggtt tgaacgagcg tcagaatttc
      961 gaaaagttca tccagcgtgc caaacccgcc cggcaaagcg atgtaggcca cgctatgcat
     1021 gaagaatgtg gctttgcgcg aattgaagta attgaagtac agactgtgtg tttgatacga
     1081 gttgtcgctg gcttcgtggg gcagttttat gttcaggccc acgcttttgc cgcctgcctg
     1141 gaaagcgcct ttgttggcgg cttccataat gccggggccg ccgccggcga tgacggtaag
     1201 ctgggctttg gccagccgcg cgccaagcgc ttcgcttaac gcgtaataag gagagtctgg
     1261 tttaatacgg gcactgccaa aaatactgac gccccagccg acttcccgca gttgatcggc
     1321 ggcaagtttc atctctgaca taatttcggg aatctgcacc gaagctggca tacgaactat
     1381 tttatttatc gtcatgaaaa aaaccttgtt acttgttgat ggttccagct acctgtacag
     1441 ggcgtaccac gccatgcccg atttaagaaa cgccaagggt gaaccgaccg gcgctcttta
     1501 tggtgtcata aacatgttaa ggaggctgtt acaagattac aaggccgatt atgccgcctg
     1561 tgtcttcgac gccaaaggca agacgtttcg cagcgatatt taccccgcgt acaaagagca
     1621 gcggccgtca atgccgcctg acctcgcgcg gcaaatagat cctatacatc aggcagttgt
     1681 cgccatgggc tggccgattc ttgtcattcc cggcgtcgaa gccgacgacg ttatcggcac
     1741 tttggccgat cgcgcgacca agtcaaatat gcatacgatt gtttcgaccg gcgacaagga
     1801 tctcgcgcaa ctggtcaatg aaaatgtcga acttgtcaat accatgagcg gtgaacgcca
     1861 ggatatcgcc ggcgtgatga aaagatttgg tgtggcccca agccagatcg ttgatttttt
     1921 aatgctgacc ggcgacgtga tcgacaatat tccaggtgtc gcgaaagtgg ggcccaaaac
     1981 cgcggccaaa tggctggccg aatactctac gattgacgcg ctggtggcca acgccgatgc
     2041 catcaaaggc gcggccggca ttaatttgcg cgccgcgatt tctaactttg acttgacgcg
     2101 ccgcttgttg acgatcaaga cggattgtga cgtgtcggcg atcgcaatgg atttatccga
     2161 cctggcattc aagccggttg atcgccaggc gctgatcgat ctgtatgaag agtatggctt
     2221 cagaacctgg ttgcgggaac tcaccggaga ccctcaacgc gtgccattgc aagatagccg
     2281 ggtagctcct gaattgcccg aagcgccggc tgaaacgcac tatgacacta ttttggattg
     2341 gccgttgttc gagcactggc tcaccctgat aaaaaatgcc cctctggttg cgctcgatac
     2401 cgaaacgacg tcgatcgatc ccatgcgtgc gcgtctggtc ggcctgtctt tgtcggtcgc
     2461 ccccggggtc gcttgctaca taccggttgg ccatcgcggc ccggataacg tgacgcagtt
     2521 gcctcgtcag gaagtgctgg aacgtctgcg cccctggctt gaggacgccg gggcgccgaa
     2581 ggttttgcat aacgcaaagt acgatgccca cgtttttctt aatgcgggcg tggcgctggc
     2641 cggaattaca gaagacacaa tgcttcaggc ctatgtgctt gagtcgcaca ggcgggttaa
     2701 tttgcaagag cttggcgagc gctggctggg tcgcacaggc gtaacatttg aggatttgtg
     2761 cggcaaaggc gcgaagcaaa tctgctttga tgaggtcgaa ctcgacaagg cgggctatta
     2821 cgcatgcgaa gacgcggact tcaccttgca gttgcataac gttttgcgtt cgcggattgc
     2881 taaagaaccg ggtttggaag caacgtatca gcttgagatc gaaacttcga gagtgctgac
     2941 caccatggaa cataatggcg tacgcattga tgcgcatgtg ctggccatgc aaagtcatga
     3001 aattggccgg caattgcatg accttgaaca aaaggcctac gagctggcgg ggcagccatt
     3061 taacttgaac tcacccaagc aattgggtga aatattgttt cagcgcatgc aactgcccgt
     3121 ggtgcgaaaa accgccagcg gcgctccatc gaccgacgaa gatgtgttga gccggcttgc
     3181 ccgggactat cctttgccgc aggtgctgct tgattatcgc gttctggcga aactcaaatc
     3241 gacgtatacg gataaattgc ccaaaatgat cgacccggat acggggcggg tgcatacgcg
     3301 ttacgcgcag gccgcagtga ttacaggccg cctggcgtct tccgatccca atttgcaaaa
     3361 tattcctgtt cgcacgcccg aggggcgccg ggtgcgcacc gcatttgtgg ctacgaatgg
     3421 tcagattgtt tcggccgatt attcgcagat tgaattgcgc attatggcgc acgtatcggg
     3481 cgacgaaaat ttacagcgcg ctttcgccca gggcgaggat attcaccggg cgaccgcctc
     3541 cgaggtgttc ggcgtcgatt tggccgatgt ttcgttcgag cagcgccgat cggccaaagc
     3601 cattaatttc ggcctgattt acggcatggg cgaattcggt ctggcctcgg ctttggggat
     3661 cacacgcgat gccgcaaagg cgtatattga ccgttatttc gcccgttatc cgggcgttgc
     3721 ggaatacatg gatcggaccc gacgcacggc ctatgagcag ggctatgttg aaaccgtatt
     3781 tggccgccgg ttgtggtttc cggaaatgcg cggcgccaag ggtccgcgcc gggcggccgc
     3841 cgagcgcgcc gccattaacg cgcccatgca gggtacagca gccgacctga taaaaatggc
     3901 gatggttgcc gtgcaaggct ggcttgaggc gcaacagctc aagagcctta tggtgatgca
     3961 ggtgcacgat gaactggtgc tcgatgttcc gcgcgccgaa gttgatatcg ttgcaggcca
     4021 gttgcccggg ctgatgtgca atgtcgccat gcttgacgtt ccgctggttg cagaagtcgg
     4081 ggttggcccc aattgggagc aggctcattg acgccagccc agccttgttt tgcgaaaccc
     4141 tgcggacggg cgatccgcct gttctttttt tgtcaggagc tatctgatgc cgttctcctc
     4201 tattaccggc gccgccgcaa ccactattca caccccggct cagggcttga ggcatggttt
     4261 gttcgatatg ccaacgttcg atggctcgat tcaagcgtat tacgccgccc ctcaggatcg
     4321 gaccaacgtc cccatcgttc tggtcatcca ggagatcttc ggcttgcacg aacatattca
     4381 ggatgtctgc cggcgcttgg cgcatgaagg ttattttgcg atcgcgcttg aactgtatca
     4441 gcgccagggc aatgtttctc attttgccga tgtgtcggcg ctaatcaagg cagtcgtcgc
     4501 caaggttccg gatgaacaag tcatggcgga tcttgacgcc agcgcgcaat gggcggccag
     4561 tcagggggcc gacatcaatc ggctgggggt taccgggttt tgctggggtg gccgcctggc
     4621 ctggatgtat gcggcgcata acccgaagtg caaggccgcc gtgtcctggt acggcaaatt
     4681 gacagtgggc cacgggccgt tgcagacgca taatccaatc gacgtggcgg cgtcccttca
     4741 cgggcccgtg ctgggcttgt atggggcgct tgacgccagc attccagtaa cggatgtgga
     4801 acgggttcaa gcggtgctgc ggcaaggcaa cacggcatcc agggcatccg agcttgtcgt
     4861 ctatcccaaa gccaaacatg cgtttttcgc cgattatcgg cccagctatc gcaagaacga
     4921 tgcgaacgat gggtggggca gggcattggc atggtttggc cgatacttgg cgtagcggac
     4981 ttcaggcggt ccgaattcag ttgtcgttgt cgcgcagacg attcggcaga agctcgttat
     5041 gtttggccag gattggatag gcggctgccc cgaccagata cgaattgatc gtttttatat
     5101 ctcgcaagat atccagatac agcgattcca ttccggccga cgcggcctga cccgacgtga
     5161 ttttttgcat ttgttgtcgt gcggcgtccg tctcaatggc ccggaactga tctttttcca
     5221 ttgcaaggta gcgagcgctt ttgatgtcct cggttacgaa cagtgcagcc gtctggcgtt
     5281 ggttgcgtat cagccggtcc agacattgcg tgagttcctg acgttgcgcg gcgttcaacg
     5341 cccagccctt tttgtggatg gttgtggcgt gccccagcag tcctgtggcg gcaatgccgg
     5401 ccgcataggc gatgtgcgat gagaaggtca gaacttcgtt aaggcgctgg tggtcttcct
     5461 ggctcatatt ttcctggtcg agcgtcgcca gatattgaat gatcagttcg tcaaggtggt
     5521 ttacggcgct gttgatgtct cgcgcttcgg cgatccggtg gcggttgtcg cgcaggaagc
     5581 cggcgcgcgc catgttcaac aaggcttgca gcatatcggc caggcgcagg gcctcgcggg
     5641 cggcgttgcc tagcgccaca gcgggaactt cgtgcgccga ttcgtccaga tacaccggtt
     5701 tggacggatc atttgggtca atgcgtttgg gcagccagcg agacagcaac ttggcgtaag
     5761 gcccaagcaa tggcaaaaaa atgatggccg tcgcgacatt gaacaaagta tggaaattag
     5821 ccaccgaccg ggcgggatcc ggcgttatat aggtcattag tgcaggaatc catggcaaca
     5881 ccgcaagcgt catgaggcag gtgattacgc gtatgctcag gttcccgagc ggcaggcgtt
     5941 ttgccgccgg gtcgccgtcg gtcacgcctt cgagcagcgg gttgatcgag gttcccaagt
     6001 tggcgcccag cacgagggca tacgcgactg cgggggcgac caggccatgg ctggccagcg
     6061 acatgatcag tacgaccatg gctacgctgg aatgcgccgc ccaggtgagc agcatggcca
     6121 ataacaaggc gatcaccggt tcggcggcga gcgcgtcaag cagcgtggca agcaggtgcg
     6181 agtgctgaat tggattaaag agcgccacca gttcgtacaa ggccagcaac agcaaaccca
     6241 ggccaatgaa gacccgccca aggtcgcgcc tgcggcccgg ctgatggcgc ctgaatagcc
     6301 agacgccgat cagtatgaaa atgggcgcaa gcgcggtcag attaaacgac aggagctgca
     6361 cgataatggt tgtgcccacg ttcgcgccca gcatggcggc cagggccggc gttaacgcga
     6421 c
//
LOCUS       DATCXU010000150         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_23991,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000150 DATCXU010000000
VERSION     DATCXU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23991"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VIR04_03330"
     CDS             complement(<1..1604)
                     /locus_tag="VIR04_03330"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY9459814.1"
                     /translation="MQKLENPLIAQMGVQGISEDVLIEKYAKNGEATLEQVQARVASA
                     LADVEQTKNRKKYASQFLWAMQHGFIPAGRINSAAGVGLQTTLINCFVQPVGDSITET
                     VDGKPGIYTALAQAAETMRRGGGVGYNFSAIRPKGARVKGTGSSASGPISYMKVFDQS
                     CETVESAGARRGAQMAVLNVDHPDIREFIAAKQEKGQLNNFNVSVGVTDAFMRAVDAD
                     ENFELTHTAEPTRDLIDAGAFRRDDGKWVYHTVRARDVWDLIMNSTYAAAEPGVLYMD
                     HINRDNNLSYCETIEATNPCGEQPLPDYGCCCLGSLNLTSYVLEPFTENAGFDFDLLG
                     RVTKVAVRMLDNVLTATKWPLEEQAREAAAKRRVGLGFTGLGDALIMLGIRYDTAEGC
                     ELAARIAHELRDNAYEASADLAAERGSFPLLDTEKYLKDGFASRLPDQLKDKIRTQGI
                     RNSHLTSIAPTGTISLAFADNASNGIEPAYSWFYSRLKRMADGSKKEYTVEDHAYRVY
                     RSMGFDVDNLPHAFVSALQIPAIDHM"
     gene            1863..2423
                     /locus_tag="VIR04_03335"
     CDS             1863..2423
                     /locus_tag="VIR04_03335"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY9459815.1"
                     /translation="MGNRLSKIVTRTGDGGTTGLGDGSRVDKDSARINSIGNVDELNS
                     HIGLLLCEDMPPGLAGEMFSIQHDLFDLGGELCIPGYSIIAQTHVARLEALVEKYNAN
                     LPRLKEFILPSGSRAAALAHVCRTICRRAERSIVALGKLETINEPLRQYINRLSDLLF
                     VLSRVLNRHAGGSDVLWDKDRDRDPA"
     regulatory      2551..2709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2845..4620
                     /locus_tag="VIR04_03340"
     CDS             2845..4620
                     /locus_tag="VIR04_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY9459816.1"
                     /translation="MISSSAAAQTAPPVPQLDHVIVTASRTAQLESSVLGDITVIDNQ
                     ELDQAGQSSIAEVLARHPAVQFYNSGGPQTVTGVYLRGAGPSQTLVLVDGIRINSSTQ
                     GGANWNAIDPATIDHIEIIRGAASSLYGSDALGGVINIITRKPHENQAPSAWANMGYG
                     SYGTSKSSLGFSGAERGWDYGLSSSLAQSSGFNASNRDNPNFNSDADGYSQHSLSGAL
                     GYSWAPGHRVGVTALNNYTNGDYDAGELHPAYSITRQQAYTVSSTDDLTSFWQSMWRF
                     GLSKEYVEDRSYHTRYSSLQRSYTWQNNLKLTQDQHLSVVLERLEERPQYSPGFSINR
                     RDTNSAGLVYRGDFGAHHVQASARNDTISGYGNQLTGGLSYQQDLSDQWRVGLAGNTG
                     FRAPTFIDLYSPFAPNPELKPEKSRNIEFNLHYTADSVRLGLAAFQNKIADMITLDPN
                     RNWAAFNIDAATIRGITLTGEKDYGKATLRIGADFMNPRDDQTGNQLARRARQTYRAS
                     VDYRLKSWTVGPEYLFVGKRYDDAANQTSLGGYGLLNVTAAYSVTPNVAIQVRWNNLL
                     NKDYTNAYGYNAPGSNVFINVAWRL"
     gene            4630..>5031
                     /locus_tag="VIR04_03345"
     CDS             4630..>5031
                     /locus_tag="VIR04_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459817.1"
                     /translation="MHRCKSIVLCIALFFCLSAPAGLFAAQTRNPGADQIKQRVITLA
                     PHITELIFAAGAGGRIVATVDSSTYPAAARSIPRIGDGVSVSVEKVVASRPDLVVAWM
                     PSNATRMLMPALTGLHVPLIYSSPRKMADIPP"
BASE COUNT         1107 a         1409 c         1376 g         1139 t
ORIGIN      
        1 aacatgtgat caatggcggg tatttgcagc gccgagacga aagcgtgggg cagattgtcg
       61 acgtcgaacc ccatggagcg ataaacccga taggcgtggt cttcaactgt gtactctttt
      121 ttactgccat cggccatacg cttcaaacgg ctgtagaacc aggaatatgc cggttcaatg
      181 ccattggacg cattgtcggc aaaggcgagt gaaatcgtgc cggtcggcgc aatcgatgtt
      241 aaatgcgaat tgcgtattcc ctgggtccgt atcttgtctt ttaactgatc gggcaagcgg
      301 gatgcgaacc cgtccttcag atacttttcg gtatccagaa gcgggaagga accgcgctcg
      361 gcagccaggt cggcggaagc ctcgtacgca ttgtcgcgca gttcgtgcgc aatccttgcg
      421 gcgagttcgc agccttcggc cgtgtcgtat cgtatgccca gcatgatcag cgcatcgccc
      481 agacccgtga accccagccc gacgcgacgc ttggcagccg cttcacgcgc ttgttcttca
      541 aggggccatt tcgtggcggt caggacgttg tcaagcatgc gcacggcgac ttttgtgacg
      601 cggccaagca agtcgaaatc aaaacctgca ttttccgtga acggctcgag cacataagac
      661 gtcaaattca gactgccgag gcagcagcaa ccataatcgg gcaagggctg ctcgccgcat
      721 gggttggtgg cctcgatcgt ttcgcagtac gacagattgt tgtcgcgatt gatgtggtcc
      781 atgtacagca cgccgggttc ggcggccgca taggtgctat tcatgatcag gtcccacaca
      841 tcgcgcgccc gcaccgtgtg gtagacccac ttgccgtcat cgcggcggaa ggcgcctgcg
      901 tcgataagat cgcgggttgg ttcggcggtg tgtgtcagtt cgaaattttc atccgcatcg
      961 actgcgcgca tgaacgcatc ggtgacgcca accgacacgt tgaagttgtt caactgaccc
     1021 ttttcctgct tggcggcaat gaactcgcgg atgtcggggt gatcgacatt gagcaccgcc
     1081 atctgggccc cgcgacgggc gccggcggat tcgaccgttt cgcaggattg gtcgaatacc
     1141 ttcatgtagg aaattggccc cgacgcgctg ctgcctgtgc ccttgacccg ggcccctttg
     1201 gggcggatcg ctgaaaagtt gtagcccacg ccgccaccgc gtcgcatggt ttctgctgcc
     1261 tgcgccaaag cggtgtaaat cccgggcttg ccgtcgacgg tttcggtaat cgagtcgccc
     1321 acgggttgaa cgaaacaatt tatcagcgtg gtttgcagcc ccacacccgc cgcgctgtta
     1381 atgcggccgg ccggaataaa gccatgctgc atggcccaca gaaactgact ggcatatttt
     1441 tttctgtttt ttgtttgctc aacatcggcc aaagcgctgg caacgcgtgc ttgcacctgc
     1501 tccagggtgg cttcgccatt tttagcgtat ttttctataa gtacgtcttc ggatatgcct
     1561 tgaactccca tttgagcgat caaaggattt tccagttttt gcattgcaat tcctcttgcg
     1621 gccagcaggg ctttgcgccc tttcgcaacc gtcattttaa ttttaaataa gacgccgaca
     1681 atatgtcggc caaacacacg ccatccagat taaatcatta aatcagttta ggacaattca
     1741 ccggttgata ataaacaaat tcacaagttg aactggctgg tctgttacga tttacttcat
     1801 ggcatttggt ttgcttttgt gatactagta tctgcattct cactcatttt tttatccaga
     1861 atatgggcaa tcggctttca aaaatcgtaa ctcgcacggg cgatggcggc accacggggc
     1921 taggcgacgg cagtcgtgtc gacaaggaca gcgcgcgcat aaattccata ggcaatgtcg
     1981 atgagctgaa ctcgcatatt ggcctgttgc tgtgcgagga catgcctccc gggctggccg
     2041 gcgagatgtt ctccattcag catgacctgt tcgacctggg cggagagctg tgcattcccg
     2101 gctatagcat cattgcgcaa acgcatgtgg cccggctgga agcgctagtg gaaaaatata
     2161 acgccaattt gccccggctg aaggaattta ttttaccgtc gggttcacgc gcggccgctt
     2221 tggcgcatgt ctgccgcaca atatgccgcc gggcggaacg cagcatcgtc gcgctgggca
     2281 agcttgaaac gattaacgaa ccgttgcgcc agtatataaa tcgattatct gacctgttat
     2341 tcgtgctctc ccgggtattg aatcgccatg cgggtgggag tgacgtgtta tgggacaagg
     2401 accgcgatcg cgacccggcg taatgcggca aatgcgcgtg ggcaacaaca atcaaagcag
     2461 ggttttcggc cttccagcgt aatgccctgt gctatatttt gcgtgacaga ggtgctttcg
     2521 ggacgaaaaa cgcaaaccac tgttactgcg tcgccgcact agcgatatcg tcctgcgaaa
     2581 gttaaacggg aaacagaacc cccaagacgt atgcaaatgc gtcttgctgt ataaactgtg
     2641 ctgcccccgc aacggtaagc atgtcttgca catgccagcc cgacaccggc cctgatcaaa
     2701 gttcatgcat ggcaatgcgc ggcggtttat ttcgcggcgc ccagcgatgc ttcattcacc
     2761 catagtgtgc ggggacgcgc actatgatca ggtcatacga tgcattccaa atccttccgg
     2821 cgcccgcttg tcttttcatt ttgtttgata tcatcctccg cagccgctca aaccgcccca
     2881 cccgttcctc aactcgacca tgtcatcgtc actgcaagcc gtacggcgca gttggagagc
     2941 agtgtgctgg gcgatattac ggtcatcgac aaccaggagc ttgatcaggc gggccaaagc
     3001 agcattgccg aggtgctggc ccggcatccc gcagtccagt tctataacag cggcggtccg
     3061 caaactgtaa ccggcgttta tcttcgaggc gcgggaccaa gccagacgct ggtgctggtc
     3121 gacggaattc gcatcaatag ctccacacag ggcggcgcca actggaatgc catagatcct
     3181 gcgacgattg accacatcga gattattcgc ggcgccgcaa gcagcctgta tggctccgat
     3241 gctctcgggg gtgtaatcaa catcatcacc agaaagccgc atgaaaatca agcaccgagc
     3301 gcctgggcca acatgggcta cggctcctat ggcacatcca aatcaagctt gggtttttcc
     3361 ggcgccgagc gcgggtggga ttacggcctg tccagcagcc tcgcgcaaag tagcggattt
     3421 aatgcaagca acagggataa tcccaatttt aattctgatg ccgatggcta cagccagcat
     3481 tcgttatccg gtgccctggg ctatagctgg gccccgggcc atcgagtcgg cgtaaccgct
     3541 ttgaacaact acacgaacgg cgattatgat gcgggcgaac ttcatccggc ctactcgatc
     3601 acacggcaac aggcctacac agtgagcagt accgacgatc tgaccagctt ttggcaaagc
     3661 atgtggcgtt ttggcctgag caaagagtat gtcgaagacc gcagctatca cacccgttac
     3721 tccagtcttc aacggtctta cacctggcaa aataacctga agctcacgca agaccagcac
     3781 ctttccgtcg ttttggagcg cctggaggaa aggccgcagt attcgcctgg cttctccatc
     3841 aaccggcgcg acacaaactc ggccggattg gtttatcgcg gcgactttgg cgcgcatcac
     3901 gtccaggcga gtgcgcgtaa tgacaccatc tcggggtatg gaaatcagct taccggagga
     3961 ctctcgtatc aacaggacct gagtgatcag tggcgcgtgg ggctggcagg caacacggga
     4021 ttcagggcgc cgacttttat tgatctctat agccctttcg ctccaaatcc cgaacttaag
     4081 cccgaaaaat ccaggaatat agaattcaat ctgcattaca cggctgattc ggtccggttg
     4141 ggtctggcgg cgtttcaaaa caagatcgcc gacatgatca cgctggatcc aaaccgaaac
     4201 tgggccgcct tcaatatcga tgcggcaacg attcgcggca ttacgcttac cggcgagaaa
     4261 gactacggca aggctacttt gcgtatcggc gcggatttca tgaacccgcg cgatgatcag
     4321 accggtaatc agttggcgcg gcgcgcgcgc cagacctatc gtgccagcgt tgattaccgg
     4381 ctgaaatcgt ggacggttgg ccctgaatat ttgttcgtcg gcaaacgcta tgacgacgca
     4441 gccaaccaga caagcttggg aggatatggg ttattgaacg tgacagccgc ctatagcgtc
     4501 acacccaacg tggccattca agtacgctgg aataaccttt taaacaagga ttacaccaat
     4561 gcatacggct acaacgcgcc aggctccaat gtgttcatca atgtggcgtg gcgcttgtaa
     4621 gagccgatga tgcatcgctg caaaagcatt gttctatgca tcgccctgtt tttttgcctg
     4681 tcggccccag ccggattgtt tgctgcgcaa acgcgaaatc ccggcgcgga tcaaataaaa
     4741 caacgggtta taacgctggc ccctcatatt actgaattga tttttgccgc tggcgctggc
     4801 ggccggatcg tggcgacggt cgatagcagc acctaccccg ccgccgcgcg atccatcccc
     4861 cgaataggcg acggcgtcag cgttagcgtt gaaaaagtgg tcgcttccag gcctgacctg
     4921 gtcgtcgcgt ggatgccttc aaatgcgacc cgaatgctga tgccggccct gacggggctg
     4981 catgttccgc tgatctattc cagtccccgg aaaatggcgg atattccccc a
//
LOCUS       DATCXU010000151         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1119040,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000151 DATCXU010000000
VERSION     DATCXU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119040"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VIR04_03350"
     CDS             <1..349
                     /locus_tag="VIR04_03350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY9459818.1"
                     /translation="KRSVIINGLSGLCVTKLDVLDGVEEIKIGVGYRYKGEFLDVFPY
                     GAHAAAEAEPVLEEMPGWSESTVGITDYDKLPVNARRYLERIAEICEVPIDMVSTGPD
                     RLETIVLHHPFNG"
     gene            398..940
                     /locus_tag="VIR04_03355"
     CDS             398..940
                     /locus_tag="VIR04_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY9459819.1"
                     /translation="MNLPPSTERDHWISWEQYHALIERLALTIHESGWKFDKLVCLAR
                     GGVRVGDVLSRIFDLPLGILSTSSYREAAGTQQGQLDIAQFITITRGTLDGRVLLVDD
                     MVDTGLTFSRVREHLLTQFPAITELRSAVLWWKGHAQAAPDYFVEKLPTNPWIHQPFE
                     DYDSIRPHQLEAWVRKGVRN"
     gene            1014..1226
                     /gene="rpsU"
                     /locus_tag="VIR04_03360"
     CDS             1014..1226
                     /gene="rpsU"
                     /locus_tag="VIR04_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY9459820.1"
                     /translation="MPIVRLKENEPFEAALRRFKRTIEKNGLLTELRSREFYEKPTAE
                     RKRKHAAAVKRHYKRIRSQQLPPRMY"
     gene            1230..>2571
                     /gene="dnaG"
                     /locus_tag="VIR04_03365"
     CDS             1230..>2571
                     /gene="dnaG"
                     /locus_tag="VIR04_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY9459821.1"
                     /translation="MIPEPFVQDLLARTDIAEVVGRYVPLRKGGANLIGLCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGSAITFLIEHTGASFPEAVHTLAANIGMVVPEEPRSPQ
                     QRAASKRYKQEVSRQEQILGLAQAHYVRELKASPAAIAYLKQRGLSGEIAGHFGLGWA
                     GADRRGLAAVFPEYEDPALIESGLVIEAEDGRRYDRFRERVMFPIRNARGKLIGFGGR
                     IIGKGEPKYLNSPETSLFSKGHELYGLWEARSAIRTEGHVLVVEGYMDVVSLAQQGVA
                     NAVATLGTATTSEHIQKLLRTSDKIIFSFDGDTAGRKAAWRALQTGLPLLRDDVSIRF
                     LFLPPEHDPDSYVRALGAAAFRATAAEAVPLSRFMLDELVSRHAMSEAEGRAACVYEA
                     KPLLALLPEATLRIQIEHEFAKRVQLTPEELAQMLAAPAPKLARRAPGVAPEGRQ"
BASE COUNT          538 a          699 c          757 g          577 t
ORIGIN      
        1 gaaacgctcg gtcataatca atgggctttc cggcttgtgc gtgaccaagc tggacgtgct
       61 tgacggcgtc gaggaaataa aaatcggggt tggctatcgt tataaaggcg aatttctgga
      121 tgtgtttccg tatggggcgc atgccgccgc cgaagccgag cctgtgcttg aagagatgcc
      181 gggctggtcc gagtcgacag ttggcataac cgactacgac aagttgcctg ttaatgcgcg
      241 ccgttatctg gagcgcatcg ccgaaatctg cgaggtgcct atcgacatgg tttcgaccgg
      301 accagaccgc cttgaaacaa tcgtgctgca tcaccccttc aatggatgat gcaattaaac
      361 agcgcaactg aaaaaagtca ttacaggaag tactttcatg aatctgcctc cgagcaccga
      421 gcgcgatcac tggataagct gggagcaata ccatgctctg attgagcgcc tggcgctgac
      481 tattcatgag tctggctgga agttcgacaa acttgtgtgc cttgcccgtg gcggcgttcg
      541 cgtgggcgac gtgctgtcgc gcatctttga tttgccgctg ggcattttgt cgaccagcag
      601 ctaccgcgag gcggcgggaa cccagcaggg gcagcttgat atcgcccagt tcattacgat
      661 tacccgcggc accctggacg ggcgagtgtt gctggtggac gacatggtcg ataccgggct
      721 gacattcagt cgtgtccgcg aacaccttct gacacaattt ccggccatca ccgaacttag
      781 aagcgcggta ctgtggtgga aaggccatgc ccaggcggcg cccgattatt ttgtcgagaa
      841 attgccaacc aacccatgga tccatcagcc tttcgaggat tacgacagta ttcggccgca
      901 ccagctcgag gcctgggtaa gaaagggcgt tcgcaattag ccgtcttatg ctagaattac
      961 cggttactgt tttaacttac ttttattttt taacatacaa ggcttaggac tacatgccta
     1021 tcgttcgtct gaaagaaaac gagccctttg aagccgcgtt gcggcgtttt aagcgcacta
     1081 ttgaaaagaa tggtctattg accgaattgc gttcgcgcga attctatgaa aagccgactg
     1141 ccgaacgcaa gcgcaagcat gccgcggccg tcaaacgcca ttacaagcgc attcgcagtc
     1201 agcaattgcc tccacgtatg tattgatcat tgattccaga gccatttgtt caggatttgc
     1261 tcgcccgaac tgatattgct gaagtcgtcg gacgatacgt tccgctgcgc aagggcgggg
     1321 ccaacctgat tggcctgtgc ccatttcata acgaaaaaag cccctcattt accgtaagcc
     1381 ccaccaaaca gttttaccac tgttttggtt gcggcgccca cggtagcgcc attacgttcc
     1441 tgattgagca cactggggcc agcttcccgg aggctgtgca cactcttgcc gcaaacatcg
     1501 gcatggtcgt gcccgaagag ccgcgtagcc cgcaacagcg cgcggcatcc aaacggtata
     1561 agcaggaagt ctcgcgccag gaacagattt tgggtctggc gcaggcgcac tatgtgcgcg
     1621 aattgaaggc atcgccggcg gcaattgctt atttgaagca acgtggctta agcggtgaaa
     1681 tcgccgggca ctttggcttg ggctgggctg gcgccgaccg gcgcggactg gcggcggtgt
     1741 ttcccgagta cgaagatccc gctctgatcg agtccggcct ggtgatcgaa gccgaggatg
     1801 gccggcgcta cgaccgcttt cgcgagcgtg tcatgtttcc gatccgcaat gcgcgcggta
     1861 agcttattgg ttttggcggg cgcattattg gcaagggcga gcccaagtac cttaattcgc
     1921 ccgaaacgtc tcttttttcc aaaggccatg aattgtatgg tttgtgggag gcacgctcgg
     1981 cgatacgcac ggaagggcac gtgcttgtcg ttgagggata tatggacgtt gtcagcctgg
     2041 cgcaacaggg ggtcgccaat gcggtggcca cgctgggcac ggcgacgaca tccgaacata
     2101 tccagaagct gttgcgcacc agcgacaaaa ttattttcag cttcgatggc gataccgcag
     2161 gccgcaaagc agcctggcgg gcgcttcaga caggtttgcc attgttgcgc gacgatgttt
     2221 cgattcgatt cctgttcttg ccgcccgagc atgatcccga ttcttatgtg cgcgcgcttg
     2281 gcgctgcggc gtttcgcgcg acggcggccg aagccgtgcc cttgtcccgt tttatgctcg
     2341 atgaactcgt gtcgcgccac gccatgagcg aggccgaggg acgcgcggcg tgcgtttacg
     2401 aggcaaaacc tttgttggcg ctgttgcccg aggcaacgct gcggattcag atcgagcacg
     2461 aatttgccaa acgtgtgcaa cttacgcccg aagagctggc gcaaatgctg gcggcgcccg
     2521 cgccaaaatt ggcgcgtcgt gcgcctgggg ttgcccctga gggccggcag c
//
LOCUS       DATCXU010000152         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_333228,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000152 DATCXU010000000
VERSION     DATCXU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333228"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VIR04_03370"
     CDS             <1..618
                     /locus_tag="VIR04_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEY9459822.1"
                     /translation="WEAAVHPRAAGPFVRAHDIRRDLVVGQWALIPHFAKSAKLPYQT
                     NNARSEELASKASYRRSWQRGQRCIIPAMSFDEPCWETGRNVWWQFRRSDGAPWGLAG
                     LWNTWTDPATGEVLESYTMLTINADAHALMRRMHKPDPKKLAVAQDKRSVIPIEVNDI
                     DCWLTGSVEEAYSLLRLAPEHIFDAKPIETPSPDGADAITQQTLW"
     gene            complement(807..1336)
                     /locus_tag="VIR04_03375"
                     /pseudo
     CDS             complement(807..1336)
                     /locus_tag="VIR04_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            1275..1736
                     /locus_tag="VIR04_03380"
     CDS             1275..1736
                     /locus_tag="VIR04_03380"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY9459823.1"
                     /translation="MNSLELAIPPPIVMLVTALFMWLLSVFLPGFTLHALHSAAGAVI
                     IGLAGLSVGMAGVIAFRRAQTTLDPRTPGSASALVTSGIYRLTRNPMYLGILMMLIAW
                     ALFLGNALSLLFAFAFALYIHHFQIRPEERFLQEKFGADFASYKSRVRAWV"
     gene            1896..2327
                     /locus_tag="VIR04_03385"
     CDS             1896..2327
                     /locus_tag="VIR04_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459824.1"
                     /translation="MKNAFLAAVLLTVAIPASAQVGVSVNIGEPGFYGQISIGNAPPP
                     VLIYPQPVVIAPAPRYVGVPPVYLRVPPGQAKHWRNYCARYDACGRPVYFVRDDWYLN
                     RYVPYYHDRHGDRHEYRRHDDRRDRGDRGDHDRGRGHGHDR"
     gene            2409..>2518
                     /locus_tag="VIR04_03390"
     CDS             2409..>2518
                     /locus_tag="VIR04_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459825.1"
                     /translation="MQTRIVHLGAGAFHRSHQAMYTDQLMSSGDTRWGITA"
BASE COUNT          522 a          726 c          694 g          576 t
ORIGIN      
        1 tgggaggccg ccgttcatcc tcgtgccgcc ggcccttttg tccgggcgca cgatatcagg
       61 cgcgatctgg tggtcggcca gtgggcgctt atcccgcact ttgccaagtc cgcaaaatta
      121 ccataccaga cgaataacgc ccgttcagaa gaattggcca gcaaggcaag ctatcgccga
      181 tcctggcagc gcggccagcg ctgcattatc ccggcgatgt ctttcgacga gccttgctgg
      241 gaaaccggcc gcaacgtatg gtggcaattc cgaaggtcag acggcgctcc ttggggtctg
      301 gccggtctgt ggaatacttg gaccgatccg gcaacgggcg aagtactgga aagctacacg
      361 atgctcacga tcaacgccga tgcacatgcg ctaatgcgcc gaatgcacaa accagacccg
      421 aagaaactgg ccgttgccca agacaaacgc tcagtcatcc cgatcgaagt gaatgatatc
      481 gattgctggc tgacgggctc ggttgaagag gcgtacagcc ttttgcgcct ggcgccagaa
      541 catattttcg acgccaagcc catcgagacg ccttcgccgg acggcgccga cgccatcacc
      601 cagcagacgc tgtggtagct attccggcat tttctgtcaa cgcccgtttt accaaacgtc
      661 gaactttttg cctaagacca cgaattacta cgcacacggg caggtaagga tgaagaatct
      721 ccactatttc gactgtacgt acatctgctt ttgcgactgc cccgatttcg ttcgttcatc
      781 agtcgcaaaa agatctgggt tggctttcac gaggtctgaa agtttcttgt acccatagac
      841 tcggggatgg tgaaatcact atcgctggtt accaggcaaa acccgtcgaa ccgacgcgtg
      901 tagagcaggt ccatagcatc aataatcaac gcactgtcgg tagagtcttg ccagtcgtat
      961 aggcgaattg ttggacgggc ttgatggcat atttctgaag gacatttttc cacgacgcac
     1021 tgtgcgacga tgtaaaatcg ccatagatac gtttgacaga agcctcacca aaccgggcaa
     1081 cttcagccaa gatgccttct atgacggttg cttgagcatt gtcggcatcg atgagtaccg
     1141 cgaatctggc tgcaagctcg tcttcgtgct gagtttttcc catcgcgcgt gaggttccca
     1201 ttctgatttc tcctattgag gatgcgtcgc caaagcacaa cacaactcca agagaatact
     1261 cccggaacgc aagtatgaat tcccttgaat tggccattcc gccacccatt gtcatgctcg
     1321 tgactgccct gttcatgtgg ctgctttccg tctttctgcc tggattcacg ctgcatgcgc
     1381 tccacagcgc cgcaggagcg gtcattattg gtctggccgg cctgagcgtc ggcatggcgg
     1441 gcgtcatcgc cttccgtcgt gcgcaaacca ccctcgaccc aagaacgccg ggttctgcat
     1501 cggcattggt cacatccgga atttatcgcc ttacccgcaa cccgatgtac ctgggaatac
     1561 tgatgatgct gatcgcttgg gcgctattcc tcggcaatgc attgtcgctg ctatttgcat
     1621 tcgccttcgc gctttatatc caccattttc aaattcgccc ggaagagcga ttccttcagg
     1681 aaaaattcgg cgccgatttt gcgtcatata agtcgcgggt ccgagcgtgg gtatagtcgc
     1741 gtgcagcatc cactttcgct atgcaggcct tcggcagcaa tcgatatgac cgaggagtgg
     1801 gttgcggtga gccgagtccc cacgcatgtt gcaagtcgca tgcagtttcg gtacagtgac
     1861 cgggtgggga aggaattttc ggagcttttc gtactatgaa gaacgcattt ctagccgctg
     1921 tcctgctgac cgtggcgatt cctgcaagcg cacaggttgg cgtgtcggtc aatattggcg
     1981 agccgggttt ttacgggcag atctccatcg gaaacgcgcc cccgccagta ctgatttatc
     2041 cccagccggt agtgattgcg ccggcgccgc gttatgtcgg cgtaccgcct gtttacctgc
     2101 gcgtccctcc cggtcaggcc aagcattggc gcaactactg cgccaggtac gatgcctgtg
     2161 gccgcccggt ctactttgtc cgggacgact ggtatctaaa ccgctatgtg ccgtactacc
     2221 atgaccggca cggcgatcgt cacgaatatc gcagacatga tgatcgtcgc gaccgtggcg
     2281 accgtggcga ccatgatcgc ggccgcggac acgggcacga tcgctagcga ttgccaaaca
     2341 gactttccaa tgaggcgttg agtgggctga ggccgggcgt cgcgattcct ggttacgacc
     2401 gttcgcgcat gcaaactcgc atcgtgcatc tgggcgcggg tgcgtttcat cgtagccacc
     2461 aggcgatgta caccgaccag ctcatgagta gcggcgacac gcgatggggc attacggc
//
LOCUS       DATCXU010000153         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_668180,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000153 DATCXU010000000
VERSION     DATCXU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668180"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..678)
                     /locus_tag="VIR04_03395"
                     /pseudo
     CDS             complement(1..678)
                     /locus_tag="VIR04_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(779..1327)
                     /locus_tag="VIR04_03400"
     CDS             complement(779..1327)
                     /locus_tag="VIR04_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY9459826.1"
                     /translation="MNTPFPGFSTPSAGTEAPLEMLSACHIRIEHQCATLKRLAAHLT
                     EHGADDQARQAAANILRYFDTAAPQHHADEEENVFPALIESMAGSDAVCIHQMIEGLT
                     ADHRALEAGWRGLRRALERITEGQTAELPASELAKFVELYERHLKIEDDDLLPMAARL
                     LADDELDHIGQAMRKRRGIDAL"
     gene            1438..1965
                     /gene="thpR"
                     /locus_tag="VIR04_03405"
     CDS             1438..1965
                     /gene="thpR"
                     /locus_tag="VIR04_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY9459827.1"
                     /translation="MKRIASVKSHRLFFALWPDGATSQSLLRLQSGVRGRKTHRDDFH
                     ITLAFLGERPADLLPALKEILAGLGRPEMTLVLDRLGYFRHNRIAWVGMGTPPPALFQ
                     LHDTLLRELTNRSISLSAYGDFRPHITLARNADAPAEQPFAPITWLACRLVLAESMPA
                     FDGARYRLLAEVLPA"
     gene            complement(2006..>2343)
                     /locus_tag="VIR04_03410"
     CDS             complement(2006..>2343)
                     /locus_tag="VIR04_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY9459828.1"
                     /translation="AASEADFDGFIPDACLINRYEPGTRLSLHQDKNERHFDAPIVSV
                     SLGIPATFLFGGHERTDKTVRVLLLHGDVVVWGGSDRMRYHGILPIKNAYHPLIGGRR
                     INLTFRKAG"
BASE COUNT          435 a          649 c          701 g          558 t
ORIGIN      
        1 gggtacacga atacgctggc aaacgaggta tggcgtccgt tggccgagtc aaacgggctc
       61 ttgcgaacca gccggtgaat gcccgtctcg gtgcgtaaaa agccgaaggc ataatcacct
      121 tcgattttta tcgtggccga cttgatgccg gcgacttcgc cgtaggattc ttccaatacc
      181 tgggccttga agtctttgtg ctcgcaatat cgcaggtatt ggcgtaacaa catcgaagcc
      241 cagtcctgag cttcggttcc acctgcgccc gactggatat ccaggaagca attcaacggg
      301 tcggcagggt tggagaacat acgccggaac tcaagggttt cgagccgttt gcccatgact
      361 tcactgtccc gctcgatcgc gcttagagtg gcgtcgtcgt gatcgccggc ggccagttcg
      421 aatagttcct gcgcgtcggt caggctggtt tgcagcctgg tcagcgtttg aacgacgttt
      481 tcaagacttt ttttctcgcg gcccaactcc tgggcacgtt tggggtcgcg ccacacttca
      541 gcgctttcga gttcggcgtt tacaacctgc agccgttcgg atttggcatc gtagtcaaag
      601 atacccccgt agagccagtt ggcgctcctc gtagtctttc aggcggtgag caagctggtt
      661 ttggcgttcg gcttccataa tattccaagg tcggaggttt tattatcggg ttcaaatgct
      721 tattttaacc cgacattgcg gccggcccgg gttgcggcgc gcctgcgcgc ccggatcgtc
      781 aaagtgcgtc gatgccccgg cgcttgcgca tggcctggcc aatgtgatcc agttcgtcgt
      841 cggcaagcaa gcgggccgcc atcggcaaaa gatcgtcgtc ctctatctta agatgacgct
      901 cgtacagttc gacgaacttt gccagttccg atgccggcag ttcggcggtt tggccttcgg
      961 tgatccgctc aagcgcgcgc cgcaggccgc gccagcccgc ttccagcgcg cggtggtcgg
     1021 cagtcaagcc ctcgatcatc tggtgtatgc agacggcgtc cgacccggcc atcgattcaa
     1081 ttaatgccgg aaaaacgttt tcttcctcgt cggcatggtg ctgtggcgcg gcggtatcga
     1141 agtaacgcag gatattcgcg gcggcctggc gcgcctgatc gtcggcgccg tgttcggtca
     1201 gatgggccgc aaggcgtttc agggtcgcgc actgatgttc gattcggatg tggcaggccg
     1261 acagcatttc aagcggcgcc tcggttccgg ccgacggtgt ggaaaagcct ggaaaggggg
     1321 tattcattgc ggcaggctcc tgacctgata ggatggtatt gattttgcta cattactacg
     1381 ctgggtcgtt gcgcggcaag cctcgccgtt cagtgggtga gccgacggag agaacctgtg
     1441 aagagaatcg cgtcagtgaa atcgcatcgt ctttttttcg ctctgtggcc tgatggcgcg
     1501 acgtcacaaa gcctgctgcg acttcagtcg ggggtgcgcg ggcgaaagac ccatcgtgac
     1561 gattttcata ttaccctggc attcctgggt gagcgtcccg ctgatttgct gccggccttg
     1621 aaagaaatac tggctggatt agggcggcct gaaatgacgc ttgttctcga tcgattgggt
     1681 tactttcgtc acaatcgaat cgcctgggta ggaatgggaa cgccgccgcc cgcgcttttt
     1741 caattgcacg atacgctgct gcgcgaattg acgaaccgtt caatttcact ttccgcctac
     1801 ggtgatttca ggccgcatat tactttggcg cgcaacgccg acgcgcctgc tgagcaaccg
     1861 tttgcaccga tcacatggct tgcttgccgg ttggtattgg cggagtcaat gccggctttc
     1921 gacggagcgc gatatcgcct gctggccgaa gtcctgccgg cttaagccgc ctgtacacta
     1981 attttgcgcc gcatttgcat gggggttagc ccgctttgcg aaatgtaagg ttgattcggc
     2041 gcccgcctat gagcggatga taggcgtttt taatgggcag tatgccgtgg taacgcatgc
     2101 ggtcggatcc accccagact actacatcgc catgcagcaa cagtacgcga acagttttgt
     2161 cggtgcgctc gtggccgccg aatagaaacg ttgccggaat gcccagcgac acggagacaa
     2221 taggcgcgtc gaaatgtcgt tcatttttgt cctgatgcag cgacaaccgg gttcccggct
     2281 cgtatcggtt aatcaggcac gcgtctggaa tgaatccgtc gaaatccgcc tcagaagcgg
     2341 ctc
//
LOCUS       DATCXU010000154         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1118529,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000154 DATCXU010000000
VERSION     DATCXU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118529"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /gene="glcE"
                     /locus_tag="VIR04_03415"
     CDS             complement(<1..688)
                     /gene="glcE"
                     /locus_tag="VIR04_03415"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY9459829.1"
                     /translation="MEFALSELSNQVMAARAALRPVFIRGGGTKSFYGEIDGLEDISS
                     YSVLDMSAYQGIVNYQPSELVITAKAGTLLSEIEQTLNDQNQMLAFEPPRFGPRSTIG
                     GCVSAGLSGPRRMAAGSLRDFILGTRLLDSTGAVLQFGGEVMKNVAGYDVSRLLAGSM
                     GIFGALLEVSIKVAPRPFLEETRVLDVDQAGALAFFGQWRGQPLPISASAWHADSDHP
                     AGRLWVRLSGS"
     gene            complement(698..2191)
                     /locus_tag="VIR04_03420"
     CDS             complement(698..2191)
                     /locus_tag="VIR04_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY9459830.1"
                     /translation="MKDSVIPTAALPGPDFEGLIARLQQKIPEQCILTKTEDTRPYEC
                     DGLPLYKALPPVVVLPETEAQVIEILRACKAFSIPIVARGAGTGLSGGATPHTRGIVL
                     GMAKFNKIKNIDVQTATAVVQPGVRNLAISEAAHKYGLYYAPDPSSQIACSIGGNVSE
                     NSGGVHCLKYGLTVHNVLRVRMVTIDGEVVELGSEAPDSAGLDLLAVFIGSEGMLGVV
                     TEVTVKLIPMPACARVIMASFSSVEAAGQAVTSVIAAGIIPAGLEMMDQRAVRMVEPF
                     VKAGYDLDAAAILLCESDGTVDEVDDEIKRMEAVFQSAGATRLQVSGSEQERLRFWAG
                     RKNAFPAAGRVSPDYYCMDGTIPRRHLARVLGAMEQMEDKYKLRCANVFHAGDGNLHP
                     LILFDANRPGDVERAEDFGAAILELCIEVGGTVTGEHGVGIEKINQMCVQFKREELDA
                     FSAVKRAFDPYGLLNPEKVIPTLVRCAEYGKMHVHGGDIRFPELPRF"
     gene            complement(2204..>3075)
                     /locus_tag="VIR04_03425"
     CDS             complement(2204..>3075)
                     /locus_tag="VIR04_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY9459831.1"
                     /translation="DGRIINTANRAKKSSAGYDLTRLFVGSEGTLGIITEVTVRLYPL
                     PEAISAAICNFETLDSAVQSVIEIIQMGVPVARVEFMDTAAVKATNAYSKLTLKESPL
                     LLFEFHGSPASVKEQSATVQDITHGNGGTDFQWAEHPEDRSRLWAARHDAYFAGLHMR
                     PGSRSSTTDVCVPISQLARCVGETVKDLDQASFPYTIVGHVGDGNFHVMMLLDPANPA
                     EWQESERLNQRLVDRAIAMDGTCTGEHGIGLHKMEFMLAEHGADALDLMRSLKNAFDP
                     NNVLNPGKMIPLA"
BASE COUNT          705 a          946 c          793 g          631 t
ORIGIN      
        1 cgctgcccga tagccgcacc caaagccggc ctgccggatg atcggagtct gcatgccagg
       61 ccgacgctga aatcggcaaa ggctggccgc gccattggcc aaaaaatgcc agcgcacccg
      121 cctggtcgac gtcaagcacc cgagtctcct cgagaaaggg ccggggcgcc accttgatgg
      181 acacttcaag caacgcgccg aaaataccca ttgatcccgc cagcaagcgt gacacgtcgt
      241 aaccagcaac gtttttcata acctcgccgc caaactgaag cacggcgccg gtcgaatcaa
      301 gcaaacgagt gccaagaata aaatcccgca gactgcccgc ggccatacgc ctgggcccgg
      361 acaagccggc cgaaacgcac ccgccgatcg tcgaacgcgg cccgaatcgg ggcggttcaa
      421 acgccagcat ctggttttga tcgttcaatg tctgttcaat ttccgacaaa agcgttcccg
      481 ctttagccgt gatcaccagc tcggatggct gataattaac aataccctgg tacgcggaca
      541 tatcgagcac ggaataactg ctgatgtctt ccagaccgtc gatttcgcca tagaaacttt
      601 tagtcccgcc gccccgaata aatacaggac gcaaagcagc gcgcgcggcc atgacctggt
      661 tgcttagctc ggataaagca aactccatct tctaccctca aaaacgtggc aattcgggaa
      721 agcggatgtc cccgccatga acatgcatct tgccgtactc ggcgcaacgc accagcgttg
      781 gaatgacctt ttccggattc agcaggccat agggatcgaa agcccttttc accgcggaga
      841 acgcgtcaag ctcctcgcgc ttgaactgca cgcacatctg gtttattttt tcgatcccca
      901 caccatgttc gcccgtgacc gtgccgccca cttcaatgca tagttcgagt atggcggcgc
      961 caaaatcctc ggcgcgttcc acatcgcccg gccgatttgc atcgaacagg atcaatgggt
     1021 gcagattgcc gtcgcccgca tgaaaaacgt tagcgcaacg caacttgtat ttatcttcca
     1081 tctgctccat tgcgccaaga acacgggcaa ggtgacgacg cggaattgtg ccgtccatgc
     1141 aatagtaatc aggcgatacc cggccagccg cgggaaacgc atttttccgt cctgcccaga
     1201 aacgaagccg ctcttgctct gaaccggaca cctgcaaacg agtcgcgccc gccgactgaa
     1261 atacggcttc catgcgcttg atttcgtcat cgacctcgtc aaccgtgcca tctgattcgc
     1321 agagcaaaat cgcggcggca tccaggtcgt atcccgcctt tacaaacggt tcgaccatgc
     1381 gcacggcgcg ctgatccatc atctcaaggc ccgctggaat aatgcccgcc gcgattacgc
     1441 tggtgacagc ctgcccggcc gcctcgacgc tggaaaagct ggccataatc acgcgggcgc
     1501 acgcgggcat gggaataagt ttgacggtga cctccgtcac cacgcccagc attccttccg
     1561 aaccgataaa caccgccagc aagtccagac cggcgctatc gggcgcctcg gagcccagct
     1621 cgaccacctc tccgtcaatg gtcaccatgc gcacgcgcaa aacgttatga acagtcagac
     1681 cgtatttcag gcaatgcacg ccgcctgaat tctcggagac gttaccgcca atggaacagg
     1741 caatctggct ggacgggtca ggtgcgtaat acagaccgta tttatgagct gcttcggaaa
     1801 tcgccagatt gcgcacgccc ggttgcacca ccgccgtcgc ggtttgaacg tcgatgttct
     1861 ttattttgtt gaacttggcc atacccagca caataccccg ggtatgcggc gttgcgccgc
     1921 cagaaaggcc ggttccggcg ccgcgcgcga cgatgggaat gctgaacgcc ttgcaagctc
     1981 gcagtatttc aataacctga gcctcagttt cgggtagcac caccactggc ggcaaagctt
     2041 tatacagcgg caggccatcg cattcgtagg gccgcgtgtc ctcggttttg gtcagaatgc
     2101 actgttcggg aatcttctgt tgaagacgtg cgatcaggcc ctcgaaatca ggtccaggca
     2161 aggcggccgt tggtataaca ctgtctttca tctaaaaccc tttttacgcc aacggaatca
     2221 tttttcctgg attcaaaaca ttattcggat caaatgcatt cttcagactg cgcatcagat
     2281 cgagcgcgtc agcgccgtgt tcagccagca taaattccat tttatgcaaa ccgataccat
     2341 gttcgccggt gcatgtgccg tccatcgcga tagcgcgatc aaccaaacgc tgattcagac
     2401 gctcggactc ttgccattcc gctggattgg cggggtcgag caacatcatc acatgaaaat
     2461 tgccatcgcc cacatggccc acgatcgtgt agggaaaact ggcctggtcc aggtctttga
     2521 ctgtttcacc gacacaacgc gccaattgcg aaatggggac gcacacatcg gtcgtgcttg
     2581 aacgcgagcc cgggcgcata tgcaaaccgg caaagtatgc gtcgtggcgc gccgcccata
     2641 aacgactgcg gtcttcggga tgctcggccc actgaaaatc ggtgccgcca ttaccgtggg
     2701 tgatgtcctg cactgtggcc gattgttctt taacactggc cgggctgcca tgaaattcaa
     2761 atagaagcag cggcgattcc ttcagcgtga gcttgctgta tgcgttagtc gccttgaccg
     2821 cagcggtatc catgaattcc acgcgtgcaa caggcacccc catttgtatg atctcgatca
     2881 cgctttgaac cgccgaatca agcgtctcga aattgcaaat agccgcggat atggcttcgg
     2941 gcaatgggta cagtcgcacg gttacctcgg tgataatgcc gagcgtgcct tcactgccca
     3001 caaataaccg cgtcaagtcg taacccgccg acgacttctt ggcgcgatta gccgtgttga
     3061 taatgcggcc atcgg
//
LOCUS       DATCXU010000155         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_687170,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000155 DATCXU010000000
VERSION     DATCXU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687170"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VIR04_03430"
     CDS             complement(<1..845)
                     /locus_tag="VIR04_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459832.1"
                     /translation="MDLLLQVLVNGILQGGLYAIMALGLALVWGVLNIVNLAHGAFIM
                     VGAYISWHLFTYLHLDPFIGLPITAIVMFGLGYAIQRGILNLIARAPMFNTLLITFGF
                     EVILTYLVQLAFSADFKTINPSYAGSSFTLGVVTVPLVRFAAFGAALVLTLAMWIFLL
                     HTRLGRAIRATAQNLVAARLYGVEPRHLYAVTFGLGLALAGAAGGLYGTVSQINPYIG
                     ASLTVKSFAIGIVGGLDSPLGAIVGGLIIGVVEALTALYIGPTYTDVASFGILILVLI
                     VRPSG"
     gene            complement(878..2095)
                     /locus_tag="VIR04_03435"
     CDS             complement(878..2095)
                     /locus_tag="VIR04_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9459833.1"
                     /translation="MFFKSLRHVPFLLTTLALSLGLSNAQAAPAEIVLGTSVQLTGAD
                     ANTGRYYQDAYKFAIDKINEAGGVKVDGKSYKLALKLYDNQSDVNLSVRQYTQLVTQD
                     KVNFLLGGFASNFSVADSAVSEKYKIPMVQGGGASDQIFSRGFKYIFGTLPAASNYFG
                     STIAMLKKVKPAPESVALLYADDSFDVSVAKGTRPLLKAAGLKVVMDEKYSDNASDFN
                     SLLSQVKAKKVDAVLVAGHETGILNFVRQAKSLNVAPKMYSFTVGVPSQDFRKALGND
                     ADYAFGMTAWIPNASLKDKWFGDAVQFETAYKAKYGYEPDYHAASGASDVETIANAIE
                     TANSLDPKKVRDAISASNFDSLYGKIAFSPSGQISLDQTVIQVQKSVLKPVFNGKDFI
                     NPVQYPMPSWSGR"
     gene            2289..2663
                     /locus_tag="VIR04_03440"
     CDS             2289..2663
                     /locus_tag="VIR04_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEY9459834.1"
                     /translation="MSCVVRAVVVGADRLGNIPELLKEHNIAIAHHISGRDPSHQKKV
                     CQLPSGAELLILLTDFLGHNVMKAFRSAARRSGIRVLACRRSVCSMKQALDHCGYCEL
                     CSAETVGSAQPAARFHRQSCAN"
     gene            complement(2969..3375)
                     /locus_tag="VIR04_03445"
                     /pseudo
     CDS             complement(2969..3375)
                     /locus_tag="VIR04_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT          733 a          868 c          978 g          796 t
ORIGIN      
        1 aagccgctag gtcgaacaat gagcacaagt atcaaaatcc cgaaactcgc gacgtccgtg
       61 taggtgggcc cgatataaag cgcggtcagg gcctcgacga cgcctatgat cagaccgcca
      121 acgatggctc ccagcggcga atccaggccg ccgacgatgc caatggcaaa cgatttgact
      181 gtcagcgatg cgccaatgta gggattgatt tgcgacaccg tgccgtacag tccgccagcc
      241 gcgcccgcca gggccagccc caggccgaag gtaacggcgt acaaatggcg cggctccacg
      301 ccatacagcc gcgcggccac cagattttgc gcggtggcgc ggattgcccg ccccaaccgt
      361 gtgtggagca gaaaaatcca cattgcaagg gtcagaacca gcgctgctcc aaatgcggca
      421 aaacgcacca gaggcaccgt aactacgccc aaggtgaagc tgcttcccgc ataggaaggg
      481 ttgatcgtct taaagtcggc cgaaaacgcc agttgcacca gatatgtcag gatcacttca
      541 aatccaaacg taatcagcaa ggtgttgaac atgggcgcgc gtgcgatcaa gttcaagatg
      601 cctcgctgga tggcgtatcc aagaccgaac atcacgatag cggttattgg cagacctatg
      661 aaagggtcca gatgcaaata ggtgaataaa tgccaggaaa tataggcgcc caccataatg
      721 aaggcgccgt gggcaaggtt aacaatgttc aacacacccc ataccaaagc aaggccgagc
      781 gccataatgg cgtacagccc gccctggagg attccgttga cgaggacttg taacagtaaa
      841 tccacgctaa ctcctcttag tcatactggg ttgtggatta acggcctgac caggaaggca
      901 tgggatattg aaccggattg atgaaatctt tgccattgaa gacaggttta agtacgctct
      961 tttgcacctg gatcacggtt tggtcgaggc tgatctgtcc cgatgggctg aatgcgatct
     1021 tgccgtacag gctgtcgaag ttgctggcgg aaatcgcgtc gcgcaccttt ttgggatcca
     1081 gcgagttggc tgtttcgatt gcgtttgcga tggtttcaac gtcgctcgcg cccgatgccg
     1141 catggtagtc gggttcgtag ccgtatttgg ctttgtaggc ggtttcaaat tggacggcat
     1201 cgccgaacca cttgtctttc agcgacgcgt ttggaatcca ggcggtcatg ccgaaagcgt
     1261 aatcggcgtc gttgcccaat gctttgcgga agtcctggct gggaacgccc accgtgaacg
     1321 aatacatttt tggagcgaca ttcagactct tggcctgacg gacaaaattc aaaattccgg
     1381 tttcgtgacc agccaccaga accgcgtcaa ccttttttgc tttgacttgg gacagcaggg
     1441 aattgaagtc gctggcgttg tcactgtatt tttcatccat tacgaccttc aggccagccg
     1501 ctttcagtaa aggacgcgtg cctttggcaa ccgaaacatc gaaggaatca tcggcgtaca
     1561 gcagggcaac ggactcgggc gccggcttta cttttttcag catggcgata gtgctgccga
     1621 agtagttgct ggctgcaggc agtgtcccga aaatatattt aaagccgcgc gagaaaatct
     1681 gatccgaggc gccgcctccc tggaccatgg ggattttata tttttccgat acagccgaat
     1741 ccgcgacgct gaaattgctg gcaaagccgc ccagcaggaa attgaccttg tcctgcgtta
     1801 ccagttgcgt atattggcgc acgctcagat tcacatcgga ctggttgtca taaagtttta
     1861 gcgcaagttt gtaggatttt ccgtcaacct tgacaccgcc ggcttcgttg attttatcga
     1921 tggcgaattt atacgcgtct tgataatagc ggccggtatt ggcatcggcg ccggtgagtt
     1981 gcacagatgt ccccaaaacg atttcggcgg gtgcggcttg cgcatttgac agtccgaggg
     2041 acagcgcaag cgtcgtcagc aagaaaggga catgacgtaa agatttgaaa aacattaggt
     2101 gactcctcta tatagattgc gtaatatttt gcatattttt ctttacattt agctgaatga
     2161 cgtaagggtt tcctctgatt tagctaggga aacgggcact caaaccaata ttttttgaca
     2221 atgctaatac gaatgattat tattacgtaa ggtatttatt cgctgaaaat cgttttcatg
     2281 gagtgcttat gtcgtgtgtg gtcagggccg ttgtggtcgg ggcggatcga ctgggaaata
     2341 ttcccgaact gttaaaagaa cacaatattg ccatagcgca ccatatcagc gggcgcgatc
     2401 cgtcgcatca gaagaaggtg tgccaattgc cgtcgggcgc cgaattgttg attttattga
     2461 cggacttttt ggggcataac gtgatgaagg ccttcaggtc tgccgcgcgg cgctccggaa
     2521 ttcgagttct ggcgtgccgg cgttcggtct gcagtatgaa gcaagccttg gatcattgcg
     2581 gctattgcga gctttgctca gccgaaacgg tgggttcagc gcaaccggcg gcgaggttcc
     2641 accgccagtc ttgtgcgaat taatcgcgtc tgctgatcgt ttttactgtc tttttgtggc
     2701 cggtgttatg cgcactggcc acgtgtgtgg cgccacgcgc gtgcgatttg gctgcactgc
     2761 gcgcggatgc tgcgtggtct ttggttgcgc cccgggtgcg cgccttgacg ctgcgggctg
     2821 cgacgtgcgc cgtggcgcgt gcatgtgtcg ttttggatgc atgcctggta ctgcgcgtgc
     2881 gcgttgtgtt ggccgtgtcg gtcagtgccg gagcctcggc cgcatcggct gcatcgatta
     2941 atgcgggcat gtcggtcgta ttggccgcaa ggcggcgggc gccgttgtac cgttttttcc
     3001 agtaggcggc gtccatgctg tcaacgcgca ccaccgcgcc gcttcttgga gcgtgtacga
     3061 attcgtggtt gcccaggtag atcccaacat gcgagttgcg cgatctgcgt gtattgaaga
     3121 agacgaggtc tccttttttg agctcatcgc gatcgacggt gaagccttcg cgagccagtt
     3181 cggctgaacg gcgcggcaac ttgaggccga gcgatttttc cgcggcataa gcgacaaggc
     3241 cgctgcaatc gaacccttct ttaggcgtgt cgccaccgaa ccggtacttg attcccagga
     3301 agttaagcgc agtttgcgca agcggcttgt ccgagcgcgg cgcgcgcgac atgcgcgaca
     3361 tgcgcgaact gccgc
//
LOCUS       DATCXU010000156         8057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_411034,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000156 DATCXU010000000
VERSION     DATCXU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411034"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..690)
                     /locus_tag="VIR04_03450"
     CDS             complement(46..690)
                     /locus_tag="VIR04_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY9459835.1"
                     /translation="MTTNNGSGKTSAQAKEQATPPVANELGKRLRRQRLAAGFTLEQL
                     ATQAGFDKGYLSRIENGKKVPPIATLSRLAGALGIEAATLLSAPGTTAPWRGVSVVRH
                     DEKRPTVLGGSAFGYDYFALSDAATSRALQPFLFSFPAEIDKFVFFQHDGEELLYVLT
                     GRVEWQVGMQKYILEAGDAIHFDSRMPHRGHSLSGAATALVIMYSPAGLHENLA"
     gene            861..1901
                     /locus_tag="VIR04_03455"
     CDS             861..1901
                     /locus_tag="VIR04_03455"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459836.1"
                     /translation="MQNLKGGFNAIKSATRKTLAAASAVLLLTAATAGAQAQTLTKVR
                     YQETVRSLQFTTAYVAMARGYFKDAGLDLDMKTAQGTDKAMAALLSGSADIALLGPEA
                     VIYVANSESPAKPKIISGLVATDGFLLVERNTDKSPQKFDWNDLKGKTVMAYRPGSTP
                     DVYLGSLLRKHHLAVGTDLKIVNNIGPAARMGAWLTGKADYAIFLEPEASTIERQGKG
                     KIVTSIGNEVGLVDYTVFAAMPDYIASHPKVIKGWVTAIARAMKDVATDQPAVIAKDL
                     VSYFPGLSQAELVQSIERYRKYGVWKKTPQVAESAINTLQDMLIASKTLKGTEHVPYA
                     RVVAPELAEYAK"
     gene            1898..2722
                     /locus_tag="VIR04_03460"
     CDS             1898..2722
                     /locus_tag="VIR04_03460"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459837.1"
                     /translation="MTASDSINAASPESANNRIELQDVSLSYFTPEGETLALDQISLT
                     IDPGEFVSIVGPSGCGKSTLLSIIAGILPASSGHVILGGQTIKGPSPRIGYMLQQDYL
                     FEWRNILDNATLGAEIQKLDRRRAQERATRLLQKYGMGEFLHHYPRELSGGMRQRVAL
                     ARTLVTEPDVVLLDEPFSALDSQTRLALADEVVEILRNEGKSVVLVTHDLGEAITMTN
                     RVVVLSRRPGRVKSEHAITFASHPGSHPAPFKARTLPEYNKYFTTLWGELDVHVER"
     gene            2749..3591
                     /locus_tag="VIR04_03465"
     CDS             2749..3591
                     /locus_tag="VIR04_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459838.1"
                     /translation="MTSARSSKDLAKTSSQAAPSTAYKEYLHRRKIRRVLILACQFLL
                     FVLFFAVWEILARTHVVNPLLTSYPSALWPTFLDLLHNNNLLTHTWSTLRATLVGFSI
                     SMALGVLIAAMLWVSDFLYKVLDPFMVIANAMPKIALVPIFYIWLGADYSIYGMAVAI
                     AVFVTIIVMYGAFRSIDENRIKLARTFGASRIQVLHLVVLPGSVPVLFSAIKMNIGLS
                     LIGVIVGEFQSASSGLGFLIINGSQIFKLNIVMTAVVVLAAMSGVMYLIVARLEAAVA
                     QRYR"
     gene            complement(3588..4751)
                     /locus_tag="VIR04_03470"
     CDS             complement(3588..4751)
                     /locus_tag="VIR04_03470"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEY9459839.1"
                     /translation="MTTSMISSKTASIRTSAAAPNSFFSSSYGIARARFLHAAAQAGA
                     VVHQYVHPTVRAPDGGTLSCDVAVLGPKDAKKAFVAVAGTHGPEGYAGSAAHLMLLQS
                     GMARRLAPDCRIVLIHAINPYGFAYGSRTTENNVDLNRNFIDHGKPHPYNALYPSLHS
                     IVCSDKYSKALHGAQVRAMLAWTKEFGINARNQALIAGQYSHPSGRTYGGRNREWSNL
                     VLERIAVEHLHGVERIAFIDWHTGLGKYGEEFFLCFNEPGSETYEQCVKWWGADRIQS
                     SGGFEGAERPRYQGLLFSGLSDFVRPAKFAGAVVEFGILSNDEQDAVFMLEYWLRFGD
                     HSGVSVSAMKKVRKELAEAFTPSAPEWRSSVSKRSSKILQEALSGLSGWQAAG"
     gene            complement(4761..5609)
                     /locus_tag="VIR04_03475"
     CDS             complement(4761..5609)
                     /locus_tag="VIR04_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY9459840.1"
                     /translation="MRTFSDGIDHVVIVVGDLDSAAQDYRKLGFLPTPKGRHTMGSEN
                     HCVMFASDYFELLGFPPGARAAQALLDFARRGSGLAAVVLRTSDAQAMRDELNADGIS
                     ASDIRDFSRPVDLDGEVVQARFRTVDMPASLTPGLHVFACQHYTREATWRRGYCEHPN
                     GVTGLKDVILISNRPERDLAQYARLLDRPAIDMGGFWSLSFDSGAAITAVSKDRADHI
                     VQGLAGMVTRPTSCAAALRFFVDDLGTLADLFKRNQVAFKRVPGQQLLVGPQSAHGVA
                     LVFCAN"
     gene            5766..5960
                     /locus_tag="VIR04_03480"
     CDS             5766..5960
                     /locus_tag="VIR04_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEY9459841.1"
                     /translation="MTRRYIDCRNFPSEMNCSVAISADSEKELMEAAVQHAMAVHGHK
                     DSPELRDQVKKSIREGSPPN"
     gene            complement(5976..6332)
                     /locus_tag="VIR04_03485"
     CDS             complement(5976..6332)
                     /locus_tag="VIR04_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY9459842.1"
                     /translation="MSEPIQRHHTSARMSKIVRHGGLVFLCGQTATRGKSVTIADQTR
                     EVLGKIDALLTQAGTDRERILSATLYIKSMNDFAAMNVVWEDWIPPGAAPARTTVEAS
                     LAAPELLIEITVIAAA"
     gene            complement(6356..7393)
                     /locus_tag="VIR04_03490"
     CDS             complement(6356..7393)
                     /locus_tag="VIR04_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY9459843.1"
                     /translation="MIEGRKNVVRLSRWTNSIFDETLALESDISLTVCSVAGTETKTR
                     SALEEAHVYHVPAARDEVPQRWLVTPELLKNCPSLVCVSSVGSGYDTIDVDACTRAGV
                     AVVNQAGGNANSVAEHTLGLMLSVSRRICESNRRLRHEREFSREDVMGNEIGGKVLGI
                     VGIGHVGTRVAALARAFNMTVLAYDPYLTNAQILERGATPVAFNDLLERSDIVSLHCP
                     RTDESMGLIGARELGQMKEGSLFITTARGGIHDEAALLDALQSGHIGGAGLDVWDHEP
                     PPRNHPLLQLDSVVATFHTAGVTHEARYNVAAMAAEQIVGILRGARPPRLINPEVWPA
                     YLARFSAVLFT"
     gene            complement(7408..>8057)
                     /locus_tag="VIR04_03495"
     CDS             complement(7408..>8057)
                     /locus_tag="VIR04_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459844.1"
                     /translation="RPPGLTNRGQGRSALSAAYLIAIVRQKWMRRVIWSGLALAGSQC
                     GIVVYLMLFLRDRLGIPLYQGAFFLFLVQLFGGAGRILLSTWSDRCGKAGKRFFPVSV
                     SMISTVLGLLALIHLPAEAPVFMLVGVVGWLGFFALGWYGPWVTYVADSSPQDSVGLA
                     LGAAMTVTQIAIVSAPPLLGMVHDFTQNYDFLWYSLIAWITVAGWVIRPGQAISH"
BASE COUNT         1892 a         2382 c         2157 g         1626 t
ORIGIN      
        1 tgacaggcga agcgctggaa taaaaaggcg cgccttccgg cgaggtcatg caaggttttc
       61 gtgcagcccc gccggcgaat acataatcac caacgccgtc gcggcgcccg acaagctatg
      121 tccgcgatgg ggcatgcgcg aatcgaagtg gatggcgtcg ccggcttcga gtatgtactt
      181 ctgcatgccc acctgccact cgaccctgcc tgtcaacacg taaagcagtt cttctccgtc
      241 gtgctgaaaa aagacaaatt tatcgatttc ggccgggaaa gaaaatagaa aaggctgaag
      301 cgcccgggat gtcgccgcgt cgcttaatgc aaaataatca tacccaaacg cactgccgcc
      361 cagcacggtc ggacgctttt catcgtgccg gacaacgctg accccgcgcc agggcgccgt
      421 tgtccccggc gctgacaaca aggtggcggc ctcgattcca agcgcccccg caagtcgcga
      481 cagcgttgca attggcggaa ctttcttgcc gttttcgatt cgggacaaat agcccttatc
      541 aaaccctgct tgcgtcgcca actgttcgag cgtaaagcca gcggccagtc tttgtcgacg
      601 taatcgtttg ccaagctcgt tcgccacagg gggagttgct tgctcttttg cttgcgcaga
      661 agtcttgccg gagccattgt tcgtcgtcat agccgtattt tatcagggcg gcaggttcca
      721 cggcgacgcg cgctccggcc caatataacc ccggttcaat ataagcttat acacggactc
      781 gcgttgttac aggagtgaaa cggcactatc atttaagtct tacacagaca caacgtttac
      841 acaaacacgg agaaatcacg gtgcagaatc tgaagggcgg ctttaacgcc ataaaaagcg
      901 ctacgcgcaa aaccttggcg gcggcaagcg ccgtgctgct gttgaccgct gctaccgccg
      961 gcgcccaagc ccagacactg accaaagtac gataccagga aacggtgcgc tcgctgcagt
     1021 tcacgaccgc gtatgtcgcg atggcgcgag gctacttcaa ggacgccgga ctggatcttg
     1081 acatgaagac ggcgcaagga acggacaagg cgatggcggc actgttatcg ggcagcgccg
     1141 acattgcgct gcttggccca gaggccgtga tctacgttgc caatagcgaa tcaccggcca
     1201 aaccaaaaat catctccggg ctggtcgcca ccgatggctt cctgcttgtc gaacgcaata
     1261 ccgacaagag cccgcaaaaa tttgactgga acgacctgaa aggcaagacg gtcatggcgt
     1321 accgtcctgg ctcaactccg gatgtttacc taggttcgct gctgcgcaag catcacctcg
     1381 ccgtcggcac cgatcttaaa atagtcaaca acatcggccc cgccgcgcgg atgggagcct
     1441 ggctgacggg caaagccgat tacgcaattt ttctggagcc ggaagccagc acgattgaac
     1501 gccagggcaa gggcaagatc gtcacatcaa taggaaatga agtcggcctc gtggactaca
     1561 cggtatttgc cgcaatgccc gactacatcg cctcgcatcc gaaagtgata aagggctggg
     1621 tgacagcaat tgcacgggcc atgaaagatg tcgccaccga tcagcccgcc gtcatcgcga
     1681 aggacctggt cagctatttc ccgggcttgt cacaggcgga actggttcag tcgatagagc
     1741 gctaccgcaa atatggcgta tggaagaaaa caccgcaagt tgcggaatcc gccataaaca
     1801 cgctgcagga catgcttatt gccagcaaga cactaaaggg aactgaacat gtcccgtatg
     1861 cgcgcgtggt ggctccggag ctggcagagt atgccaagtg actgcgtcgg actccataaa
     1921 tgcggcaagc cccgaaagcg ccaataaccg aatcgaactc caggacgttt cgctgagcta
     1981 tttcacgccg gaaggcgaaa cgctggcgct cgaccagatt tcgctaacta ttgacccagg
     2041 cgaatttgtc agtattgtcg gcccctcggg ttgcggtaaa agcacgttgc tttccatcat
     2101 tgcaggaatc ttgcccgctt cttcggggca cgtcatcctt ggaggtcaaa ccatcaaggg
     2161 gccctcgccc agaatcggtt atatgcttca acaagattat ctgtttgagt ggcgcaatat
     2221 tctggataac gccacccttg gcgccgaaat acaaaaactg gaccgtcggc gcgcgcaaga
     2281 gcgcgccacg cgccttctgc agaaatacgg catgggcgaa ttcctgcacc actacccgcg
     2341 ggaattatcg ggcggaatga ggcagcgcgt agcgctggcg cgcacgctgg ttacagagcc
     2401 cgacgtcgtt ttactggacg aaccgttttc ggctctggat tcgcaaaccc ggctggcgct
     2461 ggccgacgaa gtcgtcgaaa ttcttcgcaa tgaaggcaaa tcagttgtac tggtgaccca
     2521 cgaccttggg gaggccatca ccatgaccaa tcgggtagtg gtgctgtcca ggcggccggg
     2581 ccgtgtcaag tccgaacacg ccatcacatt cgccagccac cctggctccc acccggcccc
     2641 attcaaagcc cgcacgctgc ctgaatacaa taagtacttc accaccctat ggggcgaact
     2701 cgacgttcat gttgaacgtt gaactgcctt tggaagccgc tatcactcat gacatccgca
     2761 cgttcgtcca aagacttggc aaagacttcg tcgcaggcgg cgcccagcac ggcctataaa
     2821 gaatatttgc atcggcgaaa gattcgccgt gttctgatac tggcctgcca atttctactt
     2881 ttcgtccttt ttttcgccgt gtgggaaata ctggcccgaa cgcatgtcgt gaacccgctg
     2941 ctaaccagtt atccgagcgc gctctggccg acattcctgg atctgcttca caataacaat
     3001 ctgctgaccc atacgtggag cacactgcga gcgacgttgg tcgggttttc catcagcatg
     3061 gcgctgggcg tgctaattgc ggcaatgctt tgggtttccg atttcctgta caaggtgctg
     3121 gatcccttta tggtcatcgc gaatgccatg ccaaaaattg cgctggtccc gattttttat
     3181 atttggctgg gggcggatta ttccatctac ggcatggcgg tagccattgc cgtgttcgtc
     3241 accatcatcg tgatgtacgg cgcttttcgc agcatcgacg aaaacaggat caaactggcg
     3301 cgcacctttg gcgccagccg cattcaagta ttgcatcttg tggttttacc gggcagcgtg
     3361 cctgtactgt tttccgccat taagatgaac attggcctat cgttgatcgg cgttatcgta
     3421 ggcgagttcc aatccgcaag ctcgggtctt ggtttcctta tcatcaacgg cagccaaata
     3481 ttcaagctga atattgtgat gaccgctgtc gtcgtgctgg ccgcaatgtc gggtgtaatg
     3541 tacctgatcg tggcgcggct ggaagcggcg gtcgcacaac gctaccgcta accagccgcc
     3601 tgccagccac tcaatcccga caacgcttcc tgcaatattt tcgatgaacg cttagagaca
     3661 gaggatcgcc attcgggcgc agacggcgta aatgcttccg cgagctcctt cctgactttt
     3721 ttcatggctg acacgctaac gccagagtga tccccaaatc gcagccagta ttcaagcatg
     3781 aacacggcgt cttgctcgtc gttgctcagg atgccgaatt cgacgacggc gccggcaaat
     3841 ttcgccggtc ttacaaagtc cgataaaccg ctaaacagca gcccttgata gcgtgggcgc
     3901 tcggcgcctt cgaacccgcc cgatgactga attcgatcgg cgccccacca tttgacgcat
     3961 tgttcatagg tttcggaccc cggttcgtta aaacacagga aaaattcttc gccgtatttg
     4021 cccaagccgg tatgccagtc gataaacgca atacgctcca cgccgtgcaa gtgctcgacg
     4081 gcgatccgct ccagaacaag gttggaccac tcgcgattgc gcccgccata tgtccgtcca
     4141 ctcgggtgcg aatactgtcc cgcgataagc gcctgattgc gcgcattaat gccgaattct
     4201 ttagtccatg ccagcatggc cctgacctga gcgccgtgca gcgccttgct atatttatct
     4261 gaacaaacaa tcgaatgaag ggaaggataa agcgcgttgt acggatgggg ttttccgtgg
     4321 tcgatgaaat tgcgattcaa atcgacatta ttttccgtgg tgcgcgagcc ataagcaaaa
     4381 ccataggggt taatggcgtg aatcaacact atgcggcaat caggcgccaa acgcctggcc
     4441 atacccgact ggagcagcat taaatgggcc gccgagcccg cgtagccctc gggtccatga
     4501 gtccccgcga cagcgacaaa tgctttcttt gcatctttcg gccccaacac tgccacgtcg
     4561 cagcttagcg tgccgccatc gggggcccgc accgtcggat gcacatattg atgaaccacc
     4621 gcgccggcct gcgccgcagc atgcaaaaac cgtgcgcgtg caatgccgta gctggaagaa
     4681 aaaaaggagt tcggcgcggc ggcagaagtg cggatcgaag ccgtcttcga tgaaatcatt
     4741 gaagtggtca ttatgttgaa ttaattagcg caaaatacca gagccacgcc gtgcgccgac
     4801 tgaggcccaa ccagcaactg ttgccccgga acccgcttga aagcgacctg attgcgcttg
     4861 aacaaatccg ccagcgtgcc cagatcatcc acgaagaaac gcaaggccgc ggcgcaagac
     4921 gtgggtcgag tcaccatacc tgccaggcct tgaacaatat ggtctgccct gtctttcgac
     4981 accgccgtga ttgccgcgcc cgaatcgaac gacagggacc agaaaccgcc catatcaatg
     5041 gccggcctat ccaaaagccg cgcgtattgc gccagatcgc gctcgggtcg gttgctgatc
     5101 aatatcacat ccttcaagcc agtcacccca ttcggatgct cgcaataccc cctgcgccat
     5161 gtggcctccc gagtgtagtg ctggcaagcg aacacatgca ggccgggcgt gagcgaagcc
     5221 ggcatgtcca ccgtgcgaaa tcgggcctga accacttccc catccagatc gacaggccgt
     5281 gaaaaatctc tgatatcgct tgcagatatg ccatcggcat tcagttcatc acgcatggct
     5341 tgagcatcgg aggtacgcaa gacgaccgcg gccaggccgg aacctcggcg cgcaaaatcc
     5401 aaaagcgcct gcgccgcgcg cgcccccggg ggaaagccta gaagctcgaa gtagtctgag
     5461 gcgaacatta cacagtggtt ttccgagccc atggtgtggc ggcctttcgg cgtcggcagg
     5521 aaacccagtt tccggtagtc ttgcgcagca ctgtccagat cgccgaccac gatcacaaca
     5581 tgatcaatgc cgtcagaaaa cgttctcata tccactcttt cgtaaaaatg acccgatatg
     5641 gttttagcac acgccgaaaa acagcggaaa acgttcgttg caaaagatac aacgtccacg
     5701 taatgggagg atttaaaatc ccgcttgtag ctacgcatca gaaggccaaa ctgacgggag
     5761 gcgctatgac acgcagatat attgattgtc gcaactttcc gagcgagatg aactgctcgg
     5821 tggcgatatc ggcagacagc gaaaaagagt taatggaggc tgcggtgcaa cacgccatgg
     5881 ccgtccacgg tcacaaagac tcacccgagc ttcgagatca ggtgaaaaag tctatacgag
     5941 agggcagccc tccaaactag ttttccaaaa agctttcagg cggccgcaat gactgtaatt
     6001 tcaatcagca gttcaggcgc cgccaggctt gcctcgacgg tcgtgcgcgc cggtgctgcg
     6061 cccggcggga tccagtcttc ccaaacgaca ttcatggcgg cgaaatcgtt catgcttttt
     6121 atgtatagcg tggccgaaag tatacgctcg cggtcggtgc ccgcttgcgt tagcagcgcg
     6181 tctatcttgc cgagcacttc cctggtctga tcggcaatgg tcacgctttt gccgcgcgta
     6241 gcggtttgac cacatagaaa aaccaggccg ccgtggcgca caattttgct catgcgggcg
     6301 gacgtatggt gacgttgaat gggttcgctc atggtgtttg tctcctgtag tcttgtcatg
     6361 tgaacaatac cgcactaaag cgcgcaaggt aagccggcca tacctcgggg ttgattagcc
     6421 gcggcggccg tgcaccccgc agaatgccca caatctgttc ggcggccatc gcggctacgt
     6481 tgtagcgcgc ctcatgcgtc actcccgccg tatgaaacgt cgcgaccaca ctgtcgagtt
     6541 gaagcagcgg gtgattccgg ggcggcggct cgtgatccca cacgtcaagc ccggcgccgc
     6601 caatatgacc cgattgcagc gcgtcgagca acgcggcctc gtcatggatg ccgccacggg
     6661 cggtggtgat aaataaagag ccctctttca tttggcccag ttcgcgcgcg ccgatcaagc
     6721 ccatggattc gtccgtgcgc ggacaatgca gggaaacaat atcggatcgt tccagtaaat
     6781 cgttgaacgc cacaggtgtc gcgccgcgct ccagaatctg agcattagtc aaataggggt
     6841 cgtacgccag caccgtcata ttaaaggcgc gggcgagcgc cgccacccgt gtgccgacat
     6901 ggccgatccc gactatcccc aacaccttgc cgccaatttc attccccatg acgtcttcgc
     6961 gagagaattc ccgttcatgc ctgagccgcc gattcgattc gcaaatacgt ctggacaccg
     7021 acagcatcaa accgagggtg tgctcggcaa ctgaattagc attgccgcct gcctgattga
     7081 cgacagcaac cccagcccgc gtacaggcgt cgacatcaat ggtgtcgtat cccgaaccca
     7141 cggaagaaac acataccaac gatgggcagt ttttcaatag ctcgggcgta accagccagc
     7201 gttgcggcac ctcgtcccgg gcggcgggca catgataaac atgcgcttct tcaagcgccg
     7261 accgtgtttt cgtttctgtg ccggcaactg agcaaacagt gaggctaatg tcggattcca
     7321 gggccagcgt ttcgtcaaaa atcgagttcg tccatcggct gaggcgcact acatttttac
     7381 gaccctcaat catgttgatg attcctttta atgagaaata gcttggccag gccgaatcac
     7441 ccaacccgcg accgttatcc acgcaatcag tgaataccat aggaaatcgt aattctgcgt
     7501 gaagtcatga accatgccga gcaagggcgg ggccgacaca atcgcgattt gcgttacagt
     7561 catggcggcg cccagggcca ggcccacact gtcttgcggc gaggaatcgg ccacataagt
     7621 aacccacgga ccataccagc caagcgcaaa aaaaccaagc cagccgacaa cgccaaccag
     7681 cataaacacc ggcgcctccg cggggaggtg aataagcgca agcaaaccga gaacagtgga
     7741 aatcatgctg acggacaccg ggaaaaaacg cttcccggcc ttgccgcatc ggtcactcca
     7801 ggtcgataaa agaatgcgtc cggccccgcc aaaaagctga accaggaaca gaaagaatgc
     7861 accctgataa agcggaatgc ccagcctgtc tcgcagaaac agcatcagat acacaacgat
     7921 cccgcattgc gaacccgcaa gcgccagccc cgaccagata acacgcctca tccatttctg
     7981 ccgcactatc gcgatcagat acgcagccga caaagcggac cttccctgcc ctctgttggt
     8041 caggccaggc ggccgat
//
LOCUS       DATCXU010000157         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_845437,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000157 DATCXU010000000
VERSION     DATCXU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845437"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1377)
                     /locus_tag="VIR04_03500"
     CDS             complement(91..1377)
                     /locus_tag="VIR04_03500"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY9459845.1"
                     /translation="MTEPKSETQQAAASASSDKKSAATDTTTSSSAKSAPPATRSRSV
                     WRVAFALVLLLAVVLGVALWYEHQNVEKSNTIVLAQMRSGVNIAQQAGDRAQKALSMV
                     QAQSTQIAGLQRMLKESQAQQQSLEQSLQSLTDSGSNLVLVNDIDHLVTIANQQLLLS
                     GNVPNAVIALETAQAQLARASRPSLSSLQQTINGDLDRLRAVSTIDVALLSSQLAELE
                     QLIARAPLLVPDAAAPGVTDTANQHSARPPARPSRPAQVDAPWWRNALNAINDWSGTA
                     WSSVRQDLGQFISVRRASDPAALLMSPEQASQLRENLQLRIMTAQLALMTRQPKVWAS
                     EMQALTRALDTRYDARAADTAQALKLARRLADVSIKVELPTVNNSLQAIEALRQAQAK
                     AAEQKASQPFSGAVPENGPSDDSALAGAASTEPRDK"
     gene            complement(1405..2217)
                     /locus_tag="VIR04_03505"
     CDS             complement(1405..2217)
                     /locus_tag="VIR04_03505"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY9459846.1"
                     /translation="MDPLIVLTRPGGKNGPLALRLNDAGLSTLVLPALVVQPLACHRS
                     EFPFPDDYDLIVFVSGNAARFYLDQFAQLGSGRQWPAHTLLATVGRASAQPLYDAGFI
                     APTHILHPPSGGQNQAQDSEALWSLLQPCINGLKRVLIIRGETGREWLAQHLEQAGAA
                     VLRHAIYRREPARWTAEQNQQIKTGFELRCPLVCLLTSVESVDAIYENTRAMGLHLLS
                     GQTRFVVIHERVARRLQSLANALSGKVYQPAVKICPPNDEAIFRTIMSTVSL"
     gene            complement(2243..3178)
                     /gene="hemC"
                     /locus_tag="VIR04_03510"
     CDS             complement(2243..3178)
                     /gene="hemC"
                     /locus_tag="VIR04_03510"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY9459847.1"
                     /translation="MSAPNRLVIATRASRLALWQAEHVRDRLIGLYPGCEVVLLTMTT
                     RGDQILDRTLSKVGGKGLFIKELEKALLDGRADLAVHSLKDVPVDLQPPFALTAVLER
                     DDPRDALVSNEFDSLDQLPAGAIVGTSSLRREAQIKVHYPHLKVLPLRGNLDTRLTKL
                     DRGDYAAIVLAAAGLRRLGLSQRIRGYLTPEQSWPAAGQGALAVEIMASRTDLQQWLA
                     PLGSADTTACATAERAVSRVLGGSCQVPLAAFAEIKGAVLNLRALVAEPDGSLIVRSE
                     VSGPVQNARQLGEQAGQDLLDKGAKAILLKLATAL"
     gene            complement(3185..3310)
                     /locus_tag="VIR04_03515"
     CDS             complement(3185..3310)
                     /locus_tag="VIR04_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459848.1"
                     /translation="MGLFKSSDSKKSESGLSLLQGMFILAILGVIAAVIVNKLAG"
     gene            complement(3297..4028)
                     /locus_tag="VIR04_03520"
     CDS             complement(3297..4028)
                     /locus_tag="VIR04_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY9459849.1"
                     /translation="MLEFIETLHWGALFQIILIDILLGGDNAVVIALACRNLQHKQRI
                     QGILWGTAGAIITRVVLIAFALALLTVPFLKMAGALLLLWIGIKLLVPDDDHHDKVKG
                     GSSVMAAVKTIIVADLVMSLDNIIAIAGAAQNTLPDHQLGYVIFGLLLSVPIIVWGST
                     LVLKLIDRFPVVVTLGAGLLGWIAGGMLVTDIFIVDQLGVVPVPVKIMAEAVGAILVV
                     MFGKWFASRKLAAKEAAKEDLHGIV"
     gene            complement(4117..4815)
                     /locus_tag="VIR04_03525"
     CDS             complement(4117..4815)
                     /locus_tag="VIR04_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY9459850.1"
                     /translation="MDFSSTAFWLALLEIIWVNILLSGDNAVVIALAARSLPTSQQFT
                     AIAAGSGAAVVLRILLTVAAAELLLLPWLKIVGAVLLVYIGVTLLLPEAHDEGEAKRY
                     GSIWGAIRTILVADLIMSLDNVVAVAAAAHGDLFLLIVGLGISIPLVVFGSTLLLKVI
                     DRLPIIVWFGAALLGYIAGEMLVSDPALRGVVDSFSGIMHSSPDQLAMAIGAVGAVVV
                     LVLGKVFIARAQTD"
BASE COUNT         1156 a         1479 c         1316 g          956 t
ORIGIN      
        1 taataagtac gttgccgcta tgttcgcgca agaccagcgc cagcccgaca gccgaaacaa
       61 acgccagcag catccagaac catgtcctca ttacttatcc cgtggttcgg tggaagcggc
      121 gcccgccaac gcactgtcgt ccgaaggccc attttcaggc acggcgcccg aaaaaggctg
      181 agacgctttc tgctcggccg ctttcgcctg agcctgacgc aaagcctcga ttgcctgcaa
      241 actattatta accgtgggca gttccacctt gatggataca tcggccaatc gccgagccaa
      301 ctttagcgct tgcgcggtat cggccgcgcg cgcatcgtaa cgcgtatcca gcgcccgcgt
      361 cagcgcttgc atttcagagg cccaaacctt gggctgccgg gtcatcagcg caagctgcgc
      421 ggtcatgatg cgtagctgaa gattttctcg caactggctt gcctgttcgg gcgacattaa
      481 caatgccgcg ggatcgcttg cgcggcgcac gctaataaac tgacccaggt cctggcgcac
      541 cgacgaccag gctgtccctg accaatcgtt aatcgcgttc agcgcattgc gccaccaggg
      601 cgcatcaact tgggcgggcc gcgaaggacg cgccggcggg cgggcgctgt gctgattggc
      661 tgtatccgta acccccggcg ccgcggcgtc ggggacaagc aacggcgccc gggctataag
      721 ttgctccagc tcggccaatt ggcttgaaag caaggctacg tcaatcgttg aaacagcccg
      781 caaccgatcc aggtcgccgt taatggtttg ctgcaaagac gacaggctgg gccggctggc
      841 gcgagccagt tgcgcttgcg ccgtttcgag cgcgatcacc gcattgggca cattgccgct
      901 aagcaaaagc tgctgatttg caatcgtaac cagatggtcg atatcgttga ccagaacaag
      961 gttggatccg ctatcggtca aactttgaag ggactgttca agactttgtt gctgcgcctg
     1021 ggattctttc agcatgcgct gcaatccggc gatttgcgtc gattgagctt gcaccattga
     1081 cagcgctttt tgcgcccgat cgcctgcctg ttgcgcgata ttcacgccgc tacgcatttg
     1141 agcaagaaca atcgtgttgc ttttttcgac attctgatgc tcgtaccaga gcgccacgcc
     1201 caagaccacg gcgagcaata aaacaagggc aaaagcaacg cgccacaccg aacgcgaccg
     1261 cgtcgcgggc ggcgcgcttt ttgccgacga tgaggtcgtt gtatcggtcg ccgccgactt
     1321 tttatcggac gatgccgatg ctgcagcctg ctgggtctcg gatttcggtt cagtcatggg
     1381 tggattgtaa tccttggcgc ttttttatag ggaaacggtc gacataatag tccggaaaat
     1441 agcctcatcg ttgggcggac atattttcac tgccggctga tacaccttac cagaaagcgc
     1501 attagccagc gactggagac gccgcgccac gcgttcatga atgaccacaa aacgagtctg
     1561 tccggacagc aagtgcaggc ccatggcgcg ggtgttctcg taaatcgcgt ccacactttc
     1621 gacgctggtc aataaacaaa ccagcggaca gcgcagttcg aatcccgtct tgatctgctg
     1681 attttgttcg gccgtccagc gggctggctc gcgtcgatag atggcatggc gcagaaccgc
     1741 cgcgcccgcc tgctccaagt gttgcgcaag ccactcgcgg ccggtttcgc cccggatgat
     1801 caaaacccgc ttcagcccat ttatacaagg ctgcagcaag gaccagagcg cttcagagtc
     1861 ttgcgcctgg ttctggccgc ccgaaggcgg gtgcaaaata tgcgtgggcg cgatgaatcc
     1921 ggcatcgtaa agaggctggg cacttgcccg gcccaccgtc gccagcaggg tatgcgcggg
     1981 ccattgccgg ccggatccga gctgtgcaaa ctgatccaga taaaaccgtg cggcattgcc
     2041 actgacaaaa acgatcaagt cgtagtcatc cgggaacggg aattcggacc gatggcacgc
     2101 cagcggctga acgaccagtg cgggcagcac cagcgtgctt agtccggcgt cattcaaacg
     2161 caacgccagt ggaccgtttt taccgcccgg acgcgttaaa acaataaggg ggtccatgct
     2221 ttcccaccac agcaagcgtc atctataacg cggttgccag cttgagcaat atagccttgg
     2281 cgcctttgtc gaggagatct tgtccggcct gttcgcccaa ctggcgggca ttctgaacag
     2341 gtcccgatac ttcggagcgc acaatcaaag agccatcagg ttcggcaacc agcgcccgca
     2401 aattaagcac cgcacctttg atttcggcaa aagcggccag cggcacttgg caggaaccgc
     2461 ccaacacgcg cgaaacagcg cgttcggccg tcgcgcaggc ggttgtgtcg gcgctgccta
     2521 gcggcgcaag ccattgttga agatcggtgc gactggccat gatctcgacg gcaagcgccc
     2581 cctgccccgc cgccggccag ctttgctccg gagtcaggta cccgcgaatg cgctgcgaca
     2641 aaccaagccg gcgcaatccc gccgccgcca gcacaatcgc agcataatcg ccccggtcga
     2701 gcttggtcag acgggtatcc agattaccgc gcaatggcag aaccttcaga tgcggataat
     2761 gcacctttat ctgcgcctcg cgacgcaagc tggatgtgcc tacaatcgcg ccagcgggaa
     2821 gctgatcgag cgaatcgaat tcgttggaca ccagcgcgtc gcgcgggtcg tcgcgttcga
     2881 gaacggctgt caatgcgaag ggcggctgca ggtcgaccgg cacgtcttta agcgaatgta
     2941 cggccaaatc ggcgcgacca tccagcagcg ccttttccag ttctttgatg aacaggcctt
     3001 tgccgcccac tttggacaat gtccgatcca ggatctgatc gccgcgggta gtcatcgtaa
     3061 gaagaacgac ttcgcaaccg ggatacagac caatcagccg atcgcgcacg tgttccgcct
     3121 gccataaggc aagccggctg gcgcgcgttg ctataaccag cctgttaggc gcagacaaca
     3181 tgattcagcc cgcgagctta ttcacgataa ccgcggcaat cacgcccaaa atggcaagaa
     3241 tgaacattcc ttgcaatagc gacaggcctg attccgattt tttactgtcc gacgatttaa
     3301 acaatcccat gcagatcttc ctttgcagct tctttcgcgg cgagcttgcg actggcgaac
     3361 cattttccga acataacgac cagaatggcg cccaccgcct cggccatgat tttaaccgga
     3421 acaggaacca cacccaattg atcaacgata aatatatcgg taaccagcat gccgccagca
     3481 atccagccga gcaagccggc ccccaaagta accaccacgg gaaaacgatc gatgagcttc
     3541 agcaccagtg tgctgcccca gacaataatc ggcacgctaa gaagcaaacc gaatatgaca
     3601 tagcccaatt gatggtcggg aagcgtgttt tgcgccgcgc cggcaattgc aataatgtta
     3661 tccaggctca tgacaaggtc ggcaacgata atcgttttaa ccgcggccat gacggaactg
     3721 ccgcccttta ccttgtcgtg atggtcgtcg tcggggacaa gcaacttaat gccgatccat
     3781 agcaataata gtgcacccgc catcttcagg aaaggtacgg tcagaagcgc aagagcaaac
     3841 gcgatcagaa ccacgcgcgt gatgatcgcg ccggcggtgc cccacaaaat tccctgtatc
     3901 cgttgtttgt gctgcaaatt gcgacaagcc agggcaataa caactgcgtt gtcgccaccg
     3961 agcaaaatgt cgatcaaaat aatttggaaa agggcacccc aatgaagggt ttcaatgaac
     4021 tctaacattc agcttccact tacataaagc atgatcaggg aaaccttgat gcgcagcgca
     4081 tcaaggttta tggcagaggc tattgtaatt cagctttcag tcagtttgcg ccctggctat
     4141 gaaaactttc cccaatacaa gcaccacgac agcgccgacc gcccctatgg ccatggcaag
     4201 ctgatctgga gaggagtgca taatgcctga aaaactgtcg acaacacctc gcagcgctgg
     4261 atcgctgacc aacatttcac ccgcaatata acccagcaat gcggcgccaa accatacgat
     4321 gatcggcaaa cgatcaatta ctttcagcag caaggtgctg ccgaagacta ccagcgggat
     4381 gctgattccc agccccacga tcaacagaaa caaatcaccg tgcgccgcag ccgcgacggc
     4441 cacgacgtta tccaggctca tgatcagatc ggcgaccaaa attgtgcgaa tggcccccca
     4501 tatgcttccg tatcgcttgg cctcgccctc atcgtgcgcc tcgggcagga gcaaggtaac
     4561 cccaatgtac accagcaaaa cggcgccgac aatcttgagc cacggcaaca acagcagttc
     4621 ggcagccgcc acagtcaaaa gaatgcgcaa cacaacggcg gcgccggaac ccgcggcaat
     4681 ggcagtgaac tgttgcgacg tcggcaatga ccgggcagcc aatgcgatca cgaccgcatt
     4741 gtcgcccgat aacaaaatgt tgacccagat tatttctaat aaagccagcc aaaaggccgt
     4801 cgaactaaaa tccatgtgtc gttcatccta tccgaagatc ccccttcgtc taagcccgcg
     4861 aacagcttca cctggtcgag tgccagcgtt tagccgctat atgcgag
//
LOCUS       DATCXU010000158         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_25391,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000158 DATCXU010000000
VERSION     DATCXU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25391"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /locus_tag="VIR04_03530"
     CDS             complement(<1..2113)
                     /locus_tag="VIR04_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY9459851.1"
                     /translation="MKWGLRLALVIAVVSALALLALLVWSTGNASRYAQQYDTLLLLN
                     GIFAVALISWVLALSARLLRQIRRRQFGARLTARFALSFALIGVLPGALIYVLSVQFM
                     SRSIESWFNVRVDSALEAGLNLGRAALDSQLADLSARARDIASRLDNTSDSGIALSIT
                     RLREASGVAEAMAFTGNGRLVAFSSSAYGKLLPDMPPPNVMNQLRVSRGYAAAETDAP
                     ADPHKTDATDATDATPASGLHLRVVIPLNNLDRYSSALGMAPDTRWLQVVQPVPERIA
                     HNANQVEQGFRDYQELALSRLGLRKLYGITLTLALVLAVFAAIAVALALSRRLVRPLL
                     TLAAGTQAVGVGDYRPLPEPPEKDEVGQLTRSFNAMTRQLDEARHMVENNRRQLERSN
                     VYLESVLGNLSTGVLVFDEAFLVTMFNQGAQTILRVDLRLVKGRPLETIEGAFELSQL
                     IRKAFAAHTAVGSERLYWQQQFELELEAASDNETKKHIVTLLARGTHLNVDGRGNGYL
                     VVFDDISEIMSANRTVAWGEVARRLAHEIKNPLTPIQLSAERLALKLADRLNATDAAM
                     LARSTTTIVDQVASLKKMVDDFREYARTPPAQMQHIELNRLIADVLALYGWDPVEGAV
                     HDDGKTIKIEVELDPDLPVVEGDPTQLRQVIHNLLANARDAATQDRPSADAKIYVQTK
                     SARAGANGLAERVAVRLTVSDN"
     gene            complement(2110..2724)
                     /locus_tag="VIR04_03535"
     CDS             complement(2110..2724)
                     /locus_tag="VIR04_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY9459852.1"
                     /translation="MIIRAWFSIVLCLFLAVRAQAQAPKAVAAAPAAASGAFDRVVHI
                     DPVVQEGKLYIDADVDFAINSDLRNAAEKGVPVYFTADLQITSRRWWWFDKVEVDQAL
                     TWRIVYNALTRQWRVGTGDLLMSEPSLDDALSLVRHIRGWDVANTSDLEPGRTYHGRM
                     RVRLNTSLLARPFQIDALNSSAWSLATPWKDFSFSISVDAHDPS"
     gene            complement(2773..4086)
                     /gene="rsmB"
                     /locus_tag="VIR04_03540"
     CDS             complement(2773..4086)
                     /gene="rsmB"
                     /locus_tag="VIR04_03540"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY9459853.1"
                     /translation="MLASAQNALEVLTGHSLSDSLAQTPVELRAATQAVTFHAMRRLG
                     LARAVKQILVQRTPPNPLFDALLLVALALLDTALEVAHGAQAGVPPRRDLPVYAAHTV
                     VDQAVRAAAGQRQMQFYKGLLNGVLRRFVRERSAILSQITHKPEAVWNHPAWWVKQLK
                     QSYPEQWEYLLESANRPGPMTLRVNARRASVEQVLDELAGAGVAARAVGGNAIVLALA
                     RPVHALPGFAQGWWSVQDASAQQAAPLLRAGPGMRVLDACAAPGGKAAHLLELADIEL
                     VALDTDQRRLGRVGQNLDRLGLMSSRVSLRCADAADPASWWDGRAFDAVLADVPCTAS
                     GIVRRHPDIRWLRRETDIARTAALQRKIVDALWTTVKPGGRLLYATCSIFPQEGERQA
                     LQFLARHNDASREPAPGQILPTPGIGHEESGFYDGFFYALFFKSA"
     gene            complement(4222..>4395)
                     /locus_tag="VIR04_03545"
     CDS             complement(4222..>4395)
                     /locus_tag="VIR04_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY9459854.1"
                     /translation="QALGQAADKDAGKVQSVSPQGIDIATGDGVLRLLELQKPGGKRQ
                     AVAAFVRGWQAEF"
BASE COUNT          981 a         1419 c         1196 g          799 t
ORIGIN      
        1 cattatccga cacggtcagc cgcactgcga cacgctcggc caggccattg gcgcccgccc
       61 gcgccgactt ggtctgcaca tagattttag cgtctgccga cgggcgatcc tgagtggccg
      121 cgtcgcgcgc gttcgccagc aaattatgaa tcacctgccg caactgcgtc ggatcgccct
      181 cgacaaccgg caaatcgggg tcaagctcga cctctatttt gatggttttt ccgtcgtcgt
      241 gcacagcgcc ctcgaccggg tcccaaccat ataacgccag cacgtcggca atcagccgat
      301 taagttcaat atgctgcatt tgcgccggag gcgtgcgcgc atactcgcgg aagtcatcca
      361 ccattttctt cagggacgcc acctgatcca cgatcgtggt ggttgagcgg gccaacatgg
      421 ccgcatcggt tgcattcaaa cgatcagcca gcttcaacgc cagcctctct gccgataatt
      481 gaattggcgt caggggattt ttaatttcat gggcaagccg gcgcgcgact tctccccagg
      541 ccaccgtgcg gttcgccgac atgatttcgc tgatgtcgtc gaacaccacc agatatccat
      601 tgcctcgccc gtcgacattc aaatgcgtgc cgcgcgccaa taaagtgacg atatgttttt
      661 tggtttcatt gtcgctggcc gcctcgagtt caagttcgaa ctgctgctgc caatacaaac
      721 gctccgatcc cacagccgtg tgcgccgcga atgccttgcg tatcaattgc gacaactcga
      781 atgcaccctc tattgtttca agaggccggc cttttaccag ccgcaagtcg acccgcaaaa
      841 ttgtttgcgc gccctgattg aacatcgtga cgagaaacgc ctcgtcaaac accagaacac
      901 ccgtggacaa attgcccagt acggattcaa ggtaaacatt ggaccgctca agctgacggc
      961 gattgttttc caccatatgg cgcgcctcgt caagctggcg cgtcatggcg ttaaatgagc
     1021 gcgtcaactg gccgacctca tctttttcag gcggctccgg taaaggacgg taatcgccca
     1081 cgcccacggc ctgcgtgccc gccgccagcg taagcagggg acgaaccaga cggcgtgaca
     1141 gggccagtgc aactgcaatc gccgcaaaca cggccagcac aagcgccagg gtcagggtga
     1201 tgccgtacaa cttgcgcagc cccaggcgcg acaaagccag ttcctgataa tcgcggaacc
     1261 cttgttccac ctggttggca ttgtgcgcaa tgcgctcggg caccggctga accacctgca
     1321 gccaacgtgt atccggcgcc atgcccaagg cagaggaata ccggtcaagg ttattgaggg
     1381 gtattaccac gcgcagatgc aaaccgctgg ccggcgttgc gtcggttgca tcggttgcgt
     1441 cggttttgtg cggatcggcc ggcgcatcgg tctcggcggc tgcataaccg cgcgaaaccc
     1501 ttaactggtt catcacattg ggcggcggca tatcgggcaa caacttgcca tacgccgacg
     1561 aggaaaaagc aaccaggcgc ccgttccccg tgaatgccat cgcctcggca accccgctgg
     1621 cctcgcgcag gcgcgtaatg gaaagcgcaa tacccgaatc gcttgtgttg tccagcctgg
     1681 aggcgatgtc gcgcgcgcgg gcgcttaaat cggccaattg tgaatcaagc gccgcgcgcc
     1741 ccaggttcag gcctgcctcc agagcgctgt cgacccggac attaaaccag gactcgattg
     1801 agcgcgacat gaactgcacc gacaacacat aaatcaatgc cccgggcagt acgccgatca
     1861 aggcaaatga caaagcaaac cgggcggtaa ggcgcgcgcc aaactggcgc cgccgaattt
     1921 gacgcaacaa gcgcgccgac agcgccagca cccacgaaat aagcgcaacg gcaaaaatgc
     1981 cattgagcaa cagcaaagtg tcatattgct gtgcataacg cgacgcgttg ccagtcgacc
     2041 agaccagcaa ggccaacaat gccagcgcac tgaccaccgc gatgaccagt gccagccgca
     2101 aaccccactt tatgagggat cgtgcgcgtc gacggagatt gagaacgaga aatctttcca
     2161 tggggtcgcc agtgaccagg cgctgctgtt taatgcatca atttgaaagg ggcgcgccaa
     2221 taaagacgtg ttcaagcgca ccctcattcg gccgtgatac gttcggccgg gttccagatc
     2281 ggacgtgttg gcgacatccc aaccgcgaat atgccgcacc aaagacagcg catcatccag
     2341 cgagggctcg gacattaaca agtctccggt gccgacgcgc cattgccgtg tcagtgcgtt
     2401 ataaacaatg cgccaagtga gcgcctgatc gacctcgacc ttatcaaacc accaccagcg
     2461 cctggatgtg atttgaagat cggccgtgaa ataaacgggc actccttttt cagccgcgtt
     2521 gcgcagatcg ctattaatcg caaaatcaac gtcggcgtcg atgtaaagct tgccttcctg
     2581 cacgacaggg tcaatgtgca ccacccggtc aaacgcgccc gacgccgccg ccggcgccgc
     2641 cgctacagcc ttcggcgcct gagcctgcgc gcgaactgca agaaacagac acaaaacgat
     2701 agaaaaccaa gctcgaatta tcattgcgta tcagcacaaa acagataccg aaaacagcct
     2761 gctttcgacg tctcaggcgc ttttgaaaaa caaggcataa aaaaaaccat cgtaaaatcc
     2821 agactcttcg tggccaattc cgggtgttgg caaaatctgg cccggagccg gttcgcgcga
     2881 cgcgtcgttg tgccgcgcca aaaactgcaa tgcctgccgc tcgccttctt gcggaaaaat
     2941 agaacacgtc gcatacagca atcgccctcc cggtttgacc gtcgtccata gcgcgtcgac
     3001 aatcttgcgt tgcaacgcgg ccgttctggc gatgtcggtt tcacggcgca gccatcggat
     3061 gtcgggatgg cgccgcacga tacccgatgc ggtgcacggc acatcggcca gcacggcatc
     3121 aaacgcgcgc ccgtcccacc acgatgctgg atcggccgca tcggcgcacc gcaacgacac
     3181 acgggacgac atcaatccca gacgatccag attttgcccg acccgcccaa gccgccgctg
     3241 gtcagtatcc agcgccacca attcaatatc cgccagctcc agcaaatgcg ctgccttgcc
     3301 gcccggagcc gcgcaggcgt caagcacccg catccccgga cccgccctta acaacggggc
     3361 ggcttgctgc gccgacgcat cttgcactga ccaccagcct tgcgcgaacc cgggcaaagc
     3421 atggaccggg cgcgccagcg ccagcacaat ggcgttgccg ccaaccgccc gcgctgccac
     3481 cccggcgccc gccaattcgt ccagcacctg ttcgaccgac gcccggcggg cgttcacgcg
     3541 cagcgtcatg gggccgggcc gattggcgct ttcaagcaag tattcccact gttcgggata
     3601 ggactgcttc aactgcttaa cccaccaggc cggatgattc cacaccgctt cgggtttatg
     3661 ggtgatctgg ctcaggattg cgctgcgctc gcgcacaaac cggcgcaaga ctccattcag
     3721 caaacccttg taaaactgca tctggcgctg tccggctgcg gcgcgaacgg cctgatcgac
     3781 gacggtatgg gccgcgtaca caggaagatc ccggcgcggc ggaacccccg cctgcgctcc
     3841 atgggcgact tccagcgcgg tatcaagcaa agccagcgcc accagcaaca aagcatcaaa
     3901 taaaggattg gggggcgtgc gctgaaccaa tatttgcttg accgcccgcg ccaaccccag
     3961 gcggcgcatg gcatgaaatg tcaccgcctg ggtcgcagcg cgcaattcta ccggcgtttg
     4021 cgccagacta tcggacaggg aatggcccgt caacacctcc agcgcgtttt gcgcactggc
     4081 cagcataacg tcggataaca aaggccggga cggcttgaag gggatgtctg gcacgagata
     4141 taaagcctgc ttgaagaaag gttttcatgt ggactttatc accaaatacc acgcgcgcca
     4201 tggcgcgcag cacgatcatc gctaaaattc ggcctgccag ccccggacaa aggcggcgac
     4261 cgcttgcctt ttccctcccg gtttctgcag ttcgagcaaa cgcaatacac catcgcccgt
     4321 ggcgatatcg ataccctgcg ggctgacgct ttgtaccttt ccagcgtctt tatcggcggc
     4381 ctggccaagc gcctg
//
LOCUS       DATCXU010000159         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1136496,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000159 DATCXU010000000
VERSION     DATCXU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136496"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VIR04_03550"
     CDS             <1..426
                     /locus_tag="VIR04_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY9459855.1"
                     /translation="ADTGTKMIHMGRNSRSTIISKGISAGFGSNTYRGMVRVTPKANN
                     ARNYTQCDSLLIGGTCAAHTFPNIEVQNQTARVEHEATTSRIGEDELFYAMQRGLSAE
                     DAVSMIVNGFCKDVFKELPMEFAVEAQNLLTVSLEGSIG"
     gene            442..1212
                     /gene="sufC"
                     /locus_tag="VIR04_03555"
     CDS             442..1212
                     /gene="sufC"
                     /locus_tag="VIR04_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY9459856.1"
                     /translation="MLKISDLHASVGDKPILRGLNLEVNPGEVHAIMGPNGAGKSTLA
                     QVLAGRADFTVNSGTATWEDQDLLSMPIEVRARSGLFLAFQYPIEIPGVSNAYFLKAA
                     VNSARQHRGLPELDAMDFLKKVKAEMKAVGMKPEFLYRSVNEGFSGGEKKRNEVLQMA
                     LLEPKLAILDETDSGLDIDAMKIVAEGVNRLRSPDRALIVITHYQRLLDYIVPDFVHV
                     LAEGRIVRSGGRELALELEKRGYGWIKEELAKQAEAAV"
     gene            1209..2519
                     /gene="sufD"
                     /locus_tag="VIR04_03560"
     CDS             1209..2519
                     /gene="sufD"
                     /locus_tag="VIR04_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY9459857.1"
                     /translation="MSTTQSWMSAYQSRVRALPGAGAPWLAAVRQRAIERFVDEGWPT
                     LKQEGWRTTSLAELEQQNFTTPGAALSPAALIEQLRGNEPGHWLVFVDGRYAPELSAV
                     GKLPDGSQVCALSSVLNGHPEQVEPYFGVEQDGSTPSALNLAMAADGGFVSIGAGRDI
                     DLPINLVFVAVTAQSACFPRNLIIAQAGSHATVVEHYIGSDGGATLTNAVTRAFISAD
                     ANITHLKLQQESDKAFHMAAIDVMQERGSVYNSHSLSFGAHFARNDIETQFRGDGCET
                     LFNGLYYVDGRRHVDHHTLIDHAKPSGSSREFYRGILAGGARGVFRGRIRVAQGADRT
                     DALQRTDSLLLSRLAKADARPELEIYAEDVKCAHGATIGQLDDASLFYLRTRGIEEDR
                     ARSLLTYAFAAEVLGRIEIEPLRKRAAWAIRALLPGGESLEELA"
     gene            2516..3772
                     /locus_tag="VIR04_03565"
     CDS             2516..3772
                     /locus_tag="VIR04_03565"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY9459858.1"
                     /translation="MNAPAFAAKASASLNRTADFPILALRVRGRRLSYLDNGATTQKP
                     LSVINAERDFYLHSNANIHRGVHWLSQHATDLYEAARERVKGLLNARQAKEIVFTRGT
                     TEAINLVAQSWGRSQLKAGDEIILTGMEHHSNIVPWQLVCEQTGAVLRVVSVTDSGEL
                     DMGEFSSLLGARTRLVAVAHVSNALGTVNPIAEIVRLAHAAGAKVLVDGAQAVAHQAV
                     DVQALDCDFYAFSGHKIYGPTGIGALYVRYEILKAMPPWQGGGDMIYTVSFEKSTYAP
                     VPQRFEAGTPNIAGAVGLAAAIDYVQDVGLDNIQAHEQALLAYATPLLMDLPGLRLIG
                     TAANKSGILSFVVEGIHPHDLGTILDTEGVAIRAGHHCAMPLMTRFGIPGTARASLGL
                     YNDENDIDALVAALHKAQEMFAGRAS"
     gene            3769..4221
                     /locus_tag="VIR04_03570"
     CDS             3769..4221
                     /locus_tag="VIR04_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY9459859.1"
                     /translation="MNDHSDLRELYQEVIFDHNRHPRNFHAMADASHSADGYNALCGD
                     RLKVYLQVKDGVVTDASFEGHGCAISTASASLMTETVIGKPVAEVDTLFKDLHNMLTG
                     GPTEEHDFGKLEVLAGVCEFPSRVKCATLAWHTMHNALVGADDTAETE"
     gene            4233..>4736
                     /locus_tag="VIR04_03575"
     CDS             4233..>4736
                     /locus_tag="VIR04_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY9459860.1"
                     /translation="MKYGREEIIVNRDCPAVTIPAGAPVTIEEGCAAQITQQLGGTYT
                     VLVEGNLYRIEGVDGDALGFESVDHGESAGAPEGPITPESVEEAAWTLLSTCYDPEIP
                     IDIVNLGLVYSCKVLDAGENLYRIDIQMTLTAPGCGMGTMIADEARAKLLSIHGVEQV
                     KVDLVWDP"
BASE COUNT          995 a         1254 c         1447 g         1040 t
ORIGIN      
        1 gccgacaccg gcacaaagat gatccatatg ggccgcaatt cgcgcagcac gattatttcc
       61 aaggggatct ccgcgggttt cggcagcaat acctaccgcg gcatggtgcg cgtgacgccc
      121 aaggcgaaca acgcgcgcaa ttacacccaa tgcgactcgc tgcttattgg cggaacctgt
      181 gcggcgcata cgtttcccaa cattgaagtg caaaaccaga cggcgcgggt tgagcacgag
      241 gccacgacgt cgcgcattgg cgaagacgaa ttgttttacg caatgcagcg cggcttgtcg
      301 gctgaagacg ccgtttcgat gatcgtcaat ggattctgca aagatgtttt caaggaattg
      361 ccgatggagt ttgctgttga agcgcagaat ttgctcacgg taagtcttga aggcagcata
      421 ggctaaggag aagtaagaat gttgttaaaa atcagcgatc tgcacgcgtc ggtgggcgac
      481 aaaccgatcc ttcgcggcct gaatctcgag gtcaatcctg gcgaagtgca tgccattatg
      541 gggccaaacg gcgccggaaa aagcacgctt gcccaggtgc tggccgggcg cgccgatttc
      601 acggtcaata gcggcacagc cacttgggaa gaccaggatc tattgagcat gccaattgaa
      661 gtgcgggcgc gctccgggtt gtttcttgcg tttcagtacc cgatcgaaat cccgggcgta
      721 tcgaacgctt attttcttaa ggcggccgtg aattcggcgc gccagcatcg cggcctgccc
      781 gaactggacg caatggattt tctcaagaag gtaaaagccg agatgaaagc ggtgggcatg
      841 aagcccgaat ttttgtaccg ctcagtcaat gaaggctttt cgggcggcga aaaaaagcgg
      901 aacgaagtgc tgcagatggc cttgcttgaa ccgaagctgg ccatcctgga cgaaacggat
      961 tccggtctgg atatcgatgc catgaagatt gtggccgaag gggtcaaccg attgcgctcg
     1021 cctgatcgtg cgcttattgt cattacgcac tatcagcgct tgcttgatta tattgtgcct
     1081 gattttgttc atgttctggc cgaaggccgc attgtgcgca gcggtggccg cgaattggct
     1141 ctggaacttg aaaagcgagg ttacggctgg atcaaggaag agctggcaaa acaggctgag
     1201 gcggcggtat gagcacgact caatcctgga tgtcggccta tcagtcacgc gtgcgcgcac
     1261 tgccgggcgc gggtgcgcca tggctggccg cggtgcgtca gcgcgccatc gaacgttttg
     1321 tcgatgaagg gtggccaacg ttaaagcagg aaggctggcg cacgacgtcg ctggctgaac
     1381 tggagcagca gaactttacg acgcccggcg cggcgttatc gcctgcggcg ctaatcgaac
     1441 agttgcgcgg aaacgagccg gggcattggc tggtgtttgt tgatggacga tacgcgcccg
     1501 agttgtccgc cgtcggaaaa ctgcctgatg gcagccaggt ctgcgctttg tccagtgtat
     1561 tgaatgggca ccccgagcag gtcgaaccgt atttcggcgt tgagcaagat ggctcgacgc
     1621 cctcggcgtt aaatcttgcc atggctgccg acggtggttt tgtcagcatt ggggcgggac
     1681 gcgacattga tcttcccatc aatctggttt ttgtggccgt caccgcgcaa agcgcgtgtt
     1741 tccctcgcaa cctgattatt gcgcaagcgg gctcgcacgc gacggtggtc gaacattaca
     1801 tcggttctga tggcggcgcg accctgacca atgccgtgac gcgcgctttt attagtgcgg
     1861 acgcaaacat cacccacttg aagttgcagc aagaaagcga taaagccttt cacatggccg
     1921 cgatcgacgt catgcaggaa cgcgggtccg tttataactc gcattccctg tcgtttggcg
     1981 ctcattttgc gcgtaacgac atcgagactc agttcagggg cgatggctgc gaaaccttgt
     2041 ttaacggctt gtactacgta gacgggcgtc gccatgtcga tcatcacacc ttgattgacc
     2101 atgcgaaacc atcgggctcc agccgcgagt tttatcgcgg cattcttgcc ggtggcgcgc
     2161 gcggcgtgtt tcgtggccgt atccgcgttg cgcaaggcgc tgatcgcacc gacgcgttgc
     2221 agcgcaccga tagtttgctg ttgtcgcggc tggccaaggc cgatgcccgg cccgagctgg
     2281 aaatctatgc cgaggacgtc aagtgcgcgc acggcgcgac catcggacag cttgatgatg
     2341 ccagcctgtt ttatctgcgt acgcgcggca ttgaagaaga ccgtgcgcgc agtttgttaa
     2401 cgtatgcatt cgcggccgaa gtcttgggac gtatcgagat agagccattg cgcaagcgtg
     2461 cggcatgggc catacgcgcc ttgctcccgg gcggagaatc tctcgaggag ctcgcatgaa
     2521 cgcgcctgca tttgccgcca aagcgtcggc cagcctgaac cggactgccg attttccgat
     2581 tttggcgctt cgtgtgagag ggcggcgcct gagctatctg gacaacgggg ccaccactca
     2641 aaaaccgtta tcggtgatta atgccgagcg cgatttctat ctgcactcca acgccaacat
     2701 tcatcgaggc gtccattggc tctcgcaaca cgccaccgat ctgtacgagg cggcgcgcga
     2761 gcgggtaaaa ggtttgctca acgcacgcca ggccaaagaa atcgtgttca ctcgcggcac
     2821 taccgaggca attaatctgg tggcgcaaag ctggggccgt tcgcaattga aggcaggcga
     2881 cgaaatcatt ctcaccggca tggagcatca ctccaatatt gttccatggc agcttgtttg
     2941 cgagcaaaca ggagcggtgc tgcgcgtcgt ttcagttacc gatagcggcg aattggatat
     3001 gggcgagttt tcctcgttgc tgggggcgcg cacgcgcctg gtagcggtgg ctcatgtgtc
     3061 caacgctttg ggtacggtaa acccgattgc tgaaatagtt cgtctggcgc atgccgccgg
     3121 cgccaaggtt ctggtcgacg gggcccaggc ggtggcgcac caagctgtgg atgtgcaggc
     3181 gctcgattgt gacttttacg cgttctccgg ccataagatt tatggcccga caggcatcgg
     3241 cgcattgtat gttcgttacg aaatattgaa ggcaatgccg ccttggcaag gcggcggcga
     3301 catgatctac accgtcagct tcgagaaatc cacctacgct ccagtgcctc aacggttcga
     3361 ggcgggcacg cccaatattg cgggagccgt cgggctggcc gccgctattg attatgttca
     3421 ggatgtcggt cttgacaaca ttcaggctca cgaacaggca ttgctcgctt atgcgacgcc
     3481 gttgcttatg gatttgccgg gcttgcgtct aattggcaca gcggcaaata agtcaggcat
     3541 tttgtcgttt gtcgtggaag gcattcatcc gcatgacctg ggcaccattc tggatacgga
     3601 aggcgtggcc atacgggcag gccatcactg tgcaatgcct ttgatgaccc gttttggaat
     3661 ccccggcacg gcacgcgcgt ccttaggcct gtataacgat gaaaacgata tcgatgcgct
     3721 tgtcgcggcg ctgcataagg cgcaggaaat gttcgcgggg cgtgcgtcat gaacgatcat
     3781 tctgatttgc gcgagctcta tcaagaagtc attttcgatc acaaccgcca tccacgaaat
     3841 tttcacgcca tggctgatgc cagccattcg gccgacggat acaacgcgct atgcggcgat
     3901 cggctcaagg tttatttgca ggtgaaagac ggcgtggtca cggacgccag ttttgagggc
     3961 catggctgcg ccatttcgac ggcttccgcc tcattgatga ccgaaacggt gattggcaag
     4021 cccgttgccg aagtggatac tttattcaag gacctgcaca acatgttgac gggcgggcca
     4081 actgaagagc acgattttgg caagcttgaa gtgcttgcgg gggtttgcga gtttccgtcg
     4141 cgcgtgaaat gcgcaacgct ggcttggcac actatgcata atgcgctggt gggcgcggac
     4201 gacacggctg aaacggaata aaaggcgttt tcatgaaata cggacgagaa gagatcattg
     4261 tcaatcgcga ttgcccagcg gtgactattc ctgcaggcgc gcctgtcacc attgaagagg
     4321 gctgcgcggc gcaaattact caacaattgg gcggcactta caccgttctg gttgaaggca
     4381 atctttatcg aatcgaagga gtcgatggcg atgccttggg tttcgagtcc gtggatcatg
     4441 gcgaaagcgc gggcgcgcct gaggggccga ttacgcccga gtcggtcgaa gaggccgcat
     4501 ggacgcttct gtccacatgc tacgacccgg agatccccat tgacatcgtc aatctgggcc
     4561 tggtttatag ctgcaaggtg cttgatgccg gcgagaatct ttatcgcatt gacatccaaa
     4621 tgacgttgac ggcgccgggt tgcggcatgg gcaccatgat cgccgacgaa gcgcgcgcca
     4681 agttgctatc gattcatggc gttgaacaag tcaaggtcga tctggtgtgg gacccg
//
LOCUS       DATCXU010000160         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_156756,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000160 DATCXU010000000
VERSION     DATCXU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156756"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1255)
                     /gene="wecB"
                     /locus_tag="VIR04_03580"
     CDS             complement(80..1255)
                     /gene="wecB"
                     /locus_tag="VIR04_03580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY9459861.1"
                     /translation="MKIITVVGARPQFVKVAAISRAIAMDPTLDLEDILVHTGQHFDA
                     GMSQVFFAELGIAAPKFNLGISGGSHGAMTARMLESIEIVLTREKPHWVLVYGDTNST
                     LAGALAASKLNIPIAHIEAGLRSFNRRMPEEINRIVTDHLSSLLFCPSNTSVANLGRE
                     GLSDNVHLVGDVMYDMALHFGYQTKAQSATLKRFDLVPKAYVLATCHRAENTDSMHNL
                     AEIISGLAGIAKLKSVIFPIHPRTKAKLREYGLQDKLGNIKIVEPLSYLEMAALEKNA
                     EVIITDSGGVQKEAFFFRVPCITLRNETEWTETVNDGWNCLVGSDQNQMIQAFINRGK
                     QKRQNTCPYGTGNAAKRILDILLKAPLGKSKLALKTETARQSQAQPWPGRQTGVPST"
     gene            complement(1252..3018)
                     /locus_tag="VIR04_03585"
     CDS             complement(1252..3018)
                     /locus_tag="VIR04_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY9459862.1"
                     /translation="MNKIVTNLPKPGWKSTVISNSRELLLKGAPYLDMEQQTVQAMAN
                     DWPDDLGDAAQLRQFLRRYSGFYSVVYSLPGQVVASVDRIRSIPLFYGVANSQFYLSD
                     NAEWVRQQVGDEFTDPVAREELQLVGYVTGQDTLFPQVKQLQAGECLIAEQSGGEINV
                     RCQRYHRYLHTEPKQYDEQALQQQFDHVVVKVVRRLIDYAAGRQIVIPLSGGYDSRLV
                     ATQLKRLGYDNVLTFTYGVAGNKDSQYSKQVADTLGFKWHFVEYTPELWRSTWESAEG
                     WRYQSWASGWGSLAHEQDWPAVKMLKQEGVVSADAVFAPGHIVISFIGGRYSPVEALR
                     KLILTQTGTAAELYKHHYCLAPQNVSTHPPRFWQKRITSRIAQHEGADNVNSPISFDC
                     DVEEWCWQERQAKYTVNSVRVYEFFNYDWWLPFWDKDFFEFWREAPFALRKGREWYVN
                     YSKAQYASQTGAKETTMLGNACESRLRIAIGTLVKKLPHFVWNIHVGLPKWVRIARII
                     KEDASALDQIGMFPRSKTKWLLKNGYTRYGIFAYFFLESIKLNVECRPALPTTHGSAN
                     STKPANDKLTAINGSVGWENHL"
     gene            complement(3015..4460)
                     /locus_tag="VIR04_03590"
     CDS             complement(3015..4460)
                     /locus_tag="VIR04_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY9459863.1"
                     /translation="MIAKPSAIIRKILPKNQFARGVSILVGGTAGSQLLAICAAPVVT
                     RLFSPADFGVLAVYAGLLGLFTVIASLRYELAILLPEDNQEAADTATLSLLVVVAVSV
                     LSIIIVFLGDESIPTLLGVPVLSHYFWLLPIGLLLVGIYQVFNYWALRTKNFTVITGA
                     RLKQSLTSIAIQLLGYPLGPVALLISHAAGQGMGVFSLGKAALRYPQFRHVSFSGIVA
                     AAKRYRQFPIYSTWAGLLNSGGNQLPSLMFAALFSPAAAGLYALTSRVLNMPMIMIGS
                     AIGNVFFTNLAEAHREKRLPQLVAAVQEKLAHVVMPPTLVLLIAGPQLFAFVFGESWR
                     EAGIFSQWMAPWLYVVFITSPLGALFDVLERQQQELIFETALFITRIATIAVGALIGD
                     LITTVALFSLGSMLCWLGYLLWITVSSGNPARSLIKPTVTALAWGALCMTPLALSVWL
                     PLNRNLWFYGLALTGVLVGMRLYFSFYKEQP"
     gene            complement(4495..5613)
                     /locus_tag="VIR04_03595"
     CDS             complement(4495..5613)
                     /locus_tag="VIR04_03595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY9459864.1"
                     /translation="MIDFIDLNEQQIRIKDGIDARIQRVLAHGKYILGPEVDELEQKL
                     AAFAGVKHCITCANGTDALQIAQMALGIGAGDEVITPGFTYIATAETVVLLGAKPVYV
                     DVNPNTYNLDPTLLENAITPRTKAIIPVSLYGQCADMDAINAIARTHGLPVIEDAAQS
                     FGASHNGRRSCNLSTIACASFFPSKPLGCYGDGGALFTNDDELATVLRQIARHGQDRR
                     YHHVRVGANSRLDTLQAAILLCKLEIFEEEIQLRRRVARTYDHLLNQAGITSTPYIEA
                     GNSSVYAQYTIQVPEREKVQTMLRQAGIPTAVHYPIPLNRQPAVADAGAQLPVGDRIA
                     QRVISLPMHPYLAEEVQSTIASTLKSVQAESPDCVSTS"
     gene            complement(5661..>5928)
                     /locus_tag="VIR04_03600"
     CDS             complement(5661..>5928)
                     /locus_tag="VIR04_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY9459865.1"
                     /translation="KGATLGANCTIVCGVTIGEFSFVGAGALINKDVPAYALMVGIPA
                     RQIGWMSEFGEQLALPLSGQAEALCPHTGARYLLQGTHLRRRNA"
BASE COUNT         1340 a         1568 c         1606 g         1414 t
ORIGIN      
        1 gccagccgtt ttttctggct gggcctcgat gtcgtttgta aagaggatat tcattagggg
       61 ccttgattgg tggcgctgtt tacgtgctgg gaacgccagt ctgtcgacct ggccacggct
      121 gcgcctggct ctggcgcgct gtttctgttt ttaatgcgag cttgcttttt cccaatggag
      181 ccttcagaag gatgtccaaa atgcgcttgg cggcatttcc cgttccgtac gggcatgtat
      241 tttggcgttt ctgcttgccg cgattgatga atgcctgaat catttggttt tgatcgctgc
      301 cgacgaggca gttccatccg tcgtttaccg tttcggtcca ttcggtttcg ttccgtagcg
      361 ttatgcaggg caccctgaag aaaaacgcct ctttttgcac tccgcccgaa tcggtaataa
      421 ttacctcggc gtttttctcc agcgccgcca tttccaaata agagagaggt tcaacgattt
      481 ttatgttgcc cagtttgtcc tggaggccat actcccgcag ttttgctttg gttcgagggt
      541 ggatcggaaa tatcacagac ttcagctttg cgatgccggc aagtccgctg ataatttcgg
      601 caagattgtg catgctatcc gtattttccg cgcgatgaca tgtggcgagc acataggctt
      661 tcggcactaa gtcaaagcgt ttcagggtag ctgactgagc ctttgtctga tatccgaaat
      721 gcagggccat gtcgtacatg acgtcgccga ccagatgcac attgtcggac aggccttctc
      781 tgccaagatt tgcgacggat gtattgcttg ggcaaaagag cagcgaggat aagtgatccg
      841 tcacaatgcg gtttatctct tcgggcatcc ggcggttaaa tgatctgagg ccagcttcga
      901 tgtgcgcgat cggaatattt aatttgctcg ctgccagtgc gcccgcaagt gtggaattgg
      961 tatccccata gaccaagacc caatgcggct tttcccgcgt cagcacaatc tctatggatt
     1021 ccagcatcct ggccgtcatg gcgccgtggc tgccgcctga tatgcccagg ttgaatttgg
     1081 gcgcggctat ccccaattcc gcgaaaaaga cctgggacat ccctgcgtca aagtgctgtc
     1141 ccgtatgcac caaaatgtct tccagatcaa gtgtcggatc catggcgatg gcacgtgaaa
     1201 ttgcggccac cttgacgaac tgcggccgcg cgccgactac ggtaataatc ttcatagatg
     1261 attttcccag cctacggagc cattaatagc cgtcagttta tcgttggctg gcttggtgct
     1321 attcgcggag ccgtgcgtgg ttggcagagc cgggcgacat tcgacattca acttgattga
     1381 ttcgagaaaa aaataagcga atatgccgta tctggtgtaa ccatttttaa gcagccattt
     1441 tgttttagag cgcggaaaca taccaatttg gtcgagtgca gaggcatctt ctttaattat
     1501 ccgggcaatg cgcacccact tcgggagacc tacatggata ttccatacaa aatgtggcag
     1561 cttttttacc agcgttccta tggcaatacg caagcggctc tcgcaggcgt tgccgagcat
     1621 cgtggtttct ttggctcctg tttgcgacgc atactgcgct ttcgagtagt tcacatacca
     1681 ttcgcggcct ttccgaagcg cgaacggcgc ttctcgccag aactcgaaaa agtctttgtc
     1741 ccagaagggc agccaccaat cgtaattaaa gaattcgtag acgcgaaccg aattcactgt
     1801 gtattttgcc tgccgctcct gccaacacca ttcctcaacg tcgcaatcaa agctaatcgg
     1861 cgaattgacg ttgtccgctc cttcgtgctg cgctattcga cttgttattc gtttttgcca
     1921 aaaacggggc ggatgtgttg agacgttttg aggagcaagg caatagtgat gcttgtaaag
     1981 ctcggcagcg gtgcccgttt gagtcagaat taattttcga agggcctcaa ccggagagta
     2041 gcgaccgcca atgaaagaga taacgatatg tcccggagcg aatacggcgt ccgcagaaac
     2101 gaccccttct tgtttcaaca ttttgaccgc cggccaatct tgctcgtgag caaggcttcc
     2161 ccagccggag gcccaactct gatacctcca gccttcggcg ctttcccatg tactgcgcca
     2221 tagttcaggg gtatattcga caaagtgcca cttgaaaccc agcgtgtcgg ccacctgttt
     2281 gctgtattgc gaatccttgt tccctgccac gccgtaagtg aaagtaagga cattgtcata
     2341 acccaggcgt ttgagttgcg tcgcgacaag cctggaatcg tacccgccgc taaggggaat
     2401 cacaatctgg cggccagcgg cgtaatcaat caggcggcgt actaccttga ctactacatg
     2461 atcgaactgt tgttgcaatg cctgttcgtc atactgctta ggttcagtgt gcagatagcg
     2521 atggtagcgt tgacaacgca cattgatctc gcctccgctt tgctccgcga tcaggcattc
     2581 tccggcctgc agttgtttga cttgcggaaa caaggtgtct tgcccggtga cgtagcctac
     2641 aagttgaagt tcttcccggg ctacaggatc cgtgaattca tctcccacct gctggcgcac
     2701 ccattcagca ttatcactga gataaaactg gctattggcc acaccgtaaa ataaaggaat
     2761 agaccgaatg cggtccacgc tggcgaccac ttgacccggc agactgtata ccaccgagta
     2821 gaaaccgctg tagcgccgca agaactgccg taattgggct gcgtcgccca agtcatctgg
     2881 ccagtcattt gccattgctt gtactgtttg ttgctccata tccaggtacg gagcgccttt
     2941 taaaagtagt tctcgcgagt ttgaaattac agttgatttc cagccgggtt ttggcagatt
     3001 agtcacgatt ttgttcatgg ctgttccttg tagaacgaaa aatagaggcg catgccgact
     3061 aacacgccgg ttagggcaag cccgtaaaac cataggttgc ggttcagcgg aagccatacg
     3121 ctaagtgcaa gcggggtcat gcataaggcg ccccacgcta gcgccgtaac ggttggcttg
     3181 attagcgaac gtgccgggtt gcccgaagac actgtgatcc agagcaggta tccaagccag
     3241 caaagcatgc tgccgagcga gaacagcgcg actgtggtta taagatcgcc gataagcgct
     3301 cccacagcga tagtggcaat gcgcgttatg aacaaggcgg tttcgaaaat gagctcctgc
     3361 tgctgccttt ccagcacgtc gaacagagct cccagcggag aagtaatgaa cacgacatac
     3421 agccatggag ccatccactg agagaatatg cctgcctcgc gccagctttc gccaaataca
     3481 aaggcaaaaa gctgcggccc cgcaatcaga agaaccagcg tcggcggcat gactacatgc
     3541 gcaagttttt cttgtactgc cgccaccaac tgaggaaggc gtttttcccg atgagcctcg
     3601 gcaagattcg tgaaaaaaac attacctatg gctgaaccga tcatgatcat cggcatgttc
     3661 aacaccctgc tggtcaaggc atataaaccc gccgcggcag ggctgaagag tgccgcgaac
     3721 ataagcgacg gcaactgatt tccgccggag ttgagcagcc cggcccaagt cgaataaatc
     3781 ggaaattgcc gatatcgctt tgccgcagcc actattcccg agaaagacac atgacgaaat
     3841 tgggggtatc gcagcgctgc ctttcccagt gaaaaaacgc ccatcccttg ccctgcggca
     3901 tgactgatca gcagcgccac aggccctagc ggatagccca gaagctggat ggcgattgag
     3961 gtcaatgatt gttttaagcg agcgccggta atgacggtga aattttttgt gcgaagggcc
     4021 cagtaattga aaacctggta gatgcctacc agcagcaatc caatgggcaa taaccaaaaa
     4081 tagtgcgaga gaaccggaac gccgagcagg gttggaatgg attcatcacc caggaacacg
     4141 atgattatac tcagtacact gactgccact acgacgagta gcgacagtgt cgcggtgtcg
     4201 gcggcctcct ggttatcttc tggaaggaga atggccagct cgtaacgcag actcgcgata
     4261 actgtgaaca atccaagaag cccggcatac accgccaaca caccaaaatc ggcgggagaa
     4321 aaaagacgcg tcaccaccgg tgcggcacat atcgccagta attgggagcc ggccgttcct
     4381 ccaactaata tgcttacgcc tcgcgcgaat tgatttttcg gcaagatttt tcgaattatt
     4441 gccgacggct tcgcgatcaa gtttttgctc cttgcaagcc aatcaatatt gcagtcaaga
     4501 agtggaaaca cagtcgggcg attcggcttg aacacttttc agtgtgcttg cgatcgtcga
     4561 ctgcacctcc tcggccaggt agggatgcat gggcagactg atgactcgct gggcgattcg
     4621 atcccccact ggcaattgcg cgccggcatc ggcgacggcg ggttgcctgt tcagtggaat
     4681 cgggtagtgg acggcggttg gaatgccggc ttgtcgcagc atggtttgga ctttttcgcg
     4741 ctcgggcacc tggatcgtgt attgggcata gacgctgctg ttgccggctt caatataggg
     4801 cgtggacgtg atgccggcct gattcagaag gtgatcgtaa gtacgagcga cgcgccggcg
     4861 cagttggatt tcttcttcaa aaatttcaag cttgcaaagc agtatggcgg cttgcaacgt
     4921 gtcgaggcgg ctattggcgc ctacccggac atggtgatag cggcgatcct ggccgtgacg
     4981 ggcaatctgg cgtaaaaccg ttgccaattc atcgtcgttg gtgaacagcg cgccgccatc
     5041 gccatagcag cccagtggct tactgggaaa aaaactggcg catgcaatag tgctaaggtt
     5101 gcacgaacgc cggccattgt ggctggcgcc gaagctttgc gcagcgtctt cgatgacagg
     5161 caggccgtgt gtgcgggcga tcgcgttgat ggcgtccata tccgcgcact ggccatacag
     5221 agagaccgga ataatcgcct tagtgcgtgg cgtgatggcg ttttcgagca gagtcggatc
     5281 caggttatag gtattcgggt ttacatcgac atagaccggc ttcgcgccca gaagcaccac
     5341 agtttccgcc gtggcaatgt aggtgaatcc gggcgtgatg acttcgtcgc cggcgccaat
     5401 acccagcgcc atctgggcaa tttgcagggc atcggtgccg ttggcgcagg taatacagtg
     5461 tttgacgccg gcaaaggcgg cgagcttttg ttccagttcg tcgacttcag gaccaagaat
     5521 gtacttgccg tgggccaata cacgctggat gcgcgcgtcg ataccgtctt taatgcgtat
     5581 ttgctgctcg ttaagatcaa tgaagtcgat catcggaact ccttagaaat tccggcgcct
     5641 tggccaggaa agtgtcatgc ttacgcgttt cgtctgcgca gatgtgttcc ctgcagcaag
     5701 tagcgcgcgc ccgtgtgcgg gcaaagggcc tctgcctgac cacttaaagg cagcgccaat
     5761 tgttcgccga attcgctcat ccatcctatt tgccgcgccg gtatgcccac catcagcgcg
     5821 taggctggaa cgtctttatt aataagcgct ccagccccca caaaggaaaa ttcgccgatc
     5881 gtgacaccgc atacgattgt gcaattggcg cccagcgtgg cgcccttt
//
LOCUS       DATCXU010000161         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_489403,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000161 DATCXU010000000
VERSION     DATCXU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489403"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VIR04_03605"
     CDS             complement(<1..905)
                     /locus_tag="VIR04_03605"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY9459866.1"
                     /translation="MTGNRVETDSMGKIDVPAGHYWGAQTQRSIQNFPIGVVRFKWQP
                     PIIRALGILKKAAAQANAELGELPANIAALIVRAADEVIAGKHDTEFPLVVFQTGSGT
                     QSNMNANEVISNRAIELAGGVMGSKTPVHPNDHVNRGQSSNDTFPTAMHIAVAQELAG
                     QLFPAVGKLRTTLAHKAEQYRDVVKTGRTHLQDATPITLGQEISGWVAQLDFALDAIQ
                     SNLPGVYDLAIGGTAVGTGLNAHPRFGEVAAAHIAEIAGLAFRSAPNKFFALSAHDAL
                     VNLSGSLRTLAAALMKIANDVRWLA"
     gene            complement(940..1965)
                     /locus_tag="VIR04_03610"
     CDS             complement(940..1965)
                     /locus_tag="VIR04_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEY9459867.1"
                     /translation="MSRPVRVAINGYGRIGRCFLRALYESPLQGRFDVVAINEPADLD
                     SMVYLTRFDSTHGIFPGTIEQGDACLIVNGKKIPVSHGPAPEDVDWKSLDFDFLIECS
                     GHYGKRVDLNRFLQAGCPRLLLSHPGASGAEVDLTVVYGINDQKIRGTELLVSNASCT
                     TNAIVPILDQLHGAYGVEHAFLTTLHSVMNDQPLIDGYHHSDLRRTRSAMQSIIPVST
                     GLAQGVERLLPYMAGRVQAKAIRVPTLNVSAIDLVVNLARDVTVQDISALMTKAATRQ
                     PDLLAYATSPRASVDFNHDRHSAIVDGSQIRTNGAGFANLLVWFDNEWGFANRMLDVL
                     KVWSERR"
BASE COUNT          485 a          568 c          526 g          427 t
ORIGIN      
        1 cttgccagcc agcgcacatc attggctatt ttcatcaaag ccgcagccaa agtgcgcagc
       61 gagcccgaca agttaaccag tgcatcgtgc gccgacagag caaaaaactt attcggcgcc
      121 gagcgaaaag ccaaaccagc aatttcggcg atatgggccg ccgccacttc gccgaaacgc
      181 gggtgcgcgt tcaggcctgt gccaaccgcc gtgccgccta tcgccaggtc ataaacgccg
      241 ggcaaattgg actggatggc gtccagcgcg aaatcgagct gagccaccca gccgctgatt
      301 tcctgaccca atgtaatcgg cgtggcgtct tgcaggtggg tgcggcctgt tttaacgaca
      361 tcccgatact gctcggcctt gtgagcaagt gttgtgcgca attttcccac cgcggggaat
      421 aactggccgg ccagttcctg tgccaccgcg atgtgcattg cggtcggaaa tgtatcgttg
      481 gacgattgtc cgcgattgac atgatcattg ggatgcaccg gagtcttgct tcccatgact
      541 ccgcccgcca gctcaatggc gcgattcgag atgacctcgt tcgcattcat attcgattgc
      601 gttcctgacc cggtctgaaa cacaaccagc ggaaattcag tgtcgtgctt gccggcaatg
      661 acttcgtcgg cggctcgcac aatgagagcg gcgatattgg caggaagctc gcccagttcg
      721 gcatttgctt gcgcagccgc cttttttagt atccccagcg ctctgataat gggcggctgc
      781 cacttgaacc ggacaacgcc aatgggaaaa ttttgtattg agcgctgagt ttgcgctccc
      841 cagtaatgtc cagccggcac gtcaatcttg cccatggaat cggtttcaac acggttcccc
      901 gtcataaatg tgctcctcaa tgcaaaaata aaataatgtt caacgccgct cggaccagac
      961 cttgagaacg tccagcatgc gattcgcgaa accccactcg ttatcgaacc agacaagcaa
     1021 attggcaaat ccggctccgt tggtgcggat ctggcttcca tccacaatgg ccgagtgccg
     1081 gtcatgatta aaatcgacag aagcgcgggg actggtcgcg tatgccagca aatcgggctg
     1141 ccgggtcgcg gcctttgtca ttaacgcgct gatatcctga accgtgacat cgcgcgcaag
     1201 gttcacgacc agatcaatag cggaaacatt aagcgtaggc acacgtatgg ccttggcctg
     1261 tacgcggccc gccatataag gaagcagacg ctcgacgcct tgcgcgaggc ctgtcgacac
     1321 cggaatgatc gactgcatgg ccgaacgtgt tcgacgcagg tcggagtgat gatagccatc
     1381 aatgagcggc tggtcgttca tgacagaatg caatgtcgtc aaaaacgcat gttcaacgcc
     1441 ataagcccca tgcaattgat caaggatggg aacaatcgca ttcgtggtgc acgaagcatt
     1501 cgaaacaaga agctccgtac cccgaatttt ctggtcgtta ataccatata caacggttaa
     1561 atcgacttca gcgccactgg cgcccggatg tgaaagcaac aaacgcggac aacccgcctg
     1621 aaggaaccga ttcagatcga cgcgctttcc gtagtggccc gagcattcga tcaaaaaatc
     1681 aaagtccagg cttttccagt ctacatcctc aggtgcgggt ccgtgactga cagggatttt
     1741 tttcccattg acgatcaggc aggcgtcgcc ctgttctatt gtgcctggaa agatgccgtg
     1801 agtggaatcg aaacgcgtca ggtaaaccat gctgtcaaga tccgccggct cgttgatggc
     1861 aacaacatcg aacctgcctt gcaatggcga ttcgtacaga gcgcgtaaaa aacaccggcc
     1921 gatgcggcca taaccattaa ttgctacccg taccggacga ctcatgcaac actcgataat
     1981 aattttttcg ccaaatcagc catgaa
//
LOCUS       DATCXU010000162         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_424491,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000162 DATCXU010000000
VERSION     DATCXU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424491"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VIR04_03615"
     CDS             complement(<1..744)
                     /locus_tag="VIR04_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY9459868.1"
                     /translation="MDAKADLTKLALDYHAYPVAGKISVVPTKPLANRDDLSLAYSPG
                     VAAACMAIFDQGEEAAAKYTSRSNLVGVVTNGTAVLGLGNIGPLASKPVMEGKACLFK
                     KFAGIDVFDIELNETDPDKLVEVIAALEPTLGGINLEDIKAPECFYIEKKLRQRMKIP
                     VFHDDQHGTAIISSAAITNGLKVVNKKIADVKLVCSGAGAASIACLDLLVHMGIKQSN
                     IYVVDSRGVIWEGRDKAMEPNKARYAQTTD"
     gene            1043..3913
                     /locus_tag="VIR04_03620"
     CDS             1043..3913
                     /locus_tag="VIR04_03620"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY9459869.1"
                     /translation="MLSEIELQSNSYLFGGNAPYVEELYELYLDNPGSVPDRWRSYFD
                     QLQHLPASDGLEATRDQAHAPIIESFAQRARNNGFVARAQEPDLAVAGRQVHVQTIIA
                     AYRSMGSRLADLDPLKRQDRPVIPELDPSSYGMLESDLDQVYSATNTYFTKADTMTMR
                     DIIKALRDTYCRSVGAEFMYITDPAAKRWIQERLESTLGVPSYSNDRKRYILRQVTEA
                     EGLERFLHTKYVGQKRFSLEGGESFIAAMDEVVGHAGDNGVQELVVGMAHRGRLNLLV
                     NIMGKMPADLFAEFEGKHAEGLTDGDVKYHNGFSSDLSTRGGPVHLSLAFNPSHLEIV
                     NPVVEGSVRARQDRRGDAEGVQVLPVLVHGDAAFAGQGVIMETLNLAQTRGFGTGGTL
                     HIVINNQIGFTTSDPRDTRSTLYCTDVVKMIEAPVFHVNGDDPEAVVYVTQLALDYRL
                     KFRHDVVVDIVCFRKLGHNEQDTPSLTQPLMYKSVGKHPGTRKVYADKLVAQGVLAEG
                     EPDQMVKDYRQLMEDGQRTVEPVLTDYKSKYATDWSSFLGAKWTDQADTGVPLAELTR
                     IGERITTIPENFTVHPLVHKLLDDRRSMIAGERPLDWGMAEHLAYATLLAAGYPIRIT
                     GQDAGRGTFSHRHAVLHDQKRERWDGGAYIPLQNVSEGQASFTIIDSVLSELAVLAFE
                     YGYAIAEPNALTIWEAQFGDFANGAQVVIDQFVTSGEAKWGRHCGLTMMLPHGYEGQG
                     PEHSSARIERFLQLCADNNIQVVQPTNASQIFHVLRRQMIRQFRKPLIIFTPKSLLRN
                     KGAASPMADLANSQFLPVIGETDETIDPAAVTRVLVCSGKVYYDSLHARAEAGRTDVA
                     VLRIEQLYPFAHKSFQTELRKYANAKEVIWVQDEPQNQGAWFYIQHHLYENMVEGQKL
                     GYAGRAASASPAVGYLAKHQEQQRNLHEQALAPKFKGVMFTK"
     gene            3943..>4186
                     /locus_tag="VIR04_03625"
     CDS             3943..>4186
                     /locus_tag="VIR04_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY9459870.1"
                     /translation="MAIIDVLVPQLSESVSEATLLTWKKKPGEAVQADEILIEVETDK
                     VVLEVPAPNSGVLAEVLKGDGSTVLAGEVLGRIDTEA"
BASE COUNT          906 a         1154 c         1151 g          975 t
ORIGIN      
        1 atcggtggtt tgcgcatagc gcgccttgtt gggttccatg gctttgtcgc ggccttccca
       61 gatcacgccg cgcgagtcga ccacatagat attggattgc ttgatgccca tgtgaaccag
      121 caggtccagg cacgctatcg aggcggcgcc cgctcccgag catacaagtt ttacatcggc
      181 gatttttttg ttgacgactt tcaatccatt ggtgatcgcg gccgaagaga taatggccgt
      241 gccatgctga tcatcgtgaa agaccggtat tttcatgcgc tggcgcagct ttttttcgat
      301 ataaaagcat tcgggtgctt taatgtcctc gaggtttatt ccgcccagcg tgggttccag
      361 cgcggcgata acttcgacca gcttgtccgg atcggtttcg ttcaactcaa tatcgaacac
      421 gtcgattccg gcgaattttt taaacaggca cgccttgcct tccatgaccg gctttgatgc
      481 cagcggccca atattgccca aaccgagcac cgccgtgcca ttggtgacaa cgccaaccag
      541 gttcgatcgg gacgtgtatt ttgcagcggc ctcttcgcct tgatcgaaaa tggccatgca
      601 agccgctgcc acacccggtg aataggccaa agaaagatcg tcccgattgg caagaggctt
      661 ggtgggcacg actgaaatct ttcctgcgac cgggtaagcg tgatagtcga gcgctaattt
      721 ggtcagatca gctttggcgt ccatggtgac atttctcctg atgattgaaa taaaagtgct
      781 ggttttttac gatttttaat cgtgtagtgt atgtcggatg ggcagtgttt gtttatgtca
      841 acgtgccgct ttgtttcaac tgcctttcgc gcgtttcttg ttacgtttgt ccctgttcaa
      901 gggtatacta ctcggcatac ggcttattaa ggcgtcgtac ggttgccttc tgccttgcta
      961 tccgctaacc ggtctggccg cgaagcggaa ggttgttccc tgcatcataa cgagtgagac
     1021 actcggatag gtgaagcgca ttatgttatc ggaaatagaa cttcagtcca attcttatct
     1081 ttttggcggt aacgcccctt atgtcgaaga gctctacgag ctctacctag acaatcccgg
     1141 ttctgtccct gaccgctggc gaagctactt cgatcagttg cagcatttgc ccgcctccga
     1201 cggtctcgaa gccacccgcg atcaggcgca tgcgcccatc atcgaatcgt ttgcccaacg
     1261 cgcaagaaat aacggtttcg tggcgcgcgc gcaagagccc gatctggccg ttgccggcag
     1321 acaggttcac gtccaaacaa tcattgcggc atatcgttcg atgggttcgc gtctggccga
     1381 cctcgatccg ctcaagcgcc aagatcgtcc cgttatcccc gaactcgatc caagctccta
     1441 cggcatgctc gaatccgacc tggatcaggt ctattcggca acgaacactt atttcacgaa
     1501 agccgatacc atgacgatgc gcgacatcat caaggcattg cgcgatacat actgccgcag
     1561 cgtgggcgcc gagttcatgt acattaccga tccagctgcc aaacgctgga ttcaagagcg
     1621 cctggaatcc acgcttggcg ttccctctta ttcaaatgac cgcaagcgct atattttgcg
     1681 tcaagtcacc gaggccgaag gcctcgagcg ctttttgcac accaaatacg tcggacaaaa
     1741 gcgtttttcc cttgaagggg gtgaaagctt tatagccgcc atggacgaag tcgtaggcca
     1801 tgcgggcgac aacggcgtgc aagagctcgt ggtgggcatg gcccaccgcg gccgtctgaa
     1861 tttgctggtc aacatcatgg gcaagatgcc tgctgatttg ttcgccgaat ttgaaggaaa
     1921 acacgctgaa ggcctgaccg acggcgatgt taaataccat aacggttttt caagcgatct
     1981 ttccacgcgc ggcggcccgg tgcatttatc cctggcgttt aacccatcgc atctggaaat
     2041 cgttaacccg gttgtcgagg gcagcgtgcg cgcgcgccag gatcgccgcg gcgatgccga
     2101 gggcgttcag gtcttgcctg tgctggtgca tggcgatgcg gccttcgcgg gccagggcgt
     2161 catcatggaa accctaaacc tcgcccaaac gcgcggcttc ggtaccggcg gcaccctgca
     2221 tattgtcatt aataaccaaa tcggctttac gacgtccgat ccgcgcgata cgcgttcgac
     2281 cttgtattgc accgacgtcg tcaaaatgat cgaagcgccg gtatttcacg tcaacggcga
     2341 cgaccctgag gcggtcgtct atgttaccca gctcgccctc gattaccggc tcaagtttcg
     2401 tcacgacgtg gtggtcgata ttgtatgttt ccgcaagctg ggccacaacg agcaagacac
     2461 gccgtcgctg actcagccgc ttatgtataa aagcgtcggc aagcatcctg gcacgcgcaa
     2521 ggtttatgcg gacaaacttg tggctcaggg cgttttggcc gaaggcgagc ccgaccaaat
     2581 ggtcaaggac tatcgtcagc ttatggaaga cgggcaacgc acggtcgagc ctgtgctgac
     2641 cgattacaaa agcaagtacg caacggactg gtcttccttc ctgggcgcca aatggaccga
     2701 tcaggccgat acaggggtgc ctctggccga acttacccga atcggtgaaa ggatcaccac
     2761 catcccggaa aatttcaccg ttcatcccct ggtgcacaag cttcttgacg atcgccgaag
     2821 catgattgcc ggcgagcgcc cgcttgattg gggcatggcg gaacatctgg cttatgccac
     2881 gctgctcgcc gccggctatc ccatacgcat cacggggcaa gacgcgggcc gcggcacgtt
     2941 ttcacatcgc cacgctgtct tgcatgacca gaaacgtgaa cgttgggacg gcggcgccta
     3001 tattcccctg caaaacgttt ccgaggggca ggcttcattc acaattatcg attcagtact
     3061 gtcggaattg gccgtactgg cctttgaata cggctacgct attgcagagc ccaatgcgtt
     3121 aacgatctgg gaagcgcaat ttggcgattt cgccaatggc gcacaggtcg tcatcgatca
     3181 gttcgttacg tctggcgaag caaagtgggg acgtcattgc ggcctgacca tgatgctgcc
     3241 gcatggctac gagggccagg gccccgagca ctcttcggcc cgcatcgaac gctttttaca
     3301 actgtgcgcc gacaataata tccaagtggt gcaaccgacg aatgcgtcgc aaattttcca
     3361 tgtgttgcgt cgccagatga tccgccagtt ccgtaagccg ctgattattt ttacgcccaa
     3421 gtccttgctg cgcaacaagg gggctgcttc tcccatggcc gatttggcga acagccaatt
     3481 tttgcccgtc attggcgaaa ccgacgaaac gatcgacccg gcggccgtta cccgggtgct
     3541 ggtttgttcc ggaaaggttt attacgattc gctgcatgcg cgcgccgaag ctggccgcac
     3601 cgacgttgcg gtattgcgca tcgagcaact gtatccgttc gctcacaagt cgtttcagac
     3661 cgagttgcgc aagtatgcga acgcaaaaga ggtcatttgg gttcaggatg aaccgcaaaa
     3721 tcagggtgca tggttttaca tacagcacca tttgtacgag aacatggtgg aagggcagaa
     3781 gctgggttac gcgggccgtg cggcatcggc ttcgcccgcc gttggttatc tggccaagca
     3841 tcaggagcag caaagaaact tgcatgaaca agcgcttgcg cccaaattca aaggcgtcat
     3901 gttcaccaag taacgaatat tactaataac ggaaactata caatggctat tatcgacgtg
     3961 ttggtccctc agctttccga atcggtctcc gaggcgactt tgcttacctg gaagaagaaa
     4021 ccgggtgaag cggttcaggc tgacgagatt ctgattgaag ttgagaccga caaggtggtg
     4081 ctcgaggtgc cggcgccgaa ttctggcgtt ttggccgagg tgctaaaggg agacggcagt
     4141 accgtgctgg cgggcgaagt gctgggccgc atcgataccg aagcca
//
LOCUS       DATCXU010000163         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_221963,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000163 DATCXU010000000
VERSION     DATCXU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221963"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIR04_03630"
     CDS             <1..375
                     /locus_tag="VIR04_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY9459871.1"
                     /translation="NLSIWVGYRLRGGWGALLAVLGMVVPAMFVAIAMGAIFSQLNQS
                     KNVHLAMAGIAAAAIGLSLEMGVHAAKKAVNGILPALIVAGTFGAIFILNFNLIWVVL
                     CAAPCSIAIAYYRLRRASRPPA"
     gene            372..896
                     /locus_tag="VIR04_03635"
     CDS             372..896
                     /locus_tag="VIR04_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY9459872.1"
                     /translation="MIQTLVDLFRVFAPLSFLTVGSGQSIIPAIHRQSVVVHQWITNA
                     QFLDLFALSRLTPGPKSLLVTLVGWETAGFAGAIVASVAIFVPSALLVYTLARLWARF
                     ENSIVIKAVERGLIPVAAGMILAATGTILNAAEGGLWAWGTAIVSTLALLYTRISPFL
                     LLGLGGLLFVVIRP"
     gene            946..2337
                     /locus_tag="VIR04_03640"
     CDS             946..2337
                     /locus_tag="VIR04_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY9459873.1"
                     /translation="MLPTIVDLQKALANKTVTSAELTEAALKRIDAPDGEGRRAFTSV
                     YHAKARAAALASDTLRAAGMSRSPIDGLPISIKDLFDVANETTRAGSAVLNDAPAARR
                     NAPIVQRLIDAGAVIIGRSNMTEFAFSGLGLNPHYGTPSSPWDRTTGRIPGGSSSGAA
                     VSVADQMAVAAIGTDTGGSVRIPSAFCGLTGFKPTCGRISTQGALPLSFSLDSIGPLA
                     ASVTCCAILDAILAGQIYIAPVAPSMPHLRFAVPSSVVLDDADAHVRASFQHALNTLK
                     DAGAKIEEIELPEFEQLPHINRQGGLIGAEAWAWHRKLLADHASAYDPRVGARIMRGK
                     DISAADYIDLMQTRERWISSVEARLKPYDALLMPTVPIIAPSIKELQDSDAAYFAANG
                     LILRNPTFINFLNGCALSMPCHPAGTAPVGLMIAAPAGHDQAVLNTGLAVEQALAAKE
                     HAGRSVAPDTHRA"
     gene            2411..3241
                     /locus_tag="VIR04_03645"
     CDS             2411..3241
                     /locus_tag="VIR04_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459874.1"
                     /translation="MNSEAAASPPILRFTGVALGYGDFTVLRGIDIEVRPGQVVAMMG
                     GSGSGKTTLLRGATGQIAPQAGTISAFGENIATASGDGLRALRQRMGVLFQQGALFTD
                     LNVFENVAFPLREHRQLPEDQVVELVLEKLDAVGLRAAAHLNIGEISGGMARRVALAR
                     AIVLEPELILYDEPFAGLDPISLGITAQLIRSLTDRLNCGSVLITHDVAESFKIADLV
                     CMVGQGRLIASGSPADLSDSQDAYVHQFLNGEPDGPIAFNYPESAAFKQWLQQHKGQS
                     "
     gene            3211..4026
                     /gene="mlaE"
                     /locus_tag="VIR04_03650"
     CDS             3211..4026
                     /gene="mlaE"
                     /locus_tag="VIR04_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY9459875.1"
                     /translation="MVATTQRAILNSPAKIIVSLGHHLRTQIGSLGAFAHLLGSILAR
                     SGTAFKRPSLISQQVHFIGNYSLLIIAVSGLFVGFVLGLQGYNTLNRYGSEEALGLLV
                     ALSLTRELGPVITALLFAGRAGTSLTAEIGLMKAGEQLAAMEVMAVDPIRRVLAPRFW
                     GGVIAMPILATVFSAVGIIGGWIVGVLMIGIDPGSFWSQMQNGVDIFQDIGNGVVKSI
                     VFGIAVTLIALYEGWNAKPTPEGVSRATTRTVVSGALVVLGLDFLLTALMFSN"
     gene            4040..4552
                     /gene="mlaD"
                     /locus_tag="VIR04_03655"
     CDS             4040..4552
                     /gene="mlaD"
                     /locus_tag="VIR04_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY9459876.1"
                     /translation="MTREKTDFWVGLFVLLGFLALAFLALRAANLSSFSFAPTYQVQA
                     HFDNLGGLKIRAPVKSSGVVVGRVGAIAFDNQRFQALVTLDLDQKYKFPDDTSASILT
                     SGLLGEQYIGLTPGGDDKMLAQNSQIKYTQSAVVLEELISKFLYSSASKQGGAATAEG
                     DTKAPAPPKP"
BASE COUNT          901 a         1408 c         1334 g         1016 t
ORIGIN      
        1 aatttgtcga tctgggtggg ttaccgtctg cgcggcggct ggggcgcgct gcttgcagtg
       61 ctggggatgg tggtgccggc catgttcgtg gccattgcga tgggtgcgat cttctcgcag
      121 ttgaatcaat cgaaaaatgt ccatcttgcc atggccggca tcgctgcggc ggccattggg
      181 ctctctctgg aaatgggcgt ccacgcggcg aagaaggcgg ttaatggaat actgcccgcg
      241 ctcatcgtgg cgggcacgtt cggcgccatc ttcattttga acttcaattt gatttgggtg
      301 gtgctatgcg ccgccccctg ttccattgcc atcgcctatt accggctgcg ccgcgcgtca
      361 aggccgccgg catgatccag acgcttgttg atttattcag ggtattcgcg cccctttcgt
      421 ttctgacggt cggaagcggt caaagcatta ttccagccat tcatcgccaa tcggtggttg
      481 tgcatcagtg gatcaccaat gcgcaatttc tggatttgtt cgcgctatca cgcctgacgc
      541 caggccccaa atcattgctg gtcacattgg tcggctggga gacagccggc tttgccggag
      601 cgatcgtcgc ttcggtggcc attttcgtgc cttccgcgct attggtctat acgctggcga
      661 ggctttgggc ccgcttcgaa aacagtattg tcatcaaggc cgtcgaacgc ggccttatac
      721 ccgttgcagc aggcatgatt ctggccgcca cgggaacaat cctgaacgcg gccgaaggcg
      781 ggctatgggc atggggaacc gccattgtct cgaccctggc gttactctat acccgcatca
      841 gccccttttt gctgttagga ttgggtggtt tgttatttgt cgtcatccgg ccgtgatcga
      901 tgtccgcgat ccggcctgtc ctggaaccct tgccggatac ctgccatgct gcccactatc
      961 gtcgatctgc aaaaagccct ggccaacaaa acggtcacct ccgctgaatt aaccgaagcc
     1021 gcgctgaagc gcattgacgc gcccgacggc gaaggccgcc gtgcattcac ttcggtttac
     1081 catgccaaag cgcgcgccgc cgcgctcgcg tccgataccc tgcgagccgc cggcatgagc
     1141 cgatcgccga ttgacgggct gcctatttca ataaaggatc tgttcgacgt cgccaacgaa
     1201 acgacacgtg ccgggtcggc ggtgcttaac gacgcccccg ccgcacgacg caatgcgccc
     1261 atcgttcagc gcttgatcga tgcgggcgca gttattatcg gccgcagcaa tatgaccgaa
     1321 tttgctttct cgggcctggg tttaaaccct cactacggaa cgccaagcag cccatgggac
     1381 agaacgacgg gccgcattcc gggggggtcc tcatccggcg ccgccgtatc cgtggccgat
     1441 caaatggccg tcgcggccat aggcaccgat accggcggct cggtgcgcat tccgtcagcg
     1501 ttttgcggtt tgacgggctt taaacccact tgcggccgca tttcaacgca aggcgcgctg
     1561 ccgttgtcat tcagtctgga ttcaattggg ccactggccg catcggtcac gtgctgcgcc
     1621 attctcgacg ccattctggc ggggcaaatc tatatcgccc ccgtcgcccc gtcaatgccg
     1681 catctgcgat ttgccgtgcc gtcctcggtc gtgctcgacg atgcggacgc acacgtgcgc
     1741 gcatcctttc aacatgcact gaatacgctc aaagacgcgg gcgccaaaat cgaggaaatt
     1801 gaattaccgg agttcgagca gttgccacac atcaaccgcc aaggcggcct tatcggagcc
     1861 gaggcctggg cctggcaccg caagctgctt gctgatcacg ccagcgccta cgacccgcgg
     1921 gtgggagccc ggatcatgcg cggcaaagac atcagcgccg ccgattacat cgacctgatg
     1981 caaacgcgcg agcggtggat ttcttcggtc gaagcgcgtc ttaagcccta tgacgcgcta
     2041 cttatgccaa ccgtgccaat tatcgcaccc tctatcaaag aactacagga ctccgatgcc
     2101 gcttattttg cggccaacgg tctgattttg cgcaacccca cgttcatcaa tttccttaat
     2161 gggtgcgcgc tatcgatgcc ttgccacccg gccggaaccg cgccggtggg cttgatgatc
     2221 gccgccccgg ccggccacga tcaggccgtt ctgaacaccg gtttggcggt ggaacaggcg
     2281 ctcgccgcca aagaacatgc cggacggtct gttgcaccgg atacccatag ggcatgaaat
     2341 acaatcgctg agtttacgca cccacgcaaa tctgttacac ctgttttgtt caacctttgt
     2401 catttgaccc atgaattctg aagcagccgc gtccccgccg atccttcgct ttaccggcgt
     2461 cgccttgggc tatggggact tcacggtgtt gcgcggcatc gatatcgaag tgcgcccagg
     2521 ccaggtggtc gccatgatgg gcggctcggg atccggcaaa accacccttc ttcgcggcgc
     2581 gaccggccag attgccccgc aggccggcac aattagcgca tttggcgaaa acatcgcgac
     2641 cgcctcgggc gacggcttgc gcgcgttgcg ccaacgaatg ggggttctgt ttcagcaggg
     2701 cgctcttttc actgatctga atgtattcga aaatgttgcg tttccgctgc gcgaacacag
     2761 gcaactaccc gaagaccagg tcgttgaact ggtgctggaa aagctggatg ccgtcggatt
     2821 gcgcgcggcc gcccatttga acattgggga aatctcgggc ggcatggctc gccgcgttgc
     2881 tctggcacgc gccatcgtgc ttgaacctga acttattctt tatgacgagc cttttgccgg
     2941 cctggacccc atttccctgg gcattacggc gcagttgatt cgcagcctga cagaccggct
     3001 gaactgcggc tcggtgctga tcacgcacga cgtcgccgaa tcgttcaaga ttgcggacct
     3061 tgtctgcatg gtcggccagg gacggcttat tgcgagcggc tcgccggcgg atctgtctga
     3121 ttcgcaagac gcctacgtgc atcaattcct gaacggcgaa cccgacgggc cgattgcctt
     3181 caattacccg gaatcggctg ccttcaaaca atggttgcaa caacacaaag ggcaatcttg
     3241 aattccccgg caaaaatcat cgtgtcgcta ggccatcact tgcgcaccca aataggcagc
     3301 ctgggcgcat tcgcgcacct cttgggcagc atactggcgc gcagcggcac cgcattcaag
     3361 cgccccagcc tcatctcgca gcaagtccat ttcatcggca actattcgct gctgattatc
     3421 gcggtatcgg ggctattcgt gggcttcgtt ttaggcttgc agggctacaa cacgctgaat
     3481 cgttacggct ccgaagaagc gctggggctg ctggtggcgt tatcgctaac gcgtgaactg
     3541 ggccccgtca tcacggcgct gctgtttgcg gggcgggcag gcacctctct gaccgccgag
     3601 atcggcctga tgaaggccgg cgaacagctc gccgccatgg aagtcatggc ggtcgatccc
     3661 atccgccgcg tactggcgcc gcgtttctgg ggcggcgtta tcgccatgcc gattctggcc
     3721 accgtttttt ccgcggtggg cattatcgga ggctggatag tgggcgtgct catgatcggc
     3781 atcgacccgg ggtcgttctg gtcgcaaatg caaaatggcg tcgatatttt ccaggatatc
     3841 ggcaatggcg tcgtcaagag cattgtcttc ggaatcgccg tcactctgat tgcgctctac
     3901 gaaggctgga atgccaagcc cacgcccgaa ggggtttccc gcgccaccac acgcaccgtg
     3961 gtcagtggcg cacttgttgt attggggctg gatttcttgc tgacagccct gatgttcagt
     4021 aactaaagac ggaatcatca tgacgcgcga aaaaaccgat ttctgggttg ggctatttgt
     4081 tcttttaggc tttctggccc tggcatttct ggcgctgcga gccgccaacc tgagctcgtt
     4141 ctcgttcgcg cccacctatc aggttcaggc gcatttcgac aatctgggcg gccttaaaat
     4201 tcgggcgccg gtaaaaagca gcggggtggt agtgggacgc gttggcgcca tcgccttcga
     4261 taatcagcgc ttccaggcgc tggtcacgct ggatctggac caaaaataca aattccccga
     4321 cgacacgtca gcctctattc tgacctccgg cctgctgggc gagcagtaca ttggcctgac
     4381 gccggggggc gacgacaaaa tgctggctca gaatagccag atcaaataca cccaaagtgc
     4441 cgttgttctt gaagagctca tcagcaaatt tctgtatagt tcagccagta aacaaggcgg
     4501 ggccgcaaca gcggaaggcg acacaaaagc gcccgcgccg ccaaaaccat aaaatagcct
     4561 gatcgaagac aaagaaagcc aaaaagacac caatgatttc ccactatgcc actgcggtaa
     4621 accggtcgaa acggctggcg aaacttgccc caatcctgg
//
LOCUS       DATCXU010000164         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_264375,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000164 DATCXU010000000
VERSION     DATCXU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264375"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VIR04_03660"
     CDS             <1..539
                     /locus_tag="VIR04_03660"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY9459877.1"
                     /translation="SGVLRHYRRGGFMARLSLDRYFWVGARRTRSFAEFELLDFMSRR
                     GLAVPIPLAAIYWRHAASYRAAILVERISNVQALARALTRPHHGAVARAIFAMHNAGV
                     WHADLNAYNILLDGRNQAWLIDFDRGRRRAITPRLRDANLLRLRRSLIKVAGTAGLDY
                     WNELNRAYQDLLRDTGDR"
     gene            605..1705
                     /locus_tag="VIR04_03665"
     CDS             605..1705
                     /locus_tag="VIR04_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9459878.1"
                     /translation="MKINLAGRLAIGMALVLAAAPLWAQDRVVNVYNWAEYTAPDTIS
                     GFEKATGIKARYDVYDSNDTLQAKLLTGKSGYDVVVPSTNYAARQIKGGLFQKLDKSK
                     MPNLKYLDPDIMALVAIIDPGNDYLVPWGYGTNGLGYNVTKVREIMGPDAPLGSWDML
                     FKPENAQKLKACGISILDEAAQVFPAVLHYLGKDPNSSQPDDYKLALDLLKKIRPYVR
                     QFSSSGYIDELASGGLCMVYGYSGDVMIARYRAGQAKKPYTIDYFIPQGGSPAWFDTM
                     AIPKDAPHVAEALAFINYIETPQVHAAITNTMFYPNANKEARKYVLKEVADNPMIYPP
                     PDVAKTLYVIKPQPLAIQRLQTRMWAELKSGR"
     gene            1709..2839
                     /gene="potA"
                     /locus_tag="VIR04_03670"
     CDS             1709..2839
                     /gene="potA"
                     /locus_tag="VIR04_03670"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEY9459879.1"
                     /translation="MDGGYPGTQAGSDPSDGFVRLEQVVKIFDGTTAVQSVDLTVRRH
                     EVFALLGSSGCGKTTLLRMLAGFERVTSGRIYLDDEDIPGLPPYRRPVNMMFQSYALF
                     PHMTVEANVAFGLKQEGVPKNEIRERVFETLDLVQMAAFSRRKPYQLSGGQQQRVALA
                     RSLIKQPKLLLLDEPMSALDKQIRQKTQIELSRILEQVGVTCIMVTHDQEEAMTMAAR
                     LAFMSEGRIVQCGTPREVYLCPNSRFVAGFIGSTNLFTATVVVDESDHALIESSELTH
                     ALHVGHGISESLGMQVHVSIRPEEIRLSRSAPQRQSNWERGIVDHVAWMGSYARYRIR
                     LDSGFILEANVPSLILAQADAPAVDEEVFVAWSSDSVTVLPS"
     gene            2836..>3467
                     /locus_tag="VIR04_03675"
     CDS             2836..>3467
                     /locus_tag="VIR04_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEY9459880.1"
                     /translation="MRKLARRRLMPSARALTIGPPFIWLGLFLLVPFLLVLKISFADL
                     RFGVPPYTALTELKDHTLTLAVHLRAYALLFSDSLYIATYLNSIKMAAIATLGCALIG
                     YPLAYYIARSRPAVRSGLLLAVILPFWTSLLLRVYAWVGILRNDGLLNRLLEWLGVIN
                     APFEIYRTDLAVYIGLIYAYLPFFVLPLYANLVKLDLRLVEAACDLGARP"
BASE COUNT          708 a          909 c         1004 g          846 t
ORIGIN      
        1 gctccggcgt gctgcggcac tatcgccgcg gagggttcat ggcccggctc agtctggacc
       61 gctatttctg ggttggcgcg cggcgcacac gctcgtttgc tgaattcgag ctactggatt
      121 ttatgagccg tcgcgggctg gcagttccca taccactggc agctatttac tggcggcatg
      181 cggcgagcta tcgggcagct atcctggtcg agcgcatttc gaatgtgcag gcgctggcca
      241 gagcactaac ccgcccgcat cacggcgctg tggcgcgcgc catttttgca atgcataatg
      301 ccggcgtctg gcatgccgat ttaaacgcct acaacatttt gctggacggg cgaaatcaag
      361 catggctaat tgactttgat cgtgggcgca ggcgggcgat aaccccacgc ctaagggatg
      421 ccaatttgtt gcgcttgcgt cgctcgctga tcaaggtggc ggggacggcg gggcttgact
      481 attggaacga gctgaaccgc gcctatcagg atttactgcg cgacacgggt gaccgataag
      541 gtggaaagct ttattatcac gcggtttgag ttttggattt ccttgagtta cgggggtgct
      601 tgcaatgaaa atcaacttgg ccgggcgatt ggcgattgga atggcgctgg tgttggcggc
      661 ggcgccgctg tgggctcagg atagggttgt gaacgtctat aactgggctg aatacacggc
      721 gcccgatacg atctcgggct ttgagaaagc taccgggatc aaagcgcgtt acgacgtata
      781 cgacagcaat gacactttgc aagcgaagtt gttgaccggc aagtcgggtt acgacgtcgt
      841 agtgccatca accaattatg cggcgcgcca gattaaaggg ggcttgttcc agaagctcga
      901 caaatcaaaa atgcccaact tgaaatacct ggaccccgat atcatggccc tggtcgccat
      961 tatcgaccca ggcaatgatt atcttgttcc gtgggggtat ggcaccaatg gtttgggcta
     1021 taacgtcacc aaggttcgcg aaatcatggg accggacgcg ccactgggca gttgggacat
     1081 gttgtttaaa cccgaaaacg cacaaaagtt gaaagcgtgc ggcatttcaa tacttgacga
     1141 ggccgcgcaa gttttcccgg ccgtgttgca ttatcttgga aaagatccca atagcagtca
     1201 gccggatgat tacaaattgg cgcttgattt gctcaagaaa attcgacctt atgtcaggca
     1261 attcagttcg tcgggctata tcgacgagct tgccagtggc ggcctgtgca tggtttatgg
     1321 ctattccggc gatgtcatga ttgcgcgtta tcgcgccggc caggcgaaaa aaccctatac
     1381 catcgattat tttattccgc agggcgggtc gccggcctgg ttcgacacga tggcgattcc
     1441 gaaagacgcc ccgcatgtgg ccgaggcgct ggcgttcatc aactatatcg aaaccccgca
     1501 agtgcacgcc gccatcacca atacgatgtt ttatcccaac gccaacaaag aagcgcgcaa
     1561 atacgttctg aaagaggttg ccgacaaccc catgatttat ccgcctcccg atgtggcgaa
     1621 aaccttatac gtgatcaagc cacagccgct tgccatccag cgattgcaga cgaggatgtg
     1681 ggccgaactg aagtcggggc ggtaggtcat ggacggcggc tatcctggca cgcaggccgg
     1741 gtctgacccg tcagatggat tcgttcgcct cgaacaggtg gtaaagatct ttgacggtac
     1801 gacggccgtt cagtcagttg atttgactgt acgccgccac gaagttttcg ccttgcttgg
     1861 cagttcgggt tgcggcaaga caaccctgct gcgcatgctg gcgggtttcg agcgagtcac
     1921 ctcgggccgc atttatctgg acgacgaaga cattcccggc ttgccgccgt atcgccggcc
     1981 ggtcaacatg atgtttcagt cttatgcctt gtttccccac atgacggttg aagccaatgt
     2041 cgcgttcggg ctaaaacaag agggtgtccc caaaaacgag attcgcgaac gtgtttttga
     2101 aacactcgat ctcgttcaga tggctgcatt ttcccggcgc aagccctacc aattgtccgg
     2161 tggacagcag cagcgtgttg cgctggcgcg cagcctcatc aagcagccca agcttttgtt
     2221 gctcgacgag cccatgtcgg cgcttgacaa gcaaatacgc caaaaaactc agatcgaact
     2281 ctccagaatt ctggagcagg tgggcgtgac gtgcattatg gtgacgcacg atcaggaaga
     2341 ggcgatgacc atggcggcgc gactggcttt catgtccgaa ggcagaatcg tgcaatgcgg
     2401 aacgccccgc gaggtctatt tgtgtccgaa ttcgcgtttc gtcgcgggat ttataggctc
     2461 gaccaacttg tttacggcta cggttgtagt cgacgaatcc gaccacgcgc tcattgaaag
     2521 ctcggaatta actcacgccc tgcatgtcgg ccatggcatc agcgaatcgc tgggaatgca
     2581 ggtgcatgtg tcgatacggc cggaggagat ccgtttgtca cggtcagctc cccaacgtca
     2641 gtcgaattgg gagcgcggca tagtcgatca cgtggcctgg atgggcagtt atgcgcgcta
     2701 tcgtattcgc ctggactcgg gctttattct cgaggcgaat gttcccagcc tgatcctggc
     2761 tcaggctgac gcgcccgcag tggacgaaga ggtgtttgtc gcgtggtcca gcgacagcgt
     2821 tacggttttg ccatcatgag gaagcttgcg cgccgccgcc tgatgccttc ggcacgagcg
     2881 ttgacgatag gcccgccatt catctggctt ggcctttttc tgttagtgcc ttttttactg
     2941 gttttaaaaa tcagtttcgc cgatcttcgt tttggcgtcc ctccctacac agccttgacc
     3001 gagcttaaag accataccct gacgctggcc gtgcatttgc gcgcgtatgc gttgttattt
     3061 agcgatagtc tctatatcgc gacctatttg aattcaatca agatggcggc gattgcgacg
     3121 ctggggtgcg ctctgattgg atatccgctg gcttactaca tagctcgatc gcgcccggcc
     3181 gtgcgcagtg gtttgctgct tgccgtcata ctgccatttt ggacgtcgct gttattgcgc
     3241 gtttatgcgt gggttgggat tttgcgcaac gatggattat tgaacaggct gcttgaatgg
     3301 cttggagtga tcaatgcgcc gtttgaaatt tatcgcaccg atttggctgt ttatatcgga
     3361 ctcatttacg cctatctgcc cttttttgtt ctccccttgt atgcgaattt ggtcaagctc
     3421 gatttacggc tggttgaggc ggcctgcgat ctgggcgcgc gtccctg
//
LOCUS       DATCXU010000165         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_331486,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000165 DATCXU010000000
VERSION     DATCXU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331486"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VIR04_03680"
     CDS             <1..240
                     /locus_tag="VIR04_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY9459881.1"
                     /translation="PARDRIIADLTRRYLDMMLRYPGLDHVMCWGMLDHHSWLQNSTR
                     RPDGMLKRGCPYDPDYQPKPMRQAIADAFRNAPKR"
     gene            343..591
                     /locus_tag="VIR04_03685"
                     /pseudo
     CDS             343..591
                     /locus_tag="VIR04_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(612..>3699)
                     /locus_tag="VIR04_03690"
     CDS             complement(612..>3699)
                     /locus_tag="VIR04_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY9459882.1"
                     /translation="ALHASAAVAGELALSLPEQPLSDSLRGVAQKTGRNILFTPEAVS
                     QLRAPAIEGKYSAEAAVALLIKNTSLEVIGDGANGLIVRLRPQAKPELPATESAPPLT
                     ETVVVTGSRLIFSATDSPTPVESFPAKQLRATTPTDVPDALNKLPVFQGSTTTRGTNN
                     ASFNAAGNVLNLRNFGSQRTLVLLDGHRIAPSNANGTVDIDALPQMLIQRVEVVTGGA
                     SAVYGSGAVTGVVNFILNKNFNGLRIEANSGLSTYGDAASYNIGLATGSALFGGKGHI
                     ELALRRFYQDGVLHNDRPEGPLYWLLTGSGTPAKPFTSTSNARLTRFGGLISCAGCIA
                     DGQRFNGTNSIGPYNVGIPTGTSGVSQGGDGGYSSLAQITSALGTSEAFGHFSYDLSD
                     NTTLYLQASGAESYTKGSFQNHAFQTGAVPNTFLKTNPYMPASLAPLFAGTSAAFSVT
                     GYFDMGYPRSGYQSNGLQRNLQVATGLDGKLADGHFPWNIYYTHGEARLHEDDPTNIN
                     YQRMYAAIDGVAGNTPGSATCWVTTTANADLYPGCIPLNPFGADAIGMSAITPAMDRY
                     ISGDTGFWLTNIMDNVGAGISGDMLQLPAGPLKLALSGEARWQSYEVRSNASPTATVD
                     CTGLRTSVPGAPDYALCNPLTPLWQNNVVASLAPVRESVWEIAGEANIPLAKNLPFFE
                     RITTDIAGRYTAYGTSGVVQTWKIGFDWHLSDSIVLRAANSIDIRAPTLADLFAPAGQ
                     GIQSFSDTLHTGVTGQNFAVSRSNPNLTPEVARTYTAGVTLTPMANLSLSADYYTINL
                     KNAISGVNGGSNTVLRICEDSGGASPLCSLVVRPLAFSDRSAANFPTQIISEGKNTAY
                     NKLEGIDLELTYMLPLRTVEADWPGTLSLRALANYQPVNQSIQYPGAPLVFTSYPKAR
                     LSLFAAYTVGPWSINLLDRWISGFAKGTQIGQVYADPRVPATNYVDINIDRAFPIDDG
                     SLNAYLSVQNVFDQRPRVNPTNSTNPGLYFMGVQGSTTSLYDAIGRYFTIGLHVDL"
BASE COUNT          733 a         1039 c         1149 g          778 t
ORIGIN      
        1 ccagcccgcg accggatcat cgcggatctg acccggcgtt atctcgacat gatgctgcgc
       61 tatcccggcc tggatcatgt gatgtgctgg ggcatgctgg accatcattc ctggttacag
      121 aacagcaccc gccgccccga cggcatgctg aagcggggtt gtccctacga tcccgactac
      181 cagcccaagc ccatgcgcca ggccatcgct gatgcgttcc gcaatgcccc gaagcgctga
      241 aagcgtgcgg ggcgatcagt aagcggccgt ggccttggct gtccaagtcg gaaagctccc
      301 catggccctc tcagaatatc cgcttttggg cgcaaagcgg acaacatcaa ttacgacaag
      361 ttcaacatcc agcgcggcga tacgctaacc cagccgcatt ttcagggcga caaacccttt
      421 gatgccatcg tccccaatcc gccctattct gtaaaatgga tcggctcgga tgatccaaca
      481 ctgatcaatg atgaccgctt tgccccggtg ggcgtactcg ccccgaaatc aaaggccgac
      541 tttgcctttg tgccccatgc gccaatttat ctgtccgcca aaggccgcgc ggggcggggg
      601 ctcagtggtt gctacagatc aacgtgaaga ccgatggtga agtaccgccc gatggcgtcg
      661 taaaggcttg tggtgcttcc ctgcacgccc atgaagtaga ggccagggtt ggtcgagttg
      721 gttggattca cgcgcgggcg ctggtcgaaa acattttgca cagacagata cgcattcaga
      781 cttccatcat cgatcgggaa tgcacgatcg atgttgatgt cgacatagtt tgtcgcggga
      841 acgcggggat cggcatagac ctgacctatt tgtgtgccct tagcaaatcc gctgatccat
      901 ctgtcgagca gatttatgga ccagggtccg accgtgtatg cagcgaagag gctgaggcgg
      961 gccttgggat aagacgtaaa gactagtggc gcgcctgggt actgaatcga ctggttgacg
     1021 ggctgataat ttgccagcgc ccgtaaactc aatgtgcccg gccagtcagc ctcaaccgtg
     1081 cgcagaggca gcatgtaggt cagctcaagg tcaatgcctt ccagcttgtt gtaagccgta
     1141 ttcttgcctt cggaaattat ctgggtgggg aagttggctg cgctccggtc gctgaacgcc
     1201 agagggcgca cgacgaggct gcaaagaggg gacgcgccgc cggagtcttc gcagatccta
     1261 agcacggtgt tgctgccgcc attcacgcca ctgatcgcgt tcttcaggtt gatagtgtag
     1321 tagtccgcgg aaagcgaaag gttcgccatg ggtgtcaggg tgacgccggc ggtataggtg
     1381 cgcgccactt ccggcgtgag gttggggttg ctccgtgaga ccgcaaagtt ctgaccggtg
     1441 acacctgtgt gcaatgtgtc gctgaagctc tgtatgccct ggccagcagg ggcgaagagg
     1501 tcggccagcg tcggggcgcg aatgtcgatg gagttcgccg cgcgcagaac gatgctgtcg
     1561 gaaagatgcc agtcaaatcc gattttccag gtctggacca cgcctgaagt gccataggcc
     1621 gtatagcggc cagctatatc ggtcgttata cgctcgaaaa atggcaggtt ctttgccagt
     1681 ggaatattcg cttcgccggc gatttcccag acgctctccc gtacgggcgc cagggaggcc
     1741 accacattgt tctgccacaa cggcgtcagg gggttgcaga gcgcataatc gggcgccccg
     1801 ggtacactgg tgcgcagccc cgtgcaatcg accgtggcgg tcggggaggc gtttgagcgc
     1861 acctcatatg attgccagcg cgcctcgccg ctgagagcga gctttagggg gcccgccggc
     1921 aattgcagca tgtcgccgga gatgccggca ccaacattgt ccatgatatt cgtcagccag
     1981 aaccccgtat cgcccgatat gtatctgtcc atggccggcg ttatggcgct cattccgatg
     2041 gcgtctgcgc caaacggatt gaggggaatg cacccggggt aaagatctgc gttggcggtg
     2101 gttgtcaccc agcaggtggc agagcccggc gtattgcctg caaccccgtc tatcgctgcg
     2161 tacattctct ggtagtttat attggtcggg tcgtcttcgt gcagccgtgc ttcgccgtgg
     2221 gtgtagtaga tattccaggg aaaatgtcca tctgccagct tgccgtccag gcccgtggcg
     2281 acctgaaggt tgcgctgcaa tccgttggat tggtaaccgg aacgcggata gcccatgtca
     2341 aagtagccgg ttacgctgaa tgccgccgat gtcccggcaa aaagcggtgc cagcgacgcg
     2401 ggcatgtagg gattggtttt caggaacgtg tttggcactg cgccggtctg gaaggcgtgg
     2461 ttctggaagc tccccttggt gtaggattcc gcgcccgaag cctgcaggta cagggttgtg
     2521 ttgtcgctga gatcatagct gaagtggcca aaggcttcgc tggtccccaa tgcggaagtg
     2581 atctgtgcaa gagacgagta accgccgtcg ccgccctggc tgacgccgct ggttccggtg
     2641 ggaatgccta cattgtaagg gccaatgcta tttgtgccat tgaagcgctg gccgtcggcg
     2701 atgcagccgg cacaactgat caggccaccg aagcgagtca atcgtgcatt ggaagtgctc
     2761 gtgaaaggct tggcgggcgt tcccgagcct gtaaggagcc aatacaaggg gccttccggc
     2821 cggtcattgt gcagaacacc gtcttggtag aagcgcctta gtgcaagctc gatatgtcct
     2881 tttccgccaa agagggctga accggtcgca aggccgatat tgtagctggc ggcatcgcca
     2941 taggtcgaaa gaccggaatt ggcttcgatc cgcaacccgt tgaaattctt gttcaggata
     3001 aaattgacca ctccggtaac ggcgccggag ccataaaccg ccgaggcgcc tcccgttacg
     3061 acctcgacac gctggatgag catctggggc agggcgtcaa tatcgactgt accattggcg
     3121 ttcgatggcg ctatgcgatg gccatccagc agcaccaggg tgcgttgcga tccgaaattg
     3181 cgcaagttca acacattgcc ggcggcgtta aagctcgcgt tgtttgtccc gcgggtggtc
     3241 gtcgatccct ggaagaccgg caacttgttg agggcatccg gaacgtcggt aggcgtggtt
     3301 gcgcgcaatt gcttggcagg aaagctctcc acaggcgtcg gcgaatccgt ggcgctgaaa
     3361 atcagccgtg agcctgtcac gacaaccgtt tcggtcaggg gaggggcgga ttctgtagct
     3421 ggaagttctg gttttgcttg cggccggagc cgcacaatca atccatttgc gccgtcgccg
     3481 atgacctcga ggctcgtgtt cttgatcaga agtgctactg cagcttctgc gctgtacttg
     3541 ccttcgatgg cgggagctcg caactgcgat accgcctccg gcgtgaaaag aatgttgcgg
     3601 cccgtcttct gcgcaacacc gcgcagagaa tccgagagcg gctgctcggg caacgaaagg
     3661 gccagctctc ccgcaaccgc cgcgctcgca tgcaacgcc
//
LOCUS       DATCXU010000166         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_249889,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000166 DATCXU010000000
VERSION     DATCXU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249889"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2007)
                     /gene="acnA"
                     /locus_tag="VIR04_03695"
     CDS             complement(<1..>2007)
                     /gene="acnA"
                     /locus_tag="VIR04_03695"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY9459883.1"
                     /translation="RALAGWNAAAAPDREIAFTPARVILQDFTGVPAVVDLAAMRDAM
                     VELGGDPGLINPSAPVELVIDHSVIVDTFGKNDAFKRNMEREYERNVERYQFLRWGQD
                     AFKDFRVVPPDTGIVHQVNLEHLSRVVFTRDDDGMKAAYPDTCIGTDSHTPMVNGLGV
                     VAWGVGGIEAEAAMLGQPISMLIPQVVGVKLTGHLPEGTTATDLVLTITEMLRQHHVV
                     GKFVEFYGPGVAQVPLANRATIGNMSPEYGSTISIFPIDDETLRYLRLTGRSDEQIAL
                     VEAYAKAQGLWHDPDVELRYSQRLELDLSSVVPSIAGPKRPQDRISLKSAKEAFHGAL
                     ATILSGSAPKTRGYDKAVAESFPASDVPAFDDSARPSAAPTNSVDKAPAGDGRQHDPV
                     TITLDDGVKCTVDHGSVVIAAITSCTNTSNPMVMIAAALLAKKAVEKGLTRKPWVKTS
                     LAPGSRVVMDYYERAGLMKYLEALGFYLVGFGCTTCIGNSGPLIPQVSKAVNDHDLSV
                     VSVLSGNRNFEGRIHGEIKMNYLMSPPLVVAYALAGTMDTNLFKDPLGKGSDGKDVFL
                     KDIWPSTAEVQAVVESSIASDMFKTGYSDVFTGNEAWRALPTPEGDIFDWDATSTYVR
                     KPPYFENMPHKPDPVTNITGARVLALLGDSVTTDHISPAGSIAKD"
BASE COUNT          433 a          586 c          568 g          420 t
ORIGIN      
        1 gtccttggcg atggagccgg ccgggctgat gtggtcggta gtaaccgagt cgcctaacaa
       61 ggccagcaca cgcgctccgg taatgttggt tacgggatcg ggtttgtgcg gcatgttttc
      121 aaagtaaggc ggtttgcgca cgtaagtgga agtggcgtcc caatcaaaaa tgtcgccttc
      181 cggcgtgggt agcgcgcgcc aggcttcgtt gccggtgaac acatcggaat agcctgtttt
      241 gaacatgtcg gaggcgattg acgattccac tacggcctgc acttcagcgg tcgacggcca
      301 gatgtctttc agaaaaacgt ccttcccgtc gctgcctttg cccagcggat ccttaaacag
      361 gttggtatcc atcgtgccgg ccaacgcgta agcaaccacc agcggcggcg acatcagata
      421 attcatcttg atttcgccgt ggatgcggcc ttcgaaattg cgattgccag aaagcacaga
      481 tactacagac aaatcatgat cgttcaccgc cttgctgacc tgcggaatta gcggacccga
      541 attgccgata cacgttgtac agccaaagcc caccagataa aagcctagcg cttccaggta
      601 cttcattagc ccggcgcgtt cgtaatagtc catcaccact ctggagccgg gagcaagact
      661 ggtcttgacc catggcttgc gggtcaatcc cttttcgact gcttttttgg ccaacaacgc
      721 ggcggcgatc atcaccatcg ggttggaagt attagtgcat gacgtaatgg cggcaatcac
      781 aacagacccg tgatccacgg tgcatttgac gccatcgtcc agggtaatgg tgaccggatc
      841 gtgctgcctt ccgtctcctg cgggggcctt gtccacgctg ttcgtgggtg cggcggaagg
      901 tcgggctgaa tcatcgaacg ccggcacgtc ggacgccggg aatgattcag ccacagcttt
      961 gtcgtaaccg cgcgttttgg gcgccgaccc gctgagaatg gtggccaatg caccatgaaa
     1021 cgcctctttg gcgctcttga gagagatacg gtcctgcggg cgcttgggcc ccgcaatgga
     1081 cggcaccacg gacgacagat caagctcgag ccgttgggaa taacgcagct cgacatcggg
     1141 atcatgccac aaaccttgtg ccttggcgta ggcctcgacc agcgcaatct gttcgtccga
     1201 acggccggtt agccttaaat agcgcaatgt ttcatcgtcg atggggaaga tcgaaatcgt
     1261 cgaaccatat tcagggctca tgttgccgat agtggcccga ttggccaaag gcacctgtgc
     1321 aacgccagga ccataaaatt caacgaattt gcccaccacg tggtgctgcc gcagcatctc
     1381 ggtaatagtc agcaccagat cggtggctgt ggtgccttcc ggcaaatgac cggtgagctt
     1441 gacgcccacg acctgcggaa tcagcatgga aatcggctgg cccaacatgg cggcttcggc
     1501 ttcaattccg ccaacgcccc aggcaaccac gcccagccca ttgaccatcg gcgtgtgcga
     1561 atcggttccg atacaggtgt cgggatacgc cgccttcatg ccatcgtcgt cgcgcgtaaa
     1621 aactacgcgg gacaaatgtt ccagattcac ctgatgcaca atgcccgtat cgggcggcac
     1681 cacccggaaa tctttgaagg cgtcctggcc ccaacgcaga aactggtagc gttctacatt
     1741 gcgctcgtat tccctttcca tattgcgttt aaatgcgtca ttcttgccaa acgtgtccac
     1801 aataaccgag tggtcgatga cgagttcgac aggcgccgat ggattgatca ggcctggatc
     1861 gccgcccagc tcgaccattg catcgcgcat agcggccaga tcaaccacgg cgggaacgcc
     1921 cgtgaagtcc tgcaaaatca cgcgcgccgg cgtaaatgcg atttcgcgat ctggcgccgc
     1981 agcggcgttc cagccagcga gcgcgcg
//
LOCUS       DATCXU010000167         9571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_631434,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000167 DATCXU010000000
VERSION     DATCXU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631434"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VIR04_03700"
     CDS             complement(<1..1400)
                     /locus_tag="VIR04_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY9459884.1"
                     /translation="MNQTSPTSTSGSGSAGADQLPYVGRPMVRVEDAALLTGRGAYAD
                     DVGVKPGTLYAAILRSPYGHAEILSIDAAAALDSAGVRTVLTGVDVKKWSVPFVVGVK
                     QPMEHWCLAMERVRYVGEPVAVVVAESRYLAEDALDLIEVEYKQLPVAIDSEEATHPD
                     VPVLHPAVGSNVISDRHFCYGDPDAAFSAAKHRVSLKVRYPRNSTTPIECFVVVAEYL
                     AGNDGYDVLSNFQGPFALHPVVALALKVSGNRLRLRFVRDSGGSFGVKQAVSTYVTLL
                     CLAARKAGAPVKWVEDRLEHLQASASSPNRVTTIEAAVQPDGEITALRYDQLDDCGAY
                     LRAPEPATLYRMHGILTGAYKVSNLEVHNRVVLTNKTPSALVRGFGGPQMYFALERLI
                     QRIAVELNLDPLDVIRRNLINANAFPYRAPAGALIGSGDYQKAISLGQERGGLAELVA
                     RRAEARSQGRLYGIGYA"
     gene            complement(1523..2092)
                     /locus_tag="VIR04_03705"
     CDS             complement(1523..2092)
                     /locus_tag="VIR04_03705"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY9459885.1"
                     /translation="MADPKRLIVGISGASGIVLGVRLLEALAETEIETHLVMTRAAKV
                     TLAHELALKVSDIERLADKVYSIDDIGAAISSGSFKTMGMVVAPCSIRSLSEISTGVT
                     SNLLTRAADVILKERRRLVLLVRETPLHLGHLRSMTQVTEMGAVVMPPVPAFYARPTT
                     VADVVNHTVGRVLDLFEIETGLVKRWGES"
     gene            complement(2192..2758)
                     /locus_tag="VIR04_03710"
     CDS             complement(2192..2758)
                     /locus_tag="VIR04_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEY9459886.1"
                     /translation="MKKREKAASGSNQQYAEDYWAFSLAVYDYPEVAQACLILQNSAG
                     VDVNVMLIALWAANRLGRAITREEISAGDREIAVWREEVVEPLRKIRTRLKEGFGGAH
                     LDTDGLRNLVKSAELNAERVEQGMLAGWVSALPPGDEPLNLAGCTRTVGSVIDFYANK
                     MNSVACMADLRHLSDKIAQAAMLHAFAH"
     gene            complement(2767..3771)
                     /locus_tag="VIR04_03715"
     CDS             complement(2767..3771)
                     /locus_tag="VIR04_03715"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY9459887.1"
                     /translation="MTRLVEQAARRLADGRIVAFPTETVYGLGADAENPAALAKIFAI
                     KGRPASHPVIVHLSPEADVSYWAKSVPAQARILMQVFWPGPLTLILPRADHISSAISG
                     GQDSIGLRCPSHPVAQALLRQFAQLKSHGQGGVAGPSANKFGQVSPTQAEHVRTEFPE
                     LSSADLMVLDGGPAQVGIESTIVDVSRVEQGIGPVVLRPGHVTAAQISEALGIVLSLP
                     DGAAPRVSGSLKAHYAPRTPLFVLPLDRLLEQVDLAASGPGRVAAVVFDSPSRADSPR
                     VDWCVASADPDAYAQGLYALLRHLDRRGYTRILLQQPPRDTSWQAVNDRIGRAAAAFS
                     "
     gene            complement(3805..4980)
                     /locus_tag="VIR04_03720"
     CDS             complement(3805..4980)
                     /locus_tag="VIR04_03720"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY9459888.1"
                     /translation="MSAASVSFPIMPGDWLGMMGGGQLGRMFCQAAQSMGYKVAVLDP
                     GDQCPAGMVANLHIRTAYDDIAGLERLSERCRAITTEFENVPAASLKKLAERSSVTPS
                     AHAVSIAQDRIAEKSFIERAGVPVAPHSAIYSTSDIDSAPGSLFPGILKTARLGYDGK
                     GQARVTTREQARDAFASFRQAPCVLEALMPLAFEISVVLARGLDGRAIFYIPAVNEHR
                     NGILAISRVAEKLVDASLHQRAANNALAIAEGLNYCGVLCVEFFVLQDGSLVANEIAP
                     RPHNSGHYSINACVTSQFEQQVRVMAGLPLGSTRNLSPSIMLNIMGDAWFDRDTGEKR
                     EPDWLSLLAAPGAKLHLYDKSEARRGRKMGHVTVLGATLDDAHRAAAHVARVLGIAF"
     gene            complement(5041..5568)
                     /gene="purE"
                     /locus_tag="VIR04_03725"
     CDS             complement(5041..5568)
                     /gene="purE"
                     /locus_tag="VIR04_03725"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY9459889.1"
                     /translation="MTLSTESNLKAKPLVGVVMGSASDWDTMKNAATILEDFGVAHEM
                     RVVSAHRMPHDMVDYGSTAVERGLRAIIAGAGGAAHLPGMLAALTPVPVFGVPVPSKY
                     LNGRDSLLSIVQMPKGVPVATFAIGESGAANAALHVIACLATTDSDLRQKLISYRSQQ
                     TEASRAMVVPPHVRM"
     gene            complement(5640..6521)
                     /locus_tag="VIR04_03730"
     CDS             complement(5640..6521)
                     /locus_tag="VIR04_03730"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY9459890.1"
                     /translation="MAQALHQSKIDSLPLLGRGKVRDIYAVGDDKLLIVASDRISAFD
                     VILGDPIPGKGQVLTGLTEFWLKKLAHIVPNHSTDIRPEDVVQANERDQVAGRAMVVK
                     RLKPILVEAVARGYLIGSSWNDYQASGSICGIALPAGLRQARHGHRRIFTPAAKAEMG
                     SHDENVDFNYVVGLVGQDLAEKIRSISLQLYVEAAAFALTRGIMIADTKFEFGLDDKN
                     QLYLMDEVLTPDSSRFWPAADYAVGISPPSFDKQFVRDWLEAHSWNKKPPAPRLPAEV
                     LEKTAAKYREALTRLTA"
     gene            complement(6546..7610)
                     /gene="fba"
                     /locus_tag="VIR04_03735"
     CDS             complement(6546..7610)
                     /gene="fba"
                     /locus_tag="VIR04_03735"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395676.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY9459891.1"
                     /translation="MALVSMRQLLDHAAENGYGIPAFNVNNLEQVQAIMEAANETNSP
                     VIMQASAGARKYAGEGFLKLLIQAAVQTYPHIPVVMHQDHGQSPTVCQGAIALGFSSV
                     MMDGSLKEDGKTIADYDYNLDVTQRVVRMAHKVGVTVEGELGCLGSLETLQGDKEDGH
                     GADGKLTLDQLLTDPEQAADFVRHTQLDALAITIGTSHGAYKFTRKPTGDILSISRIK
                     EIHRRLPNTHLVMHGSSSVPQDLLAEIREFGGDMKETYGVPVEEIQEAIKFGVRKVNI
                     DTDIRLSMTGAIRRFMAENPSKFDPREYLKPARLAAKKLCKRRYEQFGAAGNAGKIKP
                     IALDDMAARYAAGKLAQVVQ"
     gene            complement(7678..8310)
                     /gene="mog"
                     /locus_tag="VIR04_03740"
     CDS             complement(7678..8310)
                     /gene="mog"
                     /locus_tag="VIR04_03740"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY9459892.1"
                     /translation="MTGPMRLIRHHPDELLVGLVSISDRASAGAYADQGIPAMQSWLG
                     QAVLGPCQFVTRLIPDVAAQISSTLIELCDQRGCDLILTTGGTGPARRDVTPEATLAV
                     ATKEMPGFGEQMRQISLKFVATAILSRQVAVVRETADHAALIINLPGQPKAIRETLEG
                     LKDAEGHTLAAGVFAAVPYCIDLIGGPYIDTRESIIKAFRPKSALRPTKA"
     gene            8411..9289
                     /locus_tag="VIR04_03745"
     CDS             8411..9289
                     /locus_tag="VIR04_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY9459893.1"
                     /translation="MTNQRILLAGAGDLCCRTARLLLAQGHQVWALRRRPPTHGDSRV
                     QWLAADLTKPATLSRLPHSISQVVYAPTPDRRIEADYRAVFVDGLRNLLSALDTKALM
                     RFMFISSSAVYGSGDEWVNEDTATNPSGFNGQILVQAEEWLRRELPRTVLFRLTGLYG
                     PQRTELLARLREDRAQVWRHDRRWANRFHIDDAARAVVYLLGLDQIESCYIGTDDHPY
                     PIYELYGALATMLNAPAAFNATAGDPAAGDPAATATTGGKRLSNARWRASGFKPAWPD
                     AIKGYKAIIGQMKRGG"
     gene            complement(9300..>9571)
                     /gene="pgk"
                     /locus_tag="VIR04_03750"
     CDS             complement(9300..>9571)
                     /gene="pgk"
                     /locus_tag="VIR04_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY9459894.1"
                     /translation="SKAGTIVWNGPVGVFEFDAFASGTRAVAQAIADSSGFSIAGGGD
                     TLAAIAKFGVTDKINYISTGGGAFLEFLEAGTLPAVEVLVRRAEK"
BASE COUNT         2203 a         2891 c         2531 g         1946 t
ORIGIN      
        1 gcatagccga tgccatacaa acgcccttgc gaccgcgctt cggcgcgccg cgccaccaac
       61 tctgccaacc cgcctcgttc ctgccccaga gaaattgctt tctgataatc tcccgatccg
      121 attaacgccc cggccggcgc acgataggga aatgcatttg cattaatcag attgcgtcga
      181 atcacgtcca ggggatcgag gttcagttcg accgcaattc gttgtatcag ccgctctagc
      241 gcgaaataca tctgggggcc gccgaatccg cgcactaatg cagatggcgt tttgttagtc
      301 aaaacaactc gattatgcac ttccagattt gacactttat aagcgccggt caatatgccg
      361 tgcatgcgat acagcgtcgc aggttcgggc gcgcgcaaat aggccccgca atcatcgagt
      421 tggtcgtagc gcagagcagt aatttctccg tcaggctgca ctgccgcttc gatcgtggta
      481 acccgattag gggacgacgc cgaagcctgc aaatgttcca atctgtcttc gacccatttt
      541 acgggagccc cagctttacg cgcggcaagg cacagtagag tcacataagt cgacaccgcc
      601 tgcttcacgc caaagctgcc gccggagtca cgtacgaagc gtaaccgcaa ccgattgccc
      661 gatactttga gcgccagtgc aacaacggga tgcaacgcga acggtccctg gaagttcgac
      721 agaacgtcat aaccgtcgtt accggccaga tattccgcga ccaccacaaa acattcaatg
      781 ggagtcgtcg aattgcgggg gtagcgcacc ttgagggaga cacggtgttt tgccgctgaa
      841 aaagccgcat ccggatcacc gtagcaaaaa tgccggtcgc tgattacatt tgaaccgaca
      901 gcgggatgaa gcacgggaac atcaggatgt gtcgcctcct cggagtctat cgcgacaggc
      961 aactgcttat actctacttc gatcaagtca agagcatctt cagcgagata acgactttcc
     1021 gccacgacga ccgctaccgg ttcgccaacg tagcgcactc gttccatcgc cagacaccaa
     1081 tgttccatag gctgtttaac gcctacaaca aaaggaacgg accacttttt tacgtcaacg
     1141 ccggtcagca ccgtccgcac cccggcgcta tcgagcgccg cagcggcatc aatagagaga
     1201 atctctgcgt gcccgtaagg ggaacgcaaa atagccgcgt ataaggtgcc gggcttgaca
     1261 ccaacatcgt cggcgtatgc accacgcccc gtcagtaaag cggcgtcttc aacccgcacc
     1321 ataggacgac caacgtaagg tagctgatca gccccggcag accccgaacc gctcgtcgat
     1381 gtaggggaag tctgattcaa attgtcgcct cactacgttg atatacgcag tgattttata
     1441 tcgatcaatc tacaaagctt ttatcgaatg gtgcgccgat ttacctctga gtctcttttt
     1501 catacggagc caactacggt acttaagact cgccccacct cttcacaagc ccggtttcga
     1561 tctcgaataa atccaaaacc cggcctaccg tgtggttgac aacatcagct accgttgtgg
     1621 gacgggcata aaaagcaggg actggcggca tcacaacggc ccccatctcc gtaacctgtg
     1681 tcatgctgcg gagatgccca agatgcagcg gcgtctctct aaccaacaag accaatcggc
     1741 gtctttcttt caaaattaca tcggccgccc tagtcaacaa attggaagtc acccctgtcg
     1801 atatctcgga caagctccga attgaacaag gcgcgacgac cattcccatc gtcttaaaag
     1861 acccactgga aatagccgcg ccaatatcat cgatcgaata aactttatcg gcgagccttt
     1921 cgatatcaga tacctttaag gcgagctcgt gagccagcgt aacctttgcc gctcgggtca
     1981 taacgagatg tgtttcgatt tcagtttccg cgagcgcctc aagcagtcgt acacccagca
     2041 cgattccgga agcaccgctt attccaacga ttaacctttt aggatcggcc atatcccgtc
     2101 ccatttagta gactgactta cacaagaagg cgctgcttcg ttggagtgaa tacataagta
     2161 attcgattcc gtatataacg aacaacggga ctcaatgagc aaaggcatga agcattgccg
     2221 cctgagcaat cttatctgat aaatgccgta aatcggccat acaggcaaca ctgttcatct
     2281 tgttcgcgta gaagtcaata acgctaccca cggtccgagt gcagccggcc aaattcagcg
     2341 gttcgtcgcc gggcggcaac gcgctgaccc agccagcgag cataccttgc tccacacgtt
     2401 cagcatttaa ctccgctgac ttgacgagat tccttagtcc atctgtatcg agatgcgcgc
     2461 caccaaaccc ttccttcagt cgagtgcgaa ttttgcgcaa aggttccact acttcctcgc
     2521 gccacactgc aatctcccgg tcgcccgcgc taatctcttc tcgcgtaatg gcacggccca
     2581 acctgttcgc tgcccacaag gcaatgagca taacattgac gtcaaccccc gcgctgttct
     2641 gtaatataag acaggcctgt gccacttccg ggtagtcgta aaccgcgagg gaaaaagccc
     2701 agtaatcttc tgcatactgt tgattagacc ccgatgcggc cttttctctt tttttcataa
     2761 acgtctttaa ctaaatgccg cggccgcgcg gccgatgcgg tcattgactg cctgccatga
     2821 cgtgtcccgg ggcggttgct gcagcaaaat gcgcgtataa ccgcgccgat ccagatgccg
     2881 cagcaacgcg tacaagcctt gcgcatacgc atcgggatcg gctgacgcca cacaccaatc
     2941 aacacgaggc gaatcggcgc gcgagggcga atcaaacacc acagcggcca cacgcccggg
     3001 gcccgatgcg gcaagatcaa cttgctccag cagtcgatcc aaaggcagca caaacaaggg
     3061 cgtgcgaggg gcgtaatgcg ccttcaacga ccctgacacg cgtggcgcgg cgccatcagg
     3121 caggcttagg acaataccca atgcctcgct gatttgagcg gcggtgacat ggccgggccg
     3181 cagcacgaca ggaccgattc cttgctctac gcgggataca tcgacgatgg tcgactctat
     3241 tcccacctgc gccggaccgc catccagcac catcaaatcc gccgagctca attcagggaa
     3301 ctcggtgcga acatgctccg cctgtgtggg cgagacttgg ccaaatttgt tggccgaggg
     3361 ccccgccaca ccgccctggc catgcgattt aagctgcgca aactgacgca acaaggcctg
     3421 cgcaacggga tgcgaaggac aacgcaagcc gatgctgtcc tgaccgccgc tgatcgccga
     3481 agaaatatga tcggcacgcg gcaatatcag cgtcaagggc ccgggccaga aaacctgcat
     3541 caaaatgcgg gcctgcgcag gaacggactt ggcccaatag ctcacgtctg cctcgggtga
     3601 caggtgaaca attacaggat ggctcgccgg ccgccccttg atggcgaaaa tctttgccag
     3661 cgcggcagga ttttcggcat cggcgcccag gccgtaaacc gtctcggtcg gaaacgcgac
     3721 gattcttcca tcggcaagac ggcgagccgc ctgctctacc agtcgcgtca tttcgggtga
     3781 aaaagaagaa ggcatgattg ccaattaaaa agcgatgccc aatacccggg ctacgtgcgc
     3841 cgccgcgcgg tgcgcgtcat cgagcgtcgc ccccagcacg gtgacatggc ccattttgcg
     3901 accgcggcgg gcctcggact tgtcataaag atgcagtttg gcgccgggcg cggccaacag
     3961 acttaaccag tcgggctcgc gcttttcgcc ggtatcgcga tcaaaccacg catcgcccat
     4021 aatattaagc ataatcgacg gactcagatt gcgcgtgctt ccgagcggca aaccggccat
     4081 cacgcgcact tgctgttcaa actggctggt gacgcaagcg ttgatggagt aatgaccgct
     4141 attgtgcggt cgcggggcaa tttcatttgc gaccaaactg ccgtcttgaa gaacaaaaaa
     4201 ttccacgcat aatacgccgc aataattcaa gccctccgca atcgccaatg cgttattggc
     4261 cgcgcgttga tgcaacgagg catcgacgag tttctcggcc acgcgagaga tggccaatat
     4321 accgttgcgg tgttcgttga cagccggaat ataaaaaatt gctctgccat caagccctcg
     4381 cgctaaaacc actgaaattt caaacgccag cggcatcagc gcttcaagaa cacaaggcgc
     4441 ttgtctaaac gacgcaaacg catcccgcgc ctgttctcgc gttgttactc tggcctggcc
     4501 cttgccgtcg tatcccaggc gcgctgtctt gagaatgccg ggaaacaacg acccgggcgc
     4561 cgagtcgata tcgcttgtcg agtaaatcgc gctgtgcggt gccaccggca cgcccgctct
     4621 ttcgataaag gatttttcgg caatgcgatc ctgcgcaatt gacaccgcat gcgcggatgg
     4681 cgtaacgctg gagcgttcgg ccagtttttt caaacttgcc gcgggcacgt tttcaaactc
     4741 ggtcgtgatg gcgcggcaac gctcggacaa tcgctctaaa ccggctatgt cgtcatacgc
     4801 ggtgcgaatg tgcaaattgg cgaccatgcc ggccggacac tggtcgcccg gatcaagcac
     4861 cgctaccttg tagcccatac tttgcgccgc ttgacaaaac atgcggccca attgccctcc
     4921 ccccatcatt cccaaccaat ctcccggcat aatcggaaac gacacggaag ctgcactcat
     4981 aacgatgacc tgatctgaaa gtggaaggga caaaagtaaa atcgcaagaa gcgtattgcg
     5041 ctacatccgg acgtgcggcg gaaccaccat ggcgcgcgag gcctcggttt gttgcgatcg
     5101 gtaagagatc aacttctgac gcaagtcgga atcagtcgtc gccagacatg caatgacgtg
     5161 cagggccgcg ttggccgcgc cggactcgcc aatcgcaaag gttgccaccg gcacgccttt
     5221 aggcatttgc acgatggaaa gcagagagtc ccggccattc aaatatttgg acggcacagg
     5281 aacgccgaaa accggaactg gcgtcaatgc cgccaacatg cccggcaaat gtgccgctcc
     5341 gccagcaccc gcaataatag cccttaaacc acgctcgacg gccgtcgaac cataatcgac
     5401 catatcgtgc ggcatgcggt gagccgacac cacccgcatt tcatgcgcca cgccgaaatc
     5461 ttccagtatg gtggcagcgt ttttcatcgt atcccagtcg ctggcggaac ccatgacaac
     5521 gcccaccaat ggtttcgctt ttaaattgga ttcagtcgat agagtcatat tcaatcggtc
     5581 agttataaaa cgcaatggtc catttcaggg tttcggccaa ctgccggcag ccggcggtcc
     5641 tacgccgtca atcgtgtcaa tgcttcgcgg tacttcgcgg cggtcttttc caaaacttcg
     5701 gccggcaggc gcggcgctgg cggtttttta ttccaggaat gcgcctcgag ccagtcccgc
     5761 acaaactgtt tgtcgaacga cggggggctg atccccacgg cgtagtcggc cgccggccaa
     5821 aaacgggacg aatccggggt cagcacctca tccatcaggt aaagctggtt tttatcatca
     5881 aggccgaact cgaacttggt atcggcaatc atgatgccgc gcgtcaaggc gaacgcagcg
     5941 gcttcaacgt ataactgcaa agaaatcgaa cggatttttt cagccagatc ctggccgacc
     6001 agccccacca cgtagttgaa atccacgttt tcgtcatgcg agcccatctc ggccttggca
     6061 gcaggcgtaa atatccggcg gtggccgtgc ctggcctggc gcaatcccgc cggcagggca
     6121 atcccgcaaa tcgaaccact agcctgataa tcgttccagc tcgaaccgat caggtagccg
     6181 cgcgccaccg cttcaacgag aatcggctta aggcgtttga caaccatcgc gcggcccgca
     6241 acctggtcgc gctcgttggc ctgcacgaca tcttcggggc gaatgtcggt cgagtgatta
     6301 ggcacaatat gcgccagttt ctttaaccag aactcggtca gtccggtcaa tacctgacct
     6361 ttgcccggaa tagggtcgcc caaaatgaca tcaaacgccg aaatgcggtc gctggcgacg
     6421 ataagcagct tgtcgtcgcc cacggcgtat atatcgcgca cttttccgcg ccctaataaa
     6481 ggtaaagaat cgatcttcga ttgatgcaaa gcctgtgcca cgttaaatcc ttgaaacgaa
     6541 atcgtttatt gaaccacttg cgccagcttg cccgcggcgt aacgcgccgc catgtcgtcg
     6601 agggcaatcg gcttgatctt gccggcattg ccagcagcgc caaactgctc ataacgccgc
     6661 ttgcaaagtt ttttggccgc cagccgcgct ggtttaagat attcgcgcgg gtcgaatttg
     6721 gaggggtttt cggccatgaa acggcgaatt gcccccgtca tggataaacg aatatcggtg
     6781 tcgatattca cttttcgcac gccgaacttg atggcttcct gaatctcctc gaccggcacg
     6841 ccataagttt ccttcatgtc gccgccaaat tcccggatct cggccaacaa gtcttgcggc
     6901 acgctggaac tgccgtgcat aaccaaatgc gtattgggca aacggcgatg gatttccttg
     6961 atgcgcgaga tcgacaaaat atcgccagtg ggcttgcgag tgaacttgta cgcgccatgg
     7021 ctcgtaccaa tggtaatagc caacgcatca agctgggtat gccgcacgaa atcggccgcc
     7081 tgttcagggt cggtgagcaa ctgatccagg gttaatttgc catcggcgcc atgtccgtct
     7141 tctttgtcgc cctgcaaggt ttcaagcgaa cccagacaac ccaattcccc ttcgacggtt
     7201 acgccgacct tatgggccat tcgcaccacg cgttgcgtca cgtcgaggtt atagtcgtaa
     7261 tccgcaatcg ttttgccatc ctctttcaac gagccatcca tcattacgct gctgaaacca
     7321 agcgcaatgg ctccctggca gacggtcggc gactgcccgt ggtcctggtg catgaccact
     7381 ggaatatggg ggtaagtctg aactgccgct tgaatcagaa gcttcagaaa gccttcgccc
     7441 gcgtatttgc gtgcccccgc cgacgcctgc atgatcacgg ggctgttggt ttcgttggcc
     7501 gcctccatga ttgcctgaac ctgctcgagg ttattcacgt taaaagcggg gatgccatag
     7561 ccgttctcgg cggcatggtc gagcaactga cgcatggaaa ctagggccat gaagacctcc
     7621 tgaaagctaa ataaaaagga attgccgcat tttaaccttg aacgcgtcaa cgggctttca
     7681 ggctttcgtt ggccgcagcg ccgatttcgg gcgaaacgct ttgataatcg actcgcgcgt
     7741 gtcgatatac ggcccaccga taaggtcaat gcaatagggc accgccgcaa ataccccggc
     7801 cgccagcgta tggccttcgg catctttcag gccttcgagg gtttcgcgga tcgccttggg
     7861 ctgacccggc agattaatga tcagggcggc gtgatcggcc gtttccctga cgaccgccac
     7921 ttgccgcgac agaatggccg tcgcaacgaa cttcaggctg atctggcgca tttgctcgcc
     7981 aaaaccaggc atttccttgg tcgccaccgc caatgtcgcc tcgggcgtta catcgcgccg
     8041 cgcgggaccc gtgccgcccg tagtcagaat caagtcgcaa ccccgttgat cgcaaagctc
     8101 gatcagggtg gacgatattt gcgcggcaac atcgggaatt agccgtgtaa cgaactggca
     8161 aggccccagc acagcttgcc ccagccagga ttgcatggcg ggaattccct gatccgcata
     8221 ggctcccgcc gaggcgcggt cggaaatgga aacaagcccc acgagcaatt cgtcgggatg
     8281 atggcgaatc agacgcattg gcccggtcat gattgccttg cgccgcagct gaatcgcata
     8341 ccgccccctg tgtttaatgg ttgattgcac taatatagtt catcacgaaa tcgcggcacg
     8401 cgcggaaaac atgacgaatc aacgtatttt actagccggc gcgggcgatt tatgctgtcg
     8461 cacagccagg cttcttcttg cacaaggcca tcaagtgtgg gcactgcggc gcaggccgcc
     8521 gacccatggt gattcgcgcg tccagtggct agcggccgac ctgacaaaac ccgcgacact
     8581 aagccgcctt ccgcactcca tcagtcaggt ggtgtacgct cccacgcctg atcggcgcat
     8641 cgaagccgac tatcgcgctg tctttgtgga tggcttgcga aacttgttat ccgctctgga
     8701 tacgaaggcg ttgatgcggt ttatgtttat ttcttcaagc gccgtctatg gctctggcga
     8761 tgagtgggta aacgaagaca cggccaccaa cccgtcggga ttcaatggcc aaatactggt
     8821 tcaagccgag gagtggctta ggcgagaact accccggact gtcctatttc gtctgacggg
     8881 gctgtatggg ccacaacgaa ccgagctatt ggcgcgcctg cgggaggatc gcgcgcaggt
     8941 ctggcggcac gatcgacgct gggccaaccg ctttcacatc gacgacgcgg cgcgcgcagt
     9001 ggtctacctg ctaggcctgg atcaaatcga gtcgtgttac atcggaacgg atgaccatcc
     9061 ctacccaatt tacgagctgt acggcgcgct ggcgaccatg ctgaacgcgc ccgccgcctt
     9121 caatgccacg gccggcgatc cggcggccgg cgacccggcg gccacagcga caacaggcgg
     9181 caaacgtctg agcaacgccc gttggcgcgc cagcggcttc aaaccggcgt ggcccgatgc
     9241 gatcaagggt tacaaagcca ttatcggcca gatgaaacgc ggcggataac acaacagggt
     9301 tacttttcgg cccggcgcac caaaacctca accgccggca aagtccccgc ctccagaaac
     9361 tccaaaaagg cgccgccgcc tgtcgaaata taattaattt tgtctgtgac gccgaatttg
     9421 gcgatggcgg caagcgtatc gccgccgccc gcgatggaaa accccgagga atcagcgatg
     9481 gcttgggcga ccgcccgagt gccgctggcg aacgcgtcga attcgaaaac accgacaggg
     9541 ccattccaga caatcgtgcc ggctttagaa a
//
LOCUS       DATCXU010000168         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_722626,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000168 DATCXU010000000
VERSION     DATCXU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722626"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VIR04_03755"
     CDS             <1..376
                     /locus_tag="VIR04_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 peptidase family dipeptidase"
                     /protein_id="HEY9459895.1"
                     /translation="EILPALRRHLDRQGFSKVKIGSTGETMFQATRIDPDDPWVQWVA
                     ESLRNTSGKKTAILPNLGGSLPNDIFTDILGLRTVWVPHSYPGCSQHAPNEHLPPALL
                     REALCIMTGLYWDMGDGNTPRQ"
     gene            complement(378..1433)
                     /locus_tag="VIR04_03760"
     CDS             complement(378..1433)
                     /locus_tag="VIR04_03760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY9459896.1"
                     /translation="MHNAQIEQAVQFAVSHETAWDRDVNGVWGVHQIDTPPWNRLLGP
                     VHSRGPVSGTIVLDGRQLTSWGEPLRADLTFSIAKTYLALLAGVAHDRGLLPDVDERV
                     ARRVPGLGFDKGRNAEITWLHLLQQTSEWEGECFGVPDQVDRYRAVAFGAVPAGKKGE
                     TRPLQAPGGYWEYNDVRINILALALLHLFRQPLPDVFREAITRPIGASENWQWVGYDN
                     AWVQIDGKRMQSVPGGSHWGGGMSISSVDQALIGQLFINDGQANGRQVLSADWLRFMR
                     TPCALAPYYGGLIWLNHEPRIFPSIPASSYFGIGAGKSFMWVEPERRMVVIVRWIDGD
                     YANEFFGKVLQAVDAGV"
     gene            complement(1560..3011)
                     /locus_tag="VIR04_03765"
     CDS             complement(1560..3011)
                     /locus_tag="VIR04_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEY9459897.1"
                     /translation="MKKSTGVAGIVIVLGIAYVGASWYIGKQAQTAIGQAVAQTNQRL
                     VGMLGPDLGGNGPKVVITDYQRHVFSSDVVYSLELKDSNGKLLVFKLKDHLQHGPLPF
                     DAVRSGDLTPMMAQGSAVMVPTTATQKWFDSLKGESPVTATTRVGFAGTAQSTWKFKP
                     LSLSEGKDTLEFGGGAVEVKFSNSFKDSTASGLFASFHVIAAANNEDLLAKGIKFNSA
                     TTTLRANDVQLKSDAAIDDLVMASGLDPTIHIKTMKVGLSSVRANNLLDGALHYDFGQ
                     VAIGQADLGHIAVAVKGQRLDTEALAALRAEYEGIKAKHHVKDDEDFLLTDAESVTMH
                     ARLAALLASDPSVSIDSLVWENDKGRSAIALNVDLMRPADKTTVDTGLDALLAQIVKR
                     VNLNLSISRPMFIQAVAQSQSQPGSADNEQADQLGGLLFDQYAGRLRQLGLIKMDKDN
                     AVSAIQYENAMVVANGQKMTVPQFIQRVLGLLM"
     gene            3158..3775
                     /locus_tag="VIR04_03770"
     CDS             3158..3775
                     /locus_tag="VIR04_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY9459898.1"
                     /translation="MATPFEFYFDFSSPYGYFASTQIDALAAEFNRNVRWRPILLGAI
                     FKKTSAAPLTELPLKGNYALRDFARTAALFGIPYSQPARFPIATVGAARAMLWIEQNA
                     GETKAKESAHRVYRAYFAEGQDIDNLGTIKELADKAGIDATLLADAIQQESVKTALRN
                     QIETAMDKGVFGSPFIFIDGEPFWGFDRFDYIRKWLRNETKPKAR"
     gene            3939..>4388
                     /locus_tag="VIR04_03775"
     CDS             3939..>4388
                     /locus_tag="VIR04_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9459899.1"
                     /translation="MTQTTRLFKARHLACAAFIALAGSLSAHAATFPDHSITLVVPYP
                     PGGTTDISGRTLADAMQKQLKQTIVVENRAGAGGVIGMGVVAHAAPDGYTLGLGTIGT
                     QSINQFLYKNMSYDPEKDLTPIALVITTPNVIAAQTSSKIKTVSDLIA"
BASE COUNT         1008 a         1349 c         1108 g          923 t
ORIGIN      
        1 cgaaatcttg cccgccttgc gccgtcacct tgaccgccag ggcttttcaa aggtcaagat
       61 cgggtccacc ggtgaaacca tgtttcaggc aacgcgcatc gatcctgacg acccgtgggt
      121 gcaatgggtt gccgagtcgc tgcgcaacac cagcggcaag aaaactgcca ttttgcccaa
      181 tctgggcggc tcgctgccta atgatatctt cacggacata cttggcctgc gcacggtgtg
      241 ggtgccacat tcctatcccg gctgctccca gcacgctccc aacgaacacc tgccgcccgc
      301 attgttgcgc gaggccttat gcataatgac gggcctgtac tgggatatgg gtgacggcaa
      361 tacgcccagg cagtgaccta cacgcccgca tcaaccgctt gcaacacctt gccgaagaat
      421 tcattggcat aatcgccgtc aatccagcgt acgatgacga ccatccggcg ctcgggctcc
      481 acccacatga aggatttgcc cgcgccgatg ccaaaataac tggaagcggg aatactggga
      541 aatatgcgtg gctcgtgatt aagccagatc agtccgccgt aatacggagc caacgcacaa
      601 ggagtgcgca taaaccgcag ccagtcggcc gacagcactt gtcgcccgtt ggcttgccca
      661 tcgttgataa acaactgccc tatcagcgcc tgatcgacac tgctgatcga catgccgccg
      721 ccccaatggg aaccgccggg aaccgactgc atgcgtttgc cgtctatctg cacccaggcg
      781 ttgtcatagc ccacccactg ccagttctcg ctggcgccta tggggcgcgt aatggcttcg
      841 cggaatacgt ccggcaaagg ctgccggaac aagtgcaata aagccagcgc aagtatattg
      901 attcgaacgt cgttatattc ccaataacca cccggggcct gcaaaggccg cgtctcgcct
      961 ttctttccgg cgggcacagc gccaaacgcg acggcacggt aacgatccac ctgatccggc
     1021 acaccaaagc attcgccctc ccactcgctg gtttgctgca acaaatgcaa ccaggtaatt
     1081 tccgcgttcc ggcccttgtc aaagccaagc ccgggcacac gccgggccac gcgctcgtcc
     1141 acgtcgggca atagtccgcg gtcatgcgcg acgccagcca acaacgcgag gtatgtcttg
     1201 gctatgctga aggtcaagtc cgcgcgcagc ggctcgcccc aggatgtcag ttgccggccg
     1261 tccagcacaa tagttcccga cacggggccg cgtgaatgga ccggaccgag caaacgattc
     1321 cagggcggcg tatctatttg atgaacgccc catacgccat tcacgtcgcg atcccaggcg
     1381 gtctcgtgag agaccgcaaa ctggacggct tgttcgatct gtgcattgtg catttaaagc
     1441 ccttttttgg atttgcccac cactaaaatc gccgcgccag gacgctgacc gtacagacgg
     1501 caagcgttgt gccgccgatc atagcattcc cggcacaacg ccgcgacggc cattgcggcc
     1561 tacatcagca atccgagaac ccgctgtatg aactggggaa ccgtcatttt ttggccattg
     1621 gcgacgacca tggcgttttc gtattgaatc gccgagacgg cgttgtcctt gtccattttt
     1681 atcaggccaa gctggcgcaa ccgcccagca tactgatcaa aaagcaagcc gcccaattga
     1741 tcggcctgct cgttatcggc ggagccaggt tgcgactgcg actgtgcaac agcctggatg
     1801 aacatgggtc tggaaatgga taaattcaaa ttgacgcgtt tgacgatttg cgcaaggagc
     1861 gcatccagcc cggtgtccac ggtggtttta tccgccggac gcatcaaatc gacattcagg
     1921 gcgattgcgc ttcgtccttt gtcattctcc cataccagcg agtcgatcga caccgaagga
     1981 tcagacgcca gcaacgccgc caatctggcg tgcatcgtca cgctttcagc gtcggtcaat
     2041 agaaagtctt cgtcatcctt gacatggtgt tttgccttaa tcccctcgta ttcggcgcgc
     2101 aacgccgcca gcgcttccgt gtccagtcgc tgccccttga ccgccacggc aatatggccc
     2161 aggtcggcct gcccaatcgc gacctggccg aagtcataat gcagcgcgcc atcaagcaag
     2221 ttattcgccc gcacgctgct caaaccaact ttcattgttt taatgtgtat ggtcggatca
     2281 aggcccgatg ccatgaccag atcgtcaatg gccgcgtcgc ttttaagttg cacatcattt
     2341 gcgcgcaagg ttgtggtggc gctattgaat ttaattccct tggcgagcaa atcctcgtta
     2401 ttggcggccg cgatcacgtg aaacgatgca aacagaccgc tggccgtact gtctttaaag
     2461 ctattggaaa acttgacctc cacggcgccg ccgccgaatt caagcgtgtc cttgccctcg
     2521 gacaggctca gtggtttgaa tttccatgtt gactgggctg taccagcaaa gcccactcgc
     2581 gtggtcgccg ttacgggcga ttcaccctta aggctgtcaa accacttttg agtcgccgtg
     2641 gttggaacca tgaccgcgct accttgcgcc atcataggcg tcaggtcgcc cgagcgcacc
     2701 gcgtcaaaag gcagtggccc gtgctgaagg tgatccttca atttgaaaac cagaagcttg
     2761 ccattggagt ccttcagttc aaggctatat acaacatcgg acgaaaagac gtggcgctga
     2821 tagtcggtaa ttacaacctt cgggccattg ccgcccaagt ccggccccag catgcccacg
     2881 aggcgctgat tggtctgcgc gaccgcctgc ccgatcgctg tttgcgcctg tttgccgata
     2941 taccaggagg cgcccacgta ggcgatgccc agcacaataa caatacccgc taccccagtg
     3001 ctcttcttca tcaatcatcc aatcattttt caattcgaac ccaggccgac aatcccggcc
     3061 cgcacttaaa atagtaacca acaatcggcc ttgtttccgc attttccgat tattattttt
     3121 gtccgcacat ttactaaatc gacacgctcg ggatcctatg gctactccct ttgaattcta
     3181 ttttgatttc tcttctccat acggttattt cgccagtacg caaatagacg ccctggccgc
     3241 cgaattcaat cgcaacgtac gctggcgccc gattttactt ggggccatat tcaaaaagac
     3301 gagcgcggcg ccgctaaccg aacttccact caaaggcaat tatgcattgc gcgatttcgc
     3361 ccgcaccgcg gcgcttttcg gcatccccta ctcgcaacct gctcgcttcc ccatcgccac
     3421 cgtcggcgcc gcgcgcgcca tgctctggat cgaacaaaac gccggagaga cgaaagccaa
     3481 agagtccgcc caccgcgttt accgcgccta ttttgccgaa gggcaagaca ttgataatct
     3541 tggcacgatc aaagaactgg cagacaaagc gggaatcgac gcaacgctac tggcagacgc
     3601 aattcagcaa gaaagcgtca agacagcgct tagaaaccaa atagaaacgg ctatggacaa
     3661 aggcgtgttc ggctcgcctt tcattttcat tgatggcgaa ccgttctggg ggttcgaccg
     3721 cttcgattac ataagaaagt ggctgcgaaa cgaaacaaaa cccaaggcac gatgacaccg
     3781 gtcatatcgt cgccgcgcat gccttcgttg tatagttagc ctcgggccga aaatgccggg
     3841 acggtaacct gacaacatcg ttctttaacg cgaaccggaa ccaagcccat tattacaaaa
     3901 ttgcattcat cgcaaataaa accaagaagg agacaacaat gacacaaacc acccgccttt
     3961 ttaaggcaag acaccttgca tgcgccgcat tcattgctct ggccggctcg ctgtcggcac
     4021 acgcggcaac gtttccggat cattccataa cgctcgtggt gccttacccg ccggggggca
     4081 caaccgatat ctcgggacgc acgctggccg acgccatgca aaaacagctt aaacagacca
     4141 ttgtcgttga aaatcgcgcc ggtgcgggcg gtgtcatcgg catgggtgtt gtggcgcacg
     4201 ccgcgcccga cggttatacc ctggggctgg ggacgatcgg tacgcaaagc atcaaccagt
     4261 tcctgtacaa aaacatgtcc tatgaccccg aaaaagattt gacgcccatc gctctggtca
     4321 taacaacgcc gaacgtgatc gcagcgcaaa catcatcaaa gattaaaacc gtgtccgatc
     4381 ttattgcc
//
LOCUS       DATCXU010000169         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_25313,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000169 DATCXU010000000
VERSION     DATCXU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25313"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VIR04_03780"
     CDS             complement(<1..257)
                     /locus_tag="VIR04_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY9459900.1"
                     /translation="MSESEIIRVLETALLCADQPMSLAELDKLFVLEDISHDDLRRFL
                     ALLQLDWTEKGLELVNVASGWRFQSRPLMHSYLARLNPEKPP"
     gene            332..408
                     /locus_tag="VIR04_03785"
     tRNA            332..408
                     /locus_tag="VIR04_03785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Met,seq:cat)
     gene            1214..2350
                     /locus_tag="VIR04_03790"
     CDS             1214..2350
                     /locus_tag="VIR04_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY9459901.1"
                     /translation="MPETFSLIQDLNKIDPVQWDHLAGDHPFLRHEFLLALDASQCAA
                     ARTGWAPHYLLLHRGQRIAAAMPLYLKSHSRGEYVFDMAWARAFEQHGINYYPKLLGA
                     VPFTPVPGPRLLARTHADRVLLARQAIEITRQNELSSLHILFPQRADREALREAGFMF
                     REDVQFHWRNQSYRTINDFLSSLNQQKRKKINQDRKKSAQAGIRFRWLQGDEIDGKSL
                     AFFFKCYCRTYLEHGNAPYLNYDFFLRLHQTMKDSLLIILAEKDNRAIASALNIRGPD
                     TLYGRYWGTTQFISGLHFETCYMQAIEFCIDRRIASFEGGAQGEHKLSRGLMPVKTYS
                     AHWIRDRQYAQAIADFLALETPAVDDYVDELKAHSPFKEKTHEP"
     gene            complement(2356..2919)
                     /gene="phaR"
                     /locus_tag="VIR04_03795"
     CDS             complement(2356..2919)
                     /gene="phaR"
                     /locus_tag="VIR04_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY9459902.1"
                     /translation="MTQPQPPVPIRLIKKYPNRRLYDTQTSAYITLSDVKQLVLENES
                     FEVVDAKSGEDLTRSILLQIILEEESGGMPMFSPAALSQIIRFYGHATQGVMGTFLEK
                     NIQAFIEIQDRMTEQSKGLYGNQYGPEAWAQFMNIQTPVLQNMMNNYIDQSKNLFVQM
                     QDQMHDRTRSMFAGFPFAAGVPKDPKK"
     gene            complement(2958..3695)
                     /gene="phbB"
                     /locus_tag="VIR04_03800"
     CDS             complement(2958..3695)
                     /gene="phbB"
                     /locus_tag="VIR04_03800"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY9459903.1"
                     /translation="MSAKLVYVTGGMGGIGTSICHRLAKEGHTVVAGCGPSRDFKQWL
                     DEQAALGYKFHASVGNVSDWDSTVAAFSKVKQDIGPIDILVNNAGITRDGLFRKMSRD
                     DWRAVIDTNLNSLFNVTKQVIEGMVDRQWGRIINISSVNGQKGQFGQTNYSTAKAGIH
                     GFTMALAQEVASKGVTVNTISPGYIGTDMVRSIRPDVLEKIVATIPVKRLGDPEEIGS
                     MVAWLASDDAGFATGADFSLNGGLHMG"
BASE COUNT          930 a         1134 c          990 g          950 t
ORIGIN      
        1 gggggctttt cgggattcag acgcgcaagg tacgaatgca taaggggacg gctttggaac
       61 cgccagccgc tcgcaacatt aaccagttcc agccctttct cggtccagtc aagctgcagt
      121 aacgccagaa accgcctcag atcgtcgtga gaaatgtctt ccagtacaaa caacttatcc
      181 aactcggcta acgacatcgg ttggtccgca cacaataatg cggtttccaa tacgcggatg
      241 atctcggatt cactcattga tcagtaagca gatgatttct gccgtggttt gcgaaaaact
      301 tcgattcgga gtactatcag cattcttcag acgcggggtg gagcagtctg gcagctcgtc
      361 gggctcataa cccgaaggtc gtaggttcaa atcctgcccc cgcaaccagg tttcagatat
      421 gggccagttg tcaaaccaga caattggccc atagtcattt gaaaacccgt ttgaaaaccc
      481 gtttcaattt gaaaacccgt ttcaaagcct gttctcaaat tcgtttgcaa attcgttcgc
      541 agacccagtt tcaaacccag cgcgccgcta tctccggttg cgcgcgccgc cgccgataca
      601 ctggcggagc gtgtttgcat ccggatggcc ccgcgcgcat ccgatgcggg cacacacaat
      661 aaaataggtc gttgacttgc tctgatactg ggcatgcttt gcgtgttaag aaatattggc
      721 cgggcgatgc cagctatttg gaacatcggc gttcaaatac ttggggcgaa tcggcaacgc
      781 ctcgccaaat ggcgagggcc gggatgcgta ccgctgttgt ttgaaacctg tgcaagttat
      841 agtgctgcgg ccggagaagt aaaatcgtca cggcccataa tcagcaggcg cctgtcggct
      901 tgttgcattt gattaagaaa ctttaccgac aaccaagtcg cgctttttag cagcaacccc
      961 gccttgccta ccagcgttaa ttgctactat tacaccaccg cgtcgcgcat attgcaatac
     1021 tgcaagtgat aaatagcgcc tgccgcgaca gcgctgttgg cagccagacc acgcttgttc
     1081 taatttcacc tagtatgtcg tccattcggc gcccgtggtt gaaccgcaac atccgcctcc
     1141 cacgccgctg ccatttacgc gtgcgcccgg gtccgctaaa ctgaatccgc cgatcccaat
     1201 tacaacagca ttcgtgcctg aaaccttttc acttatccag gacctgaaca agatcgatcc
     1261 cgtccaatgg gatcatctgg caggcgacca tccttttttg cgccacgaat ttttgcttgc
     1321 gcttgacgcc agccaatgcg cggcggctcg cacaggctgg gcgccgcatt acttgttgct
     1381 tcaccggggc cagcgcattg cagccgcaat gcctctttat ttgaagtctc actctcgcgg
     1441 tgaatatgtg ttcgacatgg cctgggcccg cgccttcgaa cagcacggca tcaactacta
     1501 tccaaagctg ctcggcgccg ttccgttcac gcctgtgccg gggccacgct tattggcgcg
     1561 cacacatgcc gatcgagttc tgcttgcccg gcaggccatc gaaattacac gccagaatga
     1621 actatcgtcc ctgcatattc tttttccgca acgcgccgac cgcgaagcgc tgcgcgaagc
     1681 cgggttcatg ttccgggaag acgttcaatt ccattggcgc aatcagtcat accgaaccat
     1741 caacgatttc ctgtccagtc tgaaccagca aaagcgcaaa aaaataaatc aggaccggaa
     1801 aaaatcggcc caggcgggga tccgctttcg ctggttgcag ggcgatgaaa tcgacggcaa
     1861 aagccttgct ttctttttca agtgctactg ccgaacatat ctggagcacg gaaacgcgcc
     1921 ctacttgaac tacgactttt ttttacggct gcatcaaacg atgaaagaca gcctgctgat
     1981 cattctggct gaaaaagaca atagagccat cgcttcggcc ttgaatattc gcggccccga
     2041 taccctgtac ggccgttact ggggaaccac tcaattcatt tcaggcttgc acttcgaaac
     2101 ctgttatatg caagcaatcg aattttgcat cgatcgccgg atagcctcgt tcgaaggcgg
     2161 cgctcaaggc gaacacaagc tgtcgcgggg cctgatgccg gtcaaaacgt attcagccca
     2221 ttggatacgc gacagacaat atgcccaggc gattgccgat tttttggccc tggagactcc
     2281 cgcggtcgat gactacgtcg atgaactaaa ggcgcatagt cccttcaaag aaaaaacgca
     2341 cgaaccctga catccttatt ttttaggatc tttgggcacg ccggcggcaa aggggaaacc
     2401 tgcaaacatg gagcgtgtcc gatcatgcat ttgatcttgc atctgcacaa aaagattctt
     2461 gctttgatcg atgtaattat tcatcatgtt ttgcaacacg ggcgtttgaa tgttcataaa
     2521 ctgcgcccag gcctcggggc catattgatt gccatacaaa cccttggatt gctcggtcat
     2581 ccgatcctga atttcaataa acgcctgaat atttttttcg agaaacgttc ccattacgcc
     2641 ctgggtcgca tgtccgtaaa agcggataat ttgcgacaag gctgcaggcg aaaacatagg
     2701 catgccgcca ctttcttcct cgaggataat ctgcagcaaa atactgcgcg tcagatcctc
     2761 gccagacttc gcgtcgacca cctcgaagct ctcgttttca agcaccaatt gtttaacatc
     2821 ggacaatgta atataggcgc tcgtctgcgt gtcgtacaag cgacgattgg gatacttctt
     2881 gatcaggcga atgggtacag gaggctgcgg ttgcgtcata acagtgtcaa taaggaagga
     2941 agttgagcgg tcgggtttta acccatatgc aagccaccgt tcagcgagaa gtccgcgccg
     3001 gtcgcgaaac cggcgtcgtc ggatgccagc caggcaacca tggagccaat ttcctcgggg
     3061 tcgccgaggc gcttcacggg gattgtagcc acaatttttt caagaacatc agggcgaatg
     3121 gatcgaacca tgtcggtgcc gatatagccc ggcgaaatcg tattgacggt aacaccttta
     3181 ctggcgacct cttgcgccag agccatggtg aaaccatgaa ttcccgcctt ggccgtagaa
     3241 taattggttt ggccgaactg ccccttctga ccgttcaccg aactgatgtt gatgatgcgc
     3301 ccccactggc ggtcaaccat cccttcaata acctgcttgg ttacattaaa caggctgttc
     3361 agattcgtat cgataacggc gcgccaatcg tcgcggctca ttttgcgaaa caacccgtcg
     3421 cgcgtaatgc cagcgttatt gaccaggata tcgatcggtc caatatcctg ctttactttc
     3481 gagaatgcgg caacggtgga atcccaatcg gacacattgc ccactgaagc atgaaacttg
     3541 tagcctaatg ccgcctgctc gtcgagccat tgcttaaagt cgcgacttgg gccgcatccg
     3601 gccacaacgg tgtggccttc tttagccaga cggtgacata ttgaagtccc gattcccccc
     3661 atcccacctg tcacataaac caatttggcg ctcatgttta tctcctgtgc gggtattttt
     3721 tagtgtcgcg aataatgtcc atgattgcgt atgacgcggc ttatcctgct tgtaactgta
     3781 ccactatagt tgacattgtt gcggccatgg acggcgatgt ccggcgcgca gcgaatcgac
     3841 ggcgtcttac atagctcgaa ccttcacata ccgcccaggc gccgcttcaa tcggcgcatt
     3901 gcgcgtattg cccaacgcgg cctttgctcg tttctgtccg cccgaatggg aagccagcca
     3961 acgttgccag tccggccacc agctacctgg gtgctgcacc gcgt
//
LOCUS       DATCXU010000170         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_252866,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000170 DATCXU010000000
VERSION     DATCXU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252866"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VIR04_03805"
     CDS             complement(<1..656)
                     /locus_tag="VIR04_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY9459904.1"
                     /translation="MAAKPLGFSAWLADRTGHIETVLDRLLPPSSTPPENLHHAMRYA
                     VLGGGKRVRAMLVYAAGEASLQAGSPASAQEIALDYAAAAVEMVHAYSLVHDDLPCMD
                     NDALRRGRPTVHVRFNEATAMLAGDALQPLAFELLAQMPIAPALIVQATQILAHAAGS
                     LGMAGGQAIDCDSIGLSLTRDQLQTMHSLKTGAMLDASVLLGGIVAGAGSTLRQGLET
                     "
     gene            complement(660..932)
                     /locus_tag="VIR04_03810"
     CDS             complement(660..932)
                     /locus_tag="VIR04_03810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY9459905.1"
                     /translation="MTSNTTIPEPAASQAALPHDFETALAQLEALVAQMEGGSLPLDE
                     SLAAYERGVELAKVCQQRLDAAEQQVKVLQDNLLKPLSDVADVEKE"
     gene            1104..2246
                     /locus_tag="VIR04_03815"
     CDS             1104..2246
                     /locus_tag="VIR04_03815"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY9459906.1"
                     /translation="MSDIGREALLAPTQAQLCVNHYFDHSIFAQEQALIFKKSACYVG
                     HEKLVPEVGDWRTLTHENAGRVLVHNENGVELLSNVCRHRQAIVLGGEAGDIASAKNA
                     SGNLGDTGGNIVCPVHRWTYSNQGRLIGAPHFPSTPCLNLQKFTLKNCHGLLFEGPRD
                     PARDMAELFKRPEFDFSEYVLDHVEVHRCRYNWKTFIEVYLEDYHVGPFHPGLGQFVS
                     CDNLKWEFSPHYSLQRVGVHSSLTQPGTDAYRRWHERLLEYRGGGTPDFGAIWVTYFP
                     THMIELYPHVVVLSTLHPVSPQETVNIVEFYYPEEIVAFERELVEAQRAAYMETALED
                     DEIGERMDAGRKALLDRGVNETGPYQSPLEDGMMHFHEWYRRTMSS"
     gene            complement(2243..3154)
                     /locus_tag="VIR04_03820"
     CDS             complement(2243..3154)
                     /locus_tag="VIR04_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9459907.1"
                     /translation="MQSLWMLLACAAFAILGACVKVASDFDASLSQVVIFRGVPSIIL
                     LFGWARLGHRTIAPSSWKLHFWRNVSGIASMWLGFYALAHLPLSIAVSLNYTAPLFIA
                     GWMLVWGGSQRDPVRIIAVVFGFLGVIGVLRPSIENNNLLAASLGLLAGALAAVAMMQ
                     IRELGRVGEPEWRTVLIFSGCVWLSGIVGVMIQGWRDIGLTAWIALTGVGIAGLVCQL
                     AMTRAFGLGSTLLTAALQYATIIFAAILGILFWGDRPDLLAWGGMALIIGSGLLSVWR
                     TLGEDRLMRGRAATAAGAAPAGAAAVD"
     gene            3315..4196
                     /gene="htpX"
                     /locus_tag="VIR04_03825"
     CDS             3315..4196
                     /gene="htpX"
                     /locus_tag="VIR04_03825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY9459908.1"
                     /translation="MKRIFLFLLTNLAVLIVLSATLNVLGVGRYLSANGLNLSQLLIF
                     SVIVGFTGSIISLLISKWMAKASTGASTIDPQAPANAREAWLIDTVHQLADRAGIGRP
                     EVAVYDGAPNAFATGAFKNDALVAVSTGLLESMTEEEITAVLGHEISHVANGDMVTLT
                     LVQGVINTFVIFLARVVGYFVDQIVLKNEKGAGLGYYATVIACEIVFGILASIIVAWF
                     SRQREYRADAGSAHLLGAREPMIRALERLGGAESGDLPKALQAFGISGGKAVSALFAS
                     HPPIAARIHALQTAQRI"
BASE COUNT          992 a         1259 c         1135 g          828 t
ORIGIN      
        1 tacgtttcaa gcccctggcg cagagtggag cccgcgccgg cgacgatgcc gcctaaaaga
       61 acgctggcat cgagcatcgc acctgttttc aagctatgca tggtttgcaa ttggtcgcgt
      121 gttaacgata acccgatgct atcgcaatcg atagcctggc ctcccgccat ccccaggctg
      181 ccggccgcgt gcgccaaaat ctgcgtcgcc tgtacaatca aggctggcgc gatcggcatc
      241 tgcgcgagca gctcgaacgc cagtggctgc agcgcatcgc ccgcaagcat ggcagtggcc
      301 tcgttaaaac gcacgtgaac cgtaggacgc ccacgccgca atgcatcgtt gtccatacag
      361 ggcaaatcgt catgcaccag cgaataggca tgtaccattt caacggccgc cgccgcgtaa
      421 tccagagcaa tttcttgtgc cgacgcgggt gatcccgcct gcagtgaggc ctcgcccgcc
      481 gcatacacca acatggccct cacccgcttc cccccgccca gaaccgcgta acgcatggcg
      541 tgatgcaagt tttcgggtgg cgtcgaagac ggggggagca accgatccaa aacggtttca
      601 atatgtccgg tccggtccgc aagccaagcg gaaaaaccaa gtggcttagc ggccatgctt
      661 cattcctttt cgacgtcggc aacgtcgctt aagggcttga gcaaattgtc ctgtaaaacc
      721 ttaacctgct gttcagcggc atcaagacgc tgctggcaga ccttggccag ttcaactccc
      781 ctttcataag cggccaatga ctcatccagc ggcaacgatc cgccttccat ctgcgccacc
      841 agcgcctcca actgcgccaa tgccgtctcg aaatcatgcg gcagcgccgc ttgagaggcg
      901 gccggctcag gaattgtggt attgcttgtc attgcgactc tcccaatgta aataaatggc
      961 cgcgccacat ttgttttaga ctcaggctga attgtacgag atgagtcatc tcttggctaa
     1021 atttgcttat tccggagcat tattcgaaag aataagtaag gtacaatgct tggttcttca
     1081 cgttttatct agggcggggg attatgtccg acatcggtcg ggaagcgctc ttggcgccga
     1141 cgcaagccca actttgcgtc aatcactact tcgaccactc catttttgcc caggaacaag
     1201 cgcttatttt taaaaaatca gcgtgttacg tcgggcacga aaaactggtg cccgaagtgg
     1261 gcgactggcg cacactgaca catgaaaacg ctggccgcgt tctggtgcac aacgagaatg
     1321 gcgtagagct tttgtcgaat gtttgccgcc accgccaggc catcgtactg ggcggcgaag
     1381 cgggcgacat cgccagtgcc aaaaacgcaa gcggcaactt gggcgacacc ggcggcaata
     1441 tcgtctgtcc ggttcaccgc tggacctaca gcaatcaagg ccggctcatt ggcgcgccgc
     1501 actttcccag cactccgtgc ctgaacctgc aaaagttcac acttaaaaac tgccatggtc
     1561 tgctctttga aggtccgcga gacccggccc gtgacatggc tgagctcttc aagcgccccg
     1621 aattcgactt ttcagagtat gtgcttgacc acgtcgaagt gcaccgatgc cggtacaact
     1681 ggaaaacatt tattgaggtc tacctggaag actaccatgt gggtccgttt caccccggcc
     1741 tgggccaatt tgtaagctgc gataacctga aatgggaatt ttcgccgcac tacagcctgc
     1801 agcgcgtcgg cgtgcatagt tcgctgacgc agccaggcac cgacgcctac aggcgctggc
     1861 atgaacgcct gctggaatac cggggcggcg gtacgcccga tttcggcgca atctgggtca
     1921 cctactttcc cacccacatg atcgaactct atccgcatgt ggtcgtactc tcgacactgc
     1981 accccgtcag tccgcaagag accgtgaata tcgtcgagtt ttattacccc gaggaaatcg
     2041 tcgcgttcga acgggaactc gtcgaggcgc agcgcgccgc atatatggaa actgcgctcg
     2101 aggacgatga aatcggcgaa cgcatggacg ccggccgcaa agcactactg gacaggggcg
     2161 tgaatgaaac cggcccatac caatcgccac tggaagacgg catgatgcat tttcacgaat
     2221 ggtaccgccg cacgatgagc agctaatcga ccgcggcagc gcctgccggg gcagcgccgg
     2281 ccgccgtagc agcccggcca cgcatcagac ggtcttcgcc gagcgtgcgc cagacggaca
     2341 gcaggcccga gccaataata agagccattc caccccaggc gagcagatca ggccggtccc
     2401 cccaaaatag aatgcccagg atcgcggcaa aaataatggt ggcatattgc aaagcggcgg
     2461 ttaataaagt cgagcccaga ccaaacgccc gagtcatggc cagttggcaa accaggccgg
     2521 caatgccgac gccagtcaag gcaatccagg ccgtcagacc aatatcgcgc catccttgaa
     2581 tcatcacccc gacaatacca ctaagccaga cacagccgga aaaaatgagc acggtgcgcc
     2641 attcgggctc gcccacgcgc cccagttccc ggatctgcat catggccacc gccgcaagcg
     2701 cgcctgccag caggcccagc gacgcggcca gcaaattatt attctcgata ctgggacgaa
     2761 gtacaccgat cacccccaga aatccgaata caacggcaat aatgcgcacc ggatcgcgct
     2821 gacttccgcc ccaaacaagc atccagcccg caataaacaa gggagccgtg taattaaggc
     2881 tgaccgcaat ggaaagaggc aggtgggcca gggcgtaaaa acccagccac attgaggcaa
     2941 tgcccgacac attgcgccaa aaatggagct tccagcttga tggagcaata gtgcggtggc
     3001 caagccttgc ccacccgaag agcagaataa tggatggaac accgcgaaat atcaccactt
     3061 gagacaacga ggcgtcaaaa tcagaagcga ccttaacgca ggcgcccaga atcgcgaatg
     3121 cagcgcacgc cagcaacatc cataaagact gcatgcaatc aaccgaaatc gggttaaaaa
     3181 ttgacgaagc caatactata ctctgctaca ttacttctat attcggcggc gcgtcgcgct
     3241 tgttttaaat ggccaaaccg cgacttgcat gaaacatata cgcttattac caagtgcctt
     3301 gggggacctt cctaatgaaa cgaatattct tgtttttgct gaccaatctt gctgtgttga
     3361 tcgtgctcag cgcgacattg aatgtattgg gcgttggccg ttaccttagc gccaatgggc
     3421 tcaatctatc gcagttgctg attttttcgg tgatcgtcgg gttcaccggg tcgattattt
     3481 cgttgttgat cagcaaatgg atggccaagg ccagcacggg cgcatcgacc attgatccgc
     3541 aagctccagc caacgcgcgc gaggcatggc ttatcgatac cgtgcaccaa ttggccgatc
     3601 gagcgggcat cgggcggccc gaagtcgccg tctacgacgg agcacccaat gccttcgcta
     3661 ccggagcgtt caaaaatgat gcattggtgg cggtgtccac cggcttgctc gagagcatga
     3721 ccgaagaaga aataaccgct gtgttgggac acgaaatatc gcacgtcgcc aacggcgaca
     3781 tggtcacact aactctggtg cagggcgtta tcaatacctt tgtgattttc ctggcgcggg
     3841 tcgtcggata tttcgtcgac cagattgtgc ttaaaaacga aaaaggagcc gggctgggct
     3901 attacgcaac cgttatcgcg tgcgaaattg tcttcggaat tttggcttcg atcattgtgg
     3961 cctggttttc gcgccagcgg gaataccgcg ccgatgcggg ctcggcgcat ttgctcgggg
     4021 cgcgcgaacc catgattcgc gcgttggagc gcctgggcgg agccgaatca ggcgatctgc
     4081 ccaaggcgct ccaggccttc ggaatctctg gcggcaaagc cgttagcgca ctattcgcct
     4141 cgcatccgcc cattgcggct cgcatccatg cgctgcagac cgcccagcgc atttaagcaa
     4201 ccggcagccc aggg
//
LOCUS       DATCXU010000171         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_91913,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000171 DATCXU010000000
VERSION     DATCXU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91913"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VIR04_03830"
     CDS             complement(<1..787)
                     /locus_tag="VIR04_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459909.1"
                     /translation="MSDLQIGLISLGVVLILVVVSFNWWQDRRVRQKMQEHFPETEQD
                     PLMGGLPPVASARREPGLGLHDPQSETLADDTAEVDPNCEAVIDIEFSQAVASDQLHA
                     ALQAIHKVGNKPVRLFVEREGGGHRARLRPGEFYKSMQLAVLLANRSGPLTDIEWSHL
                     WVIAQGLAERFDGIIEAPEQALVLDRARRLDELCAGLDAQVGLALRLGGTRPVVEMTQ
                     AAKDVGFLSRGAQLAWMADSGVARFTLLFDGAHAGEVQSAGVDR"
     gene            complement(831..>2527)
                     /locus_tag="VIR04_03835"
     CDS             complement(831..>2527)
                     /locus_tag="VIR04_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY9459910.1"
                     /translation="AVYIAPDVATALASRAGLPPGAAYVVKEGHLIDQNSLRFYAPDS
                     EQSGLLARQREIENLGKEVKAMRLISDQLVADVVRAEADWQQVSAAMAPARMRVGELT
                     ERLHDVQLEHSRLQQQAQQSGERAARLREDLAEIALQSEELRAGKEEAVARFETFDIE
                     LGEHQSRFAEAEIMGEDLFNRAERARQNLRDLERAVQESEFAERALQTRIADLVRNLQ
                     LAVDQAKRADDELNGLQGELFELDASATQAGLQDALELRAQREESLGLARTQLDNLAA
                     LLRSADEERATLELSLEPRRAHITELQLQEQAARLAIEQFAEQLDAHEVDREQLRQTL
                     AERPEQWRRTNWLQAEVQRISRLVEGLGPVNLAALDELDAARERKGFLDAQYQDLNTA
                     IETLEDAIRKIDRETRALLQDTFDTVNRHFGELFPLLFGGGEARLSITGDEILDAGVQ
                     VMAQPPGKRNSTIHLLSGGEKALTATALVFALFKLNPAPFCLLDEVDAALDDANTERY
                     ANLVVSMSGQTQFLFISHNKIAMQAAKQLVGVTMQEQGVSRIVAVDIDAALQLAEV"
BASE COUNT          536 a          811 c          673 g          507 t
ORIGIN      
        1 tacggtcgac gccagccgat tgaacctctc cggcatgggc gccatcaaac aacaaggtaa
       61 aacgggcgac tcccgaatca gccatccagg ccaactgcgc gccgcgagac aaaaacccga
      121 cgtctttcgc cgcctgggtc atctccacca ccggacgcgt tcctcccagc cgcaatgcca
      181 ggccaacctg tgcgtccagc cctgcgcaca actcatcgag ccggcgggcg cgatcaagaa
      241 caagcgcttg ctcgggagcc tcgatgatgc catcgaaacg ctcggccaaa ccttgggcga
      301 tcacccaaag gtgcgaccat tcgatatcgg tcagcgggcc gctgcgattc gccagcaaga
      361 cagccagttg catggattta tagaattcgc ctggacgcaa tctggcgcgg tgaccgcccc
      421 cttcgcgttc gacgaacaaa cgcaccggct tatttccgac tttgtgtatt gcctgtagcg
      481 ccgcatgcaa ctgatcgctg gcgactgcct gagaaaactc aatatcgatg acggcctcgc
      541 aattgggatc gacctcggcc gtatcgtcag ccaatgtttc gctctgaggg tcatgcaaac
      601 ccagtccagg ttcgcgccgg gcggaggcaa cgggcggcag gccgcccatt aacggatctt
      661 gctccgtctc ggggaagtgc tcctgcattt tttgccggac gcggcgatcc tgccaccagt
      721 taaagcttac caccaccagg ataagcacaa cgcccaggga aatcagacct atttgcaaat
      781 cactcatttt tagccccgac gcatcaaagc acgtttaact atcagcactg ttatacctca
      841 gccaattgaa gcgcggcgtc aatatcgacc gcaacgatac gggaaacccc ctgttcctgc
      901 atggtgacgc caaccaattg cttcgcggcc tgcatggcaa ttttattatg ggaaataaat
      961 aaaaactgag tttgcccgct catgctgacg accaaattgg cataacgctc ggtattggca
     1021 tcgtcgagcg cggcgtcgac ctcgtcgagc aaacaaaaag gcgccggatt aagcttgaac
     1081 aacgcaaaca caagggctgt ggcagtaagc gctttctctc ccccggacag caaatggatc
     1141 gtgctattgc gtttgcccgg cggttgcgcc atgacctgaa ctccagcgtc aagaatctca
     1201 tcgccggtaa tcgacaatct ggcttctccg cccccaaaaa gaagcgggaa caattcacca
     1261 aaatgacggt tgaccgtatc gaatgtgtcc tgcagcaatg cgcgcgtctc gcggtctatt
     1321 ttgcgtatgg cgtcttcaag cgtttcgatg gccgtattca gatcctgata ctgagcgtca
     1381 agaaacccct tgcgttcgcg cgcggcgtca agctcgtcca gcgccgccag attaaccggc
     1441 cccagacctt cgaccagccg cgaaatccgc tgcacttcgg cctgcagcca attggtccgg
     1501 cgccactgct ccggacgctc ggccagcgtt tgccgcagtt gttcacgatc gacctcgtgc
     1561 gcgtccagtt gttcggcaaa ctgttctatc gccaggcgcg ccgcctgttc ctgcaactga
     1621 agctcggtaa tatgcgcccg gcgcggctca agcgaaagct cgagggtggc gcgctcttca
     1681 tcggcgctac gcagcaatgc cgcgaggttg tcaagctgag tgcgagccag gccaagggac
     1741 tcttcacgtt gcgcccgcag ttcaagcgcg tcttgcaagc cggcctgcgt cgccgatgca
     1801 tcgagctcga acaattcacc ctgcaggccg ttcaattcgt catcggcccg tttagcctgg
     1861 tcgaccgcca attgcaggtt ccgtaccagg tcggctattc gcgtttgaag agcgcgctcg
     1921 gcgaattcgg actcctgcac ggcacgctcc aggtcgcgca ggttttgacg cgcgcgttca
     1981 gcccgattaa acaggtcttc gcccattatc tccgcttcgg caaagcgcga ctgatgttcg
     2041 cccaactcaa tatcaaaagt ttcgaaacgc gctacggcct cttctttgcc tgcgcgcagt
     2101 tcctcagact gcaacgcgat ctccgccagg tcttcgcgca gacgcgccgc tcgttcgccc
     2161 gactgttgag cctgttgctg caagcgggaa tgctcgagct gcacatcgtg caggcgctcg
     2221 gtcagctctc ccacacgcat gcgcgcgggc gccatggcgg ccgaaacttg ttgccagtca
     2281 gcttcggccc gcacaacatc ggccacaagc tggtcggaaa taagccgcat ggcttttact
     2341 tccttgccca ggttttcgat ttcacgctgg cgcgccaata acccggactg ctccgaatca
     2401 ggcgcataaa accgcagact attttgatcg atcagatgac cctctttgac gacataggcc
     2461 gcgcccggcg gcaggccggc gcgcgaagcc agcgccgtgg cgacatcagg ggcaatataa
     2521 acagcac
//
LOCUS       DATCXU010000172         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_295981,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000172 DATCXU010000000
VERSION     DATCXU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295981"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VIR04_03840"
     CDS             complement(<1..405)
                     /locus_tag="VIR04_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY9459911.1"
                     /translation="MITNELNADPASTVVPGYILGIAPYKAGKPIEELAREFKLDPAS
                     IIKLASNENPLGLPESARRAMMDAAGSLARYPDSNGFELKAALARRYGVPADWITLGN
                     GSNDLLELASLALLSPGSAAVYSQYAFVVYRLA"
     gene            complement(418..1503)
                     /gene="pheA"
                     /locus_tag="VIR04_03845"
     CDS             complement(418..1503)
                     /gene="pheA"
                     /locus_tag="VIR04_03845"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY9459912.1"
                     /translation="MDNSLQTQLLPLRKKIDSLDEQILDFLNQRARVALEVGKLKKKF
                     DEDGAVLKPEREVAVIRRLQELNRGPFTAQAVQAIWSEIISTCRGLESVLTVAYLGPQ
                     GSFSEQAAFEQFGHAIVKLRCNSFDEVFRAVEAGQANVGMVPVENSSEGAVNRTLDLL
                     LNSSLKVLGERSIKIHHCLMTQSGTLDGVTRVMAHPQALAQCQSWLTQNHPYLVRDAA
                     SSNAEAARVASQDPVVAAIAGTSAASAWNLQIVASGIQDDPQNRTRFLAVGAIDTLPS
                     GNDKTSLILAVPNRSGAVYEMLAPMAANGVSMTRFESRPARTGQWEYYFYVDLLGHHD
                     DPAVARTLSELKKQVAFFKVLGSYPIQ"
     gene            complement(1496..2623)
                     /gene="serC"
                     /locus_tag="VIR04_03850"
     CDS             complement(1496..2623)
                     /gene="serC"
                     /locus_tag="VIR04_03850"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY9459913.1"
                     /translation="MRPWNFSAGPAVLPLEVLEQAAAEMTDWHGSGMSVMEMSHRGKQ
                     FTQICDEAEADLRELLSVPDDFAVMFMQGGATAENAILPLNLIGRGGSNKADYVLAGR
                     WSSKSYQEAQRYGDIAVAASSGSSSLLNGIEQEPWTWFPETSQWRVRPEAAYLHICSN
                     ETIGGIEFVDWPEMGSLGAPDVTLVVDASSHILSRSIDFSKAGLVYAGAQKNAGIAGV
                     TMVMVRRDLIGHALPICPSAFDYANVAQAQSKFNTPPTYAIYIAGLVFKWLKKRGGVA
                     AIEQANIAKARTLYAYLDQSDFYTNRIAPNVRSRMNVPFFLKDESLNAKFLAQAEAAG
                     LMQLKGHKSVGGMRASIYNATPIEGVNALISMMQEFERRHG"
     gene            complement(2623..>3836)
                     /locus_tag="VIR04_03855"
     CDS             complement(2623..>3836)
                     /locus_tag="VIR04_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY9459914.1"
                     /translation="RLQRLTGLEQDKIVGEYKTVMDAIADLLDILARPERVTTIIGDE
                     LAAIKTEFSINAKDSRRSEIERHATELDTEDLITPMDMVVTLSQSGYIKSQPLSEYRA
                     QRRGGRGKQATAMKENDWVDQLFIANTHDYLLCFSDRGRVYWLKVWEVPQGSRNSRGR
                     PIVNMFPLLADEKITVVLAIKEFSEDHFVFMATSQGTVKKTPLSDFSNPRKGGIIAVA
                     LDENDYLIGADLTDGKHDVMLFSDGGKAVRFDENDVRPMGRTARGVRGMKLDEGQAVI
                     SLLVAGDETESVLTATANGFGKRTSILEYTRHGRGTKGMIAIQTSSRNGKVVGAVLVV
                     PSDDVMLITTAGVLVRTRVSEIREMGRATQGVTLINVDENSTLSGVRRVVESDADTDD
                     DSESAEPKEDL"
BASE COUNT          863 a         1122 c         1010 g          841 t
ORIGIN      
        1 ggccagacgg tacacgacaa acgcatattg cgaatacacc gccgccgatc cgggcgacaa
       61 cagcgccaac gacgccaatt cgagcaaatc gttggacccg ttccccaatg taatccagtc
      121 ggccgggacg ccataacgcc gggccagcgc tgctttcagc tcgaagccgt tggaatcggg
      181 ataccgcgct agcgagccgg ccgcatccat catggcgcgg cgcgccgatt cgggcaaacc
      241 cagcgggttt tcgttcgatg ccagcttaat gatgctggct ggatcgagct tgaattcgcg
      301 cgccagttct tcgatcggtt tgcccgcttt ataaggggcg atgcccaaaa tgtagccggg
      361 cacaaccgtg ctggcgggat cggcgttaag ctcgttggta atcatgctcg cgcgtcctta
      421 ctgtatggga taggaaccca gcaccttgaa aaaagcgacc tgttttttca actcggatag
      481 cgtccgcgcc actgccgggt cgtcgtgatg cccaagcaaa tcaacataaa aataatattc
      541 ccactgaccc gtgcgcgccg gccgggattc gaaacgcgtc atggaaactc cgttggcggc
      601 catcggcgcc aacatttcgt atacggcccc ggaacgattc ggcacagcca gaatcaagct
      661 ggttttatca ttgccactgg gcagggtatc aattgccccc acagccaaaa agcgggtgcg
      721 attttgaggg tcatcctgaa tcccggaggc gacgatttgc agattccatg ccgaagcggc
      781 gctcgtcccg gcaatcgccg cgacgacggg atcctgcgac gccacacgcg ctgcctcggc
      841 attgctcgaa gccgcgtcgc gcaccagata ggggtgattt tgcgtcaacc aggattgaca
      901 ctgagccaaa gcctgagggt gcgccatgac acgggtaacc ccgtcgagcg tgccggattg
      961 cgtcatcaga caatggtgaa tttttatcga acgctcgccc aacactttaa gcgatgaatt
     1021 cagcagcagg tcgagcgtgc gattaaccgc gccctcggat gagttttcaa cgggaaccat
     1081 gccgacattg gcttgtccgg cttcaacagc gcgaaagact tcatcgaacg agttgcagcg
     1141 taatttaaca atggcgtgcc cgaattgttc aaatgccgcc tgttcggaga atgaaccttg
     1201 cgggcccaaa taagccactg tgagcacact ttccaggccg cggcaagtgg aaataatttc
     1261 gctccaaata gcttgcacgg cctgtgcggt gaacggcccc cgattcagct cctgcagacg
     1321 gcggataaca gccacctcgc gctcgggctt taaaactgca ccgtcctcat cgaatttctt
     1381 tttcagcttc ccgacctcga gcgctacgcg agcgcgctgg ttaaggaaat cgagaatctg
     1441 ctcatcgaga gaatcaattt tcttgcgtag cggcaataac tgggtttgca aagagttatc
     1501 catggcgccg ttcaaattct tgcatcattg aaattaaggc attgacgccc tcgatcgggg
     1561 tcgcgttata gatagatgcc cgcatcccgc ccacgctttt atgtcctttg agctgcatta
     1621 atcccgctgc ttcagcctgc gccaggaact tggcgttcaa cgattcgtct ttcaggaaaa
     1681 agggaacatt catgcgcgaa cgaacatttg gcgcaattcg attcgtgtaa aaatcggatt
     1741 gatcaagata ggcatagagc gtgcgcgcct tggcgatatt ggcctgctct atcgcagcaa
     1801 caccgccacg ttttttgagc cacttgaata ccaggcccgc gatataaatt gcataggttg
     1861 gcggcgtatt gaatttggat tgggcctgcg cgacattggc gtaatcaaat gccgaagggc
     1921 atatcggcag cgcgtggcca atcaaatcgc ggcgcaccat taccatagtg acacccgcaa
     1981 tcccggcgtt tttttgagcg cccgcataaa ccaggccagc cttggaaaaa tcgatggatc
     2041 gggacaatat atgcgaagaa gcgtcaacca ccagagtcac gtcgggagcg cccagcgatc
     2101 ccatttctgg ccaatcgacg aattcaatcc caccgatggt ctcgttgctg catatatgca
     2161 aatacgccgc ctcgggacgc acgcgccatt ggctggtttc gggaaaccag gtccagggct
     2221 cctgttctat gccgttcaac aaagacgagc tgccgctgga tgcagcaacc gcaatatcgc
     2281 cgtaacgctg ggcttcctgg tacgacttgc ttgaccaccg gcccgccagg acataatccg
     2341 ctttattcga gcccccgcga ccgataagat tcaagggcaa aatggcattt tcagcggttg
     2401 ccccaccctg catgaacatc accgcgaaat catcgggcac ggacaacaac tcgcgcaggt
     2461 cagcttcagc ttcatcgcaa atctgggtga actgcttgcc tcgatgactc atttccatga
     2521 cagacatgcc gctgccatgc caatctgtca tttcggcggc cgcctgttcc agcacttcaa
     2581 gcggcaagac cgcggggcct gccgaaaaat tccacggtcg cattacaaat cttccttggg
     2641 ttcggcggat tccgaatcat cgtcggtatc cgcatcgctt tctaccactc gccgcacacc
     2701 cgaaagcgta ctgttttcgt cgacattgat aagcgttaca ccttgcgtag cgcggcccat
     2761 ttcacgaatt tcagagacac gcgtgcgcac cagaacgccc gcagtcgtaa tcagcatgac
     2821 atcgtcggac ggcacaacca gcaccgcccc caccaccttg ccgttgcgcg aactggtctg
     2881 aatggcaatc atgcccttgg tgccgcgtcc gtgacgcgtg tattcaagga tcgacgtgcg
     2941 tttgccaaaa ccgttggccg tcgcggtcag gacgctttcg gtctcgtcgc cggcaaccag
     3001 cagggaaatg acagcctgtc cttcatccag cttcatgccc cggacaccgc gcgctgtccg
     3061 gcccattggg cgcacgtcat tctcgtcaaa gcgcacagcc ttgccaccat cggaaaacaa
     3121 catgacatcg tgcttgccat ctgtaagatc cgcgccaatc agataatcgt tctcgtccaa
     3181 agctacggcg ataattccgc ctttgcgcgg gttggagaaa tcggacaatg gcgtcttttt
     3241 aaccgtgccc tgggacgttg ccatgaatac aaaatggtct tcgctgaatt ctttaattgc
     3301 cagcaccacg gtgatctttt catcggcaag cagagggaac atattgacga tggggcggcc
     3361 gcgcgaattg cgcgagcctt gcggcacttc ccagacctta agccagtaca cgcggccccg
     3421 atctgaaaaa cacagtaaat aatcgtgcgt gttggcaata aacaactgat cgacccagtc
     3481 gttttctttc atggcagtgg cttgcttgcc gcgtccgccg cgccgttgcg cacggtattc
     3541 agacaaaggc tgacttttta tgtagccgct ttgcgacagt gtcaccacca tatccatggg
     3601 ggtgatcaag tcctcggtat cgagttcggt tgcgtggcgt tcgatctcgg aacggcgcga
     3661 atctttggcg ttgatcgaaa attcggtctt tatggcggca agttcgtcgc cgataatcgt
     3721 ggtgacccgt tccggccgcg ccagaatatc gagcaggtcg gcgatcgcat ccatgacggt
     3781 tttatattcg cctacgattt tgtcctgttc cagaccggta agacgctgca gacgca
//
LOCUS       DATCXU010000173         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_422457,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000173 DATCXU010000000
VERSION     DATCXU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422457"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VIR04_03860"
     CDS             complement(<1..520)
                     /locus_tag="VIR04_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9459915.1"
                     /translation="MQEASWSELLWGRNGLRSLALAGGVFLHAIDVYIVATILPSVVQ
                     DIGGLEYYAWNITLFVVASIMGSALSPKAIDAFGSRRAYLVSIGVFLLGTVVCALAPS
                     MAWLLLGRTCQGFGGGVLLGLSYALIRILFDERLWSRVVALISGMWGMATLAGPAVGG
                     IFAQMGHWRLAFW"
     gene            complement(589..792)
                     /locus_tag="VIR04_03865"
     CDS             complement(589..792)
                     /locus_tag="VIR04_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY9459916.1"
                     /translation="MSTDQRLIEIEIKVTRQEDLTQELSRQVYQQQKKIDELQRLCAR
                     LLRRFDEAAGAGDPFTPEKPPHY"
     gene            complement(845..1315)
                     /locus_tag="VIR04_03870"
     CDS             complement(845..1315)
                     /locus_tag="VIR04_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY9459917.1"
                     /translation="MISLTQFKRWVTVIAASVILASACGTALAQNVKAQPDNALALRH
                     IMQDLDKNTQAISHGIEEKDWPLVAKLAALIADHPTPPLGEKVRILTYLGTDALAFRG
                     LDKDAQAAATAIQKAADKEDGPGVIAAFGKMQAACMACHNSYRAPFLQHFYGKQ"
     gene            complement(1400..1684)
                     /locus_tag="VIR04_03875"
     CDS             complement(1400..1684)
                     /locus_tag="VIR04_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459918.1"
                     /translation="MKSDKTGVAKRLTFSLVMLLGVGGCAVYSPPPAYTYFDSNGQTV
                     YASPVEPAPVYVAPAYVAPAYIGPPVFFNFGFSSGGGRRGHFHGGRGHWR"
     gene            2067..>2414
                     /locus_tag="VIR04_03880"
     CDS             2067..>2414
                     /locus_tag="VIR04_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEY9459919.1"
                     /translation="MKLTPSKLCAAFGAMLAVSFASVTAAAPIVVKFSHVVTDATPKG
                     QGALKFKEVAEKLLPGKVTVQVFPSSQLFGDAKEMEAVLLGDVQFIAPSLSKFDRYTK
                     KVQLFDLPFLFDDM"
BASE COUNT          603 a          686 c          596 g          529 t
ORIGIN      
        1 accaaaacgc cagccgccaa tggcccattt gggcaaatat gccgccgacg gccggtccgg
       61 caagcgtggc cattccccac ataccggaaa tcagcgccac aacgcgagac cagaggcgct
      121 cgtcaaacaa aatgcggata agcgcatagc taaggcccag caacacgccg ccgccgaaac
      181 cctggcaggt cctgccaagc agcaaccacg ccatggaggg ggccaaagca cagactaccg
      241 tacccagcaa aaaaacaccg atagacacca aatatgcgcg acgcgaaccg aaagcatcta
      301 tcgccttggg cgagagtgcc gatcccatga tggatgccac gacaaataga gtgatgttcc
      361 aggcgtagta ttccagcccg ccgatgtcct gcaccaccga aggcaaaatg gtggcaacga
      421 tataaacgtc gatggcatgc aggaacacac cgcccgccag cgccagcgag cgcaggccgt
      481 tgcggcccca taataattca gaccaggacg cttcctgcat cgtgcccaca ataccttttt
      541 tttaaacgcg cggatgacgt cgattttacc gggcttgggc gattctgatt aataatgagg
      601 cggcttttca ggagtaaacg ggtcacccgc gccagccgct tcatcaaaac gccgcaacaa
      661 acgggcgcat agcctttgca gctcgtcaat ctttttttgc tgctggtaaa cctggcggct
      721 gagttcctgt gtcaagtctt cctggcgggt aactttgatt tcaatttcga ttaaacgctg
      781 atcagtcgac accgtacccg ccctttgtgg aaaccatcag tttagaccag gggcacgccg
      841 gaatttactg cttgccgtaa aagtgctgta gaaatggcgc gcggtaacta ttatgacaag
      901 ccatgcaggc tgcctgcatt tttccgaatg ccgcaatcac cccggggccg tcttctttgt
      961 cggcggcttt ttgaatggct gttgcggccg cttgcgcatc cttgtcaagg ccgcgaaacg
     1021 cgagcgcatc cgtgcccaaa tacgtcagaa tgcggacttt ctcgcccaaa ggaggcgtgg
     1081 gatggtcggc gatcagcgct gccaatttcg caaccagcgg ccaatccttt tcttcaatgc
     1141 catggcttat tgcctgcgta ttcttatcca ggtcctgcat aatatggcgc aaagccaatg
     1201 cattgtcggg ctgcgctttg acattttgtg ccaacgccgt cccgcacgcc gacgccaaaa
     1261 ttacgctggc ggcgataaca gtaacccatc gtttgaactg cgtaagagat atcatcgatt
     1321 cacacctgcc gacggcaata gtaagtaact acttactatt ttaccatcag agcaggcaga
     1381 accgatactg cgcactctct taacgccagt gtccgcgccc gccgtgaaag tgtccgcgac
     1441 gtccgcctcc cgagctaaag ccgaagttga aaaacacggg aggcccgatg taagccgggg
     1501 caacgtatgc gggcgccaca taaaccggcg cgggttcaac cggcgaggca tacaccgtct
     1561 gtccattgct atcaaaataa gtataagcgg gcggcggcga ataaacggcg catccaccca
     1621 ctccaagaag cataacgagc gaaaaagtaa ggcgtttcgc cacaccggtc ttgtcggatt
     1681 tcataagcgt tggacgtgat ccctttttgt ttaacgttta acgattgttt tattggtatt
     1741 ggacatcatt cattaagact agttcgaagc gctgataact tgttacacga tggtaagtta
     1801 tcgaaataga ccgctttcgt tcagtttttt gtcaggtttt accctatgat gaattttggc
     1861 aaaccgctcg actgtaaaaa cgcaagctat tgttatcatt gaacaaaact gcatatttcg
     1921 ggggcttttt cagctatcga aacaggcggc cgctcctttg acaaacaaat atcggcccat
     1981 tacagtcccc ggattgcccg tccacgggaa cgaactgcgt gcgcatcgcg gcgcacgcta
     2041 tcacaaaaag ccggagaatc ccacccatga aactaacccc gtccaaacta tgtgccgcgt
     2101 tcggcgcaat gcttgctgtt tcgttcgcca gcgtgacggc ggctgcgccc atcgtcgtca
     2161 aattcagcca cgtcgtaacc gacgccacgc ccaaaggcca gggcgcgctc aaattcaagg
     2221 aagttgccga aaaattgttg cctggcaaag tcactgtcca ggtattccca agctcgcaac
     2281 tatttggcga tgccaaagaa atggaagccg tactgcttgg cgatgtgcag ttcattgctc
     2341 catcattatc caagttcgat cgctacacca agaaagtcca gttattcgat ttgccatttc
     2401 tgttcgacga tatg
//
LOCUS       DATCXU010000174         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_592956,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000174 DATCXU010000000
VERSION     DATCXU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592956"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VIR04_03885"
     CDS             <1..733
                     /locus_tag="VIR04_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459920.1"
                     /translation="AYVGVSLTLMGVSFWWLLILVPLIMLPLGFVVERLLIQKVFHHK
                     DRHILTLLLTLGLALVLEDLFKVIYGPDSIRAEMPISGVAEVAGVLLPNYRLFLMAIG
                     SAIILAVWLIVYRSSWGATVRAAAFDKDMAASLGVPVTRVYAGTFAFGVALAGLAGVL
                     LAPIYSVFPTMGIDFILLAFCVVIVGGMGSIKGTVIAGLVLSQIQSISSLYIPPSWAD
                     PLVFAIMVAVLMIRPQGLFGRLGHV"
     gene            726..1772
                     /locus_tag="VIR04_03890"
     CDS             726..1772
                     /locus_tag="VIR04_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459921.1"
                     /translation="MSNVSPSTLAARTTRMAVQQHERAARNALRYLAYLFIALALVFA
                     AVAGMNGDVFFYRLATEALIFGGFAMSVDLLLGFTGLLSLGHALFFGLGAYVSALILK
                     DLAPSFWLAIAVTLLVASVVGTIAGIIAIRVRGVYFALITFGMAEVVSKAVYNTREIG
                     GSDGIIGIPVVHIDLLFFSVDSNNALSFFLLVLAIIMGLYFLLEAMLQTPFGRLVKAI
                     RANEGRVPFLGFSSQHTKLYVFILASNVAALSGALYPMLRGFVSPELMFFHTSADAVI
                     TVILGGVGTLIGPLYGSVILTTLKSIIGTYTEHQQIVVGALFMLSVIFFPGGLAGYFR
                     PRLNAWLTRREGRS"
     gene            1769..2560
                     /locus_tag="VIR04_03895"
     CDS             1769..2560
                     /locus_tag="VIR04_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459922.1"
                     /translation="MSSANPGGARIPEQPSILEVKNVSVRFGALVAVNAVSFVARRGS
                     VTSVIGPNGAGKSTLFNLISGALTSSSGEVWFDGANVTGWAASRLVRKGLSRSFQITN
                     LFFEISVYENLRLAAQVLEPQSKLLMPLRRSALARQRTEELIEQFKLDDKVHELAGYL
                     SHGEQRRLEIAVALASRPKVLLLDEPTQGMSHADTEETSKLIRSLSRDVSILLVEHDV
                     SLVMDLSDHVVVMAQGAKLADGTPAQIRSNLEVQAAYFGREVSHA"
     gene            2553..3260
                     /locus_tag="VIR04_03900"
     CDS             2553..3260
                     /locus_tag="VIR04_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459923.1"
                     /translation="MLELDGLHTHYGLSHVLQGISLTIEAGEVVLLCGRNGVGKTTVI
                     KTIAGWVAPSSGSVRFDGIEIGGLTPDRICRLGIGLVPEDRRIFPGLTVEENIKLGFL
                     QCPGRAAAQSRRALEETYERFPRLRERREQMGTTLSGGEQQMLAIARVLVGAPKFLLI
                     DEPTEGLAPMIVDEIFTMIDSLRSEGITVLMVEQNVHRALDVSTRFYALERGQIIVQG
                     SASSRADRDDLMKAITV"
     gene            3302..>3573
                     /locus_tag="VIR04_03905"
     CDS             3302..>3573
                     /locus_tag="VIR04_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459924.1"
                     /translation="MSGCRNCSNLPVFKGKIEMIESLNSAAPLTLRRWLRRLASTNRL
                     VVARPDVGLKYQLAAIAKRLDGSKAVLFPRPDGHGIPIVSGFMARR"
BASE COUNT          686 a          821 c         1070 g          996 t
ORIGIN      
        1 cgcctatgtc ggtgtcagcc ttacgttaat gggcgtgagt ttttggtggc tattgatcct
       61 ggttccattg attatgctgc cgttgggatt tgtggtggag cgtttattaa tccagaaggt
      121 gtttcaccac aaggatcggc acattcttac gctgttgctg acattgggac tggcgcttgt
      181 gctcgaggat ttgttcaagg tcatttacgg ccctgattca atccgggcgg aaatgccaat
      241 cagtggagta gccgaagtcg ctggggtgct tcttccgaac taccgcttat ttcttatggc
      301 aatcggaagt gcaattattc ttgccgtatg gctgatcgtt taccgcagca gttggggtgc
      361 gaccgtgcgt gcggccgcat tcgataaaga catggcagct tcgctgggtg ttcccgtaac
      421 ccgtgtctat gcagggactt tcgcgtttgg cgtggctctt gcgggacttg caggggtact
      481 actggcgccg atttattcgg tgttcccaac gatgggcata gattttattc tgctggcgtt
      541 ttgtgttgtc atcgttggtg gtatggggag catcaagggt accgtaattg ctggtctggt
      601 gttgtcgcaa attcagtcga tctcaagctt gtatattcct ccatcttggg ctgatcctct
      661 ggtatttgcc attatggtgg cggtgctcat gatacgtccc cagggtctgt tcggaaggct
      721 gggccatgtc taacgtatca ccttcgaccc tggccgccag gacgacacgc atggcggttc
      781 aacagcacga aagggcggcg cgcaacgcgc tgcggtatct ggcttatttg tttatagcgt
      841 tggcgctggt gttcgccgct gtggcgggca tgaatggaga cgtatttttt tatcgtcttg
      901 cgaccgaagc attgattttc ggcggttttg cgatgagtgt ggatttgctg cttggtttta
      961 cgggactatt gtcgctgggc catgcattgt tttttggact cggtgcctac gtatcggcgc
     1021 tgattctgaa agacctcgct ccgtcgttct ggcttgccat cgcggtgaca ttgttggtgg
     1081 cgagtgtggt gggcacaatt gccggcatca ttgcgattcg ggtgcgcggc gtttattttg
     1141 ccctgatcac gtttgggatg gcagaggtcg tatccaaggc cgtgtacaac acacgagaga
     1201 ttggcggttc ggacggcatc attggaatcc ctgtggttca tatcgatctt ttattttttt
     1261 ctgtcgatag caataacgcg ctttcgtttt ttttgctggt gctggccata atcatggggt
     1321 tgtattttct cctggaggcc atgctgcaaa ccccttttgg gcggcttgta aaggcgatca
     1381 gggccaacga aggccgcgtg cctttccttg gcttcagttc gcaacacacc aagttgtatg
     1441 tattcatatt ggcgtccaat gtggcggccc tttccggcgc cctctatcca atgctgcgcg
     1501 gcttcgtttc acctgagcta atgttttttc acacatccgc cgatgcggtg attactgtga
     1561 tactgggagg cgttggcacg ttaatcggcc cgctatatgg aagcgtcatt ttgacgacat
     1621 tgaaatccat tataggcaca tatacggaac accagcagat tgtggtggga gcgttattca
     1681 tgctgtcggt tatttttttc cctggcggct tggcggggta tttcaggccg cgccttaacg
     1741 cttggctcac acggcgcgag gggcggtcat gagttccgcc aatccgggag gcgctcggat
     1801 tccagagcag ccgtccattc tggaagtgaa gaatgtttcg gtgcgattcg gcgcgttggt
     1861 tgctgtgaac gccgtaagtt tcgttgcgcg gcggggcagc gtcacttcgg tgatcggccc
     1921 caacggcgcc gggaaaagca cgttatttaa cttaatcagt ggcgccttga cttcaagcag
     1981 cggcgaggtg tggtttgatg gagccaacgt gactggttgg gccgccagca ggctggtgcg
     2041 aaaaggatta tcgcgctctt ttcagattac gaatctgttt tttgaaattt ctgtttatga
     2101 gaatttgcgt cttgccgcgc aggtgcttga accgcagtct aaattgttga tgccattgcg
     2161 tagaagcgcg cttgcgcgcc agcggacgga agagctgatt gaacagttta aattggatga
     2221 caaagtacat gagcttgcgg gttacctttc tcatggcgag cagcgacgcc tggaaatcgc
     2281 ggttgcgctt gcaagcaggc caaaggtgtt gttgcttgac gagcccactc agggtatgtc
     2341 gcacgccgac acagaggaaa ccagcaaatt gatcaggagt ctttctcgcg atgtttcaat
     2401 tctgctggtc gagcacgacg tgagccttgt aatggattta tccgaccatg ttgttgtgat
     2461 ggcgcagggg gcgaaattgg cggatggcac acccgcgcaa attcgatcca atctggaggt
     2521 acaagcggct tattttggac gggaagtgtc acatgcttga gcttgatgga ttgcacactc
     2581 attatgggct aagccatgtg ctgcagggca ttagcctgac catcgaagcg ggagaggtgg
     2641 ttttgttgtg cggccgtaac ggagtgggca aaaccacggt aatcaagaca atagccggtt
     2701 gggttgcgcc ttcttcagga tctgtccgct ttgatggcat tgaaataggt ggattgacgc
     2761 cggatcgaat ttgtcgtttg ggcattggct tggtgccgga agatcggcga attttcccgg
     2821 gcttgacggt ggaagaaaat atcaagctgg gctttttgca atgtcccggg cgcgctgcgg
     2881 ctcagtcccg gcgagcgctg gaagaaacct atgaacggtt tccgcgtctt agagagcgcc
     2941 gcgagcaaat gggcactact ttatcgggtg gtgagcagca aatgttggcc attgcccggg
     3001 tgttggtggg tgcgcccaaa tttctgctga tagacgaacc cactgagggc ttggcgccaa
     3061 tgatcgtgga tgagattttt accatgattg atagcttgcg tagcgaggga attactgtcc
     3121 tgatggtcga gcaaaacgtg catcgcgccc ttgatgtttc aacccgattt tacgcattgg
     3181 aacgtgggca aatcatcgtc caggggagcg cctcgtcgcg tgccgacaga gatgatttga
     3241 tgaaagcaat tactgtttga tggaccgtcc gaacgacgtt ttgagataac agctttccgc
     3301 tgtgagcggc tgccgcaact gttcaaattt gcctgtattt aaggggaaaa tcgagatgat
     3361 tgagtcatta aattccgctg cgccactgac tttacgccgt tggctgcgtc gcctcgcaag
     3421 tacgaatcgt ctggttgtcg cccgcccgga tgttggcttg aaatatcagt tggccgctat
     3481 tgcaaaacgt cttgatggca gcaaagccgt cctatttcct cgcccggatg gtcatggcat
     3541 cccgatagtt tccggtttta tggcgcggcg cga
//
LOCUS       DATCXU010000175         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1036915,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000175 DATCXU010000000
VERSION     DATCXU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036915"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VIR04_03910"
     CDS             <1..1265
                     /locus_tag="VIR04_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY9459925.1"
                     /translation="WLCGLSWSFGSLVVFRVMQGAVAGPMIPLSQSLMLSSYPKEKAG
                     MALAMWSITVLVGPIAGPLLGGWISDNYSWSWIFFINVPVGLLAAWGSWQLYKERDSA
                     RVKLPIDRIGLALLIVWVGALQIMLDKGEDLDWFSSTFIITLAVIAVVLFVFFVIWEL
                     TAEHPVVDLTLFKERNFTSGTVTISVAYGVFFGTVVLLPLWMQNTLGYTATDAGIASA
                     PVGIMAIILSPIVGKLLAKHDARYIVTLGFIIFGVTSYMRALFNTDVDIVTIMWPTFI
                     QGAAMAMFFIPLTTISLSGLDPHRIPSAAGLTNFARLLCGSFGTSITTSLWDNRAILH
                     HAQLTEIVTSGAPAFDQAMSGLQKKGLTHEQSASLINNLINQQAFTLSATDIFYASAI
                     LFIALIALVWVARPKLAGSGSGAAAGAH"
     gene            1365..1440
                     /locus_tag="VIR04_03915"
     tRNA            1365..1440
                     /locus_tag="VIR04_03915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1398..1400,aa:Asn,seq:gtt)
     gene            1972..2544
                     /locus_tag="VIR04_03920"
     CDS             1972..2544
                     /locus_tag="VIR04_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459926.1"
                     /translation="MSYLKTPALAKSAVLAAAFALPCLATAAPSVATPNTAQNVAPAS
                     AGQNKTDVPHAKKRQHKNKSVAMWVPGFGPVNQKTVTSLKLNDSQQKLVAEAKAAQDD
                     FQKNLHDLEQATRQGQKTQLDTGKIDPHAAQNAKDDIWKKGMGERRDQLTKKWLAVWD
                     ALDSTQQQKVTAYLKQKADKPGSSNWNERR"
     gene            2843..3622
                     /locus_tag="VIR04_03925"
     CDS             2843..3622
                     /locus_tag="VIR04_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEY9459927.1"
                     /translation="MHRANFLWLDFRRNTSASGLFHSLAPSYNAHILPHPCDPSPDID
                     AQRPHFLCFEFDTPLSSDLAVLRRTRLQHPDLPLLMITEGHSESLAIWAFRSGVWDYL
                     VKPLATGDLDTCIASLCRFCLEHRRTGPLTPWPLPSSMFAQSSGSASRVNKTLSAREF
                     VKAHFAETIRLSVVATHCHMSESEFSRFFKKEHGDTFYEYLLKFRIGKACELLADASV
                     QVKSVAFAVGFNDLSYFARTFRRYTGVTPSTYQQACAAHGR"
     gene            3802..>4031
                     /locus_tag="VIR04_03930"
     CDS             3802..>4031
                     /locus_tag="VIR04_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459928.1"
                     /translation="MATKEKETGIGSSRGAFILEGSIALQESEGEDQDLGVKVFAFDR
                     LGEPLGGGEPDGKGNFRLTLRLAAPADIQIMAG"
BASE COUNT          888 a         1142 c         1081 g          920 t
ORIGIN      
        1 cctggctatg cggactgtcc tggtcgtttg ggtcgctggt ggtctttcgt gttatgcaag
       61 gcgcggtggc aggcccgatg atccccctgt ctcaatcgct gatgctgagc tcctatccaa
      121 aagaaaaagc cggcatggcg ctagcgatgt ggtcgatcac agtgttggtt ggccccattg
      181 cggggccctt gctgggaggg tggatttccg acaactactc ctggtcatgg atatttttta
      241 tcaatgttcc tgtcgggctg cttgcggctt ggggctcctg gcaactgtac aaggagcggg
      301 actcggcccg cgtcaaactg cctatcgaca gaatcggtct ggcgcttttg atcgtatggg
      361 tgggcgcgct gcaaatcatg ctggacaaag gtgaagacct ggactggttc agcagcacgt
      421 tcattatcac tttggccgtt atcgccgtcg ttttgttcgt gtttttcgtg atttgggaac
      481 tgaccgcgga acaccccgtg gtggacctca ccttgtttaa agagcgcaac ttcacctccg
      541 gcaccgtgac gatctcggtt gcctacggcg tgttcttcgg cacggtggtt ttgctgccgc
      601 tatggatgca aaatacgctg ggctacacgg cgaccgacgc gggtattgcc tccgcgcccg
      661 tcggcattat ggcgattatt ctttcgccaa tcgtcggcaa gcttctggcc aagcacgatg
      721 cgcgctatat cgtcacgctg gggttcatta ttttcggcgt caccagttat atgcgcgcgc
      781 tctttaacac cgacgtcgac atcgtcacga ttatgtggcc gaccttcata cagggcgcgg
      841 ccatggccat gttcttcatc cccctgacca cgatttcgct atcgggcctg gacccgcacc
      901 gcattccctc ggccgccggc ctgacgaact ttgcgcggct gttgtgcggt tcgttcggca
      961 catcgatcac caccagtctt tgggacaatc gggccatcct gcatcatgcc caattaacgg
     1021 aaattgtcac ctcgggagcg cccgcgttcg atcaagccat gtccggcctg cagaagaagg
     1081 gactcaccca cgagcagtcc gcctcgctca tcaacaatct aatcaaccag caagcgttca
     1141 ccctgtccgc caccgacatt ttttacgcct cggccattct gttcatcgca ctcatcgcgc
     1201 tggtatgggt cgcacgcccc aagctagcgg ggtccggatc gggcgccgcc gccggagcgc
     1261 attaagtgcg ggggctgagc cgatccggct cataccgaca ctgtttgtgg tggtattctg
     1321 ccggtgcttt tcaatgattt atgctatagt cttgcttctt taattccccg atagctcagt
     1381 tggtagagcg acggactgtt aatccgcagg tcactggttc gagcccagtt cggggagcca
     1441 agaaattcaa gcctttcgag agatcgaagg gcttttgttt ttgtgccata tgtgacgttt
     1501 tatgagacgc gtcatatatc cagtagtttt gcgcggcctg ttcaacgccc tacgcgctgc
     1561 gcgcaaaaag gagacggcgc aacgataact agcaattccc tttcttcgct tgccccggtg
     1621 ggcagaatcc accacctcga ttgtcgtgcc gatcgtagta gccatcgttg cgatggccac
     1681 ctcggtcatg cacgtatcca atacacccgc tcaatacgga cgccatcaac gtgaccagta
     1741 caactcgcag cattcttacc tctttcgttt ttccgacaca ctgattctag cggcccgacg
     1801 gtgctctcgg gtggcaaata gtaacgaagc gcttcgcgcg ccatgccaat aaaaccaata
     1861 aaaacaattt acggcgctcg aaacattcgg ttaagatccg tttcaaggct tgctgcgcgc
     1921 acgcacaaca tgctgacact caatttctgg cccatataac ggataactaa aatgtcatat
     1981 ttgaaaacac ccgctctggc taaatccgct gtactggcgg cggcgtttgc actaccctgc
     2041 ctggcaacag cggccccctc cgttgcaacc ccaaacaccg cacaaaacgt tgcgccagcg
     2101 agcgccggac aaaacaagac tgacgtacct catgcgaaaa agcgtcaaca caaaaacaag
     2161 agcgtggcca tgtgggtgcc tggctttggc cccgtcaatc aaaagacggt aacctcactg
     2221 aagctgaacg atagccaaca aaagttggtt gcggaggcga aggcagcgca agacgatttt
     2281 caaaaaaact tgcatgactt ggaacaggca accagacaag ggcaaaagac gcaattggac
     2341 acggggaaaa tcgacccgca tgccgctcaa aacgctaaag atgacatctg gaagaaaggg
     2401 atgggcgagc gtcgcgacca gctcaccaaa aaatggctgg ccgtctggga cgcgctggat
     2461 agcacgcagc aacagaaggt caccgcttac ttaaagcaaa aagctgacaa accgggctca
     2521 tccaactgga acgagagacg ctagccgtag gcaaacagcc ggcacttaaa aacgaaaaaa
     2581 acggtggcct gctttcagaa tggccgccgt tttttttgaa cagagggaca tgcgattttt
     2641 ttatatttgc accgctccca attgcttaac cctgacggtt ccgtccgatt gcaaagtgtg
     2701 taacgaattc ggtcttcctg cacgaaatcg atagcagtgg acatactatg gcaataattt
     2761 tgttgggatg gccgaagggc gcgtccgcga gaaccggacc gctcccggcg tccgtggcgc
     2821 gggaagcgaa caggagaagc cggtgcaccg agcgaatttt ctgtggctgg attttcgacg
     2881 gaacacgtcg gcttcgggac tgttccattc gctggcgcct tcatacaacg cgcacatatt
     2941 gcctcacccc tgtgacccca gccccgacat tgacgcgcag cggccccact tcctttgctt
     3001 tgagtttgac acgccgcttt caagcgacct ggctgttttg cgccgaacca ggctgcaaca
     3061 cccggatctg ccgctgctga tgatcacgga aggccattcg gagtcactcg ccatctgggc
     3121 ctttcggtcc ggagtatggg actacctggt caaacccttg gccacgggtg atctggatac
     3181 gtgcattgcg tctctgtgcc ggttctgtct tgaacacagg cgcaccggtc cgttgacacc
     3241 atggcccctg ccaagctcca tgtttgcaca gtcgtcgggc agcgcatcac gggtgaacaa
     3301 gaccctttca gcgcgtgagt tcgtaaaagc gcatttcgcg gaaacaatac gtctgtcggt
     3361 cgttgccacg cattgccata tgagcgaatc ggaattcagc cggttcttca agaaagagca
     3421 tggcgacacg ttttatgagt atctgctgaa attccgtatc ggcaaagcct gcgaattgct
     3481 ggccgatgca tcggttcaag tcaagtcggt cgcctttgcc gtgggcttta acgacctttc
     3541 ctattttgcc cgcacctttc gacgctacac gggcgtgacg ccttcgacct atcaacaagc
     3601 gtgcgctgcg cacggccggt gaatgcattt gccctggcag agcatttacg caagatagtt
     3661 ttagtgttgc gcgacatcct cctaactccc taagccttaa tttttttata ttgcaaaccg
     3721 tataccggtt gcaatcgacc ggcagcgcaa gcagtagaaa gtgtccttgc atgcaaaacg
     3781 cccactcagg agttcaccgt catggccacc aaagaaaaag aaacaggaat cgggagctcg
     3841 cgcggcgcat tcatactcga aggcagcatt gccttgcagg agtcggaagg cgaggatcag
     3901 gatctggggg tcaaagtctt tgcctttgat cggctcggtg agccgctagg cggcggtgaa
     3961 ccggatggca agggcaattt caggctgaca ttgcgcctgg ctgcgccggc ggatattcag
     4021 atcatggcag g
//
LOCUS       DATCXU010000176         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_295615,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000176 DATCXU010000000
VERSION     DATCXU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295615"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VIR04_03935"
     CDS             <1..1030
                     /locus_tag="VIR04_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY9459929.1"
                     /translation="ARRLGGAGLASAARQRMRLAGMNEAQIRSVVSSGKVQPRSTVVS
                     PIDGVATELSVREGMTVSMGAPLFRINGLSTVWVNAEVPENLADQIQIGNVVQAQTRS
                     LPGTTFHGKVSAILPDVNATTRTLKARIELSNPDRQLVPGMFVRVNFVAPTREDVLLV
                     PTEAVIQTGTRSVVIVVQDDGKFAPVDVTIGTEAHGQTEIRKGLKAGQKIVSSGQFLI
                     DSEASLKGSTTRMADPTSPPASPPPASQATEPVHHGVGTIKDIGKDAITIAHGPIPTL
                     KWGAMTMGFALPAGGLPKNIAVGDSVAFDIRPMADGMFQIVKISPATVSSGNAKASEG
                     KSESGAAQ"
     gene            1027..>2210
                     /locus_tag="VIR04_03940"
     CDS             1027..>2210
                     /locus_tag="VIR04_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9459930.1"
                     /translation="MIAKLIRWSISNRFLVLLATLMIIAWGVLSVSRMPLDALPDLSD
                     VQVIVRTSYPGQAPQIVENQVTYPMTTTMLSVPGAKVVRGYSFFGDSFVYILFEDGTD
                     PYWARSRVLEYLSQVQSRLPPQAKASLGPDATGVGWIYEYALTDKTGKMDLSQLRALQ
                     DWFLKYELKTVPNVSEVASIGGMVRQYQIVLDPEKLRAYNIPQEKVIDAVQKANQETG
                     GSVLELGEADYMVRSSGYLKSLDDFRKIPLATTDAGVSVRLGDVARIQIGPEMRRGVA
                     ELNGEGEVAGGVIIMRSGKNALETINAVKAKLQTLKASLPPGVEIVPTYDRSNLINRA
                     VTNLQEKLIEEFIVVAIVCALFLFHLRSSLVAIITLPIGILIAFIVMVYQGVNANIMS
                     LGG"
BASE COUNT          509 a          602 c          643 g          456 t
ORIGIN      
        1 agccagacgt ttggggggcg cgggcttggc ctccgccgcg cgacaaagga tgcggctagc
       61 gggtatgaat gaagcgcaaa ttcgatcagt ggtttccagc ggcaaagtgc aaccacgctc
      121 caccgtggtc agtccgattg atggcgtggc gactgaactg tcggtgcgcg aaggcatgac
      181 cgtatcgatg ggtgcgccgt tatttcgcat taatggccta agcacagtct gggttaacgc
      241 cgaggtcccc gaaaatctgg ctgatcaaat acaaatcggc aacgtggtgc aagcgcagac
      301 tcgttcgttg ccgggaacaa ccttccacgg caaggtgagc gcgattttgc ccgacgttaa
      361 tgcaacaaca cggaccctga aggcgcgcat tgagctttcc aatcccgaca ggcagcttgt
      421 tccgggaatg ttcgttaggg tgaatttcgt cgcgccaacg cgcgaggacg tcctattggt
      481 gcccaccgag gcggtcattc aaacgggtac ccgcagtgtc gtgatcgtcg tccaagacga
      541 cggcaaattc gcacctgttg atgtgacaat cggaacagag gcccacgggc aaactgaaat
      601 tcgcaagggt ctgaaggccg ggcagaaaat agtaagttca ggccagttcc tgatcgactc
      661 cgaagccagt ctgaaaggca gcacgacgcg tatggccgat ccgacctccc caccagcatc
      721 accaccgccg gcgagccaag caaccgaacc ggtgcatcac ggcgtaggca caataaaaga
      781 catcggaaag gatgcgatta cgatcgcaca cggaccgata ccgacactga aatggggtgc
      841 gatgaccatg ggctttgcac ttcccgctgg cggcctaccg aagaatatcg ccgttggcga
      901 ttccgtcgcg ttcgacattc gtccgatggc tgacggcatg ttccaaattg tcaagatttc
      961 gccagcaacc gtgtcgtcgg gcaatgcgaa ggccagtgaa ggcaaatccg aaagcggagc
     1021 ggcccaatga tcgccaaact aattcgctgg tcgatcagca accgattcct ggtgctgctc
     1081 gccaccctta tgatcatcgc ctggggcgtt ttgtcggtat cgcgcatgcc cctggacgcg
     1141 cttcccgatc tatccgacgt gcaggtcatc gtgcgcacca gctatccggg ccaggcgccg
     1201 caaatcgttg aaaatcaggt cacctacccg atgacaacga ccatgctctc ggtgccaggc
     1261 gccaaggtgg tgcgcggtta ttcttttttt ggcgactcgt ttgtctacat tctgtttgag
     1321 gacggcaccg acccttactg ggcgcgctcc cgcgtgctgg aatatttaag ccaggtgcag
     1381 tcgcgcctgc cgcctcaggc gaaggcttca ttgggcccgg acgccacggg cgtgggatgg
     1441 atctatgagt acgcgttgac cgataaaacc ggaaagatgg atttatcgca attacgcgct
     1501 ttgcaagact ggttcttaaa gtacgaactt aaaaccgttc caaatgtatc cgaagtagcc
     1561 agcatcggcg gcatggtgcg gcaataccaa atcgtgctcg atcccgagaa acttcgcgct
     1621 tacaacattc cacaagaaaa agtgattgac gccgtacaaa aggccaatca agaaacagga
     1681 gggtcggtgt tggagctggg cgaagccgac tatatggtgc gttcgtcggg ctacctgaaa
     1741 tcgctcgacg atttccgcaa gattccgctg gccacgacgg acgccggcgt gtcggtgcgc
     1801 ctgggcgacg tcgcgcgtat tcaaataggt cccgagatgc ggcgcggcgt cgctgaactt
     1861 aacggagagg gagaagtagc gggcggcgtg attatcatgc gttcgggaaa aaacgcgctt
     1921 gaaacgatca atgccgtgaa ggcaaagttg cagacgctga aagcaagcct gccgcccggc
     1981 gttgaaatcg tcccgacgta cgatcgctcg aatctgatta atcgagcagt caccaatctg
     2041 caagaaaaac tgattgaaga attcattgtc gtggcgattg tctgcgccct gttcctgttt
     2101 cacttgcgct cgtcgctggt tgcgatcatc acgctgccca tcggtatatt gattgcgttc
     2161 atcgtgatgg tttaccaggg ggtcaatgcg aacattatgt ccctgggagg
//
LOCUS       DATCXU010000177         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_318448,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000177 DATCXU010000000
VERSION     DATCXU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318448"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VIR04_03945"
     CDS             <1..556
                     /locus_tag="VIR04_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY9459931.1"
                     /translation="LTASVMLVLAGGKCFPVQEAAQDAASEGNAQASATPRRRWYDKP
                     TLPPAPASDDVQPGNLTASRSSAGSVGRQFAADNARDAAIRPKEAQHLSPSVIANEAE
                     MLGVTPRQFEVLALLAKGCSIKGISRELNISIATTKVHAQTLYQRLGVHNRNAAIHTA
                     FSHGATLGWSGLDVDVSGGNVVKS"
     gene            complement(622..1431)
                     /locus_tag="VIR04_03950"
     CDS             complement(622..1431)
                     /locus_tag="VIR04_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEY9459932.1"
                     /translation="MDIPFNSADQEALRNAHRLLENPGLAARITDLIGSPIEQGLNLL
                     PESWGKKISSVTKTALTKAADAAIFTLEDKPGDESSNFWHKFGVAATGGVGGFFGLAG
                     LTIELPISTTIMMRSIADIARSEGESLSSPDTRLACLEVFALGGKSVSDDGTDSGYYV
                     VRAALTHAVSQAAEYAAGSGLAEKAAPPALMRLITLIAERFGTQITEKAAAQAVPAIG
                     AVGGALINTLFIDHFQDMARGHFTIRRLERRYGKEVVQLAYKALSQDSNGA"
     gene            complement(1466..>3993)
                     /locus_tag="VIR04_03955"
     CDS             complement(1466..>3993)
                     /locus_tag="VIR04_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEY9459933.1"
                     /translation="LPPRNPLDVFFDIEGFPLDEGGLEYLWGCTYFDGSGKRQFKDFW
                     AHDREQEKQAFGSFIAWAYARWQQEPGMHIYHYANYEIAACRRLMGRYGICEHQVDQL
                     LRNEVFVDLYKIVKGGLRVGEPRYSIKNIEHLYRGKRDTIVGNGGDSVAVYELWRNRH
                     EEGLEGDTWQTSEILKNIRVYNIDDCNSTQELATWLRVQQQEHHMDYVGTKDIVEPEL
                     GEEVTERTKLRDRLLARAASEPEGQARITENLAWMLEFHRRESKPTFWRLFDRLGLSH
                     TELEDDIDCLANCRRTRREPFKATERARTYAYEYRFNPDQEFKLPRQAGMYLLGDSRQ
                     KVTFVSEESNLVKGLVVVKSKDNPGDLISLIPDEYVNPGAIVDAINDVVARYEAGTLA
                     DSAILAFLRREPPRIKDHRAGPIATAADPAGRLVEIIHAVQSLDRSYLTLQGPPGAGK
                     TYTAQHIIAALVKQGKRVGISSNSHKAINNLLLGVAKTCLSEHISVDCYCTKDTGPEI
                     SKNHITVTKNEEILGTVAPGCVIGTTAWGFCRPEMISQLDYLFIDEAGQVSVANLVGM
                     SRAAQNLVLMGDQMQLGQPSQGSHPADSGLSILDYLLHDSPIIPDTMGVFLDTTFRMH
                     SAVNDYVSHAIYRGELKADAANDKQRVCVPAGYCGVLHAEAGVVFVPVDHQGNTQASN
                     EEVDVITALAKDCLGRTFIGKNGTERSITWKDMLFVAPYNHQVNRLQQALGADARVGS
                     VDKFQGQEAPIVFFSLCTSDASESPRGIDFLFDKHRINVAISRAQAMAIVVGNPGLFC
                     TEVGNVAQMSQVNVLARLLHARSVTAPEIALSPDAVAEMNLAQ"
BASE COUNT          822 a         1169 c         1092 g          910 t
ORIGIN      
        1 actcaccgca tccgtcatgc tcgttctggc cggtggaaag tgctttccag tacaggaagc
       61 ggcgcaagat gcagcgagtg aaggcaacgc ccaagccagc gctacgccac gacgccggtg
      121 gtacgacaag ccaactcttc cacccgcgcc ggcctctgat gatgtgcaac cgggcaacct
      181 gacagcgtca cgtagcagcg ctgggtctgt aggccggcag ttcgccgccg acaacgcacg
      241 tgatgctgcg atcagaccaa aagaagctca gcacttatcg ccgtccgtga ttgccaacga
      301 ggccgagatg cttggcgtaa cgccgcgtca gtttgaagtg ttggccttgc tcgccaaagg
      361 ctgctctata aagggcatca gtcgcgaatt gaacatttca atcgcgacga cgaaggtgca
      421 cgcccagacg ctgtatcagc gcctgggcgt gcacaaccgc aatgcggcga tacacaccgc
      481 tttttcgcac ggtgcaacgc tgggttggag cggattggat gtcgacgtct ctggcggtaa
      541 cgtggttaag tcttgaaagg ggctgggaaa gtcgcactca actacgggtc accacacaga
      601 aagccgtgca ccatatctaa gctaggcgcc gttgctgtct tggctcagtg ctttataggc
      661 taactgcact acttcctttc catagcggcg ttccagccgc cggatagtga aatggcctcg
      721 ggccatgtcc tggaaatggt cgatgaacaa tgtattgatc aaggcgccgc ctactgcacc
      781 gatggcggga actgcctgtg ctgctgcctt ttccgtgatc tgagtgccga agcgctcggc
      841 gatcagagtg atcaatcgca tcagcgcggg tggcgcggct ttttcagcca gtccacttcc
      901 agcagcgtac tcagccgctt ggctgacagc atgagtcagg gccgcccgca caacgtaata
      961 gccgctatcc gttccatcat ccgacacgct cttgccgccc agggcaaaca cctccaggca
     1021 agccagcctg gtatcgggac tgcttagtga ttcgccttca ctacgggcga tgtcggcaat
     1081 tgagcgcatc atgatcgtgg tggaaattgg cagttcaatc gtcagacctg ccagtccgaa
     1141 aaagccgccc acgccgcctg tcgcggcgac gccgaattta tgccagaagt tcgagctttc
     1201 atcgcccggc ttgtcctcta gagtaaagat ggcagcgtcc gctgccttgg tcagcgccgt
     1261 tttggtgaca ctgctgattt ttttgcccca gctctccggt aacagattca aaccctgttc
     1321 gataggtgaa ccaatcagat cggtgatgcg cgccgccaag ccgggatttt ccagcaggcg
     1381 atgggcgttg cgtagcgcct cctggtcggc cgagttgaag gggatatcca ttaggaaggc
     1441 tccatccttt ctgccatggg gtctgttatt gcgcaaggtt catttctgcg accgcgtccg
     1501 ggcttaaggc aatttctggc gcggtgacag aacgcgcatg cagcaagcga gccagcacgt
     1561 tgacctgact catctgcgcc acgttgccga cttcggtgca aaatagtcct ggattgccca
     1621 ctacgatcgc catcgcctga gcgcgtgaaa tggccacgtt gatccgatgc ttgtcaaaca
     1681 ggaaatcaat gccgcgcggc gattccgagg cgtcgctggt gcaaaggctg aaaaatacaa
     1741 tcggcgcttc ctggccctgg aatttgtcca cgctgcccac tcttgcgtcg gctcccagcg
     1801 cctgttgcaa ccggttcacc tgatggttgt atggtgcgac gaacagcatg tctttccacg
     1861 tgatgctccg ctccgtgcca tttttgccaa tgaaagttcg ccccaggcag tcctttgcca
     1921 acgccgtgat gacgtcgacc tcttcattgc ttgcctgggt gttgccctga tgatccaccg
     1981 gcacgaacac cactccggct tctgcgtgca gcacaccaca atacccggct ggcacgcaca
     2041 cccgttgctt gtcgttggcg gcatctgcct taagctcgcc gcgatagatg gcgtgactga
     2101 cgtagtcatt gacggcggag tgcatacgaa acgtggtatc cagaaacacg cccatggtgt
     2161 cgggaatgat aggcgagtcg tgcagcagat agtcaagaat cgacaagccg ctatcggccg
     2221 ggtgcgatcc ctgcgatggc tgacccaact gcatctggtc acccatcaaa acaagattct
     2281 gcgccgcacg gctcatgcca accaggtttg ccaccgatac ctggcctgcc tcgtcgatga
     2341 acaggtaatc cagttgactg atcatttccg ggcggcaaaa tccccatgcg gtggtcccga
     2401 ttacgcatcc gggcgccacg gtcccaagga tctcctcatt tttcgtaacc gtaatatggt
     2461 ttttagagat ctccggaccc gtgtctttgg tgcaataaca gtccacactg atgtgttcgc
     2521 ttaaacacgt tttagccacg cccagcaaca ggttgttaat ggccttatgg ctattgctgc
     2581 tgatgccgac cctttttccc tgtttgacca gtgcagcaat aatatgttga gccgtatagg
     2641 ttttgcccgc gcccggcggc ccttgcaatg taaggtagct gcggtccaga ctttgtaccg
     2701 cgtgaatgat ttccacaaga cgcccggcgg ggtcggccgc cgtggcgata ggcccggccc
     2761 gatggtcctt tatccgcggc ggttcccggc gcaaaaaggc cagaatggcc gaatcggcga
     2821 gtgtaccggc ttcgtagcgg gcgaccacat cattgatcgc gtcaacgatg gcccctggat
     2881 tgacgtactc gtcggggatc agcgaaataa ggtctcccgg attgtccttg gactttacga
     2941 ctacaagccc ttttaccagg ttcgactctt cggacacgaa cgtaaccttc tgccgggaat
     3001 caccaagcag atacatgcct gcctgacgtg gcaatttgaa ttcctgatcg ggattgaacc
     3061 ggtattcgta ggcgtaagtt cttgctcgtt cggtggcctt aaagggctcg cgcctggttc
     3121 ttcggcaatt ggccagacaa tcaatgtcgt cttcgagttc ggtgtggctt aatcccaggc
     3181 gatcgaacaa acgccagaat gtgggcttgc tttcacgccg atggaattca agcatccagg
     3241 ccaggttttc ggtgatccgg gcttgcccct cgggctcgct tgcggcgcgc gccaatagcc
     3301 gatcgcgcag cttggtccgc tcggtgacct cttcaccgag ttcgggctca acaatgtcct
     3361 tcgtcccgac ataatccata tggtgctcct gctgttgcac gcgcaaccac gtggccaatt
     3421 cctgagtgga attgcaatca tcgatgttgt agacgcggat gttcttcagt atctctgatg
     3481 tttgccaggt gtcaccttcc aggccttctt cgtgacgatt gcgccataac tcgtacaccg
     3541 caaccgagtc cccgccattt ccgacgatcg tatcgcgttt gccgcgatac aagtgctcga
     3601 tatttttgat cgaatagcgc ggctctccaa ccctaaggcc gcctttgacg atcttgtaca
     3661 agtctacaaa gacttcgttg cgcaagagtt ggtcgacttg gtgttcgcaa attccgtagc
     3721 ggcccatcag gcgccggcag gcggcgatct cgtaattggc gtaatggtaa atatgcatgc
     3781 caggctcttg ctgccagcgc gcataagccc atgcgataaa tgaaccaaag gcctgctttt
     3841 cctgctcgcg gtcgtgagcc cagaaatcct tgaattgacg ttttccggag ccgtcaaagt
     3901 aggtgcatcc ccacaggtat tccaggccgc cctcatccaa cggaaagcct tcaatgtcaa
     3961 agaacacatc cagcggatta cgcggtggca ata
//
LOCUS       DATCXU010000178         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_487698,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000178 DATCXU010000000
VERSION     DATCXU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487698"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VIR04_03960"
     CDS             complement(<1..1872)
                     /locus_tag="VIR04_03960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY9459934.1"
                     /translation="MNSNPLLAPIDSLIDYAAIEPEHIEPAVSGLIRDARAAVEKAAD
                     PHTGTSWASFIEPLEDASERLWRAWSVAGHLNAVVNTPALREAYNQCLPLITEFSTWT
                     GLHTGLYAQYKRLKADPAFAGLSPTRQRIVDLALRDFRLSGVELQGKQRERYAEISDQ
                     EAQASQKFSENMLDSIDNWSLHIQDENRLAGLPRDVRQAAQAAAQEAGLDGWKLTLKM
                     PCYLPVMQYAQDRSLRETLYRAHATVASEQGDPSLDNSPVIEQLLALRAEEAAMLGYK
                     SFADLRLQSRMANSAREVLDFLGKLAVRAKPYAIHDLAELRRFARENLSLDLLEPWDI
                     PFASERLREQRYAYSEEEVKQYFTEPQVLKGLFHVIDTLFNVKLRQSIAKTWHKDARV
                     LLIEAPDGHLLGHLYIDLYARQGKQGGAWVDSERSRRKAGDHVQTPIAYLTCNFSAPQ
                     EGKPALLTHDDVITLFHESGHALHALLSEVDDPGASAFASVEWDAIEMPSQFMENFCW
                     EWSVVQSLSANVDNGAALPRALYDKMLAAKNFQSGLQTVRQIEFALFDMTIHDQSKGL
                     SIQSVLQTLDQVRRKVAVLFPPSWHRFPHNFSHLFAGGYGAGYYSYKWAEVLSADAYA
                     "
     gene            complement(1888..2751)
                     /gene="folD"
                     /locus_tag="VIR04_03965"
     CDS             complement(1888..2751)
                     /gene="folD"
                     /locus_tag="VIR04_03965"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY9459935.1"
                     /translation="MTARIIDGKALSAAIKADVAKRVLDLKKKGITPGLAVVLVGDNP
                     ASAVYVRNKAAACEKAGLASKVIRLESSVSEEQLLDVVKKLNDDASVHGILVQLPLPP
                     HIRPGKVIETIAVEKDVDGFHVSNAGLLMTGRPSIRPCTPYGIMKMLQAEGATVWGAE
                     AVIVGASNIVGKPMAMLLLAAGATVTLCNSKTRDLAAQTRRADILVVATGKPGIVKGD
                     MIKPGAIVIDVGINRNADGKLMGDVEFESAKKVAGAITPVPGGVGPMTIAMLLVNTLE
                     AAERNAAHQDL"
     gene            complement(2748..3377)
                     /locus_tag="VIR04_03970"
     CDS             complement(2748..3377)
                     /locus_tag="VIR04_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY9459936.1"
                     /translation="MTTHQTSSSTIFIVDDDEAVRDSLRWLLEANGYRVKSFGGAEEF
                     LNAYDPDQVGVLIADVRMPGMSGLELQEELIARKAPLPIVFITGHGDVPMAVSTMKKG
                     AVDFLEKPFNETDLRTIVARMLEQACERVTQNQVLKSHQAVLSRLTTREQQVLERIVA
                     GRLNKQIAGDLNISIKTVEAHRANIMEKLEVTTVADLMKIALNKAQGAA"
     gene            complement(3374..>3893)
                     /locus_tag="VIR04_03975"
     CDS             complement(3374..>3893)
                     /locus_tag="VIR04_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9459937.1"
                     /translation="REFVKRSEPRRQRVVIKRILDNAIGFAEIDARKRQVTIEQDLPD
                     SLPDVVADPILIEQVLLNLLKNGIEAMEKSEYRVLHVVVTGQSPLVEIAVIDRGYGLR
                     DPERLFEPFYSTKSEGLGMGLNICRTIIESHHGRLWASTNPDGGTIFRFTLPCATPEE
                     PASEESPQELHA"
BASE COUNT          793 a         1083 c         1109 g          908 t
ORIGIN      
        1 ggcataggcg tctgccgata atacttcagc ccatttatag ctgtaatagc cggcaccgta
       61 gccacccgcg aacaggtgtg aaaaattgtg aggaaaacga tgccaggacg gtgggaacag
      121 cacagcaact ttgcggcgca cctgatcaag tgtctggagc accgactgga ttgaaagccc
      181 tttgctctga tcgtggatgg tcatgtcgaa cagggcaaat tcaatttgtc tgacggtttg
      241 cagtccgctc tggaaatttt ttgcggccag cattttgtcg taaagcgcac gtggcagtgc
      301 ggcgccattg tcaacattcg cggatagcga ctggacgact gaccattccc agcaaaaatt
      361 ttccatgaac tgcgatggca tttcgatggc gtcccattcc acgctggcaa acgccgacgc
      421 acccgggtca tcgacttcag aaagcagggc gtgcagcgca tgtccgcttt cgtggaaaag
      481 tgtgatgacg tcgtcatgcg tcaacagcgc gggcttgcct tcttgcggcg ccgagaaatt
      541 gcaggtcagg taggcaatgg gcgtttgaac gtggtcgcct gctttgcgcc ggctgcgttc
      601 gctgtcgacc caggctccgc cttgcttgcc ctggcgcgcg tacaaatcga tatacaaatg
      661 gcccagcagg tgtccatccg gcgcctcgat cagcaaaaca cgggcatctt tgtgccaggt
      721 ttttgcgatg gattggcgca gtttcacgtt aaaaagggta tcgatcacat ggaacagacc
      781 ctttaatacc tgcggctcgg taaaatattg ttttacttct tcttccgagt aggcgtagcg
      841 ttgttcacgc agtcgttccg aagcgaaggg aatatcccag ggttcaagca agtccagcga
      901 caggttttcg cgcgcgaacc ggcgtagttc ggccaggtcg tgaatggcgt aaggcttggc
      961 ccttacggcc aatttgccca gaaaatccag aacttcgcgg gcgctattgg ccatgcggga
     1021 ttgaagccgc agatccgcaa agcttttgta gcccagcatg gcggcttctt cggcgcgcag
     1081 ggccagcagt tgttcaataa caggcgagtt atccagactg gggtcgccct gttcggaagc
     1141 cacggttgcg tgagcgcgat aaagcgtttc acgcaaagag cggtcctgcg cgtattgcat
     1201 caccggcaga tagcagggca ttttaagggt aagtttccag ccgtccagcc ccgcttcttg
     1261 cgcggcggct tgcgcagcct gtctgacatc acggggcaga ccggccaatc gattttcgtc
     1321 ctgaatatgc agcgaccagt tgtcgataga gtcgagcatg ttctcggaaa atttttgcga
     1381 tgcctgagct tcctgatcgg aaatttctgc gtaccgttcg cgttgtttgc cttgcaattc
     1441 gacgccgctc aggcgaaagt cgcgcaaggc caagtccacg attcgctgcc gtgtgggcga
     1501 cagcccggcg aatgcgggat cggccttcag acgcttgtat tgcgcgtaca ggccggtatg
     1561 caatccggtc cacgttgaaa attccgtaat gagcggcagg cactggttat aggcttcgcg
     1621 tagcgccggc gtattcacga cggcgttcag gtgtccggcc accgaccagg cccgccacaa
     1681 cctttcagag gcgtcctcaa gaggctcgat gaaagacgcc caacttgttc cggtgtgcgg
     1741 gtcggcggcc ttttctacag cggcccgggc gtcgcggatc agtccactga cggcgggctc
     1801 gatatgctcg ggttcgatcg ccgcgtaatc aataagggaa tcaataggcg cgagcagggg
     1861 attggaattc attagggttg tcacagctta taagtcttga tgcgcggcat tgcgctcggc
     1921 cgcttccagt gtattcacca gcaacatggc tatcgtcatc gggcccacgc cgccaggcac
     1981 aggcgtgatg gcgcctgcca cctttttggc cgattcaaat tccacgtcgc ccatcagctt
     2041 gccatcggcg ttgcggttga ttcccacatc gatcactatg gcgcccggtt tgatcatgtc
     2101 gcctttgaca atacctggct tgccggtcgc cacgaccagt atatcggcgc gacgcgtctg
     2161 ggcagccaga tcgcgggttt tcgaattgca aagagtaacg gttgcgccag cggccagcaa
     2221 cagcatggcc ataggcttgc cgacgatgtt gctggcgccg acgatgacgg cttcagcccc
     2281 ccacacggtc gcgccttcgg cttgcagcat tttcatgatt ccataaggcg tgcaggggcg
     2341 gattgacggc cgtcccgtca tgagcaagcc ggcattactg acatggaagc cgtcaacgtc
     2401 cttttcgacg gcgatcgttt caataacctt tcccggacgt atgtgcggcg gcaaaggcag
     2461 ttgcaccaaa atgccatgaa cgcttgcgtc gtcgttaagt tttttgacga catccagcaa
     2521 ctgttcctcg gacacgctgc tttccagccg tatcactttg gaagccagcc ccgctttttc
     2581 gcaggcggcg gctttgttgc gcacgtaaac agccgaggcg gggttgtccc cgaccagcac
     2641 caccgcaaga ccgggcgtga tgcctttctt tttcagatcc aaaacgcgtt tggcaacgtc
     2701 ggccttaata gcagcggata acgctttccc gtcaatgatg cgcgccgtca tgccgcgcct
     2761 tgcgccttgt tcagggcgat tttcatcaga tccgccaccg tcgtgacctc gagtttttcc
     2821 atgatgttgg ctcgatgagc ttcgaccgtt ttaatgctga tgttgagatc acccgcgatt
     2881 tgcttgttca ggcgacccgc cacgattcgt tccagcactt gctgttcgcg tgtggttaaa
     2941 cggctaagga cagcctggtg gctcttcaaa acttggttct gtgttacgcg ctcgcacgct
     3001 tgctcaagca tgcgcgcaac gatggtgcgc aaatcggttt cgttgaaagg cttttcaagg
     3061 aagtcgacag cgcctttttt catggtggac actgccatcg gcacgtcgcc gtggccggta
     3121 atgaatacga tgggtagcgg agccttgcgc gcgatgagtt cttcctgcag ttccaggccg
     3181 ctcatgccgg gcatgcgcac atcagcaatc agcactccca cctgatccgg atcgtatgcg
     3241 tttagaaatt cttcagctcc gccgaaactt ttaacgcgat agccgttggc ctcaaggagc
     3301 cagcgcagcg aatcgcgcac ggcttcatcg tcgtccacga tgaaaatggt gctcgatgat
     3361 gtttgatgtg tagtcatgca tgcaactcct ggggggattc ttccgaggcg ggctcttcag
     3421 gcgtcgcaca gggcagggta aagcgaaaga tagtgccgcc atcgggattg gtgctagccc
     3481 aaagacggcc atgatgcgat tcaataatag tgcggcagat atttagtccc atgcccaggc
     3541 cttccgactt ggtgctatag aagggttcaa acaatcgttc ggggtcgcgc agaccgtaac
     3601 cgcgatcgat aacggctatt tcaacaagcg ggctttgccc ggtcacgacc acatgcagaa
     3661 cgcggtactc gcttttttcc atggcttcaa tgccattttt cagcagattt aataatacct
     3721 gttcaatcag aatggggtcc gccacgacgt ccggcaatga atcgggcagg tcctgttcaa
     3781 tggtcacttg gcgcttgcga gcgtctattt cggcaaaccc gatcgcatta tccagaatgc
     3841 gcttgatcac gacgcgctgg cggcgcggct cgctgcgttt gacgaattcg cga
//
LOCUS       DATCXU010000179         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_401643,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000179 DATCXU010000000
VERSION     DATCXU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401643"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIR04_03980"
     CDS             complement(<1..396)
                     /locus_tag="VIR04_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY9459938.1"
                     /translation="MRPVKVGLLGLGVVGRGTWKVLNDNAQEIARRAGRRIEVVAVAA
                     RDVAKARRLVGDHVLVTTDGMELVSHPDIDIVVELIGGQTVARKWVMAAIEQGKHVVT
                     ANKALLAMHGNEIFAAARRRSVMVAFEAAV"
     gene            complement(393..1580)
                     /gene="alaC"
                     /locus_tag="VIR04_03985"
     CDS             complement(393..1580)
                     /gene="alaC"
                     /locus_tag="VIR04_03985"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEY9459939.1"
                     /translation="MTSFPRIERLPPYVFNITSELKMAARRRGEDIIDMSMGNPDGPT
                     PRHIVDKLIEVASRPDTHGYSVSKGIPRLRRAISGWYERHYGVQIDPDTEAIVSIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAVIAGANIRSVPMTPGLDFFEEIERAVR
                     ESIPKPKMMILGFPSNPTAQCVDLSFFERIVALAKEHNILVVHDLAYADITFDGYVAP
                     SIMQVEGARDVAVEFFTMSKSYNMAGWRVGFMVGNRELVNALARIKSYHDYGTFTPIQ
                     VAAIAALEGPQECVGRVVEQYQNRRDVLAKGLHEAGWMVDIPKATMYIWAKIPEFYQA
                     AGSLEFSKRLLAEARIAVSPGIGFGEYGENYVRFALIENEQRTRQAVRGIKEMFRKDG
                     LNK"
     gene            1770..2222
                     /locus_tag="VIR04_03990"
     CDS             1770..2222
                     /locus_tag="VIR04_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY9459940.1"
                     /translation="MELHVETNPAFNTVTAYGHDYIEINAVPYSTAIYFSPEGEIKTW
                     AVETAADINADLLREVAGLTQAVSDPMAFLDAQPASPKPIDAPEVILIGTGPRQHFLA
                     SGVTRELQDMGIGIETMDTRAAARTYNILMAEGRRVIAALLPPKDKAQ"
     gene            2222..2686
                     /locus_tag="VIR04_03995"
     CDS             2222..2686
                     /locus_tag="VIR04_03995"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY9459941.1"
                     /translation="MQATVGKPLPDFVAIGTQGEVSPASLAGRRAVLYFYPKDNTPGC
                     TTEAQNFRDFYNDFLAANCQVIGISRDSLKSHDGFIAKQNLPFPLVSDSDESLCAVFN
                     VIKMKNMYGKQVRGIERSTFLIDARGILVQEWRRVRVAGHVDEVLQAARVVA"
     gene            2755..4383
                     /locus_tag="VIR04_04000"
     CDS             2755..4383
                     /locus_tag="VIR04_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY9459942.1"
                     /translation="MSLPPLPTRMGAAITAPATRFDATSDSQKEVALLTETKQIVARQ
                     NNIVPAISPVAEPPLLSIVAPKTRKRTRLAVARRKFFVLDTNVLLHDSNSLFKFEEHD
                     IFLPMIVLEELDHQKKGMSEVARNARQVSRFLDGLVSGSDQLAKGTLLDSLGNQEALG
                     NLHFQTTAMNAKLPIELPLGKADNLILNVVHALQSLHPKRQVILVSKDINMRLKARAL
                     GLHAEDYLNDHVLDDSDLLYDGVLPLPENFWAKHGKDVESWQQSGTTYYRIKGPLCAE
                     FIVNEFVYFEGELPLYAQVKEVNGKSAVLATLRDYSHGKNNVWGITARNREQNFALNL
                     LMNPDCDFVSLLGQAGTGKTLLALASALTQTLETRRYTEIIITRATVPVGDDIGFLPG
                     TEEEKMLPWMGALEDNLEVLHLGAGASDTPSTAGGPDWSKNPTMELIRSRIKVKSLNF
                     MRGRTFLNKFLIIDEAQNLTPKQMKTLVTRAGPGTKIVCLGNIAQIDTPYLTEGSSGL
                     TYVVDRFKGWPHAGHITLQRGERSRLADYASEAL"
     gene            4402..>4553
                     /locus_tag="VIR04_04005"
     CDS             4402..>4553
                     /locus_tag="VIR04_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9459943.1"
                     /translation="MPGSIPVGITMGDAAGIGPEIIVKLFAAGLPQATVVYGDQGILN
                     ATIKRLG"
BASE COUNT         1064 a         1340 c         1195 g          954 t
ORIGIN      
        1 cacagcggcc tcgaacgcga ccattacact ccggcggcgc gcggcggcaa atatttcgtt
       61 gccgtgcatg gcgagcaacg ccttattcgc cgtgacgaca tgcttgccct gctcgatcgc
      121 cgccattacc catttgcgcg cgactgtctg cccgccgatg agttcgacca caatatcgat
      181 atcgggatgg cttaccagct ccattccgtc ggtggtgacc agaacatgat cgcccaccag
      241 gcgccttgct ttcgcaacgt cgcgcgccgc cacggcgacc acttcgatac gccttcccgc
      301 ccgtcgggcg atctcttgcg cattgtcgtt caacaccttc catgtgccgc gcccgaccac
      361 gcccaagccc agcaaaccaa ctttgacagg cctcatttgt tcagcccgtc cttgcgaaac
      421 atttctttaa tgccacgcac tgcctgacgg gtgcgctgtt cgttttcgat aagcgcgaaa
      481 cgcacgtagt tctcgccata ttcgccaaag ccaatgccag gcgaaacggc aattcgcgct
      541 tcggccagca atctcttgct gaattcaagt gatcccgctg cctgataaaa ctcggggatc
      601 ttggcccaga tatacatcgt cgccttggga atatcgacca tccagccggc ctcgtgcaaa
      661 cctttggcca ggacgtcacg gcgattctgg tactgttcga ccacccggcc cacgcactct
      721 tgcgggcctt caagggctgc aatcgcagcc acctggatag gcgtgaatgt gccgtaatca
      781 tggtaacttt tgatgcgcgc caacgcattg acgagttccc ggtttcccac catgaaaccc
      841 acgcgccagc ccgccatgtt gtaacttttg ctcatcgtga aaaactcgac ggccacatcg
      901 cgggcccctt cgacctgcat gatggatgga gcaacatacc cgtcgaatgt aatatccgcg
      961 taggccagat catgcacgac caggatatta tgttccttgg caagcgccac aatacgttca
     1021 aaaaacgaaa gatccacgca ctgcgccgtc ggattgctgg gaaaacccag aatcatcatt
     1081 tttggcttcg ggatgctttc gcgcacggcg cgctcgattt cctcaaaaaa atcaagaccc
     1141 ggcgtcatgg gcaccgaacg gatattggcc ccggctataa cggccccata aatatgaatc
     1201 ggatagctgg gattaggcac aagcactgta tcgccgcgat ccagcgtcgc cagcatcagg
     1261 tgggccaggc cttctttgga gccgatagac acaatcgcct cggtgtccgg atcgatctga
     1321 acgccgtaat gacgctcata ccatcccgat attgccctgc gcaatcgcgg aatccccttc
     1381 gacacggaat agccgtgcgt gtcgggccgg ctggccacct caatcaattt atcgacaata
     1441 tgcctgggtg tgggtccatc aggatttccc atggacatgt caatgatgtc ctcgcctcgc
     1501 cgccgcgccg ccatttttaa ctcgctggta atgttaaaaa cgtaaggcgg taagcgttct
     1561 atgcgaggaa aactcgtcat gatcaatcct tggggatgat aataatgaaa aatggccggt
     1621 ttcgttgcag cgcggcgatg ccgccaaggc aggcggcgca ttgcgcgcaa caaatagctg
     1681 actagtttaa tcgatcccaa aaaactataa aaaccgcgat ttgaaagacg ggtttgcgct
     1741 atgatcgtta tgacttcagg aggttttttg tggaattaca tgtggagaca aatccggcct
     1801 tcaacaccgt gacggcctac ggccacgatt acatcgaaat caatgccgtg ccatacagca
     1861 cggccatcta cttcagtccc gaaggcgaaa taaaaacatg ggcggtcgaa acggcggccg
     1921 acatcaacgc ggatctgctg cgcgaagttg ccggactgac ccaggccgtc agcgacccga
     1981 tggcctttct ggatgctcag cctgcgtcgc ccaaacccat cgatgcgccc gaagtcatac
     2041 ttatcggcac aggtccgcgg caacattttt tagccagcgg cgtaactcgc gaattgcaag
     2101 atatggggat cggcattgaa acgatggaca cccgcgccgc cgcgcgcacc tacaatattt
     2161 taatggccga gggacgccgg gtaatcgcgg cattactgcc gccgaaggac aaagcgcaat
     2221 gatgcaggcc actgttggaa agcccctgcc cgatttcgtc gccataggca cgcaaggcga
     2281 agtcagcccc gcttcgctcg cgggccggcg cgccgtcctg tatttttacc cgaaggacaa
     2341 tacgccgggc tgcacgaccg aagcgcaaaa ttttcgggac ttctacaacg actttcttgc
     2401 agcaaactgt caggtaatcg gaatttcacg cgattccctg aagtctcatg atggctttat
     2461 cgccaaacaa aatctgcctt tcccgctagt ctcggatagc gacgaaagcc tgtgcgcggt
     2521 atttaacgtc atcaaaatga aaaatatgta cggcaaacaa gtgcgcggaa tcgaacgcag
     2581 cacttttttg attgacgccc gtggaattct agtccaggaa tggcgccgcg tacgggttgc
     2641 cgggcatgtc gacgaagtgc tgcaagcggc gcgagtcgtt gcctgaccag aacgccggcc
     2701 cggggccatt tcgatcgcca ctcatacgtc atgaccatca ggagttctca ttttatgtcg
     2761 ctaccaccac tgcctacccg catgggagcc gccatcaccg cgccggcaac ccgtttcgac
     2821 gccacttctg attcacaaaa agaagtcgcc ttgctgaccg aaacgaaaca gatcgttgcg
     2881 cgccagaata atatcgttcc ggctatttca cccgtggccg aaccgccgct gctgtccatt
     2941 gttgcgccaa aaaccaggaa aaggacacgc ctggccgtag cccgccgcaa attcttcgtg
     3001 ctcgacacca acgtgctgct gcacgactcg aattcgctgt ttaaattcga agaacacgat
     3061 atttttctgc cgatgatcgt gctcgaggag ctggatcacc aaaagaaagg catgtcggaa
     3121 gtggcccgca acgccagaca agttagtcgc tttcttgacg gattggtcag cggttctgac
     3181 caattggcga aaggaacgct gctggactcg ctgggcaatc aggaggcact gggcaatttg
     3241 cattttcaaa cgacggccat gaatgcgaaa ttgcctatcg agctgccttt gggcaaagcc
     3301 gacaacctga ttctgaacgt cgtgcatgcg ctgcagtcgc tgcacccgaa gcggcaggtc
     3361 atattggtgt ccaaagatat caacatgcgg cttaaggccc gcgctttggg cctgcatgcc
     3421 gaagactatc ttaacgacca tgtcctggat gattccgacc tgctgtatga cggggtcttg
     3481 ccgctgcctg aaaacttctg ggcaaaacat ggcaaagacg ttgaatcctg gcaacaaagc
     3541 ggcacgacct actaccgcat caaagggcct ttgtgcgccg aatttatcgt taatgaattc
     3601 gtctacttcg aaggcgagct gcctctttat gcccaagtga aggaagtcaa tggcaaaagc
     3661 gcggtgctgg ccaccttgcg cgattacagc catggcaaga ataatgtgtg gggcattacc
     3721 gcgcgaaatc gcgagcaaaa tttcgctctc aacctgctca tgaatccgga ttgcgatttc
     3781 gtgtcgctgc tgggccaggc ggggaccgga aaaacattgc tggctttagc cagcgccctg
     3841 actcaaacct tggaaacacg gcgctatacc gaaatcatca ttacacgggc gaccgtcccg
     3901 gtaggcgatg atatcgggtt tctgcccggc accgaagaag aaaaaatgct gccatggatg
     3961 ggcgcgctcg aggacaacct ggaagtgctt catctgggtg cgggcgcgag cgacacacca
     4021 tcaaccgccg gcggcccgga ctggtccaag aacccgacca tggaacttat tcgctcgcgc
     4081 atcaaggtga aatcactgaa tttcatgcgc ggccgaacgt tccttaataa atttctgatc
     4141 atcgacgagg cgcagaatct gacgcccaaa caaatgaaaa cattggtgac ccgagccggc
     4201 cccggcacaa aaattgtctg ccttggaaat atcgcgcaaa tcgatactcc ctacctgacc
     4261 gagggaagct cgggcctgac ctatgtggtg gaccgtttca aggggtggcc gcacgcgggg
     4321 cacattacgt tgcaacgtgg ggaacgctct cgcctggctg attacgccag cgaggccctg
     4381 taaccgatgg gccgccccac cttgcccggt tcaatccccg tgggcatcac aatgggcgat
     4441 gcggcgggca ttggtcccga aattatcgtc aaactattcg ccgccggcct gccccaggcc
     4501 acagtggtct atggcgacca gggcattctg aacgcgacga tcaaacggtt ggg
//
LOCUS       DATCXU010000180         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_242317,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000180 DATCXU010000000
VERSION     DATCXU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242317"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VIR04_04010"
     CDS             complement(<1..635)
                     /locus_tag="VIR04_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY9459944.1"
                     /translation="MPLHRLAALFRPSSVLVLSERALPIGRDTPDLLKNGITFTVITA
                     QSIRVPAELDGVASGARLDLALLCVDPRRLPEALEAVRAHRPRAIALLAASAPSADPI
                     ADVAVCQSWARLNDCLILGPRSFGVQRPPRGLNFSHAPNMALPGRVALVTQSRSIVAA
                     VMDWAADINMGFSAIISLGDEAVVTIPEVLNYLAADGHTDSIALYLDTISSS"
     gene            745..2154
                     /gene="gltX"
                     /locus_tag="VIR04_04015"
     CDS             745..2154
                     /gene="gltX"
                     /locus_tag="VIR04_04015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY9459945.1"
                     /translation="MTSPISSVVRTRFAPSPTGFLHLGGARTALFSWAFARHNKGTFV
                     LRIEDTDLERSTPEAVKAILDSMAWLGIQPDEGPFYQMQRMDRYREVIGQMLADGTAY
                     HCYSTPQEIEAMRALARASGRKPRYDGTWRPEPGKTLPSIPPGRKPVVRFKNPQEGAT
                     GWDDMIKGPISFDNAELDDLIIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTP
                     RQINILRALGAALPQYGHVPMILGPDGEKLSKRHGSVNVMEYDKQGYLPEAMINYLAR
                     LGWSHGDEELFSREQLIDWFDTRNLSKSAAQWDPKKLNWVNAHYIKQSSDEDLAIRVA
                     PRIRELGGDPEAVQLTPVVALLKDRAETLNQLADGAMLFCAAFTAPSSELQTQYLDEE
                     TKQVLADFAQRARALPDWTTPAIEALIHSLLTDRNIKMKKLGIPLRVAVTGRKQTPAI
                     GAVLTILGRDTVLARLAAL"
     gene            complement(2168..>2586)
                     /locus_tag="VIR04_04020"
     CDS             complement(2168..>2586)
                     /locus_tag="VIR04_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9459946.1"
                     /translation="PAHSTAVAPVHKARLCQAPRANDALIGQWLSIHKQRGIVGQLQI
                     LFTLSPDGTMAYAEQLRRGKKPSQGLHETGCWRHEGQTLVLQTTKSNGVPVELSDPIY
                     TNRYRILAQTGKALSLQRAHGPISARRMPDGYRLPF"
BASE COUNT          599 a          779 c          715 g          493 t
ORIGIN      
        1 gaggaagaaa tggtgtccag atatagcgcg atactatcgg tatgcccatc agccgctaaa
       61 taatttaata cctcgggaat cgtaacgact gcctcgtcgc cgagcgagat gatcgccgaa
      121 aaacccatat tgatatcggc agcccaatcc atgaccgctg caacgatcga acgcgactgg
      181 gtaaccagcg ccacacgacc tggcaatgcc atgtttggcg cgtggctgaa attcagtcca
      241 cgcgggggcc gctgcacgcc gaatgatcgc ggcccaagaa tcaggcaatc gttcagccgt
      301 gcccacgact gacaaactgc aacatctgca atcggatcgg ccgatggcgc cgacgccgcg
      361 agcagcgcaa tcgcccgcgg gcgatgcgcc cgaaccgctt ccaacgcttc tggcaagcgc
      421 cttggatcaa cacacagcaa cgccaaatca aggcgggcgc cgcttgcaac gccgtcaagt
      481 tcggcaggaa cccgaattga ttgtgccgta atgacagtga atgtgatgcc gtttttcagc
      541 aaatcgggtg tgtcgcgccc gatcggcaac gcgcgctcgc ttagcaccag caccgagctg
      601 ggcctgaaca aagcggcaag gcgatgaagc ggcatttgtt tatccgtttt caacaataat
      661 taatctgtac tttaaaatgc gttgttggct aacgccaatt gtgcccgaga tcgactcgcg
      721 catgacacaa tccgaacact aactatgaca tcacctattt cttccgtggt tcgcacacga
      781 tttgccccct cgcccacagg ctttctgcat ctgggcggcg ctcgcacggc cttgttttcg
      841 tgggccttcg cccgtcacaa caaaggcact tttgtgctgc gcatcgaaga caccgatctt
      901 gagcgctcaa ccccggaagc cgtaaaggct attttagaca gcatggcctg gttgggaatt
      961 caacccgacg aagggccgtt ttatcaaatg caacgcatgg atcgctaccg cgaagtcatc
     1021 ggccagatgc tggccgacgg tacggcctac cactgctaca gcaccccgca ggaaatcgag
     1081 gcgatgcgag ctctggcccg ggcgtccgga cgcaagccgc gatatgacgg tacgtggcgc
     1141 cccgagccgg gcaaaaccct gccgtccatt ccgccagggc gcaaaccggt cgtgcgtttt
     1201 aaaaacccgc aagaaggcgc caccggctgg gacgacatga tcaaggggcc catcagtttc
     1261 gacaatgcgg aactcgatga cctgatcatt gcgcgtcccg acggcacccc tacgtataat
     1321 ttctgcgtcg tcgtcgacga ttgggacatg aaaatcaccc atgtgattcg gggagacgat
     1381 cacgtcaaca atacgccgcg ccaaatcaac atcttgcgcg cgctgggcgc cgctctgccg
     1441 caatacggcc atgttccgat gatcctgggt cctgacggag aaaaactgtc aaaacgccat
     1501 ggctcggtta atgttatgga atacgacaaa caaggctatt tgcccgaagc catgatcaat
     1561 tatctggcgc gactgggctg gagccatggc gacgaagaac tctttagtcg cgagcaactg
     1621 attgattggt tcgacacgcg caatttaagc aaatccgcgg cccaatggga tcccaaaaag
     1681 ttgaactggg tcaatgcgca ctacatcaag cagagcagcg acgaagacct cgcgattcgt
     1741 gtggcgccgc gtatccgcga actcggaggc gatcccgaag ccgttcagtt gactcccgtt
     1801 gtagccctgc ttaaagatcg cgccgaaaca ttaaatcaac tggccgacgg cgcgatgttg
     1861 ttctgcgctg ccttcactgc ccccagcagc gaactccaga cacagtatct ggatgaagaa
     1921 acaaaacagg tgctcgccga ctttgcgcag cgcgcccggg cgctgcccga ctggacaaca
     1981 cctgccatcg aagcgctcat tcactcattg ctgactgacc gcaacataaa gatgaaaaaa
     2041 ctgggaattc ccctgcgcgt ggccgtaacc ggacgcaagc agactcccgc aatcggcgcc
     2101 gtcctgacta ttttgggccg ggacacagtg ctggcccggc tggccgccct ttgaagggcg
     2161 agccgcatca aaacggcaat cggtaaccat cgggcatgcg ccgcgcacta atcgggccat
     2221 gcgcacgctg taaagaaagc gccttgccgg tctgagcgag aatacgatag cgattggtgt
     2281 aaatcggatc ggaaagctcg accggcaccc cgttggactt ggttgtctgc aacaccagcg
     2341 tctgtccctc gtgacgccag cagcccgttt cgtgcagtcc ctgcgacggc ttcttgccgc
     2401 gccgcagttg ctcggcgtaa gccatggtgc catcgggcga gagcgtaaac aggatctgaa
     2461 gctggccgac gatgcctcgc tgtttgtgaa tgcttaacca ctgtccgatg agggcatcgt
     2521 tggcccgggg agcctggcac aaccgagcct tgtgtacggg cgccacagcc gtcgaatgcg
     2581 ctggcg
//
LOCUS       DATCXU010000181         5014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_739006,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000181 DATCXU010000000
VERSION     DATCXU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739006"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VIR04_04025"
     CDS             <1..550
                     /locus_tag="VIR04_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9459947.1"
                     /translation="AEELGAIDILFNGAGYVHDGSILNCDLDAWNFSCNLNVTSMYYT
                     IRAFLPGMLEKKNGSIINMSSAASSIKGVPNRFVYGTTKAAVIGLTKSVAADFVARGI
                     RCNAICPGTVETPSLKGRIAAQAVKYGKSEQEIRAAFVSRQPVGRVGKPQEIAALAVY
                     LASDESSFTTGTAQIIDGGWSN"
     gene            581..1438
                     /locus_tag="VIR04_04030"
     CDS             581..1438
                     /locus_tag="VIR04_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY9459948.1"
                     /translation="MKLLRYGPVGHEKPACLDAEGGVRDLSGKIADITAADLSPQGLA
                     RLQDIDVSSLPRVANPGRIGTPYSGLGKLICIGLNYSDHAAETGSEIPKEPILFAKWA
                     QPTGPNDDVVMPKGSVKTDWEVELGVVIGTQARYVSLDNAMKHVAGYCISNDVSEREY
                     QMERGGTWDKGKGCDTFAPIGPWLATPDEVPDPQNLALWLEVNGKRFQNGNTKTMIFN
                     VAFLVHYISQFTTLEPGDLISTGTPPGVGLGQKPPVYLKAGDVMRLGIEGLGEQQQKV
                     VAWNASLHA"
     gene            1457..2767
                     /locus_tag="VIR04_04035"
     CDS             1457..2767
                     /locus_tag="VIR04_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HEY9459949.1"
                     /translation="MTTTITNLTVRDIRFPTSRQLDGSDAMNPAPDYSATYVVLETDA
                     SDHLQGHGLTFTIGRGNEVCVAGVKSLAPLVVGRTLDDIVANMGAFWRHITTADSQLR
                     WLGPDKGVMHLATAAIVNAVWDLWAKSAGKPVWKLLADLSPEELVRCLDFRFVTDVLT
                     PDEALAILRRNAPTKADREREMLEKGFPAYTTSAGWLGYSEEKIRRLAREGIAQGWTH
                     FKQKVGGNIEDDMHRAAVLRDEIGWDRKLMMDANQVWDVDEAIVNMRRLAAFDPWWIE
                     EPTSPDDILGHAAIRRRIAPIKVATGEHCHNRVMFKQLLQAGAIDFCQIDSARLGGLN
                     EVLIVLLMAAKFGVPVCPHAGGVGLCEYTQNIALFDYIGVSASLEGRVLEYVDHLHEH
                     FLEPVVMRNGRYMPPRKPGYSIQMHDQSLAQFQFPTGSAWQNQQ"
     gene            complement(2787..3962)
                     /locus_tag="VIR04_04040"
     CDS             complement(2787..3962)
                     /locus_tag="VIR04_04040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY9459950.1"
                     /translation="MPTLSKMTCVEDFRQLAEKRVPRMFYDYADSGSWTEGTYRSNER
                     DFQKLKLRQRVAVNMENRNIRRTMLGVDVAMPVAISPTGLTGMQHADGEILGARAAEK
                     FGIPFTLSTMSICSIEDIAAHTHKPFWFQLYVMRDKDFIERLIDRAKANNVSALVLTL
                     DLQMLGQRHKDIKNGLSAPPRPTLANMINLVTKPRWCVGMLGTKRRTFGNIVGHAKGV
                     GDLSSLSSWTAEQFDPALSWKDIEWIKNRWGGKLILKGILDAEDARYAVDSGADALIV
                     SNHGGRQLDGAPSSISALPSIAQAVGKEIEVLMDGGVRSGQDVLKAIALGANGVFIGR
                     AFLYSLGAFGEAGVTRCLELIANELDITMAFCGRTDINKVDRSILLNANLFDDPSRI"
     gene            complement(4047..4976)
                     /locus_tag="VIR04_04045"
     CDS             complement(4047..4976)
                     /locus_tag="VIR04_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY9459951.1"
                     /translation="MRTTRPAFPYDYQRIEATVDEFVPQWRTAGFDAVVAIARGGLVP
                     ATMASTALSLPLFALSYARADRRASWFTVARPATSSKVLLVEDIAGRGTTLLDSVNFL
                     RAAGYGVAIFSLAYDAESRITPDYGVKIPDGMGAWFPWERESITAAFGATSNQPDRAQ
                     HAYASWAIDLDGILLPDLAEHLYAEALVDTLARRDALTPHEILPSMDLSNVTIITGRP
                     EQDQERTRAWLNRYGFNGPLVMRDETRYRPDQTAQQKADAILERCHTHFIESNPGQAL
                     QIAARAKVARILWWDGAKALIVHAGDVENIDMA"
BASE COUNT         1055 a         1371 c         1485 g         1103 t
ORIGIN      
        1 ggccgaagaa ctgggcgcca tagacatttt gtttaacggc gccgggtacg ttcacgatgg
       61 gtcgattctg aattgcgatc tcgacgcgtg gaatttttcc tgcaatttga atgtgacgtc
      121 aatgtattac acgatccggg catttttgcc cggcatgctc gaaaaaaaga acggttcgat
      181 cattaatatg tcatcggcgg cgtccagcat taaaggcgtt ccgaaccgtt ttgtatacgg
      241 caccaccaag gcggcggtca ttggtttgac caagtcggtt gccgccgatt tcgtggcccg
      301 gggtattcgc tgcaatgcga tttgtccggg cacggtcgaa acgccttccc ttaaaggccg
      361 tattgcggcg caagctgtca agtatgggaa gtcagaacaa gaaatacgtg ccgcgtttgt
      421 ttcccgtcag ccggtgggcc gcgtcggcaa gccgcaagaa attgcggctc tggcggtgta
      481 cctggcgtcg gacgaatcgt catttacgac gggaaccgca cagattattg atggtggctg
      541 gtcaaattag gcagccgttt aagtttgagg agagtgaaca gtgaaattgt tacgttatgg
      601 tcctgtcggg cacgaaaaac ctgcctgcct tgatgctgaa ggcggtgtgc gggatttgtc
      661 cggaaaaatt gcggatatca ctgcggctga tttaagccca caggggctgg cgcgtctgca
      721 ggatattgat gtgtcttcat tgccgcgagt ggccaatcca ggtcgaatcg gcacgccata
      781 tagcggtctg ggcaagttga tatgcatcgg cctgaactat agcgatcatg cggccgaaac
      841 cggctccgag attccgaagg agccaatttt gtttgcgaag tgggcgcaac ccaccggacc
      901 aaatgatgat gtcgtcatgc ccaaaggttc ggtcaagacc gattgggaag ttgaattggg
      961 ggtggtaatc ggcacgcagg cgcgttatgt cagtctggac aatgccatga agcatgtggc
     1021 gggttattgc atttccaacg atgtgtctga acgcgaatat cagatggagc gcggcggcac
     1081 ttgggacaag ggtaaagggt gcgacacgtt tgcgcccatc gggccgtggc tggcgacccc
     1141 agacgaagtc cccgatccgc aaaacctggc gctgtggctg gaagtcaatg gcaagcgttt
     1201 ccaaaatggc aataccaaaa ccatgatctt taatgtggcg tttctggtgc actacatcag
     1261 ccagttcacg acccttgaac cgggcgacct gatctccacc ggaacgccgc cgggagtcgg
     1321 cctggggcaa aagccgcctg tctatcttaa agccggcgac gtcatgcgcc tgggtattga
     1381 aggcttgggt gaacagcaac agaaagtagt cgcctggaat gccagcctgc acgcataagc
     1441 ctttttcgag acattgatga ccaccactat tacaaatttg actgtccgcg atatccggtt
     1501 tcccacctcg cggcaattgg acggctccga cgcgatgaac ccggcgcccg attactcggc
     1561 gacctatgtt gttcttgaaa ccgacgcatc ggatcatttg cagggccacg gacttacgtt
     1621 caccattggg cgcggcaacg aggtctgcgt ggcgggcgtg aaatcgcttg ccccgctggt
     1681 tgtcgggcgt actctcgacg acattgtcgc caatatgggc gcgttctggc gccacattac
     1741 gacggccgac agtcagttgc gctggctggg tccggacaag ggcgtaatgc atttggccac
     1801 ggcggcgatc gtcaacgcgg tctgggatct gtgggccaaa tcggcgggca agccggtgtg
     1861 gaaattactg gccgacttaa gtcctgaaga gctggtccgt tgcctggatt ttcgtttcgt
     1921 cacggatgta ctcacgcccg acgaggcatt ggctattttg cgccgcaacg cgcccaccaa
     1981 ggccgaccgc gaacgcgaaa tgctcgagaa gggcttcccc gcatatacaa catcggcggg
     2041 atggctgggg tattccgagg aaaaaatacg ccggctggcg cgcgagggga ttgcgcaagg
     2101 ctggactcat ttcaagcaaa aggtcggcgg caacatcgaa gacgacatgc accgcgccgc
     2161 cgttctgcgc gacgaaatcg gctgggatcg aaagctgatg atggatgcga accaggtgtg
     2221 ggatgtcgac gaggcgattg tcaacatgcg tcgcctggcg gcgtttgatc cgtggtggat
     2281 cgaagaaccg accagcccgg acgacattct gggtcatgcc gccatccgcc ggcgcattgc
     2341 ccccatcaag gtcgccactg gcgaacattg ccataaccgg gtcatgttca agcagttgct
     2401 gcaagcgggc gcgatcgatt tttgccagat agattcggct cgtttgggcg gcttgaacga
     2461 agttctgatt gtgttgctga tggcggccaa attcggcgtc cccgtatgcc cccacgccgg
     2521 cggcgtgggc ttatgcgaat acacgcaaaa tattgcgctg tttgactaca ttggcgtttc
     2581 agcatcgctg gaaggccgcg tgcttgaata tgttgatcat ttgcacgaac attttctgga
     2641 gccggtcgtc atgcgcaacg gacgctatat gccgccgcgc aagcccggct atagcatcca
     2701 gatgcacgat caatccctgg cgcagttcca atttcccacc ggatcagcgt ggcaaaatca
     2761 gcaataacgg ccccggcggc ctgggcctag attcggctgg ggtcgtcgaa cagattggcg
     2821 tttaacaaga tggagcggtc taccttgtta atgtcggtac gcccgcaaaa cgccatggtg
     2881 atatccagtt cattcgcgat aagctccagg cagcgagtca caccggcctc accgaatgcg
     2941 cccagcgaat ataaaaaggc tcgtccgata aagaccccat tggcgcccag agcgatagct
     3001 ttgagcacgt cctgacccga acgcacgccg ccgtccatca ggacttcaat ctctttgcct
     3061 acggcctgcg ctatgctggg cagcgccgag atggaagagg gcgcgccgtc aagctgacgc
     3121 ccgccgtggt tggacacgat aagcgcgtcg gcgccgctat cgacggcgta acgcgcgtct
     3181 tcggcgtcaa gaattccttt cagtatcagc tttccgcccc accggttttt tatccattcg
     3241 atatccttcc agcttagcgc cgggtcgaat tgttcggcgg tccatgaaga cagtgatgac
     3301 aagtcgccca cgcccttggc atgaccgacg atattgccga aggtgcggcg cttggtgccg
     3361 agcatgccca cgcaccagcg tggtttggta accaaattga tcatgttggc cagggtgggg
     3421 cggggcggcg ccgacaagcc gtttttaata tctttatgtc gttgccccag catctgcaaa
     3481 tccagcgtca gcaccagcgc cgaaacatta ttggccttcg cccgatcgat caggcgttca
     3541 ataaagtctt tgtcgcgcat gacataaagc tgaaaccaga acggtttatg cgtatgggcg
     3601 gcaatgtctt caatcgagca aatgctcatg gtcgacaacg taaacgggat cccgaatttc
     3661 tcggccgcgc gcgcacccag tatctcgcca tccgcatgct gcattcccgt cagcccggtc
     3721 ggagaaatgg ccacgggcat ggcgacgtcc acccccagca ttgtgcggcg aatgttgcgg
     3781 ttttccatat tgacggccac ccgttgccgc agctttaatt tctggaaatc gcgctcgttg
     3841 gatcgatatg tgccttccgt ccatgaaccc gaatcggcat agtcatagaa cattctgggt
     3901 acgcgctttt cggccaattg gcgaaaatct tcgacgcagg tcattttaga taaggtaggc
     3961 aagaacggct cctgagtaat aatagtcaca attgtaccgg cgtgttgcac cgccggggtt
     4021 ctgtcgctgg cgacggtgtt gcccgtttac gccatatcaa tgttttcgac gtcgcccgcg
     4081 tggacaatca acgccttggc gccatcccac cacaggatgc gcgcgacttt ggcgcgagcg
     4141 gcaatttgaa gcgcttgacc cgggttgctt tcaatgaagt gggtatgaca gcgttccagg
     4201 atggcgtcgg ccttttgctg tgcggtctgg tcggggcgat agcgcgtttc gtcgcgcatg
     4261 accagtggac cattaaagcc gtatcggttt agccaggccc gcgttcgttc ttgatcttgc
     4321 tccgggcgcc cagtgataat cgttacattg gacagatcca tgcttggcaa tatctcgtgc
     4381 ggcgtcagcg catcgcgtcg agccagtgtg tcaacaagtg cctccgcgta caaatgttcg
     4441 gccagatccg gcagcaggat cccgtccaga tcgattgccc atgacgcgta agcgtgctgt
     4501 gcccggtcgg gctggttcga tgttgcgccg aacgcggcgg tgatggattc cctttcccag
     4561 ggaaaccatg cgcccatccc atcggggatc tttactccgt agtcgggagt aatgcgcgac
     4621 tcggcgtcgt aggcaaggct gaatatcgca acgccatagc cggcggcgcg caagaaatta
     4681 acactatcca atagcgttgt gccccggccg gcaatgtctt cgaccagcag cactttcgaa
     4741 ctcgttgcgg gccgagctac ggtaaaccag cttgcgcggc gatcggcgcg cgcataagac
     4801 aatgcgaaca agggcaggct tagtgcagtc gatgccattg tcgctgggac cagtcctcct
     4861 cgcgcaatgg cgacaaccgc gtcgaacccc gccgtgcgcc attgtggaac gaattcgtca
     4921 acggtcgcct caattcgctg gtagtcataa gggaaggcgg ggcgggtggt tcgcattgac
     4981 gggtctgttt agtgaaggcg aagcacggct ttct
//
LOCUS       DATCXU010000182         7236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_478442,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000182 DATCXU010000000
VERSION     DATCXU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478442"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VIR04_04050"
     CDS             <1..1126
                     /locus_tag="VIR04_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY9459952.1"
                     /translation="GWNTLDLKGNWHPKGSRHSVSFGTHFDQYTLVNPVYNTANWVSG
                     GNGSLYSDSRGKTGTSALWAQDAWELSPSVVATIGARYEWWRAFDGYNLSTTAGPDGR
                     SFGVRQPEVTQSGISPKLSVAWQANDQWSVTGSFGKALRFPTVGELYQNVQTGTTFTQ
                     ADPFLSPERVLSGELAIERNTQNGKLRLSLFEEHVSNALIGQTSTLPGVALPVSFVQN
                     IDATRQRGIELAAQQKDGLIKGLELSGSITYVDARITANGSYVPTTPGATSVGKRTPY
                     VPMWRAAVVATYRPDDRWAFTAAGRYSSRMYATVDNTDINPATYTGFESYFVTDLRVA
                     YKFDKHWNAAAGIDNLNNRKYFLYHPFPQRTIFAELKYNF"
     gene            complement(1129..2286)
                     /locus_tag="VIR04_04055"
     CDS             complement(1129..2286)
                     /locus_tag="VIR04_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY9459953.1"
                     /translation="MEAINQEAINQAAAISVPPIARIGDVMSAIDTPSLILDLDVFED
                     NLRKMQALAERHGVALRPHAKAHKCPEIALRQIALGAQGICCQKVSEAIPFIAAGVRD
                     IHISNEVVGAAKLDLLARLARQAAMTVCVDNARAVDALSAAMVGQGAAVGVLVDIDVG
                     QKRCGAQSSEEVVDLARLIQTRPNISFAGLQAYHGGLQHKRSLEQRKRSCAAVARLIR
                     SYVAALKKAGIDCPTITGGGTGTAVFDVASHVFTEIQAGTYAFMDTDYAGNDWGEGLA
                     FKHSLFLLGTVMSAPTADRAVVDIGLKSTSAESGAPQPLDDAGWSCVGISDEHSVLRA
                     KPGGSLPAWGAQVRLVPGHCDPTFNLHDSVVALRGQRVQALWPISARGLSR"
     gene            2443..2904
                     /locus_tag="VIR04_04060"
     CDS             2443..2904
                     /locus_tag="VIR04_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY9459954.1"
                     /translation="MFDILVYLFENYYTPQACPEADVLAHKLAAVGFEHEDIDDALGW
                     LHGLAQTTEQCVWLAQSPQSDSHRIFTEHEYHVLGAQAIGFIAFLENSRVLPPALREI
                     LIDRAQAANESPVSLDKIKIIALMVLWSQEAEVDHLVLEELLTDDHLRLSH"
     gene            2921..3775
                     /gene="gluQRS"
                     /locus_tag="VIR04_04065"
     CDS             2921..3775
                     /gene="gluQRS"
                     /locus_tag="VIR04_04065"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY9459955.1"
                     /translation="MANPGQRNYVGRFAPSPSGPLHLGSLVAAVASYADARAHQGQWL
                     LRIEDIDRPRVLAGADKFIMGQLQSLGMQWDGAPVWQSTRLDAYQAAFDALLTGRNVY
                     GCACTRQEINPSRPYAGTCRAGLPPGRTARSWRFRVPPGIERFTDRWQGPCEQDVEHQ
                     MGDFVVRRADGLWAYQLAVVVDDGAQGITAIVRGADLLESTAAQRVLARCLGIDYPSV
                     MHVPLARDASGRKLSKQNHAPALDCRDPVATLNLAWRQLGFDALDVGGLDQFWRMAPL
                     QWAARFEI"
     gene            3993..6701
                     /locus_tag="VIR04_04070"
     CDS             3993..6701
                     /locus_tag="VIR04_04070"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY9459956.1"
                     /translation="MNKTLIIAEKPSVAVDISRALGGFTREGDYFENDRFVLSSSVGH
                     LLTLVAPNDPVRGKWSFAHLPVIPNEFELGPADKRSGERLKLLVKLLKRKDVGAVINA
                     CDAGREGELIFRYIVQYAGVNKPIKRLWLRSMTQTAIREAFANLRDDETMKPLEAAAR
                     SRAEADWLVGINGTRAMTAFNSKDGGFFKTPVGRVQTPTLAIVAEREERIREFVPRDY
                     WEVHATFVAAAGFYSGRWFDPKFVKNELDAEQRDSRLWSQNAAQTIVAACREQPGHVT
                     EESKPSTQMSPALFDLTSLQREANSRFGFSAKTTLSLAQTLYERHKALTYPRTDSRYL
                     PEDYVPTVKQTMQALADPGSSAAARIRPFAAQLCKADWVKPIRRIFDNKKVSDHFAII
                     PTQQIPRELSEAEGKIYELIARRFLAVFFPAAEFRLTTRITKVSGHLFKTEGKVLTSP
                     GWLAIYGREAQGDDTNLVAVADGETVKTDEIEAKALVTKPPARYTEATLLSAMEGAGK
                     LIDDEALREAMSENGLGTPATRASIIEGLLNEGYLRREGRDLIPSAKTRQLMTLLSGL
                     GVNELTSPELTGEWEHKLKQIELRQLDRSAFMREIAQMTQVIVKHAKEYERDTVPGDY
                     ATLATPCPKCGGVGKENYRRYACTNCDFSIGKHPGGRTLEIPEVEELLSQRTLGPLPG
                     FISKMGRPFAALLRITDEYKLEFDFGQNDKDNAEPVDFSDQPALGACPKCGSDVYEHG
                     MNYVCEKSVGPERTCDFRSGKMILQQEITPAQITKLLTDGKTDLLSGFVSSRTNRKFK
                     AYLVKQPSGKIGFEFEARAEKPGAKSAAKKAVKTAAKTPAKTATKASGSKTAGKAPAK
                     KAAKAVAGKAVAGKAVKSAARKRAASKTAPRKAAAKASKNESDPPF"
     gene            complement(6731..>7236)
                     /locus_tag="VIR04_04075"
     CDS             complement(6731..>7236)
                     /locus_tag="VIR04_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY9459957.1"
                     /translation="SLPVLSGALHKTVVSTAVIMFVIANAGVFGFLLNRAGVPQALGE
                     WLSHVFYDKYTFLFGVNVALFVIGMFIETSASIVVLAPLLLPVAVHFGVAPTHFGVIM
                     VVNLALGMVTPPFGVNLFAACTVAKLPIERLVRPLFPFVGVVLACLMLITYVPAISLF
                     LKQLVYG"
BASE COUNT         1682 a         2231 c         2005 g         1318 t
ORIGIN      
        1 cggctggaac acgcttgatc taaaaggaaa ctggcatccc aaaggctctc gccacagcgt
       61 cagtttcgga acgcatttcg accaatatac gctggtgaat cctgtttata acaccgccaa
      121 ttgggtatcc ggcggcaatg gatcgcttta tagcgattct cgcggcaaga ccggaaccag
      181 cgcgctatgg gcgcaagatg cgtgggaact gtcgccatcg gtcgtggcga ccatcggcgc
      241 ccgctatgaa tggtggcgcg ccttcgacgg ttacaacctg tctacgaccg cgggacccga
      301 tggccggtcc ttcggcgtgc gccaacccga agtgactcag tcaggcattt cacccaagct
      361 ttccgtggcc tggcaggcga acgatcaatg gagcgtgacc ggatcatttg gcaaagcctt
      421 gcgattcccg acggtcggtg aactttacca aaatgtccag accggtacga ctttcaccca
      481 agccgacccg tttctgagcc cggagcgggt gctttccgga gagcttgcaa tagagcggaa
      541 cacacagaac ggcaaactgc ggttatcgct ttttgaagaa catgtttcaa acgcgctcat
      601 tggccagaca tccaccctgc ccggcgtagc cctgcccgta tcgttcgttc aaaatatcga
      661 cgcgacgcga cagcgcggca tcgaactggc cgcccaacaa aaagatggtt tgatcaaagg
      721 gctggaatta agcggcagca ttacctatgt cgacgccaga atcaccgcca acggaagtta
      781 cgtgccgacc acgcccggtg cgacatcggt cggcaagcgc acgccctatg tacccatgtg
      841 gcgagcggcg gtcgttgcaa cttaccggcc cgacgatcga tgggccttca ccgcggcggg
      901 ccgctatagc agccgaatgt acgccaccgt tgacaatacc gacattaacc cggcaacgta
      961 tacgggtttc gaaagctatt tcgtgaccga ccttcgcgtt gcgtacaagt ttgacaaaca
     1021 ctggaacgcc gccgccggca tcgacaacct caataatcgc aagtattttc tgtaccatcc
     1081 attcccgcaa cgaacgattt ttgcggaact gaaatacaac ttttaagatc agcggcttag
     1141 tccacgcgca ctaattggcc agagcgcctg gacgcgctgg ccgcgaagcg ccacgaccga
     1201 atcatgcaag ttaaaggtgg ggtcgcaatg accgggaacc aaacgcacct gagcgcccca
     1261 ggcgggcaag ctgccgcctg gcttggcgcg cagcacactg tgctcatcgc ttattcccac
     1321 acaactccat ccggcgtcat ccagaggctg tggcgcaccg ctttcggcac tggtggattt
     1381 cagaccaata tcgaccaccg cgcgatcggc agtcggcgcg ctcatgacag tgcccagcaa
     1441 aaacaggcta tgtttgaacg ccagcccctc gccccaatcg ttaccggcgt aatcggtgtc
     1501 cataaacgca taggtgcccg cttgtatttc ggtgaacaca tggctggcta catcgaagac
     1561 agcggtcccc gtgcccccgc ccgttatcgt cgggcagtcg atcccggcct tcttcagcgc
     1621 cgccacatac gaacgaatca aacgcgccac ggcggcgcac gaccttttgc gttgttcaag
     1681 agaccgttta tgctgcaggc cgccgtgata cgcctgcaag ccggcaaagc tgatattcgg
     1741 gcgcgtctgg atcaggcgcg ccagatccac cacttcttcg gacgactgcg ccccgcaacg
     1801 cttttgtccg acatcgatgt cgaccagaac cccgactgcc gcgccttgcc cgaccatggc
     1861 cgccgacagc gcgtcgaccg cgcgcgcgtt atccacgcac acggtcatcg ccgcctgtct
     1921 ggccagacgc gccagcaggt ccaatttggc ggcgcccacc acttcattgc tgatgtgaat
     1981 gtcgcgcacg ccggccgcaa taaacggaat ggcttcggat actttctgac agcaaatacc
     2041 ctgggcgccc aaggcaatct gccgcagggc gatctcggga catttatgcg ctttcgcatg
     2101 gggccgcaaa gcaacgccat gacgctcggc cagtgcctgc atctttcgca gattgtcctc
     2161 gaaaacatcc agatcaagaa tcaggctggg cgtatcgata gcgctcatga cgtcgccaat
     2221 ccgtgctatg ggcggcaccg aaatggcggc cgcctggttt atcgcttcct ggtttatcgc
     2281 ttccataatt cactcccccg gatttcgtgc caagcgtact cgatttaggg aaaagaatct
     2341 tccccggttg ctcgcaatgg taaatcagcg tacagttaat tccgacttta ccggttaacc
     2401 ctcaattatt cgttgactct gtaaagggca acccaaatcg ccatgttcga tatactggtt
     2461 tatttattcg aaaactacta cacgcctcaa gcgtgccccg aagcagatgt tctggcgcac
     2521 aagcttgccg cagtgggttt cgaacacgaa gacattgacg acgcgctcgg atggctgcat
     2581 ggcctggctc agaccacgga acaatgcgtc tggctggcgc aaagtccgca aagcgacagc
     2641 catcgcatct tcaccgagca cgaataccac gtgctgggtg ctcaggctat cgggtttatc
     2701 gcctttcttg aaaactcccg ggtactgcct ccggcgctgc gcgaaatcct tatcgacagg
     2761 gcgcaagcgg ccaacgaatc gccagtctcg ctggacaaaa taaagattat tgcgctaatg
     2821 gtgctctgga gccaggaagc cgaagtcgat catctggtgc tggaagaact gctgactgac
     2881 gaccacctgc ggctgtccca ttaaatcacc ggatgggccc gtggcaaatc ccggccaacg
     2941 caattatgtg ggccggtttg cacccagccc aagcgggcct ttgcatttag ggtcgcttgt
     3001 ggcggccgtg gccagctatg ccgacgctcg cgcgcaccag ggccagtggc tattgcgaat
     3061 cgaagatatc gatcgcccac gcgtccttgc cggcgccgac aagttcatca tgggccaatt
     3121 gcaatcgctg ggcatgcaat gggacggcgc gcccgtctgg caatcgacgc gactggacgc
     3181 ctaccaggcc gcattcgatg ccctcctgac cggccggaac gtttacggtt gcgcctgcac
     3241 gcgccaggaa ataaatccat cccggcctta cgctggcact tgccgcgccg gtctgccgcc
     3301 gggacgcacc gcacgatcgt ggcgtttcag ggtcccgccg ggcatagaac ggtttaccga
     3361 ccgctggcaa ggcccctgcg aacaagatgt ggaacaccaa atgggcgatt tcgtggtccg
     3421 ccgggccgac gggttgtggg cctatcagct tgccgtcgtg gtcgatgacg gcgctcaggg
     3481 catcaccgcc atcgtgcgtg gcgcggattt gctggaatca accgccgcgc agcgtgtgct
     3541 ggcgcgttgc ctgggcattg attatccaag cgtcatgcat gtgccgctgg cgcgagatgc
     3601 gtccgggcgt aaactatcca agcaaaacca tgctccagcg ctggattgcc gcgatcctgt
     3661 cgctacgctg aatctggcgt ggcgccaact cggctttgac gctctggacg tcggcggcct
     3721 tgaccagttc tggcgcatgg cgccgctgca gtgggccgcc cgctttgaaa tttaggcccc
     3781 tcgcgggggt caaatctgca gggaaccgct ccggcctata tactcgcaaa gtcgcgccgg
     3841 ccccttccag tgcaccgaac gagcattgac gggcccgctg caaccctttt tttaaggcaa
     3901 tagatacttt tggacacccc ggacttgcat ggagcgatta tcccctcgta tgatccgcgc
     3961 tatcgctgcc gaaacgcagc caatggaaac tcatgaataa aacgctcatt attgccgaaa
     4021 agccgtcggt tgcggtggat atatcccgtg ccctcggggg attcacacga gaaggcgatt
     4081 actttgaaaa tgaccggttc gtgctcagtt ccagcgtcgg acacttgctg accctggttg
     4141 cgcccaacga ccccgttcgc ggtaaatgga gcttcgccca tctgcccgtc atccccaacg
     4201 aatttgagct cggaccggcc gacaagcgat ccggcgagcg gctgaagctg ctcgtcaagt
     4261 tactgaagcg caaagacgtt ggcgctgtca tcaatgcctg cgacgccggg cgtgaaggcg
     4321 aacttatttt tcgctacatc gtgcaatacg cgggcgtcaa caagcccatc aaacgtttgt
     4381 ggctgcgctc aatgacgcaa acggcgatcc gggaagcttt tgccaacttg cgcgacgacg
     4441 aaaccatgaa gccccttgaa gcggcggccc gctcgcgcgc cgaggccgat tggctcgtgg
     4501 ggatcaacgg cacgcgcgcc atgacggcgt tcaacagcaa ggacggcggg ttcttcaaaa
     4561 caccggtcgg acgggtccaa acgcccacgc tggctatcgt cgccgagcgc gaagagcgca
     4621 tccgcgaatt tgtgccgcgc gactactggg aagtccatgc cacattcgtg gcggcggccg
     4681 ggttctacag cgggcgctgg ttcgacccta aattcgtcaa aaacgagctc gacgccgagc
     4741 agcgcgattc gcgcctgtgg tcgcaaaacg ccgctcagac cattgtcgcc gcttgccgcg
     4801 aacaaccggg acatgtcacc gaagagtcca aaccatcgac ccaaatgtcg ccggcgcttt
     4861 tcgatttgac ctcgctgcaa cgcgaagcca actcgcgttt tggcttttcc gccaaaacca
     4921 ccctgtcgct ggcgcaaacg ctgtatgagc gccacaaggc actaacctac ccgcgaacag
     4981 attcacgcta cctgcccgaa gactatgtac ccacggtcaa acaaaccatg caggctctgg
     5041 ccgaccccgg gtcctcggcg gctgcccgta tccggccgtt cgccgcgcaa ctttgcaagg
     5101 cggactgggt caagcctatc cggcgcattt tcgacaacaa gaaggtttcc gatcacttcg
     5161 ccatcattcc cacccagcag atcccgcgcg agctcagcga ggcggaagga aaaatttacg
     5221 agttgattgc gcgccggttc ctggcggtgt ttttcccggc cgcggagttc cgcctgacaa
     5281 cgcggattac caaagtatcg ggtcacctct ttaaaaccga gggtaaagtc ctgacttctc
     5341 cgggctggct ggcgatttac ggccgcgagg cgcaaggcga cgacacaaat ctggtggcgg
     5401 ttgccgacgg cgaaaccgta aaaaccgacg aaatcgaagc caaagccttg gtcaccaagc
     5461 cgcccgcgcg atacaccgaa gccacgctgt tgtcggccat ggaaggcgca ggcaaactca
     5521 tcgacgacga agccttgcgc gaagccatgt cggaaaacgg cctgggcacg cctgcgacgc
     5581 gggcatccat catcgagggc ttgctgaacg aaggctattt gcggcgcgaa gggcgcgacc
     5641 tcataccaag cgccaaaacg cgccagctca tgaccctttt atcgggactg ggggtcaatg
     5701 aactgacttc gcccgagctg accggcgaat gggaacacaa gctcaagcaa atagaattac
     5761 gccagcttga ccgcagcgcc ttcatgcgcg aaatcgcgca aatgacgcaa gtgatcgtaa
     5821 agcacgccaa agaatacgaa cgcgataccg taccaggcga ttacgcgacg ctggccacac
     5881 cgtgtcccaa atgcggcggc gtaggcaaag aaaattaccg ccgctatgcc tgcacgaact
     5941 gtgatttctc gatcggcaag catccgggag ggcgcacact ggaaattccc gaggtcgagg
     6001 agctgctaag ccagcgcaca ttgggtccgc tacccggctt catcagtaaa atggggcgac
     6061 ctttcgctgc actgttgcgg atcacagatg aatacaagct ggaattcgac ttcggccaga
     6121 acgacaaaga taatgccgaa ccggttgact tctcggatca gccggcgctg ggggcctgcc
     6181 caaaatgcgg cagcgacgta tacgagcacg gcatgaacta tgtatgcgaa aaatcggtgg
     6241 gcccggagcg aacctgcgac tttcgctcgg gcaagatgat tctgcaacaa gaaatcacgc
     6301 cggcgcaaat cacaaaactg ttgaccgacg gtaaaaccga tttgctctcg ggcttcgtgt
     6361 caagccgcac caaccgcaag ttcaaggcat atctggtcaa acagcccagc ggcaagatcg
     6421 gctttgaatt tgaagcccgc gccgaaaagc ccggcgccaa aagcgcagct aaaaaggcgg
     6481 taaaaacggc cgcaaagaca ccggcaaaaa cagcaacaaa ggcaagcgga tccaaaacag
     6541 ccggcaaggc cccggcaaaa aaggccgcca aagcggtcgc cgggaaagcg gtcgccggga
     6601 aagcggtaaa aagcgcagcc aggaaacgtg cggcaagcaa gaccgccccc aggaaagccg
     6661 ccgcaaaagc aagcaaaaat gaatccgatc cgccgtttta aggcgcttcg gtcccggccg
     6721 gtttgcgcca tcagccatag acaagctgtt ttaaaaacaa ggaaatcgca ggcacatacg
     6781 taatcagcat caggcaggca agcaccacgc cgacaaaagg aaataaaggc cgcaccaggc
     6841 gttcaatagg cagtttcgcc accgtgcacg ccgcaaaaag attgacgcca aaaggcgggg
     6901 tcaccatgcc cagcgccagg ttcaccacca taatgacgcc gaaatgggta ggcgcgacgc
     6961 caaagtggac agcaaccggc agcaacagag gggccagcac cacaatcgac gccgacgtct
     7021 cgataaacat cccaataaca aacaaggcaa cgttaacgcc gaataaaaaa gtgtacttgt
     7081 cgtaaaacac atgggacagc cattccccca gagcctgcgg cacgcccgcc cggttcagca
     7141 ggaacccgaa tacgcccgcg ttggcaatca caaacataat gacagccgtg gaaacgaccg
     7201 ttttatgcaa ggcgcccgac agaacgggca ggctca
//
LOCUS       DATCXU010000183         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_241825,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000183 DATCXU010000000
VERSION     DATCXU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241825"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VIR04_04080"
     CDS             complement(<1..555)
                     /locus_tag="VIR04_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY9459958.1"
                     /translation="MSNWLLPENLADILPAEARRIEELRRDLLDLYRTYGFELVAPPL
                     VEYLDSLLSGTGGDLNLRTCKLVDQLSGRTMGVRADITPQVSRIDAHLLNRQGIARLC
                     YCGTVLHARPAGLLSDRELLQIGAELFGHAGVEADIEIIQWALESVTRAGISHARLDL
                     NHPGVVRAIIDSDSALAGSVEAIFD"
     gene            complement(682..1569)
                     /gene="hflC"
                     /locus_tag="VIR04_04085"
     CDS             complement(682..1569)
                     /gene="hflC"
                     /locus_tag="VIR04_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY9459959.1"
                     /translation="MQRLYTALVGLVIVLIILSSCVFVVQQRDSALVFNLGEVRETIT
                     EPGLYFKFPPPFENVVFLDKRLQTIETKQPERVQTSEKKNLLIDSFVKWRIADPRLFY
                     VTFGANDRAAEERLQAQIRDALNASVNIRTVKDVIANQRDDIMKEVLTTVEQRAKPLG
                     VQVVDVRLRRIEFVPEISESVYRRMEAERKQEANRLRATGAAESEKIRAQADRQLQEV
                     VATAYAKAQTIKGQGDAKAASIYNEAYGKDPKFYSLYKSLEGYRAAFSKRGDMLVLSP
                     KSEFFKFWNSSGEAKPATP"
     gene            complement(1585..>2625)
                     /gene="hflK"
                     /locus_tag="VIR04_04090"
     CDS             complement(1585..>2625)
                     /gene="hflK"
                     /locus_tag="VIR04_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY9459960.1"
                     /translation="LIVQEGQVAVITKFGKYTKTLPPGLQWRMPYPIEDHQIVDISSL
                     RTFEVGFRGNALNKILPEALMLTNDENIVDVQFVVQYRLLPDGAPNYLFETSHPNESV
                     RQAAETAMREVVGKKPMDHILYSGRAEVATEVQKRIQSLLDDYKAGIQISTVAIQNVQ
                     PPEQVQAAFDDAVKAGQDRERQINEGNAYANKVLPEAQGRAARMLQEAEGYRAKVVGN
                     AEGDTARFTSVQAEFAKAPEVTRERLYLSTMQDILTSSSKIMVDSQASNNMLYLPLDK
                     IMQQASSAGATASDSSDTSALATAAAAGSSPSTPASTPAPKSSVPARSGARPSSDSSP
                     MQDSLSRNRFLR"
BASE COUNT          575 a          742 c          722 g          586 t
ORIGIN      
        1 gtcgaaaatg gcttcaacgc ttcccgccag agcggagtcg gaatcgatga tcgcacgcac
       61 tacgccgggg tgattcaggt caagccgcgc atggctgatg ccggctcgtg tcacgctttc
      121 aagggcccac tgaataattt caatatcggc ctcgaccccc gcatgaccga atagctcggc
      181 gcctatttgc aacaattcgc gatccgacaa tagccccgcc gggcgggcat gcaaaaccgt
      241 tccgcaataa cacaagcgcg caataccttg acgattgagc aaatgcgcat cgatgcgcga
      301 aacctgcggc gtgatatcgg cacgcacccc cattgtgcgg cctgacagtt ggtcgaccag
      361 cttgcaagtg cgtaaattta gatcgccgcc cgtgcccgac aataaagaat caaggtattc
      421 gaccagggga ggcgccacca gttcgaaacc gtaggttcga tacaggtcca gcaaatcacg
      481 gcgcaattct tcgatgcggc gcgcctcggc ggggagaata tcggccagat tttccggcaa
      541 taaccaattg gacattaaag agccctgaaa acaccattca aatacgacag tcaaataaaa
      601 agcgaccggg gggcgtaagc ccgccaagtc gcatgcggag acaatcttac ggtgtattgt
      661 accgtgttgc cgcgcagccg gctagggcgt agccggtttt gcctcgccgg acgaattcca
      721 gaacttgaag aattcagatt tcgggcttaa cacaagcatg tcgccccgct tggagaacgc
      781 ggcgcgatag ccttccaggc ttttgtaaag actgtagaac ttgggatcct tgccataagc
      841 ctcgttgtag atcgaagccg ccttggcgtc gccctggcct ttgatggtct gagcttttgc
      901 ataggcggtc gccacgacct cctggagttg gcgatcggcc tgcgcccgaa tcttttcgct
      961 ttctgccgca cctgtggcgc gtaaacggtt ggcttcctgt ttacgctcgg cttccatgcg
     1021 tcgataaacc gactcggaaa tctcgggcac gaattcaatg cgccgcaacc tcacgtccac
     1081 cacttgcacg cccagcggtt tagcccgctg ctcaaccgtg gtaaggacct ctttcatgat
     1141 gtcatcgcgt tgattggcga tgacgtcctt tactgtgcga atgttgaccg atgcgttcaa
     1201 tgcgtctcga atttgcgcct gaagacgttc ttcggctgcg cgatcgttcg cgccaaacgt
     1261 gacatagaac aaacgaggat ccgcaatccg ccacttgaca aaggagtcaa tcagcagatt
     1321 tttcttttcc gaagtctgaa cgcgttccgg ttgctttgtc tcgatggttt gcaagcgttt
     1381 atcaagaaag acaacgtttt caaacggagg tggaaatttg aaatacaagc ccggttcggt
     1441 gatggtctcg cgcacctcgc ccagattaaa caccagcgca gaatcgcgct gctgcacaac
     1501 gaagacgcat gatgacagaa taatcaaaac aatgacgaga ccgactaaag cagtataaag
     1561 acgttgcatt atgatggccc ccggttagcg taggaaacga ttgcgagaca gcgaatcttg
     1621 catcggtgaa gagtcgctgg acggccgggc gccagatcgg gcgggaacgc ttgacttcgg
     1681 cgcaggcgtc gatgccggcg tcgacggact tgagccagcc gccgccgcgg tcgccagcgc
     1741 gctggtgtcg gacgaatcgg acgccgtcgc gccagcgctg gatgcctgct gcatgatttt
     1801 atcgagcggc agatacaaca tgttattgct ggcttgcgaa tccaccataa ttttgctgct
     1861 gctcgtgaga atgtcttgca tcgtcgacaa atacaaacgc tcgcgcgtga cttccggcgc
     1921 cttggcaaat tcggcctgta cgctggtaaa gcgcgccgta tcgccctcgg cgttgccgac
     1981 gaccttggcg cgataccctt cggcttcttg caacatgcgt gcggcccggc cctgcgcctc
     2041 gggcaaaacc ttgttggcgt aagcattgcc ttcgttgatt tgacgttccc gatcctgacc
     2101 ggccttcacg gcgtcgtcga acgcggcttg cacctgttcg ggcggttgca cgttctggat
     2161 ggcgaccgta ctaatctgta tacctgcttt atagtcgtcc agaagcgact gaatgcgttt
     2221 ctgcacctcg gtggcgactt cggcgcggcc cgaatacagg atgtgatcca tgggcttttt
     2281 gccgactact tcgcgcatcg ccgtttcggc tgcctggcgc accgactcgt ttggatggct
     2341 ggtctcgaac agataattgg gagccccgtc cggcaacaac cgatactgca caacaaactg
     2401 gacgtcgacg atgttctcat cgttcgtcag catgagtgct tctggaagaa tcttgttgag
     2461 cgcattgccg cgaaagccaa cttcaaacgt gcgcaagctg gaaatatcga caatttgatg
     2521 atcttcgatg ggatacggca tgcgccactg caagcctggg ggcagcgtct tggtgtattt
     2581 gccaaacttg gtgattaccg ccacctggcc ttcctgaact ataag
//
LOCUS       DATCXU010000184         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_640210,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000184 DATCXU010000000
VERSION     DATCXU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640210"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..572)
                     /locus_tag="VIR04_04095"
     CDS             complement(93..572)
                     /locus_tag="VIR04_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEY9459961.1"
                     /translation="MFQKILLPIAVFIVILVALTFGETVINEAFAWLSYLSGLVLHNF
                     SDIYYAVQDYIARHTSRVVIALALTVPISFWIIKNKGQELTRRTSRRKIAIVLAIFLG
                     WLGGHRFYLGQIGWGITYLVIFYIFAPLVIVLSLIDAVRYIFMSDEEFTPSLKPTPR"
     gene            complement(634..1785)
                     /locus_tag="VIR04_04100"
     CDS             complement(634..1785)
                     /locus_tag="VIR04_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY9459962.1"
                     /translation="MSVLTHHATPGLATWMENDQVRSAMWRTECNSHLPKRIKVVDDT
                     LAADLAFRMANEGTAMLWRGDFQNAKQLLNALARRAKKEKQSKTNLAPHAAFNMYRLK
                     QSQRARLLNMLLIEVDANYQLNLRRAPDVSDACRVVFGESNEPFLLSLRALQGIIGSY
                     EWRKKGIAIPALEQSIHVHYGVFSPIRGEYIDLVNQAPLPTTELAFDIGTGSGVLAAV
                     LAWRGVKKIIATDVDPRALACARDNVERLGLNERIELMQADLFPPGKSALIVCNPPWL
                     PARPTSSIERAIYDPDNHMLLGFLNGLAEHLTPEGEGWMIISDLAEHLELRSREFLLQ
                     AIKQAGLRVVGKLDARPKHPKAFDDSDLLHEARAAEVTSLWRLVARDLG"
     gene            complement(1876..2769)
                     /locus_tag="VIR04_04105"
     CDS             complement(1876..2769)
                     /locus_tag="VIR04_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9459963.1"
                     /translation="MARPLNFTQIQAFKAVMQMGTATRAALMLNTTQPSISRRIAELQ
                     SATELKLFELHRGRLRPTSEGKLLYKTIQRHFDGLEKIESVVSIMRKSGTGVLRLGCT
                     PTLGIGLLPRAISKFLLKFPNTYINMQTLSTQQLADYLHQDLFDLVFTTGTLDQSDFQ
                     PSVIKNSPAVCVLPLQHPLKDNESINLKLLKGHRILSLNNDDEITIKIRCLLQANHIF
                     DEFPIETTSSITICALVAAGNGIAIVNPYVASIFSGQLLIKRFEPSIDVPIQMAMPSQ
                     TAPSALTHHFLDVLMDHVNAL"
BASE COUNT          672 a          689 c          808 g          705 t
ORIGIN      
        1 gtgcagcttc ggaaataagc gattcatcga atcgggcgct ctggcggtaa tcggagggaa
       61 aggttaaggt cttgaatcgc atgcgggcgg tcttagcggg gcgtaggctt taatgatggc
      121 gtgaattctt catcgctcat gaatatatag cgcacagcat cgatcaggct cagcacaatg
      181 accagtggcg cgaaaatata gaagatgacg agataggtga tgccccagcc gatctggccc
      241 aggtaaaagc ggtgaccgcc cagccagccc aggaaaatgg cgagcacgat ggcgattttc
      301 cggcggcttg tccgtcgcgt gagttcctgg cccttgtttt tgatgatcca gaaactaatg
      361 ggtactgtga gcgccagcgc gatgacgacg cggctggtgt gccgggcgat atagtcttga
      421 acagcatagt aaatatcact gaagttatgc aggacaaggc cgcttagata ggacagccat
      481 gcgaaggcct cgttgatcac tgtttcgccg aatgtcagcg ccaccaaaat gacgatgaaa
      541 acggcgatcg gcaataaaat tttctggaac atattccggc gtagcctctg gcattgaacg
      601 agaagtcgtg gtcagtttag cggctaatta ccttcagccc aaatctctgg caaccaggcg
      661 ccataacgac gtcacttcgg ccgcgcgcgc ctcatgcagc agatcgctgt cgtcaaacgc
      721 tttggggtgt ttgggccggg catccagttt tccaacaacg cgcagtcccg cttgtttaat
      781 tgcctgcagc aaaaattcac ggctgcgcaa ttccagatgt tccgccaaat cggaaataat
      841 catccaacct tccccttcgg gcgtcaggtg ctcggccagg ccgtttaaaa agcccagcaa
      901 catatggttg tcggggtcgt aaatggcccg ctcgatggaa gacgtggggc gtgccggcag
      961 ccagggcggg ttgcatacga tcagggcact ttttccagga gggaataaat ccgcctgcat
     1021 cagctcgatg cgttcattca atcccaggcg ctcgacgttg tcgcgggcgc aggccagcgc
     1081 cctcgggtcg acgtcggtcg cgattatttt ttttactccg cgccacgcca gaacggctgc
     1141 caatacgccg ctgccagtgc caatgtcgaa ggccagttca gtggttggca atggcgcctg
     1201 gttgaccagg tcgatatatt cgccccgaat gggggaaaat acgccgtaat gaacatgaat
     1261 gctttgttca agcgccggaa tggcaatgcc ctttttgcgc cactcatacg atccgataat
     1321 gccctgcaag gcgcgtagcg acaagaggaa tggttcgttt gattccccga aaacgacgcg
     1381 gcaggcatcg ctgacatcgg gcgcccgtcg caggttaagc tggtaattgg catcgacttc
     1441 aatcagcaac atgttcagca aacgcgctcg ttgcgattgc ttcagccggt acatgttgaa
     1501 ggcggcatga ggcgccagat tggtcttgct ttgtttttct tttttagccc ggcgcgccaa
     1561 ggcgtttaaa agctgtttgg cattttgaaa atcgccgcgc cacagcattg ccgtgccttc
     1621 attggccatg cgaaaagcca ggtcggcagc cagtgtgtca tcgacgactt taatgcgttt
     1681 gggtaagtgg ctgttgcatt cggtgcgcca catggcgctt cgaacttgat cattttccat
     1741 ccaggtcgcg aggcccggcg tggcatgatg ggttaataca gacatgaagg atgggtagtt
     1801 gctaaacaga ggcgattata gatgacggtt cgttgtcaag aagctcaact gttgcttgga
     1861 gggaacgctt ccagattaca gggcgttaac atggtccata aggacatcga gaaagtgatg
     1921 tgtgagcgcg gacggagcgg tttggctggg catcgccatt tgaatcggaa cgtcgattga
     1981 cggttcgaag cgcttgatca gcaattggcc ggaaaatatg ctggcgacat aggggttgac
     2041 gatggcgatg ccgttgccgg cggcaaccag cgcgcaaatc gttattgagg aagtggtttc
     2101 gattgggaat tcgtcgaata tatggttggc ctgcagcaaa catcttattt ttatagtgat
     2161 ttcgtcatca ttatttaagg acaatattcg atgacccttc aatagcttca ggtttatgga
     2221 ttcattgtcc tttaaagggt gctgcaacgg caatacgcag acggccggtg aattctttat
     2281 gacgcttggc tggaagtcgc tttgatccag cgtacccgtt gtaaagacta aatcgaacag
     2341 atcctgatgg agatagtccg ccagttgctg cgtgcttaga gtctgcatat tgatatatgt
     2401 attcgggaac ttcagcagaa atttactgat tgcacgaggc agcagcccta tgcccaaagt
     2461 tggggtgcaa cctaacctta aaactccggt gcccgatttg cgcatgattg agacgacgga
     2521 ttctattttc tccaacccgt caaaatgtct ttgaatcgtt ttataaagca gtttcccctc
     2581 gctggtaggg cgcaagcgcc cacggtgcaa ttcaaatagt ttcagttcag tggcgctttg
     2641 cagctctgct atacgacggc tgatggaagg ctgagtcgta tttagcatca gcgcggcgcg
     2701 tgtcgccgta cccatttgca ttactgcctt gaaagcctga atttgcgtga aattaagagg
     2761 tctggccata tcgtgcctta gcctgatgga ggagcatatg ctctgcgggg ctcgcctaat
     2821 gtatatcgaa tttgcataaa tctgcaataa gaaagaattg gactgtattg gata
//
LOCUS       DATCXU010000185         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_555732,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000185 DATCXU010000000
VERSION     DATCXU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555732"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VIR04_04110"
     CDS             complement(<1..190)
                     /locus_tag="VIR04_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY9459964.1"
                     /translation="MKDFNWANPYPTARIPVFARNVVSTSHPLAAQAGLRMMLKGGNA
                     VDAAVAAAATIVLVEPTSC"
     gene            complement(262..2655)
                     /gene="lon"
                     /locus_tag="VIR04_04115"
     CDS             complement(262..2655)
                     /gene="lon"
                     /locus_tag="VIR04_04115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY9459965.1"
                     /translation="MDNEKPDENAGPANEGKGARTVPDDALIIIPVRSMVLFPGLVVP
                     IVLGQERAVQAAQEAARAERQVGVVLQKEAEGAGQAAEGSLYQTGTVASILRYVTTPD
                     GLHHIICQGEQRFRVREFLDGFPFTVARVERLKEQQDISPQIEALIIQLKTRTAEVFQ
                     LLPQVPAELAAAAQSITSAGALADFVAGVMDLKPAEKQDILETLDLRSRLGKVLAFLV
                     HLVEVLRISHDIDQETKERFEDRQREVLLREQLKTIQKQLGETEGNAAEINDLREKIM
                     KAKMPAEVEAQANKELKRLESMSDASAEYSMLRTYLDWMIELPWSVVTEDRIDIAEAR
                     RILDQDHYGLRKIKQRILEYLAVRKLNPHGKSPILCFAGPPGVGKTSLGQSVAKAMGR
                     KFVRLSLGGVHDEAEIRGHRRTYIGALPGDIIQAVHKAGSKNCVMMLDEVDKLGMSAQ
                     GDPSSALLEVLDPEQNATFRDNYLAVPFDLSQVLFICTANMLETVPVPLRDRMEIISL
                     SGYTEEEKTQIALRYLVGRQLAANGLKPDQCEITDDAIRSIIHGYTREAGVRNLEREI
                     GSVFRHAAMGIAEGSVEHARIDKADLPAILGPEKFESELAMRTDLPGVATGLAWTPVG
                     GDILFIEATRAPGSGKLILTGQLGDVMKESAQAALTLVKARSGELGVAADWFEKGDIH
                     VHVPAGATPKDGPSAGVAMFVALASLATNVPVQSDHAMTGEISLRGLVLPIGGVKEKV
                     LAALQAGIKTVMLPARNKRDLDDIPEQARAQLKFVWLETVADALHEGLDTKAVQGAG"
     gene            complement(2681..3085)
                     /locus_tag="VIR04_04120"
     CDS             complement(2681..3085)
                     /locus_tag="VIR04_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY9459966.1"
                     /translation="MTVRKPAYSMWADAVNMLERADRLHRQFFRLEQGPTHGPTWEPP
                     ADVFETDRQLSVLVALPGVAPDQVKVGIEGGALLVVGHRPLPSLATAHIRRIEIPYGR
                     FERRIELPPGRFEICESTLANGCLLLSLLKLA"
     gene            3271..3948
                     /locus_tag="VIR04_04125"
     CDS             3271..3948
                     /locus_tag="VIR04_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY9459967.1"
                     /translation="MDLLARLSWKRVTTALVYRTPYKDTVRDGLLFFKAWRKNPTHIG
                     AVLPSGSALSAAITREIGLAQAPVLELGCGTGVFTRKMIERGVPAKELTLVELDPTFA
                     SRLRFSFPQAQILCIDASKLYKVQVSPGSKIGAAICGLPLLNMSAKKQLGILRGIFSA
                     MRPDGALYLFSYGLHCPVPLRLLDRMGLRSRKLDTVLLNVPPARIWKLTRRTNPGFNN
                     GQGSGQE"
BASE COUNT          867 a         1199 c         1048 g          888 t
ORIGIN      
        1 cgcaggaggt gggctccacc agcacaatcg tggccgccgc cgccacagcc gcatctaccg
       61 cgttgccgcc tttgagcatc atgcgcaagc cagcctgtgc cgccagcggg tgagaggttg
      121 acaccacatt tcgggcaaat acaggaatgc gagccgtcgg ataaggattg gcccaattaa
      181 agtctttcat acgcaccaac atgttatgga ataaagtgaa acttgcgaat caccgtcaac
      241 aaatacggcc caccgacgcc gctacccagc tccttgaact gctttggtgt ccaatccttc
      301 gtgtaacgcg tccgcaaccg tctccagcca tacaaacttc aattgcgcgc gcgcttgttc
      361 gggaatgtcg tccagatccc ttttattccg ggcgggcaac atcacggtct tgatgcccgc
      421 ctgcagcgcg gcaagcactt tttcttttac cccgccaatc ggcagcacca gcccgcgcaa
      481 gctgatctcg cctgtcatcg cgtggtcgct ttgaaccgga acattggtgg ccagcgacgc
      541 cagggcaaca aacattgcca cgccagcgct agggccatct ttgggcgtgg ctcctgccgg
      601 cacgtgcaca tgaatgtcac ccttttcgaa ccagtcggcc gcgaccccaa gttcgccgga
      661 ccgggctttg acaagggtta gagcggcctg agcgctttcc ttcatgacgt cgcccaattg
      721 tccggtaaga atcagtttgc cgctgcccgg agcgcgcgtt gcctcgatga acaggatatc
      781 gccgcctacc ggtgtccagg ccaggcccgt ggcaacaccc ggcaggtcgg tgcgcatggc
      841 caattcgctc tcgaactttt cggggcccaa tatggcgggc aagtcggcct tgtcgatacg
      901 tgcgtgttcg acagatcctt ccgcgattcc catggccgca tgacgaaaga cactgccgat
      961 ttcgcgttcg agattgcgca cacccgcctc gcgtgtatag ccgtgaatga ttgaacggat
     1021 ggcgtcatcg gtaatttcgc attgatcagg ttttagcccg ttggcggcca gttgccgtcc
     1081 aaccagatag cgcaacgcaa tttgggtttt ttcttcttcg gtgtaaccgg agagcgaaat
     1141 gatctccatc cggtcgcgca aaggaactgg aaccgtttcc agcatattgg cagtgcaaat
     1201 aaacagcact tgcgacagat cgaagggcac ggccagataa ttatcccgga acgtggcgtt
     1261 ttgctcgggg tcgagcactt caagcagcgc cgaagaagga tccccttgcg cgctcatgcc
     1321 cagtttgtcg acctcgtcga gcatcatgac gcagttcttg gaacctgcct tgtggacagc
     1381 ctgaatgata tcgccaggca atgccccgat atacgtgcgg cgatggccgc gaatttccgc
     1441 ctcgtcgtgc actccgccca gactcaggcg cacaaacttg cggcccattg ctttcgcaac
     1501 gctttgaccc agagacgtct tgccaacgcc gggaggcccg gcaaagcaca ggatcggact
     1561 tttgccatgc ggatttagct tgcgcacagc cagatattcg agaatgcgct gcttgatctt
     1621 gcgcaagccg taatggtcct gatccaggat tcgccgcgcc tcagcgatat ctatccggtc
     1681 ctcggtgacg accgaccagg gcaattcgat catccagtcc aaataagtgc gcaacattga
     1741 atattcggcc gaggcatccg acatgctttc cagccgtttc agttccttgt tggcttgcgc
     1801 ctcgacctcc gcaggcattt tcgccttcat tattttttca cgcagatcgt taatttccgc
     1861 tgcattaccc tcggtctcgc caagctgctt ttgaatggtc ttcaactgtt cccgcagcaa
     1921 cacttcgcgt tgacgatcct caaaccgctc tttcgtttcc tgatcgatgt cgtggctaat
     1981 acgcagcact tcgaccagat gcaccaaaaa cgccaaaact ttacccagtc gcgagcgcaa
     2041 atcgagggtt tcaagaatgt cttgtttttc ggcaggcttc agatccatga ctcccgccac
     2101 aaaatcggcc agagcgccgg ccgaagtaat gctttgcgca gccgccgcca gctcggcggg
     2161 cacttgcggc aataactgaa acacctcggc tgtgcgcgtc ttgagctgta tgatcaaggc
     2221 ttcgatttgc gggcttatat cctgctgttc tttaaggcgc tcgacccgcg cgacagtaaa
     2281 tggaaacccg tcaagaaact cgcgaacgcg aaaacgctgt tcgccctggc aaatgatatg
     2341 gtgcaaaccg tcgggggtgg tgacataacg caaaatactt gcaactgtgc cggtctgata
     2401 cagggaacct tcagcagcct gaccggcgcc ttccgcctct ttttgcaata caacgccaac
     2461 ctgtcgttcg gcgcgggccg cttcctgcgc cgcctggaca gccctttcct gccccaatac
     2521 gatagggaca accagtccgg gaaataacac catgctacga accggaataa tgatcaatgc
     2581 atcgtcgggc actgtgcggg cgcctttgcc ttcatttgcc gggcccgcgt tttcgtctgg
     2641 cttctcgtta tccatggcta ccttcccttg cgtcatgttt ttacgccaat ttaagcaaac
     2701 ttagcagcaa acacccgttg gccagcgtgg attcgcagat ctcaaacctt cccggcggca
     2761 attcgatacg gcgttcgaag cggccataag gaatctcgat ccgccggatg tgcgccgtgg
     2821 ccagcgaggg caagggacga tggccaacga ccagcaatgc gccaccttcg atgccgactt
     2881 taacttgatc gggagcaacg cccggcaatg cgaccagaac ggataattga cgatcagtct
     2941 cgaacacatc ggcgggaggt tcccacgtcg gaccgtgagt cggtccctgt tcgagccgaa
     3001 aaaactgtcg atgcaggcga tcggcgcgct caagcatatt cactgcatca gcccacatgg
     3061 agtaggccgg cttgcggact gtcataggtc agctcctgat cgtgcatcga tcgggatgca
     3121 cgccggtatg gagaaacggc gggagaaacg taattgagta ataatgcacc ttaattctgt
     3181 tacgttcaat aagccccgcc tggcgtaaac ttggcgagct atttattgga atcatttatg
     3241 tcgatatcaa aaataatact acgttcgccc gtggatcttc tcgcccggct ttcctggaag
     3301 cgagttacca ccgcgctcgt gtaccgcaca ccatacaaag acactgttcg ggatggacta
     3361 ttatttttca aggcatggcg aaaaaatccg acgcacatag gggccgtttt accatcgggt
     3421 tccgcgctat ccgccgcgat cacgcgcgag atcggtctgg cccaggcccc tgtcctcgaa
     3481 ctgggctgcg gaaccggggt atttacccga aagatgatcg aacgcggcgt cccggccaag
     3541 gaactgacac tggtcgagct tgatcccacg tttgcaagcc gtctgcggtt cagttttccg
     3601 caggcccaga tactgtgcat tgatgcatcc aagctttaca aggtgcaagt atctccgggg
     3661 agcaagatcg gggccgcaat ctgcggactt cctctactga atatgtcggc taaaaagcaa
     3721 cttggcatcc tgcgtggaat tttctccgca atgcgtccgg acggcgcgct ttatcttttt
     3781 tcttatggac tgcactgccc ggtcccactg cgcttgctcg acagaatggg cttgcggtcg
     3841 cgcaaactgg acaccgtgct gctaaacgtg ccgccggcaa gaatctggaa actgacacga
     3901 cgcacgaacc ccggcttcaa caacggccaa ggatcgggtc aggagtaaac aaatccgccc
     3961 attgcctggt cttttttaca accacccacc actgaaatcg gc
//
LOCUS       DATCXU010000186         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_215533,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000186 DATCXU010000000
VERSION     DATCXU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215533"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VIR04_04130"
     CDS             complement(<1..1388)
                     /locus_tag="VIR04_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY9459968.1"
                     /translation="MQSLRMKWQSLPRGAVVAITALAIYVPLSLIIIQSFLSAPFFMP
                     DKVFNLDAFHFIFADPDFPKALKSGLILAAGLAIIAVPLGSILAFLMSRTDLPGRHWI
                     EPLILVPVFVSPMVLGFGYVVATGPVGFFSLWAQELLGFVPWNVYSLTSIVIIAGLTH
                     VPYAYLYMASALHSMGSDVEEAARVAGAKPFQVMSSVSLPMVRPAMLYATVLLFFLGL
                     EVFGLMLVLGDPEGNLVMSTYLYKLTNKLGIPSYHLMAAVAMILIAITIPLVMLQRRL
                     MRSAERYVTLKGKASRARPLPLGKWRWPAGIIVALWLFVAVVVPLTGVLLRSFVSHWG
                     MGVSLFDVLSLQAFRTIWEQATLMRAIVNSVAIGVIGGALAVVCYTAIGLAMHRKPDG
                     VTRFLDYSVLVPRAVPGLLAGLAFLWVFLFVPMWLNDSLADGWLSVLPMAKWLQDHFI
                     EPLRGLRSTIFSV"
     gene            complement(1461..2555)
                     /locus_tag="VIR04_04135"
     CDS             complement(1461..2555)
                     /locus_tag="VIR04_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459969.1"
                     /translation="MLRTTRLVATCAAAFAVISGTALAQVPPGYPASYQQLIDAAKKE
                     GKVVVYSTTDTKAATPVVKDFEALYPGIQVEYNDLNSTELYSRYISEQAAGGSSGDVI
                     WSSAMDSTMKMATAFALTYKSVEIAKLPKWAVWKDAAYGTTYEPAAFIYNTRLITKDE
                     VPTSHKALAKLVSANAGKFKNKVTTYDVEKSAVGFMLAVQDSRNDSSYFDDLKKIGAG
                     GLVLQSSTGTMMERVSSGENLIGYNILGSYAEARAKKDPSIGVAYPTDYTLVLSRVAF
                     ISKSAKNKNAAKLWMDYLLSKRGQDILANKSSLASVRDDIDGDNDVDGMTKKLGKSLR
                     PIPVDESLLDYLQQDKRLAFIKQWRAATGK"
     gene            complement(2627..>3483)
                     /locus_tag="VIR04_04140"
     CDS             complement(2627..>3483)
                     /locus_tag="VIR04_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9459970.1"
                     /translation="AAAAKLGSVDVLINSAVDLQIKLANDGYAMHYKSPYAAELPPWA
                     VWRSEVFGFTFEPAVMVYNTKRFTDQTAPRSRQALLRLLEQDSASLYGKVGTYDISAS
                     SVGYLLASQDDLASSNFWGLANAMGEVNVQLYASSAAILDNIEQGKLDLGYNVLGSYA
                     LARQAAGHPIGIVIPQDYVLVLARSALIARSAKHPEQARAFIDWLLSPQGQIIVNSQA
                     GLGAVMPDSAQSPTRESLLKSSQGIVQPIALNPTLLVGLDRQRHDRFIQNWKRVITDT
                     RKKPPTGH"
BASE COUNT          747 a          971 c         1058 g          707 t
ORIGIN      
        1 acgctgaaga tggtggagcg taaacctcga agcggctcaa tgaagtggtc ttgcagccac
       61 ttcgccatgg gaaggacgga cagccagcca tcggccagtg aatcgttcag ccacatgggt
      121 acgaatagga agacccacaa aaacgccagg ccggccagca gtccgggtac cgcgcgcggg
      181 accagcacac tgtagtccag gaagcgcgta acgccgtcgg gtttgcgatg catggccagt
      241 ccgatggcgg tgtagcagac aactgccagc gcgccgccaa ttacgccgat ggcgactgaa
      301 ttgacgatgg cgcgcattag cgtagcttgt tcccaaatgg tgcgaaacgc ctgcaaggac
      361 agcacatcga acagcgatac gcccatgccc cagtgggaaa cgaacgagcg cagtaaaacg
      421 ccggttaacg gcacgaccac cgcgacgaac aaccaaagcg caacgatgat ccccgccggc
      481 cagcgccatt tgcccagcgg cagaggtcgt gcccgcgagg ccttgccctt gagggtgacg
      541 taacgttcag ccgagcgcat caggcgccgt tgcagcatga ccagagggat cgtgatggca
      601 atcagtatca tggccacggc ggccatcagg tggtaggagg ggattcccag cttgttcgta
      661 agcttgtaca agtacgtcga catcaccagg ttaccctcgg ggtcgcccag caccagcatc
      721 aggccgaaga cctccagacc caggaaaaaa agcagtactg tggcgtacag catggccggg
      781 cgcaccatgg gcaaactgac cgaggacatg acctggaagg gtttggcgcc cgcgacgcgg
      841 gccgcctcct caacgtccga acccatgctg tgcagcgcgg aagccatgta aagataggca
      901 tacggcacat gcgtcagacc ggcgatgatg acaatgctgg ttagcgaata gacattccac
      961 gggacgaagc ccagcagctc ctgggcccat agcgagaaga atccaacggg gccggtggcg
     1021 acgacgtatc cgaatccgag caccatcgga gacacgaaca cgggcaccag aattagcggt
     1081 tcgatccagt ggcgccccgg cagatcggtg cgcgacatca gaaacgccag aatgcttccc
     1141 aaggggacgg cgatgatggc gagtccggcc gcgagaataa ggccgctttt cagcgccttg
     1201 ggaaaatcgg ggtcggcaaa aatgaaatgg aacgcatcca ggttaaaaac cttgtcgggc
     1261 atgaagaagg gcgccgacag gaaactctgg atgatgatta gcgataaggg tacataaata
     1321 gccagcgccg tgatggcgac cacagcaccg cgtggcaaag attgccactt catgcgcagg
     1381 gattgcatgg ggtagttcct gatgatgatt cgggccgcca cgggcattcg cgcccatggc
     1441 ggcctggggg ctgtggcggc ttacttgccc gtggcggcgc gccattgctt gatgaaagca
     1501 aggcgtttgt cttgttgcag gtaatcaagc aaagactcgt caaccggaat gggccgcaac
     1561 gatttgccca atttcttggt cattccgtca acgtcattgt ctccgtcgat gtcgtcgcgc
     1621 accgacgcca ggctggactt gttggccaag atgtcctggc cgcgtttcga caggaggtag
     1681 tccatccaca atttggcggc gtttttgttt tttgccgact tgctgatgaa cgcgacacgc
     1741 gacagaacca atgtataatc ggtcgggtag gcgacgccaa ttgatggatc tttcttggcg
     1801 cgggcctcgg cgtaagaccc aagaatgttg tagccgatca gattttcgcc cgaagaaacc
     1861 cgttccatca tcgtgcccgt tgacgattgc agaaccaggc cgccggcgcc gatttttttc
     1921 aaatcgtcaa agtagctcga atcgttgcga ctgtcttgca cggccagcat gaatccgacg
     1981 gcggatttct cgacatcgta ggtcgtaacc ttgtttttga actttcccgc gtttgcgctg
     2041 accagttttg caagcgcctt gtgcgaggtc ggcacttcgt ccttggtgat caggcgcgtg
     2101 ttgtagatga aggctgcggg ttcgtaggtg gtgccgtagg cggcgtcttt ccacacggcc
     2161 catttgggca atttggcgat ctcgacggat ttataagtaa gcgcgaaagc agtggccatt
     2221 ttcatggtgg aatccatggc tgaactccag atcacgtctc cgctggagcc gccagcggct
     2281 tgttcgctga tgtaacggct gtatagctcg gtgctgttca agtcgttgta ttcgacctga
     2341 atgccgggat acagcgcttc gaaatctttg accaccgggg tcgccgcttt ggtgtccgtg
     2401 gttgagtaaa ccacgacttt cccttccttt ttggccgcgt cgatcaattg ttgatagctg
     2461 gcgggatagc ccggcggcac ctgggccagg gctgtgccgc tgatgacggc gaacgcggcg
     2521 gcgcaggtcg cgaccaggcg tgtggttcgt agcatgttgt tttctccttt gggattaccg
     2581 gggggacaat gctccccatc aaccgccatg ttaggcacgc ttgctgtcag tgtcctgtcg
     2641 gcggcttctt gcgcgtatca gtaattaccc tcttccagtt ttggatgaaa cggtcgtggc
     2701 gttgccggtc caggcccacc aatagcgttg gattcagggc aatgggctgc acgatgccct
     2761 gcgatgattt aagcagcgat tcccgcgtgg gcgattgcgc ggaatccggc atgacggcgc
     2821 ccagaccggc ctgactattg acgatgattt gcccctgggg cgacagaagc caatcgatga
     2881 acgcgcgcgc ctgctcgggg tgtttcgcgc tgcgcgcaat cagggccgag cgggccagca
     2941 ccagcacgta atcctgcgga atcacaatac caatggggtg ccccgccgct tgccgcgcca
     3001 gcgcgtatga gcccagcacg ttgtacccta aatcaagctt tccctgttca atattatcga
     3061 gaattgcggc gctgcttgcg tacaactgca cgtttacttc gcccatggca ttggccagtc
     3121 cccagaaatt cgacgatgcc aggtcgtctt gcgaggccag caaatagccc acgctgctgg
     3181 cggaaatatc gtaggtgccc actttgccat acagactggc cgaatcctgt tccagcaagc
     3241 gcagcaaggc ctggcgcgag cgcggcgcgg tctgatcggt gaaacgtttc gtgttgtaga
     3301 ccatgaccgc cggttcgaag gtgaatccga atacttccga acgccacaca gcccaaggcg
     3361 gcaattcagc ggcgtagggc gatttgtaat gcatggcgta gccgtcgttg gccagtttaa
     3421 tttgcaggtc gacggcggaa ttgattaata cgtcgacgga tccaagcttc gcggcggcgg
     3481 ctt
//
LOCUS       DATCXU010000187         5852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1151709,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000187 DATCXU010000000
VERSION     DATCXU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151709"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VIR04_04145"
     CDS             complement(<1..95)
                     /locus_tag="VIR04_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY9459971.1"
                     /translation="MMKGQIAGLMRQAQQMQENMKKAQDALAEIIV"
     gene            complement(137..1945)
                     /gene="dnaX"
                     /locus_tag="VIR04_04150"
     CDS             complement(137..1945)
                     /gene="dnaX"
                     /locus_tag="VIR04_04150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY9459972.1"
                     /translation="MTYLVLARKWRPRSFETLIGQDHVVRALTNALSSGRLHHAWLFT
                     GTRGVGKTTLSRLLAKSLNCETGITATPCGVCHACTEIDSGRFVDYVELDAASNRGVD
                     EMTQLLEQAVYAPSVGRFKVYMIDEVHMLTGHAFNAMLKTLEEPPPHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKLMTAEAIVSHLQYVLGQENIGFETPALRLIAQAAGGSMRDAL
                     SLADQAIAYSAGHLTTVVVQDMLGTVDQRHLLRLLHALSAGDGAQVMQVADELASRGL
                     SYSGALGDLAVLLSRIAIEQRVPGALSKEDPLAGDIDQLVRTMQPDVVQLFYSVAVHS
                     RQELSLAPDEYAGFVMACLRMLSLVPPAPASAPGGGSSIMRENVPDPEDSGENAPEEA
                     SQDTSEGAHEGGVAEASDDVPAWEDSRQSEPDDSGPEEPVDRDDATDIMPGDAVDVAV
                     AATFSTERKKIAAPRLSDMTAAAWPALAATLPLSGLVAELAQQSEWLGSQGDRIKLRV
                     AVQSLAESPGKSRLCTVLSEYFGMAVQLNVEYGATGEDTARAVEQAQRAESQKQAEQA
                     VQADPFVQDLMNEFDAHVVADSIRPVSGNAKLDKVA"
     gene            complement(2004..2101)
                     /gene="ffs"
                     /locus_tag="VIR04_04155"
     ncRNA           complement(2004..2101)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIR04_04155"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2112..5444)
                     /locus_tag="VIR04_04160"
     CDS             complement(2112..5444)
                     /locus_tag="VIR04_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY9459973.1"
                     /translation="MSIKPPSDADVRLAAVDPVRSFLIEAPAGSGKTELLTDRILALL
                     ATVNRPEEIVAITFTRKAASEMHARVLAKLRAALGDAPSEGHKRRSWQLAQKAMRRND
                     ELGWDLLGYPARLSIRTIDSFCAHLVRAMPWLSALGGLPAVAEDAQEHYQAAAQATLA
                     MADESEAVAALIAHLDVDLRVAQNLLATMLASRDQWLPLLTAGGDVDRLLAALNHSIA
                     IALQALGERMPEGWAAALAPPVSRAAETLATAGAGTELDALRDWEGEPLGVACEDLPK
                     WQALAWVLLTKENNLRVGVNKNQGFEAKSDYKEAFVKWAKSAKEQTGTDREWVAALGA
                     IKFAPAQGYTDAQIETLRVLIKSLWLASAQLTLRFTQANEVDFIEISQRALHALGEAD
                     EPSDLLLKLDVAIRHILVDEFQDTSQSQIDLLQRLTTGWEPGDGRTVFLVGDPKQSIY
                     RFRKAEVGWFLKVQQHGLGEVPLTALRLTDNFRSQANVVAWVNGAFGKLFPQKSNPAM
                     GAIKYSESVAFNPAIDGLGMEFHPIWLPFGADGEHKDQAQAAAESIAINLIREALARH
                     PDSEHPVAVLVRARAHLGKLVHRLMRENIPCRAVELVSLKQRQVVDDLVQLTRALSHP
                     ADRLAWLSVLRSPLCGLTLESLHALFGGDHRLTVPTVLSAWLNQDEEGPLLLDPAESQ
                     RLRRVACILLDNSNDSGAVPFAAWLEQCWRRLGGPSVYASPGDLADAERLFRQIEELA
                     PYGGLNPAELEARLNQLYAAPNSPGHAVEVMTIHKAKGMEFDTVVLMGLDRKARSDSV
                     PLVRFEQNEGELLLGPIKHRILDEPDPVSSYLAEREKVRAGYETDRLLYVAATRAREQ
                     LHLIGAVSLDENGEIKMPAASSLLGRLWDHIEKPERPSMAEIMEGDSAGTASPHSGGL
                     IRMRDVAWPKIKPPAARPSGTPWQWRPQSGDEALIGTVAHAWLERMGKEGADHWPPER
                     VAENVPLISKQLSRAGVAGAAISDATRIVQETLQATLASSRGQWLLSVARAHREWSLL
                     DLSGRVSIIDLAISQESGWLVVDYKTGAPAAHEDRDSFIAHMRTRYQEQIARYCAHVS
                     ALDGRAATGALYFPRADIWIEC"
     gene            complement(5425..>5852)
                     /locus_tag="VIR04_04165"
     CDS             complement(5425..>5852)
                     /locus_tag="VIR04_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY9459974.1"
                     /translation="RLAVIDYKTGSGKIDAKSDWMRQRPVNLQLPFYSAVLASGDASV
                     AALVLARLHAKKIEFQGLADQDYGFDGLAMLAGWPDFANLTWAELMLQWRRTIEQLAQ
                     EYSSGVATNRTLHPADLDYCDVLPFLRLNEEDPRVDQAA"
BASE COUNT         1261 a         1882 c         1558 g         1151 t
ORIGIN      
        1 acaatgattt cggccagcgc atcctgggcc ttcttcatgt tttcctgcat ctgctgggcc
       61 tgacgcatca agccggcaat ctgacctttc atcatcgtga gctatcctga aaagtggtaa
      121 agggtaataa aaacgtttag gcaactttat caagcttggc gttgccggac acgggcctta
      181 tggaatcggc cactacatgg gcatcgaatt cattcatcag gtcctgaaca aaaggatccg
      241 cctgtaccgc ctgttcggcc tgtttctgac tttcggccct ttgagcctgc tcgactgcgc
      301 gagccgtgtc ttccccagtg gcgccatact caacattcaa ttgcaccgcc atgccgaagt
      361 actcggacaa taccgtgcac aagcgagact tgccaggact ttcagccaaa gattgcacgg
      421 ccacgcgcaa cttgatgcga tcgccctgcg agcccagcca ttcgctttgt tgcgccaatt
      481 cagccacaag gccgctcaag ggaagagtcg ccgccagcgc gggccacgcg gcggccgtca
      541 tatcgctcaa tctgggcgct gcgattttct tgcgctcggt ggaaaatgtc gccgctaccg
      601 cgacgtcaac ggcatcgccg ggcattatgt cagtcgcatc gtcgcgatca accggctctt
      661 caggcccgga atcgtccggc tccgattgcc gcgaatcttc ccacgcggga acgtcatcgg
      721 acgcttcggc gacgccgcct tcatgtgcgc cttcagaagt gtcttgggac gcctcttcgg
      781 gggcgttctc gccggagtct tcgggatccg gaacgttttc gcgcatgatg ctggagccgc
      841 caccgggagc agatgccgga gcaggcggca caagtgacaa catgcgcaaa caggccatca
      901 caaagccggc atactcgtcg ggcgcgagcg aaagctcctg gcggctatgc acggcaacgg
      961 aataaaacaa ttgaaccaca tccggctgca tggttcgaac caactggtcg atatcgcccg
     1021 ccagagggtc ttctttggac agggcgcccg gtacgcgttg ctcaatggct atacgcgata
     1081 acaacaccgc caggtcgccc agcgcgcccg aatatgacaa accgcgcgat gcaagttcgt
     1141 cggccacctg catcacctgc gcgccgtcgc ccgccgacaa ggcgtgcaac agccgcagca
     1201 gatgccgctg atcgaccgtg cccagcatat cctgaacgac cacagtcgtt aaatgcccgg
     1261 cgctatacgc aatcgcctga tccgcgagcg acaatgcatc gcgcatcgaa cctcccgccg
     1321 cctgtgcaat caaacgcagc gcgggggtct cgaaaccaat gttctcctgg cccagcacat
     1381 actgcaggtg cgacacaatg gcttccgccg tcatcagctt aagattgaac tgcaggcaac
     1441 gcgacaacac cgtcaccgga attttttgcg ggtcagtggt cgccagaatg aatttgacgt
     1501 gcgggggcgg ctcttcaaga gtcttcagca tggcgttaaa ggcatgcccg gtcagcatat
     1561 gcacttcgtc gatcatgtag accttgaagc ggcccacgct gggcgcatat accgcctgtt
     1621 cgagcagttg ggtcatttca tcaacgccgc ggttggatgc cgcgtcgagc tcgacgtaat
     1681 cgacaaaacg gcccgagtcg atttcagtgc acgcgtggca taccccgcaa ggagtcgcgg
     1741 tgatgcccgt ttcgcagttc agcgatttcg ccagcaatcg cgacagcgtc gtcttgccga
     1801 cgccgcgcgt tccagtaaac agccaggcgt gatgcaggcg cccgctggat aacgcgttcg
     1861 tcagcgcacg aaccacgtga tcctgcccga taagcgtttc aaacgaccgc ggacgccatt
     1921 tacgcgcgag taccaaataa gtcatgcgag aactgtgagc ccgacaggct atcgacgaac
     1981 aagtgcaaag tgtactggat tcggttggcg agcctgactt cggcactggt cctgagcgac
     2041 tatggctgct tcgttcccga cctgaccaga ttcaccgccg aaccatgcga aggggcccgc
     2101 cagaaaacag tttagcattc gatccagata tcagcacgcg gaaaatacaa ggcgccggtt
     2161 gcggcacgtc catccaacgc gctgacatgg gcgcagtaac gggcaatttg ttcctgataa
     2221 cgtgtgcgca tgtgcgcaat aaaggaatca cgatcctcgt gcgccgcagg cgctccggtt
     2281 ttgtagtcga ccaccagcca gccgctttcc tgcgaaatcg ccaagtcaat gatcgacaca
     2341 cgcccgctca aatccagcaa ggaccactcg cgatgagccc gcgcaacgct tagtaaccac
     2401 tgccctctcg aactggccag tgtggcctgg agcgtctcct gaacgattcg cgtcgcatcg
     2461 cttatagcgg cgccggccac cccggcacgg cttagttgct tgctgatcaa cggcacgttt
     2521 tcggcaaccc gttctggcgg ccagtgatca gcgccctctt tgcccatacg ttccagccag
     2581 gcatgggcaa cggtgcctat gagggcttcg tcgcccgatt gcggacgcca ctgccagggc
     2641 gtgccggacg gacgcgctgc aggcggcttt atcttgggcc aggcaacatc gcgcattcga
     2701 atcaggccgc ccgaatgcgg cgatgccgtc cccgccgaat cgccttccat gatttccgcc
     2761 atggatggcc tttcaggctt ttcaatatgg tcccacaatc gccccagcag gctgctcgcc
     2821 gccggcattt ttatttcgcc attttcatcc aggctcacgg cgccgatcaa atgcagttgt
     2881 tcacgcgcgc gagtcgccgc cacataaagc aggcgatctg tctcgtagcc ggcccgcacc
     2941 ttttctcgtt ccgccagata ggatgacacg gggtcaggct catcaaggat tcgatgtttg
     3001 atgggtccca acaacaattc gccctcgttt tgctcgaagc gcaccagcgg aacgctgtcg
     3061 gaacgagcct tgcgatccag ccccatcaag acaaccgtat cgaattccat tcccttggct
     3121 ttgtgtatcg tcatgacctc cacggcatgc ccgggactat tgggcgccgc gtacaactga
     3181 tttagtctgg cctccagctc ggctggattc agaccgccat agggcgccaa ttcctcgatc
     3241 tggcgaaaca gccgctcggc atcggccaaa tcgcccggac ttgcatagac tgaagggccg
     3301 cccagacggc gccagcattg ttcaagccag gcggcaaaag gaaccgcgcc tgaatcattg
     3361 gaattatcca gcagaatgca ggcaacccgg cgcaaacgct gcgattcggc cgggtccagc
     3421 aacaacgggc cttcttcatc ttgattcagc cacgccgaca acacggtggg aaccgtcaat
     3481 cgatgatcgc ccccaaataa agcatgcaaa ctttcaaggg tcaaaccgca aagcggcgaa
     3541 cgcaaaactg aaagccatgc caggcggtca gccggatgcg acagcgcgcg cgtcaactgc
     3601 acaagatcat caaccacctg gcgttgcttg agcgaaacca gctcgacagc gcggcaggga
     3661 atattctccc gcatcaggcg atgcacaagc ttgccaagat gagcgcgcgc acgcaccagg
     3721 actgctacag ggtgttcact atcggggtgt cgtgccagcg cttccctgat caggttaatg
     3781 gcaatcgatt cagccgccgc ctgcgcctga tccttgtgtt ctccatcggc gccgaacgga
     3841 agccagatcg gatgaaactc catccccaac ccgtcgatag ccggattgaa cgccacggac
     3901 tcggaatact taattgcccc catggccggg ttggattttt gcgggaacag cttcccaaac
     3961 gcgccattca cccaggcgac cacattggcc tgcgagcgaa agttatcggt cagcctcaaa
     4021 gcggtcagcg gcacttcgcc caaaccgtgc tgctgcactt tcaggaacca cccgacctca
     4081 gccttgcgaa accggtaaat ggactgcttc gggtcaccga ccagaaacac agtgcggccg
     4141 tcacccggtt cccaacccgt cgtcagccgc tgaagcagat cgatttgcga ttgactggta
     4201 tcctggaatt cgtccaccaa tatatgcctg atcgccacat ccagcttcag gagcagatcg
     4261 ctgggttcgt cggcctcgcc cagcgcatgc aaggccctct gggagatttc gatgaagtcc
     4321 acctcgttgg cttgcgtaaa acgcaaggtc aattgcgccg acgccaacca tagcgacttt
     4381 atcagcaccc gcaaggtttc aatttgcgcg tccgtgtaac cctgcgccgg cgcaaacttg
     4441 atggcgccca aggcggcaac ccattcccga tccgtgcccg tttgctcttt ggcgctcttg
     4501 gcccacttta cgaatgcctc tttatagtcg gatttggctt cgaacccctg gtttttattg
     4561 acgcccaccc gcaagttatt ctctttcgtc aacaacaccc aggcgagcgc ctgccatttg
     4621 ggcagatctt cgcaggcaac acccaacggc tcgccttccc agtcgcgcaa tgcgtccagc
     4681 tccgtcccgg cgccggctgt cgccagcgtt tcggcggcgc gcgaaaccgg cggcgccaaa
     4741 gccgccgccc acccctcggg catcctttca ccaagcgcct gaagagcaat tgcaatcgaa
     4801 tggttcaacg cggccagcaa ccgatcaaca tcaccgcccg ccgtcaatag cggcaaccat
     4861 tgatcacgac tggccaacat tgtggccaac aagttttggg caacacgcag gtcgacatca
     4921 aggtgcgcaa tcaaggcggc caccgcctcg gactcatccg ccatggccag cgttgcttgc
     4981 gccgccgcct gataatgctc ttgtgcatct tcggccacgg cgggcaggcc gcccagcgcg
     5041 cttagccacg gcatggcacg caccaggtgg gcgcaaaacg aatcgattgt gcgtatgctt
     5101 aaacgagccg ggtagccaag caaatcccag cccaattcat cgttgcgccg catggccttt
     5161 tgcgccagtt gccaactgcg ccgtttgtgg ccctctgaag gcgcatcgcc caaagcggcc
     5221 ctcagcttgg cgagcacacg cgcatgcatt tcggaggccg ccttgcgggt gaaagttatc
     5281 gcgacaattt cctcgggccg attcaccgta gccagcagag ccagaatgcg atccgtcaat
     5341 aattcggtct ttcccgaccc ggccggcgct tcgatcagaa acgaccggac cggatcaacc
     5401 gcggcaagcc gaacgtctgc atcactaggc ggcttgatcg acacgcggat cctcctcgtt
     5461 caggcgtaaa aaaggcaaca cgtcgcaata atccagatcg gcaggatgca gagttcgatt
     5521 tgtcgccaca cccgatgaat attcctgcgc caactgctct atggttcgac gccactgcag
     5581 cattaattca gcccatgtca aattggcaaa gtccggccaa ccggcaagca tagccaggcc
     5641 atcaaaacca tagtcctggt ccgccagccc ctgaaattca atttttttcg catgcagtct
     5701 ggccaaaacc aaagcggcca cgcttgcatc tcccgacgcc aaaactgccg agtaaaaagg
     5761 aagctgcaaa tttacgggcc gctgacgcat ccagtccgac ttcgcgtcga tttttccgct
     5821 tcccgttttg taatcaatca cggccaggcg tc
//
LOCUS       DATCXU010000188         7478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_317182,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000188 DATCXU010000000
VERSION     DATCXU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317182"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VIR04_04170"
     CDS             complement(<1..609)
                     /locus_tag="VIR04_04170"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY9459975.1"
                     /translation="MNKPAYPHPVIAREGWPFLAGMALVSIIIAFWSLLWSLPFFLLF
                     LFMLQFFRDPARLGPAGEFNVLSPADGRVMAVERIKDTYAERDALKISVFMNVFNVHS
                     NRSPVDGEVLAVNYFAGKFFNASLDKASLENERSAMILRTVHGHIITAVQVAGLVAKR
                     ILCYAKPGDQLYAGQRYGFIRFGSRVDVYLPTGSRPRVAIGDK"
     gene            complement(696..1712)
                     /gene="ilvC"
                     /locus_tag="VIR04_04175"
     CDS             complement(696..1712)
                     /gene="ilvC"
                     /locus_tag="VIR04_04175"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY9459976.1"
                     /translation="MKVFYDKDCDLSLIKGKKVAIIGYGSQGHAHALNLHESGVNVVV
                     GLRKGGASWSKAENAGLKVMEVDQSVKGADLVMMLLPDENIGEVYRNHVAQNIKAGAA
                     LAFAHGFNIHYAQVIARPDIDVIMIAPKAPGHTVRSTYAQGGGTPSLVAVAQDVSGAA
                     RDVALSYACAIGSGRAGVIETTFREETETDLFGEQAVLCGGTVELIKAGFATLVEAGY
                     APEMAYFECCHELKLIVDLIYEGGIANMNYSISNNAEYGEYESGPKIIGAASKQAMRA
                     VLDDIQSGDYAKRFILENAAGAPGLTSRRRINAELQLEQVGGKLRAMMPWIAANKLVD
                     KTKN"
     gene            complement(1786..2277)
                     /gene="ilvN"
                     /locus_tag="VIR04_04180"
     CDS             complement(1786..2277)
                     /gene="ilvN"
                     /locus_tag="VIR04_04180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY9459977.1"
                     /translation="MKNIISILLENEPGALSRVVGLFSARGYNIETLTVAPTEDLTLS
                     RMTIVTEGSAEVIEQITKHLNRLVDVVKVVNLSEGPHIERELMLIKVRAVGKEREEMK
                     RMAEIFRGRIIDVTDKVYTIELTGVQEKIAAFIDALDRSAILETVRTGVSGIGRGERV
                     LKL"
     gene            complement(2288..4009)
                     /locus_tag="VIR04_04185"
     CDS             complement(2288..4009)
                     /locus_tag="VIR04_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEY9459978.1"
                     /translation="MELNGADIVVRCLIEEGVDHVFGYPGGAVLYIYDAIYKQDKFQH
                     VLVRHEQAAVHAADAYSRSSDKVGVALVTSGPGVTNAITGIATAYMDSIPLVVISGQV
                     PSYAIGEDAFQECDTVGITRPCVKHNFLVRDVKDLARTMRRAFFIAKTGRPGPVLVDI
                     PKDVSMAPCKFTPRPAEMHMRSYAPVVKGHQGQIKKAVQILLAAERPMIYSGGGTVLS
                     DASAELRQLVELTGAPCTTTLMGLGAFPASSDKFVGMPGMHGTYEANMAMQNCDVLIA
                     VGARFDDRVIGNTKNFAQNPRKIVHIDIDPSSISKRVRVDVPIVGNVKDVLLDLNGLY
                     AQAAGQVSANKPALAKWWQQINVWRGKQCLKYAGSDTVIKPQFVIEKLWEVTGGNAFV
                     TSDVGQHQMWAAQYYRFDEPRHWINSGGLGTMGVGLPYAMGVQMAHPGCDVAVITGEG
                     SIQMNIQELSTCKQYHLTPKVLCLNNRYLGMVRQWQQIDYGARYSESYVDALPDFVKL
                     VESYGHVGMRMEKPADVEPAIREAFTKHKDRLVFMNFITDQTENVWPMVKSGRGLTEM
                     LLGSEDL"
     gene            complement(4399..5085)
                     /locus_tag="VIR04_04190"
     CDS             complement(4399..5085)
                     /locus_tag="VIR04_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY9459979.1"
                     /translation="MITDFESFKVELSLSKADICRELYRRNTDLIRIKKEPVAVKNLG
                     RIIDSTLRLAASKGFHAMSLRDLCADAGFSVGGLYAYIKNKDDLIRLIQHHGFIMTRR
                     TMLAYTSGIDSSRDKLFAAIKTHLYLSELMPTWFCFSFMEAKGLPDKEKKEAIAIEQE
                     IEDIFFDIVQQGISAGVFKAVNARLLAAIIKAMMQDWYLKRRKYRNQGVTVNHYAEVV
                     RDILESYLLQ"
     gene            complement(5099..5806)
                     /locus_tag="VIR04_04195"
     CDS             complement(5099..5806)
                     /locus_tag="VIR04_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459980.1"
                     /translation="MTILELNEIHTYYGESHALQGVSLQLEEGETVCLLGRNGAGKST
                     TLKSIIGLTPPREGRVVFDGHDVARLPAYRIARMGVGLVPEDRRIFPGLSVRENLEIA
                     QYRRPGVVPQWSVERIFDEYPMLSELANQDGHTLSGGQQQMLAVARSLMSEPRLLLLD
                     EPNEGLAPVIVRQIGELIDQLSKSTTILFTDQSVHFALKHAQRAYILEKGRIVHVARS
                     QDLVQDTDTQHRYLSVA"
     gene            complement(5803..6570)
                     /locus_tag="VIR04_04200"
     CDS             complement(5803..6570)
                     /locus_tag="VIR04_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9459981.1"
                     /translation="MNEPVLRISNLSRHFGGVAAVSGITLDVMHKETLAIIGPNGAGK
                     TTLYNMLSGRMVPTTGKIIFEGHDITGLPPHRISRLGVSRSFQINNIFTEMTVRENVE
                     VALTAYHGDSRRWNNIASHNRPIHKEADELLDRLSITALGTQRAGVISYGDKRLVEIA
                     VVLATRPRLVLLDEPTAGMTPDETHRVTNLIKSLAQSGDYTFLVTEHDMQVVFDVADR
                     ILVMHHGAMLALGSPQQVKADPEVRRAYLGDAEEEIV"
     gene            complement(6567..>7478)
                     /locus_tag="VIR04_04205"
     CDS             complement(6567..>7478)
                     /locus_tag="VIR04_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9459982.1"
                     /translation="AVLLGLLALPWAAPYFYVFIVTRILIMGLFAASFNLIFGYTGML
                     SFGHAAFFGAGAYATAFLLQGLQSPLLLCLLAAMAAGALLALIIGFFSVRLDEVYFSM
                     LTLAFGMMVFAIVYQWRSVTNGSDGIAGFRVGSFGLGIDLALGNPSVYYYVVLGVVLI
                     ASALLYLICRSSFGLILRAIRQNPERVAFCGLDVQRYRLAAFTIAGAFAGLAGGLMAP
                     FLRIASPEMAHWSMSAEPVLMSILGGTGYFLGPFFGAGMFVLLETWITSMTQAWMLVL
                     GIILALMVMFFRRGVLGTFLDWWLERK"
BASE COUNT         1856 a         2138 c         1864 g         1620 t
ORIGIN      
        1 tttgtcgccg atagcgaccc gcgggcgtga gccggtcggc aaatagacat cgacgcgcga
       61 accaaatctg ataaagccat agcgctggcc tgcgtatagt tggtcgcccg gcttggcata
      121 acaaaggata cgcttggcta caaggcctgc gacctgcacg gccgtaataa tatgaccgtg
      181 aaccgtgcgc aatatcatcg cactgcgctc gttttcgagc gatgctttat ccagcgatgc
      241 gttaaaaaat ttacccgcga aataattgac ggccagcact tcaccatcga cgggcgaacg
      301 gttcgagtga acattgaaaa cgttcatgaa cacactgatc ttgagcgcgt cgcgttcagc
      361 atacgtgtct ttaattcgct cgaccgccat cacacggcca tcagcgggtg acagcacatt
      421 gaattcacct gcggggccaa ggcgtgccgg gtcgcggaaa aactgcaaca tgaacaaaaa
      481 cagcaagaaa aacggcagcg accacaacaa agaccagaac gcaataataa tggaaacaag
      541 cgccatgccg gccagaaaag gccagccttc gcgcgcaatg acagggtggg gataagcagg
      601 tttattcatc gtaagacgaa gaataactca tttggtccca tctgatatgg gcgacgccgc
      661 taccgcaaac cacggtagcg gcgctcgttt ggcgcttagt tcttggtttt atccaccagc
      721 ttgttagccg caatccaggg catcatcgca cgcaacttgc cgccaacctg ctcaagctgc
      781 aattcggcat tgatgcgccg gcgcgatgtc aaaccgggag cgccggcggc attttcaaga
      841 atgaaacgct tggcgtaatc acccgattga atatcgtcca gcacagcgcg cattgcctgc
      901 ttgctggccg cgccaatgat ttttggaccg ctttcgtact caccatactc ggcattgttc
      961 gaaatggaat aattcatgtt ggcaatgccg ccctcgtaaa ttaaatcgac aatcagcttg
     1021 agctcgtgac aacattcaaa ataggccatt tcgggcgcgt agcccgcctc aaccagggtg
     1081 gcaaaaccag ccttgatgag ttcgaccgtg ccgccgcata gaacagcctg ttcgccgaac
     1141 aagtcggtct cggtttcttc acggaaggtg gtttcaataa cgccggcgcg gccgcttccg
     1201 atagcgcaag cataggacag tgcaacatca cgcgcggcac ccgacacatc ttgcgccacg
     1261 gccaccagcg aaggcgtacc gccgccctgg gcgtaagtgc ttcgcaccgt atggcccggc
     1321 gccttgggcg ctatcatgat gacgtcgata tcggggcgcg caataacctg agcatagtga
     1381 atgttaaagc cgtgagcaaa ggccagcgct gcgcctgcct tgatgttttg ggcaacgtga
     1441 ttccgataaa cttcgccgat attttcatcg ggcagcagca tcatgaccag atcggctccc
     1501 tttaccgatt gatccacttc cataaccttc aggcccgcat tttcagcctt gctccatgaa
     1561 gcaccgcctt tacgcagccc cacaacaacg ttgacgccgg actcatgcaa attcaacgca
     1621 tgggcgtggc cttgcgagcc atagccaatg atggcaacct ttttgccttt aattaaagaa
     1681 agatcgcaat ctttgtcata aaaaactttc atttatcgct ccagtaaggc gggattttca
     1741 agttgaacct gaacgtatgg cgtccggttt tataagtaaa gtgaattaaa gctttaagac
     1801 tcgctcgccg cgaccaatgc ccgatacgcc agtgcgcact gtttccagga tggcgctgcg
     1861 atccagcgca tcgataaaag ccgctatttt ttcttgcaca ccggttaatt cgatggtgta
     1921 caccttgtcg gtcacatcaa tgatacggcc acggaaaatt tccgccatgc gtttcatctc
     1981 ttcgcgctct ttgcccacag cccttacctt gatcagcata agttcgcgct cgatatgagg
     2041 gccttcggac aaattcacca ctttgaccac atcgaccaga cgattcaaat gtttggtgat
     2101 ctgctcaatg acctcagccg agccttctgt gacaatcgtc atgcgcgaca gggtcaaatc
     2161 ctcggtgggc gcaaccgtca aggtttctat gttatagccg cgcgccgaaa acaaacccac
     2221 aacccgggac aaggcgcctg gttcgttttc cagaaggata gaaataatgt ttttcatttt
     2281 cctttcctta tagatcttca gagccaagca acatctcggt cagtccgcgg cccgatttca
     2341 ccatgggcca tacgttttct gtctgatccg taatgaaatt cataaacacc agacggtcct
     2401 tatgcttggt aaaggcctcg cgaatagccg gttctacgtc ggccggcttt tccatacgca
     2461 tgccgacatg cccatagctt tcaacgagct tcacgaaatc gggcaaggcg tcgacgtaag
     2521 attccgaata acgcgcgcca tagtcgattt gctgccactg acgcaccatg cccagatagc
     2581 gattattcag gcacagcact tttggcgtca ggtgatactg cttacaggtg gagagctcct
     2641 gaatattcat ctgaatagag ccttcacccg taatgacggc gacatcgcaa ccgggatgcg
     2701 ccatttgcac ccccatggca tagggcaagc ccacgcccat ggttcccagg ccgcccgaat
     2761 taatccagtg gcgtggctca tcgaagcggt agtactgcgc tgcccacatt tgatgctgcc
     2821 ccacatcgga cgtcacgaaa gcgtttccac ccgtgacttc ccacaacttt tcgatgacaa
     2881 actgaggctt gataacggtg tcggaacccg catatttcag gcattgcttg ccgcgccaga
     2941 cattgatttg ttgccaccac ttggccagcg ccggtttatt ggccgaaacc tgccccgctg
     3001 cctgggcata caagccattc aaatcaagta aaacgtcttt aacattgccg acgataggca
     3061 cgtcgacccg cactcttttg gaaatcgatg aaggatcaat atcaatgtgt acgatcttgc
     3121 gcgggttctg cgcgaagttt ttcgtgtttc cgataacccg atcatcgaat cgcgcgccga
     3181 cggcaatcag aacatcgcaa ttctgcatgg ccatgttggc ttcgtacgtt ccgtgcatgc
     3241 ccggcattcc gacaaacttg tcgctactgg ccgggaacgc gcccaggccc atcagggtgg
     3301 tggtgcacgg cgcgccggtc agttccacca gttgccgcaa ttcagccgac gcgtcggaaa
     3361 gcacagtgcc gccgcctgaa taaatcatgg gccgttccgc ggccagcaag atctgcaccg
     3421 cctttttaat ttgcccctga tgccctttga ctaccggcgc atacgaacgc atatgcattt
     3481 ctgccggcct gggagtaaat ttgcacggcg ccattgacac gtctttgggt atatcgacga
     3541 gcacggggcc cggacggccc gttttggcga tgaagaaagc gcggcgcatg gttcgggcca
     3601 gatctttcac atcgcgcact aaaaagttgt gcttgacgca aggccgggta atgcccacgg
     3661 tatcgcattc ctgaaatgca tcttcgccga tggcgtagct gggcacctgt ccgctaatga
     3721 ccaccagggg aatggagtcc atataggcgg tggcaatacc cgtaatggca ttggtcacgc
     3781 ccgggccgct ggtcaccaat gcaacgccaa ctttgtctga agagcgcgaa taggcatcgg
     3841 ccgcatgcac agccgcttgt tcgtggcgca ctagaacatg ctggaatttg tcttgtttgt
     3901 aaatggcatc gtaaatatac aggaccgcac caccggggta accaaataca tggtcaacac
     3961 cctcttcgat caggcaacgc acgacgatat cggcgccatt gagttccatg ttgtattcct
     4021 ttcgtaaccg gcactcatat ttcgatcggc cgtcaaagcc tgagccaaat cgattccgac
     4081 aacatgatct gacgctttgt ggctcacgga gccacgccgc ccagacacct tgccgatccg
     4141 acttgcactc gtcgaaacac agtgcgagcg ttcatcacga acgcgggagg tagaaacgga
     4201 agcttcggca acacacgctt atggcgcggc cagcagcaaa taaaccaagc gcaaaaaacc
     4261 gggcggatgg cccggacgac agcacatttg gcagcccacc aaggataacc gaatgggctg
     4321 aagctgtact ttaccgcgtt gcaccatcag aatcaactcg gcgagcaatc cagtctgaac
     4381 cggatccgcc cgagccggct attgcaaaag atagctttcc aggatgtctc tgacaacttc
     4441 tgcgtagtga ttgaccgtca cgccctgatt gcgatatttg cggcgcttca aataccagtc
     4501 ttgcatcatt gccttgatta tcgcggcgag caaacgagca ttgaccgctt taaaaacgcc
     4561 ggctgatatt ccctgctgaa cgatatcgaa aaaaatatct tcgatttcct gctctatggc
     4621 gatagcttct ttcttttctt tatccggcag accttttgct tccataaagg aaaagcagaa
     4681 ccaggtcggc atgagctcgc taagatataa atgcgtttta atcgccgcga ataacttatc
     4741 gcgtgatgaa tcgatgccgc tggtataagc cagcatagtg cgccgggtca taatgaagcc
     4801 atgatgctgg atcaaacgaa tcagatcgtc tttatttttt atataggcgt acaaccctcc
     4861 aacgctaaac cctgcatcgg cgcataaatc acgcaaggac atggcatgaa aacccttcga
     4921 cgcggccaga cgcaatgttg aatcaataat tcgacccaga ttcttcaccg caacgggctc
     4981 tttttttata cggattaaat ctgtgttccg acgataaagt tcacggcaaa tatcggcctt
     5041 ggacaagctg agttcgacct tgaacgattc gaaatcggta atcatggaaa gccgctcatc
     5101 aagccaccga gagatagcga tgttgagtat cggtatcctg aacaaggtcc tgactacggg
     5161 caacatgcac gattcggccc ttttccagaa tataggcgcg ttgtgcgtgt ttcaacgcga
     5221 aatgcacgct ttggtcggtg aataaaatcg tggtcgattt tgacaactga tcgatcagct
     5281 cgccgatttg cctgacgatg actggggcca gaccttcatt gggctcatca agaagcaaca
     5341 agcgcggttc gctcattagc gagcgcgcta ccgccagcat ttgctgttgt ccgcccgata
     5401 gggtatggcc atcctggttc gccagttcgc tcagcatggg gtattcgtca aaaatacgtt
     5461 cgaccgacca ttgcggcacg accccaggtc gccgatattg tgcaatttcc aaattttcac
     5521 ggacgctcaa tccagggaaa attcgccggt cttcgggcac caggcccaca cccatgcgcg
     5581 caatgcgata tgcgggcagc ctggcaacat cgtgcccgtc gaacaccaca cgcccttcgc
     5641 gcggcggagt caggccaata atgcttttta acgtcgtgct ttttccggcg ccattgcgcc
     5701 caagcaaaca taccgtctcg ccctcttcaa gctgtaaaga caccccttgc aatgcatgac
     5761 tttcgccgta ataggtgtga atttcgttca attccagaat cgtcatacga tttcctcttc
     5821 ggcatcgccc aaataggcgc gcctgacctc gggatccgcc ttcacctgtt gcggcgaacc
     5881 cagagccagc attgcgccat ggtgcatgac caaaatacga tcggcaacat cgaaaaccac
     5941 ctgcatgtca tgttcggtca ccaagaatgt gtagtcgcca ctttgcgcga gcgatttgat
     6001 caaatttgtg acgcgatgcg tttcgtcggg agtcattccc gctgtgggct cgtcaagcaa
     6061 caccaggcgc ggccgggttg caagcacgac ggcaatttca accagacgct tgtcgccgta
     6121 actgatcact cccgcccgct gcgtgcccaa ggcggtaatc gacaagcgat caagcagctc
     6181 gtcggcttct ttgtggatcg gccgattatg cgaggcgata ttgttccagc gacgactgtc
     6241 gccgtggtac gcagttagcg ccacttcgac attttctctg accgtcattt cagtgaatat
     6301 attgttgatc tgaaacgacc gggagacccc aagccggcta atgcgatgcg gcggcaagcc
     6361 cgtgatgtcg tgtccttcga atatgatctt gcccgttgtc ggaaccattc ggcccgacaa
     6421 catgttgtag agcgtggttt tgccggcgcc attggggccg ataatggcca gcgtttcctt
     6481 gtgcatgaca tccagagtaa tccccgaaac agcggcgacg ccgccgaagt gcctggataa
     6541 attgctgatc cttaatacgg gttcgttcat tttcgttcca accaccaatc aagaaaggtt
     6601 cccagcacgc cgcgccggaa aaacatcacc atcagcgcaa ggataatgcc cagcaccagc
     6661 atccaggcct gggtcatgct ggtgatccag gtttccagca acacaaacat tccagcgcca
     6721 aaaaatggcc cgaggaaata gcccgtaccg cccaatatgg acatcagcac aggctcggca
     6781 gacattgacc agtgcgccat ctcggggctg gcgatgcgta aaaaaggcgc catcaggcca
     6841 cccgcaagtc ccgcgaaagc gcccgcaatg gtaaaggcgg ccaggcgata gcgctgaaca
     6901 tcaaggccgc aaaacgccac ccgctcgggg ttttgccgaa tagcgcgcaa aatcaggcca
     6961 aacgaagaac ggcaaatcaa atacagtagg gctgacgcga tcagcaccac gcccagcacc
     7021 acatagtaat aaacgctggg attgcccaag gccagatcga tccccaatcc gaatgaaccg
     7081 acgcgaaagc ccgcgatgcc atcgctgcca ttggtcacgg agcgccattg gtacacgatg
     7141 gcaaacacca tcatgccaaa cgccagggtc agcattgaaa aataaacttc gtccaggcgc
     7201 acgctaaaaa atccgataat cagggccaac aatgcgccag ccgccattgc agcgagcaag
     7261 caaagcaaca aaggactctg caggccttgc aggagaaacg ccgttgcgta cgcgcccgct
     7321 ccaaaaaagg ctgcatgccc gaaactgagc atgcccgtat agccgaaaat aagattaaag
     7381 ctggcggcaa acaaacccat gatcagaatt cgggtgacga taaacacgta aaaatagggt
     7441 gcggcccaag gcagcgcaag caagcccagc aaaacagc
//
LOCUS       DATCXU010000189         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1145064,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000189 DATCXU010000000
VERSION     DATCXU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145064"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VIR04_04210"
     CDS             <1..359
                     /locus_tag="VIR04_04210"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEY9459983.1"
                     /translation="DVLTGISLVRGQAFDFPLAITMLERHLLGSQSWMSLNHTPFVAV
                     VAPNGADGRPDESRIRAFYVEGNQGVNYHPGAWHHPLLALGEQGDFIVVDRISATPDC
                     DICDLGQSYLIDGSFC"
     gene            450..>2487
                     /gene="alaS"
                     /locus_tag="VIR04_04215"
     CDS             450..>2487
                     /gene="alaS"
                     /locus_tag="VIR04_04215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY9459984.1"
                     /translation="MKTTEIRQKFLSFFESKGHQRVASSPLVPANDPTLLFTNAGMVQ
                     FKDVFTGKETRPYKRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIHYAWELLTSVYKLPADKLWVTVYQEDDEAYDIWANEIGVPVERIVRIGDNKGAR
                     YASDNFWQMADTGPCGPCSEIFYDHGPEIFGGPPGSPDEDGDRYIEIWNLVFMQFERD
                     AAGNMPLLPKPCVDTGMGLERIAAVLQHVHSNYDIDLFQNLIKAAARETGTRDLNNNS
                     LKVIADHIRACSFLIVDGIIPGNEGRGYVLRRIVRRALRHGHKLGQTQVFFYRLVADL
                     VKEMGAAYPELAHQQARVEQVLKQEEERFRETLEHGMKILDSALAGVPENGVLDGQTL
                     FTLYDTYGFPVDLTADICRERKVEVDLEGFDAAMGRQRDQARAAGKFKAAEGLSYHGA
                     KTRFDGYEHLQGDGKVTALYVEGTSVDAISEGQQAVVVLDATPFYAESGGQVGDRGEL
                     LAGNVRFEVSDTRKIQSGVFGHHGRLVSGSVAVGDVISAHVDTQRRARTMRNHSATHL
                     MHRALRQVLGEHVQQRGSLVDPEKTRFDFAHDAPLSAAQIAQVEQIVNAEVLANQEVS
                     TRIMAYDQAVQSGAMALFDEKYGDTVRVLDIGFSRELCGGTHVRRTGDIGLFKIV"
BASE COUNT          533 a          651 c          757 g          546 t
ORIGIN      
        1 acgacgtttt gacgggcatc agcctggtgc gcggccaggc atttgacttc ccgttggcaa
       61 tcacgatgct cgagcggcat ctgctgggca gccaatcatg gatgtcactg aaccatacgc
      121 cctttgttgc ggtcgtggcg ccaaatggcg ccgatggccg gcccgacgaa tcgcgcatca
      181 gggcgttcta tgttgaagga aaccagggcg tcaattacca cccgggtgcc tggcaccatc
      241 ccttgctggc gctgggcgag cagggcgatt ttatagtggt cgaccggatc agcgccacgc
      301 cggattgcga catatgcgat ttaggtcaat cgtatctgat cgacggttct ttttgctgat
      361 caggcggcgg cgcgggcggc cgggcgcagc gtatttcccc caacagcctt tataatcgac
      421 cgttatttat caattagtgc ttgcgcgcaa tgaaaacgac agagatacgt caaaaatttt
      481 tatcattctt tgaatccaaa ggacatcaac gggtggcctc atcgcctctg gtgcctgcca
      541 atgaccccac tttgctcttt acgaatgcgg gaatggtgca gtttaaagac gttttcaccg
      601 ggaaggaaac acgcccgtac aagcgggcaa ccagctcgca acgctgcgtg cgcgcgggcg
      661 gcaagcacaa cgatctggaa aatgtaggct acacggcgcg gcaccataca tttttcgaga
      721 tgctcggcaa cttcagcttt ggcgattatt tcaagcgcga tgccattcat tacgcctggg
      781 aactgctgac ctcggtgtac aagttgccgg ccgacaaact atgggtgacg gtttatcagg
      841 aagacgacga ggcgtacgac atatgggcca atgaaattgg cgtgccagtc gaacgaattg
      901 tccgcatagg cgataacaag ggcgcgcgct acgcctctga caatttttgg caaatggccg
      961 atacagggcc ttgcggccct tgttcagaga ttttctacga tcatggtcct gaaattttcg
     1021 gcggccctcc gggatcgccc gacgaggacg gcgatcgcta tatcgagatc tggaatctgg
     1081 tgtttatgca attcgagcgc gacgcggcgg gaaatatgcc gcttttgccc aagccttgcg
     1141 ttgacacggg aatgggtttg gagcgtattg cggcggtctt gcagcatgtg cattccaatt
     1201 acgacatcga cctgtttcag aacctgatca aggcggcggc gcgcgaaacc ggtacgcgcg
     1261 acctgaacaa taattctctg aaagtgattg ccgatcatat tcgcgcctgc agcttcctga
     1321 ttgtcgacgg catcattccc ggcaatgaag ggcgcgggta tgtgttgcgt cgcattgtgc
     1381 ggcgtgcatt gcgtcacggc cacaagctgg gccagaccca ggtgttcttt tatcgtttgg
     1441 tggccgacct ggttaaagaa atgggcgcgg cgtatcccga attggctcat cagcaggcgc
     1501 gtgtcgagca ggtccttaag caagaagaag aacgctttcg cgaaacgctt gaacatggca
     1561 tgaaaattct ggactccgct ttggccggtg tgcccgaaaa tggcgtgctc gatggccaga
     1621 ccttgttcac gttgtatgac acctacggct ttcccgttga cctgaccgct gatatttgcc
     1681 gcgagcgcaa ggttgaggtc gatctggaag ggttcgatgc ggccatgggg cgccagcgtg
     1741 accaggcgcg cgccgcaggc aagttcaagg cggccgaagg gcttagctac catggggcca
     1801 aaacgcgttt cgatggctat gaacatctgc agggtgatgg gaaagtgacc gccttgtatg
     1861 tcgaaggcac ttcggttgac gccatctcgg aaggtcaaca ggcggtggtt gtgcttgatg
     1921 cgacgccgtt ttatgcggaa tcgggcgggc aagtgggcga taggggcgaa ctgctcgccg
     1981 gcaatgtccg tttcgaagtg tccgatacgc ggaagataca aagcggcgta tttggccatc
     2041 acggcagatt agtcagcggc tcagtcgcag taggcgatgt gatcagcgcg catgtcgaca
     2101 cgcagcggcg cgcccgcacg atgcgcaatc attccgcaac tcatttaatg cacagggcgt
     2161 tgcgtcaagt attgggcgag catgtgcagc agcgcgggtc gttggtcgat cccgaaaaga
     2221 cccgcttcga ttttgcgcac gacgcaccct tgtcggcagc gcaaatcgcg caggtcgagc
     2281 agatcgtgaa tgccgaagtc ttggcgaacc aggaggtcag cacgcgaatc atggcttacg
     2341 atcaagccgt ccaaagcggc gcgatggcat tgttcgacga aaaatacggc gatacggtgc
     2401 gggtgcttga tatcggcttc tcgcgcgaat tatgtggcgg cacgcacgtg cgccgcacag
     2461 gcgatatcgg cctgttcaag attgtgt
//
LOCUS       DATCXU010000190         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_375285,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000190 DATCXU010000000
VERSION     DATCXU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375285"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="sucD"
                     /locus_tag="VIR04_04220"
     CDS             complement(<1..810)
                     /gene="sucD"
                     /locus_tag="VIR04_04220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY9459985.1"
                     /translation="MSILINKDTKVITQGITGKTGQYHTHACREYANGKAAFVAGVHP
                     KKAGQNFEGIPIFGSVKDAKAQTGATVSVIYVPPAGAAAAIWEAVDADLDLVICITEG
                     IPVRDMLDVRNRMRDQNKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGIVSRSG
                     TLTYEAVAQVTELGLGQSSAVGTGGDPINGLKHIDILKMFNDDPETDAVIMIGEIGGP
                     DEVDAARWAKDNMKKPVVGFIAGVTAPAGKRMGHAGALISGGADTAGAKIEI"
     gene            complement(831..1991)
                     /gene="sucC"
                     /locus_tag="VIR04_04225"
     CDS             complement(831..1991)
                     /gene="sucC"
                     /locus_tag="VIR04_04225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY9459986.1"
                     /translation="MKIHEYQGKELLRKFGVTVPRGIPALSVDEAVAAAKKLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSLEEVRQLSSEILGMQLVTHQTGPQGQKVRRLLIEEGA
                     DIKKEYYVGIVTDRATQRIGVMASSEGGMEIEQVAESTPEKILKVFVDPEVGLTDAEA
                     AGLARGIGVPEASVSKAAGEFQKLYKTYCETDASLAEINPLILSGSGDIIALDAKFNF
                     DDNALFRHPDILAYRDLDEEDPAEIAASKFGLAYIQLEGNIACLVNGAGLAMATMDAI
                     KLFGGQPANFLDVGGGATAEKVTEAFKIMLKNDGVKAILVNIFGGIMRCDVIAEGIIA
                     ACKAVNLSVPLVVRMKGTNEKIGKKMLAESGLPIISADTMAEAATRAVAAAK"
     gene            complement(2043..2594)
                     /locus_tag="VIR04_04230"
     CDS             complement(2043..2594)
                     /locus_tag="VIR04_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEY9459987.1"
                     /translation="MPLPPPDTARKPLHTRSIRVSSFARDDGLWDLEAELIDVKAYDF
                     QKNNGQKHRAGDPLHHMHLRVTIDEQFTITSAVAAYDAAPYNEFCAAIAPDYRDLVGM
                     NLLRRFHQTVKERFGRTAGCTHMTELAFVLPTVAVQTMANKRRLEREQGQVKRPFQLD
                     GCHALRTDGPIAKEFYPRWYRAS"
     gene            complement(2730..>4877)
                     /gene="metE"
                     /locus_tag="VIR04_04235"
     CDS             complement(2730..>4877)
                     /gene="metE"
                     /locus_tag="VIR04_04235"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY9459988.1"
                     /translation="AKAGVKFVPVGDFAWYDHILEWSTLLGAVPARFAQSDSQPVSLD
                     TLFRMARGRAPSGTPAAACEMTKWFDTNYHYIVPELAPRQNYRIAREYLFDQIKEAQT
                     LGYQVKPVIPGPLTWLWLGKADVFAAGADDAAKLALLENLLPVYVEVLTRCKAMGVEW
                     IQIDEPILALDLPAQWKTAYAQVYSALTGLGINVLLATYFEGLKENVSVFKDLSVQGI
                     HIDLVRAPHQLDDIVAVLRDDQVLSAGVISGRNIWRTDLDQAKAVLQPVKAKLGDRLW
                     LAPSCSLLHVPVDLSVESELDPELCSWLSFATQKLDELHWLAASLDGTADADVTQALS
                     DQRKALADRRQSPRIHNAAVLKRLKESAGVSRVRAPFEQRIASQQKRLGLPAYPTTTI
                     GSFPQTREIRALRRDWKSGALSDAGYEKAIRAEIESVIRFQEKVGLDVLVHGEPERND
                     MVEYFGELLAGFAFTQNGWVQSYGSRCVKPPIIFGDVSRPAPMTVAWAAYAQSLTKKP
                     VKGMLTGPVTILQWSFVRDDQARETTCRQLALALRDEVVDLEKAGISIIQIDEPAFRE
                     GLPLRRSDWAKYLEWAVDCFRLSTSGVKEDTQIHTHMCYSEFNDIIESIAAMDADVIT
                     IETSRSNMELLGAFEDFRYPNDIGPGVYDIHSPNVPEVGWMVDLMKKAAGRLPRERLW
                     VNPDCGLKTRAWPETEAALVAMVKAAETLRQAV"
BASE COUNT         1109 a         1450 c         1248 g         1070 t
ORIGIN      
        1 gatctcgatt ttggcgccag cggtgtcagc accgccggaa atcagcgcgc cggcatggcc
       61 catgcgtttt cccgcagggg cggttacgcc cgcaataaat ccgacgacag gctttttcat
      121 attgtcttta gcccaccgag ccgcatccac ctcgtcggga ccgccaattt caccaatcat
      181 gattacggcg tccgtttcag gatcatcgtt gaacattttc aaaatatcga tgtgtttgag
      241 cccgttgata ggatcaccgc ccgtgccgac tgcgctcgac tgacccagcc cgagctcagt
      301 aacttgcgcg accgcttcat aggttagcgt gcccgaacgg cttacgatgc caatgcgtcc
      361 tttgcggtgt atatggcccg gcatgattcc gatcttgatt tcgtcagggg taatcagacc
      421 cgggcagttg ggcccaagca acaaggtttt cttgttttgg tcgcgcatgc ggttgcgtac
      481 atcgagcatg tcgcgcacag gaatcccttc ggtgatgcaa atgaccagat ccagatcggc
      541 atcgacagcc tcccagatgg cggccgctgc gcccgctgga ggaacgtaaa tgactgaaac
      601 cgttgcgccg gtttgggcct tggcgtcttt gactgaacca aaaataggta taccttcaaa
      661 attctgtccg gccttcttgg gatgcacgcc cgccacaaac gcagccttgc cgtttgcata
      721 ttcgcggcag gcatgggtgt gatactggcc cgttttgccg gtaattccct gggtaatgac
      781 ttttgtgtct ttattgatca aaatcgacat ttaaaaatcc ttcaatattc ttatttcgca
      841 gcagcgacag cccgggttgc cgcttcggcc atagtgtcag cgctaataat gggcaagccc
      901 gattcagcca gcatcttttt gccaattttt tcgttggtgc ccttcatgcg caccaccagc
      961 ggcacgctaa ggttcactgc tttgcaagcg gcaatgatgc cctcggcgat aacatcgcaa
     1021 cgcatgatgc cgccgaaaat gttcaccaat atggccttga ctccgtcgtt cttgagcatg
     1081 atcttgaatg cttcagtcac tttctctgca gtggcgccac cgccgacgtc cagaaaattg
     1141 gccggctgac cgccaaacaa cttgatcgcg tccatggtgg ccatcgccag accagcgccg
     1201 ttgaccaggc aagctatgtt tccctcgagt tggatgtaag ccagcccaaa cttgctggcg
     1261 gcaatctcgg caggatcttc ttcgtccagg tcgcgatacg caaggatgtc gggatgacgg
     1321 aacaaggcgt tgtcgtcgaa attgaacttg gcgtcgaggg cgataatgtc gcccgagccg
     1381 ctcagaatca gcgggtttat ttcggccagc gaagcatccg tttcgcaata ggtcttgtaa
     1441 agcttctgaa attcgcccgc ggccttcgac actgaagcct cgggcacccc aataccgcgc
     1501 gccaagccag ccgcttcggc atcggtcagg ccgacctcgg ggtcaacaaa taccttcaga
     1561 attttttcag gtgtggactc ggcgacctgt tcgatttcca tcccgccttc gctggaagcc
     1621 ataacgccaa tccgttgggt ggcgcgatca gtcacgattc cgacgtaata ttctttttta
     1681 atgtcggcgc cctcttcaat cagcaagcgg cgcacttttt ggccctgcgg accggtctga
     1741 tgcgtgacca gttgcatacc caagatttcc gacgacaact ggcgaacttc ttcaagagac
     1801 cgggccagct tgactccgcc ccctttgccg cggccacccg cgtggatttg cgccttgacc
     1861 acccaaaccg ggcctcccag tttcttggcg gccgccacgg cttcatcaac ggataaagcg
     1921 ggaatcccgc gcggcactgt cacgccaaat ttccttagca gttccttgcc ttgatactcg
     1981 tgaattttca tttattgcct gtcagaacat aaaaagtaaa aagatagtcg ccttgatatc
     2041 gatcaggaag cccgatacca ccgaggatag aactctttgg cgatcggccc gtccgtcctt
     2101 agcgcatggc aaccatcgag ttgaaacggc cgctttacct gcccctgctc tcgttcaaga
     2161 cggcgcttgt tcgccatggt ttgaacagcc acggtaggca atacaaacgc cagttcagtc
     2221 atatgcgtgc agcccgccgt gcggccgaaa cgctctttga ccgtctgatg aaaccgacgc
     2281 aataagttca tgcccaccag atcgcgatag tcaggggcaa tagccgcaca gaattcgttg
     2341 tagggcgccg cgtcataagc ggcaacagca gacgtaatag tgaactgttc gtcgatggtg
     2401 acgcgcagat gcatatgatg caaagggtcg cccgcccgat gtttctggcc attatttttc
     2461 tgaaaatcgt aagccttgac gtcgatcagc tcggcctcca gatcccacaa tccatcgtcc
     2521 cgggcaaatg aactgacacg tattgaacgc gtatgcagcg gcttgcgggc cgtatcagga
     2581 ggtggcaaag gcatggatca aaaacagaaa taaaatggta aaccgtgcaa gtataacgca
     2641 gcgctatcgg ccggacccgt ccaagcgctg tccaaaacaa aacacgcgga cgaaccgcgt
     2701 gttttgtttg ccggcaaacc agaacgtggt tacacagcct gacgcagcgt ctcggccgcc
     2761 ttcaccattg cgaccaaagc cgcttcagtc tcgggccatg cgcgggtttt cagaccacaa
     2821 tccggattga cccaaagacg ttcacgcggt agacgccccg ccgccttttt catcaaatca
     2881 accatccagc caacttcggg cacattgggc gagtgaatat cgtagacgcc cgggccgata
     2941 tcgttgggat agcgaaagtc ttcgaaggct ccgagcaatt ccatatttga acgcgaagtc
     3001 tcgatagtta ttacgtcggc gtccatcgcg gcgatcgatt cgataatgtc attgaactcg
     3061 gaatagcaca tatgcgtgtg tatttgagtg tcttccttga cgccggaagt cgacaggcgg
     3121 aagcaatcga cagcccattc cagatacttg gcccagtcgc tgcggcgtaa cggcaaacct
     3181 tcgcggaaag ccggctcatc aatttgaata atgcttattc cggccttttc caggtcaacg
     3241 acctcgtcgc gcaaagccag cgccaattga cggcacgttg tttcacgcgc ctgatcatcg
     3301 cgaacaaacg accactgcaa aatggtcacc gggccggtca acattccttt cacgggcttt
     3361 ttagtcagcg attgcgcgta ggccgcccac gccaccgtca tgggcgctgg ccgggacaca
     3421 tcgccaaaaa ttatcggcgg tttgacgcaa cgcgaaccat aactttgcac ccagccattt
     3481 tgcgtgaatg caaacccggc cagcaattcg ccaaaatatt ccaccatatc gttgcgctcg
     3541 ggttcaccgt gcaccagaac atcgaggccg actttttcct ggaaacggat gaccgattct
     3601 atttctgcgc gaattgcctt ttcgtatccg gcatccgaca aggcgcccga cttccagtcc
     3661 cggcgcaaag cccgtatttc acgcgtctga ggaaaagatc caatcgtggt ggtgggataa
     3721 gccggcaagc ccaggcgttt ctgctgactg gctatgcgct gctcgaacgg cgcacgaaca
     3781 cgcgatacgc cggccgattc tttcaaacgc ttcaataccg ccgcattatg gatgcgcggc
     3841 gattgacgac gatcagcaag cgccttgcgt tgatcggaca aagcctgcgt gacgtccgcg
     3901 tcggcggtcc cgtccaaaga cgccgccagc cagtgcaact cgtcgagctt ttgcgtggca
     3961 aacgacaacc aactacacaa ttccggatcc agctcggatt caacgctcag atcgaccggc
     4021 acatgcaaca acgagcagga gggcgccagc cacagccgat ccccgagttt cgccttgaca
     4081 ggctgcaata ctgccttggc ctggtccaga tcggtgcgcc aaatattccg gccactgatc
     4141 acgccggctg aaagcacctg gtcatcgcgc aagacggcaa caatgtcatc caactgatgc
     4201 ggagcgcgca ccagatcaat atgaatgcct tgcacagaca gatctttaaa gactgaaacg
     4261 ttttccttca agccttcaaa atacgtcgca agcagaacat tgatgcccaa gccggtcaaa
     4321 gccgaataaa cttgggcata tgccgttttc cactgtgcgg gtagatcaag cgcgaggata
     4381 ggctcatcta tctggatcca ttccaccccc atggctttgc agcgcgtcag cacttcgacg
     4441 tacacaggca acagattctc gagcagggcc agcttggccg catcgtcggc gccagcggca
     4501 aacacgtccg ccttgcccag ccacaaccag gtcaaaggac ctggaataac gggcttgacc
     4561 tgataaccca atgtttgcgc ttctttaatt tgatcaaaca agtactcgcg cgcaatgcga
     4621 taattttgcc gcggcgccag ttccggcaca atatagtgat agttcgtgtc gaaccacttg
     4681 gtcatctcgc acgctgcagc cggcgtcccg gaaggggcgc ggccacgcgc catgcgaaac
     4741 aatgtatcca gactgaccgg ctgggaatcc gattgcgcga atcgcgctgg cacggcgccc
     4801 agcagcgtgc tccactccag aatatggtca taccaggcaa aatcgcccac gggcacaaac
     4861 tttacccctg ccttggc
//
LOCUS       DATCXU010000191         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_387114,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000191 DATCXU010000000
VERSION     DATCXU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387114"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /gene="nuoL"
                     /locus_tag="VIR04_04240"
     CDS             complement(<1..1916)
                     /gene="nuoL"
                     /locus_tag="VIR04_04240"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY9459989.1"
                     /translation="MNSLGLYLVIALAPLAGALLIGFFGTGFLGRQVSRSLSHSVAIL
                     GVLISFIGSLIVLFQVLDGHTFNGVVYTWTAIGSVKLDVGFLIDPLSALMMVTVSFVS
                     LMVHIFTIGYMRDDPGYSRFFSYISLFTFSMLMLVMSNNMLQLFFGWEAVGLVSYLLI
                     GFWYTRPKAIFASLKAFLVNRVGDFGFIVGIGLLFAYAGSMNYAEIFPQADKLGAIVF
                     PGTNWQMLTVACVSLFVGAMGKSAQVPLHTWLPDSMEGPTPISALIHAATMVTAGIFM
                     VARFSPVFEQSATALSVMIIIGAIGALFMGLVGIVQNDIKRVIAYSTLSQLGYMTAAL
                     GASAYSAGIFHLMTHAFFKALLFLAAGSVIMAMHHDQDIRNMGGLRKYMPVTALTFLL
                     GTLALVGTPFFAGFYSKEHVIEAIGAANVWGAGFAYYATVIGVFVTSLYSFRVYFLVF
                     HGKEKFDAPPFPYQPPVVNDDYYAHDNHAHGEEHGHEGPPKESPWVVTGPLVLLAIMS
                     VIAGAWFVDPLLFGNFFHSSIAILPTHPAMAELAEHWHGWVAYGLHGFVTTQFWLMMA
                     GLVVAWYCYLINPAVPAAIQRKFSGLYSVLINKYYADWFNEQVVARGARALGHGLWHG
                     GDRIVIDGAVNGSWR"
     gene            complement(1933..2241)
                     /gene="nuoK"
                     /locus_tag="VIR04_04245"
     CDS             complement(1933..2241)
                     /gene="nuoK"
                     /locus_tag="VIR04_04245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY9459990.1"
                     /translation="MTITLAHYLILGAILFAIGVFGFFQNRRNLIILLMSIELILLAA
                     NMNFVAFSSWSGENMGQVFVFFILTVAAAEAAIGLAILVLLFRNLNTINVDELDQLKG
                     "
     gene            complement(2238..2846)
                     /locus_tag="VIR04_04250"
     CDS             complement(2238..2846)
                     /locus_tag="VIR04_04250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY9459991.1"
                     /translation="MVLVVAAFRVVNATNSVTAVLHLILAFFTASMLWILLGAEFLAL
                     MLVLVYVGAVMVLFLFVVMMLDIRVDMLREGLKTYLPLGLTVGVVMVLEMSFVLAKTW
                     LGAGEQAPVANNYDNTHALGMAMYTNYVFAVEVGAVILLVGMIAAIALTLRQRHDVKY
                     NNPADQVRVRARDRVRLVSLPSQTEAPEPAPAATGDEVGKTK"
BASE COUNT          783 a          859 c          721 g          488 t
ORIGIN      
        1 cgccaactgc cgttaacggc gccatcaatc actatacgat cgccgccatg ccagagcccg
       61 tggcccaatg cacgagcgcc gcgcgctaca acttgctcgt tgaaccagtc ggcgtagtac
      121 ttgttgatca gcaccgaata aagtccggag aacttgcgtt gaatggccgc gggcacagcc
      181 gggttgatca aatagcaata ccacgccacc accaacccgg ccatcatcag ccagaattgc
      241 gtcgtgacaa agccgtgcag accgtaggct acccaaccat gccaatgctc ggccagctcg
      301 gccattgccg gatgggtcgg cagaatggcg atcgaactat ggaaaaaatt accaaacagc
      361 aaaggatcca caaaccaggc gccggcgata acggacatga ttgccagcaa taccaacggt
      421 ccggtaacca cccacggcga ctccttcggc ggcccttcat gcccgtgctc ctcgccgtgc
      481 gcgtgattgt cgtgcgcata ataatcgtcg ttcaccacag gcggctgata cggaaacggg
      541 ggtgcatcga acttctcttt gccgtgaaac accaggaagt aaacacggaa tgaatacagg
      601 gacgtgacga agacgcctat tacggtcgcg tagtacgcaa accccgcccc ccacacattg
      661 gccgcgccaa tggcttcgat tacgtgctcc ttggagtaga agcccgcaaa aaacggagtg
      721 ccgaccagcg ccagggtgcc cagcaagaac gtcagggcag taaccggcat gtatttgcgc
      781 agtcctccca tgttgcggat atcctgatca tggtgcatgg ccataatcac cgaaccggcc
      841 gcaagaaaca gcaaggcttt gaagaaggca tgcgtcatca gatggaagat gcccgccgaa
      901 taggccgacg cgcccagcgc cgccgtcatg tagcccaatt gcgacaatgt tgaataagcg
      961 ataacgcgct tgatgtcgtt ttgaacaatg cccaccagcc ccatgaacag cgcaccgatg
     1021 gcgccgataa tgatcattac cgacaatgca gtggcggact gctcaaagac cggcgaaaag
     1081 cgcgccacca tgaatatgcc ggccgtaacc atcgtggcgg catggatcaa cgccgagatt
     1141 ggcgtcgggc cttccatgga atcgggcagc caagtatgca aaggcacttg cgccgatttg
     1201 cccatggctc ccacaaacaa ggaaacgcac gccacagtca gcatctgcca gttcgtgccc
     1261 ggaaaaacga tagcgcccag cttatcggcc tgcggaaaga tctcggcata attcatgctg
     1321 cctgcatagg caaacaacag gccgattcca acgataaaac cgaagtcgcc cacacggttg
     1381 accaaaaacg ccttcaggct ggcaaaaatc gcctttggcc gtgtgtacca gaagccgatc
     1441 agcaagtatg aaaccagacc caccgcttcc cagccgaaaa acagttgcag catgttgttg
     1501 gacatgacca gcatcagcat ggaaaacgta aagagcgata tataagaaaa gaaacgcgaa
     1561 tagcccgggt catcgcgcat atagccaatc gtgaagatat gcaccatcag tgacacaaag
     1621 gaaaccgtaa ccatcatcaa cgccgacaat gggtcgatta aaaatccaac gtccagcttg
     1681 actgagccga tcgcggtcca ggtgtaaacc accccgttaa acgtgtgacc gtccagcacc
     1741 tgaaaaagca cgatcaacga tccgatgaat gaaatcaaga cgccgagaat ggcgacagaa
     1801 tgagaaaggc ttcgactgac ctgccgtccc aaaaagccag tgccgaaaaa tccgatcagc
     1861 agcgccccgg ccagcggagc aagcgcgata acaagatata gaccaagtga attcatattc
     1921 tgtgctatcc catcaacctt taagctggtc aagttcgtca acattgatcg tattcagatt
     1981 gcggaacagc aacaccagaa tggccagacc aatcgccgcc tcggccgccg ccacggtcaa
     2041 aatgaaaaat acgaacacct gacccatgtt ttcgccggac cagctcgaaa aggccacgaa
     2101 attcatgttc gcggccagca atatcagttc aatcgacatc aacaaaataa tcaggttgcg
     2161 ccggttctgg aaaaaaccga acaccccaat cgcgaacagt atcgcgccca atatcagata
     2221 atgcgccaag gtaatagtca ttttgtcttt cccacttcgt cgccggtcgc agcgggcgca
     2281 ggctcgggcg cttccgtttg cgatggcagg ctgaccagcc gcacacgatc gcgggcgcgt
     2341 acgcgcacct ggtcggccgg attgttgtat ttcacgtcat ggcgttgacg cagagtcagc
     2401 gcgatggcag ctatcatgcc caccagcagt attactgcgc ccacttcaac agcaaataca
     2461 taattggtgt acatcgccat gcccagcgca tgggtgttgt cgtaattatt ggcgaccggc
     2521 gcctgttcgc ccgcgcccag ccaggttttg gccagcacaa acgacatctc cagcaccatc
     2581 accacgccca cggtcaggcc caggggcaga taggttttca acccctcgcg cagcatgtcc
     2641 acgcggatgt cgagcatcat caccacgaac aggaacagca ccatgaccgc ccccacataa
     2701 accaggacca gcataagcgc caggaattcg gccccaagca ggatccacag catggacgcg
     2761 gtaaaaaagg cgaggataag gtgcaggacg gctgttaccg aattggtcgc gttgaccacg
     2821 cgaaacgccg ccaccaccag caccagcgcc a
//
LOCUS       DATCXU010000192         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_241527,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000192 DATCXU010000000
VERSION     DATCXU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241527"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VIR04_04255"
     CDS             <1..879
                     /locus_tag="VIR04_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9459992.1"
                     /translation="AAAAKLKGLNHEQVVRALAIAGSQSAGLRENFGTMMKPFHAGRS
                     SESGVVAAEFAALGWTGTDKILEAGRGFFRAAGGGFDPQSIVGKLGKPWTLVSPGVSI
                     KPFPSGSLTHPGMTELLELILEHDIKPEDVVSLDVGTNQNMPNALIHHHPRNELQAKF
                     SMEFCMAILLLERRAGLPEFTDEVVNRADVKALIEKVNFHVHPEAEAAGYDKMTTILD
                     IHLRDGRRIRGRSDFGKGSPANPMSYDEVADKFRGCARFAKWPQERAERIIELVRKLE
                     SVGDIRELTSLCGKTA"
     gene            complement(898..1752)
                     /locus_tag="VIR04_04260"
     CDS             complement(898..1752)
                     /locus_tag="VIR04_04260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459993.1"
                     /translation="MKKIRPPKFPTYVSTGERLWHYLHRLICILTLIFLLLPIFAIVP
                     LSFSDSTLLLYPIPSFSFKWYDALFHSDEWIRAAKNSFIVAPSATLIATVLGTLAAAG
                     LHRIEFHGKGLLMAILISPMIVPLVVVGVGIYLFFAPYGLTSSYIGLILAHAALGAPF
                     VLTTVAATLQGFNENLVRASYSLGASPLHTFFSVTLPIIAPGVIAGALFAFATSFDEI
                     VVTLFLAGPEQTTLPRQMFTGIRENINPIIAAVATILIIFSTTLLLTLEWLRSRAQAK
                     TILSDKTP"
     gene            complement(1754..3010)
                     /locus_tag="VIR04_04265"
     CDS             complement(1754..3010)
                     /locus_tag="VIR04_04265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9459994.1"
                     /translation="MIATRADNDAHTIRHAMRAAERRRKWRAFFLVAPLGLFLLVIFV
                     IPIGALFQRAVENPEVISTLPNTLRTLKNWDGSTVPSDASYAALATDLSRTKGSSAAG
                     GLARRLNYEIPGYRSLIFKTIRRMPLTSPGDKALNDADIRARLIDIDRHWAEIPFWQA
                     IANNKTEYSPYYLLASLDLKQNAQGDIIRAPAGTSAFLDIFGRTFLISGIVTLLTLIL
                     GFPLAYWITTLPRHKANLIFICILIPFWTSVLVRIAAWIVLLQRQGLVNNALMNMGIT
                     DVPLALLFNRAGVYIAMTHILLPFMILPIYSVMQSVPNTYQRAAISLGSHPFSAFWRV
                     YVPQTYAGVGAGTLLVFIIAIGYYITPALLGGADDQMISYYVAYFTNQTINWGMASAL
                     GFILLIGTLLLYSLYRKLSGRELGLG"
BASE COUNT          722 a          790 c          961 g          627 t
ORIGIN      
        1 gcggccgccg ccaagctgaa gggcctgaat catgaacagg tggttcgcgc tttggccata
       61 gccggcagcc agtcggcggg cctgcgcgaa aatttcggca ccatgatgaa acctttccat
      121 gccgggcgat catcggaaag cggcgtggtc gcggctgagt tcgctgcatt gggctggacc
      181 ggcaccgaca agatactgga agccggacgc ggctttttcc gcgcggcggg cggcggtttc
      241 gacccacaat ccatcgtggg taaattgggc aagccttgga ctcttgtatc gcccggcgta
      301 tccataaagc ccttcccatc cggctcgctg actcatccgg gcatgacgga gcttcttgag
      361 ctaattcttg agcacgacat caaaccggag gacgtcgtga gcctggatgt cggcacgaat
      421 cagaacatgc ctaatgcgct catacaccat catccccgta acgagctgca ggcgaaattt
      481 tcgatggaat tttgcatggc gattctattg ctggaacggc gtgcaggctt gcccgaattc
      541 actgatgagg ttgtgaatcg ggccgatgta aaggcgctga tcgagaaagt gaattttcat
      601 gtgcaccccg aagccgaagc ggccggttac gacaagatga cgacgatcct ggatatccat
      661 ttgcgtgacg ggcggcgtat tcgcgggcgc tctgattttg gcaagggaag cccggccaat
      721 cccatgagct atgacgaagt ggcggacaag ttccgtggct gtgcgcgttt tgcaaaatgg
      781 ccgcaagagc gggccgaacg tattattgag cttgtgcgca aacttgagtc ggttggcgac
      841 atcagggaac tgacgtccct gtgcggcaag acagcttagt gctggacgat cttttggcta
      901 tggtgttttg tcgctgagta tcgtcttggc ttgtgcgcgc gagcgcagcc attccagggt
      961 gagcagcagc gtagtcgaga agatgatcag tatggtggcc acggcggcga tgatgggatt
     1021 gatgttttcg cgtatgccgg taaacatctg gcgcggcagc gtagtttgct cggggccagc
     1081 caaaaacagc gtcaccacga tttcgtcaaa ggatgtggcg aacgcgaaca acgcaccggc
     1141 tatcacgccg ggcgcgataa tgggcagggt aacgctgaaa aacgtgtgca gcggactggc
     1201 gcccagactg taactggccc gcaccaagtt ctcattaaac ccttgcagtg tggccgcgac
     1261 ggtggtcagt acaaagggcg cacccaaggc cgcgtgcgcc agaatcagac cgatataact
     1321 gctcgtgagg ccataaggcg caaaaaataa atagatccct acaccgacca ccaccagcgg
     1381 cacgatcatt ggcgagatca gaattgccat cagcaaacct ttgccgtgaa attcgatgcg
     1441 gtgcaatccc gcggcggcga gcgtgcccag cacggtggcg ataagtgtgg ccgatggcgc
     1501 aacaataaaa ctgttttttg cggcgcgtat ccattcgtcg gaatgaaaaa gcgcgtcgta
     1561 ccacttgaac gaaaaagaag gtatgggata gagcagtagc gtgctgtcgc taaaggacag
     1621 cggcacgatg gcgaaaatgg gaagcaaaag aaaaatcagc gtcagtatgc aaatcagacg
     1681 gtgaaggtaa tgccacagac gttcgccggt agatacgtag gtcggaaatt ttggcgggcg
     1741 aattttcttc atttcaaccc aatcccaatt cccggccgga caatttgcgg tacagggaat
     1801 acagcaacag tgtgccaatc agtaaaatga aacccagtgc gctggccatg ccccaattga
     1861 tggtctggtt ggtgaaatag gccacgtaat agctgatcat ctggtcgtcg gcgccgccca
     1921 ggagggctgg cgtgatgtaa tagccgattg cgatgatgaa gaccaggagc gttcccgcgc
     1981 cgacgccggc ataagtttgc ggaacgtata cacgccagaa tgcggagaaa ggatggctgc
     2041 cgagggaaat ggcggcgcgc tgatacgtat taggcaccga ctgcatcacg ctgtaaatgg
     2101 gcaaaatcat gaaaggcaat agtatgtgcg tcatggcgat gtagacgccc gcgcgattaa
     2161 acagcaaggc gagcggaacg tcggtgatgc ccatgttcat tagggcattg ttgaccaggc
     2221 cctgacgctg cagcaatacg atccaggcag cgatgcgcac gagcaccgaa gtccagaatg
     2281 gaatcagtat gcagatgaag atcagattgg ccttgtgccg gggcagcgtg gtgatccagt
     2341 atgccagcgg gaaacccaga attagcgtca gcagtgtgac aatgccgctg ataagaaacg
     2401 tgcggccaaa tatatccagg aatgccgacg tgcctgcggg agctctgatg atgtcgccct
     2461 gagcgttttg tttcaggtca agcgaggcaa gcagatagta gggagaatac tccgtcttgt
     2521 tgttggcaat ggcctgccag aatggaattt ccgcccagtg cctgtcgatg tcgatcaggc
     2581 gggcgcggat gtccgcatcg tttagcgctt tatcgccggg cgatgttaaa ggcatccggc
     2641 gtatggtttt gaagattagt gaacgatagc cagggatctc atagttcagt cgccgcgcca
     2701 ggccgccggc ggcgcttgag cctttagtcc ggctcaggtc cgtagccagc gcggcatacg
     2761 aggcatcaga tggaactgtc gaaccgtccc agtttttcaa tgtccgcaga gtattgggca
     2821 gtgtggagat gacttcgggg ttttcaaccg cgcgttgaaa gagcgcgccg atgggaataa
     2881 cgaaaatgac aagcaaaaac agtcccagcg gggccaccag aaagaatgcg cgccacttgc
     2941 gccggcgctc ggccgcccgc atcgcgtgcc tgattgtgtg cgcgtcgttg tcggctctcg
     3001 ttgcgatcat ggtttggcct gtgggcggcg cccccgcgtc ggcttgcaag ctgcgaaggg
     3061 cgccaggtgc aatcggctac cggctttatc gggcggccca
//
LOCUS       DATCXU010000193         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_168189,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000193 DATCXU010000000
VERSION     DATCXU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168189"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..764)
                     /locus_tag="VIR04_04270"
     CDS             complement(90..764)
                     /locus_tag="VIR04_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY9459995.1"
                     /translation="MRVLVIEDDTTLGHALQEFLIGQGYAVDWLQDGGQVAGAVAGQT
                     YDLLLLDLNLPGLSGLDVLRQLRGNGQQVPVLILTARDGVEDRVAGLDAGADDYVTKP
                     FELPELAARVRAFARRRAGQAQPFIEAGPLLFDTVGREVRVNGERLSLSVRELSVLEM
                     LMGRAGRVVTKRQIVNSLSAWDADFSENAVEVYVYRLRKRLERTGASIQTVRGFGYLL
                     DVESAA"
     gene            complement(828..1265)
                     /locus_tag="VIR04_04275"
     CDS             complement(828..1265)
                     /locus_tag="VIR04_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY9459996.1"
                     /translation="MAFGSFDSKAESHTLSEINMVPLIDVMMVLLVIFMITAPLLSHS
                     IKINVPQANSQPVENQPKSIDLAVDAQGQLFWNEQAIASDQLPSRLAQQARLDPQPEL
                     RIRADLDTRYQILAEIMSRAKQAGIRRMGFITRPDADLKAPHL"
     gene            complement(1268..2065)
                     /locus_tag="VIR04_04280"
     CDS             complement(1268..2065)
                     /locus_tag="VIR04_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY9459997.1"
                     /translation="MFDLIHSALQVLSQIGGAAPAAAAQDVGISQFVSQSDWIGKSLF
                     GFLILMSIATWYLIVVKAFLNFRTRRRSAQFLREFWAAVSLEQVADQIKRHGASEPFS
                     RVANNAIYAHNRQVSRGVKPDAKGPADAFVARTIRKIIDEETAALENGLTVLASVGST
                     APFVGLFGTVWGVYHALIGISLSDGVTINRIAGPVGEALIMTGLGLAVAIPAVLAYNG
                     FVRGNRVYLARLDAFAHDLFTFLLTGQQVGDPDLEMQAEPLAAAKED"
     gene            complement(2075..>2358)
                     /locus_tag="VIR04_04285"
     CDS             complement(2075..>2358)
                     /locus_tag="VIR04_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY9459998.1"
                     /translation="QPRLIGKVDYLGSRPHPDYPRVSMRRGETGRVVVRVFISRQGLV
                     EKATVRTSSGFSRLDESALNAVRAARFKPYTENGVPYPAIADIPFDFVL"
BASE COUNT          553 a          722 c          624 g          459 t
ORIGIN      
        1 gcagcgacgc caccagatgg cgggccaacg accccttggg gatcagagtg ccgaaccgcc
       61 gtcgaataag tctattgacc tggaacgcgt tacgccgcac tttccacgtc aagcaaatag
      121 ccgaaacccc ggactgtttg aatgctggcg cccgtgcgtt ccagccgctt acgcaaacgg
      181 tatacgtaga cctccaccgc attctcgctg aaatcggcgt cccaggctga taaagagttc
      241 acgatttggc gtttcgttac aacgcgtcca gcccggccca tgagcatttc aaggaccgaa
      301 agttcgcgaa ctgacaggga cagccgttcg ccgtttacgc ggacctcgcg ccccactgtg
      361 tcaaatagca aggggcccgc ttcgataaac ggctgggcct ggccagcccg gcggcgggcg
      421 aaagcgcgca cgcgagccgc gagctcgggc aactcgaaag gcttggtgac ataatcatcg
      481 gccccggcgt cgagccccgc aacgcggtcc tcgacaccat cgcgggccgt caaaataagt
      541 accgggacct gctgtccgtt gccgcggagc tggcgcaaaa cgtcaagacc gcttaaaccc
      601 ggtaaattca ggtcaagcaa taaaagatcg taggtctggc ccgcgaccgc gcccgccact
      661 tggccgccat cttgcagcca gtcgacggca tagccctgcc cgataaggaa ctcttgcagt
      721 gcatggccca acgttgtgtc atcttcgatt actagaactc gcatggcatg attctatatc
      781 gaacagccgg aatttgcccc attttgggct aattttggct ggtggcgtta caaatgcggc
      841 gccttcaggt cggcgtcagg ccgggtaata aaccccattc gcctgatgcc ggcctgtttg
      901 gcgcgactca taatctcggc caggatctgg tagcgcgtat cgaggtccgc gcgaatgcgc
      961 agctcgggct gcggatccag ccttgcctgc tgcgctagcc gcgacggtaa ctggtcgctt
     1021 gctatcgcct gctcgttcca gaatagttga ccttgcgcat caacagccag atcgatggat
     1081 ttaggctgat tttcgacagg ctgtgaattg gcttgcggaa cattaatctt gatcgaatga
     1141 cttaacagcg gcgcggtaat catgaatatc accagaagca ccatcatgac gtcgatcagc
     1201 ggcaccatat taatctctga aagcgtatga ctttcggctt tactatcgaa acttccaaac
     1261 gccatgatca atcctccttc gcagccgcca gcggctcggc ttgcatttcc agatcagggt
     1321 cgcccacctg ctgccccgtc aataaaaagg tgaacaggtc gtgcgcgaac gcatcgagcc
     1381 gcgcgagata aacgcggttg ccgcgtacga agccgttata agccaggacg gcagggatcg
     1441 cgacagccag ccccaggccc gtcatgatca aggcttcgcc aaccgggccg gcaatccggt
     1501 tgatcgtcac accatccgac aggctaatcc cgatcagagc gtggtatacg ccccaaaccg
     1561 tgccaaatag cccaacaaaa ggcgccgtcg aacccaccga tgccaaaaca gtcagaccgt
     1621 tttccagcgc agcggtttct tcgtcaatga ttttgcggat cgttcgcgcg acaaacgcat
     1681 cagccgggcc tttcgcatcc ggtttcacac cgcggcttac ctggcgattg tgcgcgtaaa
     1741 tggcattgtt cgccacgcgg gaaaaaggtt cggatgcgcc atgccgcttg atctgatccg
     1801 caacctgctc aagagaaacc gccgcccaga actcgcgaag aaattgagcc gagcggcgac
     1861 gagttcgaaa attcaaaaat gcctttacca cgatcaggta ccacgtcgca atcgacatca
     1921 gaatcaggaa tccaaacaaa gattttccta tccaatcgct ttgtgaaaca aattgcgaaa
     1981 tcccgacgtc ctgcgccgcc gcagcgggcg ccgccccgcc gatctgggac aacacctgca
     2041 acgcactgtg aataagatcg aacatgggtg attcctatag aacaaaatca aatggaatat
     2101 cggctattgc cgggtacggc acgccatttt ctgtgtacgg tttaaaccgc gccgcccgca
     2161 ctgcgttcag ggcagactca tcaagtcgcg aaaaacccga agaagtgcgc acggttgcct
     2221 tttccaccaa tccctgacgg gaaataaaca cgcgcaccac cacccgtccc gtttcgccgc
     2281 gccgcattga aacgcgcgga tagtccggat gggggcggct acccaaataa tcgactttac
     2341 caatcaggcg cggctggt
//
LOCUS       DATCXU010000194         9002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_414059,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000194 DATCXU010000000
VERSION     DATCXU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414059"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VIR04_04290"
     CDS             <1..1023
                     /locus_tag="VIR04_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY9459999.1"
                     /translation="AKGLIGIVLPGGILFFWLLGRRRFDTMRRLFTWVGAGVFLLVSL
                     PWMLAMQRLYPGFFDYYIIYQHFERFLEKGFNNPYPFWFYIPVLLGLTLPWSLQLWRV
                     FNKTYWSDSAYGAVRGLMLSWLLVVLIFFSVPSSKLVGYVLPALAPFAFFLAEPFAAR
                     LRGAGAARALTTLTGFLLVSIVICVAAVTTLIVAPQPSSRALAGQMLTSYRPADKVVM
                     LNRYSYDLGFYLHTSKSVFVVSGWDNPDLKFQDNWRKELFDAGQFEPDTAARLLIKPQ
                     DLVDKLCADRVITLWLWGDKNSVADYPFLASISPYAADGKSRVWRIQAGPALSFCAER
                     PKSGQE"
     gene            complement(983..1360)
                     /locus_tag="VIR04_04295"
     CDS             complement(983..1360)
                     /locus_tag="VIR04_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY9460000.1"
                     /translation="MKQLCWFVAVGCAAAATHWLVVVAVVSFAGYAPLIANVIGWLIA
                     FCVSFLGHYQLTFKLQHAPLSRALRRFFAVSALGFGVNELAYAYLLHATTLRYDVLLA
                     LILIAIAVMTFILGRFWAFRRKN"
     gene            1396..2232
                     /locus_tag="VIR04_04300"
     CDS             1396..2232
                     /locus_tag="VIR04_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEY9460001.1"
                     /translation="MVKVKEKHIVICADDFGMDSGIDDGILRLAQLKRLNATSCLVEG
                     PSFRRNAAALNDSALQLGLHLNFTESMGEAGLYLPVSSLIVRSYLRRLDDAQVHAQIA
                     RQLDLFEASFGRAPDFVDGHQHVHQLPQIRQALLAELTRRYSPRSPAPEAGARACPWL
                     RHTRAGSQSGLPLRLRLKAWIIQSLGSRGLFQSASSAGFSMNAGLLGVYDFRGGVISY
                     QTLLKRWLEYAVDGDEIMCHPAARAVDGDVLGEQRVAEFNVLAGDRAGQWLCDYGIAL
                     RV"
     gene            complement(2245..2781)
                     /locus_tag="VIR04_04305"
     CDS             complement(2245..2781)
                     /locus_tag="VIR04_04305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY9460002.1"
                     /translation="MDSTQLQIRDATHDDMSAVQRIYASYVLNSTATFEEKPPDVAEM
                     QSRYEHVRMLGLPYLVGIANGVLIGYCYAAPYRPRIAHRYTIENSIYLAEDQTGKRFG
                     GALLEELIKRCERGPWRQMIAVIAGGDNVASIALHRRLGFRDAGTQPSTGYKFGQWID
                     VVFMQRALGAGDSTHPTE"
     gene            2836..3177
                     /locus_tag="VIR04_04310"
     CDS             2836..3177
                     /locus_tag="VIR04_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY9460003.1"
                     /translation="MSVSINALTDAFAVAPQLSLDDIADVAAAGYKSVIINRPDFEGG
                     PDQPTAADIMAAAKTAGMEAEYLPVVSGAATRQDAVRFAQLLKQLPAPVLAYCRTGTR
                     SAHLYHEANAL"
     gene            3276..3737
                     /locus_tag="VIR04_04315"
     CDS             3276..3737
                     /locus_tag="VIR04_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY9460004.1"
                     /translation="MFKRILIPTDGSALSAQAANKGVCFARQIGAEVVALYVVQPFAA
                     TVGFDGMAAAYAITDEDYDKTAAEQAQKYLKAVLDRAITAGVKATGRTVSNFNVADGL
                     VQAAEEEKCDLIFIGSHGRSGLSRLLLGSVTVKVLGLAKTAVLVYRVKESE"
     gene            3831..4931
                     /gene="moaA"
                     /locus_tag="VIR04_04320"
     CDS             3831..4931
                     /gene="moaA"
                     /locus_tag="VIR04_04320"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY9460005.1"
                     /translation="MKKVVFLPDRRFSAQPEAAPTFAPVPGQPVLDTRNRPLRDMRIS
                     VTDRCNFRCTYCMPREVFGSDYQFMPHASLLTFEEITRIVRASVALGVEKIRLTGGEP
                     LLRKNLETLVEMLARLQTPQGKPVDLTLTTNGTLLAKKAQALKDAGLARVTVSLDALD
                     ENLFARMSDSQVGVKTILRGIDEAARVGLAPVKINMVVQKGVNEDQLVPMARHFRHSG
                     HILRFIEFMDVGTTNGWNLAQVLTGAQILKIVGAEFPLDPVPAAYRGEVAVRWRYVDQ
                     AGEIGVITSVSQPFCADCTRIRLSPDGKLFLCLFATHGHDLRALVRGNASDEQIAARL
                     ATVWMGRDDHYSELRGKETLKEKKIEMSYIGG"
     gene            4969..5487
                     /locus_tag="VIR04_04325"
     CDS             4969..5487
                     /locus_tag="VIR04_04325"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY9460006.1"
                     /translation="MKFGWQKQMAAIGLAAVMWAGSLSVGMAQSAPAVGDTVTVPAVT
                     LIDGQRLPAGYFQGKPLIVEYWASWCPFCGRQNPRLQKLTLEAQKKGLQILTVSVDRK
                     ESDASDYIQEHHYTFPVAMETPELLKTFGRRDIIPEIFVVTANGKLAEVLSGELPEAQ
                     VAGLIKYAPGAH"
     gene            complement(5492..5950)
                     /locus_tag="VIR04_04330"
     CDS             complement(5492..5950)
                     /locus_tag="VIR04_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY9460007.1"
                     /translation="MVIVQEADFNEAELVAQLRERTAGKAGSIVTFTGYVRDYASNKT
                     TQSLYLDHYPGMCEREIESICATAQTRWNTLGAVVMHRVGELRYKEKIVFIAVACAHR
                     GDAFNACQYIIDALKTRAPFWKRETLASGERFWVQQHEADFVKATQWESD"
     gene            complement(5955..6206)
                     /locus_tag="VIR04_04335"
     CDS             complement(5955..6206)
                     /locus_tag="VIR04_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY9460008.1"
                     /translation="MSGAAIKVLYFAQVAELVQVRQESWPLMQAVTGAAWLRQLESRY
                     PQLAPARRLKLAVNQRHIAHDMLIAPGDEVAVFEPVTGG"
     gene            complement(6187..6684)
                     /gene="moaC"
                     /locus_tag="VIR04_04340"
     CDS             complement(6187..6684)
                     /gene="moaC"
                     /locus_tag="VIR04_04340"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY9460009.1"
                     /translation="MNPSESSELKLSHLDESGQIRMVDVSDKAPSQRVAIAFGRVRMS
                     PAAYALLTAAENSKGEVLNTARVAGVLAAKRCPELIPLCHSLPLSFAGIEFSLDEKSS
                     CITIKATCRSNYKTGVEMEAMTACSVAALTIYDMCKAADKGIILETIKLSYKSGGKSG
                     EWRCN"
     gene            6876..7379
                     /locus_tag="VIR04_04345"
     CDS             6876..7379
                     /locus_tag="VIR04_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY9460010.1"
                     /translation="MTIKVGERVPESTLAEFIETETEGCSLGPNNFQVSSLVKGKTIA
                     LFAVPGAFTPTCSNKHLPGFLEASDKLKAKGVDEIWCLAVNDPFVMGAWGRDRQVNGR
                     IRMMADGSAAWTKALGLELDLVGRGMGVRSQRYSAILKDGIVRHLNVELPGKFEVSDA
                     KTLLGQL"
     gene            7407..8681
                     /locus_tag="VIR04_04350"
     CDS             7407..8681
                     /locus_tag="VIR04_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9460011.1"
                     /translation="MLKAIAAFSSLYAATLLLLLGTGLFNTYLGLRLTASSVSEVWVG
                     AMIAVYYLGLVFGARFGHTLIVHVGHIRAYSASAAIATVSVLALALLNSLWVWLAFRF
                     IAGIAMVTQFMVIESWLNEQTENQVRGRVFAFYMVFSGLGTVLGQLSLTLFPRLDYQP
                     LIFVAICSALGLVPVVLTRRVHPALQKPAPIMIKYYMSRVPLSLVVLFMAGTIIGAFY
                     GLAPVYAAKHALSSDQVAIFLAVAVASGLMSQWPLGWLADRLNRAVLIRVNAGLLCML
                     GIPMWGWWEFPFWALVAFSCVFGILQFTLYPLGVAFANDNIDPERRVGLSAILYMVYG
                     LGACAGPFIVGVFMRQFSVDMFYVFVSFCAAVLVIFVRPERVTGANRSNDAPTQFVPM
                     SDSLQSSPVAAVLDPRVDAQSDISSDPAVSAR"
     gene            complement(8702..>9002)
                     /locus_tag="VIR04_04355"
     CDS             complement(8702..>9002)
                     /locus_tag="VIR04_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460012.1"
                     /translation="VLAGIVIFLVLVIQRTQWSLRNIVAGIVLGLLNFGNIYFYIRAH
                     QVFPHNPTLVFSAMNIGVISVGTLVGAGLFKEKLSWVNVLGIALAITAIIVLIPR"
BASE COUNT         1802 a         2362 c         2629 g         2209 t
ORIGIN      
        1 gccaaaggcc tgatcggcat tgtcctgcca ggcggcatat tgtttttctg gttgctggga
       61 cgtcgtcgct tcgacacgat gcggcgcctg ttcacgtggg taggcgcggg ggtgtttctg
      121 ctggtgtctt tgccatggat gctggcgatg caaaggcttt acccggggtt cttcgactac
      181 tacatcatct atcagcattt tgaacgtttc ctggaaaaag gcttcaataa tccctacccg
      241 ttctggtttt acatccccgt gttgcttggt ctgaccctgc cctggtcgct gcagctctgg
      301 cgtgtcttca acaagacata ttggtctgat tcggcttatg gcgcagttcg gggtctgatg
      361 ttgtcctggt tgctggtggt gctgattttc ttttcggtgc catcgtccaa acttgtcggg
      421 tatgtgttgc ccgctttggc cccgttcgcc ttttttttgg ccgaaccttt cgcggctcgc
      481 ctgcggggcg ccggcgctgc cagggcatta actaccttga ccggttttct tctcgtttca
      541 atcgttattt gtgttgccgc ggttactact ctgatcgtgg cgccgcagcc cagctcccgg
      601 gctttggccg gccagatgct gacgagctat cgcccggccg acaaagtcgt gatgctgaat
      661 cgctatagct atgacctggg tttttacctg cacaccagca aatcggtgtt tgttgtgtcg
      721 ggctgggaca accccgacct gaaattccag gataactggc gcaaagagct tttcgacgca
      781 ggccaattcg agccggacac ggcggcgcgc ctgctgatca agccgcaaga cctggttgac
      841 aagctttgtg cagatcgagt tattaccttg tggttatggg gcgacaaaaa tagcgtcgct
      901 gattatccat ttttggcgtc tatttcgccc tatgcggccg atggtaaatc gcgcgtctgg
      961 cgcattcagg ccggccccgc gcttagtttt tgcgccgaaa ggcccaaaag cggccaagaa
     1021 taaaggtcat tacggcgatc gcgattagaa tcagggcaag caggacgtcg tagcgcaggg
     1081 tggtggcatg cagtaaatag gcataggcca attcattaac gccaaaacct agcgccgaca
     1141 cggcaaagaa gcgtcgcagc gctctggaca ggggcgcgtg ttgcaatttg aaagttaatt
     1201 ggtaatgtcc tagaaacgaa acgcaaaacg cgatcagcca gccaatcaca ttggctatca
     1261 agggcgcata tcccgcaaag ctgaccacgg caacgacgac gagccaatgg gttgccgcag
     1321 cggcgcagcc aaccgcgaca aaccaacaga gctgtttcat gattttctgc ataggcaatc
     1381 ttccggggag gccaggtggt taaggttaaa gagaagcaca ttgtgatatg cgccgatgat
     1441 tttggcatgg attcaggcat tgatgacggc atattgcgcc tggcgcagct aaaacgcctg
     1501 aatgcaacca gttgcctggt tgaaggcccg tcgtttcgca ggaacgcggc ggcgctgaac
     1561 gattcggctt tgcaacttgg cctgcatttg aattttaccg aatcgatggg cgaggccggc
     1621 ttgtacctgc cggtgtcttc gctgattgtc cgcagctatc tgcgccggct tgatgacgcc
     1681 caggtgcatg cccaaatcgc ccgtcaactc gaccttttcg aggcgagctt cggtcgcgca
     1741 cccgattttg tcgatggcca tcagcacgta caccaattgc cgcaaatccg gcaagctttg
     1801 ctggcggaat tgacacgccg ttattcgccg cgcagtccgg cgcccgaagc tggagcgcgg
     1861 gcttgcccct ggttgcgcca caccagagcg ggctctcagt ctggtttgcc tttacgtttg
     1921 cgcctgaagg catggatcat tcagtcgctg ggctcgcgcg ggctgtttca atcggccagc
     1981 tccgccgggt tttcaatgaa tgcgggcctt ttgggcgtgt acgactttcg cggcggggtt
     2041 ataagctatc agacgctgct caagcgatgg cttgaatatg ctgtcgatgg cgatgaaata
     2101 atgtgccatc cggccgccag ggcagtggac ggcgatgtgc tgggcgaaca gcgcgttgcc
     2161 gaattcaatg tgttggcagg cgaccgggcg ggtcaatggc tttgcgatta tgggatagcg
     2221 cttcgagtgt gattgcgagc gcgcttattc agtcgggtgg gtgctgtctc cggccccaag
     2281 cgcgcgctgc atgaacacca catcgatcca ttgcccgaac ttgtaaccgg tcgaaggctg
     2341 tgtcccggcg tccctgaacc ccagacggcg atgcagtgcg atagaggcca cgttgtcacc
     2401 gcccgcgatg acggcaatca tttgccgcca ggggccccgt tcgcagcgtt tgatcagttc
     2461 ctcaagcaaa gcgccgccga atcgcttgcc ggtttggtct tcggccaggt aaattgaatt
     2521 ttcaatcgta tagcgatgcg ctatcctcgg gcggtagggc gccgcgtaac aatagccgat
     2581 taatacaccg tttgcgatcc cgaccaggta gggcaggccg agcattcgaa cgtgttcata
     2641 ccgtgattgc atttctgcga catcgggcgg tttttcttcg aacgttgcag tactatttaa
     2701 tacgtagctc gcgtagatgc gttgcacggc tgacatgtcg tcatgagtcg cgtcacgaat
     2761 ttgcagttgt gttgaatcca tatctttttg cgtcaggatg ggcgctagta taagaggact
     2821 ttccggagaa aagaaatgtc agtttcaatt aacgcgctta cggatgcgtt tgcggtggcc
     2881 ccgcaattat cccttgacga tattgccgac gttgcggcgg ccggctataa aagcgtcatc
     2941 atcaatcgac ccgacttcga gggtggcccc gatcagccga ccgccgccga tatcatggcc
     3001 gccgcaaaaa cggcaggcat ggaggccgag tatctgcccg tggtcagtgg ggcggcaacg
     3061 cggcaagacg cggttcgttt cgcccagttg ctcaagcaat tgccggcgcc ggttctggcc
     3121 tactgtcgca ctgggacccg ttccgcccat ctttatcacg aagcaaatgc gctttaatcg
     3181 atgaacggcg taggcgtatt cacggacttt actaacctgg tatttatagt aatctaaaca
     3241 ttatgagtgt agatagcaat caggaggatc cggccatgtt taaaagaata cttataccga
     3301 ccgacggttc agcactatcc gcgcaagcag caaacaaggg cgtttgtttt gcccgtcaga
     3361 ttggagccga agttgttgcc ctgtatgttg ttcagccgtt tgctgcgacg gtcggcttcg
     3421 atggcatggc ggcggcttat gcgatcaccg acgaagacta tgacaagacc gcggccgaac
     3481 aggctcagaa gtatttgaaa gcggtgctgg atcgcgccat cacggcgggc gttaaagcaa
     3541 ccggtcggac ggtgtccaat ttcaacgtgg ccgatggtct ggtgcaagcg gccgaagaag
     3601 aaaaatgtga tctgattttt attggcagcc atggccgcag cggcctttcc aggctgcttc
     3661 tgggtagtgt tactgtcaag gttctgggct tggcgaagac ggctgttttg gtctatcgtg
     3721 tcaaagagtc ggaataatca agccttgtaa cccggcgtat gctcgctgcc tccggcggcg
     3781 agccgggcgg ttctgaattg ccgcggtcat gcaaggtttt ttacttcatt atgaaaaagg
     3841 tagttttctt gccggatcgc cgcttcagcg ctcagcctga agctgcgcca acgtttgcgc
     3901 ctgttccggg tcagcctgtt cttgataccc gcaacaggcc gttgcgcgac atgcgaattt
     3961 cggtcactga tcggtgcaat ttccgctgta catactgcat gccgcgcgaa gtttttggca
     4021 gcgactacca gttcatgcct cacgcatcgt tgctgacctt cgaagagatc acacgtatcg
     4081 tgcgcgcaag cgtggctctt ggggtggaga aaatccgcct caccggcggc gaaccgctgc
     4141 tgcgaaaaaa ccttgaaacg ttggttgaaa tgctggcgcg tctgcaaacg ccgcaaggaa
     4201 agcccgttga cctgacgctt acgacgaatg gcaccttact ggcgaaaaag gcgcaggcgc
     4261 taaaggatgc gggcctggcg cgggtaacgg ttagtctgga tgcgctggac gagaatctct
     4321 tcgcgcgcat gagcgatagc caggttggcg taaagacgat cttgcgcggc atcgacgagg
     4381 cggcgcgcgt aggccttgct ccggtcaaaa tcaatatggt ggtgcaaaaa ggggttaatg
     4441 aagatcagct tgtcccgatg gcgcgtcatt ttcgccattc gggtcatatc ctgcgcttta
     4501 tcgaattcat ggacgtcggc acgacgaacg gctggaatct tgcgcaggtg ctgacgggag
     4561 cacaaatcct gaagattgtg ggtgccgaat ttccactcga ccctgtaccg gcggcgtatc
     4621 ggggcgaggt ggccgtgcgc tggcgttacg tcgaccaggc cggcgaaatc ggcgtgatca
     4681 ccagcgtgtc gcagcctttc tgcgccgact gcactcgcat acgcctttcc cccgacggca
     4741 agttgttctt gtgtttgttt gccacgcatg ggcacgacct gcgcgcgctg gttcgcggca
     4801 atgcaagcga cgaacagatt gccgcccgtc tggctactgt ctggatgggg cgcgatgatc
     4861 attattccga gcttcgcggc aaggaaacat tgaaagagaa gaaaatcgaa atgagctata
     4921 tcggcggata attgccagat tgacgggctt cattatttca ggaaaaacat gaaattcggg
     4981 tggcaaaaac aaatggccgc gatcggcctt gctgcggtta tgtgggccgg gtctctatcg
     5041 gtgggcatgg ctcagtccgc gccggcggtg ggcgatacgg tgaccgtgcc tgcggtcact
     5101 cttattgatg ggcaaagatt gccggcgggc tattttcagg gcaaaccgct gatcgtggag
     5161 tactgggctt catggtgtcc gttttgcgga cggcagaatc cgcgcttgca gaagctgacg
     5221 cttgaagcgc aaaaaaaggg cttgcaaata ctgacagtca gtgtcgatcg caaggaatcg
     5281 gacgccagtg actatattca ggaacaccat tacacgtttc cggttgccat ggaaacgcct
     5341 gaattgctga agacatttgg acggcgcgat atcattcccg agatcttcgt ggtgacggcc
     5401 aatggcaaac ttgccgaggt tctatcgggc gaattgcccg aagcacaggt cgccggcctt
     5461 atcaagtacg cgccaggcgc gcactagccg gctaatcgct ttcccattgc gtggccttga
     5521 cgaaatcggc ctcgtgttgc tgcacccaga aacgctcgcc gctggcgagc gtttcgcgtt
     5581 tccagaacgg cgcgcgcgtc tttaatgcgt cgataatata ttgacacgca ttaaatgcat
     5641 cgccgcgatg cgcgcatgcg acggcgatga acacgatttt ttctttataa cgcaattctc
     5701 ccacccggtg catgacgact gcgccaagcg tattccaacg tgtttgcgcc gtcgcgcaaa
     5761 ttgactcaat ctcgcgttcg cacatgcctg gataatggtc cagatacaaa gattgtgtcg
     5821 tcttgttgga ggcataatcg cgcacataac cggtgaacgt gacgatcgat ccggctttgc
     5881 cggcggtgcg ttcgcgcaac tgcgcaacaa gttcagcttc gttaaaatcg gcttcctgca
     5941 cgatgaccat tgctttaacc tccggtcacg ggctcgaaaa cagcgacttc gtcgcccggc
     6001 gcgattaaca tgtcgtgggc aatgtggcgt tgattaacgg ccagtttaag gcgccttgcg
     6061 ggagccagtt gcgggtaacg cgattcaagc tggcgcagcc atgccgcccc cgtgactgcc
     6121 tgcatgagcg gccacgactc ctggcgaacc tgaaccagtt ccgccacttg cgcaaaataa
     6181 agtactttaa ttgcagcgcc actcaccgct tttgcctcct gatttgtagc tcagttttat
     6241 ggtttccaga atgattcctt tgtcggcggc tttgcacatg tcataaatcg ttaacgcggc
     6301 aacgctgcat gctgtcatgg cttccatttc gacgcctgtt ttatagttgc tgcggcaggt
     6361 ggccttgatt gtgatgcagc tactcttctc gtcaagtgaa aattcgatac ccgcaaaact
     6421 gagcggcagg ctatggcata agggaatcag ttcggggcag cgtttggcgg ccagcacacc
     6481 ggccacgcgc gcggtgttga gaacctcgcc tttgctgttt tctgccgctg tcagcagcgc
     6541 ataggcggcg ggactcatgc gcacgcgccc gaaagcgatg gcgacacgct gcgaaggcgc
     6601 cttgtcgctg acgtcaacca tgcggatttg tccggattca tccaggtggc tcagtttcag
     6661 ttcgcttgat tcggatgggt tcatagtatg tccgtttagc gaaaaattgc ccggcgggtc
     6721 attaaacaga taactcaatc atatacgcaa taaaccccgg ccgggagccg gcgcattatc
     6781 ctgtttgaag ataaaatgcg tcgcatggtc gtatgattac actctcgagt tcggggtcgt
     6841 gattggcgct tttatttcct ataacggaga cagtaatgac aattaaagta ggcgaacgtg
     6901 ttccggaatc cactctggcg gaatttattg aaaccgagac cgaaggttgc agcctggggc
     6961 ctaataactt tcaggtttcg tcattggtca aaggcaaaac gattgccctg tttgccgtgc
     7021 cgggggcttt tactcccaca tgctcaaaca agcatttgcc cggttttctc gaggccagcg
     7081 ataagctgaa ggcaaaagga gttgacgaga tttggtgcct ggcagtgaac gatcccttcg
     7141 taatgggcgc gtggggcagg gatcgtcaag tcaatggccg cattcgcatg atggcggacg
     7201 gtagcgccgc ctggaccaaa gcgctcgggc ttgaacttga tctggtcggg cgcggcatgg
     7261 gcgtgcgctc gcagcgttat tccgccattt tgaaagatgg catagtgcgg catcttaatg
     7321 ttgaattacc gggcaagttc gaagtcagcg acgcaaaaac tttgttgggt cagctttagc
     7381 gctctcttca agaactttga ttttgcatgt tgaaagcgat cgccgcattt tcatcccttt
     7441 atgcggcgac actcttgttg ctgctgggaa ccgggctttt taatacttat cttggcctgc
     7501 gtctgaccgc aagttccgtg tccgaggtct gggtcggcgc gatgattgcg gtgtattacc
     7561 ttggcctggt ttttggcgcg cgattcggcc acactttaat cgttcatgtc gggcatattc
     7621 gcgcttattc cgccagtgcc gcgattgcta ccgtctcggt gctggccctg gcgctgctga
     7681 acagtttgtg ggtttggctg gcatttcgtt ttattgcagg cattgccatg gtgacgcagt
     7741 tcatggtgat cgagagctgg ctgaacgaac agacggaaaa tcaggttcgt ggccgtgtct
     7801 ttgcctttta catggtgttc tccggtttgg gcactgtttt ggggcaattg tctttaaccc
     7861 ttttcccgcg gctggattat cagcctttga tcttcgttgc catttgctcc gcgcttgggc
     7921 tggtgccagt ggttttgacc cggcgcgttc accccgcctt gcagaagccc gcgcccatca
     7981 tgatcaaata ttacatgtcg cgcgtgccgc tttctttggt ggtgctgttc atggcgggaa
     8041 ccatcatcgg cgctttctat gggctggcgc cggtgtacgc cgccaagcat gcactgtcca
     8101 gcgatcaggt ggcgattttc ctggcggttg cagtggcatc gggcctgatg tcgcagtggc
     8161 ctttgggctg gctggctgat cgcctgaatc gcgctgtcct gattcgcgtg aacgccggcc
     8221 ttttatgcat gttggggatc cccatgtggg gctggtggga gttccccttt tgggcgctgg
     8281 ttgcgttttc gtgcgtgttt ggcattttgc aatttacctt gtacccgctg ggcgtggcct
     8341 ttgccaacga taatattgat cctgaacgcc gggttggatt aagcgcaatc ttgtatatgg
     8401 tctatggatt aggggcttgc gccggacctt ttatcgtcgg tgtgtttatg cgccagttca
     8461 gcgtcgatat gttttatgtt ttcgtctcgt tttgcgcggc ggtcctggtc attttcgtgc
     8521 ggcctgaacg ggtgaccggc gcgaatcgca gcaacgatgc gccgactcag tttgtgccga
     8581 tgtccgatag cttgcaaagc tcgcccgtgg cggcggtgct ggacccgcgc gtcgatgcgc
     8641 aaagcgatat ttccagtgat cccgccgtca gcgcccggta gatcgagcgc gccagaagca
     8701 atcagcgcgg aatcagcacg ataatggccg tgatggccag tgcgatgcca agcacgttga
     8761 cccaggacag tttttctttg aacagccccg cgccgaccaa agtccctacg gaaataacac
     8821 ctatgttcat ggcggaaaac accagcgtcg ggttgtgtgg aaagacttgg tgcgcgcgaa
     8881 tatagaaata gatgttgccg aagttcagta accccagcac gatgcccgca acgatatttc
     8941 gcagactcca ttgggtgcgt tgaataacaa gcaccagaaa gatgacgatg ccggcaagaa
     9001 cg
//
LOCUS       DATCXU010000195         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_657210,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000195 DATCXU010000000
VERSION     DATCXU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657210"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VIR04_04360"
     CDS             <1..1492
                     /locus_tag="VIR04_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY9460013.1"
                     /translation="SRPRMSHIGRAVARNFYAAASNDRLTASWPSTPLTADQIIDRNQ
                     RTLVARSREQAANNDYMRAFLRMSRQNIVGPQGVTLHAQIRNARGALDKNANEILEAG
                     FFQWGHRANCDVTGKRSWRGIQAACAQQAAKDGEFMVRLVYGAEAGPWGFALQTLDPQ
                     RCPVDFNEDRPAGGGFIRQGIHFNRYGRPLWVYFSTTDESESDYRFGGRHYVRVPAAD
                     IVHGFREDMEGQKRGLPWGSTGLFRMRHLNGTEDAAIVNARVGASKMGFIQWKDGHGP
                     EFDEDDDLEISAEPGEFPVLPEGAELKEWAPQYPNGEFAPFTKYLLRGISAGMGVAYN
                     NLANDLEGVNFSSIRQGTLDEREHWKELQEWLIESLIQPVFEAWLPRALLSGRLPGLK
                     PAKIDVYKQQVAWQPRRWQWIDPRADVDGAVATKNNLLASPGQIIREQGRDPQTVWAE
                     SARDVRAMIDAMTAEGIDEKTAKELVLLSMGKAPPKPQQEAKPKGE"
     gene            1489..3459
                     /locus_tag="VIR04_04365"
     CDS             1489..3459
                     /locus_tag="VIR04_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY9460014.1"
                     /translation="MMKRELQKRLAEIQGTRDLKTTAKVASIDVEARTVELAFSSEAE
                     YRRWWGIEVLGHEPGEVDLSRLNDGAAVLWNHNWDTQVGVVESARVDSDKKGRATVRF
                     SRNARADEIFQDIVDKIITKVSVGYRIQDAKLIEERDGEEVWRVTDWQPYEISPVSVP
                     ADASVGIGRSMEAENPQEESAAPPAQNSTVHKGGIQLSIEERAEMKIKTVRNAAGHLV
                     RAEVDEADNDKIVRELEVIEYAGAERAAGEQAERSRVNAITEMGKRYGATDLALEFVG
                     NGQNADAFQRALLERMNERKAKPLADQAQPSSQIGLTDTEARSYSLMRVVRALANPND
                     ARLRKDAAFEFECSEAAQRQFGREAKGVLIPEDVLARAFNAGGAANLPVGSTTGSELV
                     ATTLMAGSFIEALRHRTTIMRLGFSMAGLVGNVEIPKQTGGATAYWVGEGEDATEGVP
                     TIGQIALSPKTVAAYTDITRKLMQQSTPDAEGIVRRDLVNAIAQALDYAGYYGTGVGN
                     QPKGIKNYTGINSVDFAVANPTFAELVSMESAIAADNADVNSMAYVANAKFRGHCKTT
                     PKFGAGTEATIWEPGGTVNGYRSEITNQVADGDVFFGNFSDLIVALWGGLDLTVDPYS
                     LSKSGGTRLVVFQDADFVLRREESMCYGVLIP"
BASE COUNT          813 a         1004 c         1189 g          608 t
ORIGIN      
        1 gagccggccg cgtatgtcgc acatcggccg cgcggtcgcg cgcaacttct atgcggccgc
       61 gtcaaatgat cggctgaccg caagctggcc gagtacgccg ctgaccgccg accagattat
      121 tgatcggaac cagcgcacgc tggtggcgcg ttcgcgcgag caggcggcga acaacgacta
      181 tatgcgcgcc ttcctgcgga tgagccgaca gaacattgtc ggcccgcaag gcgtgacgct
      241 gcacgcacaa attcgcaacg cgcgcggcgc gctggataag aacgccaacg aaatactgga
      301 agccggtttt tttcagtggg ggcaccgcgc caattgcgac gtgaccggga agcggtcttg
      361 gcgcggcatc caggccgcct gcgcgcagca ggctgctaag gatggtgaat tcatggtgcg
      421 cctggtctat ggcgctgagg caggaccgtg gggcttcgcg ctgcagacgc tggacccgca
      481 acgctgtccg gtggatttca acgaggaccg gcccgccggc ggcggtttca ttcgccaggg
      541 aatccatttc aacagatacg gacggccgct gtgggtgtat ttcagcacga ccgacgagag
      601 cgaatccgac taccgtttcg gcgggcgcca ttatgtgcgc gttcctgcgg ccgatatcgt
      661 ccacggattc cgcgaggaca tggaaggaca aaaacgcggg ctgccttggg gttccactgg
      721 cctattccgc atgcgccacc tgaacggcac cgaggatgcg gcaatcgtca acgcgcgagt
      781 gggcgcgtca aaaatgggct ttatccagtg gaaggatgga cacggccctg agtttgatga
      841 ggacgacgat ttagaaatca gcgcggagcc tggggaattc ccggtgctgc cagagggcgc
      901 ggagttgaaa gagtgggcgc cgcagtatcc aaatggcgag tttgcgccat tcaccaaata
      961 cctgttgcgg gggatttccg ccggcatggg cgtggcctac aacaacttgg ccaacgacct
     1021 ggaaggcgtc aacttttcga gcattcgcca gggcacgctg gatgagcgcg aacactggaa
     1081 agaattacag gaatggctga ttgaaagcct tatccagcca gtgtttgaag catggctgcc
     1141 gcgcgcgctg ttgtccgggc gtttgcctgg tttgaagccc gcaaaaatcg acgtttataa
     1201 gcagcaggtg gcatggcagc cgcgccggtg gcagtggatc gacccgcgcg cagacgtgga
     1261 cggcgctgtg gcgacgaaaa acaacctgct tgcatcgccg ggccaaatca tcagagagca
     1321 gggccgcgac ccgcaaaccg tatgggccga atcggcgcgc gatgtgcgcg caatgatcga
     1381 cgccatgacg gccgaaggca tcgacgaaaa aaccgcgaaa gaattggtgc tgttgtcaat
     1441 gggcaaagcg ccgccaaaac cgcaacaaga ggcaaaaccg aagggcgaat gatgaaacga
     1501 gaactgcaaa agcgcctggc agaaatccag gggacgcgcg acctaaagac cacggccaag
     1561 gtggcgtcca tcgacgtgga agcgcgcacg gtggaactgg cgttttcgag cgaggcggaa
     1621 tatcggcggt ggtggggcat tgaagtgctg ggccatgagc ctggcgaagt ggacttatcg
     1681 cgcctgaacg atggcgcagc ggtgctctgg aaccacaact gggacaccca ggtgggcgtg
     1741 gtggaaagcg cgcgcgtgga cagcgacaag aagggccggg ctactgtccg cttttcgcgc
     1801 aatgcgcggg cggatgaaat ttttcaagac attgttgaca agatcatcac caaggtatcg
     1861 gtgggctatc gcatccagga tgcgaagttg atcgaagaac gcgacggcga agaagtttgg
     1921 cgcgtgaccg attggcagcc ctacgaaatc agcccggtat ctgtgccggc ggacgcatcg
     1981 gtggggattg gtcgaagcat ggaagcggaa aacccgcaag aggaaagcgc ggcgccacct
     2041 gcgcagaatt caaccgtcca caaaggtggc attcaacttt caattgagga aagagcagaa
     2101 atgaaaatca agaccgtgcg caatgccgcc ggccacttgg tccgcgccga agtggacgag
     2161 gccgacaacg ataaaatcgt tcgggaactg gaagtcatcg aatacgccgg cgccgaacgc
     2221 gcggcaggcg agcaggcgga acgcagccgc gtcaacgcca tcaccgaaat gggcaagcgt
     2281 tatggtgcta cggacctggc gctggaattc gttggcaacg gccagaacgc ggacgcattc
     2341 cagcgcgcgt tgctggaacg catgaacgag cgcaaggcca agccgctggc cgaccaggcg
     2401 cagccgagtt cgcaaatcgg actgaccgat accgaagcgc gttcctattc gctgatgcgc
     2461 gtagttcgcg ccctggcgaa tccgaacgat gcgcgcttgc gcaaggacgc cgcattcgaa
     2521 ttcgagtgca gcgaagcagc gcaacgccag tttggccgcg aagccaaagg cgtgcttatc
     2581 cccgaggacg tgctggcgcg tgcattcaac gccggcggcg ccgccaactt gccggtgggt
     2641 tccacgaccg gcagcgaact ggtggctacc actttgatgg ccggttcctt tatcgaggcg
     2701 cttcgccatc gcaccaccat tatgcgcctg ggcttttcca tggctggcct ggtgggcaat
     2761 gtggaaattc cgaagcagac cggcggcgca actgcgtact gggtaggtga gggcgaggac
     2821 gcgaccgaag gcgtgccgac catcggccag attgcgctgt ctcccaagac ggtggcggca
     2881 tataccgata tcacgcgcaa gctgatgcag cagtccacgc cggacgcgga gggaattgtg
     2941 cgccgcgact tggttaacgc cattgcgcaa gcactggact atgccggcta ttacggcacc
     3001 ggggtgggca accagcccaa ggggatcaag aattacaccg gcatcaattc ggtggacttc
     3061 gcggtggcaa atccgacatt cgcggaactg gtttcgatgg aaagcgcaat cgcggccgat
     3121 aacgcggatg taaacagcat ggcctacgtt gccaatgcga agttccgggg ccactgcaag
     3181 accacgccga aatttggcgc tggcaccgag gcaaccattt gggaaccggg cggcaccgtc
     3241 aacggctatc gttcggaaat caccaaccag gtggctgacg gcgacgtgtt tttcggcaac
     3301 ttcagcgact tgattgttgc gctgtggggc ggtctggacc tgacggtgga cccgtacagc
     3361 ctgagcaaat ccggtggcac ccgcctggtg gttttccagg acgcggattt cgtgctgcgc
     3421 cgcgaggaaa gcatgtgcta cggcgtgctg attccgtaaa accagcgagc agtagcaata
     3481 cccgaaaggg ccgccaggac ggcggccctt ttttacaacc aaagcgacga ggaatcgagc
     3541 atggcaaaga agaaagagaa agacgcaccg gccggcgtaa tggtcaaggc tgtttctggc
     3601 ttcatggtgg acgg
//
LOCUS       DATCXU010000196         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_441044,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000196 DATCXU010000000
VERSION     DATCXU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441044"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VIR04_04370"
     CDS             complement(<1..1128)
                     /locus_tag="VIR04_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY9460015.1"
                     /translation="MKSESVVVCGSGIVGLATVLGLTRAGIKTVLLGPRHEPAPMNVD
                     EYCPRVYAISLASQQFLAQLGVWNMLDADRIAAVEAMEVHGDANGQVTLRAWQATQPT
                     LAWIVESSEIERVLQHAVHVFGIAWRDERFQQLEPGAIVTESGQRLEADLVVGADGAN
                     SPVRTAAGINHRSSPYGDVGLVAHLNSEYFHQQTAVQWFTGDSVLALLPLPDTSDGHQ
                     VSMVWSAPQAMASQVLALPEPQRNELLQTHLQQISGGRLGRLQVRSPVFGFPLYLESS
                     AMVAPGVALVGDAAHRVHPLAGQGLNLGLADAAALLHVLGHRESYRGVGERRVLERYR
                     RQRAEAILAMRLATDGLYRLFAARATPVAWARNLGMRGLERV"
     gene            complement(1169..2452)
                     /locus_tag="VIR04_04375"
     CDS             complement(1169..2452)
                     /locus_tag="VIR04_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEY9460016.1"
                     /translation="MKRILFSSLLLVILAACSTAPVNLEQKQAPAPSQAAANIAEQPL
                     RVPPLETMRDTPPRTLAGRFQASSWAALPGWQTDDLSHVWKAFINNCKGLMRPISGSL
                     AIPARATPRAWQPVCAAAANAGISAGATGAIRQFLQAHLQPWHLLDGRGHPAENMITG
                     YYEPTVHASRTRGGNYQWPMYAAPADLLTIDLGSVYPELAGKRVRGKLVGNHVVPYDT
                     RADIAGSARQPPVIVWVNDPVEAFFLQIQGSGRAILSDGSTIRLAYADHNGRPYTSIG
                     KWLADRGEMPLAKASMQNIKAWAKAHPSRVQELLNVNTAMVFFREEPIVDPELGPSGA
                     YGIPLIGQRSVAVDTSFVPLGAPVFLSTQYPASAQPLNRLVFAQDTGAAIRGAARADF
                     YWGSGDEAGALAGRMKQRGRMWVLWPRQAGAPSAR"
     gene            complement(2475..2849)
                     /gene="apaG"
                     /locus_tag="VIR04_04380"
     CDS             complement(2475..2849)
                     /gene="apaG"
                     /locus_tag="VIR04_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY9460017.1"
                     /translation="MKPYDMIVTVTPQYLPDQSEPDEQQFIFAYTVRITNSGKYPAQV
                     ISRHWIITDGEQRVQEVRGLGVVGQQPLLGPGETFEYTSGCPLPTPVGTMQGSYHCVG
                     EDGVPFDVPIGEFVLAMPRTLH"
     gene            2931..3650
                     /gene="rpe"
                     /locus_tag="VIR04_04385"
     CDS             2931..3650
                     /gene="rpe"
                     /locus_tag="VIR04_04385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY9460018.1"
                     /translation="MSTVSDTRIAASILAADFARLGEEVQNVVAAGADWIHFDVMDNH
                     FVPNLTVGPMVCEALRPHTKAPIDVHLMVEPVEALIPRFAKAGANIISFHPETVSHPD
                     RTLTLIREHGCKAGMVFKPATPLDWMDYLMDKLDVVLIMSVNPGFGGQPFIPGTLKKL
                     TQARKKVDAWQTNGGQPILLEVDGGIRVGNIAGVRAAGADTFVAGSAIFGRPDYKAVI
                     DAMRAEIIKGEAAKSETPSVQ"
     gene            3662..4333
                     /locus_tag="VIR04_04390"
     CDS             3662..4333
                     /locus_tag="VIR04_04390"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY9460019.1"
                     /translation="MPFAAVLFDLDGTLLDTIADIADATNAMLADLERSPVSQALIRS
                     YVGKGTKNLIARVLSEHHDGLGCKPDEIERAMALFSRHYGRLNGDKTILYPGALEGLE
                     DFQEQGAKLAIVTNKSAEFTPPLLARMGIDHFFEHVICGDTCTERKPHPMSMLHACEL
                     LRVAPSDTLAIGDSINDARAAQAAKIKVLAVPYGYNEGVDVRTLEVDDIVTSIEAAAR
                     WAAQH"
BASE COUNT          937 a         1373 c         1265 g          844 t
ORIGIN      
        1 cacgcgctca aggcctcgca tccctagatt gcgggcccac gccaccggtg tagcccgggc
       61 tgcgaataaa cgatacaaac catccgtggc cagacgcatc gccagaatcg cttcggcgcg
      121 ctggcgccga tagcgctcaa gcacccgccg ctctcccacg ccccgatagg attccctatg
      181 gcccagcaca tgcagcaagg ccgccgcgtc ggccagcccc aaattcaagc cctgtcctgc
      241 caggggatga acccggtgcg ccgcatcgcc caccagtgca acgcccggcg caaccattgc
      301 gctgctttcc aaatacagag ggaaaccgaa tacggggctg cgcacctgca agcgtcccag
      361 ccggccgccg ctaatttgtt gcaggtgagt ctgcaacaat tcattgcgct gcggttcagg
      421 caacgcgagt acctggctgg ccattgcctg cggcgccgac cacaccatgg atacctgatg
      481 gccatcggag gtgtcgggca aaggcagcag agccagtacg ctgtcgccgg tgaaccactg
      541 caccgccgtt tgctggtgaa agtattcact gttcaaatgg gcaaccagtc ccacgtcgcc
      601 ataaggcgaa gagcgatgat tgatgcccgc tgccgttcgc acgggagaat tcgcaccgtc
      661 ggcgccaacg accagatccg cttccaggcg ctggccggat tcagtcacaa tcgcccccgg
      721 ctcgagttgc tgaaatcttt catcgcgcca tgcaatgcca aatacgtgca ccgcatgctg
      781 caagacacgt tcaatttcgc tggattcaac aatccaggcc aaagtgggct gcgttgcctg
      841 ccaggcccgc aatgtcacct gaccatttgc atcgccatga acctccatgg cctcgaccgc
      901 cgcaatccgg tccgcgtcaa gcatgttcca gacaccaagc tgcgccagaa actgctggct
      961 ggcgagcgaa atggcgtaaa cccgcggaca atactcatcg acattcatgg gcgccggctc
     1021 gtggcgcggg ccaagcagta cggtcttgat gcccgcgcgc gtcaaaccca gtacggtcgc
     1081 caggccgaca atgccgctac cacatacgac cacagattct gacttcatcg gaactcccga
     1141 gtaaccccgg cgcccggccg ggcgcatgtc accgcgccga tggcgcgccc gcttgcctgg
     1201 gccacaatac ccacattctt ccgcgttgct tcatgcggcc ggccaaggct ccggcctcgt
     1261 cgcccgagcc ccagtagaag tcggcgcgcg ccgctccgcg gatggccgcg ccggtatcct
     1321 gcgcaaatac cagacggttc aatggctggg ccgacgcggg atactgggtg gacaggaaaa
     1381 ccggcgcgcc caatggcaca aacgacgtat cgaccgccac cgacctttga ccgatcagcg
     1441 gaataccata cgcaccgctg ggccccagtt cggggtccac aatcggttcc tcgcggaaaa
     1501 acaccattgc ggtgttcacg ttcaacagct cctgcacgcg cgacggatgc gccttggccc
     1561 aggccttgat attttgcatg gacgctttgg ccagcggcat ttcgcccctg tcggccagcc
     1621 atttgcctat cgatgtatag ggccggccat tatggtcggc atacgccagg cgtatcgtcg
     1681 agccgtcgga caagatggcg cgcccggagc cttggatttg caggaagaag gcttcgaccg
     1741 ggtcgttgac ccacacaata acaggcggtt gtcttgccga tccggcaata tcggcgcggg
     1801 tatcgtacgg tacgacgtga ttgccgacca gttttccgcg cacgcgcttg cccgcaagtt
     1861 cgggatagac cgagcccaga tcaatggtaa gcaaatcggc cggcgcggcg tacatgggcc
     1921 attggtaatt cccgccgcgc gtgcgcgaag catgcaccgt cggctcatag tagccggtga
     1981 tcatattttc ggccggatgg ccccgcccgt cgagcaaatg ccagggctga aggtgcgcct
     2041 gcaaaaactg gcggatcgcg ccggtcgccc cggcggaaat tcccgcattt gccgcagcgg
     2101 cgcatacggg ctgccaggcg cgcggcgttg cacgcgcggg gatggccagg gatcccgata
     2161 tcggacgcat cagccctttg caattgttga tgaacgcttt ccagacatgg cttaaatcat
     2221 cggtttgcca gcccggcaaa gcagcccagg acgaggcttg gaatcgtccc gccaacgtac
     2281 gcggcggggt atcgcgcatg gtttcaagcg gcgggacccg caagggttgt tcagcaatat
     2341 tggcggcggc ttgagacggc gcgggggcct gtttttgttc aagattgacg ggggcggtgg
     2401 agcaagctgc cagaataacc agcagcaaag aagaaaacag aattcttttc ataaagtccg
     2461 gcaaaaatta ctgattaatg caaagtgcgc ggcatcgcca gaacgaattc gccgatggga
     2521 acatcgaaag gcacgccatc ttcgcccacg caatggtacg agccttgcat cgtacctacg
     2581 ggagtgggca gagggcaccc gctggtgtat tcgaatgttt cgccgggtcc cagcaaaggc
     2641 tgttgtccca caacgcccag tccgcgcact tcctgcacgc gctgctcgcc gtcggtgatg
     2701 atccagtgtc ggcttattac ctgggccgga tacttgccgc tattcgtaat gcgcacagtg
     2761 tacgcgaaga taaactgctg ctcatcgggc tcggattgat ccggcaggta ttggggcgta
     2821 acagtaacga tcatatcgta aggtttcaca ggtcgggtcc atagtcagac tataatgaaa
     2881 gttcgttgca tacgatttta ttcttgaacc agggattcgt tttcacgtcc atgtccactg
     2941 tttccgacac ccgcattgcc gccagtatat tggccgccga ttttgcccgg ctgggcgaag
     3001 aagttcaaaa cgtcgtcgct gcgggcgccg actggataca cttcgacgtc atggacaacc
     3061 attttgttcc caacctgacg gtaggcccca tggtgtgcga ggcgttgcgg ccccacacca
     3121 aagcccccat cgacgtgcac cttatggttg agccggtcga agcccttatt ccgcgctttg
     3181 ccaaagccgg cgccaatatt atttcgttcc accccgaaac ggtgtcccac cccgaccgca
     3241 ccctgaccct gatccgggaa cacggctgca aagccggcat ggtgttcaaa cccgccacgc
     3301 cgctggactg gatggactac cttatggaca agctggatgt cgtgctgatc atgtcggtaa
     3361 atcccggctt cggcggacag ccttttatcc cgggcacgtt aaagaaactg acacaggcgc
     3421 gtaaaaaagt cgacgcctgg caaaccaatg gcggtcagcc cattctgctg gaagtcgacg
     3481 gcgggatcag ggtaggaaat attgccggcg tccgggctgc cggcgccgac acttttgttg
     3541 cgggatcggc cattttcgga cggcccgatt acaaagcggt cattgacgcc atgcgggccg
     3601 agatcatcaa aggtgaagcg gccaaaagcg aaactccctc cgttcaataa caggattctc
     3661 aatgccgttt gccgctgtat tgtttgatct ggacggcacc ctgctggaca ccattgccga
     3721 tatcgccgac gcgaccaatg ccatgctggc cgatcttgaa agatcgcccg tatcgcaggc
     3781 gctaatacga agctacgtgg gcaagggcac taaaaacctg attgcgcgcg tcctgtccga
     3841 acatcacgat ggtctcggat gcaagccgga cgaaatcgaa cgcgcaatgg ccctgttcag
     3901 ccgccattat ggccggctca acggcgataa aactattttg tacccgggcg cgctggaagg
     3961 ccttgaagat tttcaggagc aaggggcaaa attggcgatc gtcaccaaca agtcggccga
     4021 attcacgccg ccgctgctgg cgcgcatggg aatcgatcat ttcttcgagc atgttatttg
     4081 cggcgacaca tgcaccgaac gaaaaccgca tcccatgtcc atgctgcacg cctgcgaatt
     4141 attgcgggtc gcaccgtccg acacgctggc cattggcgat tcgatcaatg acgcaagggc
     4201 ggcgcaagcc gcgaagatca aagtgttggc tgtgccctac ggctacaacg agggcgtcga
     4261 cgtgcgaact cttgaagtcg atgatatagt tacgtccatt gaagctgcgg cccggtgggc
     4321 cgcgcaacac taaaacaacg cactggcgca caccatgtcg actaccccct attgctacgc
     4381 taccggccgc gccatctggc gctggtggcg cttgtgtcc
//
LOCUS       DATCXU010000197         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_435077,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000197 DATCXU010000000
VERSION     DATCXU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435077"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VIR04_04395"
     CDS             <1..522
                     /locus_tag="VIR04_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY9460020.1"
                     /translation="LPPRAPFISLARAVIGQQVSVKSADATWKRFTGKCCGEPTPAAV
                     LKVSAEELREAGLSKRKAEYVHDLATHFLEERVRPSLWADKDDEAIVAELCAIRGVGR
                     WTAQMFLIFSLQRPDVLPLDDIGLIKAISLHYFSGEPVSRFEAREVAQAWAPWRTVAT
                     WYLWRSLDAIAIR"
     gene            559..1524
                     /locus_tag="VIR04_04400"
     CDS             559..1524
                     /locus_tag="VIR04_04400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY9460021.1"
                     /translation="MRNTFLEFEQPLAELENKIEQLRFVQTDSAVDISDEVGRLQQKS
                     QALAKSIYSKLTPWQTALVARHPQRPYTFDYVREIFTDFHEMHGDRMYADDLSIVGGL
                     ARFHGTPCMVIGHQKGRDTKERAMRNFGMPRPEGNRKALRLMRLAEKFRLPVFTFIDT
                     PGAYPGVGAEERGQSEAIGHNIYAMAELRVPIIATIIGEGGSGGALALAVGDVVMMLQ
                     YSTYAVISPEGCASILWRSLDKAPVAAEALAITAPRLKELGLIDRIVNEPVGGAHRDP
                     VVMARQLSRALADSLREVSGLTVDELLEHRLERLMSYGRFQETRA"
     gene            1517..>2060
                     /gene="tilS"
                     /locus_tag="VIR04_04405"
     CDS             1517..>2060
                     /gene="tilS"
                     /locus_tag="VIR04_04405"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY9460022.1"
                     /translation="MPKLASGQPFGSERLVQAIAHSVAALPERPEGLAVAVSGGPDSA
                     MLAVHASIFAQQEPLPLHFFYIHHGLQKPAEPWQAHVHDLAQYLRVPCHSLRIQVDLS
                     RGDGVEAAARDARYAALARLSRQAGVDHILLAHHRNDQAETVLLRLLRGTGPTGLAAM
                     APVARRQGLTYLRPWLNVDRA"
BASE COUNT          372 a          578 c          646 g          464 t
ORIGIN      
        1 ctgcctccgc gcgcgccgtt catctctctg gcgcgcgccg tcattggtca gcaggtttct
       61 gtaaaaagcg ccgatgcgac atggaagcgt ttcacgggga aatgctgcgg cgaaccgact
      121 ccggcggctg ttcttaaggt aagcgccgaa gaacttcggg aagccgggtt atccaagcga
      181 aaggccgaat atgttcacga cctggcgacg cattttcttg aagaacgggt gcgcccttcg
      241 ttgtgggccg acaaggacga tgaagcgatt gtcgccgaac tgtgcgccat tcgcggcgtt
      301 ggccgctgga cggctcagat gtttttaatt ttcagcttgc agcggcccga cgtcttgccg
      361 ctggatgata tcggtttgat caaagctatt tcgctgcatt acttcagcgg cgagccggtt
      421 tcccgcttcg aggcgcgcga agtcgcgcaa gcgtgggcgc cgtggcggac agtggctacc
      481 tggtatctgt ggcgcagcct cgatgcgatc gccattcggt aacggcaatg atcgctctta
      541 atgggacacc aggcacttat gcgtaatact ttcctcgaat tcgagcagcc actggccgag
      601 ctcgagaaca agattgagca gttgcgtttt gtgcagactg actccgccgt cgatatttcc
      661 gacgaagtgg ggcgtttgca gcaaaaaagc caggcactgg ctaaaagcat ttattcgaaa
      721 ctcacccctt ggcagaccgc gctggtggca cgccatccgc aacggcctta tacgttcgat
      781 tatgtgcgtg aaattttcac cgatttccat gaaatgcacg gtgatcgaat gtatgccgac
      841 gatctgtcca ttgtcggcgg cctggcccgc tttcacggca ctccgtgcat ggtgatcggg
      901 catcagaaag gccgcgacac caaggaacgc gccatgcgca attttggcat gccgcgcccc
      961 gagggcaatc gcaaggcatt gcggctgatg cggctggcgg aaaaattccg cttgccggtt
     1021 tttacgttta tcgacacccc gggcgcctat cctggcgtcg gcgccgagga acgcgggcag
     1081 tccgaggcaa tcggccacaa tatatatgcc atggccgaac tcagggtgcc gattatcgcg
     1141 accattattg gcgagggcgg ttcgggcgga gcccttgcgc tggccgtagg cgatgtggtc
     1201 atgatgcttc agtactcgac ctatgccgtt atttcccccg agggctgcgc gtcgatcctg
     1261 tggcgcagcc tcgataaggc gccggttgcg gccgaagctt tggccattac cgcgccgcgt
     1321 cttaaagagc ttggcttgat cgatcggatc gtcaacgagc ccgttggcgg cgcgcatcgc
     1381 gatccggttg taatggcgcg ccaacttagc cgcgcgctgg cggattcgct gcgggaagtg
     1441 tcaggcctga cagtcgacga acttttggag catcgtctgg aacgattgat gtcgtatggc
     1501 cgattccagg agacgcgtgc ctaagctcgc atccggtcag ccgtttggct cggagcggct
     1561 tgttcaggcg attgcacact ctgttgcggc cctgcccgag cggcccgaag gattggcggt
     1621 cgccgttagc ggaggccccg attccgccat gctggctgtg catgcttcta tttttgcgca
     1681 acaggaaccg ttgccgctgc atttttttta tattcaccat gggctgcaga agccggccga
     1741 gccatggcag gctcatgttc atgatttggc tcaatacctg cgcgtgccct gtcatagtct
     1801 tcggatacag gttgatttat cgcgcggcga cggcgttgaa gccgccgcgc gcgatgcgcg
     1861 ttatgcggca ttggcgcggc tctcgcggca agcgggcgtt gatcatattt tgctggcgca
     1921 tcatcgcaac gaccaggccg agacggtgtt gctgcggctg ttgcgcggaa ctggaccaac
     1981 cggcttggcg gccatggcgc cggttgcgcg aaggcagggt ctgacttatt tgcgtccgtg
     2041 gctgaatgtg gatcgcgcgg
//
LOCUS       DATCXU010000198         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_409949,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000198 DATCXU010000000
VERSION     DATCXU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409949"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VIR04_04410"
     CDS             complement(<1..363)
                     /locus_tag="VIR04_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEY9460023.1"
                     /translation="MSIASAFGGKNKEDARNASFVAFVADDASTQEVQQVVAHLGIPN
                     ALVARGGIDAAIDHLGHVEKPPQRLVVDISGIDLPLLALGRLADACDPSVQVYVLGDQ
                     NDVRLYRTLLQAGVHDYLV"
     gene            complement(389..856)
                     /locus_tag="VIR04_04415"
     CDS             complement(389..856)
                     /locus_tag="VIR04_04415"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEY9460024.1"
                     /translation="MTSLPLNLFPTRGAQKPVRGRSQRARCAKLCLIISFGMVLTACA
                     FPRKDFSSMPDPGVISVQQTGQGYKAVAPDCGRLLQPSQYNKADDLRMSIAFGCATYT
                     NLAGQLARPEDLIAPKSYAGQSADTAGDAVTRYRENKVTPLRETMSTNAGKQH"
     gene            complement(888..>2239)
                     /locus_tag="VIR04_04420"
     CDS             complement(888..>2239)
                     /locus_tag="VIR04_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY9460025.1"
                     /translation="KQQANAPVSASNQSPALSVGEGRLIRFRGAAKSVFVADPEIADV
                     QVPSEGAVLVLGKKTGQTTLYVLGDNGQTLVQQRITVRHNTPEMEEVLHQRFPGLRVS
                     LESSPGSLMVSGTVPNSETIDAITRTLKSYLGAKEQLINRLAISSPTQVYLRVRVTEV
                     SRAVTQKLGINWSAITSPSNFFAGVISGRAIQSITSSFVGGDKGLQFASSGTYSAAQQ
                     GGYSFLGGYAKGGQSIQTMIDVLDQEGMISVLAEPNLTAVSGETASFLAGGEFPIPVR
                     QDHDSMTVEFKPFGVALDFTPTVLGDDRISIKVRPEISEIDPNSSILMQGISIPGLSV
                     RRVETTVEMASGQSFAIGGLLQNNIRDIVSQVPGLGKIPILGKLFSSSDYQNNKSELV
                     IIVTPYLVKPSDSSQTASPLASLRPNTDIEYILHDKIGLDPLKGDVPRLTGSAGFVY"
BASE COUNT          427 a          627 c          666 g          519 t
ORIGIN      
        1 gaccaggtaa tcgtgcacgc ccgcctgtag cagagtgcgg tacaggcgga catcgttctg
       61 gtcgcccagg acataaacct gaacggacgg gtcgcaggcg tcggccagcc tgcccagggc
      121 cagcagcggc aggtcaatgc cggagatatc aaccacgagt ctttgcggtg gtttttcgac
      181 atggcccagg tggtcgatcg cagcgtcgat tccgccgcgc gccaccaagg cattgggaat
      241 tcccaggtgc gcgacgactt gctgcacctc ttgtgtgctg gcgtcgtcgg caacgaaggc
      301 gacaaagctg gcattgcgtg catcttcttt attttttccg ccgaaggcgc ttgctatgct
      361 catggtttct cctttctgtc tgtgtctgtt agtgttgttt gcctgcgttt gtcgacatgg
      421 tttcccgcag cggggtcacc ttgttttcac ggtagcgtgt aacggcgtca ccggcggtat
      481 cggcggactg gcccgcatag gacttcggcg cgatcaaatc ttcagggcgg gcaagctggc
      541 ccgcaagatt ggtataggtg gcgcagccga atgcaatcga catgcgaagg tcgtcggctt
      601 tgttgtattg cgacggttgc agcaggcgcc cgcagtctgg cgcaacggct ttgtacccct
      661 ggcctgtctg ttggacgctg ataactcccg gatcgggcat ggaggaaaaa tccttgcggg
      721 ggaatgcgca ggcggtcagt accatgccaa agctgataat caggcatagc ttcgcgcaac
      781 gggctcgctg cgatcgacct cttactggtt tttgagcgcc gcgggtggga aacaggttta
      841 gcggtaacga tgtcatgatt gtgtgtcctg gcaaagggtg gggtgcgtca gtagacaaat
      901 ccggccgatc cggtaaggcg cggcacgtcg cctttaagcg gatccaggcc gatcttgtca
      961 tgcaggatgt attcgatatc ggtgttcggg cgcaggcttg ccagcggcga cgctgtttgg
     1021 ctgctgtcgc tgggcttgac caggtaaggc gtcacgatga tcaccagttc gcttttattg
     1081 ttctggtaat cggaagaaga gaacaacttg cccaggatgg gtattttccc cagccctgga
     1141 acctgcgaga cgatatcccg gatgttgttc tgaagcagcc cgccaatggc aaagctctgg
     1201 ccgctggcca tctcgacggt ggtttcgacg cgtcgcaccg acagccccgg aattgaaatc
     1261 ccttgcatga gtatgctgct attgggatcg atttcgctga tttcaggccg caccttgatg
     1321 ctgatccggt catcgccgag cactgtcggc gtgaaatcca gcgccacccc gaaaggtttg
     1381 aattccaccg tcatgctgtc gtgatcctgt ctgacgggaa taggaaactc tccgccagcc
     1441 aaaaagctgg ccgtttctcc cgataccgcg gtcaggttgg gttcggccag tacgctgatc
     1501 atgccttcct gatcaagtac gtcgatcatg gtctggatgg actggcctcc cttggcatag
     1561 ccgcccagga atgaatagcc gccctgttgc gccgcgctat aggtccccga agacgcaaac
     1621 tgcaaaccct tgtcgcctcc gacgaagctg ctggtgatgc tctgaatggc gcggccgctg
     1681 attacgcccg caaaaaaatt gctgggggag gtaatggccg accagttgat gcccagtttt
     1741 tgggtcaccg cccgcgacac ttcagtcaca cgcacgcgca gatacacctg agttgggctg
     1801 ctgatcgcca ggcgattgat caactgctct tttgcaccca ggtaagattt cagcgtgcga
     1861 gtaatggcat ctatggtttc gctgttgggc acggtgccgc tcaccatgag cgaccccggt
     1921 gaggattcca gcgaaacgcg caatcccggg aagcgctgat gcaatacttc ttccatttcc
     1981 ggcgtgttgt ggcgaacggt gatgcgctgt tgcaccagtg tctgaccgtt atcgcccagc
     2041 acataaagcg ttgtctgccc cgtttttttg cccagaacca gcactgcacc ttcggacgga
     2101 acttgtacat ccgcaatttc cggatcggcc acgaaaaccg atttcgcagc tcccctgaat
     2161 cgaatcagcc ttccttcgcc aacggaaagg gccggcgact ggtttgacgc agacactggc
     2221 gcattggctt gctgttttg
//
LOCUS       DATCXU010000199         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_483987,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000199 DATCXU010000000
VERSION     DATCXU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483987"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIR04_04425"
     CDS             complement(<1..341)
                     /locus_tag="VIR04_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY9460026.1"
                     /translation="MNITILDDYQDCVRHLNCFSSIAQHNVRIFNDTVKGVDALAERL
                     ADADAVVLMRQRTAIKKELLDRLPKLRMLSQTGAIGAHVDLPACSERGIVVMAGKGDG
                     STTAELTWALML"
     gene            561..1526
                     /locus_tag="VIR04_04430"
     CDS             561..1526
                     /locus_tag="VIR04_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460027.1"
                     /translation="MKKITQTAAAILLATGLSSPSLADTWPDQPIHFIVPYTAGGGAD
                     GVARVVGNKLGKALGQPVIIENRGGAAGNIGTNLAAKADPNGYTIMLGAAALAINETL
                     YKKLPFDPVKDFTPVTLLVKTPNILVVNPKLPVKTVKELIQLARSKPGVLNYASAGKG
                     TTPHLAAELFDSMAGVKMTHIPYKGSSPAVVALVGDEVDVMLSPALTVLPFIKTGRLR
                     ALAMTGATRSTAFPDIPTIAESGLPGYEASQWYAVFAPAGTPADIVARLNRELVKIVH
                     SPDVEKVFALEGSEPIGGTPEELGQYFKSEIARWAKTIPSGERLN"
     gene            1551..2120
                     /locus_tag="VIR04_04435"
     CDS             1551..2120
                     /locus_tag="VIR04_04435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY9460028.1"
                     /translation="MDVDYAGLKKSVANTIAEVKSGSKEPIRRPKARLRVVRGLQGEV
                     TAGKFTYRTDAALDAGGFAEHPRPMDYLLGALASCQQMWCLRWAALNGKRFTDITIET
                     ESVFSWRGEYLEEIDAGMTELHVVYHVQGQGLTQADICDMADTVARRCPVFATLRRAV
                     LIKEQLELAGHPALRREWNPGVAEAVAVV"
     gene            2133..2825
                     /locus_tag="VIR04_04440"
     CDS             2133..2825
                     /locus_tag="VIR04_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY9460029.1"
                     /translation="MSAINVFCAGAVKSALIEALREFEQHSGHTVQCTFGSTGSLRSK
                     IVAGELADIILMSRSPVEQLAHQGRVLADTIADVGRVGVGVAVRANAALPDVSTPDAL
                     RASLRAARSITFGDPSKGDSSGIHFARVIETLGIKDELSDKIVLAPLGLAVAELIDQG
                     KVELGATQATVILACNGIVLAGLLPEGLQHITTYALGVVSDSGVLPAAHNLADYLAAP
                     AAKACFRAAGFA"
     gene            complement(2913..>3085)
                     /locus_tag="VIR04_04445"
     CDS             complement(2913..>3085)
                     /locus_tag="VIR04_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY9460030.1"
                     /translation="NFFGGDLAKQNTYNLGYTYGMSKRTDLYAVASYAKNASFQRDLK
                     STFVSVGIRHRF"
BASE COUNT          702 a          834 c          891 g          658 t
ORIGIN      
        1 agcatcaacg cccaggtcag ctcggcggtc gtcgatccgt cgccctttcc ggccatcacg
       61 acaatgccgc gctcgctaca tgccggcaaa tcgacatgag cgccgatagc tcctgtctgg
      121 ctcaacatgc gcaacttggg cagtcgatcc agcaactctt tcttgatcgc cgtgcgctga
      181 cgcatcagga cgaccgcatc cgcatcggcc aagcgctccg ccaaagcatc aacgcctttg
      241 accgtgtcgt taaaaattcg aacgttatgc tgcgcaatac ttgagaagca attcaaatga
      301 cgaacacagt cctgatagtc gtccaatatc gtgatattca tggggcgttc cttggctcat
      361 ttgttgattt aataaatagc gggctcagtc tcctgcatgc tcatcataac gccacgaaca
      421 agccgcgtga acgtgtgtca tgcaggggtt cgtgaactgg caaaggtttt cgagatacag
      481 tatgctgcac gcaataaacg catatggttt gtacgccttg gcgggaactt aaaatgcaga
      541 taattagatc cgagacatgc ttgaaaaaaa taacacagac ggctgctgcc atcctgctgg
      601 ccacgggctt gtcgagccct tcgcttgcag acacctggcc agaccagccc atacacttca
      661 ttgtcccgta tacggcaggc ggcggcgccg atggagtcgc ccgtgttgtc ggcaataagc
      721 ttggcaaggc actgggccag ccggttatta tcgaaaatcg cggcggcgcc gcgggcaata
      781 tcggcacgaa tctggccgcc aaggccgatc cgaacggata caccattatg ctgggagcgg
      841 ccgcgctagc catcaatgag accctgtata aaaagctgcc attcgatccg gtcaaagact
      901 tcacgcctgt tacgctgctg gtaaaaacac ccaacatact ggtcgtgaat cccaagttgc
      961 ccgtcaaaac ggtcaaggag ctgattcaac ttgccaggag caagccgggc gtgctgaact
     1021 atgcatccgc gggtaagggt acgactccgc accttgcagc ggaactgttt gattctatgg
     1081 cgggggtcaa gatgacccat attccataca agggttcttc ccccgctgta gtggcgttgg
     1141 tcggcgatga agtagacgtt atgctgtcgc cagcactgac cgttctgcct tttatcaaga
     1201 cgggccggct aagagcactc gccatgactg gcgccacgcg gtcaaccgcc ttccccgata
     1261 ttcccaccat cgctgaatcg ggattgcccg gatatgaagc atcgcagtgg tatgcagtat
     1321 tcgcgccggc tggaaccccg gcggatatcg tggcccgact gaaccgcgaa ctggtcaaga
     1381 tcgtgcactc gcccgacgtg gaaaaagttt tcgcactcga gggctcggaa cccataggcg
     1441 gtacgcccga agaactgggt caatacttca aatctgaaat cgcgcgatgg gcaaaaacga
     1501 tcccgtccgg tgagcggctt aattaacaag aacttgcaga aaggaccatc gtggatgttg
     1561 attatgcagg cctgaaaaaa agtgtcgcga acacgatcgc agaagtaaag agcggaagca
     1621 aagagccgat tcgccggccc aaggcgcggc tacgcgtggt gcgaggactg caaggcgagg
     1681 taacggccgg caagttcaca tatcgtaccg atgccgcgct ggatgcgggc ggttttgcgg
     1741 aacaccccag gcctatggat tatctgctgg gtgcgctggc cagttgccag caaatgtggt
     1801 gtttgcgctg ggcggcgtta aacggcaagc gctttaccga cataaccatc gagacagagt
     1861 cggtattttc atggcgcgga gaatatctgg aagaaatcga tgcgggcatg accgagcttc
     1921 atgtcgtcta tcatgtccag ggccagggac tgacccaagc ggacatctgc gacatggctg
     1981 acacagtggc tcggcgttgc cccgtgttcg ccacgctgcg ccgtgcggtt ctgatcaaag
     2041 aacagttgga attggccggg catccggcgt tgcgtcgcga gtggaaccca ggtgttgcag
     2101 aagccgtcgc cgttgtctga ggaaaaataa tagtgagtgc gattaatgta ttttgcgcgg
     2161 gcgcggtcaa gtccgcgcta atagaggctc tgcgcgagtt tgagcaacat tccggacata
     2221 cggtgcagtg cacgtttgga tccaccggca gcttgcgaag caagattgtt gcgggtgagc
     2281 tcgccgacat catattgatg agccggtcgc cggtcgaaca acttgcgcat caaggccggg
     2341 ttctggcgga tacgatagcc gatgtgggtc gggtgggagt cggggttgcc gtgcgcgcca
     2401 atgccgctct gcccgatgtt tccacacccg atgccttgcg ggcgtctttg cgcgcggcca
     2461 gatccatcac gtttggcgat ccatccaaag gcgattccag cgggattcat tttgccaggg
     2521 tgattgaaac gctaggtatt aaggacgagt tgagtgacaa gatagtcctg gctccgctgg
     2581 gcttggccgt ggcggagctt atcgatcagg gaaaggtcga attgggcgcc acgcaggcca
     2641 ccgttattct tgcctgcaac ggcattgtgc tcgctggttt attacccgaa ggcttgcaac
     2701 acatcaccac atacgcgttg ggcgtggtgt ccgatagcgg cgtcttgccg gccgcccata
     2761 atctggctga ttaccttgcc gcgcccgcag ccaaagcttg cttcagggca gccgggttcg
     2821 cataaggcgg gtggccgttg ctcaaaaaaa acccactccg aagaatgggc ttttcacatg
     2881 tgccggagct cgtgcggttt taccgattag agtcagaacc tgtggcgaat gccgacagag
     2941 acaaaagtcg atttcagatc tctctgaaaa gaagcgttct tggcatacga agcgaccgca
     3001 tacaaatcgg tgcgtttgga catgccgtag gtataaccca agttatacgt gttttgcttc
     3061 gccagatcgc ccccaaagaa attgg
//
LOCUS       DATCXU010000200         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_709164,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000200 DATCXU010000000
VERSION     DATCXU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709164"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VIR04_04450"
     CDS             complement(<1..251)
                     /locus_tag="VIR04_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEY9460031.1"
                     /translation="MRPICIYHGKCADGFTAAWAVKCALGDIEFYPGVYQEPPPEVTG
                     RDVIIVDFSYKRPVLVEMAAVARSILVLDHHKTAAEDLAG"
     gene            complement(494..1180)
                     /locus_tag="VIR04_04455"
     CDS             complement(494..1180)
                     /locus_tag="VIR04_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460032.1"
                     /translation="MRPFFPYYGSKWNIARHYPRPAHGIVIEPFAGGAGYATFYNAPR
                     AHLIDADPIIVGVWSYLLRVTPAEILDLPEMPEAGDGVNNYDLPQEAKWLIGFWLNRG
                     SATPKLTRTAYSARADKGQLNWGPRAKERIASQLEHISGWTVCQGDFASAPDVEATWF
                     VDPPYGDKGKFYRVGFKDFARLAPWCHTRRGPLIVCEGPGATWLPFQPLGNFKTTKGK
                     AMEYVFARAA"
     gene            complement(1196..1483)
                     /locus_tag="VIR04_04460"
     CDS             complement(1196..1483)
                     /locus_tag="VIR04_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460033.1"
                     /translation="MGGESSNSRYACFNRAAPLRPCQKVCVGDTAMKLCCEVIAVKSI
                     GTLLTIRLQGSRPDSADWREMGAHEIQIDDTPRNCRAFHLGRRVTVTIEPR"
     gene            complement(1686..1853)
                     /locus_tag="VIR04_04465"
     CDS             complement(1686..1853)
                     /locus_tag="VIR04_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460034.1"
                     /translation="MPLTRGCCYRILPEDAGGWAEFVLYVGRNRDGEFVFQDANGDLL
                     FHHLSDMEATP"
     gene            complement(1904..1984)
                     /locus_tag="VIR04_04470"
     tRNA            complement(1904..1984)
                     /locus_tag="VIR04_04470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Glu,seq:ctc)
     gene            2196..2495
                     /locus_tag="VIR04_04475"
     CDS             2196..2495
                     /locus_tag="VIR04_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460035.1"
                     /translation="MCENSRQEPAGCRFIYGDPGQSDWHYCQRTIVKGGELGNAAHPP
                     YCAHHTKVCVMPAKPFSYYGGWEPYVKHISNPLGRVRYSIDVLFAAQERGVFLND"
     gene            2488..>2687
                     /locus_tag="VIR04_04480"
     CDS             2488..>2687
                     /locus_tag="VIR04_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460036.1"
                     /translation="MTDLSVLSAKESVQFLEWLDPVGLHNLVALDPAGVEAPDAITTA
                     SRSLMQMFVEGHNGKRNIYFSI"
BASE COUNT          561 a          797 c          770 g          559 t
ORIGIN      
        1 ccggctaagt cttcggccgc tgtcttgtga tgatccagca caaggatgga acgggctacc
       61 gccgccatct ccaccagcac cgggcgcttg taactgaaat ccacgatgat aacgtcgcgg
      121 cccgtcacct ccggcggcgg ctcttgatag acaccaggat aaaactcgat atcgcccagc
      181 gcgcatttga ccgcccatgc agcggtgaag ccatcggcgc atttgccgtg atagatgcag
      241 attggtctca tagttttctc cattggttaa atcgctccta ttccgtggtt acttgcgaaa
      301 aatgcgcgtt gaccgcttcg catatcagct ttgagtttgc ggtggggctc gcggacggat
      361 tgcagaccgc gaccagtgta tcgccagaat aaattctgac attgaggccg tgccgggcaa
      421 ctcgccaagg accgggggac ggcatgggat cgccactatt tttcttctca accgtccgca
      481 ccagacgctc caattatgct gcgcgagcaa agacgtattc catcgccttg cccttggttg
      541 ttttgaaatt gccgagcggc tggaacggga gccaggtcgc gcccggccct tcgcagacga
      601 tcagcgggcc gcgtctggtg tggcaccatg gcgccaagcg cgcgaagtct ttgaagccca
      661 cccggtagaa cttccccttg tcgccataag gtggatcgac aaaccatgtc gcctcaacat
      721 cgggcgcgga ggcaaagtcg ccctggcata cggtccagcc ggaaatatgc tcgagctggg
      781 aggcgatgcg ttccttggcg cgcggtcccc agttcaactg ccccttgtcg gcgcgggcgc
      841 tgtaggccgt gcgggtcagc ttcggggtcg cgctgcctcg gttgagccag aacccgatca
      901 accacttggc ttcctgtggc agatcgtaat tgttcacgcc gtcgccggcc tccggcattt
      961 ccggcagatc caggatttcg gcgggcgtca cgcgcagcag gtaggaccag accccgacaa
     1021 tgatcgggtc ggcgtcgatc aggtgggcgc ggggcgcatt atagaacgtg gcatagccag
     1081 ctccgccagc gaacggctca atgacgatgc cgtgagccgg gcgcgggtaa tgccgcgcga
     1141 tgttccactt ggagccgtaa tacggaaaaa aaggtcgcac gttaaaatcg ctccgttatc
     1201 tcggttcgat ggtgacggtc acgcggcggc caaggtgaaa tgcccggcag ttgcgcggcg
     1261 tgtcgtctat ctgaatttca tgggcgccca tctctcgcca atcagcggag tcggggcgcg
     1321 atccttgaag gcgaatggtc agcagcgtgc cgatggattt caccgctata acttcgcaac
     1381 acagcttcat cgcagtatcc cctacgcaga ctttctggca gggccgtaat ggcgctgcac
     1441 ggttgaaaca ggcataccta gaattgctgc tttctccgcc catggcatgt cactgcaaat
     1501 ggatgagtgt gcgaaattgg cggaaattcg tctgttcatt agagcaaccc caagggcgcc
     1561 aaacccgcgc taaagaaacg cccgcacgat cagccgcccc aggtcgtagg cgccgaccga
     1621 catcagcacg gccatgatcc ccgacgccga catcacgccg gcgaaggtcc aatcgaatcg
     1681 cgcattcatg gcgtggcctc catgtccgaa aggtggtgga acagcaaatc cccgttggcg
     1741 tcctgaaaga cgaattcgcc atcgcgattg cggccgacat aaaggacgaa ttcggcccac
     1801 ccgccggcgt cttcgggcag gatccggtag caacagcctc tcgtaagcgg catgaggcca
     1861 accgcctcaa cgggctgggg aatttccacc tcgctgcagg ccgtggcgct cccagcggaa
     1921 ttcgaatccg cgtttcggcc ttgagagggc agttcactcg ctttcgcaat ctcaagtctg
     1981 attttcatat ggtccctgtc cctttatgct taaatctggt ttcaccaatc gccgctcccc
     2041 gcgtcatccg acgaccaagg ttccggtatg gttagcattg acacaaacgg acaagcggtg
     2101 caagttgccc cgcatcaaat aacacgcgca cagagaaaaa gtttcgagat cgagagccgc
     2161 gcggttcttc aaaagcgaaa aaggcgcgat ctccaatgtg cgaaaactct cgtcaagagc
     2221 ctgcgggctg ccgtttcatc tatggcgatc ctggccaatc agactggcat tattgtcagc
     2281 gaacaatcgt aaaaggcggc gaattgggca acgcggcgca tccgccatac tgcgcccacc
     2341 ataccaaggt ctgcgtgatg ccggcaaagc cgttttccta ttacgggggt tgggaaccct
     2401 acgtgaagca catcagcaac ccgctaggcc gcgtccgtta ttccattgac gtccttttcg
     2461 ctgcgcagga acgcggagtt tttctcaatg actgatctgt ccgttttgtc cgctaaagaa
     2521 tccgtacagt ttttggagtg gctggacccg gtggggttgc acaatctggt ggcgctggac
     2581 cccgcgggtg ttgaggcgcc cgacgctatt acgaccgctt ctcgctcgct gatgcagatg
     2641 tttgtcgagg gccacaatgg caagcgaaac atctatttca gcatcaa
//
LOCUS       DATCXU010000201         3785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_206146,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000201 DATCXU010000000
VERSION     DATCXU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206146"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VIR04_04485"
     CDS             complement(<1..401)
                     /locus_tag="VIR04_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460037.1"
                     /translation="MPNSNLQSDAVGLAFAADDDVPIPYRKRTRDWYLALGYDNPYRW
                     AHYASVPFQPLEKPLSESTLGLITTAAPYQPDKGNQGPGAAYNASAKFYNVYTLDTAH
                     DHDLRISHVAIDRKHTSMEDSGTWFPLPALR"
     gene            483..1730
                     /locus_tag="VIR04_04490"
     CDS             483..1730
                     /locus_tag="VIR04_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9460038.1"
                     /translation="MIVKLLFSVVAAALTAASASAATAPSEIKIGTLYASSGNYAAIS
                     MPVYTGLKFWVDQQNADGGVFVKPYNKKIPIKLVSYDDQSNPATAGTLYNQLVTQDKV
                     DILITDSGSVLTAPAVPVSLNHKMLLIDQTGTGASFFNKGNPYIVLAADPASTVWPKY
                     LANFLIHDGPALGIKRIALIYATNEFTGTQANAVRDYIKKSGAPLEFVYDQGVPTNTT
                     NYSVLINNIKAANPDAVIQMGYPNNDIAFLRNLQSNAVKFKFLFDIYPGLETSHLLQT
                     VGAKGMLYGTTYVTSSDLSYKPEVGLNLEQYKKAWEAKNSGGKVDFGFNAVAGYTTGL
                     IVESTLKTTDSMDQMALRKAVGNLSGKLKTLDGTFEIDETGAQIGEVTPLGQLVSDGK
                     DGVKFTVLYPHDVATGKIVYPAK"
     gene            1772..2647
                     /locus_tag="VIR04_04495"
     CDS             1772..2647
                     /locus_tag="VIR04_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460039.1"
                     /translation="MVEYSLIAGLVFGMYYCLVGLGLNLVFGVMRIVNLAHGDFLMLG
                     AFAAIWLFSGLGVHPLWAIPIATIVFIAVGIPLYYALVPRLLKAKDPEMLSFILFFGL
                     SQIIEALATIVFGPSERSLPGTLMGSEPFHVLGQSLPAAWIVGGVASVLAVGCVYIYL
                     YRTHLGNLTRAVMVDRDEALATGINVNRVSSIAFGVGLALAAVSGIFAPFMLGSITPA
                     MGVDMTIQSFAVIVIGSLGNPMGTVLGGLIYGVSQIMMQTYLSSWAGLLPYVLLILIL
                     LIRPSGLLGRTVRNA"
     gene            2640..3560
                     /locus_tag="VIR04_04500"
     CDS             2640..3560
                     /locus_tag="VIR04_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460040.1"
                     /translation="MHRAIRHSLLTLVPVVAAAALLPLWYSNQLMLFNFIVFLILAQG
                     INIIYGFTGYLPFGFVGFFGAGAYGFALAIMHLHAGPLAAMIMGGGITVLLALVLIPL
                     LRLSGAYFAIANLAAALAIYQVISNPALEDITRGPYGVSLSGVFNPTLSYAIAVGILA
                     ATLGVVIYLRNSHFGLSLQAIKDDPFSASMSGVNVVRARTIAWLVSALVAGLIGSDFA
                     WYISAFYPDTVFSTDYSVFAIVFALFGGVATATGPILGVLFLYGLYNYIGISAPQYFQ
                     LTYGVLIVVIVLFLPTGLVSLLQRKGIDVP"
     gene            3550..>3785
                     /locus_tag="VIR04_04505"
     CDS             3550..>3785
                     /locus_tag="VIR04_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460041.1"
                     /translation="MSLKPQEKAGEPLLVVDKLVKQFGGFRALDGVSFDVRPGEILGL
                     VGPNGSGKTTCINVITGLYSADGGTVRFQGKPISG"
BASE COUNT          826 a         1026 c         1020 g          913 t
ORIGIN      
        1 tcgcgcaacg ccggcagcgg gaaccatgtg ccgctatctt ccatggatgt gtgcttgcgg
       61 tcaatggcaa catgggagat gcgcagatca tggtcatgcg cggtgtcaag tgtatatacg
      121 ttgtagaatt tggcggacgc attgtatgct gcccccggcc cttggttccc tttatccggt
      181 tggtaaggcg ccgccgtcgt gatcaggcca agcgtggact ccgacaatgg tttttcaagc
      241 ggctggaacg gcacgcttgc gtagtgcgcc catcgataag ggttgtcgta acccaacgcc
      301 agataccagt cgcgggtgcg cttccggtac gggataggca catcgtcgtc agccgcaaag
      361 gccaagccga ccgcgtcgga ttgcaaattg gagttcggca taatcctgaa ttgacagtca
      421 acaaaggcac gtattatatt gaatataggg ggtgtcgaac atgcgtattg caggagacat
      481 caatgattgt aaagttactt ttttccgtgg tcgcggcagc tctaacggcg gcgagcgcca
      541 gcgcggcaac ggctccatca gaaataaaaa taggtactct ttatgcctca tcgggcaact
      601 acgcggcgat ttcaatgccg gtttacacgg gtctgaaatt ctgggttgat cagcaaaacg
      661 ctgatggcgg ggtattcgtc aaaccataca ataaaaaaat cccgatcaag ctagtttcct
      721 acgacgacca gagcaacccg gcaacggcgg ggacgctgta caaccaactg gtcacacaag
      781 acaaagtcga tatccttatt acagactcag gatcggtatt gacggctccc gccgtgccag
      841 tatctctcaa tcacaaaatg ctgctgattg atcaaacggg cacaggcgct tcatttttca
      901 acaaaggcaa tccttatatc gtacttgcgg ccgacccggc ctcgacggtc tggcccaaat
      961 atctggccaa tttcctgatc catgatggtc cggcgctagg catcaaacgc atcgcgctca
     1021 tatacgccac caacgaattc accggcacgc aggcaaacgc tgtgcgcgac tacatcaaga
     1081 agtccggagc gccgctcgaa ttcgtttacg accagggcgt tccgaccaac acgacgaact
     1141 attcggtgct gatcaacaac atcaaggcgg caaacccgga tgccgttatt cagatgggct
     1201 accccaacaa tgacatcgcg tttctacgga atttgcaatc caatgcggtg aaattcaaat
     1261 tcctgttcga catttatccc gggctggaaa ccagccattt gcttcaaacg gtcggcgcca
     1321 agggaatgct gtacggcaca acctacgtca cttcgtcaga ccttagctac aaaccagaag
     1381 tcggacttaa tttggaacaa tataaaaaag cctgggaggc gaaaaatagc ggaggcaagg
     1441 tcgactttgg ctttaacgcg gtggctggct acaccacagg ccttatcgtc gaaagtacgc
     1501 taaaaacgac agacagcatg gaccagatgg cgctccgtaa agcagttggc aatctgtcgg
     1561 gcaagcttaa aaccctggac ggcaccttcg aaattgacga aacaggcgct caaatcggcg
     1621 aagtgactcc gttgggacaa ctcgtttcag acggtaaaga cggcgtcaaa ttcaccgtgc
     1681 tgtatccgca tgatgtcgca accggcaaaa ttgtgtatcc cgccaaatga gcgtatcaag
     1741 catcagccag caaccaatga gagactgctg agtggttgaa tactctctga tcgccggctt
     1801 ggtgttcggc atgtattact gcctggttgg cctgggcctg aatctggtgt ttggcgtcat
     1861 gcgcattgtt aatcttgcgc atggcgattt ccttatgctg ggcgcctttg ccgccatttg
     1921 gttattcagc ggactgggcg tgcacccgtt atgggccata cccatcgcca cgatcgtatt
     1981 catcgcagtg ggtattccgc tgtactacgc actggtgccg cgactgttga aggcaaaaga
     2041 cccggagatg ctgtccttca tactattctt cggcctgagc cagattattg aagcgttggc
     2101 aaccatcgtg ttcgggccca gcgaacgttc actgccaggc acactgatgg gatccgagcc
     2161 tttccatgtg ctgggtcaaa gtctgccagc cgcatggatc gtcggcggag tggccagtgt
     2221 tctcgcggtc ggctgcgttt atatttacct gtatcgcacc catctgggca accttacgcg
     2281 cgccgtcatg gttgaccgcg acgaagcact ggcgacaggc atcaatgtca atcgcgtttc
     2341 gtcgattgcc tttggcgtcg gactggcgct ggcggcggtg tcgggcattt tcgcgccatt
     2401 catgcttggc agcattacgc cggcaatggg cgtcgacatg actatccagt ctttcgcggt
     2461 catcgtcatc ggctcactgg gcaacccaat gggaacggtc ctaggcggcc ttatatatgg
     2521 cgtttctcaa ataatgatgc aaacctattt aagctcatgg gcaggcctgc ttccttatgt
     2581 ccttctgata ctcattctac tgattcgtcc cagcggcctg ttgggcagga cggtgcgcaa
     2641 tgcatagggc aattcgccac agcctattga cgctcgttcc ggttgtggcg gccgcggcgc
     2701 tgttgccgct gtggtattcg aatcaattaa tgctatttaa ctttattgtg ttcctgatcc
     2761 tggctcaggg aatcaatatc atctatgggt tcaccgggta tttgcccttc ggcttcgtcg
     2821 gattcttcgg cgccggcgcg tacgggttcg cactggccat tatgcatttg catgcgggcc
     2881 cgctggccgc aatgataatg ggcggtggca tcacggtatt gctggcgctg gttctaatac
     2941 ctttgttgcg gctatcgggc gcctacttcg ccatcgccaa tttagcggcc gccttggcaa
     3001 tataccaagt gatttccaac ccggctctcg aagacatcac ccggggaccc tatggcgtca
     3061 gcttgtcggg ggtgttcaat ccgacgttaa gctacgccat tgcggtgggc atactggccg
     3121 caacgctggg ggtcgtcatc tacttacgca actcacattt cggcttgtcg ctacaggcca
     3181 tcaaggacga tccgttcagc gcctcgatgt cgggcgttaa tgtggtgcgc gcgcggacca
     3241 tcgcatggct cgtttccgcg ctggttgccg gactgattgg cagcgatttc gcctggtata
     3301 tatcggcatt ctatcccgat accgtgttca gcacggacta cagcgtattc gccattgttt
     3361 tcgccctgtt tggcggcgtt gccactgcaa ccggccctat tctgggcgtg ttatttctgt
     3421 atggtctata taactacatt ggcatctcag ctccgcagta ttttcaactg acttatggcg
     3481 tgttaattgt cgtcatcgta ctgttcctgc ccaccggact ggtctcgctg cttcaacgca
     3541 aaggaatcga tgtcccttaa gccgcaagaa aaagcgggcg aaccgctact ggtcgttgat
     3601 aaactggtca agcaatttgg cggatttcgc gcgcttgacg gagtcagttt tgacgtccgc
     3661 ccaggcgaaa tactgggcct tgtcgggcca aacggctctg gaaagacgac ctgcatcaat
     3721 gtcataaccg ggctttacag cgccgacggc ggcaccgtgc gctttcaagg caaacctatc
     3781 agcgg
//
LOCUS       DATCXU010000202         5675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_894718,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000202 DATCXU010000000
VERSION     DATCXU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894718"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VIR04_04510"
     CDS             <1..591
                     /locus_tag="VIR04_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9460042.1"
                     /translation="SALLPMLALGIPGSATAAVLLGGLMIWGLEPGPLLFVEHKDFVW
                     GLIASMYLGNIVGLIFVMSTVPLFAAVLRIRFSTIAPIIIVLCAIGAFSVHNAVLDIW
                     FMVLFGIIGYVLKKLEYPLAPLVLALVLGDRMEDAFRQSMLGPGTGLHVFWSNWLVGT
                     VTTLALLMLVWPLIPFVWRLVRRVFGGNRKLAEKAS"
     gene            629..1408
                     /locus_tag="VIR04_04515"
     CDS             629..1408
                     /locus_tag="VIR04_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY9460043.1"
                     /translation="MSDLVLLERDGSIATVVLNRPAKLNALTRPMWQALGDAINALSA
                     DNDVRCIVLRGAGEKSFSPGNDIAEFQTERADKAQAIAYGKVMHATIGALARCRHPLV
                     AQIHGICVGGGLEIAAMCDIRICGESSRFGAPIKNLGLVMAYQEMASLVRLAGPDVAL
                     EILLEGRIFDATEAKSKGLVTRIVSDDQVAAEAMASARRIADGAPLVAQWHKKFARRL
                     ADPRPISDAENDECFDCFDTEDFRIGYAAFLAKQKPAFLGK"
     gene            1421..2656
                     /locus_tag="VIR04_04520"
     CDS             1421..2656
                     /locus_tag="VIR04_04520"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9460044.1"
                     /translation="MTVGTAHTGPLVGMRVLELAQIMAGPTCGMMLADMGADVVKVEK
                     LPGGDDARGYREPRVNGVSAPFMILNRNKRGIALNLKHEQGRDVLLRMVQDADVLTEN
                     YRRGTLEKLGLGYDVLAKVNPGLIYCAVSGYGRDGPYGAKPGFDLIAQGFAGLMSITG
                     EPGRPPVKTGNSIADINAGILAVVGITAAYAHKLKTGRGQVVDTSLMEAALQQTYWQA
                     ASYFATGESVGPTGSAHLLTAPYQAFRTSDGWVNIGGANQANWERIADVLGHPEWRTD
                     SRFATNTARMENLAALTAAMDAVLKDHNTAHWIQAFDSAGVPVGPVHTIGQALSHPQT
                     LARNMVVDLVHPQAGPTKALGCPIHFSETPTQITRPAPMLGQHSRELLREYGYDDSQI
                     DGFVANGVIQDSPANIAMA"
     gene            complement(2671..3336)
                     /locus_tag="VIR04_04525"
     CDS             complement(2671..3336)
                     /locus_tag="VIR04_04525"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY9460045.1"
                     /translation="MDADFWLKRWREGHIGFHQERVTPLLQKYWPSLLLPSGSRVLAP
                     LCGKSLDMIWLAEQGHRVLGVELSSLAVDQFFTENDLHPKVHDSNLGRHHIAGNIEII
                     CGDIFALDPATLAQCTGVYDRAALVALPRDMRKRYAQHVYGSLSKHYRGLLLTLDYRQ
                     EQMDGPPFAVHEAEVTTLLGQYSDVKIIDRRDILAKEPKFAERGLTRLDAVVYRLGGK
                     TGM"
     gene            complement(3357..3962)
                     /locus_tag="VIR04_04530"
     CDS             complement(3357..3962)
                     /locus_tag="VIR04_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY9460046.1"
                     /translation="MKIYFSPASPYVRKCMVVAHELGLEAQIEKLPSAANPVNRDASI
                     VATNPLGKVPTFFTDDGQVLYDSRVVCEYLNELGKGNLFPKEGHLKWQILTEQSLADG
                     ILDAALLVRYENAARPQEFRWSAWTEGQMDKIKSAIAQFEKTINGRGNVVDISRISLA
                     CALGYLDFRFADYDWRAEHRALSAWYAEFSKRASVSCTAPK"
     gene            4067..4273
                     /locus_tag="VIR04_04535"
     CDS             4067..4273
                     /locus_tag="VIR04_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460047.1"
                     /translation="MVVFTILTDIIAAVGMARFAVLRQINIMPVKTAAFRSKAQQFYR
                     PSPEAPPCAVCRRKVQLAFLTVVG"
     gene            4353..5036
                     /locus_tag="VIR04_04540"
     CDS             4353..5036
                     /locus_tag="VIR04_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460048.1"
                     /translation="MTEFWPSLLEGAWLSIKMSTLSIVLGFLLGVALAIAKTEGGWFL
                     KGATTAFVDVTRNTPLIVQAFWLFFGLAALQIRVPAFYAAIVALTINTSGYTCEIVRA
                     GMEAVHKGQKEAAACLSFTKAQILRYIVLPQAVEKMYPALVSQFVLMMLASSIMSQIS
                     AEELTAVGYQIQSETFRGFEVFIVIAIIYVFLSWLLRVSMNACYDFAFPKAKNIEKEG
                     RATREDLQS"
     gene            5033..>5675
                     /locus_tag="VIR04_04545"
     CDS             5033..>5675
                     /locus_tag="VIR04_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460049.1"
                     /translation="MIFGVSYSQWLFVLHGAGWTIVLSLLGFVGGTIAGLPLAISRAS
                     KHKLISGTTGLYVKLMQGIPLPVMMFIVYFGISIAGYELPALVAAGLAMTIYSSAYLG
                     EIWRGCIESVPLTQWEASHSLAFNGLQTLAYIVLPQAFRVAIPPTVGFLVQIIKNSSY
                     AVVIGFFDLTYSARVVNNSTFKPFAVFTAAALIYFILCYPLSLWSYRLEKKLKD"
BASE COUNT         1145 a         1469 c         1658 g         1403 t
ORIGIN      
        1 tccgcgctgc tgcccatgct ggcattgggc attcccggtt cggcgacggc tgcggtattg
       61 ctgggtggtt tgatgatttg ggggctcgag ccgggtcccc tgttatttgt cgaacacaag
      121 gattttgtct ggggtttgat cgccagcatg tatttgggca atatcgtggg attgattttt
      181 gtgatgtcca cggtgccgct gttcgctgcg gttttacgca ttcgtttttc cactattgcg
      241 cccatcatca tcgtattgtg cgcaattggc gctttttctg tgcacaacgc tgtgcttgac
      301 atctggttca tggtgttgtt tggcatcatc ggctatgttt tgaagaagct ggaatatcct
      361 ttggcgccat tggtgcttgc tctggtgctg ggcgaccgga tggaagacgc attcagacag
      421 tccatgttgg ggccaggcac cggcttgcat gttttctggt ctaactggct ggtgggcacc
      481 gttaccactt tggctttgct tatgctggtt tggccgctga ttcctttcgt gtggcgcctc
      541 gtgcgcaggg tttttggcgg caaccgcaaa cttgcggaaa aagcatcgtg accgccgttt
      601 ccttcatggc atattctgga gtggctcaat gtccgattta gtcctgctgg agcgcgacgg
      661 atcaattgcc accgtcgtgc ttaatcgccc cgcgaagctg aacgcgctga ccaggccaat
      721 gtggcaggcg ctgggcgacg ccataaatgc actgtcggcc gataacgacg tgcgttgcat
      781 tgtgctgcgc ggtgcgggtg aaaaatcttt ttcgcccggc aacgatattg ccgaatttca
      841 gaccgaacgc gccgacaaag cgcaggcgat agcatatggc aaggtcatgc atgccactat
      901 tggcgccctg gcccgttgcc ggcacccgct ggtcgctcag attcacggca tttgcgttgg
      961 tggcggcctg gaaattgcgg ccatgtgcga tattcgcata tgtggcgaat ccagccgctt
     1021 tggcgcaccc ataaaaaatc tgggcctggt gatggcttat caggaaatgg cttcgttagt
     1081 ccggttggca ggccccgatg tggctttgga aattttgctc gaagggcgaa ttttcgacgc
     1141 gaccgaagcc aagtccaaag gactggtcac gcgtattgtg tccgatgatc aggtggctgc
     1201 cgaggcaatg gccagcgcac ggcgcattgc cgatggcgcg ccattggtgg cgcaatggca
     1261 caagaagttt gcgcgccgcc tggccgaccc gcgaccaatt tccgacgccg agaacgatga
     1321 gtgctttgat tgcttcgaca cggaagattt tcgtatcggg tatgcggcat ttctagccaa
     1381 acaaaagcct gcttttcttg gcaaataaca ggattttaaa atgacggttg gtacagctca
     1441 tacgggccca ttggtgggca tgcgcgtatt ggaattggcg cagattatgg ccggacccac
     1501 atgcggcatg atgttggccg atatgggcgc cgatgtcgtc aaggtggaaa agctgcctgg
     1561 cggcgacgat gcgcgcggct atcgcgagcc ccgtgtaaac ggcgtttcgg cgccgttcat
     1621 gatactgaat cgcaataagc gcggcattgc gctgaacttg aagcacgagc aaggccgcga
     1681 cgttttgctg cgcatggtgc aagacgccga tgtattgacc gaaaattacc gccgcggcac
     1741 actggagaag ctgggcctgg gctacgatgt gttggcgaaa gtcaacccgg ggctgattta
     1801 ctgcgctgtg tcgggttacg ggcgcgacgg cccctacggc gcgaagccgg gttttgatct
     1861 gattgcccag gggtttgcgg gcttgatgtc cattacaggc gagcccggac ggccgcccgt
     1921 gaagaccggc aattccattg cagatattaa tgccggcatt ttggcggtag tgggcattac
     1981 ggcagcctac gcgcacaagc ttaaaaccgg ccgtggccag gtggtggaca cgtctctgat
     2041 ggaggcggcg ttgcagcaaa cttattggca ggcagcaagc tactttgcta ccggcgagtc
     2101 ggtcgggccg accggatcgg cgcatttgct gacggctccc tatcaggcgt ttcgcaccag
     2161 cgatggctgg gtcaacatcg gcggagcgaa tcaggcgaac tgggaacgca tcgccgacgt
     2221 gcttggccat cctgaatggc gcaccgattc acgttttgca acgaatacgg cgcggatgga
     2281 aaatttggcg gctttgactg ccgccatgga tgcggtgtta aaagaccaca ataccgctca
     2341 ctggatccag gcgttcgatt cggcgggagt gcccgtcggg cctgtgcata cgatcgggca
     2401 agcgctttcg catccgcaga cgctggcgcg aaatatggtg gttgatctgg tgcacccgca
     2461 ggccggcccc accaaggcgc tggggtgtcc gatccatttt tccgagacgc ccacacagat
     2521 tacccgccca gccccaatgc tggggcaaca ttcgcgcgag ctgttgcgcg aatatgggta
     2581 tgacgatagc caaatcgatg gctttgtcgc caatggcgtc atccaggatt cgcccgcaaa
     2641 tatcgcgatg gcatagggcg ttggcgcgcg ttacatgcca gttttgccgc caagccggta
     2701 aacgaccgca tcgagccggg tcaaaccccg ttcggcaaat ttcggctctt tagccaggat
     2761 gtcgcgccgg tcaatgatct tgacgtcgga atactggccc aatagcgtcg ttacttcggc
     2821 ctcatgaacc gcgaacggcg gaccgtccat ctgttcctgg cgatagtcca gtgtaagcag
     2881 cagaccgcga tagtgttttg agagcgatcc gtaaacatgt tgtgcgtaac gtttgcgcat
     2941 gtcgcgaggc agggccacaa gagcggcgcg gtcgtagact cctgtacatt gcgccagtgt
     3001 ggccgggtcc agcgcaaaga tgtcgccgca aataatttcg atatttcccg caatgtggtg
     3061 ccgacccaga tttgaatcgt gaaccttcgg gtgcaaatcg ttctctgtaa aaaactgatc
     3121 cacggccaaa gaggacagtt cgacccccaa tacgcgatgg ccttgctctg ccaaccagat
     3181 catatccagt gattttccgc aaagcggcgc caatacacgg ctgcccgatg gcagcaacag
     3241 ggacggccag tatttctgca gcagcggcgt cactctttct tggtgaaagc cgatgtgccc
     3301 ttcgcgccag cgtttcagcc agaaatcggc atccatggct tttccctaat gtattgtcac
     3361 tttggcgctg tgcatgacac cgatgcccgt ttgctgaatt cggcgtacca ggccgacaaa
     3421 gcgcgatgtt cggcgcgcca atcgtagtcg gcaaatctga aatccaggta tcccaaagcg
     3481 caagccaaac tgatcctgct gatgtcgaca acgttgcccc ggccgtttat cgttttttcg
     3541 aattgcgcta tggcggattt gattttgtcc atttgcccct ctgtccaagc gctccagcga
     3601 aactcttgcg ggcgggccgc attttcgtag cgtaccagca gggccgcatc caggatgccg
     3661 tcggccagcg attgttcagt caggatttgc catttaagat gcccctcttt ggggaacaga
     3721 ttgcctttgc ccagttcatt aaggtattcg cagacgacgc ggctgtcgta tagaacttgt
     3781 ccgtcatcgg tgaaaaatgt cggcaccttg cccaaagggt ttgtggccac gatgcttgca
     3841 tcgcgattga ccggatttgc ggcgctgggc agtttctcga tttgggcttc cagacccagt
     3901 tcatgagcta caaccataca cttgcgcaca tagggcgagg cgggcgaaaa ataaattttc
     3961 attgttatcc ttcgaattga ataaaaaagt tttcctattc tatttcttaa ttggccgcag
     4021 aggcgaaaat gcgcgcagcc attgcaatct tgcaggcggc gcacacgtgg tcgtatttac
     4081 catattgact gatataattg cggcggttgg catggcccgg tttgctgtgc tgcgccagat
     4141 caacatcatg ccggtcaaga ccgcggcatt ccgaagcaaa gcccaacagt tttatcgacc
     4201 atccccggaa gcaccgccgt gcgcggtgtg ccggcgcaaa gtgcaactgg cgtttttgac
     4261 agtggttggc tgaatgtcaa ttttgaagtc gttgagcggg acatacgtga agagtaaata
     4321 aaaatatgaa ttatggtttc gactttagtt tcgtgaccga gttttggccg agtctgctgg
     4381 agggggcctg gctcagcata aaaatgtcga cactttccat agtgctgggc tttttgctcg
     4441 gggttgcgtt ggccattgcc aagaccgaag gcggatggtt cctgaaaggc gccaccacag
     4501 cgtttgtgga tgtcacccgc aatacgccgc tgatcgtgca ggcgttctgg cttttttttg
     4561 gccttgcggc tcttcaaata agggttcccg cgttttacgc ggccattgtc gcgctgacca
     4621 tcaatacctc cggctatacg tgcgaaattg tccgggcggg aatggaagcc gttcacaagg
     4681 ggcaaaagga agcggccgcg tgcctgagtt ttaccaaggc gcagatattg cgctacatcg
     4741 ttttgcctca ggccgtagaa aaaatgtacc cagcgctggt cagccagttc gtactgatga
     4801 tgttggccag ctcgatcatg tcgcaaatat ctgccgagga gctgaccgcg gtgggttatc
     4861 aaattcaaag cgagacgttt cgcggatttg aagtattcat cgtcattgcg atcatctatg
     4921 tatttctttc atggctattg cgggtgtcga tgaacgcctg ttatgacttc gcgtttccca
     4981 aagcgaagaa cattgaaaaa gagggcaggg caacgcgaga ggaccttcaa tcatgatatt
     5041 cggagtctcc tatagccagt ggcttttcgt cttgcatggg gctggctgga cgattgtttt
     5101 atcattgctg ggttttgttg gcggcacaat tgcgggcttg cctttggcga tttccagggc
     5161 gtcaaagcat aaattaatat cgggaacaac cgggctttat gtcaagctga tgcagggtat
     5221 tccgctgccc gtcatgatgt ttatcgtgta ttttggcatc agcattgcgg gctacgagtt
     5281 gccggctttg gtggcggcag ggttggcaat gactatttat tccagcgcct atctgggcga
     5341 aatatggcgc ggatgcatcg agtcggttcc gctcacgcag tgggaggcct cgcacagcct
     5401 ggcgttcaat ggcctgcaaa ccctggcata cattgttttg ccccaggcat tcagggtggc
     5461 gattcctccc acggtcgggt ttctggtcca gatcatcaag aactcctcct acgcggtggt
     5521 gattggcttc ttcgatctga cctattcggc cagggtcgtc aacaactcca ctttcaagcc
     5581 ttttgcggtg ttcacggcgg cggcgctcat ttatttcata ctgtgctatc cgctttcctt
     5641 gtggtcgtat cgcttggaaa aaaagctgaa ggata
//
LOCUS       DATCXU010000203         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_810153,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000203 DATCXU010000000
VERSION     DATCXU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810153"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIR04_04550"
     CDS             complement(<1..219)
                     /locus_tag="VIR04_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927883.1"
                     /note="catalyzes the decarboxylation of
                     3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate decarboxylase"
                     /protein_id="HEY9460050.1"
                     /translation="MKYRDLRDFIAQLEKAGEFKRLSHPVSTDLEITEISDRVLRAGG
                     PALLFEHARHKGASAGMPILTNLFGTPRR"
     gene            303..1316
                     /locus_tag="VIR04_04555"
     CDS             303..1316
                     /locus_tag="VIR04_04555"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY9460051.1"
                     /translation="MAESVSENRFLYYARGVEHSLGEFVHVSAIYLGIAVLVTLVLSV
                     TVPSLREQSRQMHTALLAALRPDSLSDDMINSAGQSQDNFFSSVAESALTPSDNLASA
                     SSGPSAAATPANPATSAAPVAPVATVAAGPPATNGKPLPKKGDVKPDLSTAADFSNAL
                     STATGRLSIPGVSGVQAQALRSYIARKYKIAHNVAGVLIKAAFVVGKEQHLDPQLLLA
                     VIAVESRYNPFAESHIGAQGLMQVMTEVHKDKFKGFAEGTIAALNPVANMRVGSQILY
                     DCIKRRGSVKAGLACYVGAVGPADNGYGAKVLAERRRIALASGIPIGPDAPVTKADSA
                     SSG"
     gene            complement(1308..2465)
                     /locus_tag="VIR04_04560"
     CDS             complement(1308..2465)
                     /locus_tag="VIR04_04560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY9460052.1"
                     /translation="MPCLAKRTEQIMPFYAVEIFKQASILNAQGRDIVSLGIGEPDFT
                     APAPVIATLNRAAKAGLSGYSPPAGLPLLREAIAHFYETHFSARVEPSRIVVTAGASS
                     ALLMVSMALISPGDEILLPDPSYPANQNFITAAGGTARLIPCPAEQRFQLSAADVRAH
                     WGPATRGVLIASPSNPTGTTIARAELEALITEVRRHDGFIIMDEIYLGLYYDEAPQSA
                     LTLDDNVVVVNSFSKYFHMTGWRLGWLIAPSNMVPAIERLAASLAICAPTLAQHAALT
                     CFEPDVMRIYENRRLSFKQRRDYLLPELERLGLRVPVAPDGAFYIYADISEHSPDSAD
                     FSHRLLHEAGVAAVPGCDFGPAHAKRMLRLSYTTGLDRLHEAVTRIEHFLA"
     gene            2571..3494
                     /locus_tag="VIR04_04565"
     CDS             2571..3494
                     /locus_tag="VIR04_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460053.1"
                     /translation="MESLSRRAFLMGRRAAPTPWESFCQRMRRTVMGSLFDFGLVDGV
                     GSARLVPKNTEDVQHARALCAEYNVILALDGVPQASQLGNQAVLWVEPGIDMAVCQRL
                     EEGSTQWYVQPGCLLGQLEVAGLHQFADLPCHITVAAWLADRTLCDWPSGETYKSGLM
                     HASVLLADGTRANLGPFGENNQKPLDSPSLRRSVSALYELANHADARACRELTSWPAR
                     YRLDALLPCAGQTVNLAHLLLGHGGDLAWVEWLVINEQTTAEPYALAEPGYLARKRAE
                     DKSWLAPVDLDTRVKALFDPSGAFPHPGQDL"
     gene            3598..>4160
                     /locus_tag="VIR04_04570"
     CDS             3598..>4160
                     /locus_tag="VIR04_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY9460054.1"
                     /translation="MEDNFRKAALEYHEHGRPGKIAVTPTKQLSNQRDLGLAYTPGVA
                     AACEEIVSDAKLAYRYTARGNLVGVITNGTAVLGLGNIGALAAKPVMEGKAVLFKKFA
                     DIDVFDIEINETDPDKLIEIIAGLEPTFGGINLEDIKAPECFIVERKLRKRMNIPVFH
                     DDQHGTAICVSSALISGLKVVGKKIDEV"
BASE COUNT          871 a         1161 c         1212 g          916 t
ORIGIN      
        1 tcgccggggg gtgccgaaca agttggtcaa aatgggcatg ccggcgcttg cgcccttgtg
       61 ccgcgcatgt tcgaataaca aagcgggacc gcctgcgcgt agcacccgat cgctgatttc
      121 ggttatttcc aggtcagtcg atacgggatg cgacaagcgc ttgaattcgc ccgctttttc
      181 cagttgcgct ataaagtctc taaggtcacg gtatttcact aaaatctcgc ggatcaggtt
      241 acatttgggc gtcgagctaa ggttaacatc gacttattgt cattttatga gctaaggaag
      301 gaatggccga aagcgtttcg gaaaatcgat tcttgtatta cgcgcgcggc gttgagcact
      361 cgctgggaga gtttgtccat gtttcagcga tttatctggg cattgctgtg ctggtaacgc
      421 tagtgctaag cgtcacggtg ccatcgctgc gcgaacagtc gcgccaaatg cacacagcgt
      481 tgctggcggc cctgcgcccc gattcgctgt ctgacgatat gattaattcc gccggccaaa
      541 gccaggacaa ttttttttcc tccgtggccg aatccgccct gacgccttcc gataatttgg
      601 cttccgcctc ctcgggaccg tctgcggccg ctacccccgc taaccccgcc acatcagccg
      661 caccagtggc gccggtggcg accgtggcgg ccgggccgcc ggcgacgaat ggaaagccct
      721 tgcccaaaaa gggcgacgtc aagcccgatc tgtccacagc cgccgatttc agcaatgctc
      781 tgtccaccgc caccgggcgt ttgtcgatac ccggtgtttc cggggtgcag gcccaggcat
      841 tgcgcagcta cattgcacgt aaatataaaa tagcccacaa cgtggcaggc gtgctgatca
      901 aggcggcttt cgtggtcggg aaagaacaac acctggatcc gcaactgctg cttgcggtca
      961 ttgcagtcga atcgcgctac aacccgttcg ccgagagcca tataggcgct cagggtttaa
     1021 tgcaagtgat gacggaagtc cataaagaca agttcaaggg cttcgcagaa ggcacgatcg
     1081 ccgcgctcaa tcccgttgcc aatatgcggg tgggttcgca aattttatat gactgcatca
     1141 agcgtcgcgg ttcggtgaaa gcggggctgg catgctacgt tggcgcggtc ggtcctgcgg
     1201 ataacgggta cggagcaaag gttttggccg agaggcggcg cattgcactg gcttcgggca
     1261 tcccgatcgg tccggacgcc cccgtgacca aggcggattc ggccagctca ggctaagaag
     1321 tgttcgattc gcgttacggc ttcgtgcaaa cgatccagcc ccgtagtata tgagagccgc
     1381 agcatgcgct tggcgtgcgc tgggccgaag tcgcacccgg gcacggcggc cacgcccgct
     1441 tcgtgcagca agcggtgtga aaaatcggcg ctgtccggac tgtgctcgct aatgtccgcg
     1501 tagatataga aagcgccgtc cggcgcaacc ggcacacgca aacccagccg ttccaattcg
     1561 ggcaagagat aatcgcgccg ctgcttgaac gatagacgcc ggttttcata gatacgcatt
     1621 acgtcgggtt cgaagcacgt caacgccgcg tgctgggcca acgtgggcgc gcagatggcc
     1681 aagctggccg ccagcctttc aatggcaggc accatgttcg atggtgcgat cagccagccc
     1741 aggcgccagc cggtcatgtg aaaatacttc gagaaactat tcacaacgac cacattgtca
     1801 tcaagcgtca aagcgctttg aggggcttcg tcgtaataca gccccagata aatctcatcc
     1861 attattatga aaccatcgtg tcgccggacc tctgtgatca gggcttccag ttcagcccgt
     1921 gcgatggtcg tgccggttgg attgctgggc gatgcgatca gcacgccgcg cgtggccgga
     1981 ccccagtggg cgcgcacgtc ggcggcgctc aactggaagc gctgctctgc cgggcaggga
     2041 ataagccgtg cagtgccgcc cgcggcagtg ataaagtttt ggtttgccgg atacgaggga
     2101 tccggcagca ggatttcgtc gccgggactg atcaacgcca tactgaccat caacaacgcg
     2161 cttgacgcgc cggctgtcac cacgatccgg cttggttcga cgcgggctga aaaatgtgtt
     2221 tcgtagaaat gcgcgatggc ctcacgcagc aatggaaggc ccgccggtgg gctgtatccg
     2281 cttaagcccg ctttggcggc gcgattaagt gtagcaatga ctggcgccgg cgccgtgaaa
     2341 tcaggctcgc caatccccag gctgacaatg tctcggcctt gcgcattaag aatgctggct
     2401 tgtttgaata tttccacagc gtaaaatggc atgatttgtt cggtacgctt ggcaagacaa
     2461 ggcattggtt cgctttctcg ggcaaaaggt ctaattgtaa agcgagtcgc ccttggcggt
     2521 gaagccattt aaaaccggta taaaacagcc ggcgcaaaag gaatcaatat atggagtctt
     2581 tgtctcgccg cgcttttctg atgggtcgcc gcgcggcgcc cacgccgtgg gaaagcttct
     2641 gccagcgaat gcgccgcacg gtcatgggca gcttgtttga ttttggtctg gttgacggtg
     2701 tcggcagcgc gcgtctggtc cccaaaaaca ctgaagatgt ccagcatgcc cgagcccttt
     2761 gcgctgaata caacgttatc ctggcgcttg atggcgttcc ccaagcgtcg caattgggca
     2821 atcaggcagt tctttgggtc gagcccggga tcgatatggc tgtgtgccag cgcctggaag
     2881 aaggcagcac tcagtggtac gtgcaaccgg gatgcctgtt gggccagctt gaggttgccg
     2941 gtttgcacca gtttgccgat ttgccctgtc atataacagt ggccgcctgg ctggctgatc
     3001 gcacgctatg cgactggcca agcggcgaaa catataaatc aggactgatg catgcctctg
     3061 ttttattggc agacggtacg cgcgccaatc tgggcccgtt tggcgagaac aaccagaagc
     3121 ccctcgatag tccttcgctg cggcgatcag tctcggcttt gtacgaactg gcaaatcatg
     3181 ccgatgcccg ggcctgtcgt gaactgacct cctggccggc gcgttatcgc cttgacgctc
     3241 tcctgccttg cgccgggcaa acggtcaatt tggcgcatct tcttctaggc cacggaggcg
     3301 atctggcgtg ggtcgagtgg ctggtcatca acgagcaaac cacagcggag ccatatgcct
     3361 tggctgaacc cggatatctt gcccgcaagc gcgccgaaga caaatcgtgg ttggctcccg
     3421 tcgatctaga tacccgggtc aaggccttgt ttgatccgtc aggcgccttt ccgcatccgg
     3481 ggcaggattt gtaacgttcc tctatggtcc gtatctgtgt tcgcgggctc atttctaaac
     3541 ttccgataca ggggctagaa tgacagccta tttcacttca aaacattttg agtcattatg
     3601 gaagacaatt ttcgtaaagc ggcgcttgaa tatcatgaac atgggcgtcc cggcaaaatc
     3661 gctgtcaccc cgaccaagca gctttccaat cagcgcgatc ttggcctggc ttatacgccg
     3721 ggggtagccg ccgcttgcga agaaatcgtc tccgatgcca aactggctta ccgctacact
     3781 gcgcgcggca atcttgtcgg cgtcattacc aacgggacag ccgttctggg tttgggcaat
     3841 ataggtgcgc tggcggccaa gcccgtcatg gaaggcaaag ccgtattgtt caagaagttt
     3901 gccgacatcg atgttttcga cattgagatc aatgaaaccg atcctgacaa gctgatcgaa
     3961 atcatcgccg gcctggagcc cacttttggc ggcatcaatc ttgaagatat aaaggcgccc
     4021 gagtgtttca ttgtcgagcg caaattgcgc aagcgcatga acattccagt ttttcacgac
     4081 gatcagcacg gcacggcaat ttgcgtttca tcggccctga tcagcggttt gaaagtggtt
     4141 ggaaagaaga tagacgaagt
//
LOCUS       DATCXU010000204         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_60029,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000204 DATCXU010000000
VERSION     DATCXU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60029"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..626
                     /locus_tag="VIR04_04575"
     CDS             48..626
                     /locus_tag="VIR04_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460055.1"
                     /translation="MGWVFPACAQDTVNPQGPPNAFVQAVGNNALNAVRGDAAAKSGD
                     LRRINELVNQYLLPYVNFQKTTQLAAGQYWRSATAQQKKDLAEAFKGTLIRTYGGALN
                     DVDKIAGLNILPFRGDASANDVVVRSTFSQRNGPSIGVDYRLEKSSDGWKIYDLNVEG
                     IWLIQNYRNQFSQQIAQNGIDGLISALNQKNN"
     gene            complement(711..786)
                     /locus_tag="VIR04_04580"
     tRNA            complement(711..786)
                     /locus_tag="VIR04_04580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Gly,seq:gcc)
     gene            974..1996
                     /gene="murB"
                     /locus_tag="VIR04_04585"
     CDS             974..1996
                     /gene="murB"
                     /locus_tag="VIR04_04585"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY9460056.1"
                     /translation="MVLSATTQDLTSFNTLQLHSRTRALVRYRSPAELADLSGLANQY
                     RHVFVLGGGSNVVLAEHLRCLVIKVESRGVRILQQDDHELIIEAQAGEVWPDFVRLCV
                     ARGWDGLENLALIPGTAGAAPVQNIGAYGVELEQRFHSLSVWDLPNRKMLEMGRHDCK
                     FSYRDSLFKHAEPGRWLITAVRFRLPKPWKPVLEYSDLKRHPVLMAPNQAVTATQIFD
                     AVCEIRRAKLPDPAHIGNAGSFFKNPIVTVARYNAIKALYPGLVAYPQDGGLVKLAAG
                     WLIDQAGWKGRRMGKVGVHDRQALVLVNYGGANADDIALLARAVRTDVATRFGVQLEQ
                     EPIRVA"
     gene            complement(2045..>2208)
                     /locus_tag="VIR04_04590"
     CDS             complement(2045..>2208)
                     /locus_tag="VIR04_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY9460057.1"
                     /translation="GDHTVSFCGTGERIEISHRSSSRSTYAQGSLRAARYLSRKQTGL
                     FDMQDVLAI"
BASE COUNT          476 a          550 c          657 g          525 t
ORIGIN      
        1 tccggcgtat gtttgccgtt ggcttcctgg gctttagtct ggcgatcatg ggttgggtct
       61 ttcctgcttg tgcgcaagat actgtcaatc cgcaagggcc gccaaatgcg tttgtgcagg
      121 ctgtgggcaa caatgcactg aacgcggtgc ggggcgacgc cgcggcaaag agcggcgatc
      181 tgcgccgcat caacgagctg gtcaatcagt atctgctgcc ctacgttaat ttccaaaaaa
      241 cgacgcagtt ggctgcgggt caatattggc gttcggctac tgcacaacaa aagaaagacc
      301 tggccgaggc gtttaaaggc acgctgatcc gcacttatgg cggcgccctg aatgatgtcg
      361 acaagattgc aggcctcaat atattgccat ttcggggcga tgcaagcgcc aatgacgtgg
      421 tcgtgcgttc gacgttctcg caacgcaatg gcccgtcgat cggggtggac tatcgcctgg
      481 aaaagtcgtc tgatggctgg aaaatttacg atttgaatgt tgaaggcatt tggcttatcc
      541 agaattacag gaatcagttt tcgcagcaaa tcgcccaaaa tggaatcgac ggattgatct
      601 cggcgttaaa tcagaaaaat aactagcttt gccgaaatga aaaaagggcc aacggcccct
      661 gttccactga aaaccggact gtagcaatgc tacgtccatg gtaccgaatc tggagcggga
      721 gacgagtctc gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctac
      781 tcccgcgttt gtttttgttt tttacatgcc taaaacaaaa cagcctcgca gtatatctta
      841 acttccaacc gcgagcaaac ctgaccggct tgcaatgcac cgtgtgcctt tggaaataaa
      901 gtaacatgct gcgaagtccg aaataatagc atactcgttg cttcgtttac cgaacctttc
      961 aatcatcagg cccatggtgt tgtccgctac tacccaagat cttacgtcgt tcaatacctt
     1021 gcaattgcat tcccggacgc gcgcactggt gcgttacagg tcacctgccg aattggccga
     1081 tttgtccggt ctggcaaatc agtatcgtca tgttttcgtg ttgggaggcg gcagcaatgt
     1141 ggtgttggcc gaacatctga gatgccttgt gatcaaggtg gagtcgcgcg gcgtgcggat
     1201 actgcaacag gatgaccatg agctgattat tgaggcgcaa gcaggcgagg tttggcccga
     1261 ttttgtcagg ctttgcgtgg ccaggggctg ggatgggctt gagaatctgg cgttgattcc
     1321 gggaacggcg ggggcggcgc cggtgcaaaa tatcggggct tatggcgtcg agcttgagca
     1381 gcggtttcat agcttgtcgg tctgggattt gccgaatcgg aaaatgcttg aaatgggtcg
     1441 gcacgactgc aagttttctt accgcgacag tttattcaag catgccgagc cggggcgttg
     1501 gctaattacg gccgtgcgtt ttcgcctgcc caagccatgg aaacctgtgc tggagtattc
     1561 cgatctgaaa cgtcatcccg tgttgatggc cccgaatcag gctgtaaccg ccacgcaaat
     1621 ttttgacgcc gtttgcgaaa ttcgccgcgc caagttgccg gatccggcgc acatcggcaa
     1681 tgcgggaagc ttttttaaaa accccatagt gaccgttgcc cgttataacg caatcaaggc
     1741 tctgtatccg ggtcttgtgg cctatccgca agacgggggc ctggtgaaac tggcggcggg
     1801 ctggctgatc gatcaggctg gatggaaggg ccggcgcatg ggtaaagtgg gtgtgcacga
     1861 tcgccaggcg ctggtgctgg tcaattatgg tggggctaat gcggacgata tcgccttgct
     1921 ggcccgagct gtgcgtaccg acgtggccac gcgttttggc gttcaactgg aacaggaacc
     1981 gattcgggtg gcttgaacca ggcgctcgcg cgggtgcatt gataaggacg gcgctggcgc
     2041 gctgttaaat ggcaagtacg tcttgcatat cgaacaggcc agtttgcttc cgcgacaggt
     2101 agcgtgctgc gcgcaagctg ccttgtgcgt aggttgaacg gctgctggac cggtggctga
     2161 tttcaatgcg ttcgcctgtg ccgcagaaag aaaccgtatg gtcgccta
//
LOCUS       DATCXU010000205         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_712601,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000205 DATCXU010000000
VERSION     DATCXU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712601"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1156
                     /locus_tag="VIR04_04595"
     CDS             71..1156
                     /locus_tag="VIR04_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460058.1"
                     /translation="MLHRLALRVLPSLTLAVTDDSVRKRKRLVMFVPGLVAFAAYRAA
                     KQGFSLAEPMTLLLLSGLVSMVTAACAYRVGRALPFTRLVAVDGAQRIGWILAWIGFV
                     YGIQLSLLVLALLWLVGYDYAMHPDGPAMMAIIIPCTAVARDAFEIGHVRWLQHSGRP
                     FPTFPDGAGLRTLLKRLNSTVLSWIAVGALGSASVSVVMATAVSGDMAVLAQGAIVTI
                     AAGTIALLAFFAGQAGGEAWVSRVGATSWGELMKFWWWPGLAFASTYYLVVIGGCLYV
                     LKQPLSSVGLFAGAAALVGGLMSLYCYYLGYRRDVENREQGGVPSALLRCPFVMGILG
                     KSRETISNAALNGSAAVFEKNSQRASS"
     gene            1153..3021
                     /locus_tag="VIR04_04600"
     CDS             1153..3021
                     /locus_tag="VIR04_04600"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY9460059.1"
                     /translation="MTKRLPFRIRIVIFLCWAVLTATAAFLNPGPLAAADSSTDVYFQ
                     TPGVPSGPPAPTGNESHYPYYGSLDSRLLMWLIIQQHTYFGGFVLALPIFCVLLEFLG
                     LVARNPALALRYDGLAQDLLKVALLAISVTAAVGSLMLTMLITLYPSFMQYMGGTFKG
                     MMPLYALVFVGTTFFTIAYYYSWDRMSAPGVKWIHISIGLIAIAFGTSLLLLANAWSA
                     FMMAPSGVDGQGRYLGNPWHLLRSALWNPLNLHRFLADIMSGGAVVLAYACYRFFTGK
                     SQEERAYYDWVGYVFLFVTVCALLPMPFAGYWLMRSVYTYSQSMGVTMMGGLLTWLFV
                     VQALLIGALFLGVNYYLWQSMGRIRGGERYQPYYQYLLLALMGCLFIWLTPHTILMTG
                     TEVKAMGGAQHPVIGNYGVMSSKNGAVNVMICITTLSYIYYRRANRTMTISWAKTGNI
                     MLGALFGLGVAHIIGLSVYGFYLPASVRVAMSGPQAVTTLLIVTLGLLLNRRMLQGAT
                     IHGSVQWGQISMRGMVGLFGLAAAFTWVMGLMGYIRSSGRLAWHVNELMPDTSPWAFT
                     PTLGFAAKMVTINMVVFWGAVFFLFWVCQRGRQPVMHEDISAEKEAGFLPSPSHEG"
     gene            3057..3707
                     /locus_tag="VIR04_04605"
     CDS             3057..3707
                     /locus_tag="VIR04_04605"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY9460060.1"
                     /translation="MMGKVVWIMGLVALWGGMEAGTRGPFAEAGTLVLEDFQSADQEG
                     FPIDWQHENQRSQAKGREAYKIQSENGQKFLAAKDAGQRVKNRKIDWDPKAYPVLTWR
                     WRLHKAPSGSEPIAAVFASLDTDLIFIPVFTKYIWSGAKPEGTIIEGGMFSGSEIVVQ
                     SGVHPVGEWVEERINVYEDFKRLHQHEPAEKAWGISLFAGPGVEIDFGKISVSTAN"
     gene            3742..>3937
                     /locus_tag="VIR04_04610"
     CDS             3742..>3937
                     /locus_tag="VIR04_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460061.1"
                     /translation="MTAKSRPSTRVLFDVVFGVMVMGTVGALIGTILGGSSVPFASGF
                     GLVLGAVVGFLGGRRFLVSIL"
BASE COUNT          685 a          955 c         1295 g         1002 t
ORIGIN      
        1 ggcggcgcag cgaggtgaag cgtgaacacc gtctcttgcc cctaatctct cacttccaac
       61 ggaaaacaca atgttgcatc gactggcatt acgagttctg ccgagtttga cgttagccgt
      121 cacggacgat tcggttcgca aacgaaagcg cctggtcatg ttcgtgccag ggttggtggc
      181 cttcgctgca taccgggcgg caaaacaggg attttcgctg gcggaaccga tgaccttgct
      241 tttgctgagt ggattggtat cgatggtgac ggctgcttgc gcctatcgtg tggggcgcgc
      301 gttgccgttc acgcggctgg tagcagtgga cggagcccag cgcatcggtt ggatactggc
      361 ctggatcggc tttgtgtacg gaattcaact gtctctcctg gtgctcgctc tgctgtggtt
      421 ggtagggtat gactatgcca tgcatcctga tgggcctgcg atgatggcga tcattattcc
      481 ctgcacggcg gtagcgcgtg atgcgtttga gattgggcac gttcggtggc ttcagcacag
      541 cgggcgtccc tttcccactt tccctgacgg cgcggggtta cgtacccttc ttaaacggct
      601 caattcgacg gtgctgagct ggatcgccgt gggggctctc ggaagcgcaa gcgtttcggt
      661 ggtcatggcc acggccgtga gtggagacat ggcggtgctg gcgcaaggtg ccatcgtgac
      721 gatcgcggcg ggaaccatcg ccctcttggc gtttttcgcc gggcaagcgg gtggggaagc
      781 ctgggtgtcc agggtgggcg cgaccagctg gggagagttg atgaagtttt ggtggtggcc
      841 tgggctggct tttgcctcga cttactatct ggtggtcatt gggggctgcc tctacgtgtt
      901 gaagcagccg ctgagttctg ttggattatt cgccggggcc gccgcgctgg tcggtggctt
      961 gatgtcgctg tactgctatt acctgggtta tcggcgcgac gttgaaaacc gcgagcaggg
     1021 aggagtgccc agcgccctgt tgcgctgccc gtttgtcatg ggcattctcg gtaaatcccg
     1081 tgaaaccatt tcaaatgcgg cattgaatgg atctgcggct gtttttgaaa agaacagtca
     1141 gagagcgtcg tcatgactaa acgccttccg tttcgaataa ggatcgtcat atttttgtgt
     1201 tgggcggtgc tgactgcaac cgccgcattc ttgaatccgg ggccgctcgc ggcggcagat
     1261 tcctccacgg atgtatattt tcagacgccc ggcgtcccta gcggcccccc cgcgccgact
     1321 ggtaatgaga gccactatcc gtactatggt tcgctcgaca gccggttgtt gatgtggctg
     1381 atcatccagc agcacaccta ttttggtggt ttcgtattgg ccttgccgat cttttgcgtt
     1441 ctgttggagt ttctggggct ggtcgcacgg aatccggcac tggccttgcg gtatgacggg
     1501 ctggctcaag acctgttgaa ggtggcgctg ctggcgattt ccgtcactgc ggcggtcgga
     1561 agcttgatgc tgacgatgct tatcacgctc tatccgagtt tcatgcagta catggggggg
     1621 acgttcaagg gcatgatgcc cctctacgcc ctcgtgttcg tcgggaccac atttttcacg
     1681 atcgcgtatt actacagttg ggatcgtatg tcggcgcccg gggtgaagtg gatccacatt
     1741 tcgatcggtc tgattgcgat tgccttcggg acgtcgctgc tgttgcttgc caatgcgtgg
     1801 tccgcattca tgatggcacc atccggagtc gacggacagg gccggtattt gggcaacccc
     1861 tggcatctgc tccggtcggc cctctggaat ccgttgaacc tccaccggtt tttggcggat
     1921 atcatgtcgg gcggcgcagt cgtgctggcc tatgcttgtt accgattttt caccggcaaa
     1981 agtcaggaag agcgtgccta ctatgattgg gtcgggtatg tgtttttgtt tgtgacggtc
     2041 tgtgctctgt tgccgatgcc gtttgccggc tattggttga tgcgctcagt gtatacatac
     2101 agtcaaagca tgggtgtcac gatgatgggc gggttgctga cctggctctt cgtcgtgcag
     2161 gcgctcttga tcggcgcgct ctttctcggc gtgaactact atctttggca aagcatgggg
     2221 cgaatccgag ggggagaacg ctatcagccc tactatcagt atcttctgct ggcgctcatg
     2281 ggatgcttgt ttatctggct gacgccgcat acgattctca tgaccggcac ggaagtgaag
     2341 gccatgggcg gggcgcagca tccggtcatt gggaactatg gcgtcatgtc gtcaaagaat
     2401 ggcgcggtca acgtgatgat ttgcatcacc accttgagct atatctacta tcgtcgcgcg
     2461 aatcgcacga tgaccatttc ttgggccaag accgggaaca ttatgttggg ggcgttgttc
     2521 ggtctggggg tggcgcatat catcgggttg tcggtgtatg gattttatct tccggccagc
     2581 gttcgtgtgg ccatgtctgg tccgcaggcg gtaacgacgt tgctgatcgt cacgctgggg
     2641 ttattgttga accgtcgcat gttgcaaggc gcgacgatcc acggctctgt ccagtggggg
     2701 cagatctcga tgcgtggaat ggtcggattg tttggattgg ccgcggcgtt tacctgggtg
     2761 atggggttga tgggctacat tcgctcttcc ggccggttgg cctggcatgt caatgaattg
     2821 atgcccgata cttcaccctg ggcatttacg ccgacgctcg ggtttgccgc gaaaatggtg
     2881 acgatcaata tggtcgtttt ttggggcgcc gtctttttcc tgttttgggt ctgccagcgc
     2941 gggcggcagc cggtgatgca tgaggatatc agcgcggaga aagaggcggg ctttttgccg
     3001 tctccatcgc atgagggata ggtggtacat cagggactgt ggagatcgct atgaggatga
     3061 tgggcaaggt tgtatggatc atggggttgg tggcactgtg ggggggaatg gaggctggta
     3121 cgcgaggacc gtttgccgag gccgggacgc tggtattgga agattttcag tcggcggacc
     3181 aagaggggtt tccgatcgat tggcagcacg aaaatcagcg gagccaggcc aaagggcggg
     3241 aggcttacaa gatccaatcc gagaacgggc agaagtttct tgccgccaaa gatgcgggac
     3301 agcgcgtgaa aaatcgcaaa atcgattggg atccgaaggc ctatccggtg ctgacatggc
     3361 ggtggcgtct ccataaagcg ccgagtggat cagagccgat tgctgcggtt tttgcctcgt
     3421 tggatacgga cctgatattc attcccgtgt ttacgaaata catctggagt ggggcaaagc
     3481 ccgagggcac gatcattgag ggcggcatgt tcagcgggtc ggagatcgtc gtgcaaagcg
     3541 gagtacatcc agtgggtgaa tgggtcgaag agcggatcaa tgtctatgaa gatttcaagc
     3601 ggcttcatca acatgagccg gcggagaaag cctggggaat ctcactgttt gccgggcccg
     3661 gtgtggaaat tgattttggc aagatttcag tgagtacggc caactgagcg tctgcgcggt
     3721 cctcccgatg aaagggattc tatgacagcg aagagtcggc catcgaccag ggtcttattc
     3781 gatgtggtgt ttggcgtcat ggtcatgggc acggtcgggg cattaatcgg aacgatcctg
     3841 ggtggctctt cggtcccctt cgcctctggg tttgggttgg tactgggcgc ggtggtcggc
     3901 tttctcggtg ggcgtcgatt ccttgtcagt atcctgg
//
LOCUS       DATCXU010000206         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_500856,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000206 DATCXU010000000
VERSION     DATCXU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500856"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /gene="tyrS"
                     /locus_tag="VIR04_04615"
     CDS             complement(<1..636)
                     /gene="tyrS"
                     /locus_tag="VIR04_04615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY9460062.1"
                     /translation="MLPFDAPVTAEVEADLRIAKRGCDELLVESEFVKKLALSRAKGT
                     PLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLGHKVIFLIGDFTSMIGDPSGRNVTRP
                     PLTAEQIHENASTYYAQASLVLDPAKTEIRYNSEWSDPLGARGMIELASRYTVARMME
                     REDFTRRFKGGIPISVHEFLYPLMQGYDSVALKADLELGGTDQKFNLLVGRE"
     gene            763..2145
                     /locus_tag="VIR04_04620"
     CDS             763..2145
                     /locus_tag="VIR04_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY9460063.1"
                     /translation="MFKKGTTDTPYSTHLFRARNHTPVFIRNGLVLVALGLSAAAALA
                     MVTPPQDATPTLAYTVRQTLSLPGQTPELADRYSKPFISETQIRSGDVLAAVIQRLGI
                     REPELQQFLVNNKEARSIYKLYPGRTVLAALDQNGQLVWLRYNHTPGQYQQGEYVSKW
                     LEVRPDGQGSFTAQEHSQAASMHVRIAEGEIDSSLFGAADEANIPDAITQQMTDILGS
                     KIDFMKDLRQGDRFRIVYESYSHAGKTVGAGRILALEFINGDHAYEAVWFDQNGDNRP
                     GSYYDFSGHNLKGAFLRTALAFTRISSTFGRRLHPLHGTWKSHDGVDYAAPRGTPIHA
                     TADGTVEFIGKQSGYGNVIILAHGGHYETLYAHQQAFAKGLKKGATVAQGDVIGYVGA
                     TGWATGPHLHYEFRVDEKPVNPLSIDLPVAQALDQRQLPLFERAVAQYKDHIRLLAQL
                     QSQDVQLANL"
     gene            2170..>2719
                     /locus_tag="VIR04_04625"
     CDS             2170..>2719
                     /locus_tag="VIR04_04625"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY9460064.1"
                     /translation="MAPLSLFVGLMSGTSTDGVDAVLADLSDLAHPEIKAHAALPMPE
                     VLRREFFDLNNAGENELMRAALAGNALARLYAQAVRRLLDETGLQAGDILAIGAHGQT
                     VRHDPAAGYSIQINNPALLAELTGIDVIADFRSRDVAAQGQGAPLVPAFHKALFSSDH
                     PRVVLNLGGIANVTLLNPRKEVQ"
BASE COUNT          646 a          789 c          723 g          561 t
ORIGIN      
        1 ctcgcgtccc accagcagat tgaatttctg gtcggtgccg cccagttcaa gatccgcctt
       61 aagcgccacg gagtcgtaac cctgcataag cggatataaa aactcgtgca ccgaaatggg
      121 aatgccgcct ttgaaacggc gcgtgaaatc ttcgcgttcc atcatgcggg cgactgtata
      181 gcgcgaggcg agttcaatca ttccgcgcgc gcccagcgga tcgctccatt ccgagttata
      241 gcggatttct gttttggcgg gatcaagcac cagactggcc tgtgcataat atgtgctggc
      301 gttctcgtga atttgttcgg ccgtcagcgg cggacgcgtt acgttgcgac cgctgggatc
      361 gccgatcatc gacgtgaaat cgccaatcaa aaaaatgacc ttgtggccca aatcttgcaa
      421 ttggcgcatt ttattgagca ctacggtgtg tcccagatga atatcgggag ccgtggggtc
      481 caggcccagc tttatacgca ggggcgtgcc cttggcgcgg ctgagagcaa gttttttaac
      541 aaactcggac tcaaccagga gctcgtcgca accgcgcttc gcaatacgta aatcagcttc
      601 cacttcagcg gtcacaggag cgtcaaaagg taacataaca aaccgtttta ggataaaagg
      661 taaaattgct cgaaaggttc tgcgtaatac gttaggataa cgcccaaacg cggcagagcg
      721 cactcatcca aaatcagcac accaccacca ggctttagct ttatgttcaa aaaagggaca
      781 actgacactc cctattccac acatctattc cgcgcacgca atcatacccc ggtcttcatc
      841 cgaaatggct tggtcctggt agcgttgggt ctatcggccg ccgccgctct ggccatggtc
      901 acaccgcccc aagatgcgac acccacactt gcctataccg tcaggcaaac gctgtcgtta
      961 cccggccaaa cacccgaatt ggcggatcgt tattccaaac cattcatcag cgaaacgcaa
     1021 atccgtagcg gcgacgtact ggcggctgtc atacagcgat tgggtattcg ggaacccgaa
     1081 ttgcaacagt tcctggtcaa taacaaagaa gcgcgatcca tctacaaact ttatccgggc
     1141 cgcacggtgc tcgcggccct cgaccaaaac ggtcaactcg tctggctgcg ctataaccat
     1201 acgcccggtc agtaccaaca gggtgaatat gtctccaaat ggctagaggt gaggcccgac
     1261 gggcaaggca gctttactgc acaagaacac agtcaggcgg caagcatgca cgtacgtatt
     1321 gccgagggcg agatcgacag ttcgctattt ggggccgccg acgaggcgaa catccccgat
     1381 gcgattacgc agcaaatgac tgacattctt ggcagcaaaa ttgacttcat gaaagacttg
     1441 cgccaaggcg atcgctttcg cattgtctac gaatcgtact cgcatgccgg caaaacagtt
     1501 ggcgcgggcc gcatccttgc gctggagttc attaatggcg atcacgccta tgaagcagtc
     1561 tggtttgatc agaatggcga taacaggcca ggaagctact acgacttcag cggtcataat
     1621 ctgaagggcg cctttctacg cactgcgctc gcttttacac gcatcagctc gaccttcggc
     1681 aggcgcctgc accctttaca cggcacctgg aaaagccacg atggggtgga ctatgccgcc
     1741 ccacgaggca cgcccatcca cgctacggct gatggcaccg tggaattcat cggaaaacaa
     1801 tccggctacg gcaatgtcat tatcctggcg catggcggtc actacgaaac cttgtacgct
     1861 catcaacaag cctttgccaa aggcctgaaa aagggcgcga cggttgcgca aggcgatgta
     1921 atcggatacg ttggcgcgac gggttgggca accggcccgc atctgcatta cgaatttcgc
     1981 gtcgatgaaa aacctgtaaa ccctttatcg atcgatttgc ctgtggcgca agcgctggat
     2041 caacgccaac tgcccttgtt tgagcgcgcc gtcgctcaat acaaagacca cattcgcctg
     2101 ctcgcccagt tgcaaagcca ggacgtccaa ctggcaaacc tctgacgcaa ccggcgttca
     2161 ctatttcaaa tggcgccgct ttccttattc gtgggcttga tgtcgggcac cagcaccgac
     2221 ggtgtcgacg ccgtgctggc ggacttgagc gatttggccc accccgaaat aaaggcgcac
     2281 gcggcattgc ccatgccaga ggtcttgcgg cgcgaatttt ttgacctaaa taacgccggc
     2341 gaaaatgaat taatgcgcgc ggccctggcg ggcaatgcac tagccaggct gtacgcccag
     2401 gccgttcgcc ggctgctgga cgaaacaggc ttgcaggcgg gcgacatact ggccattgga
     2461 gctcatggac aaaccgtcag gcacgatccc gcggcgggct attcgatcca gatcaacaac
     2521 cctgcgctgc tggccgaact gacgggcata gacgtcatcg ctgacttccg ctcacgggat
     2581 gtcgccgcgc aaggccaggg cgcaccattg gttccggcct ttcacaaggc attattcagc
     2641 agcgatcacc cgcgcgtggt tctaaatttg ggcggcatcg cgaatgtgac cttattgaat
     2701 ccgagaaaag aggtgcaag
//
LOCUS       DATCXU010000207        11113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1049520,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000207 DATCXU010000000
VERSION     DATCXU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049520"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VIR04_04630"
     CDS             complement(<1..1019)
                     /locus_tag="VIR04_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY9460065.1"
                     /translation="MVKGYAIGRAVVMGAAALEVAHYRVHPDNVQSECERLRKAVNDA
                     CDELQSMASGLSDDAPRELGALLTVHGMLLADPMLSQLACDLITQRHYNAEWALTTQG
                     QILGEQFAAMEDAYLRERGADIRQVIERVLRVLSGSTTLLPDLSTLDSDDCLIVVARD
                     ISPADMLRLRGGRFGAFLTDLGGPTSHTAIVARSMNVPAVVGLGHIRALVRDGDILIV
                     DGSTGAVLINPPLNVLNEYRRRQEAFADERAELGLLRDAPAITLDGIMIRLEANIELP
                     EEAVAALAAGADGIGLFRSEFLFMGRTDLPGEDEQYQAYASVVKTMAGRPVTIRTLDL
                     GSDKSL"
     gene            complement(1109..1378)
                     /locus_tag="VIR04_04635"
     CDS             complement(1109..1378)
                     /locus_tag="VIR04_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY9460066.1"
                     /translation="MPVIDIVICNKLGLHARAAAKLTQIAGKFRSDIFIARGAQRVNA
                     KSIMGVMMLAAGLGVTVKVEASGDDADQALVAIQSLFDKKFGEHE"
     gene            complement(1430..1822)
                     /locus_tag="VIR04_04640"
     CDS             complement(1430..1822)
                     /locus_tag="VIR04_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY9460067.1"
                     /translation="MARIVLIMHAPLGSAFSECAGHILGQKPNLTVFDIKPDDRPDLR
                     VEQLMRWIRQRPSETSLLLCDIFGATPFNIASQALKRAAEQGSVGHLLTGTNLCMVLK
                     ALTDQQENPDQLSENVRLGALKGIIDAV"
     gene            complement(1823..2782)
                     /gene="gshB"
                     /locus_tag="VIR04_04645"
     CDS             complement(1823..2782)
                     /gene="gshB"
                     /locus_tag="VIR04_04645"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY9460068.1"
                     /translation="MHVLFVIDPLFSLKAYKDSSVAMMRALTARGHRLSVALQGDLYV
                     DHGAVKAASTAIELIAGADLHGHSWWRETGSKHDAELASFDAVLMRKDPPFDMEYVYA
                     THLLGYAQAQGARVFNSGEAIRNHPEKLAITEFASFAPPTLVSRDMARLRAFHAAHHD
                     VIVKPLDGMGGTGVFRLQKAEPNLGVILETLTDNGSRTIMAQRYIPEIVNGDKRILLI
                     AGEPVPYALARMAPEGETRANLAAGGNGIARPLSARDREIAQIVGPTLAARGLLLVGL
                     DVIGDYVTEVNVTSPTCFVEITEQTGFNVAECFVAALERAAGA"
     gene            complement(2842..3333)
                     /gene="infC"
                     /locus_tag="VIR04_04650"
     CDS             complement(2842..3333)
                     /gene="infC"
                     /locus_tag="VIR04_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY9460069.1"
                     /translation="MGVDGEQLGVVKTQDAIHLSEQNEVDLVEIAPTAEPPVCRLMDY
                     GKFKYQEQKRQAEARAKQKVIQVKEVKFRPGTDEGDYQVKLRNLRRFIEEGDKAKVTL
                     RFRGREMAHQELGMRVLERVRDDLVELCQVEAMPKLEGRQMVMVLAPKKKAAPHKQGE
                     SAS"
     gene            complement(3434..5371)
                     /gene="thrS"
                     /locus_tag="VIR04_04655"
     CDS             complement(3434..5371)
                     /gene="thrS"
                     /locus_tag="VIR04_04655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY9460070.1"
                     /translation="MVRITLPDSSQREYPAPVTVGDVAHSIGAGLGRAALGGRVALNG
                     DASRLVDTSFRIEQDARLAIVTAKDPDGLDLIRHSTAHLLAYAVKLLFPDAQVTIGPV
                     IENGFYYDFSYKRPFTPEDLVAIENKMVELAKKDETITREEWSRDDAVKYFNGIGEHY
                     KAEIIGSIPQDQTISLYREGEFIDLCRGPHVPSTGKLKVFKLTKLAGAYWRGDSNNEM
                     LQRIYGTAWASKEDQAAYLTMMEEADKRDHRKLGRELDLFHFQDEAPGLVFWHARGWA
                     LWQQVEQYMRQVYRENGYQEVKAPQILDLSLWKKTGHWDNYAENIFTTESENRVYGLK
                     PMNCPGHVQIFNAGLHSYRELPIRYGEFGQCHRNEPSGSLHGLMRVRGFTQDDGHIFC
                     TEDQLQDECAAFTALLQKVYADFGFTHILYKVATRPDKRIGADEVWDKSEKALMESLD
                     RTHCEYEISVGEGAFYGPKIEYTLKDAIGRHWQCGTIQVDFSMPQRLGAEYVDANDQR
                     KAPVMLHRAILGSFERFIGILIENHAGAMPPWLAPEQVVVCCISDPSADYAAEVAQSL
                     KAQGFRVNSDLRAEKITRKIREHSLRKIPYILVVGDKEREARTVSVRSRGNVDLGVMP
                     LAAFADRLQQDIDTRRDVGPA"
     gene            5742..5818
                     /locus_tag="VIR04_04660"
     tRNA            5742..5818
                     /locus_tag="VIR04_04660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5776..5778,aa:Val,seq:gac)
     gene            complement(5867..6646)
                     /locus_tag="VIR04_04665"
     CDS             complement(5867..6646)
                     /locus_tag="VIR04_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460071.1"
                     /translation="MKHVLASTLLVAPALVFAQTSAAPSDTLSHVLESHTLRVCSPGD
                     YKPFSFQKADGQFEGLDVDLMASLAGALGAKPAFVKTTWAKLMDDFTSGKCDIGAGGI
                     SVSLDRQKKAYFSSAYMINGKTPITLCTNVKKFQTIADINKPDVRVIVNPGGSNEKFA
                     KTNLSKARITTHENNIGIFKEVAEGRADVFVTEAAEAKVQSKLNPRLCAVNPDKPLQY
                     AEMAYLLPAGDMRFKLFVDQWLHLSKAGGEYARLANKWVPQ"
     gene            6814..7659
                     /locus_tag="VIR04_04670"
     CDS             6814..7659
                     /locus_tag="VIR04_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY9460072.1"
                     /translation="MITHPFGNTNRNIAAVGQGTWDIDTAQRDQAIATLRRGLDLGMS
                     HIDTAEMYGSGAAEILLGEAIKGRRDEVFLVSKVLPNNASRAGTRAACERSLRQLRTD
                     CLDCYLLHWRGSYPLEETFAAFEDLQRDGKIRSFGVSNFDVDDLDEAFQLLGPGKLAC
                     NQVLYHLQERAIEHSVIPWCERHGLAVVAYSPLGHDNFPSPDTASGRVLGKVARAHGA
                     TPHQIALAFLTLRPSIFAIPKASSVTHVEDNAASADINLSGAEVRLIDEAFPRGPKPS
                     SLPMI"
     gene            complement(7712..8530)
                     /locus_tag="VIR04_04675"
     CDS             complement(7712..8530)
                     /locus_tag="VIR04_04675"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY9460073.1"
                     /translation="MGHVRTLSQAWQHALDDAHTQDVLLSLDHHLADQLARHTQIFPL
                     RPFRALLEVEPQDVQVVILGQDPYHGPNQAQGLAFSVPDACPAPPSLRNIFAELAREY
                     QDTFVRQRNSLIRWARQGVLLLNTSLTVEARKPASHANQGWEHVTDTILACVLREARP
                     KVFLLWGSHAQSKQALLRESYAKGPLLALKANHPSPLSALRPPRPFIGCGHFQKANAW
                     LQENGQPGIDWLQNKTPAARDKPAISGNVQQKAPEAQIEPPPAMGPAGVQGSLW"
     gene            complement(8617..>11113)
                     /locus_tag="VIR04_04680"
     CDS             complement(8617..>11113)
                     /locus_tag="VIR04_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9460074.1"
                     /translation="RMAAHGVTAADVYAALGNNNTLAALGATKGQMVSVDMTANTDLH
                     SVDEFKQLVVKSHSGALVRLQDVADVVLGADNYDFSVAFSGRRSVFIGIKTAPEANVL
                     DVAKRVRDAFPAVQSQLPTGLTGKIVYDATDFVNTSINEVVKTLIEALVIVTVVIFLF
                     LGSFRAVIIPVIAMPLSLIGAFFVMLTLGYSINLLTLLALVLAIGLVVDDAIIVVENV
                     DRHMKEEGKPPLQAALLAARELGGPILAMTVVLVAVYVPIGFQGGLTGALFTEFAFTL
                     AGAVTVSGVIALTLSPMMCSRFFRADQESGRFARYIDRQFERVHRGYQRLLHSLLDTW
                     VVLVVMGALLLAGTAYLFMTSKSELAPQEDQGIVLSQIQGPPNATIQQMNTYADQVFH
                     IAKGIPEYQQMFQLTGVPAVNRGIGGVLLKPWDERKKGAAQLQQELQAKWNTIAGARV
                     AAFQFPPLPGTQGLPVQFVITTTEPFQNLNDVSQAVLAKARASGMFFFVDSDLKIDKP
                     ESTLVVDRDKVASLGLMQADVGNALTAALGGGYVNYFSIAGRSYRVIPQVLQVDRLNP
                     SQILDYYLHTPNGGLIQASTVAYLESHVAPESINHFQQLNSATISGVAVPGASEGDVL
                     SYLSQATQQAAPSGYSIDYSGASRQFAHESGGFVYTLLFATIIVFLALAAQFESFRDP
                     VVILVSVPMALFGALLFINIGFASLNIYTQVGLVTLMGLISKHGILIVQFANELQRAG
                     YNKRDALEQAAAVRLRPILMTTAAMVLGVSPLVFASGAGAAGRHAMGLVIFTGLSIGT
                     LFTLFVVPAMYLFMAADHHHEARQTGVTGGHLA"
BASE COUNT         2479 a         3312 c         2986 g         2336 t
ORIGIN      
        1 agagatttat cggagccaag gtccagcgtg cggatcgtga cgggccggcc tgccattgtc
       61 ttcacgaccg acgcataagc ctgatattgt tcatcttcgc ccggcaggtc ggttcgtccc
      121 atgaacagga actcgctgcg aaatagaccg atgccgtcgg cgccggccgc caaagccgcg
      181 acagcctctt cgggaagctc gatgttggcc tcgagccgga tcataatgcc atcaagcgtg
      241 atggccggcg catcgcgcaa cagcccaagc tcggcgcgtt catccgcaaa cgcctcctgg
      301 cggcgccggt attcattcag cacgttgagc ggcggattga ttaacaccgc gccggtcgaa
      361 ccatccacta tcagaatatc gccatcgcgc accaaggccc gaatgtgccc caatcctacg
      421 acagcaggca cattcatgct gcgcgccaca atagccgtat gcgaagtggg ccctccgagg
      481 tcagtcagaa aggcgccaaa gcgcccgccg cgcaaacgca acatatcggc gggagaaatg
      541 tcgcgcgcca caacaatcag gcagtcgtcg ctatcgagtg tcgacagatc aggcaacaac
      601 gttgtggatc ccgacagaac gcgcaacacg cgttcgataa cttgtcgaat atcggcgccg
      661 cgttcgcgca aataggcgtc ttccatagcg gcgaactgtt cgcccaatat ttggccctgc
      721 gtggtaagag cccactcggc gttgtagtgg cgctgcgtga tcaggtcaca ggccaattgc
      781 gacagcatcg ggtcggccag aagcatgccg tgcaccgtca ataaagcgcc caattcgcgc
      841 ggagcatcgt cggacaaccc gctggccata ctttgcagct catcgcaggc gtcattcacc
      901 gctttgcgca atcgctcgca ttcactctga acattgtccg ggtgaacgcg gtagtgcgcg
      961 acctccaggg ccgccgcccc cataaccacc gcgcgcccga tcgcgtagcc tttaaccacg
     1021 ccctgccctt gcaagcaaag cattgagcca agcgagggtg cggcagaagg aatggaaacc
     1081 attattacat tacccagcgc aggcagcgct attcgtgttc gccaaacttt ttatcgaata
     1141 aagactggat ggccacaagc gcctgatcgg catcatcgcc cgacgcctca accttcacgg
     1201 tgacgcccag ccccgccgcc agcatcatga cgcccataat gctcttggcg ttgactcgct
     1261 gagcgccgcg cgcgataaag atatcgctac gaaacttgcc ggctatttga gtcaatttgg
     1321 ccgccgcccg ggcatgcaag ccaagcttat tacagatgac aatgtcgata actggcatag
     1381 agctctttat gtcgatgcgc gcgaccagcg cgcgtaatat tgagaatgtt cagactgcat
     1441 cgattatgcc tttcaaagct cccaaacgca cattctcact gagttgatca gggttttcct
     1501 gttgatcggt aagagcctta agcaccatgc ataaattggt gcccgtcagt aaatgcccaa
     1561 cggagccctg ctcggccgct cgtttaagcg cctggctggc aatattaaaa ggcgttgcgc
     1621 caaaaatatc acaaagaagc aagcttgtct cggatggccg ctgccttatc caccgcataa
     1681 gctgttcaac gcgcagatcg ggccggtcgt cgggcttgat gtcgaaaacc gtcagattcg
     1741 gcttttgccc aagtatatgc ccggcgcact ccgaaaacgc gcttcccagc ggcgcgtgca
     1801 taatcaatac gatgcgggcc atttatgccc ccgccgcccg ctctagcgca gcaacaaagc
     1861 actcggctac gttaaaaccg gtttgctcgg ttatctcgac aaaacaggtc ggactggtga
     1921 cgttgacctc ggtaacataa tcgccaatga catcgagccc caccaatagc aaaccgcggg
     1981 ccgccagggt cgggcccacg atttgagcaa tctcgcgatc gcgcgccgaa aggggccgcg
     2041 ctatgccatt gccgcccgct gcaaggttcg cgcgcgtttc gccttccggc gccatccgcg
     2101 ccaacgcgta aggcacgggc tcgccggcga taagcaaaat gcgtttgtcg ccattgacaa
     2161 tttcaggaat atagcgctgc gccatgatcg tacgcgaacc gttatcggtc agcgtttcca
     2221 gaataacgcc cagattgggt tcggccttct gcaagcgaaa aacgccggtg ccgcccatgc
     2281 catcgagcgg tttgacgatc acgtcgtgat gcgcagcgtg aaacgcgcgc agacgagcca
     2341 tgtcgcgcga caccaaagta ggcggcgcaa aagacgcaaa ttcggtaatg gcaagctttt
     2401 cgggatgatt gcggatggcc tcgcccgaat tgaatacgcg ggccccttgc gcctgagcgt
     2461 atcccaacaa gtgcgtggca tacacgtatt ccatatcgaa cggaggatcc ttgcgcatta
     2521 gaaccgcatc aaacgaggcc aattccgcgt cgtgcttcga ccctgtctcg cgccaccacg
     2581 aatgcccatg caagtccgca ccggcaatca attcgatcgc cgtgctggcc gctttaaccg
     2641 ccccatggtc aacatacaga tcgccttgca acgcaacgct taaacgatgg ccacgggcgg
     2701 tcaaagcacg catcattgca accgacgagt ccttatacgc cttcaacgaa aataaagggt
     2761 caataacaaa caatacatgc ataacgcacc ctcgagcgcc cgcgtgccag aagccgcccg
     2821 acagcgcgaa ttaaaacaat ttcaggacgc tgattcgcct tgcttatgag gcgcggcctt
     2881 cttcttcggc gccagcacca taaccatctg acggccctcg agcttgggca tggcctcgac
     2941 ttggcagagt tcgaccaggt cgtcgcgcac ccgctcaaga acccgcatgc caagttcctg
     3001 atgagccatt tcacggcccc ggaatcgcaa agtgaccttg gccttgtcgc cttcttcgat
     3061 gaagcggcgc aaattacgca atttgacttg ataatcgcct tcgtcggtgc caggccgaaa
     3121 cttcacttcc ttgacctgaa tgaccttctg cttggcccga gcctcggctt gacgcttttg
     3181 ctcttgatat ttgaatttgc cgtaatccat caatcgacag actggcggct cggcggtggg
     3241 cgcaatttca accaggtcca cttcgttttg ttctgataaa tggatggcat cctgggtctt
     3301 tacaacaccg agctgctcgc catctacccc aataagtcgc acctcgaggg cgcggatgtc
     3361 accgttgata cggtgaggtt tttcggttgc gatgtctaaa actcctagag ttgatgaaaa
     3421 ttcttgtttg gcgtcaagct ggcccgacgt cgcgccgggt gtcgatatct tgttgcaagc
     3481 gatcggcgaa agcagccaaa ggcataaccc ccaagtcaac gttgccacga ctgcgcaccg
     3541 aaacagtacg ggcttcgcgt tctttgtcgc ctaccaccag aatatacggt attttccgca
     3601 agctgtgttc tctgatttta cgggtgatct tttccgcgcg caaatcggaa ttcactctga
     3661 acccttgcgc cttcaggctt tgggcgactt ctgccgcgta gtcggcggac ggatccgaaa
     3721 tacagcatac gacgacctgt tcgggagcca gccacggagg cattgctccc gcgtgatttt
     3781 cgatcaagat cccgataaaa cgctcgaacg agcccaaaat ggcacgatgc aacatgacag
     3841 gcgccttgcg ctggtcgttg gcgtcgacat attcagcccc caggcgttgc ggcatggaaa
     3901 aatcgacctg aatcgtgcca cactgccaat ggcggccaat ggcgtcctta agcgtgtact
     3961 cgatcttggg gccataaaac gccccctcgc caacagaaat ttcgtattcg caatgcgtac
     4021 gatccaggct ttccatcaat gccttttcag atttatccca tacttcatcc gcgccaatcc
     4081 gtttatcggg gcgcgtggct accttatata gtatgtgagt aaatccgaaa tcggcataaa
     4141 ccttttgcag caaggcggta aacgccgcgc attcgtcctg caactggtcc tcggtgcaga
     4201 agatgtggcc atcgtcctgt gtaaaaccgc gcacgcgcat cagaccatgt aaggagcccg
     4261 acggttcgtt gcgatggcac tggccaaact ccccataccg aatagggagt tcgcgatacg
     4321 aatgcaatcc ggcattgaag atctgaacat ggccggggca attcatgggt tttaatccgt
     4381 agacccgatt ttcagattcg gttgtaaata tgttctcggc gtaattgtcc cagtgccccg
     4441 tctttttcca cagcgacaaa tcaagaatct gcggcgcctt gacttcctga tagccatttt
     4501 cgcggtaaac ctggcgcatg tactgttcaa cctgctgcca caaagcccag cctctggcat
     4561 gccagaagac caggccaggc gcctcgtcct gaaagtgaaa caagtccagc tcgcgaccca
     4621 atttgcggtg atcgcgcttg tcggcctctt ccatcatcgt cagatacgcc gcctgatctt
     4681 ctttgctggc ccaggccgta ccgtaaatac gctgcagcat ctcgttattg ctgtcgccgc
     4741 gccaataagc gcccgccagc ttggtcagct tgaagacctt cagcttgccg gtggacggta
     4801 cgtgcggccc gcggcataaa tcgatgaact cgccctcgcg atacaggctg atagtctgat
     4861 cctgcggaat cgatccaata atttccgcct tatagtgctc gcctatccca ttgaaatatt
     4921 tcacggcatc gtcgcgcgac cattcttccc gcgtgatggt ttcatctttt tttgccagct
     4981 cgaccatctt gttttcgatg gccacaaggt cctcgggcgt aaaaggccgt ttatacgaaa
     5041 aatcgtaata aaagccgttt tcaatcacag ggccaatggt gacctgcgcg tcgggaaata
     5101 acagcttcac cgcataggca agaagatggg ccgtggaatg gcgaattaaa tccagcccat
     5161 cgggatcttt ggccgtgaca atggccaggc gcgcatcttg ctcgatgcga aagctggtgt
     5221 ccaccagcct ggatgcatcg ccgtttagcg ctacccggcc gccgagcgcc gcgcgcccca
     5281 ggccggctcc aatagaatga gcaacatcgc caaccgtcac gggagcggga tactcgcgct
     5341 gcgagctatc gggcaaagta attcgaacca taaaacgtcc tgacaaaaaa aacgcggcca
     5401 tctgccgcgt ttttgggatc cttgatcttg ctagttagcc aatgaacaat acgcggcagc
     5461 agtcagttag cctgagtcaa tgtccgcgcc gtgcttttgt tcataaatca aattaggtaa
     5521 tgtaatcgtt gctttagtgt aacaccgcta tagcaggaaa ttcatcacaa tccgcaaagc
     5581 ggggcaaaga aagcaatttt tcagccgggt ccgttgtgaa aaagctcatg aacttgggcg
     5641 cgatccattg cccgttcaca gcgccggcct tacacttgaa atgacgtcct ttcttttgac
     5701 aagacttggc caaacaagtt agaatcgccc ttctttgctt taggcggtta gctcagttgg
     5761 ttagagcgct acgttgacat cgtagaggtc gctggttcga acccagtact gcctaccatt
     5821 tccttacttg gccttacctg gctgatcgaa gcccgtcggg gcacttttac tgcggcaccc
     5881 atttattggc cagccgggcg tattcaccgc cggctttgct taagtgcagc cattgatcca
     5941 cgaacaattt gaagcgcatg tcgccggcag gcagcagata agccatttcg gcgtattgca
     6001 gcggtttgtc ggggttgacg gcgcacaacc tgggattcag cttgctttgc accttggcct
     6061 cggcggcctc ggtcacgaag acgtcggctc ggccttcggc gacctctttg aagataccaa
     6121 tattattttc gtgagtagtg atgcgcgcct tggacaaatt agtcttggcg aatttctcgt
     6181 tgctgccgcc cgggttgacg attacgcgga catcgggctt gttgatgtcg gcaatagtct
     6241 ggaatttttt gacgttggtg cacagagtga ttggggtctt gccattgatc atataggcgg
     6301 aactgaaata ggcttttttc tgccgatcga gcgataccga gatcccgcca gcgccgatgt
     6361 cgcatttgcc tgatgtgaag tcatccatca gcttggccca ggtggtcttg acaaacgcgg
     6421 gctttgctcc cagtgcgccg gccagggaag ccatcaagtc tacgtccagg ccctcgaact
     6481 gaccatctgc cttttggaaa ctgaatggct tgtaatcgcc aggactgcat acgcgcaaag
     6541 tatggctttc gagcacatgg ctcaaggtgt ccgacggggc ggcgctggtc tgagcgaaga
     6601 ccaacgcggg tgcaaccagc aaggttgatg ccaatacatg tttcatggtt cttccttcgt
     6661 gcttgagcgt ggagaggctt gttattatga ttgaaatcat gcctcgcagg gggattaccg
     6721 tgtaatgccc gataaaccca ggcgccgtta aagcggcgtc tggaacgaac gcacctgaaa
     6781 agaaaaagac catcacccga tttggggagc tttatgatca cacacccatt tggcaatacg
     6841 aatcgcaata tcgccgcggt cggccaaggc acctgggaca tcgacacagc gcaacgcgat
     6901 caggccatcg ccactttgcg ccgcggcctg gatctgggca tgagtcatat cgacacggcg
     6961 gaaatgtacg gttcgggcgc cgcggagata ctcttgggcg aagcaatcaa gggccgcaga
     7021 gacgaggtct tcctggtctc gaaggttctg ccaaacaatg cgtcgcgggc gggtacacgg
     7081 gcagcctgcg agcgatcgct caggcaactc cgcacagact gcctcgattg ttatttgctg
     7141 cactggcgcg ggtcctaccc tctcgaagaa acattcgctg ccttcgagga tttgcaacgg
     7201 gacggcaaaa tccgctcatt tggcgtaagc aatttcgacg tcgacgacct cgacgaagcc
     7261 ttccagctac ttggccccgg aaagctcgcc tgcaatcagg tgctgtatca ccttcaagaa
     7321 cgcgccattg agcattcggt tatcccctgg tgcgaacggc atggactggc tgtcgttgct
     7381 tacagcccgc tcggccacga taattttcct tcgccggaca cagcgtccgg acgggtgctc
     7441 ggcaaggtcg cgcgggcgca cggggccacg ccacaccaga tcgcactcgc tttcctcacg
     7501 ctcagaccat cgatattcgc aatccccaaa gcctccagcg tgacacacgt cgaagacaat
     7561 gcggcaagcg ctgacattaa tctttccggg gccgaagtca gactcattga cgaggcgttc
     7621 ccgcgcgggc ccaagccaag tagcctgccc atgatttgag ccgggcgccg tcagcaaact
     7681 ctttcaaggc cggggtggct gaaattccga ttcaccaaag cgacccttgc acacccgccg
     7741 gtcccatcgc gggaggcggc tcgatttgtg cttcgggagc tttctgctgg acgttccccg
     7801 atattgccgg cttgtcgcgt gccgccggtg ttttgttctg cagccagtct atgccgggct
     7861 ggccgttttc ttgcagccac gcattggctt tctgaaaatg tccgcagcca atgaatggcc
     7921 gaggcggccg tagcgccgac agtggcgaag ggtggttggc ctttagcgcc aatagaggtc
     7981 ctttagcata gctctcgcgc agcaatgctt gtttggattg cgcgtgcgac ccccacagca
     8041 ggaacacttt cgggcgcgcc tcgcgcagca cacaagccag aatagtgtcg gtgacatgct
     8101 cccaaccttg attggcatgg gaagcgggct tgcgggcctc gaccgtgagg gatgtgttca
     8161 acagcagcac gccctggcgc gcccagcgta tcaggctgtt tcgctgacgc acaaaggtgt
     8221 cctgatattc gcgcgcgagt tcggcgaata tgttgcgcaa gctcggcggc gcggggcagg
     8281 catcggggac tgaaaaggcc agcccttgtg cctgatttgg accatgatag ggatcctggc
     8341 ccagaatgac gacctgcacg tcttgcggct cgacttccag taacgcccga aatggacgaa
     8401 gcgggaaaat ttgcgtgtgc cgcgccaact ggtccgccaa gtgatgatcc aggcttagca
     8461 acacgtcttg tgtgtgcgcg tcgtcaaggg cgtgttgcca cgcttgcgac agtgtacgca
     8521 catgccccat caagtctcct tcgaaggcat tccggaccgg tgctgccgaa ttgctcgttt
     8581 ggcgatactc tgtgctcata ttggcatacc aaccgttcag gcaagatgtc caccggtgac
     8641 gcccgtctgg cgcgcctcgt ggtggtggtc ggccgccatg aacaaataca tggcaggcac
     8701 gacgaacagc gtaaacaatg taccgatcga caggcccgtg aaaatcacga ggcccatcgc
     8761 gtgccggccc gccgccccgg ctcccgaggc aaacaccagg ggactgacac ccagcaccat
     8821 ggcggcggtg gtcatgagga tcgggcgcaa acgcacggcg gcggcttgtt caagtgcatc
     8881 acgtttgttg tagcccgcgc gctgcagttc gttggcgaac tgcacgataa gaatcccgtg
     8941 cttgctgatc agccccatca acgtgaccaa tccgacctgg gtgtagatat taagcgaggc
     9001 aaagccaatg tttatgaaca acagcgcgcc gaacagcgcc atcggcaccg agacgagtat
     9061 caccaccggg tcgcgaaagc tttcgaactg tgccgccagt gccagaaata cgataatggt
     9121 ggcaaacaac aatgtataga cgaagccccc cgactcgtgc gcaaactggc gcgaagcccc
     9181 cgagtagtcg atcgaatagc cgctgggtgc ggcctgttgc gtagcctgac tcaaatagct
     9241 caatacatcg ccctctgacg cgccaggcac cgcgacgccc gagatggtcg ccgaattgag
     9301 ctgctggaag tgattgatcg actccggtgc cacgtgcgat tcaagataag ccacggtgga
     9361 agcctggatc agaccgccat tcggcgtatg caagtaataa tcaaggattt gcgacggatt
     9421 caggcgatcg acctgcaaca cctgcggaat cactctgtaa gatcgtcccg caatagaaaa
     9481 gtagttgaca tagccgccac ccagcgcggc cgtgagagca ttgccgacat ccgcctgcat
     9541 cagcccgagc gatgccactt tgtcgcgatc aacgaccagc gtactctccg gcttgtcgat
     9601 tttaaggtcg ctgtcgacga agaaaaacat gcctgaagca cgcgctttgg ccagcaccgc
     9661 ctgcgataca tcgttcaggt tctgaaatgg ctctgtggtg gtaatgacga actgcacggg
     9721 caaaccctgc gtcccgggca aaggtggaaa ctggaacgcc gccacgcgcg ctccggcgat
     9781 ggtgttccac ttggcctgca gctcctgctg caattgcgcc gcccccttct tgcgctcgtc
     9841 ccaaggcttg agcagcacgc cgccaatgcc cctgttgacg gcgggcacgc ccgtcaattg
     9901 aaacatctgc tggtactccg gaatgccctt ggcaatgtga aatacctggt ccgcataggt
     9961 gttcatctgc tggatcgttg cattgggcgg gccctggatt tgcgagagca caatgccctg
    10021 atcttcttgc ggggccagtt cggatttcga cgtcatgaac aagtatgccg tgccggccag
    10081 caagagcgcg cccatcacca ccaacaccac ccacgtatca agcaaactgt gcaacagccg
    10141 ctgatagccg cgatgcactc gctcgaactg ccgatcgatg taacgcgcga aacgacctga
    10201 ttcctgatca gcgcgaaaga agcgcgagca catcatcgga gagagggtca gcgcgatcac
    10261 tccggagacg gtcaccgcgc ccgccagcgt aaatgcaaac tcggtaaaca acgcgccggt
    10321 cagcccgccc tgaaagccaa tgggcacata cacggccacc agcaccacag tcattgccag
    10381 gatcggaccg cccaactcgc gggctgccag tagcgccgcc tgaagcggtg gcttgccctc
    10441 ttccttcatg tgccgatcga cattctccac cacgatgatg gcgtcatcca ccaccaaccc
    10501 tatagccagc accagcgcaa gcaatgtgag cagattgatc gaatagccca gcgtcagcat
    10561 cacgaagaat gcgccgatca acgataacgg catggctatt accggaatga tcactgcacg
    10621 aaaactgccc agaaacagaa aaatcaccac agtaacgatg actagcgctt caatcagcgt
    10681 cttgaccact tcgtttattg atgtattgac gaagtctgtg gcgtcgtaca cgatcttgcc
    10741 cgtcaggcca gtgggcaatt gcgactgaac cgcgggaaat gcgtccctta cacgcttggc
    10801 cacgtccagc acattggctt ccggcgccgt tttgatacca atgaacaccg aacgccggcc
    10861 gctaaaagcc acgctgaagt catagttgtc cgctccaagc acaacatcgg cgacgtcctg
    10921 cagacgcacc agtgccccac tgtgcgactt gaccaccagt tgcttgaatt catcgaccga
    10981 atgcagatcc gtattggccg tcatgtcgac acttaccatc tggcctttgg tggctcccaa
    11041 cgccgccagg gtattgttat tgccaagagc cgcatagacg tcagcggcag tgacgccgtg
    11101 cgccgccatg cgc
//
LOCUS       DATCXU010000208         7223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1024662,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000208 DATCXU010000000
VERSION     DATCXU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024662"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1215)
                     /gene="glp"
                     /locus_tag="VIR04_04685"
     CDS             complement(10..1215)
                     /gene="glp"
                     /locus_tag="VIR04_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY9460075.1"
                     /translation="MLDFDIAQSRLADAGKRPTTTETCPLYRAAGRVLAQSVIATLDL
                     PPADNSAMDGYAIRYADYQAGRQFPVQQRCFAGMQPEPLQEGRAIRLFTGSLMPKGAD
                     TVIMQEDCDEVDDMLSIRTNPLSGAHVRKQGGDVHKGDVLLANGTQLSAAHIALLASQ
                     GLVDLPVYAQLRVGILTTGDELTFPGQPRANEQVFNSNGPMLAVLVDNLGARVVHVLH
                     ALDTRASLQKAFATLLRDCDLIITVGGVSVGEKDLVKPAIEQLGGTLDLWRVCMKPGK
                     PVALAHAKNRPIICLPGNPVSAFTVFAVLVSPMVRAMQGRAEIMPLIQSGRLKTVNEF
                     RETREEFLRVRANPTAQDLLELVPYDKQGSGMISSLPWAGGFARIRANTPTRDGDIVP
                     YYDYRHWLE"
     gene            complement(1218..1892)
                     /locus_tag="VIR04_04690"
     CDS             complement(1218..1892)
                     /locus_tag="VIR04_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY9460076.1"
                     /translation="MLLKDSLQSAAPWFNVLTVEQRERVLHDLTVARFPAGALVERKG
                     EKASGWTGVVNGLIKISVSNADGKMASLSGVPSGGWIGEGTLLKREVRKYDVVALRET
                     TIARLPAATFYWLLDNSITFNRYLLHQLNERVAQFIGKAEYDRLLAPDARVARCLAEL
                     FNPLLYPDKGARLAITQEELGYLARISRQRANQALQTLEAAHLLKVDYGAITVLDLEG
                     LKHYGI"
     gene            1996..3969
                     /locus_tag="VIR04_04695"
     CDS             1996..3969
                     /locus_tag="VIR04_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9460077.1"
                     /translation="MSHDRATQDSFELNTFPALVLHHARVRGNRPALREKDLGIWQIL
                     SWSELARQMRELANGFAALGIKPGEHIAVVGENRPRLYLSMMAAQAIGAIPVPLYQDA
                     IADEMQFVLQDAEIRLAVVEDQEQVDKMLEVGQRCPALNHVVYDDPRGLRNYVDDDLL
                     SFDRLLQLGRDFAQQHPGFFDAAVAAINTDDAAAMFYTSGTTGKPKGVVLTHHALIDR
                     ARAVEELENLTDREDVLAYLPPAWIGQNMFSYTQFLVTGFTVNHPESPETVAIDMRDI
                     GPTYYFAPPRVLEGLLTHVMIRMEDASALKRKVFHSCMSLAHRVGVRILDKDPGVSAW
                     DRFKYRIGDFMVYGPLRNVLGMSRVRVAYTAGEAIGPDLFVFYRSIGINLKQLYGSTE
                     TSVFVCVQPDGQVRSDTVGPPVKGVEIRVVENGEIQIKSPGMFKGYFRNPQATAQSYT
                     DDGWYHTGDAGYLDADGQLKIIDRAKDVGKMADGTLFAPKYIENKLKFFQYIKEAVAF
                     GADRDQVCVFINIDLEAVGNWAERSGLPYAGYTDLASKPRVYELIRDCINQVNAELAC
                     DPKLFGSQISRFLILHKELDPDDDELTRTRKVRRAFIARKYAVLVDALFEGKANQFIE
                     TEVKFEDGRIGRISADLKIATAQIFPGNTKKAA"
     gene            3972..4769
                     /locus_tag="VIR04_04700"
     CDS             3972..4769
                     /locus_tag="VIR04_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460078.1"
                     /translation="MIQATHGSQIGGTVLDLKNISLSFGGVKALTDISFNVREHEIRA
                     IIGPNGAGKSSLLNVINGVYTPQQGQITLDGVRFTRMDSRRAAEMGIARTFQNLALFK
                     GMSVLDNIMTGRNLRMTSGIFAQAFHLGSAQREEIANREFVENIIDFLEIQAFRKTPV
                     GRLPYGLQKRVDLGRALAMEPRMLLLDEPMAGMNIEEKQDMSRFILDVNDEFGTTIVL
                     IEHDMGVVMDISDRVVVLDYGKKIGDGAPDDVRSNPDVITAYLGAAH"
     gene            4785..5714
                     /locus_tag="VIR04_04705"
     CDS             4785..5714
                     /locus_tag="VIR04_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460079.1"
                     /translation="MGFFLETLFGGLMTGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     VAALSMARLSEWLPKWFGFESLLLANLLAFAISAVLMWLLALAIERWVLRYLVNQEET
                     TLLMATIGISYFIDGLGQIVFGSTVYSINIGMPKDPAFIFNSVFEGGVLISLEDLMAA
                     ALAALLVAALAFFFQYTSTGRALRAVADDHQAAQSIGIPLNRIWVIVWTVAGLVALVA
                     GMIWGSKFGVQFTLSTVALRALPVVILGGLTSVPGAIIGGLIIGVGEKLSEVYLGPFV
                     GGGIEIWFAYVLALVFLLFRPQGLFGEKIIDRV"
     gene            5747..6817
                     /locus_tag="VIR04_04710"
     CDS             5747..6817
                     /locus_tag="VIR04_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460080.1"
                     /translation="MFYRENGQFKSSYRADQQIFPIRQDRLFMAGVLVLAFLLVPLFA
                     SPYFLQAILIPFLILSLAAIGLNILVGYCGQISIGSGAFMAVGAYAAWNFGVRNPGMP
                     LIFQILLGGVFATLIGVVFGVPSLRIRGLYLAVTTLAAQFFADWAFLRIPYLTNYSSS
                     GNVSVPSLTAFGLPVQSPAQKYLFVLIIVTVFALLAKNLVRGAIGRQWMAIRDMDVAA
                     AVIGIRPVYAKLTAFAVSSFVIGVAGALWAYIHLGSWEPLAFDLGRSFQLLFMVIIGG
                     LGSILGSFFGAAFIVLVPVALTYVPGWFGLPLAVDTAAHIEHMVFGALIVFFLIAEPH
                     GLARLWSIGKEKLRIWPFPHEV"
     gene            6918..>7223
                     /locus_tag="VIR04_04715"
     CDS             6918..>7223
                     /locus_tag="VIR04_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460081.1"
                     /translation="MKRSFKLAVAMFVAAGTLGTMSTPAAAADEQFVPLLVYRTGSFA
                     PLGIPWADGKMDYLKLVNARDGGINGVKVTYEECETAYATDRGVECYERLKAKNGGAS
                     "
BASE COUNT         1457 a         1876 c         2123 g         1767 t
ORIGIN      
        1 ccgccagatt tattccagcc agtgacggta gtcgtaatag ggcacgatat cgccgtcccg
       61 tgtcggggtg ttcgcgcgaa ttcgggcgaa cccgccggcc cagggcaggg aactgatcat
      121 gcctgatcct tgcttgtcat aaggaacaag ctccaacaaa tcttgcgccg tcggattggc
      181 gcgcacgcgt aaaaattctt cgcgcgtttc ccggaactcg ttcacggttt tcaaccgtcc
      241 cgactggata agcggcatga tttcagcacg gccctgcatg gcgcgcacca tcgggctgac
      301 caggacggca aacacggtaa acgccgaaac cgggttgccc ggcaaacata tgatgggtct
      361 gtttttggcg tgcgccagcg ctacaggctt gcccggtttc atgcagacgc gccacagatc
      421 cagtgtgccg ccgagctgtt caatggctgg cttgaccaga tccttttcgc cgaccgagac
      481 gccgcccacg gttatgatca gatcgcagtc cctgagcaaa gtggcaaagg ccttttgcaa
      541 cgacgcgcgc gtgtccagtg cgtgcagcac atgcactacc cgggcgccca ggttgtcgac
      601 caaaaccgcc agcatgggcc cgttggagtt aaacacctgc tcgttcgcgc ggggctggcc
      661 ggggaaggtc aattcgtcgc ccgttgtgag gatgccaact ctaagttgag cgtaaaccgg
      721 caaatcgacg aggccttgcg aggccagcag ggcgatgtgg gctgcgctga gttgggttcc
      781 gttggccagc aggacatccc ctttgtgcac gtcgccacct tgcttgcgca catgtgcgcc
      841 gcttagcggg ttcgtccgaa tgctcagcat gtcgtcgact tcgtcgcagt cctcttgcat
      901 gatgacggtg tcggcgcctt tgggcatcag actgccagtg aaaaggcgta tggcccgccc
      961 ttcttgcaag ggttccggct gcatgccggc aaagcagcgc tgttgcaccg ggaactgccg
     1021 gcctgcctgg taatcggcat agcggatagc gtaaccatcc atggcgctgt tatcggccgg
     1081 cggcagatca agcgtggcaa taacagattg ggccaaaacg cgtccggccg cgcgatatag
     1141 cgggcaggtt tctgtcgtag tggggcgttt gccggcgtca gccagtcggg attgggcgat
     1201 atcgaaatca agcatgatta aattccatag tgtttcaggc cttccagatc aagcactgtg
     1261 atggcaccgt aatcgacctt tagcaggtga gcggcttcca gcgtctgcag cgcctggttg
     1321 gcgcgctgtc ttgaaatccg cgccaagtag ccgagttctt cttgggtgat cgccaggcga
     1381 gcgcctttgt cagggtagag caaagggttg aagagttcag ccaggcaacg cgccacgcgc
     1441 gcgtcgggcg ccagaaggcg gtcatattcg gccttgccga taaactgcgc cacgcgctca
     1501 ttgagctggt gcagcaagta tcggttgaaa gtgatgctgt tatcgagcag ccaataaaaa
     1561 gtggcggccg gcaagcgtgc gatggttgtt tcgcgcagcg cgacgacatc atattttctt
     1621 acctcgcgtt tcaggagtgt tccctcgccg atccatccgc cgctgggcac cccacttagt
     1681 gatgccattt tcccatcagc gttgctgacc gaaattttga tcaatccatt cacaacaccg
     1741 gtccagccgg acgccttttc accctttcgc tcaaccaggg cgccggccgg aaaacgagcc
     1801 acggtcaggt cgtgtaaaac gcgttcccgt tgttcgactg tcagtacgtt gaaccagggg
     1861 gccgcgcttt gcagagagtc tttcaatagc atcaggattt accctaggaa aggcgcgaat
     1921 gtcatattcg tgacagttca gtgtaatgca agcttatacc ctgcaaatag tgaaatatga
     1981 accgaagagg gcattgtgtc gcacgaccgg gcaactcaag attctttcga actaaacact
     2041 tttcccgcgc tggtgttgca ccatgcgcga gtgcgtggca atcggccggc attgcgcgaa
     2101 aaagacctcg ggatctggca gatattaagc tggagcgagc ttgcccgcca gatgcgggag
     2161 cttgccaacg gttttgccgc gctggggatc aaacccgggg aacacattgc tgtcgtgggc
     2221 gaaaatcggc cccgtctgta tttgtccatg atggcggcgc aggccattgg cgccatacca
     2281 gtgcctttgt atcaggatgc gatcgcggac gaaatgcaat tcgtgttgca agacgccgaa
     2341 atccggctgg ccgtggtcga ggatcaggaa caggtcgaca aaatgctgga agtcggccag
     2401 cgatgtccgg cgttgaacca cgtggtgtat gacgacccgc gcggtttgcg caactatgtc
     2461 gacgatgatc tgttgtcgtt tgatcgactt ttgcaattgg ggcgggattt cgcccagcaa
     2521 catccgggct ttttcgacgc ggcggtggcc gcaataaata ccgacgatgc ggccgccatg
     2581 ttttatacat cgggcaccac aggcaagccc aaaggggttg ttctgaccca tcacgcgttg
     2641 atcgatcggg cgcgcgctgt cgaagagctc gaaaatctca ccgaccgtga agatgttctg
     2701 gcttatttgc cgccagcctg gataggtcag aacatgtttt cgtacaccca gtttttggtc
     2761 accggcttca ccgtcaacca cccggaatct cccgaaaccg tcgcaatcga tatgcgcgac
     2821 atcggcccaa cgtattactt tgcgccgccc agagtgctgg aaggtctgtt gacgcacgta
     2881 atgatccgta tggaggatgc cagcgcattg aagcgcaagg tctttcattc gtgcatgagt
     2941 cttgcgcatc gcgttggtgt gcgcatcctt gataaggatc ccggcgtcag cgcatgggat
     3001 cgcttcaaat accggatcgg tgatttcatg gtttatggcc ctttgcgaaa tgtcctgggc
     3061 atgagccgcg tccgcgtcgc ctataccgcg ggcgaagcca ttggcccgga tctgtttgtg
     3121 ttttatcgtt cgataggcat caacctgaaa cagttatacg gatcgacgga aacttcggta
     3181 tttgtgtgtg tgcagcctga tgggcaggtg cgttccgata cggttggccc tccggtcaaa
     3241 ggtgtggaaa tcagggtcgt ggaaaacggc gaaatacaga tcaaaagccc tggcatgttc
     3301 aagggatatt ttcgcaaccc gcaggctaca gcccagtcgt ataccgacga tggctggtat
     3361 cacacgggcg acgccggata cctggatgcc gacggccaac tcaagatcat cgatcgcgcc
     3421 aaggatgtgg gaaaaatggc cgacggcaca ctgtttgcgc ccaaatatat agaaaacaag
     3481 cttaagtttt ttcaatacat caaggaagcc gttgcgtttg gagccgacag ggaccaagta
     3541 tgcgttttta tcaatataga cctggaggca gtcggcaact gggctgaacg atccggcctg
     3601 ccttatgccg gctataccga cctggcgtcc aagccccggg tctatgagct aatacgggac
     3661 tgcattaacc aggtgaatgc cgaactggcg tgcgacccca agctgttcgg ttcgcaaata
     3721 tcccgcttct tgattttgca caaagagctc gatcccgatg acgacgagct gacgcgtacg
     3781 cgcaaagtgc ggcgcgcgtt cattgcgcgg aagtacgccg tgctggtcga tgccttgttc
     3841 gaaggcaagg cgaatcagtt tatagagacc gaggtcaagt tcgaggatgg acgcattggc
     3901 agaatctcgg ctgatctcaa aatagcgacc gctcagatat ttcctggcaa caccaaaaag
     3961 gccgcgtaac aatgattcaa gcgacgcacg gcagccaaat tggcggcacg gttctcgatt
     4021 tgaaaaatat ctcgctgtca ttcggcggag tcaaagcgct tacggacatt tcttttaatg
     4081 tcagggagca tgaaatccgg gccattatcg gccccaacgg cgcgggtaaa agttctctgc
     4141 ttaacgtaat caacggtgta tacacgccgc agcaaggcca aattactttg gacggagtgc
     4201 gtttcaccag aatggattcg cgtcgcgccg ccgaaatggg cattgcgcgc acgtttcaga
     4261 atttggcgct gttcaaaggc atgagcgtgc tggataacat catgaccggt cgcaacctgc
     4321 gcatgaccag tggcattttc gcccaagcct ttcatctggg atcggcccag cgtgaagaaa
     4381 tcgccaaccg tgaattcgtc gaaaatatca tcgattttct cgagattcag gcgtttcgaa
     4441 aaactcccgt gggccgcttg ccttacggcc tgcagaagcg cgtcgaccta gggcgcgcgc
     4501 tggcgatgga gccgcgcatg ctgctgctgg atgagcccat ggctggaatg aatattgaag
     4561 aaaagcagga catgagccgc ttcatactcg atgtaaacga cgaattcggc accacgatcg
     4621 tattaattga acatgatatg ggcgtggtca tggatatttc cgaccgggtg gtggttcttg
     4681 attatggcaa aaagatcggc gatggggcgc ccgacgatgt tcgttccaat ccagacgtta
     4741 ttaccgccta tcttggcgcg gctcattagg caagcgagat aaccatggga ttttttctgg
     4801 aaactctgtt cggcggcctg atgacgggca tgctttattc tctggtcgcc ctgggctttg
     4861 tcctgatctt caaagcgtcg ggagttttca attttgcgca aggggcgatg gtgttggtgg
     4921 cggcgctgtc catggcgcgc ttgtccgaat ggctacctaa atggtttggg tttgaaagcc
     4981 tgttgttggc gaacctgctg gcctttgcga tcagcgccgt tctgatgtgg ttgctggcac
     5041 ttgcaatcga gcgctgggtg ctgcgctatt tggtcaatca ggaggagacg acgttactga
     5101 tggccaccat cggcattagc tattttatcg atggcctggg ccagattgta ttcggcagca
     5161 cggtttattc catcaatatt ggcatgccga aggatccggc atttatattc aattcggtgt
     5221 ttgaaggcgg ggtgctgatc agtcttgaag atttgatggc agccgctctg gccgcgcttc
     5281 tggttgcggc actagctttc ttttttcaat atacgtcgac cggccgggcg ttgcgcgcgg
     5341 tggccgatga tcaccaggcg gctcagtcaa ttggtattcc actgaaccgg atatgggtca
     5401 ttgtgtggac cgtcgcaggt ctggttgccc tggtggcagg catgatatgg ggctcgaaat
     5461 tcggagtgca attcacttta tccacagtcg ctctgcgggc gctgccggta gtcattctag
     5521 gaggtctcac atcggtgccc ggagccatca tcggcggatt gattattggc gtgggtgaga
     5581 agctatccga ggtttatctg gggcctttcg taggcggggg cattgaaatc tggttcgcct
     5641 acgtgctggc attagtgttt ttgctatttc gcccccaagg actattcggg gaaaaaatca
     5701 tcgatcgtgt ttgatggttc tttgaagaaa atcaggatac tgcactatgt tttatcgcga
     5761 aaacggtcag tttaagagca gctatcgcgc tgaccagcag attttcccca tacgccagga
     5821 tcgactcttc atggcgggtg tgctggttct tgcttttctg cttgtgccgc tgtttgcttc
     5881 gccttatttc ctgcaggcga tcctgattcc gtttctgatt ttgtcgctgg ccgcgatagg
     5941 cctgaatatc ctggtgggtt attgcggcca aatttccata ggatccggcg ctttcatggc
     6001 ggttggagct tatgcggcct ggaatttcgg tgtgcgcaat cccggaatgc cgctgatctt
     6061 tcaaattttg ctgggcggcg tgtttgcaac attgattggc gtggtgttcg gtgtccccag
     6121 tttgcgcatt cgcggcctgt atttggccgt cacgacgctg gcggcgcagt tttttgcgga
     6181 ctgggcgttt ttgcggattc cctatctgac caattattca tcttcgggca atgtatcggt
     6241 gccttcgctc acggctttcg gcttgccggt ccagtcgccc gcgcaaaaat atttgttcgt
     6301 gctcattatt gtgactgtgt tcgctctgct ggctaaaaac ctggtacgcg gcgccattgg
     6361 acgtcagtgg atggccatcc gcgatatgga cgtggccgcg gcggttattg gcatccggcc
     6421 tgtgtatgcc aagctgaccg catttgcggt tagttctttc gttattggcg tggctggcgc
     6481 cttgtgggcc tacattcatt tgggctcatg ggagcccctg gcgttcgact tgggacgttc
     6541 gttccaattg ctgttcatgg tcattattgg gggactgggc tcaatcctgg gcagcttctt
     6601 cggtgcggct tttattgtgt tggtgccggt cgcgctcacc tatgtgcccg gttggttcgg
     6661 cttgccgctc gctgtggata ctgcggctca tatcgagcat atggtgtttg gcgcgttgat
     6721 tgtgtttttc ctgattgccg aaccgcatgg cctggcgcgg ttgtggagca ttggcaaaga
     6781 gaagttgcgg atctggccgt tcccgcatga ggtgtgatgg ccggttgatt ttgtcggctt
     6841 tttaaacgat cggctgtctc tcctgagcgt agccggtcat aaaacggtgt aactagcacc
     6901 atacggaggt tctggaaatg aaacgttcat tcaagcttgc cgtcgccatg tttgttgccg
     6961 cgggcaccct gggcacaatg tccacgcctg cagccgcagc agacgagcaa tttgttcccc
     7021 tgttggttta tcgcaccggc tcatttgctc ctctgggaat tccatgggcc gatggaaaaa
     7081 tggattacct gaagctggtg aatgcccgcg atggcggtat taatggcgtc aaggtcacat
     7141 acgaggaatg tgaaaccgcc tatgcaaccg atcgcggcgt tgaatgctac gagcgcctga
     7201 aggccaagaa cggcggagcc tcc
//
LOCUS       DATCXU010000209         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_608702,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000209 DATCXU010000000
VERSION     DATCXU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608702"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1456)
                     /gene="glmM"
                     /locus_tag="VIR04_04720"
     CDS             complement(110..1456)
                     /gene="glmM"
                     /locus_tag="VIR04_04720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY9460082.1"
                     /translation="MARRKYFGTDGVRGEVGGATINAGVALRLGYAAGTVLARKNPGR
                     GRPRVIIGKDTRISGYMLESALEAGFSAAGVDVLLAGPIPTPAVAYLTRALRLVAGIV
                     ISASHNPYQDNGIKFFSAQGMKLPDELEAEIETAIDQPLGCVRSEDLGRARRIDDASG
                     RYIEFCKSTFPNELDLSGMKIVVDAAHGAAYYVAPHVFRELGAEVYSIGCQPDGLNIN
                     KNVGALYPDALIAEVKARGANVGIALDGDADRLQMVDASGRLYNGDELLYAIVRDRMT
                     RHHVDGVVGTLMTNFGFEKKMLSLGVGFDRSNVGDRYVLERMQSRGWLYGGESSGHLI
                     CLDCHTTGDGIIAALQVLKGMSRSGETLAQRVADLKMYPQIMVNVPLEPGIQWEHHAG
                     LMAAKTSVEQRLNGRGRVLIRASGTEPKLRLMVEADDAALAEQSVEQLRAVDLTAN"
     gene            complement(1456..>2079)
                     /gene="folP"
                     /locus_tag="VIR04_04725"
     CDS             complement(1456..>2079)
                     /gene="folP"
                     /locus_tag="VIR04_04725"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY9460083.1"
                     /translation="SAADELQRVLPVIDALREAGVPLSVDTFKPEVMRRALDVGVDMV
                     NDIYGFRQPGAIEAVTDSRCGLCVMHMRGEPRTMQLAPQYKDLVNDIRQFLETRVNIM
                     VQSGIGPQRLVLDPGFGFGKTTEQNYSLLRHLDQLQIEQYPLLLGLSRKSMIGHVTGR
                     AASERLAGSIAAALAGVARGARILRVHDVAPTVDALKVWRAVELGVS"
BASE COUNT          460 a          682 c          532 g          405 t
ORIGIN      
        1 ttgccaccca ttcattgtcc gaatacagtt tgcgcggcga aatcacgcag tggggcaagg
       61 ccggcttttc ctgccgtatg atcgaacgtt agcgcgatcg tgcgtggcgt cagttcgcag
      121 tcaaatccac ggcacgcaat tgctcgacac tctgttcggc caacgcggca tcgtcggctt
      181 cgaccattaa tcgcagtttg ggctcggtgc ccgaggcgcg aatcagcacc cggccgcggc
      241 cattcaagcg ttgctcaacg gacgtcttgg cggccatcag tcccgcatga tgctcccact
      301 gtatacccgg ttcaagcgga acattgacca tgatctgtgg atacatttta aggtcggcga
      361 cccgctgcgc cagcgtttcg ccactgcggc tcattccttt caggacttgc agggcggcaa
      421 taattccgtc gcccgttgta tggcaatcaa gacaaatcaa atgcccagag ctttcccccc
      481 cgtacagcca gccgcgggat tgcatccgtt ccagcacata gcggtcgccc acattactgc
      541 gatcaaagcc gactcccaag gagagcatct tcttttcaaa gccgaagttg gtcatgagcg
      601 tgccgaccac gccgtcaaca tgatgccgcg tcatgcggtc gcgcacgatg gcgtaaagca
      661 gttcgtcgcc gttataaagt cgcccggaag catcgaccat ttgcaaacga tcggcgtcgc
      721 catccagggc aatgcccaca ttcgcgccgc gcgccttaac ttcggcgatt aaagcatcgg
      781 ggtacagcgc cccgacattt ttattgatgt taagcccatc gggctggcat ccgatcgagt
      841 acacctcggc gccaagctcg cgaaacacat ggggcgccac atagtacgcc gccccatggg
      901 cggcatcgac cacgatcttc attcctgaca aatcgagttc attgggaaac gtgcttttgc
      961 aaaattcgat gtaacggccc gaagcatcgt caatgcggcg agcgcggccc aggtcctcgg
     1021 atcgcacgca ccccaaaggc tgatcgatcg cggtctcgat ttcagcctcg agttcgtcgg
     1081 gcaatttcat cccctgcgcc gagaaaaatt taatgccatt gtcctgatac ggattgtgcg
     1141 atgcgctaat gacaatgccg gcaaccaggc gcaacgcgcg cgtcagataa gcaaccgccg
     1201 gggtcggtat cggtccggcc agcaaaacat caacgcccgc cgccgagaag ccggcttcca
     1261 gagccgattc cagcatataa ccggatatgc gcgtgtcctt gccaatgatc accctggggc
     1321 ggccgcgccc cggattcttg cgcgccagca ccgttccggc cgcataaccc aaacgcaacg
     1381 cgacccccgc attgatcgtc gcgccgccca cttcgccgcg caccccatcc gtaccgaaat
     1441 atttccgccg agccatcaag acactccaag ttccaccgcg cgccacactt tcagcgcatc
     1501 gaccgtaggc gcgacatcgt gcacgcgcaa aatacgggct ccgcgcgcca cccccgccaa
     1561 cgcagcggca atactgccag ctagacgctc cgacgcggcg cggcccgtta catgacctat
     1621 catcgatttg cgcgacagtc ccaacaataa cggatattgc tcgatctgca actggtccaa
     1681 atgacgcaac aacgaataat tctgctcggt cgttttgcca aatccaaagc ctggatccag
     1741 aacgagccgt tgcgggccga tgccgctttg caccatgata ttcaccctgg tttccaggaa
     1801 ttgccgaata tcgttcacca aatctttgta ttgcggcgcc aattgcatgg tgcgcggctc
     1861 gccgcgcata tgcatgacgc acaagccgca acgggaatcc gtcaccgctt caatcgcccc
     1921 tggctggcga aaaccataaa tatcattaac catgtcaacc ccgacatcca gcgcacggcg
     1981 cattacctcg ggtttgaatg tgtcgaccga caagggaacg cccgcttcgc gcaacgcgtc
     2041 gataaccggc aaaacgcgtt gaagctcatc ggccgccga
//
LOCUS       DATCXU010000210         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_472803,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000210 DATCXU010000000
VERSION     DATCXU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472803"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VIR04_04730"
     CDS             complement(<1..495)
                     /locus_tag="VIR04_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460084.1"
                     /translation="MEEHGKVLANSHQISDSLAHDQLLARLANNESVLSNACRLLRDA
                     TKIGRQITPAAEWLLDNFYLIEEQIRTAKLHLPKGYSKELPRLSKGSLAGYPRVYDIA
                     LEIISHGDGRVAPEGLSRFVAAYQQVTVLKLGELWAIPIMLRLALIENLRRVAARLAA
                     SRHHR"
     gene            complement(840..1817)
                     /locus_tag="VIR04_04735"
     CDS             complement(840..1817)
                     /locus_tag="VIR04_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEY9460085.1"
                     /translation="MQKIHVIHENSAWVEPLRAAFAELDLPYEEWFLDEGMLDLTDPP
                     PQGVFYNRMSASSHTRDHRFGPEYTAGVLAWLESHDRRVINNSRALQLEVSKIAQYAA
                     LSKLGIRTPHTIAAIGQKNIMAAAQKMKGAFITKHNRAGKGLGVHLFHTIEALKSYVE
                     GPDFEDSVDGITLIQEYIRAPEPHITRVEFVGGQFLYAVRVDTSLGFELCPADACQIG
                     DAFCPVGENAASPAPAAPRFQIIDGFNDAIIERYRRFIANNGIGIAGIEFIRDADGQI
                     YTYDVNTNTNYNSAAEAQAGLFGMRSIAKYLGDELYKLGDARHVLSAAA"
     gene            complement(2010..>3189)
                     /locus_tag="VIR04_04740"
     CDS             complement(2010..>3189)
                     /locus_tag="VIR04_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY9460086.1"
                     /translation="GWQAEIAAIRRDIHAHPELAYQENRTSDLIAKQLASWGIEVHRG
                     LGVTGVVGTITGRKSNGRSIGLRADIDALPMQELNTFEHASTHPGKMHACGHDGHTAM
                     LLAAARYLAQHRDFDGSVHVIFQPAEEGFVGAKKMIEDGLFKLFPVDAVFGMHNWPGM
                     EVGRFGVITGPVMASSNYFKITIKGKGAHAAMPHLGVDPIMAAVQLAQSFQTILTRNR
                     NPLDPAVLSLTQLHAGSADNVIPNNAEMRGTVRTFSEATLDMIETRLRELTQHTCRAF
                     NCEASFSFDRKYPPTINHANETAFAASVLGDIVGEENVDRTLSPSMGGEDFSLMLKEK
                     PGCYVWIGNGQGDHRLPGHGLGPCMLHNASYDFNDELIPLGASYWAELARRWLAQPAN
                     "
BASE COUNT          622 a          846 c          988 g          733 t
ORIGIN      
        1 ccgatgatgc cggctggcgg ccagacgggc ggcaacgcgg cgcaaattct cgatcaacgc
       61 caggcgcagc atgatgggga tggcccacag ttcgcccaat ttcagtacgg tcacttgctg
      121 gtatgcagcg acgaagcggc ttaatccttc gggcgcgacc ctgccgtcgc catgcgaaat
      181 gatttcaagc gcgatgtcgt aaacgcgtgg atagcctgcc agggatcctt ttgacaagcg
      241 cggcaattct ttgctgtagc ctttgggcaa atgcagctta gccgtgcgga tttgctcttc
      301 aatcagatag aaattgtcga gcagccattc cgcggcaggc gtgatttgac ggcctatctt
      361 ggtggcgtcg cgcagtaacc ggcatgcatt ggacagcacg gactcgttgt tggccagccg
      421 ggccagcaat tgatcgtgag cgaggctgtc gcttatttga tgcgaattcg ccagaacttt
      481 cccatgctct tccatctgag ccgcgctgaa cagctcggct ctgagcggca attcttcatt
      541 gatggaaagt ctgggcggcc ggctactgaa ttgcgcttct gcccagctct tgaaactcga
      601 gaagatggac agttggttta tattcaatgc acacgcccct tttattgttt agcgcaatct
      661 acggcctgcg ccggataggc cggacgcgat aagcctgccg cctacaagtg tacagtgtgg
      721 ttattttcgg cgtggcgcag gggtcgtagc cgaatgtgtt ttagtgtaac ggggcgttgg
      781 gggcggcgcc gctggcgcag ccagggcgat tacacgaagg tcggacgaga gcgcgaaaat
      841 caggcggccg cggacagtac atggcgggca tcacccagtt tgtacaactc atcgccaaga
      901 tattttgcga tggagcgcat tccgaacaag ccggcttggg cttcggcggc actgttgtag
      961 ttcgtattgg tgttgacgtc gtaggtgtaa atctggccgt cggcgtccct gatgaattca
     1021 ataccggcga tgccgatccc gttatttgcg atgaagcggc ggtagcgctc gataatggca
     1081 tcattgaaac cgtcaatgat ctgaaaacga ggagctgcgg gcgccgggct tgcggcgttt
     1141 tctcccacag gacagaacgc gtcgcctatc tggcaggcat cggcggggca aagttcgaat
     1201 ccaagcgagg tgtcgacccg cacggcatac aggaactggc cacccacgaa ctcaacccgc
     1261 gtgatgtgag gttccggcgc ccggatgtat tcctggatga gtgttatgcc gtcgacggag
     1321 tcctcaaagt cgggcccttc gacgtagctt ttaagggctt cgatcgtgtg aaacagatgc
     1381 acgcccaagc ctttgccggc ccgattgtgt ttggtgatga acgcgccttt cattttctga
     1441 gcggccgcca tgatgttttt ttgcccgatg gcggcaatgg tgtgaggtgt gcgaatgccc
     1501 agtttggaca gtgcggcgta ctgtgcaatc ttgctgacct cgagttgcag ggcgcgactg
     1561 ttgttgatga cgcggcgatc atgtgattca agccatgcca gcacgcccgc ggtgtattcg
     1621 gggccgaaac ggtgatcccg ggtgtgcgat gaagcgctca tccggttgta aaacacgccc
     1681 tgcggtggag gatcggtcag atcaagcatc ccttcgtcca gaaaccattc ctcgtaaggc
     1741 aggtcgagtt cggcgaatgc cgcacgtagc ggttcgaccc aggcggagtt ttcatggatg
     1801 acatggattt tttgcatgat ttcgtttcgg taagggcaac aggcaggcct gtctatcgtt
     1861 cgacctgttt ccccagcata atgaaggcgt gccatattga aaacgaatat atagacatat
     1921 tcttattcga aatgagcata tagaacgcgt gggcttttat tgcacggctg tggcgctggc
     1981 gtcagcactg gcgccgcggg gtgtgcgggt cagttcgccg gttgagccag ccagcgccgg
     2041 gccagttcgg cccagtaact ggctcccaaa ggaatcagct cgtcgttgaa gtcgtaactg
     2101 gcgttgtgca gcatgcaggg ccccagtccg tgccccggta aacgatggtc gccctgaccg
     2161 ttgccgatcc agacgtaaca gcccggtttt tccttgagca ttaaagaaaa gtcctcgccg
     2221 cccatggatg gtgaaagggt ccggtccacg ttttcttcgc ccacaatgtc gcctaagaca
     2281 ctcgcggcaa acgcggtttc gttggcgtga ttgatggtgg gggggtattt ccggtcgaac
     2341 gaaaagcttg cttcgcaatt gaatgcgcgg caggtgtgct gcgtcagttc gcgcaagcgt
     2401 gtctcaatca tgtccagggt ggcttcggaa aatgtacgaa ccgtgccgcg catctccgca
     2461 ttattgggaa tgacattatc ggccgaaccg gcatgcaact gcgtcaggct taataccgcc
     2521 gggtccagtg gattgcggtt gcgtgtcagg atggtttgaa agctttgggc cagttgcacc
     2581 gccgccatga tcgggtcgac tcccaggtgg ggcatggcgg cgtgggcgcc cttgccttta
     2641 atggtgattt tgaagtagtt gctcgaggcc atgacaggcc cggtgatgac tccgaaacgg
     2701 ccgacctcca tgcctggcca gttatgcatg ccgaacacgg catccacggg aaacagtttg
     2761 aacaggccgt cttcgatcat ttttttggcg ccgacgaagc cttcttcggc gggctggaaa
     2821 ataacatgga cgctgccgtc gaagtcgcga tgctgcgcca gataacgggc cgcggccagc
     2881 agcatggccg tgtgtccgtc atggccgcac gcatgcattt tcccggggtg cgtgctggcg
     2941 tgttcgaaag tattgagttc ctgcatgggc agcgcgtcga tatcggcgcg caatccgatc
     3001 gatcgtccgt tggattttcg gccggtgatc gtgccaacca cgccggtaac gcccagtccg
     3061 cgatgcactt cgatgcccca ggacgcaagc tgtttcgcta tgagatcgga cgttcgattt
     3121 tcctggtagg cgagttccgg gtgggcgtgg atgtcgcggc gaatagccgc aatctcggct
     3181 tgccagccg
//
LOCUS       DATCXU010000211         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1113443,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000211 DATCXU010000000
VERSION     DATCXU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113443"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIR04_04745"
     CDS             complement(<1..182)
                     /locus_tag="VIR04_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460087.1"
                     /translation="MRCLQLKRWIVSALIPILGVAIPGVVWGAETSGQAVHSTRASHS
                     AKTTHSAKKAHPAKSKR"
     gene            complement(182..2563)
                     /locus_tag="VIR04_04750"
     CDS             complement(182..2563)
                     /locus_tag="VIR04_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY9460088.1"
                     /translation="MRLVWWIIFFAVACMSNAWAQPSGSGSSTSASLSGLQISPDLQI
                     HKTLNEDRMTVFLVADKMRTDAEGKIILTGSAEVRRIDSVVKGDYIDYQRSNGQMHVR
                     GNGLILRDASIVTGPELNYNVKSETGDVSNPDFWLGANGGAGTAVKADIFSKSKMRLS
                     DVVYSGCPCPEPSWYIKSPTVDLNFAENEGVARNGVLYFKGVPILASPYLSFPLRKER
                     KSGFLVPTYGVSSNSGFELSTPYYFNLAPNYDATLTPRYMSKRGTQLGGEFRYLGDTY
                     SGQVAGTYLSNDLQTRSNRWMYSAQHNQTLGNGFSASYDIRRVSDNDYFRDFSTFGLN
                     EATTNLLPSVAQLNWSGAEYWSSYLQTYTYQTLQDRTAGYTMPPFDKLPELYVRGARY
                     NWGGFDVISDNYATKFRMPHFSDDTHSYPFASQLSPNQSYDGERFMSYTTVAYPIQRA
                     GWYVTPKVGLHMTEYNTNWHGSGWQISPTPGPHSQSRILPIMSVDSGMTFERDTRLFG
                     NDSVQTLEPRLYYLYVPYTNQSLLPVYDTAVSTFNFSQAFDENRYSGGWDRISNANQL
                     TLGLTSRWLDADTGFERLSLSAAQRIYFTDQRVTLPGETPRTDNSSDYLVGANAALTD
                     KLSVRFDGQYNPKSGQRNQMTAGFRWQPKRLASVSVAYLYQRNPLQILNPALDSSVAP
                     SKDQVTLSGQWPIVNKLSAMGRVDYSLEEKRTTQSVLGLEYKGDCCWTARVVMQRYAV
                     SAQQVNTALFFQLELSGLGSLGTDPMSLLRRTISDYESTTPPIPEKTNFERYE"
     gene            2757..3734
                     /locus_tag="VIR04_04755"
     CDS             2757..3734
                     /locus_tag="VIR04_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY9460089.1"
                     /translation="MHKLELDSLQPASSDASFRRYFRVMGAGRSFIVMDAPPDHEDCG
                     PFLHVTRLLRQAGINVPAILAQDIPNGFLLISDLGHQTYYQAIQTGLSDQRLQTLYRD
                     AIRALVQMQHADTAGLPVYDRGRLMQELDLFPQWYVQRHCQARLDDNERSVLHKAFTT
                     LAEDNVKQAYVFVHRDFHSPNLMITDQSGGGPNPGIIDYQDALSGPLTYDVASLVMDA
                     RTTWEEPQQLDWAIRYWEAARKDGLPVPRDFAEFHRAYEWMSLQRNLRILGVFARLAH
                     RDHKDDYLQHMPRVNAYIRQVAGRYGVFRPLLRLLDKLDKRQELAGYTF"
     gene            3734..>4350
                     /locus_tag="VIR04_04760"
     CDS             3734..>4350
                     /locus_tag="VIR04_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY9460090.1"
                     /translation="MRAMILAAGRGQRMRPLTDTIPKPLLVAGGKPLIVWQVERLVRA
                     GITEIIINHAWLGMQIECTLQDGRQYGAAITYSAESPALETAGGIAKALDFFHGRPFL
                     VVNSDIWCDWDPVHARRLGQMLLNEQKLAWLLLADNPPHHPAGDFLLNAAGLVVDRAS
                     PEGGAPLTFTGIGIYQPALFENLNKKQPAPLAPLLRQAMRQNVVAG"
BASE COUNT         1001 a         1235 c         1181 g          933 t
ORIGIN      
        1 cgcttgcttt tggctggatg ggctttcttg gccgaatgtg tcgtcttggc tgaatggctg
       61 gccctggtcg aatgaacggc ctggccggat gtctccgcac cccagaccac gccgggaatt
      121 gccacgccca gaatcggaat cagagccgac acaatccagc gttttagttg caaacaacgc
      181 attattcata cctctcgaag ttggtttttt caggtatcgg cggcgtagtc gattcgtaat
      241 cggagatcgt tctgcgcaat aaagacatcg gatcggtgcc cagggaaccc aaacctgaca
      301 attccaattg gaaaaacaag gccgtattaa cttgctgagc cgacacggcg taccgctgca
      361 taaccacccg cgccgtccag caacaatcgc ctttatactc cagaccgaga accgattggg
      421 tggttcgctt ttcttccagc gaatagtcga cccgccccat ggctgaaagc ttgttgacta
      481 tgggccattg gccggatagc gttacctggt ctttgctggg tgcgaccgat gaatccaggg
      541 caggattaag tatctgcaac ggattgcgct ggtaaagata ggccaccgat acagaggcca
      601 gccgcttggg ctgccagcga aatcccgccg tcatctgatt gcgctggccc gacttcggat
      661 tatattgccc gtcaaaccgg acactaagct tgtcggtcaa cgccgcgttg gcgcccacca
      721 gatagtcgga ggagttgtcc gttcgcggcg tctctccggg cagtgtcacg cgctgatctg
      781 tgaaataaat ccgctgcgcc gctgagaggg acaaacgctc aaagcctgtg tccgcatcca
      841 gccagcggct tgtcaggccc aacgtaagct gattcgcatt ggagatacga tcccatccgc
      901 cactgtaccg gttctcgtcg aaagcctggg aaaaattgaa cgtggaaaca gccgtgtcgt
      961 aaaccggcaa aagcgattga ttggtgtagg gcacatacaa ataataaaga cgcggctcca
     1021 gagtctgcac cgaatcgttg ccgaacagcc tggtgtcgcg ttcgaacgtc atgcccgaat
     1081 cgaccgacat gatgggcaat attcgggatt ggctgtgcgg gcccggagtc ggggaaatct
     1141 gccagcccga accatgccag ttcgtgttgt attcggtcat gtgcaaaccc actttgggcg
     1201 tgacatacca acccgcgcgt tgtattggat acgccaccgt cgtatacgac atgaagcgct
     1261 cgccatcata ggactgattg ggggacaact gactggcgaa cgggtagctg tgggtatcat
     1321 cgctgaaatg gggcatccgg aacttggtgg catagttgtc cgaaattaca tcgaacccgc
     1381 cccagttata gcgggccccg cgtacataaa gctcaggcaa cttgtcgaag ggcggcatag
     1441 tgtaacccgc cgtcctgtcc tgcaatgtct ggtatgtata cgtttgcaga tacgagctcc
     1501 agtactcggc gccgctccag ttcaattgcg ccacgctggg caataaattc gtcgtcgcct
     1561 cgttcaggcc gaaggtcgag aaatcgcgaa aataatcgtt atccgagacg cggcgaatat
     1621 cgtacgaagc gctaaaaccg ttaccaaggg tctgattgtg ctgggccgaa tacatccagc
     1681 gattggatcg cgtctgaaga tcgttcgaca aataggtgcc cgccacctgc ccactgtacg
     1741 tatcgcccaa ataacgaaat tcaccgccca actgtgtacc gcgcttgctc atgtagcgcg
     1801 gtgtcagggt cgcatcgtaa ttgggagcca gattaaagta ataaggagtg gatagctcga
     1861 aaccgctgtt gctggataca ccataggtcg gaaccaggaa ccccgatttg cgttccttcc
     1921 tgagcgggaa cgacaagtac ggagatgcca gaatagggac gcctttaaag tacagaacac
     1981 cgttgcgggc gacaccctcg ttctcggcaa aattcaaatc aacagtgggc gatttgatgt
     2041 accacgaggg ttcggggcaa gggcaacccg aataaaccac atcggacaag cgcattttgc
     2101 ttttactgaa aatatcggct ttgaccgccg tgccggcccc gccattcgcg ccaagccaga
     2161 aatcggggtt gctcacgtcg cccgtttcac tcttgacgtt gtaattcagt tcgggcccgg
     2221 tgacaatgct ggcgtcgcgc aatataaggc cattgccgcg cacatgcatt tgcccattgg
     2281 agcgttgata gtcgatgtaa tcgcccttga cgaccgaatc gatgcgccga acctcggccg
     2341 atcccgtcaa aataattttt ccttcggcgt ccgttcgcat tttgtcggcc accaggaata
     2401 cggtcatccg atcttcattc agggtcttgt gaatttgcag gtcgggcgat atttgcagcc
     2461 ccgacaggga agcagacgtt gacgacccgg agccggacgg ctgggcccac gcattggaca
     2521 tgcaagcaac tgcaaagaag attatccacc aaaccaaacg cacaagtacc ctgtctttga
     2581 agatcggcta tattttggca cagttgccgc gccagaacga atagcaaccc ggtattatag
     2641 agagaagccg gcattcgcgc gggcaactct ataccaaccg ctacggaatt accgtgaacc
     2701 caggcactca accacgactc gcgttattaa acaactggct gcgttcgctg gcggccatgc
     2761 acaagctgga acttgacagc ctgcagcccg cttccagcga cgccagcttt cgccgctatt
     2821 tccgcgtaat gggagcagga cggtctttca ttgtgatgga tgccccgccc gatcatgaag
     2881 actgtggccc ttttcttcat gttacccggt tgctgaggca ggcgggcatc aatgtgccgg
     2941 cgatcctggc gcaagacatt ccgaacggct tcttgctgat ttcagatttg ggacatcaaa
     3001 cttactatca ggcgatacaa acaggcttga gcgaccagcg cctgcaaacg ctttatcgcg
     3061 acgcaatcag ggctttggtg caaatgcaac atgccgacac ggccggtttg cccgtctacg
     3121 accggggccg gctgatgcaa gagcttgatc tttttccgca atggtacgta caacgccatt
     3181 gtcaggccag acttgacgat aacgagcgct ccgtactgca taaggcattt accacgctgg
     3241 ccgaagacaa cgtaaaacaa gcgtacgtat tcgtacaccg ggattttcac tcacccaatc
     3301 tgatgataac cgatcaatcc ggaggcggcc ccaaccctgg aattatcgat tatcaggatg
     3361 cattgtccgg cccgttgacc tatgacgtcg catcgctggt gatggacgcc cgcaccacat
     3421 gggaagaacc acaacaactg gattgggcaa ttcggtattg ggaagccgcc cgaaaggacg
     3481 gtcttcccgt accacgtgat ttcgccgagt ttcatcgcgc gtacgaatgg atgagcctgc
     3541 aacgcaatct gcgcattctg ggagtcttcg cccggcttgc tcaccgcgat cacaaggacg
     3601 actacttgca gcatatgcct cgcgtgaacg cctatatccg acaggttgcg gggcgctacg
     3661 gggtgttccg gcccttgctg cgactgctcg acaaactcga taagcgtcaa gagcttgccg
     3721 gatacacgtt ttaatgcgcg caatgattct tgccgccgga cgcggccagc gcatgcgtcc
     3781 gttaacggat acgatcccca aacctttgct cgtcgccggc ggcaaaccat taatcgtctg
     3841 gcaggtagaa cgcctggtcc gggctggcat tacggaaata atcatcaatc atgcctggct
     3901 cggcatgcaa atcgaatgca ccttgcaaga cggccggcaa tacggcgccg ccatcactta
     3961 ctccgccgaa tcccccgcgc tggagactgc gggcggcatc gcaaaggcgc tggacttttt
     4021 tcacgggcgg ccttttctag tggtcaatag cgatatctgg tgcgactggg atccggtgca
     4081 tgccagacgg ctcgggcaaa tgcttctaaa cgaacaaaaa ctggcgtggc tattgctcgc
     4141 cgacaatccc ccgcaccatc ccgcgggcga ttttttactg aacgccgcgg gtctcgtcgt
     4201 ggaccgggca agccccgaag gcggtgcgcc gctgacattt accggtatcg gcatttacca
     4261 gcccgcattg ttcgaaaacc tgaacaaaaa acagccggcg cctctggcgc cgctattgcg
     4321 ccaggcgatg cgacagaacg ttgtagccgg
//
LOCUS       DATCXU010000212         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_265798,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000212 DATCXU010000000
VERSION     DATCXU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265798"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VIR04_04765"
     CDS             <1..362
                     /locus_tag="VIR04_04765"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573686.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY9460091.1"
                     /translation="AGARLLEILSRHDDPSAVLHALPQGISTPELKLVMQEGEPHALI
                     KRLQDNGQFPRAQSINTLDGVRVDYCDGFGLARASNTTPVVVLRFEGDTQDALECIQD
                     EFRQALMALAPTALLPF"
     gene            complement(376..1533)
                     /locus_tag="VIR04_04770"
     CDS             complement(376..1533)
                     /locus_tag="VIR04_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY9460092.1"
                     /translation="MADDFPQRHQTHPRRLLFVVNNPAFFLSHRLPLALAALQAGMDV
                     HVATMDGPSVPQIVAHGLSHHVIPLSRSGKNPVRELYSIYALWRLFRRLQPRLVHAVT
                     IKPVLYGGIAARAAGVPAFVAAISGLGFIFTRQRRHFDFLRWAATFLYGLALGHPNSR
                     VIFQNADDRDTLRSAGVVSSGQAVLIRGSGVDLAQFQAVPEPPGAPVAIMVARLLVDK
                     GVREFVAAARLAAGHPGGLRWVLAGSPDKGNPASVAYEEFAAWRREGVVECLGEQNDI
                     ASLYAKSHIAVLPSYREGLPKSLIEAAACGRPIVTTDVPGCRDAIEPGVTGLLVPARD
                     AKALCETVLLLAGDGALRREMGAAARRLAEREFDIRHVVQRHLDLYTSLMP"
     gene            complement(1654..2709)
                     /locus_tag="VIR04_04775"
                     /pseudo
     CDS             complement(1654..2709)
                     /locus_tag="VIR04_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2699..>3791)
                     /locus_tag="VIR04_04780"
     CDS             complement(2699..>3791)
                     /locus_tag="VIR04_04780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY9460093.1"
                     /translation="TLANAWARRGDSVTLTPTYTNRGSSFYFVSPAVDLVWLADRIGS
                     RRFPWLTGFHKWRALRRLIREKQPDLVISFLTNVNVMTLLSARGLRARVIVCERTDPA
                     VGATFGPILAGLRWLTYPWADLVTVQTQASVASFKKMVPGMRALAVIPNPLPPELLDF
                     PAALRGADTNGRRRLMAMGRLVPAKQFSCLISAFAELAASHPDWDLLIWGEGPLRDVL
                     SRQIQEAGLASRITLPGRTDAPWRELSQANAFVLTSAIEGFPNVLLEAMAIGLPCIAF
                     DCPSGPREITRGGQDALLIPLGDQKALVRSMNDLLGDADLRDRLALRALSVRDRYALP
                     AVLSIWDTVMRQLGVPARLQAAPTEKHDA"
BASE COUNT          873 a         1236 c         1034 g          648 t
ORIGIN      
        1 atgcgggcgc ccgcctgctt gaaatacttt cccgccacga cgatccgtca gcggtgcttc
       61 acgcgcttcc gcaaggcatt tcgacacccg aactaaaact ggtcatgcaa gaaggggagc
      121 cacatgcatt gatcaagcgc ctgcaagaca atggccaatt cccacgagcc caaagcatca
      181 ataccctgga cggcgtgcgg gttgattatt gcgatggctt cggcctggcc agagcatcca
      241 acacaacgcc cgtggtcgtg ctgcgcttcg aaggcgatac acaagacgcg cttgaatgca
      301 ttcaggacga attccggcaa gcgctgatgg cgcttgcgcc caccgccctc ttgccgtttt
      361 aacaattggc ctatttcacg gcatcaggct tgtgtataaa tccaggtgtc tttgcaccac
      421 gtgacgaata tcgaattccc tttcggccag ccggcgcgcc gccgcgccca tttcgcgacg
      481 caaggcgccg tctccggcca aaagcaagac tgtctcgcac aaggcttttg catcccgcgc
      541 gggaaccaat agccctgtga cccccggttc gatggcatcg cggcaacccg gcacatcggt
      601 ggtgacaatg ggtcgcccac aggccgccgc ttcgatcagc gactttggca agccctcgcg
      661 atacgatggc aacaccgcaa tatgcgattt cgcgtacagc gaggcgatgt cattttgctc
      721 gcccaggcat tcgactacac cttcgcgccg ccaggcggcg aattcttcat aggcgacact
      781 ggcgggattg cctttatcgg ggctgccggc cagcacccag cgcagcccgc cgggatgtcc
      841 agccgccagc ctggccgccg ccacaaattc cctgacgccc ttatcgacca gcaggcgggc
      901 aaccattatc gcaaccggcg cgccgggcgg ctcggggaca gcctgaaatt gcgccagatc
      961 aacacccgat ccacgaataa gaacagcttg cccggagcta acgacgcctg cactgcgcaa
     1021 agtgtcgcga tcatcggcat tttgaaaaat cacgcggcta ttgggatggc ctagcgcaag
     1081 gccatacagg aaggtggcgg cccaccgtaa aaaatcaaaa tgccgccttt gccgcgtaaa
     1141 aataaaaccc aggcccgaga tagccgccac gaacgcgggc acgcccgccg cccgggcggc
     1201 gatgccgcca tacagcacgg gcttgatggt gacggcatgc accagacgcg gctgcagcct
     1261 tcgaaacaga cgccataacg cgtagatgct atagagttcc cgcaccggat ttttaccgct
     1321 gcgcgacaag ggaatgacgt ggtgcgacag gccgtgcgcc acgatttgag gaactgacgg
     1381 cccgtccatg gtagcgacat gaacgtccat tcctgcctgc aatgccgcca aggcaagcgg
     1441 caagcgatgc gacagaaaaa aagccggatt attcaccaca aacaacaagc gtcgcggatg
     1501 tgtctgatgc cgctggggaa agtcatcagc caggcgtcgc catacattcc ggtaggctga
     1561 caccatcgtt tcgagactga atagattccc gacgcgctcg cgccccagtc tgattcgttc
     1621 cgccatttca gacgtgccca gcaaagacac agcctgattg atggcgtgcg ccaaagcgtc
     1681 cgcgtttcgc ggcggcgcaa tccagccgta atcgccgaca attctcgccg catcgcccac
     1741 attggtcacg acacacgcga cgcccgttgc catcgcttcg gccaccacat tcggaaatcc
     1801 ttcggcgcaa ctggataaaa cgtgcacgtc caaagcattc atgacgtcgg gaatgtcatc
     1861 tcgctctccg agcaggatta ctttaccgcg caaaccgagg cgatcaatca aagacatcaa
     1921 cgggacattg gattcatcca tgccgttgcc ggccaggaca cagcgcaatg acgtgtttgc
     1981 tgccgcgcaa agcgctgtcg ccgcaagcag attcgcgtga tctttcaggg gattccagcg
     2041 cgccaccgcg ccgataagca ccgtctcgtg cgccagaccc cattgcgccc tgacccgagc
     2101 ccgcgccgcc aggttgggct gccagcgcga taaatcatag ccatttggaa taaccagcat
     2161 ccgatccgcc cgatagcccc attgccgatg gcgaaccgcc gcattttcgg cgcacaccac
     2221 aatcacggcc ggcacgacgc cggaaagccg ggcgcacacc cacccgacga tacgggcttt
     2281 gagactgctt tcccgcaggt gtgcgcccga attccgaatt ccccatgaca ctgacctgac
     2341 gcccgccaaa cgcgccacca cgccgccgaa aagatccgcg tgatacatcc atgtttgaac
     2401 aacatcgggc ttcatttcac gcaacaaacg gtgcaaacgc gccaaaccgc ttaatgacag
     2461 cctgcccgac gacatgccca gagcgaccac cttgattccg gccgattgca aacgcggccc
     2521 aaagacaccc ttgtcagtca aggaaatcac ggtatgccgg cagctctgcg ccggcgcggt
     2581 aaccaaacgg tgcaaaaccg tctcggcgcc tccctggccc aggcccgaaa taatatgcac
     2641 aatatgcaaa caaggctctg gcatacaccg ttccggcggc aagggcggcg ttggcgaatc
     2701 aggcatcatg tttctccgtc ggtgcggcct gcagccgcgc cggcacgcca agttgccgca
     2761 ttactgtgtc ccagatcgaa agaaccgccg gtaaagcgta tcgatctcga accgaaagcg
     2821 cgcgaagcgc cagccgatcg cgcaaatcgg catcgcccag cagatcattc atgcttcgca
     2881 ccagcgcctt ttgatctcca agcggaatca aaagcgcgtc ctgcccgccc cgcgttatct
     2941 cacgcggacc gctggggcaa tcaaacgcga tacaaggcaa acctatagcc atggcctcga
     3001 gcaatacatt ggggaaaccc tcgatcgccg atgtcaagac aaaggcattg gcctgcgaca
     3061 gttcgcgcca tggcgcgtcg gtgcggcccg gcagggttat gcgcgatgcc aacccggcct
     3121 cttgaatctg tcggctcaaa acatcgcgta aaggcccctc gccccaaatg agcaaatccc
     3181 agtcgggatg actggcggca agctccgcaa acgcgctaat caggcaggaa aattgtttgg
     3241 ctggaacaag ccgccccatg gccatgaggc ggcggcggcc gttcgtatcg gccccacgca
     3301 aagcggcagg aaaatcaaga agctccggcg gcaatgggtt cggtattacc gccagcgccc
     3361 gcatgccggg caccattttt ttaaacgatg caacactggc ctgcgtctgc accgtgacga
     3421 ggtccgccca tggataagtc aaccagcgca atcctgcaag aatcggacca aaggttgcgc
     3481 ccacggccgg atcggtgcgc tcgcagacga tcactcgcgc gcgcagtccg cgggcgctca
     3541 atagagtcat gacgttaacg ttggttaaaa acgagatgac caaatcgggc tgcttttcgc
     3601 ggatgaggcg tcgcagcgcc ctccatttgt gaaagcctgt cagccacgga aagcgccttg
     3661 atccaatccg atcggccagc cagaccaaat caacagcggg cgacacaaaa taaaaggacg
     3721 acccgcgatt cgtataggtg ggcgtcagcg ttactgaatc gccgcgccgc gcccaggcgt
     3781 tggccagcgt g
//
LOCUS       DATCXU010000213         9934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_473573,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000213 DATCXU010000000
VERSION     DATCXU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473573"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VIR04_04785"
     CDS             complement(<1..919)
                     /locus_tag="VIR04_04785"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY9460094.1"
                     /translation="MFVVKTLTALVKSHALAGKRVFIRSDLNVPMNDRLEITEDTRIR
                     ASVPAIRMALDAGAAVMVTSHLGRPTEGKASQADSLAPIASRLQVLLGQPVPLIRDWV
                     DGVKVDAGQVVLLENCRFNVGEKKNDDALARKMAALCDVYVNDAFGAAHRIQASTYGM
                     ALYAPVACAGPLLEAELVALSRVLHEPARPLVAIVGGAKVSTKVTILRALAEKVDQLV
                     VGGGIANTFMLAAGLGIGRSLAEPEALEQAKAIMAIMKARGAEVPLPVDVVCGKTFDA
                     DATGATKAADQVADDDLILDIGPNTAQRLG"
     gene            complement(998..2011)
                     /gene="gap"
                     /locus_tag="VIR04_04790"
     CDS             complement(998..2011)
                     /gene="gap"
                     /locus_tag="VIR04_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY9460095.1"
                     /translation="MAIRVAINGYGRIGRNILRAHYEDGKKHDIQIVAINDLGDPKTN
                     AHLTRHDSAHGKFPGTVDVDGDYMVINGDKIRVLASRDPATLPWKELDIDVVFECTGL
                     FTTKEKAMAHIRGGAKKVIISAPGGKDVDATIVYGVNHKVLKASDTVISNASCTTNCL
                     APLVQPLHQKLGLVAGLMTTIHSYTNDQVLSDVYHKDLRRARSATTSMIPTKTGAAVA
                     VGLVMPDLNGKLDGYSIRVPTINVSIVDLSFTASRETSVEEVNGILKAASESSALKGI
                     LEYSTEPLVSVDYNHNPASSTVDSALTKVVGSLVKVCSWYDNEWGFSNRMLDTTVALM
                     SAK"
     gene            complement(2026..4041)
                     /gene="tkt"
                     /locus_tag="VIR04_04795"
     CDS             complement(2026..4041)
                     /gene="tkt"
                     /locus_tag="VIR04_04795"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY9460096.1"
                     /translation="MANAIRALAMDAVQQANSGHPGAPMGMAEIAQTLWTRHLRHNPA
                     DPAWADRDRFVLSNGHGSMLLYALLHLTGYDLPIGELKNFRQLHSRTPGHPEVGLTPG
                     VETTTGPLGQGLANAVGMALAEALLALEFNREGHTVVDHYTYAFAGDGCMMEGISHEA
                     CSLAGTLKLSKLILFYDDNGISIDGPVKNWFGDNTPGRFESYGWHVLRDIDGHDIAAV
                     DAAIVKAKQFAALRQGPTLICCKTVIGKGAPHVAGTERVHGSALGADEIAATRVALNW
                     PYAPFEIPAEIYRQWDAREKGQRLQADWQLRFDAYAKAFPVLAAELLRRLAGDLPDNF
                     AEQAAAYIAATALKAETVASRKASQFAIAAFVHVLPELLGGSADLTASNLTDWKGAEP
                     VRTNDQGLQPGHYINYGVREFGMTAIMSGIALHGGYFPFGGTFLTFSDYARNAVRMAS
                     FMRQRVIYVFTHDSIGLGEDGPTHQSIEHAASLRLIPNLHVWRPCDTTETAVAWAQAL
                     ERPASLGMNKKEGGPSALLLSRQNLPFVERDAATMKAISRGGYVLRDAPNARAIIIAT
                     GSEVALALGAQEQLAGKGIAVRVVSMPCTNVFDEQDESWRAAVLPEGLPRVAVEAGVT
                     SGWRKYVGLGGAVVGIDTYGESAPAGALFSYFGLTAHHVAAAVERIL"
     gene            4263..4997
                     /locus_tag="VIR04_04800"
     CDS             4263..4997
                     /locus_tag="VIR04_04800"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY9460097.1"
                     /translation="MATPRFYSPVPLAALQTIELAPELAHHAIRVLRLKADTEIILFD
                     GRGGEYPARLLIDGKSGYAQTGAHRPVEVELEGNITLVQGIPAGDKMDWVVEKAVELG
                     VKRLIPITAERGVSQLNAERGMKRRLRWERIAQTASEQCGRNRLMQIDAPQSLQNCMD
                     DITRDAQTLLFCHPEANKTLAQALQPGMASLTLLVGPEGGWSAAEQALALRHGLQAVK
                     FGNRVLRTETAGLALIAACSALQHWH"
     gene            complement(5073..6446)
                     /locus_tag="VIR04_04805"
     CDS             complement(5073..6446)
                     /locus_tag="VIR04_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460098.1"
                     /translation="MALNTFTRGQTDAPRVTHILAQFVANHPSRGWSDAVEHEAHRTF
                     INWLGCGIGAANHTTVTSVLAAVRELEPAAQATVLGRTERVDMASAALVNGVSSHTFD
                     YDDTHLKTIIHPAGPVASAVLALAEKTGSSGREAIDALVLGIDVSCRIGNMIYPDHYD
                     HGWHITGSTGMFGAAAACARLLKLDTEQTTMALGIAASQPVGFREQFGTMTKPMHPGA
                     AARAGMMSALMAKQGFTASPRAIEAPRGFAQVISNKFDWNEITDELGQRFEISFNTYK
                     PFACGIVIHPSIDGCIRMRTEHGLKPEDVARVELKVHGLVLELTGKKTPKTGLEGKFS
                     VYHGCAVGLAFGRAGEAEYADEIVTRRDVVALRDKVVATVDNSMAEAAADVTVLCTDG
                     RRFHVYVDHAVGSLENPMTDLLLEAKFDALVNPVLGPAKGQMLKSLSWKLADQPTLLE
                     LTSHAHP"
     gene            6755..7801
                     /locus_tag="VIR04_04810"
     CDS             6755..7801
                     /locus_tag="VIR04_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460099.1"
                     /translation="MSFSKSLIATMLASVSVWTGASCAQAPAPYDVTPQLVQAAQKEA
                     KVVFYTSIDLKVAEKLAQGFQAKYPGIKVQVERSGAERNFQRINQEYGSKIYTADVVE
                     SSDAVHFVYFKNKGWLEPAVPSDVAQWPKDEKDPDGQYAAVRATLSVPAYNTTLVKKE
                     DAPKSYKDLLDPKWKGKIVKSHPGYSGSTMTDTYVLSKTLGWDYFKKLGQQNIMQVQS
                     STVPPAKVAQGERAIEADGNEYVAFALKESGAPLEIIYPVEGSPLVVGNAGLLKKAPH
                     PNAARLFYHYIFTLEAQQSNSDVGGLRSFHAGVKEKAGRKPLSEIKLLHSDPAALGKE
                     VESIKKQYAMYFGT"
     gene            7854..8900
                     /locus_tag="VIR04_04815"
     CDS             7854..8900
                     /locus_tag="VIR04_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460100.1"
                     /translation="MLIRKSFIAAMLAGASMWGGASFAQAPAPYGVTPQLVQAAQKEG
                     KVVFYTSIDLKVAEKLAQGFQAKYPGIKVQVERSGAERNFQRINQEYASKIYNADVVE
                     SSDAVHFIYFKGKGWLQPAVPTDVALWPKDEKDPDGQYAVVRATLSVMAYNTTLVKKE
                     DAPKSYKDLLKPKWKGKIVKAHPAYSGTIMTDTYVLSKVLGWDYFKKLGQQDVLQVQS
                     ATVPGQKTAQGERAIMADGVEYVVFGLKESGAPIEPIYPTEGSPLVVGNAALLKKAPH
                     PNAGRLFYHYIFTLEAQQSDSDVGGLRSFHPGIKEKAGRKPLSEIKLLHSDPKALENE
                     VESVKKHYAMYFGT"
     gene            9362..>9934
                     /locus_tag="VIR04_04820"
     CDS             9362..>9934
                     /locus_tag="VIR04_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY9460101.1"
                     /translation="MAIINDGQLRKPRLHIDLFQPAFFLLAAVLVLLILLPLSWLVYY
                     SLVDKEGAFTFGNFINLITDRTLMEPFLRSLYMSAGVGALCCIVATPLAWLSARTDLP
                     GRRLLRALVMASFVTPPFLGAIAWEILAAPNSGLINELFRYITEQPRSEHLINIYTMQ
                     GLIFVISCYTFPYVFTLVANGLDRVPSDLED"
BASE COUNT         2325 a         2910 c         2618 g         2081 t
ORIGIN      
        1 ttccgagccg ctgtgcggtg ttcggcccga tgtccagtat caaatcgtca tcagccacct
       61 ggtccgccgc tttcgtggca cccgttgcgt cggcatcaaa agttttcccg caaacgacgt
      121 cgaccggaag cggcacctcg gcgccgcgcg ctttcataat cgccataatc gctttagctt
      181 gttcaagcgc ttcaggttcg gccaacgacc gtccaatgcc aaggccggcg gccagcataa
      241 acgtgttggc gatgcccccg cctaccacca attgatcgac tttttcggcc agcgcccgca
      301 agatggtcac cttggtggaa actttggccc caccgacaat ggccacgagc ggccgcgcag
      361 gttcgtgcag cacacggctc aaagccacaa gttcagcttc aagcaaaggc cccgcgcaag
      421 caaccggggc ataaagagcc atgccatacg tggaagcctg gatccggtga gccgctccga
      481 aagcgtcgtt tacataaaca tcgcacaacg cagccatttt gcgggccaac gcatcgtcat
      541 ttttcttctc gcccacattg aagcggcagt tttccagaag caccacctga ccagcgtcca
      601 ccttgacgcc atcgacccaa tcccgaatca gcggcaccgg ctgccccagc aaaacctgaa
      661 ggcggctggc gatcggcgca agcgaatcag cctgacttgc cttaccttcg gtcgggcggc
      721 ccaaatgaga cgtaaccatg accgccgccc ccgcatccaa agccatgcga atcgccggca
      781 cagaggcgcg aatgcgtgtg tcctcggtga tttcgagccg gtcattcatg ggcacgttca
      841 aatcggaacg gataaatacg cgcttgccgg ccagcgcgtg cgacttgacc aaagctgtaa
      901 gagttttaac aacaaacata gtttatgcac ccagacaaat ttacagacac gacaagggcg
      961 ggtttccccg ccccccgttc aaacgagcaa ccgcttacta tttagccgac atcaatgcga
     1021 ctgttgtgtc gagcatgcga ttggagaaac cccactcgtt gtcgtaccag gaacatactt
     1081 tcaccaacga gccaaccacc ttggtcaacg cggagtccac cgtactggac gccggattat
     1141 ggttgtagtc gaccgacacc aaaggctccg tgctgtattc aaggatgcct ttgagcgcac
     1201 tgctctcgga cgcggctttc aaaatcccat tgacttcttc caccgacgtc tcgcgcgacg
     1261 ccgtgaacga taaatcgacg atcgatacat taatagtcgg cacccggatc gagtagccat
     1321 cgagcttgcc attaaggtcg ggcattacca gacccacagc aaccgcagcg ccggtttttg
     1381 tgggaatcat gctggtggtg gccgaacggg cgcgacgcaa atccttgtga tatacatccg
     1441 acaagacctg atcgttggta taggaatgaa tagtcgtcat caggccggcc accagaccca
     1501 atttctggtg caagggctga accagcggcg ccaggcaatt ggttgtgcaa gacgcattgg
     1561 aaataaccgt atcgctcgct ttcagaactt tgtgattgac cccataaaca atcgtcgcat
     1621 cgacatcttt gccgccggga gcggaaataa tcactttttt tgcgccgccc ctgatgtgag
     1681 ccattgcctt ttctttggtg gtaaacaacc ccgtgcattc gaacacgacg tcgatatcaa
     1741 gttccttcca tggcagcgta gcgggatcac ggctggccag cacccgaatc ttgtcgccgt
     1801 tgatcaccat atagtcgcca tcgacatcga ccgtacctgg aaacttcccg tgcgcagaat
     1861 catgacgcgt taaatgcgca ttcgtcttgg gatcgcccaa gtcgttaatg gccacaattt
     1921 gaatgtcatg ttttttgccg tcctcgtagt gggcacgcaa aatgttgcgt ccaatacgcc
     1981 catatccgtt aattgccacg cgaatcgcca ttcttactct cctgtttata aaattcgttc
     2041 aacggctgcc gcaacgtggt gggcagtcag gccaaagtag ctaaataacg cgcctgccgg
     2101 ggcggattcg ccataagtat cgatccccac cacggcgccg ccgagtccga catatttgcg
     2161 ccaacctgat gtcacgcccg cttcaaccgc cacccggggc aatccttcag gcaataccgc
     2221 cgcgcgccag ctttcgtctt gctcatcgaa cacatttgtg caaggcatgg acacgacccg
     2281 aacggcaatc cccttgcccg ccagttgctc ctgcgccccg agggcaagcg ccacttcgga
     2341 gccggtcgca atgataattg cccgggcatt gggagcgtcg cgcaagacat agccgccgcg
     2401 ggaaatcgcc ttcatcgttg ctgcgtcgcg ttccacaaaa ggcaaatttt gccgcgacaa
     2461 caacaacgcg ctcgggccgc cctctttctt gttcatgccc aggctcgccg ggcgttcaag
     2521 cgcctgggcc caggcgactg ccgtttcggt ggtgtcgcaa ggacgccata cgtgcaaatt
     2581 gggaatcagt cgcaagctgg cggcatgctc gatcgattga tgtgtcgggc cgtcttcgcc
     2641 caagccaatc gagtcgtgcg taaacacata aattacacgc tgcctcatga acgacgccat
     2701 gcgcaccgcg ttgcgggcgt aatccgaaaa agtcaggaac gttccgccaa aaggaaagta
     2761 gccgccatgc agcgctatgc cgctcatgat ggccgtcatc ccgaactcgc gcacgccgta
     2821 gttaatatag tgtccaggtt gcaacccctg gtcgttggtc cgcacgggct ccgcaccctt
     2881 ccaatcggtc aggttcgacg cggtcagatc ggccgaaccg cccagaagct cgggcaagac
     2941 gtgaacgaaa gcggcaatgg caaactgcga tgctttgcgg cttgcaaccg tttcggcctt
     3001 gagggctgtc gcggcaatat acgccgccgc ctgttcagcg aaattgtctg gcagatcgcc
     3061 ggccaagcgg cgcagcagtt ccgcggccaa aaccggaaat gcctttgcat aagcatcaaa
     3121 gcgaagctgc cagtcggcct gcagacgctg tcctttctcc ctggcgtccc attgccggta
     3181 aatttcagcc ggaatctcaa atggcgcata aggccaattc agcgcaacgc gcgtggccgc
     3241 gatttcatcg gcccccagcg ccgagccgtg caccctttcg gtgcctgcaa cgtgtggagc
     3301 gcctttccca ataacagtct tgcagcagat caatgtcgga ccttgccgca aagccgcgaa
     3361 ctgttttgct ttgacaatag cggcatccac tgcggcaata tcgtgaccgt caatatcgcg
     3421 caacacgtgc cagccataac tttcaaagcg gccgggagta ttgtcgccga accagttctt
     3481 taccggcccg tctatggaga tgccattgtc gtcatagaac agaatgagct tggatagttt
     3541 gagtgtgccg gcaagcgagc acgcctcgtg cgatatccct tccatcatgc agccgtcgcc
     3601 ggcaaacgcg taggtatagt gatcgacaac ggtgtgacct tcgcgattga attcaagcgc
     3661 cagcaaagcc tcggccaggg ccatacccac cgcattggcc aacccttgtc ccaatggtcc
     3721 ggttgtggtt tcaacccctg gggtcaaacc cacttcggga tgcccaggcg tgcgcgaatg
     3781 caactgcctg aaatttttca gctcgccaat gggcaggtca tagcccgtga ggtgcagcaa
     3841 ggcgtaaagc agcatcgacc catgcccatt ggacagcaca aaccgatcac ggtcggccca
     3901 ggcagggtcg gcaggattat ggcgcagatg gcgcgtccat agtgtctggg caatttccgc
     3961 cattcccatg ggggcgccgg gatggcccga attggcttgt tgaaccgcgt ccatggcaag
     4021 agcgcggata gcgtttgcca tggcagaata agggacgggc gataagctca ttaaaaactc
     4081 acaaaaagcg acatggcaaa gagctgccgc gggcaatcgg ggacacaaaa acaaaacgtt
     4141 caattttacc atcgcaggcg cggccaccgc acaaggcgcc agtttactga accaagcgcc
     4201 ggtttacact atggcgattg gccatcccgc acaattacgg aacccgaaga caacgatttt
     4261 ttatggctac tccccgcttt tacagtcccg ttccgctggc ggcgcttcaa acaattgaac
     4321 tcgctcccga gctcgcgcac cacgccatac gtgtgctgcg acttaaggcc gacacagaaa
     4381 tcattctgtt cgacggacgc ggaggggaat accccgcgcg attgctgatc gacgggaaaa
     4441 gcggctacgc gcagaccggt gctcatcgcc ctgtcgaggt ggagctggaa ggaaacatca
     4501 ccctggtgca aggcatcccc gcgggcgaca aaatggactg ggttgtcgaa aaggcagtcg
     4561 aactgggagt caagcgattg attccaatta ccgcggaacg cggcgtatca caactgaacg
     4621 ccgagcgcgg aatgaaacgc cgcttgcgtt gggaacgcat cgctcagacc gccagcgaac
     4681 agtgcggacg caatcggctc atgcaaatcg acgcgccgca gtccttgcaa aattgcatgg
     4741 acgacatcac tcgggatgca caaacactat tattctgcca ccccgaggca aataaaaccc
     4801 tggcgcaggc attgcagccg ggtatggcca gcctgacgct tttagttggc cccgaaggcg
     4861 gctggtccgc cgcggagcaa gcgttggcgt tgcgccacgg gttgcaggcc gtcaaattcg
     4921 gcaatcgcgt cttgcgcact gaaacggctg gcttggcgtt aatagccgct tgcagcgcgc
     4981 tgcagcactg gcattagcgt tcctcttcct aatgatgtcc catctccccc gggcaactac
     5041 agagccaggg accggcaggc gatacgcttc attcagggat gggcatggct tgtcaactca
     5101 agaagagtgg gttgatcagc caatttccag cttaaactct tcagcatctg gccctttgcc
     5161 gggcccagaa ccggattcac cagggcgtcg aactttgcct caagcagcaa atccgtcatc
     5221 gggttttcca ggcttcccac ggcatggtcg acatacacat gaaaccggcg tccgtcggtg
     5281 cagagtaccg ttacatcggc ggccgcttca gccatggaat tgtccaccgt ggcaaccact
     5341 ttgtcgcgca atgcaaccac atcgcggcgc gtgacaattt catcggcata ctcggcctcg
     5401 cccgctctgc caaacgcaag gccaacggca catccgtgat aaacgctgaa cttcccttcg
     5461 aggccggttt tgggcgtctt ctttccggtt aattccagga caagaccatg caccttcagc
     5521 tcaacgcgcg caacgtcttc gggtttcagc ccgtgttccg tacgcatgcg gatgcaaccg
     5581 tcaatgctgg gatgaatcac gatcccgcat gcaaacggct tatacgtatt gaaggaaatt
     5641 tcaaaccgtt gtccaagttc gtcggtgatc tcattccaat cgaatttatt cgaaataact
     5701 tgagcaaaac cgcgcggcgc ttcaattgcg cgcggcgatg cggtaaaccc ttgtttggcc
     5761 atcagggccg acatcattcc cgcgcgcgcc gcagcgcccg gatgcatggg tttggtcatc
     5821 gtgccaaatt gctcccgaaa ccccactggt tgcgaagccg cgatgcccag cgccatcgtc
     5881 gtttgctcgg tgtcgagctt taacaagcgc gcacaagccg ccgccgctcc gaacatgccg
     5941 gtcgaaccgg taatatgcca gccgtggtcg tagtgatccg gatagatcat gttgccgatc
     6001 cggcacgaaa catcaatgcc cagcaccaag gcgtcgatcg cctcgcgacc cgaagaaccc
     6061 gtcttttccg cgagcgccaa aacagccgag gcaaccggcc cggcgggatg gataatcgtt
     6121 ttcaaatgcg tatcgtcgta gtcgaaagta tgcgaagaaa cgccattcac caacgccgcg
     6181 ctggccatat ccacacgttc cgttcgaccc aggaccgtcg cttgcgcggc aggttcaagc
     6241 tcccttacgg ccgccaatac cgaagtaacc gtagtatggt tcgccgcccc aatcccacac
     6301 cccaaccagt tgatgaaggt gcggtgcgct tcatgttcaa cagcgtcgct ccaccccctg
     6361 gacggatgat tagcgacgaa ttgcgccagt atatgggtaa cccggggagc atcagtttgc
     6421 cctctggtaa aggtatttag tgccatgttt tcctgcgttg aataagagat gggctcagcg
     6481 taacagcgcc aataaccagc cacttgcggg cgtgttgctc tgcatactgt taatagtcta
     6541 caatattttc agagtctaaa gaagtttctc tctatacggc ccggacgggt cggtctatga
     6601 tttgaaattg cgtcgcaaag aaagccatac aataaattcg cgggttggat tcgatttcca
     6661 aagatcgata agtgcataaa aagtagatgg cttccttggg tgtgtttcaa acgttggcta
     6721 atatagtttc caatcactag tacaggagac agctatgtcg tttagtaagt cactcatcgc
     6781 cacgatgctt gcaagcgtgt cggtgtggac gggtgcgtcc tgcgcgcagg cgccggcgcc
     6841 ctatgatgtc acgccgcaat tggttcaggc cgcccaaaaa gaggccaagg tggtgttcta
     6901 cacctcgatt gatcttaaag tggccgaaaa gctggcgcaa ggctttcagg cgaaataccc
     6961 cggcatcaaa gtgcaggtgg agcgctccgg agccgagcgc aatttccagc gcatcaacca
     7021 ggaatatggc agcaaaatct acaccgctga cgtggtggaa tcctcggatg cggtccactt
     7081 cgtttacttc aagaacaagg gctggcttga acccgccgtt ccttccgatg tggcccagtg
     7141 gcccaaggac gagaaagatc ccgacggcca atatgcggct gttcgcgcaa ccctgtcggt
     7201 tcctgcctac aacactacgc tggtcaagaa ggaagacgcc cctaaaagct ataaggattt
     7261 gctcgatcca aaatggaaag gcaagattgt gaagtcgcat ccgggctata gcggcagcac
     7321 catgaccgac acttacgtgc tcagcaaaac cctgggctgg gactacttta aaaagctggg
     7381 ccagcaaaat attatgcagg tccaatcatc caccgtgcct cctgcgaagg ttgcccaagg
     7441 tgagcgcgcc attgaagcgg acggcaatga atatgtggct ttcgccctga aggaatcggg
     7501 cgctcctctg gagataattt accctgtcga aggatctccg cttgtggtgg gcaacgccgg
     7561 tttgctcaaa aaggctccgc accccaacgc cgcgcgtctg ttctatcact acatattcac
     7621 gctggaagca caacagagca acagcgacgt cggcggactg cgttcattcc acgccggggt
     7681 aaaagagaaa gcgggccgca aaccgctgtc ggaaatcaag ctgttgcact cggaccccgc
     7741 agcacttggc aaagaagttg aatccatcaa gaaacagtat gcgatgtatt ttgggaccta
     7801 agctgcggtg acaaagcggc gacgatcata aaaacactac acaggagaca aatatgttaa
     7861 tacgtaaatc gttcatcgcc gcgatgctcg ccggcgcatc gatgtgggga ggcgcatctt
     7921 tcgcgcaagc gccggcgcct tatggcgtca cgccccaatt agttcaggcg gcccagaaag
     7981 agggcaaggt ggttttttat acctcaatcg atctcaaggt cgccgaaaag ctggcgcaag
     8041 gctttcaggc gaaataccct ggtattaaag tgcaggtaga gcgctcgggc gccgagcgca
     8101 atttccagcg catcaatcag gaatacgcca gcaaaattta taacgccgat gtggtcgaat
     8161 cctcggatgc ggttcacttc atctacttca agggcaaggg ctggctccag cccgctgttc
     8221 ccactgatgt cgcgctgtgg cctaaagacg aaaaggatcc cgatggccaa tatgcggtcg
     8281 ttcgcgcgac cttgtcggtc atggcctaca acaccacact ggtcaaaaaa gaagacgcgc
     8341 ctaaaagcta caaggatctg ctcaagccga aatggaaagg caagattgtg aaggcgcatc
     8401 cggcctacag cgggaccatc atgaccgata cctatgtcct gagtaaagtt ctgggctggg
     8461 attacttcaa gaaactcggc cagcaagacg tgctgcaagt tcaatccgcc acagtgccag
     8521 gacaaaaaac agctcagggc gagcgggcca ttatggccga tggcgttgaa tacgttgttt
     8581 tcgggctgaa agaatctggc gctccgatcg aaccaattta tccgaccgaa ggatcgccgc
     8641 ttgtagtggg caacgccgca ctgctcaaga aggcgccgca tcccaacgca gggcgcctct
     8701 tttatcacta tatattcacg ctggaagcgc agcaaagcga cagcgacgtt ggcgggctcc
     8761 gctctttcca tcccggcata aaagagaagg cggggcgcaa accgctatct gaaatcaaat
     8821 tgctgcattc ggatccgaaa gctcttgaaa acgaagtcga gtccgtcaaa aaacactatg
     8881 caatgtattt tgggacctga gcaggcaaca aaccgccaac atgtacacgc tcgcgctcag
     8941 ttctaagctg ggcgcgaacg catttagcgt ccccgccttg ctttgtagac tgtaaatcgt
     9001 ctacaataag catggatatc gggtgctcgt cttgtacgga acaacctcgc ttattccaat
     9061 gttcgccacg cttctgggac atttcaagag ccgtcttcaa acaacagttc cgtattgaaa
     9121 agtccctaca atacatataa attcggatct gaccgcgatg accctgtctt gcaaaaaaga
     9181 taagagcaaa tttctatttt gattagcctt ccaaacgcga gctccatcga atttcgataa
     9241 ctaataacag gagacaacca tgttgttgag caaatcagcc gccattgcaa cgctgcttgc
     9301 catggcgacg cggttgaatc aatcaaaaaa cattatgcaa actactttgg gacctgagta
     9361 aatggcgata atcaacgacg gccagcttcg gaagccgcgc ctgcatatcg acttgttcca
     9421 gcctgccttt ttcctgctgg cggccgttct tgttctgctt atcctgctgc cgttatcgtg
     9481 gctggtgtat tacagtctgg tcgacaaaga aggcgcattc acgttcggga atttcatcaa
     9541 tctgattacg gatcggacgc tgatggagcc gttcctgcgc tctttgtata tgtcggccgg
     9601 tgtgggcgcc ttgtgctgca tcgtcgccac tccgctggcc tggctaagcg cgcgaaccga
     9661 tctcccggga cggcgtctgc tgcgtgccct ggtgatggcc tcgtttgtca cacccccctt
     9721 tctgggcgcc attgcatggg aaattctcgc ggcgcccaac agcggcttga tcaacgaact
     9781 gttccgctac attaccgagc aaccgcgaag cgaacacctc atcaatatct acaccatgca
     9841 gggtctgatt tttgtcatct cctgctatac cttcccgtac gtgttcacgc tggtggccaa
     9901 cgggctggat cgggtgccat cggatcttga agac
//
LOCUS       DATCXU010000214         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_550819,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000214 DATCXU010000000
VERSION     DATCXU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550819"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VIR04_04825"
     CDS             <1..163
                     /locus_tag="VIR04_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEY9460102.1"
                     /translation="PFASGGVYVNFLTADEGDRVRSAYGQNYDRLARIKREYDPDNLF
                     HTNQNIGPA"
     gene            complement(175..1935)
                     /locus_tag="VIR04_04830"
     CDS             complement(175..1935)
                     /locus_tag="VIR04_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY9460103.1"
                     /translation="MNFKLIQNQRGAVTLIFLASSTLVLGASFGAIDLVRYNVVQSRL
                     QYALDGAALSAGRNLANLTPTPGTTAEGQWQADAFQYFRSNLPEGYLGSSVQPKDLTI
                     TYGEQLAGAGNYVTQQLINMDAKGVLPLISTGYFKISSFDIQASNQAVRSTRSDLELV
                     MALDNTGSMASDGKIDTLKAAAKQLSETVLGASAASGKPGRVFIGLVPFADTVNVGNT
                     DATRQWLVYPAEQSYFINNFWGGCIAEPPTPSWGGANLPAAALTPTAGFQPLHLTLDT
                     QLNGSDLGLASNKSASIYERIAPVLTGFPQPRSFDANGAAYDRRVWATRSSNTSFDIH
                     SAANPSNCVASRLVRFLSDDLNTINTAIDGMQANGNTLIPTGLLWGWRMLNPAWRGTP
                     GWGNPDMPRDPDSKVLSKVIVLLTDGQNDPPSSIGSLNGTERLFAYQLAYDAQSCTDK
                     KTTACSPTLLPRTYTQGKFTVPNVSQAPMNSLKMRDPFDTSGKNAVNPSIGWGDSTNI
                     TTATVDTYMQDVCANVKNDGNGIKIYTVTLGPVGSSAETQMNNCSSGAGYFYNATNVS
                     DLPDVFKSIAGALTELRLTS"
     gene            complement(1958..2560)
                     /locus_tag="VIR04_04835"
     CDS             complement(1958..2560)
                     /locus_tag="VIR04_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY9460104.1"
                     /translation="MINKSRHYRIRERGVAALEAALLLPVAVFLLFACLELYQYYRAA
                     ALLDRVAFTVADGVAMQRQLYNHNQCGNSDDICVYGAMAQDLFQPLNYALNGGMIISS
                     FAATDPDQNGNVTWKAQPEWHTTFKGSGSTLPDPSSQLTDKTSFPQAHVGDTIIVVEV
                     FYDHQPFVMSSKLWAALAGTSHMYSRFFFRPRFDDLRVLL"
     gene            complement(2564..>2909)
                     /locus_tag="VIR04_04840"
     CDS             complement(2564..>2909)
                     /locus_tag="VIR04_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460105.1"
                     /translation="EAAASRVARIGQLGVPAGLSCDEAVRGIFEKRMSNWVYDPSQLR
                     IDVKLYNPGGNNNLPNLDDPSYVPVCDTGGRGDIVVYRLGFDRPGFSGILNWLGIKLL
                     RFQRTVIIQNEP"
BASE COUNT          591 a          773 c          889 g          656 t
ORIGIN      
        1 gccgtttgcc agcggcggag tttatgtaaa tttccttacc gccgacgaag gcgatcgcgt
       61 acgttccgca tacggtcaga attacgatcg cctggcccgg atcaagcgcg agtatgaccc
      121 ggacaacctt tttcacacga atcagaacat cggacccgcc tgaagtcttt tgccctatga
      181 agtcagcctc aattcggtca gcgcgccggc tatggatttg aacacatccg gcaggtcgct
      241 gacgtttgtg gcattgtaaa agtagccggc tccgctcgaa cagttgttca tctgcgtttc
      301 ggccgaactt cccacgggcc ccagagtaac ggtgtagatt ttgatgccgt tgccatcgtt
      361 tttcacattc gcgcatacgt cctgcatgta ggtgtccacc gtcgcggtcg taatatttgt
      421 cgagtcgccc catccgattg aggggtttac tgcgtttttg ccgctcgtgt cgaacgggtc
      481 ccgcattttc agagagttca tgggcgcctg cgacacgttg ggcacggtga atttgccttg
      541 tgtgtaagtt cggggaagca gtgtgggact gcaggcggtc gtttttttgt ccgtgcaact
      601 ctgggcatcg taagccagtt ggtaggcgaa tagccgctcg gtgccattga ggctgcctat
      661 gcttgaagga gggtcattct ggccgtcggt gagcagcaca attactttgc tgagcacctt
      721 cgaatcaggg tcgcgcggca tatcgggatt accccatccg ggggtgccgc gccacgccgg
      781 attgagcatg cgccagcccc aaagaagccc ggttggaatc agagtgttgc cattggcctg
      841 catgccatcg atggctgtat tgatggtatt gaggtcgtcg ctcaggaagc ggaccaggcg
      901 cgacgctacg cagttggagg ggttcgcggc ggaatggatg tcgaagctgg tgttggagct
      961 gcgcgtggcc cagacccggc gatcataggc ggctccattc gcatcgaacg aacggggttg
     1021 cgggaagcca gtcaaaacgg gggcgatgcg ttcgtagatg ctggcggatt tattgctggc
     1081 caggccaagg tccgacccgt ttagttgggt gtccagggtt agatgcaatg gctgaaatcc
     1141 ggcggtgggc gtcaaggctg ccgcgggcag atttgcgccg ccccacgaag gagtgggcgg
     1201 ctcggcgatg cagcctcccc agaagttatt gatgaaataa ctttgctcgg caggatacac
     1261 aagccactgg cgcgtggcgt ccgtgttgcc gacgttgacg gtgtcggcga atggaaccag
     1321 ccctatgaaa acgcgccctg gctttcccga agcggccgat gcccccagca cggtctcgct
     1381 taattgcttt gcggcggcct tgagcgtatc gatcttgccg tcagaggcca tggatccggt
     1441 gttatccagc gccatgacca gctccaggtc agaacgtgtg ctgcgaacgg cctggttgct
     1501 ggcctgaatg tcgaatgaag atattttgaa ataaccggtg cttatcaaag gaaggactcc
     1561 tttcgcatcc atgttgatta attgctgcgt cacataattg cccgccccgg caagctgttc
     1621 gccataggtg atcgtgaggt ctttgggttg tacgctgctg cccagatagc cctccggcag
     1681 gttgctgcga aaatattgga aggcgtcggc ttgccactgg ccctccgcgg tcgtgccggg
     1741 ggtgggtgtc aggttggcca ggttgcggcc ggccgaaagc gctgcgccgt ccagtgcgta
     1801 ttgcagcctg gattgcacta cgttgtagcg caccaggtct attgctccga aggatgcgcc
     1861 cagcaccagg gtcgaactgg caaggaatat cagtgtcact gcaccccgtt gattttgaat
     1921 tagcttgaag ttcatcgttc tgccccgttt agaattgtca aagcaataca cgcagatcgt
     1981 caaagcgtgg ccggaagaaa aagcggctgt acatgtgcga cgtgccagcc agcgccgccc
     2041 acagtttgga actcattaca aaaggctggt ggtcatagaa cacttcgacg acgatgatgg
     2101 tgtctccgac gtgggcctgt ggaaaagaag tcttgtcggt caattgagat gacggatcag
     2161 gcagggtcga gcccgatccc ttgaatgtcg tgtgccattc gggctgcgcc ttccaggtga
     2221 cgttgccatt ttggtccgga tcggtggcgg caaaggagct gatgatcatg cctccgttca
     2281 gggcataatt gagcggctgg aacagatctt gggccatcgc gccatagacg cagatatcgt
     2341 cggagttgcc gcattgatta tggttatata gctgacgctg catggccacg ccgtccgcaa
     2401 cggtaaaggc aaccctgtcc agcagggcgg cggcccggta atattggtag agctcaaggc
     2461 aggcaaacag caagaatacg gcaacgggca ggagtagcgc cgcttccagc gcggcaacgc
     2521 cccgttcccg gatacgataa tgcctggatt tattaatcat ggttcaaggc tcattctgga
     2581 tgattacggt gcgctggaag cgcagaagct ttattcccag ccagttcaaa atacccgaga
     2641 atcctggccg gtcgaatccc agtcgataga ccacaatatc gccacgtccg ccggtatcgc
     2701 atacggggac ataagaaggg tcgtccaggt tgggcagatt attattgccg ccggggttgt
     2761 aaagcttgac gtcaatgcgc aattgcgagg ggtcgtacac ccagttgctc atgcgttttt
     2821 caaaaatgcc tcgtacggcc tcgtcgcagg aaaggccggc aggcacaccc aactggccta
     2881 ttcgtgcaac ccgcgacgcg gccgcctcc
//
LOCUS       DATCXU010000215         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_611289,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000215 DATCXU010000000
VERSION     DATCXU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611289"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIR04_04845"
     CDS             complement(<1..392)
                     /locus_tag="VIR04_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY9460106.1"
                     /translation="MDLNLCMDTKARVSNTEWQTRIDLAACYRLMPLFGMSDLIYNHI
                     TARIPGEKEHLLINPYGLMYEEITASSLITIDLEGNILLNPNRDCEINQAGYVIHSAV
                     HAARKDVHCVIHTHSRAGMAVSALKCGLL"
     gene            complement(477..1955)
                     /locus_tag="VIR04_04850"
     CDS             complement(477..1955)
                     /locus_tag="VIR04_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY9460107.1"
                     /translation="MQRYQMYIDGKFVDSSSGRWFDSYNPYTGKVWSQVAEGNAADAD
                     RAVLAAHKAFSEGPWSRLTATGRGALLRKLGDLIARDARKLAEIEVRDNGKLIAEMAG
                     QLNYIPQWFYYYGGLADKVQGAAIPLDKKGYFNFTRYEALGVVTIITPWNSPLLLTSW
                     KLAPALAAGCTVVLKPSEFTSASALEFVKLVEEAGFPPGVVNVVTGFGKDVGASLVEH
                     PLVRKISFTGSDATGRAINQQAAKHFKHVNLELGGKSPNIVFADANLDDAVNGAISGI
                     FAATGQTCIAGSRLLLQESIHDAFVEKLAALAKTARMGDPQNIETQVGPVTTRPQYDK
                     VLGYINIAKQEGAELLMGGAPATRAECGSGWFVEPTIFAGVKNTMRIAQEEVFGPVLS
                     VIKFKDEDDAISIANDSRFGLGSGVWTSDIGRAFRMAERIQAGTVWVNTYRAVSFMSP
                     FGGYKDSGMGRENGLDAIRDYLQVKSVWINTGVATSNPFTMR"
     gene            2210..3094
                     /gene="rpoH"
                     /locus_tag="VIR04_04855"
     CDS             2210..3094
                     /gene="rpoH"
                     /locus_tag="VIR04_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY9460108.1"
                     /translation="MTNHLQPLSLSNSTLALAISNPGALGTIQAYISAVNRVPMLTQE
                     QEFALGKRLHDDADLDAARQLIVSHLRLVVSVARQYLGYGLAHADLIQEGNVGLMKAV
                     KRFDPERGVRLVSFAVYWIKAEIHEYIIRNWRLVKVATTKAQRKLFFNLRRLRPDGQT
                     LDSTQVNDIAQELNVRPQDVSEMEVRLSGRDMAMDGRDDGDESFSPITYLSDNGHDDP
                     SRVLERRAHDTLQGEGLENALSDLDPRSRRIVQARWLQDEGGATLHDLANEFGVSAER
                     IRQIEVAAFKKMRSSLAA"
     gene            3363..>3811
                     /locus_tag="VIR04_04860"
     CDS             3363..>3811
                     /locus_tag="VIR04_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY9460109.1"
                     /translation="MQVGHLVFGLAGLLALVSFMPPLAGRLHLPHSVLLAIVGFLLGL
                     LIHIRGWAPSVMSDFLDSLQRFEISSEAFLFIFLPVLLFEMSLAMNVRRLLDDIGSIL
                     MMAVVAVVVCTVAVGFSISAVSDYGLVVCLMLGAIVATTDPVAVVGV"
BASE COUNT          769 a         1039 c         1057 g          946 t
ORIGIN      
        1 agcagaccgc atttaagagc cgacacggcc atgccagcgc gcgaatgcgt gtggattacg
       61 cagtgcacat ctttgcgcgc cgcatgcacg gcgctatgta tcacatagcc cgcctgattt
      121 atttcgcaat cgcgattggg attaagcaaa atattgccct ccaggtcgat cgtgatcaga
      181 ctggatgcgg tgatttcctc gtacatcaag ccatagggat tgataagcaa atgttctttt
      241 tcccccggaa tgcgcgcggt gatatggttg taaatcagat cgctcatccc aaacagaggc
      301 atcagacgat agcaggccgc caggtcgatt cgggtttgcc attccgtgtt cgaaacacgt
      361 gccttggtgt ccatgcataa attcaagtcc atcgcgattc ctcctgaagg gtagtagttc
      421 aattctaggc ctgatcgtcg aagccattcg caacaatcac gtcgatcaag tttggcttaa
      481 cgcatggtga aaggattgga ggtggcgact ccggtattga tccagacgct tttcacttgc
      541 aggtaatcgc ggatggcgtc caagccgttt tcgcgaccca tccccgaatc cttgtagccg
      601 ccgaatggcg acatgaaact gacggcgcga taggtattga cccaaaccgt gccggcttga
      661 atgcgttcgg ccatgcggaa agcccgcccg atatcgctgg tccagacgcc cgatcccaaa
      721 ccgaagcggg aatcgttggc aatggatatg gcgtcgtctt cgtccttgaa tttgatcacc
      781 gatagaacag ggccaaagac ctcttcctgg gcgatgcgca tggtgttttt cacgccggcg
      841 aatatggtgg gttcgacgaa ccagccgctg ccgcattcag cccgggtggc cggcgcgccc
      901 cccatgagca gttcggcgcc ttcctgtttg gctatattga tgtagcccag caccttgtcg
      961 tattgcggac gcgtggtgac cggacctacc tgtgtttcta tgttttgggg gtcgcccatg
     1021 cgcgccgttt tggccagcgc cgccaatttt tcgacgaagg cgtcgtggat gctttcttgc
     1081 agcaacagcc gcgaacccgc gatgcaggtc tgccccgtcg cggcaaaaat gccggagatg
     1141 gctccgttca cggcgtcgtc cagattggcg tcggcgaaaa cgatgttggg cgatttgccg
     1201 cccagttcca ggttgacatg cttgaagtgt ttggccgcct gctggttaat ggcgcgcccg
     1261 gtggcgtcgg aaccggtaaa cgaaattttt ctgacgagcg gatgttccac gagagaagca
     1321 cccacgtctt tgccgaaacc ggtaacgaca ttgaccacgc ccggcgggaa gcctgcttct
     1381 tcaaccaatt tgacgaattc cagtgcagag gcggaagtga attcggacgg cttaagtacg
     1441 accgtgcagc cggccgccag agcgggcgcc agtttccagg acgtaagcaa aagcggcgaa
     1501 ttccatggcg tgataatggt cacgaccccc agcgcctcgt agcgggtgaa gttgaaatag
     1561 ccttttttgt ccagtggaat ggccgcgccc tgaactttgt cggccagccc gccgtagtag
     1621 tagaaccatt gcggaatgta attaagctgg cccgccattt cggcgatcag cttgccattg
     1681 tcgcgcactt cgatttcggc cagtttcctg gcatcgcgtg caatcagatc gcccagcttg
     1741 cgcaatagtg cgccgcgccc ggtggctgtc agcctggacc aggggccctc gctgaatgct
     1801 ttgtgcgccg ccagcacggc gcgatccgcg tccgcagcgt tcccttcagc gacctggctc
     1861 catactttgc cggtataggg gttgtagctg tcaaaccatc gtcccgatga tgagtcgaca
     1921 aatttaccgt caatatacat ttggtagcgt tgcattgtgt gtcccttcgt tgatttggcg
     1981 agtatgtcgc actaacatta ttatttggcg ccgtgttagc cacttcgtcg ccagtctggc
     2041 ttgattttag gtgtttcacc gaaacatcgc agtttaaaaa tggtgaaccc acgcttcgcc
     2101 cgcgcctaat gggaactttt gacccacgcg cgactctcac aattagcact ccttatcata
     2161 gagtgctaac atgtattcag tcgatctttt cgaaaggacc gcgtattcga tgaccaatca
     2221 cttacaacct ttgtcattgt ctaacagcac gctggctttg gccatttcca atcccggcgc
     2281 attgggcacg atccaggcct acattagtgc cgtcaatcgt gtacccatgc tcacacaaga
     2341 gcaagaattc gcactgggca agcgtttgca cgacgacgcc gatctggacg ccgcgcgaca
     2401 actgatcgta tctcatcttc ggcttgtggt atcggtggcc cgccaatatt tgggctatgg
     2461 gctggcgcat gccgacctta ttcaggaagg caatgttggc ttgatgaagg cggtcaaacg
     2521 cttcgatccg gaacgaggcg tgcgtctggt ttcctttgcc gtctactgga tcaaggcgga
     2581 aattcacgaa tatattattc gcaactggcg cctggttaaa gtggccacca ccaaggcgca
     2641 acgcaaattg ttttttaatt tgcgcagatt gcgtcccgat ggtcaaacac tcgactcaac
     2701 gcaagtaaac gatatcgcgc aggaactgaa tgtgcgtccg caggacgtca gcgaaatgga
     2761 agttcgtttg tccggccgcg acatggccat ggatggccgt gacgacggcg acgaatcatt
     2821 ttctcccatt acttatttat ccgataacgg tcacgacgat ccatcgcgcg tgcttgaacg
     2881 ccgcgcgcac gacaccctgc agggcgaggg attggaaaat gccctcagcg acctggatcc
     2941 gcgttcgcgt cgcatcgttc aggcgcgctg gttgcaggac gaaggtggcg caaccttgca
     3001 cgatttggcg aacgaatttg gcgtttcggc cgaaaggatc cggcaaatcg aagtcgccgc
     3061 cttcaaaaag atgcgaagtt cgctggctgc ctgagcaata aagacacgat tggatacaaa
     3121 gccgcgctaa aaacgtcgaa ctaattaatg cctggccgga ctaaattttc acaagggctg
     3181 gctttcggtg cgactgcatt ccggcggctc ccttatcgtc cacttcggct tctcctcttc
     3241 ccctcctttt tgaagtggat gttcgacagg gcgcctagcc aggcgccctt tttttcgcct
     3301 gcattacgct aaattaaaaa aaaagttatc ctaagcccgg tgaacagctt tcgggattga
     3361 tcatgcaggt tgggcatttg gtatttggat tggcgggact gcttgcgctg gtgtctttta
     3421 tgcctccgct tgcgggccgc cttcatttgc cacattccgt gctgcttgcg attgtcggat
     3481 tcctgctcgg cctgctcatc cacattcgtg gatgggcgcc gtcggtcatg tcggattttc
     3541 tcgattcgct gcagcgtttc gaaatttcgt ccgaagcttt cctgtttatc ttcctgccgg
     3601 tccttttgtt cgagatgtcg ctggcgatga atgtgcgccg gttgctcgac gatattggct
     3661 cgattctgat gatggcggtc gtggcggtgg tcgtttgcac ggttgccgtc ggtttttcca
     3721 ttagtgccgt gtcggattat ggcctggttg tatgcctgat gctgggagcc atcgtggcga
     3781 cgaccgatcc ggtggccgtt gtgggcgtgt t
//
LOCUS       DATCXU010000216         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_108361,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000216 DATCXU010000000
VERSION     DATCXU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108361"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..628
                     /locus_tag="VIR04_04865"
                     /pseudo
     CDS             1..628
                     /locus_tag="VIR04_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089436.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
     gene            complement(648..1412)
                     /locus_tag="VIR04_04870"
     CDS             complement(648..1412)
                     /locus_tag="VIR04_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY9460110.1"
                     /translation="MPFISAARPVYVGCAGWGLSSTVAAHFPAKGSHLERYAQVLSSV
                     EINSSFYRAHQPKTYARWAASVPDAFRFCVKIPRTITHELRLREADAAMHTFMDQVSS
                     LGDKLGCLLVQLPPSLALNRPEALSFFSTLRGLARVRVVCEPRHISWFTPAAADLMKD
                     AGIACVRAHPSPISGAEPAGDPDPLYIRLHGAPRIYYSAYNDAFIDAVAVRIAIARRS
                     NRQAWCIFDNTARGEAIPNALTLMERLRVMGELATP"
     gene            complement(1420..>2209)
                     /locus_tag="VIR04_04875"
     CDS             complement(1420..>2209)
                     /locus_tag="VIR04_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9460111.1"
                     /translation="FGLRQSLDTVGAFLGPLLAVGLMLLWADDFRAVFWVAVIPGVLA
                     VLLLLFGVQEPERESRQAHKRINPIRRENLRRLDPSYWRVVGIGAIFTLARFSEAFLV
                     LRAQQSGIPIALVPLVMVAMNVIYALTAYPFGKLSDRMSHTKLLALGLGVLILADLVL
                     AADAHWTVVLLGVALWGVHMGMTQGLLAAMVANTAPADLRGTAYGFFNLISGLAMLLA
                     SVLAGLLWDQLGAAFTFYAGAVFCVIALFMLLWVAIHNGKNSKS"
BASE COUNT          503 a          682 c          611 g          413 t
ORIGIN      
        1 cggcgcgagc ctgttggcgt cggccggtgt tgcaggcctg gttgccggca ttgccgcccg
       61 tccggttctg ggcaatttca ttgccggcct gcagatcgcc tttacgcagc ccatccgcat
      121 cgatgacgtg cttatcgtgg aagacgaatg ggggcgcgtc gaagaaatca ctgccgcgtt
      181 cgtcgttttg cgtatctggg atgagcgccg cctgatcata ccgctgcaat ggttcatcga
      241 aaaccctttc caaaactgga cgcatcgttc agcgagtctg ttgggcaccg tctttctgta
      301 gctcgatttt tcggtgccgc ttgacccggt ccgccgggaa ttcgagcagt tgtgcaaatc
      361 gtccgccgcg tgggacggcc gcgtgagagt agtccaggtc accgacactt cggaacgctc
      421 catgcaggtg cgctttctgg tgagcgccgg cgattccggg aaggccttcg acctgcgctg
      481 cttcctgcgc gagaacctga tcgcctacat cgtcacccac tatcctggcg gcctgccccg
      541 cgtgcgcgcc gacgtcgatg ccgacggaca cgcagccctc ccagaaacgc gtccgcaggc
      601 ggaggaaggc agcagtcccg cgccctgatg ggcaacacag gcttcagtca aggtgtggca
      661 agctcaccca tcacccgcaa tcgttccatc aacgtcagcg cattgggaat ggcctcacca
      721 cgcgcggtat tgtcgaaaat gcaccaggcc tgccgattgg accggcgcgc aatggcaatg
      781 cgcacggcta cagcgtcgat gaatgcgtcg ttatatgccg aatagtaaat acgaggcgca
      841 ccatgcagac ggatatacag gggatcagga tcgccggccg gttcagcgcc ggatatcggc
      901 gacgggtgcg cgcggacgca agcgataccc gcgtctttca tcaggtctgc agccgccgga
      961 gtaaaccagc taatatggcg cggctcacat actacacgca cgcgcgcaag gccgcgcagt
     1021 gtcgaaaaaa agctcagtgc ctctggcctg ttcaacgcca gactgggtgg aagctgcacc
     1081 agcaggcacc caagtttgtc gcccagcgaa gagacctggt ccatgaacgt atgcatggct
     1141 gcgtccgctt cgcgcagcct tagttcgtgc gtaatggtgc gcggaatctt gacacaaaat
     1201 cggaatgcat cgggtacgct cgcagcccac cgggcgtagg tttttggttg atgcgcgcga
     1261 tagaacgatg aattgatttc caccgacgaa agtacctgag cgtaccgctc aagatggctg
     1321 cctttggccg gaaaatgtgc agctaccgtc gaggacaggc cccaaccggc gcaccccacg
     1381 taaaccggtc tcgcggcaga aataaacggc atgtcgtttt caactctttg aattctttcc
     1441 attgtgaatc gccacccaca acaacatgaa gagcgctatt acacaaaata cggcaccggc
     1501 ataaaacgtg aatgctgcgc ccagttggtc ccacagcaaa ccggccagca cacttgccaa
     1561 caacatggcc aaaccgctga taagattaaa aaatccatac gcagtccccc gcagatcggc
     1621 cggcgcggta ttggcgacca tggcggccag cagcccttgc gtcatgccca tatggactcc
     1681 ccaaagcgcg actcctaata gcacgacggt ccaatgcgcg tcggcagcga gcaccagatc
     1741 cgcaaggatc aacactccca agcccagcgc caggagcttc gtgtggctca tccggtccga
     1801 taatttgccg aagggatagg ccgtaagtgc ataaatcaca ttcatcgcca ccatcactag
     1861 cgggactagc gcgatcggaa taccgctttg ctgcgcgcgc aggaccaaaa atgcttcgct
     1921 aaaacgagct aatgtgaata ttgcgccaat gccaacaacc cgccagtaag acggatccag
     1981 gcgccgcaag ttttcccgac gtattgggtt gatccgcttg tgggcttgac ggctttctct
     2041 ttctggttct tgtaccccga ataacaatag caataccgcc agcacacccg gaatcacggc
     2101 cacccagaac acagcgcgaa aatcatcagc ccataacagc atcaagccca ccgccaacaa
     2161 ggggccaagg aacgctccga ccgtatcgag ggactggcgc aagccaaag
//
LOCUS       DATCXU010000217         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_590104,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000217 DATCXU010000000
VERSION     DATCXU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590104"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VIR04_04880"
     CDS             complement(<1..660)
                     /locus_tag="VIR04_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY9460112.1"
                     /translation="MKTFRKRLSSLNADHRRIAQGAARVAFFLLLGKFAGAAKEMAVA
                     YRYGVSNAVDAYQFTMTLANWLPLTIVGALSIVLIPILVRIRYAAPDDRARFLGELQA
                     WMMTAGAVLAVAIYVAWPYVLDFAGQGLNAQARVMSQELTFAFAPAALFTLVTGISAA
                     RLRAHERHINTLLDSVPAMVILVWVMLAATVTDVGPLMWGTLVGYLIQSVWLLWLAAR
                     AD"
     gene            complement(662..1687)
                     /locus_tag="VIR04_04885"
     CDS             complement(662..1687)
                     /locus_tag="VIR04_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9460113.1"
                     /translation="MSTRYQEVIANLRAEPQSWLVTGCAGFIGSNILETLLSLDQKVV
                     GLDNFATGYQHNLDEVKKIVSAGQWERFTFHEGDIRDLATCRMAMHGIDHVLHEAALG
                     SVPRSLKDPIATNDVNIAGFLNVLVAARDAQVQSFVYAASSSTYGDHQALPKVEDQIG
                     RPLSPYAVTKYVNELYADVFSRAYGFGSIGLRYFNVFGKRQDPDGAYAAVIPKWVSAM
                     IRGDQVVVNGDGETSRDFCFVENAVQANILASLAKPEGINQVYNVAYNARTSLNQLFD
                     YLAKTLGDNGIVYDKTPVYADFRAGDVRHSQADISKAKRLLGYEPMHNIVQGLEVAMP
                     WYTQFLR"
     gene            complement(1705..2979)
                     /gene="tviB"
                     /locus_tag="VIR04_04890"
     CDS             complement(1705..2979)
                     /gene="tviB"
                     /locus_tag="VIR04_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HEY9460114.1"
                     /translation="MQIQDVKLAIVGLGYVGLPLAVEFGKRRPVLGFDVNVRRIAELK
                     EGRDHTLEIDSNELAQATHLTYAHDVAQLGQANVFIVSVPTPIDQYKQPDLTPLVRAS
                     ETIGKVLKRGDIVVYESTVYPGATEEDCVPVLERISGLKFNEDFFAGYSPERINPGDK
                     THRVSSIKKITSGSTPEVADLVDTLYREIIKAGTHKAPSIRVAEAAKVIENTQRDVNI
                     ALVNELALIFNKMGIDTQAVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQSIG
                     YHPEIILAGRRLNDSMGGYVVSQLVKAMTKRRIQVQGSRVLVMGLAFKENCPDLRNTR
                     VVDIVRELREYNIEVDVCDPWVDAQEATREYGLELTATPQAGAYDGIILAVAHDQFAS
                     MGAAAIRALGKSEHILYDLKYVLRADESDLRL"
     gene            complement(3011..4150)
                     /locus_tag="VIR04_04895"
     CDS             complement(3011..4150)
                     /locus_tag="VIR04_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9460115.1"
                     /translation="MTPPHLWDSFTWLYICIVAFTVGGLIVASERWHGVFTDDTDFNK
                     PQASHSRATPRVGGLAVVAGSLAGLLVLGPNNMTLTWLWPVLFIAAMPVFVAGLLEDI
                     TKDIGSGKRLLAAFASAAIAWWLLGGVTRVGFPAIDWVLSFWPISLLFTMVAVGGCTH
                     ALNIIDGMNGLAGMVAVLMAVSLGLVALQVQDIPIFLIAAALASAILGFLVWNFPFGR
                     VFLGDGGAYFVGFILAELAVQLVVRNPGVSPFYALAVLFYPVFETLFSIWRRRFKRGV
                     PVDQPDALHLHQLVFRRLVRVTFSRDRRHAVPALCNAMTSPYLWVLTLIGLVPATIWW
                     DNVWALCASMALFSAVYIWLYTRLVSWRRPAWLLLPSMGRSRRDK"
     gene            complement(4557..5237)
                     /gene="coq7"
                     /locus_tag="VIR04_04900"
     CDS             complement(4557..5237)
                     /gene="coq7"
                     /locus_tag="VIR04_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY9460116.1"
                     /translation="MVHPDSHPGLAAFSPRLSRVDSVLSEAGRALQVLFGAARAARPN
                     PAGSPEANAPALSTSDQKHAAALMRVNHVGEVCAQALYRGQAIFCRDAATQDLFRRAA
                     GEEIDHLAWCRDRIGELHSRPSLLNPLWYTGSFSLGLMASYASRPKNLGFMAETESQV
                     EQHLSQHLVRLPAADNRSRRIIEQMREDEIVHRVTAETHGAQVLPRPVRLVMRGMAKI
                     MTTVAYRI"
     gene            complement(5311..5940)
                     /locus_tag="VIR04_04905"
     CDS             complement(5311..5940)
                     /locus_tag="VIR04_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460117.1"
                     /translation="MMGPRFFCCGWSKAKRCALGSALLLVTAVVFRDALTSSMALHMV
                     VHIPGILVAGVLAGYALLARFGTPVKGTAGRLWRQYEKYNAYGVPGLLLATLLGAYWM
                     IPKALDQVLASAPADVLKFVVLFGAGIIMADSLKRSNNVIKLFFVGNFCWMMAVVGMM
                     YQQDTTRLCNFYLLNDQQYAGMGLVILSIALPVLWLLVEIKSVRRFLLR"
     gene            complement(5937..>6306)
                     /locus_tag="VIR04_04910"
     CDS             complement(5937..>6306)
                     /locus_tag="VIR04_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY9460118.1"
                     /translation="SLIRERGLAEKIRLISISFDPADTPDQLARYARAMRADPAIWGF
                     AGIPSARARDVLLNTFGIVRVAAPYGQFEHNAAYHVVTANGFLARVVDYADPLAALDF
                     AVSQSTHPAGAALASLVRSQ"
BASE COUNT         1575 a         1896 c         1624 g         1211 t
ORIGIN      
        1 atcggctctg gccgccagcc acaacagcca gaccgactgg atcagatagc caaccaatgt
       61 gccccacatc agcggcccca catcggtgac cgtggccgcc agcatgaccc acaccagaat
      121 caccatggca ggcacactat cgagcagcgt atttatatgc cgttcatgcg cccgcaagcg
      181 cgcggcgctg attccggtca ccagggtaaa tagcgctgcc ggcgcaaacg caaacgtcaa
      241 ttcctggctc atgacgcgag cctgggcgtt caggccctga ccggcaaaat ccagcacgta
      301 aggccaggcg acgtaaattg ccacagccag caccgcgccg gcagtcatca tccacgcttg
      361 cagttcgccc aaaaatcggg cgcggtcgtc cggcgcggca taacgtattc gaaccagaat
      421 cggaatcagg acgatagaca aagcgccgac tatcgtcagc ggcaaccaat tcgccagcgt
      481 catggtgaac tgataggcat cgacagcgtt gcttacgcca tatcgatacg ctaccgccat
      541 ctccttcgct gcgccggcga attttcccaa caaaagaaaa aaagcaacgc gggccgcgcc
      601 ctgcgcaatc cgccgatgat cggcgttaag gctggacaac cgcttgcgga aggttttcaa
      661 gtcaacgcaa gaattgcgta taccagggca tcgcgacttc aaggccttgc acaatattgt
      721 gcatgggctc gtaacccagc aaacgtttgg ccttgctgat gtccgcctga gagtggcgca
      781 catcgcccgc ccgaaaatca gcgtaaaccg gcgtcttgtc gtagacgatc ccgttatcgc
      841 ccagcgtctt cgccaaataa tcgaataact ggttcaaact ggtgcgggcg ttataggcca
      901 cgttataaac ctgattgatc ccttcgggct tggccagcga cgccaaaata ttggcctgca
      961 cagcgttttc aacaaaacaa aaatcgcggc tggtttcgcc gtcgccgttg acgacaacct
     1021 ggtcgccgcg aatcatggcg gacacccact tgggaataac cgccgcatac gcgccatcag
     1081 gatcctggcg cttgccgaag acattgaagt aacgcaagcc gatgctgcca aacccgtacg
     1141 cccgggagaa cacatcggcg tacagttcgt tcacgtactt cgtgaccgca tagggagaca
     1201 acggccggcc gatttgatct tccactttcg gcagggcctg atgatcgccg taagtggaac
     1261 tggatgccgc atacacgaac gactgcacct gcgcatcgcg cgccgctacc agcacattca
     1321 aaaaaccggc aatattgacg tcgttggtcg caattgggtc tttcagggaa cgcggcactg
     1381 aacccagcgc ggcctcgtgc aaaacatgat cgataccgtg catcgccatg cggcatgtgg
     1441 ccagatcgcg gatgtcgcct tcgtgaaacg tgaacctttc ccactgcccg gccgagacaa
     1501 ttttttttac ttcatccaga ttgtgttgat atccggtcgc gaaattatcg aggccgacca
     1561 ctttctgatc aagcgacagc agggtttcca aaatgttgga accaataaaa ccggcgcaac
     1621 ccgtcaccag ccagctttgg ggttcagcgc gtaaattcgc gatcacttct tgataacgag
     1681 tgctcatata agaccttcca cagattacag gcgcaaatcg gattcgtcgg cccgcaatac
     1741 atattttaga tcgtacagaa tatgctcgct tttgcccaag gcgcgaattg cggctgcgcc
     1801 catggaagca aactggtcgt gcgcaacggc caaaataatg ccgtcataag cgcccgcttg
     1861 cggcgtggca gtcaattcca gaccatactc gcgcgtggct tcttgcgcat cgacccacgg
     1921 atcgcataca tctacttcga tgttgtattc gcgtaattca cgcacaatgt ccaccacgcg
     1981 ggtattgcgc aaatcgggac agttttcttt gaaggccagc cccatcacca gaactcgcga
     2041 accctggacc tgtatccggc gttttgtcat cgccttgacc aactgcgaca ccacgtaccc
     2101 gcccatggaa tcattaagtc ttcggccggc cagaataatt tcagggtgat agcctattga
     2161 ttgcgccttg tgagtcaggt aatacggatc gacgccgatg cagtgccctc caacaagccc
     2221 gggcctgaac ggtaaaaaat tccacttggt gccagccgcc tccaggacgg cctgtgtgtc
     2281 aatgcccatt ttgttgaaaa ttaacgccag ctcattcacc agggcaatat tcacgtcccg
     2341 ctgcgtgttt tcaatgacct tggccgcctc cgccacacgg atggatggcg ccttgtgtgt
     2401 gcctgccttg ataatttcac gatagagcgt gtcgaccagg tcagccactt cgggggtaga
     2461 tccggatgtg atttttttaa tcgacgagac gcgatgcgtc ttgtcgcctg gattgatgcg
     2521 ctcgggactg tagccggcaa aaaaatcctc gttgaatttc aagcccgaaa tacgctcaag
     2581 caccggcacg cagtcttcct cggtggcacc ggggtaaaca gtggactcgt aaacaacgat
     2641 atcgccgcgc ttgagcactt tccctatggt ttcactggct cgaaccagtg gagtcaggtc
     2701 aggctgtttg tattgatcga tcggagtcgg cacactgacg ataaacacat tggcctgtcc
     2761 caactgcgcc acgtcgtgcg cataagtcag gtgagtcgcc tgggcaagtt cattcgaatc
     2821 gatctccagc gtgtggtcgc ggccttcttt taattccgca atacgacgca cattgacatc
     2881 gaaccccaat accgggcgcc ttttcccgaa ctcgacagcc agcggcaggc ccacatagcc
     2941 caatccaact atcgccagtt tcacgtcttg aatttgcaac ttgaatctcc cttgtcacca
     3001 aattaaaaga ttatttgtcc cgacggctgc gccccatcga cggcaacaac agccacgccg
     3061 gccttcgcca ggacaccagt ctggtgtaga gccagatata cactgcggaa aacagcgcca
     3121 tgctggcgca caaagcccat acgttatccc accaaatggt cgctggcact agtccaatca
     3181 acgtaagaac ccacaaatac ggggacgtca tggcattgca aagcgccggc accgcatggc
     3241 ggcgatccct gctgaatgta acgcgcacca aacgccggaa aacgagctga tgcaaatgca
     3301 gggcgtcggg ctggtcgacc ggcacaccgc gcttgaaacg ccgccgccag atcgaaaaca
     3361 aggtctcaaa cactgggtag aacagcacgg ccagtgcgta aaacggcgaa acacccgggt
     3421 tccggacaac gagctgcacg gcaagctccg ccagaataaa cccgacgaag taggcgccgc
     3481 catcccccaa aaagacacgt ccaaacggaa aattccagac cagaaacccc agaatggccg
     3541 aagccagcgc tgcggcgatc aaaaaaatgg ggatgtcctg aacctgcaag gcaacgagcc
     3601 ccagcgatac cgccatcaag acagcgacca tgcctgccag cccgttcatg ccatcaatga
     3661 tattcaaggc gtgggtgcac ccccccaccg caaccatggt gaacaacaat gagatgggcc
     3721 aaaaagataa aacccagtcg atggctggaa agcccacacg ggtcacaccg cccagcagcc
     3781 accacgcaat cgcggccgac gcaaacgctg ccagcaaacg cttgcccgat ccgatgtcct
     3841 tggtgatgtc ttcaagtaaa cccgcgacaa aaaccggcat ggcggcaata aacaaaaccg
     3901 gccacaacca ggtcaatgtc atattattgg ggcccagcac cagcaaccct gccagactgc
     3961 cggccaccac agccaggccg cctacgcgtg gcgtggcgcg cgaatgagaa gcctgcggct
     4021 tgttaaaatc ggtatcatcg gtaaatacac cgtgccaccg ttcggacgcc acaatcaagc
     4081 ctccgaccgt aaaagccaca atgcatatat aaagccaggt gaaagagtcc caaaggtgcg
     4141 ggggtgtcac gaaatctccg gtgtcaacat accgttagca ttatcggacg gtcccgtaac
     4201 gacatgcgga gccgacaagg aattagtgta actaaaaatc agccggcaag gcggattcca
     4261 tcgtcggcgc ttcgcggata aaaaaaagcc cgggatcttg cgatctcggg ctaaatccac
     4321 caaaggagga gggtggagga gacaacctgg gcatgtcgcg ctacaaaagc caattagctt
     4381 gagccaagtg attgttaagg ggttttccct gctggggttt tcccgctgca atcatgtttc
     4441 gacatccgat aaccaaattg tatagcacta gtttgtgcat tgcaaggtga atgatcaaaa
     4501 aagttgctta aacgcgcatt cgttgctttt tcgcaaacct tgcagtcgct tacagtttat
     4561 atacgatacg caaccgtagt cattatcttc gccatgcccc gcataacaag ccgtaccggc
     4621 cggggtaaaa cctgagcgcc gtgtgtctca gccgtgacgc ggtgcacaat ttcatcctcg
     4681 cgcatctgct caataatgcg ccgcgagcgg ttgtcggccg ccggcaaccg gaccaaatgc
     4741 tggctcaagt gctgctcgac ctgtgattct gtttcggcca tgaagccgag gtttttgggc
     4801 cggctggcat aactggccat taatcccaga gaaaatgagc ccgtatacca taacggattg
     4861 agcaaactgg ggcggctgtg caattccccg atgcgatcgc ggcaccacgc cagatgatcg
     4921 atttcctcgc cggcagcgcg ccggaacaga tcttgtgttg ctgcatcgcg gcaaaaaatt
     4981 gcctggcctc gatacaaagc ctgtgcgcaa acctcgccaa cgtgattaac gcgcatcagc
     5041 gccgctgcgt gtttttgatc ggaagtggac agggctggcg cgttcgcttc gggagagccg
     5101 gctgggttcg ggcgggccgc acgggcggcc ccaaacaata cctgcaaggc acgcccggcc
     5161 tcggataaaa cgctatcgac acgactgagg cgaggcgaaa acgccgccag acccggatga
     5221 ctatctggat gaaccatcga taaatcccaa aatatatggc ccctttcatg cggcccgacg
     5281 ccccggccgc cgaccgccgc gacgaagcat ttagcgcagc agaaaacgcc gcaccgactt
     5341 gatttccacc aacaaccaaa gaacaggaag cgctatcgat aatattacca agcccatccc
     5401 cgcatattgc tgatcattta ataaataaaa gttgcacaag cgtgttgtgt cttgctgata
     5461 catcattcct acgacggcca tcatccagca aaaatttccc acaaaaaaca atttgatcac
     5521 gttgttggat cgtttcaggg agtcggccat tattattccg gcgccaaaca agacgacaaa
     5581 cttcaatacg tcggcgggcg ccgaggcaag cacttgatcg agcgccttcg gaatcatcca
     5641 gtatgccccc agtagcgtcg ccagcaaaag gcctgggacg ccatacgcat tgtatttttc
     5701 atactggcgc cacagccggc cagccgttcc ctttacaggt gtgccaaagc gcgccagcag
     5761 ggcgtaaccc gcaagcacgc ccgccaccag aatacccggg atgtgaacaa ccatgtgcag
     5821 ggccatggac gacgtcagcg catcccggaa aacaacggcc gtcactaaaa gcaatgcact
     5881 gccaagcgcg caccgctttg ccttggacca gccgcaacaa aaaaatcgcg ggcccatcat
     5941 tgtgatctga ccagtgaagc cagcgcggcg ccagccggat gcgtgctttg cgacacagcg
     6001 aaatccagag cggccagcgg gtcggcatag tcgacaacac gcgccagaaa cccattggcc
     6061 gtgaccacgt gataagccgc gttgtgctca aactgcccgt atggagcggc cacccgaaca
     6121 attccaaacg tgttcaacag cacgtcgcgc gcacgcgcac tgggtatgcc ggcaaacccc
     6181 cagatggcgg ggtcggcgcg catcgcccga gcgtatcgtg caagttgatc gggcgtgtcg
     6241 gcgggatcga aactgatgct gatcaggcga atcttttcag ccagtccgcg ctcgcgtatc
     6301 aggctt
//
LOCUS       DATCXU010000218         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_5951, whole
            genome shotgun sequence.
ACCESSION   DATCXU010000218 DATCXU010000000
VERSION     DATCXU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5951"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..943
                     /locus_tag="VIR04_04915"
     CDS             362..943
                     /locus_tag="VIR04_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY9460119.1"
                     /translation="MQHDRDFGTTGSTASSVQGYSSLAKLLHWMIVLLVLAQFVVAIL
                     MPDIGRNTVPGPLINLHFSLGVLILVVMAIRFMFRLLYPVPLDMPGSPLWERWTAHAT
                     HLVFYFILLTGPFLGWASASAHKLPVDVFGIFVLPAIAPAKADWALAAGDIHAYMMWM
                     LLVLIALHVAGALYHRFFRHDGVLRRMLPVRWN"
     gene            1069..>2307
                     /locus_tag="VIR04_04920"
     CDS             1069..>2307
                     /locus_tag="VIR04_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY9460120.1"
                     /translation="MTNVQAKKVGELKDGFEGEVVLPGDGAYESARKIWNAMIDKRPA
                     LVARCSTTSDIVRSVNFARENKFVLAVRGGGHNIAGSAICDDGIVVDLSQMKTANVRP
                     DARRVTIEGGATLADLDAATQAHGLAVPLGINSTTGVAGLTLGGGFGWLSRKYGMTVD
                     NLESAEVVTATGEVVRASATQQPDLFWALRGGGGNFGVVTHFEFRLHPVGPDLLSGLI
                     VYPFSEAKSVLQRYRDFIAKAPDELSVWVVLRKAPPLPFLPPEVHGKEIIALAILYAE
                     DPKRGQALIEPLRTFGTVLGEHVGVQPYTAWQQAFDPLLAPGARNYWKSHNFSTIKDG
                     LIDVVMEYVAKLPSPQCEIFFGAIGGATTRPAPDSAAYAHRDAQFVMNVHGRWEDAAD
                     DKRCIAWARDYFKASAPFASG"
BASE COUNT          438 a          632 c          685 g          552 t
ORIGIN      
        1 tgcccgggcc ctgacgagat gcatgatgtc gggcatgtgg tttcaccttt ttgcggcgga
       61 ctccaatcct agtgttgatg gcgatgaggc cgcagtccca tcaagattac gtattacatt
      121 attcgtacca gatcgtctat agcagtttcg taaacgaatg tgtagtggtg cagtcatggt
      181 ggcggactac acggcgaatg aacaccgctt gataaagcgt tgtcagcttg ccgcggctcg
      241 ttatttcgcg gtcgaggcct tggtgcaacc ggccttataa tcttggcgag gcaccgcttt
      301 tttgtgatgc gcaacgcttt gcaaacgctg aatgtgtttc aacaacgcca agagaacaca
      361 gatgcaacat gaccgcgatt tcggaacgac cggatcaacc gcttccagcg ttcaagggta
      421 cagctcgctg gcgaaactgc tgcactggat gatcgtcctt ttggtgttgg cgcagtttgt
      481 cgttgcgatc ctgatgcccg acatcggacg caataccgtg ccgggaccgc ttattaatct
      541 gcacttctca ttgggtgttt tgattctggt tgtgatggcg atccgtttta tgttccggct
      601 gctttatccg gtgccgctgg atatgccggg atcgccgctg tgggagcgct ggacggctca
      661 cgcgacccat cttgttttct atttcatttt gctgacgggg ccctttttgg gttgggcttc
      721 ggcgtctgct cacaagcttc ccgttgatgt gttcggcatt ttcgtcttgc ctgccattgc
      781 tcccgcgaaa gctgactggg cgcttgccgc cggcgatatt cacgcataca tgatgtggat
      841 gttgctggtg ttgattgcgt tgcatgtggc cggtgcgctt taccatcgct ttttccgcca
      901 cgatggtgtg ctgcggcgca tgctgccggt tcgatggaac tgacgcgttg caattgtgtc
      961 agccggtaaa aaacgaagct tgcaagggta cgtattacat atgccgagcg tatagtaagc
     1021 ggacgaacgc atcgcgcaat tggcgacaac gcactatggg agaaaaccat gacaaacgtg
     1081 caagccaaaa aagtcgggga gctgaaggac ggatttgaag gagaagtagt tcttccaggc
     1141 gatggcgctt atgaaagcgc gcgcaaaata tggaacgcaa tgatcgacaa gcgacccgca
     1201 ctcgttgctc gttgttccac cacctcggac atcgttcgca gtgtgaactt cgccagggaa
     1261 aacaagtttg tgctggctgt tcgcggcggc ggccacaaca ttgcgggcag cgccatttgc
     1321 gacgatggaa tagtcgtcga cttgtcgcaa atgaagactg cgaacgtgcg gcccgacgct
     1381 cgccgggtca ccatcgaagg tggcgccacg cttgctgatc tggacgcggc tacccaggct
     1441 catgggcttg ccgtaccgct gggcatcaac tccaccacag gcgtcgcggg attgacgcta
     1501 ggcggcggct tcggctggct tagccgcaag tacggcatga ctgttgacaa cctggagtcc
     1561 gccgaggtcg tgaccgcgac tggcgaggta gtgcgcgcca gcgcgaccca gcaacctgat
     1621 cttttttggg cgctgcgcgg cggtggcggc aatttcggcg tggtgaccca ttttgaattc
     1681 cggcttcatc ccgtcggccc tgatttactc agcggactaa tcgtgtaccc gttttccgag
     1741 gcaaagtcag tattgcagcg gtaccgcgat ttcatcgcca aagcgcccga cgaactttct
     1801 gtgtgggttg ttttgcgcaa ggcgccgccg ctgccgtttc ttccaccaga ggttcacggc
     1861 aaggagatca ttgcgcttgc gattctctac gcggaggacc caaagcgcgg ccaggcgctt
     1921 atcgagccct tgcgcacgtt cggtactgta ctgggcgagc atgttggcgt tcagccctat
     1981 accgcctggc aacaggcctt tgatccactg cttgctcccg gcgcgcgcaa ctactggaag
     2041 tcgcacaatt tttcgacaat caaggatggg ctgattgacg tcgtcatgga gtacgtcgcg
     2101 aagcttccgt cgcctcagtg cgagattttt ttcggtgcga tcggcggcgc tactaccagg
     2161 cccgcccccg attccgctgc gtatgcccac cgcgacgcgc agttcgtcat gaacgttcat
     2221 ggccgatggg aggacgccgc tgacgacaag cgctgcatag cctgggcgcg cgattacttc
     2281 aaagcgtcgg cgccgtttgc cagcggc
//
LOCUS       DATCXU010000219         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_414090,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000219 DATCXU010000000
VERSION     DATCXU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414090"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..624
                     /locus_tag="VIR04_04925"
     CDS             142..624
                     /locus_tag="VIR04_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460121.1"
                     /translation="MKHFKILLLATALVFPLANAVSQTAPHEHEAVVHGSMELNAGQK
                     WATDAPLRQGMTKIRTAVLTALPAAQSGSLTPAQYDVFADDVNSQVVYIVQNCKLEPK
                     ADAQLHVLLGDITTGVSVAKGEKQGEARSQGVIKVAESVNNYGKYFDHADLQPIAVPH
                     "
     gene            827..1132
                     /locus_tag="VIR04_04930"
     CDS             827..1132
                     /locus_tag="VIR04_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY9460122.1"
                     /translation="MKAMRTAAGAACRLMKALSNPDRLLLLCQMTQGEKRVGELEALV
                     GIGQPTLSQQLGVLREEGLVSTRRDGKNIYYQVADPRTLAVMEVLYAQFCGAQKGKK"
     gene            1132..1563
                     /locus_tag="VIR04_04935"
     CDS             1132..1563
                     /locus_tag="VIR04_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY9460123.1"
                     /translation="MSIDFVHFTPWASLIGGMLIGLSAAVLILVNGRILGVSGIVGGL
                     LRPKPGDWGWRLSFLLGVLAAPALWAAFLGPAASRIEAGWATLLIAGLLVGLGTRLGS
                     GCTSGHGVCGISRLSPRSMAATALFMAAGFIMVYVIRHALA"
     gene            1576..2028
                     /locus_tag="VIR04_04940"
     CDS             1576..2028
                     /locus_tag="VIR04_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY9460124.1"
                     /translation="MQRLVEFSAGLVFGLGLILSGMADPARVLGFLDLTGLWDPSLAF
                     VMGGAILVGLFAFRIARKRARTLLGGALHLPGATAIDKRLVLGSLIFGAGWGLAGFCP
                     GPAIVSLGAGHPKAVVFVVAMLAGMLLSPWTSSMLRCFQQALPRKGQD"
     gene            2030..>2832
                     /locus_tag="VIR04_04945"
     CDS             2030..>2832
                     /locus_tag="VIR04_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9460125.1"
                     /translation="MVTSKTDSAPDPKRETNDRNARQQDKPSSSSLQDVCNLLQLNRT
                     LTLQTEGRSELRFQHRRFKTGEFIYRMGQTFGVLYIVRFGFLKTVLHNTQGDERILSF
                     PMKSDLIGFDGIYRDRYTSDTVALTDCDLIAIPFKELLTPGHNNGDLEQMIYMATSRE
                     IALERGGMGLSITVKSEARVARFLAMQSRRYTNLGYSSRNFLLPMTRRDLGSYLGLTL
                     ETISRSLSALATAGIIAINRRDIEILKPELLILPPDFLHLPERQASQAS"
BASE COUNT          636 a          791 c          765 g          640 t
ORIGIN      
        1 ccatgttcac cggacgcaag cgccccttgc aaactgaggc ccatttaact tcggaagcga
       61 aaattgacgc agatcaataa gctccacagg tatcgcctgt aagatcaatt cgtatcaatc
      121 aaactcaata cgggatcaat catgaaacat ttcaaaattc tgctgctggc cactgcgctg
      181 gtctttccgt tagccaacgc ggtcagccag accgccccac acgagcatga ggccgtagtg
      241 catggctcga tggagttaaa tgctggccaa aaatgggcga ccgatgcgcc gttgcgccaa
      301 ggcatgacta aaatccggac agccgtttta accgcattgc cggccgccca aagcggtagc
      361 cttacacccg cgcaatacga tgttttcgcg gacgacgtca attcgcaggt cgtttacatt
      421 gtgcaaaatt gcaaacttga gcctaaagcc gacgcgcagc ttcacgttct tttgggcgac
      481 atcacgacag gtgtcagtgt cgcaaaagga gaaaagcaag gcgaagcgag atcgcaaggc
      541 gtaatcaaag tagcagagtc cgtaaataac tatggcaagt attttgacca tgcggacctg
      601 cagcccatcg cggtgccgca ttaaccgcgt ggtcgggcaa gccgatttgg aatcgtagcc
      661 aagcacacag tcgatcacca cgcactttta cggtggagct caccgcgctc cttcggcaat
      721 cgattaatgt acaactatta tattaaatga tattatattt ttaaatatat tgttgtcaca
      781 ttaaaagaag gactgaatca gcgtgacgcc gcaagctgtt tttgacatga aggccatgcg
      841 cacagccgcg ggcgccgcat gccggctgat gaaggcgctc tcaaacccgg acaggctgct
      901 tttgctatgc caaatgacgc aaggcgaaaa acgtgtgggc gagcttgaag cattggtcgg
      961 catcgggcag cccacgctat ctcagcaact tggggtgttg cgcgaagaag gacttgtcag
     1021 cacacggcgc gatgggaaaa atatttatta ccaggttgcc gacccgcgaa cgcttgccgt
     1081 catggaagtg ctctatgcgc aattttgcgg cgctcaaaaa ggaaaaaaat aatgtcgatc
     1141 gacttcgtcc atttcacacc atgggcctcc ttgataggcg gcatgctgat cggcctgtcg
     1201 gcggcagtgc tcatattggt taatgggcgc attcttggcg tcagcggcat tgtaggaggg
     1261 ctgctgcggc caaaacccgg cgactggggt tggcgccttt catttttgct cggggtattg
     1321 gccgcgccgg cgttgtgggc agcattcctg ggccctgccg cttcacggat tgaagccggc
     1381 tgggccacat tactgatcgc cggtctgctt gtggggctgg gaacccgact tggctcgggc
     1441 tgcaccagtg gccatggcgt gtgcggcatc tcgcgcctgt caccgcgctc catggcagcc
     1501 actgcgctgt tcatggcagc cggttttatc atggtgtacg tcattcgtca tgccttagcc
     1561 tgaccaggga aaaacatgca acgactcgtc gaattttcgg caggcctggt ttttggcctg
     1621 ggcctgattc tctccggtat ggccgacccg gccagagttc tgggctttct ggatttaacc
     1681 gggctatggg atccgtcgtt ggcttttgtc atgggcggcg ccattctggt ggggcttttc
     1741 gccttcagaa tagcccgcaa acgtgcccgc actcttcttg gcggcgcatt acacctgccc
     1801 ggcgccacgg ccattgacaa gcgtttggtg ctcggcagcc tgattttcgg cgccggctgg
     1861 gggctggctg gtttctgccc tggcccagcc atcgtatcgc tgggcgccgg ccacccgaag
     1921 gcagtcgtgt ttgtggtggc catgctggcg ggcatgcttc tttcaccatg gacatcatct
     1981 atgctacgct gtttccaaca ggctctcccc agaaaggggc aggattaaca tggtcacttc
     2041 aaaaactgat tcagcgccag atccgaaacg tgaaaccaat gaccgcaatg cgcggcaaca
     2101 ggacaagcct tcttcctcct cgcttcaaga cgtctgcaac ctgctgcagc ttaatcgaac
     2161 gttaacgcta cagacagaag gcagatcaga gcttcgcttc cagcaccgcc gattcaaaac
     2221 cggtgaattc atctatcgca tgggacagac tttcggcgtg ctgtacatcg tgcgtttcgg
     2281 cttcctgaag acagtgctgc ataatacgca gggcgatgag cgcatccttt cctttcccat
     2341 gaaaagcgac ctgatcggct tcgacggaat ctaccgcgat cgatacacca gcgacacggt
     2401 ggctttgacc gactgcgatc tgattgcgat acctttcaaa gaattgctta cacccggtca
     2461 caacaatggc gatctggaac aaatgattta tatggcgacc agccgtgaaa tcgcgctgga
     2521 acgcgggggc atgggtctgt caattacggt caaatcagaa gcgcgcgtag ctcgcttcct
     2581 ggccatgcaa tccaggcgct ataccaatct gggatattcc tcgcgcaact ttttgcttcc
     2641 catgacgcga cgcgatctgg gcagctacct tggcctgaca ctcgagacca tcagccgctc
     2701 gctatcggct ttggcgacgg cgggcattat tgccatcaac cgacgcgaca tcgaaatact
     2761 aaaacccgaa ctgttaatac tgccacccga ctttcttcac ttgccggaac ggcaggcaag
     2821 ccaggcaagc ca
//
LOCUS       DATCXU010000220         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_980367,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000220 DATCXU010000000
VERSION     DATCXU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980367"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VIR04_04950"
     CDS             complement(<1..703)
                     /locus_tag="VIR04_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY9460126.1"
                     /translation="MTANLSTEELAAWVRLSLEPGLGLARARQLLTAIGLPQDIYASS
                     AGALSKIIPCDLAQQFHRPLADTLGATLKRTLEWSSQPDHHVLTLADPAYPRALLDIH
                     DPPLLLYINGQPDRLSRPIIAMVGARNATQGGIDNALAFARYLSDQGWCIASGLAQGI
                     DAAAHEGALRAGPKGGSTVAILGTGIDIVYPPKNHELAHRIAQHGALVSEFPLGTRVM
                     PYQFPKRNRIVAGLAR"
     gene            825..1337
                     /gene="def"
                     /locus_tag="VIR04_04955"
     CDS             825..1337
                     /gene="def"
                     /locus_tag="VIR04_04955"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY9460127.1"
                     /translation="MALLPILQYPDPRLHKVAKPVQKVDDRIRKLVRDMAETMYAAPG
                     VGLAATQIDVHERVVVIDVSEDSNQLHVLINPEILWKSDETQFCEEGCLSVPGVYDEV
                     ERAARVRVRALNEQGQSYEFDAEDLLAVCVQHELDHLLGKVFIEYLSPLKQNRIRTRL
                     RKQEREAQRV"
     gene            1348..>2136
                     /gene="fmt"
                     /locus_tag="VIR04_04960"
     CDS             1348..>2136
                     /gene="fmt"
                     /locus_tag="VIR04_04960"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY9460128.1"
                     /translation="MGPIIFAGTPEFARVAFSALLAAGHTISLVLTQPDRPAGRGLKL
                     MPSAVKQAALAANIPVCQPRSLRLEGKYPDDARAARDALLATAPELIVVAAYGLILPR
                     WLLLAPKYGCLNIHASLLPRWRGAAPIQRALEAGDAQTGVTVMQMNEGLDTGDIVLTR
                     SLAIAPDWNAARLHDELAQLGARSIIQVIGNLEHGKLVAKPQAAQGVSYAAKLTKTEA
                     ALDLSQPATVLERRIRAFNPAPGATLRLPGLDEPVKVWAAQALGQ"
BASE COUNT          422 a          597 c          676 g          441 t
ORIGIN      
        1 cccgcgccag accggccact atacggttgc gctttggaaa ctgatagggc atgacgcgcg
       61 tacctagcgg aaattccgat actagcgcgc cgtgttgcgc aatccggtgc gcgagctcgt
      121 gatttttcgg cggatatacg atatcgattc cggtccccag gatggcgacg gtgctgccgc
      181 ccttcgggcc ggcgcgcaaa gccccttcat gcgccgccgc gtcgatgccc tgagccaggc
      241 cgctggcgat gcaccagccc tgatcggaca gatagcgggc aaaagccagc gcgttgtcga
      301 ttccgccttg tgtggcgttg cgcgccccca ccattgcaat gatgggccgc gacaggcgat
      361 ctggctggcc gttgatgtat agcaacagag gaggatcgtg aatatcaagc agcgcgcggg
      421 ggtaggccgg atccgccagc gtcaggacat ggtgatcggg ctgactgctc cactcgagcg
      481 tgcgcttgag ggttgctccc agcgtatcgg ccagcggcct gtggaactgt tgcgctaggt
      541 cgcaggggat gatcttggac aaggctccgg cggagctggc gtaaatgtct tgcggcaggc
      601 cgatcgccgt gagtaattgg cgggcccgag ccaggcccag gccgggttcg agcgaaaggc
      661 gtacccaagc ggctaattct tcggttgata ggttggcagt catacgctaa gtgtggcaca
      721 gcgcaagcga atagcgttac tcgagcgaca gtaaaagtca cgttatttca actttgccgt
      781 atcttgtttt attataggag gttagaggca caattggact tgttatggcg ttactcccta
      841 ttcttcaata ccctgatccg cgcctgcata aagtggccaa gccggttcaa aaggttgacg
      901 atcgcattcg caaattggtg cgcgacatgg ctgaaaccat gtacgcggcg ccgggtgtcg
      961 gtttggccgc aacccaaatc gacgtgcatg aacgggtggt cgttatcgac gtttccgaag
     1021 acagcaacca attgcacgtt ctcatcaatc ccgaaattct ctggaagagc gacgagaccc
     1081 aattttgcga agagggctgc ttatccgtgc ctggcgtata cgacgaagtt gaacgggcgg
     1141 cgcgggtaag ggtgcgcgca ctcaatgaac aggggcaatc ctacgaattc gatgccgagg
     1201 atttgctggc cgtgtgcgtg cagcacgagc tcgatcattt attgggcaag gtgtttatcg
     1261 aatacttgtc gcccttgaag caaaatcgca tcagaacccg gcttcgcaaa caagagcgcg
     1321 aggcgcagcg cgtataaggg gtacggcttg ggtcctatca tttttgccgg cacccccgaa
     1381 ttcgcccgcg tggcattcag cgctctgcta gcggcggggc atacgatcag cctggtttta
     1441 actcaacccg atcgccctgc ggggcgcggc cttaaactca tgcccagcgc ggtcaagcag
     1501 gcagcgcttg cggcgaatat tcccgtttgc cagcctcgca gtttgcgtct ggaaggcaag
     1561 tatcccgacg atgcccgcgc cgcgcgcgac gcattgctgg cgacggctcc cgagctgatt
     1621 gtggtggcgg cttacggcct gatcttgccc cgatggctgc tgttggcgcc caaatacgga
     1681 tgtttgaaca ttcatgccag cttgttgccg cgctggcgcg gcgcggcgcc tattcagcgc
     1741 gccctggaag ccggcgatgc gcaaactggc gtcaccgtca tgcaaatgaa tgaagggctg
     1801 gataccggcg acattgtgct gacgcggtct ttggcgatcg cgccggattg gaatgcggcg
     1861 cgtttgcacg atgagctggc gcaactggga gcacggtcaa tcatccaagt tattggcaat
     1921 ctcgaacacg gcaagctggt cgcgaaacct caggccgcgc aaggcgtcag ttatgcggcc
     1981 aagttgacca aaaccgaggc tgcgcttgat ttatcccagc ccgcgaccgt tttggagcgc
     2041 cgaattcggg cgtttaaccc ggcgccgggc gcgacgctaa ggttaccggg gctggatgaa
     2101 cctgttaagg tatgggcggc gcaggcgctt ggccag
//
LOCUS       DATCXU010000221         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_893736,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000221 DATCXU010000000
VERSION     DATCXU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893736"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VIR04_04965"
     CDS             complement(<1..1077)
                     /locus_tag="VIR04_04965"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY9460129.1"
                     /translation="MAQTLYDKLWDDHIVFQEPDGTCLLYIDRHLVHEVTSPQAFEGL
                     ALADRKPWRVGANLAVSDHNVPTTARDQGIKDPVSRLQVDTLDSNCEKYDITQFRMND
                     VRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGALGALAFGIGTSEVEHVLATQTLLM
                     KKSKNMLIKIEGELPFGCTAKDLVLFVIGKIGTAGGSGYAIEFAGSTVCALSIEARMT
                     MCNMAIEAGARSGMVAVDEKTLDYCRGRPYAPSGALWDQAVQYWRTLHSDEGAKFDRV
                     VEIDARQIMPQVTWGTSPEMVLSVDGKVPDPDKEKDDGRRKGMERALEYMGLTPNTPL
                     VDIRLDKIFIGSCTNARIEDLRAAA"
     gene            complement(1207..2271)
                     /gene="aroC"
                     /locus_tag="VIR04_04970"
     CDS             complement(1207..2271)
                     /gene="aroC"
                     /locus_tag="VIR04_04970"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY9460130.1"
                     /translation="MSGNTLGKLFCVTNFGESHGPAIGAVVDGCPPGLALTPADIQLE
                     LDRRRPGTSRHVTQRKEADLVEILSGVYEGVTTGTPIGLLIRNTDARSKDYNNIADTF
                     RPGHADYTYWRKFGQRDPRGGGRSSARLTAPTVAAGAIAKKWLAQNFGIKIRGYMSQL
                     GPIPIPFVSWDEVSKNAFYAPNAGIVPQLEAFMDQLRRDGDSIGARIEVVAENPPAGW
                     GEPLYDRLDADIAHVMMGLNAVKGVSIGSGFDCVAQRGSEHGDEMTPGGFSSNHAGGI
                     LGGISSGQAITVSIAIKPTSSIRIERQSVNRAGEPVSVQTTGRHDPCVGIRATPIVEA
                     MLALVLIDHALRQRAQCLLP"
BASE COUNT          509 a          700 c          622 g          496 t
ORIGIN      
        1 ggctgccgcc cgcaagtctt caatgcgagc gttcgtgcag gaaccaataa agatcttgtc
       61 cagccggatg tcgaccaatg gcgtattagg cgtcaggccc atatattcca atgctcgctc
      121 catacccttg cggcgtccgt cgtccttttc tttatcagga tcgggaacct tgccatcgac
      181 cgataaaacc atttcaggcg aggtgcccca tgtgacttgc ggcatgatct gtctggcgtc
      241 gatttcaacc acacgatcga actttgctcc ctcgtcggaa tgcaaagtcc gccaatactg
      301 aacggcctga tcccaaagag cgccgcttgg cgcatagggc cggccgcgac agtaatccaa
      361 tgttttttcg tcaaccgcca ccatgcccga acgggcgccg gcctcgattg ccatattgca
      421 catagtcatg cgcgcctcaa ttgaaagcgc gcacacggtg ctgccagcaa actcgatggc
      481 ataccccgat ccgccggccg tgccgatttt cccgataacg aacagcacca gatctttggc
      541 tgtgcaaccg aacggcagct cgccttcgat cttgatcagc atgtttttgc tttttttcat
      601 cagcaacgtt tgcgttgcca gcacatgctc tacttcggat gtcccgatac cgaacgccag
      661 tgcgcccagc gccccatgcg tgctggtgtg ggaatcgccg cacacaatcg tcatgcccgg
      721 caaggtggcg ccctgttcgg ggccgataat atgcacaatg ccctggcgca catcgttcat
      781 gcggaactgg gtaatgtcgt atttttcgca attgctgtct agcgtgtcga cctgcaaacg
      841 cgagacagga tctttaatcc cctgatcgcg cgccgtggtc ggcacattgt ggtcagagac
      901 agcaagattg gctcccacgc gccagggctt gcgatcagcc agcgccaggc cttcgaatgc
      961 ctgcggacta gttacttcgt gcaccagatg gcgatcgata tacagcaagc atgtcccgtc
     1021 gggctcctga aacacgatgt gatcgtccca gagtttgtcg tataaagttt gggccataat
     1081 ccaatgctcg cagaaagaag tcaattaaac accgccgcgg gccttgcgcc cgtggcattc
     1141 gtccattatg ccagcattgg aacctagtac aagtacagtc tttatactag tattatatgt
     1201 acctgcctag ggcaggaggc attgggcacg ttgccgcaag gcatgatcga tcagcaccag
     1261 cgccagcatt gcctcgacaa tgggtgtggc acgtatgcct acacaagggt cgtgccggcc
     1321 cgtggtttgc accgacaccg gttcaccggc ccgattcacc gactggcgct cgatgcgaat
     1381 gcttgaggta ggcttgattg cgatcgacac ggtaatcgcc tggcccgagg aaattccccc
     1441 caatatgccg cccgcgtggt tgcttgaaaa cccgcccggc gtcatttcgt cgccgtgctc
     1501 ggagccgcgc tgggccacgc aatcaaagcc cgagccgatc gaaacccctt tgaccgcatt
     1561 caggcccatc atgacgtggg cgatatcggc gtcaagccga tcgtacaaag gctcgcccca
     1621 acccgctggc gggttttcgg cgaccacctc aatgcgagcg ccaattgaat cgccatcgcg
     1681 gcgcaactga tccataaagg cctcgagttg cggaacaatg ccggcattgg gcgcgtaaaa
     1741 cgcgttcttc gagacttcgt cccacgaaac gaaaggaatc ggaatgggtc ccaactggct
     1801 catataacca cgaattttta tgccaaaatt ttgcgccagc cattttttcg cgatggcccc
     1861 agcggccacc gtgggcgccg tcaaacgggc tgacgaacgt cctccgccgc gcggatcgcg
     1921 ctgaccgaat ttgcgccaat aggtgtaatc ggcatgtccg ggacgaaaag tatcggcaat
     1981 attgttgtaa tccttgctgc gggcatcggt attgcggatg agcaggccaa taggcgtgcc
     2041 ggtcgtcacc ccctcataga cccccgacaa aatttcaacc agatccgcct ctttgcgctg
     2101 agtgacatga cgcgacgttc ccggccgacg acgatccagt tcgagttgaa tatcggccgg
     2161 cgtcagcgcc aaccccggag ggcagccgtc aaccacggcg ccgatggcgg gaccatgcga
     2221 ctcgccgaaa ttggtgacgc aaaagagctt gccaagtgta ttgccggaca tatgatgtct
     2281 ttataagtag tggtgcgaaa aagataccat aaacctgacc gttacga
//
LOCUS       DATCXU010000222         7019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_228888,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000222 DATCXU010000000
VERSION     DATCXU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228888"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VIR04_04975"
     CDS             <1..776
                     /locus_tag="VIR04_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY9460131.1"
                     /translation="LPAPLRRVAAASLTCLPTSAWDKALKVLQPVLPAKSRQSATGEK
                     LHKLAELLSAGNKEAFYRQLVSHWKQPGDVVIGSTEHSTLLSDPQAWPQGLSFEHWMM
                     AMDAQTYMTDDILVKVDRAAMANSLEVRVPMLDHRVVELAWRMPQDFKIRDGKGKWLL
                     REVLYRHVPKELMERPKKGFSIPLANWLRGPLRNWAEALLNETRLRKEGYFRPYPIRQ
                     AWAQHLSGKRDNSRKLWCVLMFQAWLNQQADATPPVPPL"
     gene            complement(773..1753)
                     /locus_tag="VIR04_04980"
     CDS             complement(773..1753)
                     /locus_tag="VIR04_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020699685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460132.1"
                     /translation="MSLTKKALFLSLTVSLLGLGAVAPSYAASYPDRPITLVVPFPPG
                     GGSDSVARGLARGMSERLGVSVVVDNKAGGGTIIGTQHVVRSKPDGYTLFWGSTPTAI
                     NPSVYKDLSYDTLKDLTTISDVGQQPLVLVVNSSSPAKTLKDLIDEGKKDPGKLTYGS
                     SGVGGSPHLATALLLHEAGVDAVHVPYKGSAPAVQGVLANQTSFVFDTVILTLPFIKA
                     GRLNALAQTSAKRSPLLPNVPTVAEAGYPGYEITSWYSVLAPAATPKAIITKLNKVIN
                     EVLADPKIASTFTAQGFQMAGDTPEQAQEHLKQEIKKWSKAVEYSGVRAN"
     gene            complement(1871..3955)
                     /locus_tag="VIR04_04985"
     CDS             complement(1871..3955)
                     /locus_tag="VIR04_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY9460133.1"
                     /translation="MLKNLLNPGSIAVIGASEDQAKFGGRLLKNLLRHGYAGTIYPIN
                     PTKNELFGLKAYSSLGATPTVADAFVVALPAHKIKEQVETAAKIGIKLGIIISAGFSD
                     AGESGRISEAEIVEIARRHGMRLMGPNCLGIVNAQSKIVLCSSEVMDRDTLPVRPIGF
                     VSQSGALMTTSFDRAWSDGGGFTYGFSVGNQADLELCDFVDFLIDDEATTVICTYIEG
                     LKDPKRWLASARKARQAGKPIVVVKAGRSAAGRQAAFSHTASVTGDNAVFTAVCREEG
                     VLVVDDINTMLTVANVLATQPRRPLNTIAIMTPSGGGGALAADALSDTDLKLASFSEQ
                     TQSSLAGLYPSGQADNPIDFGTRLGSDPLQTAQKTLQALHSDPNIDGILFVVTMAPRG
                     WLLSLVDAQAELNGGQNKPVIFAFDAGTTVDPVRERLAELGIPYANTTSEAVKALGYV
                     HKFATGVADMQQASRPIECDSSVLKGLEGQLDEQQTKKILSQYGIRVNAGACAANIDE
                     AVKIARNVGFPVVMKIVSPDIIHKSDVGGVIVRLADEADVRAAHATMLANVADKAPRA
                     RIEGVFVQSMVEGGLELILGARNDAQFGPIIVYGAGGILVELLPDKVIARCPLSITDA
                     IRGLQSLSIWPILSGYRGQSLAVDALVDAIVRLSWLALDLEDRTFEIDINPLIAGTSA
                     AVAVDGRMQIAL"
     gene            4114..5295
                     /locus_tag="VIR04_04990"
     CDS             4114..5295
                     /locus_tag="VIR04_04990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9460134.1"
                     /translation="MKELSLSDLPLAGVKVVEFCSVAAGPFCAMLLADMGADVVKIEP
                     PGGDALRQWPPITNGFSENFASLNRNKESIELNLKDDSDRELARQLVHEADVVVENNR
                     PGVMKRLGLDFSSFSQHLPHLIYCSISAYGQTGPRSDEGGFDLTMQAAAGVMSVTGEP
                     DGVPVKCGVPISDFSSGLYAAFAISSVLARVRAGGAGAHIDAPMFGCTLGVGALQTSE
                     FFGNGRSPGRLGSAHPRNAPYQAFQAADGYFAIAAGNQKLWVAVCRGVDLPELENDAR
                     FSTTSKRAANQVELKTILEECFMKKPVDDWIRVFKDAGVPHARINDFHAALDDPQVDH
                     MHWVRNIELPGGGATRTFASPLLLNGAAFPIRRNPPGLGEHTEAIRAHFKATACKGEP
                     S"
     gene            5292..5876
                     /locus_tag="VIR04_04995"
     CDS             5292..5876
                     /locus_tag="VIR04_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEY9460135.1"
                     /translation="MKNGKLLSFVTALTHLVDQADLTEVEVLGQAKLLMSSLVAQDDW
                     LPESMAVPDSEHYRQNLLYGDPLDRFSLISFVWGPAQRTPIHNHTVWGVIGMLRGAET
                     QQPYRITPKGLQPAGPDQILKPGEVACVSPTIGDIHTVRNAFDDRVSISIHVYGGNIG
                     RIERTTYAPATGLPTRFVSGYSNPLLPNLWSRED"
     gene            5884..6621
                     /locus_tag="VIR04_05000"
     CDS             5884..6621
                     /locus_tag="VIR04_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9460136.1"
                     /translation="MYKTLTIQRSDRVVHINLDRSTAGNSLSQEMVGELQEIVDQSYE
                     DGTALLVLGAQGRHFCTGFDLSQIDSETDDGLLARFVRIELLLQAIHTAPFTTLALGH
                     GRIMGAGADLFAACELRWLVGSANFCFPGAAFGLVLGTGRLARLIGAERAREWVGSGR
                     RIEPPEALACGLVTAEILPERLSEELERVAQRSQNLDPLTYKRIRAASGHDPVSQARD
                     LYALAQSAARPGVKERIQRYRAGIKKP"
     gene            6742..>7019
                     /locus_tag="VIR04_05005"
     CDS             6742..>7019
                     /locus_tag="VIR04_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9460137.1"
                     /translation="MARQTKQSSAAELNTAQPSITASETTPSQSGSLTKGLAILEVLA
                     TSDKPLGVTDIALIMDTAKSGVHRLLQTLRSTGWVSKTPEGRYECTLRL"
BASE COUNT         1639 a         2020 c         1830 g         1530 t
ORIGIN      
        1 tgctgcccgc acccttgcgg cgcgtcgcgg ccgccagcct gacctgcttg ccgacatcgg
       61 catgggacaa ggccctcaag gtgctgcagc ccgttctgcc ggccaaatcc agacaatcgg
      121 cgacaggtga aaagctgcat aagctggccg agcttttatc agcaggcaac aaggaagcct
      181 tctaccgtca attggtcagc cattggaagc agcccggtga tgtggtgatc ggctcgacag
      241 agcacagcac cttgctgagt gatccgcaag catggccgca ggggctaagc tttgaacact
      301 ggatgatggc aatggacgcc cagacttata tgacggacga catcctggtc aaagtcgatc
      361 gcgccgccat ggccaacagc ctggaggttc gagtcccgat gctcgatcat cgcgtggtcg
      421 aactcgcctg gcgcatgccg caagatttta aaatccgcga tggcaaagga aaatggctgt
      481 tgcgtgaagt gctttaccgg catgtgccga aagaactcat ggaacgcccc aagaaggggt
      541 tttccatacc attggcgaat tggctgcgcg gcccattgcg caattgggcc gaggctctgc
      601 ttaacgaaac ccgactgcgc aaggaaggct atttccgccc ctaccccatc agacaggcct
      661 gggcacagca tttaagcggc aagcgcgaca attcaagaaa gctatggtgc gtattgatgt
      721 tccaggcgtg gctaaatcag caggcggacg ccactccacc cgttccgccc ctctaattcg
      781 cgcgtacgcc cgagtactca acggcttttg accatttttt gatttcttgc ttcagatgct
      841 cttgtgcttg ttcgggggtg tctccggcca tctgaaagcc ctgtgccgta aaggttgatg
      901 cgatcttcgg gtcggctaat acttcgttga tcaccttatt cagcttggtg ataatagctt
      961 tgggcgtggc cgctggggcc agaactgaat accacgacgt aatttcgtag ccagggtaac
     1021 cggcttccgc aactgtcggc acattcggca atagcggcga acgcttcgcg gatgtttgag
     1081 ccaaagcgtt cagtctgcca gctttaataa agggcaaggt caaaataacg gtgtcgaaaa
     1141 caaaagaagt ctgattggcc aacacgccct gcaccgcggg agcacttccc ttatacggaa
     1201 catggacagc atcaaccccc gcctcgtgca aaaggagcgc cgtggctaaa tggggcgacc
     1261 cgcccactcc agatgaaccg taagtcaatt tacccggatc tttcttgcct tcgtcaatca
     1321 agtccttaag tgtttttgcg ggactggatg aattcaccac caaaaccaac ggctgttgtc
     1381 ccacatccga aatggtcgtg aggtctttca atgtgtcgta cgaaagatct ttatagaccg
     1441 aaggattgat cgcagtggga gtcgaccccc aaaaaagcgt gtatccgtcg ggcttgctgc
     1501 gcacgacgtg ctgggtgccg attatcgtgc cgccgcccgc cttgttatca acaacaaccg
     1561 atacaccaag ccgttcactc atgccacggg caagtccacg ggcgacgctg tcagagccgc
     1621 cacccggggg gaacggaacc accagcgtga tgggcctgtc ggggtagctt gccgcgtagc
     1681 ttggagcaac cgcacccaag ccaagcagcg atacggtcag ggataaaaat agggcttttt
     1741 tcgtcaggct catgatttgt ctccgccagt ttggttcaat ctttagtgtg ttccgttata
     1801 cggaaatacg ttccaaattt aataatatcg ataaagaagg actttagtca ataactaaat
     1861 atccactttt ttacaatgcg atctgcatac ggccatccac agcaaccgct gcagacgtac
     1921 ccgcaataag gggattaatg tctatctcga aggtgcgatc ctccaggtca agcgccagcc
     1981 aactcagccg gacaatagcg tcgaccaacg cgtcgaccgc taatgactgc ccccgatagc
     2041 cggacaaaat cggccatatc gacagagact gcaatccacg tatggcgtcc gtgatcgaca
     2101 atggacaacg agcgatcacc ttgtctggca acagctccac cagtatgcct ccagcgccat
     2161 aaacgatgat cggtccgaac tgtgcatcgt ttcgcgcgcc cagaattaat tcaaggccgc
     2221 cttcaaccat cgactgtacg aatacgcctt caatgcgcgc ccggggcgcc ttgtcggcca
     2281 cgttggcaag catggtggca tgggccgccc ttacgtcagc ctcgtctgcg agacgaacaa
     2341 taaccccgcc cacatccgac ttgtgaataa tatcgggcga aacgattttc atgacaaccg
     2401 gaaagccaac gttccgggca attttgacgg cttcatcgat attggccgca cacgccccgg
     2461 cgtttaccct gattccatac tgacttaata ttttcttggt ttgctgttca tcgagttgac
     2521 cttccaatcc cttcaatacg ctgctatcgc attcgattgg acggcttgcc tgctgcatgt
     2581 cagctacgcc tgtggcaaat ttgtgaacat agccgagcgc cttcaccgcc tctgatgttg
     2641 tatttgcgta cggaatcccc aactcagcca gacgttctct gacagggtcg actgtggttc
     2701 cggcatcgaa ggcgaagatc accggcttat tctgtccacc gttgagctct gcctgcgcat
     2761 ccacaaggct cagcaaccag ccacgcggcg ccattgtcac aacaaacagg atcccatcta
     2821 tattcggatc gctgtgcaat gcctgcaagg ttttctgcgc cgtctgcaaa ggatcggaac
     2881 ccaggcgggt tccaaagtcg atcggattgt cggcctgacc agagggataa agcccggcca
     2941 acgatgattg tgtctgctcg ctgaaactcg ccaatttcaa gtcagtatct gacaaggcat
     3001 ccgcagccaa ggctccgcct cctccggacg gcgtcatgat ggcaattgtg tttaatggcc
     3061 gtcgcggctg tgttgccagt acattggcaa cggtaagcat cgtattgata tcgtcgacga
     3121 caagcacacc ctcttcacga caaaccgcgg taaaaacggc attgtcgccc gtgacgctgg
     3181 ccgtgtggga aaaagcggct tgccttcccg cagccgaacg cccggctttc accaccacta
     3241 taggcttgcc tgcctgacgg gctttgcgtg cggacgccaa ccatcgcttc gggtctttaa
     3301 ggccttcgat atacgtgcaa ataaccgtgg tcgcttcgtc atcgattaaa aaatcgacaa
     3361 aatcgcaaag ctccagatca gcctgattgc ctacagaaaa gccatacgtg aagccgcccc
     3421 cgtccgacca ggcccgatca aatgaagtcg tcattaacgc accgctttgg cttacaaaac
     3481 caattggcct gactggcaga gtgtcccgat ccatgacttc cgaagagcac aacactattt
     3541 tgctttgggc atttacgatg cccaggcaat tcggccccat caatcgcatg ccatgacggc
     3601 gtgcaatctc cacgatttcg gcttctgaaa tgcgcccgct ttcgcccgca tcagagaagc
     3661 cggcggaaat aatgattcca agttttatcc ctattttggc cgctgtttcc acctgctctt
     3721 taattttatg ggccggcaaa gcaaccacaa atgcatcggc gacggtggga gtcgccccca
     3781 gtgaggaata ggctttcaaa ccgaaaagtt cattttttgt cggattgatg gggtaaatcg
     3841 tccccgcgta accatgccgc agcaggtttt ttaacagcct gccgccaaac ttcgcctgat
     3901 cttccgacgc gccaatcacg gcaatggaac ccggattcaa cagatttttc aacatttcaa
     3961 ctatctccca tcgtcgctat ctgcacgatt catcataacg tgaagtcgcc gccagccaac
     4021 aaaatattca accgttgtca aaggcattgc tcaaaaattt aagttctgta aaatggaaca
     4081 tcaatactat attccagaac ggatgagaaa taaatgaaag agctcagcct tagtgatttg
     4141 cctcttgcag gcgtgaaggt cgtcgaattt tgctcggtgg cggccggacc attctgcgca
     4201 atgttgcttg ccgatatggg agccgacgtt gtcaagatag aaccacctgg cggcgacgct
     4261 ttaagacagt ggccacccat tacaaatgga tttagcgaaa actttgcctc gctaaataga
     4321 aacaaggaat ccatcgagct caatctgaag gacgacagcg accgggagct ggctcgtcag
     4381 ttagtgcatg aagccgacgt ggtggttgaa aataatcgtc caggtgtcat gaagcgattg
     4441 ggtctggatt tttccagctt ttcccagcat ttgccacact tgatttattg ctctatttca
     4501 gcgtatggcc agaccggtcc gcgctcggac gaaggcggtt tcgacctgac gatgcaagca
     4561 gccgccggag tgatgagtgt gacgggcgaa cctgatggag tccccgtaaa gtgcggcgta
     4621 ccaatttccg atttttcctc tggtctttat gcggcattcg ccatttcctc ggtattggcg
     4681 cgtgtgcggg cgggtggcgc cggcgcccat attgatgccc ccatgtttgg ctgtacgctg
     4741 ggcgtgggag cactgcaaac cagtgaattc tttggaaacg ggcgaagtcc tgggcgactt
     4801 ggctccgcgc acccgcgcaa cgccccttat caggcatttc aggcggcgga tggctatttc
     4861 gccatcgcgg cgggcaatca aaaactttgg gtcgccgttt gccggggggt cgatttaccc
     4921 gaactggaaa atgacgcccg attctcaacg accagcaagc gcgcggccaa tcaggtagag
     4981 ctgaaaacta ttctggaaga gtgttttatg aaaaagcctg tcgatgattg gatcagagtg
     5041 ttcaaagacg caggcgtgcc ccatgcacgc atcaatgact tccacgccgc gttggacgac
     5101 ccccaggtgg atcacatgca ttgggtgcgc aatattgaac tgcccggcgg tggcgcaacc
     5161 cgaacctttg cttcgccgct gcttctgaat ggcgcagctt ttccgatccg gcgcaatccc
     5221 cccggtctgg gcgaacacac cgaggccatt cgcgcccatt tcaaagcgac agcatgcaaa
     5281 ggtgaaccat catgaagaac ggcaagttat taagtttcgt aacggcgtta actcatttgg
     5341 tggaccaagc cgatctgact gaagttgaag tcttgggcca agccaagctt ctaatgagca
     5401 gcttggtggc gcaagacgac tggctccccg aatcaatggc cgtacctgat tccgagcact
     5461 atcgccaaaa tctgttatat ggcgacccgc tcgatcgctt ttcactgatc agcttcgttt
     5521 ggggtccggc acaacgaacc ccgattcaca accacaccgt ctggggcgtc atcggtatgc
     5581 tgcgcggcgc ggaaacgcag caaccgtatc ggatcacccc caaaggactt caaccagccg
     5641 gtcccgacca gatcctgaag ccaggcgaag ttgcctgtgt gtcgcccacc atcggcgaca
     5701 tccacaccgt ccgcaatgct tttgatgacc gggtatcgat cagtatccac gtatacgggg
     5761 gaaacatcgg acgcatagaa cgcactacgt atgcgcccgc gacggggtta ccgacacgct
     5821 tcgtgtccgg ctactcgaac cccttgctac ccaatctttg gtccagggaa gactaaacag
     5881 accatgtata aaaccttgac gattcagcgc agtgatagag tagtgcatat caatcttgat
     5941 cgatccaccg cgggcaatag tttgtcgcaa gagatggttg gcgagctgca ggagatcgtc
     6001 gaccagagct acgaagatgg cactgccttg ctggtgctgg gagcgcaagg ccgacatttt
     6061 tgcacagggt tcgacctatc gcagatagac agcgaaaccg atgacggact gctggcccgc
     6121 tttgtccgca tcgagctgct gcttcaggcc attcacaccg cgccatttac gacgctggcc
     6181 cttgggcatg gccgaatcat gggagcaggc gccgaccttt ttgctgcatg cgaactacgc
     6241 tggctcgtcg gcagcgccaa tttttgcttc ccgggagccg cgtttggcct ggtgctgggc
     6301 accggacgac tggcccgcct gattggcgcc gagcgcgcgc gtgaatgggt cggctccggg
     6361 cgtcgcatcg agccccccga agcactggcc tgcggtcttg tcacagcgga aatcctgccg
     6421 gaacggctgt cggaagaatt ggaacgcgta gcacaacgct cgcaaaatct cgatccattg
     6481 acatacaagc gcattcgcgc ggcatcggga cacgacccgg tatcgcaagc ccgggacttg
     6541 tatgctctgg cgcaatcggc tgcacggccg ggcgtcaaag aacgtataca gcgctatcgc
     6601 gcaggcataa aaaagccata gcgcaccgat ttcagtccca aacgcggcct ggtgcactac
     6661 aatcgccgca gacttcccgg tctcatcgaa atgcgcgcgc cgtgaacggc gcatccatat
     6721 tgtgaaccac accgacttat tatggctcgt caaactaaac aatcatccgc cgctgaactc
     6781 aacacagcgc agccatccat cacggcttca gaaaccacgc catcacagtc cggttccctt
     6841 acaaaaggcc tggccattct ggaagtgctg gccacaagcg acaagccgct cggagtaacc
     6901 gacattgccc taatcatgga taccgccaaa agcggtgttc accgattgct gcaaaccctg
     6961 cgcagtaccg gatgggtctc aaaaactccc gaaggccgat acgaatgcac gcttcgtct
//
LOCUS       DATCXU010000223         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_810230,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000223 DATCXU010000000
VERSION     DATCXU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810230"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..303)
                     /gene="thiS"
                     /locus_tag="VIR04_05010"
     CDS             complement(82..303)
                     /gene="thiS"
                     /locus_tag="VIR04_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY9460138.1"
                     /translation="MSTIRITVNGTEHTVAQALTLAQLLDHLKITADEPAVPVASAVN
                     GQYVAKRARAGLVLNDQDAVTTFEPITGG"
     gene            complement(300..1379)
                     /locus_tag="VIR04_05015"
     CDS             complement(300..1379)
                     /locus_tag="VIR04_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9460139.1"
                     /translation="MNIGIAGAGLLGRLLTYTLTRQNHHVSIFDPASSPTARGAAGWT
                     AAGMLSPTSELEAADLNVFDLGLRSLKRWAEIIPQLRQPVSLDHAGSLLLAHRHDAGA
                     AQRIIQLLTQKAPPDYRPQAISLQQLAHYEPSVQGPSHAWLLPHEGHLNTVQGMQALY
                     ASATTADWHWSEPVTELAAHTLVTAQRRYTFDWVFDVRGLGEKSAVAGEHANEISCQN
                     VRGVRGEIFWLHAPGVKLNRPIRLLHPRHRVYLVPRVDDIIVVGATEIESEDRSPVSL
                     RSTVELLAAAHSVIPALAEARVVHSETNLRPALIDNLPRIEHQSGLTRINGLFRHGWM
                     ISPALVDDALSRVFSETPSLQRVDL"
     gene            complement(1455..>2324)
                     /locus_tag="VIR04_05020"
     CDS             complement(1455..>2324)
                     /locus_tag="VIR04_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY9460140.1"
                     /translation="AGIPAGVLNIIAADSQRSIEIGKVLCASPIVKKVTFTGSTAVGR
                     ILMQQSAATVKKLSLELGGHAPFIVFDDADIDAAVEGAMISKYRNTGQTCVCANRFYV
                     HEAVYDLFVEKLAAKVGSLKVGDGFTDAVTQGPLIDDAALAKVQEHLKDAVNKGASVV
                     TGGKGHALGGRFFQPTVLSDITEDMLCMREETFGPLAPIVKFTDEDHVVELANNTDYG
                     LASYFYSRDVGRIFRVAEALEYGMVGVNTGLISNEVAPFGGVKQSGLGREGSIYGMDD
                     FMEMKYVCLGGIV"
BASE COUNT          464 a          659 c          686 g          515 t
ORIGIN      
        1 gaataagcgg cgacggataa ccggcggtgc ccaaaagaaa gcggctttcc agtgtggcgc
       61 ctgcaattga aaacgacatg tttagccccc tgtaatcggt tcgaacgtgg tgacggcgtc
      121 ttgatcgttt aaaaccagcc cggctctggc gcgcttggca acgtattggc cattgacggc
      181 gcttgcaact ggaacagcgg gttcgtccgc cgttattttc aggtgatcaa gcagttgcgc
      241 aagcgtcagc gcttgcgcca cagtgtgttc agtgccgttg acggtaattc ggatggtgct
      301 cacaaatcaa ctcgttgaag tgacggggtt tcagaaaaaa cgcgcgataa agcgtcatcc
      361 accagcgcgg gcgagatcat ccagccatgc cggaagaggc cgttgatgcg cgtcaggccg
      421 ctttggtgct cgatacgtgg aagattgtcg atcaatgccg gacgcagatt ggtttcgctg
      481 tggacgaccc gcgcttctgc cagcgctgga ataacgctgt gcgcggcggc cagcaactcg
      541 accgtgcttc gcaacgagac gggagaccgg tcttcgcttt cgatttccgt ggcgcccacg
      601 acgatgatgt cgtcaacacg cggcaccaga tacacacggt ggcgcgggtg caacaagcgt
      661 attggccggt ttaattttac gccgggggca tgcagccaga atatctcgcc tcgtacacct
      721 cgtacgttct ggcatgaaat ctcattggcg tgctcgcccg cgaccgccga tttttcccca
      781 agaccgcgaa cgtcgaatac ccaatcgaac gtgtagcggc gttgcgcggt gacaagcgtg
      841 tgggcggcaa gttccgtgac gggctcgctc cagtgccagt cggcggtggt cgccgaggcg
      901 tataaagctt gcatgccctg caccgtatta aggtggcctt catggggcaa tagccaggcg
      961 tgcgacggcc cctgcacact gggctcatag tgcgccaact gttgcaacga tatggcctgg
     1021 ggacggtaat ccggcggcgc tttttgcgtc agcaactgaa taattctttg cgctgcgccg
     1081 gcatcgtgcc ggtgcgccaa aagcagactt cccgcgtggt ccagcgacac cggttgccgc
     1141 aattgcggaa taatttcagc ccatcgcttt agcgagcgca aaccaagatc aaatacgttc
     1201 agatcggcgg cttcgagttc cgaggtgggg ctcagcatgc cggcggcagt ccagccagcg
     1261 gcgcctcggg cggtcgggct cgatgcgggg tcgaaaatgc tgacgtggtg gttttgtcgg
     1321 gtgagggtat acgttagcag gcggccgagc aaaccggccc ctgcaatgcc tatgttcatg
     1381 tgcaatgcgc cttatattgc tcaagtatag tttaagtttg ctctatcttg aggcgccggt
     1441 gcaccggatt cttcttatac tatgcccccc agacatacat atttcatttc catgaaatcg
     1501 tccataccgt agatggaccc ttcgcgaccc agtcccgact gtttgacgcc gccaaaaggg
     1561 gcgacctcgt ttgagataag gccggtgttc acgccgacca tgccgtattc cagcgcctcg
     1621 gcgaccctga aaatgcggcc cacgtcgcgg ctgtaaaagt acgatgccag gccataatcg
     1681 gtattgttgg ccagttcgac aacgtgatcc tcgtcggtga atttgacgat gggcgccagc
     1741 ggtccgaatg tttcttcgcg catgcacagc atgtcttcag tgatatccga taaaacagtc
     1801 ggctggaaga agcggccgcc aagcgcgtgg cccttgccgc cggtaacgac actagcccct
     1861 ttgttcaccg cgtctttcag gtgttcttgc actttggcca gcgcggcgtc gtcgatcaac
     1921 ggtccttgcg ttacggcatc ggtaaagcca tcgcctactt tcagactgcc gactttggcc
     1981 gccagctttt caacgaacaa gtcgtaaacc gcttcatgca cgtaaaatcg gttggcgcaa
     2041 acgcacgttt gtcccgtgtt gcggtatttg gaaatcatcg cgccttcgac ggctgcgtcg
     2101 atgtcggcgt cgtcgaacac gatgaacggc gcatggccgc ccaactcgag cgacagtttt
     2161 ttgactgtgg cggcgctttg ttgcatcagg atgcgtccga cggccgttga gccggtgaag
     2221 gtcaccttct ttacaatggg gctggcgcag agcaccttgc ctatctcgat cgagcgctgg
     2281 ctgtcggcgg caataatatt aagcacgccc gccggaatgc ctgc
//
LOCUS       DATCXU010000224         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_918072,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000224 DATCXU010000000
VERSION     DATCXU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918072"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..881)
                     /gene="queE"
                     /locus_tag="VIR04_05025"
     CDS             complement(126..881)
                     /gene="queE"
                     /locus_tag="VIR04_05025"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY9460141.1"
                     /translation="MPTVTLRAAGWFRWKLSSTEPIAPCLPKARGCTMFPVNEVFQTI
                     QGEASYTGTPSVFLRLQGCPVGCPWCDTKHTWHIAPEHAVTISAMLEKQTDAPTYAQM
                     TCQDLVDTVRKFQARHVVITGGEPCSYDLTELTTQLIGLGYSVQIETSGTYPVRVHPR
                     TWVTVSPKIGMPGGLTVLPDALSRANEIKHPVGRERDIDNLRVVLLEANPQALVWLQP
                     LSQSAKATALCIREATANGWRVSIQTHKFLGVR"
     gene            complement(769..1242)
                     /locus_tag="VIR04_05030"
     CDS             complement(769..1242)
                     /locus_tag="VIR04_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY9460142.1"
                     /translation="MTYQSTKTYGHDVGLSTAFRQWTALSHCHFLHGYALAVRFVFEA
                     DELDANNWCIDFGGMKSLKQWLHDTFDHKTVVAQDDPSIEWFREGQRIGTLDLIELPA
                     TGCEKFAEFIYAAAQAWMDANGYSPRCRLVSVEVIEHGANSALFAKSSGVHNVPG"
     gene            1451..3025
                     /locus_tag="VIR04_05035"
     CDS             1451..3025
                     /locus_tag="VIR04_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY9460143.1"
                     /translation="MEWLSDPNMWLGLLTLIVLEIVLGIDNLIFIAILADKLPPHQRD
                     KARLIGLSLALLMRLGLLAIVSWLVTLTTPLFTLGAFSFSARDLILLSGGIFLLVKAT
                     TELHERLEGATHDQGGSRAYAGFAVVVTQILVLDAVFSLDSIITAVGMVDELPVMMAA
                     VVIAMGMMMWASRPLTRFVSAHPTVVVLCLSFLLMIGLSLTAEGLGFHIPKGYLYAAI
                     GFSIMIEFFNQLARRRLMKNEARLPLRDRTADAVLRLMGSKKPPKTGEAAQDARPEME
                     EPAFGPEERNMVSGVLTLGERSIRSIMTPRTEISWINLDDERSVIKSHLRDTPHSFFP
                     VCRGQLDNILGVARAKDLMADMSDGKDLNNLPSLRPPIIIHELAGVLNAMETLKKSRG
                     QLVLVTDEYGAIEGLLTPIDILEAIAGEFPDEDEPPAIRSIGQGRWLVDGGTDLHYLS
                     QVLETEIAEEGGDGFTSVAGLLLDRFGTLPETGATIDLGGLHFEVLKIADRRIATLRV
                     TRVDTQPPAPPLSDQK"
     gene            3022..3465
                     /locus_tag="VIR04_05040"
     CDS             3022..3465
                     /locus_tag="VIR04_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460144.1"
                     /translation="MNSTSTPFKLLGSAAIIVAGLIAAAIARNPARPMVWMVAYLVLV
                     VGVVQYALGVGQAALSKTAPGSATVWGQWLLLNIGHAGVIAGTLAGSFRLLVAGTILY
                     DVAVAWLGLTVRGNQPGMRLIGYRVLIVVMLASSAAGLVLSTLGK"
     gene            complement(3393..3926)
                     /locus_tag="VIR04_05045"
     CDS             complement(3393..3926)
                     /locus_tag="VIR04_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY9460145.1"
                     /translation="MLDAYDRKILAEVQADARIAQSELGERVHLSTAAVNRRLKRLAD
                     DRAIEKYTAVVAPEALGYALTIVAEVEAESVQVDLLDAMKRSFSASPLVQQCYYVTGQ
                     CDFILVITAQSMEQYQELTRELFFQNNNVKRFRTFVSMSRVKVTLDVPIGPAHFPSVD
                     STNPAAELASITTIKTR"
     gene            4093..>4441
                     /locus_tag="VIR04_05050"
     CDS             4093..>4441
                     /locus_tag="VIR04_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460146.1"
                     /translation="MNPSIETRSTPATHGIARPNLIANPLARRCPYPVGHKVLMDVER
                     VAEIAQWLAGWEHICHTPTPLRTLPDLAQSLGIGALRLKDESRRSPIKSFKALGAPIA
                     LIRLILRLYPDRGI"
BASE COUNT          970 a         1268 c         1204 g          999 t
ORIGIN      
        1 acgccctccg tttgtacaag gcactgcgtt caagaataat accgttcctg ccctactcac
       61 cacatcacac attacattaa gtgacaacac cccacttcat cgtcttatat caatgccacg
      121 aagtgtcatc gaacaccaag aaacttatgt gtctgtatgg aaacgcgcca cccgtttgcc
      181 gtcgcttcgc gaatgcaaag cgcggttgcc tttgcgcttt ggcttaatgg ctgaagccag
      241 accagcgcct gtggattcgc ctcgagtaaa acgacacgca ggttgtcgat atcccgctcg
      301 cggccaaccg gatgtttgat ttcgttggca cgcgataaag catcgggcag aacagtcagt
      361 ccgcccggca tgccgatctt ggggcttacc gtcacccacg tccggggatg cacgcgaacc
      421 ggatatgtcc cgctggtctc gatctggact gaatacccaa gcccgataag ctgggtggtg
      481 agctccgtca aatcatagct gcagggctcg ccacctgtta ttacaacatg ccgcgcctga
      541 aatttacgca ccgtgtccac caggtcctgg caggtcatct gcgcgtaggt gggcgcatct
      601 gtttgctttt caagcattgc agaaattgtc acagcgtgtt ctggcgcgat atgccatgtg
      661 tgtttggtat cacaccacgg gcatcctacc gggcagccct gcagtcttag aaatacagag
      721 ggggtgcctg tatacgacgc ctcgccctgt attgtctgga aaacctcgtt aaccgggaac
      781 attgtgcacc cccgagcttt tggcaaacaa ggcgctattg gctccgtgct cgataacttc
      841 caccgaaacc agccggcagc gcggagagta accgttggca tccatccagg cctgggccgc
      901 cgcataaata aattcggcaa atttttcgca accggtggcc ggcagttcga tcaaatccag
      961 cgtgccaata cgctgccctt cccgaaacca ctcaatcgat ggatcgtcct gggccacgac
     1021 tgtcttgtga tcgaaggtat cgtgcagcca ttgtttaagc gatttcatgc ccccgaagtc
     1081 aatacaccag ttgttggcat ccagttcatc ggcctcgaat acaaatcgca cggccaaggc
     1141 atatccgtgc aaaaagtggc aatggctcag cgctgtccat tgacgaaacg ccgttgacaa
     1201 tccgacgtca tggccatagg ttttcgtaga ttgataggtc atcgtaatag ttgattccag
     1261 cgtcccgtac ggtcgaggcg gctctgagtc aattatattc ggtgatcgag cccatttgcc
     1321 ggcctcctga tcaaaacccc gaccgtagca atctgaccta aattcgatag ccatggcgaa
     1381 taaattgccc tactcttctt gagggacgcc gtaataacac agttttacgt actttttacc
     1441 gggatcacat atggaatggc tttcagatcc gaatatgtgg ctaggtctgc tgacactgat
     1501 cgtactagaa atcgtactcg gtattgataa cctgattttc atcgcgatcc tggccgacaa
     1561 actgccgcct catcagcgcg acaaggcaag actgatcggc ttatcgctgg cgctgctcat
     1621 gcggctgggt ttgctcgcga ttgtgtcgtg gctggtgacg ctgaccacgc ccttgttcac
     1681 gctcggggct ttttcgttct cggcacgaga cctgattctg ctttccggcg gcatattcct
     1741 gctggtcaag gcaactaccg agctgcacga gcggctggaa ggcgccaccc atgatcaggg
     1801 cggctccaga gcatatgctg gatttgccgt cgtggtcacg caaatcctgg ttttggatgc
     1861 cgtgttctcg ctggattcga tcattacggc tgtggggatg gttgacgaat tgccggtcat
     1921 gatggccgcc gtggtcatcg ccatgggcat gatgatgtgg gcgtccaggc ctctgacccg
     1981 ctttgtgagc gcgcatccca ccgtcgttgt gctgtgcctg agctttctct tgatgatcgg
     2041 gctaagtctt accgccgaag gactgggctt ccatatcccc aagggttatc tgtacgccgc
     2101 aatcgggttt tccattatga tcgaattttt caaccagctt gcacgacgcc gcctgatgaa
     2161 aaatgaggct cgtctgccat tgcgagaccg cacggccgat gcagtattgc gcctgatggg
     2221 aagcaaaaag ccgcccaaaa cgggcgaggc tgctcaggac gcccgccccg aaatggaaga
     2281 gcctgcgttc ggacccgagg aacgcaatat ggtgagcggc gtgttaacgc ttggtgaacg
     2341 ctcgattcga tccatcatga cgccacgcac agagatttcg tggatcaacc tggatgacga
     2401 acgatcagtg ataaaaagtc atctgcgaga caccccccac agtttcttcc cggtctgccg
     2461 gggccaactg gataacatat tgggtgtggc ccgagccaag gaccttatgg ccgatatgag
     2521 cgacggcaag gatctgaaca atcttccgag cctgcggcca cccattatta ttcacgaact
     2581 ggccggcgtg ttgaacgcaa tggaaacgct gaaaaaatca cgtggtcaat tggtgctggt
     2641 caccgacgag tatggagcaa ttgaaggatt gctcaccccc atcgacatcc tggaggccat
     2701 tgccggcgag tttccggacg aggatgaacc gcccgccatc cggtcgatcg ggcaagggcg
     2761 ctggcttgtc gacggcggga ccgacctgca ctatttgtcc caagtcctgg agaccgaaat
     2821 agccgaagag ggtggcgacg gatttacgtc agtcgccgga ttacttctgg accggtttgg
     2881 gacgttgcct gaaacaggcg caacgataga tctgggcggg ttgcactttg aggtgctgaa
     2941 aatagcggac aggcgcattg ccacgcttcg ggtgacgcgt gtcgataccc agccgccagc
     3001 acctccttta tcggatcaga aatgaattcc acatcgacac cattcaaact gctcggtagc
     3061 gccgccatca tcgtggccgg tctgatcgcc gccgctattg cccggaatcc tgcgcggcct
     3121 atggtctgga tggtggctta tctggtgttg gtggtcggcg tggtgcaata cgcgctgggt
     3181 gtaggccagg cagcgctgtc taaaacggct ccaggctccg ccacggtgtg ggggcaatgg
     3241 ttactgctga acattggtca cgcaggtgtc attgcaggca ctctggcagg aagcttcaga
     3301 ttgctggtgg cgggcacaat actgtatgac gtggctgtgg cctggctggg cctgaccgtt
     3361 cgaggcaatc agccaggaat gcgattgatt ggctatcgcg ttttgatagt cgtgatgctg
     3421 gcaagctcag cggcagggtt ggtgctatcc acgcttggga agtgagcagg cccaatgggc
     3481 acatccagcg taaccttgac ccggctcatg gacacgaagg tcctgaagcg cttgacatta
     3541 ttgttctgga aaaacagctc gcgggtcagt tcctgatact gctccatgct ctgcgccgta
     3601 atgaccagaa tgaagtcgca ttgccccgtc acgtagtagc attgctgcac caacggagaa
     3661 gcgctaaaac tgcgcttcat cgcatcgagc agatcgacct gcacactctc ggcctcgacc
     3721 tcggccacaa tcgtcagtgc atagcccagc gcctcgggcg ccaccactgc cgtgtacttt
     3781 tcaatggccc gatcgtcggc cagacgcttc agccgccggt tcaccgcagc cgtggacagg
     3841 tgcacccgtt cacccagctc gctttgcgca atgcgcgcgt ctgcctgtac ctcggcaagg
     3901 atctttcggt cgtaggcatc gagcatcaat ggctccaata gaaattgttc agctaaatca
     3961 tgggccaatg ggaataaatc ccattcctgc ggcctaaata gaataaaaat ccgtatgaat
     4021 atgaaatatt atttcaatat tagcggtgca tcaacctcat gatgccggac agcaaaaaac
     4081 aggagttcaa tcatgaaccc atctatcgag acacgcagca cacccgccac tcacggaatc
     4141 gcaagaccca accttatcgc caaccctctt gcccgccgct gcccctatcc cgtcgggcac
     4201 aaagtcctaa tggatgtcga gcgcgtcgcc gaaatcgcgc aatggctggc tggctgggag
     4261 cacatctgcc acacacccac gccactgcgc acattgcccg atctggcgca atcgctgggt
     4321 atcggcgcct tgcgcctaaa ggacgaatcc aggcgctcgc ccatcaaaag cttcaaggcg
     4381 ctgggtgcgc cgattgcgct catacgcctg attcttcggc tgtatcctga tcgcggcatc
     4441 g
//
LOCUS       DATCXU010000225         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_576723,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000225 DATCXU010000000
VERSION     DATCXU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576723"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1026)
                     /locus_tag="VIR04_05055"
     CDS             complement(10..1026)
                     /locus_tag="VIR04_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460147.1"
                     /translation="MFEFSLSRKSLNHRIAALVRAAVATAAAIVFAVPCMAAGWPDRP
                     IQMVVPFPPGSSPDILARIISEPLAQALGQPVIVENKPGAGGNIGTRNASKAKPDGYT
                     ILLTINGPIVTAPALYKKTLGYDPNHDLKPISLVGTSPNILIVPASSPARTVKEFVAM
                     AKLKPGDLNYGTVGPGSSSHLSMAMLEHLAGISLQQIPYSSFPQIITSIINGDVQAGF
                     MVPGIAMPQITAQKVRALAITSLKPSDVLPGIPTMDSEGYAGFESISWDALFAPRDTP
                     DQIVQRFNMEITKILARPDVKQKMAALYFTPAPSSPGELTTLIHAETIRWGEVINRLN
                     LSLD"
     gene            complement(1060..2124)
                     /locus_tag="VIR04_05060"
     CDS             complement(1060..2124)
                     /locus_tag="VIR04_05060"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY9460148.1"
                     /translation="MTEPPLTRPRVTVLGAGSWGTALAAVACANAATLLWARDAGIAR
                     LVHEQHRNPRYLPDVALPRKLGCTSDFDLAIDHVCEPKTSGGLLILGVPVAGLAAVCE
                     KLADSLSRRRPKPLTVVWTCKGLHPETGLLPHQIARAALASQPQLGVGVLSGPSFAYE
                     VARGLPVALTVASGQPFVMHAVKLALHGGNVRIYTSEDVIGVEVGGALKNVIAIACGI
                     ADGLRLGANARAALITRGLAEIQRLGLALGGQADTFSGLTGLGDLVLTATGALSRNRQ
                     VGLALGQGQTLERILAGGMTAEGVRCASSALALGRKHSIDLPITQAVCDVLFNGMAPQ
                     HAVAGLLAREAKAESAISPE"
     gene            complement(2135..2653)
                     /gene="secB"
                     /locus_tag="VIR04_05065"
     CDS             complement(2135..2653)
                     /gene="secB"
                     /locus_tag="VIR04_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY9460149.1"
                     /translation="MADQDSTQNNQSESHDPSFSLQRTYIKDLSLEMPHAPAIFLEQE
                     GPTVEVAINIEGQRLAETIYECSVTATVTTRIGDKVLYLVEATQAGIFEAANIPAEQL
                     DPLLGIVCPTMLYPYLRANVADAINRTSLPALHLAEVNFQHLYEQRVAEAAKEKGEKD
                     SGLILPAGVTRQ"
     gene            complement(2699..2956)
                     /gene="grxC"
                     /locus_tag="VIR04_05070"
     CDS             complement(2699..2956)
                     /gene="grxC"
                     /locus_tag="VIR04_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY9460150.1"
                     /translation="MTPVTMYCTAVCPYCIRAEMLLKQRGVTEIQKIRIDRDPDQRAV
                     MMKRTGRRTVPQIYIGEAHIGGYDDLAALDRKNGLIPLLAN"
     gene            complement(2990..3403)
                     /locus_tag="VIR04_05075"
     CDS             complement(2990..3403)
                     /locus_tag="VIR04_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY9460151.1"
                     /translation="MDFILSQNNLYILVIALTSGAMLLWPTLMKGRNDTSVDIHQAVQ
                     LANQKQAVFLDVRTAEQFKAGSIPQARNLPAADIEAKLNTVPKNKPVIVVCNQGRDSA
                     KVVTSLRKHGFAEAVSLEGGLSNWSKEGMPLSKKA"
     gene            3493..4245
                     /gene="gpmA"
                     /locus_tag="VIR04_05080"
     CDS             3493..4245
                     /gene="gpmA"
                     /locus_tag="VIR04_05080"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY9460152.1"
                     /translation="MHKLVLMRHGESQWNLENRFTGWTDVDLTETGRMQARRAGEMLK
                     EQGFEFDLAYCSVLKRAIRTLWIALDAMEAMYTPIGMSWRLNERHYGALQGLNKSETA
                     AKYGEEQVLIWRRAYAVAPNPVAFDDPRHPRFDRRYAKVGADKLPTTECLKDTVQRVL
                     PFWNDSIAPALRAGRRVMVSAHGNSLRALVKHLDAISDSDIVALNIPTGQPLVYELDD
                     ELRPIRHYYLGDPAEIQAAMAAVAAQGKAKKD"
     gene            4247..>5073
                     /locus_tag="VIR04_05085"
     CDS             4247..>5073
                     /locus_tag="VIR04_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY9460153.1"
                     /translation="MRGALCVLVFGLWASTALAANSTSSLTARQAEAKKQQTELRARI
                     ETLQKEIDRQESSRRDAANDLKASESAISSINRRLADLAAQKRVAEHDLAGISDQITV
                     QKQQMAQRQRELADQLRAQYASGLSPWTALLSGDDPQAIGRDLSYLGYISQAQAESVR
                     AIRAAIKELAALQARSEARRKELGELAFETTEQKKALEQQQRDREKVLVRIESLLQKQ
                     RAQAAHMQRNDEHLGELISGLEKAIARQAELARQAEEKRKAEAARKAEQARKAALEQR
                     "
BASE COUNT         1034 a         1400 c         1612 g         1027 t
ORIGIN      
        1 ggcggggcat caatccagag acagattcag ccgattgatg acctcgcccc agcggattgt
       61 ctcggcgtgt attaacgtgg tgagttcgcc gggtgaggat ggcgcggggg tgaagtaaag
      121 cgccgccatt ttttgtttta cgtcgggccg tgccagaatc ttcgtgattt ccatattgaa
      181 tcgctgcacg atctgatcgg gggtgtctcg cggcgcgaac agcgcatccc acgagatcga
      241 ttcaaagccc gcgtagcctt ccgaatccat tgtcgggatg ccgggcagca catcgctggg
      301 cttaaggctg gtgatggcca gtgcgcgcac tttttgagcg gtgatttgcg gcatggcgat
      361 gccgggcacc atgaaacccg cctgtacgtc gccattgata atggacgtaa ttatttgcgg
      421 aaagctcgag taagggattt gttgcaggct aatgcctgca aggtgttcta gcatggccat
      481 gctcaggtgg gaagagcttc ctggcccgac cgttccgtag ttaaggtcgc ccggcttcag
      541 tttggccatt gccacaaatt ctttcactgt ccgcgctggc gaactggccg gcacgatcag
      601 aatgttgggg ctggttccaa ccagagaaat gggtttcagg tcgtggttcg ggtcgtatcc
      661 cagcgttttt ttgtaaagag ccggcgctgt gacgatgggg ccgttgatcg tcagcaaaat
      721 ggtgtacccg tcgggcttgg ctttgctggc gttgcgcgta ccaatgttgc cgcctgcgcc
      781 gggtttgttt tcgacgatga caggttgacc aagggcctgg gccagaggct cgctgatgat
      841 gcgcgcaaga atgtccggag aagagccggg cggaaaggga accaccattt gaatgggccg
      901 gtcgggccag ccggcggcca tgcaagggac ggcaaacaca atggcggccg cagtggcgac
      961 ggccgcacgg acaagggcgg cgatacggtg gttcagactt ttgcgactca gcgaaaactc
     1021 aaacatggaa attccttaag gcccggaagg gcatggtggc tattccggtg aaatggccga
     1081 ttctgctttg gcttcgcgcg ccagcagacc ggcaaccgcg tgttgcgggg ccatgccgtt
     1141 aaacagcacg tcgcaaaccg cttgtgtaat aggcagatca atgctgtgtt tgcggcccag
     1201 cgccagcgcc gaactggcgc agcgcacccc ttcggccgtc atgccgcctg ccagaatgcg
     1261 ctccagcgtc tggccttgtc ccaatgccag tccaacctgg cggttgcgtg aaagcgcgcc
     1321 ggttgccgtg agcaccaaat cgccgaggcc ggtcagaccc gaaaacgtat cggcctggcc
     1381 gccaagggca agcccaaggc gttgaatctc ggccagaccg cgcgtgatca gtgccgcgcg
     1441 cgcgttggcc cccaggcgca atccgtcggc aatgccgcag gcgatggcga tcacgttttt
     1501 cagggcgccg cccacttcca cgccgatgac gtcctcgctg gtgtagatgc gcacgtttcc
     1561 gccatgcagc gccaatttga cggcgtgcat gacgaagggt tgcccgctgg cgacggtcag
     1621 tgccaccggc aggccgcgcg caacctcgta ggcgaacgaa gggcccgaca gcacccccac
     1681 accaagctgc ggctgcgaag ccagcgctgc gcgtgcaatc tgatgcggaa gcagcccggt
     1741 ttccgggtgc aaacccttgc aggtccagac gacggttaat ggctttggcc ggcgccggga
     1801 aagggaatcc gccagttttt cgcagactgc ggccagaccc gccacaggaa cgcccaaaat
     1861 aagcagacca ccgcttgttt tcggttcgca cacatggtcg atagccagat cgaagtcgct
     1921 ggtgcaaccc agtttacgcg gcaacgcgac gtcgggcagg taacgcgggt tgcggtgctg
     1981 ctcgtgaaca aggcgggcaa tgccggcatc gcgcgcccac aataaagtag cggcattggc
     2041 acaggcaacc gccgccaggg cggtgcccca gctcccggcg cccagcaccg tgacgcgcgg
     2101 acgagtaagg ggcggctcgg tcatgggcgc actgctactg gcgcgtaacg cccgccggca
     2161 atatcaggcc cgagtctttt tcgccctttt ctttggccgc ttccgccacg cgctgctcgt
     2221 acaaatgctg gaagttcact tccgccagat gcagcgccgg aagcgatgtg cggttgatgg
     2281 cgtcggccac gttcgcgcgc aggtaagggt aaagcatcgt cggacataca atgcccagca
     2341 atggatcgag ctgctccgcg gggatgttgg ccgcttcgaa gatgccggct tgcgtggctt
     2401 cgaccagata aagcaccttg tcgccgattc gcgtggtgac ggtggccgtg acgctgcatt
     2461 cgtaaatggt ttcggccagg cgttgcccct cgatattgat cgccacttcg acggtcggac
     2521 cttcctgttc gaggaaaatc gccggcgcgt ggggcatttc cagcgacagg tctttgatgt
     2581 aggtgcgttg cagactgaag ctggggtcgt gactttctga ctgattgttt tgtgtcgaat
     2641 cctggtcggc catatggttt ccttaaaagg tgttggcagg gtaagtgtgg tcgtaaggtc
     2701 agtttgcaag caaagggatc aaaccgttct tgcggtccag cgcggccaga tcgtcgtatc
     2761 cgccgatgtg ggcctcgcca atatatattt gtggtaccgt gcgacggcca gtgcgtttca
     2821 tcatgacggc gcgttggtcc gggtcgcggt cgatgcgaat tttctgaatt tcggttacgc
     2881 cgcgttgctt gagcagcatt tcagcgcgta tgcaataggg gcacacggca gtgcaataca
     2941 tggttacagg ggtcatggtc actcgagtat gggcagtaaa aacaaagcgt tacgcttttt
     3001 tggacaaggg catgccttcc tttgaccagt tcgacaagcc gccttccaga ctaaccgctt
     3061 cggcgaaacc gtgcttgcgc aacgacgtca caaccttagc cgagtcgcgg ccttgattgc
     3121 aaaccacgat gacgggtttg tttttgggca cggtgttcag tttcgcctcg atgtcggcgg
     3181 ccggaaggtt gcgcgcctgc gggatgctgc ctgccttgaa ttgctcggcg gtgcgaacat
     3241 ccaggaaaac ggcttgtttc tgattggcca gttgcacggc ctggtgtatg tcgaccgatg
     3301 tgtcgttgcg tcctttcatt aaggtgggcc atagaagcat cgcacccgat gtaagggcga
     3361 taaccagaat atagaggttg ttttgactga gaataaaatc cacgttgtat cccgaattcg
     3421 cccccaaaat gagggctcaa taagacaatt ataaaatgga cagaataatc caattttcag
     3481 gcgattttta ttatgcataa acttgttctc atgcgccacg gcgagagtca gtggaacctc
     3541 gaaaatcggt tcaccggatg gaccgatgtc gacttgaccg aaacgggccg catgcaagcc
     3601 cggcgcgccg gcgagatgct taaagaacag gggtttgagt tcgacctggc ctattgttca
     3661 gtgttgaaac gcgcgatccg cacgctgtgg attgcattgg acgccatgga ggcgatgtac
     3721 acgcccatag gcatgagctg gcgtttgaac gagcgccatt atggcgcgtt gcaaggcctg
     3781 aacaaatctg aaacggcggc aaaatacggc gaggaacagg tgctgatatg gcgtagggca
     3841 tacgctgttg cgcccaatcc cgtcgccttt gatgatcctc gtcatccgcg gtttgatcgc
     3901 cgctacgcca aggtgggcgc cgataaactt cccaccacgg aatgcctgaa ggatacggtg
     3961 caacgggtgc ttcccttctg gaatgattcc attgcgccgg ccttgcgcgc ggggcggcgc
     4021 gtcatggtgt ccgcgcatgg caacagcttg cgcgcgctgg tcaaacatct cgatgcaata
     4081 tccgacagcg atatcgtcgc gctgaatatt cccaccgggc agcccttggt gtacgagctg
     4141 gatgacgaat tgcggcccat tcggcattat tatctgggcg atcccgctga aatccaggcg
     4201 gcgatggcgg cggtggcggc gcagggtaaa gctaaaaagg actagcatgc gcggtgcgtt
     4261 gtgtgttttg gtatttgggt tatgggcaag caccgcgctg gccgccaatt ctacctcctc
     4321 gctgaccgcg cggcaggcag aagcgaaaaa gcagcagacc gaactgcgcg cacgcatcga
     4381 aactctgcaa aaagagattg accggcagga gtcgtcgcgg cgcgacgcgg ccaacgattt
     4441 gaaggcgtcc gaatcggcca tttcgtccat taaccggcgc cttgccgacc tggccgctca
     4501 aaaacgggtt gccgagcatg atctggccgg gatcagcgac cagataacgg tgcagaagca
     4561 gcaaatggcg cagcgccagc gcgagctggc tgaccagttg cgtgcgcaat atgccagtgg
     4621 gctgtctccg tggacggcct tgttgtcggg cgacgatccc caggccatag gccgcgatct
     4681 gagctacctg ggctatatct cccaggccca ggcggagagt gtgcgcgcca tccgcgccgc
     4741 gattaaagaa ctggccgcat tacaggccag atccgaggcg cgccgcaagg aactgggcga
     4801 gctggcgttc gagacaacgg agcaaaagaa ggcacttgag cagcagcaac gggatcgcga
     4861 aaaggttttg gtccggatcg agtccttgtt gcagaagcag cgggcgcagg cggcgcacat
     4921 gcagcgcaat gacgaacatt tgggcgagtt gatcagcggg ctggagaagg ccattgcgcg
     4981 gcaggcggaa ctggctcgtc aggcggaaga aaaacgcaaa gccgaggccg cccgcaaggc
     5041 cgagcaggcg cgcaaggcgg ctttggagca gcg
//
LOCUS       DATCXU010000226         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_720795,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000226 DATCXU010000000
VERSION     DATCXU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720795"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2207)
                     /locus_tag="VIR04_05090"
     CDS             complement(<1..>2207)
                     /locus_tag="VIR04_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY9460154.1"
                     /translation="EPQKPEQIVAQLLRDYKGRHVGILAPMITARKGYYTELAKWAAG
                     KGYTHLRVDGAFTPVAPWPRLDRYKEHTLELPIADLIVDPANEPALRDAVRSALAHGQ
                     GSLSVLLDLTGDSTAGLAERRAEGLAETTEQRHFSVKRACPNCGTSFPEPDPRLFSYN
                     SPHGWCPGCFGTGLQLSGFDEEQTGEETIWNAWYGDEPQVCASCHGERLNPVALAFRW
                     RERSIAELAALPISDAQTFFSGLIAHGREAEIARDILAEIRARLNFMQAVGLGYLALD
                     RAAPTLSGGEAQRIRLAAQLGSNLQGVCYILDEPTIGLHPRDNQILLDALAKLENNGN
                     SLVVVEHDEDTIRRASHLIDIGPGAGIRGGRVVAQGTVQDIIDCPASVTGQYLKHPLQ
                     HPQQERRAVTPETPAIEIISAHMHNLHNVNARFPVGRLSVVTGVSGSGKSTLAREVLL
                     DNMAQAISQRKAVAWHGCADITGWEKIDRVLEVDQTPIGKTPRSCPATYIGFWNDVRQ
                     LFAQTREARIRGWSTARFSFNTGEGRCPICEGQGLRTVEMSFLPDVKVPCDACNGERF
                     NRDTLNVTWRGKNAGEVLKLEVDEAIDYFAAHSKILRPLQLMHDVGLGYLTLGQPSPT
                     LSGGEAQRIKLVTELAKARLTDGLIKTGRASRVPHTLYILDEPTVGLSMADVEKLIHV
                     LHRLVDAGNTVVVIEHNLDVIAEADWVLDLGPEGGTGGGRMVAQGTPEQIIAL"
BASE COUNT          420 a          632 c          672 g          483 t
ORIGIN      
        1 ctcaaggcaa taatttgctc gggcgtacct tgggcgacca tgcgtccgcc gccagttccg
       61 ccttcgggtc ccaggtcaag cacccagtcg gcttcggcaa tcacatcgag gttatgttcg
      121 ataaccacca cggtgttgcc agcgtcgacc agtcgatgca ggacgtgaat aagcttttcg
      181 acgtccgcca tcgataatcc gacggtgggt tcgtcaagga tatacagcgt atggggtacg
      241 cgcgatgccc ggccggtttt tataaggccg tcggtgagtc tggctttggc cagttcggta
      301 acgagcttga tgcgttgggc ctcgccgccg gacaaagttg gcgatggctg tcccagtgta
      361 aggtagccaa gtcccacgtc gtgcatcaat tgcaggggcc gtaaaatttt tgaatgtgcg
      421 gcaaagtagt cgatggcttc atccacttca agcttgagca cttcgcccgc gtttttgccg
      481 cgccacgtca cattcagcgt atcgcgattg aaacgctcgc cattgcaggc gtcgcaaggc
      541 accttgacat ccggcaggaa actcatttcc acagtgcgca agccttgtcc ttcgcagatg
      601 gggcagcgtc cctcacctgt gttgaatgaa aatcgggctg tgctccagcc gcggatgcgc
      661 gcctcacgcg tctgggcaaa caattgccgg acgtcgttcc agaatccgat atacgtggcc
      721 gggcacgacc gcggcgtttt tccgatggga gtctgatcaa cctccagcac tcggtctatc
      781 ttttcccagc ctgtaatatc ggcgcagcca tgccatgcga cggctttgcg ctgcgagatg
      841 gcctgagcca tgttgtcgag caatacttcg cgggcaagcg tggatttgcc cgaacccgac
      901 acgccggtga cgacgcttag ccgcccgacc gggaagcgcg cattgacgtt gtgcaagtta
      961 tgcatgtgag cgctgatgat ttcaatggcg ggcgtttccg gtgtgacggc gcggcgctct
     1021 tgttgcgggt gttgcagcgg atgttttaag tactgacccg tgaccgacgc ggggcagtcg
     1081 ataatatctt gcaccgttcc ttgcgccacg actcgcccgc cgcgtatccc ggctccaggc
     1141 ccgatgtcga taaggtgcga agcgcggcgt attgtgtcct cgtcgtgttc gacgaccacc
     1201 agcgaattgc cgttattttc gagtttggcc agcgcgtcga gcagaatttg attatcgcgc
     1261 gggtgcaggc cgatggttgg ttcgtccaga atgtagcaga ccccctgaag atttgaaccc
     1321 agttgcgcgg ccagccgaat gcgctgcgct tcaccgccgg atagagtcgg ggcggcgcga
     1381 tccaaagcca gataacccag gcccacagcc tgcataaaat tcagtcgtgc gcggatttcc
     1441 gcaaggatat cgcgagcgat ttcggcctcg cgtccatgcg cgatcagacc tgagaagaag
     1501 gtttgggcat ccgatattgg cagcgcggcc agttcggcaa tcgagcgctc gcgccagcga
     1561 aacgcgagcg ccaccggatt gagccgttct ccgtgacaac tggcgcagac ttgcggctcg
     1621 tcgccatacc atgcattcca gatggtttct tcgccggttt gttcctcgtc aaagccgcta
     1681 agttgcaggc ccgttccgaa gcaaccgggg caccagccat gcggggagtt atacgaaaat
     1741 agccggggat cgggttcggg gaagctggtt ccgcaattgg ggcaggcgcg tttgactgaa
     1801 aagtggcgtt gctctgttgt ttcggccagg ccttccgcgc ggcgctcggc cagacctgcg
     1861 gtggaatcgc cggtcagatc aagcaagacg ctcaacgatc cctggccatg tgccaaagcg
     1921 ctgcgcacgg cgtcgcgtaa ggcaggttca ttggcggggt cgacaatcag gtcggcaata
     1981 ggcagctcga gcgtatgttc tttataacga tccaggcgcg gccacggagc aacaggggtg
     2041 aaggcgccgt cgacgcgcaa atgcgtatag cctttgcccg ctgcccattt ggccagttcg
     2101 gtgtagtagc ctttgcgcgc ggtaatcatg ggagcaagaa tgcccacatg cctgccttta
     2161 tagtcgcgta acagttgcgc gactatttgc tctggctttt gcggctc
//
LOCUS       DATCXU010000227         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_857808,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000227 DATCXU010000000
VERSION     DATCXU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857808"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VIR04_05095"
     CDS             complement(<1..232)
                     /locus_tag="VIR04_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY9460155.1"
                     /translation="MTFSPPASIDSFLDAVWLEDGLAENTLAAYRRDLTAFARWLQTR
                     PNMDLDKVQTLDIQEWFVARHAASKATTANRRL"
     gene            416..1177
                     /gene="modA"
                     /locus_tag="VIR04_05100"
     CDS             416..1177
                     /gene="modA"
                     /locus_tag="VIR04_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9460156.1"
                     /translation="MMTNRHLVAIAAMLCILAWSAQTVAADVVVSAAASLTNAFKAMA
                     DQFQAGHRTIKIHMNFGASDVVAQQIINGAPVDVFASADEKAMDKAVAANVIVPSTRI
                     NFAGNEVVLIVPAASRLEIGSVQDLARAHVKSVTFGNPASVPVGRYTKAALESVHLWD
                     SLQTKAVEAQNVRQALEYVARDEVDAGFVFSTDAAIMPARVRVVEHLVSPAPVLYPIA
                     LTRREHRQPEAQLFVDFVLSPAGQAILTRFGFKRP"
     gene            1230..1916
                     /gene="modB"
                     /locus_tag="VIR04_05105"
     CDS             1230..1916
                     /gene="modB"
                     /locus_tag="VIR04_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY9460157.1"
                     /translation="MTGLTVPLLLSLKVAAWATVFATLLGVAAAFGLTRWRSSWCDFV
                     DSVLTLPLVLPPTVMGYYLLVLVGRRGWLGALLEKWGIQLVFTWQGAVLAATVVAFPL
                     VLKAARAAFNDVDPKFENAARVLGVGESALFFRVTLPLALRGIFAGVLLAFARALGEF
                     GATLMIAGSIPGRTQTLSIAIYEAVQAGDDSLANTLVLVTSVTCIAVLWIASRLLPVY
                     SRGSRNRGRS"
     gene            1913..>2346
                     /locus_tag="VIR04_05110"
     CDS             1913..>2346
                     /locus_tag="VIR04_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460158.1"
                     /translation="MTVSVDIRRTIVSGEREFVLNVAFESDKRRLALFGPSGAGKTLT
                     VRAIAGLMQPDSGHIRIKDRVFFDSEKHINLAPQARRAAYLFQEYGLFPHLTVAQNIC
                     FGLTKRWRNPRRNDLPDAAKKWVSAFELKSLIHSYPSEISGG"
BASE COUNT          437 a          604 c          728 g          577 t
ORIGIN      
        1 ccagccggcg gttggccgtc gttgcctttg aggcggcgtg acgcgcgaca aaccactcct
       61 ggatatccag ggtttgcacc ttgtccagat ccatgttcgg tcgagtctga agccaccgcg
      121 cgaatgccgt aagatcgcgg cgataggcgg ccaacgtgtt ttcggccagc ccgtcttcga
      181 gccagacggc atccaggaat gaatcgattg aggcgggtgg agagaaagtc aaagcaggtg
      241 tcgaaaaaaa ggcggtgtca agcgtttcat cgaatatttc ttccgattct acagtgtgcc
      301 gccagtgccg gctatttttg gctttggcgg gttgactgtc gttatatacc tatatatatt
      361 aaaaactcac atcccagcga acatcaggat cgatgatgac gaccgtggcc gaatcatgat
      421 gacaaacagg catcttgtcg cgatagccgc gatgctgtgc atattggctt ggtcggcgca
      481 aacggtcgcg gctgatgtcg tcgtatcggc ggcggccagc ctgactaacg ccttcaaggc
      541 gatggcggac caatttcaag ccggccaccg cactattaaa atccacatga attttggcgc
      601 gtcggacgtt gtggcgcaac agattatcaa tggcgcgcca gttgatgtat ttgcgtcggc
      661 cgatgaaaag gcaatggata aggcggttgc cgcaaacgtg atcgtccctt caacgcgaat
      721 caatttcgcg ggaaacgagg tcgtcctgat tgtgcccgcc gccagcaggc ttgaaatcgg
      781 atccgttcag gatttagccc gcgcgcatgt caaaagtgtg acgtttggca acccggcgtc
      841 ggtccctgtg ggccgttaca ccaaagcggc gctggagagt gtgcacttgt gggattctct
      901 gcagactaaa gcggtagaag cccaaaatgt acgtcaggcg cttgagtatg tggctcgcga
      961 cgaggtcgac gccgggtttg tattttccac tgacgcggcg attatgccgg cccgggtcag
     1021 ggtcgtcgaa catttggtct cgccggcgcc cgtgttgtat ccgattgcac tgacgcgtcg
     1081 cgaacaccgt cagccagaag cgcaattgtt tgtggatttt gtgctttcgc cggcggggca
     1141 ggccatcctg acccggttcg gattcaagcg gccatgacca aggcgtcctg cttgtggtgt
     1201 gcggggccgc cagccgcagc gtggtgcgta tgactggact gacggttcct ttgttgctgt
     1261 cgctcaaggt ggcggcatgg gcgaccgtgt tcgcgacttt attgggggtt gccgccgcct
     1321 ttggcttgac gcgctggcgt tcctcctggt gcgacttcgt cgattcggtg ttgacattgc
     1381 cgctggtttt gccgcccaca gtcatggggt attacctgct ggttttggtg ggtcgccgcg
     1441 gctggttggg tgccttgctg gaaaaatggg gaattcaatt agtttttacg tggcagggcg
     1501 ctgttctggc cgccactgtg gttgcctttc cgcttgtttt aaaggcggcg cgggccgcct
     1561 ttaacgacgt tgaccccaag ttcgagaacg cggcccgggt tctgggagtc ggcgaatctg
     1621 cgcttttttt ccgagtgacc cttccgttgg ctttacgcgg aatttttgcg ggtgtgttgc
     1681 tggcgttcgc gcgcgcgctg ggcgaatttg gcgcgacctt gatgattgcc ggcagcattc
     1741 cgggccggac gcaaaccttg tctattgcca tttacgaggc ggtgcaggcc ggcgacgata
     1801 gtctggccaa cacgcttgtg ctggttacgt ccgtgacctg cattgcagtg ctctggatcg
     1861 ccagtcgttt gttgccagtt tattcgcgcg gatcacgcaa tcgagggcgc tcatgaccgt
     1921 aagcgttgac atcaggcgca ccattgtttc gggcgagcgg gagttcgttt tgaacgtggc
     1981 tttcgaatcg gacaaacgcc ggctcgcgct gtttgggccc tcaggagcgg gtaaaacact
     2041 gacggttcgg gcaattgcgg gtttgatgca acccgacagc gggcatatca ggatcaaaga
     2101 cagggttttt tttgattccg aaaagcacat taatctggcg ccgcaggcgc gtcgcgccgc
     2161 ctatttgttt caggaatatg ggttgtttcc tcacttgacc gttgcgcaaa acatttgttt
     2221 tggtttgacg aaacgctgga gaaatccgcg gcgcaatgac ttgcccgatg ccgccaaaaa
     2281 atgggtttcg gcgtttgagc tgaaatcgct gatacacagt tatccctcgg aaatatcggg
     2341 tggaca
//
LOCUS       DATCXU010000228         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1124360,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000228 DATCXU010000000
VERSION     DATCXU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124360"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VIR04_05115"
     CDS             complement(<1..187)
                     /locus_tag="VIR04_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460159.1"
                     /translation="MNRPHGHTGPKKNRQMLRVCVAAVSLALLSACAAPPHTAAGVQD
                     AAELTPATPSTRDLLQLP"
     gene            complement(184..633)
                     /locus_tag="VIR04_05120"
     CDS             complement(184..633)
                     /locus_tag="VIR04_05120"
                     /inference="COORDINATES: protein motif:HMM:NF022082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HEY9460160.1"
                     /translation="MKEETRPTTQIRQAFAALLMSAVCGGATASQIIYTPVNPAFGGN
                     PFNGAVLLDKAQAQNKYTDPDLGSSALDAQTTPLQDFNNTLQRSILSRVAATASSQII
                     GNDGTLHPGTVETSDFTISVVDVGGGLLKITTTDKASGAQTSFQVGQ"
     gene            complement(634..1086)
                     /locus_tag="VIR04_05125"
     CDS             complement(634..1086)
                     /locus_tag="VIR04_05125"
                     /inference="COORDINATES: protein motif:HMM:NF022094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HEY9460161.1"
                     /translation="MSLQNRRSIVVVAFLLATPLAHAGSAPAPNPVPDESRPASEQDL
                     LGGVITSRTITVLGWDFYEYFTAKWQAIHGNERLSLSIYERPTARWGSEIWIDYRQTR
                     VFHTFLPPARSATKEISTKAVDFVYKNVTDINIQRSLFTDVDLAPEEM"
     gene            1379..1455
                     /locus_tag="VIR04_05130"
     tRNA            1379..1455
                     /locus_tag="VIR04_05130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1413..1415,aa:Pro,seq:ggg)
     gene            1593..2654
                     /locus_tag="VIR04_05135"
     CDS             1593..2654
                     /locus_tag="VIR04_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY9460162.1"
                     /translation="MTTLLDPVQLGAVFLKNRVVMAPLTRARATVERTPNELMRHYYC
                     QRADAGLILTEATAVSPGGVGYPNTPGIWSHAQIAGWQKITRSVHDKGGKIFLQLWHA
                     GRVSDPEYLNGEIPVAPSAIRCEGKVSLVRPERDYVVPRALHLEEISGVVGDFAQAAR
                     NAKEAGFDGVEVHGANGYLIDQFLQDGSNKRGDQYGGPIPNRARLLFDIVDACIGVWG
                     ADRIGVHLSPCGTLHSMFDSDPAALFNYVARQLGQRKIAFVFVREAPDGASRSAVIKR
                     MFNGHMIINDRHSIASAQRALIDGCADAIAFGKAFIANPDLVTRLRMGADLNDCDPGT
                     FYSGGPKGYTDYPTLRADA"
     gene            2747..3169
                     /locus_tag="VIR04_05140"
     CDS             2747..3169
                     /locus_tag="VIR04_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460163.1"
                     /translation="MSLFTTKEVRVGSVCLSVAAMLSGCALGIHPGDNSPSVSYTVPR
                     SFQMVFLRAQNQAQECLRGKGEYKVHARVDPATRSGIVSVQGPLANEVARTDFKATDE
                     KHTQVTQTVWGHSPWDANALDAMRESVRMDTSICFAYK"
     gene            complement(3181..>3367)
                     /locus_tag="VIR04_05145"
     CDS             complement(3181..>3367)
                     /locus_tag="VIR04_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY9460164.1"
                     /translation="LPQPATVQEVEAAIQAAIAQVTNQGITGAPAMGKVMALLKTTLA
                     GRAEMSAVSRQVKAQLM"
BASE COUNT          680 a          845 c         1003 g          839 t
ORIGIN      
        1 gcggcaattg cagcaggtcg cgcgttgaag gggttgcagg ggtcaattcg gcggcgtctt
       61 gtacgccagc ggccgtatgg ggcggcgcgg cgcacgcgct tagtaatgcc agactgacgg
      121 cggcaacaca gacgcgcagc atctggcgat ttttcttggg gcctgtatgg ccgtgcgggc
      181 ggttcattgt cccacctgaa atgaagtttg ggcgccgctt gccttgtcgg ttgtggtgat
      241 tttcagcagg ccgccgccta cgtcaacgac gctgatcgtg aaatcacttg tctccacagt
      301 tccgggatgc aacgtgccat cgttgcctat gatttgagac gatgccgtcg ccgccacgcg
      361 gctcaatatg gaccgctgca acgtgttatt gaagtcctgc agaggcgttg tctgggcgtc
      421 gagcgcgctg gatcccagat ccgggtcggt gtatttgttt tgcgcttgcg ccttgtccag
      481 cagcaccgcc ccattgaaag ggttgccccc gaacgcggga ttcacaggcg tatatattat
      541 ttgcgatgca gtggcgccac cgcaaacggc cgacatcaga agcgctgcaa aggcctgcct
      601 gatttgagtt gttggtctgg tttcttcttt catttacatc tcctcgggcg cgagatccac
      661 atcggtgaac aacgaacgtt ggatgttgat gtcggttaca tttttataaa caaagtccac
      721 ggctttcgtg cttatctctt tagtggccga gcgagcgggc ggcaaaaatg tatgaaacac
      781 tcgcgtttgc ctgtaatcga tccagatttc actgccccat cgggctgtcg ggcgttcata
      841 gatggacagc gaaagacgct cgttgccatg gatggcctgc catttggctg taaaatattc
      901 gtaaaagtcc catccgagta cggtgatagt gcgactggtg atcacgccac ccagcagatc
      961 ctgttcggaa gcggggcggc tttcatcagg aaccggattg ggtgccggcg ccgaccccgc
     1021 gtgtgcaagc ggcgtagcaa gcaagaaggc aacaacgacg atcgaacgtc tgttctgcaa
     1081 actcatttgg ctccctcttt gttgcaactc gtatcaacgg ctggtttttt gtttacgact
     1141 gccacgttct cttaacgtct ggaaacaaaa tcaatgcttg tcatcataaa tagacgcaat
     1201 cggggacggt atgggccgta tgaagtaggt tcgtacgccc taaaaggatt aggatgttga
     1261 tgtgaaatca ctgagctttc ctcgcccggg cgggcagtgg aatttgtgct agaatcgcct
     1321 tcgtcgcggg cttgtattgg ctgggtaacc ggccagcctg agcggctttg gctgaattcg
     1381 gggcgtagcg cagcctggta gcgcacttgc atggggtgca aggggtcgaa ggttcaaatc
     1441 cttccgttcc gaccaattaa ataaaaggct tgcgtggtga accacgcaag ccttttgtca
     1501 tttttgaatt tcaggatggc aggcttgttt ctgcatttaa ttcgaatatg gtgcaaacta
     1561 gggtaaacct gaattgatgc cttattttta ccatgacgac tttgcttgat ccggttcagt
     1621 taggagccgt atttttgaaa aaccgcgttg taatggcccc tttgacgcga gcccgtgcaa
     1681 cggtggaacg cactcccaac gaattgatgc ggcattatta ttgccagcgc gccgatgcgg
     1741 gtcttatttt gaccgaggct acagcggtgt cgcctggagg ggtggggtat ccgaacactc
     1801 cgggtatttg gtcccatgcg caaattgccg gctggcagaa aatcactcgc tccgtacacg
     1861 ataaaggtgg caaaattttt ttgcagttat ggcatgcggg gcgcgtttcg gatccggaat
     1921 atctcaatgg cgagattccg gttgcaccca gtgcgattcg ctgcgaggga aaggtcagtt
     1981 tagtgcggcc cgagcgcgat tatgttgtgc ctcgcgcatt gcatctggaa gaaatttcgg
     2041 gcgttgtcgg cgactttgcc caagccgcca gaaatgccaa agaggccggc ttcgatggcg
     2101 tggaagtgca tggcgccaac ggatatttga tcgatcaatt tttgcaggat gggtcaaaca
     2161 agcgcggcga tcagtatggc ggcccaattc ccaatcgggc gcgattgctg tttgatatcg
     2221 tcgacgcctg tatcggggtt tggggcgcgg atcgaatcgg agtccatttg tcgccttgcg
     2281 gcacactgca ctccatgttt gattcggacc ctgcggcctt gttcaattat gtggccaggc
     2341 aattgggtca acgcaaaatt gcatttgttt ttgtgcggga ggcgcctgat ggcgcgagcc
     2401 ggtccgccgt catcaagcgc atgtttaatg gacatatgat tatcaatgat cgccatagta
     2461 tcgccagcgc tcagcgggca ttgatcgatg gttgcgccga cgccattgcg tttgggaaag
     2521 cgtttatcgc caaccccgat cttgtgactc gcttgcggat gggcgcggac ttgaacgatt
     2581 gcgacccggg tacgttctat agtggtggcc ccaaagggta taccgattac cccaccttga
     2641 gggccgatgc ctgaccggcc ggtcgggtcg aatgtgcaag aaaggctagc gcattatgct
     2701 ggattgcgtt acgctcgttt cgtttcagga tggtaaagga atcagggtga gtctttttac
     2761 aactaaagaa gttcgtgtcg ggagcgtatg tctaagcgtg gcggcgatgt tgtccggttg
     2821 cgcgttgggc atacatcccg gagacaactc ccccagcgtt tcttataccg tgccgcgcag
     2881 ttttcaaatg gtgtttttgc gtgctcagaa ccaggcgcag gaatgtttgc gcggcaaggg
     2941 cgaatacaag gtgcatgccc gcgtcgatcc cgcaacccgg tcgggcatag tgtcggtgca
     3001 gggcccgctt gcaaacgagg tcgcccgaac tgatttcaag gcgaccgacg aaaagcacac
     3061 ccaggtaacg cagactgtat gggggcatag cccctgggac gcgaatgcgc tggatgccat
     3121 gcgtgaatct gttcgtatgg atacgtcgat ctgtttcgcg tacaagtagc gcggcctgta
     3181 ttacatcaat tgcgctttga cctgcctgga gaccgccgac atttcagccc ggcctgcaag
     3241 tgttgtcttt aatagcgcca tgactttgcc catggctggc gcgcctgtaa tcccctgatt
     3301 ggttacttgg gcgatggcag cctggattgc cgcttcaact tcttgcacgg tcgcgggttg
     3361 cggcagg
//
LOCUS       DATCXU010000229         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1054605,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000229 DATCXU010000000
VERSION     DATCXU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054605"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VIR04_05150"
     CDS             <1..294
                     /locus_tag="VIR04_05150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517935.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY9460165.1"
                     /translation="KRERMKTGMEDLIHHFKLFTEGMHVPEGEAYAAIEHPKGEFGIY
                     LVSDGANKPYRLKIRASGFAHLQALDEMSRGHMIADVVTIIGTLDIVFGEIDR"
     gene            331..825
                     /gene="nuoE"
                     /locus_tag="VIR04_05155"
     CDS             331..825
                     /gene="nuoE"
                     /locus_tag="VIR04_05155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY9460166.1"
                     /translation="MLLSEQAYQKIDRELTKFPDDRKRSAILASLIIAQDEKHWISPD
                     VIEDVATYLGVPPIAVQEVASFYNMLNIKPTGRYKLTVCTNLPCALRNGEKAVEHLKQ
                     ALSVDLNEISADGLFTVIEGECMGACGDAPVVLVNNKQMCVRMSDEKLDAMIAELKQH
                     GEPA"
     gene            825..2189
                     /gene="nuoF"
                     /locus_tag="VIR04_05160"
     CDS             825..2189
                     /gene="nuoF"
                     /locus_tag="VIR04_05160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY9460167.1"
                     /translation="MVAPDLRQFSQGLEPNPGNDLSNSLLFHDRHITPQIVAGLNGSN
                     WGLDDYVKRGGYEALRKVLTSGMKREDVIAEVKASGLRGRGGAGFPTVLKWSFMPRNL
                     PVQKYLICNSDEGEPGTFKDRDILRFNPHAVIEGMAIGAFAMGIDVGYNYIHGEIYEV
                     YQRFEQALEQAYAAGFLGQKIMGSDFNFTLHAFSGYGAYICGEETAMLESLEGKKGLP
                     LFKPPFPAGFGLYGKPTTINNTETFAAVPWIFRHSAQEYLEIGLPNNGGTKLFSVSGD
                     VERPGNYEIPLGTPFVKLLELAGGVRNGHQLKAVIPGGSSAPVLPADAAMKLTMDYDS
                     IAKAGSMLGSGAVIVMDETRCMVKSLLRLSYFYFEESCGQCTPCREGTGWLFRMVNRV
                     EHGEGRPEDLEVLDSVAHNIMGRTICAFGDAAAMPIRSFIKHFRDEFAYHIQHKNCMV
                     DKYL"
     gene            2205..4532
                     /gene="nuoG"
                     /locus_tag="VIR04_05165"
     CDS             2205..4532
                     /gene="nuoG"
                     /locus_tag="VIR04_05165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY9460168.1"
                     /translation="MVELTIDGIKVEAPEGSMVIQAAHKLGVYVPHFCYHEKLTIAAN
                     CRMCLVDVEKVGKPLPACATPVTNGMIVHTHSQKAIDAQKSVMEFLLINHPLDCPVCD
                     QGGECQLQDLAVGYGASASRYKEEKRVVVHKEIGPLVSAQEMQRCIHCTRCIRFGIEI
                     GGLMELGMVNRGERSEITSFVGRAVESELSGNMIDVCPVGALTSAPFRFAARTWELAR
                     RRSVSPHDSLGANLVVQVKGERVMRVVPFDNEDVNECWISDRDRFAYEGLYTEDRLAH
                     PMIKGPDGDWREASWSDALQQVVKGLNNVRDQYGAEQIGALSSHYATTEEFALLARLT
                     RALGSENVDFRLRYMDPGFDQAVPGVPWLGLPIAELNQLDRVLVVGSFLRKDHPLMTQ
                     RLRQAAKRGTQITLIESAADDPLLPVAARLTVAPSLLPNALAEVLVAVARAKQQPVPA
                     ELAGVEPSDAANQIAASLTSGAKTAVLLGNMAVTSNQASLIAANAFALAKAVSARFGF
                     LTVGGNTVGGYLAGAIPRRGGLNAEQMLDQPLKAYLVMHAEPFLDADNGVRATEVLKS
                     ADFAVALTSYRSAAQDWADVMLPIAPFTETSGSFVNAEGRVQSFKGTVAPFVDTRPAW
                     KILRVLGNLFQLQGFDDETSESVRDSIMVGGIESRLSNDVVGPVGTSAKLDGLERVAD
                     VPIYRSDSMVRRSAPLQLPPVSQPPKARMTADTLASLGVQAGEMVRVRSAQGEILLAA
                     QHDDTVAPGCVRIAAAFAETAPLGGAFGQLTVERA"
     gene            4534..>4841
                     /locus_tag="VIR04_05170"
     CDS             4534..>4841
                     /locus_tag="VIR04_05170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY9460169.1"
                     /translation="MDWLKVVENYGTGLVGPTAWLVIWILVKIVVIAVPVILCVAYLT
                     YWERKLIGAMHVRLGPTKVGYKGLLQPFADVFKLLTKEVIVPTKANKVLYILAPVVTL
                     "
BASE COUNT          990 a         1324 c         1458 g         1069 t
ORIGIN      
        1 aaacgtgaac gcatgaaaac gggcatggaa gaccttatcc accatttcaa gctgttcacc
       61 gaaggaatgc acgtgcccga gggcgaggcc tacgccgcca tcgaacaccc gaagggcgaa
      121 ttcgggattt atctggtgtc cgacggcgcc aataagcctt accgccttaa aattcgcgcc
      181 tcgggttttg cgcatttgca ggcgcttgac gaaatgtcgc gtggacacat gatcgcggac
      241 gtcgtcacca ttatcggaac gctggacatt gtgtttggcg aaatagatcg ttgattggcg
      301 tcacaccgac gcggaaacca ggttcaaatt atgttgcttt ctgaacaagc ctaccaaaag
      361 attgaccggg agctgaccaa atttcccgat gacaggaagc gatcggccat tttggcgtcg
      421 ctgatcatcg cgcaagatga aaagcactgg atttcacccg acgtcattga ggacgtcgcg
      481 acgtatttgg gcgtgccgcc catagcggtt caagaggtcg cttcgtttta caacatgctc
      541 aatatcaagc cgaccggccg ttacaagctg accgtttgca ccaacttgcc ctgtgcgttg
      601 cgcaatggcg aaaaagccgt cgagcacttg aagcaggcgt taagcgttga cctgaacgaa
      661 atttccgccg acggcttgtt taccgtgatc gagggcgagt gcatgggcgc atgcggcgac
      721 gcacccgtgg tgctggtcaa caacaagcaa atgtgtgtgc gcatgtccga cgaaaagctc
      781 gacgcgatga ttgcggaact taaacagcac ggagaaccgg catgatcgta gcgcctgatt
      841 tgcggcagtt ttcccaaggt ctcgagccca accccggcaa cgatctctcc aatagcctgc
      901 tgtttcacga ccgccatatc acgccgcaaa tcgtcgcggg gctgaatggt tctaattggg
      961 gcctggacga ttacgtcaaa cgcggcggtt acgaggcatt gcgcaaggta ttgacctcgg
     1021 gcatgaaacg cgaagacgtc attgccgagg tcaaggcttc cgggttgcgt gggcgcggcg
     1081 gcgccggttt tccgaccgtc ctgaaatgga gctttatgcc gcgcaacctt ccggttcaga
     1141 aatacctgat ctgcaattcg gacgaaggcg agcccggcac attcaaagat cgcgatatct
     1201 tgcgttttaa tcctcatgcc gtgattgagg ggatggccat cggggccttc gccatgggca
     1261 tcgacgtggg ctataactat attcacggcg aaatctatga agtctatcag cgcttcgaac
     1321 aggcgctgga gcaggcgtat gcggcgggtt ttctgggcca gaaaatcatg ggctcggatt
     1381 ttaactttac gctgcacgct ttttcaggct acggcgccta catctgcggc gaagaaaccg
     1441 ccatgctgga atcccttgaa gggaaaaagg gtttgccgct tttcaagccg ccatttccgg
     1501 ccggcttcgg cttgtacggc aagccgacca ccatcaacaa tactgaaacg ttcgccgccg
     1561 tgccctggat cttccgccac agcgcgcagg aatatctgga aatcgggttg cccaacaatg
     1621 gtggcaccaa gctgttttcg gtctccggcg acgtcgaacg ccctggcaac tacgagatcc
     1681 cgctgggcac tccgtttgtg aagttgcttg aactggccgg cggtgttcgg aacggccacc
     1741 agttaaaagc agtaattcca ggcggctcca gtgcgccggt gttgccggcc gacgctgcga
     1801 tgaagctgac gatggattac gattccatcg ccaaagcggg ctccatgctg ggctcgggcg
     1861 cggtgatcgt catggatgaa acccgctgca tggtcaagtc gctgttgcgg ttgtcgtatt
     1921 tctatttcga ggaaagctgc ggccagtgca cgccgtgccg cgaaggaacg ggctggcttt
     1981 ttcgcatggt caaccgggtt gagcacggcg aggggcgtcc cgaagacctg gaagtccttg
     2041 attcggttgc gcacaacatc atggggcgta ctatttgtgc tttcggcgat gcggcagcca
     2101 tgccgatacg cagtttcatt aagcactttc gcgacgagtt cgcataccac attcagcata
     2161 aaaactgcat ggtggataaa tacttgtagg gtcaggacac agtaatggtt gaactcacca
     2221 tcgacggcat caaggttgaa gcacccgaag gcagcatggt catccaggcc gcgcacaagc
     2281 ttggcgtgta tgtgccgcac ttttgttatc acgaaaaact gacgattgca gccaattgcc
     2341 gcatgtgcct ggtcgacgtt gaaaaggttg gaaaaccgtt gcctgcctgc gccacgccgg
     2401 tcacgaatgg catgattgtg catacgcact cgcagaaagc catcgatgct cagaaaagcg
     2461 tcatggaatt cttgctgatt aaccatccgc ttgattgccc cgtgtgcgat cagggtggcg
     2521 agtgccagtt gcaggatctg gcggtagggt acggcgcgtc ggcatcgcgc tacaaagaag
     2581 aaaaacgcgt cgtcgtgcat aaagaaatcg gcccgctggt gtcggcccag gagatgcagc
     2641 gatgcatcca ctgcacacgc tgcattcgtt tcggcatcga gattggcggc ctgatggaac
     2701 tgggcatggt caatcggggc gaacgttccg agatcacttc ctttgtgggg cgcgcggttg
     2761 aatccgaatt atcgggcaat atgattgatg tttgcccggt gggcgccttg acgtcggctc
     2821 cgttccgttt tgccgcccga acgtgggaac tggcgcggcg ccgttcggtc agcccgcacg
     2881 atagcctggg cgccaatctg gtcgtgcagg tcaagggcga gcgcgtgatg cgtgtggttc
     2941 ctttcgataa tgaagacgtg aacgaatgct ggatcagcga ccgcgaccgg ttcgcctacg
     3001 aaggcctgta caccgaggat cgccttgctc atcccatgat caaaggtccg gacggcgatt
     3061 ggcgcgaagc ttcctggagc gacgctttgc agcaagttgt caaaggtttg aataacgtgc
     3121 gcgatcagta cggcgccgaa caaattggcg cgctgtccag tcactatgcc acgaccgagg
     3181 aattcgccct gctggcgcgc ctgacgcgcg ccctgggttc ggaaaacgtc gatttccgct
     3241 tgcgttatat ggatcccggc tttgaccagg ccgttcccgg cgtgccctgg ctgggcttgc
     3301 cgattgccga gctgaaccaa ctggatcgcg tgctggtggt gggctctttc ctgcgcaagg
     3361 atcatccgct gatgactcag cgcctgcgtc aggcggccaa gcgcggcacg caaattacgc
     3421 ttatcgaatc cgccgccgat gatcccttgt tgcccgttgc ggcgcgcctt acggtcgctc
     3481 ccagtttgtt gcccaatgcg ctggccgaag ttctggtcgc ggtggcgcgt gccaaacaac
     3541 agccggttcc ggccgaactg gcgggcgttg aaccctctga cgcggccaac cagattgccg
     3601 ccagcctgac ttcaggcgca aaaacggcgg ttctgcttgg caatatggcg gtgacttcga
     3661 atcaggcttc tttgatcgcc gccaacgcgt ttgcgctggc taaagcggtt tcggcccgat
     3721 ttggatttct gaccgtcggc ggcaatacag tgggcggcta tctggccgga gccatcccgc
     3781 gtcgcggggg cttgaatgct gaacaaatgc tggaccagcc tctgaaggca tatctggtca
     3841 tgcatgccga gccttttctc gatgccgata acggcgtgcg cgccaccgag gttttgaagt
     3901 cggccgattt cgccgtggcg ctgacatcat atcgttcggc cgcacaggac tgggccgatg
     3961 tcatgctgcc tatcgcgccc tttactgaaa cgtcgggcag ctttgtcaat gccgaaggtc
     4021 gcgtgcaaag cttcaaaggc acggtcgcgc cgttcgtcga tacccgcccc gcctggaaaa
     4081 tattaagggt gctgggcaac ttgttccagt tgcagggatt cgacgacgaa acatccgaat
     4141 ccgtgcgcga ttccattatg gtgggtggca tcgaaagccg tttgtccaac gacgttgtcg
     4201 gcccggtagg cacaagcgcg aaacttgacg gcctcgagcg ggtcgccgat gttccgattt
     4261 atcgcagcga ttccatggtg cgccgttcag cgcctttgca attgcccccg gtctcgcaac
     4321 cgcccaaagc gcgcatgacg gccgatacgc tggcgagtct gggtgtgcag gcaggcgaga
     4381 tggttcgtgt gcgttcggcg caaggtgaaa ttcttttggc ggcgcagcac gacgatactg
     4441 tggcgcccgg gtgtgtgcgc attgcggccg ccttcgccga aacggcgcct ttaggtggcg
     4501 cgttcggtca acttacggtg gagcgcgcct gatatggatt ggctgaaagt cgttgaaaat
     4561 tacggcacgg gactggtggg gcctacggcg tggctggtca tttggatact ggtcaagatt
     4621 gtcgtgatcg cggtgcccgt tattttgtgc gttgcttatc tgacgtactg ggaacgtaaa
     4681 ctgataggcg ccatgcacgt gcggctgggg ccgaccaagg tcggttacaa gggcttgttg
     4741 cagccttttg ccgacgtttt caaactgctc accaaagaag tgattgttcc caccaaggcg
     4801 aataaggtac tgtacatatt ggctccggtc gttacgctta t
//
LOCUS       DATCXU010000230         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1149816,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000230 DATCXU010000000
VERSION     DATCXU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149816"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VIR04_05175"
     CDS             complement(<1..280)
                     /locus_tag="VIR04_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY9460170.1"
                     /translation="MGVAIGQTGIDRIGDPARQPQALTTPYLFRQATAPHIAAALENR
                     PMSLPHILECYERIAERADAVVVEGVGGFCVPLNGSTDTADMARQLGLP"
     gene            173..433
                     /locus_tag="VIR04_05180"
     CDS             173..433
                     /locus_tag="VIR04_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460171.1"
                     /translation="MRGSGLPEQIRCCQGLRLAGWVANTVDPGLPNSDANVATLRARI
                     DAPLLGRVPRLFPGSAPAVQELVNAAAEYLDFSRLPEWPAAR"
     gene            complement(483..1490)
                     /locus_tag="VIR04_05185"
     CDS             complement(483..1490)
                     /locus_tag="VIR04_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460172.1"
                     /translation="MPLNPRSWSASFVAGLIAFAAFSAAVPLAAAEDFPSRPLTFIVP
                     YSPGGPLDTMSRLLAKKVQPLLGQTVIVENRPGAGGNIGAGLAAKAKPDGYTLVMGAV
                     ATNAINPWLYPSLPFDPIKSFAPVTLVAAVPNVLIVNNEFASKNHIDSVKDLIAFAKA
                     HPGKLNFASGGNGSAGHLAGALLNVRAGIDTLHIPYQGASPAKLSLLGGTTDFMFDNL
                     ASAATVIKDGKVKALAVTTAERSSIFPELPTMQQQGVKDFDLGTWFGVFTTGGTPRDI
                     VDRLNKAYVKALEDPEVRQSLLIMGSDAKPTTPDAFAELVKNDLAKYKDIVKVSGAKV
                     F"
     gene            1710..>2182
                     /locus_tag="VIR04_05190"
     CDS             1710..>2182
                     /locus_tag="VIR04_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY9460173.1"
                     /translation="MTSPDHPVLSARTRYRTIGLFSRLLLCGLMALLTLTITPACAQP
                     SDTKISSEPAVDIDTVLGGARAKLDEVQKGLKKPQDSATLLQFRAAALAAQTQAEAAA
                     TRIAPEMVSVKARLAQLGAPQPGAPEAPDVAAQRKELSKNSGKLDAQNKLAKLLSV"
BASE COUNT          485 a          663 c          622 g          412 t
ORIGIN      
        1 ccggcaagcc aagttgccgc gccatatcgg cggtatcggt cgaaccgttt agcggtacgc
       61 aaaagccacc gactccttcc accacgaccg cgtcggcgcg ctctgcgatc cgctcatagc
      121 attcgaggat atgcggcaaa gacatagggc ggttttcgag tgcggcggca atatgcgggg
      181 cagtggcctg ccggaacaga tacggtgttg tcagggcctg cggctggcgg gctgggtcgc
      241 caatacggtc gatcccggtc tgcccaatag cgacgccaat gtggcgacac tacgcgcgcg
      301 tattgacgcg cccctgctgg gccgggtgcc gcgactgttc ccgggatcgg cgcccgccgt
      361 gcaggagctg gtcaatgcgg ccgcagaata cctggatttc tccaggctgc ccgaatggcc
      421 tgccgcccgg taaaaaaagc ctcattcgga aggactcttt tgacgcaaga gccttccccg
      481 acctaaaaaa ctttcgcgcc cgataccttg acgatatctt tatatttggc caaatcattt
      541 ttgaccagtt cagcaaatgc gtcgggtgtc gtgggtttgg catccgaacc catgattaac
      601 aggctttgac gcacctcggg atcttccaga gccttgacgt aggctttatt cagtcgatcg
      661 acaatatccc tgggagtccc gcccgtggtg aatacgccga accaggtgcc caggtcgaaa
      721 tccttgactc cctgttgctg catcgttggc aactcgggaa aaatcgatga tcgctctgcg
      781 gtggtcacag ccagcgcctt gactttgccg tccttgatca ctgtcgccgc cgaagccaga
      841 ttatcgaaca tgaaatcagt ggtgccgccc agcaaggaaa gcttggccgg cgaagccccc
      901 tgatatggga tgtgcaatgt atcgatgccg gcacgcacgt tcaacaacgc gccagccagg
      961 tgaccagcgc tgccattgcc gcccgaagca aaattgagct tgccgggatg agccttggca
     1021 aaagcaatca ggtctttcac tgaatcgata tggtttttgc ttgcaaactc gttattgacg
     1081 atcaaaacat ttggcacggc ggccaccagc gtcacaggcg cgaagctctt gatcgggtca
     1141 aagggcagac tgggatacaa ccaggggttt atcgcgttgg tcgccaccgc tcccatgacc
     1201 agggtatagc catcaggctt ggctttggcg gcaagacccg cgccaatgtt gccgcccgcc
     1261 cctgggcggt tttcgacaat cacggtttgt cccagcaggg gctgaacttt cttggccagc
     1321 agccgcgaca ttgtatccag cgggccgccg ggcgaataag gcacgataaa tgtcaacggc
     1381 ctggacggaa aatcttccgc cgcggcaaga ggaacggccg ctgaaaaggc ggcaaatgca
     1441 atcagccctg ctacgaagga cgcggaccat gatctgggat taaggggcat gaaaaatcct
     1501 ctttaaaaga gattacgcaa tatccggaaa ggcccaatgc taaccccgct ttcgcccgcc
     1561 ggtctactcc tttgtggacg ccaaccgtcg cggcgcaaag aaaacacagc cgcgcggtaa
     1621 cacacgttac cgcatctcgg gcgaggcctt aaggcatagt atgatgtgcc ctgtcgacgt
     1681 gagtttcccg cctttccagc ataagcccga tgacatcacc cgatcatcca gtcctttcgg
     1741 cgcgaacccg gtaccggacc attggcctgt tcagccgttt gctgctctgt ggcttgatgg
     1801 cgctgctgac gctcacgatc acgccggcgt gcgctcaacc cagcgacacc aagatcagca
     1861 gcgaacccgc cgtcgacatc gatacagtcc ttggcggggc acgcgcaaaa ctcgacgaag
     1921 tgcaaaaggg cctgaaaaag ccacaggaca gcgccacact gctgcaattt cgcgccgccg
     1981 ctttggcggc gcaaactcaa gccgaagcgg cggcgacccg cattgcccct gaaatggtca
     2041 gcgtgaaagc acgcctggcc cagttgggcg ccccgcagcc gggcgcgcca gaggcgccag
     2101 acgtcgcggc gcaacgcaaa gaactaagca aaaacagcgg caaactggat gcgcagaaca
     2161 agctggccaa actgctctca gt
//
LOCUS       DATCXU010000231         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1122956,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000231 DATCXU010000000
VERSION     DATCXU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122956"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VIR04_05195"
     CDS             <1..1726
                     /locus_tag="VIR04_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY9460174.1"
                     /translation="WRAQQEQLWCAPLQTTVNRAFLQLRRRERDGLMLSSEANLARNG
                     WKQLQTLCSDKAGAELDTRATLETLVQHPLPADATAVDDPLAQHPQPLTAWQVALEQQ
                     PRMAARRVRVTEAGKNRDQPWYEDIESNISIGYNIQDRSGVTTQGHGLVASLNFLMPL
                     EVGSHSSARHQANVARYISAMKQLLAERRNLMVELGQSLRLQREARQELESRDEELAA
                     AKLLLNDRRARSSLDEEGSYSQIQDAQRQIYQAGFARIAAWHRLWLQDASLRLLVDGD
                     PQADVLLGAQRVRWLGQAAQGAAAADPVGTTVAAGPPRATASSWSQGVYVWDSAALLD
                     STRRTTELMALGGAGMRQIYLGLNAAQLGQLTQTKQSLAALLEEARTRNLRIGLLLGD
                     SAWINPSDRGQLIALIGSLKELPFSSLHLDLEVEQLGVPVPDDHLQNWVDTVAAAVKA
                     SPWPVEISSHYRWFAAPKVGQTCVPCELSRMRVAGVSLMIYTRNPKRSTELAAQIARR
                     WPSLSFRLAQSVESQLTPQESWAGVSPNQLDEQEQRWRAKLEPLGVTGIDWQDWHSYP
                     RFTQSAQR"
     gene            1723..2844
                     /locus_tag="VIR04_05200"
     CDS             1723..2844
                     /locus_tag="VIR04_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY9460175.1"
                     /translation="MKVRFDSSKERHPMQEQGLQVLYGSGKRTAFRLRWYLILFLVAS
                     PLIWFVGNLAYGAFVVEAPAQLLLPILEIRARDAAQISRLAVRDGEQVHSGQLLVQMD
                     NPEWRQRLNQLNALSLQSSASTQSASKSLQNVLRRQLGRAQQRYSLAQSLLKDGAATN
                     AEVLAAAAERDRAQAALYSFEQQQDQERQQPGLQRDIALQDAEARSLQNRLEALRVVA
                     PETATVVDVPVNEGENVGPGTLLMRLERPGKPSIWVYLQPADAAKYGQPGQSLELGLP
                     DGTWLNAHVEDAADRTRRLPDELREPFSAAQRGLLVSVTVDGEFPKRWLVDHLTLRAR
                     FSHDFSDVYSWWARVRNSLKGHHDFSPHATAFDTRHIRQ"
     gene            2924..>3224
                     /locus_tag="VIR04_05205"
     CDS             2924..>3224
                     /locus_tag="VIR04_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY9460176.1"
                     /translation="MTPLRILHSEAATSFGGQEHRIYNEMLAMRDRGHSLEAICQPEA
                     LLTQRLRAQGFVVHTTSMDGPANFARSVIKVRSILIAGRFDVLNCHSRRDTLIAAT"
BASE COUNT          658 a          915 c         1011 g          640 t
ORIGIN      
        1 gtggcgagcg caacaagagc agttgtggtg cgcgccattg cagacaacgg tcaaccgcgc
       61 ctttttgcaa ttgcgccgtc gtgaacgtga tggcttgatg ctgtcctctg aagccaatct
      121 ggcgcgaaac ggatggaaac agctccagac gctgtgttcg gataaagcag gagccgaact
      181 ggatacgcgc gccactctgg aaacgcttgt ccagcatcca ttgccggccg atgccacggc
      241 ggtcgacgac ccgttggctc agcatccgca acctttgaca gcgtggcaag tggcgcttga
      301 gcagcagccg cgcatggcgg cgcgcagggt gcgcgtgacc gaggcgggca aaaatcgcga
      361 ccagccgtgg tatgaggaca tcgaatcaaa catcagcatc ggatacaaca tacaggaccg
      421 cagcggggtg acgacgcaag gtcatgggct ggttgccagt ctgaattttc tgatgccgct
      481 ggaggtaggc agtcattcat cggcgcgcca ccaggccaat gtggcgcgct atatttcggc
      541 catgaagcag cttctggcgg agcgccgcaa ccttatggtc gagttgggcc aaagtctgcg
      601 tttgcaacgc gaagcgcgcc aggaactgga aagccgcgac gaggagctgg ctgccgcgaa
      661 actgctgctg aacgaccgcc gcgcacgcag cagcctggac gaagaaggca gctattcgca
      721 gatacaggac gcccagcggc agatctatca ggccggattt gcgcgcattg ccgcctggca
      781 tcgattgtgg ttgcaggatg cgtcattgcg tttgcttgtc gatggcgatc cgcaagcgga
      841 tgtcctgctc ggcgcgcagc gcgtgagatg gctggggcag gctgctcagg gcgccgcggc
      901 ggcggacccg gtcggcacga cggtggccgc ggggccgccc cgcgccacag cttcgtcatg
      961 gtcgcaaggc gtatatgtat gggacagtgc cgcattgctc gattccacgc gcagaacgac
     1021 cgaattgatg gcgctgggag gcgccggcat gcggcaaatc tacctgggtc tgaatgccgc
     1081 ccaactgggc cagttgaccc agacaaaaca atccctggcg gcacttcttg aagaagcccg
     1141 cacgcgtaac ttgcgaatcg ggttgttgct gggggattcc gcgtggatca atccctctga
     1201 tcgcgggcaa ctgattgcgc tcatcggctc gttgaaggaa cttccgtttt ccagcctgca
     1261 cctcgatctg gaagtcgagc aacttggtgt tccggtgccg gacgatcacc tgcagaattg
     1321 ggtcgatacc gtggccgctg ccgtcaaggc cagcccctgg ccggtcgaaa tatccagtca
     1381 ttatcgctgg ttcgccgccc ccaaggtagg gcagacctgt gtgccttgcg agctgtcccg
     1441 gatgagagtg gctggcgtta gcctcatgat atacacgcgc aaccccaagc gcagcaccga
     1501 gttggcagcg caaattgcgc ggcgttggcc gagtttgtct ttccgcctgg cgcaaagcgt
     1561 ggagtctcaa ttgacaccgc aggaaagctg ggcgggggtg tctcccaacc agcttgatga
     1621 gcaggagcaa cggtggcgcg ccaagctgga gccgctcggg gtaactggca ttgattggca
     1681 ggattggcat tcctaccctc gttttacaca gagcgctcaa agatgaaagt ccggttcgac
     1741 agcagcaagg aacgtcatcc gatgcaagag cagggcctgc aagtcttgta tggttcgggt
     1801 aaacgcacgg cctttcgctt gcgctggtac ttgattctgt ttttggtggc gagcccgtta
     1861 atatggttcg tggggaacct ggcttacggc gcgtttgtag ttgaggctcc cgcgcaattg
     1921 ctgttgccca tcctggaaat tcgagcacgc gacgctgccc agataagccg cctggccgtg
     1981 cgcgatggcg aacaggttca cagcgggcag ttgctggtgc aaatggataa tcccgaatgg
     2041 cgccagcgcc tgaatcagtt aaacgctcta tcactgcagt cgagcgcttc gacgcaatcg
     2101 gccagcaaga gtctgcaaaa tgtactgagg cgtcagcttg gccgcgccca gcagcgctat
     2161 agcctggcgc aaagcttgct taaagatgga gctgccacta acgcggaagt tttggctgcc
     2221 gccgccgagc gcgatcgggc gcaagccgcg ctttactcgt ttgaacaaca acaggatcag
     2281 gagcgccagc agcctgggct gcaacgcgat attgcattgc aggacgcgga ggcgcggtca
     2341 ttacagaatc gcctggaagc ccttcgggtg gtggcgcctg agacggcgac ggtcgtggat
     2401 gtcccggtaa acgaaggcga gaacgtgggc cctggcacgc tgttgatgcg gcttgagcgc
     2461 cccggcaagc catcgatctg ggtttacctt cagcccgccg atgcggccaa gtatgggcaa
     2521 cccggccagt cgctggagtt gggtttgccc gacgggacat ggctgaatgc acatgtggag
     2581 gacgcggctg accgcacccg tcgtctgccc gatgagctgc gcgagccatt tagcgccgcc
     2641 caaagaggct tgctggtgtc ggtgacagtc gatggcgaat ttccaaagcg ctggctggtc
     2701 gaccatttga ctttgcgagc gcgtttttcg catgattttt cggacgtgta ctcgtggtgg
     2761 gcgcgcgtgc gcaatagcct gaaggggcac catgacttct cgccgcatgc aaccgccttt
     2821 gacaccaggc acatcaggca ataattcaaa taggctgttt cagttcgttt caacccgttt
     2881 tataactatt gaagccgcgc atcaaaaatt cgatactcgc ttcatgactc cattacgcat
     2941 actgcattcg gaagcggcaa cgagtttcgg cggccaggag catcgcatct acaacgaaat
     3001 gctggcgatg cgcgaccgcg gtcatagtct ggaagcaata tgccaacccg aggctctatt
     3061 aacgcagcgc cttcgtgctc aagggttcgt cgtgcacacc acttcaatgg atggcccggc
     3121 caactttgca agaagcgtga tcaaggtgcg ctccatcctg atagcaggcc ggttcgacgt
     3181 actgaactgt cacagccgcc gcgacacctt gatagcggct actg
//
LOCUS       DATCXU010000232         5222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_558880,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000232 DATCXU010000000
VERSION     DATCXU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558880"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIR04_05210"
     CDS             <1..191
                     /locus_tag="VIR04_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9460177.1"
                     /translation="AHVTQCPYCIRNNVERVVKLDVTEQEIMEAIWVAAEMRAGAALA
                     HSTLALDAINHAEGQHSF"
     gene            710..1594
                     /locus_tag="VIR04_05215"
     CDS             710..1594
                     /locus_tag="VIR04_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY9460178.1"
                     /translation="MLVLPDFLLIALGWALLHKFKFSREFFQSAERLVYFVLFPALLF
                     HSITQTPISIASATTLLQATVAAMGFGVALAWLAVPVLGPDPVDHASTAQCAFRFNTY
                     IGLSIALGLGGTKGQTIMAIIVGFAVPISNMAAVHALARQNGGKALKEMAKNPFIIAT
                     VLGLLVNLLSVPVPKPVDVVLGRLGISAIAIGLLCVGATLSLRGGIAAGRLMSWMITA
                     RLVINPIGAIFIGWALQLPLLERQILLLFCALPPSSTAHVLAARMGGNGSLTATTMSI
                     STLLSAMTIPFWLMISSH"
     gene            1609..2136
                     /gene="tsaE"
                     /locus_tag="VIR04_05220"
     CDS             1609..2136
                     /gene="tsaE"
                     /locus_tag="VIR04_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY9460179.1"
                     /translation="MQNSPILASASLYLADEAATNALAARFAPLLCATHKALATKTPG
                     GRIHLRGDLGAGKTSFVRAMLKATGVTGRIKSPSYTLLETYKVSNIYFYHLDFYRFKD
                     QYEWQDAGFRDILQKEAVVLIEWPEQAGAALPPPDLEIKLSYAGTGRQASLAAYTTRG
                     KLWLTILALAPQESL"
     gene            2094..3446
                     /locus_tag="VIR04_05225"
     CDS             2094..3446
                     /locus_tag="VIR04_05225"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY9460180.1"
                     /translation="MADDSCPRTTGIPLNIYQRRRFLATSASLLILPLIPRTTMAATI
                     LAVRTWPADEYTRVTLELDSELKVEHFMLENPNRMVVDINGLTMSKAVNQLVSKIKPN
                     DPYIASIRAGQNRPNVIRLVIDLKQSVAPQIFTLKPVGAYKYRLVLDLYPKVAQDPLM
                     ALTEASGDQDPLASVLEQLARNSNDNAPIPSVPGQRLPPKTVLKKPEPSAPPPLGIDR
                     PILVALDPGHGGEDTGAIGPGGTREKDVVLAIGRRLKKLIDSQPNMRAYLTRDNDYFV
                     PLNVRVQKARRVQADLFISIHADASPNRSAKGSSVFALSQHGATSTAARWMAKRENDA
                     DLIGGINLGAHDQQVAKVLLDLSTAAQISDAMSIGLRVLTEMGKINALHGHKVERAGF
                     AVLKAPDIPSILVETAFVSNPTEERLLRSPTHQEKIANAMLSGVKGYFAAHPALARRA
                     "
     gene            complement(3449..4096)
                     /locus_tag="VIR04_05230"
     CDS             complement(3449..4096)
                     /locus_tag="VIR04_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY9460181.1"
                     /translation="MLDFLNLVLHIDQFLGDWVAQYGDWIYLVLALIVFGETGLVVLP
                     FLPGDSLLFIAGAFGASGLINPVLLGVLLIAAAVLGNTVNYSVGRYIGPRVFSSNFRF
                     LDHNALIKTHAFYEKHGGKTIVLSRFLPIIRTFAPFVAGVGSMDFLRFQLFNISGAVL
                     WVTGLIALGYFFGNVPIIKAHLNTIVLIGVGAAIVPVVLGTLWKLFKKPAAPGRG"
     gene            4251..>5222
                     /gene="mutL"
                     /locus_tag="VIR04_05235"
     CDS             4251..>5222
                     /gene="mutL"
                     /locus_tag="VIR04_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY9460182.1"
                     /translation="MTERRAIAPLPDRLISQIAAGEVIERPASVLKEVLENAIDAGAC
                     AVEVRLDGGGIRRISVTDDGAGIPDTELALALTRHATNKISSLADLESVASMGFRGEA
                     LASIASIAQLTLTSRTASAPHAWQLSAGAKAPSAASGPLGTTTEVCQIFDNVPARRKF
                     LRSEATEYGHCVDALERIALAYPHIAFRLFHNNKPQRHWRATELLQRVRDVLGAEFFE
                     QSLGVKLDHGLISLQGLITRPAYTRNRADRQYLYVNGRYVKDRSVSHAIRHAYADVLH
                     GDRQPGYVLFLSIDPAAVDVNVHPTKHEVRFRDSGAIHRFVAQTLGQT"
BASE COUNT         1207 a         1550 c         1372 g         1093 t
ORIGIN      
        1 tggctcacgt cactcaatgc ccgtactgta tccgcaataa tgttgaacgg gtcgttaaat
       61 tggatgtgac cgagcaagaa attatggaag caatttgggt agcggccgaa atgcgtgctg
      121 gcgcggcttt ggcccattcc actcttgccc tggatgcgat taatcacgcc gaaggccagc
      181 acagctttta gcaagtgacc ttgtcgtagt accgtaacca atcacacagc gatgggcata
      241 cctaaagccg gcccaacatg ggtgcacacc aactggcgtc tccagcagga atcgaacctg
      301 caatcttccc ttcataccac tacggttttc accgcccaag aggggtttgt ggtctggact
      361 ctgtcttaac catggctatt gccgcaggtt gcgcctgtcg agtctctaca cgttcctgca
      421 ttgcaggctt cgctcggtgt tgcctcgtgt tatgcacaca gggggttcgc cgacgttaag
      481 cgcattcacg ctggagcttt cgcaaccagg tgcccaaatt caggagggga aagttatatc
      541 catttaacta tggagaccgt gtgctttacc gtctttcgct aaaaaatctt atcgccgaat
      601 aatcaggctg ctattttagg ctaagcttat tcggttcagg cgcaacaata acagtaattg
      661 gtgatccctg cctgcggcct aaaccacact atcgctcatg tccgttttca tgcttgtttt
      721 acccgacttt ttgctgattg cgctgggatg ggcgctgctt cacaaattta agttttcacg
      781 tgaatttttt caaagcgccg agcggctggt ttatttcgtg ctattccccg ccctgctgtt
      841 tcattccata acgcaaaccc ccatcagtat cgctagtgcg acgacactgc tgcaagctac
      901 tgtggccgcg atgggattcg gcgtagcgct tgcctggctg gccgttcccg ttttagggcc
      961 ggatccggtc gatcatgcgt cgacagcgca atgcgccttc cggttcaaca cgtacatagg
     1021 cctgtcgatc gcgctgggcc tgggcggaac gaaaggtcag acaatcatgg ccattatcgt
     1081 gggctttgcg gtgcccattt cgaacatggc ggccgtccat gcgctggcgc gccaaaatgg
     1141 cggcaaggcg cttaaagaaa tggccaaaaa tcccttcatc attgccaccg tcctgggact
     1201 gctggtcaat cttttgagcg tgcccgttcc gaaacccgtt gatgtggttc tggggcgtct
     1261 ggggatctcc gccatcgcca tcggcttgct gtgcgtcggc gccaccttgt cgttgcgagg
     1321 cggaattgcc gcaggcaggc tgatgagctg gatgataacc gcgcgcctgg tcatcaaccc
     1381 gatcggcgcc atttttattg gttgggcgct gcaattgccg ctgctggagc gccaaatact
     1441 gttgttattt tgcgcgttgc cgccatcgtc gaccgcgcac gttctggccg ccagaatggg
     1501 cggcaatggc tctttgaccg ccaccacaat gtcgatcagc accctgctat cagcaatgac
     1561 aatcccattt tggctgatga tcagcagcca ctaaattggc accatattat gcaaaatagc
     1621 cccattttag cctctgctag cctgtatttg gcagacgaag cggccacaaa cgcgctggcc
     1681 gcccggtttg cgccgctcct ttgcgcaacc cacaaggcgc tcgccactaa aaccccgggc
     1741 gggcgaatcc acctgcgcgg agacctcgga gcgggcaaga ccagcttcgt ccgcgccatg
     1801 ttgaaagcca ctggtgtaac gggccgaatc aaaagtccaa gctatacttt acttgaaacc
     1861 tataaagttt ctaacatata cttctatcat cttgatttct atagatttaa ggatcaatat
     1921 gaatggcaag atgccggatt ccgggatatt ttgcaaaaag aggcggtagt tttaattgaa
     1981 tggcccgagc aggcgggcgc ggcattgccg cctcctgacc ttgaaataaa actaagttac
     2041 gcgggtaccg gcaggcaggc cagcttggct gcatacacca ccagaggaaa attatggctg
     2101 acgattcttg ccctcgcacc acaggaatcc ctttaaatat ataccagcga cgtcgattcc
     2161 tcgcgacgag cgcctcactg ctgattctgc ccctcattcc ccgcaccacc atggcggcaa
     2221 ccattctggc cgtgcgcaca tggccggccg atgaatatac ccgcgtcacc cttgaattgg
     2281 atagcgaatt gaaggtcgag catttcatgc ttgaaaaccc gaatcgcatg gtggtcgata
     2341 tcaacgggct gacaatgagc aaggccgtca accagttagt gtccaaaata aagcccaacg
     2401 acccctacat tgcatcaatc cgcgctggtc aaaaccgccc aaacgtcatc cgtctggtta
     2461 ttgacctgaa acaatcagtc gcgccccaga tctttaccct gaaaccggtc ggagcttaca
     2521 aataccggct ggtgctcgat ttgtacccta aagttgcgca agacccgttg atggccctga
     2581 ctgaagcaag cggcgaccag gaccctttgg cgtcggtgct tgaacaactg gccagaaact
     2641 cgaacgacaa cgccccaatt ccctcggtgc cgggacagcg attgcccccc aaaaccgttc
     2701 tcaaaaaacc agaaccctcc gcgcctccgc ctctgggcat tgaccggccc atactggtcg
     2761 cgctggatcc aggacatggc ggtgaagata ccggcgccat cggcccgggc ggcacacgcg
     2821 aaaaggatgt cgtgctggca attggccggc gcctgaaaaa actgatcgac tcccagccca
     2881 atatgcgcgc ttatctgacc cgggacaacg attactttgt gccgctgaac gtcagggtgc
     2941 aaaaggcgcg ccgggtgcag gccgatctgt tcatttccat acacgccgac gcgtcgccca
     3001 accgcagcgc aaagggctcg tccgtttttg cgttgtcgca gcacggcgcc acgagcaccg
     3061 cggcgcgctg gatggccaaa cgggaaaacg acgccgactt gatcggcggc attaatttag
     3121 gcgcccatga ccagcaagtg gccaaagtct tgcttgatct atcgacggca gcccaaatca
     3181 gcgatgccat gagcataggc ctgcgcgtgc ttacggaaat gggaaaaatc aatgccttgc
     3241 atggccacaa ggttgagcgc gccggattcg cggtgctcaa agcgcccgac atcccttcta
     3301 ttctggtgga aacggctttc gtcagcaacc ctaccgaaga aaggctttta cgcagcccca
     3361 cgcaccagga aaaaatcgca aacgcaatgc ttagcggagt taaaggctat ttcgccgccc
     3421 atcccgcatt ggcccgccgc gcctgaattc aaccacggcc gggcgccgcg ggttttttaa
     3481 acaacttcca gagcgtgccc agcaccacag ggacgatggc cgcgccaacg ccaattaaca
     3541 caatcgtatt caggtgcgcc ttgataatgg gtacattccc aaagaaataa cccaacgcaa
     3601 tcaggcctgt gacccacaac acggcgccgg aaatattgaa taactgaaac ctgaggaaat
     3661 ccatgctgcc gacgccggcc acaaaggggg cgaaggtgcg gataataggc aaaaagcgcg
     3721 acaggacaat ggttttgccg ccgtgttttt cataaaaggc gtgtgttttt attagtgcat
     3781 tatgatccag aaagcggaaa ttgctggaaa aaacacgcgg gcctatatag cgcccaaccg
     3841 aataattgac ggtattgccc aataccgccg ccgcaatcag caatacgcca agcaacaccg
     3901 gattaatcag gccggacgcg ccaaacgcgc cggcaatgaa tagcaatgaa tcgccgggca
     3961 gaaagggcaa gacgaccagc ccggtctcgc caaaaacgat taacgccagc accagataaa
     4021 tccagtctcc atactgggcg acccaatcac ccaggaattg gtcgatatga agcacaaggt
     4081 ttagaaaatc aagcatagac gaaccactga agaaagatat ggcaatatag cccactctcg
     4141 ctgcgcctgt gcgcgcgatg catccaggcc gctaaaatcg ctattatgaa gcgtttaaag
     4201 cctggcgcgc ccgctctgcg ccgcgcatgc ttgcaaccgg aatactgttt atgactgaac
     4261 gtcgtgccat cgccccactg ccggacaggt tgatcagcca gatcgcggca ggcgaagtca
     4321 tcgagcgacc cgcctcggtg ctgaaagaag tgctggaaaa cgccattgat gccggcgcct
     4381 gcgccgtgga agtccgcctt gacggcggcg gcattcgccg catatctgtc acagacgatg
     4441 gcgccggcat tccggacacc gagctcgccc tggcccttac acgccatgcc accaacaaaa
     4501 tctccagcct ggccgatctc gaatccgtcg cttcaatggg ttttcgcgga gaggcgctgg
     4561 cctcgatcgc atcgatcgcc caactgactt tgacttcgcg gacggcctcc gcgccccatg
     4621 cctggcaatt aagcgcgggg gccaaggcgc ccagcgccgc ctcggggccg ctgggaacaa
     4681 ccacggaagt ctgccagatc tttgacaacg tgcccgcgcg acggaaattc ttgcgtagcg
     4741 aagccaccga atatggccac tgcgtcgacg ctctggagcg tatcgcgctg gcctatccgc
     4801 atattgcctt ccgcttgttc cacaacaaca agccgcaacg ccattggcgc gccaccgaac
     4861 tgctgcaacg cgtgcgcgac gtgttgggcg ccgagttctt tgaacaaagc ctgggcgtca
     4921 aacttgatca cggcttgatt tcactgcaag gcctgatcac gcggcccgcc tatacgcgca
     4981 accgggcaga tcggcaatat ctgtatgtca atggccgcta cgtaaaggat cgcagcgttt
     5041 cccatgccat acgccatgcc tacgccgacg tgttgcacgg agaccggcaa ccagggtatg
     5101 tcctgtttct gtcgatcgac ccggcggcgg tcgacgtcaa tgttcacccg accaaacacg
     5161 aggtgcggtt ccgcgacagc ggcgcgattc atcggtttgt cgctcaaaca ctgggccaga
     5221 cg
//
LOCUS       DATCXU010000233         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_165414,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000233 DATCXU010000000
VERSION     DATCXU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165414"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2785)
                     /locus_tag="VIR04_05240"
     CDS             complement(<1..>2785)
                     /locus_tag="VIR04_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9460183.1"
                     /translation="MVSLPVSQYPTIAPPAVQIRAMYSGASAKTVEDTVTQVIEQQMS
                     GLDHLLYMSSTSDDSGTAQITLTFASGTNPDVAQVQVQNQLQLAAPLLPQAVQQAGVK
                     VSKSSSSFLMVMAFVSTNHSMTKYDLANYLASRVQDPVSRVNGVGTTTVFGTQYAMRI
                     WLDPNKLTRVNLTPVDVVTALRGQNVQIAAGQLGGTPAAPGQMLTATISEATLLRTPD
                     QFGDVLLKVNSDGSQVRVKDVARIELGGENYIVDTKYSGVPAAGLGVQLATGANALQT
                     ANAVRARVKELSKYFPPGLQVEYPYDTTPFVKISIEEVAKTLFEGIALVFLVMYLFLQ
                     NFRATLIPTIAVPVVLLGTFGIMAVVGFSINTLSMFGLVLAIGLLVDDAIVVVENVER
                     VMAEEGLSPRDATRKAMGQITGALVGVALVLSAVFVPMAFSGGAVGAIYRQFSLTIVA
                     AMVLSVLVALILTPALCATLLKPIPKGHHVQKKGFFGWFNRVFDNSRVKYHTSVDHVI
                     KRPRRWLLLYVLLIAIVGLLFARLPTSFLPDEDQGIMIIQVQTPPGATLERTQKALED
                     VSHYLRDTESAIVQSTFEVAGFNFAGRGQNSGLLFVRLKDWSQRTAAKDKVQALVRRV
                     FAQFAAYKNAVVIPINPPPIPELGTASGFDFELEDRGGLGHNALMAARNQLLADAAKN
                     PNLALVRANGLDDQPQFGVDIDREKASALGVELPAIDQTFSIAWASSYVNNFLDTDNR
                     IKRVYVQADAPFRMNPEDMNYWYVRNMQGAMVPFSSFATGKWTFGSPKLERFNGVSSM
                     EIQGAPAAGRSTGDAMNEIQKIANTLPAGIGYEWTGISFQERLSGSQAPILYALSILV
                     VFLSLAALYESWSIPFSVIMVVPLGIIGALLAATGRGLSNDVYFQVGLLTTVGLSAKN
                     AILIVEFAKELHT"
BASE COUNT          656 a          842 c          732 g          555 t
ORIGIN      
        1 tgtgtgtgca attccttcgc gaactcgaca atcaaaatgg catttttcgc cgataacccc
       61 accgtagtca gcaacccgac ctggaaatac acatcgttgg acaagccgcg cccggtcgcc
      121 gccagcaaag cgccaataat acccagaggc accaccatga tcactgaaaa tggaatggac
      181 caactctcgt ataacgcggc cagactcaga aacacgacaa gaatcgaaag tgcataaagg
      241 atcggcgctt gcgagcccga cagtctttcc tggaacgaga taccggtcca ttcataacca
      301 atacccgcag gcagtgtatt ggcgattttc tgtatttcgt tcatcgcatc gcccgtgctc
      361 ctgcccgctg cgggagcgcc ctgtatttcc atcgacgaga cgccattgaa acgttccagc
      421 ttgggcgagc caaaggtcca cttgcctgtg gcgaacgatg aaaacggcac catggcgccc
      481 tgcatattgc gcacatacca gtaattcatg tcttcagggt tcattcgaaa cggcgcgtcg
      541 gcttgcacat aaacccgctt gatgcggttg tccgtatcaa ggaaattatt aacatacgaa
      601 gaggcccagg cgatggaaaa agtctgatcg atcgccggca actcaacacc cagcgcgctg
      661 gccttctcgc ggtcaatatc aacaccgaat tggggctggt cgtccagccc gttcgcacgc
      721 accagcgcaa ggttggggtt cttggcggcg tccgccagca actggttgcg agccgccatc
      781 aaggcattat gaccaagccc tccacggtct tccagttcga agtcaaaccc ggatgccgtc
      841 ccgagctccg gaataggcgg cgggttgatt gggatgacca ccgcattttt ataggcggca
      901 aactgcgcaa agacccgcct gaccagcgcc tgcaccttgt cctttgcggc ggtgcgctgg
      961 ctccagtctt taagtcgcac aaacaaaaga ccggagttct ggccgcggcc ggcgaaatta
     1021 aagccggcga cctcgaacgt ggactgcaca atggcgcttt ccgtgtcgcg caaatagtgg
     1081 cttacatctt cgagcgcttt ttgcgtgcgc tcaagcgtcg ccccaggcgg cgtttgaacc
     1141 tggatgatca taatgccctg atcctcgtcg ggcagaaacg aggtcggcag acgcgcaaac
     1201 agcagcccca caatcgcgat caataaaaca tacagcaaaa gccagcggcg cgggcgcttg
     1261 ataacatgat ccacgctcgt atggtactta acccggctgt tgtcgaaaac ccgattgaac
     1321 cagccgaaga atcccttttt ctgtacgtga tgccccttgg gaattggctt tagcagcgtt
     1381 gcgcatagcg cgggtgtaag aattaatgcc accagcaccg acaaaaccat ggccgccact
     1441 atcgtcagcg agaactgtcg atagatcgcg cccacggcgc ctcctgaaaa cgccatcggc
     1501 acgaacacgg cggacaacac cagcgctacg ccgaccagcg cgccggtaat ttggcccatg
     1561 gccttgcgcg tcgcgtcgcg cggcgacaac ccttcctcgg ccatcacccg ttcgacgttt
     1621 tccaccacca caatcgcatc atcgaccagc aaaccgatag cgagcaccag tccgaacatg
     1681 gacaaagtat tgatggaaaa accaaccacc gccataatgc cgaatgtgcc cagaagcacc
     1741 accggtaccg caatcgttgg gatcaacgtc gcccggaagt tctgcaaaaa caagtacatc
     1801 acaagaaata ccagcgcaat gccttcaaac agcgttttgg ccacttcctc gatggaaatt
     1861 ttcacaaatg gcgttgtgtc gtacggatat tcaacctgca agccgggagg aaaatacttc
     1921 gaaagttcct tgaccctggc ccgcactgca ttggccgttt gcaatgcatt ggcgcccgta
     1981 gccaattgca cacccagccc ggcggcgggc accccgctgt atttagtatc tacgatgtaa
     2041 ttttcgcccc ccagttcgat acgtgcgaca tccttgaccc gcacctgtga accgtcggag
     2101 ttcactttta gcagaacatc gccaaactga tcgggggtgc gcagcagtgt agcctcgctg
     2161 atggtcgccg tgagcatctg gcctggggca gccggcgtgc cgcccaattg acccgccgcg
     2221 atctgaacgt tctgtccccg cagcgcggta accacatcaa ccggagtcaa gttgacacga
     2281 gtcagtttat tggggtccag ccagatgcgc attgcatact gcgtgccgaa tacagtcgtc
     2341 gtgcccacac cattgacccg gctgaccgga tcctgcacgc gcgaagccag ataattcgcg
     2401 aggtcgtact tggtcatgct gtggttggtc gagacgaacg ccatcaccat caggaagctg
     2461 ctgcttgact ttgaaacctt gacgcccgcc tgctgcaccg cctgcggcag caatggcgcc
     2521 gccagttgca actgattctg cacttgcacc tgagccacat cgggattggt gccagaggcg
     2581 aacgtaagag tgatctgcgc cgtaccggaa tcgtcgctgg tcgacgacat ataaagcagg
     2641 tggtccaacc cgctcatttg ttgttcgatc acttgcgtga cggtgtcttc gactgtcttg
     2701 gccgacgcgc cagaatacat ggcgcgaatt tgaacggccg gcggagcgat ggtgggatac
     2761 tgcgaaacag gcagggaaac catcg
//
LOCUS       DATCXU010000234         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_634556,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000234 DATCXU010000000
VERSION     DATCXU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634556"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            580..1053
                     /locus_tag="VIR04_05245"
     CDS             580..1053
                     /locus_tag="VIR04_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460184.1"
                     /translation="MIGEAFMGLNALNAAINVAKGMKDMNDAVVRNAAIIELQQHILN
                     AQAAQQELIKRTEELEKKIARFDAWDAEKKRYQMKDFGGGTIAYELKADEANGQPLHR
                     ICPNCYQERRLSILQPDGRNASKQEMYSCPGCNKHFKFGVAQERNLGARDSRGWT"
     gene            1341..2327
                     /locus_tag="VIR04_05250"
     CDS             1341..2327
                     /locus_tag="VIR04_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460185.1"
                     /translation="MRRREFISLIGGVVISLPIAARAQSESRMRRIGVLMPFTATDPE
                     EKERNAVFERSLQELGWTVGRDLQIDYRSCGGDATAARRYAAELVTRAPDVIMTAGSL
                     TIGPALQATRTIPIVFTNVADPVGAGFVRSLARPGGNATGFTNFEYTTTGKWMELLKQ
                     IAPRVTRVAVLRDPTSAAGIGQFTAIQSVAQSLGVELTPLSVQDVAEIEAGIAAFART
                     DNGGVIVTAGGTGYHRHLFIGLASRHKLPAIYPFRYYALDGGLITYGPDPRDLTKRAA
                     GYVDRILKGEKPADLPVQAPTKYELVINLRTAKALGLNVPVHLQQIADEVIE"
     gene            2685..>3545
                     /locus_tag="VIR04_05255"
     CDS             2685..>3545
                     /locus_tag="VIR04_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY9460186.1"
                     /translation="MTALSRRSLLAGAAALTVAGSVNAPVRAAAPMAGKQGPSFYRYK
                     IGEFEVTTLNEGVVRNATPANLAVNKTLPDIQKALGDAFLPTDHTINQFNVVIINTGR
                     NLVLIDTGFGDNGAPTTGNLLNNMTAAGIDPKNVDTILISHFHGDHINGIRAKAGSLN
                     FPNAEIMVPATDLKFFLDAGEESKAPQPFKGFFAASKRVFEPIAKDVKQFEYGKELVP
                     GITTVDARGHSPGHTAFLVTSGNGKLLVSCDTVNHQILVRNPDWALWADMDQAMAAAS
                     RKRLLDMAAAD"
BASE COUNT          769 a         1004 c         1045 g          727 t
ORIGIN      
        1 tgcggcttgc cacgtttccg cggacgacga ccggccccgg cgcgagcgtg acctcgcgtc
       61 tgatctgcgt catctgctcc catcccgcat ctttgatccg ggcgagatga agaggtcaca
      121 gatgtcgatc tcgctgagac gctttttatc gaaggctttc aagcaacccc caaccccgac
      181 atgtcgtggc gcccattaca tatcgtggtt ccggcggcgc aaccgcgata tgccatacac
      241 ttagagagcg attttaacct tgggcgtcaa ggccttgcgg ccacatttta tttgagcttc
      301 tacgagaggg cttgccgaaa gccgccaaag atttgtttta ggctgccaaa acccaatcca
      361 tgaggatgat tggcggggta agcccggcga tccagaagcc tcgatttcgc gtcgcattac
      421 gtgacgtcca atgtcggcgc tgtaatagtt acggtcgtcc gcgaaatatc gaagtggtgc
      481 ggcatccgtc tgattgctgt atttgcgagg acgctttgcc gctatcgtgc catctgatta
      541 tcgattgaag ccatcgggcg gctatatctc ggaggtgtca tgatcggcga agcttttatg
      601 ggcctcaatg ccctcaatgc cgcgattaat gtggcgaagg gtatgaagga tatgaatgat
      661 gcagtcgtta ggaacgcggc aatcatagaa ctacagcagc acatattgaa cgcgcaggcg
      721 gcacagcaag agctaatcaa acgcacggag gagcttgaga agaaaatcgc tcgtttcgac
      781 gcgtgggacg ctgaaaagaa gcgttatcag atgaaagact ttggaggtgg gacgattgcc
      841 tacgaactta aggcagacga ggccaatggc caaccgctgc accgcatatg cccgaactgc
      901 taccaagaga gacggctgtc aattctccag cccgatggaa ggaatgcctc taagcaagaa
      961 atgtattcgt gccccggctg caataaacat ttcaagtttg gcgtcgcaca agagcgcaat
     1021 ctcggtgcgc gagattcacg cggatggacc taaactcagc ggtccgaaat tatttctgct
     1081 caccttttgt tggaaaaggc tatttaaaaa atgcctgatt tattggcccg ccctcgatga
     1141 cgttataagc cgggtaccgg accgggttct tttcaaaaaa gtttctaaaa attatgtaat
     1201 ttcaatcctg tccgctttca ggggtaaagc ggacttttgc ggcgtgaatg tccgcttctg
     1261 actcaaagcg gacatggccc cttccggttc gccggtttaa gtcggtacga tagtctgcct
     1321 taggacatcg gggtgtgggc atgaggcggc gcgagttcat tagcttaatt ggtggcgtgg
     1381 ttatttcgtt gccgattgcc gcgcgcgcgc agtcggagtc gcggatgcgg cgcatcggcg
     1441 tgcttatgcc attcacggcc accgatccgg aggaaaaaga acgaaacgca gtgtttgagc
     1501 ggagccttca agagttgggc tggacggttg gccgagacct gcaaatcgat taccgctcgt
     1561 gcgggggcga cgccaccgcc gctcgccgat atgcggcaga actggtcacg cgcgcgccgg
     1621 acgtaatcat gaccgccggc agcttgacga ttgggccggc gctacaggcg actcgaacca
     1681 ttccgatcgt gttcacgaat gtagccgatc cagttggagc cggcttcgtc cgaagcctgg
     1741 cgaggccggg cggcaatgcc accggcttca cgaatttcga atacaccacg accgggaaat
     1801 ggatggagct gctcaagcaa attgcgccgc gcgtcacgcg cgtggcggtc ctgcgcgatc
     1861 ccacgtcggc ggcgggtatc ggccaattca ccgcaatcca gtcggtggcg caatcgcttg
     1921 gggtggagct cacgccgctc agtgtgcagg acgttgcaga aatcgaggcc ggcatcgcgg
     1981 cgttcgcgcg caccgacaat ggcggggtga tcgtaacggc gggcggaacg gggtatcatc
     2041 gccatctatt catcgggctt gcgtcgcgcc acaagctgcc cgcaatctac cctttccgtt
     2101 actacgcctt agatggtggc ttgatcactt atgggcccga tcctcgcgat ttgaccaaac
     2161 gtgcggcggg ttacgtcgat cgcatcctca aaggtgaaaa acctgccgac cttccagtgc
     2221 aggctccgac caagtacgaa ttagttatca acctcaggac cgcgaaggcg ttggggctca
     2281 acgtgccagt gcatctccag cagatcgccg acgaggtgat cgagtgagac ggcgatgtgc
     2341 gctaatggcc caaagcggac atagctagtt ggactgctca tgtctgcttt tgacccaaag
     2401 cgggcatgcc gagaaacgca attggtggcg ctattggggt aaattaatag tgcggtagcc
     2461 gccgccaatg cttgtatcca ccgccggaaa agccgaacgc agcttgccca ttcgcccacg
     2521 ttttggtgag gccacgcttt gcggcaagcg gaatttccaa attggcgccg caacgatcaa
     2581 gtgaacgtga ttgacgggac tagcaccctg ttacgcgggg tttttgccgg ttgcactgcc
     2641 atcgcgccgt ccgctgaggg atggcggcaa ccagggagga ttctatgaca gcgctttcac
     2701 gccgtagtct actggcgggc gccgcggccc ttaccgtcgc aggttcggtg aacgctccgg
     2761 tgcgcgccgc cgcgcccatg gccggcaagc aagggccgag cttctatcgc tacaagatcg
     2821 gtgagttcga ggtgaccacg ctcaacgagg gtgtcgtgcg caatgcgacg ccggccaacc
     2881 tggcggtcaa caagaccctg cccgacatac agaaggcgct cggcgacgca ttcctgccga
     2941 ccgaccacac catcaaccag ttcaacgtcg tgatcattaa taccggccgc aatctcgtgc
     3001 tgatcgatac cggtttcggc gataacggcg cgccgaccac cggcaatctc ctcaacaata
     3061 tgacggctgc cggaatcgat ccaaaaaacg tcgacacgat tctaatcagc catttccacg
     3121 gcgatcacat caacggtatc cgcgccaagg cgggatcgct caatttccca aacgccgaga
     3181 tcatggtccc agcgaccgat ctgaagttct tcctcgacgc cggcgaagag tcgaaggcgc
     3241 cccagccgtt taagggcttt ttcgccgcca gcaagcgtgt gttcgagccg attgccaagg
     3301 acgtcaagca gttcgagtac ggcaaggaac tggtgcccgg catcacgact gtggacgcac
     3361 gcggccacag cccgggccac acggcgtttc tcgtcacgtc gggcaatggc aagctgctgg
     3421 tgagctgcga taccgtcaat caccagatcc tggtccgcaa tccggactgg gcactgtggg
     3481 cggacatgga tcaggcgatg gcggcggctt cgcgcaagcg cctgctcgat atggcggcag
     3541 ccgac
//
LOCUS       DATCXU010000235         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_595135,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000235 DATCXU010000000
VERSION     DATCXU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595135"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /gene="hemN"
                     /locus_tag="VIR04_05260"
     CDS             complement(<1..1396)
                     /gene="hemN"
                     /locus_tag="VIR04_05260"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY9460187.1"
                     /translation="MQRRADIDGETVNTAVLLARDPGKSLPNLPISEELVRRFDVSGP
                     RYTSYPTADRFHAEFTESAYIGKLTERSQRLENPPLSVYVHLPFCESLCYFCACNKII
                     TQDHSRSAEYVRYLGKEAAMVASYLGSDRKTVQLHFGGGTPTFLSASELHELMAVLRT
                     HFEFSDDAELSVEIDPRTVTDTTMAMLAGLGFNRTSFGVQDFDPDVQLAIHRIQPLEM
                     VEAAIRASRAAGFKSINADLIYGLPKQSLATFSRTLDNLIRLSPDRIALYNYAHLPGH
                     FKAQRLIQQSDLPSTEERLQIFLMSVERLLEAGYEYIGLDHFAKSDDELNRARLDNSL
                     HRNFQGYTTRAECDLIGLGVSAIGKVGTSYSQSVRNLHTYYYRLDHGQLGTERGVELN
                     AEDQLRRQVIMEIMCSKPVDFALISQRHGVDFADYFSSELATLEPFVQAGLLTLDADS
                     LQVTSKGRLFVRAVA"
     gene            complement(1531..2568)
                     /locus_tag="VIR04_05265"
     CDS             complement(1531..2568)
                     /locus_tag="VIR04_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY9460188.1"
                     /translation="MNALVYNGPEKKSWGSVPTPKILKPTDALVKIVKTTICGTDLHI
                     LKGDVPEVRNGRVLGHEGVGIVQEVGAAVRNFKKGDHVLISCITSCGKCEYCKKQMYA
                     HCEDGGWILGHLIDGTQAEYVRIPHADNSLYPIPAGSDEDALVMLSDILPTGFEIGVL
                     YGAVKPGDVVAIVGAGPVGMAALLTAQFFSPGELIMIDADKSRLAMAKKFGATRTINN
                     SKENVAEIIFSETKDGVDVAIEAVGVPQTFDICQTIVRPGGHIANIGVHGKSVDLQLQ
                     TLWIRNITLTTGLVNTNTTPMLLKTVESKRLEPEHLITHRFTFSQFLEAYDIFGNAAK
                     EKALKVIISNS"
     gene            2809..3504
                     /gene="queC"
                     /locus_tag="VIR04_05270"
     CDS             2809..3504
                     /gene="queC"
                     /locus_tag="VIR04_05270"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY9460189.1"
                     /translation="MSHALVLFSGGQDSTACLAWALSRFTVVETVGFDYGQRHIIEME
                     CRQNVLREVRKQFPGWSLQLGEDHVLDLGFLAKISDCALTAKREIAFADNGLPNTFVP
                     GRNLLFLTSAAALAYRRGIDTLVGGMCETDYSGYPDCRDTTMQAMRLALTLGMGQRLQ
                     IETPLMWLDKAATWNLARELGGQALVDITREYSHTCYLGDRSTSHDWGYGCGSCPACK
                     LRARGYAKFISPV"
     gene            complement(3501..>4318)
                     /locus_tag="VIR04_05275"
     CDS             complement(3501..>4318)
                     /locus_tag="VIR04_05275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY9460190.1"
                     /translation="TAGRLAGTSLIVRTRHLANKPHSLLSYTVIPHKVVTVSEYVRQN
                     LIERGVRPDDVATVLSPIQMPPPVRQSTLRAELGLTSSDIVVGCVAVMRAQKGHCDLI
                     DAMEPLIHERSGIHLVFVGGGSPVFEQVQAYVAEKRLQRQVHLMGARSDVPNLMAGFD
                     LFALATRKEASGTVFAEAEAAGLPVVGTDVGGVREMLQDGVSGMLVQLQDTMALTASL
                     RKLIDDPDLRRRMGSAGLRFTRGDGRFSPQATAQCMESCYVRWLNELRVPLPD"
BASE COUNT          995 a         1211 c         1117 g          995 t
ORIGIN      
        1 tggctaccgc ccggacaaac aaacgtcctt tggatgttac ctggaggcta tccgcatcca
       61 gggtgaggag ccccgcttgc acaaagggtt ccagtgtcgc caattcgctg gaaaaataat
      121 cggcaaaatc aacaccgtgc cgttgactga tcagtgcgaa atcgaccggc ttgctgcaca
      181 tgatttccat aattacttgt cggcgcaact gatcctcggc gtttaactcg acaccgcgct
      241 cagtacccag ttgcccatgg tcgagccgat aatagtaggt atgaagattt cgcactgact
      301 ggctgtaaga cgtgcctacc ttgccgatag ccgagacccc cagcccgatc aggtcgcatt
      361 ccgcccgggt tgtataaccc tggaaattcc gatgcaggct attatcaagc cgtgcccgat
      421 tcaattcgtc gtccgatttt gcaaagtggt ccaggccaat atactcgtaa cctgcttcca
      481 gcaagcgttc gaccgacatg agaaatatct gcaaacgctc ctcggtcgac ggcagatcgc
      541 tttgttgaat cagtcgctgg gccttgaagt gacctggcaa gtgggcatag ttgtacaagg
      601 cgattcggtc cggcgataag cggatcaaat tgtccagcgt tcggctgaag gtagccaatg
      661 actgcttcgg caagccgtaa atcaagtcag cgttgatgga cttgaatcca gcggcgcggc
      721 tggcgcggat cgccgcctcg accatctcca gcggctgaat cctgtgaatg gccagttgca
      781 cgtcggggtc gaagtcttgc acgccgaaac tggtgcgatt aaaacccagt ccggccagca
      841 ttgccatcgt cgtatcggtg accgtacgcg gatcaatttc aacgctaagc tcggcatcgt
      901 cggaaaattc gaaatgcgtg cgcaacacgg ccatcagttc atgcagttcg ctggcgctta
      961 agaaagtagg cgtgccaccg ccgaaatgca gttgcaccgt tttgcgatcg gaacccagat
     1021 aggaagcgac catcgccgct tctttaccca gatagcgtac gtactccgca gagcggctat
     1081 ggtcctgggt aatgatttta ttgcaagcgc aaaaatagca aagcgattca caaaacggca
     1141 agtgcacgta caccgatagc ggagggtttt ccagacgttg cgagcgctcg gtcagtttgc
     1201 cgatataggc cgactcggta aattctgcat gaaagcggtc ggctgtggga taggacgtat
     1261 agcgtgggcc tgatacatcg aaacgccgaa ccagttcttc ggaaattgga aggttcggta
     1321 aggatttgcc cggatcgcgg gcgagtaaaa cggcagtatt cacggtttct ccatcaatat
     1381 ccgcgcgacg ttgcatcaag cacctgtccg cggcgtccat tgcgcattac atgtgcagat
     1441 tgttgcattc aatacccaat gtgttcttga taaggatcaa ccaaagccaa tgtctgtgcc
     1501 gactaccgac gtgtctgacg atgcgccgaa ttatgaattg ctgataatga ccttcaaggc
     1561 tttctctttt gccgcgttgc caaaaatatc gtaagcctca aggaattgac tgaacgtgaa
     1621 gcgatgggta atcaagtgct cgggctcaag cctttttgat tccactgttt tgagcagcat
     1681 cggcgtggta ttggtattca ccagccctgt cgttagggtg atattccgga tccaaagcgt
     1741 ttgaagttga aggtccacgc tctttccatg aacgccgatg ttggcaatat gcccacccgg
     1801 ccgcacaata gtttggcaaa tatcaaatgt ttggggcacc ccgaccgctt cgatggccac
     1861 atccactcca tctttggtct cgctgaaaat gatttccgca acgttctctt tggagttatt
     1921 gattgtcctg gtcgcaccaa atttttttgc catggccaga cgagatttat ccgcgtcgat
     1981 cataataagt tcgccaggcg aaaaaaactg agccgtcaat aaagccgcca ttcccactgg
     2041 accagcgccg acaattgcga ccacatctcc cggctttacc gctccataca agacacctat
     2101 ttcaaaaccc gtaggcagga tgtcgctaag cataaccaga gcatcttcgt ccgatccggc
     2161 aggaattgga tacaggctgt tatcagcgtg cggaatgcgg acatattcag cctgcgtgcc
     2221 atcaatcagg tgccccaaaa tccatccgcc gtcttcgcag tgagcataca tttgcttttt
     2281 gcaatactca cattttccgc acgaagtaat gcaagagatc agtacatggt cgcccttttt
     2341 aaaattgcgg actgcggccc cgacttcttg gacaatgccc acgccttcgt ggccaaggac
     2401 ccggccattt cttacctcgg gcacatcgcc ctttaaaata tgcagatcag tgccgcaaat
     2461 cgtagttttg acaattttca ccagtgcatc ggttggtttc aaaattttcg gcgttggaac
     2521 cgaaccccac gattttttct ctggcccgtt atagacgagc gcattcattg tggttgggat
     2581 agacattttt acccccgcat gcgtaatcgg attatcaagc atacccggca gcccgaaacc
     2641 aacatttgat cttgatcaaa cagccacaat ttgcgtagtc ggcaaacttg gaattcgagc
     2701 attttccatt cagtataatg ttgttgcgcg cttatccagc ggcaacactt ccccgttatc
     2761 caacggtccc atgctgcact cacaggcgca tcattcatac caaaaaatat gtcgcacgca
     2821 cttgtattat tttctggcgg gcaggactcg accgcctgtc ttgcctgggc tttaagccgc
     2881 tttaccgtgg ttgaaaccgt cgggttcgat tatggccaac gccatatcat cgagatggag
     2941 tgccgccaaa acgtgctgcg tgaagtacgc aagcaatttc caggatggtc gctgcaactg
     3001 ggcgaagatc acgtgctgga tcttgggttc ctggcaaaga tctcggattg cgcccttact
     3061 gcgaaacgtg aaatagcgtt cgccgacaac ggtctgccca atacgttcgt tcccggaagg
     3121 aacctgcttt ttctgacgag cgctgccgcg cttgcgtatc ggcgcggcat agacacactg
     3181 gtgggcggaa tgtgtgaaac cgattattcc ggttatcccg attgccgcga cacgaccatg
     3241 caggccatgc gcctcgcgct tacgcttggc atggggcagc gactgcagat cgagacgccc
     3301 ttgatgtggc tggataaagc cgccacctgg aaccttgccc gcgaactggg cggccaggcg
     3361 ctggtggaca ttacacgcga atattctcac acgtgttatc ttggcgatcg ctccacttcg
     3421 cacgactggg gatacggttg cggctcctgc ccggcatgta aattgcgggc aaggggttat
     3481 gcgaaattta tttctcccgt ctagtccggc aagggaacgc gcaattcgtt tagccaacgc
     3541 acataacagg attccatgca ctgggctgtc gcctgtgggc taaagcgacc gtccccacga
     3601 gtgaaccgca ggcccgcact gcccatccgg cgacgcaggt ccgggtcgtc gatcagcttt
     3661 ctaagcgagg cggtcaatgc catcgtatcc tgcagttgaa ccagcatgcc gctgaccccg
     3721 tcttgcaaca tttcacgcac gccgcccacg tccgtgccca ccacgggcag gccggcggcc
     3781 tccgcctcgg caaatacggt gcccgatgcc tctttgcgag tcgccagggc gaataaatca
     3841 aagcccgcca tcaagttcgg cacgtctgaa cgcgctccca tcaaatgcac ttgcctttgc
     3901 aaccgctttt cagcgacata cgcttgcacc tgctcaaaaa cgggcgagcc gccgcctacg
     3961 aaaacaagat gaatgccgga acgttcatgg atcaatggct ccatcgcgtc gatcagatcg
     4021 cagtgccctt tctgggcgcg cattaccgcg acgcagccca cgacaatatc actggaggtc
     4081 aggcccagct ccgcgcgcag cgtcgactgc ctgaccggcg gaggcatttg tatcggcgaa
     4141 aggacagtcg ccacatcatc aggacgaacg ccgcgttcaa tgagattctg gcgtacatat
     4201 tcgctgaccg tcaccacctt gtgcggaata acggtatacg acagtaatga gtgtggcttg
     4261 ttggccagat ggcgggtgcg aacgatcaga gaagtgcccg ccagccgtcc ggcagtag
//
LOCUS       DATCXU010000236         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1106547,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000236 DATCXU010000000
VERSION     DATCXU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106547"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VIR04_05280"
     CDS             complement(<1..857)
                     /locus_tag="VIR04_05280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY9460191.1"
                     /translation="MQSPDKSPLEILQQTFGYDSFRGDQQAIIEHVIQGGDALVLMPT
                     GGGKSLCYQIPALVRQGVGVVVSPLIALMQDQVDALIELGVRAAFLNSTQDWRTARDV
                     ERAFLSNELDLLYVAPERLLTERCLDLLSQGVVSLFAIDEAHCVSQWGHDFRPEYLGL
                     SVLAERWPAVPRLALTATATAVTRREIAQRLELVDAPCFVASFDRPNIRYRIVEKNEV
                     RKQLLSFIQTDHPGDCGIVYCLSRARVEETADFLCSHGILALPYHAGLSASVRAANQS
                     RFLREDGLV"
     gene            complement(896..1237)
                     /locus_tag="VIR04_05285"
     CDS             complement(896..1237)
                     /locus_tag="VIR04_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY9460192.1"
                     /translation="MDALAENPVTHQRRSAGLQAERTALAWNRTGLAVLANALLVLRA
                     GSQSHQASLVVVASALLVAAGGAVFYGAWRRRHLLSGQGTIAPRSAAIVTTAIVVWVA
                     CAAGIASILLR"
     gene            complement(1237..1587)
                     /locus_tag="VIR04_05290"
     CDS             complement(1237..1587)
                     /locus_tag="VIR04_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY9460193.1"
                     /translation="MHEPRWRQEGEDPDYRFSLANERTFLAWIRTALALLAGGVLLDQ
                     FSTKLEPHAVVVILAVVLSLLAAVFCAIAYLRWRANEIAMRHSRKLPGTIGIPLVAGA
                     ILGIAAVIALLLIL"
     gene            1759..2460
                     /locus_tag="VIR04_05295"
     CDS             1759..2460
                     /locus_tag="VIR04_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY9460194.1"
                     /translation="MDKPLVKVLVVDDDPALRQLLADYLNRHGYDTLLAPDASDLAAR
                     IQRYSPDLIVLDRMMPGGDGAQACRRLRELGEDIPLILLTARDETVDRVIGLESGADD
                     YVGKPFDPRELLARIEAVLRRKKGASALNKEKPIAFGPFVFDPATRQLLKNQTPIKLT
                     GGEVNLLEALVNNPGKPLSRDRLLALARDDDEGERNDRAIDIAVLRLRRSIEDDPKQP
                     RWIQTVWGVGYRFAP"
     gene            2464..>2793
                     /locus_tag="VIR04_05300"
     CDS             2464..>2793
                     /locus_tag="VIR04_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY9460195.1"
                     /translation="MLGSLVPRSLRTHLILSTLGIILLVQAATVATVSYYRKQYTEQV
                     AVHFTATTIRTLRASLAEIPTDQRADFVRQASQNEWHLWSRNLPAEANVQERRLRPRR
                     GRSAQTPP"
BASE COUNT          641 a          876 c          761 g          515 t
ORIGIN      
        1 ataaccagcc cgtcttcacg caaaaaccgg gactggtttg ccgcccggac cgatgcgctc
       61 aagccggcgt gatacggcaa cgcgaggatt ccgtgactgc ataaaaaatc agcggtctct
      121 tcgacccttg cgcgcgacag acaataaaca atgccgcaat cgcctgggtg atcggtctgg
      181 atgaagctta acaactgctt tcgcacttcg tttttttcaa cgatgcgata gcgaatattc
      241 ggccgatcaa agctcgccac aaagcaaggt gcgtcaacaa gctccagacg ctgcgcaatt
      301 tcacgccggg tgaccgccgt cgccgtcgcc gtcaatgcca aacgcggcac agcaggccag
      361 cgttcggcaa gtacggacaa acccagatac tcggggcgga aatcatgtcc ccattgcgac
      421 acgcagtgcg cctcatcaat ggcaaaaaga gacacgacgc cttgcgacag caaatccagg
      481 catcgctcgg tcaacaaacg ctcgggcgcc acatacagca aatcgagttc attggacaaa
      541 aacgcacgct cgacgtcgcg cgccgtgcgc caatcctgcg tcgaattcag gaacgcggcg
      601 cgcacgccaa gctcgattag cgcatctacc tgatcttgca tcagggcaat aagcggcgat
      661 acgaccacac ccacgccttg ccttacaagc gcgggaatct gataacacaa ggacttgccg
      721 cctcctgtcg gcatcagaac cagcgcatcg ccgccctgga tgacatgttc gataatggcc
      781 tgctgatcgc cacgaaagga atcgtagccg aatgtttgtt gtaatatttc cagcgggctt
      841 ttatcgggcg attgcattca acactataga aagacggatt gatgaccgtg tcagtttaac
      901 gcagcagaat cgatgcgatg ccggctgcgc atgcaaccca gacgacaatg gccgtcgtca
      961 cgattgccgc cgagcgcgga gcgatcgtcc cttggccgct taataaatgc cgccggcgcc
     1021 acgccccata aaaaacggcc cctcccgccg caaccaacaa cgccgaggct acgactacaa
     1081 gggaagcttg atggctctgc gagcccgcgc gcagcaccaa cagtgcgttc gccaaaaccg
     1141 cgaggcccgt gcgattccag gccagtgcgg tgcgttcagc ctgcagtccg gcgcttcgac
     1201 gctggtgcgt tacaggattt tcggcaagcg catccatcac aaaatcagca atagcgcaat
     1261 gaccgcggcg atacccaaaa tggcgccggc aaccagcgga ataccgatag tacccggcaa
     1321 tttgcggctg tggcgcatcg cgatctcgtt tgcgcgccag cgcagatagg caatcgcgca
     1381 aaatactgca gccagcaggc taagaacaac agcaaggatc acgacaacag cgtgcggctc
     1441 caatttagtc gaaaactggt cgagcaagac tccacccgcc aggagtgcaa gagctgtgcg
     1501 tatccaggca agaaaggtgc gctcgttggc cagcgaaaat cggtagtccg gatcttcgcc
     1561 ttcctggcgc catcgcggct cgtgcattca gtatccctct ctggttcttg gccgcgttgc
     1621 gcgcgccgtc gcagacgtag tatcgccgcg cgacttttga tcgtagcata tgcaaaccca
     1681 attaaaaaaa ttgggacagg tatttcagtc aatttcgaac agcttgccag ccgacgcgtg
     1741 aaccggcaca ctggaatcat ggacaaaccc ctcgtaaaag tactggtcgt cgatgacgat
     1801 cccgctctgc gtcagttgct ggccgattat cttaaccgtc acggttacga caccctgctt
     1861 gcgcccgacg ccagcgatct ggcggcgcgc attcagcgct acagccccga ccttatcgtg
     1921 ctggaccgga tgatgcccgg cggcgatggc gctcaagcct gccggcggct gcgggaactg
     1981 ggcgaagaca ttccccttat cctgctgacc gcgcgcgacg aaaccgtgga ccgggtcatc
     2041 ggtctggaat cgggtgcgga cgactatgta ggcaagccct tcgacccgcg cgaactgctg
     2101 gcccggatcg aagccgtcct gcgccgcaaa aaaggggcgt ccgctctgaa taaggaaaag
     2161 cccattgcct ttggtccgtt cgtgttcgac cccgccaccc gtcagttgct caagaaccag
     2221 acgcccatca aacttaccgg cggcgaagtc aacttgctcg aggccctggt caataacccg
     2281 ggcaagcctt tatcgcgtga tcgcttgctg gcgctggcgc gcgacgatga cgaaggtgag
     2341 cgcaacgatc gcgccataga catcgccgtt ttacgtttgc gccgatccat cgaagacgat
     2401 cccaagcagc cgcgctggat acaaaccgtc tggggcgtgg gctaccgctt tgcaccataa
     2461 cgcatgctag gttcgctcgt tccgcgctct ttgcgcacac atctgattct gtcaacttta
     2521 ggcattatct tgctggtgca ggcggcgacc gtggcaacgg tatcctatta ccgcaaacag
     2581 tacacggaac aagtcgcggt gcactttacc gccacgacga tacgtaccct gcgcgcctcg
     2641 ctggccgaaa ttccaacgga ccaacgggcc gacttcgtgc gccaggcatc gcaaaatgag
     2701 tggcatttat ggtcgcgcaa tctgcccgcc gaagccaacg ttcaggaacg ccggcttcgt
     2761 cccaggcggg gccgcagcgc gcagacccct cct
//
LOCUS       DATCXU010000237         4346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_245789,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000237 DATCXU010000000
VERSION     DATCXU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245789"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VIR04_05305"
     CDS             <1..330
                     /locus_tag="VIR04_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460196.1"
                     /translation="AGISSRRVYTWAWGLVGASGSVAGVCIAWRSSLYPDLGWDALLV
                     AFAAAILGGIGNIRGAVIGGFLMGIASSMATVFVAPTFKPVFGFIAMALILLMRPQGL
                     LGKRERI"
     gene            327..1304
                     /locus_tag="VIR04_05310"
     CDS             327..1304
                     /locus_tag="VIR04_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460197.1"
                     /translation="MTTYLPAILSITFVYGILGLALNLNWGRTGIINFGVVAFFAIGA
                     YTSALLNTEFNVPIWAGCILGAILAGLAAVPVGWVTLRLKADFLAVVTIGFAESLRIF
                     VQSVDWTGGPGGVIGIERPFSDYSSDTFNMAWFFIFLTGIIFSYLIVHAITKSRLGSL
                     LRAIKYDEAATSMLGKNVTAAKTLSLVITAMITGVTGALYAHFIGYISPDQFTALVTF
                     YVWAGIIIGGSSDLGALVGSMVLVGLLESTRFLDVLGFGVASAANMANFRMIAVGLVI
                     LLFLKYRPDGLFPYDAAKNTRRPIRAPNDDVPVVADGKVSKGTTLHVEN"
     gene            1291..2052
                     /locus_tag="VIR04_05315"
     CDS             1291..2052
                     /locus_tag="VIR04_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460198.1"
                     /translation="MLRINGLAVSFGSVSVLKNISFECKPGQVTAIIGPNGAGKTTLF
                     DAMTGFTPTLAGSTECGKTNISGLAPFLVARAGLSRTFQTPRLFDRMTVLENLKVAHE
                     DEGLFELAMSALTRSGELRQDRTASSKAIELLRSLQLEHVKDNFAADLSGGQRKLVEL
                     GRALMTDPQYILLDEPVAGVAPALVEDIGRRLRELAQGGLGVVLIEHNMDFVMKVSDY
                     VVVMTNGSVLTSGAADEVRADPRVLEAYLSGATEL"
     gene            2052..2759
                     /locus_tag="VIR04_05320"
     CDS             2052..2759
                     /locus_tag="VIR04_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460199.1"
                     /translation="MPLQVSNIIAGYGRVMILDSVCIHLGNNEIICIAGPNGAGKSTL
                     LKAIAGIVPWQSGQVSLFGAAAVSGETSDEARGVLGVVPQVSNVFPSLSVHENLLVVL
                     PSHWDKHKKKSALERVYGMFPDLYEIRGKSSANLSGGQRQSVAFAMALVREPKMLLLD
                     EPTAALAPIAAQAVFKRIVGLKEAGIPILLVEQNVRAAMEIADRAYFLENGRNFLDGE
                     VSVLRDDPRIRKIYLGA"
     gene            2828..3817
                     /locus_tag="VIR04_05325"
     CDS             2828..3817
                     /locus_tag="VIR04_05325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY9460200.1"
                     /translation="MLTAEMDVADMVAVIGPSVGLSVVVKQKRLLAERIIGLTLESSE
                     DADLPPWEPGAHIDLIVPGGAIRQYSLYGNRRNPRQYQIGVLLEPDGAASSFIDKDVK
                     EGDTIRIGKPRNHFGLAQFHRYIFIAGGIGITPFLPMLEFLEEQHKQWMLYYAGRNQS
                     SMAFFDNLSKFGNKVVFAPKAEGKRLDLKSILGAAQSDTAVYCCGPNRMIDAVSDTCL
                     GVWPKGAIYAERFAAVEQTAGENQPFKIRLERSNLTLDVPADRSAIDVMADAGVFIPC
                     SCKEGTCGSCEVDVLEGVPDHRDSVLTEEERDSNSIMMVCISRSKTPLLVLDA"
     gene            3888..>4346
                     /locus_tag="VIR04_05330"
     CDS             3888..>4346
                     /locus_tag="VIR04_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY9460201.1"
                     /translation="MKTISASKLLEDQHHEIDRGILGVLYGGEKITSHADKISLLRVH
                     IYIEEEILFPMLEKAGVMLPITEMNREHAQMWPYIEALTAACGDKGSNETAWDSCVSL
                     YNILQAHNHKEEDIIYSVVDQLLEGTAGKALVTAIEAATIPDGWVCTMARS"
BASE COUNT          947 a         1015 c         1274 g         1110 t
ORIGIN      
        1 gcgggaattt cctcccgcag ggtctatacg tgggcctggg gactggtcgg tgcttccggg
       61 tcagtcgcgg gcgtatgtat tgcctggcga tcatctttgt atccggatct tggatgggac
      121 gcgttgctgg tggcttttgc ggcggcaatt ctaggtggaa ttggcaatat tcggggagcc
      181 gtcatcggtg gtttcctgat gggcattgcc agcagcatgg cgacagtatt cgtggcgcca
      241 actttcaagc ctgtcttcgg gttcatagca atggccctga tccttttaat gcggccacaa
      301 ggcttgttgg gaaagaggga acggatatga cgacctattt gcctgcgatc ctgtcaataa
      361 cctttgtata cggaattctc ggccttgccc tgaatttgaa ttgggggcgc actggcatca
      421 taaattttgg ggtggttgct ttttttgcaa ttggcgccta tacgtcggcg ctattgaata
      481 cagaatttaa cgttccgatc tgggcgggat gcattttggg ggcaatcttg gcaggattgg
      541 cagcggtgcc agtagggtgg gtcacactca ggctgaaggc ggattttctg gctgtggtca
      601 ctattggttt tgcggaatca ctgcgtatct tcgtccagag cgttgactgg acgggtggcc
      661 ccggtggtgt tatcggaata gagcggccct tttcagatta ttcgtccgac actttcaaca
      721 tggcctggtt tttcatcttt ttaactggca tcatcttttc ttatcttatt gtccatgcca
      781 taacaaaaag ccgtctcgga agtctgttgc gggcaattaa gtatgatgaa gcggcaacgt
      841 ccatgcttgg caaaaatgtc acggcggcca agaccctgag cctggtaatc acagccatga
      901 ttactggcgt cacgggcgct ttatatgcac atttcattgg ctacatatcg cccgaccagt
      961 ttacggcact cgtaacgttc tacgtttggg cgggcatcat catcggaggc tcgagcgact
     1021 tgggcgcgct agtgggcagc atggtgcttg tcggcctgct tgaatcaact cgtttcctgg
     1081 acgtactcgg ttttggcgtg gcctcggctg caaatatggc caatttccga atgatcgcag
     1141 tcggccttgt catactgctg tttctaaaat acaggccgga cggtttattc ccatacgatg
     1201 cggcaaaaaa cacaagacga ccgatcaggg caccgaacga tgatgtgcct gtcgtggcag
     1261 atggcaaagt cagcaaagga accacgctcc atgttgagaa ttaatggtct tgccgtatcc
     1321 tttggcagcg tatctgtact taaaaacatt agctttgaat gcaagccggg gcaagtgaca
     1381 gccataatcg ggccaaatgg tgcgggcaag acgacgcttt tcgacgcgat gaccggcttc
     1441 actcccaccc tggcgggatc aactgaatgc ggcaaaacga atatttcagg tttggcgccc
     1501 tttctggttg cgcgtgcggg tctttctcga acgttccaga cccccagact gttcgatcgc
     1561 atgaccgtgc tggaaaattt gaaggttgcg catgaggacg agggcctgtt tgaacttgcc
     1621 atgtcggcat tgacgcgatc gggagagctc cgccaagatc gcacggccag cagcaaggca
     1681 atcgaactgc taaggtcttt gcagcttgag catgtaaagg ataattttgc tgctgatttg
     1741 tccggcgggc aaaggaaact agtggagctt ggccgagcgc tcatgacgga tccgcaatac
     1801 atacttttgg acgagccggt cgcgggtgtc gcgcccgctc tggtcgaaga tatcggcaga
     1861 cggttgcgtg aactggctca ggggggactg ggggttgtcc tgatagaaca caatatggac
     1921 tttgtcatga aggtcagtga ctatgtcgtg gtgatgacca atggttcggt cctgaccagt
     1981 ggcgcggcgg acgaagtaag ggccgatccc cgagtgctcg aggcttattt gagcggggca
     2041 acggaattat gatgccgctt caggtcagca atatcatcgc agggtatggc cgggtgatga
     2101 ttctggattc ggtctgtatc catcttggca acaatgaaat aatctgtatt gccgggccga
     2161 acggtgcggg caagtcgaca cttctgaagg cgattgcggg catcgttccg tggcaaagtg
     2221 ggcaggtctc cctcttcggc gcggcggctg tcagcggtga aacatccgat gaagcgcgcg
     2281 gcgtccttgg tgtggtgccg caggtcagca atgtattccc ttccctttcc gtacacgaga
     2341 accttttagt cgttttgccc tcgcattggg ataaacacaa gaagaaatca gccctggagc
     2401 gcgtctatgg catgtttccc gacctttacg agattcgcgg taaatcgagt gcgaatctgt
     2461 ccggaggcca gcggcagtcg gttgcgttcg cgatggcact ggtgcgtgaa ccaaaaatgc
     2521 ttctgcttga cgagcccacc gcagccctgg cccctattgc cgcgcaggct gtctttaagc
     2581 gtattgtggg gctgaaagaa gcaggcatcc cgattttgct tgtagagcaa aatgtgaggg
     2641 ccgccatgga gatcgccgac agggcgtatt tcctggaaaa cggacggaac tttcttgacg
     2701 gagaggtttc tgtattgcgt gacgatccgc gaattcgtaa gatctacctt ggagcgtaag
     2761 gcttcatttg ttgccgcgcg cccgtgaaga agaattgttg tgtctttagt tgttttttgg
     2821 aaatgctatg ttgacggctg aaatggatgt tgctgatatg gtcgcagtca tcggtccgag
     2881 cgtgggtttg tccgttgttg tgaaacagaa gcggttgctc gctgagcgaa tcatcgggct
     2941 cacactggaa tcaagtgaag acgcagatct tccgccctgg gagccgggcg cccatatcga
     3001 cctgattgtt cccggcggcg caatacggca gtactcgcta tatgggaaca ggcgcaatcc
     3061 ccgtcaatac cagatcggag tgcttctcga gccagacggc gcggcgtcgt cctttattga
     3121 caaggatgtg aaggaagggg acaccattcg tatcggcaag cccagaaatc attttggcct
     3181 cgcgcagttt cacagatata tttttattgc tggcgggatc ggaattacgc cgtttctgcc
     3241 gatgctggag tttctcgaag agcaacataa gcagtggatg ctttattacg caggtagaaa
     3301 tcagtcctca atggcttttt ttgataatct gtccaaattc gggaacaaag ttgtctttgc
     3361 gcccaaggca gaggggaaac gccttgattt gaaaagcatt cttggcgcgg cacaatccga
     3421 taccgcagtc tattgctgtg ggccgaatcg tatgatcgac gctgtttcag atacttgtct
     3481 gggtgtgtgg ccgaagggtg caatttacgc tgaacgcttc gccgctgtcg agcaaacagc
     3541 cggtgaaaat cagccattca agattcggct cgaacgtagc aaccttactc tcgacgtgcc
     3601 tgcggacagg tcggcgatcg acgtaatggc cgacgccggt gtatttattc catgttcctg
     3661 caaagagggc acgtgcggct cctgtgaagt tgatgtcctg gaaggtgtgc cggatcacag
     3721 agattcggtg cttaccgagg aagagcgcga ttcgaactcg atcatgatgg tttgcatatc
     3781 gcgctccaag acgcctttat tggtgcttga cgcttgatgg cctttgtttt tatgcatcaa
     3841 tgttttcatg cggttggctg aaattaactt aaatgagaga ttgaacgatg aagacaatat
     3901 cggcgagcaa gttacttgag gatcagcatc acgaaatcga tcggggaatc cttggggtcc
     3961 tttacggagg agaaaaaatt acctcgcatg cagacaagat atcgttgttg cgtgtccaca
     4021 tttatatcga ggaagagatc ctctttccga tgctggagaa ggcgggagtc atgttgccga
     4081 tcaccgagat gaatcgggaa cacgcgcaga tgtggcctta tatcgaggca ttgaccgctg
     4141 catgcgggga taagggttcg aacgaaacgg cttgggattc ctgtgtgagc ctctataaca
     4201 ttttgcaggc ccacaaccat aaggaagaag acattattta ttctgtcgtt gatcagttgc
     4261 ttgaaggaac ggctggcaag gcgctggtca cggcaattga ggctgcaacc attccggatg
     4321 gctgggtttg caccatggcc aggtca
//
LOCUS       DATCXU010000238         9402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_316239,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000238 DATCXU010000000
VERSION     DATCXU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316239"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..618)
                     /locus_tag="VIR04_05335"
     CDS             complement(91..618)
                     /locus_tag="VIR04_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY9460202.1"
                     /translation="MSFESDVVVLLPILRRYAHALTGNAAWADDLVQDTAERALRRWT
                     TWSANGNLRAWLLTILRNLYIDQLRKQREIATGDNDALWRGMAVPEEKVDGLFLRDVQ
                     RALYSLPMEQREVLLLIGLEELSYQEASSILKIPLGTVMSRLSRAREHMHGLMFAKKA
                     NLRGKPALKVVRGTQ"
     gene            complement(838..1203)
                     /locus_tag="VIR04_05340"
     CDS             complement(838..1203)
                     /locus_tag="VIR04_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460203.1"
                     /translation="MHKTKALFLSLICAGIAAPALAEMPKMSNGHWVDEQGATLYTFD
                     KDSKGKSACDAACAANWPPAAADSYDKASGDWSVIKRSDGTNQWAYKGHPLYRFAKDK
                     KPGDAMGDGVKGMWHVSKS"
     gene            1377..3029
                     /locus_tag="VIR04_05345"
     CDS             1377..3029
                     /locus_tag="VIR04_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEY9460204.1"
                     /translation="MNRVLANRFYYLENFETVLHWIEQRHNDLLAQPERDFITRFLML
                     PRPSRALLVRMIMRKGTLFRSGKLRYDEVGDSTGAALTLVDQGWLDDHPAVTIEELFS
                     LLTKAEISNIFAASLPNKNLKKTEQLIILGAQSTEARQFGDWWDGAAEPVYRLLIMPL
                     CERFRLMFFGNLRQDWSEFVLADLGLYAYEKVDFAPDSRVFRTRRDIDDYLHLHDCRE
                     RFRAGASPELVMEELPSLPYQNEWLEQRRAKLLFQIAQHYEKAGDWLNALDAYSSCGH
                     IGARLRTIRVLERSGQFEPALALARIAWRQPESETESQRLNRIVPRLERRLIGHIAPR
                     ERMAPIARLTIALPKPPTVVAVEELAREHLQRQSHGTVHYVENALVNALFGLLCWESI
                     FAAIPGAFFHPFQRGPADLYSADFYQRRKTRFSACLAQLESGQYKQTIFRNFKEKAGI
                     RSTFVAWEVISEEILKQALSCIPATHLKKCFERMLLDVASNRSGFPDLIQFWPFEKRY
                     RMIEVKGPGDRLQDNQLRWLDYCAVNDMPAVVCHVQWAQAAS"
     gene            3026..5338
                     /locus_tag="VIR04_05350"
     CDS             3026..5338
                     /locus_tag="VIR04_05350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY9460205.1"
                     /translation="MKYAVAVRTLCEFTAKQGDLDVRFTPSPTALEGMAGHVAVTSRR
                     AAGYQAEITLAGEFEQLAVRGRADGYDPRQNQVEEIKTFRGDLNSMPANQRHLHWAQV
                     RIYGHLLCQKLGLSEIKLALVYFDLASKRETVLSESVRADSLKQNFEDQCKLFLTWAE
                     QELAHRAGRDRALGALQFPHAAFRTGQRPLAEAVYRAARAGRCLAVQAPTGVGKTMGA
                     VFPILKAMPTQALDKVFFLTAKTSGRAVALNALTHIINSAPEMPLRILELIARDKACE
                     YPDKACHGESCPLAKGFYDRLPGARRAALALGRMDRLALRQVALEHQVCPYYLSQDLV
                     RWCDVVVCDYNYYFDLNAMLYGMTVVNQWRVAVLVDEAHNLLERARKMYSAELDQERF
                     KSVRRNVAPELKKSFDSVNRSWNELQKNQTASYQSYDGVPTKFTGALQRVTGCINDYL
                     AENPAQTDSRLQRFYFDALHFLHMADLFGNHSVFDITLTSTRNLAARASSIISLRNII
                     PASFLAPRFVLTHSTILFSATLTPWRFYSDTLGLPTDAAWLDVEPPFKTGQLAVQVVS
                     AISTRYRDRSQSLSPITDVMGRQFFKEPGNYLAFASSFDYLRQLADLFETRYPHIPIW
                     RQSRGMDETQRTTFLNRFTETGQGIGFAVLGGAFAEGVDLPGRRLIGAFIATLGLPEI
                     SPANQRFMQRMDSAFGGGYEYTYLYPGIRKVVQAAGRVIRGETDRGVVYLMDDRFGRR
                     DVQRLLPKWWHVCAFPAGGHDTCSPLTTTR"
     gene            5364..6521
                     /locus_tag="VIR04_05355"
     CDS             5364..6521
                     /locus_tag="VIR04_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEY9460206.1"
                     /translation="MLRSLNSLNARTDGPRIAVAFPRRISAPPHEVASHCHLTQRLAI
                     LLGRDFIVDYDPLVRNTVDNIYYVPYRTLVEPGAAGSTEEQLMNLSGLHDLFGGVVPY
                     PFVGTKAITHSLVDRDARAPIGWSKKFGEHVRQAVLQGATVFSPEDARRAGAVLIRAG
                     PVRIKPVLGIGGRGQNVVESQSALNDAIEEQNATELNDCGLVLEQHLDDVCTYSVGHV
                     SVGDLVVTYVGTQSLTRDNAGEMVYGGSDLLFARGEFEALLAINLTDAEREAVRLARI
                     YDAAALRCYPGLYASRRNYDVAQGKDIWGRVRTGVLEQSWRAGGASVAEACALEAFHR
                     APDLRTLRAFTCERYGAQHPQPAPAQLVYRGQDTEIGFITKYGGISSYGDK"
     gene            6508..7263
                     /locus_tag="VIR04_05360"
     CDS             6508..7263
                     /locus_tag="VIR04_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9460207.1"
                     /translation="MATNSETTHITVQDQRIAGTFLVPKNKVPGVLFIHGWGGSQQFD
                     LLRAKSIAGLGCICLTFDLRGHADTQAQQMKVSRDDNLHDVVAAYDCLIAHPSIDTSA
                     VVVVGSSYGGYLAALLTGLRQVQWLALHVPALYRDDEWMLPKRQLNREILTAYRLSHV
                     GPEDNRALAACRNFRGDVLIVEAEHDNFVPHSTIMNYRSAFQHSHSLTHRIVDQADHA
                     LTNKAAQQAYTTILSNWTTEMVVGARVGSLRPT"
     gene            7348..8115
                     /locus_tag="VIR04_05365"
     CDS             7348..8115
                     /locus_tag="VIR04_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY9460208.1"
                     /translation="MPLSKLIQRLTLKVLTINAHKGFTIFNRRFILRELRDAVRQAAP
                     DIVCLQEILGEEQRRTARIKESPVLSQYEFLADTLWPEFAYGRNAVYPRGHHGNAVLS
                     KYPISTYKNYDVSISPHEGRGLLHCILNPVDMGLNVHVICVHLGLRESHRRHQLRRLC
                     DLVSSTLPNDEPLIIAGDFNDWRQRAEGLLQRCGVTEVFASSLGRPVRTFPARCPLLR
                     LDRIYVRNVKASRPIMMAARPWSRLSDHIALSAEIQL"
     gene            8112..9326
                     /gene="clsB"
                     /locus_tag="VIR04_05370"
     CDS             8112..9326
                     /gene="clsB"
                     /locus_tag="VIR04_05370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEY9460209.1"
                     /translation="MTTPWRGGNEFMLLENGEHYFPRVTQAIGLAKTEILLETFILFD
                     DKVGRALRQALIDAARRGVEIDMTVDGYGSDTLSDEFISGMTDCGVRFHVFDPRPRLL
                     GMRTNLFRRMHRKIVSIDGQLAFVGGINFSADHLPDFGPEAKQDYSIEIRGPLALDIR
                     NLAREALDVKQAGRRRTRICRRPKEAHHSGEAQGRLVVRDNGEHRTDIERSYRAGIRA
                     AKNDILIANAYFFPGYRLLRDLAKAARRGVRVRLILQGQPDMLIARLAARMLYEYLAD
                     AGVRVYEYCQRPLHGKVACIDTTWSTVGSSNLDPFSLALNLEANVFIQDSVFNATLRE
                     RLEDLLACHCRPAPASISSGGHLRRLWVGVVVFHFLRRFPAWAGSLPAHKPELHSIAG
                     RGYADTDTKEAT"
BASE COUNT         1958 a         2635 c         2660 g         2149 t
ORIGIN      
        1 cgcgtggtga aagctcatta tcaaccagcg ccgagagggc ctccaggtgt gctccatcca
       61 gatttgatcc tttcgaagtc gaaccgtcga tcattgcgtg cctctcacca ccttaagcgc
      121 tggcttgccg cgcaggttcg cttttttggc aaacatcaag ccatgcatgt gttcgcgcgc
      181 acgggagagt cgggacataa cggtgcctag cggtattttc agtatgcttg aggcctcctg
      241 gtagctcagc tcttcgagcc cgatcagcag caatacttcg cgttgctcca tgggtaagct
      301 gtatagcgcg cgctgcacat cacgcagaaa aagcccatcg actttttcct ccggaacggc
      361 cataccgcgc cagagggcgt cattgtcgcc ggtcgcgatc tcgcgctgct tgcgcaactg
      421 gtcaatgtac aaattccgca gaatagtcaa caaccaggcg cgcaagttgc cgttcgcgct
      481 ccaagtggtc catcggcgca gggcgcgttc ggcagtgtcc tggacgaggt cgtccgccca
      541 agccgcgttc cccgttagcg cgtgcgcata tctccgaaga atcggtaata aaacgaccac
      601 atcggactca aagctcatgg cgcgccacag acgcgttgca atcgcgttag taccctcccc
      661 cgccgccacc gccaccgcca tcgccagtgg tgccactggt ggacggattg ctgccccgcg
      721 atccgtcgct ggtcatgcat cccgaaagaa taagtgtgat cgccactaaa caagccgaaa
      781 gaattctagg taagctcata acagtctcct gtgaaggcgg ttgacgcgcc tcgcggatta
      841 ggattttgaa acatgccaca tgcctttgac tccgtcgccc atcgcatcgc cgggcttttt
      901 atccttcgca aacctgtata aagggtgtcc tttgtatgcc cattggtttg ttccatcgct
      961 tcgtttgatc acgctccagt ctccgctggc cttgtcatat gagtcggccg ccgccggtgg
     1021 ccaattcgcc gcgcatgccg cgtcgcatgc gcttttacct ttactgtcct tgtcaaaagt
     1081 gtagagcgtt gcgccctgtt catcgaccca gtgaccattg ctcattttag gcatttcggc
     1141 aagagcgggt gcggcaatgc ccgcacatat caacgataaa aataatgctt ttgtcttatg
     1201 catgacatct cctttatgat tgaatggatg gcaaccctga acgcgacgtg tcgtcccggc
     1261 aagggcggcg attccagcac ataaacgttt ggcgccggct gtttattcca tggcatgcga
     1321 tagttttgct gaattatttg atatactgtt tttaaagaca gtgtttcgcg ccgaaaatga
     1381 atcgagtcct cgctaaccgt ttttactacc tcgaaaactt cgagacggtt ttgcattgga
     1441 tcgagcagcg ccataacgat ctgctcgccc agcccgagcg tgatttcata acccgcttcc
     1501 ttatgttgcc ccggccgtcg cgggcgctcc tggtgcgcat gatcatgcgc aaaggaacgc
     1561 ttttcagaag cggcaagctg cgctacgacg aagttggcga ctccaccggc gccgcactga
     1621 cgctggttga tcagggctgg cttgacgacc atccggccgt cactattgaa gagttgttca
     1681 gcttgctgac gaaggccgaa ataagcaata tttttgctgc gtcgttgcca aacaagaacc
     1741 tgaaaaagac tgaacaactt attatccttg gcgctcaatc cactgaagcc cgccagtttg
     1801 gcgattggtg ggacggtgcg gccgagcccg tttaccgatt gctgatcatg ccgctgtgcg
     1861 agcggtttcg actcatgttt tttggcaatt tgcgtcagga ctggtccgag ttcgtgctgg
     1921 ctgatctcgg cttatacgcc tacgaaaagg tcgactttgc gcctgattcc cgggtgttcc
     1981 ggacacggcg cgatatcgac gattatttgc atttgcatga ttgccgcgag cggtttcgcg
     2041 cgggcgcctc gcccgaactt gtaatggaag aattgccttc gctgccttat cagaatgaat
     2101 ggctcgagca acgccgcgcc aaattgctgt ttcagatcgc gcaacattac gaaaaggccg
     2161 gggattggct caatgccctc gatgcgtact caagttgcgg gcatattggc gcacggctcc
     2221 gcaccatacg ggttttagag cgtagcgggc agttcgaacc ggccctggcg ctggcccgca
     2281 tcgcgtggcg gcaaccagaa agcgaaacgg aaagtcagcg gctgaaccgg atcgttccaa
     2341 gactggagcg caggcttatc ggccacattg cgccgcgcga gcgcatggcg cccatagcca
     2401 ggctaaccat tgccttgcca aagcctccaa cggtcgttgc ggtcgaggag cttgcgcgcg
     2461 agcacttgca gcggcaatcg cacggaaccg ttcactatgt cgaaaatgcg ctggtcaacg
     2521 cgctgtttgg cctgttgtgc tgggaatcca tcttcgcggc cataccggga gcgttttttc
     2581 atcctttcca gcgtggccct gctgacctct acagcgccga tttttatcag cgtcggaaaa
     2641 cgcgcttcag tgcgtgtctg gcccagcttg aatccgggca atacaaacag accatctttc
     2701 gaaacttcaa agagaaagcg ggaattcggt cgactttcgt ggcatgggag gtcatcagcg
     2761 aagagatatt gaagcaggcg ttaagctgca ttcccgccac acatctaaaa aaatgcttcg
     2821 agcgaatgct gttggacgtt gcgtccaatc gatctggatt tcccgatctg atccagtttt
     2881 ggccgtttga aaaacgttac cggatgatcg aagtcaaggg ccctggcgac cgtttgcaag
     2941 acaaccaatt acgctggctt gattactgcg ccgtgaacga catgccggcg gtggtctgcc
     3001 atgttcaatg ggcgcaggcc gcatcatgaa atatgcggtt gctgtgcgca ccttgtgcga
     3061 attcaccgcc aaacagggcg atcttgatgt gcgtttcacg ccgtccccca ccgctttgga
     3121 aggcatggcc gggcatgtcg cggtcacgtc gcggcgagcc gctggttatc aagccgaaat
     3181 cactcttgcg ggcgaattcg aacaacttgc tgtgcggggt cgggccgatg gttatgatcc
     3241 ccgtcagaat caggtcgaag aaattaaaac ctttcgcggc gacctgaact ccatgccggc
     3301 caaccagcgt catttgcatt gggcgcaggt cagaatctac ggccacttgc tttgccaaaa
     3361 actgggcctg tccgaaatca agctggcgct ggtctatttt gaccttgcca gcaagcgcga
     3421 aaccgtgctt agtgaatctg tgcgcgccga ttcacttaag caaaacttcg aagaccagtg
     3481 caagctcttt ttgacttggg ccgaacaaga actggcgcac cgcgccggtc gcgatcgcgc
     3541 gcttggcgcg ttgcaatttc cacatgcggc gtttcgtacg ggccagaggc ccctggccga
     3601 ggcggtctac agggccgccc gcgctggccg ctgcctggcc gtgcaggcgc ccacgggcgt
     3661 cgggaagacc atgggtgccg tgttcccaat tttaaaggcg atgccgaccc aggcactgga
     3721 taaggttttc tttttaactg caaaaacttc cgggcgagct gtggcgttga atgcgttgac
     3781 gcatatcatc aatagcgcgc ccgaaatgcc actgcgaatc ctggaactca tagccaggga
     3841 taaagcatgc gaataccccg acaaggcgtg ccatggcgaa tcatgcccgc tggccaaagg
     3901 attctacgat cgcctgccag gcgcgcgtcg ggcggcactc gcgctcggac gcatggacag
     3961 gctggcgttg cgtcaggttg ctttggagca tcaggtatgc ccttattatt taagccagga
     4021 tctggtgcgc tggtgcgatg tggtggtctg cgactacaac tattactttg atttgaatgc
     4081 catgctgtat ggtatgactg tcgtcaacca atggcgcgtc gccgtgctgg ttgacgaggc
     4141 acacaatttg cttgagcgcg cgcgcaaaat gtatagcgcc gaattggatc aggagcgctt
     4201 caaaagtgtg cgtcggaatg tggcgcccga gctgaaaaaa tcgtttgaca gcgtcaatcg
     4261 cagttggaat gaactgcaaa aaaatcaaac ggcaagctac caaagctatg acggcgtccc
     4321 gacgaaattc acgggcgcat tgcaacgcgt aaccggatgc attaacgatt acctggccga
     4381 aaaccccgcg caaaccgata gccggctgca acgattttac tttgacgcgc tgcatttcct
     4441 gcatatggcc gatctgttcg gcaaccattc agtattcgac attaccctta cttccacgcg
     4501 taatttggcc gctcgcgcaa gttcgattat aagtcttcga aatattattc ccgcctcgtt
     4561 tttagccccc agatttgtct tgacgcactc aacgattcta ttctcggcca cgttgactcc
     4621 ctggcgtttt tacagcgaca cgcttggcct gccgaccgac gccgcatggc tcgacgttga
     4681 accacccttc aaaaccggtc aactggcggt tcaagtggtt agcgcgatct ccacccgcta
     4741 tcgggaccgc tcccagtccc tgtcaccgat aacggacgtg atgggccgcc agtttttcaa
     4801 agagcccgga aattatctgg cgtttgcaag cagtttcgat tacctgcggc aattagccga
     4861 tttgtttgag acgcgctacc cgcatattcc tatttggcga cagtcgcgcg gcatggacga
     4921 aacccagcgc acgacgtttc taaatcgctt tactgaaacc ggtcagggaa ttggattcgc
     4981 ggtattgggc ggagctttcg ccgaaggcgt cgacttgcct gggcggcgac tgattggcgc
     5041 atttattgca acgctgggtt tgcccgaaat tagcccggcc aatcaacgat tcatgcaacg
     5101 catggatagc gcctttggcg gaggttacga atacacctat ctttatcccg gtattcgcaa
     5161 ggtggtgcaa gctgctgggc gcgtgatccg cggcgagacc gaccgcggcg tggtgtattt
     5221 gatggacgac cggttcggtc gacgggatgt gcaacgcctg ctgcccaaat ggtggcacgt
     5281 atgcgccttt ccggcgggcg ggcacgatac ttgcagccct ttgaccacca cccgttaatt
     5341 caaacgcctg gaaggggatt atcgtgttgc gtagtctaaa tagcctgaac gcccgaacgg
     5401 atgggccgcg cattgctgtc gcctttccgc gccgcatttc ggcgccaccg cacgaggttg
     5461 cctcgcactg ccatttgacg cagcgtctgg cgatactgtt ggggcgtgat tttattgtcg
     5521 attacgatcc gctggttcgc aacacggtcg acaacattta ctacgtgccc tacagaacgc
     5581 tggtggaacc aggcgctgct ggctcaacag aagaacaact gatgaacctg agcggcctgc
     5641 acgacttgtt tggcggagtc gtgccctacc ccttcgtcgg gacaaaagcc attacccatt
     5701 ctctggtcga ccgcgatgca cgcgcgccaa taggatggtc aaaaaaattc ggtgaacatg
     5761 tgcgccaagc cgtgttgcag ggcgcaaccg ttttttcacc ggaagatgcg cgtcgcgccg
     5821 gcgctgtcct gatacgggcc ggcccggtgc gcattaagcc ggtgctagga atcggcgggc
     5881 gtggtcaaaa tgtggttgag agccaaagcg cgctgaacga cgcaatagaa gaacaaaatg
     5941 ccacagagct aaacgattgc ggtctggtcc tcgagcagca tctggacgac gtctgcacct
     6001 atagcgtagg tcatgtaagc gtgggcgatc ttgttgtcac ctatgtggga acccaaagcc
     6061 tgactcgcga taacgctggc gaaatggtct atggagggtc cgatctcttg ttcgcccgcg
     6121 gcgaattcga agcgctactt gcgattaatt tgaccgatgc cgagcgggaa gcggttcggc
     6181 tggcgcgcat ttacgatgcg gctgcattgc ggtgctaccc cggtttgtat gcatcgcggc
     6241 gcaattatga tgtcgcgcag ggcaaggaca tttggggaag agtccgcact ggcgtattgg
     6301 aacaatcctg gcgcgcgggc ggcgccagcg tggcggaagc ctgcgcactc gaggcgtttc
     6361 accgcgcgcc cgatcttcgg acattgcgcg cctttacctg tgaacgctat ggcgcccagc
     6421 atccccagcc tgcgccggcg caattggtct accgcggcca ggacacggaa attggcttta
     6481 ttaccaaata tggtggaatt tcttcgtatg gcgacaaata gcgaaacaac acatatcacg
     6541 gtacaggatc aacgcatcgc aggcacgttc ctcgttccga aaaacaaggt tccaggtgtt
     6601 ttgttcatac atggctgggg cggcagtcag cagtttgacc ttctgcgcgc caaaagtatt
     6661 gccggtctgg ggtgcatatg tctgactttc gacctgcgcg gccatgccga cacacaagcc
     6721 cagcaaatga aggtgtcgcg cgatgacaac ctgcacgacg tggtggccgc ttacgactgt
     6781 ctgattgcgc acccgtccat cgacacttcc gcggttgtgg tggttggcag cagttatggc
     6841 gggtatctgg ccgcgctatt gacgggttta agacaggtgc agtggctggc actgcacgtc
     6901 ccggcgcttt atcgagacga tgaatggatg ctgccaaaac gccaattaaa ccgggaaata
     6961 cttacggcgt accggctctc gcacgtgggg ccagaagaca atcgcgccct tgcggcatgt
     7021 cgcaacttca gaggcgacgt gctgatcgtc gaagctgaac acgataattt tgtgcctcac
     7081 tccacaatca tgaattaccg cagcgccttt caacattcgc attctctgac ccatcgcatc
     7141 gtggatcagg cggaccatgc cttgaccaat aaggcggctc agcaagctta cacgacgata
     7201 ttgtcgaact ggacaacgga aatggtggtc ggcgcacgcg tgggcagcct gcgcccaact
     7261 taattccgat ctgggatgcc ccggagatgg catctgggca cgaggcttgc atgaacgcca
     7321 aggttcgtac tttgcggagt tgttccaatg cccctgtcca agctcattca gcgcctcaca
     7381 ctgaaggtgt tgactattaa cgcccacaaa ggattcacaa tttttaatcg ccgattcatt
     7441 ctgcgcgaat tgcgcgacgc cgttcgtcag gcggcgccag acattgtttg cctgcaagag
     7501 atattgggcg aggaacagcg ccggacggcc cgaatcaagg aatcgcctgt tttgtcccaa
     7561 tacgaatttt tggccgatac gctgtggccg gagtttgcct atggccgcaa cgccgtttac
     7621 ccgcgaggcc atcatggcaa tgctgtgttg tcgaaatacc caatctcgac atataaaaat
     7681 tatgacgtca gcatcagccc gcacgagggg cgcggtttat tgcattgcat cctcaatcct
     7741 gtggatatgg gtttgaacgt ccacgttatt tgcgtgcacc ttggcttgcg cgagagccat
     7801 cgacggcatc aattgcgacg tctgtgcgac ttggtttcta gcactctgcc caacgacgag
     7861 cctcttatta ttgcgggcga tttcaacgat tggcgtcagc gagccgaagg gttattgcag
     7921 cgctgcgggg tgactgaggt gttcgcttcg tcgctgggac gtccagtgcg caccttcccg
     7981 gcacgctgtc ccttattgcg gcttgatcgt atatatgttc gcaatgtaaa ggcttcccgt
     8041 ccgatcatga tggcggcgcg gccctggtca cgattatcgg accatatcgc actttcggcg
     8101 gagattcagc tttgacgact ccctggcgcg gtggaaatga atttatgctg cttgagaacg
     8161 gggaacacta ctttcctcgc gtgacgcagg ccattggcct ggccaaaact gaaatcctgc
     8221 tcgaaacctt cattttgttc gacgataaag tcggacgcgc gctacgtcag gcgttgatcg
     8281 acgcggcgcg gcgcggagta gaaatcgata tgacggtcga tggctacggt tccgatacat
     8341 tgtcggacga attcatctcg ggcatgactg actgcggcgt acgtttccac gtattcgacc
     8401 cgcgtccgcg tctgctcggc atgcggacga atttgttccg ccgcatgcat agaaagattg
     8461 tttcgattga tggccaattg gctttcgtag gcggcatcaa tttctcggcg gatcacttgc
     8521 cggatttcgg cccggaagcc aaacaggact attcaattga gattcgcgga ccactggcgc
     8581 tcgacatcag gaatctcgct cgcgaggcgc tcgatgtgaa gcaggccggc agacggcgca
     8641 ccagaatatg caggcggccg aaagaagcgc accattcagg cgaagcgcag ggccgtctgg
     8701 tcgtgcgcga caatggcgag caccgtaccg acattgagcg ctcttaccgt gcgggtattc
     8761 gcgccgccaa gaacgacatc ctgattgcca acgcctattt ctttcctggc taccgattac
     8821 tgcgcgattt ggccaaggca gcacgccgcg gggtgcgcgt ccggttaatc ttgcaaggtc
     8881 agccggacat gcttattgcc aggctggcgg cccgcatgct gtatgagtat ctggccgacg
     8941 cgggtgtgcg ggtgtatgaa tactgccagc gaccgctgca tggcaaagtc gcgtgcatcg
     9001 acacgacctg gtccaccgta gggtccagca acctggatcc cttcagtcta gccttgaatc
     9061 ttgaagcgaa tgtttttatt caggacagcg tctttaatgc gacgctgcgt gaaaggctgg
     9121 aagatcttct tgcgtgccat tgccgcccag cccccgcatc gattagctcc ggcgggcatc
     9181 tgcgccggct atgggtgggc gttgtggtgt ttcatttttt gcgccggttt cccgcgtggg
     9241 ccggcagcct gcccgcgcac aagcctgaat tgcattcaat tgccggccgc ggctacgccg
     9301 atacggatac gaaggaggcg acatgagtgc ggttctggca tccatgcgcc aaggagcgtc
     9361 agccgcatgg ccgtggttgg cgcggttgct gaccgcgaca at
//
LOCUS       DATCXU010000239         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_401904,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000239 DATCXU010000000
VERSION     DATCXU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401904"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /gene="mutL"
                     /locus_tag="VIR04_05375"
     CDS             <1..602
                     /gene="mutL"
                     /locus_tag="VIR04_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016498.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY9460210.1"
                     /translation="AASPEEFPLGMALGQLHGIYILAQNQAGLVIVDMHAAHERVVYE
                     QLKQAMDLHDLPRQELLVPVVFNVSEKEIGLAEEHNDTLAELGLTIRPAGPGALMVRA
                     VPALLANGDIESLAREVLRDLAGVGSSARLTEQRNELLSTMACHGAVRANRRLTLDEM
                     NALLRKMEHTERADQCNHGRPTWVFWKLGDLDRLFLRGQ"
     gene            599..1540
                     /gene="miaA"
                     /locus_tag="VIR04_05380"
     CDS             599..1540
                     /gene="miaA"
                     /locus_tag="VIR04_05380"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY9460211.1"
                     /translation="MTLPIICLTGPTAAGKSAAAQALAARWPIEVINVDSATIYRGMD
                     IGTAKPSRAEQRRTPQHLIDILDPANRYSAAEFAQDALRLIADVLQRGRLPLLCGGTM
                     LYYKALREGLNDLPAADPQIRADIDRQARQSGWPELHRQLQAFDPVTARRLAPNDSQR
                     LQRAIEIYRITGLPMSALLNAGHAPKEAAYHYLTVSLEPSERGALHTRIERRYRQMIE
                     DGLIAEVQALHQRPDLHTGLPSMRCVGYRQLWDYLDGASSLDQATEQAIAATRQLAKR
                     QLTWLRAQPERHIIDCLAHDAASLVVDQVARTWPAPV"
     gene            complement(1537..>2448)
                     /gene="purM"
                     /locus_tag="VIR04_05385"
     CDS             complement(1537..>2448)
                     /gene="purM"
                     /locus_tag="VIR04_05385"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY9460212.1"
                     /translation="FGALFEVPKRFKEPVLVSGTDGVGTKLRLAFEWQRHDTVGIDLV
                     AMSVNDILVQGAEPLFFLDYFGCGKLSVETAARVIEGVAKGCELSSCALIGGETAEMP
                     GMYPDGEYDLAGFAVGAVEKSLIIDGRSIVPGDVVLGLASSGAHSNGFSLLRKILTHA
                     NARRDQDLGGRPLEDVVMEPTRIYVKSVLAALAEHGASIKGLAHITGGGLLDNVPRIL
                     RPGLTARLHRDAWTMPELFAWLQENGRISDSEMHRVFNCGIGMVIVVPKDSADAVTAT
                     LRAQGEQVSQVGEIIEQRADAAQTVVI"
BASE COUNT          529 a          798 c          682 g          439 t
ORIGIN      
        1 ccgccgcaag ccctgaagaa tttccgttgg gaatggcgct gggccaactg cacggcattt
       61 atatcctggc gcaaaatcag gcgggactgg tcatcgtcga tatgcatgca gcccacgaac
      121 gagtcgtcta cgagcaactg aaacaagcca tggatcttca cgacctgccg cgccaggaac
      181 tgctggtgcc tgtggtgttc aacgtatcgg aaaaggaaat cggactcgcg gaggagcaca
      241 acgacacgct tgccgagctg ggcctgacca tacggcccgc cggccctgga gcgctcatgg
      301 tgcgcgctgt gccagccctt ctggcaaacg gcgacatcga atcactggcg cgcgaagtcc
      361 tccgggatct ggccggcgtg ggcagttcgg cccggctgac cgagcagcgc aacgaactgc
      421 tctccaccat ggcgtgccac ggcgctgtgc gggccaaccg gcgcctgacg ctggacgaaa
      481 tgaacgcttt gctgcgcaag atggaacaca ccgagcgcgc cgatcaatgc aaccacggac
      541 gccccacatg ggtgttctgg aaacttggcg atcttgacag actgttttta cgcggacaat
      601 gacgctacct ataatctgcc tgaccggccc cactgccgcg ggtaaaagtg ccgccgccca
      661 agcgctggcg gcgcgctggc cgatcgaagt tattaacgtg gactccgcga ccatttatcg
      721 cggcatggat atcggcacgg ccaaacccag ccgcgccgag caacgacgga ctccgcagca
      781 tctgatcgac attctcgatc ccgccaatcg ctactccgcc gcggagtttg cgcaagacgc
      841 cctgcgtctg atcgcagatg tcctgcaacg tggcaggcta ccgctcttgt gcggcggcac
      901 catgctgtat tacaaagcgc tgcgcgaggg gctaaacgat ttgcccgccg ccgatccgca
      961 aatccgcgcc gacattgacc ggcaagcgcg gcaatcgggc tggcccgaac tgcaccggca
     1021 gcttcaggct tttgatccgg tcaccgcccg gcggctggcc cccaacgaca gtcaacgctt
     1081 gcagcgggcc atcgaaattt accggattac ggggctgccc atgtcggcgc tattaaacgc
     1141 cggacacgcg ccgaaagaag ccgcatacca ctaccttacg gtcagcctgg aaccatcgga
     1201 acgaggcgcc ttgcacacgc gtattgaacg gcgttaccgg caaatgattg aagacggtct
     1261 gatagccgag gttcaggccc tgcatcagcg ccccgatctt cataccgggc tcccatcaat
     1321 gcgatgcgtg ggatatcgcc agctttggga ttatctggat ggagcgagca gcctggacca
     1381 ggccaccgag caggcgattg ccgccacgcg acaactggcc aagcgccagt tgacctggct
     1441 gcgagcccag ccagaacgcc atattatcga ttgcctggcg cacgatgcag ccagcctggt
     1501 tgtcgatcag gtcgcgcgaa cctggccggc cccggtttaa attactacgg tttgcgccgc
     1561 gtcggcgcgc tgttcaatga tttcgcccac ttggctcacc tgttcacctt gcgcgcgcaa
     1621 agtcgccgtc acggcgtcgg cgctatcctt cggcacaaca atcaccatgc cgataccgca
     1681 gttaaacaca cgatgcattt cactgtcgga gatccggcca ttttcttgca gccaggcgaa
     1741 aagttcaggc attgtccatg catcgcgatg caaccgtgct gtcaggccgg ggcgcaaaat
     1801 acgcggcacg ttgtccagca agccgccgcc ggtaatatgg gccaggcctt tgattgacgc
     1861 cccatgttcg gccagtgccg ccaaaaccga tttaacatag atgcgagtcg gttccatgac
     1921 aacatcttcc agaggccggc cgcccaaatc ctgatcccgc ctggcattgg catgcgtcaa
     1981 aatcttgcgc aacaaggaaa aaccattgga atgcgcgccg ctcgatgcca gacccagcac
     2041 gacgtctccg ggaacgattg aacgcccgtc aatgatcagc gatttttcga ccgcgcccac
     2101 cgcaaacccg gccagatcgt actcgccgtc agggtacatg cccggcatct cggccgtttc
     2161 gccaccgatc agcgcacacg acgataattc acaccccttg gccacgcctt caataacgcg
     2221 ggcggccgtc tcgaccgaca atttgccgca gccaaaataa tccagaaaaa atagcggctc
     2281 cgcgccttga accagaatat cgttcacgct catggccacc aggtcgatgc cgaccgtatc
     2341 atggcgctgc cattcaaagg ccaggcgaag tttggtgcca acgccatcgg ttcccgacac
     2401 cagaaccggc tctttgaagc gcttgggcac ctcgaaaagc gctccaaa
//
LOCUS       DATCXU010000240         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_413796,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000240 DATCXU010000000
VERSION     DATCXU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413796"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1468
                     /locus_tag="VIR04_05390"
     CDS             68..1468
                     /locus_tag="VIR04_05390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY9460213.1"
                     /translation="MKHVKTGVAVIGAGTAGLNAYRAVKSAGLDAILIEGGPYGTMCA
                     RVGCMPSKLLIAAAAAAHAAAHTAPFGVHIQGSIVVNGREVMDRVKRERDRFVGFVVD
                     GVEQMPANDKIRGYASFVSDTVLRVDDHTEIEAKSIVIATGSTPVVPQMFKALGDRVI
                     VNDDVFAWDDLPQSIVVVGTGVIGLELGQALARLGVRTKVLGRSESLGGLSDPQVKAS
                     ALAAFQAEFDLQLNTRIITARRVGDEVELCYRDAGGQDHTERFGYLLMAAGRRPSVDQ
                     LALQNTSQASDGKGVPDYDARTLQLGSSPIFIAGDVDGVLPILHEAADDGRIAGENAA
                     RFPDVRQARRRAPFSVVFSEPQLAQVGLHFNELPSTGVVIGEVDFGNQGRSRIMLQNR
                     GKLRVYASQPDGLFLGAEMAGPSAEHVGHLLSWAVQQKMTVHKMLEMPFYHPVIEEGV
                     RTALRQAASQIDGRGR"
     gene            complement(1465..2070)
                     /locus_tag="VIR04_05395"
     CDS             complement(1465..2070)
                     /locus_tag="VIR04_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY9460214.1"
                     /translation="MNVAHIRFFRSIVLAFALSLSSLVSAATPLHNVRGFLPDLNFTL
                     EGVGGKTVTQDAFKNKAVLMFFGYASCPDVCPTTLAQLAQVMQKLGPDAKRVSIVFIS
                     VDPHRDTPEKLQAYVNAFDKNAVGLTGSEQQIADVARRYRVAYQIEKPSTADANDYVV
                     AHGRGVYVFDQKGRARLLASDANSIQALTDGVLSVLANARN"
     gene            complement(2082..>2283)
                     /locus_tag="VIR04_05400"
     CDS             complement(2082..>2283)
                     /locus_tag="VIR04_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY9460215.1"
                     /translation="AFEETALDGPDGARDTLNPETGQRFRREILAVGGARPAADSFIA
                     FRGRHANMEALLRHNGMTEQAL"
BASE COUNT          457 a          611 c          734 g          481 t
ORIGIN      
        1 accgggctca agcctgtgtt gccgtaagga ccgctcgcgg cgggaaacca gacggagaaa
       61 ttcacgtatg aagcacgtaa aaacaggggt cgccgtcata ggcgcgggaa cggctggcct
      121 gaacgcttac cgggcggtga agtcggctgg actcgacgcg atcctgattg aaggcggccc
      181 ctatggcacg atgtgcgcgc gcgtgggctg catgccatcc aagttgctga ttgccgccgc
      241 cgcagcggct catgccgccg cgcataccgc gccttttggc gtgcatatac agggcagcat
      301 cgttgtgaac ggccgcgaag tcatggatcg ggtcaaacgt gaacgggatc gttttgtggg
      361 tttcgtggtc gatggcgtcg agcaaatgcc ggcgaatgac aagattcgcg gctatgcaag
      421 ctttgtgtcc gatactgtgt tgcgggtcga tgaccacact gaaattgaag ctaaaagtat
      481 tgttattgcc acgggctcga cgccggtggt tccccagatg ttcaaggcgc tgggcgaccg
      541 ggtaattgtc aacgacgatg tgttcgcgtg ggacgacttg ccgcaaagta ttgtggtggt
      601 ggggaccggg gtcataggtt tggaactggg acaggctttg gcgcgcctgg gtgttcgtac
      661 aaaagtgctg ggtcgcagtg aaagtctggg cgggctgagc gatccgcagg tgaaagccag
      721 cgcactggcc gcctttcagg ccgagttcga tttgcaattg aatacgcgta ttatcacggc
      781 gcggcgggtc ggagatgaag ttgagctgtg ctatcgggat gccggcgggc aggatcacac
      841 ggagcgtttc ggttatttgc tgatggccgc ggggcgccgg cccagcgtcg accagcttgc
      901 tctgcaaaac acgtcgcagg cgtcagacgg gaaaggagtg cctgactatg acgcgcggac
      961 tctgcaactg ggctcttcgc caatttttat cgccggcgat gtagatggcg tccttccgat
     1021 tttgcacgaa gcagcagatg atggacgcat cgccggcgaa aatgcggctc gtttccctga
     1081 tgtccggcaa gcgcggcgcc gcgcgccatt ctcggtcgtt ttttccgaac cgcagttggc
     1141 gcaggtgggc ttgcatttca acgaacttcc ttcaacaggc gtggttatcg gcgaagtcga
     1201 tttcggcaac caggggcggt ccagaattat gctgcagaat cggggcaagc tgcgcgttta
     1261 cgcgagccag cctgacgggt tgtttctggg tgcggagatg gcggggccaa gcgcagaaca
     1321 cgttggacat ctgctgtcct gggcagtcca gcagaaaatg actgtgcata aaatgctcga
     1381 gatgccgttt tatcatccgg ttatcgaaga aggcgtgcgc accgcactca gacaggccgc
     1441 ctcacaaatt gacgggcgcg gccgctagtt cctggcgttc gccagcaccg acaagacgcc
     1501 gtcggtcagt gcttgaatgg aattggcgtc agaggccagc aatcgtgcac ggcctttttg
     1561 atcgaaaacg taaaccccgc gcccgtgcgc aacgacataa tcattggcgt ccgccgtgct
     1621 gggcttttcg atttgatagg cgacgcgata gcggcgcgcg acgtcggcga tttgctgttc
     1681 gcttcccgtg agccccacag cattcttgtc gaaggcattg acgtaggcct gcagcttttc
     1741 gggagtgtcg cggtgggggt ccacgctaat gaacacgata ctgacccgtt tggcgtccgg
     1801 ccccagtttt tgcatgacct gcgcaagctg cgccagcgtc gtgggacaga catcggggca
     1861 actggcataa ccgaaaaaca tcaaaactgc tttgttttta aaggcatctt gggttacggt
     1921 tttgcccccc acaccttcca gggtgaaatt caggtcgggt aaaaacccgc ggacattatg
     1981 caatggcgtg gcggcagaga cgagcgagct caggcttaat gcgaaagcca gcacgattga
     2041 cctgaagaag cggatatggg cgacgttcat gatgaaatca attatagggc ctgttccgtc
     2101 atcccgttgt ggcgcaataa cgcctccata ttggcgtgcc ggccgcggaa tgcgataaag
     2161 gagtccgccg ccggacgcgc gcctccgacc gccagaatct cgcgccgaaa gcgctgtccc
     2221 gtttccggat tcagggtgtc gcgcgcgccg tccgggccat ccagcgcagt ttcttcgaat
     2281 gcg
//
LOCUS       DATCXU010000241         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_714424,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000241 DATCXU010000000
VERSION     DATCXU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714424"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="pheT"
                     /locus_tag="VIR04_05405"
     CDS             complement(<1..1159)
                     /gene="pheT"
                     /locus_tag="VIR04_05405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY9460216.1"
                     /translation="MQFSESWLRTLVNPELDTEALSHQLTMAGLEVESTEPVAPAFSG
                     VVVARIVQVEPHPNADRLHVCRVDDGSGELLQIVCGAPNAAAGVNVPLARIGARLPGD
                     IKIAAVKMRGIESSGMLCSARELGLSQDHAGLLILDADAVIGQPLREFLDLDDTLFTI
                     KLTPNRADCLSILGVAREVAALTGAPLTLPTARPVKPTLTDRLSVSVLAPDLCGRFAG
                     RIVRGVNARAATPGWMKTRLERAGQRSVSALVDVSNYVMLELGRPTHVFDLKRISGGL
                     TVRWARQGETLELLNGQTVTLEPDTGIIAADGVVESLAGIMGGQATAVNLDSTDIYLE
                     AAFWQPDAIMGRARRYKFSSEASHRFERGVDFESVTEHLDFMTRLLIDICGG"
     gene            complement(1206..2228)
                     /gene="pheS"
                     /locus_tag="VIR04_05410"
     CDS             complement(1206..2228)
                     /gene="pheS"
                     /locus_tag="VIR04_05410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY9460217.1"
                     /translation="MISVVDDLIDQAKEQFAQAGDAAALENAKARFLGKQGALTAMLK
                     GLASLDPGQKRTEGARINQLKQQIERLLDERREQLAQIELDKRLAFETIDVSLPGRGR
                     GIGGIHPVIQTWQRVEAIFASIGFDVADGPEIEDDWTNFTALNNPENHPARSMQDTFY
                     IDMKDQHGLPLLLRTHTSPMQVRYARMHTPPIKVIAPGRTYRVDSDATHSPMFHQVEG
                     LWIAENISFADLKGVYTNFLRAFFETEDLSVRFRPSFFPFTEPSAEIDMMFTSGPHQG
                     HWLEISGSGQVHPTVVRNFGLDPERYIGFAFGSGLERLTMLRYGVDDLRLFFEGDLRF
                     LRQFNR"
     gene            complement(2301..2660)
                     /gene="rplT"
                     /locus_tag="VIR04_05415"
     CDS             complement(2301..2660)
                     /gene="rplT"
                     /locus_tag="VIR04_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY9460218.1"
                     /translation="MPRVKRGVTARARHKKVINSAKGYRGRRGNVFRIAKQAVMKAGQ
                     YAYRDRRNKKRTFRALWIARINAACRELGVSYSVFIAGTKKASIELDRKVLADMAVHD
                     KAGFAAVVAQAKSALAA"
     gene            complement(2677..2874)
                     /gene="rpmI"
                     /locus_tag="VIR04_05420"
     CDS             complement(2677..2874)
                     /gene="rpmI"
                     /locus_tag="VIR04_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY9460219.1"
                     /translation="MPKMKTKRGAAKRFKVRASGSIKRGQAFKRHILTKKTTKNKRQL
                     RGSAAVHKADVASVKAMMPFA"
     gene            complement(3006..>3856)
                     /locus_tag="VIR04_05425"
     CDS             complement(3006..>3856)
                     /locus_tag="VIR04_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9460220.1"
                     /translation="SPGTGKSMLAHRLPGLLPRLTTDQALEVAALASVSGREAVMSDI
                     PPFRAPHHSASAPALVGGGASPKPGEISLAHNGVLFLDEFPEFQRRVLESLREPIETG
                     QVSIARASRTLVFPASFQLVAAMNPCPCGWLGHEKMRCVCSPDRIVHYRSKLSGPLLD
                     RIDLQIVLSAFDVDWLEAPPGEASEPVRERVKRCRERQLARQSHSNARLLASELDQYC
                     QLGNAAKNILGHAMRRWSWSTRVVHRVLRVARTLADLNEADVIGRGHIAEAIQYRQPW
                     DAGPTH"
BASE COUNT          861 a         1151 c         1050 g          794 t
ORIGIN      
        1 ggccgccgca aatatcaatc agcaagcggg tcatgaaatc cagatgttca gtcactgact
       61 cgaaatcgac cccgcgttca aagcgatgac tggcctccga gctgaactta tagcggcgcg
      121 cacgacccat gatggcatcg ggctgccaga atgcggcttc caggtaaata tcggtcgaat
      181 ccaggttaac cgccgtggct tgccccccca tgattcccgc caagctttcc accacgccat
      241 cagccgcgat aatgcccgta tcgggttcca gcgtcacggt ttgaccattg agcaattcaa
      301 gtgtttcacc ttgtctggcc caacgcaccg tcaaaccgcc actgatgcgt ttcaggtcga
      361 atacatgggt cgggcggccc aattccagca ttacgtaatt ggacacgtcg accaaagccg
      421 agacactgcg ctggccggcg cgctcgaggc gggtcttcat ccagccgggc gtcgcggcgc
      481 gcgcgttgac gccgcgcacg atccggccgg caaaacggcc gcacaaatcg ggggccagaa
      541 cggaaacaga caagcgatcg gtcaaggtag gcttaacggg ccgggctgtc ggcaaagtca
      601 gcggcgctcc ggttaatgcc gcgacctcgc gcgccacgcc cagaatggac aggcaatcag
      661 cgcggttagg cgtcaacttt atcgtgaata acgtgtcgtc cagatcgaga aactcacgca
      721 ggggctgtcc tatgacggcg tcagcatcca ggataagcaa gcccgcatga tcctgcgaca
      781 ggcccagttc acgcgccgaa cacagcatgc cgcttgattc gattccgcgc atcttgaccg
      841 ccgcgatttt gatatcgccg ggcaagcgtg cgccaatacg ggccaaaggt acattgaccc
      901 ccgccgccgc atttggcgcc ccgcaaacga tttgcaataa ctctcccgat ccgtcgtcaa
      961 cccggcacac atgcagcctg tccgcattag ggtgcggctc aacttgaaca atccgggcca
     1021 ccacaacccc actgaacgcc ggcgcgacag gctcggtgct ttcgacctca aggccggcca
     1081 tcgtcaattg gtgagataag gcttcggtgt ccagctcggg gttaacaagt gtgcgtaacc
     1141 aagattcaga gaattgcata gtgtgatgtc aggttcgttg aactacagcg ggactcgggc
     1201 gatggctaac ggttgaactg acgcaaaaag cgcaggtcgc cttcgaaaaa caggcgcaag
     1261 tcatcgacgc catagcgcaa cattgtcaaa cgctccaggc ccgatccgaa cgcaaaacca
     1321 atgtagcgtt ccgggtccag cccaaaatta cggacaacgg tcggatgcac ctgcccggac
     1381 cctgaaattt cgagccaatg accttggtgc ggccccgacg tgaacatcat gtcgatttca
     1441 gccgaaggct cggtaaacgg gaaaaacgag gggcgaaagc gcaccgacag gtcttccgtt
     1501 tcgaaaaatg cccgcaagaa attcgtgtag acgcctttga ggtcggcaaa ggagatattt
     1561 tcggcaatcc acaaaccctc cacctgatgg aacatcggtg aatgcgtggc gtcgctgtca
     1621 acccgatacg tgcgcccggg agcaattact ttaataggcg gagtgtgcat gcgcgcatag
     1681 cgcacctgca tggggcttgt atgtgtgcgc agcagcaacg gcaatccgtg ctggtctttc
     1741 atgtcgatat aaaacgtatc ctgcatggag cgcgcaggat gattttcagg attgttcagc
     1801 gcggtaaagt tggtccagtc atcttctatt tcagggccgt cggccacatc aaacccaata
     1861 gaggcgaaaa tcgcttcgac gcgttgccaa gtctgaatca cggggtgaat gccgccaatc
     1921 ccgcgtccgc gccccggcaa ggaaacatca attgtttcga aagccagccg cttgtcgagt
     1981 tcgatttgcg ccagttgctc gcggcgttcg tccagcaatc gttcgatctg ctgcttgagc
     2041 tgattaatgc gcgcgccctc ggttcgcttc tggccgggat caagactagc cagacccttc
     2101 aacatggccg tcaacgcgcc ctgtttgccc agaaatcgag cttttgcgtt ttcaagcgcc
     2161 gccgcatcgc cggcctgcgc aaactgttct tttgcctgat cgatcaggtc gtcaaccaca
     2221 gaaatcataa aagtcgcttt tccaaatgca aacggagccc ggatagagcc ccgttcgccg
     2281 ccgtacagaa tcgaaaccga ttacgctgcc agggcggatt ttgcctgagc aaccacggcc
     2341 gcgaagcccg ctttatcgtg cacagccata tcggccagca ccttgcgatc gagctcgatt
     2401 gaggcttttt tcgtgccggc gataaacacg ctgtacgaca cgccgagctc acggcaggcg
     2461 gcattgatgc gcgcaatcca caaagcacgg aacgtgcgct ttttattgcg gcgatcgcga
     2521 taggcatact gccccgcctt catgaccgcc tgtttggcga tacggaacac attaccgcgg
     2581 cgaccgcgat aacctttagc cgagttaatt acttttttgt gacgagcacg ggcagtaacg
     2641 ccgcgtttta cgcgaggcat agtagatctc caataatcag gcgaaaggca tcattgcttt
     2701 aaccgaggcc acatcagctt tgtgcaccgc ggccgaaccg cgcagttggc gcttgttctt
     2761 ggttgttttc ttggtaagaa tatggcgctt gaatgcctga ccccgcttga tcgatccgct
     2821 ggcacgaacc ttgaagcgct tggcagcgcc tcgtttcgtt ttcattttgg gcatgaaagt
     2881 aactctgttg atttatgacg gcgggtgatc gccacgcata ctggcaatac ttgtacgacc
     2941 cgatagccac ttattgaact gcttttaaaa agcccttgat tatatgacgg tttttgtgcc
     3001 gctgtctaat gcgttgggcc tgcgtcccat ggttgcctgt attgtattgc ctcggcgata
     3061 tgcccgcgac cgatcacatc ggcctcgttc agatcagcca atgtgcgcgc aacgcgaagc
     3121 acccgatgca ccacgcgagt ggaccagctc cagcgccgca tggcgtgccc aagtatattt
     3181 ttagccgcat tgccaagctg gcaatactga tccaattcgc ttgcaagaag cctggcgtta
     3241 ctatgcgact gccgcgccaa ctgacgttcg cggcaccgtt tcacccgttc gcgcacaggt
     3301 tctgaggcct cgcccggcgg cgcctccagc cagtcgacat cgaacgcgga caagactatt
     3361 tgcaggtcta ttcgatccag taatggcccc gataatttac ttcgataatg cacgattcgg
     3421 tcagggctgc agacgcagcg cattttttca tggccaagcc acccgcaagg acacgggttc
     3481 attgccgcta ctagttgaaa ggaagccgga aacacaagcg tccgcgaagc gcgcgcgata
     3541 gacacctggc cggtctcgat gggctcgcgc agtgactcaa gcacacgccg ctggaattcg
     3601 ggaaactcgt caagaaataa cacgccgtta tgcgccagac taatttcgcc aggcttgggg
     3661 cttgcacccc ctccaacaag agccggcgcc gaggcggagt gatgcggcgc tcgaaacggc
     3721 ggtatatcgc tcattactgc ttcgcgacca ctgacgcttg ccagcgcagc gacttcgagc
     3781 gcctgatccg tggtcaggcg cggcaacaag cccggcagac gatgcgccaa catgcttttg
     3841 cctgtgccag gcgacc
//
LOCUS       DATCXU010000242         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_426296,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000242 DATCXU010000000
VERSION     DATCXU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426296"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VIR04_05430"
     CDS             complement(<1..295)
                     /locus_tag="VIR04_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460221.1"
                     /translation="MANPDGHTSMAAAQEPVLVQRSDTLFDGGRLLGALYIITFIIVA
                     LLVVIPLVALIYGSLRSAAPGLPGQWTFENWAGLVSPGVVSTFVTTIGIGVAAS"
     gene            complement(371..1477)
                     /locus_tag="VIR04_05435"
     CDS             complement(371..1477)
                     /locus_tag="VIR04_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460222.1"
                     /translation="MNTHHLSPPNLLRKHTVGARILGLVAAFAASVAFAPLSAVAQNT
                     APTADQWKAVEAAAKKEGKVAVYHNLNPSGAELLANEFRKDRPDIDIEMTRLGSAPLI
                     QRFGTEFTSGRNIADAVITFPDQRIFDGMKAGWMAAWVPPELKAFPPAVNYENKNMLF
                     NIQTAREAIIWNKQKVKPADAPKEWSDLFDPRWKGKVGMNPPWRSVSIQGVVALWEKL
                     GLGDTAAKLKANNVRFFEGSGGILQAVIRGDIYVSEITDLPVNVALADGAPIGFIYPK
                     SGTSLSEGYIFVAAKAPHPNAGRVFANWLLTERGQTLLQKFAGLSATRPGIPPLSHLP
                     ATSTLSNTVNGLSLTPPEKQKEIVDHWRTVFGVQ"
     gene            1856..2467
                     /locus_tag="VIR04_05440"
     CDS             1856..2467
                     /locus_tag="VIR04_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY9460223.1"
                     /translation="MSALQMNSRKAPIHARPQSIAELYTCFTWLAVQGFGGAAPVAQR
                     ELVERKHWLTQQEFLEDWAVAQIMPGPNVVNLSIMLGDRYFGLRGAMAALAGMISIPL
                     LLVLAMTILYTEYSHYDVIVRALRSMGAVAAGLIIGTGFKLFSTLKSNPIGLPAASVL
                     IVFTFVVVALLRWPLLVVLLITGIPATIWAAMQLARHERKERT"
     gene            2464..>2945
                     /locus_tag="VIR04_05445"
     CDS             2464..>2945
                     /locus_tag="VIR04_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY9460224.1"
                     /translation="MNWNLNIADWLALSWHFLSLSLLTVGSGSLTTAPQMHNYLVDER
                     HWLTNVQFNTSITIAQAAPGPNMLYIALLGWNVGMATHSWTGSIVAMTTAMAGSLLPS
                     TIVTLAGTRWGHRNRNRRTVRAFKAGLAPITTALLIATGWLLMRPYNQPGRDWPLWIA
                     T"
BASE COUNT          649 a          853 c          797 g          646 t
ORIGIN      
        1 gactggcggc cacgccaatg ccaatcgtcg taacgaaggt gctgacgacg cctggcgaga
       61 ccagacctgc ccagttttcg aaggtccact gacctggcaa acccggcgcg gcgctgcgca
      121 ggctgccata aattaacgcc accaggggaa tgacaaccag cagggcaacg atgataaacg
      181 tgatgatgta aagcgcgcca agcaaacgcc cgccatcgaa caaagtgtcc gacctttgca
      241 ccagcacggg ttcctgtgcc gccgccatag aagtatgacc gtccggatta gccatgctca
      301 gcctttcgct ctgttgttcg tttcgggttg cccgacaagc gcctgggtcg ggcaggcgcc
      361 gtctttgcga ttactgaacg ccaaatactg tgcgccagtg atcaacgatc tctttttgct
      421 tttccggagg agtcagcgaa agaccgttaa ccgtgttgga caatgtcgaa gtcgcaggca
      481 aatgcgacag cggaggtatg ccaggtcgcg tagccgaaag cccggcaaac ttctgcaaca
      541 gggtttgccc cctttccgtc agcagccagt tggcaaagac cctgccggca ttcggatggg
      601 gagccttggc ggcaacaaag atatagccct cggacaacga cgtgcccgac ttcggataaa
      661 taaacccgat cggcgcgcca tcggccaacg caacgttcac gggcaagtcg gttatttcag
      721 aaacatagat gtcgccacga atgacggctt gcaatattcc gccagaacct tcaaaaaagc
      781 gcacattatt ggccttgagc ttggccgccg tatcgcccag accaagcttt tcccacaggg
      841 cgacaacgcc ctgaatcgag accgatcgcc agggcgggtt catgccaacc ttgcccttcc
      901 agcgcgggtc gaacagatcg gaccattctt tgggcgcatc ggccggctta accttttgct
      961 tattccagat tattgcttcg cgcgccgtct gaatattgaa cagcatgttc ttattttcgt
     1021 aattgactgc cggcgggaac gctttgagtt ccggcggcac ccacgcagcc atccatcccg
     1081 ctttcattcc gtcgaaaatg cgctggtcgg ggaaggtgat aactgcgtcg gcaatattgc
     1141 gtccggacgt aaattcagtg ccaaaccgct gaatcagcgg cgcgcttcca aggcgcgtca
     1201 tttcaatatc gatgtccggc cggtctttgc ggaattcgtt cgccaacaac tcggcgcccg
     1261 acgggttgag gttgtgatag acggcaacct tgccctcttt ttttgcggcc gcctccacag
     1321 ccttccattg gtccgcggtg ggcgcggtat tttgagcaac cgcactcagg ggcgcaaaag
     1381 ccacgctggc ggcaaatgcc gcaacgagcc caagaatgcg ggcgccgacg gtgtgcttgc
     1441 gtagcagatt aggcggcgaa aggtggtgag tgttcattgt tctgtctcct ttatattgtc
     1501 gtaattgttt gcttgcaatc ttaaggtgcc gccccgcgaa cgggcttgcc gtcagcggta
     1561 cacaggacaa gtcttgcaat gagtgcggat gcgcgcgcac tacaagcgat atcagccaat
     1621 ttattattta ctattttgcg catcatgtgc tcgtcttgcc cgaaatattc atgggtcctc
     1681 ccttcgccaa ccagcgatac ccatgctgaa caaaaaccga tattcaccct gcaaatctgc
     1741 tttgaacagt aacacgagaa acacgtgccg ggctagcgat tatcaatgca tacaatcgta
     1801 taatggcccg gagccaaatt catgtcaaga agtaattcac ccaagaaaac gcctaatgag
     1861 cgcactgcag atgaacagcc gcaaagcacc cattcacgct cgtccgcagt ccattgccga
     1921 gctgtacaca tgctttacct ggctggccgt gcaaggcttt ggcggcgcgg cgcctgtggc
     1981 gcaacgcgag cttgtcgaaa gaaaacactg gctgacgcaa caggaatttt tggaggactg
     2041 ggctgtggcg caaatcatgc ctggcccgaa tgtggtgaac ctgtcgatca tgctgggcga
     2101 ccgctatttt ggcttgagag gagccatggc cgcactggcc ggcatgatat caatccctct
     2161 gctgcttgtg cttgccatga ccattctgta caccgagtat tcgcactatg acgtcatagt
     2221 gcgcgctttg cgcagcatgg gcgccgtggc ggccggacta atcatcggca cagggttcaa
     2281 actgttttca acattaaaaa gcaacccgat cgggcttccc gcggcaagcg tacttattgt
     2341 tttcaccttc gtcgtcgttg cgttgctgcg ctggccgctg ctggtggttc tattaatcac
     2401 cggcatacct gcaacgatct gggccgcgat gcaattggcg cggcatgaac gcaaagagcg
     2461 cacatgaact ggaatctcaa tatcgccgac tggctggcac taagctggca ttttttgtcg
     2521 ctgtcattac taaccgtggg atcagggtcg ctgaccaccg ccccgcagat gcacaattac
     2581 ctggttgatg aaagacattg gctgacgaac gttcaattca atacttcgat cacaatcgct
     2641 caggccgctc ccggcccgaa catgctctac atagcgttgc tgggctggaa cgttgggatg
     2701 gcgactcata gctggacggg ttcgatcgtc gccatgacca ccgccatggc cggttccctg
     2761 cttccaagca ccattgtcac tttggcgggt acacggtggg gtcatcgcaa ccgcaacagg
     2821 cgcacggtgc gcgccttcaa ggcaggcctg gcgcctataa ccactgctct gctgatcgcc
     2881 accggatggc tgctgatgcg cccctacaat cagcccggcc gcgattggcc actatggata
     2941 gcaac
//
LOCUS       DATCXU010000243         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_315524,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000243 DATCXU010000000
VERSION     DATCXU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315524"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VIR04_05450"
     CDS             complement(<1..543)
                     /locus_tag="VIR04_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY9460225.1"
                     /translation="MSAIPLFFPWQRDRASAWLSQRERFAHAWLIHGMAGIGKRQFAL
                     AAAASLLCESPRHGLACGRCAACVWVASGNHPDLRRIRPDATAFEEGGPSDESDSGAG
                     ATKKTLSREIRVDQLRGMASWFNTATHRGGWRVAVLYPAQSLNTISANALLKVLEEPP
                     DHTVFLIVADAPDRLLPTLVS"
     gene            complement(540..1196)
                     /gene="tmk"
                     /locus_tag="VIR04_05455"
     CDS             complement(540..1196)
                     /gene="tmk"
                     /locus_tag="VIR04_05455"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY9460226.1"
                     /translation="MQDQRGCFITLEGLDGAGKSTHAPWLVEEIRAHGLKVISTREPG
                     GTALGEQLREILLHQSMHLKTETLLVFAARCEHVQTVIKPALASGSWVVCDRFTDASY
                     AYQGGGRELGDANIAVLEQWVHPDLQPDRTWLFDVPLAVARERLAHTRDLDRFEREGA
                     PFFERTRQAYHARVKQNSVRFRIVDSTLPIDVIRQELASQVAALIKTYRHPRAPEQDR
                     "
     gene            complement(1196..2206)
                     /gene="mltG"
                     /locus_tag="VIR04_05460"
     CDS             complement(1196..2206)
                     /gene="mltG"
                     /locus_tag="VIR04_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY9460227.1"
                     /translation="MKKFKLIALYAALFVVLALLAATAASWYWANNPVRLDGAQIDYI
                     VEQGSRPRAIARLMNQSGIHINEDGFVLLARLSGLDRKIKAGAYEAAQGDTPQKLLER
                     MANGEMTQTQITLVEGWTYQRIRQALREDPLLKQTLNGVSDEAILKRLKSVYDYPEGL
                     FYPDTYVFAPGSSDYDILRRAHHAQVQLLDAVWAGRDPDLPFQTPYQALVLASIIEKE
                     TGDKSDRERVAGVFVNRLRLNMPLQTDPTVIYGMKDAYQGQIRKNDLTTDTPWNTYTR
                     PGLPPTPIASPGKASLLAALHPEANKYLYFVSRGDGTSEFSETLADHNRAVAKYILKP
                     GR"
     gene            2347..>2760
                     /locus_tag="VIR04_05465"
     CDS             2347..>2760
                     /locus_tag="VIR04_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY9460228.1"
                     /translation="MNDYLNVAPLSDQIVIEAAGPDCVSFLQSQLTQDMAQLQADSAR
                     LAGYCTAKGRLVASTVAWKPESIQAPLVHLLVKADIAAEFIKRLSVYILRAKVKLRIT
                     DSTVHGILIQQTTSGGVRSLGLPRSARPWTVVQSDK"
BASE COUNT          600 a          818 c          761 g          581 t
ORIGIN      
        1 cgacaccaat gtcggcagca gccggtcggg cgcgtctgcc acaatcagaa atacagtgtg
       61 gtctggcggc tcctcaagca cctttaacag cgcgttggcc gaaatggtgt tcagcgattg
      121 ggccggatac agaaccgcca cacgccaacc gccgcgatgc gtggccgtat tgaaccacga
      181 ggccatccct cgtagctggt cgacccggat ttccctggac aaggtttttt ttgtcgcgcc
      241 cgcgcccgaa tcgctctcat cggacggccc gccctcttca aaagccgtcg catcgggacg
      301 aatccggcgc aaatcagggt gattgccgct agccacccat acgcacgcgg cgcaccgccc
      361 gcacgccagg ccgtggcgcg gcgactcgca cagaaggctg gccgccgccg ccaaggcaaa
      421 ctgacgcttt cctatacccg ccatgccatg tattaaccag gcatgggcaa aacgctcgcg
      481 ctggcttagc caggcgctgg cgcggtcgcg ttgccaggga aaaaagagcg gaatggcgct
      541 catcgatcct gctccggcgc ccgaggatgg cggtacgtct taattagcgc tgcgacctga
      601 gaagccagtt cttggcgtat gacgtcgatg ggcaaggtcg aatcgacaat ccgaaaacgc
      661 acggaatttt gtttgacgcg cgcatgatac gcttggcggg tgcgctcaaa aaacggcgcg
      721 ccttcacgct caaaccggtc caaatcacgc gtgtgcgcga gccgttcgcg cgccaccgcc
      781 agcggcacgt cgaacagcca cgttcgatca ggctgcaaat cgggatgcac ccattgctcc
      841 aatacggcta tattcgcgtc gccaagttcg cgcccaccgc cctgatacgc atagctggcg
      901 tctgtgaacc gatcgcacac aacccagctc cccgaagcaa gcgcgggctt gatgactgtt
      961 tgcacatgct cgcagcgggc tgcaaagacc aacagcgttt cagttttcaa atgcatggat
     1021 tgatgcagca agatttctcg caactgctct cccagcgcag ttccccccgg ctctcgcgtg
     1081 gagataacct ttagcccatg ggcgcgaatt tcctcaacca gccacggcgc atgggtgctt
     1141 ttacctgccc cgtccaggcc ttcaagagtg atgaagcatc cgcgttggtc ttgcattagc
     1201 gtcccggttt caaaatgtat ttggcaaccg ctctattatg atctgccagc gtttccgaaa
     1261 actcgctggt gccatcgcca cgcgatacaa aatacaagta tttattcgct tcggggtgca
     1321 gggcagccaa cagcgaagcc ttgcccgggc tggcaatggg cgtgggcgga aggcccggtc
     1381 gcgtataggt gttccatggc gtatcagtcg taagatcgtt tttacgaatt tgaccctgat
     1441 aggcgtcctt catgccgtag atgacagtcg gatcggtttg caaaggcata ttaaggcgca
     1501 gacgattgac gaataccccc gcgacacgct cgcggtcgga tttgtcgccc gtttcttttt
     1561 caataatgga cgccagaacc agtgcctgat acggcgtctg aaaaggtaaa tcgggatcgc
     1621 gccccgccca gacagcgtcc agcaactgca cctgagcgtg gtgggcgcgg cgcaagatat
     1681 cgtaatcgct cgaacccgga gcaaaaacat aggtgtccgg ataaaacagg ccctcggggt
     1741 aatcgtacac ggacttcaag cgtttaagga tcgcttcatc cgatacgcca ttcagggttt
     1801 gctttaacag cgggtcttcc cgcagggcct gacgaatgcg ctgataggtc catccttcaa
     1861 ccagcgtgat ttgggtctgc gtcatttcgc cattggccat gcgttcaagc agtttttgcg
     1921 gtgtatcgcc ctgcgcagct tcataggcgc cggccttgat ctttctgtcc agaccgctga
     1981 gccgcgccaa cagcacaaaa ccgtcctcgt taatatggat gcccgactga ttcatgaggc
     2041 gtgcaatcgc acgcgggcgg ctgccttgtt cgacaatgta gtcaatctgc gcgccgtcca
     2101 gccggactgg attattggcc caataccagg aggcagcggt ggcggccagc agcgccagaa
     2161 cgacgaacag ggctgcatac agagcaatca gtttaaattt tttcatcagg gcgaagtgta
     2221 gccgacctat tcatcggcaa ggtggattcc aggtaacgaa tcggatgaga tcgtcagcaa
     2281 agttattcaa agcccgctat catgaacgcg tcttatgcgt gtttttgttg cggtacttgt
     2341 acctttatga atgattattt aaatgtcgcg ccgctatccg atcagatcgt catcgaagca
     2401 gcaggcccgg actgcgtcag cttcttgcaa agccagctta cccaggacat ggcccaattg
     2461 caggccgatt cggcgcgcct ggcgggatac tgcacagcca agggccgact tgtggccagc
     2521 acggtggcct ggaagccgga atcaattcaa gctcctttgg ttcatcttct ggtcaaagcc
     2581 gacattgccg cggaatttat caaacgcctg tcggtgtaca ttctgcgggc caaggtcaaa
     2641 ctgcggatca cggattcaac ggtacacggc atcttaattc aacagacaac atcaggcggc
     2701 gttcgttcac tcggcctgcc cagatctgcc cgcccctgga cagtcgtgca atccgacaaa
//
LOCUS       DATCXU010000244         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_504964,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000244 DATCXU010000000
VERSION     DATCXU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504964"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VIR04_05470"
     CDS             complement(<1..251)
                     /locus_tag="VIR04_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460229.1"
                     /translation="MKSINSFGARDELKVGAKSYRIYRLDAVKGKGLDVATLPYGLKI
                     LLENLLRTENGSDVTADDIRALAGWNAAAAPDREIAFTPA"
     gene            complement(367..1368)
                     /locus_tag="VIR04_05475"
     CDS             complement(367..1368)
                     /locus_tag="VIR04_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY9460230.1"
                     /translation="MKAIRIEQYGGAEVLLHQDLPVSAPKQDEVLIRVAFAGINFMDV
                     HTRQGKYAASRTYPIRLPLTLGMEGSGEIVAVGDGVRSLKIGDRVAWCLAWGSYAEYA
                     VVPAARVAKIADAVPFDLAAAAMFQGCTAHYLSHDVGQLEPGRTCLIHAASGGIGQLL
                     IQFAKLAGATVYATTSSAAKAEVARRRGADHVMLYDGGRFADAVRQATGGVGVDVVFD
                     AIGRTTLRDSFRAVRKRGLVVNYGSVSGSITDLDPIELGEAGSLFLTRPRLGDYLADA
                     NTVQHRADDVFAGLLDGSLSVSIDARYTFANFALAHAALEERKQLGKAVLDVRAPCI"
     gene            1580..2284
                     /locus_tag="VIR04_05480"
     CDS             1580..2284
                     /locus_tag="VIR04_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9460231.1"
                     /translation="MNAKQSPTADGDESIANSRQGSLAEYVYAQLRQEIRNRQLTSGE
                     RLREVDIAARLSVSRTPVREALKRLEAEGLVHFAPPRGFVVVQLSSAQVMELYAMRKV
                     LVGAAVRFAAEQASPIEIQSMQQILSQLERAKKPDNVATINRRLHEAIVAAAHNEYLY
                     KAANVLSDALGLLGTTTYSMPGRIKAGLEENKKIVACITAHDPVGAEQAAHEHISAAT
                     ELRLKMLFGAGTDTKN"
     gene            complement(2317..>2599)
                     /locus_tag="VIR04_05485"
     CDS             complement(2317..>2599)
                     /locus_tag="VIR04_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY9460232.1"
                     /translation="AVLRSETLGVRAAGVPAAGDPAAGDPADGDSSLNALHGTVRNLQ
                     YMGPHIEYSVDVSGVLLNALCVDEFPKGAAVLVTFRPQDIHLLPKPAAA"
BASE COUNT          612 a          786 c          698 g          503 t
ORIGIN      
        1 gccggcgtaa atgcgatttc gcgatccggt gccgcagcgg cgttccagcc ggcaagcgcc
       61 cgaatgtcat ccgccgtcac gtcgctgccg ttttccgtac gcaacaaatt ttcaagcaga
      121 attttcaagc cataaggaag cgttgcaaca tcaagcccct tgccttttac cgcatcgagc
      181 cgataaatac ggtaggattt ggccccgacc ttgagttcgt ctcgagctcc gaaactgtta
      241 atacttttca ttacgcgatc tccttaagac tatcaatgct ttttatgact gcaaattcat
      301 tcaggcatgc aaacatatta caacgatatc aaggctgaac tggtcccggc cgtctggccg
      361 gcgggttcaa atacaggggg cgcgcacgtc aagcacagcc ttgcccaact gctttctctc
      421 ttcgagtgcg gcgtgcgcca aggcgaaatt ggcgaacgta taacgcgcgt cgatcgaaac
      481 gctcaatgac ccatcaagca agcctgcgaa tacgtcatcc gccctgtgct gcacggtatt
      541 tgcatcagca aggtaatcac ccagccgcgg gcgcgtcaga aacagcgagc ccgcctcgcc
      601 cagctcaatt ggatcaagat cggtaatcga gcccgatacg gagccataat ttacgaccag
      661 ccctcgtttc cgaactgcgc gaaagctgtc tcgcagcgtg gtccggccaa ttgcgtcgaa
      721 aacgacgtca acaccgacgc cacccgttgc ctgcctgaca gcgtcggcaa agcggccgcc
      781 atcgtagagc attacatggt ccgcaccgcg gcgccttgca acttccgctt tcgccgctga
      841 actcgtcgta gcataaacgg ttgctcccgc aagcttggcg aactgaatta aaagctgtcc
      901 gatgccgccc gacgcggcgt ggatcagaca tgtccggccc ggttcaagtt ggccgacgtc
      961 atgacttaga taatgcgcgg tgcatccctg aaacatcgcg gcggcggcaa gatcgaacgg
     1021 gacagcatcc gcaattttgg caacgcgcgc ggcagggacg accgcgtatt cggcgtaact
     1081 gccccatgca agacaccagg ccacgcgatc gccgatcttc aacgagcgga ctccgtcgcc
     1141 gaccgccaca atttcgcccg agccctccat gcccagcgtc aagggcaggc gtattggata
     1201 agttcttgat gcggcatatt tgccctgccg cgtatgaacg tccatgaagt tgattccggc
     1261 aaatgcaacc cgtatcaaga cctcgtcctg tttgggcgcg gacacgggga gatcctggtg
     1321 caacaaaact tcggcgccac catactgttc aatacgtatt gccttcaatt cgcggcctct
     1381 gcatcatgat gctgtatgaa acgctcaacg aaccatcagc aggctcgcgc acgcgctgat
     1441 tgtactcgcc agccgtgcat gtatacgcgg acatacgata aaatggaggt gtgcaaagac
     1501 cctgatacca ggtcgtcgac ggcgctatcg ccggaatacg catgtatact ttgcctcaca
     1561 caacacaaag gtgcgaagcg tgaatgcaaa acaaagtccg accgcggatg gcgatgaatc
     1621 gatcgcaaac tcaaggcaag gctcgctggc ggaatacgtt tatgcacaat tacgtcagga
     1681 aatccgcaac cggcaattga catcaggcga acgcttgcgc gaagttgata ttgccgcacg
     1741 tctaagcgta agccgcactc cggtgcgcga agcgctgaag cggctggaag ccgagggtct
     1801 ggtgcatttc gcgccgccac gcgggtttgt ggtcgtacag ttgtcttcag cacaggtgat
     1861 ggagctgtac gcgatgcgca aggtgctcgt tggcgcggcg gttcgtttcg cggccgagca
     1921 agcgtcgccg attgaaatcc agtcaatgca gcaaattctc tcgcaattgg agcgcgcaaa
     1981 aaaaccggac aacgtagcca cgatcaatcg cagactgcac gaagcgatcg tcgccgccgc
     2041 ccacaatgaa tacctttaca aagccgccaa tgtcctgagc gatgcgctgg gcttgctcgg
     2101 aaccacgacg tattccatgc caggccggat caaagccggt cttgaggaaa acaagaagat
     2161 cgttgcatgc attacggcgc acgatcccgt cggcgccgaa caggccgcgc acgaacacat
     2221 cagcgcggcc accgagctaa ggttaaagat gctgtttggc gccggaaccg acacaaaaaa
     2281 ctgactggcg ttcgattacc gttcacccag ttttcatcac gccgctgcag gtttaggcaa
     2341 aagatggata tcctgaggcc ggaaagtcac cagcaccgcc gcgcctttgg gaaactcgtc
     2401 aacacacaag gcatttagca gcacgccgct gacgtccacc gaatactcga tatgagggcc
     2461 catgtactgc aaattcctca cagtgccgtg cagcgcatta agactcgagt cgccgtccgc
     2521 gggatcgccc gccgcgggat cgcccgccgc cggtacgccg gccgctctga cgcccagggt
     2581 ttcggaacga agcaccgct
//
LOCUS       DATCXU010000245         5187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_949201,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000245 DATCXU010000000
VERSION     DATCXU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949201"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VIR04_05490"
     CDS             <1..482
                     /locus_tag="VIR04_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9460233.1"
                     /translation="IWGLQPGPTLFVEQKDFVWGLIASMYLGNVVGLIVVLFTVPLFA
                     SILRVPFSIMAPLIVIVCSIGAYAVNQSLFDVGMMLVFGVFGYLFKKLGYPIAPLVLA
                     AVLGDKAEDAFRQSMLFSGGNLGIFFHNGLAGTLTILGLVMAFLPVIGRLFKRNNPT"
     gene            538..1581
                     /locus_tag="VIR04_05495"
     CDS             538..1581
                     /locus_tag="VIR04_05495"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9460234.1"
                     /translation="MSEQKPTVALTLGDPAGIGPELIAKLLNEPSNPEHANIVLVGDP
                     WLWQAGQRIAGVTVTTHEIPSLADARRNACDKPLFVAVDSIAASQVQVGQSAATGGAS
                     VLKILDLCMDAAIARQIDAICFAPLNKHAMKQSGMRFQDELHHFANYLKVAGYFCEFN
                     TLDGLWTARVSSHVPLKDAASHVSKERIEDASRLIFDSLKAAGTAHPRVAVAALNPHG
                     GDGGSCGREEIDIIEPAVRDLNRQGYDVDGPFPADTIFIKAREGKYDAVVTMYHDQGQ
                     IALKLLGFEKGVTVQGGLPIPITTPAHGTAYEIAGKNQATVTAMRNAFLIACRMGNAY
                     RKQAACQTSGTPI"
     gene            1594..2766
                     /locus_tag="VIR04_05500"
     CDS             1594..2766
                     /locus_tag="VIR04_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEY9460235.1"
                     /translation="MKIVNVRARVFEWKGKTTPPQAHFCANAMDLLWDRGDSMGTFRF
                     HGWTVVEIETDDGLTGIGNAALAPRIAKHIIDQYLAPLVTGQDPFDYEYLWQRMYRST
                     LAWGRKGITMAAISAIDIALWDLMGKATGRPVFKLLGGRTKEKIPCYASKLYHNDLGE
                     MQREAQSYLDQGFSAMKLRFGYGPKDGPAGMRKNIESVDAVREVIGYDTDLMLECYMG
                     WNLDYAKRMLPKLERFEPRWLEEPVIADDIDGYAELNKLTAIPISGGEHEFTSYGFRQ
                     LLDKRAVSVIQYDTNRVGGITMAHKINALAEAYSVPVVPHAGQMHNYHLTMSTLASPL
                     SEFFPVHDVEVGNELFYYIFDGDPVPKNGYLDLDDTLPGLGLTLSDKYLDQFNIIE"
     gene            2872..4443
                     /locus_tag="VIR04_05505"
     CDS             2872..4443
                     /locus_tag="VIR04_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEY9460236.1"
                     /translation="MKISGTMLIGAKDVVGTKKDIKAVNPATDETLEPTFKGGTSAEV
                     EQACQLAHSAFQSYRETSLEQRAVFLEKIADHIMALGDELIVRTHLESGLPKGRIEGE
                     RGRTVNQLRLFASVVRDGRWIDARIDFAQPERKPMPRSDIRLRYIPLGPVAVFGASNF
                     PLAFSVAGGDTASALAAGAPVIVKAHSAHPGTSELVGRAIRAAVAECGLHEGVFSLIF
                     GAGADVGQTLITHPHIKAVAFTGSRTAGVALMHSAAARPEPIPVYAEMSSINPVFLMP
                     GALKANAEKVAQGFVASLLMGAGQFCTNPGLAIGIDSPELDAFIRAAEAGVVASPAAT
                     MLTSNIHKAYETNLNQLVLNPNITVLARGKTSDAPNQCRSALLCTSASAFLKDPHGLG
                     IEVFGSSSVVIKCANSDEMLAIVQALEGQLTATLQIAAGQDEALTTSLIAALELKVGR
                     ILANGYPTGVEVCHAMVHGGPFPATSDSRTTSVGTAAIYRFLRPVSYQDIPDTFLPAA
                     LKNANPLGIPRLESK"
     gene            complement(4460..>5187)
                     /locus_tag="VIR04_05510"
     CDS             complement(4460..>5187)
                     /locus_tag="VIR04_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY9460237.1"
                     /translation="KVGLQCGGSDGYSGITANPVLGVATDLLVRQGGTAILSETPEIY
                     GAEHLLTCRAVNPAVGQKLVDLISWWEDRCERNNAAMNNNPSAGNKAGGLTTVLEKSL
                     GGIAKGGSTGLMEVYRYAETVEQSGLVFMNTPGYDPVSATGQVAGGANMICFTTGRGS
                     AYGCAPSPSLKFATNTALWNRQQDDMDMNCGSVIDGDASIEQLGQQLFQLILDCASGQ
                     RSKSELYGYGENEFVPWQLSVIT"
BASE COUNT         1120 a         1560 c         1439 g         1068 t
ORIGIN      
        1 tgatctgggg gctgcagccg ggcccaacct tgtttgtgga acaaaaagac ttcgtctggg
       61 gcctgatcgc cagcatgtat ctgggcaacg ttgtcgggct gattgtcgtg ctgtttaccg
      121 tgccgctatt cgcgtcgatt ctgcgcgtgc cgttttcgat catggcgccg cttatcgtca
      181 tcgtgtgcag catcggcgcc tatgcggtga atcaatcgtt gtttgacgtg ggaatgatgc
      241 tggtattcgg agtattcggc tacctgttca aaaaactggg ttaccccatt gctccgctgg
      301 tgctggcggc tgtgctggga gacaaagcgg aagacgcctt ccgtcagtcg atgctgttct
      361 ctggcggcaa cctgggcatt ttctttcata acggattagc cggcaccctg accatcctgg
      421 ggctggtcat ggcatttctg cctgttatcg gacgcctgtt caagcgtaat aacccaacct
      481 gacgccatca acaaggcggg cgccggcttg cccgttcaca ttaaaggacc cattgttatg
      541 tcagaacaaa aacccactgt cgccctcacg ctgggcgacc cggccgggat cggccccgag
      601 cttatcgcca aactgcttaa tgaacccagt aatcctgagc acgccaatat cgtactcgtc
      661 ggcgacccat ggctttggca ggcggggcaa cgcattgcgg gcgtaacggt caccactcat
      721 gaaatcccct cactggccga cgctcgccgc aatgcctgcg acaagccctt gtttgtggcc
      781 gtcgacagca ttgcggccag ccaggtgcaa gtgggacagt cggccgccac tggcggcgcg
      841 tcggttctga aaattctgga tttgtgcatg gacgccgcaa tcgcgcgcca gatcgacgcc
      901 atctgcttcg cgccactgaa taagcatgcc atgaagcaat ccggcatgcg gtttcaagac
      961 gaattgcatc acttcgccaa ctaccttaag gtggccggct acttctgcga gttcaatacc
     1021 ctggatggtc tgtggacagc tcgtgtgtcc tcgcacgtgc cgttaaaaga tgccgcttcg
     1081 cacgtaagca aagaacgcat cgaagacgca agccgcctga ttttcgattc cctgaaagcc
     1141 gccggcacgg ctcatccgcg tgttgcggtc gccgcgctaa acccgcatgg cggcgatggc
     1201 gggtcatgcg gacgtgaaga gatcgacatc atcgaaccgg ccgtgcggga tctgaaccgg
     1261 cagggatacg acgtcgacgg gccttttccg gccgacacaa tattcatcaa ggcgcgcgaa
     1321 ggcaaatacg atgccgtcgt cacgatgtat cacgaccagg ggcaaatcgc gctcaaattg
     1381 ctgggctttg aaaaaggcgt cacggttcaa gggggattgc ctatccccat cactacgcca
     1441 gcgcatggca ccgcctacga aatagccggc aaaaaccagg ccaccgtcac ggccatgcgc
     1501 aatgcgttct tgatcgcatg ccgcatgggc aacgcttacc ggaaacaagc cgcttgccaa
     1561 accagcggca cacccattta aacggagaat caagtgaaaa tcgtcaacgt ccgcgcacgt
     1621 gttttcgaat ggaaaggcaa aaccacgccg ccgcaggccc acttttgcgc caacgcaatg
     1681 gatttgctgt gggatcgtgg cgattcgatg ggcacattcc ggttccacgg ctggaccgtc
     1741 gtcgagatag aaaccgacga cggcctgaca gggatcggca acgccgccct ggctccccgt
     1801 attgccaaac atattatcga ccagtacctg gcgccgctgg tcactggcca agatcccttc
     1861 gattacgagt atttgtggca acgcatgtat cgctcgacac tggcgtgggg acgcaaggga
     1921 ataacgatgg cggcgatttc agcgattgat atcgccttgt gggatcttat gggcaaagca
     1981 accgggcggc ccgttttcaa actgctgggc ggccgaacca aagaaaaaat cccctgctat
     2041 gcgtccaaac tctatcacaa cgacctgggt gaaatgcagc gcgaggcgca gtcgtacctg
     2101 gatcaggggt tcagcgccat gaaactgcgt ttcggctatg gccccaaaga cgggcctgca
     2161 ggcatgcgca aaaatattga aagtgttgac gccgtgcgcg aggtcatagg gtacgacacg
     2221 gatctgatgc tggagtgcta tatgggctgg aacctggact acgccaaacg tatgctgccc
     2281 aaacttgagc gctttgaacc caggtggctc gaagagcccg tcatcgccga cgatatcgac
     2341 ggttacgccg aacttaacaa gctgaccgcc attcccattt caggcggcga gcatgaattc
     2401 accagttatg gctttcgtca gttgctggac aaacgcgcgg tatccgtgat tcagtacgac
     2461 accaaccggg tcggcggcat taccatggct cacaaaatta acgccctggc cgaagcttac
     2521 agcgtaccgg tcgtgccgca cgcggggcaa atgcataact accatctgac catgtcgacc
     2581 ctggcctcgc ccttgtccga attcttcccg gtgcacgacg tcgaggttgg caacgaactg
     2641 ttttattata ttttcgacgg cgaccccgtc ccgaaaaatg gctaccttga ccttgacgat
     2701 acgctgcccg gtcttggcct gaccctgtcc gacaaatacc tggatcaatt caatatcatt
     2761 gaataagccg gcgtgtgcaa ttggcgcaaa acgggtatgc gatatttatc ttcataaatg
     2821 gtatcgttag gcagaaagca gaccaacccg catatatgta aaggcgctta aatgaaaatc
     2881 agtggaacca tgctaatcgg cgcgaaagac gtcgtcggca caaaaaaaga catcaaggct
     2941 gtcaatcccg cgaccgatga aacgctggaa cccaccttca aggggggcac cagcgcagaa
     3001 gtcgaacagg cctgccaact ggcgcattcc gcgtttcaga gctatcgcga aaccagcctt
     3061 gaacagcgcg cggttttcct ggaaaagatc gccgatcaca tcatggcgct gggcgatgaa
     3121 ctgattgttc gcacgcatct ggaatcgggc ctgcccaagg ggcgaataga aggcgaacgc
     3181 ggccgcacgg tcaaccagtt gcggctgttc gcttcggtgg tgcgcgacgg gcgctggatc
     3241 gacgcgcgca tcgatttcgc ccagcccgag cgcaaaccca tgcctcgcag cgacattcgt
     3301 ctgcgctata ttccactggg accggtcgcc gtttttggcg ccagcaactt cccactggcg
     3361 ttttcagtcg ctggcggcga caccgcgtcc gcattggccg ccggcgcccc cgtcatcgtt
     3421 aaagcgcact cggctcaccc tggaacatcc gaactggtcg gccgcgccat acgtgcggcg
     3481 gtggcagaat gcggcttgca cgaaggcgtt ttctcgttaa ttttcggtgc gggcgccgac
     3541 gtcggccaaa ctctgatcac gcatccccac atcaaagcgg tcgccttcac cggctcgcgc
     3601 acggccgggg tcgctctcat gcatagcgct gccgcacggc ccgagcccat tcccgtctat
     3661 gccgaaatga gcagcatcaa tcccgttttt ctgatgccgg gcgcgctcaa ggccaacgcg
     3721 gaaaaggtcg cgcagggttt cgtggcatcg ctgcttatgg gagcgggtca attttgcacc
     3781 aacccgggcc tggccatcgg gatcgattcc cccgaactgg atgcattcat tcgcgccgct
     3841 gaagcgggcg tggtcgccag tcccgccgcg accatgctga catccaatat tcacaaagcg
     3901 tacgagacga atctgaacca gcttgtactg aacccgaata ttaccgtgct ggcgcgcggc
     3961 aagacttccg acgcgcccaa ccaatgccgt tcggcgctac tgtgcaccag cgccagcgcc
     4021 tttttgaaag atccgcacgg tttggggatc gaagtattcg gctccagctc cgtcgttatc
     4081 aaatgcgcca actccgatga aatgctggca attgttcagg ccctcgaagg ccagcttaca
     4141 gccactctgc aaatagcggc cggccaggac gaagcgctga cgacatcgct tattgccgcg
     4201 cttgaattga aagtcggccg cattctggcc aacggctatc cgacgggcgt cgaggtatgc
     4261 cacgccatgg tgcatggggg gccattcccc gccacatccg acagccgcac gacttcggtg
     4321 ggcacggcgg ccatttatcg ctttctgcgg cccgtgtcgt atcaggacat ccccgacaca
     4381 ttcttgccgg ccgccttgaa aaacgccaat ccgctaggca tccccagact ggaaagcaag
     4441 taaaacgatc gggcagcgct caggtgataa cgcttagttg ccatggcacg aactcatttt
     4501 ccccgtagcc atacagctcg ctcttcgagc gctgacccga ggcgcagtcc aggattaact
     4561 ggaatagttg ttggcccaac tgctcgatac ttgcatcccc atcgatgacc gagccgcaat
     4621 tcatgtccat gtcgtcttgc tggcgattcc acaaagcggt gttggtcgcg aatttcagtg
     4681 acggcgaggg cgcacaaccg taggctgagc cgcgccctgt cgtgaagcag atcatattgg
     4741 ccccgcccgc aacctgtccc gtcgctgaaa cgggatcgta gccgggcgta ttcatgaaca
     4801 ccaggccact ttgctccacc gtttcggcat accgataaac ttccatcaag ccggtcgagc
     4861 cgccttttgc aatgccgccc agcgattttt caagcacggt ggtcaggccg cctgccttgt
     4921 tgcccgctga ggggttgtta ttcattgcgg cgttgttgcg ttcgcagcga tcttcccacc
     4981 acgaaatgag atcaaccagt ttttggccga ccgccggatt gaccgcgcga caagtcagca
     5041 ggtgctcggc tccataaatt tcgggcgttt cggaaagtat ggcagtgccg ccctgacgca
     5101 ccagcaaatc ggtcgccacg cccaaaaccg ggttggctgt aatacccgaa tagccgtctg
     5161 atccgccgca ttgcaagccg accttga
//
LOCUS       DATCXU010000246         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1072730,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000246 DATCXU010000000
VERSION     DATCXU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072730"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VIR04_05515"
     CDS             complement(<1..939)
                     /locus_tag="VIR04_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HEY9460238.1"
                     /translation="MQVLLDRTFERTADRLLRELGVEAYRESQIEIWLFEDTAKRQAL
                     QAGLLALSVHAKVLSAYKPLVHFFLDEVHAEGLQRVRIVYPCHAACVANRFLLEAYPL
                     TDLFPDVDVAFTQAIETSTESYYQVKLVYKDRAEDHRVFAPNVLGQDFLGLNVYAPSA
                     WLKVKNHGQISSTAIQADYQQVYQAVMDVVDKHDWGQTEPYFDRLTVVLNLPGIERKL
                     SCGQEIVSTSEAMHEEVYFSLLEFFQSYSGRKQGSRGLQPGQIVPDIRLDNGGVPHAK
                     VMLEPSTAFRAEAPRVDPVLLNRTPVVGESVRLDDAD"
     gene            complement(942..1811)
                     /locus_tag="VIR04_05520"
     CDS             complement(942..1811)
                     /locus_tag="VIR04_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460239.1"
                     /translation="MEAAKSLPRTSALKRRPHFSIELYVGAFLTIVIVAAVLLSWWLF
                     PGGGETMDLAARLTHPFTSAAHILGTDPLGRDVLARVIVGGRISLTVGILSVLGAVAL
                     GMLIGVVAGYYRGFWEMFFMRCVDVQLALPFILVAITFISILGGSVVNLIIFMILAQW
                     VQYARLVRAQVIELREREFIQAARSFGVSDWAQIWHHFLPNLMGPVIILMALNVANNI
                     LLESSLTFLGLGVDPLIPSWGGMLADGRTYLQTAWWVSVFPGLAIMFTVLGLNLLADW
                     SRDKLDPSGHTGA"
     gene            complement(1811..2734)
                     /locus_tag="VIR04_05525"
     CDS             complement(1811..2734)
                     /locus_tag="VIR04_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460240.1"
                     /translation="MTGFLIKRFFQALFVIVAVTLLVSYAVRLTGDPALMLAQGAGSV
                     TEADLENIRQGLGLNQPFIWQYWDFISGLMVGNFGHSFLGGTPVARLIADALPATLWL
                     TLCSLAISLIVSIPLGVHAAVHRGKWSDQTIRVLSLIGLSFPNFWLGIMLVLLFAITF
                     PLFPPSGMDGVRSVVLPALTMGIILSATTIRLVRTSMLETLSTQYIMVVRSKGLKEWV
                     VLYKHALRNCAIPLITFIGLQFGSLIGGIVVVERVFNWPGLGTLAFDSISGRDYPVLQ
                     ATVTVLAMLIVAANLLIDIAYGLVDPRIRTK"
     gene            complement(2783..4309)
                     /locus_tag="VIR04_05530"
     CDS             complement(2783..4309)
                     /locus_tag="VIR04_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460241.1"
                     /translation="MSRFKFTVLAGCLALSVAASPLALAQGGTLTVAPVAPGDPGSFD
                     PIDTFLVAWGSIGSQIYDGLTMRGADMKLVPGLATSWEMLDNNQRIRFKLRENVKFHD
                     GEPFNAQAVKFTFDRLLGDEGKKGPQRSNYDSIKEIKIVDEHTVDFLLARPDPVLLIK
                     LAGYGSMIVPPKYVTEKGDAYFNAHPVGTGPFKFVSYQPKVSLTLERNPDYWGGAPKL
                     EKLVYRFITEPSTQASELLAGRLDIAANIPLSMASVIEKDSHLNVVAIDGPTTAALRY
                     DTRDGITKDPEVRKALTLAVDRNAIIKQIMLGYAVPAASFQGKLSFGYDENLKPLPFD
                     PAQAKKILKKQGVKPGTPVQIDFRGSDANFREVAQAVAGYFQAVGLKPTLKGYETSVL
                     LNDIIPNGKTGNMWQNLWGGWTFDYDNTAYAMYHSGQKWNPYDKDAKLDAMLEEQRNT
                     YDQDKRLKILREVAAYVQDHALEMPLYNIKAVFGVNKRVKNLTIPSDGRFRFVNATVD
                     "
     gene            complement(4354..>5402)
                     /locus_tag="VIR04_05535"
     CDS             complement(4354..>5402)
                     /locus_tag="VIR04_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460242.1"
                     /translation="VPRLGQMTGQPFPKRTPVVQLLEDGTLQSVGGERVQDTANYTTP
                     VLEARNLSTRFDVAHDFFGRTTYRVHAVEHVSFSLYPGETLALVGESGSGKSTVGKSI
                     QQLVDATEGSILFNGEDIFKMSAARRRDLKREIQYVFQDPFASLDPRKTVEFSIAEPI
                     VTHRILRDRDAIKARVAQLLENVGLKPEHASRFPHEFSGGQRQRICIARALACDPKLI
                     IADESVAALDVSTQAQIIDLLMKLQQERGLAYLFITHDMAVVEKISHRVAVLYLGQLV
                     ELGSRQAVFETPRHSYTRKLLSAVPVATPGRHVDKALVVSEIPSVVRSVKDLPTINPL
                     AEVTSGHWVAREAV"
BASE COUNT         1357 a         1554 c         1382 g         1109 t
ORIGIN      
        1 gtcggcgtca tcaagccgga cagattcccc gacgacaggc gttcggttta acagcacggg
       61 atcaacgcgc ggcgcttcgg cacggaatgc cgttgagggt tcaagcatca ctttggcatg
      121 cggcacgccc ccattgtcca gccgaatatc gggcacaatc tgccccggct gcaagcctcg
      181 actgccttgt tttcgcccgg aataagactg aaaaaattcg agcaaggaaa aataaacctc
      241 ttcgtgcatc gcctcggatg tactgacaat ttcctggccg caagacagct tgcgctcgat
      301 gccgggcaaa tttaacacga cagtcaggcg gtcgaaataa ggttcggtct ggccccagtc
      361 gtgtttatcc accacgtcca tcaccgcctg atagacttgc tgataatcag cctgaatggc
      421 cgtgctcgaa atctgaccgt gatttttcac ttttagccag gccgatgggg cataaacatt
      481 caagcccaga aaatcttgcc ccaaaacatt gggcgcgaac acacgatggt cttcagccct
      541 atccttgtaa accagcttga cctgataata actttcggtt gacgtttcaa tcgcctgagt
      601 aaacgccacg tcaacgtcag gaaaaagatc agtcaaaggg taggcttcca gcaaaaaacg
      661 attcgcgacg cacgcggcat ggcaagggta cacaattcga acccgttgca agccttcggc
      721 gtgaacttcg tccagaaaga aatgcaccag tggcttatac gcgcttagca ctttggcatg
      781 cacgctcagc gcaagcaagc cggcctgaag cgcctgcctt tttgccgtgt cttcaaacag
      841 ccaaatctca atctgagact cacggtacgc ttcgacgccc aattcccgca gcagacgatc
      901 ggcagtgcgt tcgaacgttc ggtcgagtaa aacttgcatg gtcaggcgcc tgtgtgtccg
      961 gatggatcca gcttatcgcg cgaccaatcg gccagcaaat taagacccaa cactgtaaac
     1021 atgatagcga ggcccgggaa tacgcttacc caccaggccg tctgtaaata ggtgcgcccg
     1081 tcggccagca ttccgcccca actcggaatc aagggatcga cccccaggcc caggaacgtc
     1141 aggctgcttt caagcaagat gttgttggca acgttcaacg ccatcagaat aatcacgggg
     1201 cccatcaggt tgggcaagaa atgatgccag atctgggccc aatcgctcac cccaaaagag
     1261 cgtgcagcct gtataaattc gcgctcacgc aactcgatta cctgagcgcg cacgagacgg
     1321 gcgtactgca cccattgcgc cagaatcatg aagatgatca gattgacgac gctgccgccc
     1381 aaaatcgaaa tgaaggtaat ggctaccaaa ataaaaggca aagccaactg cacgtcgacg
     1441 cagcgcatga aaaacatttc ccagaagccg cggtaatacc ctgccaccac gccaatcagc
     1501 atacccagcg caaccgcgcc cagcaccgac aatatgccaa cagtcagcga aatacggccg
     1561 ccgacgatta cacgcgccaa aacatcgcgc cccaaaggat cggtgcccaa aatatgagca
     1621 gccgaagtaa acgggtgggt caggcgcgcc gccagatcca tggtctcgcc gccgccggga
     1681 aacaaccacc aggacagcag caccgcggca acgatgacaa tagtcaggaa agctccaacg
     1741 tacagttcga tgctgaaatg aggccgcctt ttcaacgcgg aggttctggg caatgatttg
     1801 gcggcttcca tcatttagtc ctgatgcgcg gatcgacaag cccgtacgct atatcaatca
     1861 ataaattagc ggcgacaatc agcatcgcca atacggtgac cgtcgcctgc aacacgggat
     1921 agtcgcgccc cgaaattgaa tcaaacgcca atgtccccag gccaggccag ttaaaaaccc
     1981 gctcgaccac gacaatgccg ccgatcaggc tgccgaactg caggccaatg aaggtaatca
     2041 aagggatggc gcaattgcgc agggcgtgtt tgtatagcac aacccattcc ttcagacctt
     2101 tgctgcgcac caccataatg tactgcgtcg acaatgtttc cagcatcgag gtacgcacca
     2161 gccggatagt ggttgccgac aaaatgattc ccatggtcaa agccggcaat acgacacttc
     2221 ttacgccatc catgcctgac ggaggaaaaa gaggaaaggt gatggcaaaa agcagaacca
     2281 gcatgattcc caaccagaaa ttagggaaag acagaccgat caaggacaaa acgcgtatgg
     2341 tttgatcgga ccacttgccc cgatgcacag ccgcatggac ccccaaagga atcgaaacaa
     2401 ttagcgaaat cgcaagagag cacagagtca gccacaaagt ggccggcaag gcatcggcga
     2461 tcaggcgcgc cacaggtgtt ccacccagaa aactgtggcc gaaattccca accatcaagc
     2521 cggaaatgaa atcccaatat tgccagatga acggctgatt taaccccagg ccctggcgaa
     2581 tattttcaag gtccgcctcg gtgacgctac ccgcgccctg cgccagcatc agggccggat
     2641 cgcccgtcaa cctgacagcg taggacacaa gcaaagtcac cgcaacgatc acaaacaagg
     2701 cttgaaaaaa acgcttgatc aaaaaaccgg tcatgatggt cgcattaaat gaactgtttg
     2761 gcgggcggtg acagcggctc catcaatcta ccgttgcgtt cacgaagcgg aaacgcccat
     2821 cggacggaat cgtcaggttc ttgacgcgct tgttaactcc gaaaacagcc ttgatattgt
     2881 agagcggcat ctcaagcgca tgatcttgca catatgcggc cacttcgcgc aaaattttca
     2941 gacgtttgtc ctgatcgtaa gtattgcgtt gttcctcgag catggcgtcc agcttggcgt
     3001 ccttatcgta agggttccat ttttgccccg agtgatacat ggcgtaggcc gtgttgtcgt
     3061 agtcaaacgt ccagccgccc cataaatttt gccacatatt gccggttttg ccgttgggaa
     3121 tgatatcgtt gagcaatacg ctggtttcgt aaccttttag cgtcggtttc aggccgacag
     3181 cctgaaaata gcctgcaacc gcttgcgcca cctcgcgaaa attcgcgtcg ctgccacgaa
     3241 aatcaatctg tacgggcgta ccgggcttaa ctccctgctt ctttagaatt ttcttggcct
     3301 gagccggatc aaacggcaga ggcttcaggt tttcatcgta tccgaacgat agcttgccct
     3361 gaaaactcgc cgccggcacc gcgtaaccca gcataatttg tttgatgatg gcattacggt
     3421 ctacggccag cgtcaacgct ttacgaacct cagggtcctt ggtgatgcca tcgcgtgtgt
     3481 catagcgcaa cgcggcagtg gtcggcccgt caatcgcgac gacgtttaga tgggaatcct
     3541 tttctatgac cgacgccatg ctcaacggaa tgttggcggc gatatcaaga cgcccggcca
     3601 aaagctcgga tgcctgagtc gacggctcgg tgataaagcg gtagacaagc ttttccagct
     3661 ttggcgcgcc gccccaataa tccggattgc gctccagcgt caggctgact ttgggttgat
     3721 agctcacaaa cttgaaagga cctgtgccca ccgggtgcgc gttaaaatag gcatctcctt
     3781 tttccgtcac atatttcggt ggaacaatca tggatccata tccggccaac ttaatcaaca
     3841 ataccgggtc cgggcgcgcc aaaagaaaat caaccgtgtg ttcgtcaacg attttaattt
     3901 ctttgattga gtcgtaattt gatcgttgcg ggcctttctt gccctcgtcg cccagcaaac
     3961 gatcaaaggt gaatttgacc gcctgcgcat taaagggctc gccgtcgtgg aacttgacgt
     4021 tctcgcgcaa tttgaagcgg atgcgctgat tgttatcgag catctcccaa ctcgtggcca
     4081 atccgggcac aagcttcatg tcggcgccgc gcatggtcaa gccgtcgtag atctgacttc
     4141 cgatactgcc ccaagccacc aggaaagtat cgatcgggtc gaagctgcca gggtcgcccg
     4201 gagcaacggg cgccactgtt aacgtgccac cctgggccaa cgccaggggc gaagccgcaa
     4261 cagacagtgc aaggcatcct gccaacacgg tgaatttaaa tcgggacatg gtttcagctc
     4321 ctttaagcga ttaacgtagt tcttaaactg ccttcacaca gcttcgcgcg ccacccaatg
     4381 gccggatgtt acctctgcaa gcgggttgat cgttggaaga tctttcacac tgcgcaccac
     4441 actgggaatc tcactgacga cgagcgcttt atctacatgg cggccgggcg tggccacagg
     4501 caccgcggag agcagcttgc gtgtgtagga atgccgcggc gtttcgaaaa ccgcctgacg
     4561 ggatccaagc tcgaccagtt gtcccaaata caacactgca acgcgatgac tgattttttc
     4621 gaccaccgcc atgtcgtggg tgatgaacaa ataggccagg ccgcgttcct gctgcagctt
     4681 catcaataaa tcaatgattt gcgcttgggt cgatacgtcc agcgcagcca ccgactcgtc
     4741 ggcaatgatc agttttggat cgcaggccag agcgcgcgca atgcaaatcc gctggcgctg
     4801 cccaccggaa aattcatgag gaaatcggga tgcatgttcc ggttttaggc ccacgttctc
     4861 gagcaattgc gcgacccgag ccttgatggc gtcccgatcg cgcaaaatgc gatgcgtcac
     4921 aatcggctcg gcaatgctga attcaacggt cttgcgcgga tcgagcgacg caaacggatc
     4981 ttgaaaaaca tattgaatct cgcgcttcaa gtctcgcctt cgtgcggcgg acatcttgaa
     5041 tatgtcttcg ccattaaaca gaatggagcc ctcagtggcg tcaaccaatt gttggatgga
     5101 ttttcccacg gtggattttc cgctacccga ttcgcccacc agcgccaagg tttcacccgg
     5161 atacaggcta aagctgacat gttccactgc gtggacccga tacgtcgtcc gcccaaaaaa
     5221 atcgtgcgca acatcgaagc gagtcgagag attcctggct tccagcacgg gcgtcgtgta
     5281 gttggctgta tcctgcaccc gttcgccgcc gaccgattgc aacgtgccgt cctcgagcaa
     5341 ttgcactacg ggagtccgtt tcggaaaggg ctggccagtc atttgaccca ggcgaggcac
     5401 cg
//
LOCUS       DATCXU010000247         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_393917,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000247 DATCXU010000000
VERSION     DATCXU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393917"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /gene="rnhB"
                     /locus_tag="VIR04_05540"
     CDS             <1..248
                     /gene="rnhB"
                     /locus_tag="VIR04_05540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799764.1"
                     /note="RNH2; RNase HII; binds manganese; endonuclease
                     which specifically degrades the RNA of RNA-DNA hybrids;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY9460243.1"
                     /translation="CAARAIVKGDASVAAISAASILAKTARDADCLLLHARYPQYAFD
                     QHKGYCTELHLTRLRSYGPCPAHRRSFAPVRRLLILA"
     gene            255..1064
                     /locus_tag="VIR04_05545"
     CDS             255..1064
                     /locus_tag="VIR04_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY9460244.1"
                     /translation="MKHIDSRDNAQFKQLVRLVDGKDAGFGLVLEGIHLCRAWLRHVG
                     RPMQAIFDAARLQTAPELAELAADLPDRLCMSCAPRLARRLSQVENGQGVYFVVKAPA
                     PAVPEYIDHNGLWLDRVQDPGNLGTLLRTAAAAGIRHAYLSSGCASAWSQKTLRSAQG
                     AHFVMAIHEQLDLLAECGKLKVPLIAAALGQAQSLYSEPLPETCVWLLGNEGQGVDAS
                     LLQRADRRVFIPQTEGVESLNVAAAAAICLFEQRRQCLSRIQRDRPNKSGG"
     gene            complement(1045..1878)
                     /locus_tag="VIR04_05550"
     CDS             complement(1045..1878)
                     /locus_tag="VIR04_05550"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY9460245.1"
                     /translation="MTATHTERTVFIVSDSTGITAETFSHSVLSQFDQVKFLPIRIPF
                     IDNAQKADETASRINRCALEEGHPPLVFSTLVNPDIASKIRQSNCTFLDLFGTFVAHI
                     EVALGMKSSHSVGRSHMGGLSKQYNNRIEAINFSLAHDDGQFVQGLKQADVILVGVSR
                     CGKTPTSLYLAMQYAIKAANYPLIPEDFERDSLPSTLLPFRNKLFGLSIRPARLAQVR
                     NERRPNSHYASLEQCRNEVAAAERLMRMENIPCLSTTTKSIEEISTKVIEEVGLNRRT
                     Y"
     gene            2057..>2295
                     /locus_tag="VIR04_05555"
     CDS             2057..>2295
                     /locus_tag="VIR04_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY9460246.1"
                     /translation="MSYVVSFEQLRMTDVDSVGGKNASLGEMISQLAQAGVRVPGGFA
                     TTADAFRGFLRATHLDQRIADRLNGLDSEDIRELAA"
BASE COUNT          467 a          591 c          687 g          550 t
ORIGIN      
        1 cgtgcgccgc tcgcgcgatt gtgaagggcg atgcaagcgt ggcggctatt tctgccgcgt
       61 ccattttggc gaaaaccgcg cgcgatgccg attgtctttt attgcatgcc cgttatccgc
      121 aatatgcttt cgaccagcac aaaggctact gcacggaact gcatctgacg cgtttgcgat
      181 cctacggccc ctgcccagcc catcgccgca gtttcgcgcc ggtcagacgt ctgctgatat
      241 tggcatagtt ttcaatgaag cacatcgact cgcgcgacaa cgcgcagttc aaacagcttg
      301 tgcgcctggt tgatggcaag gacgccggtt ttggtcttgt gcttgagggc atccatttat
      361 gccgggcgtg gctgcggcat gttggccggc ccatgcaggc catattcgac gctgcacgtt
      421 tgcaaaccgc ccccgaattg gccgaacttg ccgccgattt gcctgacaga ctgtgcatga
      481 gctgcgcgcc tcgtcttgcg cgccggctgt ctcaggtcga aaacggtcag ggcgtgtatt
      541 tcgttgtgaa ggcccccgcg ccggctgttc ccgaatacat tgatcataac ggtttgtggc
      601 tggatcgtgt tcaggatccg ggcaatttgg gaacgttgct tcgcacagcc gccgctgcgg
      661 gtatccggca tgcctatttg tcgtcgggct gtgcttcggc ctggtcgcaa aagaccttac
      721 gcagcgctca gggagcgcat tttgtcatgg cgatccatga gcaattggat ttgctggccg
      781 agtgcggcaa actgaaagtt cctttgattg cggcggcatt gggacaggcg caatccttgt
      841 acagcgagcc cttgcccgaa acgtgcgtct ggttgctggg caacgagggg caaggcgtcg
      901 atgccagctt gttgcaacgc gccgacagaa gggtgtttat tcctcagacc gaaggcgttg
      961 aatcactgaa tgtggccgcg gcggctgcca tctgcctgtt tgaacagcgc agacagtgcc
     1021 tgagccgcat tcaacgagat cgccctaata agtccggcgg ttaaggccga cctcttctat
     1081 aaccttggtt gaaatttctt caatggattt ggttgtcgtc gacaggcagg gaatattttc
     1141 catgcgcatc agacgttcgg cggcagccac ttcgttgcga cattgttcca gcgaggcgta
     1201 atggctatta ggtctgcgct cgttgcgtac ttgagccaga cgcgcagggc gaatcgacaa
     1261 accgaataat ttgttgcgaa aaggaagcag cgtcgagggc agcgaatcgc gctcaaagtc
     1321 ttccgggatg agaggataat tggcggcttt aatggcgtat tgcatggcca ggtacagact
     1381 ggttggcgtt ttgccgcagc gtgacacgcc gaccagaatg acatcggcct gttttaagcc
     1441 ctgcacaaat tggccatcgt cgtgagccag gctgaaatta atggcttcga tgcgattgtt
     1501 gtactgtttt gataaccctc ccatgtgcga gcggcctacc gaatggcttg atttcatgcc
     1561 caacgccact tcgatatgcg cgacgaatgt gccgaacagg tccaggaaag tacagtttga
     1621 ctggcgtatt ttcgaggcga tgtccggatt cacaagcgtg ctgaatacaa gcggcggatg
     1681 accttcttcc agtgcgcaac gattaatgcg gctggcggtc tcgtccgctt tctgagcgtt
     1741 atcgataaac gggatgcgaa tgggtaaaaa tttaacctgg tcgaactgcg acaaaaccga
     1801 gtggctgaag gtttccgccg tgatcccggt actgtcggag acgatgaata cggtgcgttc
     1861 agtatgcgtg gcggtcatgg gaagcgatag cgcaggagcg attggaaacg gtacaatggc
     1921 cgattgtaca gtcaccttgt caaaggctgg tttggttagt gcataggttc gggcggcggg
     1981 aaacgattgg ctgtcccgcg gcgggccata cgccataagc aaacaagctt tcttctatcg
     2041 ttttaaaggt gactttatgt cttatgttgt gtctttcgag cagctccgca tgacggatgt
     2101 cgactcggtc ggagggaaaa acgcatcgct cggtgaaatg atcagccagt tggcgcaagc
     2161 cggggttcgt gtgcccggag ggttcgcgac cacagccgac gcttttcgcg gttttttacg
     2221 tgcaacccat cttgaccagc gcattgcgga ccggttaaat ggactggatt ccgaagacat
     2281 acgcgaactg gctgc
//
LOCUS       DATCXU010000248         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_339780,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000248 DATCXU010000000
VERSION     DATCXU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339780"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="pdxA"
                     /locus_tag="VIR04_05560"
     CDS             <1..900
                     /gene="pdxA"
                     /locus_tag="VIR04_05560"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9460247.1"
                     /translation="ATVVYGDQGILNATIKRLGLNQKLSTRTVAEPAQCSFDAGTINV
                     LNRWQALPAELPIGQLSAQAGRGAYEYLCHAIDDAQAGRLRAIVTAPLNKKAMQAGGI
                     DYPGHTEILAERTRTEHYAMMLVNEELRVILVTIHVALAEVSELITLDAELAVLRLAQ
                     KACLQAGVARPRIAVAGLNPHAGENGRFGQEEINVIAPAIEQARAEGIDATGPWPGDT
                     IFMRARRGEFDIVVAQYHDQGLIPVKYLGVDHGINVTIGLPFVRTSVDHGTAFDIAGQ
                     GIADPSSLKASYDLALTMTPGFH"
     gene            929..1936
                     /locus_tag="VIR04_05565"
     CDS             929..1936
                     /locus_tag="VIR04_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY9460248.1"
                     /translation="MKLRFSVDPFLAKLLFAVLLASLLPVYGAGAVMFGWATNIAIAL
                     LFFLHGARLSREAIVAGGSHWRLHLAIFSATFILFPVLGVLAKPAIIPFTTPELYLGI
                     LFLCCLPATVQSAIAFTSIARGNVSAAVCSASASNMMGVFLTPLLAGLVMSGAGTGDV
                     PISFDAVGKIMVQLLLPFIMGQMLRRWIGQWVIRHGRLLKVVDQGSILLVVYTAFSEA
                     VMGGLWSNTPASALVSLVIISLVLLGLVLGITLLAGRLLKFNIEDRITLLFCGSKKSL
                     STGIPIANILFAGHVVGSIVLPLMIFHQVQLIVCAIIARSYSQRPIHIQAIPTASPSP
                     R"
     gene            complement(1917..3296)
                     /locus_tag="VIR04_05570"
     CDS             complement(1917..3296)
                     /locus_tag="VIR04_05570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY9460249.1"
                     /translation="MDNKRIDGDSQLDYLRVPPHSVEAEQSVLGGLLLDNAAWDRISD
                     VLGEDDFYRFDHRIIWQHITRLIALARPADVVTVNESLSSAAKADEAGGLAYLNALAH
                     NTPSAANIRRYAEIVRERSMLRKLVSVADEIAATAYNPQGKEARQLLDEAESKVFQIA
                     QEGSRGTSGFQQIQPLLTQVVERIDELYHREGDSDVTGVPTGFTDLDRMTSGLQAGDL
                     VIIAGRPSMGKTSLAMNVGEYVAIEQGLPVAVFSMEMGAVQLAMRMVGSVGLLDQHRM
                     RTGKLTADDWPRLTHAVQQVQEAQIYIDETPALNSMEVRARSRRLARQCGQLGLIIVD
                     YMQLMSANSSGENRATEISEISRSLKGLAKELSCPLVALSQLNRSLEQRPNKRPVMSD
                     LRESGAIEQDADLILFIYRDEVYNPDSPDKGSAEIIIGKQRNGPIGSVRLTFQAASTR
                     FLNFTSAMG"
     gene            complement(3390..3845)
                     /gene="rplI"
                     /locus_tag="VIR04_05575"
     CDS             complement(3390..3845)
                     /gene="rplI"
                     /locus_tag="VIR04_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY9460250.1"
                     /translation="MQIILLEKVANLGNLGEVVRVRDGYARNYLIPKKIARRATESAL
                     KEFEARRAELEKIQAEKLAAAQAVGEKLAGMSLTVSQKAGVDGRLFGSVTNMDLVAAL
                     AKEGFTSIEKSHIRLPDGSIKATGEYPVRISLHTDVAVDVTVVVRGEMV"
     gene            complement(3863..4135)
                     /gene="rpsR"
                     /locus_tag="VIR04_05580"
     CDS             complement(3863..4135)
                     /gene="rpsR"
                     /locus_tag="VIR04_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY9460251.1"
                     /translation="MAYFKKTKEKRKFTQQNPLFKRRKFCRFTAANVDQIDYKDLDTL
                     RDFIQENGKIIPARLTGTKAHYQRQLDTAIKRARFLALLPYTDNHN"
     gene            complement(4239..4556)
                     /gene="priB"
                     /locus_tag="VIR04_05585"
     CDS             complement(4239..4556)
                     /gene="priB"
                     /locus_tag="VIR04_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY9460252.1"
                     /translation="MNRVSLTATALEVKPLRYTPAGLPAVEMILNHESEVLEAGVVRR
                     IEMVLPAVALGDIALLLADTPMGAALSIQGFLAPARKSSSKLVLHIQQGSRVFSSQAS
                     ALV"
BASE COUNT          974 a         1353 c         1258 g         1042 t
ORIGIN      
        1 gccacagtgg tctatggcga ccagggcatt ctgaacgcga cgatcaaacg gttgggcctg
       61 aaccaaaaat tgagcacgcg caccgtggcg gaaccggcac aatgcagctt cgacgctgga
      121 accatcaacg tgctgaatcg atggcaggcc ttgccagccg aactgccaat aggccaactt
      181 agcgcgcaag ccgggcgcgg cgcctacgaa tacctgtgcc acgccatcga cgacgcgcaa
      241 gccggccgcc tgcgcgccat cgtgacggcg ccgctcaata aaaaagccat gcaggcagga
      301 ggaatcgact atcccggtca caccgagatc ctggccgaac gcacccgaac ggaacactac
      361 gccatgatgc tcgtcaatga agaactaagg gtgatcctgg tcaccattca cgtggctctg
      421 gccgaggtta gcgaactcat caccctcgat gcggagctgg ccgttttgcg tctggcgcaa
      481 aaagcatgtc tgcaagcggg agtggccagg cccagaattg ccgttgcggg tttgaacccg
      541 catgcgggcg aaaacgggcg tttcgggcaa gaagaaatca acgtcatcgc tcctgcgatc
      601 gaacaggcac gggccgaagg cattgacgcg accggccctt ggcccggcga cacgattttc
      661 atgcgggccc gccggggcga attcgacatc gtggtggctc aataccacga ccaagggctg
      721 attcccgtca agtacctggg cgtggatcac ggcatcaatg ttacgattgg actgccattt
      781 gtgcgcacaa gcgtcgacca cggcaccgca ttcgatatcg cgggccaggg tatcgccgac
      841 ccaagctcgc taaaagcctc gtatgacctg gcactgacca tgacgcccgg cttccactaa
      901 atctgccact tcactgcgcc gcagtttcat gaaattacgc ttttccgttg acccgtttct
      961 agccaagctg ctgttcgccg ttcttctggc cagtcttctg ccggtgtacg gcgcgggcgc
     1021 cgttatgttc ggctgggcta ccaacattgc aattgccctg ttatttttct tgcatggagc
     1081 ccggctttcg cgcgaagcta tcgtggcggg ggggtcgcac tggcgtctgc acctcgccat
     1141 tttcagcgcg acgtttattc tgtttcccgt tctgggggtt ctggccaagc cggcgatcat
     1201 tccttttacg acccctgaac tgtacctggg tattttgttt ttatgctgcc tgcctgccac
     1261 cgttcaatcg gccatcgcct tcacgtcaat cgcccgcggc aatgtttcgg cggctgtgtg
     1321 cagcgcatcg gcatccaaca tgatgggcgt atttctcaca ccgctgctgg ccggcctggt
     1381 catgtccgga gcaggcaccg gcgacgttcc gatatcgttt gacgcagtcg gaaaaatcat
     1441 ggtgcagttg ttgcttccgt ttatcatggg acagatgctg cgccgctgga tagggcaatg
     1501 ggtcatcagg cacgggcgcc tgctgaaggt cgtcgaccag gggtccattc tgctggtcgt
     1561 ctacacggcc ttttcggaag ctgtcatggg cggattatgg agcaatacgc cagcgtcggc
     1621 gcttgtctcc ctggtcatta tctcgctggt gctgctggga ctggtgcttg gaatcacctt
     1681 gctggccggc cggcttctga agttcaatat tgaagatcgc atcacacttt tgttttgcgg
     1741 ttccaaaaaa agcctgagca ccggcatccc catcgccaat attctgtttg cgggccatgt
     1801 ggtcggatcc atcgtcttgc cgttgatgat tttccatcag gtgcaactga tcgtttgcgc
     1861 gattattgcc agatcctaca gccagcgccc catccacatt caggctatcc caaccgctag
     1921 cccatcgccg aggtgaaatt caaaaagcgc gtgctggcgg cctggaaagt taaacggacg
     1981 ctgccaatcg ggccattgcg ctgcttgccg ataataattt cagcactgcc cttgtccggc
     2041 gaatcggggt tatagacctc gtcgcgatag atgaacagga tcagatcggc gtcttgttcg
     2101 atagcgccgg attcgcgcag atcgctcatg acgggccgtt tgttagggcg ctgttcaagg
     2161 ctgcgattca gttgcgataa cgccaccagg gggcagctaa gctccttcgc caggcctttc
     2221 agcgaccggc tgatctctga aatttccgtc gcccgatttt caccggaaga attcgccgac
     2281 atcaactgca tgtaatccac aatgatcaga ccaagttgac cacactgacg cgccagacgc
     2341 cgcgaccgtg cgcgcacctc catggagttc aaagccggcg tttcgtcgat gtagatttga
     2401 gcttcctgca cctgttgaac ggcatgcgtc agacgaggcc agtcatcggc agtcagtttg
     2461 ccggtgcgca tgcgatgctg atccagcaga cccaccgaac ccaccatgcg catggccaac
     2521 tgcacagcgc ccatttccat ggaaaatacg gccaccggca aaccctgttc gatcgcgacg
     2581 tattctccga cgttcatggc cagggacgtt tttcccatgg acggccggcc ggcaatgatc
     2641 actaaatcac ctgcctgcag gccggaggtc atgcgatcca gatcggtaaa ccccgttggt
     2701 acgcccgtaa catctgagtc cccctcacga tggtagagct cgtcaatacg ttcaaccact
     2761 tgtgtgagca agggctgaat ttgctgaaaa cccgacgtgc cgcgcgatcc ctcttgtgca
     2821 atctgaaaca ccttggactc agcttcgtcc agaagctgac gtgcctcctt gccctgaggg
     2881 ttatacgcag tcgccgctat ttcgtcggca accgacacaa gtttgcgcaa cattgagcgc
     2941 tcgcgcacaa tttcagcgta acgccggatg tttgccgccg agggcgtgtt gtgcgccaat
     3001 gcattcagat aggccaagcc gcccgcttcg tccgccttcg ccgcgctgga cagggactcg
     3061 ttgaccgtca ctacatcggc gggccgggcc aacgcgatca aacgcgttat atgttgccag
     3121 atgatgcggt ggtcgaaacg gtagaaatcg tcctcgccca gcacatcgct aatccggtcc
     3181 caggcggcat tatccagcag caatccaccc agcactgact gctccgcctc gaccgaatga
     3241 ggcggcacgc gcaagtaatc caactgggaa tcgccatcta ttcttttatt gtccaaatca
     3301 gcaccctgga gccgctatac agaagaaaaa aaagccggta tgcaccggct ttcaatcata
     3361 acaaggtacg gcgtcattgc gatcgcggat cagaccatct cgccacgcac gacaaccgta
     3421 acatccaccg ctacgtcggt gtgcaacgaa atccgaaccg gatactcgcc cgttgccttg
     3481 atggatccat cgggaagccg aatatgggat ttctcaatgg aggtaaagcc ttctttggcc
     3541 agagccgcga ccaggtccat attggtcacc gaaccgaaca gacgcccgtc taccccggct
     3601 ttttgggaca cggtaagcga catgccggcc agcttttcgc caacagcctg ggcggccgcc
     3661 agcttttcag cctggatttt ttccagttcg gcgcgacgcg cttcgaattc cttcagtgcg
     3721 ctctctgttg cgcgccgggc aattttttta gggatcaggt aattgcgagc gtagccgtcg
     3781 cgcacgcgaa cgacttcccc aaggttaccc aggttggcaa ctttttcgag cagaataatt
     3841 tgcatgacga taggccttag ggttagttat ggttgtcggt gtagggcagc aatgccagaa
     3901 aacgagcgcg tttgattgcg gtatcgagct gacgctgata gtgcgcttta gtgccggtta
     3961 aacgcgccgg aattatcttg ccgttttcct ggatgaaatc ccttagcgta tccagatctt
     4021 tgtaatcgat ctgatccaca ttcgcggccg taaaacggca gaacttgcgc cgcttgaaca
     4081 acggattttg ttgagtgaat ttacgttttt ctttcgtttt cttgaaataa gccatgatat
     4141 gcctgtttgg ttcgtgggcc tttcgcccta actgtatcta atatctatta atgaatcgaa
     4201 ctgatcgcac gccgcgaaaa ttttgtctgg agcctttgct aaacaagcgc tgacgcctgg
     4261 ctggaaaaga cccggctacc ttgctgtata tgcagcacca atttgcttga gcttttccga
     4321 gccggagcca aaaagccctg gatcgaaagc gctgcgccca ttggggtatc tgccagcaac
     4381 aaagcgatgt ctcccagggc aacagcagga agcaccattt cgattcggcg aacaacgccg
     4441 gcttcgagga cttccgattc gtgattcagg atcatttcaa cagcaggcag accggccggg
     4501 gtatagcgca ggggcttgac ctcgagcgcc gttgccgtca aagaaacacg attcacgcct
     4561 gtgcctggaa cacctggcaa ttattcctcg tgcgtggcga ccggagctgg agttgcaacc
     4621 ggggttt
//
LOCUS       DATCXU010000249         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_3560, whole
            genome shotgun sequence.
ACCESSION   DATCXU010000249 DATCXU010000000
VERSION     DATCXU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3560"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..619
                     /locus_tag="VIR04_05590"
     CDS             155..619
                     /locus_tag="VIR04_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460253.1"
                     /translation="MFGRMAVAACCMAALTACGTTGHSFDSSAIELIVPGQTTLDEAS
                     TLLGSEPVNVYRQVNGAATARWAEKATLVTDAVYFRQELWLAFGPDGRFERVVKKVNV
                     PGSIRRSAPRARVTTSGSSNAGATSSSPGPANSPALAPGLIDSGAVTTYPLK"
     gene            complement(625..1434)
                     /gene="fliR"
                     /locus_tag="VIR04_05595"
     CDS             complement(625..1434)
                     /gene="fliR"
                     /locus_tag="VIR04_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY9460254.1"
                     /translation="MISVEISQLYAWLTAFIWPFFRILALVSTAPLLGESSIPTRVKI
                     GFAALIALAIAPSLAPMPAIAPASFSGLWITAQQILIGIALGLTMRIAFAAVQTAGEF
                     IGLQMGLSFASFFDPGTGANTAVLSRLMNLIAMLMFLALNGHLLMLAGLIRTFDYLPV
                     GIGALNPRGWEALFDWSSEIMLSGMLLALPLIIVLLTINLALGILNRTAQQLSVFAVG
                     FPISLMTGIILLAIVLPQTSPFLANLFQSGYEAMGKVTAGLAGSVDLMPLR"
     gene            complement(1456..1725)
                     /gene="fliQ"
                     /locus_tag="VIR04_05600"
     CDS             complement(1456..1725)
                     /gene="fliQ"
                     /locus_tag="VIR04_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY9460255.1"
                     /translation="MTSETVMSMTYLAMKVALIMVGPVLLATLVVGLIISIFQAATQI
                     NEMTLSFIPKLLAAGITVVLLGPWLISTMVDYIKLLFSQIPGLVS"
     gene            complement(1735..2451)
                     /gene="fliP"
                     /locus_tag="VIR04_05605"
     CDS             complement(1735..2451)
                     /gene="fliP"
                     /locus_tag="VIR04_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930334.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY9460256.1"
                     /translation="MFCALHPVLTLAQIALPAITSTTGTNGNQTYSLSIQTLLLLTMM
                     SFLPAILLMMTGFTRIIIVLGLLRNALGSGMSPPNSVLVGLALFLTFFAMSPVFDQMY
                     TQAYQPLSAGAITFETAVDRGVEPLKTFMLHQTREADLSLFAGMAKIGELQTRADIPL
                     RVLVPAFVASELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPVTISLPFKLML
                     FVLADGWHLLLGSLARSFYQ"
     gene            complement(2548..2889)
                     /gene="fliO"
                     /locus_tag="VIR04_05610"
     CDS             complement(2548..2889)
                     /gene="fliO"
                     /locus_tag="VIR04_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY9460257.1"
                     /translation="MTESAVLRLVLSLFLIIALILAGAWLTRRAGWLRGTANRSIKVI
                     GSQSLGPRAYVALVEVENTRLVLGVTATQISLLHTLPEAGRDQHGSMPAASEPTPIFA
                     GSLKKIMALRR"
     gene            complement(2897..>3041)
                     /locus_tag="VIR04_05615"
     CDS             complement(2897..>3041)
                     /locus_tag="VIR04_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY9460258.1"
                     /translation="GGEPMDIFVNGYLIAQGEVVVVDQKYGIRLTDIITPSDRLSRLN
                     SRR"
BASE COUNT          733 a          827 c          870 g          611 t
ORIGIN      
        1 tgacggtggc agccaagcct gccgcgatcc cgtccgcgcc ccatcgtccg ttggcggccg
       61 atcagaagac gcccgctgct tatgccgctg cggcaaataa cgacgatgag tgggtggaat
      121 tttaacgatg ttcatggccg cgcgctcaag gcttatgttt ggccgtatgg cggtggcggc
      181 ctgttgcatg gcggcgctga cggcatgcgg cacaacgggt cattcgttcg attcatccgc
      241 gatcgaactg atcgtgcccg ggcaaaccac gctggatgaa gccagcaccc ttttgggttc
      301 cgaaccggtc aatgtctatc gccaggtgaa tggcgcggca acggcgcgct gggctgaaaa
      361 agcgacgctg gtcaccgatg cggtttactt tcgacaagaa ttgtggctgg catttggacc
      421 cgatggccgg tttgagcggg tcgtaaaaaa ggtgaatgtg ccgggttcga tcaggcggtc
      481 cgcgcctcgg gccagagtca ctacaagcgg atcttccaat gcgggtgcta cttcatcttc
      541 gccgggcccg gctaattcgc ctgcattggc gcccggcctg atcgatagcg gtgcggtgac
      601 gacatatccg ttgaaataat acggttaccg caatggcatc aaatccacgc tgccagccag
      661 tcctgccgtt acttttccca tcgcctcgta ccccgactga aacaggttcg ccagaaatgg
      721 gctggtctgc ggcagaacga tcgccagaag gattattccc gtcatcaggc tgatagggaa
      781 gcccaccgcg aacaccgaca actgctgggc cgttctgttt aaaatcccaa gcgccaggtt
      841 aatcgtcagc aaaacgataa tcagcggtag cgcaagcaac attcctgaca acatgatctc
      901 cgaactccag tcaaacagcg cttcccagcc cctcggattc agggcgccga tcccaaccgg
      961 caagtaatcg aacgtgcgga tcagtccggc cagcataagc agatgaccgt tcaatgccag
     1021 gaacatcagc atcgcgatca aattcatcag acgagacagc acggcggtat tggctccggt
     1081 tcctggatcg aagaaggacg caaacgacag gcccatttgc agtccgatga attccccggc
     1141 ggtttgcacg gcggcaaacg caatgcgcat ggtcagccct agcgcgatgc cgatcaatat
     1201 ttgctgggcg gtgatccaga gcccgctaaa cgatgccgga gcgatggcgg gcatgggtgc
     1261 cagagatggc gcaatcgcca atgcaatgag ggcggcgaat ccgattttga ctcgggtggg
     1321 gattgacgat tcgcccagca acggggcagt actcaccagc gccagtattc ggaaaaaagg
     1381 ccagatgaac gcggtgagcc aagcgtacaa ctggctgatc tcgacagaaa tcattcgtct
     1441 gggctttact ctgacttacg aaaccagccc gggaatctgg gaaaacagca gttttatata
     1501 gtcgaccatc gtgctgatca gccatggacc caacaatacg acggtgattc ccgcggccaa
     1561 tagcttcgga atgaatgaca aggtcatttc attgatttgc gtggcagcct ggaaaatgct
     1621 gatgatcaac cctacaacca gggttgcgag cagtaccggc cccaccatga tcagggctac
     1681 tttcatggcc aggtaggtca tggacataac ggtttccgag gtcatttaaa ttccctattg
     1741 ataaaagctg cgcgccaacg atcccagcag taaatgccac ccgtcggcca atacgaacaa
     1801 catcagcttg aaaggcaaag aaatggttac gggaggaacc atcatcatcc ccagagccat
     1861 cagcacgctg gcgatcacca gatcgataat caggaagggg ataaagatgg tgaaaccgat
     1921 ttgaaacgct gttttcagtt cgctggcgac aaacgccgga accagcaccc gaagcgggat
     1981 gtcagcgcga gtttgcagtt ctccgatctt tgccatgccg gcaaacaagg aaagatcggc
     2041 ttcgcgggtt tgatgcagca tgaacgtttt caacggctcc acccctcggt cgacggccgt
     2101 ttcgaacgtg atggcaccgg cgctgagcgg ttgataggct tgcgtgtaca tttgatcgaa
     2161 aacgggcgac atggcgaaaa aagtcaaaaa tagcgccaag ccgaccaaca ccgaatttgg
     2221 cggagacatg cccgaaccga gagcattgcg cagcaggccc agtacgataa tgatgcgggt
     2281 aaaaccggtc atcataagta gaatggccgg cagaaatgac atcattgtca gcaataacaa
     2341 tgtctgtatg ctaagcgagt aggtctgatt gccgttggtt cctgtcgtcg atgtgatggc
     2401 gggcagggcg atttgcgcca gtgtcagtac cgggtgcagg gcgcaaaaca tcgccagggc
     2461 cgacagaatt ccgatggcga gcggcggccg ttttgaattg cggcggcaac gctggaaatt
     2521 cgttttgttc aagaggctgc gcatttttta gcggcgcaat gccattattt ttttcaatga
     2581 gcccgcgaaa atcggcgtcg gttcagacgc cgctggcatg gagccgtgct ggtcccgccc
     2641 ggcttcgggc aaggtgtgca acagtgaaat ctgggtcgcg gtgacaccta gaaccagtcg
     2701 tgtgttttcg acttcgacca gcgcgacata tgcgcgtggc cccaggcttt gcgagccaat
     2761 aaccttgatc gaccggttcg cagtgccacg caaccagccg gcccggcgag ttaaccaggc
     2821 gcccgcaagg atcagcgcaa taataagaaa cagactaagc accaggcgca gaacagcgga
     2881 ttcagtcatg ggagattcag cgcctgctat ttaaccggct aaggcgatcg gatggcgtaa
     2941 tgatgtcggt tagccgtatg ccgtattttt gatcgacaac gaccacttcg ccctgtgcga
     3001 taaggtagcc gttaacgaaa atgtccattg gctccccccc c
//
LOCUS       DATCXU010000250         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_210359,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000250 DATCXU010000000
VERSION     DATCXU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210359"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..850
                     /locus_tag="VIR04_05620"
     CDS             128..850
                     /locus_tag="VIR04_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460259.1"
                     /translation="MFTVASATPPQSGCTNTPRQNRFTRASVVSRAAAVAFAALLLSA
                     CAQQKAPGYYAPEHDTTAADARDQALGRDRAQVPGQIHFGFGNSQKSKPANDTAAAEA
                     PAAAVPATPRALTEAKTFLGTVPCTRNTDACSANRITLTLAPTGEWRARTTILSTRPG
                     AKTATAEQGCWSVIGTEPLRIVLRLKNNNTKASLTFLSDNVLRINRLDGVASNLDYRL
                     TRQADIDPINEIGKRARLQCAR"
     gene            complement(847..1074)
                     /locus_tag="VIR04_05625"
     CDS             complement(847..1074)
                     /locus_tag="VIR04_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY9460260.1"
                     /translation="MSGVFDHEHALFSELTPRERDVMDYVALGKPNKIIAAELGVSQR
                     TIEAHRARIFQKMQVKNAVELARCILSRQFS"
     gene            complement(1191..2033)
                     /gene="panC"
                     /locus_tag="VIR04_05630"
     CDS             complement(1191..2033)
                     /gene="panC"
                     /locus_tag="VIR04_05630"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY9460261.1"
                     /translation="MKVVHTVEELRDQLRGQLRVAFVPTMGNLHQAHLSLMKLARQHG
                     DPVVASIFVNRLQFGPNEDFDRYPRTLQSDIEKLERERDVYVLFAPDEREMYPEPQNY
                     QVQPPAGLGDILEGHFRPGFFAGVCTVVMKLFSCVQPSVAVFGKKDYQQLMIVRNMCR
                     QFQLPVTILAHETVRSPDGLALSSRNIFLDPAERSEAPALFATLNALKQKIKAGRTDQ
                     AELEAGAQAQLTGRGWVVDYVSLRRQRDLSSPTLEEIRDGEPLVVLAAAKLGKTRLID
                     NLEI"
     gene            complement(2052..2930)
                     /locus_tag="VIR04_05635"
     CDS             complement(2052..2930)
                     /locus_tag="VIR04_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY9460262.1"
                     /translation="MPLSGVSAGQLDALIKPAIDSTPDMVDSVALARLYGEPLFAMPR
                     DLYIPPDALEIFLETFQGPLDLLLYLIRKQNFNVLDIPMAELTRQYLSYVDQIRNQNL
                     ELAGEYLLMAALLIEIKSRMLLPVKKSDTGDEVGDPRAELVRRLLEYEQMKLAGQKLD
                     ALPQVGRDFQRAHAFLNLEVGQVLPEISVDDLRRAWADIMRRAQLNATHRVTREQLSV
                     REHMSLILRRLGGVRFIEFTELFMERVLEGDLAAVLVVHFLAMLELARESLLDITQAG
                     PYAPIYVRLSYTRANA"
     gene            complement(2940..3128)
                     /locus_tag="VIR04_05640"
     CDS             complement(2940..3128)
                     /locus_tag="VIR04_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEY9460263.1"
                     /translation="MAKNYESEITQFLNKYKVEHPGTEQRQREGRGRLSDRQLDAELL
                     EGFRVGRVPQPAYVYYSY"
     gene            complement(3215..4042)
                     /locus_tag="VIR04_05645"
     CDS             complement(3215..4042)
                     /locus_tag="VIR04_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY9460264.1"
                     /translation="MPGEIPSSLPLTGADQPSLIRRRALGDFLRSARARVTPDMVGLP
                     QGIRRRTPGLRREELAQLCGISVTWYTWIEQGRNVAVSASVWARLAGVLRLARAERHY
                     LFEMAECADPEHAHYQLNDLPRGLADCVHSITAPAYILDRCWNVLARNVALLHLFDGW
                     PDRSKSPNLLRYIFQDPAARDLVVGWEQRARRVVAEFRADAGPHADEPDVRAVLDDLL
                     KTSPSFAHWWVRQTVVEREGGRREFKHPEHGNVAYRQITFRLATRPDCKLVMLLTES"
     gene            4142..>4409
                     /locus_tag="VIR04_05650"
     CDS             4142..>4409
                     /locus_tag="VIR04_05650"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY9460265.1"
                     /translation="MIPNTLTIAGVDPSGGAGILADIKTMSALGAYGMAVVAALTAQN
                     TQAVTAISRIPADFVAAQIDTIFGDIQVDAVKIGMLGQKDVIRVV"
BASE COUNT         1034 a         1327 c         1181 g          867 t
ORIGIN      
        1 tgaaatcgcc cagttcgaat aaaaaatcaa gcggcatggc caccccctcg tcgacggtaa
       61 ataaggtttt acgacccgtg caattaatgc gtagaatgcc tattgcttta attaatggat
      121 acttgtcatg tttaccgtcg cctcagctac gccgccgcaa tcaggctgca cgaacacgcc
      181 gcgccaaaac cgttttactc gcgcaagcgt tgtttcacgc gccgccgctg ttgccttcgc
      241 agcgctattg ctgagcgctt gcgcgcaaca gaaagcgccg ggatactacg cccccgaaca
      301 tgacaccaca gcggccgatg cgcgcgacca ggccctggga cgcgatcggg cgcaagtgcc
      361 ggggcagatt cacttcggct ttggcaacag ccaaaaatcc aaaccagcca acgacactgc
      421 cgcagccgaa gcgcccgctg cggcagtgcc ggcaactcca cgcgctttaa ccgaggcaaa
      481 aaccttcctg ggcactgttc cttgcacgcg aaacacagac gcgtgctcgg caaatcgaat
      541 aacgcttacg ctggcgccaa ccggcgaatg gcgtgctcgc acaacgattt tgagcaccag
      601 gccgggcgcc aaaaccgcaa cggccgaaca aggatgctgg agcgtgatcg gaaccgagcc
      661 cttgcgaatt gtgttgcggc tgaagaacaa caacaccaag gcaagcctga cattcttaag
      721 cgacaacgtg ctgcgcatca acagactgga cggcgttgcg tccaatcttg attaccgtct
      781 gacgcgccag gccgatattg acccaatcaa cgagattggc aagcgggcgc gcttgcagtg
      841 cgcgcgttag gagaactgcc gcgacaagat gcaacgcgcc agttcgacgg cattcttgac
      901 ctgcattttt tgaaatattc gagcgcggtg cgcctcgatg gtgcgctgcg aaacgcctag
      961 ctcggccgct attattttat ttggcttgcc taacgcgaca tagtccatga catcgcgctc
     1021 gcgcggggtc aattctgaaa atagtgcatg ctcgtgatcg aatacaccac tcattggggc
     1081 ctccgttagt tatccgtcaa atgttcgtca acagattctg tcataggcag ttacaggcgt
     1141 cgcctgtcac ttcttacaga cgcgtcattt cgatcctgcc ctcgctcttt tcagatctcc
     1201 aggttgtcaa tgagccgcgt ttttcccaac ttggcggcag ccagaaccac cagcggttca
     1261 ccgtctctga tttcctcaag agtcggtgaa ctcaagtcgc gctgacgacg aagcgacaca
     1321 taatcaacca cccagccgcg cccggtcaac tgtgcttgcg cacctgcctc gagttcagcc
     1381 tggtcagtgc ggcccgcctt gattttttgc ttgagggcgt tcagggtcgc gaacagggcc
     1441 ggcgcctcgg atcgctcagc cggatccaga aatatattgc gcgaagacaa agccaggccg
     1501 tcggggctgc gtacagtttc gtgcgccagg atggtaaccg gtaactgaaa ctggcggcac
     1561 atattgcgca caatcatcaa ttgctgatag tcctttttgc cgaacactgc cacgctgggc
     1621 tggacacagg aaaacagctt catgaccaca gtgcaaacgc cggcgaaaaa cccggggcga
     1681 aaatgtcctt caaggatatc tcccagtccg gccggcggct gaacctggta attttgcggc
     1741 tctggataca tctcgcgctc gtcaggcgcg aacagcacgt aaacatcgcg ttcgcgttcc
     1801 agcttctcaa tatcggattg caaggtgcgc ggatagcggt cgaaatcctc gttgggtcca
     1861 aattgcaggc gattcacaaa gatgctcgcc acaacgggat ccccgtgctg acgcgccagc
     1921 ttcataagcg acaaatgagc ctgatgcagg tttcccatgg tgggaacgaa cgcaacgcgc
     1981 aactgtccgc gcaactggtc gcgcaattct tcaaccgtat gaacaacttt cactatgcgc
     2041 tccagcgcgt ttcaggcatt ggcgcgagta taagacaggc gcacgtaaat gggcgcatac
     2101 ggcccggctt gagtaatgtc aagaagcgat tcccgggcca gttccagcat cgccagaaaa
     2161 tgaaccacca gcacagcggc caggtcgccc tcgagcacgc gctccatgaa caattcggta
     2221 aattcaataa accgcacacc acccaaacgg cgcaaaatca agctcatgtg ctcgcgtacg
     2281 gacaattgct cgcgcgtaac gcgatgggtg gcgttcaact gcgcgcgccg cataatgtcg
     2341 gcccaggcgc gccgcagatc gtccacactg atctcgggca gcacctggcc aacttccagg
     2401 ttcagaaaag catgcgcacg ttgaaagtcg cggcctactt gcggcaaagc gtcaagtttt
     2461 tgccccgcca gcttcatttg ctcgtattcg agcagacgcc ttaccagttc tgcgcgagga
     2521 tcgcccacct catcgcccgt gtccgacttc ttgactggca aaagcatacg ggacttgatt
     2581 tcgataagaa gcgccgccat cagcaaatat tcgcccgcca gctccaggtt ctgatttcgt
     2641 atttggtcga catacgacag atactgccgg gtcaattcgg ccatcgggat gtcaagcacg
     2701 ttgaaatttt gcttgcgaat caagtacaac aacagatcca gcggcccctg aaaggtttca
     2761 aggaagatct cgagcgcatc gggtggaatg tacaaatcgc gcggcatcgc gaataacggc
     2821 tcgccgtaaa gacgcgccag ggccacgcta tcgaccatat cgggcgtact gtctatggcg
     2881 ggcttgatca gggcatccaa ctggcccgcc gaaacgccag acaaaggcat tggtggccgt
     2941 tagtaagaat agtagacgta agccggctgc ggaacacggc ccacccggaa accttccaga
     3001 agctctgcat caagttgcct gtcggacaga cgcccgcgcc cttcgcgctg ccgttgttca
     3061 gtgcccggat gttcaacctt gtatttattc agaaactggg taatttccga ttcgtagttt
     3121 ttagccataa cgcacatcta atttaagtgg tgaacgtcaa gtttacttca cttgaaccca
     3181 gttagaatcc aaaaaaagca tgatggcgcc tgacttacga ttccgtcagc aacatgacca
     3241 gcttgcaatc gggacgcgtc gccaggcgaa acgtaatttg ccgatacgcc acattgccgt
     3301 gttcggggtg cttgaattcg cgccgcccgc cttcgcgctc gaccactgtc tggcgaaccc
     3361 accaatgggc gaaggagggg ctggttttca ataaatcgtc aagcaccgca cgcacatccg
     3421 gctcgtcggc atgagggccc gcatcggcgc gaaactccgc cacaacgcgg cgcgcacgct
     3481 gctcccagcc aacgaccaga tcgcgcgcgg cgggatcctg gaaaatgtaa cgcaataaat
     3541 tggggctttt gctgcggtcg ggccaaccat caaataaatg cagcaaagcc acattgcggg
     3601 caagcacatt ccagcaccga tccagaatat acgcgggggc ggtaatgcta tggacgcaat
     3661 cggccagccc gcgcggcaaa tcgttaagtt gataatgggc gtgttccggg tcagcgcatt
     3721 cggccatttc aaacagataa tggcgttcgg cccgcgccag acgcagcacg cccgccaaac
     3781 gcgcccatac actggccgac acggcaacgt ttcggccctg ctcaatccac gtgtaccacg
     3841 tgacgctaat gccgcaaagc tgggcaagtt cctcgcgtcg cagtccggga gtgcggcgcc
     3901 gtatgccttg aggcaaaccc accatgtcgg gcgtcacgcg ggcccgggcg ctgcgcagaa
     3961 aatcgcccag cgccctgcga cggatcagac tgggctggtc cgcgccggtt aatggcaaag
     4021 aggagggaat ctcgccaggc atgcatgcgc cctatagtcg acttgagtga aatgcgtagc
     4081 tgtgtcagta tttgcatcgg tccgccaaca gattaaccca agaccaagtc aattgaacac
     4141 catgattcca aatacactga ccatcgccgg cgtggaccct tccggcggag ccggaattct
     4201 tgccgacata aagacgatga gcgccctcgg ggcctacggc atggccgtcg tcgcggctct
     4261 gacagcgcaa aatacccagg ccgtcacggc catttcgcgc attccggccg acttcgtcgc
     4321 ggcgcaaatc gacacaatat tcggcgacat acaagtcgac gccgtcaaga tcggcatgct
     4381 tggacaaaaa gacgtcatac gcgtcgtcg
//
LOCUS       DATCXU010000251         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_381293,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000251 DATCXU010000000
VERSION     DATCXU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381293"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VIR04_05655"
     CDS             complement(<1..482)
                     /locus_tag="VIR04_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stilbene synthase"
                     /protein_id="HEY9460266.1"
                     /translation="MFITALGTAAPEQRYTKLDCLRAFETSDFSARLGARSRRVVETV
                     LTSDNGMDARHLAVDSLKDVFDIDPDTLHARFLHAAPALAHRAAVKALKQAGLACAQI
                     DAVVVSTCTGYLCPGLSGYVIEKLGLRSGVQAFDLVGQGCAAALPNLQLANALLASGQ
                     A"
     gene            complement(505..1164)
                     /locus_tag="VIR04_05660"
     CDS             complement(505..1164)
                     /locus_tag="VIR04_05660"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY9460267.1"
                     /translation="MVQQRVRVKICGLTQIDDVDTSVQAGADAIGLVFYAKSARAVNL
                     ARARELRDAVPAFVDVVALFVNAPHAEVLDVIDQVDPDLLQFHGDESPGYCASFGRRY
                     IRAFRLGAPALASSDAVLENVRAYSDAAGWLFDSYSSGYGGSGLAFDAGLLRGVQSAK
                     DGRPLILSGGLAPQTVAAAVETLRPYAVDVSSGVEASPGIKSAEKIKAFIAAVRSTQR
                     L"
     gene            complement(1237..2037)
                     /gene="truA"
                     /locus_tag="VIR04_05665"
     CDS             complement(1237..2037)
                     /gene="truA"
                     /locus_tag="VIR04_05665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY9460268.1"
                     /translation="MPRMALGVAYDGTHWEGWQTQPHRCTVQDTFEAALRQFTAQPVR
                     TTCAGRTDTGVHSLAQVIHFDVSAERRLESWVRGVNALLPDSIAVQWACAVPDAFHAR
                     FSALSRTYVYVLRTSRARSPITHARVGWVHYSLDLQSMRQAALLLVGEHDFSAFRSSQ
                     CQAASPIRTLHSLQIEQRGEFFIFTFKANAFLHHMVRNLMGVILYVGRGRNPPQWVAD
                     LLAERDRRLAAPTFSPVGLYLAQVEYPSEFSLPMATLNQTLASHLILF"
     gene            complement(2043..>2366)
                     /locus_tag="VIR04_05670"
     CDS             complement(2043..>2366)
                     /locus_tag="VIR04_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460269.1"
                     /translation="AAKKGAAPDVAAKVVPDVAPLVARQGGQAMSWFQEHLLGLITGL
                     LALIVLLIAWALRRANGAHGDDRGNGGLLVTEAMVKEKLDKINLDLHQDAPADKRIEP
                     EIKDV"
BASE COUNT          574 a          729 c          636 g          427 t
ORIGIN      
        1 gcctgacccg aagccaataa agcgttcgcc aactgaagat tcggcaaggc tgcggcacag
       61 ccctggccga ccagatcgaa cgcctgcaca ccggaacgca ggccgagctt ttcgatgaca
      121 taaccgctta agcccgggca caaatagccg gtgcatgtgc tgacgacgac ggcgtctatc
      181 tgcgcgcaag ccaggcccgc ctgtttcaga gccttgaccg cagcccggtg cgccaatgcc
      241 ggtgcggcgt gaaggaaccg cgcatgtaaa gtatccgggt caatatcgaa cacatccttc
      301 agcgagtcaa cggccagatg gcgggcatcc attccgttgt cgctggtcag gaccgtttcg
      361 accaccctgc gcgatcgcgc tccaagacgt gcggaaaagt ccgacgtctc gaaggcccgc
      421 aggcagtcca gctttgtata acgttgttca ggcgccgcag ttcccaacgc agttatgaac
      481 atgtgttcct cgtcgcgaca gaccctacag acgttgtgtc gaccgcaccg cagcgataaa
      541 cgccttgatt ttttctgctg atttaatccc gggactggct tccacgccgc tgctgacatc
      601 aaccgcatag ggacgcaggg tttcaaccgc ggcggcaacg gtctgcggcg caaggccgcc
      661 cgacaaaatc aggggccggc catcctttgc cgattgcacg ccgcgcaaaa ggccggcatc
      721 gaacgccaaa ccactgccgc cgtagccaga actatagctg tcaaacaacc atcccgcggc
      781 atcggaatat gccctgacat tctcgagcac ggcatcggaa gaagcaagag cgggggctcc
      841 cagtctgaag gctcgtatat aacgacgccc gaaactcgcg caataaccgg gcgattcatc
      901 gccgtggaac tgcaaaagat cagggtcgac ctggtcgatg acgtccagca cttcggcgtg
      961 cggcgcatta acgaataaag ccaccacatc tacaaacgcg ggaaccgcat cgcgtaattc
     1021 cctggcccgc gccaaattca ccgcgcgagc gctcttggca taaaacacca ggccaatcgc
     1081 atccgcaccg gcctgaaccg atgtatcgac atcatcgatt tgagttagtc cgcaaatttt
     1141 gactcgaacg cgttgctgca caataaactt ccggcaatga gtggcaacca ctggtaagcg
     1201 gcgattggat ccattgtaat agcgcgcgcg ccagtatcaa aacaagatca aatgcgaagc
     1261 caaggtctga ttcagcgttg ccatcggaag agaaaactcc gacgggtatt ccacttgcgc
     1321 caaatacaag ccaacgggag aaaacgtcgg ggccgccaag cggcggtcgc gttcagccag
     1381 cagatcagcc acccactgtg gcgggttacg cccccggccg acatataaaa tgacccccat
     1441 taaattgcgg accatatggt gcaaaaaggc attggctttg aacgtgaaaa taaaaaactc
     1501 gccgcgttgc tcgatctgca acgaatgcaa ggtgcgtatg gggctggcag cctgacattg
     1561 cgatgatcga aacgcactga aatcatgctc gccgaccaaa agaagcgctg cctgacgcat
     1621 cgactgcaga tcaagtgagt agtgaaccca accaactctg gcgtgggtga tgggcgaccg
     1681 ggcgcgcgag gttcgcagca cgtagacata ggtgcgcgat aacgccgaaa atcgcgcatg
     1741 aaaggcgtcc ggaacggcgc acgcccattg cacggcaata ctatcgggca ataaggcatt
     1801 aacaccgcgc acccacgatt caagccgcct ttctgctgac acatcgaaat gaatgacctg
     1861 cgccaagctg tgcacgccgg tgtccgtacg gcccgcgcag gtagtgcgca cgggttgcgc
     1921 cgtaaattgg cgcaaggccg cttcaaacgt atcctgaacc gtgcaacgat gtggctgcgt
     1981 ctgccagcct tcccagtgcg tcccatcata agcaacgccc aacgccatgc gaggcatgat
     2041 cgtcagacgt cctttatttc aggttcaatg cgcttgtcgg cgggagcgtc ctgatgcaaa
     2101 tcaaggttaa tcttgtcgag cttttctttg accatagcct cggtcacaag gagtccgcca
     2161 ttgccgcgat catcgccatg tgccccattg gcccggcgca acgcccaggc aatcaacaag
     2221 acgatcagag caagcaagcc cgtgatcagg cccagcaaat gttcctggaa ccacgacata
     2281 gcctgccctc cctgcctggc aaccagtggc gccacatcag gcactacctt cgctgctacg
     2341 tctggagcag cacccttttt tgccgc
//
LOCUS       DATCXU010000252         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_610794,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000252 DATCXU010000000
VERSION     DATCXU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610794"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VIR04_05675"
     CDS             complement(<1..418)
                     /locus_tag="VIR04_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460270.1"
                     /translation="MNRLRQLATHPLVVLAAVFIVMPYLMSAAGSTVSLATLVVIYTL
                     YGIAYNLLLGYTGMVSFGSSVFFGMGSYASALFAIHVVQNVALGLIVSTAFGAALGLV
                     LGLLILRRRGLYFALLTLAFTQLFYEICFRWTSLTGG"
     gene            complement(415..1293)
                     /locus_tag="VIR04_05680"
     CDS             complement(415..1293)
                     /locus_tag="VIR04_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460271.1"
                     /translation="MINATLFASQLMNGLVLGFLFVLISVGMSIIFGMLGLVNFAHGA
                     FFALGAYIALSLRPHVGFAGALFLAPILVGAIGLVVERILLRPLYNKEPLLSLLLTFG
                     LALFIEELIRTLWGAMGHPFDTPAFLAGIVHYGPVFVTRYRVAVLVITVALLAAVWLF
                     LEKTPYGRIVRAGSRDPEMVSMLGINLKRVFMFIFGLGCLLAGAAGVLAAPLWSVVPS
                     MAEQAIMPAFVVVVIGGLGSLRGAIVAGLMVGVAVAMTIQFWPVASTSIMYVLMIVVL
                     LMRPRGLFGEQWERFE"
     gene            complement(1384..>2541)
                     /locus_tag="VIR04_05685"
     CDS             complement(1384..>2541)
                     /locus_tag="VIR04_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460272.1"
                     /translation="EPFIRTARAAEPIKLGVLLPKSGTYAVQGEQGHNGATIAVDDVG
                     GKVLDRPVQLVWLDENGPQTTQQNMHKLVQEQKVVAVQGGVSSGDVLAIMPVAEREKV
                     LLMATGPNATEITGANCNRYTFRVDLPNYETVRSLYPLLSKNGKSWYFLYASYAWGID
                     GYKQMNELLVKDGGKVVGADQTPLGTTDFSSYILKLMSAKPDVIFLGLGGGDMTNFLK
                     QFHQMGLTSKIPVSSLIVNDTDLWAAGPDAATGIYPKIWNYTGSQNTSISEQFAKKYQ
                     AKFGKPPESEAWQDWFGCTAILTAIRETGSTQTDKLVDFLEKHQFDGYKKQAIYFRPW
                     DHQLIQPTLLAKVKSKIESQYDYFDIVGTAPSGNETLEMLYGSKKTTECKI"
BASE COUNT          588 a          736 c          722 g          495 t
ORIGIN      
        1 ccccgccggt cagactggtc cagcgaaaac aaatctcgta gaacagttgc gtgaacgcca
       61 gggtgagcaa tgcgaaatac aggccgcgcc gtctaaggat cagcaagccc agcacgagac
      121 ccagcgccgc tccgaacgcg gtgctcacga tcagtccgag agcgacgttc tgaaccacat
      181 ggatggcaaa tagcgcgctg gcgtaactgc ccatgccgaa aaaaacagac gagccgaacg
      241 acaccatgcc tgtgtagccc agcaataagt tgtaggcgat gccatagagc gtatagataa
      301 ccaccagcgt ggcaaggctg accgtggatc ccgccgccga catcaagtaa ggcatgacga
      361 tgaaaacagc ggcaagcacg accagcggat gagtggcaag ttgacgcagg cgattcactc
      421 gaaacgctcc cattgctcgc cgaacaagcc gcgcggccgc atcagcagca ctacgatcat
      481 gagcacatac ataatcgagg ttgaagccac cggccagaac tggatggtca ttgccaccgc
      541 aacgccgacc atcaggccgg cgacgattgc gccgcgcaaa cttcccaggc ccccgatcac
      601 gaccacgacg aacgccggca tgatggcttg ctcggccatt gacggcacaa cactccacag
      661 cggcgcggca agcacaccgg cagcgcccgc aagcaggcag cccaggccga agatgaacat
      721 gaacacacgt ttcagattga tgcccagcat acttaccatc tctggatcgc gcgagccggc
      781 gcgaacgatc cggccatacg gggttttttc caaaaatagc cagacggcgg caagcaaggc
      841 gacggtgatc acaagcactg ccacgcggta gcgcgtaacg aacacgggcc cgtaatgcac
      901 tatcccggcc agaaaggcgg gcgtatcaaa tgggtggccc attgcgcccc atagcgtgcg
      961 gataagctcc tcgatgaaga gcgccagacc gaaagtcagc aacaggctga gcagcggttc
     1021 tttgttgtag agcgggcgca gcaatatgcg ctccaccaca aggccgatgg cgccgaccag
     1081 gataggggcc aaaaacagcg cgccggcaaa gccgacatgc gggcgcaacg acaatgcaat
     1141 gtaagcgccc agggcaaaaa atgcgccgtg ggcgaaattc accaggccca gcatgccgaa
     1201 aataatcgac atgccaacgg aaataaggac gaacaggaag cccagcacca gaccgttcat
     1261 aagctgcgac gcgaaaagcg tggcatttat cactgggcat ctccttttta cgcgaagcgc
     1321 ggcgcaagcg tgctggttgc ttgcgccgcg cggatttgaa cgccgcttat ggcgaggctg
     1381 tcgttatatt ttgcattcag tcgttttttt cgatccgtac agcatctcca gcgtttcatt
     1441 gccgcttggc gccgtgccaa cgatatcgaa ataatcgtac tgactctcga ttttggattt
     1501 gaccttggcc agcagagtgg gttgtatcaa ctggtggtcc cacggacgga agtaaatggc
     1561 ctgcttcttg tagccgtcga actgatgttt ttccagaaaa tccaccagct tgtccgtctg
     1621 ggtggatccc gtttccctga tggcagtaag gatggctgtg caaccgaacc agtcttgcca
     1681 cgcctcggat tcgggcggtt tgccgaactt tgcctgatat tttttggcga actgttcgct
     1741 gatgctggtg ttctgactgc ctgtgtagtt ccagatcttg ggatagattc cggtggccgc
     1801 gtcgggcccg gccgcccata ggtccgtgtc gttgacgatc agggatgaca ccggaatctt
     1861 gctcgtcagg cccatttgat gaaactgctt gaggaaattg gtcatgtcgc cgccacccag
     1921 gccaaggaaa atgacgtccg gcttggccga catgagcttg agaatataag aactgaaatc
     1981 cgttgtgccc agcggcgttt gatctgcgcc gacgaccttg cctccgtcct tgacaagcaa
     2041 ttcgttcatc tgcttgtagc cgtcaatgcc ccaggcatag gaagcgtaca gaaaatacca
     2101 gctcttgccg tttttcgaca gcagaggata aaggctgcgc acggtttcgt aattgggcag
     2161 gtccacgcgg aaggtgtatc gattgcaatt ggcgcccgta atctctgtag cgttggggcc
     2221 ggttgccatc agtaaaacct tttcgcgctc ggccaccggc ataatggcca gaacatcgcc
     2281 gctggagacg cctccctgaa cggcaacgac tttctgttcc tgaaccagct tgtgcatgtt
     2341 ttgctgggtt gtctgcggtc cgttttcatc cagccatacg agttgcacgg ggcggtcaag
     2401 caccttgccg cccacatcgt ccacggcgat agtcgcgccg ttatggcctt gttcgccctg
     2461 caccgcatag gtgccggatt tgggcagcag gacgcccagc ttgatgggtt ctgctgcgcg
     2521 cgctgtccgg atgaacggct c
//
LOCUS       DATCXU010000253         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_378022,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000253 DATCXU010000000
VERSION     DATCXU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378022"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIR04_05690"
     CDS             complement(<1..283)
                     /locus_tag="VIR04_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HEY9460273.1"
                     /translation="MRLISRMFNLNDPGWGRGNNDGSEPPRRPEKPNGPPDLDEVWRD
                     FNNRLGALFGRKPKRGGQFGGGGGGHNGANFQLPKSSPKMLIIGVIVIVA"
     gene            complement(258..1355)
                     /gene="hflX"
                     /locus_tag="VIR04_05695"
     CDS             complement(258..1355)
                     /gene="hflX"
                     /locus_tag="VIR04_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY9460274.1"
                     /translation="MKALIVSVDLGSPDYQAHAEEFVMLARGAGADIVGTLVARRKRP
                     DAAYFIGSGKLQEGVLLAQQTQAEIILFDQALTPAQQRNLERGFDLRVVDRVALILDI
                     FALRAKSHEGKLQVELAQLQHLSTRLTRLWTHLERQRGGIGFRGPGEAQLEMDRRLIG
                     GKVRVLRDRLERVQRQRATQRRSRSRNSIFSVSLVGYTNTGKSTLFNALTRADAFAAN
                     QLFATLDTTTRKVWIEGTGQVVISDTVGFIRDLPPTLIAAFRATLEETVQADLLLHVV
                     DASSQQRDEQIAQVDKVLADIGAAEIPRILVYNKVDAAGYTSRVERDEHGTIARVFVS
                     ALKRTGLDGVRQAIVESGQIAGNNAFNIENV"
     gene            complement(1430..1666)
                     /gene="hfq"
                     /locus_tag="VIR04_05700"
     CDS             complement(1430..1666)
                     /gene="hfq"
                     /locus_tag="VIR04_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY9460275.1"
                     /translation="MSNKGQTLQDPFLNALRKDHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTVTQMVYKHAISTVVPSRPVNIQVDEPAE"
     gene            complement(1778..>2743)
                     /locus_tag="VIR04_05705"
     CDS             complement(1778..>2743)
                     /locus_tag="VIR04_05705"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY9460276.1"
                     /translation="ERVQAIKEAADDRLRLYPDYDSSVLRQAIATLYGLQANQVFLGN
                     GSDEILAHVFNGLFRHSGKRLMMPDISYSFYRTYCRMFQVDPEIIPLADDFSIRASDY
                     TAPSRQPCAGVIFANPNAPTGMALSLTEIEQIVGGNPDCAVVVDEAYVDFGAQSAVGL
                     LARYDNLVVVQTFSKSRSLAGLRVGFAMASASIIDGLVRIKDSFNSYPLDRLAQAGAL
                     AALQDTAYFEQARLKVMQTRDQLLIPDLCALGFQVLPSHANFVFARHPDYNALDIGKA
                     LRERHILVRYFAQPRIDQFLRITVGTPGECKRLCNALAEIVGRPD"
BASE COUNT          668 a          779 c          705 g          591 t
ORIGIN      
        1 ccgccacgat aacgatgaca ccgataataa gcattttcgg cgagcttttc ggcaattgga
       61 aattggcgcc gttatgaccg ccgccgcccc cgccaaattg accgccgcgc ttgggcttac
      121 ggccgaaaag cgcaccaaga cgattgttga agtcgcgcca gacctcgtcg aggtcgggtg
      181 gcccattagg cttttcggga cggcgcggtg gttctgagcc gtcgttattg ccgcgacccc
      241 aacccgggtc attcaaatta aacattctcg atattaaacg cattatttcc cgcgatttga
      301 cctgactcga caattgcctg acgcacaccg tccagcccgg tgcgctttaa agcgcttaca
      361 aagactcgcg caatggtacc atgttcatcg cgttcaactc gcgatgtgta gcccgccgca
      421 tcgaccttgt tatacaccag aattctgggg atctccgcag caccgatatc ggccaatacc
      481 ttgtcaactt gagcgatttg ctcgtcgcgc tgctggctgg acgcgtccac cacatgaagc
      541 aacaaatcgg cctgaaccgt ttcttcaagt gtagcccgga acgccgcaat caaggtagga
      601 ggcaggtctc gaataaaccc gacggtgtct gaaataacca cctgccccgt cccttctatc
      661 cataccttgc gagtcgtcgt atccagcgtc gcgaataatt gattcgccgc gaaagcatcc
      721 gcacgggtca gcgcgttgaa caaggtggat ttgcccgtgt tggtgtaccc gaccagggat
      781 actgaaaata tactgttgcg cgagcgcgaa cggcgttgcg tggcgcgttg gcgttgcacg
      841 cgctctagcc ggtcgcgcaa aacccgaacc ttgccgccaa tcaggcgccg atccatttca
      901 agttgcgctt cgcccggccc gcgaaacccg atgcctccgc gctgacgctc aagatgggtc
      961 cacaggcgcg tcaagcgagt ggacaaatgc tgcaactggg cgagctccac ttgcaatttg
     1021 ccttcgtgac tttttgcccg cagagcgaaa atatcaagaa ttagcgcgac gcgatccacc
     1081 acccgcaaat caaatccgcg ctcaagattg cgttgctgtg ccggtgtaag cgcctgatca
     1141 aaaaggataa tttcagcctg ggtctgctga gccagcaata cgccttcttg caacttgccc
     1201 gatccaatga aataagcggc gtcgggacgc ttgcgacgcg ccacgagcgt gccaactata
     1261 tcggcgccgg ccccgcgcgc gagcatgacg aattcttcag catgcgcctg gtaatcggga
     1321 ctgcccaggt cgacgctgac aattaatgct ttcatgccca tttcataaac gaaatacccg
     1381 ccggcgccag gcacacagcg ggtgtttcac cgaaaataag cgggttggac tattcagcgg
     1441 gttcatccac ttgtatgtta accgggcgag atgggacaac ggtcgaaatg gcgtgcttgt
     1501 aaaccatttg agttacggta ttacgcagca aaacaacata ctgatcaaac gattcaacct
     1561 gcccctgcag cttgatacca ttgacaagat aaatcgacac gggcacgtgg tccttgcgta
     1621 atgcatttaa aaacggatct tgtagtgttt gccctttatt gctcattggc taggctccaa
     1681 ttatgttgtt attaaactaa tagaaagaca gttactttac agggttttcc cgtatcgtgc
     1741 catacagcag cgtattttac gctctgacat caggttttta atcgggcctt ccgactattt
     1801 cggccaatgc attacataga cgcttgcact cgcccggagt tcccaccgta atacgtaaaa
     1861 actggtcaat tctgggctga gcgaagtaac gcaccagaat gtggcgctcg cgcagagctt
     1921 tgccaatatc cagcgcgttg taatcgggat ggcgggcaaa tacaaaattg gcgtgcgatg
     1981 gcagcacctg gaaacccaaa gcgcacaaat ccggaatcaa taattgatcg cgcgtctgca
     2041 tgactttcag gcgcgcttgt tcgaaatagg cggtgtcctg cagtgccgcc agggctccag
     2101 cctgcgccag acgatcgagc ggatacgaat taaagctgtc tttaatgcgc accaggccgt
     2161 cgataatact ggcactggcc attgcgaaac caacccgcag acctgccagc gaacgcgatt
     2221 tggaaaacgt ttgcaccaca acaagattat cgtaacgcgc cagcaacccg acagccgatt
     2281 gggcgccaaa atccacgtac gcttcgtcaa ccacgaccgc gcaatcggga ttaccgccaa
     2341 cgatctgctc gatttcagtc aacgaaagcg ccatgccagt cggcgcgttc ggattggcga
     2401 aaattacacc ggcgcaaggc tgacggctgg gggcggtata gtccgaggcc cgaatcgaaa
     2461 aatcgtcggc caaagggatg atttcgggat ccacctgaaa catgcggcaa tatgtacggt
     2521 aaaaactgta cgatatgtcg ggcatcatca gccgttttcc ggaatgcctg aacaggccat
     2581 tgaagacatg agccagaatt tcatccgaac cattgccaag aaatacctga ttcgcctgca
     2641 gtccgtacaa cgtcgcgatg gcttgacgca aaacactgga atcgtagtcg ggataaaggc
     2701 gcaggcggtc atccgcggct tccttgatag cctgcacacg ctc
//
LOCUS       DATCXU010000254         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_871492,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000254 DATCXU010000000
VERSION     DATCXU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871492"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VIR04_05710"
     CDS             <1..1424
                     /locus_tag="VIR04_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY9460277.1"
                     /translation="TGASPVASIKHAGTPWELGLAETQQTLLINNLRSRIRVQADGQM
                     KTGRDVAIGAMLGADEFGFATAPLVVEGCIMMRKCHLNTCPTGVATQDPVLRKKFSGK
                     PEHVVNYFFFVADEVREIMAQLGIRTFDELIGRVDLLDLRPGIEHWKARGLDFTRVFH
                     QVVTTSDKHQTKEQDHGLAAALDHQLIERSKGALENGEKVSFIVPVRNRNRTIGAMLS
                     GQVAVRYGHEGLPDDTIHIQCNGAAGQSFGAFLAHGVTLDLVGEANDYVGKSLSGGRI
                     IIRSPNDFRGFGPDHIIVGNTVLYGALEGEAYFNGVAGERFAVRNSGAIAVVEGTGDH
                     GCEYMTGGIVVVLGETGRNFAAGMSGGVAFVWDPDHTFRQRCNMNMVELESIVSNEEQ
                     VKANNMETWHSVKRGGKRETDETILRRLIEDHYRYTGSFRSRDILGDWEDSRKAFVKV
                     IPTDYRRAISELWRTAHPKQIAA"
     gene            1435..>2439
                     /locus_tag="VIR04_05715"
     CDS             1435..>2439
                     /locus_tag="VIR04_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY9460278.1"
                     /translation="MGKITGFMEFDRVKEVTEAPEKRVKHWNEFTSTLTDEQAKIQAA
                     RCMDCGIPYCTTGCPVNNIIPDWNDLVYRQDWRNALDSLLSTNNFPEFTGRICPAPCE
                     TACSLAINDEAVGIKSLEHAIIDKGWAEGWVVPQPPSRKTGKKVAIVGSGPSGLACAQ
                     QLARVGHAVTVFEKNNRIGGLLRYGIPNFKLEKSHIDRRLAQLEAEGVEFCPSTYVGD
                     PANAADGLTVLTPEFLQSEFDAIVLAGGAETPRDLPVAGRELSGVHFAMEFLPQQNKV
                     LAGDRINKQIVATGKRVIVIGGGDTGSDCVGTSNRQGAISVTQFELMTKPPEDPSVYM
                     T"
BASE COUNT          548 a          713 c          730 g          448 t
ORIGIN      
        1 gcaccggcgc atcgcccgtg gcatccatca agcacgccgg caccccatgg gagcttgggc
       61 tggcggaaac ccagcaaaca ctgcttatca ataacctgcg cagccgcatc cgtgtgcagg
      121 ccgacggcca gatgaagacg ggccgcgacg ttgccatcgg cgcgatgctg ggcgccgacg
      181 aattcggttt tgccacggcg ccgctggtag tcgagggctg catcatgatg cgcaaatgcc
      241 atctgaacac atgccccaca ggggtcgcca cgcaagatcc ggtgctgcgc aagaagttct
      301 ccggaaaacc cgagcatgtg gtcaactatt tcttctttgt ggccgatgaa gtgcgcgaaa
      361 ttatggcgca attgggcatt cgcacttttg acgaattgat tggacgcgtc gatcttcttg
      421 acctgcggcc cggtattgaa cactggaagg cgcgcgggtt ggactttact cgcgtgttcc
      481 accaggtcgt caccacgtcg gataagcacc agaccaagga acaagaccac ggcctggcgg
      541 cggcactgga tcatcagttg atcgaacgca gcaaaggcgc gctggaaaac ggcgaaaagg
      601 tgtcgtttat cgttcctgtg cgcaaccgca accgcaccat cggcgccatg ctgtcaggcc
      661 aggttgctgt gcgctatggc cacgagggtc tgcccgacga caccatacat atccaatgca
      721 acggcgccgc cggacaaagc ttcggcgcct tcctggcgca cggcgtgacg cttgacctgg
      781 tgggcgaagc caacgattac gtgggcaaaa gcctgtcggg agggcgcatt attatccgct
      841 cgccaaacga cttccgcggt ttcgggcccg accacatcat cgtgggcaac acggtcttgt
      901 acggcgccct ggaaggcgaa gcgtatttca acggcgttgc gggtgagcgt ttcgctgtgc
      961 gcaattccgg cgcgatcgcc gtcgtcgaag gcacgggcga ccatggctgc gaatacatga
     1021 cgggcggcat cgtggttgta ctgggcgaaa ccggccgcaa ttttgcggcg ggcatgtccg
     1081 gcggcgtggc cttcgtgtgg gatcctgacc atactttccg ccagcgctgc aacatgaaca
     1141 tggtcgagct tgaatccatc gtatccaacg aagaacaggt caaggccaac aacatggaaa
     1201 cctggcacag tgtcaaacgc ggcggcaagc gggaaaccga tgaaaccatt ttgcgccgtc
     1261 tgattgaaga ccactatcgt tacaccggta gtttccgctc gcgcgacata ctgggcgact
     1321 gggaagattc gcgcaaagcg ttcgtcaagg tcatacccac cgactaccgc cgtgcaataa
     1381 gcgaattgtg gcgcacggca cacccgaaac aaatcgccgc ctgaggatat cgagatgggc
     1441 aaaataactg ggttcatgga attcgaccgc gtcaaggaag tgacggaagc gcccgaaaag
     1501 cgcgtcaagc actggaatga attcacctcc acactgaccg acgaacaggc caagatacaa
     1561 gcggcgcgct gcatggactg cggcataccc tactgcacca caggctgccc ggtaaacaat
     1621 atcattcccg actggaacga cctggtttac cgccaggatt ggcgcaacgc gctggactcg
     1681 ctgctatcga ccaacaattt ccccgagttc acagggcgaa tttgccccgc tccatgcgaa
     1741 acagcgtgtt cgcttgccat caacgatgag gccgtcggca tcaagtcgct tgaacacgcc
     1801 atcatcgaca aaggctgggc ggaaggctgg gtggtgccgc aaccgcccag ccggaagaca
     1861 gggaaaaaag tcgccattgt cggttctggg ccatcgggcc tggcctgcgc gcaacaattg
     1921 gcgcgcgtcg ggcatgccgt gaccgtgttc gaaaagaata atcgcatagg cggcttgttg
     1981 cgttacggca taccgaactt caagctcgaa aaatcccata tcgaccggcg cctggcgcaa
     2041 ctggaagccg agggggtcga gttctgtccg tcgacgtatg tgggcgaccc tgcaaacgcc
     2101 gcggatggtt tgacggtgct tacgccggaa tttcttcagt cggaatttga tgccattgtg
     2161 ctcgccggag gggccgaaac tccgcgcgat ttgccagttg ccggccgcga actgagcggc
     2221 gtgcatttcg ccatggaatt cctgccgcaa caaaacaagg tgctggcggg cgatcgcatc
     2281 aacaagcaaa tcgtggccac cggcaagcgc gttattgtga tcggaggcgg agatacgggt
     2341 tcggattgcg tgggcaccag caaccggcaa ggcgcgatat cggtcactca attcgagctc
     2401 atgaccaagc cgcccgaaga ccccagcgta tatatgacc
//
LOCUS       DATCXU010000255         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_532412,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000255 DATCXU010000000
VERSION     DATCXU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532412"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VIR04_05720"
     CDS             <1..1141
                     /locus_tag="VIR04_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460279.1"
                     /translation="LSDEKEHEYFADGMVEDLVTGLSRIKSVFVIARDSSFIYKGRAV
                     DLKQVGRELCVRYVLEGSIRKADNRIRVAVQLSEAQSSARLWAESYDRPFQDIFTVQD
                     DIAMKVIGAIEPQIRKIEIERINRKRPNNLDAYDLLLRAQSLRYEMPEAAQKAIALLE
                     QALALEPNYTSAHAFLALCYHAQFSHGGWDEEDRKAAIQHAHAVVTIGSDDGTTLALA
                     GFVLAFDEHNVVTSLELFDRALEISSSNIVALSLSAVVHAWIGNAELAIERAQRALAV
                     SPLGNSASYGALSIAYFSKKRYAEARDAALRANAMRPQYIVNYILLTVSLFRLGYLEE
                     AKAMGTRVIELDPNFTMKRWSITAGIVPDMFKPFADAWREMGMPV"
     gene            complement(1736..2608)
                     /locus_tag="VIR04_05725"
     CDS             complement(1736..2608)
                     /locus_tag="VIR04_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY9460280.1"
                     /translation="MQLVNWPLGKADDIRNKMSDSALETVLRRDRALVAAALGVIVVL
                     AWGYVLWLAADMDMGGMDMTGLRMIPAGMGLMAPANAPWGAIEFAFVFVMWAVMMVGM
                     MAPSVAPMILMYARVGRQGRAEGKPLAATGWFAAGYFLTWIGFSLAATLAQWMVERAA
                     LLDSRMASASNMLGGVVLIAAGVYQWTPLKDICLAQCQSPLQFLMRHGGFRGDWSGCL
                     LLGFRHGAYCVGCCWVLMALLFVGGVMNVLWIALLALLVLLEKLTPFGRWVARSVGAA
                     CVAAGAWMLLVSPQ"
BASE COUNT          653 a          772 c          738 g          539 t
ORIGIN      
        1 tttgagcgac gaaaaggagc acgaatactt cgcggacggc atggtggaag accttgttac
       61 gggactttca aggatcaagt cagtcttcgt catcgcccgg gattcaagtt tcatctacaa
      121 aggaagagca gtcgacctga agcaggttgg ccgtgaactc tgcgtccgat acgttcttga
      181 agggagcata cgaaaggcgg acaaccgtat ccgtgtcgca gtacagctta gcgaggcgca
      241 atccagcgct cgtctttggg ccgagagcta cgatcgacct ttccaggaca ttttcaccgt
      301 gcaagatgac attgccatga aggtgatcgg ggcaatagaa ccgcaaatta gaaaaattga
      361 aattgagcgc atcaaccgca agcgaccgaa taatctcgat gcttatgatc tcttgctacg
      421 tgctcaatcg cttcgatatg aaatgccaga agctgcgcaa aaggccattg cattgcttga
      481 acaggcattg gcgctcgaac cgaactacac cagcgcgcac gcgtttcttg ccctttgtta
      541 tcatgcccaa ttcagtcacg gtgggtggga cgaggaggat cgcaaggctg ctattcaaca
      601 tgcgcatgcg gtggtaacaa tcggtagcga tgatgggacg acgctggcat tagcgggatt
      661 tgtgctcgca ttcgatgagc ataatgtcgt cacatcgtta gaactctttg atcgtgccct
      721 tgagatcagc agttcgaata tagttgcact tagtttaagt gcagtcgttc acgcttggat
      781 agggaacgct gaacttgcta tcgaaagggc gcaacgcgcg cttgccgtca gccctctcgg
      841 taattcagct tcttacggag cgctttcgat cgcgtatttt agcaaaaaac ggtatgcgga
      901 agcgcgggat gcagcgctgc gagccaacgc aatgagaccg caatacattg ttaattatat
      961 tctacttact gtatcgctgt ttcgtcttgg ttacctcgag gaagcgaagg cgatgggcac
     1021 tcgagtgatt gaacttgacc cgaattttac gatgaagcga tggtccatta cggccggaat
     1081 tgtgccggat atgtttaagc cattcgccga tgcgtggcgt gaaatgggaa tgccagttta
     1141 acttattgcg tcggccggtg ccgtcatcaa gcgattataa aaagcagcac gatgagtttc
     1201 aggcgtaagc taaatcgcaa gctaccctaa atccaaaacg aagccgccat ttttaaactg
     1261 cggaagtcca tttcgggtca gaagtggatc tcaaagcggt caaacgctac gtccctcttg
     1321 ccctttaaag cggacaagtc cgaattttta ctctcacgac agcgcgggaa tttcgggttt
     1381 cttgcgccag attggggcca gagactttcc acgcgacaag gttgccggaa cgcatgggga
     1441 caccctactc cctaagcgaa cacagcgacg tatcggagcg cggaagcgcc tcggcagggc
     1501 ggcccaatcc aaccccctgg cgccaagacc cactgggtaa tgcgcagagc tcgatcaaac
     1561 tcagtctctg ccgacgggtc tacgaatttg ggaatatttg taaggcgcca gccccagaga
     1621 ccggttcgta caacgtgtcc tcctttgccg tcgaaagcgg gcatccgcca gtgcgactgg
     1681 cgtgtaaacg cgcgagccac ttacctgagg gctcccaggc tttgccgcgc agacatcact
     1741 gtggtgaaac taacaacatc caggcgcccg cggcgacaca agcagcgccg acactgcgcg
     1801 cgacccaccg gccgaacggg gtcagtttct ccagaaggac aagcagcgcc aggagtgcga
     1861 tccaaagcac gttcatgacc ccgcccacaa acaaaagcgc catcagaacc cagcagcagc
     1921 cgacgcagta agcaccgtgc cgaaacccaa gcagtaggca gcccgaccaa tcgccgcgaa
     1981 agccgccgtg acgcatcagg aactggagcg gtgactggca ttgggcgaga cagatgtcct
     2041 tgagcggggt ccattggtag actcccgccg caatcaacac gacacctccg agcatgttgc
     2101 tggcgcttgc catccgagaa tcaagcaagg ccgcccgttc aaccatccat tgggccaggg
     2161 tcgctgccag tgaaaaaccg atccaagtca ggaaatagcc ggcggcgaac cagcccgtcg
     2221 cggcaagcgg cttgccctca gccctcccct gtcgacccac gcgggcgtac atgaggatca
     2281 taggtgcgac cgagggcgcc atcatgccaa ccatcatcac ggcccacatg acgaacacaa
     2341 acgcgaactc gatcgctccc catggcgcgt tcgccggcgc catgagcccc atgccggccg
     2401 ggatcatgcg caacccggtc atgtccatgc cgcccatgtc catgtcggca gcaagccaga
     2461 gcacgtaacc ccatgccagc acgacgatca cgccgagcgc agcagcgacg agtgcgcgat
     2521 ctcgtcgcag caccgtttcc aatgcgctgt cgctcatttt gttacgaatg tcgtcggcct
     2581 taccgagggg ccaattcacg agttgcatca cgactgattt gaccagcgga tcggcgcgta
     2641 gtggccgttc ttgccggaat tatcccaacg catgccataa tcgctgaagg tattgcccgc
     2701 cg
//
LOCUS       DATCXU010000256         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_160137,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000256 DATCXU010000000
VERSION     DATCXU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160137"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..877
                     /locus_tag="VIR04_05730"
     CDS             185..877
                     /locus_tag="VIR04_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460281.1"
                     /translation="MSFTKSLLALALVAASTLVYAGGTAQVQGGDKNITVEFDGSFAR
                     VQSDQQKNVHLLARDNAVYAVTDMGGQPLVVESSAVIGLLGAQGMAKLENDKDAIQKF
                     VSLDATGRNETVAGFQGSVYKLVYTDGNGKRRSEEAVLGKQPSIVELSQSLGHVAINL
                     MQLAKHDTRGSTELVNELRKRNLGLLRFGAKLRLISLNTAAPNASRFVLPSAPMDLSQ
                     GLGGLLQGMGRK"
     gene            complement(893..1888)
                     /locus_tag="VIR04_05735"
     CDS             complement(893..1888)
                     /locus_tag="VIR04_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY9460282.1"
                     /translation="MGKVVIISTGGTITMTGGSAETGIEPTLTAGDLVAAVPELARAA
                     EILAITAYRKPSASLTIENIVKLAGDIDGYLAGDVDGAVVIQGTDTIEETAFLLNILI
                     ESDKPVVVTGAMRGAQMPGADGPANLLASVHVANAGTSAKRGTLVVLNDEIHSATFVQ
                     KSHTALPSAFMSPTTGRVGLVSEGHVLFTSPPARSTRYHIKPSTIKPVALLKVSIGDD
                     GRLLSGLVASGFTGLVLEGMGAGHVPETWVEPLQALARQIPVVLAVRVPSGPVFSSTY
                     GFPGSERDLLARGLISAGLLGALKARLLLTLLLSGGASRARVLDAFSDFTPWSDE"
     gene            complement(1953..2861)
                     /locus_tag="VIR04_05740"
     CDS             complement(1953..2861)
                     /locus_tag="VIR04_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY9460283.1"
                     /translation="MTSPLSPRDRLPYSAIIDRPALPAPNGARLIVWPVVNIEHWDTE
                     RPMARRVLPPPTGINAIPDIPNWSWHEYGMRVGFWRLKATFDRLGITPTLSINGKVCV
                     SYPRVTQAAVDAGWEFMGHGFTQMPLHQVDDQPAMIAQTIEHIKRATGRTPAGWLGPG
                     LTETLDTVDLLHAAGIRYVGDWVLDDEPCRLITPTGPMVAMPYSVELNDVSIMAVQTH
                     SAPEFFTRVRDSFDRLYQEGKERVHVMAVAVHPFLSGVPHRVKYFEQAFDYIAQHKDV
                     CFMTGEQILDWYNKVSPVDTDVSKKV"
     gene            complement(2952..3656)
                     /locus_tag="VIR04_05745"
     CDS             complement(2952..3656)
                     /locus_tag="VIR04_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460284.1"
                     /translation="MLDVKELQSFYGDAHVLQGASLTVQQGEIVCLLGRNGMGKSTLI
                     RSIMGLTPPDIRGGAIVWKGENIRGLRPHKIAQRGIALVPQGRRLFPTLTVTEHLTVL
                     KRMSATHGWTVSKAFEFFPRLAERRHHRGGQLSGGERQMLAIARALMTDPELILMDEP
                     SEGLAPVMVQHLEDIIQMLKQENLSILLVEQNLYSALAVADRLYILESGRVVYEEECA
                     AVRQNPEALTQFLGVH"
     gene            complement(3649..4413)
                     /locus_tag="VIR04_05750"
     CDS             complement(3649..4413)
                     /locus_tag="VIR04_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460285.1"
                     /translation="MSPVNDLLILDRVTIRFGGLCAVDDVSIRIGAQERRAIIGPNGA
                     GKTTLFNAITGVIRPTSGKIIFQDQDITNTPIHARTKQGISRTFQITNLFPNLTVQEN
                     MQLAAYGLSPRKFSILGKIVLDANARAHADAALTFSRLQERVHTPVKELSYGEQRQLE
                     LAMALTAQPKLLLLDEPAAGLSPPERVILADIIAALPRSLPIVLIDHDMDLVLQLVDY
                     VTVLNNGKLLAQAPPQQIRENVDVQNVYFGKARQHA"
     gene            complement(4418..>5076)
                     /locus_tag="VIR04_05755"
     CDS             complement(4418..>5076)
                     /locus_tag="VIR04_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460286.1"
                     /translation="AQLWHPINTSDFSPYIGQLASLGVDVVFAMETGSDATRLIQQYN
                     NFGLKGKIPLVSAMNVTDQSVIRTMGAEAEGIVSAAQFAEGADNPKTQEFVREYSRRF
                     NKLPSLYAFTMYSGAMWVKEALETVKGDAEDRKAFLKAVMDTRLTGSPLGKEVTLDAY
                     GNPIYDVYIRKVEKRPDGKYWNVPIATYPGVSQFWHYDPEKYMQQKPYSRNFQGYSKS
                     "
BASE COUNT         1080 a         1354 c         1491 g         1151 t
ORIGIN      
        1 agcccacgag cgcggtgatc atagccaagg actggggttt cacgtgaggg ttctcctgat
       61 agtcgttgat cttagtcagg catggcgccc agtgtcaaca aatgggctgt ttgctcaatg
      121 gtagaattta atgcggtggt ctgtgggcgg cttgcgtttg cggaaatctt ctggggtgcc
      181 cttcatgtca tttacaaaat ctttgctcgc gcttgctctt gtggcggctt ccacgctggt
      241 ttatgcgggc ggcacagcgc aggtgcaagg cggcgataaa aatataacgg tggaatttga
      301 cggctcgttt gcgcgcgtgc agtccgatca gcaaaagaat gttcacctgc tcgctcgcga
      361 taatgcggta tacgcggtga cggacatggg cgggcaacca ctggtcgtcg aaagcagcgc
      421 ggtgattggg ctattgggcg cccagggaat ggcgaagctc gagaatgaca aggacgccat
      481 tcaaaaattt gtttcgctcg atgcgacggg ccgaaatgag acggtcgccg gatttcaggg
      541 ttctgtatat aaactggtct ataccgacgg caacggcaaa cgccgatccg aagaagctgt
      601 tttgggcaag cagccatcca tcgtcgaatt aagtcaaagc ctgggtcacg tggcgataaa
      661 cctgatgcag cttgccaaac acgatacgcg cggttcgacc gaattggtaa atgagctgcg
      721 gaagcgtaat ctgggcctat tgcggtttgg tgcgaaattg cgattgatca gtctgaatac
      781 cgccgcacca aatgcttcac gatttgtttt gccgtcggcg cccatggact tgtcgcaagg
      841 cttgggcggc ttgttgcagg ggatggggcg aaagtaagcg cccgactgcg aatcactcat
      901 cggaccatgg cgtaaagtcg gaaaaggcgt ccagcacacg tgcgcgtgaa gcgccaccgc
      961 ttaacaataa ggtcagcaat aatctggctt tcaaggcgcc aagaaggccg gccgatatca
     1021 ggccgcgggc gagcagatcg cgttccgaac ccgggaagcc gtaggtgctg gaaaatacgg
     1081 gcccgctggg gacacgcacc gcaagcacca cgggtatttg tcgggcaagc gcctgtagcg
     1141 gttccaccca ggtttcggga acgtgcccgg cgcccatgcc ttcaaggacc agtccggtga
     1201 atcccgacgc gaccaggccg gacaaaagcc ggccatcatc gcctatcgag actttcaaaa
     1261 gcgcaacagg ctttatggtt gatggtttaa tgtggtatcg ggtggaacgc gctggcggcg
     1321 aagtgaaaag gacgtgtcct tcagacacaa ggccgacgcg ccctgtcgtt ggcgacatga
     1381 aggcggaagg cagagcagta tggctctttt gcacaaacgt tgcactgtgg atttcatcat
     1441 tcaacaccac cagcgtgcct cttttggccg aagtgcccgc gtttgccaca tggacggatg
     1501 ccagcaggtt tgccgggcca tccgcacccg gcatttgtgc gccgcgcatg gcgccggtca
     1561 ccaccacagg cttgtcgctt tcgatcagaa tattaagcaa aaacgctgtt tcttcgatgg
     1621 tgtcggtgcc ttgtatgact accgcgccgt caacgtcccc cgccagatat ccgtctatgt
     1681 cgccggcaag cttgacaatg ttctcaatgg tcaatgacgc gcttggcttg cgatacgcgg
     1741 taatggccaa aatttcagcg gccctggcaa gttcgggcac tgcggcgacc agatcgccgg
     1801 ccgtcagcgt tggctcgatg cctgtttcag cagagccgcc ggtcatcgta atggtgccac
     1861 cggtggaaat aatgacgact ttacccatga ggtgaaactc cttattgtga ccgatgcggt
     1921 tgtgccggcg ggtggatgcg attagtgtga tcttaaactt tcttgctgac gtcggtgtcg
     1981 acgggagaga ccttgttgta ccagtccagg atttgctcgc ccgtcatgaa acacacatcc
     2041 ttgtgttgcg cgatatagtc gaatgcctgt tcgaaatatt taacccgatg gggcacgccg
     2101 ctaaggaacg gatgcaccgc aacggccatg acatgcacac gttctttccc ttcctgatac
     2161 agccgatcaa aactatcgcg cacgcgcgtg aaaaattcag gggcgctgtg ggtctggacc
     2221 gccattatgg aaacatcgtt gagttcgact gaatagggca tggccaccat cggtccggtc
     2281 ggcgtaatca gacgacaagg ttcatcgtcc agaacccagt cgccgacgta tcggatgccg
     2341 gctgcatgca gcaagtcgac cgtgtccagg gtttctgtca ggccggggcc aagccagccc
     2401 gctggcgtcc gccccgttgc gcgtttgatg tgctctatcg tttgcgcgat catcgcgggc
     2461 tgatcgtcca cctgatgcaa cggcatttgc gtaaatccgt gccccatgaa ttcccatccg
     2521 gcgtcaacgg cagcctgagt aacccgcggg tagctgacgc acactttccc attaatggaa
     2581 agtgtgggcg tgatcccgag gcggtcgaat gtggctttga ggcgccagaa gcccacgcgc
     2641 attccgtatt cgtgccagga ccagttcggt atgtcgggga tggcgttgat gccggttggg
     2701 ggcggcaaca ctcggcgggc cattggacgt tccgtgtccc aatgttcgat attgacgacg
     2761 ggccagacga tcaggcgggc gccattgggc gcaggaagcg cagggcgatc aataatggcg
     2821 gaataaggca ggcgatctcg tggcgataaa ggggaagtca tgatattcat ttaataaatg
     2881 ttgggcggtt tagaggtgaa ttcctgcggt tcaatctacc cacctgggta ggtactgtca
     2941 atatctgcca tttaatgcac gcccaggaat tgagtcaggg cctccgggtt ctgtctgact
     3001 gccgcgcatt cttcttcgta gaccacgcgc ccggactcca gaatatacag acggtcggcg
     3061 acggccagcg cactgtacaa attctgttcc accagcaaaa tggacagatt ttcttgcttg
     3121 agcatctgaa tgatgtcttc cagatgctga accataacgg gcgcaagacc ttcggacggc
     3181 tcatccatca ggatgagttc aggatcagtc atcagcgcgc gcgcaatcgc cagcatctgc
     3241 cgttcaccgc ccgaaagctg accgccgcga tggtggcgcc gctcggccag gcgcggaaag
     3301 aactcgaatg cttttgacac ggtccagcca tgcgtggcgc tcatgcgctt taatacggta
     3361 aggtgctctg tgaccgtcaa tgtcgggaac agccgccgcc cctggggcac tagcgcaata
     3421 ccccgttgag caattttatg gggccgcagc ccacgtatgt tttcgccctt ccatacgatg
     3481 gctccgccgc gtatgtccgg tggcgtcagg cccatgatcg agcgaatgag tgttgatttc
     3541 cccatgccat tacgcccaag aaggcagacg atttcgccct gttgcactgt aaggcttgcg
     3601 ccctgcagca catgtgcgtc gccgtagaaa ctttgcaatt cttttacgtc aagcatgctg
     3661 acgggccttg ccgaagtaga cgttctggac gtccacattt tcccgtattt gctgcggagg
     3721 cgcctgcgca agaagtttgc cgttgttcag cacggttacg tagtccacca attgaaggac
     3781 caaatccata tcatggtcga taagcacaat gggcaggctg cgcggcaacg ccgcgataat
     3841 gtccgccagg attacgcgct ctggcgggga aaggcccgcc gcgggttcgt cgagcagcag
     3901 cagtttgggc tgggcagtca aagccattgc aagctccaac tgacgttgtt cgccatacga
     3961 caactctttc actggcgtgt gtacgcgttc ttgcaggcgc gaaaacgtca gagctgcatc
     4021 ggcgtgcgcg cgtgcgtttg cgtcaagcac gattttgccc aatatggaga atttccttgg
     4081 tgaaagaccg tacgcggcca gttgcatgtt ttcctgcacg gtcagatttg gaaagagatt
     4141 ggtgatttga aacgtgcggc ttatgccctg tttggttcgc gcatggatgg gcgtgttggt
     4201 gatgtcctga tcctgaaaga tgatttttcc gcttgtgggc ctgatgacgc cggtgatggc
     4261 attaaacagt gtcgttttgc ctgcgccgtt cggcccgatg atggcccggc gctcctgtgc
     4321 gccgatgcga atcgagacat cgtcgacggc gcacaggcca ccgaaccgaa tggttacgcg
     4381 atcaaggatc aggaggtcat tcacggggct caccacgtca gctcttgctg tagccctgga
     4441 aattgcgcga atacggtttt tgttgcatgt acttttcggg gtcgtaatgc cagaattgcg
     4501 aaacgcccgg ataggtggcg ataggaacat tccagtattt gccatccggg cgtttttcaa
     4561 ccttgcgaat gtacacgtcg taaattgggt tgccataggc atcgagcgtg acttccttgc
     4621 ccagaggcga ccccgtaagc cgcgtgtcca taacagcttt aaggaaggcc ttccggtctt
     4681 cggcgtcgcc ttttactgtt tccagggctt ctttcaccca catagcacct gaatacatcg
     4741 tgaatgcata aagcgatggc aatttattga atcgcctgga gtactcgcgg acgaattcct
     4801 gcgttttggg gttatcggcg ccttcagcga attgtgcggc ggaaacaata ccttcagctt
     4861 cagcccccat cgtacgaatc acggactgat ccgtaacgtt catggcggaa acgagcggaa
     4921 tttttccctt caggccaaaa ttgttgtact gctgaatcag tcgagtggca tccgagcccg
     4981 tttccatcgc gaagacgacg tcaacgccga gactggcaag ctgaccgata tacgggctga
     5041 agtcggaggt gttgatggga tgccatagct gagcca
//
LOCUS       DATCXU010000257         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_682539,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000257 DATCXU010000000
VERSION     DATCXU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682539"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="motB"
                     /locus_tag="VIR04_05760"
     CDS             complement(<1..937)
                     /gene="motB"
                     /locus_tag="VIR04_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY9460287.1"
                     /translation="MSKEDVGRVVIRRPHGKSERHHDDSWKIAYADFVTAMMSFFLVM
                     WLVSIVPKKELQGVAEYFRMPLMTAIVGGPKMDTSTTIIPGGSPSVIPNKDPLPQKSD
                     DDALKKARNSAQQEEDRNDTRRLEGLKSDIDTLIKTDPVLKEFKNQLLLDMTPDGLRV
                     EIVDKPPHPMFATGKAQVQPYMGKILRRLGPVFNKLPNKVTISGHTDAIQYATGEREY
                     SNWELSADRANSARKELVAGGMDEGKVKRILGLSSSVSLVKDNPNAAVNRRISLVVLN
                     KSAERRIDEQDAVGTSSAQLKDALSPSLPDPESEGA"
     gene            complement(950..1825)
                     /gene="motA"
                     /locus_tag="VIR04_05765"
     CDS             complement(950..1825)
                     /gene="motA"
                     /locus_tag="VIR04_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY9460288.1"
                     /translation="MLIFLGFLVVFVSVFGGYAAAGGHLGALYQPFEFLLIGGAALGA
                     YIASNSGKSVKLLIISIPIAFKSSPYSKALYMELMALLYVLLNKARRDGLMSVEADIE
                     EPGASAIFAEYPRVLNDARLVEFITDYLRLMISGNMSSFEIETLMDQEIEAFHNERSI
                     PANALRAVSDALPAFGIVAAVLGVIKALASVDQPAAVLADLIAKAMVGTFLGVLLAYG
                     FVAPLAATVERRTVGSTKVLECIKVTLLASMNNYPPQLAVEFGRKVLYSTVRPSFIEL
                     ENHVRQAKVLSNKKA"
     gene            complement(1970..2521)
                     /gene="flhC"
                     /locus_tag="VIR04_05770"
     CDS             complement(1970..2521)
                     /gene="flhC"
                     /locus_tag="VIR04_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEY9460289.1"
                     /translation="MATKSVAQEAEEILLATDMINLGARLQVLQAETALSYDRLARLY
                     REVKGCSPPKGMLPFSVDWFMTWLPNIHSSVFFSVYQFMDAHTPAKKAHALIEAYRVY
                     LEQSSAGRPAGSTEEPVLSFTRAWMLIRFFDSGMVQLSRCVRCKGRFIAHAHEPQQSF
                     VCSICRPPPRAGKTRALFASKLG"
     gene            complement(2552..2878)
                     /gene="flhD"
                     /locus_tag="VIR04_05775"
     CDS             complement(2552..2878)
                     /gene="flhD"
                     /locus_tag="VIR04_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEY9460290.1"
                     /translation="MSEATNSSLLGDIQEVNLSYLMLAQRLLRENYAAGMFRLGFSAD
                     VADIITQLSPSQLVKLAGSSSLICGFRLNDYELLSALTLDVLGGVLQQAHSTILLSQR
                     SPEQAL"
BASE COUNT          818 a          910 c          801 g          748 t
ORIGIN      
        1 cggcgccttc cgattcgggg tcgggcaagc tcggactgag agcatctttc aactgagcag
       61 aggacgttcc caccgcatcc tgctcgtcaa tacgacgttc ggccgattta ttcaacacca
      121 caagactgat acgacggtta acggccgcat tcggattatc tttaaccaaa ctgactgaag
      181 aagataaacc aagaatccgc ttgacctttc cttcgtccat acctccggcc accaactcct
      241 tgcgagctga attagccctg tcggcggaca gctcccaatt actgtattcg cgctcgccag
      301 tcgcatactg aatagcgtcc gtgtggcccg aaatggtcac tttattaggc aacttattaa
      361 aaaccggccc caatcgacgc aagatcttcc ccatgtatgg ctgcacctgc gcttttccgg
      421 tggcaaacat ggggtgaggc ggcttatcga caatttcgac acgcaagccg tcgggcgtca
      481 tatccagcag caattgattt ttgaattctt ttaggacggg atcggtcttg atcaacgtat
      541 cgatatcgct tttcaaaccc tccaggcgtc gtgtgtcgtt ccggtcctct tcttgctgcg
      601 cgctatttcg cgcctttttc agcgcgtcgt catcggactt ttgaggcagt ggatctttgt
      661 tgggaatgac gctgggagaa ccgcctggaa taatcgtcgt cgatgtatcc atcttgggac
      721 cgccaacaat cgccgtcatg agaggcatgc gaaagtattc ggccacccct tgcagttcct
      781 tcttgggtac gatcgacacc agccacatga ccaggaaaaa agacatcatt gccgtcacga
      841 aatcagcata agcaattttc caactgtcgt cgtgatgtct ctcggatttg ccgtgtgggc
      901 ggcggatcac aacacgaccc acgtcctctt tactcatagg tcgaacccgc tatgcctttt
      961 tattggacag cactttggcc tgccgcacgt gattttcaag ttcaataaac gacgggcgaa
     1021 cggtggaata caaaaccttg cggccaaatt ccaccgccaa ctggggcgga tagttgttca
     1081 tgctggccag caaagtcacc tttatgcact ccagaacttt ggttgatccg accgtgcgac
     1141 gttcgaccgt cgccgccaat ggcgcaacga aaccatacgc cagcagcacc cccagaaacg
     1201 tgcccaccat ggctttcgca atcaggtcgg ccagcaccgc cgccggctga tcgaccgacg
     1261 ccagcgcctt gatgacgccc agcaccgccg ccacaattcc gaacgcgggc aaggcatcgg
     1321 atacggctcg caacgcatta gcgggaatgc tgcgctcgtt atgaaacgct tcgatttcct
     1381 gatccataag cgtttcaatt tcgaacgagc tcatattgcc gcttatcatc aaacgcaaat
     1441 aatcggtaat aaactccacg agccgcgcat cgttcagaac ccggggatat tcggcaaaaa
     1501 ttgcgctcgc gcccggttcc tcgatatcgg cctcgaccga catcaggccg tcgcgccgcg
     1561 ccttgttcag caatacatac aaaagagcca tgagctccat atacagcgct ttgctgtaag
     1621 gcgaactctt gaaagcgatg ggaatcgata tgatgaggag cttgaccgat ttgccgctat
     1681 tggatgcgat ataggcgccc agcgcggctc cgccgatcag aaggaattca aagggttggt
     1741 atagtgcgcc caagtggccg cccgccgccg cgtaaccgcc aaacaccgag acgaaaacta
     1801 ccaggaatcc gaggaaaatt aacacgtgcg caacctttgt cgaacatatc tgacaatgat
     1861 cgctcttttc ggagactcca aaagcacgcg tcaatgggga tttttgaacc cagatcaggg
     1921 ctctgaaaat ggataccccc tttatgaacg atcaaagggg gtatgtctgt caacccagct
     1981 tggacgcaaa cagcgcccgg gttttccctg ctcggggagg aggccggcaa atagagcaga
     2041 caaagctctg ttggggttca tgcgcatgcg caataaatcg gcctttgcaa cgcacgcaac
     2101 gcgacaactg caccatgccg ctgtcgaaaa aacggatcag catccaggcg cgcgtaaagc
     2161 tcaggacggg ttcctcggtc gagcctgccg gccgccctgc cgaagactgt tcaagataaa
     2221 cgcgataagc ctcgataaga gcatgtgctt tcttggcggg ggtgtgcgcg tccataaact
     2281 ggtatacgct gaaaaacaca ctcgaatgaa tattcggcag ccaggtcatg aaccaatcca
     2341 ccgaaaaagg gagcattccc ttcggtggcg aacacccttt gacttcacga tatagccgag
     2401 ccagacggtc gtaacttaat gcagtctcgg cctgcaatac ttgtagcctt gcccccaggt
     2461 taatcatatc ggtagccaag agtatttctt cagcctcttg tgccacactt ttcgttgcca
     2521 tggtgctcgc cctccccggg cattagtttt tttataaagc ctgctctgga gagcgttgcg
     2581 acaggagtat ggttgaatgc gcttgctgca atacgccgcc caggacatcc agtgtcaagg
     2641 ccgacagcaa ctcgtaatca ttcaaacgga aaccgcagat caaagagctt gatcccgcca
     2701 gtttgaccag ttgagaaggg gataattgag tgatgatatc ggcgacgtcg gcgctaaagc
     2761 ccaaccgaaa catgcctgca gcatagtttt cgcgcaatag ccgctgcgcg agcatcaggt
     2821 atgacaaatt tacttcctga atgtcgccta ataatgaact gttagtagct tccgacataa
     2881 cggtatgtct ccccttggag caatgaattt caacgaacca acgaaaaaat tcagtcgatt
     2941 tcagatgaaa caaaaatacc ttgtcagttt aacaaagttt cagttttgta tctgaaaggc
     3001 tcaaataatc tcgaattact gattttgata cttacggctg caatagtacg gcttatttaa
     3061 gattcataca gcgcttcaaa aaacgttaca tttttgtgcg cgaaaaacaa cggtgtgtaa
     3121 cgaactaatc gcccataatc gcgttatgaa cacaatcgcc gcacaccgac tcgcgcgttt
     3181 tctgtttttc gcggccactt tggcaatagt ggcgggatgc gcggggaccg gacgccgcat
     3241 gggccagctc ggtcagaatg ccaattcacg cccgccg
//
LOCUS       DATCXU010000258         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_179878,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000258 DATCXU010000000
VERSION     DATCXU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179878"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIR04_05780"
     CDS             <1..251
                     /locus_tag="VIR04_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460291.1"
                     /translation="AGSIRSLHEAGLWNYGQGVVFDLSAVLPVDSVFGAVFAGVFGYN
                     DAPTASEAIAYVGFLAVSSMLFWAPLRHMKPASLSTGI"
     gene            257..1444
                     /gene="efeO"
                     /locus_tag="VIR04_05785"
     CDS             257..1444
                     /gene="efeO"
                     /locus_tag="VIR04_05785"
                     /inference="COORDINATES: protein motif:HMM:NF007697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEY9460292.1"
                     /translation="MASRQRSAAPVGASSRLIRLGVWASAVLVVLAAGALYFVSTFAH
                     NRKQAAGAVVVAITGRTCDPGQLTVSAGRTTFQIANHSDRVVEWEILDGVMVVAEREN
                     IAPGFTQSLSANLEPGDYSMTCGLLGNPRGQLHVTPSKVAVTGVPHPLPRAYIGPLAE
                     YRVYLMREAGQLAPAVGGLADAIAQGNLPLSRSLYISAHRIYMHIEPVAEMFADLDQR
                     IDAQANDFKRRERDPSFTGFHRIESGLFAQDSIGGIAPVARQLVADVGALQERVRTLK
                     TPPERLSRGAARSMQKLAEGITDGASANGAVALSDARSRVDGVVETVGLLRPLLIRAD
                     AALQARIDADLKNVQAELDRMQPSAMDRSAHHDGNRPPLVAQLKTLATDLAAVNTRLG
                     LIE"
     gene            1456..2742
                     /gene="efeB"
                     /locus_tag="VIR04_05790"
     CDS             1456..2742
                     /gene="efeB"
                     /locus_tag="VIR04_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEY9460293.1"
                     /translation="MNDTEISSPQRRRMLQRLGAAGGAIATCGIAQAQQQASGNVAQA
                     PESGQINERRDFYGRYQTGVITPRPATGMIASFDVIASDRKELERLFRLLTQRIAFLM
                     AGGEAPTLDSKFPPPDSGILGPVITPDALTITVSVGHSLFDDRYGLRSLEPIALQRMT
                     GFPNDALVPALCHGDVSLQFCANTMDTNVHALRDILKNLSDLLVLRWKQEGTVPVKAV
                     RHGEPVESARNFLGFRDGSANPDTRDDALMERVVWVEGKSTEPAWAVNGTYQAVRIIR
                     NFVERWDRTPLHEQEAIFGRVKDTGAPFDGKLESDVPHYGADPKGRVTRLDAHIRLAN
                     PRTAATQRNLVLRRPFNYSNGVTKSGQLEMGLLMILYQANLNDGFIAVQKRLNGEPLE
                     EYIKPIGGGYFFTLPGVRDRHDYLGRALLQAANAQI"
     gene            2778..3605
                     /gene="efeO"
                     /locus_tag="VIR04_05795"
     CDS             2778..3605
                     /gene="efeO"
                     /locus_tag="VIR04_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEY9460294.1"
                     /translation="MKRILSAIAGVWVLLGGAICLPANAAISPLDLVNPVADYKIYVS
                     EKLDVLVRDTKTFTDAIKAGDLAAAKKQYAPTRVSYETIEPIAELFSDLDSTIDSRAD
                     DHEKREADPGFIGFHRLEYGLFAKNSTNGLGPIADKLMADILDLRKRVAGLAFPPERV
                     VGGAAALLEEVAATKVSGEEDRYSHTDLWDFQANIDGAKKIVDLFRPLIEGENEGFFK
                     KVDANFATVDRILAKYKTPDGFKAYDSVTDRDRVALIGPVNTLAEDLSDLRGLLDLN"
     gene            3644..>4091
                     /locus_tag="VIR04_05800"
     CDS             3644..>4091
                     /locus_tag="VIR04_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460295.1"
                     /translation="MARAQHHDHVWLIKSLLLVTVAALHAALFAGILASARVTLREAP
                     EPVDVRFVEIAPDIGQQTPAPALATVPPPELQPESQPASEPEAAPEPAPEPETALPPP
                     PEPPAPDAITLPPPEIAPELVAQPKPEPEPETRPKPKPKPKAKPRPK"
BASE COUNT          820 a         1119 c         1222 g          930 t
ORIGIN      
        1 tggccggctc gatccggtcg ctgcacgagg ccggattgtg gaattacggg cagggcgtcg
       61 tcttcgattt aagcgcagtg ctgcccgtgg acagcgtgtt tggcgcagtc tttgcgggtg
      121 tttttggtta taacgatgcg cccacggcaa gcgaggcgat tgcatatgtc ggatttttag
      181 cggtttcgtc catgctgttt tgggcgccgc tgcggcacat gaaaccggcg tctttgtcga
      241 ccgggatttg accacgatgg cgtccagaca acgatctgct gcgcccgttg gcgcgtcttc
      301 ccggctaatt cgtttaggag tatgggcttc agccgttctg gttgttctgg cggccggcgc
      361 cctttatttt gtatcgacct tcgcgcataa ccgaaagcag gcagccggcg ctgtagttgt
      421 cgcgatcact ggccgaactt gcgatccggg tcagcttacg gtttcggccg gacgaacaac
      481 ttttcaaatt gcgaaccatt cggatcgcgt tgttgaatgg gagatactcg acggcgtgat
      541 ggtggtcgcg gagcgtgaga atatcgcccc tggcttcacg caaagcctga gcgcgaacct
      601 tgaacccggc gactattcca tgacctgcgg tctgcttggc aatccgcgcg gccaactgca
      661 tgtgacgccg tccaaggtgg ccgtgactgg agtgccgcac cctttgccgc gcgcttatat
      721 tggcccattg gctgaatatc gcgtgtatct gatgcgtgaa gctggccagt tggcaccggc
      781 cgttggcggc cttgcagacg cgattgctca gggtaatttg ccgctttcca ggtcccttta
      841 tatttccgcg catcgtattt atatgcacat tgagccagtc gctgagatgt ttgctgacct
      901 ggatcagcgc atcgatgcgc aggccaatga ctttaaacgg cgtgagcggg atccttcttt
      961 cacaggcttt catcgtattg aaagcgggct tttcgcacag gattcgattg gcggcattgc
     1021 gcctgtggcc aggcagttgg tggccgatgt gggtgcgttg caggagcgtg tgcgcacatt
     1081 gaaaacccct ccggaacggc tctcgcgcgg cgcggcgcgt tcgatgcaaa aattggcgga
     1141 agggatcacc gacggcgcaa gcgccaatgg cgcggtcgct ttatccgatg cgcgaagccg
     1201 cgtggacgga gtggttgaaa cagtcggttt gcttcgcccc ttattgatca gagcggatgc
     1261 tgccttgcag gcgcgcattg atgccgatct gaaaaatgtt caggcagaac tggaccggat
     1321 gcagccgtcg gcaatggatc gcagcgcaca tcacgatggc aatcgtccgc cgttagttgc
     1381 acagctcaag acgttggcga cggatcttgc cgcggttaat acacgcctcg gcttaattga
     1441 atgaaggtga cggttatgaa cgatactgaa atctcttcgc cgcaacgccg cagaatgttg
     1501 cagcggctcg gcgccgccgg cggcgccatt gccacctgcg gcattgcgca ggcgcagcag
     1561 caagcgtcgg gcaacgtcgc ccaggctccc gagagcggcc agatcaatga gcggcgtgat
     1621 ttctacggcc ggtatcaaac gggagttatt acgccccggc ccgctaccgg aatgattgcg
     1681 tcgttcgacg tgattgcttc tgatcgcaag gaactggaac ggcttttccg tctgctgacg
     1741 cagcgtatcg catttttgat ggcggggggc gaagcgccga ctctggattc caaatttccg
     1801 cctccggatt caggtatttt gggccccgtc attacgcccg atgcgttgac cattaccgta
     1861 tcggtgggcc attcgttgtt cgatgaccgc tatggcctga ggtcgctcga accgattgcg
     1921 ttgcagcgta tgaccggttt tccaaacgat gctctggtgc cggcattgtg ccatggcgat
     1981 gtatcgctgc agttttgcgc gaacaccatg gacaccaatg tgcacgcctt gcgtgacatc
     2041 ttgaagaatt tatctgatct tttggtgttg cgctggaagc aggaaggcac ggtgccggtt
     2101 aaggcagtgc gtcacggcga accggttgaa agcgcgcgca atttcctggg tttccgggac
     2161 ggctccgcca atccggatac gcgtgatgat gccttgatgg agcgggtcgt ctgggtcgaa
     2221 gggaaatcca ccgaacccgc atgggccgta aatggcacgt atcaggctgt gcgtattatt
     2281 cgaaattttg tggaacgctg ggaccgtacg ccgttgcacg agcaagaagc catattcggc
     2341 cgcgttaagg acaccggggc tccctttgat ggcaagctcg aatcggatgt tcctcattat
     2401 ggggccgacc ccaagggcag ggtgacgcgc ctggacgctc atatcaggct ggccaacccg
     2461 cgaacggcgg cgacccagcg caacctcgtt ttgcgccggc ctttcaacta ctcgaatggg
     2521 gtgacaaaat ccgggcagct tgaaatgggt ctgctaatga ttctttacca ggccaatttg
     2581 aatgatggct ttatcgctgt gcaaaagcgc ttaaacgggg agccgctgga ggaatacatc
     2641 aagccaatcg ggggcggtta ctttttcacc ctgccgggtg tgcgcgatcg gcacgactac
     2701 ctgggccgcg ctttgctgca agcggccaac gctcagatct gattttctac tggccgacaa
     2761 ataggaaaaa tttattcatg aaacgaattc ttagtgcaat tgcaggtgtg tgggtgctgc
     2821 ttggcggcgc gatttgtctg cctgccaacg cggcgatatc gccgcttgat ctggtgaatc
     2881 ccgttgccga ctataagatt tacgtctctg aaaagttgga cgttctggtg cgtgatacga
     2941 aaacgtttac cgacgccatc aaggccggcg accttgccgc cgcgaaaaaa cagtacgctc
     3001 ccacccgcgt gtcctacgaa acgattgagc ctatcgcgga attgttcagc gatctggaca
     3061 gcactattga ctcacgcgct gatgatcatg aaaaaaggga agcggatccc ggttttattg
     3121 gttttcaccg tctggaatat ggcttgttcg ccaagaacag cacaaatggc ctggggccca
     3181 ttgccgacaa attgatggct gacatccttg atctgcgcaa gcgcgtcgca gggcttgcat
     3241 ttccccccga gcgagtggtg gggggagccg ccgcgctgct ggaagaagtt gccgcgacga
     3301 aggtgtctgg agaggaggat cgatacagcc ataccgatct ttgggatttt caggccaata
     3361 ttgatggtgc gaagaaaatc gttgatttgt ttcgtccatt gattgaaggc gaaaacgaag
     3421 gcttttttaa gaaagtcgat gcaaactttg ctacagttga ccggatcctg gccaaataca
     3481 aaacgccgga tggcttcaag gcttacgata gcgtcacaga ccgcgatcgc gttgcgttaa
     3541 ttggtccggt caatacactg gctgaggatc tgtccgattt gcgcggcctg ctggacctga
     3601 actgaactag atccaagttg aaacttctca cattgatgga ttcatggcac gcgcccaaca
     3661 tcacgatcat gtctggctaa tcaagtcctt gttactcgtc actgtggcgg cgctgcatgc
     3721 ggcgctgttt gcgggtattt tggcctcggc ccgtgtgaca ttgcgcgaag cgcccgaacc
     3781 ggtcgatgtg cggttcgttg aaatcgcgcc ggatattggc cagcagacgc cggctcccgc
     3841 gttggcaacg gttcctccgc cggaattgca gccagagtcg caaccggcct cagaacccga
     3901 ggcggcgccc gagcctgcgc cagagcccga aactgcgctc ccacccccgc ctgagccgcc
     3961 tgcgcccgat gccattaccc tgcctcctcc cgagattgct cccgagctgg ttgcgcagcc
     4021 gaagcccgag cccgagcccg agaccagacc aaaacccaaa ccaaagccca aggcgaaacc
     4081 caggccgaaa c
//
LOCUS       DATCXU010000259         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1116997,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000259 DATCXU010000000
VERSION     DATCXU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116997"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VIR04_05805"
     CDS             <1..1209
                     /locus_tag="VIR04_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460296.1"
                     /translation="MAVAPALKGADEALVVGSKIMLPAYAAAMVNAMLAHADETDDSH
                     EKSKFHPGCAIVPSALALGQLRAASGSDIIRAVALGYDVGARVLEALGPMPLNMAGHA
                     SHAIGPLFGCGAVAGYLMGFDARRNRHLLSYLGHETSGLACWMTDTDHIQKAYVFGAM
                     AAKNALFAALLCQHGWTGCNEVVSIERGLLEAYGNPEHGRTLEEPLELGAEILHSDIK
                     KWSVGSPIQAPLDSLEVMLDSIPVDPAQIAAIEVEIQANEVFIVQARNMPNISLQHLL
                     ALYLVDRRLSFESVHDAARMNDAAVLALRSRVKLIPSLELQRSGGRQAIVRVILTDGK
                     VLSHHTPRVRGTWENAMTRDEVHAKALELIEPVMGGPATAALLSGLWRLETLRAPEWV
                     DLVKSTAIRI"
     gene            1246..2235
                     /locus_tag="VIR04_05810"
     CDS             1246..2235
                     /locus_tag="VIR04_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY9460297.1"
                     /translation="MQAYWIKTTDGQSIVELRDTPKPVPKPHQVLVRVRASGLNRGEF
                     IRNNGLHGQSGAAKAVGNEAAGEVVELGADARGVKLGDRVMGRSPGAFAEYAVMDARE
                     AIPVPPNLSWEQAAAIPLVFMVVYDMLVAQGHVAANEWLLVTGISSGVGVGALQMAKA
                     LGVHVIGTSGSASKLKRLEAYGLDVGLCTRNADFSDAVMGATGGKGVNLVVNAVGGSM
                     FAECVRSMAYEGRFATVGYVDGVLKSEIDIAALHTKRLTLFGVSNKLRSAEQRIALVA
                     GFVADILPMFADGRIKPLIDKVYPFSQLEAAKDYMEANLHVGKIVVSIPGQEA"
     gene            2301..>2864
                     /locus_tag="VIR04_05815"
     CDS             2301..>2864
                     /locus_tag="VIR04_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460298.1"
                     /translation="MSTTAAIQNLNEFHETEGLTREVAQFVLDTDMQDLPPDLIDLGK
                     KSILDGIGLALAGSVAKSGELVTAYLKELDVKGGATAIGTALRLPPRFAAFANGIAIH
                     ADDFDDTQLAIGKDRVYGLLTHPTAPALPAALSVAETLGKDGRQTMAAYHIGVEIECK
                     IAEAINPRHYQHGFHATATCGTFAAAAA"
BASE COUNT          590 a          752 c          893 g          629 t
ORIGIN      
        1 atggctgttg caccggcgtt gaaaggcgcc gatgaagcgc tcgtcgttgg ctccaaaatc
       61 atgcttcccg cgtacgccgc ggcgatggtc aatgccatgc tcgcgcatgc cgatgaaacg
      121 gacgattcac acgagaaatc aaagtttcat cccggctgcg caatcgttcc gtccgctctg
      181 gcgctgggac agctacgggc cgcgtcgggc tccgacatta ttcgcgcggt ggcgcttggg
      241 tacgatgttg gcgcgcgcgt actcgaagcg ctgggtccca tgccgcttaa tatggccggg
      301 catgcctcgc atgcgatcgg cccgctgttc ggttgcggcg cggtagccgg ctatctgatg
      361 ggcttcgatg cgcggcgcaa tcggcatttg ttgtcttatc tgggacatga aacatccggc
      421 ctggcttgct ggatgacgga tacggatcat attcaaaaag cttatgtgtt tggtgcgatg
      481 gcggccaaga atgcgctatt tgcagcgctc ctgtgtcagc acggctggac gggctgcaat
      541 gaagtagtct caattgagcg cgggttgctt gaagcgtatg gtaatccgga gcatggccgc
      601 acgctggaag agccgcttga actaggcgcc gagatactgc attccgatat caagaaatgg
      661 tccgtcggct cgccgattca ggctccgctt gacagtctcg aggtcatgct ggactccatt
      721 ccggtagacc cggcgcaaat cgccgctatc gaagtcgaaa ttcaggcgaa cgaagtcttc
      781 atcgttcagg cgcggaatat gcccaatatt tcactgcagc atttgttggc gctgtatctg
      841 gttgaccggc ggctgagctt cgagtcggtg catgatgccg cgcgcatgaa cgatgcggcg
      901 gtgctggcgc ttcggtcccg tgtcaagttg attccaagtc ttgagttgca acggtctggc
      961 ggtcgccagg cgattgttcg cgttatcttg acggatggca aggtccttag ccaccacact
     1021 ccgcgcgtgc gcggcacttg ggagaacgcc atgacgcgcg atgaagttca cgccaaggcg
     1081 ctggaactga tcgaacctgt catgggagga ccggcaacgg cggcattgtt aagcggttta
     1141 tggcggctcg aaacgttgag ggcgcccgaa tgggtagatc tcgttaaaag cacggcaatt
     1201 cgaatttaac cgggttgatt tgaagttata aaaaggaaga gtgcaatgca ggcatattgg
     1261 attaagacaa cggatggcca atcaattgtc gaacttcggg acacacccaa acccgtcccc
     1321 aaaccccatc aagtcctggt gcgagtgcgg gcatcgggat tgaatcgcgg cgaattcatc
     1381 cgcaacaacg gtttgcatgg tcaatccggg gcggccaagg cagtgggaaa cgaggcggcc
     1441 ggcgaagttg ttgagctggg cgccgatgcg cggggcgtga agctgggcga ccgggttatg
     1501 ggacgcagtc cgggagcctt cgccgagtac gccgtgatgg atgcgcgtga agccataccc
     1561 gtcccgccta atctttcgtg ggaacaggcg gccgccatcc ccttggtatt catggtggtg
     1621 tacgacatgc tggtcgctca gggccacgtt gccgccaatg aatggctgct ggtgacgggc
     1681 atatcgtcag gcgttggcgt tggcgcgctg caaatggcca aggcattggg cgtccatgtg
     1741 attggcacat cagggtccgc aagcaagctc aagcgcctgg aggcgtatgg tcttgatgtc
     1801 ggcttgtgca cgcgcaacgc cgatttctcc gacgccgtaa tgggggcaac gggtggcaag
     1861 ggcgttaatc ttgtggtcaa cgcggttggc ggatcgatgt ttgccgaatg tgtgcggtcg
     1921 atggcgtatg aaggccggtt tgccacggtt ggctatgtcg atggcgtact gaaaagcgaa
     1981 atcgacattg ccgccctgca caccaaacgc ctgaccctgt ttggagtttc gaacaagctg
     2041 cgtagcgccg aacaacgcat tgcgttagtg gctggattcg tcgccgatat actgccgatg
     2101 tttgccgacg gccgcatcaa gccgctgatt gacaaggttt atccattttc tcaactagag
     2161 gccgccaaag actacatgga ggcgaatctt catgtgggca agatcgtggt ttccattcct
     2221 ggccaggagg cctgaacgat agccgtccat attgcaatgg cggctgagtt ttacctattt
     2281 tttttgagac atagaacgtc atgagtacga ccgcagcaat acagaatttg aacgagttcc
     2341 acgaaacgga ggggcttacc cgtgaagtgg cgcaatttgt tctggatacc gatatgcagg
     2401 atttgccccc ggatctgatt gatctgggca aaaaatccat actggacggc atcggattgg
     2461 cattggcggg gtcggtcgcc aaaagcggag agctggtgac tgcgtatctg aaagaactgg
     2521 atgtcaaagg cggagccacg gccattggca cagcactgcg cttgcctccg cggtttgcgg
     2581 cgtttgccaa cggcatcgcc attcatgccg atgattttga cgatacccaa ctggcaatcg
     2641 gcaaagaccg cgtttatggg ctgttgacgc atcccacagc gccggctctg ccggcggccc
     2701 tctcggttgc cgaaaccttg ggcaaggatg gccggcagac catggcggct tatcacatcg
     2761 gtgttgagat cgagtgcaaa atcgccgagg cgataaaccc gcgccattat caacatggtt
     2821 ttcacgccac tgccacatgc ggaaccttcg ctgcggctgc ggcc
//
LOCUS       DATCXU010000260         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_868326,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000260 DATCXU010000000
VERSION     DATCXU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868326"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1369)
                     /locus_tag="VIR04_05820"
     CDS             complement(14..1369)
                     /locus_tag="VIR04_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460299.1"
                     /translation="MGQNLTRDFARMLQSWPAEDAGLDAVARLLLLDGLAVARAGAVE
                     LGPRLAGRMARQYGSHPASVVIGQGFSAAPAQAAQINGMAMHVLDYEPMWNPPNHAMS
                     TILPGLLALAQVRESEDDAPQGARVLRALCKGIEAQGRLRLASGQLEPKDLTLHPPGV
                     IGPIASAVACGDLLDLDQEQLVMAIGIAASRACGVLANVGSMTKALHCGDACRSGLEA
                     ATLALSGFTADGDALGGPRGYGQAYFGDRFDPAALLAPLVTPRVLDPGPAWKLFPSQY
                     ATHFAITAALDCRTAMGGAAAIRDVKITTPVMPYIDRPNPNSGLDGKFSYQYCVVAAL
                     LDGRINLATFSDQRRFSPDVAALLEHTVLSQTSAISGRFDAMHVDVSVTLVDGTQTRR
                     RCAAPLGSWSRPIEPIVIEAKAHDLLDGLLDEQRANAFWSAMAMPTDVLRISDLMPGL
                     A"
     gene            complement(1379..2881)
                     /gene="tcuA"
                     /locus_tag="VIR04_05825"
     CDS             complement(1379..2881)
                     /gene="tcuA"
                     /locus_tag="VIR04_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY9460300.1"
                     /translation="MAEKSIAHQNDAGEWDVIVVGAGNAALAAAVSAHENGAARVLVL
                     EKARREDRGGNTHFSGGLLRFAFDNTEQILEVSPEADRLEGFIGGVEPYPESAFLADL
                     MRMTGDRADQGLAKILIGNSFDTIKWMSRLGIVMEPAVSLGGIVVDGKIKWPKGAVIR
                     ALHEGVGLSKSWFKIAEREGVEIRYGAAARELVQDAKGRVVGVMVQDTEGLREYRGRA
                     IVLACGGFEANSAWRAKYLGQPWDHAKVRGSRHNQGDGLRMALEIGAMPIGQWSGCHS
                     TPINAQSPDYGVLELTDKTNRLSYPYGVMINRDGVRFLDEAPDFQFFTYAKYGRIILK
                     QPDSVAYQIFDQKVVHLLEGRYSTSEPIVGDTLDALIDKLPVNRQVAKRTLAAYNAAA
                     GHGSFNPGVLDRMCTKDLELPKSNWAQKLDAGPFVCYPVTGGITFSFGGLKIDDQGRV
                     LDSGWRPIEGLFTCGEMVGNLFHDNYPGGTGLTSGAVFGRLAGRSAARLA"
     gene            2982..3167
                     /locus_tag="VIR04_05830"
     CDS             2982..3167
                     /locus_tag="VIR04_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460301.1"
                     /translation="MLARTQMLHLDFPGRMCREGNMAFPFSPSDQEAGAVYKFSMHHV
                     LAPSDPCELFPIDYETI"
     gene            3179..3517
                     /locus_tag="VIR04_05835"
     CDS             3179..3517
                     /locus_tag="VIR04_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEY9460302.1"
                     /translation="MPLIKDIATVCKSKNAGPFDLTIDVVFDNAEIYQKVKDTGVLCA
                     RLFARLYKVRESDVLFTPYDSAYAFKATLPRQVPAGGIGDTDVYGAQQHAPVLAVNIP
                     LPEVPGNKNA"
     gene            complement(3522..4280)
                     /locus_tag="VIR04_05840"
     CDS             complement(3522..4280)
                     /locus_tag="VIR04_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460303.1"
                     /translation="MSSSSLSDVRRALLPAGKLRAAINYGNIVLAQKDAVTGAPGGVS
                     VALARELARRLGVELEILGFDAAGKVFAETDNNTWDIGFLAIDPVRGQKVLFTAPYVL
                     IEGTYLVNRNSPLRSIDDIDAEGVRVAVGKGAAYDLFLTRELKKAEIVRADTSAAAID
                     LFLEKNLDAAAGVRQPLEKAAEGNARLKVLPGSFTSIRQAMVTPKKNQIALDYLHAFI
                     EEMKASGFVERELKTSGQDSAGIAPPENQAPQPV"
     gene            complement(4315..5469)
                     /locus_tag="VIR04_05845"
     CDS             complement(4315..5469)
                     /locus_tag="VIR04_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEY9460304.1"
                     /translation="MTEANDISRLRAVFMRGGTSKGLILHSRDLPSRREQWDSLFLAL
                     MGSPDIHGRQLNGMGGGISSLSKVCIVGPSTRPDADVDYTFAQVMVNEARVDYSGNCG
                     NMSSAIGPFAIDEKLVKATDGDVLVRIHNTNTSKVIHAVFPVKNGRACVGGDLEIPGV
                     SGKGAKVRLEFLDPGGATTGTLLPTGHEAQLLDVPGLGKINVSMVDAANACVFVNAQD
                     LGLAGTELPAILNERPDVLKKLNDIRIHASVAMGITPDLEQARAKRVVPFVGFVSPPQ
                     TATTLSGATIRAEDIDLTARMISSGQPHMALQLTASLCLAVAAEIKGTVVSKAARAAT
                     DGTLRIGMPSGILTVGAEVDFEGQQWNARRGYFYRTARRLFDGHVYPAQS"
     gene            complement(5521..6687)
                     /gene="tcuB"
                     /locus_tag="VIR04_05850"
     CDS             complement(5521..6687)
                     /gene="tcuB"
                     /locus_tag="VIR04_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEY9460305.1"
                     /translation="MMETLPVSIQKAHTAGIEALMLTDDEKEVDRVMQICNACRYCEG
                     FCAVFPAMTHRLDFVKSDINYLANLCHNCGACLHSCQYAEPHEFAVNVPRAMARVRMQ
                     TYVEYAWPARFGALYKSAGLTTALAVGAGLALFLILVLIINGSLWHAPLAGNFYAIFP
                     HNTLVLMFGSVFAFAVLALAIGGVRFWRNISPPESLKNRTQAIASATEATKDVLTLRY
                     LGGGHGEGCNNEDDRFTLWRRRFHHLTFYGFIFCFAATSIATIYHYFFAWYAPYPVIS
                     VPVIFGIIGGIGLFIGPVGLLWMNVRRNPEHGDANQKPMDRGFIALLFLTGLTGLALL
                     ILRDTGGMALLLAIHLGFVMAMFVTMPYGKFAHGVYRAAALLKYALEKRQMGSV"
     gene            complement(6701..8098)
                     /gene="tcuA"
                     /locus_tag="VIR04_05855"
     CDS             complement(6701..8098)
                     /gene="tcuA"
                     /locus_tag="VIR04_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY9460306.1"
                     /translation="MIDVLVIGGGNAALCAALMAREAGASVLMLESSPKEWRGGNSQH
                     TRNLRCMHDEPQDVLSGSYPEEEFWQDLLKVTGGRTNEHLARLVIRASSSCRGWMRQH
                     GVHFQPPLTGALHVARTNAFFMGGGKALVNAYYRSAQNLGVDIRYNSPVDSLELDDGR
                     FIAARLGDERIQAKACVLACGGFESNRKWLREAWGQINGEWVADNFLIRGTRYNMGVL
                     LKFLLDAGADSIGEPSQSHCVAIDARAPLYDGGICTRIDCVSLGVVVNRQATRFYDEG
                     EDFWPKRYAIWGRLVARQPGQIAYSIIDSKAVGRFMPPVFPGAKANTLSELAQQLELD
                     PTAFVRTIDAYNAACHVGTFNHAVLDDCHTEGIEPVKTHWAMPIDTPPFFGYALRPGI
                     TFTYLGVKVNENASVHFNGEPSPNLFAAGEMMAGNVLGQGYTAGVGMSIGTAFGRIAG
                     TRAALAAKSGAEITA"
BASE COUNT         1939 a         2455 c         2117 g         1698 t
ORIGIN      
        1 aaaagggcgg ccgtcaagcc aggccaggca tcaagtcgga aatgcgcaag acgtccgttg
       61 gcatggccat tgcgctccag aaagcattgg cgcgctgctc gtccagcaaa ccatcaagca
      121 ggtcgtgcgc tttggcctca ataacaatgg gctcgatggg cctcgaccag ctacccagcg
      181 gtgcggcgca acggcgcctg gtttgcgtgc cgtcaaccag tgtcaccgac acatcgacat
      241 gcatcgcgtc aaagcggcct gaaatggcag aggtctgaga caagacggtg tgctcaagca
      301 gcgcggcaac atccggcgaa aaacgtcttt gatcggagaa cgtcgcgaga ttaatgcggc
      361 cgtccaataa agccgcaacg acacaatatt gataagaaaa tttgccatcg agacctgaat
      421 tgggattcgg ccggtcgata taaggcatta caggcgtagt gattttgaca tcgcgtatgg
      481 ccgccgcgcc gcccatcgca gtgcgacagt cgagcgccgc agtgatcgca aaatgcgtcg
      541 cgtattgcga cggaaataat ttccaggcgg gaccggggtc gagcacgcgc ggcgtcacta
      601 acggagcaag caacgccgcc ggatcaaagc ggtcgccgaa ataggcctga ccgtaaccgc
      661 gcggccctcc taacgcatcg ccatcagcgg taaaacccga caaggccagt gtagcggctt
      721 caaggccgct acggcatgca tccccgcaat gcaatgcttt cgtcatcgag cccacattcg
      781 ccaatacgcc gcacgcgcgc gaagcggcga tgccaatagc catgaccagt tgctcctgat
      841 ccagatcaag taaatcgccg cacgccacgg cagaggcaat cggtcctatt acccccggag
      901 gatgcaatgt cagatcttta ggttcaagct gtccggatgc aagtcgcagt cgcccctgag
      961 cctcgatgcc cttgcacaat gcgcgcagta cgcgcgcacc ctgtggcgcg tcatcttcgg
     1021 attcgcgcac ttgggccaac gcgagcaggc ccggcaaaat ggtcgacatc gcatgattgg
     1081 gcggattcca cattggctcg taatcgagca catgcattgc catgccattg atctgcgccg
     1141 cctgagccgg cgccgcggaa aagccctgcc cgataacgac cgaagcagga tggctgccat
     1201 actggcgcgc catcctgccc gctaagcgcg gtcccagttc gaccgccccg gcccttgcca
     1261 cagccaggcc atcaagtaat agaagacggg ccaccgcatc cagtccggca tcttcggccg
     1321 gccaggactg caacatacgc gcaaagtcgc gcgttaaatt ctgacccatt tgatcgtgtc
     1381 aggccagacg tgccgccgaa cgcccggcaa ggcgtccgaa aaccgcccct gaagtcaagc
     1441 ctgtgccgcc agggtagttg tcgtggaaca gatttcccac catctcgccg caggtgaaaa
     1501 gcccttcgat aggacgccag ccgctatcaa gcacgcgccc ctggtcgtcg attttcagac
     1561 caccgaacga gaaagtaatg ccgcccgtaa ccggatagca gacgaagggt cccgcatcga
     1621 gtttttgcgc ccaattcgat ttcggcaatt cgagatcctt agtgcacatg cggtcaagga
     1681 cgccgggatt gaacgagccg tggcctgccg cggcgttata agcggccagc gtgcgtttgg
     1741 caacctggcg attaaccggc aacttgtcaa taagcgcgtc gagagtgtca ccgactatgg
     1801 gctcactggt ggaataacgc ccctcgagca gatgaaccac tttctggtca aaaatctgat
     1861 aagccactga atcgggctgc ttaagaataa tacggccata cttggcatag gtgaaaaact
     1921 gaaaatcagg cgcttcgtca aggaaccgca caccgtcccg attaatcatc accccgtacg
     1981 gataggataa acgattagtt ttatcggtca gttccaacac cccgtagtcc gggctttgcg
     2041 cgttgattgg cgtggaatgg caaccgctcc attggcctat cggcatggcg ccaatttcca
     2101 gcgccatacg cagaccgtcg ccctgattat ggcgcgagcc acgcaccttg gcgtgatccc
     2161 acggttgccc aagatacttt gcgcgccacg ctgaattggc ctcgaagccg ccgcacgcca
     2221 atacgattgc ccggccacga tattcgcgca gcccttcagt gtcctgaacc attacgccaa
     2281 caacgcggcc cttggcatcc tgaaccagtt cgcgcgcagc cgccccgtaa cgaatctcca
     2341 cgccctctcg ttcggcaatt ttgaaccatg atttcgatag cccgacccct tcgtgcaagg
     2401 cacgaatcac cgcgcccttg ggccatttta ttttgccatc gacgacgatc ccgccaagcg
     2461 atacggccgg ctccatgaca atgcccagcc gggacatcca tttgatcgtg tcaaacgaat
     2521 tgccgatcaa aattttggca agaccctggt cagcgcgatc gcccgtcatg cgcatcaggt
     2581 ccgccagaaa cgccgactcg ggataaggtt ccacaccgcc gataaaacct tcaagccggt
     2641 ccgcctcggg cgatacttca agtatctgct ctgtgttgtc aaaggcgaag cgcaacagcc
     2701 ctccgctgaa atgcgtattg ccgccccgat cttcgcgcct ggccttttcg agcaccagca
     2761 cacgagccgc gccattttcg tgagccgaca cggcggctgc aagcgcggca ttaccagcgc
     2821 ccacgacaat aacatcccat tcaccagcgt cattctggtg cgcgatactt ttttcggcca
     2881 tatcgagtct cctaagcttg catcgggtta tttacacaac agccttattt tgtaagtatg
     2941 cacagttatg cgattgtatg caacaattat ccggcagagt tatgctggca cgcacccaga
     3001 tgttgcatct ggattttccc gggcgaatgt gccgtgaagg gaatatggcg tttccgtttt
     3061 ccccgtcgga ccaggaggcg ggggccgtct acaaattctc gatgcaccat gtgctggcgc
     3121 cgtctgatcc ctgcgaattg ttcccgattg attacgaaac catatagtcg aattgaccat
     3181 gcctttaatc aaagatatcg cgacggtctg caaaagcaag aacgcgggcc cgttcgatct
     3241 tactatcgac gtcgtgttcg ataacgccga aatatatcaa aaagtgaaag ataccggcgt
     3301 cctctgcgca cgactttttg cgcggcttta taaagttcgg gaaagcgatg ttttatttac
     3361 gccctacgac tctgcatatg catttaaagc cacactgcca agacaggtgc ccgcgggcgg
     3421 cataggcgat accgacgtgt acggcgccca gcaacatgcg cctgtacttg ccgtaaacat
     3481 tccattgccc gaggttccgg gcaataaaaa cgcttaactg ttcatactgg ctgcggcgcc
     3541 tgattttcag gcggagcgat gccggcgctg tcctgaccgc tagtcttgag ttcgcgttca
     3601 acaaagccag acgccttcat ctcttcgata aaagcatgaa gatagtcaag cgcgatctgg
     3661 tttttcttcg gcgtgaccat ggcttgccgg atagaggtga atgaccctgg aagaaccttt
     3721 agacgggcat ttccttcggc tgctttttcc agcggctgcc tgaccccggc cgccgcgtcg
     3781 aggttttttt caagaaaaag atcaatggcc gccgccgaag tgtcggcccg gacaatttcg
     3841 gcctttttta gctcgcgggt caaaaacaga tcgtatgcgg cgcctttgcc aacggccacg
     3901 cgaaccccct cagcgtcaat gtcatcaata gagcgtaatg gactgtttcg gttgacgagg
     3961 taagttcctt cgatcagtac atatggcgcg gtgaataaaa ctttctgtcc ccgaaccgga
     4021 tcaatcgcca aaaagccgat atcccacgta ttattatcag tttcggcaaa cactttgcca
     4081 gcggcgtcaa agccaagaat ttcaagctcg acccccaggc gcctggcaag ctcgcgcgcc
     4141 agcgcgacgg agaccccgcc gggcgctcct gtgacggcat ctttctgcgc aaggacgatg
     4201 ttgccataat ttatcgccgc tcttagcttt cccgcgggca gtaatgctct acgcacatca
     4261 gatagagaag aagaactcat aatgatccta aggtaagtac gggttatggg ttacttatga
     4321 ttgagcagga tacacatgac catcaaacag acgacgcgcg gtgcggtaaa aatagccgcg
     4381 ccgggcgttc cattgctgcc cctcaaaatc aacttcggcg cccaccgtca gtatgcccga
     4441 gggcatgcca attcgcagcg ttccgtccgt ggcggcgcga gccgccttgc ttactaccgt
     4501 gcccttgatt tccgcggcaa cagccagaca taaagaagcc gtcaattgca acgccatatg
     4561 cggctggcca ctcgatatca tccgggccgt caaatcaatg tcttcggcgc gaatcgttgc
     4621 gcccgataaa gtcgttgcgg tctggggggg cgacacgaaa ccaacaaacg gcacaacccg
     4681 cttcgcgcgg gcctgctcca ggtcgggcgt aattcccatc gccactgagg catggatgcg
     4741 aatatcgttc aatttcttga gcacgtcagg gcgctcattc aaaatggccg gcaactcggt
     4801 tccggccaac cccaaatcct gagcgttcac gaatacacac gcattggctg catcaaccat
     4861 ggagacgtta atttttccca ggccaggcac atccagcaac tgggcctcat gccctgtcgg
     4921 cagaagagtg cccgtagtgg ctccgcccgg atcaaggaat tccaatcgaa cttttgcgcc
     4981 tttgcccgaa accccgggga tttccaggtc gccgcccacg caagcgcggc cgttttttac
     5041 cggaaacacg gcgtggatca ccttactggt attcgtattg tggatacgca ccaacacgtc
     5101 tccatcggtc gctttcacca acttttcgtc gattgcaaac ggacctatcg ccgaggacat
     5161 attgccgcaa ttaccgctgt aatcaacccg cgcttcgttc accatgactt gcgcaaacgt
     5221 gtagtcgaca tcagcgtcag gccgcgtcga gggtccaacg atgcagacct tggacaggga
     5281 ggagatccct cctcccatcc cgtttaactg gcgtccatga atgtcggggc tgcccatcaa
     5341 cgccaaaaaa agggaatccc actgttctcg gcgcgacggc aaatcgcgcg aatgcaggat
     5401 cagacctttg ctggttccgc cgcgcatgaa tacggcgcgc aggcgagata tgtcatttgc
     5461 ttctgtcatt gcagcttccc cttttacaat ccgcgttttg cgagtgaatc agttcgaacc
     5521 ctacaccgaa cccatctgac gtttttcaag cgcatacttt aacaaagcgg ccgcgcgata
     5581 tacgccgtgc gcaaacttgc cgtagggcat tgtcacgaac atggccatca cgaaaccgag
     5641 atggatcgcc agcaacaacg ccataccgcc tgtgtcgcgc aaaatgagca gcgccaaacc
     5701 agtcaggcca gtcaaaaaca acaatgcaat aaagccccga tccatgggct tttgatttgc
     5761 gtcgccgtgc tctggattac gtcttacatt catccagagc aatcctaccg gtccgataaa
     5821 caggccaatt cctccaataa taccgaatat gacgggcacg cttatcactg gataaggcgc
     5881 gtaccaggca aaaaaatagt gataaatcgt cgcgatggat gtcgccgcaa aacaaaatat
     5941 aaagccatag aatgtcaggt gatgaaagcg ccggcgccat aacgtaaaac gatcatcctc
     6001 gttattacaa ccctcgccgt gaccgccccc cagataacgc aatgtcagca catcttttgt
     6061 cgcctctgtg gcagacgcta ttgcctgtgt tcggttcttg agcgactcgg gcggcgatat
     6121 gttgcgccag aatcgaaccc cgccaatcgc cagcgccaaa accgcaaacg caaacaccga
     6181 cccaaacatc aagaccagtg tgttatgggg aaaaatggca taaaaattgc ccgctaaagg
     6241 agcatgccac aacgaaccat tgataataag aaccagaatt aagaacaagg ccaatccggc
     6301 gcctacagct agtgccgtgg ttagcccggc gcttttgtaa agcgcgccaa aacgggctgg
     6361 ccatgcatat tcgacatagg tctgcatccg cacacgggcc atggctcgtg ggacgttgac
     6421 agcgaattcg tgaggctcgg catattgaca agaatgcaaa catgcgccgc agttatgaca
     6481 taaattggcc aaatagttaa tatcggattt aacaaaatcc agacggtgtg tcatcgcggg
     6541 aaaaacagcg caaaaccctt cgcaatagcg gcacgcgttg caaatttgca tgacgcgatc
     6601 aacttctttt tcgtcatcgg tgagcatcag tgcttcgatc ccggcggtat gagccttctg
     6661 gatcgagacc ggtaatgtct ccatcatcta tccttgattg ttacgcagta atttccgcgc
     6721 cggacttcgc cgccaatgca gcccgtgttc cggctatccg tccaaaggcc gtgcctatcg
     6781 acatgcccac tcccgccgtg tacccctgcc ccagcacatt tccggccatc atttcgccgg
     6841 ctgcaaacaa attggggctg ggttcgccat taaagtgcac tgacgcgttc tcgttgactt
     6901 tgactcccag atacgtaaag gtaatgccgg gcctgagagc atagccaaaa aacggaggcg
     6961 tatcgatagg cattgcccag tgtgttttga caggttcaat accttcagta tggcaatcat
     7021 ccagaaccgc atgattgaac gttccgacat gacaggcggc gttgtatgcg tcgatagttc
     7081 gaacaaaagc agtcggatcc agttcaagct gctgcgccag ttcgcttaac gtattggcct
     7141 tcgcgccggg aaacaccggc ggcatgaatc gccctacggc tttggaatcg atgatcgaat
     7201 aggcgatctg ccccggttgc cgggccacca gcctgcccca tatcgcgtaa cgcttgggcc
     7261 agaaatcctc gccctcgtca taaaatcgcg tggcctgacg attgaccacc actcccagcg
     7321 atacgcaatc gatacgcgtg cagatgccgc catcgtatag cggtgcgcgc gcatcaatgg
     7381 ccacgcaatg ggattggctg ggttcgccta tggagtcggc gccggcgtca agcaaaaatt
     7441 tcaacaaaac gcccatgttg tatcgtgtgc cgcgaatcag gaaattatca gccacccact
     7501 cgccattgat ctggccccac gcttcacgca accacttgcg gttcgattca aagccgccgc
     7561 aagccagcac gcaggccttt gcctggattc gctcgtcgcc aagccgagcc gcgataaaac
     7621 ggccatcgtc cagttcaaga gaatcgacag gcgagttata gcggatatca acgcccagat
     7681 tctgggcgct tcgatagtag gcgttgacca gagccttgcc gcctcccata aagaaggcat
     7741 tggtgcgcgc cacatgtagc gcgcctgtga ggggcggttg gaaatgaacc ccatgctgcc
     7801 tcatccagcc gcggcatgaa gaggatgcgc ggattacgag ccgcgcaagg tgctcgttgg
     7861 tgcgcccgcc agttacttta agcaggtctt gccaaaattc ttcttcgggg taggaaccgc
     7921 tgagaacgtc ctgcggctca tcgtgcatac agcgcaaatt gcgtgtatgt tgggaattgc
     7981 cgccacgcca ctctttcgga gacgattcca gcattaaaac cgaagcgccc gcttcgcgcg
     8041 ccataagtgc cgcgcataaa gccgcgttgc ctccaccaat cactaaaaca tcgatcatga
     8101 aatgctgccc gccaagactt cagcgtaggc tttcgtgcct aacatgccgc ccaaatccag
     8161 ggttcgcgtc gcgcgattgg ccagcgcgtc gtccaccgct ttatcgata
//
LOCUS       DATCXU010000261         6458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1015229,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000261 DATCXU010000000
VERSION     DATCXU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015229"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VIR04_05860"
     CDS             complement(<1..698)
                     /locus_tag="VIR04_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460307.1"
                     /translation="MSEYILETKNLNKEFHGFVAVSDVNLQVERGSIHALIGPNGAGK
                     TTCFNLLTKFLTPTSGRILFSGTEITHEKPAQIARRGIIRSFQISAVFPQLTLLENVR
                     IGLQRPTGLSLHFWRSDKCLDRLNDTAMDLLDQVDLADFANEYTVNLPYGRKRALEIA
                     TTLAMEPELMLLDEPTQGMGHEDVDRVTQLIKKVSAGRTVLMVEHNMKVIASIADKIT
                     VLARGSVLAEGNYQ"
     gene            complement(830..1468)
                     /gene="nth"
                     /locus_tag="VIR04_05865"
     CDS             complement(830..1468)
                     /gene="nth"
                     /locus_tag="VIR04_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY9460308.1"
                     /translation="MNHAKRVEIFARLRAANPAPTTELKYAGAFQLLIAVMLSAQATD
                     KSVNLATAKFFPEHGTPEGLIGLGQEALAGFIKTIGLYRTKAKNIIKTCEILLEQYHG
                     VVPDDRQALQALPGVGRKTANVILNTAFGHAVIAVDTHIFRVANRTGLARGKTVIEVE
                     RKLEKAIPSDYLLNAHHWLILHGRYICVARKPKCPQCAIADLCEYKHKTRLP"
     gene            complement(1465..2100)
                     /gene="rsxB"
                     /locus_tag="VIR04_05870"
     CDS             complement(1465..2100)
                     /gene="rsxB"
                     /locus_tag="VIR04_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEY9460309.1"
                     /translation="MSTLSLTEQIDAALPQTQCTKCGYEGCRPYAEAIANKNEVINRC
                     PPGGQEGVQKLARLLGRPEPTLDPERGPHTPLMVAVIDESHCIGCTLCIQACPVDAIV
                     GASKLMHTVISDLCTGCDLCVAPCPVDCITMVPAGHDWTPSHAHAARIHYQRRQARLG
                     MQHPESSALAKKTLSNKASITPAAHSDANTKRQIIANALARARARRQKATS"
     gene            complement(2156..3382)
                     /gene="phaZ"
                     /locus_tag="VIR04_05875"
     CDS             complement(2156..3382)
                     /gene="phaZ"
                     /locus_tag="VIR04_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY9460310.1"
                     /translation="MLYDLHELQRSFLNPLAAFADTGSRLFSHPYSPFAYLPLSRQIA
                     ASYELVHRIDKDYEKPAWNIDATEIHGERVPVRDRIAVEKPFCNLVHFERQRPESVSA
                     DPTVLLVAPLSGHHATLLRDTVRGLLPAHNIYVTDWVDARMVAASKGPFGLDDYVQYI
                     QDFLQWLGPDTHVISVCQPTVPVLAAISLMASNHHPCMPRSMVMMGGPIDPRKSPTQV
                     NRLATTRPYSWFENNVIHRVPARYPGAGRPVYPGFLQHAGFMAMNPDRHLRSHYDFYL
                     HLISGDDDDAQTHRRFYDEYNAVLDMPAEFYLDTIKIIFQEYRLPLGTWEIAGQKVDP
                     GAISNVSLLTIEGELDDISGQGQTRAAQSLCRSIDKAHKQHYTAPKCGHFGIFSGRRW
                     RETICPKITEFIRQSA"
     gene            complement(3622..3945)
                     /gene="fdxA"
                     /locus_tag="VIR04_05880"
     CDS             complement(3622..3945)
                     /gene="fdxA"
                     /locus_tag="VIR04_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY9460311.1"
                     /translation="MTHVVTENCIKCKFTDCVDVCPVDCFREGPNFLVIDPNECIDCA
                     VCVAECPANAIFAEEDVPADQIDFIALNAELSPSFGEISRSIDPPPDADQWNGVKDKL
                     KYLKR"
     gene            4129..5304
                     /gene="pncB"
                     /locus_tag="VIR04_05885"
     CDS             4129..5304
                     /gene="pncB"
                     /locus_tag="VIR04_05885"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY9460312.1"
                     /translation="MIITSLLDTDLYKFSMMQVVLHHFPAAQVQYRYKNRTPNINLVP
                     YLDEIRAEIHELCQLRFSDDELAYLRSLRFIKSDFADFLGLFHLPERCITVSEGKLPG
                     EIDIRVEGPWLHTILFEIPVLAIVNEVYFRNECREPAWEEGRKRLQSKMHLVIDDPAL
                     ADFRVAEYGTRRRFSKKWHEEVVATMKNQMSPHFAGTSNVLLAMKYGVTPLGTMGHEY
                     LQACQALGPRLRDSQVYALEVWAKEYRGDLGIALSDVYGMDAFLRDFDMYFCKLFDGA
                     RHDSGDPFKWGERLLAHYSQNRADPRTKTLVFSDGLTIPRAIELAARFAGRCKVSFGI
                     GTNLTNDLGHEPLQIVMKMVRCNGQPVAKVSDAPEKTMCDDKAYLAYLRQVFQLPPA"
     gene            5366..>6458
                     /locus_tag="VIR04_05890"
     CDS             5366..>6458
                     /locus_tag="VIR04_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY9460313.1"
                     /translation="MSTIVPPDGALPPFDKTWFERAGEGLDDAGRLLLVQAVDWVRTS
                     LANLTASTGEPLDAHSAAVVRILAELGTDAVTRASALIAVMPVDTANASSNARQDPLR
                     KAFGDEVLLLVQGTRSLLRLGRLAGRSSDSSASGGDQKEMQRKMLLAMAADLRIVLMR
                     LASRLQSLRWYASSKTPCPRQFAQETTELYTPLANRLGIWQIKWEMEDLAFRFLEPES
                     YKSIARQLEEKRAERQAFITVVVERLRAALERAHIVADVSGRPKHIYSIRNKMRTKHI
                     EFNQLFDLHALRVIVDDERACYAVLALVHSLWTPVSNEFDDYISRPKPNGYRSLHTVV
                     SDSEGRTFEIQIRTHEMHRFAEYGMAAHWR"
BASE COUNT         1358 a         1669 c         1896 g         1535 t
ORIGIN      
        1 tgttggtaat tgccttccgc cagcacggag ccgcgcgcca gtacggtaat tttatcggcg
       61 atggacgcaa tcactttcat attgtgctcg accatcagaa cggtgcggcc tgcgctgact
      121 ttcttgataa gctgagttac gcggtcgacg tcttcgtgcc ccataccctg tgtgggctcg
      181 tcaagcagca tgagctcggg ttccatggcc aaagtggtgg cgatttccag agcccgcttg
      241 cgtccgtaag gaagattgac cgtatattcg ttggcaaaat cggccagatc aacctggtcg
      301 agcaggtcca tggcggtatc gttcaatcgg tcaaggcatt tgtcgctgcg ccagaagtgg
      361 agagacaggc ccgttggccg ctgcaatcca atgcgcacat tttccagcag cgtaagttgc
      421 gggaaaacgg ccgaaatctg aaacgaacgg atgattccgc ggcgcgcgat ctgggccggt
      481 ttttcgtgcg tgatttcggt gccggaaaaa agaattcttc cggaggttgg cgtaaggaac
      541 ttggttagca gattgaagca cgtggtcttg ccggcgccat tgggtccgat gagcgcgtga
      601 atgctgcccc gttcgacctg aaggttgacg tcgctgactg caacaaagcc atgaaactct
      661 ttgtttaagt ttttggtttc cagaatgtat tcactcatgc ccgctggtcg tgttcaaatt
      721 ttttagctgt tagtcgtaaa gtctgcatgt tggctgaagt atccgccttt atagctgcat
      781 gcgccatgcg ggtttgtcat aggtcacagg tggcaagcgc gtcatggaat catggtaagc
      841 gggttttgtg tttgtattcg cacaagtcgg cgatggcgca ctgggggcat tttggtttgc
      901 gcgccacgca aatatagcgg ccatgcaaaa tgagccagtg gtgggcgttc aacaggtagt
      961 ctgaggggat ggctttttca agcttccttt ccacttcaat cactgttttc cctcgtgcca
     1021 ggccggttcg attggctacg cggaatatat gcgtgtcgac cgcaatcacc gcgtggccga
     1081 atgctgtatt taaaataacg ttggctgttt tacgcccgac gcccggaagg gcctgcaatg
     1141 cctggcggtc gtcaggcacc acgccgtgat actgctcaag cagaatctcg caggttttta
     1201 tgatgttttt tgccttcgtt ctgtacaagc cgattgtttt tatgaaaccg gccagcgcct
     1261 cctgacccaa gccgatcaac ccttcaggcg tgccgtgttc gggaaagaat tttgccgtgg
     1321 ccaggttgac ggatttgtcg gttgcttgtg ccgaaagcat gaccgctata agcagttgaa
     1381 atgcccctgc atacttgagt tccgtggtgg gcgccgggtt tgccgcccgc aggcgggcaa
     1441 aaatctcgac tcgtttggcg tggttcatga tgtcgccttc tggcggcgcg cccgcgcccg
     1501 cgccagggca ttggcgataa tttgcctttt ggtgttcgcg tcgctgtgcg cggcaggggt
     1561 gatgcttgct ttgtttgaca gcgttttttt ggccagcgca gagctttccg gatgttgcat
     1621 gccaagcctg gcttgccgcc gctgataatg gatgcgcgcg gcatgcgcat ggctgggcgt
     1681 ccagtcgtgg ccggccggga ccatggtgat gcaatcaacg gggcagggcg cgacgcataa
     1741 atcgcagccc gtgcacagat cgctgatgac ggtgtgcatc agtttgctgg cgcctacaat
     1801 tgcatcaacc ggacaggctt gtatgcacag cgtgcaaccg atgcagtgcg actcgtcaat
     1861 cacggcaacc attaaaggtg tgtgcggacc acgttcggga tccagcgtcg gttcgggccg
     1921 gccaagtaat cgggccagct tttgcacgcc ttcctgccct ccgggtgggc agcgattaat
     1981 gacctcgttt ttatttgcta tggcttcggc atagggccgg cagccttcgt aaccgcattt
     2041 ggtgcactgc gtttgcggca gagccgcgtc aatctgctcg gtaagtgata atgtcgacat
     2101 aaaataaaaa tcaggaccca agcgagtcct gatcaggatg aaccgccccg gaacgttacg
     2161 ccgattggcg aatgaactcg gttattttag gacaaattgt ttcgcgccag cggcgcccgg
     2221 agaaaatccc gaaatggccg cacttgggcg ccgtgtaatg ctgcttgtgc gccttatcaa
     2281 tactgcggca caggctttgc gcagcgcgcg tttgcccctg gcctgatatg tcgtccagct
     2341 cgccttcgat ggtcaatagc gagacattgg aaatagcgcc tgggtccact ttttggccgg
     2401 cgatttccca tgtacccaat ggaagccggt attcctgaaa tatgattttg atggtatcca
     2461 gatagaactc ggccggcatg tcgagcacag cgttgtattc gtcgtagaag cgcctgtgcg
     2521 tttgcgcgtc gtcgtcatcg ccgctgatca ggtgcaggta aaaatcataa tgcgaccgaa
     2581 gatgccggtc aggattcatg gccatgaatc cggcatgttg caggaagccc gggtaaaccg
     2641 gccggccggc gcccggataa cgcgcgggaa cgcgatggat gacgttattc tcgaaccagg
     2701 aatacggccg cgtggtggcc agacggttaa cctgcgtggg cgacttgcgt ggatcgatgg
     2761 ggccgcccat catgaccatg ctgcgcggca tgcagggatg atggttgctg gccataagcg
     2821 agatggcggc caataccggc acggtgggct ggcatactga aatgacatgc gtgtccgggc
     2881 cgagccactg aaggaaatct tgaatgtact ggacgtaatc gtccaggcca aacggccctt
     2941 tcgatgccgc gaccatgcgc gcatcgaccc agtcggtgac ataaatattg tgcgcgggca
     3001 acagtccgcg cactgtgtcg cgcagcaaag tggcatgatg ccccgacaaa ggcgcaacca
     3061 gcaagactgt cgggtcggct gagactgatt cggggcgctg gcgctcgaag tgcaccaggt
     3121 tgcaaaatgg cttttcaacc gctatgcgat cgcgcacggg aacgcgttcg ccgtggattt
     3181 cggtagcatc gatgttccag gcgggtttct cgtagtcttt gtctatgcga tggacgagtt
     3241 cgtaagaagc cgcaatctga cgcgaaagcg gcaggtacgc aaacgggctg tagggatgag
     3301 agaaaagccg ggatcccgta tccgcgaaag cggctaaggg atttaaaaat gaccgttgaa
     3361 gctcatgcaa gtcatacaac ataaaaaata agcctctttg gtatgaagcg ctagtcagga
     3421 accggccagc acctggcaag taccgcaatg gtttatagat tgttttacag attgttataa
     3481 gcaaagcata aaataatcta aatagccaga ggcccgctag tattgaattc ccctagtctg
     3541 gggctatatc gttggtttat agggcgcggc agcgctttat cgttattttg tgcaccagcc
     3601 tgtgttggcg tcgcgcaaca tttagcgttt tagatacttc agtttgtctt ttacgccatt
     3661 ccactggtcg gcatcgggcg gcgggtcgat cgaccggctg atttcgccaa aacttggcga
     3721 aagctcggca ttgagcgcga tgaaatcaat ttggtcggcc ggaacgtctt cttcggcgaa
     3781 tatggcattg gcggggcact cggcaacgca gacagcgcag tcgatgcact cgttgggatc
     3841 gatgaccaaa aaattgggtc cctcgcgaaa gcaatcgacc ggacaaacgt cgacgcagtc
     3901 ggtgaatttg catttaatac agttctcggt cacgacgtgc gtcataagaa aagctcccgg
     3961 caaaacatgg caacagtctc tattgcctta tgaagcgatt ttactcaata atgcggggca
     4021 accgcttatt gcccagtctg cgggaatagg tatttggcaa ccggaggccg tggccgggaa
     4081 aatgcccgcc tgttgtgcta tggttgataa aacaacctat tcgccccaat gattattact
     4141 tcgcttcttg ataccgacct gtacaagttc agcatgatgc aagtggttct gcaccatttt
     4201 cccgcagctc aggttcagta tcgttataaa aaccgcacgc caaatatcaa cctcgtgccg
     4261 tacctggacg agattcgtgc cgaaattcat gaactttgtc agttgcgttt ttccgatgac
     4321 gaacttgcgt atcttcggag cctgcggttt ataaaaagtg attttgccga tttcctcggg
     4381 ctgtttcacc tccccgagcg gtgcattact gtgtccgaag ggaaactgcc gggcgaaatc
     4441 gacatcaggg ttgaaggacc ctggctgcac acgattcttt tcgagatacc ggtcctggca
     4501 attgtcaacg aagtatattt tcgcaacgaa tgccgcgagc ccgcctggga agaagggcgc
     4561 aagcgcctgc agtccaaaat gcatctggtt attgacgatc ccgcattggc ggatttccga
     4621 gtggcggaat acggtacgcg ccggcgcttt tccaaaaaat ggcacgaaga agtcgtggcc
     4681 accatgaaga atcagatgag ccctcatttc gccggcacga gcaacgtgtt gctggccatg
     4741 aagtacggcg taacgcctct gggcacgatg ggtcacgaat acttgcaggc gtgtcaggcc
     4801 ctggggccgc gcttgcgcga ttcgcaggtt tatgcgctgg aagtctgggc caaggaatat
     4861 cgtggcgacc tgggaatcgc gttgtccgat gtatatggca tggatgcgtt tttgcgtgat
     4921 ttcgacatgt atttttgcaa actctttgat ggtgcgcggc atgattccgg cgaccccttc
     4981 aaatggggtg aacgcctgct cgcgcattat tcccagaatc gcgccgatcc ccgcaccaag
     5041 accctggttt tctccgacgg attgactatc ccccgggcga tcgaactggc cgcgcgtttt
     5101 gcgggacgtt gcaaagtatc gtttgggata ggcaccaatc tgaccaacga tttggggcat
     5161 gagcccttgc aaatcgtgat gaaaatggtt cgctgcaatg ggcagccagt ggccaaggtg
     5221 tcggatgcgc ctgaaaagac gatgtgcgac gataaggctt atctggcgta cttgcggcag
     5281 gtttttcaac tgccgccggc ttgatcggat aacgatgggc cgcctatttt tacattgacc
     5341 gggttctact gcgccgccac tgaccatgtc tacgatcgtt ccgcctgatg gcgctttgcc
     5401 gcctttcgac aagacatggt tcgagcgcgc gggcgagggc ctggacgatg ctggccgcct
     5461 tttgcttgtt caggccgtgg attgggttcg cacgtcgttg gcgaatctga cggcaagcac
     5521 cggggaaccg ctggacgcgc atagcgccgc ggtcgtgaga attctggcag agctgggcac
     5581 ggatgccgtt acgcgcgcca gtgcgctgat tgcggtcatg ccggtggata cggcgaacgc
     5641 ttcgtccaac gccagacaag accccttgcg caaggctttc ggcgacgaag tgttgttgct
     5701 ggtgcaggga actcgctctc ttttgcgttt gggccgcctg gccgggcgtt ccagcgatag
     5761 ttccgcatcg ggcggcgacc aaaaagaaat gcagcgcaag atgttgcttg ccatggcggc
     5821 cgatctgcgc attgtattaa tgcgtctggc ttcccgtctt cagtccctgc ggtggtacgc
     5881 cagttcgaaa acgccctgtc cgcgtcagtt cgcgcaagaa accactgaac tctacacgcc
     5941 attggccaat cgcttgggaa tatggcagat caaatgggaa atggaggacc tcgcgtttcg
     6001 attcctggaa ccggagagct acaaatccat tgcgcgccag cttgaagaaa agcgcgccga
     6061 gcgacaagcc ttcatcactg ttgtggtcga gcgcttgcgg gccgcgctgg agcgcgcgca
     6121 tattgtggcc gatgtgtctg gacggcccaa acatatttac agcatccgga acaagatgcg
     6181 gacaaaacat atcgagttca accagctctt tgatttgcat gcgctgcgtg tcattgtcga
     6241 tgatgagcga gcctgttatg ccgtgcttgc gttggtgcat tcgttatgga cgcccgtgtc
     6301 caacgagttc gacgattata tttcccgccc gaagccgaat gggtatcggt ccttgcacac
     6361 ggtggtgtcc gattccgaag gccggacatt cgagattcaa atccgcacac acgaaatgca
     6421 ccggtttgcc gaatatggca tggcggctca ttggcgct
//
LOCUS       DATCXU010000262         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_738886,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000262 DATCXU010000000
VERSION     DATCXU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738886"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VIR04_05895"
     CDS             <1..293
                     /locus_tag="VIR04_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY9460314.1"
                     /translation="MVLAGAGGCAAYDVVLILKRGRHAVKRCQVKLSGERATTDPKVF
                     TKIHFAFTVTGADLPISAVERAVQLSHEKYCSATAMLSRTAEITQSTTVIEG"
     gene            300..1094
                     /locus_tag="VIR04_05900"
     CDS             300..1094
                     /locus_tag="VIR04_05900"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY9460315.1"
                     /translation="MHQYEDFMRHVYEHGQAKTDRTGTGTRSVFGYQMRFNLAEGFPL
                     VTTKKLHTKSIFIELLWFLRGDSNVRWLQEQGVTIWDEWATAEGDLGPIYGVQWRAWP
                     TPDGGHIDQITQIIEQIRKNPDSRRMIVSAWNVGDIPNMALPPCHAMFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYALLTHMVAQQCDLGVGDFVWTGGDCHLYSNHLEQVKL
                     QLSRQPYPYPRLRITRKPDSIFDYRYEDFGIIGYESHPHIKAPVAI"
     gene            1106..1600
                     /locus_tag="VIR04_05905"
     CDS             1106..1600
                     /locus_tag="VIR04_05905"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY9460316.1"
                     /translation="MAPEIRLVVAYSDNRVIGRDNALPWRLPADLAHFKRTTLGHPII
                     MGRATWESLGRPLPGRPNIVISRNHQFKADGAAVYPSLEAALAACKTEPVVCIIGGAQ
                     VFEHALGLADQIVATEVHSIIKGDVFFPALDAGQWREIQRLPQPAQNGHDFDFVVYQR
                     RRAA"
     gene            complement(1581..2613)
                     /locus_tag="VIR04_05910"
                     /pseudo
     CDS             complement(1581..2613)
                     /locus_tag="VIR04_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
BASE COUNT          614 a          780 c          673 g          546 t
ORIGIN      
        1 aaatggtgct cgccggcgcc ggcggctgcg ccgcctacga cgtcgttctt atcctcaaaa
       61 gaggacgcca cgcggtcaag cggtgtcagg tcaaactaag tggcgaacgc gcaacaaccg
      121 acccgaaagt cttcacgaaa atccatttcg cttttacggt tactggcgcc gatctgccga
      181 tttcagccgt cgaacgcgct gtgcagctct cgcacgaaaa atattgttcg gcaaccgcca
      241 tgctatccag gacggccgaa atcacgcagt cgaccacggt catagagggt tgagaaccta
      301 tgcaccagta tgaagatttc atgcgccatg tttatgagca cggccaagct aaaacggatc
      361 gcacaggaac tggcacgcgt tccgttttcg gctaccagat gcgtttcaat ctggcggaag
      421 gcttccctct tgtaaccacc aaaaaacttc acacgaaaag tatcttcatc gaactgttgt
      481 ggttcttgcg cggcgattca aacgtgcgct ggctgcagga acaaggggtt acgatctggg
      541 acgagtgggc caccgctgag ggcgaccttg ggcccattta cggcgttcaa tggcgcgcgt
      601 ggccaacccc cgacggtggg catatcgatc aaattacgca aatcatcgaa caaatacgta
      661 aaaatcccga ttcgcggcgg atgattgttt cggcctggaa tgtgggcgac atacccaata
      721 tggccctgcc tccgtgccat gcaatgttcc agttttacgt ggccaatggc aagctgtcgt
      781 gtcagcttta tcaacgtagc gctgatatat tcctgggtgt tcccttcaac atcgccagct
      841 atgcgttgct tacgcacatg gtggctcagc agtgcgatct gggcgtaggc gatttcgtct
      901 ggacgggcgg cgattgccac ctctacagca accatctgga acaggtaaaa ctgcaactat
      961 cgcgccagcc ctacccctat ccgcgcttga gaatcacgcg caaacccgat tcgatttttg
     1021 attatcggta tgaagacttt ggaattatcg gctatgaatc ccatcctcat attaaagcgc
     1081 ccgtcgcaat ttaactgaaa gacccatggc tcctgaaatc agactcgtcg tcgcctattc
     1141 ggacaatcgc gtcattggcc gcgacaacgc cctgccctgg cgactgcccg ccgaccttgc
     1201 acactttaag cgcaccaccc ttggacaccc catcatcatg gggcgcgcca cgtgggaatc
     1261 gttaggccgg ccactgcccg ggcggccgaa tatcgtcatc agccgaaacc atcaattcaa
     1321 agccgacggc gccgctgtct atccatcgct ggaagcagcg ctcgctgcct gcaaaaccga
     1381 gcctgttgtg tgcattattg gcggggctca ggtgtttgaa catgcattgg gcctggcaga
     1441 tcagatcgtt gcgaccgagg tgcattcaat aatcaagggc gacgtttttt ttcccgccct
     1501 cgacgcgggc caatggcggg aaatccagcg tctgcctcag cctgcacaga atggccatga
     1561 ttttgatttt gtggtgtacc agcgccgacg cgccgcatga accgccaaag tagcgggtca
     1621 agcgaataag ccacattgaa tcgaatccag ccgcgatcct cttggtcagg gtcaaagtat
     1681 gagccgggcg ccagccagat tccatctttc aatgccagcc gggccagttc caccgccccg
     1741 cgttcgcgcc acagaccagc gggccgggcc cataaaaaca ggcctgcgcg cggctgaaaa
     1801 aacacttcaa aaccatgctg ctccattaaa tcggccaatt catcatgcgc ttgtctcagg
     1861 cgttcctgca ctctgtgcaa atgcgcacga tgatggccca ggcgcaaaac ttgatacacc
     1921 gcccgctcca tcatttcggg ggtagtcagt cccattacca tttttgtctt ggcgaattcg
     1981 ccgagcaacg gcgcggtaga aacgacataa cccacacgca ccgacggggt aatgcttttc
     2041 gaaaatcccg acacgtacac gacccgtgcc gtgcctgcca aggcgaccag catgggacca
     2101 acgccgggca aaagattgcg cgacacgtcg tcttcaatta cccagaaatc atgcttttcg
     2161 gcaagctgca acacacgaaa ggcattggcc atgctcaaac tggagcctgt tggattgtgc
     2221 agcactgtgt tgataaaaat gcctttcagg tcatgctgca aggcgacctt ttcaagagct
     2281 tcgatgcaca ggccatccct gtcgcgggga actcccacaa cctttaaccc ggccaaactc
     2341 caggcgtgca ataaattggc ataacacggc gcctcgacgg ccaccacgtc gccaggccga
     2401 aacagggttc gcgtcacaat gtccaagcct tgcgtcgctc cctgggtcaa aagcacttga
     2461 tcaagctgca gattcaaacc gagctgacca agggcctgaa cgatgtattc ccgcaaaggc
     2521 gcgtagccgt atgggtgtcc ataaccggca atctgaaccg aagggacccg cgccaattgc
     2581 cgcagcgatt gctgcaaacc tgcttcgtta agc
//
LOCUS       DATCXU010000263         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_708303,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000263 DATCXU010000000
VERSION     DATCXU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708303"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VIR04_05915"
     CDS             complement(<1..1431)
                     /locus_tag="VIR04_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9460317.1"
                     /translation="MIEQSLTDLWYGFGIAFEPHNLMWSIFGVVAGNLMGVLPGLGAM
                     AAMSILLPLTYAMHPVPAILMLAGIFYGSMYGGAIGAILLNLPVHPPHAVACLDGYPL
                     TVKGRGGAALGIAMIASFFAASVGILVMVFFSPLLVDVAFKFGPAELFSIMLLGLLAG
                     STMSKGSAVKGVAVTLFGLLIGVAGTDVNTGTMRLTFGMLGLEDGVDLVALAMGLFGI
                     GEFLLHVNRMKTVGSGLKMRFRDMRPTKSELKRSFFPMLRGTAVGTFFGALPGTGPTI
                     TTFVAYALEGKVSKNPEKFGHGAIEGVASPEAASHSKTQVDFIPTMTLGIPGDEVMAL
                     MLGALLIQGIQPGPQLIISHPDIFWGLIASFWVGNLLLVLLNVPLISVWVKLLQVPYR
                     YLFPSALFFIAVGCYSTNNSLFEVGEVLVFGVVGAVLIYLDFSVATALLGYVLGPMIE
                     ENFRRALLLSRGDIMVFVERPISAWFI"
     gene            complement(1435..1848)
                     /locus_tag="VIR04_05920"
     CDS             complement(1435..1848)
                     /locus_tag="VIR04_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY9460318.1"
                     /translation="MILIGLGAILEGTNYRIGTLSQMGPGFFPAAIGALMVLMGVIIA
                     IEGLGHGHVTTDHDLPPEYRGWACILGGIAAFIVLGEFTGLLPATFAIVFISALGDRQ
                     NTVKNAFILAVAMCFVATGVFWWALQLQFPLLHWG"
     gene            complement(2159..>2499)
                     /locus_tag="VIR04_05925"
     CDS             complement(2159..>2499)
                     /locus_tag="VIR04_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY9460319.1"
                     /translation="QILAGTIKPLAVSTDKPSRFFPTLPTMASSGVPGYNLQTWYGLL
                     LPAGTPERIRDTLYKDTVQALKDPKLVKMFESLGADAGGMKPAEFKTFIVDQTKQWAS
                     VAKAVDMKKN"
BASE COUNT          659 a          745 c          637 g          458 t
ORIGIN      
        1 aatgaaccag gcgctgatcg ggcgctctac aaagaccatg atatcgccac gcgagagcaa
       61 aagcgcacgg cggaagtttt cttcaatcat gggacccagc acatagccta gcaatgccgt
      121 cgccaccgaa aaatccaggt agattagcac tgcgcccacg acgccaaaaa ccagcacctc
      181 acccacttcg aacaagctgt tgttcgtgct gtaacaccca acagcaataa agaacaaagc
      241 agaaggaaac aaatatcgat aaggcacctg caacagcttg acccaaacgc tgatcaatgg
      301 cacgttcaac agcaccagca acagattgcc tacccagaag cttgcgatca ggccccagaa
      361 aatatcagga tgcgaaatga tcagttgtgg tccaggctgg atgccctgaa tcagcagggc
      421 tcccagcatc agtgccatga cctcgtcgcc tggaatgccc agcgtcatgg tgggaataaa
      481 gtccacctgc gtcttggaat gcgatgcggc ctcgggcgaa gccacgccct cgatcgcgcc
      541 gtgcccgaac ttctcgggat tcttggaaac cttgccttcc agcgcgtacg ccacaaacgt
      601 tgtaatagtg gggccggtac cgggcaatgc gccgaaaaag gttccgacgg cggtgccccg
      661 cagcattgga aagaaggaac gcttcagctc gcttttggtc ggacgcatgt cgcgaaaacg
      721 cattttcagg ccgctgccca ctgtcttcat ccggttgacg tgcagcaaaa attcaccgat
      781 cccgaacaag cccatggcca acgccaccag gtcgacaccg tcttcaagtc ccagcatgcc
      841 aaaggtcagg cgcattgtgc cggtgttcac atccgtgcca gcgaccccga tcagcaagcc
      901 gaacaaggtt accgccacgc cttttactgc ggaccccttg gacatggtcg agcccgccaa
      961 cagtcctagc agcatgatcg aaaacagctc cgccgggcca aacttaaagg ccacgtcaac
     1021 caacaatggc gagaagaaca ccatcaccag aatcccaacg gatgcggcaa aaaacgaagc
     1081 gatcatcgca atgcccagcg ctgccccgcc ccgccctttc accgtcaacg gatagccatc
     1141 cagacaggcc accgcatgcg gcggatgcac cggcagattc agtaaaatcg cgccgatagc
     1201 gcccccgtac atggatccgt aaaagattcc ggccagcatc aagatagccg ggacgggatg
     1261 catggcatag gtaagcggca aaagaatgga catggccgcc atggcgccca gccccggcaa
     1321 tacgcccatc aagttgccgg ctaccacgcc aaagatggac cacattaaat tgtgaggctc
     1381 aaacgcaatg ccaaacccat accaaaggtc agtgagtgat tgttctatca tcgcttagcc
     1441 ccaatgcaga agagggaatt gcaattgcag cgcccaccag aaaacaccag tggccacaaa
     1501 gcacatggcc accgccagaa taaatgcatt tttaactgta ttttgtcggt cgccgagcgc
     1561 cgaaataaag acgatggcga acgtcgctgg caataagccg gtgaactcgc ccagcacaat
     1621 aaacgccgcg atcccgccaa gtatgcaagc ccagccgcga tattcaggag gaagatcgtg
     1681 atcggtggtg acatgaccgt gccccaaccc ttcgatcgcg ataatgacgc ccatcaatac
     1741 cattaatgcg ccaatggccg cgggaaaaaa ccccgggccc atttgactta acgtgccgat
     1801 ccggtagttc gttccctcga gtatggctcc cagccctata aggatcatta gggcgccgcc
     1861 gtaataatcc ttgttgatgc tacgcttatg caccgatgtt gctcctctcc gcgcctgcag
     1921 aaaataatag ttaccgagcg aatgacaaat cgccttgcag ggataacgtg ggaatataaa
     1981 gaatgaatct ttacagggag cttaatttca gggtatacac cctgttcacc aaataagaag
     2041 gccacatcag catggctgaa aggcctgtgc ccccaggcgg aagcgccgtg tcatggtggc
     2101 tgcgtcgcct ggaaaatcgc cggtggcaac gcgaccgccg gcgcgggttt taaataaatt
     2161 agtttttctt catgtcgacg gccttggcca ccgaggccca ctgcttggtc tgatcgacga
     2221 taaaggtttt aaactcggcc ggcttcatgc cgcccgcatc ggcgcccaaa gattcgaaca
     2281 ttttgaccag cttcggatct ttcaatgcct gcaccgtatc cttgtagaga gtgtcgcgaa
     2341 tcctctccgg cgtgccggcc ggcaacaaca agccatacca ggtttgcaag ttgtacccag
     2401 gaacgcccga ggacgccatc gttggcaaag tcgggaaaaa acgggacggc ttgtcggtgc
     2461 tgacggctag cggtttgatc gtgccagcca gaatttgcg
//
LOCUS       DATCXU010000264         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_325612,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000264 DATCXU010000000
VERSION     DATCXU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325612"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="glmU"
                     /locus_tag="VIR04_05930"
     CDS             <1..1221
                     /gene="glmU"
                     /locus_tag="VIR04_05930"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY9460320.1"
                     /translation="HGAERVRAAFADQPGLQFVLQSPQQGTGHAVQQAVPLLLGGDND
                     ADTTLVLYGDVPLVQTQTLKRLLDARKNAMALLTENLADPSGYGRILRDAKGRVVRIV
                     EQKDASAQELAINEINTGILAAPTGRLKGWLAQIDNRNAQGEYYLTDVVGLAVRDSVA
                     VNSAQSAAPWETLGVNSRVQQAQLERIWQGEQARRLLEQGVTLADPQRFDLRGTLVCG
                     RDVFIDVGCVFEGRVELADGVRIGPHCVIKDATLGAQTQVEAFTHIQQAVVADEAHIG
                     PYARLRPGADIGPHAHVGNFVEIKKSRLGEGSKANHLTYIGDAQIGARVNIGAGTITC
                     NYDGVNKFPTVIEDDAFIGSDTQLVAPVRVGRGATLGAGTTLVKDAPADQLTISRTPQ
                     KTIQGWVRPTKKPK"
     gene            1221..>2536
                     /locus_tag="VIR04_05935"
     CDS             1221..>2536
                     /locus_tag="VIR04_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9460321.1"
                     /translation="MATRDPGSKKSAMLADGLEGSERHWAVVTLMAALAVTVLDSSVA
                     NVALPTIARDLAIDPSAVVWVVIAYSLTIVVFLLPFSAVAERIGFRRMFALGITVFML
                     ASLGCALSVSLASLIVSRVVQGLGASMLMCLFGGLVRNIYSSRDLGFGISLNAMMVGL
                     MSVLGPSIGAFILGIATWPWIFAINIPVCLLVYFGIRFLPDVPRSTARFDWIACGLSM
                     VFFGLSVIGLDALAREPSRAALCLVAACGAGIVLVRRSRDQTAPLVPIDLLRIKPVAF
                     AVGASAFSFAAQMAAFVALPFYFLKVMGHSYFEVGILLGSWSGGVMLMAPLAAYMSNR
                     YPVAILCAIGALGMICGLSWVIVLPLGASIVWIMMAMLMGGIGFGFFQTPNNRAMLGG
                     APRIRSGAAGGLQATTRVFGQSFGTALVAIAFGSSATYGALLGILVA"
BASE COUNT          442 a          678 c          815 g          601 t
ORIGIN      
        1 catggcgccg agcgcgtacg cgctgcgttt gccgatcaac ccggccttca gttcgtgttg
       61 caaagcccgc agcaggggac tggtcatgcg gtgcaacaag ccgtgccttt attgcttggc
      121 ggcgacaatg acgccgatac cactctggtg ttgtacggcg atgttcccct ggtgcaaact
      181 cagacattga aacgtttgct tgatgcgcgc aaaaacgcca tggcgctgct cacggaaaac
      241 ctggcggacc catctggtta tgggcgcatc ttgcgcgacg cgaagggccg ggttgtgcgt
      301 atcgttgaac agaaggacgc aagcgcgcaa gaattggcca tcaatgaaat taatacagga
      361 attttagcgg cgccaaccgg gcggcttaaa ggctggctgg cgcagattga taatcgcaat
      421 gcgcaaggcg agtattacct gaccgatgtg gtgggcctgg ctgtgcgcga cagtgtggcg
      481 gtcaattccg cacaatcagc ggcgccgtgg gaaacattgg gtgtaaacag ccgcgtgcaa
      541 caagcgcagc tcgagcggat ttggcagggc gaacaggcgc gccgcttact ggagcagggg
      601 gtgacgcttg ccgacccgca acgcttcgac ctgcgtggaa cgcttgtgtg cgggcgcgac
      661 gtgttcattg acgtcggttg tgttttcgaa ggccgcgttg agctggccga tggcgtacgt
      721 attggaccgc attgcgtcat taaggacgca accctggggg cgcaaacaca ggtcgaggca
      781 tttacccata tacaacaggc tgtcgtggcc gatgaggctc atatcggccc atacgcgcgc
      841 ttgcgtccgg gcgcggatat tgggccgcac gcgcatgttg gcaactttgt agagattaaa
      901 aaaagccggt tgggcgaagg atccaaagcc aatcatctga cttatatcgg cgatgcgcaa
      961 attggggcgc gcgtaaatat tggggcgggc acgattacgt gcaattacga cggggtgaac
     1021 aagttcccga cggtgatcga agacgacgcg tttatcggtt cggatactca gcttgtggcg
     1081 cccgtgcggg tcggccgggg cgccacgctg ggcgccggaa caacgcttgt caaagacgct
     1141 ccggcggatc agctcaccat ttcccggacg ccgcaaaaaa cgattcaggg ctgggttcga
     1201 ccaactaaaa aaccgaaata atggcaacgc gcgatccagg ctcaaaaaaa tccgcgatgc
     1261 tggctgacgg cctggaaggt tctgaacgtc attgggcggt tgtcacgcta atggccgctc
     1321 tggctgtgac cgtgctggac tcgtccgtcg ccaatgtcgc cttgcccacg attgcgcgcg
     1381 acctggcgat tgatccgtct gccgtggtgt gggtggtcat tgcctatagc cttaccattg
     1441 ttgtgttctt gctgcctttc tccgcggtgg ccgagcgtat cggctttcgg cgaatgttcg
     1501 ccctgggcat taccgtgttc atgcttgcct cgctgggttg cgccttgtcc gtgtctctgg
     1561 caagtctgat tgtctcccgg gtcgtacagg gtcttggcgc gtccatgctg atgtgcctgt
     1621 ttggcggtct ggtgcgcaat atctattcat cgcgcgacct cggtttcggt attagtctga
     1681 atgcgatgat ggttggacta atgtctgtac tggggccttc catcggggca ttcattttgg
     1741 gcatcgccac ctggccgtgg attttcgcca tcaacatacc ggtctgtctg ctggtctatt
     1801 tcggtattcg cttcctgccg gatgtgcctc gcagcacggc gcgctttgac tggatcgcct
     1861 gcggcctgag catggttttt tttggcttgt cggtaattgg tctggacgcg ctcgccagag
     1921 agccgtcgcg cgccgcatta tgcctggtgg cggcgtgcgg ggcggggatt gtgctggtgc
     1981 gccgctcgcg tgatcagaca gcgccgctgg tgccgattga tttactgcgc atcaagccgg
     2041 ttgctttcgc ggtgggcgcg tcggcttttt cgtttgccgc gcaaatggcg gcgtttgtcg
     2101 ccctgccttt ttacttcctg aaagtgatgg gccacagtta tttcgaagtc ggcatattgc
     2161 tgggatcgtg gtcggggggc gtgatgctga tggctcccct tgcggcttat atgtccaaca
     2221 gatacccggt cgccattctt tgcgccattg gcgcgcttgg catgatttgc ggcctgtcgt
     2281 gggttattgt gctgcctttg ggggccagca tagtgtggat catgatggcg atgctgatgg
     2341 gaggcattgg cttcggcttt tttcagacac ccaataatcg cgccatgctg ggcggcgctc
     2401 ccagaatacg cagcggcgcg gcgggcggcc tgcaggcaac aacccgtgtg ttcggtcaaa
     2461 gtttcggcac ggcgctggtg gccatcgcat tcggttccag cgcgacttac ggcgccttgc
     2521 taggcatact ggtggc
//
LOCUS       DATCXU010000265         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_197948,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000265 DATCXU010000000
VERSION     DATCXU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197948"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VIR04_05940"
     CDS             complement(<1..361)
                     /locus_tag="VIR04_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460322.1"
                     /translation="MHTKASFKRVFKVCAIAGLLGVGGAAYAADEMPIGVVTPLTGGG
                     AQFGPDMAKAMQLAADEVNKVGPPMGRKFRLYVEDGETNPDAGVRAAQKLIGVNRVKA
                     ILGTWSSAVTLAVAPLTI"
     gene            complement(373..828)
                     /locus_tag="VIR04_05945"
     CDS             complement(373..828)
                     /locus_tag="VIR04_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460323.1"
                     /translation="MEQYDVVRQIARFVSIPGIEPVQTHFGETYANSTEWWAVKLREA
                     DSTFAWLAYSFGDIPMWALHAGVVLTETGARVGLHCHKKAEPEYCEAVKNIGQHIGPL
                     YDSLSADELQYNQDFSGFGPDVLQEVGIRLGQVYVDARSGLIERGLINK"
     gene            complement(873..2114)
                     /locus_tag="VIR04_05950"
     CDS             complement(873..2114)
                     /locus_tag="VIR04_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460324.1"
                     /translation="MISDKSKEILIGLETTIGEKWALHAIRGAAADEFLHVIIADGMF
                     STKVDGFSHAAHVTTPYMLSYLEEPDTALNLTALTEWEKASNSAIKFAHIQAEFHWKI
                     DTRLWQYFIYTKYMRRAYDDAIVESEMDDGDKEYLRAALSRFWDEVEIAYTYRFEAIE
                     RSRGFAPHPDPTAGSSDHGYVYRKLLETPDYKFEEFKFPMIGANFGAGTAKDIRVPPA
                     WPSLPQIQREGLLAHAWTWYSEARDAGLADTFLRARLGARMVPTRNNGWEKGLVESNQ
                     FLAGDSDLLPGNPSIEERAVLFARTQFAFALRLLANMSEYLALFKTQKKALEAWHAEE
                     GSGAQIAKFFEEVEKEFASLARDDRKEADFVDDKVKAIIEDSRPIHPRYADFDPIEPL
                     PWQTRNPERYLTRMKKLGYTL"
     gene            2638..>3544
                     /locus_tag="VIR04_05955"
     CDS             2638..>3544
                     /locus_tag="VIR04_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460325.1"
                     /translation="MLKTCLFTALTFCAVMFSPGAAAICTINSVTVSCSNLQITVPNQ
                     TVLIETTSTASGVIGLAASASSGINVTNHGTISGIFLGVLNGGTIDSFVNYGSVTGRL
                     SNGFGNSGSMTVLSNFGSIANSLNNQGTITTLNNLQGGATPLVILTDAGSGSIPVRLP
                     QNYNVIINSSSNYGKFSVTGPPSTDMMNFGIYSDSIVAATTYSSVLDGILASNLASTS
                     GVFGAFNWSLVNSSGTIWDLIFTPAFIPPGPHMGPGQSFTLDDVGVGVNPVFNGGTLT
                     LAVGNNSNQAFTVQGAGGTITAPTDG"
BASE COUNT          797 a         1000 c          854 g          893 t
ORIGIN      
        1 ggatagtcaa tggcgcaaca gcgagcgtga ccgcgcttga ccatgtgccc aatattgcct
       61 tgacccggtt cacgccaata agtttttgcg cagctctgac gcccgcatca ggattcgtct
      121 cgccatcttc gacgtacaga cgaaattttc ttcccatggg gggaccgacc ttgttgactt
      181 catccgcggc cagttgcatg gctttcgcca tatcgggacc aaattgggct cctccgccgg
      241 tcaaaggcgt aacgacacca ataggcatct cgtcggccgc atatgcagca ccgccaaccc
      301 ccaacagtcc cgcaatggcg cagaccttga atactcgctt aaagcttgct ttcgtatgca
      361 tataatgtct cctcacttgt ttatcaatcc acgctctatc aaaccactcc gtgcgtcgac
      421 atatacttgt cccagtctta tgccgacttc ctgcaataca tctggcccaa accctgaaaa
      481 atcctgattg tattgaagct cgtcagccga caagctgtca tacagaggcc caatatgttg
      541 ccctatattt tttacggcct cgcaatattc tggttcggct ttcttatggc aatgcaagcc
      601 aacccgcgcg cccgtttccg ttagcacgac cccggcatgg agtgcccaca taggaatgtc
      661 gccaaaagag tatgcgagcc aggcgaaggt cgagtcggcc tcacgcagct tgactgccca
      721 ccactcggtc gaattcgcat aggtttctcc aaagtgcgtc tgaaccggtt cgatgccggg
      781 aatacttaca aatcgggcaa tttgacgcac cacgtcgtat tgttccatag ccaccccttt
      841 tactattatt caggtctctg gaagacgctt tgttataacg tgtacccgag ctttttcatg
      901 cgcgtaaggt aacgctccgg attgcgcgtc tgccagggca acggctcgat cggatcgaaa
      961 tcggcataac gcggatggat gggccttgaa tcttctatga tggccttgac tttatcatcc
     1021 acaaaatcgg cctcctttcg atcatcgcgt gcaagcgaag caaattcttt ttcgacctct
     1081 tcgaaaaatt tcgcgatctg cgctccagat ccttcctcgg catgccatgc ttccaacgcc
     1141 tttttctgtg ttttgaaaag tgcaaggtac tcggacatat ttgccaacaa gcgcagtgca
     1201 aaggcaaact gggttcgggc gaacaggacg gcccgctcct cgatcgacgg atttccgggt
     1261 agcaaatcgc tatcccctgc caggaactgg ttggattcaa ccaacccctt ttcccaacca
     1321 ttatttcgtg tcggcaccat gcgagcgccc aacctggctc gcaagaaagt atcggccaac
     1381 ccagcatcac gcgcttcgga ataccaagtc caggcatgcg ccagaagccc ttcacgctga
     1441 atttgaggta gggaaggcca ggccggcggc acacgaatat ctttggcagt cccggcgcca
     1501 aaattggcgc cgatcatcgg aaatttgaat tcctcgaatt tatagtccgg agtctccagg
     1561 agttttcgat acacgtaacc gtggtcgctg gagcctgccg tcgggtccgg atgcggcgca
     1621 aaacctcgtg acctttctat cgcttcgaac cgatacgtgt acgctatttc gacctcatcc
     1681 caaaaccttg agagtgccgc acgaagatac tccttatccc catcgtccat ttccgattcg
     1741 acgatggcgt cgtcatacgc cctgcgcata tatttggtgt aaatgaaata ctgccacagg
     1801 cgagtgtcga ttttccaatg aaactctgcc tgtatgtgag caaacttgat cgctgaattg
     1861 ctggcctttt cccactctgt gagcgccgtc agattcagcg cagtgtcagg ctcttccagg
     1921 tatgagagca tgtacggagt ggttacgtga gctgcgtgtg agaaaccgtc taccttggtg
     1981 ctaaacattc catccgcgat aatcacgtga aggaactcgt ccgccgctgc cccccgtata
     2041 gcgtgcaagg cccatttttc gccaattgta gtttccagac cgatcaatat ttccttggat
     2101 ttatcgctaa tcatgatcca cttcctaaaa atacaaggtt gcagcgcgca tactgccgcc
     2161 tgactgtcgc acgtgtgtac gcgggttaca ccttttgggt tcggagaaat gtagcacgct
     2221 gacgggatgg gtacaaccta ttttgaatta gtgttagtac ctatgaagaa gcccttcgca
     2281 cggattaaag cgacctcgcg caggctatta cagggccttg gcgttcgcat atatgggttg
     2341 caccttttag gtcaaacgaa atgaattagg tatgcaaaat aggtgcaacc tgtttttggc
     2401 tttgttagca ccggaagtcc cacggcaatc tcgagccctt tggccaggcc gataccgact
     2461 gagcgcggaa ggttgagagg cgctactccc tttgggggct tggccaaagc aataattaac
     2521 actttgttgc gctattgcaa cgattagtga attagataag gtatagtaat actgtaaatc
     2581 cgcagttctg ggcttccagg ttttgcggaa atctgctttt cgcaacaggt tcccgccatg
     2641 ctcaaaactt gccttttcac tgcattaaca ttctgcgccg ttatgttttc gcctggcgcc
     2701 gcagcaattt gcactatcaa cagcgttact gtcagttgca gcaatctgca aatcactgtc
     2761 ccgaatcaaa cggtcttgat tgaaacgacc tccactgcaa gtggggtcat cggacttgct
     2821 gcttcggcta gttcaggaat caatgttaca aaccatggga ccatttctgg catctttttg
     2881 ggtgttctca acggcggaac gatagacagt ttcgttaatt acggttcagt gacggggcgg
     2941 cttagcaatg gctttggtaa tagcggatcc atgacggtgc taagcaattt tggctccata
     3001 gcaaattctc tcaacaacca gggcaccatt accacgctga acaatctcca gggtggggcc
     3061 acgccacttg tcattttgac tgatgccgga tcgggcagta ttcctgtccg gctgccccag
     3121 aattacaacg tcatcatcaa tagctcgtcg aattacggta aattctccgt cacaggcccg
     3181 ccgagtaccg atatgatgaa tttcggcatc tattccgatt ctatcgtggc ggccaccacg
     3241 tattcctcgg tccttgatgg catactcgcc agcaacctgg ccagcaccag cggcgtgttc
     3301 ggggcattta actggtccct ggtcaattcg tcgggcacca tctgggacct gatctttacg
     3361 cctgccttca tccctcccgg cccccacatg ggccccggcc aatcctttac gctggatgat
     3421 gtcggcgtcg gcgtcaaccc cgtctttaat ggcggcaccc tgacgctggc ggtgggcaat
     3481 aactccaacc aggcgttcac cgtgcaaggc gcaggtggta ccatcacggc gcccaccgat
     3541 ggcg
//
LOCUS       DATCXU010000266         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_290484,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000266 DATCXU010000000
VERSION     DATCXU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290484"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VIR04_05960"
     CDS             complement(<1..508)
                     /locus_tag="VIR04_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY9460326.1"
                     /translation="MNAPVLSQSLRRTIPDGFLNTLKERFGERFSGTQAVRDHHGRDE
                     SPYPPMLPDAVVFAQSTDDVAWVARQCNAHKVPLIAFGTGTSLEGHVLAVQGGISLDL
                     SGLNRIVSVNPEDLTVTVQAGVTRKQLNNEIRDTGLFFPIDPGADASLGGMAATRASG
                     TNAVRYGTM"
     gene            complement(558..1652)
                     /locus_tag="VIR04_05965"
     CDS             complement(558..1652)
                     /locus_tag="VIR04_05965"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY9460327.1"
                     /translation="MSHNTDDLRIREIKELSPPAHVMRELVCTPEISANVYGARHAIH
                     NILNGLDDRLAVIIGPCSIHNPVAALDYARHLVAQRDRFKDDLEIIMRVYFEKPRTTV
                     GWKGLINDPTLDGCFNINQGLRTARQVLLDVNRLGLPAGCEFLDMITPQYIADLVSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNVKITVDAINAASHSHHFLSVTKGGRS
                     AIVCTVGNDDCHVILRGGKEPNYDAANVDLACQRLSKAGLRERIMIDASHSNSHKDYR
                     NQPLVVEDVAGQIGGGDARIFGIMVESNLIDGRQDLAVGKPLVYGQSITDGCIGWDSS
                     VKVLERLSEAVKSRRKVIARSEAAASDRAA"
     gene            complement(1858..>2237)
                     /locus_tag="VIR04_05970"
     CDS             complement(1858..>2237)
                     /locus_tag="VIR04_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY9460328.1"
                     /translation="HLPMPRMTNTYMLAGNEPAAGIIASVKKGLYAANFGGGQVDITS
                     GKFVFSASEAYMIENGKVTYPVKGATLIGSGSQAMTQVSLIGDDLQLDSGVGTCGKEG
                     QNVPVGVGMPTLRIEGLTVGGTA"
BASE COUNT          506 a          663 c          586 g          482 t
ORIGIN      
        1 gcatcgtgcc gtagcgcacc gcgttcgtac ccgatgcgcg cgtcgcagcc atgccgccca
       61 aactggcatc ggcgcccgga tcgatcggga aaaacaggcc cgtatcgcgg atttcgttat
      121 tgagctgctt gcgcgtcacg cccgcctgca ccgtaacggt caggtcttcg ggattgaccg
      181 acacaatccg gttcaggccc gataaatcca ggcttattcc gccctggaca gccagcacat
      241 gtccttccag agaagtgcct gttccaaatg caatcagcgg cactttatgc gcgttgcact
      301 gcctggccac ccatgcgacg tcgtcggtgc tttgcgcaaa caccaccgca tcgggcaaca
      361 tcggcggata cggcgattcg tcgcggccat gatggtcgcg caccgcctgc gtgccggaaa
      421 agcgctcgcc aaatcgttct ttaagcgtat tcagaaaccc atccgggata gtgcggcgca
      481 ggctttggga aagaacaggg gcgttcatat tgggtatctc ctttactttt tgaccctgcc
      541 gccgccggca attattgtta tgcagcccga tcgctggccg ccgcctcgga ccgcgcaata
      601 accttgcggc ggcttttcac cgcttccgac aggcgttcca gcaccttgac cgacgagtcc
      661 caccctatgc acccatcggt aatgctttgt ccataaacca aaggcttgcc aacggccaga
      721 tcctgacgtc catcgattaa attgctctct accataatac caaagatgcg cgcatcgcca
      781 cctccgatct ggccagccac atcttcgacc accaaaggct gatttctata atctttatgg
      841 ctgttggaat ggctggcgtc gatcattata cgttcgcgca atcccgcctt cgacaaacgt
      901 tgacacgcca agtcaacatt tgccgcgtcg taattgggct ccttgccgcc acgcaatatc
      961 acatggcagt catcgtttcc gaccgtgcag acaatggcgg aacggccgcc cttggtcacc
     1021 gacagaaaat gatgggaatg agaagcggca ttgatcgcgt cgacggtaat ctttacgttg
     1081 ccgtcggtgc cgttcttgaa cccgacgggg cacgacagac cggaagccag ctcgcgatgc
     1141 acctggcttt cagtcgtgcg ggcgccgatg gccccccagg acaccagatc ggcaatatat
     1201 tgcggagtaa tcatatccag gaactcgcat cccgccggca agcccaggcg gttaacgtca
     1261 aggagcactt gacgcgccgt gcgcaggccc tggttaatat taaagcagcc atccagggta
     1321 ggatcgttaa tcagaccttt ccaacccacc gtcgtgcgcg gtttttcgaa ataaacgcgc
     1381 atgattatct cgagatcgtc tttaaagcga tcgcgttgcg caaccagatg cctggcataa
     1441 tccagtgccg ccacgggatt atgaatggag catggcccta tgattacggc cagacggtcg
     1501 tcaaggccat tcaatatatt gtgtatggcg tggcgcgcgc catacacgtt ggcagagatc
     1561 tcgggcgtgc aaacgagctc gcgcatgaca tgcgcgggcg ggctaagctc tttgatttct
     1621 cgaatacgta aatcgtcggt attgtgtgac ataagctaac tccagagtaa ttgtcaacac
     1681 agtggcgaaa caaaaaagcc gcctagctga gctggcggct tttgggaaat ttcaggttct
     1741 tactgtgtac aacggttccc ttcctccacc agcggcttcg gaaaagtaaa ataaaagaag
     1801 aaaaatcggg ctactacaca catgattctg caatactagc acaaaatcca aagaaaacta
     1861 agctgtgccg ccaacggtca gcccctcgat gcgcaaagtg ggcatcccaa cgccgacagg
     1921 gacgttctgc ccctctttac cgcaagtgcc tacgcccgaa tcgagctgca ggtcgtcgcc
     1981 gatcaggctg acctgggtca ttgcctggga accgctcccg atcagcgtgg cgcctttaac
     2041 gggatacgtt accttgccat tttcaatcat gtaggcctcg gatgccgaaa aaacgaattt
     2101 tccgctagtg atatcgacct ggccgccacc gaagttggcg gcgtacaaac cctttttaac
     2161 cgatgcgata atgcctgccg caggctcgtt ccccgccagc atgtaggtgt tcgtcatgcg
     2221 gggcatggga aggtggg
//
LOCUS       DATCXU010000267         8395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_249403,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000267 DATCXU010000000
VERSION     DATCXU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249403"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1886
                     /locus_tag="VIR04_05975"
     CDS             420..1886
                     /locus_tag="VIR04_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460329.1"
                     /translation="MDPLFHSLTPNIQMSETIFDPLVRTDANAKPIPALAESWTVDGN
                     VWLFKLRPNVKFSDGSPFTADDVVFTYARVPKVPNSPSSFSLFLSSIAKVEAVDSLTL
                     KITTKGPSPVLLANLSQVSIMSKKAAWGSAPEGKTTVELNRGEGLVGTGPYKFVSWKR
                     GDEIVLARNDHYWGKKPAWDKVIYRPISNPAARVAALLAGDVDLVEDPPTDDLPRLTK
                     NKALTVQETPSLRVIYLAMNQSKEVPPGMTGTNGKNALQDKRVREALSLALDRKAIVA
                     RIMGGVAKPAANLLAYPGFGASKKLSEVPASDPVKAKKLLTEAGYPNGFSISLGSPAG
                     RYTNDKNIAQAAAAMWARIGVKTDVQTMAPPVFFKQRDTYAFSTYLGGWAVTSGEMLN
                     PLNALVVTPNKELGLGTTNRSHYSNPELDKVVLQASKTLDDGKRSELLQKAGNMAMED
                     YAILPVHFELSVWAMKKDVHYAGRADQMTLAQDMTPAK"
     gene            1899..2873
                     /locus_tag="VIR04_05980"
     CDS             1899..2873
                     /locus_tag="VIR04_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460330.1"
                     /translation="MFSAIVRRLLQTVVVVLAMSALVFAGLYLVGDPVSMLASPEATD
                     AQRELIRQSLGLNLPLWEQYFIFLGKAAHLDFGNSFLTGESAMRLILDRMPATLELAT
                     LAMFMSMLIGVPLGIIAGLKPASFKARSIMTSSVLGFSLPNFWVGMMFIMLFSVYLGW
                     LPAGGRGPVTTVGPIQLSLFTWEGLKSLILPAATIAVAKCALIIRVTRAATRECLPLD
                     YIKYARAKGLPERRVLGVHLLKNILIPIITIGGLEYGQVFAFAVVTETVFSWPGMGKL
                     LIDSIITLDRPVVVAYLMLAVVFLAVLNLVVDIAYTILDPRVRMGGPS"
     gene            2870..3805
                     /locus_tag="VIR04_05985"
     CDS             2870..3805
                     /locus_tag="VIR04_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460331.1"
                     /translation="MTVADEAAKTAPAVGSRVAPRESRWHIFVAQFVASKLAVFGLVL
                     LVVFLFLAIFAPWVSPQNPFDIATLDIFDSKLPPGSVSGDGSMTYWLGTDGQSRDIYS
                     AILYGMRTSLIVGTVSVSVAFLIGAVVGLIAAYFGGRIDSVLMRIVDIQLSFPAILLA
                     LILLAVLGKGVDKVIYALVAVQWAYFARAARGAAIVERNREYVEAAQCLSLPWQRILW
                     RHIFPNCIPPLMVIATIDLAHAIALEATLSFLGVGVPVTEPSLGMLISNGFEYMLSGT
                     YWISFFPGIALALLVISINLVGDHLRDILNPRNVI"
     gene            3815..4810
                     /locus_tag="VIR04_05990"
     CDS             3815..4810
                     /locus_tag="VIR04_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460332.1"
                     /translation="MSTELTPSADALLLDVRGLKTWFYTREGVVKAVDGVSLQLREGE
                     ILGLVGESGSGKSMTAFSLMNLVDEPGRIQADRLFFNGHDLLTLSAEQYRSLRGKEMA
                     MIFQDPMMTLNPTLRIDTQMIEAVRAHEHVSAAAARVRSVEVLGKVGIPSPKDRMRVY
                     PHHLSGGMRQRVAIAIALLNAPKLIIADEPTTALDVTIQGQILYEMQKLCREMGTALI
                     WITHDLAIVSGLADRLAVMYAGRIVETGNTADIVRAAQHPYTHGLIASIPSVQTRGRE
                     LFQIPGATPSLLNLPEGCPFRSRCYRATDVCKQAPELRPVGPAHEASCWHPGKAP"
     gene            4807..5886
                     /locus_tag="VIR04_05995"
     CDS             4807..5886
                     /locus_tag="VIR04_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460333.1"
                     /translation="MTVAGKKPEVILGLRHLEKRFVQPMGIVERAGNLIQGHARPRSV
                     LAVADVDLDIYAGEVVGIVGESGCGKSTLGRMLAGILAPTHGEIYYRGQPVAQMNAAA
                     RRQYELGVQMIFQDPFSSLNPRMRVLDIIGEAPMVHGLIRRGEKEAYVSDLMQKVGLD
                     PAYRFRYPHQFSGGQRQRIGVARALALKPSVIVCDEAVAALDVSIQAQVLNLFIQLRK
                     EFALTYFFISHNLGVVSHVSDRVAIMYLGRIVELAQTETIFSAANHPYTQALIKELPQ
                     LRPDRRQFDPIKGELPSPIDPPPGCAFHPRCPYAMPRCRVERPRLVNLTSAARPGEAP
                     RPDHWSACHLNDMEAGASGEAGVQS"
     gene            complement(5877..6416)
                     /locus_tag="VIR04_06000"
     CDS             complement(5877..6416)
                     /locus_tag="VIR04_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9460334.1"
                     /translation="MARLPYADLKQADARPLVEQIIAERGSVLHLYQMLLHSPAIARG
                     WLNHLTAIRHLSTLEGKLREMVIMRVAILNGAPYEADQHAPIALKEGMRQAQLDELGQ
                     WEGSTLFNEKERAILAYTDAMTLNVQVPKETFDAVGKYFEPKQLVELTATIAAYNMVS
                     RFLGALDIHSDDERDINHD"
     gene            6523..6780
                     /locus_tag="VIR04_06005"
     CDS             6523..6780
                     /locus_tag="VIR04_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY9460335.1"
                     /translation="MGLTSAYAANAQTPQASSSGEVRSVDSAAGKITIKHGAIADLGL
                     PAMTLVYRIDPSLLAGIKPGDKVTFTATRENGKYVVIAIKK"
     gene            complement(6814..8103)
                     /locus_tag="VIR04_06010"
     CDS             complement(6814..8103)
                     /locus_tag="VIR04_06010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY9460336.1"
                     /translation="MSPHPPHALPSYLNADDLGPWGIYLEQVDRVAPYLGKLEKWLET
                     LKRPKRTLIVDVPIELDNGSVAHFEGYRVQHNTTRGPGKGGVRFHQNVTLAEVMALAA
                     WMSIKSAAVNLPFGGAKGGIRIDPRNFSMAELERITRRYTSEIGVIIGPNKDIPAPDV
                     NTNAQTMAWMMDTYSMNVGATTTGVVTGKPVSLGGSLGRVEATGRGVFVVASEAAHDA
                     GISLEGARVIVQGFGNVGGTAARLFYEAGAKVIAVHDHTGCVHNANGLNVLKLLAHVE
                     EHGGVAGASGAESLSPAEFWALDTEILIPAALEGQITRQNADSMRTKIIVEGANGPTT
                     PEADDILTSKGVIIVPDVLANAGGVTVSYFEWVQDFSSFYWTETEINHRLEAMMRAAY
                     ASTSAVAKEHNVSLRTAAFIVACTRILESRVVRGLYP"
     gene            8204..>8395
                     /locus_tag="VIR04_06015"
     CDS             8204..>8395
                     /locus_tag="VIR04_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY9460337.1"
                     /translation="MSESSMAGRLAHVRERMAGACSRAGRLIDEVALLPVSKTFGPDA
                     VRAARALGLTRFGENKTQEI"
BASE COUNT         1611 a         2262 c         2559 g         1963 t
ORIGIN      
        1 ccagccgata ataggctcga ttaacttcat gatggtgaat cctgtgatac gcggaggaat
       61 agcattgcgt tgccgccagc gtagcaccaa atttgtaaaa tcgggaaaac actaatcata
      121 aagcccgccg gccccggtac actcggccga tgtcattcag tttcttatat gcatttggcg
      181 aatcgcttat acataattgt catgattcgc ctcgaccgct tgatgtattg taatgcccgg
      241 cgtttcatgg cttcccacta tgcattggct tagttgccaa atttttataa ggctgcaaaa
      301 tcatgaagtt gatacgtaaa acaattctgt tgtcggcgtt ggcgctgagt tttgccggtg
      361 tcaacgggct ggcgcaggcg cgcgacctga cggtcgcttt acgcaccgaa cccagttcga
      421 tggatccgct gttccattcg ttgacgccca atatacagat gtccgaaacg atttttgacc
      481 ctctggttcg caccgacgcc aatgccaaac cgatacctgc tctggccgaa tcctggacgg
      541 tggacggcaa cgtctggctg tttaaattgc gcccgaacgt caagttttct gacggctccc
      601 cgttcacggc agacgacgtt gtgttcacat acgcccgtgt gcccaaggtg cccaacagcc
      661 cttcgtcatt ttccttgttt ctaagttcga ttgccaaggt cgaggccgtc gattccctga
      721 cgctgaaaat caccaccaag ggcccgtcgc ctgtcttgct ggccaatctg tcgcaagtat
      781 cgatcatgtc gaaaaaggcc gcgtggggat cggcccccga aggtaaaact accgtcgagt
      841 taaaccgggg cgagggcctg gtgggaacgg gcccatacaa attcgtatca tggaagcgcg
      901 gcgacgaaat tgttcttgcg cgaaacgacc actattgggg caagaagccc gcctgggaca
      961 aggtcattta ccgccccatt tcgaatccgg ccgcgcgggt ggccgcgctt ttggcgggcg
     1021 acgttgattt ggtggaagat cctcccactg acgacctgcc ccggctgacg aagaacaagg
     1081 ctctgaccgt tcaggaaacg ccttcgctgc gcgttatata tttggccatg aaccagtcca
     1141 aagaagtgcc cccgggcatg acgggcacca acggcaagaa tgcgcttcag gacaagcgcg
     1201 tgcgcgaggc gttatcgctg gcgctggatc gcaaggcgat cgtggcgcgc atcatgggcg
     1261 gggttgccaa acccgcggcc aacttgctgg cctatcccgg atttggcgcg tccaaaaagc
     1321 tttccgaagt tcccgcaagc gatcccgtca aagccaaaaa attgctgacc gaggccggct
     1381 atcccaacgg gttttcgatt tcgctgggat ccccggcggg ccgctacacg aatgacaaga
     1441 atatcgctca ggcggcggcg gccatgtggg cccgaatcgg cgttaaaacc gatgtgcaga
     1501 cgatggcccc gccggtgttt ttcaaacagc gcgatacata cgctttcagc acctatctgg
     1561 gcggctgggc ggtgacttcc ggcgaaatgc tgaatccctt gaatgctctg gtcgtgacgc
     1621 ccaataaaga gctgggcctg ggcaccacca atcgcagcca ttactccaat ccagaactcg
     1681 acaaggtagt gcttcaggca tcaaaaacgc ttgatgacgg gaagcgatcg gaactgctgc
     1741 agaaagcagg aaatatggcc atggaagatt atgccatcct gccggtgcat ttcgaactgt
     1801 cggtgtgggc aatgaaaaag gatgttcact atgccgggcg cgcggaccaa atgacgctgg
     1861 cgcaagacat gacgcccgcc aaatagtgca ttgacaggat gttttcggcc attgtccggc
     1921 gcctgttgca gacggttgtc gtcgttttgg cgatgtccgc gctggtgttt gccgggctgt
     1981 atctggtcgg ggaccctgtc tcgatgctgg ccagtcccga ggccaccgat gctcagcgag
     2041 agctgatccg gcagtctttg gggctgaatc tgcccttgtg ggagcagtat ttcatttttc
     2101 tgggtaaagc ggctcacctg gatttcggca acagcttttt aaccggcgag tcggcgatgc
     2161 ggctgattct ggatcgcatg ccggccacgc tggagctggc cacgcttgcc atgttcatgt
     2221 cgatgctgat cggcgtgcct ttgggcatta ttgcggggtt aaagcccgca agtttcaagg
     2281 cgcggtcgat catgacaagc tcggttcttg gtttttccct gccgaatttt tgggtaggga
     2341 tgatgttcat catgttgttt tcggtctacc tgggctggct tccggcgggc gggcgcggac
     2401 ccgtgacgac cgtgggcccc attcagctca gcctgtttac ttgggaaggt ttgaaaagcc
     2461 tgattttgcc ggcggcaacg atagccgtgg ccaagtgtgc gctgatcata cgggtcacgc
     2521 gggcggccac gcgcgaatgc ctgcctctcg attacatcaa gtacgcgcgc gccaagggat
     2581 tgcccgagcg gcgggtgctg ggcgtgcatt tgctgaagaa tattttgatt ccaatcatca
     2641 ccataggcgg ccttgaatat ggccaggtgt ttgcttttgc cgtagtcaca gaaaccgttt
     2701 tctcgtggcc cggcatgggc aagctgctga tcgattcgat catcacgctt gaccgccccg
     2761 tggtggtcgc ctatttgatg ctggcggtgg ttttcctggc ggtcctgaat ctggtggtgg
     2821 atatcgccta cacaattctg gacccgcgag tgcgcatggg agggccatca tgacggttgc
     2881 cgacgaagcg gccaagaccg cgcccgccgt cgggtctcgc gttgcaccgc gtgaaagccg
     2941 ctggcatatt ttcgtggcgc agtttgttgc cagtaaattg gcggtattcg ggctggtatt
     3001 gctggtggtg ttcctgttcc tggccatttt tgcaccgtgg gtgtcgccgc aaaacccgtt
     3061 tgatatcgcc acgcttgaca tattcgattc caagttgccg ccaggcagtg tcagcggcga
     3121 tggctccatg acgtattggc tgggcaccga cggccagtcg cgcgatatct attccgccat
     3181 tttgtatggc atgcgcacca gtctgatcgt cggcacggtg tcggtgtcgg tggcgttttt
     3241 gattggggcg gtcgtcgggt tgattgccgc ctatttcggc ggacgcattg attccgtttt
     3301 aatgcgcatc gtcgatatcc agttgtcgtt cccggcgata ttgctggccc tgattctgct
     3361 ggcggtgttg ggcaagggcg tcgacaaggt catttacgcc ctggttgccg tgcagtgggc
     3421 ctattttgcg cgcgccgcgc gcggtgccgc catcgtggaa cgcaatcgcg aatatgtcga
     3481 ggcggcgcaa tgtctgtcgc tgccctggca gcgtattttg tggcgtcata tttttccgaa
     3541 ttgcattccg ccgctgatgg tgattgccac cattgatctg gcgcacgcca tagcgctgga
     3601 ggccaccttg tcttttctgg gtgtcggcgt gccggtgacc gagccttctt tgggcatgct
     3661 gatttccaac ggttttgaat acatgctgtc gggcacctac tggatttcgt tttttcccgg
     3721 cattgcgctg gcgctgctgg tgatctccat caatttggtt ggcgaccatc tgcgcgacat
     3781 tcttaacccg cgcaatgtta tttgaggaac ttgattgagc accgaactta cgccatcggc
     3841 cgatgccctt ctgctggatg tgcgcggctt gaaaacctgg ttttatacgc gcgagggcgt
     3901 ggtgaaagcg gtcgatggcg tctcgctgca gcttagggaa ggtgaaattc tggggctggt
     3961 tggggaatcg ggatcaggta aaagcatgac ggccttttca ttgatgaatc tggtcgacga
     4021 gccaggccgc attcaggcgg acaggctttt cttcaacggc catgatttgc tgacattgtc
     4081 ggccgagcaa tatcgatcgc tgaggggcaa agagatggcg atgatttttc aggatcccat
     4141 gatgaccctg aatccgacgc tgcgcattga tacgcaaatg atcgaggccg tgcgcgctca
     4201 cgagcatgtg tcggcggccg ccgcccgggt tcgttcggtg gaggtcttgg gcaaggtcgg
     4261 aatcccgtcg ccgaaagacc ggatgcgcgt gtacccgcat catttgtctg gcggaatgcg
     4321 acagcgtgta gcaattgcca ttgctttgtt gaatgcgccc aagttaatta ttgcggacga
     4381 gcccacaacg gcgctggacg tgacgataca gggacagatc ttgtacgaaa tgcaaaaatt
     4441 gtgccgtgaa atggggacgg cgcttatctg gattacgcac gacctggcga ttgtgtcggg
     4501 tctggcggat cgtctggccg tcatgtatgc gggccgcatt gttgaaaccg gcaatacggc
     4561 agacatcgtt cgggccgctc aacatcccta tacccatggc ctgatcgcct cgattccttc
     4621 ggttcagacg cgtggccgcg agttgtttca gatcccgggc gcgacgcctt ccctgttgaa
     4681 cttgcctgaa gggtgcccgt tccgatcgcg gtgttatcgc gcgaccgatg tctgcaaaca
     4741 ggcgcccgag cttcggcccg tggggccggc gcacgaggca agttgctggc atccggggaa
     4801 ggcgccatga cagttgccgg caagaagccc gaagtcattc ttgggctgcg tcatcttgaa
     4861 aagcgtttcg ttcagcccat gggcatcgtc gagcgtgccg gcaacctgat tcagggccat
     4921 gcgcggccaa gatcggtgct ggccgtcgcg gacgtggatc tggatattta cgcgggcgag
     4981 gtggtgggca ttgtcggcga gtccggttgt ggaaagtcga cgctgggccg catgctggcg
     5041 gggattctgg cgcctaccca tggcgaaatt tattataggg gtcagcccgt tgcgcaaatg
     5101 aatgcggccg cgcggcgcca gtacgaactg ggcgtacaga tgatttttca agaccccttt
     5161 tcgtcgctga atccgcgcat gcgggtactg gacattatcg gcgaagcgcc gatggtgcat
     5221 gggcttatca ggcgcggcga aaaagaagcc tatgtttccg atctcatgca aaaggtgggc
     5281 ctggatcctg cctatcggtt tcgctatccg catcagtttt cgggcggtca gcggcagcgt
     5341 attggcgtag ctcgcgcgct tgctcttaaa ccgtccgtga ttgtgtgcga cgaagccgtt
     5401 gccgcgcttg acgtctccat tcaggcacaa gttctgaatt tattcattca acttcgcaaa
     5461 gaatttgccc tgacctattt ctttattagt cataatctgg gcgtggtgag ccatgtttcc
     5521 gatcgcgtgg ccatcatgta tctgggccgg atcgtcgagc ttgcccagac ggaaactatt
     5581 ttcagcgcgg ccaatcaccc ctatacgcag gcgctgatca aagaattgcc gcagttgcgg
     5641 ccggatcgcc gtcagtttga tccgatcaag ggtgaactgc cttcgcccat tgatccgccc
     5701 ccggggtgcg cattccatcc gcgctgtccc tatgccatgc cgcgttgccg ggtggaacgg
     5761 ccgcgtcttg taaacctgac aagcgctgcc cggccgggtg aagcgccgcg gcccgatcat
     5821 tggagcgcct gccatttgaa cgacatggag gcgggcgcgt ctggcgaggc aggcgttcaa
     5881 tcgtgattga tgtcgcgttc gtcgtcggag tgaatatcaa gagcgcctag aaagcgtgag
     5941 accatgttgt aggcggcaat cgtagcggtg agttcaacca attgtttggg ttcgaaatac
     6001 tttccaacgg cgtcaaatgt ttctttcggc acctgcacgt taagggtcat ggcgtcggtg
     6061 taagccagaa tcgcacgttc tttctcgtta aacagcgtcg agccttccca ttgacccaat
     6121 tcatcaagct gggcttggcg catgccttct ttgagggcga tgggcgcatg ctgatccgct
     6181 tcgtacggcg cgccgttcaa tatggcgaca cgcataatga ccatctcgcg caacttgccc
     6241 tcaagcgtgc tcaaatgtcg aattgcggtc agatggttta gccagccgcg cgcgatggcg
     6301 ggactgtgca gcagcatttg gtacaggtgc aaaacgctgc cgcgctcggc aataatttgt
     6361 tcgaccaatg gacgggcgtc ggcttgtttc aggtcggcgt agggaaggcg ggccatattt
     6421 gtcctttcag gaaaagaaca tgaattgtaa ggaacttatt ttataaggag cagcttaatg
     6481 agtatgacga atcgtcgatt gcagcaagcg ctggtcgcgg ggatggggtt gacgtcggct
     6541 tatgcggcca atgcgcaaac cccgcaggcc agctcttcgg gcgaggtgcg cagcgttgac
     6601 agtgcggccg ggaagataac gatcaaacat ggcgctatcg ccgacctggg cttgccggcc
     6661 atgacgctgg tttatcgtat tgacccttca ttgctcgcgg gcatcaagcc gggcgataaa
     6721 gtcaccttta ccgccacgcg cgaaaatggc aagtatgtcg tgattgccat caaaaaatag
     6781 tgacgcttca gtggcggcgc ccttcggccg gaatcagggg tacaggccgc gcacgacacg
     6841 ggattccaga atgcgagtgc aggccacgat gaatgcggcg gttcgcagcg agacgttgtg
     6901 ttccttcgcc acagccgatg tcgaggcgta ggccgcgcgc atcatggctt cgaggcgatg
     6961 attgatctcg gtttcggtcc agtaaaagct tgaaaaatcc tgcacccatt caaaatagct
     7021 gacggtgacg ccacctgcat tggccagcac gtcgggcacg atgatgacgc ctttggaagt
     7081 caggatatcg tcggcttcgg gggtggtcgg gccgttagcc ccttcaacga tgatttttgt
     7141 gcgcatcgag tcggcatttt gccgggtgat ttgaccttcc agcgcggccg ggatcaggat
     7201 ttcagtgtcc agagcccaga attcggcggg actcaggctt tcagcgccgg aggcgccggc
     7261 gaccccgccg tgttcttcga cgtgagccag cagcttcaaa acgttcaggc cgttggcgtt
     7321 atgtacgcag cccgtgtggt cgtggaccgc aatgactttg gcgcccgctt cataaaatag
     7381 ccgggcggct gtgccgccca cgtttccaaa gccttgcacg atgacgcgcg cgccttccag
     7441 tgaaatgccg gcgtcgtgcg ccgcttcact ggccaccaca aatacgccgc gccccgtggc
     7501 ttcgacgcgg cccagactgc cgcctaacga aaccggcttg ccggttacaa cgcctgtggt
     7561 ggtcgcgcct acattcatgg agtaggtgtc catcatccag gccatggttt gcgcgttggt
     7621 gttgacgtcg ggcgccggga tgtccttatt gggtccaatg atgaccccga tttcgctggt
     7681 gtagcggcga gtgatgcgtt ccagttcggc catggagaag ttgcgcgggt cgattcgaat
     7741 gccgcctttg gcgccgccga atggaaggtt gaccgccgct gattttatcg acatccaggc
     7801 ggccagcgcc atgacttccg caagagtgac gttttgatga aaccgcacgc cgcccttgcc
     7861 ggggccgcgc gtggtgttgt gctgcacgcg atagccctcg aaatgcgcga ccgaaccgtt
     7921 gtcgagctcg atcggaacat cgacgatcag ggtgcgtttg ggtcttttca gggtctcgag
     7981 ccacttttcc agcttgccaa ggtagggggc gacgcggtcg acttgttcaa ggtaaattcc
     8041 ccaggggcct aaatcgtcgg cgttcagata agaggggagt gcgtgtgggg ggtgtggtga
     8101 cattgctttg gcctccgggc attgaaagac actgttgcaa agacaatccg gattgtaagc
     8161 cttacactct tcagtagtgc aaacaaatag gaatggtgga cggatgtccg aatcttcaat
     8221 ggcaggtcgc ctggcgcacg tccgcgagcg catggcgggc gcttgctccc gggcggggcg
     8281 gctgatcgat gaggttgcct tgctgcccgt cagcaaaaca tttgggcctg atgcggtgcg
     8341 tgccgcccgg gcactgggtt tgacacgttt tggcgagaat aaaacgcagg aaata
//
LOCUS       DATCXU010000268         6669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_644539,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000268 DATCXU010000000
VERSION     DATCXU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644539"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1323
                     /locus_tag="VIR04_06020"
     CDS             157..1323
                     /locus_tag="VIR04_06020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9460338.1"
                     /translation="MHFELTPRQRQVYDTVGELSRTVFKPNAAKHDTDASTPRENLKA
                     LFEAGFNGITISESFGGMGSGAVGTDPLLYLLAVEQTARGDLSTAQCFHIHCHGAHYV
                     DQVGTEEQRERVLRPVLESGSLLNATGSEPGRTSRGAYNLVTSAERAEGGYVLNGMKN
                     YATLGADVDYNLIFAGIPDKPAGQGHLSVLVPQDANGFSVIDGSWNPMGMRGACSPSL
                     KLDNCFVDDRNVLGDLGIYPRERWQARFHLSFAAQYLGGAEAIFDLLCEYLPKRGTAS
                     DSYTQLRLGEIRVGTESVRWLIYRAAWLWTQGNREAAELSSICAKHRALENAVIAMDK
                     GAQIVGSSAFWADSAMSRLFRDLRIHTLHENLDTTAAILGKYHLGQPYDTTARL"
     gene            1342..1818
                     /locus_tag="VIR04_06025"
     CDS             1342..1818
                     /locus_tag="VIR04_06025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY9460339.1"
                     /translation="MKLNEREFRNALGRYPTGVTVICTHTPDGDLVGLTANSVTAVGA
                     GPVSLAWNLGVASRTRAIFEAADYFAVNVLAGSQVDVARQFAAPVANRYESINYSVKG
                     PCGLPKINGVIAWYACRRTGIVEVGDHIMFLGDVVESEVADGAPLLYFAGQFAQAH"
     gene            1837..3237
                     /locus_tag="VIR04_06030"
     CDS             1837..3237
                     /locus_tag="VIR04_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9460340.1"
                     /translation="MPNFGQASTAELLNQLPPELIDIGADTSRPFWNMGRETMPFEQM
                     RALQTAKLQRQVAYLGKYSEFYKRKFQSAGFDHNACATIKDISAVPFTTKAELRDSQA
                     AHPPFGLHQAASMDDIVRVTATSGTTGKSVFQGYTAHDTLRRSESLARVLWGFGVRPG
                     DRVINGFALSMFNAGIPLCAAVERLGAVNIPAGAERRVEGMLTLMQQLKATVWVGTPS
                     FAAALAERCTEVLGVAPQELGLRILCGGGESGFEQPAFQKRMEEAWGTPHVFDFASTS
                     DAHPNVFAHCQHRDGKHHLTPDLALIELIDPATGAILEFTDGIEGEYVFTHLDRQACP
                     LFRYRTGDIIKVRTSKCACGRTGFRMDIVGRSDDMLIVRGINVFPSALQSVISTFSPQ
                     MSGAIQVVLSKPGPAVIPPLRIRVEAVGANKELKTALEQVIRRALTVSVDIEFVPVGT
                     LGRTEKKSKLIVLEEL"
     gene            3234..3722
                     /locus_tag="VIR04_06035"
     CDS             3234..3722
                     /locus_tag="VIR04_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY9460341.1"
                     /translation="MSQPLMQRGQPRAKHRLACGLVMVGYVDACDKPRSVMASSFNSV
                     SLTPRLVMWCISNERIHGSEIGVGTKCGLSVLAADHHERWAQYANDCDMHRFEWTKGV
                     ALGVPLLMNAAACFEVEIRRRIDQKSGVLYVGEVNSFVYSETSKSLFSYLDDVGVPRV
                     NT"
     gene            3751..5145
                     /locus_tag="VIR04_06040"
     CDS             3751..5145
                     /locus_tag="VIR04_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460342.1"
                     /translation="MKMPGDETTPTLSEIIAQHVSTTQFSSLPEHTLVAAKRLILDTL
                     AVAWAGSGSTGIGAVSAALSSSENRIAGANGEKCTLWGTAEQASVQNAAFINGAAAAA
                     LDYDALHLNALVHSSIVTLPPLLALSEHLHLDGKALLTALVLADDINCRLGLSAQGHN
                     GWFFTSIFGVFGTAAACAKLLGLDQAGIANALGIALFQVTGTQQSMIEKKLTKRFMSA
                     FAARGGVFSAMLAQAGVDAPRRPFEGEFGLFRLYQKCDAEILLEDLGTRYENSFISIK
                     KFPSCGCAHALTEAALLIVERHRILPKDIARVTVRISEFMNRLVGGQFNPEQDGPQVT
                     GQFSAQYAVACVIFRKRFIVDDIQSANVMEHEVANFARSVVIEVDAENTGQLVPAEVI
                     FEMRSGAKYIENVARFPGGESGAISKNILHDKVYDCMRQGPRPLIPEQIERLINRVEG
                     LETMSDMADLFKNL"
     gene            complement(5204..5920)
                     /locus_tag="VIR04_06045"
     CDS             complement(5204..5920)
                     /locus_tag="VIR04_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460343.1"
                     /translation="MLNPVLRISDLDVSYGKIPALVDIDIELFQGEAVGLLGANGAGK
                     TTLLNTLSGFLRPTAGAVELFGNAIDRCQPHDIVRRGLSHLSQERDLFGDLTVLDNLR
                     LGSLARASKSFDRNVERVFHFFPRLKERATQYAATMSGGEQQMLAIGRALMAEPKVLL
                     FDEPSAGLSPLFVQEIGAMMQGLRKEGEITMILVEQNMLLAAQVIDRFYMLRAGRVVA
                     QGPVSELEKDHEELAREYYL"
     gene            complement(5913..>6669)
                     /locus_tag="VIR04_06050"
     CDS             complement(5913..>6669)
                     /locus_tag="VIR04_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460344.1"
                     /translation="TMLEIQNLTVKFAGLTAVDDLSFSVQAGEIFTLMGPNGAGKTTA
                     FNAIGGFVQSAAGQVFFNGQNISGMAPEHVAALGIRRTFQNNGILREMSVLENVLVGL
                     ELSTASSFWGCVTGWPSSTHAERGAVRLSREILQEMEIAELADRTAGDLSFGQQRLVE
                     IARAMVAGAKLIMLDEPAVGLSPGERLHLGQVLRQMSANGMAILLVEHVQDLVMAVSD
                     QVLVLNYGKKIAQCPPDEIRQNKDVLEAYLGYA"
BASE COUNT         1523 a         1712 c         1907 g         1527 t
ORIGIN      
        1 cagccgacgt cccagagtcg gttctccagt cctggccaac atagcgaaac ggttaatcga
       61 gtttcattat gttgatcatt atttgaaatg cgcttgataa cataatagcg aatatagcaa
      121 tacgttgtat ctacttttta agatataggg aacacaatgc attttgaact cacaccgcgt
      181 cagagacagg tatacgacac cgtaggagag ctcagcagaa cggttttcaa gccgaatgcg
      241 gccaagcacg ataccgatgc aagcactcca cgagaaaacc ttaaagcgct tttcgaggcg
      301 ggttttaacg ggataacaat ttccgaaagt tttgggggaa tgggaagcgg cgccgtcggc
      361 acagatcccc tcctctatct cctggctgtc gaacagacag cgcgcggaga cttatccacg
      421 gcacagtgct ttcacattca ctgccatggc gctcactatg ttgaccaagt cggcaccgaa
      481 gagcagcgtg aacgtgtgct tagacctgtg ctggaaagtg gaagtctttt aaacgccact
      541 ggcagtgaac ccggacgcac ctcaaggggc gcttacaacc tggttacttc ggccgagcgc
      601 gcggagggcg gctacgtcct gaacggaatg aagaattacg ccactttggg tgcggacgtc
      661 gactacaacc tgatttttgc cggaattccg gacaagccgg cgggccaggg tcacctgagc
      721 gttctcgtgc cgcaggatgc aaacggtttt tccgttattg atggttcatg gaatccaatg
      781 ggaatgaggg gagcgtgcag cccgtcgctc aagcttgata attgtttcgt ggatgaccgg
      841 aatgtgctgg gtgatttggg gatttatcct cgtgaacgtt ggcaagctcg gttccatttg
      901 agttttgccg ctcagtatct gggtggggcc gaagcgattt ttgatctgtt atgcgaatac
      961 ctccccaagc gcggcactgc aagcgattca tacacgcagc ttcgcctcgg tgagattcgg
     1021 gttggtacgg agtcggtccg atggttgatc tatcgggcag cttggctgtg gacgcaaggc
     1081 aatagagaag cggcggaact gtcgtcgatc tgcgcgaagc atcgcgctct tgaaaacgca
     1141 gttatcgcta tggataaggg cgcgcaaatt gtcggttcaa gcgcgttctg ggccgattcc
     1201 gcgatgtcgc ggttattccg ggatctgcgc atacatacct tgcacgaaaa tctggacacg
     1261 acggctgcga tactgggcaa gtaccatctt ggccagccat acgatacgac ggcgcggttg
     1321 taacggaggg tggagatcga tatgaaattg aatgagcgtg aattcagaaa tgctctgggc
     1381 cgttacccaa cgggtgtaac ggttatctgc acccatacgc cggatggaga tctggtgggt
     1441 ctaacagcta actctgtgac cgccgttggt gcgggccctg tcagccttgc atggaatctg
     1501 ggggtcgcat ccagaacacg cgcgatcttc gaagcggcgg actatttcgc cgttaatgtg
     1561 cttgcaggca gtcaagtgga tgtagcccgc cagttcgcgg ctccagtggc caatcgttat
     1621 gaaagcatta actattcggt aaaggggcca tgtggtttgc caaaaatcaa tggcgtaatt
     1681 gcgtggtacg cgtgccgacg gaccggtatc gtggaagttg gagaccatat tatgtttctt
     1741 ggtgacgtgg tcgaaagcga ggttgccgat ggcgctcctt tattatattt cgccggccag
     1801 tttgcccagg ctcattgagc aaccaaggac ggtgatatgc ctaattttgg acaggcttcg
     1861 acagcagagc ttcttaacca gttgccgccg gaattgatcg atattggcgc cgacactagt
     1921 cgtccgttct ggaacatggg acgggagact atgccattcg agcagatgcg tgcgcttcag
     1981 acagccaagt tgcagcggca agttgcgtat ctgggaaaat attcagagtt ttacaagaga
     2041 aaatttcagt cggccggttt tgatcataac gcttgtgcga ccatcaaaga tatttcggct
     2101 gtccctttca cgacgaaggc ggagctgcgt gatagtcaag cggcacaccc gcccttcggg
     2161 ctgcatcaag cggcatcaat ggatgacatt gttcgcgtca ctgcgacgtc cgggacgact
     2221 ggcaaatccg tatttcaagg ctatacggca cacgacacgc tgcggcgctc ggaaagcctt
     2281 gcacgggtgc tatgggggtt cggtgtacgt ccgggcgata gggtaatcaa tggctttgcg
     2341 ctgagcatgt tcaacgcggg aatccccctg tgtgcggccg tcgagcggct gggagcggtc
     2401 aacattcctg ctggagcgga gcggcgagtg gaaggtatgc tcaccctgat gcagcaactg
     2461 aaagcaacgg tgtgggtcgg cacgccttct ttcgcagcgg cgctcgccga gcggtgtacc
     2521 gaggtccttg gggtggcgcc ccaggagctc gggctccgga tactatgcgg cggaggggaa
     2581 tcgggcttcg agcaaccggc gtttcagaaa cgtatggaag aggcatgggg cacccctcat
     2641 gtattcgatt tcgcctcgac gtcggatgcg caccctaatg tattcgccca ctgtcagcat
     2701 cgcgacggca agcaccatct gacgcccgat ttggcgttga tcgaactaat cgacccggca
     2761 acgggcgcca ttcttgaatt cacagatgga atcgagggag agtacgtatt cacacacctg
     2821 gataggcagg catgccccct attcagatat cgcacgggcg atatcatcaa agtgcgtacg
     2881 tcaaaatgcg catgtggccg cacagggttt cgcatggaca ttgtcggccg aagcgacgat
     2941 atgttgattg tgagaggaat caatgtattt ccgtcggcgc tgcaatcggt tatcagcaca
     3001 ttttccccgc aaatgagcgg cgcaattcag gtcgttttat ctaagccagg gccagccgtg
     3061 attccgccgc tgagaatccg ggtggaggca gtgggcgcaa ataaagaatt gaagaccgcg
     3121 cttgagcaag tcatccgccg ggcactcaca gtaagcgtgg atatcgaatt tgtaccggtg
     3181 gggacattgg gtcgtacaga gaagaaatcc aaacttatcg tactggaaga attatgagcc
     3241 agcccttgat gcaacgtggt caaccacgtg cgaaacatcg tttggcgtgt gggctggtca
     3301 tggtgggata cgtcgatgcc tgcgataaac cacggtctgt gatggcaagc tcgtttaact
     3361 cggtatcgct tacgcccaga ctggtgatgt ggtgtatttc aaacgagcgg atccatgggt
     3421 cagagattgg ggtgggcaca aaatgcggct tgagtgtttt ggctgcggat catcacgagc
     3481 ggtgggctca atatgcaaac gattgcgaca tgcatcgctt tgaatggaca aaaggcgtgg
     3541 cgctgggcgt tccgctttta atgaatgccg cggcctgctt tgaggtggag ataaggcggc
     3601 gtatcgatca aaagtcgggc gttctttatg ttggcgaagt gaattcgttt gtgtattccg
     3661 aaacatctaa aagtctattt agctatcttg acgacgttgg tgttccgcga gtgaacacat
     3721 agaatccgtc aataattttt gaggcgcgta atgaagatgc ccggagatga aaccaccccc
     3781 acgctgtcag agataatcgc gcaacacgtt tcgacgacgc aattttcgtc gctccccgag
     3841 cacacacttg tggcggcaaa aagactgata ctggatactt tagcggtagc gtgggccgga
     3901 tcgggttcga cggggatcgg cgcggtcagc gcggcactaa gctcatccga gaacaggatc
     3961 gctggagcaa atggcgagaa atgcactcta tggggtacag cggagcaagc gtcggttcaa
     4021 aatgccgcat tcatcaatgg cgcggccgct gccgctcttg attatgatgc gctccacctt
     4081 aatgcactgg tgcattcgtc aatcgtcact ctgccgccat tgctggcgct gtcagagcac
     4141 ctgcatttgg atggcaaggc cctgttgacc gcacttgttc tggcagacga tattaactgc
     4201 cgccttgggt tgtccgcaca aggccataac ggctggtttt ttacctcgat ctttggcgtg
     4261 ttcggcaccg ccgcagcctg cgcaaaacta ctgggcctcg atcaggcagg aattgccaac
     4321 gccttgggca ttgcactgtt tcaggttact ggcacacagc agtcgatgat agaaaaaaag
     4381 ctgaccaagc gctttatgtc cgccttcgcc gctcggggtg gcgtgttttc tgcaatgttg
     4441 gcccaagcgg gtgtcgacgc gcccaggagg ccttttgaag gagaattcgg tctttttaga
     4501 ctgtaccaga aatgcgatgc agaaattctg cttgaagacc tgggcactcg ctacgagaac
     4561 tcctttatca gtatcaagaa attcccgagt tgcggatgcg cgcatgcctt gaccgaagcg
     4621 gcattattaa tcgtcgagcg gcatcgaatc cttccaaaag atatcgctcg tgtaacagtg
     4681 cggatttccg agttcatgaa tcgcctggtg ggcggtcaat tcaaccccga gcaagatggc
     4741 ccgcaggtta cgggtcaatt tagcgctcaa tacgcggtgg catgtgtgat cttccgaaag
     4801 cgatttattg tggacgacat ccaatccgcc aacgtgatgg agcacgaggt cgctaatttc
     4861 gcgcgatccg ttgtgataga ggtggatgcg gaaaataccg gacagcttgt tccggccgaa
     4921 gtgatttttg agatgcgctc tggagcaaag tatattgaga atgttgcccg cttcccgggc
     4981 ggggaaagcg gcgccatctc aaaaaacata ttgcatgaca aggtttatga ctgcatgcgg
     5041 caggggccca ggcccttgat tccggaacag atcgagcgct tgattaatcg tgttgaaggg
     5101 ttggaaacaa tgagtgatat ggccgatctg ttcaaaaacc tttgaaatac cggcatcggg
     5161 ggacagacat tggttggccc agcgccgcct tatgcgatcc taactataaa taatattcac
     5221 gcgccagttc ttcgtgatcc ttctccagtt cactgacagg tccttgtgcc actactcttc
     5281 cggccctaag catgtaaaag cgatcgatga cttgcgctgc aagcagcata ttttgctcga
     5341 ccagtatcat ggtgatttca ccttctttgc gaaggccctg catcatagcg ccgatctctt
     5401 gcacgaacaa tggcgagagc cccgcggaag gctcatcgaa tagcaggact ttaggctcgg
     5461 ccatcaaggc gcgcccgatg gccagcattt gctgttcgcc gcccgacatg gtcgcggcgt
     5521 attgggtggc tcgttctttc aggcgcggaa agaaatgaaa cacacgctca acattgcgat
     5581 cgaatgactt ggaggcgcgg gccagagagc ccaagcgcaa gttgtccagc acggtcaggt
     5641 cgccaaacaa gtcgcgttcc tgcgatagat gcgacaggcc ccggcgcacg atatcgtgcg
     5701 gctggcaacg atcaatggcg ttgccgaaca attccaccgc gcctgcagtg ggacgcaaaa
     5761 agccggacag ggtatttaac aacgtggttt tgccggcgcc gttggccccc agcaagccaa
     5821 cggcttcgcc ttgaaacaac tcgatatcga tatcgaccag agccgggatt ttgccgtagc
     5881 tcacgtcaag atcggaaatt ctaagtacgg gattaagcat agccaagata tgcctccaga
     5941 acgtctttat tttgacgaat ttcatccggc ggacattgtg cgattttctt gccgtagttc
     6001 agcaccagaa cctgatcgga caccgccatc accaaatcct gcacatgctc gaccagcaaa
     6061 atcgccatgc cgtttgccga catttgccgc agcacctgtc ccaagtgcaa gcgttcgccg
     6121 ggggataatc ccaccgctgg ctcgtcgagc ataatcagtt ttgcaccggc caccatggcg
     6181 cgggcaattt ccaccaaacg ctgctggcca aaagacaaat cgcccgcggt tcgatcggcc
     6241 aactcggcga tttccatttc ctgaagtatt tcgcgcgaaa gccgaaccgc gccacgttcg
     6301 gcgtgtgtgg aacttggcca ccctgtgacg caaccccaga atgaactggc ggtggaaagc
     6361 tccaggccca ccagcacatt ttcaagcacg ctcatttcac gcaggattcc attgttctgg
     6421 aacgtgcgcc gaatgcccag ggccgcgacg tgttcggggg ccatgccgct gatattctgg
     6481 ccgttaaaaa acacctgccc cgccgccgat tgcacaaaac cgccgatcgc gttgaatgcg
     6541 gttgtcttgc ctgcgccgtt gggccccatc aaggtgaata tttcacctgc ctggacgctg
     6601 aacgacaagt cgtccaccgc cgtcaggccg gcaaatttga ctgtcaggtt ttggatttca
     6661 agcattgtc
//
LOCUS       DATCXU010000269         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_774543,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000269 DATCXU010000000
VERSION     DATCXU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774543"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VIR04_06055"
     CDS             <1..1192
                     /locus_tag="VIR04_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY9460345.1"
                     /translation="KVIAPIEDTPAARAGILAGDLITAINGKPTKGMTLNDAIKLMRG
                     EPKTSIVLTIKRQGKDKPFTVKIVRDLIKVRSVRSKMLDHNIAYARVAQFQEHTSEDL
                     TKQLRALGEKGTPKALILDLRNDPGGLLDSAIGVSSSFLPPKSLVVSTKGRIRSSNHE
                     YFAKSDSADVAPGSGSASDAWLKTVPMVVLVNVGSASASEIVAGALQDHKRAKIIGNR
                     TFGKGSVQSVLPLSDDTGIKLTTARYYTPNGRSIQVTGVEPDITVDDTAQGNLFRLPR
                     EVDLSHHLLNSSVASETPDDMTPDKDKTEPKMFEFGGKDDFQLKQAINYLEGRTVKKA
                     DAKKADAELASNTPAKAPPSSKKNGAKSSSSAHKAEKKNEAPPKKPAVEHFRITPDGL
                     VPAK"
     gene            1205..1954
                     /locus_tag="VIR04_06060"
     CDS             1205..1954
                     /locus_tag="VIR04_06060"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY9460346.1"
                     /translation="MDDMQLLRYARHILLDEIGIEGQERLMAARVLIIGAGGLGSPAA
                     CYLASSGVGHIVLADDDEVELSNLQRQILHTTARIGWPKAESGKQMLSEINPESQVQA
                     RVQRLTGTALDQLVAEVDLVLDCCDNFATRHEVNRACVAHRKPLVSGSAIRFAGQVSV
                     FDLRDDASPCYHCLFPQEDGVRELRCAETGVLAPLVGIIGSMQAAEAIKILAHIGEPM
                     IGRLLCLDALSMQWNEVRFARDPVCMVCSDR"
     gene            complement(1974..>2348)
                     /locus_tag="VIR04_06065"
     CDS             complement(1974..>2348)
                     /locus_tag="VIR04_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY9460347.1"
                     /translation="RKIVLTNAPLKYTREVLKTLKILHCFEGVWSIDRMRLQGRIRPK
                     PSPALMKQVSARVGAPAQRIVLIEDTLKNLKSARQAGMQTMYVFHPGTPFSNQRSGRG
                     NYVDVRVNSIGKLLVGKHALHR"
BASE COUNT          503 a          634 c          702 g          509 t
ORIGIN      
        1 caaggtgatt gctccaattg aagacactcc cgccgcgcgc gccggtattc ttgcgggtga
       61 tctgatcacg gccattaacg gcaaacccac caagggaatg accctgaatg acgccatcaa
      121 gctcatgcgc ggcgagccaa aaacgtcgat cgtgctgaca atcaaacgtc agggcaagga
      181 caagccgttc accgtgaaaa tcgtgcgcga cctgatcaag gtgcgcagcg tgcgcagcaa
      241 aatgctggat cacaatattg cttacgcgcg tgtggcccag tttcaggaac atacatcgga
      301 agatctgacc aaacagttgc gcgcgctggg ggaaaaaggc acgcccaagg cgctgatact
      361 ggatttgcgc aatgacccgg gcggtttgct cgacagcgcc atcggcgtgt cctcgagctt
      421 cctgccgccc aaatcgctgg ttgtgtcgac caaagggcgt attcgttcgt cgaatcacga
      481 gtacttcgca aaatcggact ctgccgacgt cgcgccgggt agcggttccg cctcggacgc
      541 ctggctgaaa acggtgccca tggtggtgct ggtcaatgtg ggctcggctt cggcctctga
      601 aatcgtggcg ggggctttgc aggatcacaa gcgtgcaaaa attatcggta atcgcacgtt
      661 cggcaagggc tcggtgcaaa gcgtactgcc gcttagcgac gataccggca tcaagctgac
      721 cacagcgcgt tattacacgc ccaatgggcg ttcaattcag gttaccggcg tggagcccga
      781 tattacggta gacgatacgg cccagggcaa cttgttccgc ttgccgcggg aagtcgacct
      841 gagtcatcat ttgttgaata gctcggtcgc ttccgaaacc cccgacgaca tgacgccgga
      901 caaggacaag accgaaccca agatgttcga gtttggcggc aaggacgact ttcaattgaa
      961 gcaggcgata aattatcttg aaggacggac cgtcaagaag gcggacgcga aaaaggcgga
     1021 tgccgagctt gcgtcgaaca cgcccgccaa ggcgcctcca tcgtcaaaga aaaatggcgc
     1081 caaatcatcc tcatccgcgc ataaggctga aaaaaaaaat gaagcgccac ccaaaaagcc
     1141 cgctgtagag catttcagga ttacgcccga cggattagtg cctgccaaat agcgctttgt
     1201 tgtcatggat gatatgcaat tgttgcgcta tgcgcgtcat atcttgctcg atgaaatagg
     1261 catcgagggg caggagcgcc tgatggcggc gcgtgtcttg atcatcggag cgggcggtct
     1321 gggctcgcca gcggcatgtt atctggcttc ttcgggcgtg ggccatatcg ttctggccga
     1381 tgacgacgaa gtcgagctta gcaatttgca gcgtcagatt ttgcatacga cggctcgcat
     1441 cggatggccg aaagccgaat cgggcaagca aatgttgtct gaaatcaatc ccgagtcgca
     1501 ggttcaggcg agggttcaac gcttgactgg aaccgcgctc gatcaacttg ttgctgaagt
     1561 ggatctggtg cttgattgtt gcgataactt cgccacccgt cacgaggtga atcgggcctg
     1621 cgtggcgcat cgcaagccgc tggtttcagg gtcggccatt cgctttgcgg ggcaggtcag
     1681 tgtctttgat ctgcgcgacg atgcatcgcc ttgttatcat tgcctgtttc cccaagagga
     1741 tggcgtgcgc gagcttcgtt gcgcggagac cggggtgctc gcgccgctgg ttggaattat
     1801 cggcagcatg caggccgccg aagccatcaa gattttggcg cacataggcg aacccatgat
     1861 cggacgcctg ttgtgcctgg atgccctgtc catgcagtgg aacgaggtgc ggttcgcgcg
     1921 tgatcccgtt tgtatggtct gtagcgaccg gtaggcggtt tgagcccttg tcattagcgg
     1981 tgcaacgcat gcttgccgac gagcagcttg cctatggaat tgacccgcac gtcgacgtag
     2041 ttgccgcgcc cgctgcgctg atttgaaaaa ggtgttcccg ggtggaagac atacatggtc
     2101 tgcatgcccg cctgacgcgc gcttttaaga tttttcagcg tgtcttcaat tagcacaatg
     2161 cgttgtgccg gcgcaccgac ccgtgcggat acctgtttca tcagcgccgg cgacggcttg
     2221 ggccttatcc gcccctgcag gcgcatgcgg tcgatcgacc agacgccttc aaagcaatgc
     2281 aggattttca atgttttcag gacttcacgg gtgtacttca gcggtgcgtt ggtgagcact
     2341 atttttcg
//
LOCUS       DATCXU010000270         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_740537,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000270 DATCXU010000000
VERSION     DATCXU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740537"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VIR04_06070"
     CDS             <1..280
                     /locus_tag="VIR04_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741265.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY9460348.1"
                     /translation="QVAQRAGIPPAVIRQASRELSRLEAQGAPTPQLDLFSSVAGDLP
                     DEPAANDPDPATGPAGLTLKARLAAIEPDQLTPREALDILYHLRADFP"
     gene            277..1470
                     /locus_tag="VIR04_06075"
     CDS             277..1470
                     /locus_tag="VIR04_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9460349.1"
                     /translation="MNINRPLDLLGGLSPQAFMRHYWQRKPLLVRQAIPGFRPNLSIA
                     DIKQLVRRDEVESRLIRRDGNGWNMKSGPFARLPAARAPNWTLLAQSVDLHDDATADL
                     LHRFRFISDARLDDAMISIATDGGGVGPHFDSYDVFLLQALGQRRWRISSQKDLTLEP
                     GLPLKILKNFKAEHEYLLEPGDMLYLPPQVAHDGVAVGDCMTISIGFRAPTLSFLAQG
                     LLEQASEQIAAIAHSTADAHGLRSPVGFAPAKTYSDIGSPATATPARLPDLMVNATLD
                     AIKKIRFDDTMAAQFLGLWLTELPPNAYFEPDSGHIDLNSNIPATGALVLARCTRLMY
                     RDKQLFINGEVSPAPALKIMRELADARQLKCTAATAKMLRKREAGILTSWLREGWLHY
                     AQEEQ"
     gene            complement(1488..2606)
                     /gene="bamC"
                     /locus_tag="VIR04_06080"
     CDS             complement(1488..2606)
                     /gene="bamC"
                     /locus_tag="VIR04_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY9460350.1"
                     /translation="MNKRLAGLTIGLGFLVLSGCSQMNQLMGREEAVDYKSTVAGDPL
                     SIPPDLTQANNDVRYKAPAGTATYSQYQQEQQDRKSSGDADSVLPQTPGIKVMRDGDV
                     RWLDIDKPAEDVYPKIVDFWGQQGFTLHSQDPKAGLMETDWAENRAKIPEGWIRSALG
                     SILDQVFDSGERERFRTRVERVNGRTEVYITHDQMVETPTSPDTSTFKWVAGKEDPGL
                     NAAMLARLVVFLGTDVQKARAMVAQAEKSSGQPQAVKATEGQAELTLAEPFDRAWRRV
                     GVAIDSAGFSVDDRDRSAGDYFIRYLDTDTGQKIDQGNFMTRLFGAKNTAQATPYRIH
                     VGTQGSGTAVTVLDQKGQRINTPTAQRIVAVLSTHMGD"
     gene            complement(2623..3525)
                     /gene="dapA"
                     /locus_tag="VIR04_06085"
     CDS             complement(2623..3525)
                     /gene="dapA"
                     /locus_tag="VIR04_06085"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY9460351.1"
                     /translation="MARTVSDTPVFRGSMVALITPMHPDGTLDFDTYRRLIDWHVEQG
                     TDALVVVGTSGESPTVTIDENSELIRIAVEHAAGRIPVIAGVGANSTAEAIHLSNHAK
                     KVGAQAGLSVVPYYNKPTQEGSYQHFRAIAEASGLPLILYNVPGRTSSDITNETILRL
                     AQVPGIIGVKDATGNISRVASLVHDAPPEFQLFSGDDATAAALILLGGRGNISVTANV
                     APKLMHELCHAALEGDVARTRQLNGLLAHLNKMLFIESNPIPVKWAVAEMGLSELGYR
                     LPLTPLHEQYRSAVRASLKDVGLI"
     gene            complement(3608..4258)
                     /locus_tag="VIR04_06090"
     CDS             complement(3608..4258)
                     /locus_tag="VIR04_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY9460352.1"
                     /translation="MDSLIQTIAVYALPVLFGITLHEAAHGYVARIFGDPTAYEAGRI
                     SLNPVRHIDLVGTIVVPLVLLFSTKLLGGAGLLFGWAKPVPVDWGRLRRPKRDMLWVA
                     LAGPASNIIMAIIWAIALRFLADAGAVQDNFWVQMAIAGIQVNLILMALNLVPVPPLD
                     GGRIVFSLLPGRMAYKYSRIEPYGLAILIVLMLTGALWVVLQPLLAFGQVIINWFL"
     gene            complement(4333..>4858)
                     /locus_tag="VIR04_06095"
     CDS             complement(4333..>4858)
                     /locus_tag="VIR04_06095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY9460353.1"
                     /translation="DSSYAVLARLDDPAAAEALRRLRGLDNRHHLTLLCRDLTEIGHF
                     ARIDNRQYRLLKMATPGPWTFILDATREVPRRVSHPSRKTIGIRVPSHRVALDLIEFA
                     GSPLMSTTLIPQGEEEPLNDAQLIFERCGNQLAAVIDGGPCPVKPTTVIDLTGPEPEV
                     IRRGQGDLATLGLD"
BASE COUNT         1089 a         1474 c         1320 g          975 t
ORIGIN      
        1 ccaggttgca cagcgcgccg gcattccccc agccgtaatc cgccaggcca gccgcgagct
       61 atcccggctg gaagctcaag gcgctccgac tccgcaactc gacctgttca gttccgtagc
      121 cggcgacttg cctgacgagc ccgccgccaa tgatcctgac cccgcaaccg gccctgcggg
      181 actgaccctc aaggccaggc ttgccgcgat agaacctgat caattaaccc cgcgcgaggc
      241 gctggacatt ctctatcatc tgcgcgccga cttcccatga atatcaatcg acctctcgat
      301 cttctcggcg gcctttcgcc gcaagccttc atgcggcatt actggcaacg caaacccctg
      361 ctggttcgtc aggccatccc cggctttcgg cccaaccttt ccattgccga catcaaacaa
      421 ctggtccggc gcgacgaggt cgaatcccgt ctgatccggc gcgacgggaa cggctggaac
      481 atgaagtcag gcccttttgc ccgcttgccc gcagcccgcg cgccaaactg gacactgctg
      541 gcgcaaagcg tggatctgca cgacgacgcg accgccgatc tgttgcaccg gtttcgcttt
      601 atttcggacg cccggctgga tgatgcaatg atcagcatcg cgaccgacgg cggcggcgtg
      661 ggtcctcact tcgacagcta cgatgttttc ctgttgcaag cgctgggtca gcggcgttgg
      721 cgcatcagtt cccaaaaaga cctgacgctc gaacccggtt taccgctcaa gattttgaag
      781 aacttcaagg cagagcatga atacttgctt gagcccggag atatgctgta tttaccgccg
      841 caagtggcgc acgatggcgt agcggtgggc gactgcatga ccatctcgat tggatttcgc
      901 gcgcccactt tgtcctttct ggctcagggt ctgctcgaac aagccagcga gcaaattgca
      961 gcgattgcac acagcacggc ggacgctcat ggtctgcgct cgccagtcgg cttcgcgccg
     1021 gccaagacct acagcgatat cggcagcccc gcgacagcga cgcccgcgcg tttgcctgac
     1081 ttaatggtga atgccacgct tgatgcaata aaaaaaatcc gtttcgacga caccatggcg
     1141 gcgcaatttc tgggattatg gctaaccgag ctgccgccca acgcctactt tgagcccgat
     1201 agcgggcaca tcgacctgaa cagcaacata ccggccacag gcgcactggt gctggcccgc
     1261 tgcacccgcc ttatgtatcg cgacaagcag ctttttatca atggtgaagt cagccccgcc
     1321 cccgcattga aaatcatgcg agagctggcc gacgcacgac aattaaaatg cacggccgct
     1381 accgcgaaaa tgctgcgcaa gcgcgaagcc ggcattctga caagctggct acgcgaaggc
     1441 tggctgcact acgcgcaaga agaacagtaa tcgccgaacc ggctacgcta atcgcccatg
     1501 tgagttgaca gcaccgccac aatacgctga gcggtgggag tattgatgcg ctgccctttc
     1561 tgatccagca ctgtcaccgc cgtcccggaa ccctgcgttc caacgtgaat gcgatacggc
     1621 gttgcttgag cggtgttttt ggctccaaac aagcgggtca tgaaattgcc ctggtcaatt
     1681 ttctgaccgg tgtccgtatc cagatagcgg ataaagtaat cgccggcgga acgatcgcgg
     1741 tcatcgaccg aaaatccggc cgaatcaatc gccacgccaa cacgccgcca cgccctgtcg
     1801 aacggctcgg cgagcgtcaa ctcggcctgg ccctcggttg cctttacggc ctgaggctga
     1861 ccgctggatt tttcggcctg ggccaccatt gcgcgcgcct tctggacatc ggtgcccaaa
     1921 aacaccacca ggcgagccag catggcggcg ttcagacccg gatcctcttt gccggcgacc
     1981 catttgaagg ttgaagtgtc gggcgaagtc ggggtttcga ccatttgatc gtgcgttatg
     2041 tagacctcgg ttctgccgtt gacccgttcg actcgagtgc gaaaacgctc gcgctcgccg
     2101 ctatcgaaca cttgatccag aatgctgccc aaagcgctgc gtatccagcc ttcggggatt
     2161 tttgcgcggt tctcggccca gtcagtttcc atcaaaccgg ccttgggatc ttgcgaatga
     2221 agcgtaaaac cttgctggcc ccagaaatcg acaatcttgg ggtatacgtc ttcggctggc
     2281 ttgtcgatat caagccagcg cacatcgcca tcgcgcatta ccttgatgcc aggcgtttgc
     2341 ggcagcacgg aatcggcatc gccgctgctt ttgcgatctt gctgttcttg ctgatattga
     2401 ctgtaggtgg ccgtacccgc cggggctttg tagcgcacgt cgttattggc ctgagtcaaa
     2461 tcgggaggga tgcttaaggg atcgcccgca accgtgcttt tataatcaac ggcttcttcg
     2521 cgccccatga gctgattcat ttgcgagcag cccgacagca ccagaaaacc aaggccgatt
     2581 gtcaaaccgg ctaagcgttt gttcatacgc accttcatca ttttaaatta agcccacgtc
     2641 ttttaatgaa gcgcgcaccg cggaacgata ctgctcatgc aaaggagtta gcggcaagcg
     2701 atagcccaat tcagacaggc ccatttcggc cacggcccac ttgaccggaa ttgggttgga
     2761 ctcaataaac aacattttgt tcagatgcgc cagcagacca ttgagctggc gggtgcgcgc
     2821 cacgtcgcct tccagggccg cgtgacataa ctcgtgcatc agcttgggcg cgacgttggc
     2881 cgtcactgaa atattgccgc gcccgcccag cagaatcaat gcagccgccg tggcgtcgtc
     2941 gccgctgaat aattgaaatt cgggcggtgc gtcatgcacc agggaagcga cgcgcgaaat
     3001 attgcctgtt gcgtctttaa cgccaattat tcctggcacc tgcgccaacc gcaggatggt
     3061 ctcgttcgtg atgtcggaac tggttcgccc gggaacgtta tacaggatca gcggcaaccc
     3121 cgacgcttcg gcgatggccc gaaaatgctg gtacgagcct tcctgggtcg gcttgttgta
     3181 atagggaaca accgacaatc cggcctgtgc accaaccttt ttggcatgat tggaaagatg
     3241 aatggcttct gctgttgagt tggcgccaac ccccgcgata acaggaatcc ggcccgccgc
     3301 gtgttcgacc gcaatgcgga ttaattcgga attctcgtct atcgtcacag tcggcgactc
     3361 gcccgacgtg cccaccacca ctagtgcgtc ggtaccctgc tccacgtgcc agtcgatcag
     3421 gcgacggtag gtatcaaaat ccagcgtgcc gtcaggatgc atgggggtta tgagagcaac
     3481 catgctgccc cgaaatacgg gagtgtcaga aaccgtgcgt gccataatag gaaatatctc
     3541 ctcgatcccg ctcaaaaagt aaacctgctc taaccaacct gcaagtttac tggataagcg
     3601 ctgccattta taaaaaccag ttaataatca cctgaccgaa cgccagcaag ggctgtaaaa
     3661 ccacccatag agcgcccgtc agcatcaaaa cgataagaat cgccagaccg taaggctcga
     3721 ttcgggaata tttatacgcc atgcggcccg gcaacaggct gaatacgata cggccgccat
     3781 ccagcggcgg caccggcaca agatttaatg ccatcaaaat caaatttacc tgaatgccgg
     3841 caatcgccat ttgcacccaa aagttatcct gaaccgcgcc cgcatcggcc aagaagcgaa
     3901 gtgcgatggc ccagataata gccatgatta tattagatgc gggacccgca agggccaccc
     3961 acaacatgtc gcgttttggg cggcgcaaac gcccccaatc gacgggcacc ggtttggccc
     4021 acccgaataa cagcccggcg ccgcccagca gtttcgtgct aaagagcaag accagcggaa
     4081 cgacaatcgt gccgacaaga tcgatgtgac gaaccggatt caagctaatg cgcccggcct
     4141 cgtaggctgt gggatcgcca aaaatgcgcg caacgtaacc gtgtgccgcc tcgtgcaggg
     4201 taatgccaaa caacacgggc aatgcgtaca ccgcaatggt ttgaattaat gaatccatga
     4261 ggttccgggg acaatccgcg caaacaattt gcgggagttg gacattaggc cgccaccaag
     4321 cgacaaaatt cgttagtcta accccaaagt ggccaaatcg ccctgcccac gtcgaatcac
     4381 ttcgggctcg ggcccggtca aatcaattac cgtcgtaggt ttcacaggac acgggccccc
     4441 atcaattacg gcggcaagct gattgccgca gcgttcaaaa atcagttgag catcgttaag
     4501 cggttcttct tcgccttgcg gaataagcgt cgtcgacatg agcggcgatc ctgcaaattc
     4561 gatcagatca agcgctaccc gatggctggg gacacgaatg ccaatagttt tgcgcgacgg
     4621 gtgcgacacg cgcctgggca cttcgcgggt agcgtcgaga ataaacgtcc agggcccagg
     4681 cgtagccatt ttcaaaagac ggtactggcg attatcgata cgcgcgaaat gccctatttc
     4741 agtcagatcg cggcacagca gggtaaggtg atggcgattg tccaagccgc gcagacggcg
     4801 tagcgcctcg gccgctgccg ggtcgtcgag ccgggccagc acggcataac tggaatcg
//
LOCUS       DATCXU010000271         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_830924,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000271 DATCXU010000000
VERSION     DATCXU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830924"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIR04_06100"
     CDS             <1..339
                     /locus_tag="VIR04_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TilS substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460354.1"
                     /translation="QHNLARHARQSAQVAQVLDEVCASDFAGLDPSEDGRSFSLAAWR
                     ELSPARQALVLRHWLALFDQPMPSDARLQDAMRQLRGLHAMGHDRQMRVRHGAAWIRC
                     VKGRVILDQG"
     gene            421..1683
                     /locus_tag="VIR04_06105"
     CDS             421..1683
                     /locus_tag="VIR04_06105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY9460355.1"
                     /translation="MSLIVHKYGGTSMGSVERIQNVARRVAKWHAAGHQVVVVPSAMS
                     GETNRLLGLAKEISAQPDGRELDMLAATGEQASSALLAMALLSQGVAARSYAGWQVPV
                     VTDSSFTRARIQSIDDARICAELDSGRVVIVTGFQGIDEEGNVTTLGRGGSDTSAVAV
                     ATAIKADECLIFTDVDGVYTTDPRVVPEARRMTVVSFEEMLELASLGSKVLQIRSVEF
                     AGKYHMPVRVLSSLTDPLIPIEEEMTSGTLITFEEDQKMEAAVVSGIAFSRNEAKVSL
                     LAVPDVPGVAYAILGPVAAANIDVDMILQNQSVEGTTDFSFTVNRNDFARTLELLKSQ
                     IGPAVGAQEVVSDEKVAKVSIVGIGMRSHVGIASLMFRTMAEEGINIQMISTSEIKTS
                     IIINDKYMELAVRALHKAFGLDQDKAQA"
     gene            1724..1816
                     /locus_tag="VIR04_06110"
     tRNA            1724..1816
                     /locus_tag="VIR04_06110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1758..1760,aa:Ser,seq:gct)
     gene            complement(1944..>2237)
                     /locus_tag="VIR04_06115"
     CDS             complement(1944..>2237)
                     /locus_tag="VIR04_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY9460356.1"
                     /translation="NAAPLTKPDFIGKWMSQIKPVAKRIGPPTGDRKSWIQRLEWGVI
                     EYSLENLMTFPAVREAVDNGVLTLHGAYFGIATGLLFIRDPVTGQFAPFTEAR"
BASE COUNT          471 a          600 c          677 g          489 t
ORIGIN      
        1 cagcataatc tggcgcgcca cgcccgacag agcgctcagg tcgcgcaggt gctcgacgaa
       61 gtttgtgcaa gcgattttgc cgggctcgat ccgtccgagg acgggcgcag tttttcgctt
      121 gccgcgtggc gcgaattatc gcctgcccgg caagcgctgg tattgcgtca ctggctggcg
      181 ctgtttgacc agcccatgcc cagcgacgcc cgactgcaag atgcaatgcg tcaattgcga
      241 gggttgcacg caatggggca cgaccgccag atgcgggtca ggcacggcgc ggcatggata
      301 cgctgcgtca aaggcagagt aatcctggat caggggtaaa accggcttgt tgaaagacgg
      361 taaaatgatc aactttgtct gtcttgcgaa agacggcgtt tcctaattgc agagtgaaag
      421 atgtctctga ttgttcataa atacggcggc acgtcgatgg gctcggtcga gcgcattcag
      481 aatgtggcgc gccgcgtggc caaatggcat gcggccggtc accaggtggt cgtcgtgcca
      541 tccgcaatgt cgggcgaaac caaccgcctt ttgggcctgg ccaaagaaat ttccgctcag
      601 cccgacgggc gcgagctgga catgcttgcc gccacgggcg aacaggccag cagcgccctg
      661 ttggccatgg cgcttttgtc gcagggggtg gctgcgcgca gctacgcggg ttggcaagtg
      721 ccggttgtta ccgactcctc ttttacccgg gcgcgcattc agtccattga cgacgctcgc
      781 atatgcgcgg aacttgattc gggccgtgtc gtgatcgtga ccggttttca gggcatagac
      841 gaggaaggca atgtcaccac gcttgggcgc ggcggctccg atacatccgc tgtcgcagtg
      901 gctacggcga taaaggccga tgaatgcttg atctttaccg acgtcgacgg cgtttataca
      961 accgacccgc gggtggttcc cgaggcgcgc cgcatgacgg tggtgtcgtt cgaagaaatg
     1021 cttgaattgg cgtcgcttgg ttccaaggta ctgcaaatac ggtcggtaga attcgccggc
     1081 aaatatcaca tgcctgtgcg tgttctttca tcgctgaccg atccgctcat accgatcgaa
     1141 gaagaaatga cgtcgggcac tttaattact ttcgaggaag accagaaaat ggaagctgcc
     1201 gtcgtatcag gcatcgcctt tagccggaac gaagctaagg taagcctgct tgccgttccc
     1261 gacgttcccg gcgtcgccta cgcgattctg gggcctgtcg ccgccgcgaa tattgatgtc
     1321 gacatgatct tgcagaatca gtcggttgaa ggcaccaccg atttctcgtt caccgtcaat
     1381 cgcaacgatt tcgcgcgtac gctcgagctg ttgaaatccc aaatcgggcc agcagtgggc
     1441 gcacaagagg tcgtgtccga cgagaaggtg gctaaagttt ccattgtcgg catcggcatg
     1501 cgcagccacg ttggcattgc cagcctgatg tttcgcacca tggcggaaga gggcatcaat
     1561 atccagatga tcagcaccag cgaaatcaag acttccatca ttattaacga caaatacatg
     1621 gaactggctg tgcgcgcctt gcacaaggcg ttcgggctgg atcaggacaa ggcacaggca
     1681 tagtggccaa tttttttctc atgctagaat agcggcctaa ttcggagaca tggccgagtg
     1741 gttgaaggta ctcccctgct aagggagcat gcggcttata cccgcatcga gggttcgaat
     1801 ccctctgtct ccgccagtaa gaaatttttt gctgttcgga atagttcgta gaagttcaga
     1861 aaaaaggcca aagacccgca tgtttactga catggcgggt ttttttgcgc tgcccgatgg
     1921 gtggggcatg ttgatgaatg aggttagcgg gcttcggtaa aaggcgcaaa ctgccccgtg
     1981 accgggtccc ggataaagag caggccggtg gcgatcccga agtaagcgcc atgcagcgtg
     2041 aggacgccat tgtcgacagc ctcgcgaacg gcgggaaagg tcatcagatt ttcaagactg
     2101 tactcgatga cgccccactc aaggcgctgt atccagcttt tccggtcgcc agttggcggc
     2161 cctatgcgct ttgccactgg cttgatttgc gacatccact tgccgatgaa atcaggcttt
     2221 gtcagcgggg cggcgtt
//
LOCUS       DATCXU010000272         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_630945,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000272 DATCXU010000000
VERSION     DATCXU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630945"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VIR04_06120"
     CDS             complement(<1..627)
                     /locus_tag="VIR04_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY9460357.1"
                     /translation="MTDYLLLIVAGFFAGGLNTIAGGGSFLTFPALVYAGVPIVSANA
                     TSAVAVFPGYIGAVAGFRGEIASFKRPTLIKLTIVSLLGGLIGSLLLLITSNETFKVL
                     VPWLLLFATLLFGAGDYLVAWSKRRNFHLGKFLTPGVLCISIYGGYFNGGLGIMLMAL
                     FAVIGMRDMNIMNGLKNGISVVLSAISVITFAWAGLVQWPEAVIMMVAA"
     gene            complement(635..1339)
                     /locus_tag="VIR04_06125"
     CDS             complement(635..1339)
                     /locus_tag="VIR04_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460358.1"
                     /translation="MSLLEVDKINSYYGDSHILFDVSMHVEENEVVALLGRNGAGKST
                     TLKSLMGVVQPKSGVIRHNGVEVQHFAPHQLADRGIQLVPEERRVFGQISVHENLQLA
                     AMTAENPLSLDAIYETFPRLAERKRHRGKQLSGGEQQMVAIARAMIRNAGIIMLDEPF
                     EGLAPLIVRDLVQVCRKLADKGHTVIIVEQNVRAALSLAHRCYIINNGHMVFEGTPTE
                     LQQDRGTIEKYLGVKV"
     gene            complement(1326..>2123)
                     /locus_tag="VIR04_06130"
     CDS             complement(1326..>2123)
                     /locus_tag="VIR04_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460359.1"
                     /translation="QIVPASTSAPGEPVLEARGITKQYGGLVAVSDVSFSLAEGELRG
                     VIGPNGAGKSTFFAMLAGELDTTEGQVFLRGQEITGIGVTAVCQRGMSKSYQINQLFD
                     SLTVRQNMSIPVLARSRGRFRPDMLRGLHRVKGLADQVNAAIELVGLSTRADTRVSEM
                     AYGDKRRLEIGLALATGANVLLFDEPLAGLGPEERVHVVALLKSIRKGRSMVIVEHDM
                     DAMFELAERITVLYEGHKLAEGTPDEIRTNSAVQAAYLGGMDEHESA"
BASE COUNT          477 a          651 c          570 g          425 t
ORIGIN      
        1 cgcagccacc atcattatta ccgcttcggg ccactgaacc aggcctgccc acgcgaaggt
       61 gataaccgag atagctgaaa gcacgactga aatgccgttt ttcagaccat tcatgatgtt
      121 catgtcgcgc atgccgatca cggcaaaaag cgccatcagc ataataccca ggccgccgtt
      181 gaaatagccg ccgtaaatcg aaatgcacaa caccccgggg gttaaaaact tgccaagatg
      241 aaaattgcgc cgcttgctcc aggcgaccag ataatcgccg gccccgaaca gcagcgtggc
      301 gaaaagcagc aaccacggga ccagaacctt gaacgtttcg ttcgacgtga tcagcaacag
      361 caacgacccg atcaggccgc ccagcaagct gacgatagtg agcttgatga gcgttgggcg
      421 cttaaatgac gcgatctcgc cgcgaaaccc cgccacggct ccgatgtaac ctgggaaaac
      481 cgcaactgcg ctggtcgcat tggccgacac aatgggtacg cccgcgtaaa caagagccgg
      541 aaatgtcagg aaactgccac cgcccgctat tgtattaagg ccgcccgcga aaaagccggc
      601 cacaatcaac aacaaataat cagtcacacc gtctttatac tttgacgccc aggtactttt
      661 ctatggtgcc gcggtcttgc tgcagctctg taggtgtgcc ctcaaaaacc atgtgaccat
      721 tattgatgat gtaacaacgg tgagccagcg acaaagccgc gcgcacgttc tgctcgacaa
      781 taatgaccgt gtgccccttg tcagcgagct tgcggcagac ttggaccaga tcgcgcacaa
      841 tcaacggagc caatccttcg aatggctcgt ccagcataat aatgccggcg ttgcggatca
      901 tcgcgcgcgc gatcgccacc atctgctgtt caccgcccga caactgcttg ccgcgatggc
      961 gcttgcgctc agccagccgc gggaacgttt cgtagatggc gtcaagcgac aacgggttct
     1021 cggccgtcat ggctgccagt tgcaggtttt cgtgaacgga tatttggccg aaaacacgcc
     1081 gctcttcggg caccagttga atgccgcgat ccgccaactg gtgtggcgcg aagtgctgca
     1141 cctcgacccc gttatggcgg ataacgccgc tcttgggttg cactaccccc atcagcgact
     1201 tcagtgttgt gcttttaccc gcgccgttgc ggcccagcag cgccacgact tcattttctt
     1261 cgacgtgcat cgacacgtcg aacagaatat gagaatcccc gtagtagcta ttgattttat
     1321 cgacttcaag cagactcatg ctcgtccatc cccccgagat atgctgcctg tacagcggaa
     1381 ttagtgcgga tttcgtcagg cgtgccttcc gcgagtttat ggccttcgta gagcaccgtg
     1441 atgcgttcgg ccagttcaaa catggcatcc atgtcgtgtt caacaattac catgctgcgc
     1501 cctttgcgta tggacttgag caaagccaca acgtgtacgc gctcttcggg gcccaagccc
     1561 gccagcggct catcaaacag cagcacgttg gcgccggtgg ccagcgccag gccaatttca
     1621 agccggcgct tgtcgccata ggccatttca gatacccggg tgtcggcccg ggtcgacagg
     1681 ccgacgagct caatggccgc attgacctgg tcggccaggc ccttgacacg atgcagaccg
     1741 cgcagcatat cgggccggaa gcggccgcgg ctacgcgcca gcacgggaat actcatgttc
     1801 tggcgcacag taagcgaatc gaatagttga ttgatctgat agcttttgct catgccgcgc
     1861 tggcacacag ccgttacgcc gatgccggtg atctcctggc cgcgcaaaaa cacttggcct
     1921 tcggtggtgt ccagttcacc cgccagcatg gcgaagaagg tggatttccc tgctccgttc
     1981 gggccgatca cgccgcgcag ttcgccttcg gcaagtgaaa acgacacatc gctgacggcc
     2041 acgagaccgc catactgttt ggtaatgccg cgtgcttcga gcacaggctc acccggagcg
     2101 cttgtactgg caggaacaat ttg
//
LOCUS       DATCXU010000273         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1103745,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000273 DATCXU010000000
VERSION     DATCXU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103745"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VIR04_06135"
     CDS             complement(<1..192)
                     /locus_tag="VIR04_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEY9460360.1"
                     /translation="MTPSPLSPIIEPEGDRCVMLRFGSALSADVSLACLAAAAKIEQA
                     RLDGVLDVVPSFTTVAVHYE"
     gene            complement(218..1216)
                     /locus_tag="VIR04_06140"
     CDS             complement(218..1216)
                     /locus_tag="VIR04_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY9460361.1"
                     /translation="MKASMKLIDHGKGGSVDVLQLIDADIPVPGPAQVLIEVSYAGVN
                     RPDILQRSGSYAPPADASPYLGLEVSGIIAAVGAEVSEWRVGDAVCALTPGGGYAQYC
                     IVDASNCLPVPRGLSMEQAAALPENYFTVWTNVFERGRLKSGETILIHGGSSGIGLTA
                     IQLSHEFGARVFTTVGNEQKAQACYQYGADKAVLYHSQDFVDVVSRETQGKGVNLVLD
                     MVGGEYIQRNLKCLALEGRLVQIAFLQGSRIDFNAMPIMLKRLTFTGSTLRPRSSAEK
                     AHIAAQLKAHVWPLLEEGRCLPVVYKTFLLNEVRQAHALMESSAHIGKIVLRVKGA"
     gene            complement(1191..>2075)
                     /locus_tag="VIR04_06145"
     CDS             complement(1191..>2075)
                     /locus_tag="VIR04_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY9460362.1"
                     /translation="DADRPLTLLQVERALQTLSGQHFAFWSCRGRAVNGVYHRGNAPA
                     VFISGAQHANETSGVVGALRAAQQLGDQVDAHFAIVPLENPDGYAMHQALCATHPTHM
                     HHAARYTALGDDLEYRERAPWFERAARDHAFEISRSQLHLNLHGYPAHEWTRPCTGYL
                     PRGFELWSIPKGFFLILRYKSDYKEAALTLLEHVTSKLAEDARLMAYNAVQLQRYKRY
                     SATQPFQVRHGIPYTATQVSNQTPGVTLITEFPDETIYGDEYVFAHTVQTRTVLLASN
                     WWWMNFKGESNESKHEAD"
BASE COUNT          492 a          628 c          529 g          426 t
ORIGIN      
        1 ctcgtagtga acagcaacgg tggtgaacga tgggacgaca tccaaaacgc catctagccg
       61 cgcctgctcg attttggccg cagccgccag acaagccagt gaaacgtccg cacttagcgc
      121 cgatccaaag cgcagcatca cgcaacgatc accttcaggt tcgataatgg gagacagcgg
      181 cgacggtgtc atagtagcgc cattataacg acgccggcta agctcctttc actcgcaaca
      241 ctatttttcc gatatgagcg ctactttcca tcagcgcgtg cgcctgacgg acttcgttca
      301 acaaaaatgt tttataaaca accggcaagc accgcccttc ctcgagcaat ggccaaacat
      361 gagccttcag ttgagccgca atatgcgctt tttccgccga cgatctcgga cgcaaggtgg
      421 atccggtgaa ggtcaggcgc ttgagcatga tgggcatggc gttaaagtca atacggctgc
      481 cctgaagaaa cgctatctgc accagccgtc cctcaagcgc caggcatttc agattgcgct
      541 gaatgtattc gccgccgacc atgtccagca ccaaattgac gcccttgccc tgagtttctc
      601 tggaaacgac gtcaacgaaa tcctgcgaat gataaagcac cgctttatcg gctccgtact
      661 gataacacgc ctgcgctttt tgttcgttgc ccactgtcgt gaaaacgcgc gcgccgaact
      721 cgtgcgataa ctgaatggcg gtcaggccaa tgccgctcga tccaccgtgg atcaggatgg
      781 tctcgcccga tttaagccgg ccgcgctcaa atacattcgt ccagaccgtg aaataatttt
      841 cgggcagcgc ggcggcctgc tccatcgaca agcccctggg gacaggcaga caattcgaag
      901 cgtccacgat gcaatattgc gcatacccgc cgcccggtgt cagggcgcac accgcatcgc
      961 caacgcgcca ctcgctcact tcggctccga cagcggcgat aatgcccgac acctcaaggc
     1021 cgagataagg cgacgcatcg gccggcggcg cataagagcc ggaccgctgc aatatgtcgg
     1081 gacggttaac tcccgcgtaa ctcacttcaa tcaatacctg cgcagggcct ggaacaggaa
     1141 tgtccgcatc aatcagttga agtacgtcaa cgctccctcc cttgccgtga tcaatcagct
     1201 tcatgcttgc tttcattgct ctcgccttta aaattcatcc accaccaatt gctggccagc
     1261 agtacggtgc gcgtctgcac cgtatgcgca aatacatatt cgtcgccgta aatggtttcg
     1321 tcgggaaact cggttattaa agtaacgccc ggggtttgat tggaaacctg agtcgccgta
     1381 tagggaatgc catgcctgac ctgaaacggc tgcgtggcgc tataacgctt gtaacgctgc
     1441 agttgaacgg cgttataagc catcaaacgc gcgtcttcgg ccagcttgct cgtcacatgt
     1501 tccagcaaag tcaatgctgc ttctttatag tcggacttat aacgcaaaat caagaagaag
     1561 cctttcggaa tactccacaa ttcaaatccg cgcggcaaat aaccggtgca tggccgcgtc
     1621 cattcatgtg ccggatagcc atgcaaattc aaatgcaact gagatcggct aatttcgaac
     1681 gcatggtcgc gcgcagcgcg ctcaaaccac ggcgctcgct cgcgatactc aagatcgtcg
     1741 cccaaggctg tatagcgggc cgcatggtgc atatgcgttg gatgcgttgc gcacaatgcc
     1801 tgatgcatgg cataaccgtc gggattttca agcggcacaa tggcaaaatg ggcatcgacc
     1861 tgatcaccaa gctgttgcgc tgcccgcaag gcgcccacca cccctgatgt ctcgttggca
     1921 tgctgcgcgc cactaataaa aacggcgggc gcgttgcccc ggtgatagac cccgttaacc
     1981 gcgcgccccc ggcaagacca aaacgcaaaa tgctggcccg aaagcgtttg caacgccctt
     2041 tcaacctgca acaaggtaag cggccggtcg gcgtc
//
LOCUS       DATCXU010000274         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1026645,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000274 DATCXU010000000
VERSION     DATCXU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026645"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..702
                     /locus_tag="VIR04_06150"
     CDS             115..702
                     /locus_tag="VIR04_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460363.1"
                     /translation="MDIFYSGTTRGFYRRDIHGESIPADAVEITREKHAALLQAQSDG
                     KTIEPDENGFPIAVEPPALTAAQIWEGIKAERDRRSQQSGFQCVGKWFHSDSISKTQH
                     LGNKDTARDQLDEGGAMDDALVDPATGENIVWKTMDGTWQPLTCQIAFDIVKAGKGAE
                     FAHYRAAEMHRVAMEASAQPAEYDYSTGWPLAFGE"
     gene            705..1139
                     /locus_tag="VIR04_06155"
     CDS             705..1139
                     /locus_tag="VIR04_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460364.1"
                     /translation="MQVAFYKGRSRIFNRLVAWWTRGAFSHCELVTGYTSDGAAICWS
                     SSWLDGGVRRKAIKLNPDHWELVDIPDTPRTRTGAVTWFMVNSGQPYDLLGLLGFLWR
                     PTRDVLHGWFCSEAIAAALGIPEPWRFDPNTLRAVLLAHIGK"
     gene            1141..1767
                     /locus_tag="VIR04_06160"
     CDS             1141..1767
                     /locus_tag="VIR04_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460365.1"
                     /translation="MFKHYCAAAWQFIAHGLVTVPLLLLGMPLVAIGLLFRRTVPGTL
                     RPFTQHAPGDWELVRLPRLLLWWDNTVDGMLGDKRGSWNKQTGDCRRFLSMWRWAAIR
                     NPANYFKRFILTCDVRACRIVKLLGQDYVRDDFNSTGAHLLLADNGRRRFYHLYFVRR
                     WGKSKRAIVVQLGYKFRLGDESVIYSYENARNAFKGFTFEANPAKDIS"
     gene            1917..>2836
                     /locus_tag="VIR04_06165"
     CDS             1917..>2836
                     /locus_tag="VIR04_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460366.1"
                     /translation="MSEKFLHGVEVLEIDAGPRPISTVRSSVIGIIGTAPDAEAQATA
                     SLATGDEAIDNALTWTASLAGALGNRITVTLNNPRANDAALSVAVSAGTNITANLATG
                     ATGTITTTAAQLMAAIAANEAAAALVTVTATGLSDGTGAVEALRQTALAGGLDDAFPL
                     NTPVMVAGSRTHAAKLGDAGTLPAAMDSIFDQAGAVVIVVRVAEEADPAEQLAAVLGG
                     VNATTGDYEGVHCFLGAESKVGFAPRILLAPGFTHERDSDSANAVVAELVGIAERLRA
                     VILVDGPDTTDDEAIAAAGDFGTARAYLCDP"
BASE COUNT          572 a          807 c          901 g          556 t
ORIGIN      
        1 cccgcaatac acttgtcaag tgcgtgcgat atccgcttca gaaattacaa tccgaaacct
       61 tggcgcttcg actaacacgt tcggctttat tatcatcggc agataggggc caaaatggat
      121 atattttact caggaaccac gcgaggcttc tatcgccgtg acatccacgg cgagagcatt
      181 ccggccgacg cggtggagat tacgcgcgaa aaacatgctg cgctgttgca ggcgcagagc
      241 gatggcaaga ctatagagcc ggacgaaaac ggctttccga tagcggtcga accgccggcg
      301 ctgacggcgg cgcaaatatg ggagggcatc aaagccgagc gcgaccggcg atcacaacaa
      361 agcgggttcc aatgcgtcgg gaaatggttc cactctgaca gcatcagcaa aacgcagcac
      421 cttggaaata aggacacggc gcgcgaccag ctagacgaag gtggcgcgat ggacgacgcg
      481 ctggttgacc ctgcaaccgg ggagaacatc gtctggaaaa cgatggatgg cacatggcag
      541 ccgctaacgt gccagattgc ctttgatatc gtgaaggccg gcaagggcgc agagtttgcg
      601 cattatcgcg ccgcagaaat gcaccgcgtt gcgatggagg cgagcgcgca gccggctgaa
      661 tatgactatt ccaccggctg gccgctggcc tttggcgaat aaaaatgcaa gtggcgttct
      721 acaagggccg cagccggatt tttaaccggc tggtggcctg gtggacgcgc ggcgcgttct
      781 cgcattgcga gctagtgacc gggtacacca gcgatggcgc cgccatttgc tggtccagtt
      841 cctggctaga cggtggcgtg cgacgcaagg caatcaagct gaatccagac cactgggaac
      901 tggtggacat tccagacacg ccccgaacgc gcactggcgc ggtaacgtgg ttcatggtga
      961 acagcggaca accgtatgac ctgctggggc tgctgggttt tttgtggcgg ccaacgcgcg
     1021 atgtattgca cggctggttt tgcagtgaag caattgcggc ggcgctggga atcccggaac
     1081 cgtggcggtt tgatccgaac acacttcgcg cggtattgct ggcgcacatt ggaaaataat
     1141 atgtttaaac actattgcgc tgccgcgtgg cagtttattg cgcacggcct tgttacggtg
     1201 ccgctgctgt tgcttggtat gccgctggtg gccatcggtc tgctgttccg ccgaacggtt
     1261 cctggcacac ttcggccctt cacgcagcac gcgccagggg actgggaact ggtgcgcctg
     1321 cctcgtttgc tgttgtggtg ggataacacg gtggacggca tgctgggcga caagcgcggc
     1381 agttggaaca agcaaacagg cgactgccgg cgcttcctgt caatgtggcg atgggcggcg
     1441 attcgcaacc cagcaaatta ttttaagcgg ttcattctca cttgcgacgt gcgcgcctgt
     1501 cgcatcgtca agctgctggg ccaggactac gtgcgcgacg atttcaacag cactggcgcc
     1561 cacctattgc tggcggataa cggccggcgc cgtttctatc acctgtattt cgtgcgccgc
     1621 tggggcaaat caaagcgcgc gattgtggtg cagcttggct acaaattccg cctgggcgat
     1681 gaatccgtaa tttacagcta tgagaacgcg cgcaatgcgt tcaaaggttt tacgttcgag
     1741 gctaacccgg ccaaggacat tagctaaaaa aattccccct gcctgttttg gccgcctttg
     1801 tgcggccttt ttttcgtcca tcgcgtggaa aacccgcaag aggaatgccg cgcgcgggct
     1861 ggccaaaatt caggctgcaa ataacttttt tccggtacac caagggaccg caagcgatga
     1921 gcgaaaaatt cttacatggc gtcgaagttc tcgaaatcga cgccggcccg cgtccgattt
     1981 ccacggttcg gtccagtgtg attggcatca tcggcaccgc gcccgatgca gaggcgcagg
     2041 ccaccgcatc gcttgcgact ggtgacgagg caattgacaa cgcactgacc tggaccgcca
     2101 gccttgccgg cgcgctgggg aaccgcatta ccgtgacgct gaacaatccg cgcgcaaatg
     2161 atgcggcgct gtctgtcgca gtgagtgctg ggacaaacat cacagcgaac ctggctaccg
     2221 gcgcaacggg caccatcacc accactgccg cgcaactgat ggcggcgatt gcggccaacg
     2281 aagcggccgc cgcgctggtg acggttacgg ccaccgggct gtccgatggc accggcgccg
     2341 tggaagcgtt gcgccaaacg gccttggctg gcggcctgga cgatgctttt ccgctcaata
     2401 cgcctgtgat ggtcgcgggt tcgcgcacgc acgcagcgaa gctgggcgac gctggcacgc
     2461 tgccggcggc tatggattcg attttcgacc aggccggcgc ggtggttatc gtggttcgcg
     2521 tggccgagga agcggacccg gccgagcagt tggccgccgt cctgggcggg gtgaacgcga
     2581 cgaccggcga ttatgaaggc gtccattgtt tcctgggcgc agaaagcaaa gtgggctttg
     2641 cgccgcgcat cctgctggcg cctggtttca cgcatgagcg cgacagcgac agcgccaacg
     2701 cggtggtggc ggaattggta ggtatcgcgg agcgcctgcg cgccgtcatt cttgtggatg
     2761 gcccggacac gaccgacgac gaagctatcg ccgctgcggg cgatttcggc accgcgcgcg
     2821 cttacttgtg tgatcc
//
LOCUS       DATCXU010000275         7255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_880038,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000275 DATCXU010000000
VERSION     DATCXU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880038"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VIR04_06170"
     CDS             <1..1002
                     /locus_tag="VIR04_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY9460367.1"
                     /translation="KAFLHPIAQRPNLTVMTEAQVQQIVFAEHRADGVNFIHEGQKLF
                     ARARSEVILSSGSIGSVQLLQVSGVGPGALLQSLGIPVIHDLPGVGQNLQDHLQLRLI
                     YKVQGAKTLNTMVSSMWGKARMGLEYALRKSGPLAMAPSQLGAFTRSGPEQTRANLEY
                     HVQPLSLEKFGDALHEFPAFTASVSNIRPTSRGHVQIVSPSAQDAPAILCNYLQTEED
                     RRIAADSIRLTRRIVAQPALARYKPEEYKPGAQIDTQADLEHAASEVGTTIFHPVGTC
                     KMGVDPMAVVDPELRVHGLQGLRVIDASIMPTITSGNTNSPTIMIAEKGARFILAAA"
     gene            1092..2414
                     /locus_tag="VIR04_06175"
     CDS             1092..2414
                     /locus_tag="VIR04_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEY9460368.1"
                     /translation="MPNSIEVVATALFVIAIIHTFSVPVFARLSSRGGSHAGLWHLLS
                     EVEVVFGFWAFILLVLMAFLSGSADAVRYIDTRDFTEPAFVFVIMVVAASRPILELVK
                     FLVRGLARMTPVRSELAMFFITLSFVPLSGSLITEPAAMTLAALLLRDSFFSRTGQSG
                     VKYMTIGVLFVNVSIGGVLTSYAAPPVLMVAHTFGWDTLFMATHFGWRAAGAVFFNAA
                     VLTLICRSCLLSGVMESGRGAAAHAANDSTNPAARPGQARPAVPFAIMAVHLIFLVAV
                     VLSAHHPIIFLGLFLLFLGFTSAYSSHQSRLMIKEALMVGFFLAGLVTLGGMQKWWLQ
                     DLLAGLSPVVLFWGATALTAVTDNAALTYLGSLVDGTSELWRYMLVAGSVTGGGLTVI
                     ANAPNPAGFSILKGCFPDGAISPLKLLYAASLPTAVAAGMFLLPNSFG"
     gene            2608..2994
                     /locus_tag="VIR04_06180"
     CDS             2608..2994
                     /locus_tag="VIR04_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY9460369.1"
                     /translation="MPIYVYKCSACGHGEDVLQKMSDPLLTVCPECGHHTYQKQVTAA
                     GFQLKGTGWYVTDFRGNGGKPPESTGTAPHPHHKPASDGDGSAHSGVTDSAKAAAPAK
                     TALPAPKAGKSASATPASAGASSSAS"
     gene            3002..3655
                     /locus_tag="VIR04_06185"
     CDS             3002..3655
                     /locus_tag="VIR04_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY9460370.1"
                     /translation="MRFFKRYFITGLLVWIPLVITFWVIALLINTLDSVVPRFLSSQS
                     LFGFQIPGFQVLLVLVVVLVTGLFAANFIGRAFVDRWEHLLGRIPLVRSIYNSVKQVS
                     DTILAPNGVAFREAVLVQYPRHGAWTIAFLTGTPSGEVADKLGGEYVSVYVPTTPNPT
                     SGFFLMMPRGDIQVLDMSVDAALKYVVSMGVVSPVEHVHNGEALPPTAAKSQPSIEA"
     gene            3681..5471
                     /gene="aspS"
                     /locus_tag="VIR04_06190"
     CDS             3681..5471
                     /gene="aspS"
                     /locus_tag="VIR04_06190"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY9460371.1"
                     /translation="MRTCYTGEVCRDHLGQTVTLFGWVHRRRDHGGVIFIDLRDRAGL
                     AQIVVDPDNVQAFAIAERLRNEFCIKVVGLVRLRPEGTRNSELASGEIEVLCREIEIL
                     NPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLMLRYRVSIEVRKYLDALGFID
                     VETPMLTKSTPEGARDYLVPSRVNPGHFFALPQSPQLFKQMLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDCETSFLDEVQIREIFEGMIRHVFSTVQKVELPNPFPIMTWGE
                     AMRRYGSDKPDLRVKLEFVDVSELMQDVDFKVFSAPANDPVSRVVALRVPGGARLPRS
                     EIDAYTKFVGIYGARGLAYIKVNDAASIPEGLQSPIVKNIHVAALTRLIELTGAQSGD
                     LIFFGADKAKIVNDAIGALRVKIGLSEFGKQNGLVEPGWKPLWVVDFPMFEYSEEEGR
                     FFAAHHPFTSPKDGHEDFLETDPGRALAKAYDMVLNGWEIGGGSIRIHRAEVQSKVFR
                     ALNIGAEEARDKFGFLLDALQYGAPPHGGVAFGLDRLVTLMTGAESIRDVIAFPKTQR
                     VQCLLTQAPSTVDEKQLRELHIRLRNSDVT"
     gene            5498..6358
                     /locus_tag="VIR04_06195"
     CDS             5498..6358
                     /locus_tag="VIR04_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY9460372.1"
                     /translation="MSVVRVISYNIHKGRSALGGRDSLSDLRLGLYGLRPDLLFLQEV
                     QGRNEQHFNLDAQHESLGAALRMNTAYGCNAVRQRSDHGNALLSRYPILSQENQDISD
                     HRLEQRGLLHVKVQIADTTVHCLVVHLGLFAGGRSRQVAALLARIKRLVPVDAPMLIA
                     GDFNDWNNRLAPLFVKQLGLYEVFAVAPQNPLVPVHNLRNSVTRLSNSLRHLSKPLPL
                     AKSVRELGINGMARMTPPPRTFPAAFPWLRLDRIYQRGFAVRKARVLHSAPWKQLSDH
                     APLIAELELP"
     gene            6408..>7255
                     /locus_tag="VIR04_06200"
     CDS             6408..>7255
                     /locus_tag="VIR04_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY9460373.1"
                     /translation="MHRKNLRIAWREGNQIDLLKNGAEFFSTLCQAIDASQTSVHLET
                     YIFSVDETGLRVLDSLQRACERGVKVRVVFDGFGSARGAVEVSRRLDSMMAQHRIYRP
                     WPRGLLALLPGPRHLRRLHRKTCVVDDRFAFVGGLNILDDLVDVPDDGGGPSPRFDFA
                     VLLQGPIVLDVIKAQSALWLRMAWRRRNDWASFYRRLAGWSKRYLHAKEINALDKGQG
                     RRAALMLRDNVRFRRTIENGYLTQIDQAQTEILLANAYFFPGRRVRKALEHAAGRGVR
                     VRLLLQG"
BASE COUNT         1351 a         1984 c         2162 g         1758 t
ORIGIN      
        1 aaggcttttt tgcatcccat tgcgcagcgt cccaatttaa cggtgatgac cgaggctcag
       61 gttcagcaaa tcgtatttgc cgaacaccgg gccgatggcg tcaactttat tcacgaaggt
      121 caaaagctgt ttgcccgcgc gcggtccgaa gtgattctgt cgtcgggttc tatcggctcg
      181 gttcaattgc tgcaggtatc aggggtgggg ccgggggcgc tactgcaatc cttgggaatc
      241 ccggtgatcc atgatctgcc cggtgtaggc cagaacttgc aggatcactt gcaattgcgg
      301 ctgatttaca aggtgcaggg tgccaaaacc cttaacacca tggtttcgtc aatgtggggc
      361 aaggccagga tggggctgga gtatgccctt cgaaaaagcg gccctctggc gatggctcct
      421 tcgcagttgg gggcatttac ccggtccggg cctgaacaaa cgcgggccaa tcttgaatac
      481 catgttcagc ccttgtctct ggaaaaattt ggcgacgcac tgcacgaatt tccggcgttc
      541 accgcctcgg tcagcaatat acgtcccacc agccggggcc atgttcaaat cgtgtcgccg
      601 agcgcccaag acgcgcccgc gattttatgc aattacttgc aaacagaaga agatcgccgg
      661 atcgcggccg acagcatacg tttgacgcgg cggattgttg ctcagccagc cttggccaga
      721 tacaagcctg aagaatacaa acccggcgcg cagatcgata cgcaggcaga tctcgaacat
      781 gcggcgtccg aagtgggcac caccatattt catcctgtgg gcacgtgcaa aatgggcgtc
      841 gacccgatgg cggtggtcga tcccgaattg cgtgtgcatg gcctgcaggg gctgcgcgtg
      901 atcgacgctt cgatcatgcc caccatcaca tcggggaaca ccaactcgcc gaccatcatg
      961 attgccgaaa agggcgctcg cttcattctg gcggccgctt agccgttaat gcggccctgg
     1021 cggaaaatga cgccgagccg ggcttcgtgc gacaattcag cctattttaa ggttttgaaa
     1081 ggttaggcgg catgcccaat tcgattgaag tggtggcgac ggctctattt gtcattgcca
     1141 ttattcacac gttttccgtt ccagtatttg cccgcctttc aagtcggggc ggatcgcacg
     1201 ccggcctttg gcatttgctt tccgaagtcg aagttgtatt cggattctgg gcgtttattt
     1261 tattggtgct tatggctttt ttaagcggga gcgccgacgc cgttcgttat atcgacacgc
     1321 gcgacttcac cgagcccgcc ttcgtgttcg tgattatggt ggttgccgcc agtcgaccca
     1381 tactggaact ggtgaaattt ttggtacgcg gacttgccag aatgacgccg gttcgatccg
     1441 agttggccat gttttttatt accttgtcct tcgtgcccct gtccggatca ctgatcaccg
     1501 aaccagcggc catgaccttg gccgctctgt tgttgcgcga ctcctttttc agccgaaccg
     1561 ggcaatcggg cgttaaatac atgaccatcg gtgtgttgtt cgtcaatgtg tcgattggcg
     1621 gcgttctgac ttcgtatgcg gcaccaccgg tgctgatggt ggcgcatact ttcggttggg
     1681 acacgctctt catggccacc catttcggct ggcgcgcggc cggcgcagta tttttcaatg
     1741 cggcggtttt gacgctgatt tgccggagtt gcctattgag cggcgtcatg gaaagcggcc
     1801 ggggcgccgc ggcgcatgcc gccaacgatt cgaccaaccc cgccgcccgg cccgggcaag
     1861 ccagacccgc cgtcccgttc gctatcatgg cggtgcatct gatcttcctg gtggcggtgg
     1921 tgctgtccgc gcatcatcct attatcttcc tgggtctgtt tttgcttttt ctgggcttca
     1981 cctctgccta ctcaagtcat caaagccgtc tgatgatcaa ggaagctctt atggtggggt
     2041 tttttctggc tggcctggta acgctgggcg gaatgcaaaa gtggtggttg caggaccttc
     2101 tcgcaggcct gtcgccggtc gtccttttct ggggcgcaac ggcgctgacg gccgtgaccg
     2161 ataacgcggc tttgacctat ctggggtccc tggtcgatgg cacgagcgag ttgtggcgct
     2221 acatgctggt ggcgggctcg gtgacgggcg gtggtctgac cgttattgcg aacgcaccga
     2281 atccggcggg gttctcgatt ttaaaaggct gctttcccga tggtgcgatt tcaccgttaa
     2341 agttgttgta tgccgcgtct ttgccgacag cggtggcggc cggcatgttt ttattgccca
     2401 attcgttcgg gtagacgctt tgcgcgcgca cgtcaatata aattattaaa aacggctaaa
     2461 atcacaagtt ggtcttattt gacgagcgcc ccccggcttc tggcggcgca gcgttgtcaa
     2521 agctttgccg cgagtcgcag ttctattagt acgacggtgc ggcttcaagc gggtttttcc
     2581 ttagacgaac tttcttgggg ttttttcgtg cctatttacg tttacaaatg cagcgcctgc
     2641 ggtcatgggg aagacgtgct gcaaaaaatg tcggatccgc ttttgacggt ttgccccgaa
     2701 tgcggtcacc acacctatca aaaacaagtc acagcggccg gctttcagct taaaggaacc
     2761 ggttggtatg tgactgattt tcgcggtaac ggcgggaaac cgccagaaag cacgggcacg
     2821 gcgccacatc ctcatcataa gccggcgtcc gatggcgatg gttcggctca cagcggcgtt
     2881 actgacagcg ctaaagcggc tgcgccggcc aagacggcgc tgcccgcgcc caaagccggc
     2941 aaatcggcca gcgcgacgcc agcgtccgcc ggcgcttctt cgtcggcaag ctaggagccg
     3001 catgcgtttt ttcaagcgat acttcattac cggcctgctg gtctggattc cgctggtcat
     3061 cactttctgg gttatcgcgc tgctgattaa tacgctggac agcgtcgtgc cgcggttttt
     3121 atcgtcgcag tcgttgtttg gtttccagat tcccggattt caggtgctgc tggtgctggt
     3181 cgtcgtcctg gtgaccggcc tgtttgcggc caacttcatc ggccgcgcct ttgtggaccg
     3241 gtgggagcat ctgctggggc gcatcccgct ggtgcgctcc atttacaatt cggtcaaaca
     3301 agtcagcgac acgattttgg cgcctaacgg cgtggcgttt cgcgaggcag tgctggtgca
     3361 gtaccctcgc cacggagcgt ggacgattgc gtttttaacc ggcactccca gcggcgaggt
     3421 ggccgacaag ctggggggcg agtacgttag cgtctatgtt cccacaacgc ccaatccgac
     3481 ttctggcttc tttctcatga tgcctcgcgg cgatatccag gtgctggata tgagcgtgga
     3541 tgcggcgctg aagtacgtcg tgtctatggg cgttgtttcg ccggtcgagc acgttcacaa
     3601 tggcgaggcg ctaccgccca ccgcggcaaa aagccagcct tcaattgaag cctaagcatt
     3661 tttatacgga gttccccttc atgcgtacct gctataccgg cgaggtttgc cgagatcact
     3721 tgggccagac agttactttg tttggttggg ttcatcgccg tcgcgatcat ggaggtgtaa
     3781 tttttatcga ccttcgcgat cgcgcaggtc tggctcagat tgtggtcgac ccggataatg
     3841 ttcaggcctt tgccatcgcg gagcggttgc gcaacgagtt ttgcataaag gtggtggggc
     3901 tggttcgtct gcggcctgaa ggtacgcgca atagcgagct ggcgtccggc gaaatcgagg
     3961 tattgtgccg cgagattgaa attctcaatc catccgtcac gccgccattt cagcttgatg
     4021 atgacaatct ctccgaaacc acgcggctga cgcaccgggt tcttgatttg cgccgcccgc
     4081 aaatgcagca caatttaatg ttgcgctatc gcgtttcgat cgaggtgcgc aagtatctgg
     4141 acgctttggg ttttatcgat gttgaaaccc ccatgctgac caaaagcacc cccgagggcg
     4201 cacgcgatta tctggtcccg tcgcgcgtta atccagggca tttcttcgcg ttgccgcaat
     4261 ctccgcagtt gttcaagcaa atgttgatgg tggcgggttt tgatcgctac tatcagataa
     4321 ccaagtgctt tcgcgacgaa gatttgcgtg cggatcgtca acccgaattc acgcagattg
     4381 attgtgaaac ttcgttcctg gacgaggtgc aaattcgcga aatttttgag ggcatgattc
     4441 gtcatgtgtt cagcacggtg caaaaggtcg aactgcccaa tccattccct atcatgacct
     4501 ggggcgaggc gatgcgccgc tacggatccg ataagcccga tctgcgcgtc aagcttgaat
     4561 ttgtcgatgt ttccgagttg atgcaagatg tcgatttcaa ggtgttctcg gcccccgcca
     4621 atgatccggt cagccgcgtc gtggccttgc gtgtgccggg cggcgcccgc ttgccgcgta
     4681 gcgaaataga tgcctacaca aaatttgtcg gcatttacgg cgctcgcggc ctggcgtata
     4741 tcaaggttaa cgatgcggcg tcgattcccg aaggcctgca gtcgcctatc gttaaaaaca
     4801 tccatgtggc cgcgttgacc cggcttatcg agcttactgg cgcacaaagc ggcgatttga
     4861 tctttttcgg cgccgacaag gccaagatag tcaacgatgc gatcggcgct ttgcgcgtga
     4921 agatcggttt gagcgaattc ggcaagcaaa atggcctcgt cgagcccggt tggaagcctc
     4981 tgtgggtagt cgacttccca atgtttgaat acagcgagga agaaggccgc tttttcgcgg
     5041 cgcaccaccc tttcaccagc cccaaagatg gccacgagga cttcctggag accgaccctg
     5101 gcagggctct tgccaaggcg tatgatatgg tgttgaacgg ttgggagata ggcggcggtt
     5161 caatacgtat tcaccgcgcc gaggtgcaaa gcaaggtatt tcgcgcgctt aacatcggcg
     5221 ccgaagaagc ccgcgacaag tttggcttct tgcttgatgc gctgcagtat ggcgcgcctc
     5281 ctcacggcgg agtggcgttt gggcttgacc ggttggtgac cctgatgacc ggcgccgaat
     5341 caatacgcga cgtgatcgcg tttccgaaaa cccagcgtgt gcaatgtttg ctgactcagg
     5401 cgccctccac ggtcgatgag aagcaattgc gcgaactgca cattcgtttg cgcaatagcg
     5461 atgtgacctg agggcgtatt ggcgcaaagg tgaggccatg tcggttgtgc gcgtcataag
     5521 ttacaacatc cataagggcc gttccgcgct gggcggccgg gattctttgt cggatctgcg
     5581 cttgggtttg tatggtttgc ggcccgattt gctgttcttg caggaagtcc agggccgcaa
     5641 cgagcagcat ttcaatctgg atgcgcagca tgaatccctg ggcgccgcgt tgcgcatgaa
     5701 cacggcctat gggtgcaatg cggtgcgtca gcgcagcgat catggcaacg ccttgctgtc
     5761 gcgctacccc atacttagcc aggaaaatca ggatatctcc gaccatcggc tggagcagcg
     5821 cgggctcttg cacgtcaagg ttcagatcgc agacactacc gtgcattgcc tggtggtgca
     5881 tctgggcttg tttgcgggcg ggcggtcgcg tcaggtggcg gctttgcttg cacggatcaa
     5941 acgtttggtt cccgtcgatg cgcccatgct gattgcgggg gacttcaacg actggaacaa
     6001 tcggttggcg ccgttgtttg tgaagcaatt gggtctgtac gaggtctttg ccgtggcgcc
     6061 gcaaaatccg ctggttccgg tccataactt gcgcaattcc gttacgcggc taagcaatag
     6121 tttgcgccat ttgtccaagc ccttgccgct ggccaaatcg gtgcgcgagc tgggcatcaa
     6181 tggcatggcg cgtatgacgc cgcctccccg aacttttccc gccgcatttc cctggttgcg
     6241 cctggatcgc atttatcagc gcggctttgc ggtgcgcaag gctcgggtat tgcacagcgc
     6301 cccatggaag cagctctcag accatgcacc cttgatcgcc gaacttgaac tgccttagac
     6361 gcatgccatg ccaatcgtaa atggatccgg cgctgacgag agtcatcatg caccgtaaaa
     6421 acttgcggat agcatggcgc gaaggcaacc agatcgatct tttgaaaaac ggcgcggagt
     6481 ttttcagcac cttgtgtcag gccattgacg cgtcccaaac cagcgtccat ctggaaacgt
     6541 atattttcag tgtcgacgaa acgggtttgc gcgtgcttga cagcttgcag cgcgcctgcg
     6601 aacgaggcgt aaaggtgcgc gtggtatttg acggctttgg cagcgcgcgg ggcgcggtgg
     6661 aagtcagccg gcgcctggat tccatgatgg cgcagcatcg aatataccgt ccctggccgc
     6721 gcggcctgtt ggccttgctc ccggggcctc gccacttgcg tcgtctgcat cgcaagacct
     6781 gcgttgtcga tgatcggttt gcgttcgtcg gcggccttaa tattcttgat gatttggtgg
     6841 atgtgcccga cgatggcggt gggcccagcc cccgttttga ttttgcggtg ctgctgcaag
     6901 gccccatcgt actggatgtg atcaaggcgc aaagcgccct gtggttgcgc atggcgtggc
     6961 gccggcgaaa cgattgggcc agtttttacc gccggttggc cggatggagc aagcgttatt
     7021 tgcacgctaa agaaatcaat gctcttgata aaggtcaggg gcggcgcgcc gcgctgatgt
     7081 tgcgtgataa tgttcgcttt cgccgcacca tcgaaaacgg ctacctgaca cagatcgatc
     7141 aggcgcagac cgagattttg ctggccaacg cttatttttt tccggggcgg cgcgtgcgca
     7201 aagctctgga gcacgccgcc gggcgcgggg tgcgtgtccg acttctgctt caagg
//
LOCUS       DATCXU010000276         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_448087,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000276 DATCXU010000000
VERSION     DATCXU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448087"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VIR04_06205"
     CDS             <1..737
                     /locus_tag="VIR04_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460374.1"
                     /translation="SSSNPECKIDSISQSWSVLSGAADATRSANAMRAVDQHLVQRED
                     RLIRLLNPPFDKSDLNPGYIRGYVPGVRENGGQYTHAAIWAIMAFAKLGQHETAWELL
                     DLINPINHGATADNIAVYKVEPYVMAADVYAVSPHTGRGGWTWYTGSSSWMYRLIIES
                     LLGLHVKGDALTLTPSLPAAWPSFTMHYRFKESTYHIVVERISDARKTGMTVVDGVEH
                     TGPTITLNNDGKGHTIQVTLDGGPLH"
     gene            complement(734..1423)
                     /gene="lipB"
                     /locus_tag="VIR04_06210"
     CDS             complement(734..1423)
                     /gene="lipB"
                     /locus_tag="VIR04_06210"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY9460375.1"
                     /translation="MIFHKWWPRPAPYGDVWRAMQSFTDERKPSSPDEIWLVEHAPVY
                     TLGQAGKPEHILDSGAIPVVCCDRGGQVTYHGPGQVVVYCLIDLHRLGIFVKEYVALI
                     EDVTIESLRELGVANACRKPSAPGVYVPMPRAPMPGTELAKIAALGIKVHNGCAYHGL
                     ALNVEMDLSPFLGINPCGYEGLRTIDLATCGVHASVEQAGEVFVGHLDRAIENVRRIH
                     RDRRHKAELSA"
     gene            complement(1423..1698)
                     /locus_tag="VIR04_06215"
     CDS             complement(1423..1698)
                     /locus_tag="VIR04_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEY9460376.1"
                     /translation="MVTISPEDSLIEYPCDFPIKVMGRARPDFAQTLTEVVRQFDPSF
                     NPATVEMRPSKGGKYLGLTFTVHATSRKQLDDLYRALHAHAMVSVVL"
     gene            complement(1706..2569)
                     /locus_tag="VIR04_06220"
     CDS             complement(1706..2569)
                     /locus_tag="VIR04_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY9460377.1"
                     /translation="MIPNVDNDSIVYLNGEYIRLGDAKISVLDRGFIFGDGIYEVVPA
                     YKRKPFRMDAHLARLERSLKAIKIDVGWKRADWEKLVVDMLQRSPLESSMAYIQITRG
                     VAKREHAFPSNITPTVFCMVAPFERPSAELRERGLTAVGVPDLRWLHCEIKSVSLLGN
                     VLAKQQAVEAGVDEVVQFRDGFLSEGASCNVWAVKDGVLLAPLRNNLILEGIRYGVLE
                     GLAKKNGIPFESRPVSQLEVEQADELMMSSATKEVLPIVTYNGKPVGSGRPGPVYAKL
                     RGSYDAEVAAL"
BASE COUNT          634 a          815 c          723 g          529 t
ORIGIN      
        1 ggtcatcatc gaaccccgaa tgcaagatcg actccatctc gcaaagctgg tcggtgctct
       61 cgggcgccgc cgacgccacg cgttcggcca acgccatgcg ggcggtggac cagcatctgg
      121 tgcaacgcga ggatcgcctg atccggttgc tgaatccgcc ctttgacaaa tccgatttaa
      181 acccgggtta tatccgcgga tatgtgcctg gagtgcgtga aaacggcggg caatacacgc
      241 atgccgccat ctgggcgatc atggcgttcg ccaaactggg gcagcacgaa acagcctggg
      301 aattgcttga tctgatcaat ccgatcaatc atggcgcgac ggccgacaac atcgccgtct
      361 ataaagtcga gccctatgtc atggcggccg acgtgtatgc agtgtcgccg cacaccgggc
      421 gaggcggatg gacatggtac acaggctcgt caagctggat gtaccgcctg atcattgaat
      481 cgctgctcgg cctgcacgta aagggcgacg cgctgaccct gaccccctcg cttcccgccg
      541 cgtggccgtc gttcacgatg cactatcgtt ttaaagaatc gacctatcat atcgtggtcg
      601 aacgcataag cgatgcacgc aaaacaggaa tgacagtcgt ggatggggtc gaacataccg
      661 gcccaaccat cacattgaat aacgacggca aagggcacac tatccaggtc acccttgacg
      721 gcggccccct gcactaagcc gaaagctctg ccttgtgacg cctgtcgcga tggatgcgtc
      781 gcacgttctc gatggcgcga tccaggtggc cgacgaatac ttcgcctgcc tgttcaaccg
      841 aagcatgtac cccgcaggta gccagatcga ttgtccgcag gccttcatag ccgcagggat
      901 ttatgcccaa aaatggcgat aaatccattt ctacattcag cgccagtccg tgataggcgc
      961 aaccgttgtg gaccttgatg cccaaagcgg cgattttagc cagttcagtc cccggcattg
     1021 gagcacgcgg catgggcaca taaacgccgg gcgcggaggg tttgcggcac gcgttggcca
     1081 cgcccagttc gcgcaaggat tcaatcgtga catcctcgat caaggcgaca tattccttca
     1141 cgaaaatgcc cagacgatgc aaatcgatca ggcaataaac aacgacctgg cccgggccat
     1201 ggtaggtcac ctgcccgccg cgatcgcaac acactacggg aatcgccccg ctgtcaagaa
     1261 tgtgctcggg cttgcctgcc tgccccagcg tgtaaaccgg cgcatgctca accagccaga
     1321 tttcgtcggg agacgacggc ttgcgttcat cggtgaacga ctgcatggcc cgccagacat
     1381 cgccgtacgg cgcgggacgc ggccaccatt tatgaaaaat cattataaaa cgaccgacac
     1441 catggcatga gcgtgcaggg cccgatacag atcatcgagc tgcttccgtg aagtggcatg
     1501 cactgtaaat gtaaggccca gatacttgcc ccctttactg gggcgcattt caacggtcgc
     1561 cggattaaat gagggatcga actggcgcac cacctcggtc agcgtctgcg caaaatcggg
     1621 ccgcgccctg cccatgactt tgatgggaaa gtcacagggg tactcaatta gagagtcttc
     1681 aggggaaatg gtgaccatgg cttgatcaaa gggcggcgac ctcggcgtcg tagctgccgc
     1741 gcaatttggc atatacgggc cccgggcgcc cgctacccac aggtttgccg ttatacgtca
     1801 caatcggcaa aacttccttg gtagccgaag acatcatcaa ttcatcggcc tgctcgacct
     1861 caagctgtga aaccggacgt gattcaaagg ggatgccatt cttttttgcc aggccctcaa
     1921 ggacgccgta gcgtatgcct tcaagaatca ggttgttgcg caacggcgcc agcaaaacgc
     1981 cgtccttgac ggcccagaca ttgcacgaag cgccttcaga caaaaatccg tcgcgaaatt
     2041 gcacgacctc gtcaacgcct gcttcgaccg cttgttgttt ggccagcaca ttgcccagca
     2101 aggaaacgga tttaatttcg caatgcagcc agcgcagatc aggcacgcca accgccgtca
     2161 agccacgttc gcgcagttcg gcgctgggcc gttcaaacgg cgccaccata caaaacacgg
     2221 tgggcgtaat attggacgga aacgcgtgtt cgcgcttggc cacgccgcgc gtaatctgta
     2281 tatacgccat gctgctttca agcggtgagc gctgcaacat atcgaccacc agtttttccc
     2341 agtcggcgcg cttccagccc acgtcgatct tgatagcctt caggctgcgt tccaggcgcg
     2401 ccaaatgggc gtccattcga aacggcttgc gcttgtaggc cggcacaacc tcgtagatgc
     2461 catcgccaaa gataaaaccg cgatcaagca ccgaaatctt ggcatcgccc aaacggatat
     2521 attcaccgtt caaataaacg atactgtcat tatcaacatt gggaatcatt ggccccgcct
     2581 cgcacacata aattcaaaaa attaaagtta atggaacatt aatcggatct tgtcatagac
     2641 ccgtccgaag aaacccgcct cggccacatc gtcgagcacc gccaggtcgt cgcggcgcaa
     2701 c
//
LOCUS       DATCXU010000277         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_577688,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000277 DATCXU010000000
VERSION     DATCXU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577688"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..157
                     /locus_tag="VIR04_06225"
     tRNA            81..157
                     /locus_tag="VIR04_06225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115..117,aa:Arg,seq:acg)
     gene            complement(275..949)
                     /locus_tag="VIR04_06230"
     CDS             complement(275..949)
                     /locus_tag="VIR04_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY9460378.1"
                     /translation="MFTVEEKADIRTRFLEIETSNVADVLDELGHPDQGLASDFALQT
                     SGGPIAGWAYTIRGQMTPYPLGGDAEKMSACAGLSDGDVSVWSGNGEGICYFGELIAI
                     GMKERGCVGAIADGGLRDIGWLNAQLFPVMACYRTPVQSISRWKVNAWQVPVFLRGAT
                     TKWVAVRPGDFVLGDSDGGIVIPQEIVSTVLDQAEALTKKERAIRKDLKNGMSLADAL
                     KQYGHI"
     gene            complement(965..1768)
                     /locus_tag="VIR04_06235"
     CDS             complement(965..1768)
                     /locus_tag="VIR04_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEY9460379.1"
                     /translation="MKTYRVGIAGYGAIGKSLVKILDQGIDGIHLTAIAVRDPSRPPD
                     LKQCANPPLVTTIEDLEPHCDIVIECAPARLLPAIAEPFLRAGKKVVVLSSGALLRHP
                     ELLDLAKRHGGQILIPSGAIVGLDALIAAAEGTIYSVKMVTRKPVRGLLGAPYLEQNN
                     IDIAKITEPTLIFRGTPREAAIGFPANLNVSVSLALAGIGPDQTTLEIWADPALNRNK
                     HRVEVESDAASFSMEIQNIPSENPKTGRITAQSVVALLRKFSAPVCVGT"
     gene            complement(1829..>2395)
                     /locus_tag="VIR04_06240"
     CDS             complement(1829..>2395)
                     /locus_tag="VIR04_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460380.1"
                     /translation="FKTLGDLVAAAKARPDALNFGSAGIGTSHHMQTALLELRTGIHM
                     THVPYKGGAPAMTDLIGGQIQLLLEPLPTAVPHIKSGAVRALGVTTKERSPLLPDVPT
                     FMQAGIKDYEASTWFGAFVPVGTPPPIVKKLSDALIKTLDDPALKTLLQARGIEPMPM
                     NHQTFAKFVSSENDTWKRVIEQGHLQVR"
BASE COUNT          464 a          651 c          690 g          590 t
ORIGIN      
        1 tttcttggca agccgggcaa attgctatag atagagtggg acaagtaaaa gattatatgg
       61 tatagttttg cctctttaca gcggctgtag ctcagttgga tagagtactt ggctacgaac
      121 caaggggtcg tggggtcgat tcctgccagc cgcaccagtt ttagggcctt ccgaatggaa
      181 ggccctaatg gtttgtgcta ttcaaacgca gttgtcctat tgggtttgca ctattggttt
      241 gtgccattct ttgcgcacag ccaggcgcgg gtcgtcagat gtgtccgtat tgctttagcg
      301 cgtcggccaa tgacattccg tttttcaagt ctttgcggat ggcccgttcc ttttttgtca
      361 gggcctccgc ttgatcgagg acggtcgaaa cgatttcttg cgggatgacg atgccgccgt
      421 cgctatcgcc gagaacgaaa tcccccgggc ggacggcaac ccatttagtg gttgcgccac
      481 gcaagaacac gggcacctgc caggcattga ccttccaccg gctaatggat tgcacggggg
      541 tgcgatagca ggccatgaca gggaacaact gggcattgag ccatcctata tcgcgaagcc
      601 cgccgtcggc tatcgcgccg acgcacccgc gttctttcat gccgatggca atcagttcgc
      661 cgaagtagca aattccttcg ccgttgcccg accataccga tacatcgccg tccgacagtc
      721 ccgcgcaagc gctcatcttt tcggcgtcgc cgcccagcgg atacggggtc atctgtcctc
      781 tgattgtata ggcccatccc gcgattgggc cgcccgacgt ttgcaacgcg aagtcggagg
      841 ccagaccttg atccggatgg ccgagctcat ccagtacgtc ggcgacgttc gaggtctcga
      901 tttcgagaaa tcgggtgcgt atatccgctt tttcttctac ggtaaacatg agatgcaact
      961 cctgtcatgt gcccacacag accggtgcgg agaatttgcg taacagcgcg accacgcttt
     1021 gtgcggtgat gcgcccggtt tttggatttt cggatggaat gttttggatt tccatagaga
     1081 acgaggcggc gtccgattcg acctcgaccc gatgcttgtt cctgttcagg gcgggatccg
     1141 cccaaatttc aagcgttgtc tggtccgggc cgatccccgc cagcgccagg ctgaccgaca
     1201 cgttcaggtt tgcggggaac ccgatggccg cttcgcgcgg cgtgccgcga aaaatcaggg
     1261 tgggctccgt gatttttgcg atgtcgatat tattttgttc cagataaggg gcgccaagca
     1321 atccgcgcac aggtttgcga gtgaccattt ttaccgaata gattgtgcct tcggccgccg
     1381 ctattaatgc gtcgagtcca acgatcgccc ccgacggaat caatatttgc ccgccgtgcc
     1441 gtttagccag atcaagcagt tcagggtgtc ttaacaatgc gccggagctc aacaccacca
     1501 cttttttacc ggcgcgaaga aagggctcgg cgatagctgg cagcaagcgg gccggcgcgc
     1561 actcgatcac gatgtcgcag tggggctcca ggtcttctat ggtcgtgaca aggggcgggt
     1621 tggcgcattg tttcagatcg ggcgggcgcg acggatcgcg tacggcgatg gctgtcaggt
     1681 gtattccatc gatgccctgg tccaggattt tcaccagcga tttgccaata gcgccgtacc
     1741 cagctattcc tacccggtag gttttcattt gccttcttgc tccgatttct ttgcgatttt
     1801 aatcatgcct gtggcgccag tctgcacttt accgtacctg caggtggcct tgctcgataa
     1861 ctcttttcca tgtgtcgttt tcgctggaga caaattttgc gaacgtctga tgattcatgg
     1921 gcatgggttc aattccgcga gcttgcaaaa gcgttttgag cgcaggatcg tccagcgttt
     1981 ttatcagggc gtcgcttaat tttttcacga tgggcggtgg agttcccact ggaacaaatg
     2041 cgccgaacca tgtgctggcc tcgtaatcct tgataccggc ctgcatgaat gtgggaacat
     2101 ccggcaacaa aggcgaacgc tctttggtgg tcacacctaa cgctctgacc gcgcctgact
     2161 tgatgtgggg cacagcggtt ggcaaaggtt ccagcaataa ctggatctga ccgccgatca
     2221 gatcagtcat ggcgggcgcg ccgcctttat agggaacatg ggtcatgtga atgccggtgc
     2281 gcaattcaag cagggcggtt tgcatatgat ggctcgtgcc gatgccggcc gagccaaagt
     2341 tcagggcatc tggcctggcc tttgccgcag cgaccaggtc gcccagagtt ttgaa
//
LOCUS       DATCXU010000278         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_872167,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000278 DATCXU010000000
VERSION     DATCXU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872167"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="purD"
                     /locus_tag="VIR04_06245"
     CDS             complement(<1..702)
                     /gene="purD"
                     /locus_tag="VIR04_06245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY9460381.1"
                     /translation="MKLLVIGGGGREHALAWRLAQSPRIQRIYVAPGNGGTATSSHME
                     NVNLTKIDELVAFAKKENIAYTVVGPEAPLAAGVVDAFRDAGLKIFGPTQAAAQLESS
                     KDYAKAFMTRYRIPTADYKVFTDSDQAKSYIDAQGAPIVVKADGLAAGKGVVVAGTLE
                     QAHQAIDQMLGPDGRFGAAGARVIVEQCLTGEEASFIVMCDGQNVLALATSQDHKRLK
                     DHDQGPNTGGMGAYSP"
     gene            complement(724..1455)
                     /locus_tag="VIR04_06250"
     CDS             complement(724..1455)
                     /locus_tag="VIR04_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY9460382.1"
                     /translation="MSGHSKWANIQHRKGRQDEKRGKLWTKIIREITVAARVGGADPD
                     MNPRLRLAWDKGTAANMPKENIQRAVARGAGDADAENYEEIRYEGYSAGGAAIIIDCM
                     TDNRMRTVAEVRHALSKHGGNLGLEGSVAYMFKHCGQFLFAPGTSEERVMEVAIDAGA
                     EDVQTDDEGIIEVICAPGDYESLKTAFKRAGLVPEVQNVTMKALSETELADDDAEKMQ
                     KLIDVLESLDDVQEVYTTAIFDESD"
     gene            1563..>2160
                     /locus_tag="VIR04_06255"
     CDS             1563..>2160
                     /locus_tag="VIR04_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY9460383.1"
                     /translation="MVPPIVIARNAKTELVLLPQMANRHGCITGATGTGKTVTLQVLA
                     EQFSRMGTSVFMADVKGDLSGISQAGQPSPKLDDRLKKLGLPTPAFGGNPTVLWDVFG
                     EQGHPVRATVSDMGPLLLARMLELNDTQEGVLSLVFKVADDEGQLILDMKDLRAMLES
                     VTDRASALRGRYGNVSPASMGAIARNLLLLESQGADKFF"
BASE COUNT          407 a          608 c          616 g          529 t
ORIGIN      
        1 tggcgagtag gcgcccatcc cgccggtgtt ggggccctga tcgtgatctt tcaagcgctt
       61 gtggtcctgg ctggtggcaa gcgccagcac attctgacca tcgcacatga cgatgaaact
      121 ggcttcttcg ccggtcaggc actgttccac gatgactcgc gcgcctgcag cgccaaaccg
      181 gccatcgggg cccagcatct ggtcaatggc ctggtgggcc tgttcgaggg tgccggcaac
      241 aaccacgccc ttgccagcgg ccaaaccgtc ggccttgacc acgataggcg cgccttgggc
      301 atcgatgtac gatttcgcct gatctgaatc ggtaaaaact ttgtaatcgg ccgtcgggat
      361 gcgatagcgc gtcatgaatg ccttggcgta gtctttggag ctttcgagct gcgcggcggc
      421 ttgcgtcggg ccgaatattt ttaagccggc gtcgcgaaag gcgtccacca cgcccgccgc
      481 cagcggcgcc tcgggaccca caacggtata ggcaatattt tcttttttgg caaacgcgac
      541 gagttcgtca attttcgtca ggttaacgtt ttccatatgg ctggaagtag cagtgccccc
      601 gttgcccggc gccacatata ttctctggat acgaggcgac tgagccaggc gccatgccaa
      661 agcgtgctcg cggccgccgc cgccgattac aagaagtttc atggttttgg agccttcacg
      721 gatttaatcc gactcgtcga aaattgcggt ggtatagact tcttgtacgt cgtccaggct
      781 ttccagcacg tcaatcaatt tttgcatctt ttccgcgtcg tcatcagcaa gctcggtttc
      841 gcttaatgct ttcatggtga cattttgcac ttccgggacg aggccggcgc gtttaaaggc
      901 cgttttaagc gactcgtagt cgcccggagc gcagattact tcaataatgc cttcatcatc
      961 ggtctgaacg tcttccgcac ccgcgtcaat ggcgacttcc atgacccgtt cttccgaagt
     1021 gccgggtgcg aacagaaatt gaccgcagtg cttgaacata taggcgaccg agccttccag
     1081 ccccaaattg ccgccgtgtt tcgacagtgc gtgccgaact tcggcgacgg tgcgcatccg
     1141 gttgtcggtc atgcaatcga taatgatggc cgcgcctccg gcgctatacc cttcgtagcg
     1201 gatttcttcg tagttttccg catcggcatc gcctgctcca cgcgcgactg ctcgctggat
     1261 attttccttg ggcatgttgg ccgcagtgcc tttatcccag gcaagccgca acctggggtt
     1321 catatcgggg tcggccccgc ctacgcgcgc cgccacggtg atttcgcgga tgatttttgt
     1381 ccatagcttg ccgcgttttt cgtcttgacg gcccttacgg tgctggatat ttgcccattt
     1441 actgtgtccg gacatgggat gtcatcgctt tatgaaagaa aagcgcattt taacccgtgc
     1501 gcggacacga tcatcccatt acacttaacc ttttcgattt gccacgcgtc aaagggtaaa
     1561 aaatggttcc tccgatcgtt attgccagga acgccaagac ggaattagtg ctgttgccgc
     1621 aaatggccaa ccggcatgga tgcatcaccg gcgcgacagg tacgggcaag actgttactt
     1681 tgcaggtctt ggctgagcag ttttccagaa tgggaacttc agttttcatg gcggatgtca
     1741 aaggcgattt aagcggaatt tctcaagcgg gccagccaag tcccaagctg gatgaccgct
     1801 tgaaaaagct ggggctgccg acccccgctt ttggcggcaa tccgactgtg ctatgggatg
     1861 tgtttggcga gcaggggcat cccgtccggg cgactgtttc cgatatgggg cctttgctgt
     1921 tggctcgcat gctcgagctg aacgacactc aggagggggt gttgagcctg gtgtttaaag
     1981 tggctgatga cgaaggccag cttatcctcg atatgaagga tttgcgggcg atgctcgaga
     2041 gcgtgaccga ccgcgcatcc gcgcttcgcg gccgctatgg caacgtgtcg ccggcctcta
     2101 tgggcgccat tgcgcgcaat ctgttgctcc ttgagtcgca aggggccgat aaatttttta
//
LOCUS       DATCXU010000279         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1177540,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000279 DATCXU010000000
VERSION     DATCXU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177540"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VIR04_06260"
     CDS             complement(<1..455)
                     /locus_tag="VIR04_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY9460384.1"
                     /translation="MRDPEDFIVHLPIRYEDETQIVAIAALRAGQAGQVEGRVIHCDV
                     QFRPRRQLVALIEDDTGELALRWLNFYPSQKRQLATGNCIRARGEVRNGFSGLEMVHP
                     KVTVAGGDLPDALTPVYPTTQGLAQSTLRKSIDRALLMADLSDTLPAGFG"
     gene            complement(573..959)
                     /locus_tag="VIR04_06265"
     CDS             complement(573..959)
                     /locus_tag="VIR04_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY9460385.1"
                     /translation="MVKQVIHTDAAPAAVGPYSQAVAASSGKLVFLSGQIGLEPDTGE
                     LISENFEGQVRQSFANMEAVVKAAGGTLNDIVKLTLFLTDLSKFASANAIMADVIPQP
                     YPARSTVGVASLPKGAQFEVEAIIVL"
     gene            complement(1050..1826)
                     /locus_tag="VIR04_06270"
     CDS             complement(1050..1826)
                     /locus_tag="VIR04_06270"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY9460386.1"
                     /translation="MAAFNSEQVLSVRHWNDTLFSFTTTRDAALRFDNGHFVMVGLEI
                     NGKPLVRAYSIASANYEENLEFLSIKVPGGPLTSHLQHLQAGDSVLISKKPVGTLVTD
                     DLAPGKRLYLFATGTGLAPFLSIIKDPGIYERFEKVVLLHGVRRVNELAYADFIQNEL
                     PENEYFGDVVKRKLIYYPTVTREPFRHQGRITHLIETGKLFEDTGLPPLNPRTDRAML
                     CGSPNMLNDISHLLDSLGFKVSAAVGSPGDYVIERAFVEK"
BASE COUNT          521 a          595 c          524 g          487 t
ORIGIN      
        1 ccgaatccgg ccggcaaagt atcggacaaa tccgccatca aaagcgcacg atcaatcgat
       61 ttgcgcagcg ttgactgagc caggccctgc gtggtcgggt agacgggcgt aagcgcatcg
      121 ggcaagtcgc cgcctgccac tgtcaccttg ggatgcacca tttccagacc gctaaaacca
      181 ttgcgcacct caccacgagc acgaatgcag ttgcccgtgg caagctgacg tttctggctg
      241 ggatagaagt taagccagcg caacgccagt tcgcctgtat cgtcttcaat caatgccacc
      301 agttgtctcc ggggccggaa ctgcacatcg caatgaataa cccggccctc gacttgacct
      361 gcctgtccgg cgcgcaatgc tgctatggcg acaatttgcg tttcgtcctc gtagcgaatc
      421 ggcaaatgca caataaaatc ctcgggatcc cgcaaaccca aacgcaatag tttgcgctcg
      481 gtaagcgagt tgccggtagc ggcattgcgc gcgcgggaac ttgcgtttga tttgccgcct
      541 ggatcgcgcg aaaccaccgt caaaatgccc gatcagagaa caataatcgc ttcaacttcg
      601 aattgggcgc ctttgggcaa actggccacc ccaacggtcg agcgggccgg atagggctgg
      661 ggaataacat cggccataat ggcgttcgcg ctggcaaact tgcttagatc agtcaaaaac
      721 aaggttagct tgacgatatc gttcagcgtt cctccggcag ccttgaccac cgcttccatg
      781 ttcgcgaacg actgacgcac ctggccctcg aaattttcgg aaatcagttc gccagtgtcc
      841 ggctcaagtc cgatctggcc cgacaaaaag accagtttcc ctgaactggc agcgaccgct
      901 tgtgaatagg gacccaccgc ggctggggcg gcgtctgtat gaataacctg cttgaccata
      961 gatgctctct gtccatttag taaacaaaca actatcagag tgtagccatc tatagctaat
     1021 attaacagcc cttattcaag aattagcatt tatttttcaa caaaggcgcg ttcaataaca
     1081 taatcgcccg gcgaccctac cgctgcggac accttgaaac ccagcgaatc aagcaaatga
     1141 ctaatatcgt tcagcatatt ggggctgccg cacagcatgg cccggtcggt gcgcgggttc
     1201 agcggcggca ggcctgtgtc ctcgaatagc ttgcctgtct cgatcagatg cgtaatgcgg
     1261 ccttgatgac gaaacggttc gcgtgtgacg gtcgggtaat aaatcagctt tcgtttaacg
     1321 acatcgccaa aatattcgtt ttccggcagc tcattttgaa tgaaatccgc atatgccaac
     1381 tcatttacgc ggcgcacgcc atgcaataaa acgacttttt cgaaacgttc gtaaatgccg
     1441 gggtctttga tgatgctcag aaatggggcc aaacccgtgc cggtggcgaa taaatacagg
     1501 cgtttgcccg gcgcaaggtc gtcagtgacc agcgtgccga ccggcttttt gctgatcaac
     1561 accgaatccc ccgcctgcaa gtgttgcaaa tgcgacgtca acggcccgcc gggaacttta
     1621 atgcttaaaa attccaggtt ttcttcgtaa tttgcactgg caatactgta agcgcgcacc
     1681 aacggcttgc cgttaatttc aagccccacc atgacgaagt gtccattgtc gaagcgcaac
     1741 gccgcgtcgc gcgttgttgt gaacgaaaag agggtgtcgt tccagtgtcg cacgcttaaa
     1801 acctgctcag aattaaatgc cgccatcggt atgcatagaa agaacgaggc catcgcctca
     1861 atgtaatgga ttttaactca cccgaccaaa gaggaccgcg gtcaaagggg gcgtcatcgt
     1921 gcatcaaccg catgcacaat gccgatggtc gtgaataaac gacacgcata tcgctttctt
     1981 acaaacaact gaattaatct gatatcctcc tactttcttg tttgtgcata agaatcattc
     2041 ttattcaaaa tacgtttatc tttcttttct tagaccctca ggagaaattc gatgcgttcg
     2101 ttccgcccgc ccctggtcct gctccga
//
LOCUS       DATCXU010000280         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_122495,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000280 DATCXU010000000
VERSION     DATCXU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122495"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIR04_06275"
     CDS             <1..191
                     /locus_tag="VIR04_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HEY9460387.1"
                     /translation="VAKKAPAKKAAAKKSTAKKAAPATKSTAKAIAKARKATTAAPAI
                     KKAINPAASWPFPTGGRP"
     gene            324..887
                     /locus_tag="VIR04_06280"
     CDS             324..887
                     /locus_tag="VIR04_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY9460388.1"
                     /translation="MLSDVLHFIINIIFSLFGIALILRAWMYAIRLHPFNPYSQAIIR
                     VTDWAIQPMRKVVSPGRYADWPSLIACWATSFIYLILVWLLLTGQAPQLNDIPAALLA
                     SVFTVLKWAFNVVLWLTLIQAVLSWINPLAPVMPVLHTLTAPLLDPIRRVMPNMGGID
                     FSPLILLIVAQIAMMILQHFAFSLFAV"
     gene            complement(901..1401)
                     /locus_tag="VIR04_06285"
     CDS             complement(901..1401)
                     /locus_tag="VIR04_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY9460389.1"
                     /translation="MNSLNSAFLTRRTGRILVLAVAIGALSGLAGCAGQSASSSVYSY
                     GQAQREQVVRTGTIESLRNVTIQNDRPTGFGGLAGGAVGGVVGSTVGRGTGNVLATVG
                     GAIIGAVAGNAVENQTGKTAGVEITVRLDNGETRVIAQANDQPLAIGQRVRMISGNGP
                     TRVVPM"
     gene            complement(1398..2351)
                     /locus_tag="VIR04_06290"
     CDS             complement(1398..2351)
                     /locus_tag="VIR04_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY9460390.1"
                     /translation="MSEKVLVCGSVAFDTIAVFEGYFKDQLLPESLHSLSVSFFVPTM
                     RKEYGGCAGNISYNLRLLGAAPIPVGTVGSDAADYLDRLNRMGIDTHLIRVISDMFTP
                     QCFITTDLDNNQIASFHPGAMLRSAENDLSGTQADWAIVAPDSKDGMFAHAQRLHAQG
                     IPFVFDLGQAMPLFSGEDINKMLDLAQILTANDYEATVVAQRTGRSMSQIAQSLRAVI
                     VTRGAKGATLYSEGVETTIDPVKVDNIVDPTGCGDAHRAGLLYGLTRQWDLVDSCRLA
                     NVMGAFKIASRGPQNHRPDKAEISAALKQHYGLNLLEGVLS"
     gene            complement(2361..>2817)
                     /locus_tag="VIR04_06295"
     CDS             complement(2361..>2817)
                     /locus_tag="VIR04_06295"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY9460391.1"
                     /translation="GLVLLLAQAAFVYRNQIASEAPFLRPVLEKACASLHCKVSYRRR
                     IQLISIMNSSLRSNAKTADPQKNPDRMTLQVTLRNNDDGAQEWPVLTLDLVDFSGTVV
                     AKKNLAPNVYLSADALKKPFAGKSEIIATVPIMLNGIKVNGFQLGKFFP"
BASE COUNT          645 a          829 c          704 g          639 t
ORIGIN      
        1 ccgtagccaa aaaagcccct gcaaaaaagg ccgccgcaaa gaagtccacg gcaaaaaaag
       61 ccgcgccggc gactaaatca acagccaagg caatcgcaaa ggcgcgcaag gcgaccaccg
      121 ccgctcccgc cataaaaaag gcgataaacc cggctgcatc ctggccgttc cctacgggtg
      181 gccgccccta agattaaaga cgggatacca cgtaaataca cgtgaacaac caagctgccc
      241 gattccatcg ggcagctttt tttaaggcat tgatttttcg cctatgcgac aatgcagttt
      301 cggaaaattc aagtagccac gctatgctaa gtgacgtcct gcactttatt attaatataa
      361 ttttctcttt gttcgggatc gcactgatac tgcgcgcctg gatgtacgca attcgcctgc
      421 atccattcaa tccgtactcg caggccatca tccgtgtgac cgactgggcg attcaaccca
      481 tgcgtaaagt cgtctcgccc ggccgctacg ccgactggcc cagtctgatt gcgtgctggg
      541 cgacctcgtt catctattta atacttgtat ggttgctgct gaccggccaa gccccgcaac
      601 taaatgatat tcccgcagca cttctcgcca gcgtctttac cgtactcaag tgggccttta
      661 acgtcgtttt gtggctaacg ctaatacagg ccgtcttatc gtggatcaat cctctggccc
      721 ctgtcatgcc ggtgctgcat acgctgactg cgccgctact cgatcccatc aggcgcgtca
      781 tgcccaacat gggcggcatt gatttctcgc cgcttatact gctgatcgtg gctcaaatcg
      841 ccatgatgat tctgcagcat tttgcgttct cgctattcgc tgtctaggcg ggcgcgaacc
      901 ttacatgggc acaacacgcg ttggaccatt gccgctaatc atgcgaacgc gctgaccaat
      961 agccagcggt tgatcgtttg cctgcgctat aacccgtgtt tcgccgttat ccagacgcac
     1021 ggtgatttca acgcccgctg ttttaccggt ctggttttca accgcgtttc cagcaaccgc
     1081 cccgataatg gcccccccaa cagtggccag gacgttgccc gtgccacgcc cgactgtact
     1141 gccgacaacg ccgcctacgg cgcctccggc cagcccgcca aagccggtgg gcctgtcgtt
     1201 ctgtattgtc acattgcgca gtgattcgat tgtgccggtg cgaactacct gttcgcgctg
     1261 cgcctgaccg tagctatata ccgagctcga agcgctttgg ccggcgcaac cggccaaacc
     1321 agacagcgct ccaatcgcaa cggccaaaac caggatgcgg cccgtgcgcc gggtcaaaaa
     1381 tgcactattt agcgaattca tgaaaggact ccttcaagca aatttaaacc ataatgctgc
     1441 ttcagcgctg cgctgatctc ggctttgtcc gggcgatggt tttgcggtcc ccgcgaggcg
     1501 attttaaatg cgcccatcac attcgccaag cggcaggaat caaccaaatc ccattgccgg
     1561 gtcaaaccat ataacaaccc tgcgcgatgc gcatcgccgc aaccggtggg gtcgacaata
     1621 ttgtcgacct ttacgggatc aatggtcgtc tcgacgcctt ccgaataaag cgtggcccct
     1681 tttgccccgc gggtcacgat taccgcgcgc agcgattgcg caatctgcga catgctgcgc
     1741 cccgtccgct gggccactac ggtcgcttcg tagtcgttgg ctgtgagaat ctgcgccagg
     1801 tccaacattt tattaatatc ttcgcctgaa aacagaggca tggcctggcc aagatcaaac
     1861 acaaatggaa tgccctgagc gtgcaagcgc tgcgcatgtg cgaacatgcc gtctttggag
     1921 tcgggcgcca caatcgccca gtcagcctgg gttccgctta aatcgttttc agcggaacgc
     1981 agcatggcgc ccgggtgaaa tgaggcgatc tggttattgt ccaggtctgt cgtgataaag
     2041 cactgaggtg taaacatgtc ggaaatgacg cggatcaggt gcgtgtcaat gcccatcctg
     2101 ttcaagcgat cgagatagtc cgcggcatcc gagccaaccg tgcccaccgg aatgggagcg
     2161 gcgcccagca aacgcaggtt gtacgagatg ttgccggcgc aaccgccata ttccttgcgc
     2221 atggttggca caaagaaaga cacgcttaat gagtgcaggc tttccggtaa aagttgatct
     2281 ttgaaatagc cttcgaatac tgcaatcgta tcgaatgcca cagagccgca caccaaaacc
     2341 ttttcagaca ttttcatcct ttacgggaaa aatttaccaa gttgaaaacc gtttaccttg
     2401 attccgttaa gcataatcgg cacggtcgcg atgatttcac ttttacccgc aaaaggcttt
     2461 ttcaaggcgt cggccgacaa gtaaacattg ggagccaaat tctttttcgc caccactgta
     2521 cccgagaagt cgaccagatc aagcgtcagt acaggccact cctgcgcgcc gtcatcatta
     2581 ttgcgcagtg ttacttgcaa ggtcatccga tccgggtttt tttgcgggtc cgccgtcttt
     2641 gcgtttgagc gcagcgagga attcataatc gatataagtt gaatacgccg cctatagctg
     2701 accttgcaat gcagggaagc acatgccttc tccagaacgg gtcgcaaaaa aggcgcttcg
     2761 ctggcaatct gatttcgata aacgaatgcg gcttgggcca ataacagaac gagcccc
//
LOCUS       DATCXU010000281         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_481983,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000281 DATCXU010000000
VERSION     DATCXU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481983"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIR04_06300"
     CDS             <1..317
                     /locus_tag="VIR04_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY9460392.1"
                     /translation="PTGYGTLLAEGKETRVIDGKQYVFETPIHADFALIKAHRGDRWG
                     NLVYRKTARNFGPIMATAAKCTVAQVAKVVELGALDPEAIVTPGIFVQRVVLQTPDQE
                     SV"
     gene            317..955
                     /locus_tag="VIR04_06305"
     CDS             317..955
                     /locus_tag="VIR04_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY9460393.1"
                     /translation="MKGLTRDRMAMRVARDIPEGAYVNLGIGLPTMVSNYLPSDREIF
                     LHSENGLLGMGPAPAPGEEDEDLINAGKRPVTLLAGGAYFHQADSFAMMRGGHLDICV
                     LGAFQVSVGGDLANWNTGAPDAIPAVGGAMDLAVGAKQVFVMMEHRTKAGISKIVSQC
                     SYPLTGVACVSRIYTDLAVLDVTPGGLAVREILADLSFVELQRLTGVPLMGL"
     gene            975..1895
                     /locus_tag="VIR04_06310"
     CDS             975..1895
                     /locus_tag="VIR04_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY9460394.1"
                     /translation="MKRGEKISVNAPLPLRDGVAASKVFMPQGTWPSLLAFLLDRFKY
                     MPADVLRNRLADGGIVDEAGVAQTLTSPYQPLRWLWYYREVPDETPVPFDLPVLYHDA
                     HLVVVDKPHFLPSIPAGRHLRETALTRLRDQLDLPQLSPIHRLDRDTAGVMLFCVDQA
                     SRGAYQVLFQSRRVNKEYEAVAAVRADLRLPLVRCSRIEAMSPGFMVREVGGSPNSET
                     HIELIERRGDLGHYRLRPRTGRKHQLRLHMSALGIPVCNDRFYPELQPYRDADDFSDP
                     LQLLARSIEFDDPFSGRRRRFESLRQLSAW"
     gene            complement(1903..3234)
                     /gene="hslU"
                     /locus_tag="VIR04_06315"
     CDS             complement(1903..3234)
                     /gene="hslU"
                     /locus_tag="VIR04_06315"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY9460395.1"
                     /translation="MSAATMTPGEIVSELDKNIVGQDRAKRSVAVALRNRWRRQQVPE
                     PLRNEIVPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDTII
                     RDLIEISIKETRDVETRRVKTQAEDAAEDRILDVLVSPPRGANGEPQREENSARQTFR
                     KRLREGKIDNLEIEIEVANVAPQLEIMAPAGMEEMTEQLKGLFAGMGRDKKKLRKMPI
                     KEAFKLLTDEEAARRINDEDLRTHAISNAEQNGIVFLDEIDKIASRQEQGGADVSRQG
                     VQRDLLPLVEGTAVNTKYGVVRTDHILFIASGAFHLSRPSDLIPELQGRFPIRVELDS
                     LTAKDFVRILCDTDSSLTKQYSALMATEDVTLNFTDEGVQRLAELAFDVNERTENIGA
                     RRLYTVMEKLLEDLSFDASASGGNTVTIDPAYVNDKLEEVAGSQDLSRYVL"
BASE COUNT          575 a          887 c         1035 g          785 t
ORIGIN      
        1 cgccgaccgg atacggaacc ctgctggccg aaggcaagga aacccgcgtc attgacggca
       61 aacagtatgt gttcgaaacg ccgatccacg ctgattttgc gttgatcaaa gcacaccgcg
      121 gcgatcgctg gggcaatctg gtgtatcgca agacagcgcg caactttggc cctattatgg
      181 ccaccgccgc gaaatgcacg gtggcgcaag tcgcgaaggt ggtcgagctg ggggcgctgg
      241 atcccgaagc cattgtcacc cccggcattt tcgtgcaacg cgtcgttttg caaacgcctg
      301 atcaggagag tgtctgatga aaggtcttac ccgcgatcgg atggccatgc gcgttgcgcg
      361 cgatattccg gagggtgcgt atgtcaatct gggcattggc ctgcccacca tggtgtcgaa
      421 ttatttgcct tctgatcgcg aaattttttt gcatagcgaa aacgggctgc ttggcatggg
      481 gccggcgccc gcgccgggcg aggaagatga agacctgatc aatgcgggca aacggccggt
      541 gacgctgtta gccggcggcg cctattttca tcaggcggat tcctttgcga tgatgcgcgg
      601 cggccatctg gacatctgcg tgctgggcgc gtttcaggtg tcggttggcg gcgatctggc
      661 caactggaat acgggcgcac ccgatgcgat tccggccgtt ggcggcgcca tggatctggc
      721 cgtgggcgcg aagcaggtgt ttgtgatgat ggagcatcgc acgaaagccg gcatcagcaa
      781 aatcgtttca cagtgcagct atccgctgac cggcgtcgcg tgcgtcagcc gtatttatac
      841 ggacctggca gtgctggatg tgacgcccgg cgggctggcc gtgcgcgaga ttctggctga
      901 tctttctttt gttgaattgc aaaggctgac cggcgttccg ctgatgggtc tttagcgtgc
      961 cacggtgcgt tggcatgaaa cgcggtgaaa aaatcagcgt gaacgcgccc ttgccgctgc
     1021 gcgacggcgt ggccgccagc aaggtcttca tgccgcaggg aacgtggccc agcctgctcg
     1081 ccttcttgct ggatcggttt aaatacatgc ccgccgatgt gttgcgcaat cgattggccg
     1141 atggcggtat tgtcgatgaa gcgggtgtgg cgcagacatt gaccagtccg tatcaacctt
     1201 tgcgctggct ttggtattac cgcgaggttc cggatgaaac cccggtgccc ttcgatttgc
     1261 cggtgctgta ccacgatgcg catttggtgg tggtggacaa gccgcatttt ctgcccagca
     1321 ttccggcggg ccggcatctg cgcgagacgg cgttgacccg gctgcgcgac cagcttgatt
     1381 tgcctcaatt aagtcccatc catcgactgg accgagacac cgcgggggtc atgctgtttt
     1441 gtgttgacca ggccagtcga ggggcgtatc aggtgttgtt ccagtcgcgg cgcgtcaaca
     1501 aggagtacga ggcggttgcc gccgtgcgcg ccgatttgcg tttgccgctg gtgcgttgca
     1561 gccgtatcga ggcaatgtcg ccgggcttta tggttcgtga ggtgggcggc tcgcccaaca
     1621 gcgaaaccca tatcgagcta attgagcggc gcggcgattt gggccattac cgtctgcggc
     1681 cgcgtacggg ccgcaagcac cagttgcgct tgcacatgag cgctttgggc attcccgttt
     1741 gcaatgatcg tttttacccg gaattgcagc cgtatcggga cgctgatgat ttcagcgacc
     1801 ctttgcagtt attggcgcga tcgatcgaat tcgacgatcc ttttagtggc cggcgccggc
     1861 gttttgaaag tttgcgccag ttgtccgcct ggtgaaacgc cgttaaagca cgtagcgcga
     1921 caggtcctgg ctgcccgcga cttcttcaag cttgtcgttg acgtaggccg ggtcaatggt
     1981 gacggtgttt ccgccgcttg ccgaagcgtc gaaggacaga tcctccagaa gcttttccat
     2041 gacggtatac aggcggcgcg cgccaatgtt ttcggtgcgc tcgttaacat cgaaggccag
     2101 ttcggccagg cgctgcacgc cttcgtccgt aaaattaagg gtgacgtctt cggtcgccat
     2161 gagcgcgctg tattgtttgg tcagcgacga atcggtgtcg cataagatgc gcacgaaatc
     2221 ttttgccgtc agtgaatcaa gctcgacgcg tatggggaag cggccctgca gttcgggaat
     2281 caggtccgaa gggcgcgata aatggaaagc gcccgatgca atgaacaaga tatgatcggt
     2341 gcgaaccacg ccgtatttgg tgtttacggc cgtgccttcg accagcggca gcaagtcgcg
     2401 ttgcacgccc tggcgcgata catcggcgcc cccttgttcc tggcgcgatg cgattttgtc
     2461 gatttcgtcc agaaatacga tgccgttttg ttcggcattg gaaatggcgt gcgtgcgcaa
     2521 gtcttcgtcg ttgatgcggc gcgccgcttc ctcgtcggtc agcaacttga aggcttcttt
     2581 gattggcatt tttcgcaatt tctttttatc gcgccccatg ccggcaaaca ggccttttaa
     2641 ctgctcggtc atttcctcca tgccggcagg agccatgatt tccagttgcg gcgcgacgtt
     2701 tgcgacttca atttcaattt cgagattgtc gattttgcct tcgcgcaaac gcttgcgaaa
     2761 tgtctggcgc gctgaatttt cttcgcgctg aggctcgccg ttggcgccgc gcggcggcga
     2821 cacgagcaca tccagtatgc gatcttcggc ggcgtcttcg gcctgtgttt taacgcggcg
     2881 tgtttcgaca tcgcgcgttt ccttgatcga gatttcgatc aggtcccgga taatggtgtc
     2941 gacgtcgcgc ccgacgtagc cgacttctgt gaacttggtt gcttcgacct tgatgaaagg
     3001 tgcgttggcc agcctggcca ggcgacgcgc aatttcggtt ttgcccacac cggtgggccc
     3061 gatcatgagt atgtttttcg gcacgatttc gttgcgcagc ggctcgggca cttgctggcg
     3121 gcgccagcgg ttgcgcagcg caacggcaac ggaacgtttg gcgcgatctt gcccgacaat
     3181 gtttttatcg agctcggaga cgatttcgcc gggggtcatg gtggcagcgg acataatgga
     3241 tgtttggcgc gtttaaagtg tttcgatggt atggctttgg tt
//
LOCUS       DATCXU010000282         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_651045,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000282 DATCXU010000000
VERSION     DATCXU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651045"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VIR04_06320"
     CDS             <1..326
                     /locus_tag="VIR04_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY9460396.1"
                     /translation="AVGRVRASNAWQLVKRELLVTLCVGTTGSFVTAGFAWAISGSLA
                     VAAVMMFAMIGNMLVGATLGVLIPILRDHFGKDPAMGSSVLLTFATDSLGFFLFLGLA
                     TLFLV"
     gene            complement(363..3107)
                     /gene="secA"
                     /locus_tag="VIR04_06325"
     CDS             complement(363..3107)
                     /gene="secA"
                     /locus_tag="VIR04_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY9460397.1"
                     /translation="MVSLIKKLIGSRNDRLLKQYKKQVAQINALEPSMVALTDEQLKS
                     KTDEFRQRFQKGESLDDLLPEAFAVVRETSKRVFGMRHFDVQLIGAISLHDGQISEMR
                     TGEGKTLMATLAVYLNALSGAGVHVVTVNDYLARRDAEWMGRLYNFLGMTVGVVVPQQ
                     ENEEKKAAYAADITYGTNNEYGFDYLRDNMEYRAEDRRQRGLKYAIVDEVDSILIDEA
                     RTPLIISGQAEDNTDLYVRMNAVPPLLTRMASEPKSQEPEPEGDFWVDEKGQQVHLSD
                     AGHEHSEEILARLKILPEGESLYDPRNITLMHHLMVALRAHNLFFRDQQYVVQNDEVV
                     IVDEFTGRLMAGRRWSDGLHQAVEAKEGVKIQNENQTLASITFQNYFRMYDKLAGMTG
                     TADTEAYEFQEIYSLETVIIPTNRPMVRDDQNDQVFKTDKEKYNAILADIKDCYERGQ
                     PVLVGTTSIENSELLAGMLVKAKLPHEVLNAKQHAREAEIVADAGKPHQITIATNMAG
                     RGTDIVLGGRIENRIDVVRADQTLSDEEKAVRIAAIQDEWKPANEAVKAAGGLRIIGT
                     ERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDSLMRIFAGDRVRSIMERLRLPEGEP
                     IEARMVSRSIESAQRKVEARNFDIRKQLLEYDDVANEQRKVLYTQRNEVLESSDVSET
                     MRNLRNAAITEQYRAHIPEGSMEEQWNVPALEAALDADWQVAMPLVEMLEVEPDLTDD
                     DLLDRVVQEATRLYQAKVELVGHENWAPFERAMLLQSIDTNWRGHLSSLDHLRQGIHL
                     RGYAQKDPKQEYKREAFELFSHMLDRVRTEVVKVLMTVKIESPEQVTDSAPANLENVT
                     YHHSDYDAVLSGADPGLEFDPVNGKAPPLGLAVPKVGRNELCPCGSGKKYKHCHGKLA
                     "
     gene            complement(3166..>4028)
                     /locus_tag="VIR04_06330"
     CDS             complement(3166..>4028)
                     /locus_tag="VIR04_06330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY9460398.1"
                     /translation="WAQSYYGRLTSPSVAQEQMVGKRAGHDSAFVRENVNVLASRIGD
                     LQATLVAMNGLSKRVADAAGVSYTDPEIHAGLQQTALSGMDRVFDEHAEPLSAEDLGR
                     RIDRLAPAVARQKDTFAMLDLALAKRTGIEAGLPTITPVRRFSVGSSFGRRRHPVTGR
                     YTMHEGIDFTAPLGARIRAASGGIVRQAGYRRGYGKMVDIDHGNGLVTRYAHASVLEV
                     EPGDLIEKGQAIGRVGSTGRSTGPHLHFEVRMAGRPLDPALFLSGAAPWREQMADASS
                     RVKAILSKVR"
BASE COUNT          832 a         1213 c         1077 g          906 t
ORIGIN      
        1 tggcggtggg ccgcgtccgg gcatccaatg cgtggcaact ggtcaaacgc gaacttcttg
       61 tcacgctgtg cgtaggaacg accggcagct tcgtcaccgc gggtttcgcg tgggcgattt
      121 cggggtccct ggccgttgcc gccgtgatga tgttcgccat gattggcaat atgctggtgg
      181 gcgcaaccct gggagtgctc atccccatct tgcgcgacca tttcggcaag gaccccgcca
      241 tgggctcgtc ggtcttgctg acctttgcca ctgattcgct gggtttcttt cttttcctgg
      301 ggctggcaac gctgttcctg gtgtaaccgc cgccaccgca acgccgcggc agcggttatt
      361 gatcaggcca gctttccgtg gcaatgcttg tatttttttc cgctgccaca agggcacagc
      421 tcgttgcgcc ccacctttgg aacagccaga cccaaaggag gcgccttgcc gttgactgga
      481 tcaaattcca gacccgggtc ggcgccgctc agaacggcat cgtaatctga atgatggtag
      541 gttacattct cgagattggc cggcgcggaa tcagtcacct gctcgggcga ctcgattttg
      601 accgtcatca gcaccttgac gacctcggtg cgaacacgat cgagcatatg ggaaaaaagc
      661 tcgaaggcct cgcgtttgta ctcctgcttc gggtcttttt gggcgtaccc gcgcaaatga
      721 atgccctgac gcaaatgatc cagcgaggac aggtgtccgc gccagttcgt atcgatcgac
      781 tgcagcagca tcgcgcgttc gaacggagcc cagttctcat gcccgaccag ttcgaccttg
      841 gcttggtaca agcgtgttgc ttcttgcaca acacggtcaa gcaaatcatc atcggtcaag
      901 tcaggctcaa cttccagcat ttcgacaagc ggcattgcca cttgccagtc ggcatcaagg
      961 gccgcctcca acgccggcac attccactgc tcttccatgg atccttcggg tatgtgcgcc
     1021 cggtattgtt ccgtaattgc cgcgttgcgc aagttgcgca ttgtttcaga cacatcggac
     1081 gactcgagca cctcgttgcg ctgtgtatag agcaccttgc gctgctcgtt ggccacatcg
     1141 tcgtactcaa gcaattgctt gcgaatgtca aagttgcgcg cttccacctt gcgttgcgcc
     1201 gactcaatgg aacgcgacac catgcgcgcc tcgatcggct cgccctcggg caaacgcagg
     1261 cgctccataa tggatcgtac gcgatcgcca gcgaaaatcc gcatcagcga atcttcgagc
     1321 gacaaataaa agcgtgacga acctggatcg ccctgacggc cagctctgcc gcgcaactga
     1381 ttgtcgatgc gccgcgactc gtgacgctcg gtgccgatga tgcgcaatcc gccggctgct
     1441 ttgaccgcct cgttggcggg cttccattcg tcctggatcg cggcgatgcg aaccgccttc
     1501 tcttcgtcgg acaatgtctg atcggcgcgg accacgtcaa tccggttctc gatgcgcccg
     1561 cccaatacaa tatcagtgcc gcggcctgcc atattggtgg caatcgtgat ctgatggggc
     1621 ttgccggcat cggcgacaat ttcagcttcg cgcgcatgct gcttggcgtt caggacctcg
     1681 tgcggcaact tggcctttac cagcataccg gccagcagct cggaattctc gatgctggtg
     1741 gtgccgacga gcacgggttg cccgcgctcg taacaatctt taatatcagc cagaatagcg
     1801 ttgtattttt ctttgtctgt cttgaaaacc tgatcgttct gatcgtcacg caccatcgga
     1861 cgattggtcg ggataatcac ggtttccagg gaataaattt cctgaaactc gtacgcttcg
     1921 gtatccgccg tgcctgtcat gccggccagc ttgtcgtaca tgcggaaata attctgaaag
     1981 gtgatcgaag ccagtgtctg gttttcgttc tgaatcttga cgccttcttt ggcctctacc
     2041 gcctgatgca ggccatcgga ccaccgtcgt cccgccatta gacggccggt gaattcgtcg
     2101 acaatcacga cctcgtcgtt ttgaaccaca tactgctggt cgcggaaaaa cagattatgc
     2161 gcccgcaacg ccaccatcaa atgatgcatc agggtgatat tgcgcggatc gtataacgat
     2221 tcgccctcag gcaatatttt aaggcgcgcc agaatttctt cggagtgctc gtggccggcg
     2281 tccgacaaat gaacctgctg gcctttttcg tcgacccaaa agtcgccctc gggttcgggt
     2341 tcctgcgatt taggctcgga ggccatacgg gtcaataggg gcggaaccgc attcatgcgc
     2401 acgtaaagat cggtgttgtc ttcagcctgg cccgaaataa tgagcggcgt acgcgcttcg
     2461 tcaatcagaa tcgaatcgac ctcatcgaca atggcgtact tcagaccgcg ctggcggcga
     2521 tcttcggcgc gatattccat gttgtcgcgc aaatagtcga atccgtattc gttgttggtg
     2581 ccgtacgtaa tgtcagccgc ataggccgcc tttttctctt cgttttcctg ctgcggcaca
     2641 acgacaccga cagtcatgcc caaaaagttg tacaagcgcc ccatccattc agcatcgcgg
     2701 cgcgccaaat aatcattaac ggtcaccaca tgcacacccg cgcccgacag cgcgttcagg
     2761 taaacggcca atgtcgccat cagtgttttg ccttcacctg tgcgcatttc agaaatctgg
     2821 ccgtcatgga gcgaaatggc gcctatcaac tggacatcga agtgacgcat gccaaagacg
     2881 cgcttgctgg tttcacgcac cacggcaaaa gcctctggca gcagatcgtc cagcgattcc
     2941 cctttctgaa accgttgacg gaattcgtcc gtcttggatt taagctgctc gtcggtcagc
     3001 gccaccatgc tgggttcgag cgcgttgatt tgcgccacct gttttttata ttgcttaagc
     3061 aacctgtcgt tgcgactacc aataagcttt tttatcagag aaaccattat tacgccgtcg
     3121 acgcggaaaa aaaccgcagg aaaaaatgca gggaaacgag acagtttaac gcaccttaga
     3181 caggatggct ttaacccggc tcgaggcgtc agccatctgt tcgcgccatg gtgcagcccc
     3241 ggacaaaaac aaggctggat ccagcggacg gccagccatg cgcacttcaa aatgcaaatg
     3301 agggcccgtg gaacgcccgg tcgaaccgac tcgcccaatc gcctgtccct tttcgattaa
     3361 atcgcccggt tcgacttcaa gaactgacgc atgcgcataa cgtgtcacca agccgttgcc
     3421 gtggtcgata tcaaccattt tcccataacc cctgcgatac ccggcttgcc tgacgatgcc
     3481 gcccgacgct gcacgaatcc gggctcccag gggagccgtg aagtcaattc cctcgtgcat
     3541 ggtataacgg cccgttaccg gatggcgccg ccggccaaac gacgagccca cggaaaaacg
     3601 acgcactgga gtaatggtgg gtaagcccgc ttcaatcccg gtccgtttgg caagcgccaa
     3661 atcaagcatg gcaaacgtgt ccttttgccg ggcaaccgcc ggcgcaagcc gatcaatccg
     3721 gcgtcccagg tcttcggctg acaacggttc cgcatgctca tcgaagacac gatccatacc
     3781 ggacaaagcc gtttgctgaa ggccggcgtg aatttcgggg tcggtatacg acacgccggc
     3841 ggcatcggcg acccgtttgc tcagaccatt catggcgaca agcgtcgcct gcaaatcacc
     3901 gattctggaa gccagcacat tgacattttc gcgaacgaag gcggaatcat gccctgcccg
     3961 cttgccaacc atctgctctt gcgccacact tggcgacgtc aagcggccgt aataagactg
     4021 tgcccagg
//
LOCUS       DATCXU010000283         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1062099,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000283 DATCXU010000000
VERSION     DATCXU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062099"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIR04_06335"
     CDS             <1..462
                     /locus_tag="VIR04_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY9460399.1"
                     /translation="RDALQPLAPFAGSGSAYVTSGGSVAASAKAGVPFFVNVGSVAEL
                     DTLLANTSRPVMLDFYADWCVSCVEMEKFTFTDANVAQKMSAMLLLRADVTKNGPDDR
                     ALLKRFKLFGPPGTIFFDARGRELTDARVVGFTKAQPFAAVLDRVLQTASK"
     gene            complement(550..1566)
                     /gene="hemB"
                     /locus_tag="VIR04_06340"
     CDS             complement(550..1566)
                     /gene="hemB"
                     /locus_tag="VIR04_06340"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY9460400.1"
                     /translation="MTSFILSPDFPAARPRRNRRNDFSRRLVRENTLTANDLIYPVFV
                     KEGQSILEPVASLPGVVRYSPDTLLKAAEECVALGIPVMALFPVIDSSLKTPDGIEAT
                     NPDGLIPRVVASLKKHFPDLGVLTDVALDPYTSHGQDGVIDENGYVLNEPTVEILTRQ
                     ALVQAEAGVDIVAPSDMMDGRIGAVRTALEAQNHIYTRIMAYSAKYASAFYGPFRDAV
                     GSAKNLGKSDKFSYQMDPANRSEALREVALDIREGADMVMVKPGLPYLDILRDVKDAF
                     GMPTFVYQVSGEYAMIKAAAANGWLDHDKVMMESLIAFKRAGGDGILTYFAIAAARLL
                     KEQA"
     gene            complement(1563..2186)
                     /gene="yihA"
                     /locus_tag="VIR04_06345"
     CDS             complement(1563..2186)
                     /gene="yihA"
                     /locus_tag="VIR04_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY9460401.1"
                     /translation="MSILHRASFTVSAARLDQLPSPTTAEVCFVGRSNSGKSSAINVL
                     TNQRRLAFSSKTPGRTRLINLFGIPDPIDPNASLGFLVDLPGYGHASVARESREDWAD
                     TLGGYLRERSSLAGIVLLIDIRRGVTELDRRLTDWIAPKPIPVLALLTKADKLPYGQR
                     VNAVFAVKKELAGIGALRAIPFSAPQRIGLEEATHHIENWISPKVVP"
     gene            2337..>2807
                     /locus_tag="VIR04_06350"
     CDS             2337..>2807
                     /locus_tag="VIR04_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9460402.1"
                     /translation="MKRVFSRALLAGGLLLASCVASVGYAAETAVPKPDAAKGEQLYV
                     KGDASRGILACVTCHGAAGNSTIAANPNLSAQAHEYLVKQLTDFKSKDGKAAPLRRGE
                     GGANTVMTAFATALTPADMQNIAYYLAQQPLDYKTAGTATNKASMELGQRIWRGG"
BASE COUNT          566 a          773 c          878 g          590 t
ORIGIN      
        1 cgcgacgcgc tgcagccgct ggcgccgttt gccggttcgg gcagtgctta cgtcacttcc
       61 ggcggttcag ttgcggccag cgccaaggcg ggtgtcccgt ttttcgtcaa cgtcgggtcg
      121 gtggccgaac tggacacatt actggcgaac acttccaggc cggtaatgct ggatttttat
      181 gcggactggt gcgtgtcctg cgtagagatg gagaaattca cattcactga tgcgaatgtg
      241 gcgcaaaaga tgtcggcgat gctgctgcta agggccgatg tgaccaaaaa tgggcctgac
      301 gatcgcgcct tgctgaagcg cttcaagctg ttcgggcctc ccggcacgat atttttcgac
      361 gcacgcggcc gcgagctgac ggatgcgcga gtagtgggct ttacgaaggc acagccattc
      421 gccgcagtgc tggaccgggt gttacaaacc gcttcgaagt aatggggacc agcggacgcc
      481 tttccctcca cgcattcccc ccttcgtgca gcgacaatat ggctttaatt ggatagtgca
      541 agcttcgcac tatgcctgtt ctttcaaaag cctggcggcg gcgatggcga agtaggtcag
      601 tatgccgtcg cctcccgcgc gcttgaatgc gatcagggac tccatcatca ccttgtcgtg
      661 atccagccac ccgttggccg cggcggcttt gatcatcgcg tattcgccgc tgacctgata
      721 gacgaaggtc ggcattccga aagcgtcttt tacgtcgcgc aaaatatcca gataaggaag
      781 gcccggcttc accatcacca tgtctgcacc ttcgcgtatg tccagcgcga cttcgcgcaa
      841 cgcttcgctg cggttggccg gatccatctg gtaactgaac ttgtcggact ttcccagatt
      901 cttcgccgac cccaccgcat cgcggaaggg gccgtagaat gcgctggcgt atttggccga
      961 gtacgccatg atccgggtgt aaatgtggtt ctgagcttca agggccgtgc gcacggcgcc
     1021 gatgcggccg tccatcatgt cgctgggagc cacgatgtca acgccggcct cagcctgcac
     1081 aagcgcctga cgcgtcagga tctcgaccgt cggttcgttc agcacatagc cgttttcgtc
     1141 gattacgcca tcttgcccgt ggctggtgta tggatccagc gccacgtcgg tcagcacccc
     1201 caaatcgggg aaatgtttct ttagcgaggc cactacgcgc ggaatgagcc cgtccggatt
     1261 ggtggcctcg attccgtcgg gcgttttaag gctggaatct atgacgggaa acagggccat
     1321 gacggggatg cccagtgcga cacactcttc ggcggctttc agcaacgtgt cgggcgaata
     1381 ccgcacgacg ccgggcaagg acgccactgg ctccagtatt gattgacctt ctttgacaaa
     1441 aacagggtaa atcaggtcgt tggcggtcaa ggtgttctcg cgcacgagac gacgcgaaaa
     1501 gtcgttgcgg cgattgcggc gggggcgtgc cgcgggaaag tcaggggaga gaataaatga
     1561 agtcatggaa ctaccttcgg agaaatccag ttttcaatat ggtgggttgc ttcttccagc
     1621 ccgatacgct ggggagcgga aaaagggatc gcgcgcaatg cgccgatgcc ggcaagctct
     1681 ttcttgaccg cgaagacggc gttgacgcgt tggccatagg gcagcttgtc cgctttggtc
     1741 aacagcgcca gaaccgggat gggcttgggc gcgatccagt ccgtcaggcg gcgatcaagt
     1801 tcggtgactc cgcggcggat gtcaattagt aaaacaatgc cggccaacga ggagcgctcg
     1861 cgcagatagc cgcccaaggt gtcggcccag tcttcgcggg actctctggc gaccgatgcg
     1921 tggccgtaac cgggcaggtc gactaaaaag cccaacgagg cattggggtc gatgggatca
     1981 ggaatgccaa acaaattaat caggcgggtg cggccaggcg ttttgctgga gaaggccagg
     2041 cgccgctggt tggtcaggac gttgatcgct gacgatttac cggaattgga acggccgacg
     2101 aagcagactt cggcggtcgt tggtgatgga agctggtcaa gccgggccgc cgaaacggtg
     2161 aatgaagcgc gatgcaggat ggacacggtt ggcctgaata aatgtaattt attcgctatt
     2221 gtataatcgt ggccttgaaa ctgttgcgcc cttgtcactg tgcagcctgc aggcgcaata
     2281 atattatttc ggggcgttgt tggcgcccgg atcgaactgc gatcatcgag gtcttcatga
     2341 aacgtgtgtt ttccagagcg ttgcttgccg gcggtttatt gctggcgtct tgcgtggcat
     2401 cggtcggcta tgcggccgaa accgctgtac ccaaacccga tgcggcaaag ggcgaacaac
     2461 tctacgtcaa gggcgacgcg tcgcgcggca ttcttgcctg cgtgacctgt catggcgctg
     2521 cgggcaatag cacgattgcc gcgaatccga atttgtcggc gcaggcgcat gagtatctgg
     2581 tcaaacaact gacggatttc aagtccaaag acggcaaggc ggcgccattg cggcgtggcg
     2641 agggcggcgc caacacggtt atgacggcgt ttgcgaccgc ccttacgccg gccgatatgc
     2701 aaaatattgc gtattacctt gcgcaacaac ctctcgatta caaaacggcc ggcacggcca
     2761 ccaacaaggc cagcatggaa ctcggccagc gtatttggcg tggcggg
//
LOCUS       DATCXU010000284         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_552140,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000284 DATCXU010000000
VERSION     DATCXU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552140"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1927)
                     /gene="htpG"
                     /locus_tag="VIR04_06355"
     CDS             complement(11..1927)
                     /gene="htpG"
                     /locus_tag="VIR04_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY9460403.1"
                     /translation="MTQTDTDTSVSSPETLGFQAEVKQLLHLMIHSLYSNKEIFLREL
                     ISNASDACDKLRFQAIDNPDLMQGDSELRIRVEYDKAQRTVTISDNGIGLTRDEAVSH
                     LGTIARSGTKEFFSQLTGDKQKDAQLIGQFGVGFYSSFIVADKVSVLSRAAGVAETDA
                     VLWESDGQGEFSVMQVEKADRGTSVTLYLREGEDDFLNGWRLREILRRYSDHISLPIQ
                     MLKEEWDSEKSEQVKKNEWETINQASALWARAKSDVTDEQYKEFYKHVAHDFEDPLAW
                     THNRVEGRNEYTQLLYIPKRAPMDLWDRDARHGVKLYVKRIFIMDDAEQLLPSYLRFV
                     RGVIDSADLPLNVSREILQESRDVRAIRDGSAKRILSLLEDLSQNSPEKYTEFWEQFG
                     QVLKEGTGEDMANKDRIAKLLRFASTQADSPAQTVTLADYLGRMKEGQDKIYYVSADS
                     YAAASNSPHLEIFRKKGLEVLLLSDRVDEWMLSHLNEFEGKSLVSIAKGGLDLDKLTD
                     EDEKKHQTEVAEAFKPLVERLKTALGDKVKEVRVTLRLVDSPACVVVDQNELSPHLLR
                     MLKAAGQTAPDVKPILEINPEHALISRMKEASDADFNEWALLLLDQAMLAEGAQLGDP
                     ASFVKRMNRLLLNV"
     gene            2093..2177
                     /locus_tag="VIR04_06360"
     tRNA            2093..2177
                     /locus_tag="VIR04_06360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2127..2129,aa:Leu,seq:caa)
     gene            complement(2248..>2471)
                     /locus_tag="VIR04_06365"
     CDS             complement(2248..>2471)
                     /locus_tag="VIR04_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY9460404.1"
                     /translation="GAFLPLMMVLLLPAFALMWGLAGSSLFFLAFLGLIAARAGGAPL
                     LVSIMRVTFWGALAMGITAGVGSLFGVAA"
BASE COUNT          554 a          723 c          632 g          562 t
ORIGIN      
        1 gccgtttcgg ctaaacgtta agcagcaggc ggttcatgcg cttgacgaaa ctggccggat
       61 cacccaactg ggcgccttca gccagcatgg cctgatccag caatagcaac gcccattcgt
      121 tgaaatcggc gtctgaagcc tctttcatgc gactgatcag cgcgtgctcc ggattgatct
      181 ccaggatcgg cttgacatcg ggcgccgtct gccccgccgc tttcagcatg cgcaataaat
      241 gcgggctgag ttcgttttga tcaaccacca cgcaagccgg cgaatccacc aggcgcaacg
      301 tgacgcgaac ttctttcacc ttgtcgccca aagctgtctt caaacgctcg accaggggct
      361 taaatgcttc ggccacctcg gtctgatgct tcttctcgtc ctcgtcggtc aatttgtcaa
      421 ggtcaaggcc acccttggcg atcgacacca gggacttgcc ctcgaattca ttcaaatgcg
      481 acagcatcca ctcgtcgacc cgatccgaca gcagcagcac ttcaagccct tttttacgga
      541 aaatttccaa atgagggctg ttgctggccg ccgcgtatga atcagcgctg acgtaataga
      601 ttttgtcctg gccctctttc atgcgcccta ggtagtcggc cagcgttacg gtttgcgccg
      661 ggctatccgc ctgggtcgac gcgaagcgca ataatttcgc aatccggtcc ttgttggcca
      721 tgtcctcgcc ggtgccctct ttcagcacct gaccgaattg ctcccagaac tctgtgtatt
      781 tttccgggct attctggctc aggtcttcca gcaacgacaa aatccgcttg gccgagccat
      841 cgcgaatcgc gcgcacatca cggctttcct gcaaaatttc gcgcgacaca ttcaggggaa
      901 ggtcggcaga atcaataaca ccgcgaacga agcgcaagta cgacggcaac aattgctcgg
      961 cgtcgtccat gatgaagatg cgtttgacgt acaactttac gccgtggcgc gcgtcgcgat
     1021 cccacaaatc cattggtgcg cgcttgggga tatacaacaa ttgcgtgtat tcgttgcggc
     1081 cctcgacacg gttatgcgtc caggccagcg ggtcttcgaa atcgtgcgcg acatgcttgt
     1141 agaattcttt atattgctca tcggtgacgt cggattttgc gcgagcccat agcgcactgg
     1201 cctgattgat ggtttcccac tcgttcttct tgacctgctc ggacttttca ctgtcccatt
     1261 cttctttcag catttgaatc gggagcgaaa tatggtcgga ataacggcgc agaatctcgc
     1321 gcagtctcca gccgttcagg aaatcgtctt cgccttcacg caaataaagc gtaaccgacg
     1381 tgccgcggtc cgctttttcg acctgcatga ccgaaaattc gccttggccg tcggattccc
     1441 acaaaacagc gtccgtttcc gcaaccccgg ccgcccggct tagtacagat accttgtcgg
     1501 ccacgataaa cgaagaataa aaccccaccc cgaactgacc gataagctgg gcgtcttttt
     1561 gcttgtcacc cgttaattga gaaaagaatt ctttggtgcc cgagcgcgcg atcgtaccca
     1621 ggtgcgatac cgcttcgtcg cgcgtcaggc cgatcccatt atctgaaatc gttaccgttc
     1681 gttgagcctt gtcatattcg acccgaatac gcaattcgga atcgccttgc atgagatcag
     1741 ggttatcgat agcctgaaat cgcagcttgt cgcatgcatc ggaggcattg gagatcagct
     1801 cacgcaaaaa tatttctttg ttgctgtata gggaatgaat catcaaatgc agcaactgct
     1861 tgacttcggc ctggaaacct aatgtttccg gcgaagaaac ggacgtatcg gtatcggttt
     1921 gagtcattat tgaaccacgc aaaaaataag ttttaaataa aaccagcaca tcccggccga
     1981 tggctggcga ctgatcgttg cctgcataat gggggcaatg cgcaaatttt caagtatgcg
     2041 tataattgcg tcgttctgcc gcgccccggt gacaggggcg atctgcttgc tagcccgggt
     2101 ggtgaaattg gtagacgcag gggactcaaa atcccccgcc gcaaggcgtg tcggttcgat
     2161 tccgaccccg ggcaccactc taaaaacgtg agttacgact ctcagacgct gtatttcgct
     2221 cgccctgcgg cgccgaagcc gtgtatctta agctgccacc ccaaacaggg atcccacccc
     2281 agcggtaatc cccattgcca atgctcccca gaaggtcacg cgcatgatgg acaccagcaa
     2341 gggggcgcct cctgcgcgtg cggcaatcaa tcccaggaac gccagaaaga agagtgaact
     2401 gcccgcgagg ccccacatga gggcaaaggc cgggagcagg agaaccatca tcaaaggaag
     2461 aaaggcgccg g
//
LOCUS       DATCXU010000285         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_562775,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000285 DATCXU010000000
VERSION     DATCXU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562775"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VIR04_06370"
     CDS             <1..907
                     /locus_tag="VIR04_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460405.1"
                     /translation="MRRVGILLPATADDSDYQAFVAAFLQALGQLGWIVGRNMRIDTR
                     WASAEAAAIRRHAAELVALAPDVILAQGSVTVGPLLQTTRTVPIVFPIVADPVGAGFV
                     ESLARPGGNATGFMDFEYGMGGKWLELLKEIAPSVTRTAIVRDTAIPSGIAVFGIIQA
                     MAPSLRMEVIPINIGDAGEFERSVAAFARVPNGGLVVTPSAQTLLHRNLIVTLAARYK
                     LPAIYPGRTFAAGEGLVSYGADFLNQYRQAAGYVDRILKGEKPGEMPVQAPTKYELVV
                     NLKAAKAIGLTVPPTLLARADEVIE"
     gene            1373..1567
                     /locus_tag="VIR04_06375"
     CDS             1373..1567
                     /locus_tag="VIR04_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460406.1"
                     /translation="MSATVKHVLNAENIKKLLDYDPLTGLFTWRWRDKKYFPADRYWR
                     IWNKRRAVTYSAGDGSQTGH"
     gene            complement(2706..3296)
                     /locus_tag="VIR04_06380"
     CDS             complement(2706..3296)
                     /locus_tag="VIR04_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460407.1"
                     /translation="MNLNWGVLRLWLAISGIWMILVMGITSYGSAAPISYLKFYRPLS
                     VTDSGNVLYLDKPEWKPALTMVYPPRRELYVLDGVGWRKLSSPQYTEIARAIDSALRG
                     ITDTGEKDETWRTVRITDMNGSKTDVRVSDDFEQLSPREQAQRVDEFIELVRSPTDKF
                     FTYTFWAMLIAPPVAAGILLFLIAWGLSGFRARAST"
     gene            complement(3388..>3569)
                     /locus_tag="VIR04_06385"
     CDS             complement(3388..>3569)
                     /locus_tag="VIR04_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460408.1"
                     /translation="ATLREAKAVVTQYHQYLKENSLIKSMFEPDILPPAELKRQNKDE
                     RGGRGVTESDIAPTE"
BASE COUNT          796 a         1006 c          991 g          776 t
ORIGIN      
        1 gatgcggcgt gtcgggatac tccttcccgc aaccgcggat gattcggatt atcaagcctt
       61 tgtcgcggca ttcctacaag cgctagggca attgggttgg atcgtcggcc gcaacatgcg
      121 gatcgacacc cgctgggcca gcgccgaggc agccgccatt cgcagacacg ccgcagaatt
      181 ggttgcgctt gcgcccgacg ttatcctggc tcagggcagc gtgaccgtgg ggccgttgtt
      241 gcagacgaca cgcacagtgc caattgtgtt cccgatcgtg gccgatccgg tcggcgccgg
      301 cttcgtcgag agcttggcgc ggccgggggg caacgccacc ggttttatgg atttcgagta
      361 tggcatgggc gggaaatggc tggagttgct caaggagatc gcgcccagcg tgacacggac
      421 agcgatagtt cgggatacgg ccatcccctc cgggatcgcc gtgttcggta tcatccaggc
      481 tatggcgccg tcgctccgga tggaggtgat cccgatcaat atcggcgacg ccggcgagtt
      541 cgagcgctct gtagcggcct tcgcgcgcgt tccaaatggc ggtctggtcg tgacgccgag
      601 tgcgcagacg ttgcttcatc gtaatctgat cgtcacgctt gcggcacggt acaagctgcc
      661 cgcaatctac cccgggcgca cattcgccgc cggcgaaggt ttggtttcct atggagccga
      721 cttcctcaat caataccggc aagcggccgg ctacgtcgat cgcatcctca agggtgagaa
      781 gcccggcgag atgccagtgc aggcgccaac taagtacgag ctggtagtca atctgaaggc
      841 agcgaaggct atcggcctga ctgtcccgcc cactttgctc gcccgcgccg acgaggtgat
      901 cgaataacga ggcgttgtcc gcttttggcc cgaagcagac atccgcagtt gcgccgcaca
      961 tgtccgcttt cgggggtaaa tgcggaattt gaatcatatc aacgcgttgc agtggcaaac
     1021 caaaaactaa tgcgtcgctg atgctcatag cttttcgcgg aaagtggcaa acttttttgc
     1081 gggcatagcc ggtcaccgac tcttgccggg gagtcgaatc ccggccatat aaaacgcgct
     1141 gtgaatccat tttttcaact atctgcatga aataattgaa taaacggcaa atcatgccca
     1201 cgcttaggga aaacaactga caaacacagc cgaatgaaca cagagtttaa tcgaagcatc
     1261 aagcgcctca ccgattccga agatcatcgc gcccgcacga ccggattcgg gacaacggac
     1321 gtactgcgac ggcgcggggg caatcttttg tcaaaaacaa aaaggaatgc gcatgagcgc
     1381 cacggtaaaa cacgttctga atgccgagaa tattaaaaag ctgctcgatt acgatcccct
     1441 aacgggactg ttcacttggc gatggcgcga taaaaaatac ttccccgccg accgctattg
     1501 gcggatttgg aataaacgtc gggccgtaac gtattcggcg ggcgatggct cacaaaccgg
     1561 acactgaaac gtgcgcttga tcttatccgg tgcaatgggc aagaccctga caagccacat
     1621 ctcctcaccg cacacgggac agcggggccg gctgtcaagc aacgatactg aggttaggca
     1681 gcggtcggta tggggcatag cgctcctcct gttttgcagg cgggagcgcg attggtctct
     1741 cagtcaccgg tgcccaaagg tttcgttatg atttttctct gccagtgatg cgatcagcgt
     1801 gcgcttatat ttggcgaata gctacaacaa aaagaacatt cctcgctgtg tttggcgtga
     1861 taccaaatga aatgggccgc acctttcagc gcggccccta ataaatgcga tcaacgtatt
     1921 gttgggcgca cgaaattaac cgcagaccca aacgaagcca atcgggctcg gccgccaaca
     1981 ttcgcctacg ccatatggcc accaccggcc gccccagtag cgccgtccgg tgccgtacca
     2041 aacgcctccg taatagcgtt ggccgacgcg atatccgcca tgagggccgc gcaccgccac
     2101 attgccatgt gggccacgat aagcctctcc accacggggc ccacgataag cctctccgcc
     2161 acggggccca cgatagactt cctgggccaa ctggatatct gacgcgggag caacagccct
     2221 gagcggttgc ggcttgattg ggacagcatg ggccgtattg tagtctgtga tcgcgagcat
     2281 ggctgcgacg gcgaaaggta acgtccaaac actacgcatc tctaaccttc cttttcgctg
     2341 ttgttaaaaa tccggtattg gcgcgccctt gccccggatt tcggcccctc agattaagat
     2401 gggcacatga acgcgaactg aacagcatgg tgcaacagcg ccagtgcagg gaacgtaacg
     2461 ccacatggaa tggttgagcc agaaaacatc gatagcgggc attgaaattt caaattgggc
     2521 actagttctg gccgccctca tcatactctt ggtcgtcttc gcacaattga aataagttca
     2581 gccccgcggc agctcttctt cttttggggc tgaggctgta tatcgacgcc atgggcgtgt
     2641 gcgtaattca cggtgtttat ccgttcacac cgatgcgcaa aacccgtcgc cgggcgcgaa
     2701 acgcactaag ttgatgcccg cgcccgaaag ccggaaagcc cccatgcaat caaaaacagc
     2761 aaaattccag cggcgaccgg cggtgctata agcatcgccc agaaggtgta tgtaaaaaac
     2821 ttgtctgtgg gagatcgtac taactcaata aattcatcga ctctttgcgc ctgctcgcgg
     2881 ggcgaaagtt gctcgaaatc gtcgctcacc cgtacgtcgg ttttagagcc gttcatgtcc
     2941 gttattcgca ccgtacgcca agtctcatcc ttttcgcctg tatcagtgat gccccttaat
     3001 gcactgtcga tggcgcgggc gatttccgta tattgtggtg agcttagttt tcgccatccc
     3061 acgccgtcga gtacataaag ttctcgccgc ggcggataca ccatcgtcag tgcgggtttc
     3121 cactcgggct tgtcgagata aagtacattg ccgctatcgg tgacggataa cggtctatag
     3181 aactttagat atgagatggg tgccgcgctt ccgtaagaag tgatccccat tacgaggatc
     3241 atccaaatcc cagaaatggc aagccacagc cgaagcacgc cccaatttaa attcatttcg
     3301 tgcaaccgcg ccttaaaata tcgcattgcc tgttcgccgc taggcgcgta gccgtgatta
     3361 tagactgatg gcggttgctg tccggggcta ttcggtcggt gcgatatcgg actcagtaac
     3421 gccgcgaccg ccgcgttcat ccttgttctg ccgttttaat tccgcgggag gcaaaatatc
     3481 aggctcgaac atagatttga tgagcgagtt ttcctttagg tattggtggt actgagtcac
     3541 aacggctttt gcttctctca gtgtggcgg
//
LOCUS       DATCXU010000286         6315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_969162,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000286 DATCXU010000000
VERSION     DATCXU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969162"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VIR04_06390"
     CDS             <1..576
                     /locus_tag="VIR04_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460409.1"
                     /translation="NAIAGLIPVAGGTVHFRDEPIAGKPAYAIVRKGLALSPEGWRLF
                     VRQTVENNLLLGATTVSSKSTVTDMISRVYTLFPKLAERRHQRAGTMSGGERQMLAVG
                     RALMSNPKMLLLDEPSLGLAPAIVESMYETLTQLHHTGLTILLAEQSVELALEVSDHA
                     YVLQVGHSILSGPSTTLARDPEVQRAYLGLR"
     gene            880..2220
                     /locus_tag="VIR04_06395"
     CDS             880..2220
                     /locus_tag="VIR04_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460410.1"
                     /translation="MAHNDISRSDSTSTLAAVACCPPLEPCAVCDVLNFTYRLPFRPV
                     IAAGDQRQTVPVEVTLHYRLTRCAGPLSLGDLKYTTTLLPGEEVRLSSSDRHTRFSFD
                     SETNLSYRNESTSEESYYAAGMAHGMSNLNVLDTTNASSSFSSSSVSGGGGAGIDLGF
                     VSIGGSVAANSHDANSTSALAHSLSQHAESLQQHMEVSTRAAASTSIGEVATRNHQQS
                     ESDDQYESASRMFKNPNHCHAITFFFYGINKCQTVKFELVAIDRRVNDPLAPTGAVLN
                     QPKPATGVAIHSSAVLATGANRLDVARRALDSVAVEQADTAAAVTLLGRQFAALAQQT
                     PIPVSIRQAALQQVDQDLVKEGLIDKVGGTVSPTMQKKLGWEKTMAIPTPGILVKGSL
                     DNCDICEPELQQQIALDLVRQDLENQLLKKQIDLLEKSQEYRCCPADEIESSKP"
     gene            2281..3048
                     /locus_tag="VIR04_06400"
     CDS             2281..3048
                     /locus_tag="VIR04_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY9460411.1"
                     /translation="MAYTVKHYGWLRDIPDHRDYLYAAPVAALPVLSSSVDLRNQCPP
                     IYDQGALGSCTANGIGAAMQFERAKQKLEPDFIPSRLFIYYNERVMEHTVKSDSGAMI
                     RDGIKSVAKQGDCLEDEWPYIIEKFTTKPPQKCYKNALHYTVLQYQRIIQNLGQMKGC
                     LASGYPFVFGFTVYESFESQQVAKDGVVPMPGPSEQLMGGHCVVAVGYDDSQQRFIVR
                     NSWGESWGLSGYCTMPYAYLIDPNLASDFWTIRLISA"
     gene            3109..3438
                     /locus_tag="VIR04_06405"
     CDS             3109..3438
                     /locus_tag="VIR04_06405"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEY9460412.1"
                     /translation="MAELTLTQLDKGKSVTICPGEIVRISLDENPGTGFRWELEQEDD
                     QILHLLASHFVGSASAGVGGGGHHIWEFMAKKNGNVRLTMKHWRAWEGCKSTIERLDF
                     AIQIKAN"
     gene            3539..3973
                     /locus_tag="VIR04_06410"
     CDS             3539..3973
                     /locus_tag="VIR04_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9460413.1"
                     /translation="MNVRTLILGAMVAAFATIVNPDAASAAEPATPDQGVTQAFRQAI
                     ANVPGKTLTGVIVNYAPGVKSPPHRHGHAFVVAYVLSGAIRSKVNNGEERIFHAGESW
                     TEMPGDHHVVSANASDTEPAKLLAIFVADTADNDLVIWDKQK"
     gene            4054..5139
                     /gene="ada"
                     /locus_tag="VIR04_06415"
     CDS             4054..5139
                     /gene="ada"
                     /locus_tag="VIR04_06415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY9460414.1"
                     /translation="MKPDIHPVQDRRNAIEKDPRWSAILNRDPTADNKFVYAVKTTGV
                     YCRPDSPTRRPKPENVEFFDSPEAARRAGYRPSRRAPEDKAMVAAQHAALVSNACRYI
                     ETADNIPTLNELAQRANMSRYHFHRVFKSTTGLTPKSYAGAHRARKMRNELGGKESIT
                     DAIYDAGFNSNSRFYEASNHRLGMRPKDYRAGGANTEILFAAGECSLGSIVVAQSERG
                     VCAILMGDDPDQLVRDLQDQFPKASLVGAVAGFERVVAKVVGLIDAPALGLDLPLDIQ
                     GTAFQERVWRALREIPAGATATYSDIAHRIGMPEAARAVARACSANPLAVAIPCHRVV
                     RKDGQLSGYRWGVERKRTLLDREAPQS"
     gene            5195..>6315
                     /locus_tag="VIR04_06420"
     CDS             5195..>6315
                     /locus_tag="VIR04_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY9460415.1"
                     /translation="MLYNLHIYPKGAAHQISRDARLDTFHEVRPLMRVNYVEPLVMLA
                     TVVRWSFLSIIMGTIAGTGTSLFLHALFYSSAQTAHIPLWFQMVLLPIAGILNGLILY
                     YGYRKSGDEMKDSVIAAVHTRAGIMPVKTFLIKPIAAVITLALGGSAGKEGPCSHIGG
                     TLASWFGRLIRLDPEMQKRIVACGVSAGFASVFGTPIAGAIYGVEMLAIGRIRHDFIF
                     PAIIAAIVSFETSKFWGITYDYFPLNITVDFSELLFTKIILIGIVCGLSAWLFIELLE
                     KSRSLFTFIQHRFNIWRPLVPFLGGLVLAVLILIIPTDYLGLSLPVMNDALHGKDIAL
                     LGFLWKSLLVAITLGSGFYGGIVTPQFVIGALCGNAFAGL"
BASE COUNT         1462 a         1824 c         1702 g         1327 t
ORIGIN      
        1 aacgccattg caggtctcat ccccgtcgct ggcggcacag tgcactttcg tgatgagccg
       61 attgccggca agccggccta cgccattgtg cgcaagggtc ttgcgctgtc gcccgaaggc
      121 tggcgcctgt ttgtgcgcca aacagtcgaa aacaaccttc tgctcggagc cacgaccgta
      181 tcaagcaaaa gcaccgtgac cgacatgatc agtcgcgtct acacactttt tccaaagctc
      241 gcggaacgcc gtcaccagcg cgcgggcaca atgtccggag gcgaacggca aatgctggcc
      301 gtcggccggg cactgatgag caatccaaaa atgctgttgc tcgacgaacc cagccttggg
      361 ctggctccgg ctatcgtgga aagcatgtac gaaacgctta ctcaactgca ccataccggc
      421 ctgaccatcc tgctggcgga acagtcagtc gaattggcgc ttgaagtgtc ggaccatgca
      481 tatgttttgc aggtcggtca cagcatcttg tcgggtcctt caaccacgct cgcgcgcgac
      541 cccgaggttc agcgcgccta tctcggattg cgctgaccca atctcttgcg cggcaagcgt
      601 cgcgcattcg aacggccgta tcaaccggta agaaccgctt tattttgcga cagtcgtcga
      661 ctttctcttt cggaggaaga taatacgcgg gaaacagctt catctgtcga aataccaccg
      721 tagttcgctg ccgcgcgtct ttgtgttggc ggctaaaacg tagctcgaag tcgggaacaa
      781 aaaattaaca tcccacaaca cggcactggg gacaggtggg aatgagtgcc atccgcggat
      841 tcatctgccg aacacccaga tagtcacaca ggagcaatca tggcccataa cgacatatcc
      901 cggagcgatt ccacgtccac cctggcagcg gttgcctgtt gcccaccact tgagccctgc
      961 gcggtttgcg acgttctcaa tttcacctat cgccttcctt ttcgtcccgt catcgccgca
     1021 ggagaccaaa gacaaacggt acctgtcgaa gtcacgctcc attacaggct gacgcgttgt
     1081 gcaggcccac tttcgctcgg cgatctgaag tacaccacga cgctattgcc cggcgaagaa
     1141 gtgcgactta gctcaagcga ccgccacacc cgattcagtt tcgacagcga gacaaaccta
     1201 agctatcgta atgaatcgac atcagaagaa tcctactacg cagcaggcat ggcccacggc
     1261 atgagcaatc ttaatgtgct ggatacaacg aatgccagtt catccttcag ctcatcgtca
     1321 gtcagcggag gcggcggcgc gggaattgat cttggtttcg tcagcatcgg cgggtcggtc
     1381 gccgccaatt cccacgacgc aaattccacc agcgcgctag cgcattcgct cagccagcac
     1441 gcggagtcgc tgcagcaaca catggaagtc agtacccgtg ccgctgcgtc gacatccatt
     1501 ggcgaagtgg ccacccgcaa tcaccaacag agcgagtcag acgaccagta cgaatcggcc
     1561 tcgcgcatgt ttaaaaatcc caaccactgc cacgcgatta cttttttctt ctatggcatc
     1621 aacaagtgcc aaacggtcaa attcgagttg gtcgccatcg atcgccgcgt caatgacccc
     1681 cttgccccca ctggcgccgt gctaaaccag ccaaagccgg cgaccggagt cgccatacac
     1741 tccagcgcgg tgctcgcaac aggcgcaaat cgtctcgacg tggcacgccg cgctctggac
     1801 agcgttgcgg ttgaacaagc ggacacggcg gctgcggtga cgctgctcgg gaggcagttc
     1861 gcggcactgg cgcagcagac gccaattccg gtaagcattc gtcaggccgc tttgcagcaa
     1921 gtcgatcagg acctggtgaa ggaggggctg attgacaaag tgggaggcac ggtttctcca
     1981 accatgcaga aaaagctggg atgggagaaa acaatggcca tccccacgcc tggaatcctt
     2041 gtaaaaggct ctctggataa ttgcgacatc tgcgagcctg aacttcagca gcagattgca
     2101 ctcgacctgg tgcgccagga ccttgagaac cagcttctga agaagcaaat tgacctgctg
     2161 gaaaaatcgc aagaataccg atgctgcccg gcagacgaga ttgaaagctc taaaccctag
     2221 ctgcaaaacg caaggactgg aacagaaaat ttcgactgac cgcaatccaa ggatatcgcc
     2281 atggcttata ccgtcaaaca ttatggatgg cttcgcgaca tacccgatca tcgcgattac
     2341 ctttacgccg cgcctgtcgc cgctttgcct gttctttcct caagcgtgga tctgcgcaat
     2401 cagtgtccgc ccatatacga tcagggagca ttgggcagtt gcacagccaa tgggatcggg
     2461 gcggcaatgc aattcgaacg agcgaaacag aaactcgaac ccgatttcat tccgtcccgg
     2521 ctcttcattt actataacga aagggtaatg gagcacaccg tcaagtcaga cagcggcgcg
     2581 atgatccgcg atggaatcaa aagcgttgcg aagcaaggag actgtctcga agacgagtgg
     2641 ccctacatta ttgagaaatt caccaccaag ccgccgcaaa agtgctacaa aaacgcgctt
     2701 cattacacgg tgcttcaata tcaacggatt attcaaaatc ttggtcagat gaaaggctgt
     2761 ctcgcctcgg gctatccgtt cgtattcgga ttcaccgttt atgaaagctt tgaaagccag
     2821 caagtggcga aagacggggt ggtgcccatg ccggggccat cggaacaact gatgggcgga
     2881 cactgtgtcg tggcggtcgg ctatgacgat tcgcaacaac gcttcatcgt acgcaattca
     2941 tggggagaaa gctggggttt gtcgggctat tgcacgatgc cctatgccta cctgattgac
     3001 ccgaaccttg cttctgattt ctggacgatc cggctcatct cagcctgaca aaagtggcac
     3061 aatagattca acattgaccg ggacgccttg ccgcgcaagg aaaagacctt ggctgaatta
     3121 acgctgacac aattggataa ggggaaatcg gtcacgatct gccccggcga aatcgtacgc
     3181 ataagccttg atgaaaatcc tggcaccggt ttccgctggg aactggaaca ggaagacgac
     3241 cagattctcc atttgctggc ttcgcacttt gttggcagcg ccagcgcagg tgtcggcggg
     3301 ggcggtcatc acatttggga attcatggct aaaaaaaatg gcaatgtcag gctgacgatg
     3361 aaacactggc gtgcctggga gggatgcaag tccacgatcg aacggcttga cttcgccatt
     3421 caaataaaag caaactaatc taacgcaatg ccggcacttg ccccggcacc aagcgcgatc
     3481 catggcgccg caccgtccgc cgaccaggcg gcaatcaggc aaattacggg aaatcaacat
     3541 gaatgttcga acattaatac taggcgcgat ggtcgccgct tttgccacta tcgtaaatcc
     3601 tgacgcggca tccgcggcgg agcccgcgac accagaccaa ggtgtcacgc aggcgtttcg
     3661 acaggccatt gcaaacgtcc ctgggaaaac gctgactggt gtcatcgtga actacgcacc
     3721 gggcgtcaaa tcaccgccac atcgtcacgg ccacgctttc gtcgttgcgt atgtcctgtc
     3781 gggcgcaatt cgcagcaagg tcaataatgg cgaggagcgt atttttcatg cgggcgaaag
     3841 ctggactgaa atgcccggag atcaccacgt ggtaagcgcc aacgcaagcg ataccgagcc
     3901 cgcaaaattg cttgccatct tcgttgcaga caccgctgat aacgacctgg tgatatggga
     3961 caagcagaaa taatttgacc gcaaggaccg gagtgttctc gcggcggaac cgccctacag
     4021 tgcacctgtc agacaagcaa tgcggaacac gccatgaaac ccgacatcca tcccgtacaa
     4081 gaccgacgaa acgcgattga aaaggatccc cgttggtctg ccattctaaa ccgtgacccg
     4141 acggctgata ataaattcgt atatgcggtc aagacgaccg gcgtttattg ccggcctgac
     4201 agccccacac gccggcccaa gccagaaaat gtggaatttt ttgacagccc cgaagcggct
     4261 cgacgcgcgg gttaccggcc cagtcgccgg gcgccggaag acaaggcgat ggtcgccgcc
     4321 cagcatgcgg cccttgtctc gaacgcatgc cgctacattg aaacggccga caacataccc
     4381 acgctgaacg agctggcgca gcgcgcgaac atgagccgct accactttca tcgggtattc
     4441 aagtccacga ccggccttac tccaaagtcc tacgcgggcg cgcaccgggc gcgaaagatg
     4501 cgtaatgaac ttggcggtaa agaatccatc acagacgcaa tctatgatgc cggtttcaat
     4561 tcaaacagcc gcttttacga ggcatcgaat catcggcttg gcatgcgccc gaaagactat
     4621 cgcgccggtg gcgccaacac ggaaattctg ttcgcagccg gtgagtgttc attaggttcg
     4681 atcgtcgtgg cgcaaagtga acgtggcgta tgtgcgatcc tgatgggcga cgaccccgac
     4741 caactggtgc gcgaccttca ggatcagttt cccaaagcca gcctggtggg cgccgttgcc
     4801 ggctttgaac gggtggttgc caaagttgtt ggattgatcg acgcgccggc cttgggtctg
     4861 gacctgccgc tagacataca aggcaccgca ttccaggaac gggtttggcg ggcattgcgc
     4921 gaaattccag cgggcgcgac cgctacgtac tccgacatcg cccatcgaat tggcatgccc
     4981 gaagcggccc gggccgtcgc gcgggcatgc agcgccaacc ctctggcggt cgccatccca
     5041 tgccaccgcg ttgtgcgcaa agatggccag ctttcgggtt atcgctgggg agtggaacgc
     5101 aagcgcacac tgctggatcg cgaggcgccg cagtcctgac tccgtcccgc aaaagggttg
     5161 cgcatgctca tcagcgcatt gagggtgtag ctgcgtgctc tacaatttgc atatttaccc
     5221 aaaaggagct gcgcatcaaa tatcccgcga cgcccgcctc gacacattcc acgaggtgcg
     5281 ccctttgatg cgcgtcaatt acgtcgagcc gcttgtcatg ctcgcgacgg tcgtgcgatg
     5341 gtcttttctg tcgatcatta tgggaaccat tgcaggaact ggaaccagcc tttttctgca
     5401 cgctctcttt tattcgtcgg cgcaaactgc ccacatcccg ttatggtttc agatggttct
     5461 attgcccatt gccggcatac tgaatggact gattctttat tacggttatc gaaaatcggg
     5521 cgatgaaatg aaggattccg tgatcgccgc cgttcacaca cgcgccggca tcatgcccgt
     5581 taaaacattt ctgattaagc cgattgcggc cgtcattacg cttgcactgg gtggatcggc
     5641 tggcaaagaa ggcccctgct ctcacatcgg aggcacgctt gcgtcatggt tcggacggct
     5701 gatccggctg gatcccgaga tgcaaaagcg gatcgtcgct tgcggagtaa gcgcgggctt
     5761 tgccagcgtt ttcggcaccc ccatcgccgg cgcaatttat ggcgtcgaaa tgttggctat
     5821 agggcgcatt cgccatgatt tcatttttcc cgccatcatc gccgcaattg tctcgtttga
     5881 aaccagcaag ttttggggca ttacatacga ttactttccg ttgaacatca ccgtcgattt
     5941 ttcggaactt ctcttcacaa aaatcatcct tattggcatc gtatgcgggc tttccgcctg
     6001 gctgttcatc gaacttcttg aaaaaagccg atcgttattc acattcatac agcatcgctt
     6061 caacatctgg aggccgctcg tgccattcct gggcggtctg gtgctggcgg tccttatcct
     6121 gatcatcccg acggattatt taggtcttag cctgcccgtg atgaacgacg cattgcatgg
     6181 caaggatatt gctctgctgg gattcctgtg gaaatcgttg ctggtggcca ttacattggg
     6241 gtcgggtttt tacggcggca tcgtcacgcc ccaattcgta attggagcgt tgtgcggaaa
     6301 tgcctttgcc ggcct
//
LOCUS       DATCXU010000287         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_789502,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000287 DATCXU010000000
VERSION     DATCXU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789502"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VIR04_06425"
     CDS             <1..523
                     /locus_tag="VIR04_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9460416.1"
                     /translation="RRQGVRRVVFASSNHAVGYHRRERFLDNTVQPRPDSRYGVSKVF
                     GEAVGRMYADKFGLSVACLRIGTFRVPDAPVEPRQLLTWISHRDMAQLVQRCIESPSY
                     HFVVVYGVSNNLRNRWDNSSVKFLGYQPEDNSEVFAARILSESKPENAISAQFHGGYF
                     CPMEFVGDTDRIE"
     gene            596..1897
                     /locus_tag="VIR04_06430"
     CDS             596..1897
                     /locus_tag="VIR04_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460417.1"
                     /translation="MKKTKPWLMAGAAGLVLCAGSIGGAMAAQQELRIAYQPNPLQEA
                     SIDMMVKWGAKNNVKIVKVPNSYGVYVEKMTASLTSGSQQYDVIWHNDDWGQLWKHLL
                     EPLDDVAGLKYADKWSTDPIIFNNKDGKTTVVPMGETFGAFFYRTDLVKESELPKTWD
                     QLVTLSKKLQAEGKVKFGFVGGMNVTADWNSTLWTLWNNNCDVLKPFYSRDNAELAKA
                     GWESALNEPCTRQVAEFWSDAIKKDKIIPPGMPAYDRNEANAIFTAGNAAFTVADTTY
                     WNTFNDPAKSKVAGKVGAAQFPLGPMGKEVRNWDDAWGWAIPKAVSAEHKALAKKMLS
                     DMMMDEEGQIALWKKTGAPPPNVTFWATIAKTDSFMRFLQKYYLDNPNKVRGSYYFPD
                     WPAGHKAYNNAMTTAILGNRDDIPKALAAGAENIRSAIQQK"
BASE COUNT          485 a          518 c          582 g          425 t
ORIGIN      
        1 ccgccgtcag ggtgtgcgtc gtgtcgtgtt cgccagttcg aatcatgcgg tgggctatca
       61 ccggcgcgaa cgctttctgg acaacacggt gcagccgcgt cccgactcgc gctacggcgt
      121 gtcgaaggtg tttggcgagg cggtaggccg aatgtatgcc gacaaattcg gcttgtcggt
      181 ggcgtgcttg cgtattggca cattccgcgt tcccgatgcg ccggttgagc cgcgtcaatt
      241 gctgacctgg atcagtcatc gcgatatggc ccagcttgtg cagcgctgca ttgaaagtcc
      301 cagctatcat tttgtcgtgg tttacggggt gtcgaataat ttgcgcaatc gctgggataa
      361 ttcctcggta aagttcctgg gttaccagcc tgaagataat tccgaagtat ttgcggcgcg
      421 cattttgtcg gaatcaaagc ccgaaaacgc catatccgcc cagtttcacg gggggtactt
      481 ttgtcccatg gagttcgtgg gcgataccga tcgtattgag tagctctcca gcgaaaagct
      541 ggcgcggtag tcccttataa atagaaagaa ggtcaagcta accaaggagg cagacatgaa
      601 aaagacaaaa ccgtggctta tggcaggcgc cgcagggctc gtgctatgcg ctggcagtat
      661 tggtggtgca atggctgcgc aacaggaatt gcgcattgcc tatcagccca atccgctgca
      721 ggaagcatcg atcgacatga tggtgaaatg gggcgcgaaa aataacgtca aaattgtcaa
      781 agtgccgaat tcctatggcg tgtatgtcga aaaaatgacg gcgtcgctga catcgggctc
      841 gcagcagtac gacgtcatct ggcacaacga tgactggggg cagctctgga agcacctgct
      901 tgaaccgctc gacgatgtcg ccggtctaaa gtacgccgac aaatggagca ccgatcccat
      961 cattttcaac aacaaggacg ggaagaccac cgtggtgccc atgggcgaga ccttcggcgc
     1021 atttttctat cgcacggatc tggtcaagga aagcgagctg cccaaaacgt gggaccaatt
     1081 ggttaccctg agcaagaagc tgcaggctga gggcaaggtc aagttcggtt ttgtcggagg
     1141 catgaatgtg accgccgact ggaactcaac gctttggacc ttgtggaaca ataactgcga
     1201 cgtcctgaag ccgttctaca gccgcgataa cgcggagctg gccaaagcgg gctgggaaag
     1261 cgcgttaaac gaaccctgca cgcgtcaagt cgccgaattc tggtctgatg cgatcaagaa
     1321 ggacaagatc attccgccgg gtatgccggc ttatgatcgc aacgaggcga acgcgatatt
     1381 cacggcgggt aatgcggcct ttacggttgc ggacacgacc tattggaaca ccttcaacga
     1441 cccggccaaa tccaaggtgg ccgggaaagt tggcgcggcc cagttccctc ttgggccgat
     1501 gggcaaagag gtgcgaaatt gggatgatgc atggggttgg gctataccaa aggctgtctc
     1561 agcggaacac aaggcgctgg ccaagaagat gttgtccgac atgatgatgg acgaagaagg
     1621 tcagatcgca ctttggaaaa agacgggtgc gccaccaccc aatgtgacgt tttgggccac
     1681 gatcgccaag accgattcct tcatgcgatt cctgcaaaaa tactatcttg ataaccccaa
     1741 caaagtccgc ggttcttatt attttccgga ctggccggca ggtcacaagg cttataacaa
     1801 cgccatgacg acagcgattc tcggcaatcg agacgatatc cccaaggcgc tggctgcagg
     1861 cgccgaaaac attcgcagcg ccatacaaca aaagtaaaag gatagacata tgtatgctgc
     1921 gccccccgtt attgccacta ctgtctttgc gcgtatccct gataagtttc gcattcatgg
     1981 ccgcagcaac cgctggatcg atgtgcagcg
//
LOCUS       DATCXU010000288         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_912907,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000288 DATCXU010000000
VERSION     DATCXU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912907"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VIR04_06435"
     CDS             complement(<1..684)
                     /locus_tag="VIR04_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9460418.1"
                     /translation="MTDPTSISSDVLLVEMQGAMAIITVNRPEKLNALNDAVLAALQG
                     ALDALEKDPSVKVLIFTGTGEKAFVAGADIEQLAQMTTSQAFERMTAIHSLFLRISDF
                     SKPTIAMVNGYALGGGFELALSCDMVVASENAVFGFPEINLDTLPGWGGTQFAPKKMG
                     LNRAKEMVLTGNYYPAAVCVGFGFINHLVPAAQLRSATFELAGPIASKNAFALKMAKS
                     ALQQAVGSDL"
     gene            981..1883
                     /locus_tag="VIR04_06440"
     CDS             981..1883
                     /locus_tag="VIR04_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY9460419.1"
                     /translation="MKNTGSNCIPIYCIGDYGETVKSTEFDFARLERRLEVLPYPHRH
                     DFYHIVWVTHGDGNHIIDSVSYPVKSNVIFFLAPRQIHDLDLPEDTTGFTINFSPEFF
                     FLNPTNRNISSHSVFYGANERVQALYLTDEQAESLKNIIKEIESEYYSEFANRYEIIR
                     AYLYIFLTHAERYACQAKHAAITPHSFALTQKFKALLEAEHLSIGSVQRYAERLHITP
                     QHLSEATKIATGLSATQLMHQHLVLEAKRMLGHSNSNVTWIATQLGFDDPGYFSRFFK
                     KHVGQTPREFQKNVRPTVDRRRNM"
     gene            2056..3270
                     /locus_tag="VIR04_06445"
     CDS             2056..3270
                     /locus_tag="VIR04_06445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9460420.1"
                     /translation="MYGLEGIRVISFNHFLIGPVGVQYLADLGADVISIEPIDGAFQR
                     KWGGTGKKVDGQSMLHLVGNRNKRSLSLNLKDPRGIEIVKRLIATADVITENYRPGVM
                     NKLGLGYDDAKAIKPNIIYAVASGFGADGPYVNRPGQDLIIQAMSGLAAVTGNHANGP
                     RAVGVSAADHHGAALYAMGILAALLRRERGGEGGRVDVNLLSSAIDLQQESFTCYLNG
                     ERPQDINQPGHVAGWCFPAPYGIYASRDGYLAISLGSLSLLYDILGVPDTERIAQEAE
                     FTQQKTISELLARYLIQGSTAHWVQLFQENKIWHSPVNDYEQVIEDPQVKHLHSFNSL
                     TGATGAPITLVAHPVRFDGQTPGVRLAPQKLGAQTVAILGELGFSGDDISKLHDAGVV
                     NWSDASAETPLH"
     gene            3345..3806
                     /locus_tag="VIR04_06450"
     CDS             3345..3806
                     /locus_tag="VIR04_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY9460421.1"
                     /translation="MSSMSIQIGETVTFEKTVGETDVYMFAGITGDFSGNHVSEAYMR
                     TSKYGHRIAHGALMVGFMSTASSALIDRYLARGMRDTVPVSLGYDRIRFTAPVFFGDT
                     IRVTYRVVEADEERLRTRATIEVAKDDGTVVAVGEHLMKWLPRETTERGDK"
     gene            3821..>4740
                     /locus_tag="VIR04_06455"
     CDS             3821..>4740
                     /locus_tag="VIR04_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY9460422.1"
                     /translation="MQENSANCAPDPGSLRLFEQTLDRAAQKVAEDEFTLGVEFPHVT
                     NPDGSWVRLPASLSAGYTGQTWSHGNWLCGFWVGLLLASYLRTGDKHYLVWARERMRL
                     VAQRADDPNTHDIGFIFNSSAIPGFHITGDTWYADIALQAANRLRARVVTTPNGAYIA
                     SWGPMNDTRGRRSSAIDTMANLPLLYWAANYSGDGSFRLVADAHAAMTARGFIRPNHS
                     TYHAVEYNDTTGERIRGYTFQGAFDESDWSRGQAWAIYGYVQSAHETRKQEYLQLAEQ
                     SANYYLKRLGGRSVPPWDFDATGEDAKVKDT"
BASE COUNT         1200 a         1319 c         1232 g          989 t
ORIGIN      
        1 aagatccgac ccgacggcct gctgcaaggc gctcttggcc attttgagcg caaaggcgtt
       61 cttcgatgca atcggcccgg caagttcgaa cgttgcggaa cgcaattggg ccgccggcac
      121 aaggtggtta ataaaaccaa agccaacaca aacagccgcg ggataataat tgcccgtcaa
      181 aaccatttct ttagcgcgat tcagtcccat cttttttggc gcgaactgtg tcccgcccca
      241 gcccggcaac gtatcaagat tgatttcggg aaaaccaaat acagcatttt cggaagccac
      301 caccatatcg cacgaaagag caagctcgaa tcctccgccc aaggcatagc cattgaccat
      361 tgcaatggtc ggcttcgaaa aatcactaat gcgcaaaaac agactatgga tggccgtcat
      421 tcgctcgaag gcctgcgagg tggtcatttg agcgagctgt tcaatatcgg cgccagcaac
      481 aaatgccttc tcacccgtgc ctgtaaaaat caataccttt accgacggat ctttctcaag
      541 cgcgtcgagc gcgccctgca atgccgcgag caccgcatca tttaatgcat tgagcttttc
      601 aggacgattt accgtaataa tcgccattgc gccctgcatc tcaactaaca atacatctga
      661 cgagatactg gtcggatctg tcatagtggc tggcgttctc gggtgaaatt gaaaattagt
      721 ttatcagcag cacacatctg ccgctaggac aatttttaga atttattgca cttattaacg
      781 gccatcccgc ccgggccccc ttccttgaaa agcccaatga atgccggaac cggacagaca
      841 atgagtctct ggacaatcgt ggctactttg gaatatgctc atacagaaat atctgagcaa
      901 actggacggg ccaaaatagc cgtctgatcg acttacatta aaggcatgac gggggccatg
      961 ctttaaaaag gaggatcctc atgaaaaata cggggtccaa ttgcattccc atatattgca
     1021 ttggcgatta cggcgagacc gtgaagtcca ctgaattcga tttcgccaga ctggagcgca
     1081 gactagaggt tctgccgtac cctcaccggc atgacttcta ccatatagtt tgggttactc
     1141 acggcgatgg caaccatatc attgattccg tatcgtatcc ggtcaagagc aacgttatat
     1201 ttttcctggc accgcggcaa atacacgacc tggacctgcc cgaagacaca accgggttca
     1261 ctattaactt tagccccgaa ttcttctttt taaatcctac aaaccggaat atatcaagcc
     1321 actcggtatt ctacggagca aacgagcggg tgcaggcgct gtatctgacc gatgagcagg
     1381 ccgaaagcct gaaaaacatc ataaaagaaa tcgagagcga gtactacagc gaatttgcaa
     1441 accgctacga gatcattcga gcgtatctgt acatattttt gacacacgcc gaacgctacg
     1501 cttgccaggc caagcatgct gcaattacgc ctcacagctt tgcgctgaca caaaaattca
     1561 aggctttgct cgaagccgaa catctgtcaa tcggatcggt acaacggtat gcggaaaggc
     1621 ttcatatcac ccctcagcac ctttccgagg cgaccaagat agccacaggc ctgagcgcca
     1681 cgcaattaat gcatcagcat cttgttctag aggccaaacg catgctgggc cattcgaatt
     1741 ccaacgtaac ctggattgcc acacagttgg ggtttgacga tccgggatac ttcagccgtt
     1801 tcttcaagaa acatgtagga caaacgccac gcgagtttca aaagaacgtt cgccccacag
     1861 tcgatcgtcg ccgaaacatg taaaaatcca actaaggaca cgatttgtcc taacggccgg
     1921 tcacgcaata catctattat taacccgctg accggtacat tgaaccatca cgcgcagacc
     1981 tggtgccgtg tttctaaagc tccaaaaagg ccgatatcag cgcaatcaag ccaagctttt
     2041 gacaaagaga aggatatgta cggactggaa ggcatacggg taatcagttt caatcacttt
     2101 cttattggac ccgtgggcgt gcaatatctt gcggatcttg gcgccgatgt gatttccatc
     2161 gaacccatcg acggcgcatt tcagcgcaag tggggcggaa ccggcaaaaa agtggatggg
     2221 cagtcgatgc tgcatttggt gggcaaccgc aataaacgca gcctttctct taatctgaaa
     2281 gacccgcgcg gcatcgaaat cgtgaaacgg ttaatcgcca cagccgacgt gatcaccgag
     2341 aattatcgcc ccggcgttat gaacaaatta gggctgggat acgacgacgc caaggcaatc
     2401 aaacccaata tcatctacgc tgttgcatcg ggtttcggcg cggacggacc ctacgtcaac
     2461 agacccggcc aagatctgat aatacaagcc atgtccggac tggcggcggt caccggcaac
     2521 catgccaatg gcccgcgagc ggttggcgta tcggcggccg accaccacgg ggccgcgctt
     2581 tacgccatgg gcattctagc cgcgttattg cggcgcgaac gcggtggcga aggcggccgc
     2641 gtggacgtta acttgctatc gtcggccatc gatttgcaac aagaatcatt cacctgctac
     2701 ctgaacggcg agcgcccgca ggatattaat cagcctggcc acgttgcggg ctggtgcttc
     2761 ccggcgcctt acggtatcta cgcgagccgc gacggctatc ttgcgatatc ccttgggtcg
     2821 ttgagccttc tttatgacat attgggagtt ccggacacag agcgaatcgc gcaagaggcc
     2881 gaattcaccc agcaaaagac aatatccgaa ctgctggcac ggtatctgat tcaaggcagc
     2941 acggcgcact gggtgcagct attccaggaa aacaaaattt ggcactcgcc agtcaatgat
     3001 tatgaacaag tcatcgagga cccgcaggtc aagcatctcc acagttttaa ttccctgacc
     3061 ggtgcgaccg gcgcgcccat caccctggtc gcgcaccctg tgcgattcga tgggcaaaca
     3121 cccggtgttc ggctcgcacc gcaaaaatta ggggcacaaa ccgtagccat cctgggcgag
     3181 cttggtttca gcggcgatga catcagcaag ttgcacgacg ctggcgtggt caactggtcc
     3241 gacgcctcgg ccgaaacgcc gctccactga aaatcgtttt attaaagaac accatagggt
     3301 gagcagcaca aacgcatatc cgtcctaatc ttcaggagaa tcaaatgtct tcaatgtcga
     3361 tacaaatcgg cgaaaccgtc actttcgaaa aaaccgttgg cgaaaccgac gtgtacatgt
     3421 ttgccgggat tacgggcgat ttttcgggga accatgttag cgaagcctat atgcgaacgt
     3481 ccaagtacgg ccaccgcatc gcgcacggag cgttaatggt gggtttcatg tcgacggcgt
     3541 cgtcggcatt aatcgatcgt tatctggcgc gaggcatgcg ggatacggtt cctgtctcgt
     3601 taggctatga tcgaatacgc tttaccgcac ccgtattctt cggcgatacg atcagggtaa
     3661 cctaccgcgt cgtagaagcg gatgaagagc ggctgcgcac gcgcgccaca atcgaagttg
     3721 ctaaggatga cggcacagtg gttgccgtgg gcgaacattt gatgaaatgg ctcccccgcg
     3781 aaaccactga gcgtggcgat aagtaaccgg aggaaacacc atgcaggaaa actccgcgaa
     3841 ctgcgcaccc gaccctggct cattacgctt gttcgagcag accctggacc gtgcggcgca
     3901 aaaagtggcg gaagacgagt tcacgttggg cgtcgaattt ccacacgtga ccaaccccga
     3961 tggaagctgg gtacgtctgc ccgcatcgct gtcggccggc tacaccgggc aaacgtggag
     4021 ccacggcaac tggctatgcg ggttttgggt ggggctgctg ctggcgtcgt atcttcgaac
     4081 cggcgacaag cactatctgg tctgggcacg cgaacgcatg cgtctggtgg cgcagcgcgc
     4141 cgacgatccc aatacacacg atatcggatt cattttcaat agcagtgcaa tccccggctt
     4201 ccatatcacg ggcgatacct ggtacgcgga cattgccctg caagcggcca acaggctgcg
     4261 cgcaagggtg gtcaccacgc caaacggcgc ctacattgct tcgtggggac ccatgaacga
     4321 cacgcgagga cggcgcagct ccgcgatcga tacgatggcc aatttacccc tgttgtattg
     4381 ggcggcaaac tacagcggcg atggcagctt ccgccttgtg gccgatgcgc acgcagccat
     4441 gacggccagg ggattcatcc ggcccaacca ttccacctat cacgcggtag agtacaacga
     4501 cacaacaggc gagcgtatac ggggctacac gtttcagggc gcattcgacg aatcggactg
     4561 gtcgcgcggt caggcctggg ctatctacgg ctacgttcag tcagcccacg aaacacgcaa
     4621 gcaggaatat ttgcaattgg cggaacagtc ggccaattat tatctcaaga ggcttggcgg
     4681 ccgatccgtc ccgccgtggg atttcgacgc cacaggtgaa gacgccaagg tgaaagatac
//
LOCUS       DATCXU010000289         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_942993,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000289 DATCXU010000000
VERSION     DATCXU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942993"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VIR04_06460"
     CDS             complement(<1..456)
                     /locus_tag="VIR04_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY9460423.1"
                     /translation="METIFNPFGRIGPTKFRNTAIILIAVGAAFSLLPLIRPELTLLS
                     FASLLLFYPWLVIWVKRFHDAGKSGKFFLAVLVVWLAVGFAVNHFIVARFVPAPPPVD
                     PHFVMASIKAQMQANALPGTIASVIISLAFVLILNEELKSEPEENAYGPP"
     gene            complement(518..1357)
                     /locus_tag="VIR04_06465"
     CDS             complement(518..1357)
                     /locus_tag="VIR04_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460424.1"
                     /translation="MIAAYAFFMTFAAQILVVSVLHPIWVINYARVKAEAQLPDFDRD
                     SRDRFFRQYRAINILIAGLGLALLGWMLSQAKGPDWNLQLAVRLLSNFVMAQLAPFCL
                     LSVIAAWVKRKALMNSPPIAKRTATLKRYGLFQIVAPATVALALIAYILFVGAVIYIR
                     HQSIPGFTGYTSLSGITAIYLLNAMSIYWLLYRRKRWPLETSGYRMEAIAEQVKLSFY
                     VGFVAVAFLSLRVVLNLLHLQPWMPFATSVYVVAVMSVSSFMLFALRRQADMDRLNFQ
                     SAV"
     gene            complement(1373..1756)
                     /locus_tag="VIR04_06470"
     CDS             complement(1373..1756)
                     /locus_tag="VIR04_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9460425.1"
                     /translation="MEITIDVDNAMPQFEQLIEQIKAAVASGVLKAGDALPSIRQLAN
                     DLELNSKTVAKAYKLLERDAVIETKGYRGTYVHPKAKTNCKINLQELALRKLSATVAE
                     LREVGITDPEIRNAFAGAISQRIFQ"
     gene            2017..>3215
                     /locus_tag="VIR04_06475"
     CDS             2017..>3215
                     /locus_tag="VIR04_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY9460426.1"
                     /translation="MAGIIRRVCSRAFWTKTKIAFFVLALCAVAAMAFWPSGPKLNYE
                     TAKVVRGDLTVTVSATGTLQPGTKVDVGTEVSGKIDQLLVDSNDHVKKGQLMAVINTD
                     TLQAQLDQFRATLAQARATLATNQATVRQTQAKRARYHTLQDGGAVSALDIQSVDADY
                     ARAIASVSLAKGQIDSAEAQVTNTQTQIGKAKVTAPIDGVVLSRAVSVGQTVQASFSA
                     PVLFTLASDLSVMQLSIDIDEADMGTVKEGQKATFTVDAFPNRKFDARLVTLYNSSKT
                     TNNVVTFPGSLLVDNRSLLLRPGLTATAEILVAEVKNALLVPNAALRFTPPPVGLDNK
                     PPPPAAKAPNGKQGGRVWVLVKNKPEARDLILGHSDGISTEVLSGNLALGDVVLTDIK
                     SQIVRPN"
BASE COUNT          718 a          909 c          946 g          642 t
ORIGIN      
        1 aggtggtccg taggcgttct cttccggttc agatttaagc tcctcattga gaataaggac
       61 gaacgcaagt gaaatgatca cggaagcaat cgtgccggga agcgcgtttg cctgcatctg
      121 ggcctttata ctggccatca cgaagtgggg gtcgacggga ggtggcgccg gaacgaaccg
      181 tgcgacaata aaatggttca cggcaaagcc gacggcgagc cacacgacca gaacggccag
      241 gaagaacttg ccagacttcc cggcatcgtg gaagcgcttc acccagatca ccagccacgg
      301 atagaacagc agcaagcttg cgaaggacag cagggtcaac tccggccgta tcagcggcaa
      361 cagggaaaat gccgcgccga ccgcgatcag gattatcgca gtattgcgga atttcgtcgg
      421 tccaattcgg ccgaatgggt tgaatatcgt ttccatgccg gaatctccgt tgcgtatggt
      481 tggcgaagcg aacttatcgt cggaaaaatt gaaatgctca gacggcggat tgaaagttga
      541 gcctgtccat gtctgcctgg cgccgcagcg caaatagcat gaacgaagac accgacatca
      601 ccgccacgac ataaacactg gtcgcgaatg gcatccatgg ctgcaggtgc agtaaattca
      661 gcacgacgcg gagtgagaga aacgcgacag ccacgaaacc cacataaaag ctgagtttca
      721 cctgctcggc tatggcctcc atgcggtagc cggacgtctc gagtggccag cgcttccggc
      781 gatagaggag ccagtagatg ctcatggcgt tcaggaggta gatggcggtg atgccactga
      841 ggctggtata tcccgtaaat ccaggaatcg actggtgccg gatatagatc acggccccca
      901 cgaacaggat gtaggcaatg agggcgaggg caacggtggc cggggcaacg atctggaata
      961 gcccgtaacg cttcagcgta gccgtgcgct tcgctatcgg cggcgaattc atgagtgctt
     1021 tcctcttcac ccaggccgcg atcacggaaa gaaggcaaaa tggggccagc tgcgccatca
     1081 caaagttgga gagcagcctg actgccagtt gaaggttcca gtccgggcct ttggcttggc
     1141 taagcatcca gcccaacaga gcgagcccca atcctgcgat caagatgttg atggcgcggt
     1201 attgcctgaa aaagcgatct ctcgaatccc ggtcgaaatc cggaagctgt gcctccgcct
     1261 ttacgcgagc gtaattgatc acccaaatcg ggtggagcac cgacacaacc aatatctgcg
     1321 ccgcgaaggt catgaagaat gcatacgctg cgatcatggc gtcacctcaa ttttactgaa
     1381 aaattctctg gctgatggcg ccggcgaagg cgttgcggat ttctgggtcg gtaataccga
     1441 cctcgcgaag ctcggcgacc gtcgcgctca gtttgcgcag cgccaattcc tgcagattga
     1501 tcttgcaatt cgtcttggct ttggggtgca cataggtgcc ccgatagcct ttggtttcaa
     1561 tgacggcatc ccgctccagc agcttgtagg ctttggccac ggttttgctg ttcaactcca
     1621 gatcgttagc caattggcgg attgagggga gtgcatcgcc cgccttgaga acgcccgacg
     1681 cgacggcagc cttgatttgt tcgataagct gctcgaactg cggcatcgca ttgtcgacgt
     1741 cgattgtaat ttccatccgc acgcgttaca tgtaccacat gacccgatgg tacaagtggg
     1801 gcggtgaatt ttcaataggg ggtcaacttg gttgattggt ggcctgatgt ttccgcgtcc
     1861 ggcaacaaaa tcggccggtc attgagatcg ccaagcggtg acaggcgata caaatgcttg
     1921 agcatttcca ggcgcctgac aatcacgccg cggccaatcc ttgtattggt cgatggtgaa
     1981 cattacgtcg atttgggctg aagcagcggg gcattgatgg ctggaatcat tcggcgcgta
     2041 tgttcacgcg ccttttggac aaagaccaag atcgctttct ttgttctggc gctgtgcgcc
     2101 gttgccgcaa tggctttctg gccaagcggc ccaaagctga attacgaaac cgccaaggtg
     2161 gtgcggggcg acctcaccgt cacggtgagc gccaccggca cgctccagcc gggcaccaag
     2221 gtcgacgtcg gtacggaagt atcgggcaaa atcgatcaat tgctggtcga ctccaatgat
     2281 catgtcaaaa agggtcagct gatggcggtc atcaataccg acaccctgca ggcccagttg
     2341 gaccagtttc gtgccaccct ggctcaagcc cgcgccaccc tggcgaccaa tcaagcgaca
     2401 gtgaggcaga cgcaggccaa gcgcgcccgc tatcacactc tgcaggatgg cggcgccgtt
     2461 tccgcgctgg atatccagtc ggttgacgcc gactacgctc gcgccatcgc gagtgtctct
     2521 ttggccaaag gccagatcga cagcgccgaa gcccaggtca ccaacaccca gacccagatc
     2581 ggcaaggcca aggtcacggc gcctatcgac ggtgtggtgc tgtcgcgtgc tgtttcggtg
     2641 ggccagacag tgcaggcatc ctttagcgct ccggtattgt tcacgctcgc cagtgacctg
     2701 tcggtaatgc agctgtcgat cgacattgac gaggccgaca tgggcacggt gaaggaagga
     2761 cagaaggcga ccttcacggt tgacgccttt ccaaaccgca aattcgatgc ccggctggtg
     2821 acgctctata actcctccaa gaccaccaac aatgtcgtca ctttcccggg ttcgctgctg
     2881 gtcgataatc gttcgctgct gctgcgcccc ggtttgaccg ccaccgcgga aatcctggtg
     2941 gctgaagtca agaacgccct tctggtgccc aatgcggcgc tgcggttcac cccgccgccg
     3001 gttggcctgg acaacaagcc gccgccgccg gccgccaagg ctcccaatgg caaacaaggc
     3061 gggcgcgtct gggtgctggt gaagaacaag cctgaagcgc gggacttgat cctgggacac
     3121 agtgacggca tttcgaccga ggtattgtcc ggcaatttgg cgctgggcga tgtcgtgctg
     3181 accgacatca aatcgcagat cgtacgcccc aacaa
//
LOCUS       DATCXU010000290         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_322472,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000290 DATCXU010000000
VERSION     DATCXU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322472"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1427
                     /locus_tag="VIR04_06480"
     CDS             198..1427
                     /locus_tag="VIR04_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEY9460427.1"
                     /translation="MLNTLWLAFFIVASISGLYQWIALGDTEVFSRIVLSLFDMADLS
                     VKLMILLFGTLTLWLGFLRIAEAAGLIERLALLLTPLFRRLMPGVPAGHPALGLITLN
                     FAANALGLDNAATPIGLRAMRELQTLNLNPKTATDAQILFLVLNASSLTLLPVTIFMY
                     RVQQGASDPTLVFLPILIATTASSLAGLLAVAFMQKLKLHDPVVMAWLGGLILGLGSL
                     MAVLATLSSAAISSMSSLMGNLTLFGIIMAFLVAGARKRIAVYECFVEGAKEGFNISK
                     DLLPYLVAMLCAIGVLRASGALDVLLDGIRWAVQAFGLDTRFIDALPTALVKPFSGSA
                     ARAMMIETMQHFGVDSFPALLAATVQGSTETTFYVLAVYFGAVGVQRARHAVGCALIA
                     DLSGVLASIAVCYWFFG"
     gene            1497..>2110
                     /locus_tag="VIR04_06485"
     CDS             1497..>2110
                     /locus_tag="VIR04_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY9460428.1"
                     /translation="MTSTKEVLSKIVQERRGVLVPGAFNALSAKIVAENGFEAVYVTG
                     AGVTNMHMGLPDLSFVGLHEIAEHTSRIRDAVDIPIIVDADTGFGNPVNTYHTVKMLE
                     RAGADAIQIEDQVFPKKCGHFAGKSVIDQKEMEAKIKAAVDARKDDKLLIIGRTDSAA
                     VVGFEAAVERAQRMGEAGADILFVEAVRKQEEMKRLPALLDRPLL"
BASE COUNT          434 a          592 c          598 g          486 t
ORIGIN      
        1 gctccatgcg gcttttttca cgtttcatgg cgcgcctggc gcgaacgccc gtgacaacct
       61 taaaaatgcc tacggtaacc aaaaaagcaa cgataagaat gatggcgttt gccaactctt
      121 gtcccatgcc atcctctcca attctcgact gcgtggataa tactacgtac ctgtagaaac
      181 cgggatcgca acaccccatg ctcaatacgc tttggcttgc cttttttatc gtggcctcga
      241 tatcggggtt gtatcagtgg atcgcgctag gcgacaccga agtgttctct cgcatcgtgc
      301 tcagtttgtt cgatatggcc gatctatcgg taaaactgat gattctgcta tttggcacac
      361 tgaccttgtg gctggggttc ctgcgcattg ccgaggccgc cgggcttata gaacgcctgg
      421 cgttgctgct gacgccgttg tttcgccggc tgatgcccgg cgttccggcc ggccatcccg
      481 cgttgggcct gattacgctg aatttcgccg ccaacgccct cggcctggac aatgccgcaa
      541 cccccatcgg cctgcgcgcc atgcgcgagc tgcaaacatt gaacctcaat cccaagacag
      601 ccaccgacgc gcaaatcctg ttccttgtgc tgaacgcttc gtcccttacg ctattgccgg
      661 tcactatttt catgtaccgg gtgcaacagg gggcatccga ccctaccttg gtatttttgc
      721 ccattctgat agccaccact gcctcgtctt tggcgggctt gcttgcagtc gccttcatgc
      781 agaaactgaa gctgcacgac cccgtggtaa tggcgtggct gggaggcttg atactgggcc
      841 tgggcagcct catggccgtg cttgccacgc tgagcagcgc cgccatcagc tcgatgtctt
      901 cgctgatggg caatctgact ttgtttggca tcatcatggc gttcctggtg gccggggcca
      961 gaaaaagaat tgccgtttat gaatgcttcg tggaaggtgc gaaggaaggc ttcaatattt
     1021 caaaagacct gttgccatac ctggtggcca tgctttgcgc catcggcgta ttgcgggcgt
     1081 ccggcgcact ggacgtgctt ctggatggca tcagatgggc cgtgcaggcg ttcggactgg
     1141 acacgcgttt tatcgacgct ctgccaacgg cgctcgtcaa gcctttttcg ggcagcgcag
     1201 cgcgcgccat gatgattgaa accatgcagc attttggcgt cgacagcttt ccggcgttgc
     1261 ttgcggccac cgtgcaaggc agcaccgaga ccacgtttta cgtactggcg gtttactttg
     1321 gcgccgtcgg cgtgcaacgc gcacgccacg cggtgggctg cgcattgatc gccgacctgt
     1381 ccggggtatt ggcgtcgatc gcggtgtgtt attggttttt cggttaaacc aggcaattga
     1441 tcggcaggta tacttgcgtc gacaaccatc tcgaacctta ttttccagag ctgaccatga
     1501 catcaacaaa agaagtacta agcaaaatag ttcaagaacg aagaggcgtg ctggtgcccg
     1561 gcgcgttcaa tgcactgagc gccaagattg tggcggaaaa cggattcgag gccgtctatg
     1621 tcacgggagc cggcgtaacc aacatgcata tgggtttgcc cgatctgtca ttcgtcggat
     1681 tgcacgaaat cgcggaacac acctcgcgca ttcgggacgc cgtcgatatt cccatcattg
     1741 ttgacgccga cacagggttt gggaaccccg tcaacacgta tcacacggta aaaatgctgg
     1801 aacgggcagg cgccgacgcc atacagatcg aagaccaggt ttttccgaag aagtgcggcc
     1861 actttgccgg gaagtccgtc atcgatcaaa aagaaatgga agccaaaatc aaagccgccg
     1921 tcgacgcgcg aaaagacgac aaattattga tcatcggccg gactgactcg gcagcggtgg
     1981 tgggattcga agccgccgtt gagcgagcgc agcggatggg tgaagcgggg gccgacattc
     2041 tttttgtcga ggcggttcga aaacaagaag agatgaagcg gcttcccgcc ttgctggatc
     2101 gcccgctgct
//
LOCUS       DATCXU010000291         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_873473,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000291 DATCXU010000000
VERSION     DATCXU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873473"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VIR04_06490"
     CDS             complement(<1..438)
                     /locus_tag="VIR04_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY9460429.1"
                     /translation="MTTRSYKRVLLKLSGEALMGDDAFGINRATILRMTEDISDVVAL
                     GVELAIVIGGGNIFRGVAPGAQGMDRATADYMGMMATIMNALALQDALKHHDIDTRVQ
                     SALNIDQVVEPYIRPKALRYLEEGKVVVFAAGTGNPFFTTDTAA"
     gene            complement(481..1356)
                     /gene="tsf"
                     /locus_tag="VIR04_06495"
     CDS             complement(481..1356)
                     /gene="tsf"
                     /locus_tag="VIR04_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY9460430.1"
                     /translation="MAQITASMVKELREKTDAPMMECKKALTEAGGDLVRAQELLRVK
                     LGNKAGKAAARVTAEGLIGLYIAPDGKLGAVIEVNCETDFVAKNDDFIAFVNKLAELA
                     AVKNPADVAALAALPLGDGTVESVRTSLIGKIGENVLVRRFERIATTAQLASYVHGGK
                     IGVLVEYAGPAETGKDLAMHIAATKPRALDASGVSESEIAAERSVAQEKALKSGKPAD
                     IVTKMIEGSVHKFLKEVTLLSQPFVKNDKQSVEQMLKEKGASIKHFVLYVVGEGIEKK
                     SGDFAAEVAAAANAV"
     gene            complement(1436..2185)
                     /gene="rpsB"
                     /locus_tag="VIR04_06500"
     CDS             complement(1436..2185)
                     /gene="rpsB"
                     /locus_tag="VIR04_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY9460431.1"
                     /translation="MSLMREMLEAGVHFGHQTRYWNPKMAPYIFGHRNKIHIINLEQT
                     VIKFQEAVKFVRQIAMRGGTVLFVGTKRAAREYVAAEAARCGMPYVDSRWLGGMMTNF
                     KTVKSSIKRLKEMETQLAEGATEAMSKKEALMFDRELAKLNKAIGGIKNMSALPDALF
                     IIDVGYHKIAVAEARTLGIPVVAVVDTNHSPDGVDHVIPGNDDSAKAIALYTRGMADA
                     VLEGKEQSLNGVVEGVAEGGEEFVEVPSEHE"
     gene            2422..3228
                     /gene="map"
                     /locus_tag="VIR04_06505"
     CDS             2422..3228
                     /gene="map"
                     /locus_tag="VIR04_06505"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY9460432.1"
                     /translation="MSSLVTDPVDLNKMRIACQEAAQVLDFITPHVVAGVSTSELDRL
                     CLDFINNTLKAESATVGYGPPGYPPFPASICTSVNHVICHGIPGDKVLKNGDIMNIDV
                     TIIKDGWFGDTSRMYYIGEPSILARRLTEATYDCMWLGIEQVRPGATLGDVGHAIQKH
                     AEKLGFSVVREYCGHGVGRQFHQDPQVLHYGKPGTGVRLVPGMIFTIEPMINAGRKDI
                     RQLSDGWTVVTRDHSLSAQWEHSLLVTDTGYEVLTVSKGMPPPPAFVGAA"
     gene            3251..>4706
                     /locus_tag="VIR04_06510"
     CDS             3251..>4706
                     /locus_tag="VIR04_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY9460433.1"
                     /translation="MNAPPIASLRESLQQQRQEAIESFRKHLRPQLLLTTLRRITDHV
                     LRGVIKHYPLPAGAALIAVGGYGRGELYPHSDVDVLILLRAPPTAGDTGRLEALVAAL
                     WNLGLDLGHSVRTIAQCQHEAANDITVETALMEARWLAGGKDLFQALKQAMREQLDVQ
                     TFFQAKRAEMQQRHARHQNTPYALEPNCKEAPGGLRDLQVILWLAQAADLGHTWRQIL
                     HAELLTPAEFRSLRRADRAFKRLRIELHLLAGRREDRVLFDLQPGLASIYGFVATKGR
                     RASEILMQRYYWAARVVSQLNGIVIQNIEERLFPSPDAPVEIIDDDFCSVRNRLDIRR
                     EDCFERNPTLLMRAFLTMQQHPQLLGMSARMLRAAWHARRQIGPSFRRDPVNRNLFVQ
                     ILQQPQGIVHALRRMTMLSILPRYLPAFRKIVGQMQHDLFHAYTVDQHILMVIRNLRR
                     FTMPEHAQEYPFASQLMAGFDRHWLLYIAALYHDI"
BASE COUNT         1021 a         1409 c         1220 g         1056 t
ORIGIN      
        1 ggccgccgtg tcggtggtga aaaaaggatt gccggtgccc gccgcaaaca ccaccacttt
       61 gccttcttca aggtaacgca gcgccttggg ccgaatatag ggttcaacca cctgatcaat
      121 attcagcgcc gactgaaccc gtgtgtcgat atcgtgatgt ttgagcgcgt cttgcagggc
      181 cagcgcattc atgatggtgg ccatcattcc catgtagtcg gcagtggctc gatccatgcc
      241 ctgagcgccg ggagcaacgc cgcgaaatat attgccgccg ccaatcacaa tggccagttc
      301 cacacccaaa gcaaccacgt cggagatgtc ttcggtcatg cgcaaaatag tcgcacgatt
      361 gattccgaag gcgtcgtccc ccattagcgc ctcgccggaa agcttgagga gaacacgctt
      421 atacgatctg gtggtcataa aatccaatcc gagtaaacag ccaagcgaag gtgcaggtta
      481 ttaaacagcg tttgccgccg ccgccacttc cgcagcgaag tcgcccgact ttttctcaat
      541 gccctcgccg accacataca ggacgaaatg tttaatcgac gcgcccttct ccttaagcat
      601 ttgctcaacg gactgcttgt cgtttttaac aaaaggctga gacagcaggg tgacttcttt
      661 caagaacttg tgaaccgagc cttcgatcat cttggtgacg atgtctgcgg gcttgcccga
      721 tttaagcgcc ttttcctgcg cgaccgaacg ttcggctgcg atttccgatt ctgatacgcc
      781 gctggcgtca agcgccctgg gcttggtcgc tgcaatatgc attgccaaat ctttgcctgt
      841 ttcagcggga cccgcatatt cgacgagtac gccgattttt ccgccatgga catagctggc
      901 cagttgcgca gtggttgcaa tgcgttcaaa acgacgcacc aggacgttct cgccaatttt
      961 cccgatcagg gacgtgcgaa ccgactcgac cgtgccgtcg cccaggggca acgccgccag
     1021 cgccgccacg tcggccggat ttttaacggc cgccagttcg gccaacttat tgacaaaggc
     1081 gatgaaatca tcgtttttcg cgacgaagtc agtttcgcaa ttcacctcga tcacggcgcc
     1141 cagcttgcca tcgggagcaa tatacaagcc aatcagccct tcggccgtaa cgcgcgccgc
     1201 agccttgcct gccttgtttc ccaatttgac gcgaagcagc tcttgtgccc ggaccaggtc
     1261 gccgcctgct tccgtaagcg ccttcttgca ctccatcatg ggcgcgtcgg ttttctcgcg
     1321 cagttccttg accattgatg cggtaatttg agccactttt ttctccaatt cattaaagca
     1381 tgccccgaca tagccggggc aggtactgaa acatcaagaa cccaggcgca acgaattact
     1441 cgtgttctga cggcacctcg acgaattctt cgccaccttc ggcaacacct tcgaccacgc
     1501 cattcagact ttgttccttt ccttcaagaa ccgcgtcggc catgccgcga gtgtacaagg
     1561 caatagcttt ggccgagtca tcgttgcccg ggatgacgtg atcgacgccg tcgggcgaat
     1621 ggttggtgtc cacaaccgcc accaccggaa tgcccaaggt acgagcttcg gccacggcaa
     1681 ttttatgata gccgacgtca atgatgaaca aggcgtcagg cagggccgac atatttttta
     1741 taccgcctat ggccttgttg agtttggcca gttcgcgatc gaacataagc gcttctttct
     1801 tgctcatggc ttcggtggcg ccttcggcaa gctgcgtctc catttccttc aatcgcttga
     1861 tcgagctttt gaccgtcttg aagttggtca tcatgccgcc taaccaacgg ctatcgacat
     1921 aaggcatgcc gcaacgtgcc gcctcggcag cgacgtattc gcgcgccgcg cgtttggtgc
     1981 cgacaaatag tacggtgccg ccgcgcattg cgatttggcg cacgaacttg accgcttcct
     2041 gaaacttgat aacggtttgt tcaagattga taatgtggat tttgttgcga tggccgaaaa
     2101 tataaggggc cattttcggg ttccaataac gggtttggtg accaaagtgc acacccgctt
     2161 ccaacatttc acgcattaaa gacatcgttt tctccaaggg tttagtcttg taccaagcca
     2221 gtattcgaca agatgaacac cttgttaaac accctatgtc ggccggcacg ctttttttgc
     2281 caaatctctt ttgaagtttg tctgtaagct ccgggccaag cacaaacccg ggcataaact
     2341 tccaaacgat cctggctgct ttcctaaaat tcaggaaagc ctataattct actatctttc
     2401 cccccctcac ttcaagttgc catgagtagt ctcgttacag atcccgttga tttaaacaaa
     2461 atgcgcattg cgtgccagga agcggcacaa gttcttgatt ttattacgcc ccatgtcgtc
     2521 gcaggggtca gcacaagcga gcttgaccgg ctatgcctgg acttcatcaa caacacgcta
     2581 aaggcagaat ccgcaaccgt cggatatggc ccccccgggt atcctccctt ccccgcctcg
     2641 atttgcacgt cggtcaatca cgttatatgc cacggcattc caggcgataa agtcctgaaa
     2701 aatggcgaca tcatgaatat tgatgtcacc atcatcaaag atggctggtt cggcgacacg
     2761 agccgcatgt attacatcgg agagccctcc atattggcca ggcgcctgac cgaggccacc
     2821 tatgactgca tgtggctggg catcgagcag gtgcggcccg gtgcgaccct tggcgacgtc
     2881 ggtcatgcga tccagaagca tgcggaaaaa cttgggttct cagttgtgcg cgaatattgc
     2941 gggcatggcg ttggacggca gttccatcaa gacccgcaag ttttgcatta cggcaagcca
     3001 ggaaccggcg ttaggcttgt gccgggcatg attttcacaa tcgaaccaat gattaatgca
     3061 ggccgcaagg acatccggca attatccgac ggctggacgg tggtcacacg cgaccatagc
     3121 ttgtccgccc aatgggagca cagtcttttg gtgaccgaca ccggctacga ggtgcttacc
     3181 gtttccaagg gaatgccgcc cccgccagcg tttgtgggtg cggcataggc gtccatttcg
     3241 caacaaggcc atgaacgcgc cgccaatagc ctctttacgc gaaagcctgc agcagcagcg
     3301 ccaagaggct attgaaagct ttcgcaagca cctccgaccc caactattgc tgacaacgct
     3361 gcggcgcatc accgatcatg tgttgcgcgg ggtcataaag cattacccct tgcctgccgg
     3421 cgccgccctg atcgccgtgg gcggctatgg gcgcggcgaa ctttatccac attcggacgt
     3481 cgatgtcctc atcctgttgc gcgcacctcc gaccgccggc gacactggcc ggcttgaagc
     3541 gctcgtcgcc gcgctgtgga accttgggct cgacctgggc cacagcgtgc gaaccattgc
     3601 gcagtgccag cacgaagccg ccaacgacat taccgtcgaa accgcgctta tggaggctcg
     3661 ctggctcgcg ggcggcaaag acctgtttca ggcgctgaag caggctatgc gcgaacaact
     3721 ggacgtgcag acattcttcc aggccaaacg cgccgaaatg cagcagcgcc atgcacgcca
     3781 ccaaaacacg ccctatgccc ttgaacccaa ctgcaaagag gccccgggag gtctgcgcga
     3841 tctgcaggtt attttgtggc tggcgcaagc ggccgacctg gggcacacgt ggcgtcagat
     3901 actgcacgcc gaacttttaa caccggccga atttcgctca ttgcgccgcg ccgaccgggc
     3961 cttcaagcgc ctgcgcattg aattgcattt actggcaggc cggcgcgaag accgcgtatt
     4021 gttcgatctg caacccgggc tggcatctat atacggtttc gtggccacaa aaggccggcg
     4081 cgccagcgaa atactgatgc agcgttatta ctgggcggcg cgcgtggtca gccagctcaa
     4141 tgggatagtg atacaaaaca ttgaagaacg cctgtttccc agtcccgacg caccggtgga
     4201 aatcattgac gacgatttct gcagcgtgcg caaccgcctg gatattcggc gcgaagactg
     4261 ctttgagcgc aaccccacat tactaatgcg cgcgttcctc accatgcagc aacacccgca
     4321 attgctggga atgtcggcgc gaatgctacg cgccgcgtgg cacgcgcgtc gacaaatcgg
     4381 cccttcattt cgccgcgatc cggttaatcg aaatttgttc gttcagatat tgcagcaacc
     4441 ccaggggatc gttcacgcat tgcggcgcat gaccatgctc agcatattgc cccgttattt
     4501 gcccgcgttt cgcaaaatcg tgggccaaat gcaacacgat ctgtttcacg cctacacggt
     4561 agaccagcac attctcatgg tcatccgcaa tctgcgccga ttcaccatgc ctgagcatgc
     4621 tcaggaatat ccgttcgcca gtcaactgat ggccggtttc gatcgccact ggctgttgta
     4681 cattgccgcg ctctatcacg acatcg
//
LOCUS       DATCXU010000292         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1077261,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000292 DATCXU010000000
VERSION     DATCXU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077261"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VIR04_06515"
     CDS             <1..540
                     /locus_tag="VIR04_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY9460434.1"
                     /translation="FVIAVFVYRDIGMRQVPEVLIDASKVTIMLMFIIANALLFAHVL
                     TTERIPQMIAEQIVAWDMAAWQFLIVVNILLLIAGAFMEPTGIILIIAPILFPIAMKL
                     GIDPVHLGIIIVVNLEIGMVTPPIGLNLFVTAGITNMSIGQVIRAAMPWLSVLLVFLV
                     ITTYVPQMSLWLPNFLGGF"
     gene            complement(542..1336)
                     /gene="folE2"
                     /locus_tag="VIR04_06520"
     CDS             complement(542..1336)
                     /gene="folE2"
                     /locus_tag="VIR04_06520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY9460435.1"
                     /translation="MDALIDLPIVMPDVQRTPDTRHLAIQRVGVRGARYPMLIATGKE
                     PQATVADWTLTVALPEQQKGTHMSRFMALLEQNRTTAMTPATMRVMVQDMVRLLDAKD
                     GEISVEFPYFINKLAPVSGVSSLLDYHVRWTARLVAGQAEFEMTLQVPVTSLCPCSKA
                     ISKYGAHNQRSNVTVFAALTGDADMNQLIRLIEAEGSCELWGLLKRPDEKYVTEYAYD
                     NPKFVEDLVRDVAARLQAHPGVGRYRVEAENFESIHNHSAYAVIEG"
     gene            1516..>2242
                     /locus_tag="VIR04_06525"
     CDS             1516..>2242
                     /locus_tag="VIR04_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEY9460436.1"
                     /translation="MLTALLIFLTTIALVIWQPKGLGIGWSATMGAVAALLAGVVQPG
                     DIPIVWGIVWNATLTFVAIIIINLILDEAGFFEWAALHVARWGKGSGRKLFIYLVLLG
                     AAVSALFANDGAALILTPIVAAILLALNFSLRATLAFVMAAGFIADTASLPLVISNLV
                     NIVSADYFKIGFGEYAAVMMPVNIAAVVVTLMVLIARFGRDIPANYDLSKLKMPADAI
                     RDRSTFAAAWWVLTALLIGFFGLQ"
BASE COUNT          439 a          629 c          615 g          559 t
ORIGIN      
        1 ttcgtgattg ccgttttcgt gtatcgcgat attggtatgc ggcaagtgcc cgaagtactg
       61 atcgacgcat ccaaagtcac gataatgctc atgttcatta ttgccaatgc cctgttgttc
      121 gcccatgtgc tgacaaccga gcgtattccg caaatgattg ccgagcagat tgtggcgtgg
      181 gacatggcag catggcagtt tctgattgtg gtgaatatcc tgctgctgat cgcgggcgcc
      241 ttcatggaac ccaccggaat tatccttatc attgcgccga tcctgttccc gatagcgatg
      301 aagctgggca ttgatccggt tcacctgggc attatcatcg tggtgaattt ggagatcggc
      361 atggtgacgc cgcctatcgg cttaaatcta ttcgtgaccg ccggcattac caatatgtcg
      421 atcggccagg tcatacgcgc cgccatgccc tggctgtcgg tgttgctggt cttcctggtg
      481 ataacgacct acgtaccgca gatgtcgcta tggctgccca atttcctggg aggtttttaa
      541 gctacccctc aatcaccgcg taagcggaat gattgtggat ggactcgaag ttttcggcct
      601 cgacgcgata acggccgacg cccggatgcg cctgcaagcg agccgcgaca tcgcgcacca
      661 ggtcttcgac aaatttcgga ttgtcgtaag catattccgt cacgtatttt tcgtcggggc
      721 gttttaaaag cccccacaat tcgcacgatc cttccgcttc gatcagtcga atgagctggt
      781 tcatgtccgc atcgccggtc aaggccgcaa aaacggtcac gttcgaacgc tggttatgcg
      841 cgccatactt cgaaattgcc ttggagcaag ggcacagact tgtcaccgga acttgcagcg
      901 tcatttcgaa ttcggcctgc cctgcgacca gccgtgccgt ccagcgcacg tggtagtcca
      961 gcaaactgct tacccccgac accggcgcca gtttattaat gaaatacgga aattcaacgc
     1021 tgatttctcc gtccttggca tcgagcaggc gcaccatgtc ttgcaccatg acgcgcatgg
     1081 tcgcgggtgt catggcggtc gtgcgatttt gctccagcag cgccatgaaa cgcgacatgt
     1141 gcgtgccctt ttgctgctct ggcagcgcga cggtcaacgt ccagtcagcg acggtggcct
     1201 gaggctcttt accggtcgca atcagcatgg ggtaacgggc gccgcgcacg cccacgcgct
     1261 ggatagccaa gtggcgcgta tccggcgtgc gttgcacatc gggcataacg atcggaaggt
     1321 caatcagggc atccatacag gttccttgca tacatttgcg gttcgtcttg ctttaccaag
     1381 caagttctac agcattggat aaccctgcgg cgagtaatgt tcccgaagat taggattact
     1441 tataatgcgc cgagccgtac gacaacgcga accggcacac ccttcgtccg actgaaccaa
     1501 gaccctgtaa acgcaatgct gaccgccctg ttgatttttc tgaccacgat cgcgctggtc
     1561 atatggcaac cgaaagggct gggtattgga tggagcgcca caatgggcgc ggtggccgcg
     1621 cttcttgcgg gggtggtgca gcccggcgat attccaatcg tttggggaat agtatggaat
     1681 gccaccctga cgtttgtcgc catcattatc ataaatctca tccttgacga ggccggtttt
     1741 tttgagtggg cggcattgca cgttgcgcgc tggggcaagg gcagcggacg gaaactgttc
     1801 atttacctcg ttttgctggg cgccgccgta tcggcgctct ttgccaacga tggcgctgcc
     1861 cttatcctga cacccatcgt cgccgccata cttctggcct tgaacttttc gctgcgggca
     1921 acgctggcat tcgtcatggc ggccggcttc attgccgata ccgccagttt gcccctggtt
     1981 atttcaaatt tggtcaatat tgtatcggcc gattatttca agatcggttt tggcgagtat
     2041 gccgccgtga tgatgcctgt caatatcgcg gccgtagtgg tcactttgat ggttttaata
     2101 gcgcgcttcg gccgcgatat ccctgcaaac tatgacctgt cgaaactgaa aatgcccgcc
     2161 gacgccattc gcgaccgaag cactttcgcg gccgcctggt gggtcttgac cgctctattg
     2221 atcggattct tcgggcttca gc
//
LOCUS       DATCXU010000293         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_492202,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000293 DATCXU010000000
VERSION     DATCXU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492202"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..754)
                     /gene="rplC"
                     /locus_tag="VIR04_06530"
     CDS             complement(65..754)
                     /gene="rplC"
                     /locus_tag="VIR04_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY9460437.1"
                     /translation="MSNSTPTPAAHRLGLVGRKVGMTRIFTDDGESIPVTVLDVSNNR
                     VTQVKSPEVDGYAAVQVTYGSRRASRVTKPQAGHYAKAGAEAGSILKEFRLDPAKAAE
                     FTAGAVLAVESVFEAGQKVDVTGTTIGKGFAGTIKRHHFSGQRASHGNSLSHRVPGSI
                     GQAQDPGRIFPGKKMSGHLGDATRTVQNLDLIRIDAERGLLLVRGAVPGHKNADIIVR
                     PAVKARAKKGA"
     gene            complement(842..1678)
                     /locus_tag="VIR04_06535"
     CDS             complement(842..1678)
                     /locus_tag="VIR04_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460438.1"
                     /translation="MSQVKIELRDVYKVFGGNESEALALLRQGMNKTTVQTQLDCNVG
                     LAGVSLLIPAGQVTCVMGLSGSGKSTLVRHLNRLIDPSAGEVLVDGRDILKLDMPALR
                     QLRRHTISMVFQSFGLLPHINVIDNVAFGLRVRGDGRPASRRIALEWLERVGLVEYAK
                     NYPDELSGGMRQRVGLARALAIDAQVLLMDEPFSALDPLIRRDMQEQLLALQQRLCKT
                     IVFVTHDIDEALRLGQRVAILRDGKIEQAGTPDEIKNHPINEYVERFLLRPAMPDLRA
                     PA"
     gene            complement(1675..>2401)
                     /locus_tag="VIR04_06540"
     CDS             complement(1675..>2401)
                     /locus_tag="VIR04_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460439.1"
                     /translation="PPAVLLLLTGLLAWHATRRKALALAYVIGLYAIGAFGLWDKLIQ
                     TFALVFVATVVSIIVGIPIGIATARSRWMQRLLTPVLDVMQTLPSFVYLIPVLMFFGL
                     GKVPALFATIIYAVPPLIRLTALGLRQVDHDIVEAAQAFGVTRWQMLTRVTLPLARPS
                     IMAGINQTTMMALSMVVVASMIGARGLGEDVLAGIQTLDVGQGLQAGVAIVILAIVID
                     RITQAYGRSHRATSKLRRKENEK"
BASE COUNT          520 a          735 c          662 g          484 t
ORIGIN      
        1 gtatcgggcg ccgcgacggt ggatgcggat tgaccctgat cattcaggag cttgagttcc
       61 attattaagc tcctttcttg gcgcgcgcct tgacggcggg gcgcacgatg atgtcggcgt
      121 ttttatggcc cggaacagcg cccctgacca acaacaaccc gcgttccgca tcgatgcgaa
      181 taaggtcgag gttctggact gtgcgggtcg cgtcgcccaa atgacccgac attttcttgc
      241 cgggaaaaat acggcctgga tcctgcgcct gtccaattga acccgggacg cgatgcgaca
      301 gtgagttgcc gtgggaagcg cgctggcccg aaaagtggtg gcgcttgatg gtgccggcaa
      361 agcctttacc aatcgtagtg cccgtaacgt ccactttttg acccgcttcg aacacggatt
      421 cgaccgctag tacggcgccg gccgtgaatt cggcggcctt ggccggatcg agacgaaatt
      481 ctttgagaat gctgccggct tcagcgccgg ccttggcata gtgccctgct tgtggcttgg
      541 tcacgcgcga cgcgcggcga cttccataag taacctgcac tgccgcgtag ccatctactt
      601 ctggcgactt gacctgcgta acgcggttgt tcgacacgtc cagtactgtg accgggatgg
      661 attcgccatc gtcggtgaaa atacgggtca tgccgacttt gcgcccgacc aggcccagcc
      721 gatgggcggc aggcgtaggt gtcgagttcg acatcgtttt tctccattcc cgactacgat
      781 tggtcggggc tattaaataa ttaaccaaac ttttttggct aagccctgca ctttatcacg
      841 gttaagcagg tgcgcgcaag tcgggcattg cgggccgcaa caaaaaccgt tcgacatatt
      901 cattgatcgg atggtttttg atctcgtccg gcgtacccgc ctgctcgatt ttgccatcgc
      961 gcaaaatagc cacgcgttgt cccaggcgca gcgcttcgtc gatgtcgtgg gtaacaaaaa
     1021 caatggtctt gcacaggcgc tgctgcagcg caagcaattg ctcctgcatg tcccggcgaa
     1081 ttaatggatc cagtgcggaa aagggctcgt ccatcagcag cacctgcgcg tcgatagcca
     1141 gcgcacgcgc cagaccgact ctctgacgca tgccgcccga cagctcatcg ggataatttt
     1201 tggcgtactc gacaagtccg acccgttcga gccattccag agcaatacgc cgcgacgcgg
     1261 gcctgccgtc gccccggact cgcaagccaa acgccacgtt atcgatgaca ttgatatgag
     1321 gcaacagacc aaagctctga aacaccatgc tgatcgtatg gcgccgcaac tggcgcaaag
     1381 cgggcatatc cagctttaat atgtcgcgcc catcgaccag gacctcgccg gctgacggat
     1441 caatcaaacg gttcaagtgg cgcaccagcg ttgacttgcc cgatcccgac aatcccatga
     1501 cacacgtgac ctggccggct ggaatgagca ggctgacgcc cgccagaccg acattgcaat
     1561 caagctgcgt ctgaacggtt gttttgttca ttccctgacg cagcagcgcg agcgcttcgg
     1621 attcattgcc gccgaacacc ttgtaaacat cgcgcagctc gattttgacc tggctcattt
     1681 ttcgttctct ttacggcgca acttgctcgt cgcccgatgc gaccggccat aggcctgtgt
     1741 aatccggtca atcacaatcg ccaggataac gatcgccacg cctgcctgca ggccctgccc
     1801 gacatccagg gtctgaatcc ccgccagaac gtcctcgccc agaccacggg cgccgatcat
     1861 ggaggccacc accaccatcg acaaggccat catggttgtc tggttgattc ccgccataat
     1921 gctgggtcgc gccagcggca aggtaacgcg cgtcaacatc tgccagcgcg tcacgccaaa
     1981 cgcctgcgcc gcttccacaa tatcgtgatc cacctgacgc aggcccaggg cggttagccg
     2041 aatcagaggc gggacggcat aaatgatcgt ggcgaataac gccggtactt tgcccagacc
     2101 aaaaaacatc agtacgggga taaggtatac gaagctcggc aaggtttgca tcacgtccag
     2161 caccggggtg agcaaacgct gcatccagcg gctgcgcgcg gttgcaattc cgatgggaat
     2221 gcccacaata attgaaacca ctgtcgctac aaacaccagc gcgaacgtct gtatgagctt
     2281 gtcccacaag ccaaaggcgc cgatggcata caagccaatc acataggcca gcgccagcgc
     2341 tttcctgcgt gtcgcatgcc acgccagcaa accagtcagc aatagcaaga cggcgggagg
     2401 a
//
LOCUS       DATCXU010000294         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_953000,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000294 DATCXU010000000
VERSION     DATCXU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953000"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VIR04_06545"
     CDS             complement(<1..184)
                     /locus_tag="VIR04_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460440.1"
                     /translation="MLEQQLFNALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLG
                     SYVALLLVDKLAVSLPL"
     gene            complement(291..1451)
                     /locus_tag="VIR04_06550"
     CDS             complement(291..1451)
                     /locus_tag="VIR04_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460441.1"
                     /translation="MQIRHLLKALPVLLIGASMASTVYAADIKIGVAEALTGPAGKYG
                     VAIKNGFILAADEVNAKGGVNGNKLKLIIEDEQGKKEDAINAFKKLIFQDKVLMVFGP
                     TLSNSAFAADPVANAAKVVAFGTSNTADGITQMGPYTFRNSVMEADVLPVSTRAAVKH
                     FNLKKVAVIYGNDDAFTKDGYNVFKTTLADQKIPVTDTETYAKGDVDFKAQLTKIKAG
                     NPDAIVCSCLAEEAGNIILQARSLGIKQPFIGGNGLNSPKLFDIAKDAADNSIMGSPW
                     SSENTAPANQAFIKAYQAKFNMPPDQFAAQAYDGMNIAVAALKKVKLTGNLAKDRDAV
                     RVALPDVQWEGATGKFKFRPAPASKTGKAVGYDAQQEAIVNIAKQGKFVVLK"
     gene            complement(1642..2466)
                     /gene="tam"
                     /locus_tag="VIR04_06555"
                     /pseudo
     CDS             complement(1642..2466)
                     /gene="tam"
                     /locus_tag="VIR04_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
     gene            2601..3452
                     /locus_tag="VIR04_06560"
     CDS             2601..3452
                     /locus_tag="VIR04_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460442.1"
                     /translation="MPQRDRDLSKRRNSLNKKSLTTFVAVLIAGSFFSFFGQASLAAG
                     KTSIVVGADTTFPPFETEKNGEVTGFDIDMIKAIAKAENLEIKLKTLPFNGIIPSLQA
                     GSIDVAVAGITIKKSRMQSVDFSNAYYKSGLSVLVKNDSEIKSFADLKGRVVATKKAT
                     SSVDYMKSHGIDSKYIKEFQNIDVAYQALVTGGADAVVFDNPVNVDFMVQNKNVKIVG
                     DLLTGEYYGIAVTKKEPGLVDKINAGLEKIKKTGEYRQLFVKYFGGDTHGEVKGIEKP
                     SDVAITD"
     gene            3561..4229
                     /locus_tag="VIR04_06565"
     CDS             3561..4229
                     /locus_tag="VIR04_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460443.1"
                     /translation="MNVIWQNLPVILDGLGLTIQYSIVAIVLMVIVGLVAALMRLSKS
                     RILRGISTAYVEAFRSTPLLVQLFFIVLGLPALLPVNQWFGQLFYPILAAAVTLGLNE
                     GAYVTEIIRAGILGVDRGQKEAASSIGMTGIQNMRYIVLPQAFKRMIPPLVNQAAQTV
                     KDTSLLAPVGIIELVYKGEVVIAATFASFEIWGLIAVLYFIIIFSVSKCASYLERRLQ
                     VDKR"
     gene            4216..4938
                     /locus_tag="VIR04_06570"
     CDS             4216..4938
                     /locus_tag="VIR04_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9460444.1"
                     /translation="MINVKNLHKSFGHVEVLKGIDSHVDEKEVVVVIGPSGSGKSTFL
                     RCLNGLEEATSGEIVIAGMNLTDPKTNIHKLRQHVGMVFQQFNLFKHLTILENITIGP
                     RKVKKMPLEQSNKIARALLAKVGLAGKESNFPDELSGGQQQRVAIARALAMEPNVMLF
                     DEPTSALDPEMVGEVLQVMKTLAVEGMTMVVVTHEMGFAREVGHRVIFMDEGRIVEEG
                     SPAAIFSGAQMERTKNFLAKIL"
     gene            complement(4974..>5256)
                     /locus_tag="VIR04_06575"
     CDS             complement(4974..>5256)
                     /locus_tag="VIR04_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460445.1"
                     /translation="SEKSMSTVKTQKPYPDAVPPIKIWAHVPEPPDKSPNVPPGVPPP
                     DQPDEVELPPREDPNKIDEPEIPPVPEPPMKAIARRTHTAFNATHRMGP"
BASE COUNT         1166 a         1313 c         1502 g         1275 t
ORIGIN      
        1 caagcggcaa ggataccgcc agtttatcca ccagcagcag agcgacataa ctgcccagca
       61 tgaaaattgc gccgtgagac aggttgatca cgcccaggac accaaagacg agtgtaaaac
      121 cgagcgcaaa aagggcgtat acgctgccaa gcgaaagcgc gttaaagagc tgttgttcga
      181 gcatggttcg ggtaggtaac agtaaaggca ctttcggcaa ggagccgaaa atgcctttac
      241 cttggattta agactattgc cagatgactt tagtcgactg gccgtttaac ttatttaagc
      301 accacaaact ttccctgttt ggcgatgttg acgatggcct cttgctgcgc atcgtagccg
      361 accgccttgc cggtcttgga tgcgggcgcc gggcggaatt tgaacttgcc ggttgcgccc
      421 tcccactgca cgtcgggcag tgccacacgc accgcatcgc gatctttggc gaggttgccg
      481 gtgagtttca ccttcttgag cgcggccacc gcgatattca tgccgtcata agcttgggcg
      541 gcgaactggt cggggggcat attgaatttg gcttgatagg ccttgatgaa tgcctggttg
      601 gcgggagctg tgttttccga ggaccagggg ctgcccatga tgctgttgtc ggccgcgtct
      661 ttggcgatgt cgaataattt tggcgagttc agtccgttgc cgccgataaa cggctgtttt
      721 ataccgagcg aacgcgcctg caggataata ttgcccgctt cctcggcaag gcaggagcaa
      781 acaatggcgt cagggttgcc ggcctttatc ttggtgagtt gcgccttgaa atcaacgtcg
      841 ccctttgcat aggtttcggt atccgttacg ggaatttttt gatccgccag ggtggtcttg
      901 aagacgttat agccgtcttt ggtgaaagcg tcgtcattgc catatatgac ggccactttc
      961 ttaagattga aatgcttgac cgccgcgcgg gtgctgaccg gcaaaacgtc agcttccatt
     1021 accgagttgc ggaatgtgta tgggcccatc tgggttatgc cgtcggccgt gttgctggtt
     1081 ccaaatgcca ctaccttggc ggcattggcg acggggtcgg cggcaaaggc cgagttcgac
     1141 agggtggggc cgaataccat cagcaccttg tcctggaaaa tcagtttttt gaaggcgttg
     1201 atggcgtctt ccttcttgcc ttgttcgtcc tcgatgatca atttcaactt gttgccgtta
     1261 acgccgccct tggcgttgac ttcgtcggcg gccagaataa agccgttctt tatcgcgacg
     1321 ccgtatttgc ccgcggggcc ggtcagcgcc tcggccacgc caatcttgat gtcggcggca
     1381 tagacagtgc tggccatgct cgcgccaatt agcagcacag gtaaagcttt caataaatga
     1441 cggatttgca tgtttttgct ttccatgatg gttggaacaa tcaaagcgca ggcgagttta
     1501 cacagcacta aagccacacg acagcgtgca cggcgctcgt tgcgcctgca ttatgcaccc
     1561 gcatgatggc gctggtcaaa aagtcagggc ggccgccgcg gctttcaggg ccggttcttg
     1621 cgaatgcatg cacggcagcc ctcagttggc ggtcgcaacg atgaaaagtc gcgggaatgg
     1681 caacaacacg gttccatcgg gcagggcggg gtaggctttg gctaccgctg cggtgtagcg
     1741 gtcgagatag gcctttcgtt cgtcatcgtc aagagggccc aggaatgggc gcaagccgct
     1801 gcccttgaac cattcgacca ccgcttgcgc gccgccagca agcggatgat ggtaggtggt
     1861 gcgccacaca tccaccttgg cgcacgcagg gcgcagcgtc cggtaatacc agtttgcgtc
     1921 ttgccttgcg gtttgcacct tcgccgcacc gcccaatttt tctgcccatg ggccatcggc
     1981 cgctatttcg cgcatcagcc ggtgcgcggg ctcctggaga ttgtcaggca tttgaacggc
     2041 aagactgccg cctgcggcca acttggcaag gagcgatgga aacagagcag cgtggtctgg
     2101 cacccagtga agaacggcgt tggccagtat tacgtcgaat gggccggatt cgctccatgc
     2161 tccaatgtcg gttaattcaa attgcacttt cggcagtcgc ttgcgcgcgg cggcgatcat
     2221 gtcgggcgag ctgtccagtc ctcttatcgt cgcatggggg aaccgcttgg cgagaagctc
     2281 tgttgagttg ccgggtccgc agccgatatc aatggccaag tttgcttcag tggccggtac
     2341 agcagcgagc agatcgcgta ccggacgcgt gcgctcatct tcaaagctta cgtattgttt
     2401 ggcagaccag ctcattgtgg ctctctcctg atggcaattg caagttgtag tgtattgccg
     2461 cgaggcaaaa ttgttcctgg cggaccgggt ccgttcgtcc cttcatcttt tgaaaagtta
     2521 ccatatttac cattacgtca tcttgacttc ggaactgggc caataccata ctaggcactg
     2581 caataatcgt tttcttccga ttgcctcaac gcgaccgaga tctttccaaa aggagaaatt
     2641 ctttgaataa gaaatcatta actacgtttg tcgcggttct cattgccggt tccttttttt
     2701 cattctttgg ccaagcctcg ctggcggccg gcaagacctc tatcgtggtt ggagcggaca
     2761 caacatttcc tccgttcgag acggaaaaaa atggagaggt gaccggcttc gatattgaca
     2821 tgatcaaggc catcgccaag gctgaaaacc tggagataaa actaaaaacg ctaccattca
     2881 acggaattat tccgagtctt caggccggtt cgatcgatgt tgcggtggct ggaatcacca
     2941 tcaagaaaag ccggatgcaa agtgtggact tcagcaatgc ctattacaaa tcgggtttgt
     3001 ccgttcttgt taaaaacgat tccgagataa aaagcttcgc ggatttgaaa ggacgtgttg
     3061 ttgccacgaa gaaagcaacc tcgtcggttg actacatgaa aagtcacggc attgattcca
     3121 aatatataaa agagttccag aatattgatg tcgcttatca ggctttggtg acgggcggtg
     3181 cggatgctgt ggtatttgac aatccggtca atgtcgactt catggtgcag aacaaaaatg
     3241 tgaaaatcgt cggcgacctt ttgaccggag agtattacgg tattgctgtt actaaaaagg
     3301 agccaggtct cgttgacaag atcaatgccg gtctggaaaa aatcaaaaaa acaggcgagt
     3361 accgccagtt attcgtgaag tactttggcg gcgacaccca tggcgaggta aaaggaattg
     3421 aaaaaccctc cgacgttgct ataacagact gaccattttc agtttgggat gtgtatcgcg
     3481 cgcagtacgc atgttgtgct gcgcgttttt gatggcgcca ggcaacgcct gcaaccagaa
     3541 cgcacggcaa ataaaacagc atgaatgtaa tttggcagaa tcttcctgta attctggatg
     3601 gccttggttt gacgattcaa tactcgatcg tggccatcgt cctgatggtt attgtgggtc
     3661 tggtcgccgc cctgatgcgg ctgtccaagt cccgtattct caggggaatt tcaacggcct
     3721 atgtggaggc ttttcgttcg acgcccttat tggtgcaact gtttttcatt gtgctggggc
     3781 ttcccgcctt gttgccggtc aaccagtggt tcgggcaatt gttttatccg atcctggctg
     3841 cggcggtgac gctgggtttg aacgaaggcg cgtatgtaac ggaaataatc cgcgcgggaa
     3901 ttcttggcgt cgaccgcggc caaaaggagg ccgcatcaag catcggaatg actggcattc
     3961 agaacatgcg atatatcgtc ctgccgcagg cgtttaaacg catgattccg ccgctggtca
     4021 accaggcggc gcaaacagtc aaagacacat cattactggc gccggttgga atcatcgagc
     4081 ttgtctataa aggcgaggtg gtgatcgcgg cgacctttgc ttcctttgaa atttgggggc
     4141 ttattgcggt actgtatttc atcattatct tctccgtatc gaaatgcgcc tcttacctcg
     4201 agaggaggct tcaagttgat aaacgttaaa aatttacaca agtcttttgg gcatgtcgaa
     4261 gtactgaaag gaattgacag ccacgttgac gagaaagaag tggtggtcgt catcggcccg
     4321 tcaggcagcg gtaaaagcac gttccttcgc tgtcttaacg gccttgaaga agcaacaagc
     4381 ggcgaaatcg taatcgccgg catgaatctg accgatccga aaacaaacat tcacaagctt
     4441 cggcagcatg ttggcatggt gtttcagcaa tttaatctgt tcaagcacct gaccattttg
     4501 gaaaatatta cgattggccc gcgcaaagtg aaaaagatgc cgctggagca gagcaacaag
     4561 attgcgcgtg cgctccttgc taaagtgggc ctggcgggaa aagaaagcaa ctttccggat
     4621 gagttatcgg gtgggcagca gcagcgggtg gccatcgcac gggcgttggc gatggaaccg
     4681 aacgttatgc tgttcgacga accaacgtcc gccctcgacc cggagatggt aggcgaagtc
     4741 ctgcaagtta tgaaaacgct tgcggtagaa ggaatgacga tggtggtcgt tacgcatgaa
     4801 atggggtttg cccgcgaggt gggacaccgg gtgattttta tggacgaggg ccgcatcgtg
     4861 gaagaaggaa gtccggcagc cattttttcg ggcgcgcaaa tggagcgaac aaagaacttc
     4921 ctcgccaaaa ttctgtaagg tcaaaactgg caatgggctt cggagccggt tattcacggc
     4981 cccatccgat gggtagcgtt gaacgcggtg tgcgttcgcc gggcaatggc tttcatgggc
     5041 ggctccggga caggcggaat ttcaggctcg tcaattttat tcgggtcctc gcgcggcggc
     5101 agctccacct catctggctg atccggcggc ggcactcccg gagggacgtt cggactcttg
     5161 tcgggcggtt cgggcacatg cgcccatatt tttatgggcg gcacagcgtc cggatacggc
     5221 ttttgtgtct ttacagtgct catgctcttc tccgaa
//
LOCUS       DATCXU010000295         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_177435,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000295 DATCXU010000000
VERSION     DATCXU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177435"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="leuA"
                     /locus_tag="VIR04_06580"
     CDS             complement(<1..1543)
                     /gene="leuA"
                     /locus_tag="VIR04_06580"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY9460446.1"
                     /translation="MLSTPSDKYRPFVPFVREFSERTWPSKRISHPPIWMSTDLRDGN
                     QSLIEPMSVERKLRLFEQLLKIGFKEIEVGYPSASQTDFDFVRKLVEENRIPDDVAII
                     VLTPSRDDLIRRTVESAAGAKRAIVHLYNACAPAFRKIVFNMSKDEVREIAISGTKLV
                     KAYTSQHPETTWRYEYSPEVFSTTEPEFALEVCNAVADAWQPTPESKIIFNLPATIEA
                     TTPNLYADQIEWMHNNLKNRESIVLSVHPHNDRGTAVAAAEFAVMAGADRIEGCLFGN
                     GERTGNVDLVTLALNLYTQGINPGLDFSDIDEVRRCVEYCNQLPVHPRHPYVGDLVFT
                     AFSGSHQDAIKKGFARQQADALWEVPYLPIDPADLGRNYDAVIRVNSQSGKGGVSYLL
                     EQEHGLVLPRRLQIEFSRAIQRVTDQTGSEVTAAAGYDIFSKEYLEQDQPFKLVRHRI
                     TGDPKANAGHQFIIEIELEENGEIRQLTGKGDGAIAAFVNALKLPIHILDYNEHAIGT
                     GTDTRA"
     gene            complement(1832..2401)
                     /locus_tag="VIR04_06585"
     CDS             complement(1832..2401)
                     /locus_tag="VIR04_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460447.1"
                     /translation="MKQTSLQQPTRQDDALLRSILLKLTAAQKILCTVVIAAVALIWY
                     DLLNRLIAFGQHIDYSGLRVLGVQAIELVKLYNPFFWWAVVALCTLIIAYFLYTFVKN
                     TQRTAHGKFVSTDVISELVDQFSDPANEVLRWVWQDRRNPITVGDLQQLSREMRTGRA
                     HKIALAREHAAAVDEAAVAPRRNDPLQSH"
     gene            2464..>3210
                     /locus_tag="VIR04_06590"
     CDS             2464..>3210
                     /locus_tag="VIR04_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEY9460448.1"
                     /translation="MQSRQSLELRQRQQLKLTPQLQQSIRFLQCSALELETEIADALL
                     ENPLLEREKEYRTDDADAVGDDSPVDMDRWPRWASSARRHDANDESSRPEIGQSDTLQ
                     QYLLRQLHSTRASIRDRALVTLLIDELDPDGYLPTPLHEIQASLPAELSVDVEELQVA
                     LRLLQSFDPVGVGALSLADCLLLQLKQRDAADGGPACGTGVIKCACDIVEHHLGILAS
                     GNLIRLRQALSCSMNTLRSAHGLLLGLEPRP"
BASE COUNT          668 a          859 c          956 g          727 t
ORIGIN      
        1 ccgcgcgggt gtcggtgccc gtgccaatgg cgtgttcgtt atagtccaga atatgaatgg
       61 gaagctttag cgcgttcacg aacgccgcga tggctccgtc gccctttccg gtcaactggc
      121 gtatttcgcc attttcttcg agctcgattt caatgatgaa ttgatgaccg gcgttggctt
      181 tgggatcgcc cgtaatgcga tggcgcacca gcttgaatgg ctggtcctgt tccagatatt
      241 ctttgctgaa aatatcgtag cctgcggcgg ccgtcacttc ggagccggtt tggtcggtaa
      301 cccgttgaat ggcgcggctg aattcgattt gcaggcggcg cggcaaaacc agaccgtgtt
      361 cctgctcgag caaataagac acgcctccct tgcccgactg actgttcacg cgaatgacgg
      421 cgtcgtagtt gcggcccaga tcggccggat caatcggcag gtaggggact tcccacaagg
      481 cgtcggcctg ctggcgcgca aagccctttt tgatggcgtc ctgatgcgag cccgaaaacg
      541 cggtaaacac caggtctccc acgtacgggt ggcgcggatg tacaggtaat tggttgcagt
      601 actcgacgca gcggcgcact tcgtctatgt cggagaagtc cagccccgga ttgattccct
      661 gcgtatacag attaagcgcc agggtaacca gatcgacgtt gcctgtgcgt tcgccgttgc
      721 caaacaggca tccttcgata cgatcggcac cggccattac ggcgaattcg gctgccgcca
      781 cggcggtgcc gcgatcgttg tggggatgga cgctgagcac aatgctttcg cgattcttca
      841 gattattgtg catccattca atttggtccg cgtacagatt gggcgtggtt gcctcaatgg
      901 tggccggcaa attgaaaatg atttttgatt cgggcgttgg ctgccaggcg tcggccaccg
      961 cgttgcacac ttcaagcgcg aattcaggct cggtcgtact gaatacctcg ggcgaatatt
     1021 cgtaacgcca ggtggtttcg ggatgttgcg acgtgtaggc tttgaccagt ttggtgccgc
     1081 tgatggcaat ttccctgact tcgtctttgc tcatgttgaa gacgattttc cggaatgcgg
     1141 gggcgcaggc gttgtacagg tgcacgatgg cgcgctttgc gcccgcggcc gattcgacgg
     1201 tgcggcgaat caggtcgtcg cgcgatggag tcaaaacaat aatggcgaca tcgtcgggaa
     1261 tacggttttc ttcgaccagc ttgcgcacga aatcgaaatc ggtctgcgat gcagaagggt
     1321 agccgacctc gatttcttta aaaccaattt tcagaagctg ctcgaataaa cgcagcttgc
     1381 gttccacact catgggttca atcaaagact ggttgccgtc gcgcaaatcg gtgctcatcc
     1441 agatgggggg atgggaaatc cgcttgctgg gccaggtgcg ttccgagaat tcacgcacga
     1501 atgggacaaa tgggcgatat ttgtcgctgg gggttgaaag catattgcac ctattgagct
     1561 aaatctgcac taagtcgtga tcgccgctac cgtgaacgtg accgtatttt taaaagggta
     1621 gtctggtcag gccggcgatt atcagagggt gtggcgagag gctaccgagc tgccacgcga
     1681 cgcaaggctc ggcaaccggt cggtagaagt agcgaaagcg ccagcgcaaa gcaggaagcg
     1741 cgacccaacg taacgccgac gccgtggcag tcgggttgaa cgtttgaaaa agtagcgtct
     1801 ttaaatccca tccccgtagt cgatcatatt ttcagtgtga ttgcaagggg tcattgcgcc
     1861 gtggcgccac ggccgcctcg tctacagccg ccgcgtgctc gcgggccagc gcgattttat
     1921 gggccctgcc ggtccgcatt tcgcgggata actgttgtaa atcaccaaca gtgatggggt
     1981 tacgacggtc ctgccatacc cagcgcaaga cttcgttggc cggatccgaa aactgatcga
     2041 ccagttcgct gatgacgtcg gtgctaacga atttgccgtg cgccgtacgc tgtgtgtttt
     2101 ttacgaaggt gtacagaaaa taagcaataa taagcgtaca cagcgcaacg accgcccacc
     2161 agaaaaacgg gttatacagt ttgaccagct cgatggcctg taccccaagc acccgcaggc
     2221 ctgaatagtc aatgtgctgg ccaaacgcga tcagccggtt gagcaggtca taccaaatta
     2281 acgcaacggc cgcaatgacc accgtgcata atatcttctg tgccgccgtc agtttaagca
     2341 gaatgcttcg cagcaaggca tcgtcctggc gggtcggttg ttgtagcgag gtttgcttca
     2401 ttggatctct cgagaaaccc cggggcttca ggtcggggaa gatgtcaacg aaatattgta
     2461 cctatgcaat cacgtcagtc tctggagctt cgccaacgtc agcaactaaa gctgacgccg
     2521 caattgcagc aatcgattag gttcctgcaa tgctcggccc tggagctgga aacggaaata
     2581 gccgacgcac ttcttgaaaa ccccttgctt gaacgcgaga aagagtatcg caccgatgac
     2641 gccgatgcgg taggggatga ttcccctgtt gacatggatc gttggccaag atgggcgtcg
     2701 tctgcccgcc ggcacgacgc caatgacgag tcgagccggc cagaaatcgg gcagtccgat
     2761 accttgcaac aatatctgtt gcgccaattg cattccaccc gggcatcgat tcgcgacaga
     2821 gcgcttgtca cgcttttgat cgatgagctt gacccggacg gctatttgcc cacgccgctg
     2881 cacgagatac aggccagctt gccggccgag ctttcagtcg atgtggagga gttgcaggtg
     2941 gctttgcgcc tgctgcagtc ctttgacccg gtgggggtgg gcgcgctttc gctggccgat
     3001 tgtctgctgt tgcagttaaa gcagcgcgat gcggcggatg ggggtcccgc atgcgggacg
     3061 ggcgtaatca agtgtgcctg cgacatagtg gagcatcacc tgggcatcct ggcttcaggc
     3121 aatctgattc gcctgcgtca ggcgctgtcg tgctccatga ataccttgcg atcggcgcac
     3181 ggcttgctgc ttggtctgga accgcgccca
//
LOCUS       DATCXU010000296         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_244919,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000296 DATCXU010000000
VERSION     DATCXU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244919"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VIR04_06595"
     CDS             complement(<1..434)
                     /locus_tag="VIR04_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY9460449.1"
                     /translation="MATTEKKGPIAFKPKKSVSLSGIVAGNTALCTVGRSGNDLHYRG
                     YDIIDIAHTCEFEEIAHLLVHGKLPNKSELKAYKEKLHGLRGLPAQLQNALETLPAAS
                     HPMDVMRTAVSILGCILPEKDDHNLSDARDIADRLMASLGSAL"
     gene            complement(575..1564)
                     /locus_tag="VIR04_06600"
     CDS             complement(575..1564)
                     /locus_tag="VIR04_06600"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY9460450.1"
                     /translation="MSKPAMRVAITGAAGQIGYAILFRIASGEMLGPDQPVILKLLEI
                     PNEKAQKALQGVMMEIDDCAFPLLAGMSAHSDAREAFKDADVAILVGARPRGPGMERK
                     DLLKVNAEIFTVQGKALNESASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
                     LDHNRALSQLAAKSGKPVGDIEKLVVWGNHSPSMYPDFRFATAGGQSLTALIHDDAWN
                     KDVFIPKVGKRGAAIIEARGLSSAASAANAAIDHVHDWILGTNGKWVTMGVPSDGSYG
                     IPEGIIYGVPVTTQNGEYTRVKDLEIDAFSRERMDLTLKELLEERDGVKDLLK"
     gene            1799..2569
                     /locus_tag="VIR04_06605"
     CDS             1799..2569
                     /locus_tag="VIR04_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9460451.1"
                     /translation="MPDKPLTARPLQSDRSSGSAAFSPLYQQIKGLLLQGLDRGEWKP
                     GESIPSEFELAARFQVSQGTVRKAIDELASENLLLRRQGKGTFVATHHEARVRYRFLR
                     LTPDDGRPPVSGSRILECKRVKASAEIAGLLELRPADVVVNLRRLLSFDQVPTILDEI
                     WLPGVVFRGLTAEFLARNREPLYALFESKFGISMVRADEKIKALAATEDQAALLQIAP
                     AAPLLQVERISYTYGDRPVEVRRGLYITDRFHYRNGLN"
     gene            2665..3078
                     /gene="sdhC"
                     /locus_tag="VIR04_06610"
     CDS             2665..3078
                     /gene="sdhC"
                     /locus_tag="VIR04_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY9460452.1"
                     /translation="MSDSASKPRPQFRNLNLLQILGYRLPPAGKSSIFHRISGALLFL
                     CLPLVILPVFALSVTSPTSFAAMQEWSASPVWRIVLLVLIWGYLHHFCAGIRLLTLDL
                     HWCVALQPAQRTARVVFGVSLALTLVFGLKLFGVW"
     gene            3078..3458
                     /gene="sdhD"
                     /locus_tag="VIR04_06615"
     CDS             3078..3458
                     /gene="sdhD"
                     /locus_tag="VIR04_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY9460453.1"
                     /translation="MATERLGTKRLTVGAHYGTKDFIVQRVSAVIMAVYTLGLLISVI
                     FASELNFETWQHRFTFHWGSVPVGQILATVVFVSLVWHAWVGIRDIWMDYIHSAGLRL
                     FFEAASVLWLAGSVVYFVKILWSL"
     gene            3462..>4237
                     /locus_tag="VIR04_06620"
     CDS             3462..>4237
                     /locus_tag="VIR04_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9460454.1"
                     /translation="MAAVKHSLPRRQFDVVVVGAGGSGMRCALQLAQAGLSVAVLSKV
                     FPTRSHTVAAQGGISASLSNMSEDHWLWHMYDTVKGSDWLGDQDAIEFMCREAPNAVY
                     ELEHFGMPFDRNPNGTIYQRPFGGHTANYGEKPVQRACAAADRTGHALLHTLYQRNVA
                     AHTQFFVEWMALDLLRNDAGDVLGVTALEMETGEVYVLEAKVTVLATGGAGRIWAAST
                     NAYINTGDGLGMAARAGVPLQDMEFWQFHPTGVAGAGVLIT"
BASE COUNT          824 a         1131 c         1218 g         1064 t
ORIGIN      
        1 agggccgatc ccagactggc catgagccgg tcggcgatat cgcgcgcatc ggacaggttg
       61 tggtcgtctt tttccggcag gatgcaaccc aggatggaca ccgcagtgcg catgacgtcc
      121 atgggatggc tggctgccgg caatgtttca agagcattct gcaattgtgc gggcaggccg
      181 cgcagaccgt gcagcttttc tttataggct ttgagttccg atttgttggg cagtttgcca
      241 tgcaccagca ggtgtgcgat ttcctcgaat tcgcaggtgt gcgcaatgtc gatgatgtca
      301 taaccacggt agtgcaagtc gttgccgctg cgtccgactg tgcacagcgc tgtgttgccg
      361 gccacgatgc ccgatagcga aactgatttt ttgggcttga atgcaatcgg accttttttt
      421 tctgttgtag ccatatcgaa actcctagac gtttttacag attttgcgtt gaacccatct
      481 tagtacggat ggacccggga attagctttt aaaacaaagg cccgtatggc ggggcctttg
      541 ttttcagcat gacgccgagt gcgagtttat ccgatcactt gagcaggtct tttacaccat
      601 cgcgctcttc gagcaattct ttaagcgtga ggtccatccg ttcgcgcgaa aacgcgtcga
      661 tttcgagatc tttgacgcga gtgtattcgc cgttctgagt cgtgacgggc acgccgtaaa
      721 taataccttc aggaatgcca tacgagccat ccgatgggac gcccatggtg acccatttgc
      781 cattggtacc cagtatccag tcatgtacat gatcgatcgc cgcgttggcc gctgacgccg
      841 cggatgacag gccgcgggcc tcgatgatgg cggcgccgcg cttgcctact ttgggaatga
      901 atacatcttt gttccatgcg tcgtcatgaa taagggcggt caagctctgg ccgcctgctg
      961 tggcaaaacg aaagtccgga tacatcgatg gcgaatgatt gccccatacg accagctttt
     1021 caatatcgcc caccggcttg ccagacttgg cggcaagttg cgacagcgcg cggttgtgat
     1081 ccagccgcag catggcggta aaatttttgg cgggaagatc gggagccgat ttcatggcga
     1141 tgtaggcatt ggtgttggcg ggattgccca ccaccagcac tttgacattg cggcttgctg
     1201 attcgttgag cgccttcccc tggacggtga aaatttcggc attgacctta agcagatctt
     1261 tgcgctccat gccagggccg cgtggacgcg cgcccaccaa tatggccacg tccgcatctt
     1321 taaacgcttc gcgcgcgtcg ctgtgcgcgc tcatgccggc cagcaacggg aacgcgcaat
     1381 cgtcaatttc catcatgacg ccctgcaatg ccttttgcgc cttttcgttc gggatttcga
     1441 gcagcttgag aatgaccggc tgatccgggc cgagcatttc gcccgatgca atgcgaaaca
     1501 aaatagcgta accaatttgg ccggccgcgc cggtaatggc gacacgcatg gcgggtttgg
     1561 acatggacaa tctccggaaa ataatagtaa ataaatcgtg atgggccgct cggacatgtc
     1621 gcttgtatgg gccatgcgag tcaagcaggt gcagtataaa agattagtga gtcttcatgc
     1681 cacggttaat tattttacat cttatataag acataagagt gttagacaga aatgtcccgg
     1741 catgcgaaaa tagcggtcgt tccataactc gggttggccg tacacgtatc catgaatcat
     1801 gccagataag cctcttactg cccgaccttt gcagtccgat cgctcttcgg gtagcgcggc
     1861 gttcagcccg ctctaccagc agatcaaggg gttgttgctt caaggtctgg atcgcggcga
     1921 atggaagcct ggcgaatcca ttcccagcga attcgaattg gcggcgcgtt tccaggtcag
     1981 tcagggaacg gtgcgcaagg caatcgatga actcgcctct gaaaatttgc tgttgcggcg
     2041 tcagggcaag ggcacgtttg tggcgactca ccacgaggcc agggtgcgtt atcgttttct
     2101 gcggctcacc cccgatgatg gccggccgcc ggtgtcgggc agccgcattc tcgagtgcaa
     2161 gcgcgttaag gcatctgccg agattgccgg cctgctcgaa ctgcgcccgg ccgacgttgt
     2221 ggtgaatttg cgccgcttgc tttcgttcga tcaggttcct acgatactcg acgagatctg
     2281 gttgcccggg gtagtgtttc gcggcttgac ggcggaattc ctggcgcgca atcgcgagcc
     2341 gctctacgca ttgtttgagt ccaaatttgg aattagcatg gtgcgcgccg atgaaaaaat
     2401 caaagcgctg gcggctacgg aagatcaggc ggcattgctg caaattgcgc ccgctgcgcc
     2461 attgctccag gtcgagcgca tttcatatac atacggcgac cgccctgtcg aggttcgccg
     2521 ggggctatat ataacggatc ggtttcatta ccgaaacggt ttgaattaac aagcgttttc
     2581 cgctgatttg atactcgacc cctaaatcgg cgaaaatgcg tgtaatgctt tgcaataaat
     2641 tatcgtttta acctcgaggc cgttatgtcc gattccgcgt caaagccgcg tccgcagttt
     2701 cgcaatttga atttgctgca gattctaggc taccgcctgc caccggcagg aaagtcatca
     2761 atattccatc gcatcagcgg cgccttattg ttcctttgtt taccccttgt gatcttgccg
     2821 gttttcgcgc ttagcgttac ttcgccgaca agtttcgcgg ccatgcagga atggtccgcc
     2881 agtcccgtct ggaggatcgt gctgctggtg ctgatctggg gttatctgca tcatttttgt
     2941 gccgggattc gccttttgac tctggacttg cactggtgtg tagccttgca gccggcgcaa
     3001 agaaccgctc gtgtcgtgtt cggcgtaagt ctggcgctta cattggtttt tggtcttaaa
     3061 ttatttgggg tgtggtaatg gcgacagaac gtctcggcac aaagcgtcta acagtaggcg
     3121 cgcactatgg caccaaagat ttcattgttc agcgcgtttc agcggtcatt atggctgtct
     3181 atacgctggg attgcttatc agcgtaatct tcgcatctga attgaatttc gaaacctggc
     3241 agcatcgctt caccttccac tggggcagcg tgcccgtcgg acaaatcctg gcgacggtgg
     3301 tgtttgtgtc gctggtttgg catgcatggg tgggcattcg cgatatctgg atggattaca
     3361 ttcattccgc tggcttgcgc ttgttcttcg aggcggcatc cgtgctgtgg ttggcgggtt
     3421 ccgttgttta tttcgtaaaa attctctgga gtttataagc cgtggctgct gtcaaacatt
     3481 ccttacctcg ccgccagttt gatgtggtgg ttgtaggtgc cggcggatcc ggcatgcgtt
     3541 gcgctttgca attggctcaa gccggcttgt cggtcgcggt gctgtccaaa gtgtttccca
     3601 ctcgttctca tacggtcgcg gctcagggcg gcattagcgc ctcgctgtcc aacatgagcg
     3661 aagaccactg gctctggcat atgtacgaca ctgtcaaggg ctctgattgg ctcggcgacc
     3721 aggacgcaat cgagttcatg tgtcgcgagg cccccaatgc cgtttacgaa ctcgagcatt
     3781 tcggcatgcc cttcgaccgc aatcccaatg gcacgatata ccagcgtccg tttggcggcc
     3841 atacggccaa ctatggcgaa aagccggtgc aacgcgcctg tgccgccgcc gaccgtaccg
     3901 gccacgcttt gttgcatacg ctgtatcagc gcaatgttgc cgctcatacc cagttctttg
     3961 tcgaatggat ggctcttgat ctgctgcgca atgacgctgg cgacgttttg ggcgtgactg
     4021 cgctcgagat ggaaaccggc gaggtctacg tcctcgaagc caaagtaacg gtgctggcca
     4081 ccggcggcgc ggggcgcatt tgggcggctt cgaccaacgc ctacatcaac acgggcgatg
     4141 gcctgggcat ggcggcccgc gccggcgtgc cactgcagga catggaattc tggcagtttc
     4201 accctaccgg cgttgctggc gcaggggtgc tgatcac
//
LOCUS       DATCXU010000297         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1018612,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000297 DATCXU010000000
VERSION     DATCXU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018612"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2163)
                     /locus_tag="VIR04_06625"
     CDS             complement(<1..2163)
                     /locus_tag="VIR04_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY9460455.1"
                     /translation="MSWKVWRRQWLTEPLYRKAREAMPRLSPTEQQAIEAGDVWWDAQ
                     LFSGRPDWQQFQDIPPVSLTTAEQAFMDGPVRELCSMLNDWDISWRRFDLPPEVWEFL
                     KARGFFGMIIPTQYGGLGFSAYAHSEVVRRISVRSVTAGVTVMVPNSLGPGELLMQFG
                     TQTQREYWLPRLAHGQEIPCFGLTSAQAGSDAASMTDTGVVCRQIVDGVERLGIRLNW
                     RKRYITLSPIATVLGLAFKLSDPDHLIGQIDDIGISVALVPTDLPGVVAGRRHLPAMQ
                     AFLNGPIQGADVFVPLDALIGGVERAGQGWPMLMSALAAGRGISLPSLSAAACAFAAH
                     TSGAYARIRTQFGVSIGQFEGVQEKLGRLAANAYLVEAARRFTCAGLDLGHKPAVVSA
                     IMKLHATEHMRQSVNDAMDLHGGKAVIDGPRNYLGGLYRALPVGITVEGANILTRNLI
                     VFGQGAIRAHPYLMQEVAAIGNNDLEAGEDAFDAIIWKHCWHSVKNAFRALGAAWTGG
                     VFAGSPKGAGAAAPYYRRLNRYAAAFALVSEATVFTLGGSLKREEMLSARLGDVLAEL
                     YLLSAVLKRWSSEGQHEPDLPLVKWCAYQSFATIEKRLDEVLQNFPGRALAFALRACT
                     LPPVFRAKAPPDRSTVACAAILLEPSDARARLVGSVWNNCSAPSVEQLERAFELAVDA
                     QPLLDRLKRSGVKHWRMAHAKGAISQAQAQSLEAAESAA"
     gene            complement(2222..>2434)
                     /locus_tag="VIR04_06630"
     CDS             complement(2222..>2434)
                     /locus_tag="VIR04_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEY9460456.1"
                     /translation="RPVSEDALERVVARAFSQRRKMLRGALGEWTGLLDWDALHIVNT
                     ARAEDLTVDQFISLADALFAAGVFNA"
BASE COUNT          541 a          773 c          675 g          445 t
ORIGIN      
        1 cgccgctgat tcggccgctt caagcgattg cgcttgggcc tgcgatatcg ctcccttggc
       61 atgcgccatg cgccagtgtt tcacgccact gcgtttcaat cggtcaagca agggctgcgc
      121 gtcgacagcc aattcaaaag cacgttccag ttgctccaca ctaggggcgc tgcagttgtt
      181 ccacactgag ccgaccaaac gtgcccgggc gtcggacggt tcgagcaata tcgcggcgca
      241 cgcgactgtg ctgcggtcag gcggcgcttt agcgcggaaa accggaggga gcgtgcacgc
      301 gcgcaatgca aaagccagtg cgcgtccagg gaaattctgc aaaacctcgt cgaggcgctt
      361 ttcaatggtt gcaaagcttt gataggcgca ccatttcacc aatggcaaat cgggctcgtg
      421 ctgtccttcg ctgctccagc gcttaaggac ggcggaaagt aaatagagtt cggccagaac
      481 atcgccgagc cgggccgaga gcatttcttc gcgtttcagc gaaccgccca gtgtgaatac
      541 tgtggcctcg gacaccagcg cgaacgcggc ggcgtaccgg ttcaagcgcc ggtaataagg
      601 cgccgccgcg cccgctccct tgggcgagcc cgcaaacacc ccccccgtcc aggccgcacc
      661 caacgccctg aatgcgtttt taacgctatg ccagcaatgt ttccagataa tcgcatcaaa
      721 ggcatcttcg ccggcctcca ggtcgttatt gccgatagcc gccacttcct gcatcaggta
      781 gggatgagcg cgaatggcgc cttggccgaa tacgattaaa ttacgcgtca gaatattggc
      841 cccttcgact gtgatcccga cgggtagcgc ccgatacaat ccgcccagat aattacgcgg
      901 gccgtcaata acagccttgc cgccatgcaa atccatggcg tcattgaccg attgccgcat
      961 atgctcggtg gcatgcagct tcatgattgc cgacaccacc gccggcttgt gaccaagatc
     1021 caggccggcg caggtgaaac ggcgcgcagc ttccaccaga taagcgttgg cggccaggcg
     1081 gccgagcttt tcctgcacgc cttcgaactg tccgatagag acgccgaact gagtgcggat
     1141 gcgcgcgtaa gcgcccgagg tgtgcgccgc gaacgcgcaa gccgccgccg atagcgatgg
     1201 caaggaaata ccgcgcccgg ccgccagagc gctcatcagc attggccagc cctggccggc
     1261 ccgctcaacg ccgccaatga gcgcatcgag aggcacgaag acatcggcgc cctggatggg
     1321 gccgttcaaa aatgcctgca tggcgggcaa gtggcgccgg cccgcgacca caccgggcaa
     1381 gtctgttggc accagcgcca ccgaaatgcc tatatcgtcg atctggccaa ttagatgatc
     1441 cgggtcggat aatttgaatg ccaggcccag cactgtggca atcggactaa gggtgatata
     1501 gcgttttcgc caatttaacc ggatgccgag cctttcgaca ccatcgacaa tctgtctgca
     1561 caccacacca gtatctgtca tcgaagccgc atcggacccc gcttgagcgc tggttaaacc
     1621 gaaacaaggg atttcctgtc cgtgggccag gcgcggcaac cagtattcgc gttgcgtctg
     1681 cgtgccgaac tgcataagca attctcccgg tccaagcgaa ttgggcacca tgaccgtaac
     1741 gccggccgtc accgagcgaa ccgaaatccg ccggacgact tccgaatgcg cgtaggccga
     1801 aaaaccaagc ccaccgtact gcgtcggaat aatcatcccg aaaaaaccgc gcgcttttaa
     1861 aaattcccag acttcaggcg gcaggtcaaa tcggcgccac gagatgtccc aatcattcaa
     1921 cattgagcac aactcgcgaa ccgggccatc cataaaggct tgttcagccg tggtcaggga
     1981 aaccggagga atatcctgaa actgttgcca gtcaggccgg ccggaaaaca actgcgcgtc
     2041 ccaccagaca tcgccggcct cgatggcctg ttgttccgtc ggtgagagcc ggggcatggc
     2101 ctcgcgcgct ttgcgataaa gcggctctgt caaccattgg cgacgccata ccttccagct
     2161 cataacattc ccccgggacg aaacaatata tagcgagcgc ccattatctc tcagttgtat
     2221 gtcaggcgtt aaatacaccc gcggcgaaca aggcgtcagc cagagaaatg aattgatcaa
     2281 ccgtcaggtc ttcagcgcgc gctgtattga caatgtgcaa ggcgtcccaa tccagcagcc
     2341 cggtccactc tcccaaagcc cctcgcagca tttttcggcg ttgcgaaaac gcgcgggcca
     2401 ccacacgctc caaagcgtct tcgctcacgg gccg
//
LOCUS       DATCXU010000298         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_717093,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000298 DATCXU010000000
VERSION     DATCXU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717093"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..2252
                     /gene="rpoD"
                     /locus_tag="VIR04_06635"
     CDS             153..2252
                     /gene="rpoD"
                     /locus_tag="VIR04_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY9460457.1"
                     /translation="MPVSAKPAKTPKAAKPKADAPEVKKEKKSAARAVPSGRRPGRPA
                     KNANNDPGHFGDDDVEVEVIPELKPLPRRGGKRAKADKDVPARNQMTPEEQEARRNRL
                     KVLIKLGKDRGYLTYGEINDHLPDDLVDAEAIDGIISTFSDMGISVYDQAPDAETLLM
                     SDNAPVATSDDDVEDEAEAALTTVDSDFGRTTDPVRMYMREMGTVELLTREGEIEIAK
                     RIEDGLKHMVMAIAACPTTVNEILAHIQRVRDGAAQIDEVVDGLVDANGEEYAGAGVA
                     VDDDENEDGPAGGMSSRQLEHLRATALKKFETIAVWFDKMRKAFETEGYEALNYTQAQ
                     EAILNELIGVRFTAKMVEKLADTLRAQVREVRKLERAVLRTCVDRAGMPRLHFIKSFP
                     GNETNLEWVHGEVAAGHAYSETLERQIPAVQELQQKLIDLQVSVVLPLKDLKDVNKRM
                     ATGEAKARKAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRR
                     GYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRINRQILQETGAEPDPA
                     TLAQKMDMPEDKVRKILKIAKEPISMETPIGDDDDSHLGDFIEDTGTLSPSEWALHGS
                     MRDVVKEVLDSLTPREAKVLRMRFGIEMSTDQTLEEVGKQFDVTRERIRQIEAKALRK
                     LRHPSRADKLKSFLEGQ"
     gene            2334..2906
                     /locus_tag="VIR04_06640"
     CDS             2334..2906
                     /locus_tag="VIR04_06640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY9460458.1"
                     /translation="MSQPLQIAVIVGSLRKDSINRKVARTLIALAPASLAPAILEIGH
                     LPLYNQDYDADSPAVYTEFRRKIAAADAILFVTPEHNRSVPAAMKNALDIGSRPSGKS
                     VWDKKPGAVISASPSLQGGFGANHHLRQSLACLNVFCMQQPEAYLSRANLLFNEAGQV
                     SEPGRDLLQNFINNYASWVGRFQPAAVNRS"
BASE COUNT          771 a          808 c          853 g          594 t
ORIGIN      
        1 ccgccgccaa gccgggtgtg aaaaaggttt cgtctaaaaa agttgcggcc aagaaatccg
       61 ctggcgaaaa gttatcccac gaaaaagaaa caagcaagaa agcggccgcg aaggtagcgg
      121 ccaaaacgcc tgctgcgaaa aaaagcgccg acttgcccgt atcggcgaag ccggcaaaga
      181 cgcctaaggc ggctaaacca aaagccgatg cccccgaggt taaaaaggaa aagaagtcag
      241 ccgcgcgtgc ggtccccagt ggacgtcgtc cgggccgtcc tgcaaaaaat gccaacaatg
      301 accccggcca tttcggtgat gatgacgtcg aagtcgaagt catccccgag ctcaagccat
      361 tgccgcgacg cggcggtaaa cgtgcgaaag ccgacaagga cgttccggcc cgcaaccaaa
      421 tgacgcccga agaacaagag gcgcgtcgaa atcgtttgaa agtgctgatc aagctcggta
      481 aagatcgcgg ctatctgacc tacggagaaa ttaacgacca tctgcctgac gatctggtcg
      541 acgccgaagc aattgacggc atcatcagca cgttcagcga tatggggatc tctgtgtacg
      601 atcaggcccc cgacgctgaa acactgttaa tgagcgataa tgcgccggtc gcaaccagcg
      661 acgacgatgt cgaagacgag gctgaagccg cactgacgac ggtcgattct gatttcgggc
      721 gcaccaccga cccggttcgc atgtatatgc gtgaaatggg tacggtcgaa ctgctgactc
      781 gcgaaggcga gatcgaaatc gccaagcgta ttgaagacgg gctcaagcac atggtcatgg
      841 cgattgccgc ctgcccgacc actgtgaacg agatcctggc gcatattcaa cgcgtgcgcg
      901 atggcgccgc tcaaattgac gaagttgtcg acggcctggt ggacgccaat ggcgaagaat
      961 atgccggggc gggcgtcgcg gttgacgatg acgaaaatga ggacgggcca gccggcggca
     1021 tgagcagtcg ccagttggaa cacctgcgcg cgaccgcgct taaaaaattc gaaactatcg
     1081 ctgtgtggtt cgataaaatg cgcaaggcct tcgaaaccga agggtacgag gcgctgaatt
     1141 acacccaggc gcaagaggct atcctgaacg agctcatcgg ggtgcgcttc accgccaaaa
     1201 tggtcgaaaa gctggccgat actttacgtg cccaggtgcg tgaagtgcgc aagctcgagc
     1261 gcgctgtatt gcgaacatgc gtggaccgcg ccggtatgcc caggctgcac tttatcaagt
     1321 cgtttcccgg caacgaaacg aaccttgagt gggtgcatgg cgaagtcgcc gcagggcatg
     1381 cctattccga aacgctggaa cgccaaatcc ccgcggtgca agagcttcag caaaaattga
     1441 ttgatctgca agtcagtgtt gttctgccat tgaaagacct taaagacgtc aacaaacgca
     1501 tggcaaccgg cgaagccaag gcgcgcaagg caaagcgcga aatgacagaa gccaatctgc
     1561 gtctggtaat ttcgatcgcc aaaaagtaca ccaatcgggg tttgcagttt ctggatttga
     1621 tccaggaagg caacatcggt cttatgaagg cggtggacaa gtttgaatat cggcgggggt
     1681 acaagttttc cacatatgcc acttggtgga tacgccaggc catcacgcgt tcaatcgcgg
     1741 atcaggcccg tacaatccgg attccggttc acatgattga aacgatcaac aaaatgaatc
     1801 gtatcaatcg ccagattctg caagaaaccg gggccgagcc cgacccggcc acattggcgc
     1861 aaaaaatgga catgccggaa gataaagtcc gcaagatcct gaaaatcgcc aaagagccca
     1921 tctcgatgga aacgccgatc ggcgatgacg acgattcgca cttgggcgat tttatcgagg
     1981 ataccggcac cttgtcgcct tccgaatggg ccttgcatgg atcgatgcgt gacgtcgtca
     2041 aggaagttct cgattcgctt acgccgcgcg aggctaaagt gttgcgcatg cgtttcggca
     2101 ttgagatgag cactgaccag acgcttgaag aagtaggcaa gcaatttgac gtaacgcgcg
     2161 aacgcatccg tcagatcgaa gccaaggcgc tgcgcaaact gcgtcacccc agccgggcgg
     2221 ataagctcaa aagcttttta gaaggtcagt aacgcgggtt ttacgacata gccccggtca
     2281 acttggccgg ggctagtttt ttttgattta ttgtcaattt aaagagaggt tttgtgagtc
     2341 agccattgca gatcgccgtt atcgtcggca gccttcgaaa ggattctatt aatcgcaagg
     2401 tcgcacgcac gctgatcgcg ctggcgccgg catcgctggc tccggcgata ctcgagattg
     2461 gtcacctgcc tctctacaat caggattacg acgcagattc tcctgcggtt tacaccgaat
     2521 ttcgccgcaa gatcgctgcc gcggacgcta tattgttcgt cactcccgaa cataaccgtt
     2581 cggttcccgc cgccatgaaa aatgcgctgg atataggttc gcgcccatcc gggaaaagcg
     2641 tatgggacaa gaagccaggc gcagtcatta gcgcgtcgcc ttcgctacag ggcggtttcg
     2701 gggccaatca tcatctgcgg caatcgctgg cgtgtctgaa tgtgttttgc atgcagcagc
     2761 cggaagcgta cctgagccgc gctaatttac tctttaacga agcaggtcag gtttccgagc
     2821 cggggcgcga cttgctgcaa aattttatta acaactacgc atcatgggtt ggccggttcc
     2881 agcccgcggc cgtcaaccgc tcctgaccga aaccgcttat tattcaacaa aggtagacag
     2941 cggaattcgc tcgggattgc cagttttatg aataatacgc cagacgacgc actgctgacc
     3001 gttagtgatt tgatagtcga atttcc
//
LOCUS       DATCXU010000299         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_404304,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000299 DATCXU010000000
VERSION     DATCXU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404304"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VIR04_06645"
     CDS             <1..1452
                     /locus_tag="VIR04_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY9460459.1"
                     /translation="ASVLLAFTALGPVSFAQAKTAQALSNLPDFTSIVAETEGSVVNI
                     RTTEAVPVRSPVGPDSEDPYDMFRWFFGPNFQMPGQPGQPGATPPRRRNTPKSGQKER
                     TVPRGVGSGFIISSDGYVLTNNHVVSDSNGIFVTMTNGKEYKAKVVGTDPRTDIALVK
                     IDAKGLTPLTIGDSNALKKGQWVLAIGSPFGLDSTVTAGIVSAINRDTGDYLPFIQTD
                     VAVNPGNSGGPLINLAGQVVGINSQIISQSGGYMGISLAIPIDEVMRVVEQLKVHGKV
                     TRGRIGVQIGEVTSDVAKAIGLKDAKGAMVSKVDESGPAAKAGVRPGDVILKFDNKAI
                     VHMTDLPRIVGTAKPGTNVPMELWRKGKVVKATVTVGEMTVAEAKPEASKPAAPETTA
                     TDSLGLKVSEVSEELHTKGNFKGGVLVAAVEGPAVAAGINVGDVILTVNDDDVTGPKQ
                     FAKLVADLDKSRAAALLVLRGDQTQWITVTPGK"
     gene            1566..>2725
                     /gene="lepA"
                     /locus_tag="VIR04_06650"
     CDS             1566..>2725
                     /gene="lepA"
                     /locus_tag="VIR04_06650"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY9460460.1"
                     /translation="MDYIRNFSIIAHIDHGKSTLADRLIHRCGGLADREMSSQVLDSM
                     DIERERGITIKAQTASLRYAARDGRTYELNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVIPVLNKIDLASADPEGARQEIEDIIGIDASDAIL
                     ASAKTGLGIDEILESIVTRVPAPTGDPAAPLQALIIDSWFDNYVGVVMLVRVVDGVLK
                     PKDKIRFMASGANHLCEQVGVFTPKSEPTDELSAGEGGFVIAGVKELDDAKVGDTVTH
                     SARPADRALPGFKEIKPQVFAGLYPVESSEYDQLRDSLEKLKLNDSSLMFEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDIITTAPTVVYQIEQRDGSVVMIESP"
BASE COUNT          598 a          729 c          819 g          579 t
ORIGIN      
        1 gctagcgtat tgcttgcttt tacggcgcta ggccccgttt cgttcgctca ggcgaaaact
       61 gcacaggcat taagcaacct tcccgatttc acgagtatcg tcgccgaaac cgaagggtct
      121 gtggttaata ttcgtacgac cgaggcggtt ccggtgcgta gtccggtggg acctgatagc
      181 gaagatcctt acgacatgtt tcgctggttt ttcggaccta attttcaaat gcccgggcag
      241 cccgggcagc ctggagcaac gcccccgcgc cgccgcaata ctccgaaatc gggccaaaag
      301 gaacgcacgg tgccgcgcgg agttggctcg ggtttcatca tctccagcga tggttacgtt
      361 ctgacgaata atcatgttgt ttccgattca aacggcatct ttgtcaccat gacaaacggc
      421 aaagaataca aggccaaagt ggtcggcacc gacccgcgca cggacattgc cctggttaaa
      481 atcgacgcga aaggtctgac acctttaacg atcggcgatt ctaacgcgct gaaaaagggt
      541 caatgggtgc tggcgatcgg atcgccgttt ggcctggatt caaccgtgac cgccggcatt
      601 gtcagcgcga tcaaccgcga caccggcgac tacctgccgt ttattcagac cgacgtggcc
      661 gtgaatccgg gtaattctgg cgggccgctt attaatctgg ccggacaggt ggtcgggatc
      721 aattcgcaaa ttatttcgca aagcggcggc tatatgggaa tttcattagc cattccgatc
      781 gacgaggtaa tgcgggttgt tgagcagctt aaggttcatg gcaaggttac gcgaggccgc
      841 attggcgtac aaatcggcga agtgacctcc gatgtcgcca aagcgatcgg tctgaaagac
      901 gccaaaggcg ccatggtgag caaggtcgac gagagcggtc ctgccgcgaa ggcgggagtt
      961 cggccgggcg acgtgatcct gaaattcgac aacaaggcga ttgttcacat gaccgatttg
     1021 cccagaattg tcgggaccgc caaacccgga accaacgttc ccatggaatt gtggcgcaag
     1081 ggcaaggtgg ttaaggctac tgtgacggtg ggcgagatga cggtggcgga agccaaaccc
     1141 gaggccagca agccagccgc tcccgaaaca acggcgacgg actccttggg acttaaggtg
     1201 tcggaggtgt ccgaagaact ccataccaag ggaaacttca agggaggcgt gctggtggcg
     1261 gccgtcgagg gacccgcggt cgcggcaggc attaatgtgg gcgacgtaat cctgacggtt
     1321 aatgacgatg atgtcacggg acccaagcag tttgccaaac tggtcgcgga ccttgataaa
     1381 tcgcgcgcgg cggcattatt ggttttgcgt ggcgaccaga ctcaatggat aactgttacg
     1441 cccggaaaat aatcctcaga aacagctaaa atggcgagat ggccgcaagg gggcgctcta
     1501 tgcgctccct ttttttctcg gctcccgtgt ttatcgctcc aggaggcgaa ttttcccgca
     1561 ggtttatgga ttacatccgc aacttttcta tcattgccca cattgatcac ggcaagtcca
     1621 cgcttgccga tcgtctgatt caccgctgcg gagggctggc cgaccgcgaa atgtcgtccc
     1681 aagtgctgga ttccatggat atcgagcgcg agcgcggcat tacgatcaag gcgcaaacgg
     1741 cgtctttgcg ctacgcggcg cgggacggcc ggacatacga gttgaatctt atcgacacgc
     1801 cgggacacgt cgacttctcg tacgaagtca gccgttcctt gtctgcctgc gaaggcgcct
     1861 tgctggtggt cgacgccagc cagggggtcg aggcgcaaac ggtggcgaac tgctataccg
     1921 cgatcgagct tggcgttgag gtgattccgg ttttaaacaa gatcgacctg gcgtcagccg
     1981 atcccgaagg ggctcgccag gaaatcgagg atatcatcgg aattgacgca tccgacgcaa
     2041 ttctggccag cgccaagacc ggactgggca tagacgagat ccttgaatcg atcgtgaccc
     2101 gcgttccggc tcccacgggc gaccctgcgg cccctttgca ggcgctgatt attgactcgt
     2161 ggttcgataa ttacgtgggc gtggtcatgc tggtgcgcgt cgtcgatggc gtgctcaagc
     2221 ccaaggacaa aatacggttc atggccagcg gggccaatca tttgtgcgag caagtcggtg
     2281 tgttcacgcc caaatctgag ccgaccgacg agctatcggc cggcgaaggt ggctttgtca
     2341 ttgcgggtgt caaagagctt gacgatgcca aagtgggcga cacggtcacg cattcggcgc
     2401 gccccgccga cagggcgctg ccgggtttca aagagatcaa gccgcaggta tttgcaggcc
     2461 tctatcccgt tgaaagcagc gaatacgacc aactccgcga ttcgcttgaa aagctcaaac
     2521 tgaacgattc ttcgcttatg ttcgagcccg aagtttcgca agctctgggt tttggctttc
     2581 gttgcgggtt tttgggtttg ttgcacatgg aaatcgtgca agagcgcctt gaacgtgaat
     2641 tcgacatgga tataataacc accgctccga cggtggttta tcagattgag cagcgggacg
     2701 gctcggtcgt catgatcgaa agccc
//
LOCUS       DATCXU010000300         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_426913,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000300 DATCXU010000000
VERSION     DATCXU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426913"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /gene="ppsA"
                     /locus_tag="VIR04_06655"
     CDS             <1..2112
                     /gene="ppsA"
                     /locus_tag="VIR04_06655"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY9460461.1"
                     /translation="IRQWIVQTPFPSDFERAVRTAFAKLDVDGKGCFAVRSSATAEDL
                     PDASFAGQQETFLNVVGIDDVLDKIRHVFASLYNDRAISYRVHKGFEHAEVALSAGIQ
                     RMVRSDRGSAGVMFTLDTESGFTDVVFITSSYGLGETVVQGAVNPDEFHVFKPTLASG
                     HYPIISRRIGSKLIKMEFDPDRAAGLAVRTVDVPVSERNRYSLTDEEVIELARYAVII
                     EQHYKRPMDIEWGRDGVDGKIYILQARPETVRSQQNQHDVQERYRLKATGEVLITGRA
                     IGQKIGSGKVRVVADISQMDQVEKGDILVTDMTDPNWEPIMKLASAIVTNRGGRTCHA
                     AIIARELGIPAVVGCASATDVLDDGQEVTVSCAEGDEGRIYEGLIETEIEEVRWGAMP
                     DIGLKVMMNVGNPQLAFDFSQIPNEGVGLARLEFIINNNINIHPKAVLDYPNVDGDLK
                     KAVESAARGYASPRAFFVEKLSEGIATIAAAFYPKPVIVRLSDFKSNEYRKLVGGSRY
                     EPDEENPMLGFRGASRYISAEFEDCFRMECQALKRVRDEMGLTNVEIMVPFVRTVPQA
                     AKVVKLLARHGLVRGEGGLRLIMMCEVPSNAILADEFLKYFDGFSIGSNDMTQLMLGL
                     DRDSGMELLAADFDERDAAVKFMLSRAINACLKAGKYVGICGQGPSDHPDLAQWLRDE
                     GILSMSLNPDTVVDTWQRLAK"
     gene            2137..2808
                     /locus_tag="VIR04_06660"
     CDS             2137..2808
                     /locus_tag="VIR04_06660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY9460462.1"
                     /translation="MIFYAQVQSTLGPILLSTDGSSLTGLYFVGQRDCPVLDGLPQPT
                     PEARHPAFGMMDGVPIRKFKAYKRESGQLFQHSAKAAALLAERGKLPASDCITLMQGD
                     TPSSALALFNQADKELREYFAGELRVFKTPLRPAGTPFQKQVWDALLRIPYGEVVSYG
                     DVARVAGLSARHSRPVGSAVGSNPITILIPCHRVVSSTRSLTGYGGGLERKYALLELE
                     GFVLH"
     gene            2823..3251
                     /locus_tag="VIR04_06665"
     CDS             2823..3251
                     /locus_tag="VIR04_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY9460463.1"
                     /translation="MWFWFGFAVLALIGEAVTGTFYLLLFALGLAAGGVAALLQFSLS
                     GQIVAVALVAFVGLMVLRKTGVLKKAEVNAARNRDVNLDIGQLVEVESWSDDDTAQVW
                     YRGANWQARLLKGAAKQAGSQRIIEIQGSTLMVEPSIRKQ"
     gene            3295..4227
                     /locus_tag="VIR04_06670"
     CDS             3295..4227
                     /locus_tag="VIR04_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEY9460464.1"
                     /translation="MDVELSTTVLIVLVLLVFVVVVKAIAIVPQQNAWVIERLGKFDR
                     ALSPGAGFVIPFVERVAYKHSLKEIPLDVPSQVCITSDNTQLQVDGVLYFQVTDPMRA
                     SYGSSDYILAITQLSQTTLRSVIGKMELDRTFEERDVINSTIVASLDEAALNWGVKVL
                     RYEIKDLTPPAEILRAMQAQITAEREKRALIAASEGRRQEQINIATGEREAFIARSEG
                     EKQAQINKAEGEATAVLTVAEATAKAITQVAESVRQPGGMDAVNLKVAERYIEAFGNV
                     AKQGNTLILPANLADMGGLIATAMSVVKNTGKTA"
BASE COUNT          947 a         1173 c         1324 g          968 t
ORIGIN      
        1 attcgccagt ggatcgtcca gacgcctttt ccttccgatt ttgagcgggc tgttcgcact
       61 gcattcgcga aactcgatgt ggacggcaag ggttgttttg ccgtccggtc ttcagcaacc
      121 gccgaggacc tgcccgatgc atcctttgca ggccagcaag aaacgttttt aaacgttgtc
      181 ggcattgacg acgtcctgga taaaatccgt cacgtttttg cctcgcttta taacgatcgg
      241 gcgatttcgt atcgggtcca caagggtttt gaacatgccg aagtcgcact atcggcgggc
      301 attcagcgca tggtgcgttc cgatcgcggc agcgcggggg tcatgttcac gctggacacc
      361 gagtcgggct tcaccgatgt cgttttcatc acttcctcct acggtctggg cgaaacagtc
      421 gttcagggcg cggtcaatcc cgacgagttc cacgtattca agccgacact ggcctcggga
      481 cattacccga tcatcagccg gcgcatcggc tccaagctta tcaagatgga attcgatcct
      541 gaccgcgccg ccggcctggc ggtgcgcacg gtggacgtgc ctgtgtctga acgcaatcgt
      601 tattcgctga ccgacgaaga agtaatcgaa ctggcgcgtt atgcggtgat catagagcag
      661 cactacaagc gccccatgga tattgagtgg ggccgcgacg gcgtcgatgg caaaatctat
      721 atcctgcaag cgcgccccga aacggtccgg tcgcagcaaa accagcatga cgttcaggaa
      781 cgttatcgcc tgaaggccac tggcgaggtg ctgatcaccg gccgtgccat cgggcaaaag
      841 ataggctcgg ggaaggtcag ggtggtcgcc gacatctcac agatggatca ggttgaaaag
      901 ggcgacattc tagtgaccga tatgaccgac cctaactggg aaccgatcat gaagctggcc
      961 tccgccatcg tcacaaaccg cggagggcgg acctgccacg ccgctatcat tgcccgcgaa
     1021 ctgggcatac cggccgtcgt tggctgcgcc agcgccaccg atgttctgga cgacggacag
     1081 gaagtaaccg tgtcatgcgc cgagggcgat gaaggccgca tctacgaagg cctgatcgaa
     1141 accgagatcg aagaagtccg atggggcgca atgcccgaca tcgggcttaa agtcatgatg
     1201 aacgtgggca acccgcagtt ggcctttgat ttttcccaga tacccaacga aggggtgggc
     1261 ctggcaaggc tcgaatttat tatcaacaac aatatcaaca tccatcccaa ggcggttctt
     1321 gattacccca acgtcgacgg cgacctgaaa aaggcggtcg aatcggcagc gcgcggctac
     1381 gccagtccgc gcgccttctt cgttgaaaag ctgtccgaag gcattgccac catcgccgcc
     1441 gctttttatc ctaaacccgt aattgtgcgg ctttccgatt tcaagtccaa tgaatatcgc
     1501 aagcttgtgg gcggctcgcg ttatgaaccg gacgaagaaa accccatgct cgggttcagg
     1561 ggcgcctcgc gctatatttc ggctgagttt gaagactgct ttcgcatgga atgccaggcg
     1621 ctcaagcgtg tgcgtgacga aatgggcctg accaatgttg aaatcatggt tccgttcgtt
     1681 cgcactgtgc cgcaagccgc caaggtcgtc aaactgctcg ccaggcacgg attggttcgt
     1741 ggcgaaggcg gtttgcgcct gatcatgatg tgcgaggttc cttcaaacgc tattctggcc
     1801 gatgagttct tgaaatattt tgacgggttt tcaatcggct ccaacgacat gacgcaactg
     1861 atgctcggtc tggatcgcga ttcaggcatg gaactgctgg ccgccgattt cgacgagcgc
     1921 gacgcggcgg tcaagttcat gcttagccgc gcgatcaacg cgtgtctgaa agcgggtaaa
     1981 tacgtgggca tttgcggcca gggccccagc gatcacccgg acttggctca gtggttgcgc
     2041 gacgaaggta ttttgtcgat gtcgcttaac cctgacacgg tggtcgatac ctggcagcgt
     2101 cttgcaaaat aaagccggat attcgggaaa aagcccatga ttttttatgc gcaagtgcag
     2161 tcgactttgg ggccaattct gctttccact gacgggtcga gcctgacggg cctatatttt
     2221 gtcgggcaaa gggattgccc cgtcctggat ggcttgccgc aacccacgcc cgaggcgcgc
     2281 catccggcgt ttggaatgat ggatggcgtg ccgatcagaa agttcaaggc atacaagcgc
     2341 gaaagcggcc aattgtttca gcattccgcc aaagcagcgg ccctgcttgc cgaacgtgga
     2401 aagctgcccg cgtcggattg cataacgctt atgcagggcg acacgccctc gtcggcgttg
     2461 gcgttgttta atcaggccga taaagagttg cgtgaatatt tcgcaggtga actgcgtgtt
     2521 ttcaaaactc cactgcgacc cgccggaacg ccgtttcaga aacaggtttg ggatgcctta
     2581 ttgcgaattc cctacgggga agtcgtttca tatggcgacg tcgcccgcgt agcgggtttg
     2641 tcggcccggc actcccggcc ggtaggctcg gccgtgggaa gcaacccgat tacaatcctt
     2701 atcccgtgcc accgggtcgt atccagcacg aggtcgctga ccggttatgg cggcggtctc
     2761 gagcgcaagt acgcgctgct tgagctggaa ggctttgttt tacattagcg gagggcagtg
     2821 cgatgtggtt ctggttcggg ttcgcggtgt tggcgcttat aggcgaggcg gtgactggca
     2881 cattttattt gttgctgttc gcgctgggtc tggctgccgg cggtgtcgcc gccttgttgc
     2941 agttttcctt atcggggcag atcgttgcgg tggctctcgt tgcctttgtg ggactgatgg
     3001 tattgcgtaa aacaggggtg ctcaagaagg ccgaagtcaa tgccgcacgc aatcgggacg
     3061 tcaatcttga cataggacaa ctcgtcgaag ttgaatcatg gagcgacgat gacaccgctc
     3121 aagtctggta tcgcggtgcg aactggcagg cgcgtttgct aaagggcgcg gcaaagcaag
     3181 ccggatcgca gcgcatcatt gaaattcaag ggtctacgct aatggtcgag ccttcaattc
     3241 gaaaacaata aacgcgaccg cttgtgcggc gcgtcacttt ctaaggcggc aaatatggat
     3301 gtcgaactct cgaccacggt tttgattgtg ctggtgcttc tggtgtttgt cgtggtggtc
     3361 aaggcgattg caatcgtccc ccagcaaaac gcctgggtga tcgagcgtct gggaaaattt
     3421 gaccgggctt tgtcgcctgg cgccggtttt gtgattcctt ttgtggaacg cgtggcctac
     3481 aagcattcgc tgaaagagat tcctcttgat gtgcccagtc aggtttgcat cactagcgat
     3541 aatacgcagt tgcaggtcga tggcgtgctg tatttccagg tgacggatcc aatgcgggca
     3601 tcgtacggct cgtcggacta tattctggca ataacgcaat tgtcgcaaac cacattgcga
     3661 tcagtcatcg gcaaaatgga gcttgatcgc actttcgagg agcgcgatgt catcaacagc
     3721 acgattgtgg cttcgctgga cgaagcggcg ttaaattggg gcgtaaaagt gctccgttac
     3781 gagatcaagg acctgacgcc tccggccgag attttgcgcg ccatgcaggc gcagattaca
     3841 gcagaacgtg aaaagcgggc gctgattgcg gcatctgaag ggcgccgtca ggagcaaatc
     3901 aacatagcaa caggcgaacg ggaagcgttt atcgcgcgtt ccgaaggcga aaaacaggcg
     3961 cagatcaaca aggccgaagg cgaagccact gccgttctga cggttgccga agcgactgcc
     4021 aaggccatca ctcaagtggc ggagtcggtc cgtcaaccgg gcggaatgga cgctgtgaat
     4081 ttgaaggttg ccgagcgcta tatcgaggca tttggcaatg ttgcaaagca aggcaacacg
     4141 ctgatattgc cggccaacct ggctgacatg ggcggcctga tcgccaccgc gatgtcggtg
     4201 gtgaaaaaca caggcaaaac ggcctgagcg gccttaacgc ctcctccata cgatcgccat
     4261 ccacgccttg cctggatggg aaggcgatac gtatcgcgcg ggtgttcaaa ttccaggcaa
     4321 acacgttccc taacatgttt aagctcgaaa tccgcgcaat ctgatgctcg gcgaggggag
     4381 caggctgggg gagtcggagt aaggcgaagc cg
//
LOCUS       DATCXU010000301         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_604429,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000301 DATCXU010000000
VERSION     DATCXU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604429"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2706)
                     /gene="acnA"
                     /locus_tag="VIR04_06675"
     CDS             complement(4..2706)
                     /gene="acnA"
                     /locus_tag="VIR04_06675"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY9460465.1"
                     /translation="MLHNKFNSLQKFKIGRKTAQLYSLPALGKTLGVDIQRLPVSIRV
                     VLESVLRNCDDKKITEKHVKQLAGWKPKAKRTAEIPFVVARVILQDFTGVPLLVDIAA
                     MRSVADRVSRDPKLIEPMVPVDLVVDHSVMIDHYGTKSALGLNMKLEFQRNQERYQFI
                     KWGKQAFDTFGVVPPGFGIVHQINLEHLARGVHCDAANKLYYPDTLVGTDSHTTMING
                     IGVIGWGVGGIEAEAGMLGQPVYILTPDVVGVELKGELRGGVTATDLVLTITEMLRKE
                     KVVGSFVEFCGPGTSSLSVTDRATIGNMSPEYGATIGFFPVDDRTIEYFKGTGRSKDE
                     VDALKAYFKAQGMYGIPDAKDIAYTKLVTLDLSTVTPSLAGPKRPQDRIEMADIKSTF
                     TRLFSEKSANGFSQPPERLNQVFKTGAGAEVRNGDILIAAITSCTNTSNPSVMLAAGL
                     VAKKAVEAGLKIPKRIKTSLAPGSRIVTEYLVKTGLLPYLEKLGFNLAAYGCTTCIGN
                     SGDLTPDFNEAINKNNLVCSAVLSGNRNFEARIHQNIKANFLASPPLIVAYALTGTIL
                     RDFMTEPVGKGKNGDVWLGDLWPTNAEVKALLGEALDPEAFHKNYDQIETNPGTLWKK
                     IEAIKGNVYNWPESTYIAEPPFFDDFTMQPAPMPPVHSARALAVFGNSVTTDHISPAG
                     AIKEDSPAGAWLKANGVAKHDFNSYGARRGNHEVMMRGTFANVRIKNMMVPANPDGSP
                     FEGGETLYQPTGERLSIYDAAMKYIDAGVPTVIFAGEEYGTGSSRDWAAKGTQLLGVK
                     AVVARSFERIHRSNLVGMGVLPLQFKNNDTAQSLGITGEETYDISGLEKRIKPQQDVT
                     LTIHGKDGSTRQVSLLLRIDTATEVDYYQHGGILPYVLRELLAA"
     gene            complement(2912..3205)
                     /locus_tag="VIR04_06680"
     CDS             complement(2912..3205)
                     /locus_tag="VIR04_06680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY9460466.1"
                     /translation="MNASRPASRAIRHNPERHRFECIEEGHTCVLDYTLRGKQMTITY
                     TGVPAAVGGRGIAAELTQYALNTAQANGWMVHPVCSYAVAYIRKHPEYQSLTV"
     gene            complement(3283..3675)
                     /locus_tag="VIR04_06685"
     CDS             complement(3283..3675)
                     /locus_tag="VIR04_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460467.1"
                     /translation="MFDTFLLYALPLAFTILVICALTSLVSGLDLLLFKVRKTNELLR
                     HPYLRQQPFDRYPVAIRAAILMDYFFRLSFPGSQFWIIGQANRLLGHIDPKEIPTTIK
                     WPLVGLWGGCFVGLIAMLAVWTLLLFRL"
     gene            complement(3761..>4834)
                     /locus_tag="VIR04_06690"
     CDS             complement(3761..>4834)
                     /locus_tag="VIR04_06690"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEY9460468.1"
                     /translation="LSRTAVAAGKAPLTMRDVLTGMIKAHEIQGCIALENSFNKVGLD
                     HVLLVKVASTAVVSQMLGLTRAETINAISLAWVDGQSLRTYRHAPNAGSRKSWAAGDA
                     TSRAVRLALIARTGEMGYPSVLTAKTWGFYDVSFKGQPFKFQRPYSSYVMENVLFKIS
                     YPAEFHSQTAVECAMDIHQKLQAAGKTPDDIKSITIRTHEACIRIIDKSGPLNNPADR
                     DHCIQYMVAVPIIFGRLTAGDYEDSIAADPRIDALRAKIVCREDPAFTADYHNPEKRS
                     IPNALTVEFNDGSRLDEIVCEYPIGHKRRRQEGIPLLENKFRINLARRFPARQQRRIL
                     DVSLNQQALEAMPVHEYVDLYVI"
BASE COUNT          967 a         1274 c         1442 g         1151 t
ORIGIN      
        1 tcgttacgcg gcgagcagct cccgcaacac gtacggcaag ataccgccgt gctggtaata
       61 atcgacttca gtggcagtgt cgatacgcag aagcaggctt acctgccgtg tagatccatc
      121 tttgccgtgt atggtcagcg tgacatcttg ttgcggcttg atgcgttttt caagacccga
      181 gatgtcgtag gtttcctcgc cggtaatacc cagcgattgc gccgtgtcat tgtttttgaa
      241 ctgtagcggc aatacaccca tgcccaccag gttgctgcgg tggatgcgct cgaagcttct
      301 tgccacgacc gccttgacgc ccagcaattg ggtgcctttg gcggcccagt cgcgtgacga
      361 accggtgccg tattcttcgc cggcaaaaat aacggtcggt acgccggcgt cgatgtactt
      421 catggccgcg tcgtaaatgg acaggcgttc accggtgggt tgatacaagg tttcgccgcc
      481 ttcgaaggga ctgccgtcgg ggttggccgg gaccatcatg ttcttgatgc gaacgttggc
      541 aaaggtgccg cgcatcatga cttcgtggtt gccccggcgc gcgccgtagc tgttgaaatc
      601 gtgtttcgct acgccgtttg ctttcagcca tgctcctgcg ggcgagtctt ccttgatggc
      661 gccggctggc gagatgtggt ccgtggtgac ggaattgcca aagacagcca gggcccgggc
      721 ggaatgcacg ggcggcattg gagccggctg catcgtaaag tcatcgaaga acggtggttc
      781 cgcaatatac gtggattccg gccagttgta gacattccct ttaatggcct cgattttttt
      841 ccacagtgtg ccgggattgg tttcaatttg gtcgtagttc ttgtggaagg cttcgggatc
      901 cagggcttcg cccagcagcg ctttcacttc ggcgttggtc ggccacaagt cgcccagcca
      961 gacatcgccg tttttgcctt tgccaacagg ttcggtcatg aagtcgcgca gtatggtgcc
     1021 ggtcagcgca taggcgacga ttaacggcgg cgaagccagg aagttggcct tgatgttctg
     1081 gtgaattcgg gcttcgaaat tgcgattgcc cgaaaggacg gcggaacaaa ccaaattatt
     1141 cttgttgatg gcttcattga aatccggagt caagtcgccc gagttgccaa tacaggttgt
     1201 gcagccgtag gcagcgaggt tgaatcccag tttttcaagg tacggcagca agcccgtttt
     1261 caccaggtat tcggtgacaa tgcgcgaacc gggcgccaga gatgtcttga tgcgtttggg
     1321 tatcttcagc cctgcttcca ccgctttctt ggccaccagt cctgccgcca gcatgacgct
     1381 ggggttggac gtattggtgc acgaggtaat cgcggcaatc aggatgtcgc cgtttctgac
     1441 ctcggcgcct gcgccggtct tgaaaacctg attcagccgt tctggcggct gactgaagcc
     1501 gttggccgat ttttccgaaa acaatcgggt aaaggtgctt ttgatgtccg ccatctcgat
     1561 ccggtcctgt ggccgtttcg gacctgccag cgaaggggtg acagtggata gatccagggt
     1621 aacgagtttg gtgtaggcga tgtctttggc gtcgggaatg ccgtacatgc cttgggcctt
     1681 gaagtaagcc ttgagcgcat cgacttcgtc tttgctgcgg cctgtgcctt tgaagtattc
     1741 gatcgtccga tcgtcgaccg ggaaaaagcc tatggtcgcg ccatattcgg gcgacatgtt
     1801 gccgatggtt gccctgtcgg tgacggacag gcttgatgta cccgggccgc agaattcgac
     1861 gaagctgccc actacctttt ccttgcgcag catttccgtg attgtcagca ccagatcggt
     1921 cgccgtaacg ccgccgcgca gttcgccttt aagctctact ccgacgacgt ccggagtcag
     1981 gatatacact ggttggccca gcatgcccgc ttcggcctca atgccgccca cgccccagcc
     2041 aatgacgcca atgccattga tcatggtggt gtggctatcg gttccaacca gggtgtccgg
     2101 gtagtacaac ttgttggcgg catcgcaatg tacgccgcgg gcaaggtgtt ccaggttgat
     2161 ctggtgaaca atgccaaagc ccggaggcac gacgccgaag gtatcgaagg cctgtttacc
     2221 ccacttgatg aactggtagc gttcctgatt tcgctggaac tcaagtttca tgttcagacc
     2281 cagcgcgctt ttggtgccgt aatgatcgat cattacggag tgatccacga ccaggtcaac
     2341 cgggaccatg ggctcaatca gttttggatc cctgcttacc ctgtcggcca ccgaacgcat
     2401 ggcggcgata tcgaccagaa gcggcacacc ggtaaagtct tgcaaaatga cgcgcgccac
     2461 tacaaaaggg atctcggcgg tacgctttgc tttgggtttc cagccagcga gctgtttgac
     2521 atgtttttct gttatttttt tgtcatcgca gtttcgcaat actgattcaa gaacaacgcg
     2581 gatgctgacg ggcaggcgtt gtatatcaac gcccagggtt tttccgagcg ccggcaagga
     2641 ataaagctga gccgtttttc gtccaatttt gaatttttgc aagctgttga acttgttgtg
     2701 caacataggg atccttggag tttttaacag ggtgtagcgt gcgtaacgcg tgccgttccg
     2761 gcacgcgcgg aagaattgat ttcgagattg taaaaggaaa gatgcattcg aagcaaatgt
     2821 cttatatctt atataagact taaaaacaac gcacggcctc gcgccccgat catttccgct
     2881 ccgctggcgc tctgaacagg cgccgggcgt attaaacggt cagcgactga tattcaggat
     2941 gcttgcgtat gtaggcaacg gcgtaagagc agacggggtg gaccatccaa ccgttggcct
     3001 gcgcggtatt cagggcatat tgtgtcaact ccgcggcaat gccgcgtccc cccacagccg
     3061 cgggcacgcc ggtataggtg atcgtcattt gcttgccgcg cagcgtgtag tccagtacgc
     3121 acgtgtgccc ctcttcaatg cactcgaacc gatggcgttc cgggttgtga cgaatggcgc
     3181 ggcttgcagg gcgggaggcg ttcatatcgt tcctttggcg tttactattt gttccgaaga
     3241 catattggag gaatccgggc gctgcgccgc cgcccggaag ttctaaagcc tgaaaagcaa
     3301 cagcgtccag acggcaagca tggcaatcag cccaacgaag caaccgcccc acaaaccgac
     3361 cagaggccac tttatggtgg tgggtatttc cttcggatcg atatgtccga gcagacggtt
     3421 ggcctgtcca attatccaga attggctgcc cgggaagcta agccgaaaaa aataatccat
     3481 caggattgcg gcccggatgg cgaccgggta gcgatcgaag ggttgttgtc tcaggtaggg
     3541 gtggcgcagg agttcattgg ttttccgtac cttgaatagt agtagatcca ggcccgatac
     3601 cagcgaggtc agtgcgcata tgaccagtat ggtaaaggcc agcggcagcg cgtaaaggag
     3661 gaatgtgtcg aacataggcg aagcacgtgg ccgacgtagc gagtccatat tgaaaagctc
     3721 ccgcatcgtc gtggtgcgga agcttcggga ttttcctgga tcagatcacg tacagatcca
     3781 cgtattcatg cactggcatt gcctcgagcg cttgctgatt cagtgacaca tcaaggatgc
     3841 gccgctgctg cctggcggga aaccggcgag ccagattgat gcggaacttg ttttcaagca
     3901 gcggaatccc ttcctgacga cggcgtttgt ggccaattgg gtattcgcaa acgatctcgt
     3961 ccaaccggga gccatcgttg aattcgaccg taagcgcatt cgggatcgag cgtttctcgg
     4021 gattatggta gtcggcggtg aaagcgggat cttcccggca aacgatcttg gcgcgcaatg
     4081 cgtcgatacg tgggtcggcg gctattgaat cttcgtagtc gcccgcggtc agacggccga
     4141 aaatgatggg aaccgccacc atatactgta tgcagtggtc ccggtccgcc gggttgttaa
     4201 gcggcccgct tttgtcgatg atccggatgc aggcctcgtg agtgcggatc gttatgcttt
     4261 ttatatcgtc cggcgtcttg ccagccgctt gcagtttctg gtggatatcc atggcgcatt
     4321 ccacagccgt ttgcgaatgg aattcggcgg ggtacgagat tttaaacagc acgttttcca
     4381 tgacgtagct gctgtaaggg cgctggaact tgaagggttg gcctttgaag gagacgtcgt
     4441 aaaagcccca ggttttggcc gtcaggacgg atggatagcc catctcgcct gtcctggcga
     4501 taagtgccag ccgcaccgcc cggctggttg catcgcccgc cgcccagctt ttgcggctgc
     4561 ctgcgttggg agcgtggcga taggtgcgca gactttggcc gtccacccag gccagtgaaa
     4621 tggcattgat ggtttcggcg cgggtcaggc caagcatttg cgagacgacg gctgttgaag
     4681 caactttgac cagaagtacg tgatcaagac ccactttgtt gaacgagttt tcgagggcga
     4741 tgcagccctg aatttcgtgc gccttgatca tgcccgtaag tacgtcgcgc atggttagcg
     4801 gcgcttttcc ggccgccacc gcagtgcgcg agag
//
LOCUS       DATCXU010000302         6109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_87484,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000302 DATCXU010000000
VERSION     DATCXU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87484"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..669
                     /gene="fliE"
                     /locus_tag="VIR04_06695"
     CDS             337..669
                     /gene="fliE"
                     /locus_tag="VIR04_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY9460469.1"
                     /translation="MAIEQLSGIENMLSQMRAAVAAASSSTSGTQDKPAVSGGFAAEL
                     QRSLERVSTMENSATAQAKAFELGAPDVSLNNVMIDMQKVSLALQATVQVRNRLVAAY
                     QEVASMAI"
     gene            complement(678..971)
                     /locus_tag="VIR04_06700"
     CDS             complement(678..971)
                     /locus_tag="VIR04_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY9460470.1"
                     /translation="MAKLKNSPLNDGRPSAVAITYDSHDSAPRVVAKGYGTLAETIIR
                     TAKENGLYVHESPELVGLLMQVDLDKHIPPELYLAIAELLAWLYTLEKRPVTE"
     gene            complement(958..2169)
                     /locus_tag="VIR04_06705"
     CDS             complement(958..2169)
                     /locus_tag="VIR04_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY9460471.1"
                     /translation="MSVGGAPLGTLLIQKLDTALALTSSQQSTIVSGTRPDAVGPLED
                     SNKIDAPQNETLRHPRESVDRTTAQSGRQGQEVVGKTILDARRLNLSMARDVSNISST
                     PSAPTTLGQAAKTILALLVNYPGPAPAVVGETLLPGPTALQLSPDKAAVKATRPDINA
                     GAPAVAGITTGVKGAAPSLAATVAAFVQGLSLAVQGSGLFYESHLNDLAFGKQSIDSL
                     LQEPQGKINQLNTDNGNLTDTRADTARADAPRHASTPANADAAIHTGAATSDGALSGL
                     HPETHLLVRQQLETLANQAFVWRGEAWPDTPIQWELTRREQADTDSGAIADNHWATRI
                     KLTMPNLGEVEAKIKLTGQKMTVHLVAASSAQRLNEHSKALRSRYSALGLQLDQLLIN
                     TDTPVHGNGKT"
     gene            complement(2166..2501)
                     /locus_tag="VIR04_06710"
     CDS             complement(2166..2501)
                     /locus_tag="VIR04_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEY9460472.1"
                     /translation="MSNSNSILRQYQIIASLSGQMLAQARLNQWDSVIILGKQYHEAV
                     EDLRHIDPISGADRLLRGDLLAQILSDDARIRDLATPELARLSLLLGAMRRQQSVFRT
                     YHSSFTSNP"
     gene            complement(2498..3283)
                     /gene="fliD"
                     /locus_tag="VIR04_06715"
     CDS             complement(2498..3283)
                     /gene="fliD"
                     /locus_tag="VIR04_06715"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY9460473.1"
                     /translation="MADSVSSRDVGARLPPGGWLTSARNKESHTLVLILDRQIAVRNR
                     LSAYEKIRSCVARLKSITDTQAKTLSIASDSAAIEAAANELVSAYNDLQTTIHLFTAC
                     DAGANIGAVLTGDSLALRVQNQVRAALNVATSSGAVRSLSQIGIKTQPPCAALAIDAV
                     KLTAALRDDLSSVQELFNGVTGISRQLNIVTSAFLGARGLFGAANDKCNRAIRDLQEQ
                     YDTVWARIEARITTDHTQFTALDTMLMQMNTANRYLTRRLQMP"
     gene            3561..4319
                     /locus_tag="VIR04_06720"
     CDS             3561..4319
                     /locus_tag="VIR04_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEY9460474.1"
                     /translation="MASSEDLLVGQYAPMVRRLALQLVAKLPASVELDDLMQAGMMGL
                     LDAVRRYQRTADAQFETYAVTRIRGAMLDELRSQDWLPRSVRGKSKSIEQAILVLGHR
                     LMRPATELEIAAELNLSLAEYQTLLEEARGVQVVHYEDLARHYVESADPLEHTQTQSG
                     QGELTNPLNQLMSRDLRGALISAIEDLPEREQLLLSLYFEQDLNQKEIGAVMGITEGR
                     VSQLRSQAVARIRASLTEKVWHEKPSEVEYQTLL"
     gene            complement(4333..4830)
                     /locus_tag="VIR04_06725"
     CDS             complement(4333..4830)
                     /locus_tag="VIR04_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460475.1"
                     /translation="MHLFFSVRLKFWLASFMVLWSLGARAAQADWPLAPSHLDLVTPY
                     GDLHVKPSEYIYESRLLINNADADPKIEGILNITYAFATRQAHAALISINNGNSRCPI
                     AYRWVVLDKKGYKMSPEFGSCSPQIKVSSTNRTLVVKTPNNEDPNKIDTYSYDGKTVK
                     RHTQR"
     gene            5206..5610
                     /locus_tag="VIR04_06730"
     CDS             5206..5610
                     /locus_tag="VIR04_06730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY9460476.1"
                     /translation="METIFSRIIKGEIPCAKVFEDDLVFAFMDAGQLNPGHVLVATRT
                     PYETFMDADDASVVALIRAAHRISRAVQRAFKPEGMTLLQANKPAGWQTVPHLHMHVL
                     PRFKNDGVGLIWPRKDPSLDLLLEYARQIKLD"
     gene            5883..>6109
                     /locus_tag="VIR04_06735"
     CDS             5883..>6109
                     /locus_tag="VIR04_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY9460477.1"
                     /translation="MEDNKDGKGALDTGVDRRSVLKMGLGAAGGALLPGVSSVALAAD
                     KPPVGNYPAGVSGDSVFVGLTLDLTGPYSAEG"
BASE COUNT         1345 a         1512 c         1791 g         1461 t
ORIGIN      
        1 gggggagcca ttgtgccggc tggctcattg tttgcctctg tcagtgcaag agcgctgata
       61 cggtaagcac ggcaagcgat ttatgagcaa agtggacatg cggcaagacg aaaacaatgg
      121 gagcttgaat tattgctggg attgcgatgc gaccaagcac cgaaaagcgc cgcttttagg
      181 tgttatttaa gacttatggg cccggaaatg gccaatgtgt gcgggctatc tttgccaagc
      241 gttgaaaaag gcgcgtgttt tgactgttga tgtagcgctt gccatggttg ggttcttgtt
      301 atggtgtggc ctgtggattt ctgcttaggt gagcccatgg caattgaaca attatccggt
      361 atagaaaaca tgctctcgca gatgcgcgca gcggttgcgg cggcatcgtc atccacatct
      421 ggcacgcaag acaagccggc cgtttcgggc ggctttgcgg cagagctgca gcgttctttg
      481 gaacgggtat ccactatgga aaatagcgcc acggcacagg ccaaggcgtt cgagcttggc
      541 gcgcccgatg tttccttgaa caacgtgatg attgatatgc aaaaagttag tctggcgttg
      601 caggcgacgg tgcaggtgag gaatcgcctg gttgccgctt atcaggaagt agccagtatg
      661 gcgatttaat ttgagtttta ttcagtgacg gggcgctttt ccagcgtgta aagccatgcc
      721 agcagctcgg cgatagccag gtaaagctca ggcggaatat gcttgtcaag gtcgacttgc
      781 atcagcaagc ccactaattc gggcgattcg tgaacataca agccgttctc tttagccgtg
      841 cggataatcg tttcagcaag agtcccgtaa cccttcgcaa ccacgcgcgg cgccgagtcg
      901 tggctgtcat aggtgatggc cacagcgctt ggacggccat cgttgagcgg gctattttta
      961 agttttgcca tttccgtgaa ccggagtgtc ggtattgatc aagagctggt caagctgcaa
     1021 acccaaggcg gaataacgtg aacgcaaggc tttgctgtgc tcgttcaggc gttgtgccga
     1081 tgaagcggcg accagatgca cggtcatctt ctgtccggtc agttttattt ttgcttcgac
     1141 ttcgcccaaa ttgggcatgg tgagtttaat tcgtgtcgcc caatggttgt cggcaattgc
     1201 gcccgaatca gtgtccgctt gctcgcgccg ggtaagctcc cattgaatgg gtgtatcggg
     1261 ccaggcctcg ccacgccaca cgaaggcctg attggccaat gtttcgagct gttgtcgcac
     1321 cagcaggtga gtttcaggat gcaggccaga gagcgccccg tcggaagttg cagcaccggt
     1381 gtgaattgcg gcatcggcat tcgccggagt tgatgcatgc cggggtgcat cggcgcgagc
     1441 tgtgtcggcc cgcgtatcgg tcaggttccc gttgtcggtg tttaattgat ttatttttcc
     1501 ttgcggctct tgcaacaaac tgtcgatact ctgtttcccg aacgccagat cgttcagatg
     1561 cgactcgtag aataagccgc tgccttgaac agccagcgaa agcccttgca cgaacgcggc
     1621 cacggttgcc gccaaagacg gcgcggctcc tttgacgccg gttgtgatac ccgccaccgc
     1681 aggcgcgcca gcattgatat ccggccgggt cgcctttaca gcggctttgt ccgggctgag
     1741 ctgtaaagca gttggacccg gcagcagagt ttcaccaacg accgccggcg ctggtcccgg
     1801 atagttgacc aacaaggcca agatggtttt tgccgcctgg cccagtgtgg ttggcgctga
     1861 tggcgtgctg ctgatattgg acacatcgcg cgccatactc agattgaggc gacgggcatc
     1921 cagtatggtt ttgccgacga cttcctgccc ctgccgcccg gattgggcag tagtccggtc
     1981 aacggattcg cgcggatggc gcagtgtctc gttttgcggt gcgtcgatct tgttggaatc
     2041 ttcgagcggg ccaaccgcat cgggccgggt tcctgagaca atagttgatt gctgcgatga
     2101 tgtcagggca agggcagtgt caagcttctg gattaacagg gtccccaacg gagcgccgcc
     2161 gacgctcatg ggttcgaggt aaacgaagaa tgatatgttc gaaagaccga ttgctgccgc
     2221 ctcatggcgc ccagcagcag actcagacga gcaagctcgg gggtcgccaa gtcgcggatg
     2281 cgagcatcat cgctcaggat ttgagctagc agatcgccgc gaaggagacg gtcagcgccg
     2341 cttatcgggt ctatgtgccg cagatcttca accgcttcgt ggtattgctt tcctaagatg
     2401 ataactgaat cccactgatt caatcgggcc tgcgcaagca tctgcccgga caggctggcg
     2461 atgatctggt attggcgcaa aatcgaatta gagttgctca tggcatttgc aaccgccgtg
     2521 tcagatagcg gtttgcggta ttcatttgca tcaacatggt gtccaaggca gtgaattgtg
     2581 tgtggtcggt agttatccgg gcttcaattc gcgcccagac ggtatcgtat tgctcttgca
     2641 agtctctgat ggctcgattg catttatcgt tggcggcgcc aaacaggccc cgcgcgccca
     2701 aaaacgcgct tgtgacaata ttcagttgcc gggagatgcc cgtgacgcca ttgaatagtt
     2761 cttgtacgct ggacaggtcg tctcgtagcg cggcggttag tttaaccgca tcaatggcaa
     2821 gagctgcgca gggcggctgt gtttttatgc ctatttggga cagcgaacgg acggcgcctg
     2881 aagacgtcgc cacattcaaa gcggcgcgga cctgattctg cactcgaaga gccagagagt
     2941 cgccggtcag gaccgcgccg atgttggcgc cggcatcgca ggccgtgaac aagtgaatcg
     3001 tcgtttgcaa atcgttgtag gccgatacga gttcgttcgc ggccgcctct atggcggcgc
     3061 tgtcggaagc gattgataaa gtttttgcct gagtatcagt aatgctttta agccgtgcca
     3121 cgcagcttcg tattttttcg taggccgata agcggtttcg aacagcgatc tgccgatcca
     3181 gaataagaac aagggtatga ctttctttgt tgcgcgcaga tgtaagccag ccgcctggcg
     3241 gcaagcgggc gccgacgtcg cgtgaagaaa ccgaatcagc cataaaccat ctccctgaaa
     3301 ggtttattgt caggcgaatt gacaagattt aatgcgcttg ataacagcca tatcggttgt
     3361 tacattggcc gtttaggacg agcatcgagt caggatgagc ccgatgccat acgcctttcg
     3421 ttttaacggt cgttgcctga caaactttag gctctgcttc aaatcatgga atagccaata
     3481 gaatcgtgca ctgatcggtc ggtgaacagt tcagaaagcg ggcgacaata gactttctgt
     3541 atttggtgcc tctcattgac atggctagtt cggaagacct cctggtcggg cagtacgctc
     3601 ctatggtgcg acgccttgct ctacaactgg tggcaaagtt gcccgccagt gtagagctcg
     3661 acgacttgat gcaggccgga atgatgggct tgctcgacgc ggtgcgtcgt tatcagcgca
     3721 cggccgacgc ccagttcgaa acctatgccg ttacgcgcat acgcggcgcg atgctcgatg
     3781 aacttcgcag ccaggattgg cttccgcgca gtgtgcgcgg caagtccaaa agcatcgaac
     3841 aggcgattct tgttttggga catcgcctta tgcggccggc gaccgagctt gaaatcgccg
     3901 cggagctcaa cttgtctttg gcggaatatc agacgcttct cgaagaggca cgaggcgtgc
     3961 aggtcgttca ctacgaggat ttggcgcggc attatgtgga atcggccgat ccgctcgaac
     4021 acacccagac gcagtccggg cagggcgagc tgaccaaccc gctaaaccaa ttaatgtcgc
     4081 gcgatttgcg tggtgcgctg atttcggcca ttgaagatct gcctgaacgc gagcagctat
     4141 tgctgtcttt atactttgag caggatttga accaaaagga aatcggcgct gttatgggta
     4201 taaccgaggg ccgggtgtcg caactgcgct ctcaggcggt tgcgcgaatt cgcgcttcgc
     4261 tgaccgagaa agtatggcac gaaaaaccca gtgaagtcga atatcagacc ttgctgtaag
     4321 cgccgtcaaa aactaacgct gagtgtggcg cttgacggtc tttccgtcat aactgtatgt
     4381 atctatttta ttcggatctt cgttattcgg ggttttcaca accagagtgc gattcgtgga
     4441 tgagaccttg atttgcggcg agcatgaccc gaactcaggc gacattttat agcccttttt
     4501 atccaggact acccagcggt aggcgatggg acagcggctg ttgccattgt tgatggaaat
     4561 gagggccgca tgcgcctgac gcgtcgcgaa ggcgtaggtg atgttaagga ttccctctat
     4621 tttgggatcg gcgtcggcgt tattgatgag cagcctggat tcgtagatat actcgctggg
     4681 tttgacgtgc aaatcgccgt aaggcgtgac cagatcaaga tgggagggag ccagcggcca
     4741 atcggcctgc gccgctcgcg cgcccaatga ccaaagcacc atgaagctgg ccagccagaa
     4801 cttgagtcgc acggaaaaga agagatgcat gatgcctgtt gaatatgatg ttacaaaaca
     4861 aagacgaaag cggctctttt tgtaaagatc attacatttc attttggaaa taagtgcaac
     4921 tcagtcgttc agcttagatt tcatgcgcga tcatgatttg atcataagat acagaagcgc
     4981 aacgaacaca aggccgaaat agacagcgac ccccattaat aagctgtgca agacgatttt
     5041 cacgtgttgt gaaaatgtca gcttgccggt tgttcggctc ttgtaagtag cccaccacca
     5101 cgtgccggcg gcggcgcaaa ggccgagtaa cagtattgct atcttcattg tcgtatagta
     5161 ggcggcaaaa cgcgtgttag aaagaaaatc agaaaggagg ccgcgatgga aaccattttt
     5221 tcaagaatca tcaaaggtga aattccttgc gcaaaagtat tcgaggacga tttggttttc
     5281 gcttttatgg acgctggaca gcttaacccc ggccatgtgc tggtggcgac caggacgccc
     5341 tacgaaacgt ttatggatgc ggacgacgca tcggtggtgg ctttgattcg ggccgcgcat
     5401 agaatttccc gtgcggtgca gcgcgcattc aagccggagg gcatgacgct attgcaggcc
     5461 aacaagccgg cgggctggca gacggtcccg catttgcata tgcacgtatt gccccgtttt
     5521 aaaaacgatg gcgtcggttt gatctggccg cgcaaggatc cgtcgctgga tcttttgctc
     5581 gaatatgccc gccagattaa attggattga aggaaaatca cgatactgaa ggggtttcaa
     5641 tgaaggtttt ccctatggcg aaatctcttt gcatttttat cgcgccgcga tatattaaaa
     5701 cgaaattgtc gtaactagat ataaatatat cgcagcatcg gggacacgca tcccattttt
     5761 tattgcgcac tgagcgacgc gggccgttct gtgcgcaata ttgttgcaac ataccaaacc
     5821 gacgcccaag attggtcttg tttcaggcaa tcattttgtt cgcaaacact aggagagtaa
     5881 ggatggaaga taataaagac ggcaaaggcg ccttggatac cggcgtcgac cgtcggtcgg
     5941 tgcttaagat gggtctggga gcggccgggg gcgctttgtt gccgggtgtt tcgagtgtgg
     6001 cattagcggc cgataaaccg cctgttggca attacccggc gggcgtaagc ggcgattcag
     6061 tcttcgttgg cctgactctc gatttgaccg gaccttattc ggccgaagg
//
LOCUS       DATCXU010000303         6357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1154122,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000303 DATCXU010000000
VERSION     DATCXU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154122"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIR04_06740"
     CDS             complement(<1..305)
                     /locus_tag="VIR04_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY9460478.1"
                     /translation="MRGIVIGMLLTVSLVRCVYAAPPEVVIDPGGVPPVALQSIVGAV
                     NAITRLAEDQDVGEITRLRRRARDATLSALQTQGYFSAKVTLEAGHDAGGETWNIAI"
     gene            complement(311..1393)
                     /gene="apbC"
                     /locus_tag="VIR04_06745"
     CDS             complement(311..1393)
                     /gene="apbC"
                     /locus_tag="VIR04_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY9460479.1"
                     /translation="MSSIEQVRALLAGVTDPNTKKTIGNAAQDSELQLDGTQLRLTVA
                     LGYPIHNGQPELQKRIETALQPLGLEIKELRFKTHIGSHAVQRGQRTLRNIRNIIAVA
                     SGKGGVGKSTTAVNVALALSVQGARVGLLDADIYGPSVPIMLGLSGRPKVLDGKVMQP
                     MVGHGLQSISIGFLIDEDSPAIWRGPMVTQALVQLLNQTAWDDLDYLIVDMPPGTGDI
                     ALTMAQKVPLTGAIIVTTPQDLALADARKGLLMFQKVDVPVLGVVENMSTHICSNCGH
                     AEPIFGENGGRNMAAEFNLPWLGSLPLVMNIRTQTDSGNPTVIAEPDGEIAAIYHDIA
                     RKIAAIVAVLPLDTAGVRPKVVARTS"
     gene            1485..1733
                     /locus_tag="VIR04_06750"
     CDS             1485..1733
                     /locus_tag="VIR04_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460480.1"
                     /translation="MNTFNQLLRGALFLVLALFGVTMAFIFMVSTAIAVAVLYVVARI
                     KGRPFGVKAYWDARNRTESRKNNFKNKDVVDIEMRELP"
     gene            1873..3942
                     /gene="metG"
                     /locus_tag="VIR04_06755"
     CDS             1873..3942
                     /gene="metG"
                     /locus_tag="VIR04_06755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY9460481.1"
                     /translation="MPRTLFVTTALPYANGSFHIGHIMEYIQADIWVRSMRMSGHTVH
                     FVGADDAHGAPIMLKAESEGITPQALVARYANERAQYLDGFHIKFDHWHSTDSPENVA
                     LSQDIYRRLKAAGFIDSRTIEQFYDPVKGMFLPDRYIKGECPRCHAKDQFGDSCESCG
                     AVYAPTDLINPYSALTHATPILKASDHFFFKLSDPRCVEFLRSWTKGKNAHGNPHLQA
                     EMLAKTREWLGSNQDGDSNLSDWDISRDSPYFGIEIPDAPGKFFYVWLDAPVGYLASL
                     KAYCKKQNIDFDALLDPNGDTEQVHFIGKDIVYFHALFWPAMLKFSGRKTPDGLHVHG
                     FITVSGEKMSKSRGTGISPLRYLELGMNAEWLRYYIAAKLNARVEDLDFNPDDFIARV
                     NSDLIGKYINIASRAANFINKHFDGRLDYLGDVQALKEELSQTAEAARADFEAREYGR
                     AIRGAMAYADRINQDFDMAAPWVTAKGITTASAEQKALLQNVCSRALAGFKTLSIILS
                     PVLPALTERVAQQLFGAAQNYRWSDAGALPTHIAAFKHLMERVDPKMLDDLFEAPAEA
                     AILPGGEAVADLIDIKDFAKIDLRIAKIIDCTTVDGSDKLLRLTLDAGEGRNRQVFSG
                     IKSAYTPADLIGKLTVLVANLTPRKMRFGVSEGMVLAASHNNPDVDAGIYILEPWVGA
                     KPGMRIG"
     gene            3982..4935
                     /locus_tag="VIR04_06760"
     CDS             3982..4935
                     /locus_tag="VIR04_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY9460482.1"
                     /translation="MDKHFDTQRRFDPKRQKAGRRSTLVSILVNTLLTTIQFAIGMLA
                     HSQALVADAIHSLSDLISDFVVLAANKHSHKAPDADHQYGHLRFETAASLMLGALLLA
                     VGFGMLWSAFVKLQSPANVPTVHAIALIVALMALIVKELLFRYMLTVAKRQRSSMLVA
                     NAWHARSDAASSLVVAIGIVANLSGFPLADPLAALIVGLMVGRMGWKFFFESFNDLMD
                     RAVDAQTESRIRTHLLSTPGVQGIHDLKTRKLGDMIWVEVDLEMNATLTIEQGHAIAV
                     AARDRVMQREPVLDVMTHFDPVSVVRVGASATPGHEKNWSG"
     gene            complement(4924..4997)
                     /locus_tag="VIR04_06765"
     tRNA            complement(4924..4997)
                     /locus_tag="VIR04_06765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4963..4965),aa:Gly,seq:ccc)
     gene            5189..5536
                     /locus_tag="VIR04_06770"
     CDS             5189..5536
                     /locus_tag="VIR04_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9460483.1"
                     /translation="MDQPVFPTATKELAKQRAELAPDTLAAFKAFSASVFAEGALPKK
                     TKQLIAVAVAHVTQCPYCIRGHTEAALRSGATDKEIMEAIWVAAEMRAGGAYAHSNLA
                     LDTINHVHTQHGG"
     gene            5540..6265
                     /gene="trmB"
                     /locus_tag="VIR04_06775"
     CDS             5540..6265
                     /gene="trmB"
                     /locus_tag="VIR04_06775"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY9460484.1"
                     /translation="MAGADTPAKLDSTLQDPPGARPPIRSFVHRRSHITPAQQQALER
                     LAPQWTVPYRAQVLNIEQAFGRAAPTVLEIGFGMGETTEKIAASRAENNFLGVEVFNA
                     GVGALLKRIELSGLQNIRIIQHDAVEVLRDMIAPNCLAGAHIYFPDPWPKTRHHKRRL
                     IQPPFVDLLTSRIMPGGYIHCATDWENYAEQMLAVLSAELRLSNTSEGYAARPDFRPQ
                     TKFETRGLRLGHGVRDLIFIRKE"
BASE COUNT         1467 a         1894 c         1669 g         1327 t
ORIGIN      
        1 tcaatcgcaa tattccatgt ctcgccgcca gcatcatggc ctgcctcgag cgtgactttc
       61 gccgaaaaat aaccctgcgt ttgcagcgcc gacaacgtcg catcgcgcgc acggcgacgc
      121 aaacgcgtga tttcgccgac atcctgatct tcggccagac gcgtgatggc attcaccgcg
      181 ccgacaatcg actgcagggc gacgggcgga actccgcccg ggtctatgac gacctctggc
      241 ggcgccgcgt aaacgcaccg gaccaggcta acagtcagca acatcccgat aacgatgcca
      301 cgcatcagaa tcaggatgtg cgcgccacga cttttggccg caccccagcc gtatccagcg
      361 gcaatactgc aacgatagcg gcgattttac gagcaatgtc atggtatatg gccgcaattt
      421 caccgtcggg ctcggcaatg acagtcgggt tgcctgaatc cgtttgagtg cgaatattca
      481 tcaccagcgg caaagatcca agccagggca agttaaactc ggccgccatg tttctgcccc
      541 cgttttcccc gaaaataggt tcggcgtggc cgcagttcga gcaaatgtgc gtagacatgt
      601 tttcgaccac gcccagcacc ggcacatcga ccttctgaaa cataagcaga cctttacgcg
      661 cgtcggccaa agccaagtcc tgaggcgtcg tcacaataat cgcccctgta agcggcacct
      721 tttgcgccat ggtcagcgcg atatcgccgg tgccgggagg catatccacg atcagataat
      781 caagatcatc ccacgccgtc tggtttaata actgcaccag cgcctgcgtc accatgggtc
      841 cgcgccagat agccggcgaa tcctcatcga taagaaatcc gatcgaaatg gactgcaaac
      901 catgtccgac catgggctgc atgacctttc cgtccagaac cttggggcgg cccgacaggc
      961 ccagcataat gggtacgctg gggccataaa tatccgcatc cagcaagcct actctggcgc
     1021 cctgaaccga cagggccagc gccacattca ccgccgtggt gctcttgccc acgccccctt
     1081 tgccggaagc cactgcgata atgttgcgga tatttcttaa cgtccgctga ccacgctgta
     1141 cggcatggct gccaatatgt gttttaaaac gcaattcctt gatttccagc cctaaaggct
     1201 gcaaagcggt ttcaatacgt ttctgcagtt cgggttgccc attgtgtatc gggtagccaa
     1261 gcgctacagt aagacgtaat tgcgttccgt ccaactgcaa ttcgctgtct tgggcggcat
     1321 ttcctatggt ctttttagta ttaggatcgg tcactccggc caacaaagcg cgtacttgtt
     1381 caattgagct cataaaacag gcctcttaaa gaattcacca ttcgaaccct tagaatagcg
     1441 gattgccgga attattttct gacacggagg tctatccatt caagatgaat acatttaatc
     1501 aattacttcg cggcgcgctg tttcttgtgc tggccttgtt tggcgtaacc atggccttta
     1561 ttttcatggt ttcaacggcc attgctgtgg ctgtgctgta cgtcgttgcc cgcatcaaag
     1621 gccgtccttt tggcgtgaag gcctactggg acgcgcgcaa ccgaaccgaa tcacgaaaaa
     1681 ataacttcaa gaacaaagac gtggtcgaca tcgaaatgcg cgaactgcct taaccggcgc
     1741 gcggcggcac cctggttgcg ttcattacac tgccgcggtg gcgtaacaaa ccgtaaattt
     1801 tcgcaagcgc gtaaaacctg acattcggca acagtcagta caatgcaccc ttttacgcac
     1861 actactacaa acatgccgcg cacgctattt gtcaccaccg ccttgcctta cgccaatgga
     1921 tcattccata ttggccacat catggaatat attcaggccg acatctgggt tcggtccatg
     1981 cgcatgtcgg gccatactgt gcatttcgtg ggcgctgacg acgcccacgg cgcgcccatc
     2041 atgctcaagg cggaaagcga aggaattaca ccgcaagcgc tcgtggcgcg ctacgcgaac
     2101 gaaagggctc aataccttga cggcttccac atcaagttcg accactggca ttccaccgat
     2161 tcgcccgaaa acgttgcgct gtcgcaggac atttatcggc gtcttaaggc ggccggattc
     2221 atcgactcgc gcaccatcga gcagttttac gatccggtca aaggcatgtt tttgcctgat
     2281 cgttatatca aaggagaatg cccgcgctgc cacgccaaag accaatttgg cgattcctgc
     2341 gaatcctgcg gcgcagtcta tgccccgacc gacttgatca acccctactc ggcgctgact
     2401 cacgcaacgc ccatactgaa agcatccgac cattttttct tcaaactatc ggatccacgt
     2461 tgcgtcgaat tcctgcgctc ctggacaaaa ggaaaaaatg cccatggcaa cccgcatctg
     2521 caggcggaaa tgcttgccaa aacacgcgaa tggctgggct cgaaccaaga tggcgattcg
     2581 aatctgtcgg actgggacat ttcgcgcgat tccccatact tcggcattga aattccggac
     2641 gcgccgggca aattctttta tgtgtggctt gacgcgcccg tgggctacct ggcgtctctg
     2701 aaagcctatt gcaaaaaaca aaacattgac ttcgacgccc tgctggaccc caatggcgac
     2761 accgagcaag tgcatttcat cggcaaagac atcgtctatt tccacgcgct gttttggccg
     2821 gccatgctta aattttcggg ccggaaaacg cctgatggtc tgcatgtgca tggatttata
     2881 acagtcagcg gcgaaaaaat gtccaagagc cgcggcacgg ggatatctcc gctgcggtat
     2941 ctggaactgg gcatgaacgc ggaatggttg cgttattaca ttgcggccaa actgaacgcg
     3001 cgcgtcgaag acctggactt caaccccgac gatttcattg cccgggtcaa tagtgacttg
     3061 atcggcaagt acatcaacat tgcaagccgg gcggccaact tcatcaataa gcacttcgac
     3121 ggccggttgg actatctggg cgatgtgcag gcattaaagg aagagctttc ccaaacggcg
     3181 gaagcggcgc gcgccgattt cgaggcgcgt gaatacggcc gggccatccg cggcgccatg
     3241 gcgtacgccg accgcataaa ccaggatttc gacatggcgg caccctgggt cacggcgaag
     3301 gggatcacca cagccagcgc tgaacaaaaa gccttgctgc aaaatgtatg ctcacgcgcg
     3361 ctggccgggt tcaaaacgct ttcgatcatc ctcagccccg tgctgccggc gctgaccgag
     3421 cgagttgcgc aacagttgtt tggcgcagcg caaaattacc gatggtccga cgccggcgcg
     3481 ttacccacgc acattgccgc attcaaacat ctgatggagc gcgtcgatcc gaaaatgctt
     3541 gatgatttat tcgaagcgcc cgctgaagcc gccatactgc ccggaggcga ggcagtcgcc
     3601 gacctaatcg acattaaaga tttcgccaaa attgatttgc gcattgccaa aatcatcgat
     3661 tgcacaacgg tcgacggatc ggacaagctt ttgcgcctta ctctggacgc gggggaagga
     3721 cgaaaccgcc aggttttctc ggggataaaa tccgcctaca cgccggccga tctgatcggc
     3781 aaactgactg ttcttgtcgc caacctgaca cccaggaaaa tgcgcttcgg tgtgtcagaa
     3841 ggcatggttc tggccgccag ccacaataac ccggacgtcg acgcgggcat ctacatactg
     3901 gagccttggg tgggagcgaa accgggaatg cgcatcggtt gaccgcacgg tcaatccgcc
     3961 gatcggccat aatgcagttc tatggataaa catttcgaca cgcaacgccg gttcgaccca
     4021 aaacgccaaa aagccggacg ccgcagcacg ctggtcagca tcttggtcaa cactctgctg
     4081 acaacaatcc aatttgccat aggcatgctt gcgcattccc aagccctggt ggcggacgct
     4141 attcattcgc tgtcggacct gatatccgat tttgtcgttt tagccgccaa caaacatagc
     4201 cacaaagcgc ccgatgcgga ccatcaatac gggcatttgc gcttcgaaac cgctgcatcg
     4261 cttatgttgg gggcattgct gctcgccgtc ggtttcggca tgttgtggtc cgcctttgta
     4321 aaactgcaaa gccccgccaa cgtccccacc gtgcatgcca ttgcgctgat cgtggcatta
     4381 atggctctga tcgtcaaaga actgctgttt cgctatatgc tcacggtggc caagcggcag
     4441 cgctcgtcca tgctggtcgc aaacgcctgg catgcgcgtt ccgacgccgc ctcgtccttg
     4501 gtcgttgcca ttggcatcgt ggccaatcta agcggcttcc cattggccga ccccttggcg
     4561 gcgttaatcg tgggccttat ggtggggcgt atgggctgga agtttttttt cgaatcgttc
     4621 aacgatttga tggaccgcgc tgtcgacgcg caaactgaaa gccgcattcg cacccatctg
     4681 ctctcgacgc cgggagtaca aggcatacac gaccttaaaa cgcgcaagct gggcgatatg
     4741 atctgggtcg aggtcgatct ggaaatgaac gcgaccctga caatagaaca gggccacgcc
     4801 attgcggttg ccgcccgtga ccgcgtcatg cagcgcgaac ccgtgcttga tgtcatgacg
     4861 cactttgatc cggtcagcgt ggtgcgcgtg ggtgcatcgg caacgccagg tcacgaaaaa
     4921 aactggagcg ggtgaaggga atcgaaccct cgtcgtaagc ttgggaagct tctgctctgc
     4981 cattgagcta cacccgcgcg gtgacgcatt ttacgcactt tcgattcgat tccgcaaacg
     5041 cggcaaagca cattcaatcg gcttcaagcg ccgcgaaaca tcgcatgcca taagcgctat
     5101 gatagcaatc aacacgcaga gacaagtcgt cgcgctctga cgcaacactc gaagaactct
     5161 gcgtgacccc aatccaggag aataccgcat ggatcaacct gtatttccca ccgcgacgaa
     5221 agaacttgcc aaacaacgcg ctgaactggc gcccgacacg ctggccgcat tcaaggcttt
     5281 tagcgcaagc gttttcgccg aaggcgcact gccaaagaag acaaaacaac ttattgccgt
     5341 ggcggtcgcg cacgtcaccc aatgccctta ttgcattcgt ggtcataccg aggccgcgct
     5401 gagaagcggc gcaaccgaca aagaaataat ggaagcaata tgggtcgcgg cggagatgcg
     5461 cgccggcggc gcttatgcgc attcaaatct tgctcttgat acgatcaatc acgtgcacac
     5521 tcaacacggc ggctgattta tggccggcgc cgacacaccc gcaaagctgg acagtacgct
     5581 gcaggatccg ccgggcgcgc gccctcctat ccggagcttc gtgcatcggc gcagccacat
     5641 caccccggcc cagcaacaag cactcgagcg ccttgcgccc cagtggaccg tgccctatcg
     5701 ggcacaggtt ctgaatatcg aacaagcgtt tggccgcgcc gcgccgactg tgcttgaaat
     5761 cggcttcggc atgggcgaaa ctaccgagaa aatagcggcg tcgcgcgctg aaaataattt
     5821 tttgggcgtg gaagtcttta atgccggagt gggcgcttta ctcaagcgca tagagctatc
     5881 ggggctgcaa aatatccgca taatccagca cgacgcggtc gaagttctgc gagacatgat
     5941 cgcgcccaat tgcctggccg gcgcacatat ttattttccc gacccttggc cgaaaacgcg
     6001 ccatcacaaa cgccggctga tccagccgcc tttcgtcgat ctgctcacca gccgcattat
     6061 gccgggcggc tacatacatt gcgcgaccga ctgggaaaac tacgccgaac aaatgctggc
     6121 tgtgctgagc gccgagctgc gcttgtccaa caccagcgaa gggtacgccg cgcgacccga
     6181 ttttcgcccg caaaccaagt tcgagacgcg cggcctgcgc ctgggacatg gtgtgcgcga
     6241 cctaattttc attcgcaagg aataaccatg taccccgaga tacaacccta tcgtcagggc
     6301 taccttaata ccgacgacgg acaccaggtt tattgggagc tatgcggcaa cccggcc
//
LOCUS       DATCXU010000304         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_3562, whole
            genome shotgun sequence.
ACCESSION   DATCXU010000304 DATCXU010000000
VERSION     DATCXU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VIR04_06780"
     CDS             complement(<1..255)
                     /locus_tag="VIR04_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY9460485.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQQSGPVTLATISQRENISLSYLEQ
                     LFGKLRRHKLVDSIRGPGGGYTLARLARNITIADIIFAVDE"
     gene            complement(419..910)
                     /locus_tag="VIR04_06785"
     CDS             complement(419..910)
                     /locus_tag="VIR04_06785"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY9460486.1"
                     /translation="MMTKVLFVCMGNICRSPSAEGVFRHLIDDSGLSDVVGVDSAGTH
                     SFHIGEPPDARAQAAARKRGYDISGCVARQVVADDFREFDYILAMDWENLAALQQRCP
                     KIYQHKLMLLMRFANEFEEAVVPDPYYGGPEGFNKVLDHLEDACQGVLELVRKRATQY
                     QAA"
     gene            complement(1045..>2912)
                     /gene="uvrB"
                     /locus_tag="VIR04_06790"
     CDS             complement(1045..>2912)
                     /gene="uvrB"
                     /locus_tag="VIR04_06790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY9460487.1"
                     /translation="SGKTYTMANVIARTGRPALVLAPNKTLAAQLYSEMREFFPNNAV
                     EYFVSYYDYYQPEAYVAARDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVGTVSC
                     IYGIGNPGDYHAMVLTLRAGDRIPRQEILARLVTMQYERNDVEFVRGTFRARGETIDV
                     FPAEHAELALRITLFDDEIESLEMFDPLTGKIRQKTPRFTVFPSSHYVTPRDTVLRAI
                     GTIKDELRDRLAFLVGEGKLVEAQRLEQRTRFDLEMLQELGFCKGIENYSRHLSGAEP
                     GEPPPTLIDYLPADALMFVDESHVTIGQLGGMYRGDRSRKETLVQFGFRLPSALDNRP
                     LRLEEFEHRMRQCVFVSATPAVYEKEHADNIVEQVVRPTGLVDPAIEVRPATTQVDDL
                     LGEVKKRTAIKERVLVTTLTKRMAEDLTDYLIESGIKARYLHSDIDTVERVEILRDLR
                     LGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNIHGRAI
                     LYADHITESMRRAIDETGRRRTKQEAFNKAHGITARGVNKAVRDLIDGVMSVAAQAAA
                     LDDIPPDVLADDKSLSREIRRLEKLMMDHARNLEFEQAAAARDALNRLKQRALLS"
BASE COUNT          663 a          857 c          746 g          646 t
ORIGIN      
        1 ctcgtcaacg gcaaaaataa tgtccgcgat cgtgatattg cgcgccaagc gcgccagcgt
       61 gtagccgccg ccgggaccgc gtatgctgtc gaccagctta tggcggcgca atttgccaaa
      121 caattgttcc agataagata gcgaaatgtt ttcgcgctgg ctgatggtag ccaaagtgac
      181 cgggccgctt tgctgccgta atgccagatc gatcatagct gtcaccgcaa aacgcccttt
      241 ggtcgtcaaa cgcatggtga ttcccttgcc aaagcctaat tgcgcagtga attagccggc
      301 ggctttatac ctgactaatt agctcaagta taaccaattc ccgactgatt ttgtggggtt
      361 tagataacaa aaaaaccggg cgagccccgg cttttttgtg actacgatgc gtttggcgtc
      421 aggccgcctg atactgggtg gcccgtttgc gtacaagctc aagcaccccc tgacaggcat
      481 cttcaaggtg gtccagcacc ttgttgaacc cttcggggcc gccatagtac ggatccggga
      541 cgaccgcttc ttcgaattca ttggcaaagc gcattaacaa catgagcttg tgctgataga
      601 tcttggggca acgctgctgc agcgccgcaa gattttccca gtccattgcc aatatgtaat
      661 cgaactcgcg aaagtcgtcg gctacaacct gccgcgccac gcagcccgaa atatcgtaac
      721 cgcgctttcg cgctgccgcc tgcgcgcgag catcaggagg ttcgccaatg tgaaagctgt
      781 gggttccggc cgagtcgact ccgacaacat cggataaacc cgaatcgtcg atcaagtggc
      841 ggaatacgcc ctctgcgctg ggtgagcgac agatattacc catgcaaaca aaaagtacct
      901 tagtcatcat ggtgtaaagt atgagggata acccgcaaca aggcaagact ttttgcagta
      961 gaaaccgcta tatcgtcaat tttcgtgcgc cgcacaaatg cgcccgtgaa ggcagtgttg
     1021 gtcttttcca acacaagcga cgcgtcaact taataacgca cgctgcttca gacgattcag
     1081 cgcatcgcgc gcggcggcgg cttgttcgaa ctccagatta cgggcgtgat ccatcatcag
     1141 tttctccagg cgccgaatct ccctcgacaa ggacttgtcg tctgccagga catcgggcgg
     1201 tatgtcatct agcgccgccg cttgagcggc gacggacatg accccgtcaa tcaggtcgcg
     1261 cacggctttg ttgacaccgc gagcggtaat accatgtgct ttgttaaatg cctcttgttt
     1321 tgtgcgtcgg cggccggttt cgtcaattgc acggcgcatg gactccgtaa tgtggtccgc
     1381 atacaaaata gcccgcccgt ggatattgcg cgccgccctc cctatcgttt gaatcaaact
     1441 gcgctccgaa cgcagaaaac cttctttgtc ggcatcaaga atcgccacca gcgacacctc
     1501 ggggatatcc agaccctcgc gcaataaatt aataccaacc agtacatcaa atgtgccgag
     1561 gcgcaaatcg cgcaaaatct cgacccgctc cacggtatca atatcggaat gcaaataacg
     1621 cgccttgatc ccgctttcaa tcaaatagtc agtcaaatcc tcggccatgc gcttggtaag
     1681 tgtcgtgacc agcacgcgct ctttaatggc tgtacgtttt ttaacttcac ccagcaaatc
     1741 atccacttgg gtcgtcgcgg gacgcacttc aatagccgga tcaaccaacc ctgtcggccg
     1801 caccacctgc tcgactatat tgtcggcgtg ttccttttcg tacacggccg gcgtagccga
     1861 cacgaaaacg cattgacgca tgcgatgttc gaattcctcg agccttagcg gccgattgtc
     1921 cagcgccgac ggaaggcgaa acccgaactg caccaacgtt tctttacggg aacgatcgcc
     1981 gcggtacatg ccgcccaatt gccctattgt gacgtggctt tcatcaacaa acatcagggc
     2041 gtcggcgggc aagtaatcaa tcagggtcgg tggcggttcg cccggctccg ccccggacaa
     2101 atggcgcgaa taattttcaa tacctttgca aaaccccaac tcctgcaaca tttcaaggtc
     2161 aaagcgggta cgctgttcaa gccgctgcgc ctcgaccagc ttgccttcac cgaccaaaaa
     2221 cgccagccgg tcgcgcaact cgtctttaat agttccaatc gcgcgcaaca ccgtatcgcg
     2281 cggcgtcacg tagtgcgagc tgggaaacac cgtaaagcgc ggcgtctttt gcctgatctt
     2341 gccggtcagc ggatcgaaca tttccagcga ctcgatttcg tcatcgaaca gtgtgatgcg
     2401 cagtgcaagt tcggcgtgct cggcgggaaa cacatcaatg gtttcgcccc gcgcccgaaa
     2461 cgtgccacgc acaaattcca cgtcattgcg ctcgtactgc attgtcacca ggcgcgccag
     2521 gatttcctgt cgcgggatgc gatcccccgc ccttaacgtc agcaccatcg cgtgataatc
     2581 gcccggattg ccgatgccat aaatgcacga tacggtgccg acaataatcg tatcgcgccg
     2641 ttcaagcagg cttttggttg ccgacaaccg catctgctcg atatgctcgt tgatggatga
     2701 gtctttttcg atgaacaggt cgcgcgcggc gacataggcc tcgggctgat agtaatcgta
     2761 atatgacaca aagtactcga ccgcattgtt cggaaagaac tcgcgcattt cggaatacag
     2821 ttgcgccgcc agtgttttat tgggagccag caccagcgcc ggccgccctg tacgggcaat
     2881 cacgttggcc atggtgtagg ttttccccga gc
//
LOCUS       DATCXU010000305         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_965750,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000305 DATCXU010000000
VERSION     DATCXU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965750"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VIR04_06795"
     CDS             complement(<1..952)
                     /locus_tag="VIR04_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY9460488.1"
                     /translation="MENIDALFHGFSILMTWYNLGMMMIGLVLGVIVGVLPGLGGPNG
                     VAILLPLTFGMSPTSAIIMLSCIYWGSLFAGAITSILFNIPGEPSSVATTFDGYPLAQ
                     QGRAGEALTASFSGSFVGAFVGVLLITFLAPMVSKFALRFGPAEFFAVFLLTFCSFVG
                     TDRKAPFKTIISMMLGFGLAEIGMDTISGQLRMTFGLPGLLRGIDFLVVVIGLFGIGE
                     ILVSMEEGLAFEGKSGKINLKVVWQTWKKLPKYWLTLLRSTAIGCWMGVTPGGATAAS
                     FMGYGIAQRFSPRADKFGTGELEGVVAPETAAHAAGTSALL"
     gene            complement(977..1492)
                     /locus_tag="VIR04_06800"
     CDS             complement(977..1492)
                     /locus_tag="VIR04_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY9460489.1"
                     /translation="MEQHETPENPSGGGFTYSVVDALTAVVIFIVGAVMMADNYRIGS
                     GWGSEGPQPGYFPFHIGAILCISAVAVLLKSLFGKHRNHVVFVTWSRFRLVLIVLIPT
                     ALYVLVVQLIGIYVASTIFIGGFMRLLGKTSWLKTIAVSVGVSVVLFWMFEIQFMVPL
                     PKGPLEALFGY"
     gene            complement(1515..2480)
                     /locus_tag="VIR04_06805"
     CDS             complement(1515..2480)
                     /locus_tag="VIR04_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460490.1"
                     /translation="MMACLAAPAQAEKWEPTRTVQIIVPAGAGGASDQMARTIQNIII
                     THKLMKPSSLVLNEGGASGAEGIMDAKGSKGDPYKLMVAFSAIYTLPIAVNLPFNWRD
                     LNPVAMIAQDEFLLWVNADTPYKTAADYLNAVKAAPRGKFKMGGTGAKREDQIITVAL
                     DKAAGVHFTYVPYKSGGEAATQLVGKHTDSNVNNPSENIAQWRAGQLRALCVFSEQRM
                     AYTEKVTKDQSWGDIPTCKESGYDVQYQMLRAFFLPPGTTQEQVDFYADMLKKVSETP
                     EWKAYIAKQALHADFRTGKAFTDFLTKDEAYHKTVMQEAGLAAKK"
     gene            complement(2622..3809)
                     /locus_tag="VIR04_06810"
     CDS             complement(2622..3809)
                     /locus_tag="VIR04_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY9460491.1"
                     /translation="MITFNSHPSGRHFLQIPGPTNVPDRVLRAIDNATIDHRGPEFGE
                     IGKTVLAGMKKVFKTKGDVVIYPASGTGAWEAALVNTLSPGDLVLMAETGHFAALWKK
                     MAVKLGLKVEFLPGDWRRGADAATIEARLTSDKEHAIKAVCIVHNETATGVTSNIPAV
                     RHAIDRANHPALFMVDTISSLASVDYRHDEWGVDVTVSGSQKGLMLPPGLSFNAISAK
                     ALAASQTSKLPRSYWDWHPMLDINPTGYFPYTPATNLLYGLHEALEQLFEEGLDNVFA
                     RHIRHGEATRRAVRTWGLEILCANPAEYSPALTAVIMPKGHSADAFRKVVLEHFNMSL
                     GQGLSKLKDTVFRIGHLGDLNDLSLCGTLAGVEMGLSVAKVPHQKGGAQAAIDYLAQV
                     AAA"
     gene            4044..>4260
                     /locus_tag="VIR04_06815"
     CDS             4044..>4260
                     /locus_tag="VIR04_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9460492.1"
                     /translation="MSANQHVRAPALPNSASHGPSPFPLSPSGSEVVDRLTLPTSIAN
                     RLRELIIQGELQAGMRLNERALCDRLGV"
BASE COUNT         1045 a         1215 c         1087 g          913 t
ORIGIN      
        1 gcagcagcgc ggacgttcca gccgcatgcg ccgctgtttc gggggccacc acgccttcca
       61 gctcgcccgt gccgaatttg tccgcacgcg gcgaaaaacg ctgggcaata ccatagccca
      121 tgaaagaggc cgcggtagca cctccggggg taacgcccat ccagcatccg atcgcggtgc
      181 tccgaagcaa ggtcagccaa tatttgggca actttttcca ggtttgccac accactttca
      241 gatttatttt gccgctcttg ccttcaaacg ccaaaccctc ttccatgctg accaggattt
      301 cgccaatacc gaaaagcccg atcacgacca caaggaagtc aatcccgcga agcaaccccg
      361 gcaagccaaa cgtcatgcgc aattgaccgc ttatggtgtc cattccaatt tcagccaaac
      421 caaagcccag catcatggaa atgattgttt tgaacggcgc cttcctgtcc gtgccgacaa
      481 agctgcaaaa agtcagcagg aacacggcaa agaactcggc cggcccaaac cgcaaggcaa
      541 acttcgagac cattggcgcc aggaacgtga tcagcaatac cccaacaaag gcgccgacaa
      601 acgatccgga aaacgaagcc gtaagggcct cgccagccct gccttgctgc gccagcgggt
      661 aaccgtcaaa agtggtggcg accgatgaag gctcgcccgg gatattgaaa agaattgagg
      721 taatcgcacc ggcgaacaac gagccccaat aaatgcagga cagcataatg atcgccgaag
      781 tcggcgacat gccaaacgtg agcggcaaca aaatcgccac gccgttcggc ccccccaggc
      841 cgggcagaac gccgacaatc acgcctaata ccaggccgat catcatcatg cccaggttat
      901 accaggtcat tagtatagaa aagccatgaa acagggcgtc tatattttcc acgagtaaag
      961 cctccgttgt ccgttcttag taaccgaaca gggcttcgag cggccccttc ggaagcggca
     1021 ccataaactg gatctcgaac atccagaaca gcacgacgct aacacctaca ctgaccgcta
     1081 tggttttcag ccaactggtc ttgcccagca agcgcatgaa accgcctata aaaatagtcg
     1141 atgccacata gattccgatg agttgcacca ccagaacgta aagagcagta ggaatcagca
     1201 cgatcagcac cagcctgaac cggctccatg tcacgaaaac gacatgattg cggtgcttgc
     1261 caaacaagga tttcagcaat acggccaccg cggagatgca aagaatagcg ccaatatgaa
     1321 acggaaaata gccgggctga gggccttcgg agccccaacc cgagccaatc cggtaattat
     1381 cggccatcat gacggcgccg acgataaaaa tcacaacggc ggtcagggca tcgaccacac
     1441 tatacgtaaa gccgccaccc gaagggtttt cgggtgtttc gtgctgctcc atactacttc
     1501 tcctgtcgtg cgacttattt cttggcggcc agaccggctt cttgcatcac cgtcttgtga
     1561 taggcctcgt ctttagtcag gaagtctgtg aatgccttgc ccgttctaaa gtccgcatgg
     1621 agcgcttgct tggcaatata agccttccat tcgggagtct cgctgacttt cttcaacata
     1681 tcggcgtaaa aatcgacctg ctcctgggtt gtgccgggag gcaggaaaaa cgcgcgcaac
     1741 atttgatact gaacgtcgta acccgattct ttgcacgttg ggatgtcacc ccaggattga
     1801 tctttagtca ctttttcagt gtaagccatg cgttgttcgg agaatacgca taaagcacgc
     1861 aattgaccgg cgcgccactg agcaatgttt tcgctggggt tattgacatt ggaatcggtg
     1921 tgcttgccca ccaattgagt ggccgcttcg ccgccgcttt tatagggtac gtacgtaaag
     1981 tgcacccctg ccgctttgtc cagcgcaacg gtaatgattt gatcctcgcg cttggcgccc
     2041 gtgccgccca ttttgaactt tccgcgcggc gccgccttta ccgcattcaa gtaatccgca
     2101 gccgtcttgt atggcgtgtc ggcattaacc cataaaagga attcgtcttg cgcgatcatg
     2161 gccaccggat tcaggtcgcg ccagttaaat ggcaggttga cggcgatggg caaggtgtaa
     2221 atagccgaaa acgccaccat aagcttgtaa ggatctcctt tggagccttt ggcgtccatg
     2281 atgccttcag cgccgctggc gccaccttcg ttcagaacca gcgacgaggg cttcatcagt
     2341 ttatgcgtaa tgatgatgtt ctgaatggtg cgcgccattt gatcagaagc gccgcccgcc
     2401 ccggccggca caatgatctg cactgtgcga gtgggttccc acttttcggc ctgtgccggc
     2461 gcagccaggc aagccatcat ggctagcgac aaaggcagca aagccgcttt cttcagcgac
     2521 gtaatattca gatgtttcat ggttgtcttc tccattggaa attaataccg ggaatccgga
     2581 ccgtattgct tttacacaat attgcgtaat gtagcagcct tttaagcggc cgcgacctgc
     2641 gccaggtagt caatggccgc ctgtgctcca cccttctggt gtggcacttt cgcgacggaa
     2701 agccccatct cgaccccggc cagcgtgccg cataagctca gatcattcag atcgcccagg
     2761 tggccgatac gaaacaccgt gtctttcagt ttgctcaaac cttgacccag cgacatattg
     2821 aaatgctcga gcacaacctt gcggaacgca tcggcgctat gtcccttggg cattatcacg
     2881 gcagtcaggg ccgggctgta ttcggcgggg ttggcacaca gaatttccag gccccatgtc
     2941 cgtaccgcgc gccgagtcgc ttcgccgtga cgaatatgac gggcaaacac gttgtccaga
     3001 ccttcttcaa ataactgctc cagcgcttca tgcaaaccat acaacagatt ggtggccggc
     3061 gtataaggga aataacccgt agggttgatg tcaagcatgg ggtgccaatc ccaatacgag
     3121 cggggcagct tcgatgtttg cgatgccgcc agcgcctttg cgctaatcgc attaaaagac
     3181 aaacctggcg ggagcatcag acccttctgt gatcccgaga ccgttacgtc aacgccccat
     3241 tcatcatgcc gataatcaac cgaggccaac gatgaaatgg tgtcgaccat aaacaacgcg
     3301 gggtgattag cgcgatcaat ggcgtggcgc acagccggaa tgttgctggt cacgcccgtt
     3361 gcggtttcgt tatgcacaat gcaaacagcc ttgatggcgt gttctttgtc gctggtcagg
     3421 cgcgcctcga tcgttgcagc atcggcgccg cggcgccagt cgccgggaag aaattcaact
     3481 ttgaggccca gcttgacggc catttttttc cataaagcgg caaaatgccc ggtctcggcc
     3541 atcaacacca ggtcgcccgg cgataaagta ttgaccaggg ccgcctccca tgcgcccgtc
     3601 cctgaagcag ggtaaataac aacgtcgccc tttgtcttga acactttttt catgcccgcc
     3661 aggaccgttt tgccgatctc tccgaattcc gggccgcggt gatcaatcgt cgcgttgtca
     3721 atggcccgca atacacgatc aggaacgttg gtggggcctg gaatctgcaa aaaatgacgg
     3781 ccagacgggt gggaattaaa cgttatcatg ccagaaccct atagaaatgg tggtatacaa
     3841 aatacaataa cagatgatat taagcgtgtc tgaacaagac tgcaagaaaa ataaatgttg
     3901 tgccgcagca tgagttcgaa acgcagaaag gctatgctcc tatattccga tgaaaacaat
     3961 ggattgtttt tctgtatctt ttcctttaaa gttagccaac tgcgaatgat ttacaacgat
     4021 cccgattggt atacaaagaa aaaatgagtg ctaatcaaca tgtacgtgct ccggccctgc
     4081 caaattcggc gtcgcacggg cccagcccat ttcccctgtc gccatccggc agcgaagtgg
     4141 tggacaggct cacactgccc acgtcaatcg caaatcgctt gcgcgaactg attatccagg
     4201 gcgaattaca ggcgggaatg cgattgaatg aacgcgcctt gtgcgatcgg ctgggtgtat
//
LOCUS       DATCXU010000306         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1168337,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000306 DATCXU010000000
VERSION     DATCXU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168337"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VIR04_06820"
     CDS             complement(<1..697)
                     /locus_tag="VIR04_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9460493.1"
                     /translation="MKTVFAACVVIAAMSAIAHAQSLNLAGTWQGVLRLGSEQHQILK
                     ISPAQGGGWNGNLYWPDNAQAADTTIPHPITEISLRGREIQFSIEGAHYRGAISQDGK
                     SMSGELKQGDIALPFNLTRASSSTAWPIDPSKHTVHFVNVDKGVRLEIVDWGGSGPPL
                     IFLPGLGFTAHEFDGLAPDFVSTNHVYAITRRGFGQSSKPAPTDKNYSADRLGDDIIA
                     VMNALKLNRAIVAG"
     gene            complement(875..1945)
                     /locus_tag="VIR04_06825"
     CDS             complement(875..1945)
                     /locus_tag="VIR04_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460494.1"
                     /translation="MIRPFLLCGLAALTLLGGCGRKAAPPTLFQWEDYADPPFLADYI
                     KAYHEKPNVVPFGDEDEAFAKLQRGFRPDVMGPCYYEFPRWKEAGLLQPIDTTKLKNW
                     NKLSPTLRHLPGIDAGNGKVWFVPHYWGNTSLTIRTDLAPEYAKSQSWNILFDPKYKG
                     RVSVLDGVDDTVPFIARMIGVDAYTMSDADWVKVQAKLKALMPQLRFVSSDDTALAQG
                     LASGELVAAMSWRETFAALRREHKPVAYLEPPGGIFTYVCGLVMHKNPSDEAKALALI
                     DSALSDEAAVYTIEHIAGGPANLSAMDKVPDQVFADLGIPRDLESFLRNGIFQRRLKN
                     KEKIIEAWTDLRAGVAAPVAKN"
BASE COUNT          420 a          606 c          616 g          453 t
ORIGIN      
        1 gccccgcgac aattgcgcgg ttcagcttga gtgcattcat cacggcaata atgtcgtcgc
       61 caagtcgatc ggcagaataa tttttgtcgg tcggggccgg cttactggat tgaccaaagc
      121 cgcgccgagt aatggcatag acatgattag tggagacaaa atcaggggcc aaaccatcaa
      181 attcatgagc cgtaaaaccc aagcccggca aaaaaatgag cggtgggccg ctcccgcccc
      241 aatccacgat ttctaagcgc acccctttat ctacgtttac aaagtgaaca gtgtgcttcg
      301 atggatcaat cggccacgcc gtgctggaac ttgcgcgtgt caggttgaac ggtaacgcaa
      361 tgtcgccctg cttaagttcg cccgacatag atttgccgtc ttgacttatc gcacctctgt
      421 aatgagcgcc ctcaatcgaa aactgaattt cacgtccacg cagtgaaatt tcggtgatcg
      481 gatgaggtat ggtggtgtcc gctgcttgcg cgttatcagg ccaataaagg tttccattcc
      541 atccaccacc ttgtgccggg gatattttca aaatttgatg ttgttcgctt cctaggcgca
      601 gcactccctg ccaggttcca gcaaggttta atgattgagc atgtgcgatg gctgacattg
      661 ctgcaatcac aacgcaggct gcgaaaactg tcttcattga gccatctcct gcttcaatgc
      721 ctagagattg aactgatgaa gtcgcgcgat tatgagtagt ttgtcgcaag ggttagccaa
      781 ccccgccata agctattggt atgtattggg aggaactttg cggattagcc aatttatccg
      841 ttgatttcac tcagttggta cacccgcgcg cgccttaatt tttcgcgacc ggcgccgcga
      901 cgcccgcgcg caggtccgtc cacgcttcga tgattttttc cttgttcttc agccgccgct
      961 ggaagatgcc gttcctgagg aagctctcca ggtcgcgcgg aatgcccaga tcagcgaata
     1021 cctgatccgg caccttgtcc atggcgctga ggttggcggg gccgcccgcg atatgttcga
     1081 tggtatagac cgccgcttca tccgagagcg cggaatcgat cagcgccagc gccttggctt
     1141 cgtccgacgg gttcttgtgc atcaccaggc cgcagacata ggtgaagatg ccgcccggcg
     1201 gctccagata ggcgacaggc ttgtgctcgc ggcgcagggc ggcgaaagtt tcgcgccagc
     1261 tcatcgccgc caccagttcg cccgaggcca ggccctgcgc cagcgccgtg tcgtcggagg
     1321 agacgaagcg cagctgcggc atcagcgctt tcaattttgc ctgcactttc acccagtcgg
     1381 cgtcggacat ggtgtaggcg tcgacaccga tcattctggc gatgaagggc acggtgtcgt
     1441 cgacgccgtc cagcaccgag acgcggccct tgtatttggg gtcgaacagg atgttccagc
     1501 tttgcgactt ggcgtattcc ggcgccaggt cggtgcggat ggtgagcgag gtgttgcccc
     1561 aataatgcgg cacgaaccag accttgccat tgccggcatc gataccgggc aggtggcgca
     1621 gcgtcggcga gagcttgttc cagttcttca gcttcgttgt atcgatgggc tgcagcagcc
     1681 cggcttcctt ccagcgggga aattcgtaat agcaggggcc catcacgtcg ggcctgaagc
     1741 cgcgttgcaa cttggcgaag gcttcgtcct cgtcgccgaa gggaacgacg ttgggctttt
     1801 cgtgataggc cttgatgtaa tccgccagga acgggggatc ggcataatcc tcccactgga
     1861 acagggtcgg cggcgccgcc ttgcggccgc aaccgcccag cagcgtcagc gccgccagcc
     1921 cgcacagaag aaacggccgg atcaccacaa ggagaagcgg ctgacgcgcg gatccccacc
     1981 gaagccgata ttgaggctga tgccggcgct ggtttcgccg cccagttcgc cctgccgcag
     2041 cgagatgttg ctgtggcggg caccaagctc gatctcgaag ccggcggcag gcgtg
//
LOCUS       DATCXU010000307         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_708834,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000307 DATCXU010000000
VERSION     DATCXU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708834"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VIR04_06830"
     CDS             complement(<1..670)
                     /locus_tag="VIR04_06830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY9460495.1"
                     /translation="MYRPIIAITMGDAAGVGPEIIMKSLADEHVYERCRPLVVGDTIR
                     LREAGVIVGSSLEVRSVQGIQDAGFKSGVVDCIDLNLIPPKHPFGKLSPLCGDAAYRY
                     IERAVQLANGKEIDAICTAPLNKEALHAGGHHYPGHTEMLAHLTGTPEVSMMLVADKL
                     RVIHVTTHIGLLDAINKIEPGLVERTIARGYDTLVRAGMEAPRIGVCAINPHAGEHGL
                     FGHGE"
     gene            complement(696..1985)
                     /locus_tag="VIR04_06835"
     CDS             complement(696..1985)
                     /locus_tag="VIR04_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEY9460496.1"
                     /translation="MTQKINQILIIADDLSGAADCTAGAVRAGLLTQVSLGHALAENA
                     VAPQIQVWSIDTDTRRLSPADAAKINAEILSRCRKPGQLLYKKIDSTLRGNFAAELLP
                     MLAPSEMAVVAPAFPDAGRSTRNGRQYVDGVALEETEIWRHEGISGIADIQGMLCAQG
                     IRTALLRLDQVRAGHPQLRKNMEDCIAQGMQAVVCDAETNEDLQRIAQASIALSPSIF
                     WVGSAGLANQLPEAAGLIGHAQPPGTGVNGVILTAVGSMSAISRQQMHHLSVSRAMMR
                     IDIRTDMLSDGEHHRDWHKQQSRLGAAFENGDDVLLAISDARYLDIHQGWMLCQALAR
                     LVSPFSSCIGGMISTGGETTHALLSAFKSTGLHIIREIQPGIPLSVTLGARTFPIISK
                     AGAFGSAQTLVYCYDVLHRMRAESTHGANLKNEARTL"
     gene            complement(2129..2500)
                     /locus_tag="VIR04_06840"
     CDS             complement(2129..2500)
                     /locus_tag="VIR04_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY9460497.1"
                     /translation="MKNNPVGWFEIYVQDMERAKPFYEKVFGVSLAKLDNPEIEMWAF
                     PMEMEHYGAPGALVRMPGFSSGQNSVLVYFSCEDCASEEAKVERCGGKVVKKKFSIGE
                     YGHISLVTDTEGNMIGLHSMQ"
     gene            complement(2621..2923)
                     /locus_tag="VIR04_06845"
                     /pseudo
     CDS             complement(2621..2923)
                     /locus_tag="VIR04_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
BASE COUNT          589 a          844 c          828 g          678 t
ORIGIN      
        1 cttcgccatg gccaaacaag ccgtgttcac ccgcatgagg attaatcgcg cagaccccta
       61 tgcgcggcgc ttccatcccc gcgcgcacca gggtgtcgta tcctcgcgca atagtccgtt
      121 ccacaagccc gggttcgatc ttgtttattg cgtccagcag gccaatatgg gtagtgacgt
      181 gaatgacccg gagtttgtcg gcgaccagca tcatcgacac ttccggtgtg ccggtcaaat
      241 gcgcgagcat ttctgtatgg cccgggtagt ggtggccgcc cgcatgcagc gcttccttgt
      301 ttaaaggggc tgtgcaaatc gcgtcaatct cttttccgtt tgccagttgc acggcccgtt
      361 cgatgtagcg gtaggcggca tcgccgcata gcggggatag cttgccgaat gggtgtttgg
      421 gtggaatcag gttaaggtca atacaatcca cgacgccgct tttgaatccg gcgtcctgaa
      481 ttccctgcac tgagcgcact tcgagcgagc ttccgacaat gacgcccgcc tcgcgcagac
      541 ggatagtgtc gccgacgacc aagggccggc agcgttcata cacgtgttcg tcggccaggc
      601 ttttcataat aatttcgggg ccgacgcccg cagcatctcc catcgtgatc gcgataatgg
      661 gacggtacat agtagtttcc tatagtgatt gatttttaaa gcgtgcgcgc ttcatttttc
      721 agattggcgc catgcgtgga ctcagcccgc attcgatgca gcacatcgta acaatacacc
      781 agtgtctggg cagagccgaa tgcgccggcc ttggagataa ttggaaaagt gcgcgcgccg
      841 agcgttacag atagggggat ccccggctgg atctcgcgaa ttatgtgcag tcctgtcgac
      901 ttgaacgctg acagaagcgc gtgtgtcgtt tcaccgcccg tcgaaatcat gccgccaata
      961 caggatgaaa atggcgatac caggcgcgcc agtgcctggc acaacatcca accttgatgt
     1021 atgtcgaggt atcgggcgtc ggagatcgcc aacaacacgt cgtcgccatt ttcaaatgcg
     1081 gcgccaaggc ggctttgttg tttgtgccaa tcgcggtgat gttctccgtc actgagcatg
     1141 tccgtgcgaa tatcgatacg catcatcgct ctggaaacgg acaagtggtg catttgttgg
     1201 cgcgagatgg cggacatgct gcccaccgcg gtcaaaatga cgccgttgac cccggtgcct
     1261 ggcggttgcg cgtgtccgat aaggccggcc gcctcgggca actgattggc caggcccgca
     1321 gagccaaccc agaatattga tggcgaaagt gcgatcgacg cttgcgcaat tcgttgcaga
     1381 tcttcattgg tttcggcatc gcatacgaca gcctgcatgc cttgcgctat gcaatcttcc
     1441 atgttcttgc gcaactgtgg atggccggcg cgcacctgat cgagacgaag taaggcggtg
     1501 cgaattccct gcgcgcagag catcccttga atgtccgcga ttccggagat gccttcatgg
     1561 cgccagattt ccgtttcttc cagcgcaact ccgtcaacat attggcgtcc gttgcgcgtg
     1621 ctgcggccag cgtccgggaa tgcgggcgcc accacagcca tttccgaggg cgccagcatg
     1681 ggcagcaatt cggccgcgaa atttcctcgc aacgttgagt cgattttctt atacaacagt
     1741 tgtcccggct tgcgacagcg gctgagtatt tcagcattaa ttttggcggc gtcggcgggg
     1801 gacaacctgc gtgtgtcggt atcgatcgac caaacctgaa tctggggcgc gacagcgttt
     1861 tcggccagtg cgtggcccaa tgaaacttgc gtaagcaatc cggcccgaac cgctccggcg
     1921 gtgcagtcgg cagctccgga cagatcgtcc gcaatgatta gtatttgatt tattttctgg
     1981 gtcaccgcgt agcgccttca tgggcgtcgt cgttattagg gggattgaaa gcacgaacgg
     2041 ttttacgcgc gataggcgca ttttaccttg ccttccgtgg ctttacaaag tgcttgcgcg
     2101 cgcagtggcc gcgcaagcgc ttgccgggct actgcatcga atgcaggccg atcatgttgc
     2161 cttcggtgtc ggttaccagc gaaatgtggc catattcacc gatcgagaat tttttcttta
     2221 ccaccttgcc gccgcaacgc tccaccttgg cttcttccga cgcacaatct tcacaactga
     2281 agtaaaccaa gacactgttt tggcccgaag aaaagccagg catccggacc aaagcgcccg
     2341 gagcgccgta atgctccatt tccatcggga aggcccacat ttctatttcg ggattgtcca
     2401 gctttgcgag ggaaacgcca aatactttct cgtagaaggg tttcgcgcgt tccatgtctt
     2461 gcacataaat ttcgaaccag ccaaccggat tgttcttcat ggcgactcct ttgataacgc
     2521 gcgcaaaatc gatatccgga cttggcgcgc cttcacccgt ttcaagatac tgctgcaggc
     2581 tgacaaggta atagccccag cccgtcgttg tgaccgcata ggcttcgtca gcttctttga
     2641 agtcgcggtg cgcgaaggag atcactgtgt cgtccccgtc cacccgcaga tcgaacataa
     2701 ttgtcgtacc gacccattcg ggacggaacg aagcgactgt tttccagact acgcgggttg
     2761 gcggctccag atcttcaata cggactgtcg tggcgaacac gcccttgtat gagaagcgaa
     2821 acttccctgt tcctccgact tgcgaatcca gctcggcgtc gcgcgtccac catgcgcgga
     2881 tgccttcgac ggtggtgagc gcctggtagg cccgcttggg caatgcccgg gccctgacg
//
LOCUS       DATCXU010000308         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_481215,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000308 DATCXU010000000
VERSION     DATCXU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481215"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VIR04_06850"
     CDS             <1..321
                     /locus_tag="VIR04_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY9460498.1"
                     /translation="LALGVANGLDPKVLSDIIAKSSGRNWATELYNPWPGIMAHAPAS
                     NGYSGGFGVDLMLKDLGLAAESALGVRATIPLGELARNLYSLHSAQGNGLLDFSSILN
                     LIKK"
     gene            complement(333..989)
                     /locus_tag="VIR04_06855"
     CDS             complement(333..989)
                     /locus_tag="VIR04_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY9460499.1"
                     /translation="MPAKIVSHAPLRHRLPTDERKREIVSTVVALAYKQGPDTITTQA
                     IADRIGITQGAIFRHFPDKESIWLAVFDWLSKSLGAALTAAIAGVNSPLAKIERMFIA
                     HVAFIAAHPGVARIMFHELQYPGNTPVRAQVRTLISAYRKRLASLFIQARAQNELPAG
                     LNVTLAPVLFIGAVQGLVIQASLAGDETEMVEQARQLLPLLLHGYCGRDPKPHSSRPA
                     "
     gene            1081..1686
                     /locus_tag="VIR04_06860"
     CDS             1081..1686
                     /locus_tag="VIR04_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460500.1"
                     /translation="MSYPDFFDKAPRITMQDPLAAFLGAAEDGIMEYGYADAVKLAGH
                     SCPTVASTYLMARSAFATLYPGELPKRGDIQVDLPDERQDGVAGVVANVLSLLTGATQ
                     DTGFKGIGGRFDRRNLLSFGVPVSGQIRFTRLDTEDSVTVSARLNRVPGDPRVAGLMP
                     RCISNTATDYEATLFQTLWQERVRKLLIEHADDPDVIVVQH"
     gene            complement(1683..>2070)
                     /locus_tag="VIR04_06865"
     CDS             complement(1683..>2070)
                     /locus_tag="VIR04_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HEY9460501.1"
                     /translation="AATQDLFIPQTMNLDLIDGVSFNKGCYPGQEVVARSHYRGKIKR
                     RTAYGVIQGHARPQDSELAGADVFDARSPGRPCGRVINAAQTDRTHLLFEVQLADLGT
                     ADFRIDRANGPEIALQTLPYEITGIS"
BASE COUNT          419 a          567 c          612 g          472 t
ORIGIN      
        1 cttgccctgg gggtggccaa tggtcttgat ccaaaagtcc tctcggacat tatcgccaaa
       61 agctcgggcc gcaactgggc taccgaactg tacaacccat ggccgggcat catggcgcat
      121 gcgccggcgt ccaacggcta ctcaggcggc tttggagttg atttaatgct taaggatctt
      181 gggctggcgg ctgaatcggc gcttggcgtt cgcgccacca ttcctttagg cgaattggcg
      241 cgcaacctgt attcactgca cagcgctcaa ggcaatggct tgctcgattt ctccagcata
      301 ttgaatttga tcaaaaagtg aacaagcctg atttacgccg ggcgtgaaga gtgcggcttt
      361 ggatcgcgcc cgcaatagcc atgcagcaat agtggcagca attggcgcgc ttgttccacc
      421 atttctgttt cgtcgcctgc cagagatgcc tggataacca gtccttgcac agcgccgatg
      481 aataataccg gcgccagggt tacgtttaaa ccggcgggaa gttcgttttg cgcccgtgct
      541 tgtataaata gcgaagccag acgtttgcga taggcgctga tgagggtgcg cacttgcgct
      601 cgcacggggg tattgcccgg atactgcagt tcatgaaaca taatgcgcgc cacgccaggg
      661 tgggccgcaa tgaatgccac gtgcgcaatg aacattcgtt caatcttggc cagaggcgag
      721 ttcactccgg caatggcggc ggttaacgcg gcgcccagcg atttgctgag ccagtcgaac
      781 acggccagcc atatcgattc tttgtcgggg aagtgccgaa agatcgcgcc ttgagtaatg
      841 ccgatacgat cggcaatggc ctgtgtcgtg atcgtatcgg gaccttgttt gtacgccagc
      901 gccacgacgg tgctgacgat ctcccgtttt cgctcgtctg tgggcagacg gtggcgcagg
      961 ggagcatgag agacaatttt ggcgggcata aagtcagttg cttataagta agtaattaat
     1021 tactatatta cgagagcgtc aggctctttt caagcccggc caatccaagg agaagcagta
     1081 atgagttacc ccgatttttt tgacaaggcg ccgcggatca ccatgcagga tccgctcgca
     1141 gcgtttctcg gcgcggccga agatgggatt atggagtatg gctatgccga cgcggtaaag
     1201 ctggcgggcc attcctgccc cactgtggca tcgacgtatt tgatggcgcg ttcggccttt
     1261 gccactcttt atccaggcga gttgcccaaa cgcggcgaca ttcaggttga tcttcccgac
     1321 gagcgccagg acggcgttgc cggagtggtc gccaatgtcc tcagcctgct gacgggcgcg
     1381 acccaggata ccggtttcaa aggcataggc ggtcgatttg atcgccgcaa cttgttgtcc
     1441 tttggcgtgc ctgtttcggg tcaaatacga tttacccggc tggataccga ggattccgtc
     1501 acggtgtccg cgcgccttaa tcgcgtgccg ggcgatccgc gcgtcgcggg actcatgccg
     1561 cgttgcatct cgaatacggc cacggattac gaggcaacgc ttttccagac attgtggcag
     1621 gaacgcgtgc gcaaactgct tattgaacac gccgacgatc ccgacgtgat cgtggtccag
     1681 cactaagaaa ttcctgtgat ttcatagggc aaggtttgta atgcgatttc ggggccattt
     1741 gcccggtcaa ttcggaaatc ggccgttccc aggtcggcca attgcacttc gaacaggaga
     1801 tgcgttcgat cggtctgggc cgcgttaatg acacgcccgc agggcctgcc cggagaacgg
     1861 gcatcgaaaa catcggctcc ggcaagctcg ctgtcctgag ggcgcgcatg tccctgaatc
     1921 acgccgtacg ccgtgcgccg cttgatcttt ccgcggtaat ggctgcgggc gacgacttcc
     1981 tggcccgggt aacacccttt attgaagctg accccgtcaa tcagatccag gttcattgtc
     2041 tgagggataa acaggtcttg tgtggctgcc
//
LOCUS       DATCXU010000309        14846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_822454,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000309 DATCXU010000000
VERSION     DATCXU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822454"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VIR04_06870"
     CDS             <1..215
                     /locus_tag="VIR04_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460502.1"
                     /translation="QFILLMLASSVVSVISADDLTSVAANLVSQTFRSFEIYIVITVI
                     YLLLTFIFSYLFRLVFRWGLDYPDGR"
     gene            215..874
                     /locus_tag="VIR04_06875"
     CDS             215..874
                     /locus_tag="VIR04_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460503.1"
                     /translation="MRTFGFSEFLFILDAARWTISLSLIAFVGGGVVGLAVALSRVSH
                     HKALRAWSVAFIQVFRGTPLLLQLFLIFFGAPVLGFDINPWLAAGIALTLNSGAFLGE
                     IWRGCIQAIARGQWEAAEALGLSFRWKMQDVILPQALRIAVPPTVGYLVQIVKGTSLA
                     AIIGFTELTRAGQIMNNATFQPMLVFAVVASIYFFLCWPLSLLAAYLERRLAIQMTSQ
                     I"
     gene            925..1731
                     /locus_tag="VIR04_06880"
     CDS             925..1731
                     /locus_tag="VIR04_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460504.1"
                     /translation="MLLIKQIRLLASAAALAACGVLALGASAATAQTVDQIKAKGEIT
                     VGMLVDFPPYGIVNTENKPDGYDADVARLLGKDLGVKVNLVPVTGPNRIPFLLTNKVD
                     VLIASLAITPARAEQVQFSKPYSAAQIVLFGAKDAKISSAKDLSGLRVGVARASTQDI
                     AVTKIAPKDANIRRFDDDASAMQALLSGQVDAIGCSTTVAAAISKRTPDRYAPKFVLL
                     QQEMAVAMRKGDPELLKTVNGFVEKNIKNGELSKLYQKWLGSPLPALKAS"
     gene            1732..2550
                     /locus_tag="VIR04_06885"
     CDS             1732..2550
                     /locus_tag="VIR04_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9460505.1"
                     /translation="MDDDSGLLMTNKNGSIDAHGRDRPASGDAAIIEIDAANKWYGGF
                     QALTDVNLRVQTGERIVICGPSGSGKSTLIRCINGLEKVQKGRIVVDGIDLTAGSRNI
                     EAVRQEVGMVFQQFNLFPHMTILQNCMLAPIRSRRVSKQQAEALAMRFLDRVRIADQA
                     KKYPSQLSGGQQQRVAIARALCMTPKVMLFDEPTSALDPEMVKEVLDTMIGLADDGMT
                     MLCVTHEMGFARKVANRVIFMDQGKIIEQAPPDQFFNNPQHEKTRNFLGQILNS"
     gene            2602..3546
                     /locus_tag="VIR04_06890"
     CDS             2602..3546
                     /locus_tag="VIR04_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY9460506.1"
                     /translation="MAGPLDVVTFGEAMLLLVADQPGPLERVEMFHKRTAGAETNVAI
                     GLARLGLKVGWASRLGADMMGRYLLAEMRREGIDCSHVVCDPHQYTGLQFKGRVDDGS
                     DPPVQYHRQGSAASQMQPADIDQAWLCSARHLHCTGVFAAISSNCFQVSVNAAALMRA
                     AGRTISFDPNIRPTLWSSVDFMRQSINQLAFASDWVFPGLGEGRILTGQDTPEAIARF
                     YREHGAKLVAVKLGADGAYFDGPDGCGYVDSYPVKNVVDTVGAGDGFAVGVISALLDG
                     CELPDAVRRGAWIGARAVQVLGDTEGLPTRAQLEEAGL"
     gene            complement(3565..4200)
                     /gene="eda"
                     /locus_tag="VIR04_06895"
     CDS             complement(3565..4200)
                     /gene="eda"
                     /locus_tag="VIR04_06895"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY9460507.1"
                     /translation="MNALELLQKSPVMPVIVIKDIDSAVALAQALVRGGVRSLEITLR
                     SAAALDAIRLIASAVPDALVGVGTVRDARQLDAAVKAGAQFAVSPGLTPGIAKAASAA
                     SIPFLPGVASASESMIAADHGFTVQKLFPAEAIGGVALLKALYGPLPDIVFCPTGGIN
                     AANAKKYLDLPNVKCVGGSWLTPGAAVAARQWDAITQLALQACSLASMPAP"
     gene            complement(4214..4588)
                     /locus_tag="VIR04_06900"
     CDS             complement(4214..4588)
                     /locus_tag="VIR04_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY9460508.1"
                     /translation="MTDRHLIIIAALTLLAGVAAGAFGSHGLKRVLEPELLAVWQTAV
                     FYQLIHGLGLLAIAVLGARFGSPLLSHAGTVMFAGMLLFSGSLYILALTGIKWLGAIT
                     PIGGLAFIAAWAMMALAVYRSN"
     gene            4754..5758
                     /locus_tag="VIR04_06905"
     CDS             4754..5758
                     /locus_tag="VIR04_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY9460509.1"
                     /translation="MSTVATVSGGAEKSIVASVLYRPGQAPVNVPIDKLAQTVDAEKQ
                     LLWIGLKEPPVELLEQIGVQLRFTPRALEEISESHRRPKIIEYQQFTLIVAITIEVDN
                     MRPIFGETQLLIGEGFLITIRRGATATHIDLRQHLESYPEFLERGSDYVASTLLDLMV
                     DRYATALKQLETGVENMEQRFLLRGLANADVRRLYRQRRDLLRIQGAISPMAEICRRL
                     SHVEMGHIDANCQGYYGEVADRVVRLNELITSLREALAFAFEAGLMIGQSHQTDITKK
                     LAAWAAILAVPTAVAGIYGMNFKYMPELGWKYGYALIMAAMALACGVLFWKFKKAGWL
                     "
     gene            5785..7452
                     /locus_tag="VIR04_06910"
     CDS             5785..7452
                     /locus_tag="VIR04_06910"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY9460510.1"
                     /translation="MGQVTHQVTNQVPDLSDYNLYLSDRALLEGVRREGAGWHEQALI
                     DYGAQLGLRESFSAAHDANRYKPELEAFDRHGVRVDRVHFHPAWRQFMRMAFAQRMHC
                     SAWREPCVGAHVARAATYLLHGQVEAGSLCPVTMTSAAIPVLQREPLFESIKTLLFSD
                     RYDGRDLPLDGKAGMTIGMGMTEKQGGSDLRSNTTHAEPVAAAGRGATYRLTGHKWFF
                     SSPDSDAHLVLARHKQIFSCFYVPRWRAGGDKNAVHIQRLKDKVGNSSNASAEVEFID
                     AQGVLVGEEGRGIATLVEMAMYTRLDCILGSAALLRQALVQALHHANRRFVFGRALIV
                     QPLMQSVLTDLALESEAATVLALRLARALDDPGDSLNQAYRRVLTPAAKFWVCKRGIE
                     AVAECMEVWGGNGYIEEAPMARLYRESPVNSIWEGSGNVMCLDVLRAINRHPEMADVL
                     HTTLLRDCAGEPLLKERMARLSDVLSASGPTLEAAGRYVAQELVLLSQASLLLQCAPQ
                     AVADAFIQTRFAGRGGRVFGAGVPSALAGPILERAWSESSVDPVQSR"
     gene            7469..8053
                     /locus_tag="VIR04_06915"
     CDS             7469..8053
                     /locus_tag="VIR04_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY9460511.1"
                     /translation="MIPRFGMCAVRALLFAAGGAIAGFGLGAMAAPTHHGGSAHDAYT
                     LAGRVVHVADGDTFTLLVEGREERIRLASIDAPETTHGRERPGQPFGQVSRDALARLV
                     AGKTLSVICFERDRYERSVCNVPLSDGSTANQKQVADGVAWANMEGHGKYMRDPKIPE
                     LEAQARHSRLGLWRSPNAVRPWVWRYQCWKQRQC"
     gene            8112..10355
                     /locus_tag="VIR04_06920"
     CDS             8112..10355
                     /locus_tag="VIR04_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460512.1"
                     /translation="MISSAAGCRWLACLLALGLLAGCSRSLVNSPYARGDEKQNVLYT
                     AFVQRSPKFLDPASSYSTDETPFTYSIYEPLYGYDYLRRPYTLVPRAAAAVAEPVYLD
                     RAGRRLPNDVAGDQVDVSVYDIKIKPGILFAPHPAFARNAAGQYRYWPMAANDLEHAF
                     SIGDFSETGTRELTASDYVYAFRRLASPRVISPIYGVMAKHIVGMRAYGDRLKARDQA
                     VRAQGGSASDQAWLDLREQGFDGVQALDAHTLRIKVIGKYPQFKYWLAMTFTAPVPWE
                     ADRFYHQPGMAEHNLSLNTWPVGTGPYMLTESIQNRRHVLSRNPNFRGEPYPCHGEPA
                     DQAAGLLKDCGKPTPFIDRIVFSLEKESVPLLGKFLQGYYDIPQADRGDYGVAMRVAA
                     GDSAEKASLYRSHGLQLLASTEPQISYFGFNWLDPVVGRGDTPAQQEKNRKLRQAISI
                     AFNWEQYVSIFENDQAQVAYGPVPPGILGYQAAPQGIDPYVYTMENGRPVRRSLDQAK
                     ELLTQAGYPGGRNAQTGAPLILHFDSAAGMGSNATFDWMRRQLKALNIQLEIRATDYN
                     RFQDKMRNGSAQMFMWGWVADYPDAENFLFLLYGPNAKASKGGENASNYHNPEFDRLF
                     EQMRFLDDGPAKEQIVHKMVAIVQADAPWMFGFYPKSGGAYQSWVSNAKPTQMVRDTL
                     QYYRIDPALRAKKIAQWNAPVWWPLWLLAVLALLGLYPAYRVIKRREGATALGSQVTV
                     LTHSRRP"
     gene            10352..11329
                     /locus_tag="VIR04_06925"
     CDS             10352..11329
                     /locus_tag="VIR04_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460513.1"
                     /translation="MIRYIVRRLLYGVLVLIGVNLLTFVLFFAVNTPDDMARLSIGGQ
                     RVSQSAIDKWKIERGYDKPLFFNADAAGAAKWTDTIFYARSAPLLKFDFGFSDDGHDI
                     GHQITQRMWPSLALALPTFVLGLGICVAFALLLVFFKGTPLDFAGVVICVVLLSISAL
                     FYIIAGQWFFSKMLRWAPYSGYIDGWGSLRFLIVPVLVGIVSGMGGQARFYRTLFLEE
                     ISKDYVRTARAKGLSERIVLFRHVLRNALLPILTGTVSAIPLLFMGSLISESFFGIPG
                     LGSYTIDAINAQDFSVVRAMVFLGSVLYIVGLILADLSYTIADPRVRFE"
     gene            11334..12836
                     /locus_tag="VIR04_06930"
     CDS             11334..12836
                     /locus_tag="VIR04_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460514.1"
                     /translation="MPKFVFLWTDIFIFCLLLAVLLYALKARRSTALRAAWASVAHTP
                     SAMCSAVALSLFVLIGVLDSVHYRPVLPAVNSAGHSVRAIVPVYATAPRSLLDDVLSL
                     TRLVDPETTYSAPLAIRQFTKESEVVNGKPVRDYPRLKYAGANLAHDADRGADISRRL
                     VWGLLAGVLGSAASALTLTLAGLKKGRPFKRAAAAWWRGETRIPWRPMWITLSIIMLV
                     CAVVIALSTNYHVMGTDRTGNDVLWQALKSIRTALVIGTLTTIAMLPPALAFGISAGY
                     FKGRVDDVIQYIYTTLTSIPGVLLIAACVLMMQVYIDNNPGLFDTTAARADLRLFLLC
                     LILGLTGWAGLCRLLRAETLKLRELEYVQAARAFGVGHWRIMRRHLLPNVMHIVLITL
                     VLEFSGLVLYEAVLSYLGIGVDPSMNSFGSMINSARFEMSRDPMIWWNLLTAFTFMLA
                     LVLAANIFADAVQDAFDPRTRKFRPSRAPAGLRAPLVIPGSSDAATKDPL"
     gene            12833..14560
                     /locus_tag="VIR04_06935"
     CDS             12833..14560
                     /locus_tag="VIR04_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460515.1"
                     /translation="MIFPASDQIDKATNDGPSVLSVEDLSVRIASESGVIDAVKSLGM
                     AIARGQTFALVGESGCGKSMTALALLRLLPEAARTASGKIGLDGLDLNRLPEFRMRAI
                     RGRRIGIVFQEPSTSLNPVLSVGQQVIETLFAHTAYRGAAAIERATWWLKRVGIPNAA
                     ERLRDYPFQFSGGQKQRVMIAIALAAEPAVLIADEPTTALDVTVQAQILDLLADIQKE
                     TGLAILLITHDLAVVKNVADVVALMRAGEIIETSDASRFFSAPRHPYARELLAAIPTL
                     AKRGHSLRPHLPATPPPAVAIRTNINSVAGGSPDAASVLIVEHLSVSYQKRAGLFHRQ
                     TGSNKVVDDVSFQLYRGETLALLGESGCGKTTTAKALLRLLDKQAVVGGRASLGGQDL
                     LSAHGARLKRLRRIMQIVFQDPFASLDPRMLVGEILDEGVAALRPDHSRLDRQASVRN
                     LIDRVGLPRNTMDRYPHEFSGGQRQRIAIARALAVAPEVLICDEPTSALDVSVQAQIL
                     ELLRALQVEFNIAYLFITHNFGVVEYLSDRIAVMHEGKLVEIGETEPVLKAPKHAQTQ
                     RLIAAVPRL"
     gene            14635..>14846
                     /locus_tag="VIR04_06940"
     CDS             14635..>14846
                     /locus_tag="VIR04_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY9460516.1"
                     /translation="MSLKVFDLQCQHGHVFEGWFGSLDNYESQKARGLIACPICDSRQ
                     IVKKISAPRINVNHGRAPDPGAAPAQG"
BASE COUNT         2883 a         3886 c         4552 g         3525 t
ORIGIN      
        1 gtcaattcat actgcttatg ctggcgtcca gcgtggtctc ggtgatttcc gccgacgatt
       61 tgacgtccgt cgcggccaat ctcgtctccc agacatttcg cagttttgaa atttatattg
      121 tcatcacggt catctatttg ctgttgactt ttatattttc gtatctgttt cgcctggtgt
      181 tccggtgggg ccttgattac ccggatggcc gataatgcgc accttcggat tttcggaatt
      241 cctttttatt cttgacgcgg caaggtggac gatctcgctg tcgcttattg cgtttgtggg
      301 cggcggcgtg gtgggacttg ccgtcgcgtt atccagggtg tcgcatcaca aggcgctgcg
      361 ggcctggtcc gtggcgttta tacaggtgtt tcgcgggact ccactgctgc ttcagttgtt
      421 tctaatattt ttcggcgccc ccgtgcttgg tttcgacatc aacccatggt tggcggcggg
      481 tattgcgcta accttgaata gcggtgcgtt tctgggcgaa atatggcgcg gctgcattca
      541 ggctattgcc cggggccaat gggaagcggc cgaggcgctg gggttgtcct tccgctggaa
      601 aatgcaagat gtgattctgc ctcaggccct gcggattgct gtgccgccca cggtgggtta
      661 tctggtccag atcgtcaagg gtacgtcgct tgcggcaatc attggtttca cagaattgac
      721 gcgtgcgggc caaattatga acaatgcaac ttttcagccg atgctggttt ttgcggtcgt
      781 tgcaagcatt tatttcttct tgtgctggcc cttgtctttg ctggccgcct atctggaacg
      841 gcgccttgcc attcagatga caagccagat ctaacatggg tatattttca aaccggtggt
      901 gtcctttatt tttggagaca agtcatgctt ctaattaaac agataaggtt acttgcaagc
      961 gcggcggcgt tagccgcttg tggtgtattg gcgctgggcg cttccgccgc aacggcgcag
     1021 acagtggatc agatcaaggc caagggtgaa atcactgtgg ggatgctggt tgattttcct
     1081 ccatacggca ttgtgaacac tgaaaacaag cctgacgggt acgatgcgga tgtggccagg
     1141 ttgctgggca aagacctggg tgtcaaggtt aatcttgtgc cggttacggg ccccaatcgc
     1201 attccctttt tattgacgaa caaggttgat gtgctgattg cttcgctggc cattaccccg
     1261 gcccgcgccg agcaggtcca gttttcaaag ccttattcgg cggcccaaat agtgctcttt
     1321 ggcgctaaag acgcaaaaat ctcctctgcc aaagacttgt cgggcttgcg cgttggggtg
     1381 gcgcgtgcca gcactcagga catcgcggtg accaagattg cgccgaaaga cgccaatatc
     1441 aggcgcttcg acgacgatgc ctcggccatg caggccttgc tttcgggcca ggtcgacgcc
     1501 atcgggtgtt caacgacggt cgcggccgcc atctccaagc gcactcccga tcgctacgcc
     1561 ccgaaattcg ttctcctgca gcaggaaatg gcggttgcaa tgcgcaaagg cgatcccgaa
     1621 ttgctgaaga cggtgaatgg ctttgtcgaa aaaaacatca aaaatggcga gttaagcaag
     1681 ttgtaccaaa agtggctggg ctcgcccttg ccggcattga aggcatcatg attggacgac
     1741 gattctggct tgctcatgac caataaaaac ggctccatcg atgcgcacgg gcgtgatcgc
     1801 ccggcgtccg gcgacgcggc aattattgaa atcgatgccg cgaataaatg gtatggcggt
     1861 tttcaggcgc ttaccgacgt gaacttgcgt gttcagaccg gcgagcgcat tgttatctgc
     1921 gggccgtctg gttcgggcaa gtcgacgctg attcgctgta tcaatgggct ggaaaaggtg
     1981 caaaaagggc gcattgtggt cgacggcatc gatttaaccg cgggctcgcg caatatcgaa
     2041 gcggttcggc aggaagttgg catggtgttt cagcagttca acctttttcc tcacatgacc
     2101 atcttgcaaa actgcatgct tgcgccgata cgttcgcggc gtgtcagcaa acagcaggcc
     2161 gaagcgcttg ccatgcgttt tctggatcgg gttcgcattg cggaccaggc taaaaaatat
     2221 cccagccagt tatccggtgg gcaacaacag cgggtggcga tcgcccgcgc cctgtgcatg
     2281 acgcccaagg tgatgttgtt cgacgagccc acatcggcgc tcgatccgga aatggttaaa
     2341 gaggtgctcg ataccatgat tgggctggcc gatgatggca tgactatgct gtgcgtgacg
     2401 cacgagatgg gatttgcgcg aaaggtcgcc aatcgggtca tttttatgga ccagggaaaa
     2461 ataatcgagc aggctccgcc ggatcagttc ttcaacaatc cgcagcatga aaaaacgcgt
     2521 aacttcctgg gtcagatatt gaattcctag ttgggaataa aattctggct tatctgaaat
     2581 ttgcgcgaga agggaaacaa catggccggg cctcttgatg tcgtgacgtt tggcgaagcc
     2641 atgctgctgc tggttgccga tcagccgggc ccgctggagc gggtggagat gtttcacaaa
     2701 cgcacggccg gggcggaaac caatgtggcg atcggcctcg cgcgtttggg attgaaggtg
     2761 gggtgggcga gccggttggg cgccgatatg atggggcgtt atttattggc cgagatgcgc
     2821 cgcgaaggca tcgattgttc gcacgtcgtg tgcgatccgc atcagtacac ggggcttcaa
     2881 ttcaaaggcc gggtcgacga tggcagcgac cctccggttc aatatcaccg gcaagggtcg
     2941 gccgccagcc agatgcaacc cgccgatatt gatcaggcat ggctatgcag cgcaagacac
     3001 ctgcattgca ccggtgtttt cgcggcgatc tccagtaatt gttttcaggt gtctgtgaat
     3061 gcggccgcgc tgatgcgcgc tgcgggccgg acgatttcat tcgatccgaa tatcaggccg
     3121 acgttgtggt cttccgtcga ttttatgcgc cagtcgatca atcagttggc gttcgcctcc
     3181 gattgggttt tcccagggct gggggaaggc cgtattttga ccgggcagga cacacccgag
     3241 gcgattgcgc gcttttatcg cgaacacggc gcgaaactgg tggcggtcaa acttggcgct
     3301 gacggcgcct atttcgacgg gccggacggg tgcggctatg tggacagcta tcctgtaaaa
     3361 aacgtggtcg acactgttgg cgcgggagac ggctttgcgg tgggggtcat tagcgcactg
     3421 ctcgacggat gtgaactgcc tgacgccgtc aggcgcggcg catggattgg cgccagagcc
     3481 gtccaggtgc tgggcgatac cgagggcttg cccacacgcg cgcagcttga agaggctggg
     3541 ttgtaaccgg cgtcggaagc gaattcaggg tgcgggcatg cttgccagcg agcaagcctg
     3601 cagggccagt tgcgtaatgg catcccattg tctggcggcg acggccgcgc caggcgtcag
     3661 ccatgaaccc ccgacacatt ttacgttggg caggtccaga tattttttgg cgttggctgc
     3721 attgatcccg ccggtagggc aaaaaacgat atccggcaaa gggccgtaca gcgcctttaa
     3781 cagcgcgacg ccgccaattg cttctgccgg gaacagcttt tgcacggtaa agccatgatc
     3841 cgccgcgatc atcgattcgg aagccgaggc cacgccaggt aaaaacggga tgcttgcggc
     3901 gcttgccgcc ttggctatgc ccggcgtcag accggggcta accgcgaact gagcccccgc
     3961 cttgactgcc gcgtcaagct ggcgcgcgtc gcgcacagtg cccactccca ccagagcgtc
     4021 ggggacggca ctggcgatca gccggatggc gtcgagcgcc gccgctgagc gcaaagtaat
     4081 ttccaggctg cgtacgccgc ctctgaccag ggcctgggcc agggccacgg cggagtcaat
     4141 gtctttgatg acaatgacgg gcattacggg gcttttctga agcaattcaa gcgcgttcac
     4201 gatggggcgt tccttaattc gaacggtaga cggccagggc catcattgcc caggccgcaa
     4261 taaaggccaa tcctccgatg ggcgttattg cgcccagcca tttgatgcct gtcagggcca
     4321 gtatataaag gctgccgctg aaaaggagca tgccggcgaa catcaccgtg cctgcatggc
     4381 taagcagagg cgagccgaac cgcgcaccca aaacggctat cgccaataga cccaagccgt
     4441 gaataagctg gtagaacacg gcggtctgcc atacggccag caattcaggc tccagcacgc
     4501 gttttagtcc gtgcgaacca aatgcgccag cagcgacccc ggcaagcagg gtaagggcgg
     4561 cgatgatgat cagatggcga tcggtcatga gaaatagcgc ttgtatgtgt tattgcgtcg
     4621 taaatagaat gatagctgcg attatcggct tattgcctta acgtgcaagt ccgggcgggt
     4681 acgatgatat gatggattcg gcgatgctgc cggttctggc gtctgatttt taatcattcc
     4741 aaggtttgcg tttatgtcga cagttgcaac ggtatccggt ggtgctgaaa aatcgattgt
     4801 ggcatcggtg ttgtatcggc ccggtcaggc gcccgtcaac gtgcccatcg acaaattggc
     4861 gcagacagtc gacgcggaaa aacaactttt gtggataggc ctgaaagaac cgcccgtcga
     4921 gttgcttgaa cagatcgggg tgcaactgcg ctttactcca agggccctgg aggaaataag
     4981 tgaatcgcac cggcgcccga agatcattga gtatcagcaa tttacattga tcgtggcaat
     5041 tacgatcgaa gttgataata tgcgccccat ttttggcgaa actcagcttt taatcggaga
     5101 agggttcctt atcacgatac gccgaggcgc cacggcaacg catattgact tgcgccagca
     5161 tcttgaaagc tatcccgaat ttttagagcg cggaagtgac tatgtggctt ccactcttct
     5221 tgatttaatg gtggatcgct acgcgacggc gcttaagcag cttgagacgg gcgtggagaa
     5281 tatggagcag cggtttcttc taaggggatt ggccaacgcg gatgtgcgcc gtttgtatcg
     5341 gcagcgccgt gacctgttgc gcatccaggg cgcaatttct ccgatggcgg aaatctgccg
     5401 tcgcttgtcg cacgtcgaaa tgggccatat cgatgccaat tgccagggat attatggcga
     5461 ggtcgctgat cgggttgtac gcctgaatga attgatcacg agcctgcgcg aagcgttggc
     5521 gttcgccttc gaggcgggcc tgatgatagg acagtcccac caaaccgata ttacgaaaaa
     5581 gctggcggca tgggcggcta tcctggcggt tcccaccgcg gtggcgggca tttacggcat
     5641 gaatttcaaa tatatgcccg agcttggctg gaagtatggt tatgccttga tcatggcggc
     5701 gatggcactg gcctgcggcg tgctgttttg gaagtttaaa aaggcgggtt ggctgtaaag
     5761 ccggataagc ggaagacatc gatcatgggc caagttacgc atcaggtgac gaatcaggtt
     5821 cccgatctgt ctgattacaa cctgtatttg agtgacaggg cgctgctcga aggagtgcga
     5881 cgcgagggcg cgggatggca tgagcaggcc ttgattgact acggtgcgca acttggattg
     5941 cgggaaagtt tttctgcggc gcacgatgcc aatcgttata agcccgaact cgaagcgttt
     6001 gaccggcatg gcgtgcgtgt ggatcgcgtt catttccatc cggcctggcg ccagtttatg
     6061 cgcatggcct tcgcgcagcg catgcattgc agcgcctggc gcgagccttg tgtcggcgcg
     6121 cacgttgccc gggcggcgac ttatctattg cacgggcagg tcgaggccgg ttctttgtgc
     6181 ccggtcacca tgacgtccgc tgcgattccc gtgttgcaac gcgagccttt gttcgaatcg
     6241 atcaagactc tgctgttttc tgaccggtac gacgggcgcg atttgccgtt ggatggcaag
     6301 gccggcatga cgataggcat gggcatgacg gagaaacagg gcggatcgga cttgcgctcg
     6361 aataccacgc atgctgaacc cgtggcggcc gcgggtcgcg gcgccacata caggctgacg
     6421 ggccacaagt ggtttttctc gtcgccggac tccgacgcgc acttggtgct ggcccggcac
     6481 aaacagattt tttcttgctt ttatgttccg cgctggcgtg ccggaggtga caaaaacgca
     6541 gtccatattc agcgccttaa agacaaagtg ggaaatagtt cgaacgcaag cgctgaagtt
     6601 gaatttattg atgcgcaggg tgtgctggtc ggcgaagaag gccgcgggat agcgactttg
     6661 gttgaaatgg ccatgtatac gcgtctggac tgcatattgg gcagcgctgc tttgctaaga
     6721 caggcgctgg ttcaggcctt gcatcatgcg aatcgtcgtt ttgtttttgg acgtgcgctg
     6781 atcgtgcagc ctctgatgca aagcgttctg accgatcttg ctctcgagag cgaggccgcg
     6841 accgttctgg cgctgcgcct ggcgcgagcg ctggatgatc cgggcgattc gttgaaccag
     6901 gcgtataggc gggttttgac accagccgcc aagttttggg tttgcaagcg tggcattgaa
     6961 gcggtggcgg aatgcatgga agtttgggga ggcaacggct atatcgaaga agcgcccatg
     7021 gcgcgcctgt atcgggagag tcccgtgaac tcgatttggg aaggctcggg caatgtgatg
     7081 tgtctggatg tcctgcgcgc gattaatcgc catcccgaga tggcggatgt gctgcacacc
     7141 actttattgc gcgattgcgc gggcgagcct ttgttaaagg aacggatggc gcgcttgtca
     7201 gatgttctgt cggcgtccgg cccaacgctg gaggccgctg ggcgttatgt ggcgcaagaa
     7261 ctcgtgttat tgtcgcaggc cagtttatta ttgcagtgcg ccccgcaggc ggtggccgat
     7321 gcttttattc aaacgcgttt tgcggggcga ggcggacgtg ttttcggagc aggtgttcca
     7381 tcggcgctgg cgggcccgat tctggagcgc gcatggagcg aatcaagtgt tgacccagtg
     7441 cagtcgcggt aaggttttgc cgatctcctt gatcccacgg ttcgggatgt gcgcggttcg
     7501 cgcgctcctg tttgccgcgg gaggcgccat tgcggggttt ggactcggcg ccatggctgc
     7561 gccgacgcat catggtggct cggcgcacga cgcctacacc cttgccggcc gggtggtgca
     7621 tgtggccgac ggcgacacgt tcacgttgct ggtagaaggc cgtgaggagc gtattcggct
     7681 ggccagcatt gatgcgcccg agacgactca cggacgagag cggccgggac aaccgttcgg
     7741 gcaagtatcc agagacgcgc tggccagact ggtagcgggg aaaaccttga gcgtgatttg
     7801 cttcgaacgg gacaggtacg agcgcagtgt gtgcaacgtt ccgctgtcgg acggttccac
     7861 cgccaatcag aaacaggtgg ctgacggtgt tgcgtgggcc aatatggagg ggcatggaaa
     7921 atacatgcgt gatcccaaaa ttcccgagct tgaggcgcaa gcccggcatt cgcgccttgg
     7981 gttgtggcga tcgcccaatg cggtcaggcc ctgggtttgg cgctatcaat gctggaagca
     8041 gaggcaatgc taagaatttc actgcgcgcc agccggtgcg tcttgcaagg tcgaaagcga
     8101 taatgcgtac gatgatcagc tcggcggctg gctgtcgatg gctggcctgc ctgctggcgt
     8161 tgggtttgtt ggcgggttgt tcacgttcgc tcgttaatag cccgtatgcg cgaggcgatg
     8221 agaagcagaa tgttttatat accgctttcg ttcagcgctc gcccaaattt ctggaccccg
     8281 ccagctcgta ttccactgac gagacccctt ttacttattc catttacgag cccctgtatg
     8341 ggtacgatta tttacgccgg ccctatacac ttgtgccacg tgccgctgcg gccgtggccg
     8401 aaccggtgta tctggatcgg gccgggcgcc gcttgcccaa tgatgtggcg ggagatcagg
     8461 tcgatgtcag cgtgtatgac atcaaaatca aacccggcat tttgttcgcg ccgcaccccg
     8521 cttttgctcg taatgccgcg gggcagtacc gctattggcc gatggcggcc aatgatctcg
     8581 aacacgcgtt ttccatcgga gatttttccg aaacaggcac gcgcgagctg accgccagcg
     8641 actatgttta tgcgtttcga cgtttggcca gcccgcgtgt gatttccccc atctatggcg
     8701 tgatggccaa gcacatcgtt ggaatgcggg cttatggcga ccggctcaag gcgcgcgatc
     8761 aggccgtgcg cgcgcaaggc gggtctgcct ctgatcaggc ctggctggat ctgcgcgagc
     8821 aaggctttga tggggtccag gcgctggacg ctcatacttt gcgcatcaag gttattggca
     8881 agtatccgca attcaagtat tggctggcca tgacgtttac ggcgcctgtt ccctgggaag
     8941 cggaccgctt ttatcatcag cccggcatgg ccgagcataa tctctctttg aacacatggc
     9001 cggtcgggac gggtccgtac atgttgaccg agtcgattca aaaccgtcgt catgtgttgt
     9061 ccaggaaccc gaattttcgg ggcgaaccat acccatgtca tggcgaacct gccgatcagg
     9121 cggccggttt gctgaaagat tgcggcaagc ctacgccgtt tatcgatcgc attgtctttt
     9181 cactggaaaa agaaagcgtg ccgttgctgg gcaaatttct gcagggctat tacgacattc
     9241 cgcaagccga tcgcggcgac tatggcgtgg cgatgcgcgt ggctgcgggc gattcggccg
     9301 aaaaggcatc gttgtaccgc agccacggcc ttcagttgct ggcttccacc gaacctcaga
     9361 tttcttattt cggctttaac tggcttgatc cggtcgtcgg gcgcggcgac acacccgctc
     9421 agcaggaaaa aaatcgcaag cttagacagg cgatcagcat tgcgttcaat tgggagcagt
     9481 acgtttcgat tttcgagaat gaccaggcgc aggtcgccta cgggccggtc cctcccggca
     9541 ttctgggtta tcaggccgcg ccgcagggga tcgatccgta tgtctacacg atggaaaatg
     9601 ggcggcctgt gcgccggtct ttggatcagg ctaaagaatt gctgacgcag gcgggttatc
     9661 cgggcgggcg caacgctcag accggcgctc cgctaatctt gcattttgat tccgcagccg
     9721 gcatgggctc gaacgccacg tttgactgga tgcggcggca attgaaggct ctgaacatac
     9781 agctcgaaat tcgggcgacc gactacaatc gctttcagga caaaatgcgc aatggcagcg
     9841 cgcagatgtt catgtggggt tgggtggccg attaccccga tgccgagaat tttttatttt
     9901 tgttgtatgg gcctaatgcg aaggcgtcca aaggcggcga gaacgcatcc aattatcaca
     9961 accccgagtt cgatcgcctg tttgagcaga tgcgttttct tgatgatggc ccggcgaagg
    10021 agcagatcgt tcacaagatg gtggccattg tccaggccga tgcgccatgg atgttcggtt
    10081 tctatccgaa atccggaggc gcttatcaat cgtgggtgag caacgccaaa ccgactcaga
    10141 tggttcggga tactcttcag tactatcgga ttgatccggc attgcgagcc aaaaaaatcg
    10201 ctcaatggaa cgcgcccgta tggtggccgc tttggctgct ggccgttttg gcactgcttg
    10261 ggctatatcc cgcctatcgt gttatcaagc ggcgcgaagg cgccacggcg ctgggttccc
    10321 aagttactgt gcttacccat tcgaggcggc catgattcgc tatattgtgc ggcgcctgct
    10381 ttacggtgtt ctggttttga ttggcgtcaa cctgcttacc ttcgttttgt tttttgcggt
    10441 gaacacgcct gacgacatgg cgcggttatc catcggcggc cagcgtgtca gccagtccgc
    10501 catcgataaa tggaagatcg agcgtggcta cgataagccg ctgtttttca atgccgacgc
    10561 cgccggtgcg gcgaaatgga ccgacaccat tttttatgcg cgctcggcgc ctttgcttaa
    10621 gtttgacttc gggttttcgg acgatggtca cgatattggt catcaaatta cgcagcgcat
    10681 gtggccaagt ctggcgttgg ctttgcccac tttcgtactc ggattgggca tttgcgtggc
    10741 gtttgccctg ttgcttgttt ttttcaaagg cacgccgctt gattttgccg gcgtggtgat
    10801 ctgcgttgtg ctgctgtcga tatcggcgct tttttatatt attgcggggc agtggttttt
    10861 ttcaaaaatg ttgcgctggg cgccatattc gggatatatc gacggctggg gcagtctgcg
    10921 atttttgatc gtgccggtgc tggttggaat tgtgtcgggg atgggcggcc aggcgcgttt
    10981 ttacagaacg cttttcctcg aagagatcag taaagactac gtacgaacag cgcgtgccaa
    11041 agggctgtcg gaaagaattg tgttgtttcg ccatgtgctg cgcaatgcgt tgttgcccat
    11101 tttgaccggc accgtatcag ccattccctt gttgttcatg ggcagtttga tttcggaatc
    11161 atttttcggt attccaggct tgggcagcta caccatcgac gcgatcaacg ctcaggactt
    11221 ctctgttgtc cgggcgatgg tgtttctggg ctcggtcctg tatatcgtgg gattgatcct
    11281 ggccgacctt tcctatacca tcgccgatcc acgagtccgg ttcgagtaaa catatgccca
    11341 aattcgtgtt tttgtggacc gatatattta ttttttgcct gttgctggcg gtgctgttat
    11401 atgcgctcaa ggcgcggcgc agtacggcgt tgcgcgcggc ttgggccagt gtggcgcata
    11461 cgccgtcggc catgtgttca gcggtggcgc tgtcgttgtt tgtgttgata ggcgtgctgg
    11521 attctgtgca ttatcggccc gtattgccgg cagtcaattc ggccggacac agcgtgcgcg
    11581 ccatagtgcc ggtttacgcg acggcgccgc gatctttatt ggacgatgtg ctgtcgttaa
    11641 cccggcttgt cgatcccgaa acgacgtatt cagcgccgct ggcgatccgg caattcacca
    11701 aagaaagcga agtcgtcaat ggcaagccgg tgcgcgatta cccgcgcttg aaatatgcag
    11761 gcgccaattt ggcgcatgat gccgatcgcg gcgccgatat atcaaggcgt ctggtttggg
    11821 gcttgttggc cggcgtcttg ggaagcgcgg caagtgctct gacattgacg ttggcgggat
    11881 tgaaaaaggg ccgaccgttc aaacgcgccg cggccgcatg gtggcggggg gaaacgcgta
    11941 ttccgtggcg gccgatgtgg atcacgcttt caatcatcat gctggtatgc gctgtggtga
    12001 tcgcgctaag cacgaactat catgtaatgg gcaccgaccg gacaggcaac gacgtattgt
    12061 ggcaagcact gaaaagcata cgcaccgcgc tggtaattgg aacgttgacg actattgcga
    12121 tgttgccgcc cgcgctcgcg tttggcattt cagcgggcta tttcaaaggg cgggtcgatg
    12181 atgtcatcca atatatttat acaaccctga catccatccc cggggtattg ctgattgccg
    12241 cctgtgtgct catgatgcag gtctatatcg acaataatcc cggcttgttc gacaccacag
    12301 cggcacgcgc cgatttgcgg ctgttcctgc tttgcctgat attggggctg acagggtggg
    12361 ccggcctttg ccgattgctg cgcgccgaaa cgcttaaatt gcgggaactt gaatatgtcc
    12421 aggcggcgcg cgcgttcggc gtcgggcact ggcgcattat gcggcggcat ttattgccca
    12481 atgtcatgca tattgtgctg attacgctgg tgcttgaatt ttcagggttg gtgctctacg
    12541 aggccgtctt gtcttatctg ggaattggcg ttgaccccag catgaattca tttggttcca
    12601 tgatcaattc ggctcgattc gagatgtctc gcgatcccat gatctggtgg aatttgctga
    12661 ccgccttcac gtttatgctg gctctcgttc tggcggccaa catatttgcc gatgccgtgc
    12721 aagacgcctt cgatccgcgt acgcgcaagt tccgccccag ccgcgcgccg gccggattgc
    12781 gcgcgccgct ggttatcccg ggcagctccg atgcggcgac aaaggatccg ttgtgatatt
    12841 tcccgcctcc gatcagatcg acaaggcgac caacgatgga ccgtctgtat tgtccgtcga
    12901 agatttgtct gttcggattg ccagcgaatc gggcgtgatc gatgctgtca aatctctggg
    12961 aatggcaatt gcgcggggac agacctttgc gctggtcggt gaatcggggt gtgggaaaag
    13021 catgaccgcc ctggctttgt tgcgtttgct gcccgaggca gcacgcacgg cttcgggcaa
    13081 gatcgggctg gacgggcttg atttgaatcg attgcccgaa tttcgcatgc gcgccatacg
    13141 aggccgccgg atagggattg tgtttcaaga gccgtccacc agcctgaacc cggtgctgag
    13201 cgtgggccag caagtgattg aaaccctgtt tgcgcatacc gcgtatcgcg gcgccgcagc
    13261 gatcgagcgc gccacctggt ggttaaaacg cgttggaatt cccaatgcgg ccgaacgttt
    13321 gcgcgattac ccatttcaat tttcgggcgg tcagaagcaa cgcgtcatga tcgccattgc
    13381 gctggccgct gaacccgcgg tgttgattgc cgacgaacct accactgctc tggatgtgac
    13441 ggtgcaggcg caaatcctgg atttgcttgc cgacattcaa aaggaaacgg ggctggcaat
    13501 tttgcttatc acgcacgatc ttgccgtggt taaaaatgta gcggatgtcg ttgcattgat
    13561 gcgggcaggc gaaattatcg aaaccagtga cgcatcgcgc tttttttcgg cgcccaggca
    13621 cccctatgcg cgcgaacttt tggccgcgat cccgaccctg gccaaacgcg ggcattcctt
    13681 gcgccctcat ttgccggcga cgccgccgcc ggccgtcgcc atacgcacca acattaattc
    13741 cgttgcgggc ggctctcccg acgcggcttc cgtgctgatt gtcgagcatt tgagtgtgtc
    13801 ttatcagaag cgcgcgggcc ttttccatcg tcagacgggc tcgaacaagg tggtcgatga
    13861 tgtttcgttt cagttgtacc gcggcgaaac gctggctttg ctaggcgaat cggggtgcgg
    13921 caagacaacg accgcgaaag ccctgttgcg cttgctggat aagcaggcgg tggttggcgg
    13981 gcgcgcgagc cttggcggac aggatctgtt gtccgcgcat ggcgcgcggt tgaaacgttt
    14041 gcgccgaatc atgcagatcg tatttcaaga tccgtttgcg tcgctcgatc cgcgcatgct
    14101 ggtgggcgaa atacttgacg aaggcgtcgc cgcgcttcgc ccggaccatt cacgcctgga
    14161 tcgccaggcg agtgtacgca atttgatcga ccgggtggga ttgcccagaa acaccatgga
    14221 tcggtatccg cacgaatttt cgggcggcca gcgccagcgt attgcgatag cacgagccct
    14281 ggctgtcgcg cccgaagtgt tgatttgcga cgagcctact tcggctctgg atgtgtcagt
    14341 gcaagcgcaa attctggaat tgttgcgtgc cttgcaagtc gaatttaaca tcgcctactt
    14401 gttcatcacg cacaattttg gggtggttga atacctgtcg gatcgaattg cagtcatgca
    14461 tgagggcaag cttgtcgaga taggagagac agagcctgtg ttgaaagcgc cgaaacatgc
    14521 ccaaacgcaa cgcctgatcg ccgccgtgcc ccgtttgtag gccccaaccg cctataatcg
    14581 ccaaaagaga aactttccta ttgttttttt cactgatgcc cccattctag ctttatgtca
    14641 ctaaaagtct ttgatcttca atgtcagcac ggccacgtct ttgaaggctg gtttggttct
    14701 cttgacaact acgaatcgca gaaggcgcgg ggtcttatcg cgtgccccat atgcgatagc
    14761 cgccagatcg tcaagaaaat ctccgcgcct cggataaacg tcaaccatgg gcgcgcgccg
    14821 gacccgggtg cggcgcccgc gcaagg
//
LOCUS       DATCXU010000310         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1145256,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000310 DATCXU010000000
VERSION     DATCXU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145256"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VIR04_06945"
     CDS             <1..152
                     /locus_tag="VIR04_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY9460517.1"
                     /translation="TISTYDDHRMAMCFSLTAFGPVPVTILDPGCVSKTFPDYFSVYN
                     RLVQS"
     gene            149..850
                     /gene="cmk"
                     /locus_tag="VIR04_06950"
     CDS             149..850
                     /gene="cmk"
                     /locus_tag="VIR04_06950"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY9460518.1"
                     /translation="MNAPADADLSLAIPVITIDGPTASGKGTLAGCVARALGWKVLDS
                     GALYRLTALAALDQKRDTSDERIVAQVAQDLDVDFKCKRVFLDGRDVTTRIREEQVGN
                     LASRIAAYGLVRRALLERQRAFRRAPGLVADGRDMGTVVFPDAPLKIFLIADVVSRAQ
                     RRCKQLKEKGFSANLANLLKDIEARDERDRARLTAPLVAASDAVTVDSSSLSIEETVK
                     VVLGLWSRSGAQKAK"
     gene            960..>2133
                     /locus_tag="VIR04_06955"
     CDS             960..>2133
                     /locus_tag="VIR04_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY9460519.1"
                     /translation="MSSIPTSAATVGGESFSELFADSIKNQDMKSGEVISAEVVRVDH
                     NFVVVNAGLKSEALIPLEEFLNDKGELEVQPGDFVSVAIDSLENGYGDTILSRDRAKR
                     LSAWLQLEKALESGEMVSGTITGKVKGGLTVMTNGIRAFLPGSLVDLRPVKDTTPYEG
                     KTLDFKVIKLDRKRNNVVLSRRAVLEASMGEERQKLLETLCEGAIVKGVVKNITDYGA
                     FIDLGGIDGLLHITDMAWRRVRHPSEVLQVGQEVEAKVLKFDQEKSRVSLGVKQLGED
                     PWVGLARRYPQGTRLFGKVTNLTDYGSFVEVEAGIEGLVHVSEMDWTNKNVDPRKVVT
                     LGEEVEVMVLEIDEDRRRISLGMKQCRPNPWKEFSINFKRGDKVRGAIKSITDFGVF"
BASE COUNT          456 a          527 c          629 g          521 t
ORIGIN      
        1 ccacaatatc gacttacgac gatcatcgca tggcgatgtg tttttcattg accgctttcg
       61 ggcctgtgcc ggtgaccatt cttgatcccg gctgcgtcag caaaacattt cccgattatt
      121 tctctgtgta caacagattg gttcagtcat gaatgctccg gccgacgccg atttgtcatt
      181 ggcgattccc gttatcacga ttgatggacc gacggcttca ggcaagggga cacttgctgg
      241 ttgcgtagcc cgcgctttgg gctggaaagt cctggatagc ggggcgttgt atcgccttac
      301 agcccttgct gctcttgatc agaagcggga caccagcgat gaacgtattg tggctcaggt
      361 cgcgcaggat cttgatgttg acttcaagtg taaacgcgtt ttcctggacg gacgcgatgt
      421 aaccacgcgt atccgcgagg aacaagtggg taatttggca tccaggattg cggcctatgg
      481 tttggtccgg cgggcgcttt tggaacgcca gcgcgccttt cgccgcgcgc caggcctggt
      541 ggccgatggg cgcgatatgg gcacggtagt gtttcccgat gcccccctca aaatattttt
      601 aatcgccgat gtggtttcaa gagcgcaacg gcggtgtaag cagttgaagg aaaaggggtt
      661 ttctgctaat ctagccaacc tgttgaaaga tattgaagcg cgcgacgagc gtgaccgcgc
      721 gcggttgacg gcgccgctgg ttgcggcaag cgatgctgta acggtcgatt cgtcgagttt
      781 atctattgaa gaaacggtta aagtagtgct cggcctttgg tcgagatcag gcgctcaaaa
      841 ggcgaaatag gcggggtgaa ccggcctttt tgcggtttgg gattattttc actccactgg
      901 ggctggcaac atgttccggt gtgtttttaa cggccatcct ggcccatgga tttaacttaa
      961 tgtcctccat ccccacctct gccgccactg ttggcggtga aagtttctcc gaattattcg
     1021 ctgatagcat taaaaatcag gatatgaagt ccggcgaggt gatctccgca gaggtcgtgc
     1081 gtgttgatca taattttgtc gtggttaacg caggcttgaa atccgaagcg ctcattcctc
     1141 ttgaagagtt tttgaacgac aaaggcgaac tcgaagtcca gccgggcgac tttgtttcag
     1201 ttgccatcga ttcgctggaa aatggttatg gcgataccat cctgtcgcgc gaccgcgcca
     1261 aacgcctctc ggcctggttg cagcttgaaa aggcgctgga gtccggtgaa atggtttccg
     1321 gcaccattac gggcaaggtc aagggcggac tgaccgtcat gacgaacggc attcgcgctt
     1381 tcttgcccgg atcgctggtc gatctgcgcc ctgttaaaga caccacgccg tacgaaggga
     1441 agacactgga cttcaaggtg atcaagcttg atcgcaagcg caataacgtt gtgctttcgc
     1501 gtcgcgcggt cctggaagcc agcatgggcg aagaacgcca gaagctgctt gaaacattgt
     1561 gcgagggcgc catcgtcaag ggcgtggtca aaaatattac cgattacggc gcctttatcg
     1621 atctgggcgg cattgacggt ttgctgcata tcaccgacat ggcgtggcgc cgcgttcgcc
     1681 acccgtccga agtcctgcaa gtgggtcagg aagtcgaggc caaagtgctt aagttcgatc
     1741 aggagaaaag ccgtgtttct cttggcgtca agcaattggg cgaagatccc tgggtcggcc
     1801 tggcgcgtcg ctacccgcag ggcacgcgtt tgttcggcaa ggttacgaac ttgaccgact
     1861 atggctcctt tgtggaagtt gaagcgggca tcgaaggttt ggtgcatgtg tcggaaatgg
     1921 attggaccaa taaaaacgtt gatccgcgca aagtcgtgac gcttggcgaa gaagtcgaag
     1981 tcatggttct tgaaatagac gaagatcgcc gccgtatttc gcttggcatg aaacaatgcc
     2041 gtccgaatcc gtggaaagag ttctccatta atttcaaacg cggcgacaag gtccgcggcg
     2101 cgatcaagtc aattaccgat ttcggcgtgt ttg
//
LOCUS       DATCXU010000311         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_284496,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000311 DATCXU010000000
VERSION     DATCXU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284496"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="rseP"
                     /locus_tag="VIR04_06960"
     CDS             <1..605
                     /gene="rseP"
                     /locus_tag="VIR04_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY9460520.1"
                     /translation="GSLKNPTADAMVKEIQAHAGQPVALTVLRNGAALSLNVVPALET
                     TPNGVKVGRIGVLLGAEFPMVTVRYGIIDSVFRAVSRTADTAWFSLKMMGRMVTGGVS
                     LRNISGPVTIADYAGQTARIGIAAYIGFLALISVSIGVLNLLPIPMLDGGHLMYYILE
                     VARGKPVPEKWRAAGQRIGLGVLAALMGLAFFNDFYRLFG"
     gene            657..3011
                     /gene="bamA"
                     /locus_tag="VIR04_06965"
     CDS             657..3011
                     /gene="bamA"
                     /locus_tag="VIR04_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY9460521.1"
                     /translation="MSLRWKFPIIKARHISAVWLAALLLPGLANAFSTFVVKDIRVNG
                     IQRVDAGTIFTYLPVKVGQRFTPAEAAEAIQRLYGSGFFSDVKIDAENNVLVVTVVER
                     PTIASVSFNGMHDLDAKAITKSLLAVGFGQGRVFDRSMLERAEFEIKQQYLSKGKYGV
                     DISSIITPLPRNRVGISFDIFEGDRAKIKQIKIIGNHAFSQGTLLDQLELTTSGVMTW
                     YTGTDKYSREKLEGDVERLRSYYLDRGYLEYSSEPAQVSISPDRQGIFITLTVHEGKP
                     YTVHGVKLAGELLGLKDKISPLVTVKDGEVFSAAKTNASAKAITDYLGSLGYAFANVN
                     PNPVLDREKHQADLTFYVDPGRRVYVRRIQIGGNTRTRDEVVRREIRQQEAAWYDASS
                     IKSSRDRIDRLGYFSDVNVHTTPVAGSPDQVDVAVDVKEKPTGLVNLGVGYGSTDKLI
                     LSAGISQDNVFGSGDTLSLQANTSAINRAVVLSHTNPYWTKDGISKTTSLYYQRTTPY
                     NVSSATGSNDNYQVTAIGAGLNFGVPISEFDRVFTGVRFERNSINLGSTPPQAYTDFV
                     NEYGRTTNAAIFNLGWSKDTRDSAIAPTKGSYTRVSGDVSTMNLRYYMLSGQQQYYLP
                     LGRSYTLAFNGLADWGKSYGGKTFPIIKDVYAGGIGSVRGYEGASLGPRDVATGNYLG
                     GSRRVAGSVQLYLPFPGATRDRTLRWFLFTDAGRVSNGSGLGCSAGFNNSSVEDPCGW
                     RYSAGVGLSWQSPLGPLQLSFGRALNAKAGDDKQAFQFQIGTGF"
     gene            3074..3634
                     /locus_tag="VIR04_06970"
     CDS             3074..3634
                     /locus_tag="VIR04_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY9460522.1"
                     /translation="MKSQMVLKLSALSRSICRGNRALVLATVLGAMVVVAAPAQVMAQ
                     GTKIGFVSTEKILRDSKPAKAAQAKIEAEFKGRDANLQRLANNLRSQAQQLDKDAPVL
                     SEAERVKRQRKLADLDADLQRKRREFQEDFNRRRNEEFSAIVEKANASIKKIANDQHY
                     DLIIQDAVTVSPKIDITDQVMRALGG"
BASE COUNT          830 a         1008 c         1077 g          881 t
ORIGIN      
        1 tcgggtcgtt gaaaaatcca acggccgacg ccatggtgaa ggagatccag gcgcacgcag
       61 gccagcccgt cgccctgaca gtgttgcgaa atggggccgc tctgtcgctt aatgtggtcc
      121 cagcgctcga aaccacgcct aatggtgtga aagtggggcg tatcggcgta ttattaggcg
      181 ctgaattccc gatggtcact gtccgttacg gaataatcga cagcgtcttc agggctgtgt
      241 cgcgtacagc cgacactgcc tggttttcat taaaaatgat gggccgcatg gtaacggggg
      301 gcgtttcact gcgcaatatc agcggtcccg tgacaattgc cgattatgcg ggtcaaacgg
      361 cccgcattgg tattgcggcg tatataggct ttttggccct gatcagtgtt agtatcggcg
      421 tgttgaattt gctacccatt ccgatgctcg atggcggcca tttgatgtac tacattttag
      481 aagttgcccg cggtaaaccg gtgcccgaaa aatggcgcgc cgcgggtcaa cgtattgggt
      541 tgggcgtgct ggcggcattg atggggttag cattttttaa cgatttttat cgcctttttg
      601 gttagagacg cttctgtctt acaattttcc atcacctcga gcaaggactc accaggatgt
      661 cgttacgctg gaaattccct attattaaag ccaggcacat atcggctgtt tggctggcgg
      721 ctttgctgct tcccggcctt gccaatgctt tttccacctt cgtggtgaaa gacatacgcg
      781 tcaacggcat ccagcgtgtc gatgccggaa caatattcac ctacctgccg gtaaaggttg
      841 gtcaacgatt tacgccagcc gaagccgccg aggctattca gcgtctgtat gggtcggggt
      901 ttttcagcga cgtgaaaatt gatgccgaaa ataacgttct ggtggtcacc gttgtagagc
      961 ggcccaccat cgcgtcggtc agttttaacg gaatgcacga tctcgacgcc aaggccatta
     1021 ccaagtcgct gcttgcggtg gggtttggtc agggccgcgt attcgatcgc tcgatgcttg
     1081 agcgcgccga atttgaaatc aagcagcaat atctttccaa aggcaaatac ggggtcgata
     1141 tcagttcgat catcacgccc ctgccgcgca accgggtagg gatcagcttc gatattttcg
     1201 agggcgaccg ggccaaaatc aagcaaatca agattattgg caatcacgct ttttctcaag
     1261 gcacgctgct tgatcagctt gaactgacca catccggcgt catgacgtgg tacaccggca
     1321 ccgacaagta ttctcgcgaa aaactcgaag gcgacgttga gcgcttgcgt tcctattacc
     1381 tggatcgggg ctatcttgaa tattcttccg agccggcaca ggtgtcgatc tcgcctgacc
     1441 ggcaaggaat attcattacg cttaccgtgc acgaaggcaa gccttacacc gtgcatggcg
     1501 tcaagttggc cggggaattg cttggcttga aggacaaaat ttcaccgctt gtcacagtaa
     1561 aggacggcga ggtgttttcg gcggcaaaga ccaatgcaag cgccaaagcc attacagatt
     1621 acctgggcag tcttggctat gccttcgcca atgtcaaccc gaacccggtg ctcgaccggg
     1681 aaaaacacca ggccgatctg accttttacg tggatccggg gcgacgcgta tatgtgcggc
     1741 gaattcagat tggcggcaac acccgcacgc gcgacgaagt cgttcgccgc gaaattcgcc
     1801 agcaggaagc ggcctggtac gatgcgtcca gcatcaagtc atcgcgtgat cggatcgacc
     1861 gtcttggcta cttcagcgat gtcaatgtgc atacaacacc ggttgcgggt tcgcccgatc
     1921 aggtggatgt tgccgtcgat gtaaaagaaa agccgacagg tctggtgaac ttgggcgtag
     1981 gttacggctc gaccgacaaa ctgattttgt cggcgggcat tagtcaggac aatgtgtttg
     2041 gcagcggtga tacgctgtct ttgcaggcga acaccagcgc aatcaacaga gcggtggtgt
     2101 tgtcccacac caatccatat tggaccaagg acggcattag caaaaccacg tcgctctact
     2161 accagcgtac gactccctac aacgtgtcca gcgcgacagg cagcaacgac aattaccagg
     2221 taaccgcgat tggcgccggc ttgaacttcg gcgtgcctat ttccgaattt gatcgcgtct
     2281 ttactggcgt gcggttcgaa cgcaacagca taaatctggg tagtacgccg cctcaggctt
     2341 acaccgattt cgtcaacgag tacggccgta cgaccaacgc cgccatcttc aatctgggat
     2401 ggtccaagga tacgcgcgat agcgccatag cccccaccaa aggcagctac acacgcgttt
     2461 caggcgatgt gtccaccatg aatttgcgtt actacatgtt aagcggtcaa cagcagtact
     2521 atttgccgct gggtcgcagc tatacgctcg ctttcaacgg cctggccgat tggggtaaat
     2581 cgtacggcgg caagaccttc ccgattatca aagacgtata cgccggcggc attggttcgg
     2641 ttcgcggcta cgaaggtgca tcgctggggc cacgcgacgt agcaacggga aattatctgg
     2701 gcggttcgcg gcgtgttgcg ggcagtgttc agttgtattt gccgttcccc ggcgcaacgc
     2761 gtgatcgcac cttgcgctgg ttcctgttca ccgatgcggg tcgggtttcc aacggtagcg
     2821 gcctgggctg ttcggcgggc tttaacaata gctctgtgga agatccgtgc ggctggcgtt
     2881 attctgcagg tgttggcttg tcgtggcagt cgcctctggg cccactgcaa ctgtcatttg
     2941 gccgtgcact caacgccaag gcgggcgacg acaaacaggc gttccagttc cagatcggca
     3001 ctggtttttg atgtggctta tttaatggca caataacctt ttttatctct gctctgtgca
     3061 aggtagattt ttcatgaagt ctcaaatggt attaaaactt tcggcgctaa gccgtagtat
     3121 ttgtcgcggt aatcgggctt tggtgctcgc aacagtgctg ggcgcgatgg tggtcgtggc
     3181 ggcgccggcc caggtaatgg cgcagggaac gaagatcggt ttcgtgagca ctgaaaagat
     3241 tttgcgcgac tcgaaacccg ctaaagccgc ccaggccaaa atcgaagccg agttcaaggg
     3301 ccgtgatgcc aatctgcaac gtttggccaa taatttgcgc agccaggctc agcaactcga
     3361 taaagacgct cccgtgctgt ccgaagccga acgcgtcaag cgtcagcgca agctggccga
     3421 tctcgatgcc gacctccaac gcaagcggcg cgagttccag gaagacttta atcgccggcg
     3481 caatgaagag ttctcggcta ttgtggaaaa ggccaacgct tccatcaaaa agatcgcaaa
     3541 tgaccagcat tatgacttga tcattcagga tgccgtcact gtcagcccaa agatcgatat
     3601 caccgatcag gtgatgcgag cactgggcgg ttaaggttct atgccggtat tactggcgcc
     3661 agaccaggcg cctaccttgc aggaactatt ggctgacgcg aacaccatcg gtttgggcta
     3721 cgagatcgtc gacttgccgg gcaagtctgc gccggctatt caggggctta gctcgctgct
     3781 tgcggcgggc gccacc
//
LOCUS       DATCXU010000312         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_324890,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000312 DATCXU010000000
VERSION     DATCXU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324890"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VIR04_06975"
     CDS             <1..389
                     /locus_tag="VIR04_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460523.1"
                     /translation="NVGAYSAEIMRAGFDSIEKQQLEAAVSLGLGRWQIYWHVILKQA
                     MERMYPALTSQFVLLMLASSICSQISAEELTAVANFIQSDTYRPFETYIVVGGLYIFL
                     SVLLRVAFWIFGLVLFPRRRNVLALT"
     gene            402..1058
                     /locus_tag="VIR04_06980"
     CDS             402..1058
                     /locus_tag="VIR04_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460524.1"
                     /translation="MGQSFAVGYLVFLLKGALWTVGLSTIALCCGGVLALIVALCRIS
                     TQRWLRLITALYIQIIQGTPVLVIIFLTYFGLPSIGWKVSPLMAASVSLSVYVSAYLG
                     ESWRGSIQSIPKAQWEAAECLALSRTQRMLKVILPQTIRIATPPTVGFMVQIIKNTSL
                     ASVVGLVELVRAGQLVNNTTFKPFLIYLIIAGLYFAMCYPISIFSRKLEARAGEQSYA
                     "
     gene            1051..1806
                     /locus_tag="VIR04_06985"
     CDS             1051..1806
                     /locus_tag="VIR04_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9460525.1"
                     /translation="MLNVIDDRPADVIVSLKEVHKKFGANEVLCGVNLDVVRGEVVAI
                     IGQSGSGKSTALRCIDLLEHINSGAITVCGHELQNSNVDLKALRLDVGIVFQSYNLFP
                     HLTIEGNVMLALTSVKKMPKKEARDLAHRVLGQVGLLEKVKQYPDQLSGGQQQRAAIA
                     RSLAMEPKVMLFDEVTSALDPQLTGEVLRVIEDLANKGMTMIMVTHEMNFARKIADTI
                     VFMNQGRVWEKGTADMLNHPRTPELREFLQNEL"
     gene            1831..2631
                     /locus_tag="VIR04_06990"
     CDS             1831..2631
                     /locus_tag="VIR04_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460526.1"
                     /translation="MKRRTFAASLLCMAGLLGASISVANADVLDNIMKTKTIRISTDL
                     GIPPAGMLSPTMEPMGADVEVGKLLAHDWGVKYELIQTTGATRIPNLQTDKADIVVST
                     LSVTPQRAQVIDFSHRYAVLQSIVAGYSDLPVKSLETLKGRTVSVTRGTTQDTELTPQ
                     ASKYGFTVARYDDDATLVTASVSGQAKIIATSQSLVAQIDKKIGKNNFKPLFVLRNFD
                     LAIGVKKGETRLLAKVNEWVDANVKNGKLNAIYKKYYGVELPENMRGK"
     gene            2663..3106
                     /gene="rpiB"
                     /locus_tag="VIR04_06995"
     CDS             2663..3106
                     /gene="rpiB"
                     /locus_tag="VIR04_06995"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY9460527.1"
                     /translation="MRIVIGSDHAGWPLKKSVVDHIKALGHEVKDVGSFNDQPVDFPD
                     IAKALTNEIKSGRSDRGIMVCGTGVGASIAANKVKGIRAAVCHDVHSAHQCVEHDDVN
                     VMCMGAQIVGPWLANDLVAAYLGAKFSTDEDCRRRVDMLHDMDQA"
BASE COUNT          771 a          801 c          911 g          774 t
ORIGIN      
        1 ttaatgtcgg cgcctattcg gcggaaatca tgcgggcagg attcgattcg atcgagaagc
       61 agcaactgga agccgctgtc agcctggggc tgggtcgctg gcaaatatat tggcacgtca
      121 tcttgaagca ggcaatggaa agaatgtatc cggccctgac cagccaattc gtgttgttaa
      181 tgctggcttc ctcgatttgc tcccagattt cggccgaaga gttaaccgcg gtcgccaatt
      241 ttattcaatc ggatacctac cgcccgtttg aaacttacat tgtggtgggc ggcctgtata
      301 ttttcttgtc tgtgctttta agagtggcgt tctggatttt tggtctggtg cttttcccac
      361 gtcgcagaaa tgttcttgcg ttgacttagc ccggaggcaa catgggtcaa agctttgcgg
      421 ttggatatct ggtgtttttg ctaaagggcg cgttgtggac cgttggactg tccacgatcg
      481 ctttatgctg cggcggcgtg ctggcgctga tcgttgccct gtgccggatc tcgacccaaa
      541 gatggttaag actgatcacc gccttataca tacaaatcat ccagggcact ccggttttgg
      601 tcattatttt cctgacctat ttcggcctgc cgtcgatagg ctggaaagtg tctccgctga
      661 tggcggcatc cgtgtcgctg tccgtatacg tttcggccta tctgggtgaa agctggcgcg
      721 gctcgataca gtctatccca aaggcccagt gggaagccgc cgaatgcctt gccttgtccc
      781 gcacgcaaag aatgctgaaa gtgattctgc ctcaaacaat ccgaatagcc acgcctccga
      841 ccgttggatt tatggttcag attatcaaaa acacctcgct tgcctcggtg gtcgggctgg
      901 tcgaattggt acgggcgggg cagcttgtga ataacacgac cttcaagccg ttcttgatct
      961 acctgattat cgcgggcctt tactttgcca tgtgttatcc catttcaata tttagccgca
     1021 agctcgaggc cagggctgga gaacagtctt atgcttaatg tcatcgatga ccggcccgcg
     1081 gatgtcattg tcagcttgaa agaggtacat aaaaaatttg gcgccaacga ggttttgtgc
     1141 ggcgtaaatc tggacgtcgt tcgtggcgaa gtggttgcca tcattgggca aagcggctcg
     1201 ggcaaaagca cggcgcttcg ttgcatcgat ctgctggagc acatcaattc gggcgccatt
     1261 acggtgtgcg gccacgagct ccaaaattcg aacgtcgacc tcaaggcatt aaggctggat
     1321 gtgggcattg tttttcaaag ctataacctt ttcccgcatt tgacgatcga aggcaatgtc
     1381 atgctcgccc ttacgtcggt caaaaaaatg cccaaaaagg aagcccgcga tttggcgcat
     1441 cgggtccttg ggcaggtggg gttattggaa aaggtaaagc aatatcccga tcagttgtcg
     1501 ggcgggcagc aacaacgcgc cgccattgcc agatcgttgg cgatggaacc aaaagtcatg
     1561 ttgtttgacg aggtaacgtc ggcgctggat ccccaattga cgggagaggt gctgcgcgtt
     1621 attgaagacc ttgcgaacaa gggaatgacc atgatcatgg tgacccacga gatgaatttt
     1681 gcacggaaaa ttgcagacac tattgtcttc atgaaccagg ggagggtatg ggaaaaagga
     1741 acggcggaca tgctgaatca tccgcgcacg ccagagttga gagaattttt gcagaatgaa
     1801 ctttagtcat taaacaaccg gagacacacg atgaaaagaa gaacctttgc ggcttcacta
     1861 ttatgcatgg cgggtttgct tggcgcgtcc atctcggtgg cgaatgccga tgtgctggac
     1921 aacattatga aaaccaagac gatccgtatt tcaacggacc ttggaattcc gccggcgggc
     1981 atgttaagcc ccaccatgga acccatgggg gccgatgtcg aagtcggcaa gctgctggct
     2041 catgattggg gagtcaaata cgagctgatc cagacgaccg gcgcgacccg cattcccaat
     2101 ctgcagaccg acaaggccga tattgttgta tcgaccttgt ccgtgacccc gcaacgcgcg
     2161 caagtcatcg atttttcgca tcggtatgcg gtcttgcagt ctatcgtcgc gggttatagc
     2221 gatctgcctg ttaaaagcct ggaaacgtta aaaggcagga cggtttctgt cacacgcgga
     2281 actacgcaag acaccgaact gactccgcag gcgtccaaat acggattcac cgtcgcgcga
     2341 tatgatgacg acgcaacgct ggtgacggct tcggtcagcg gtcaggccaa aatcatcgcc
     2401 acgtcgcagt cgctggttgc tcaaatcgat aaaaagattg gcaaaaacaa tttcaagcca
     2461 ttattcgttt tgcgcaattt tgatctggcc attggtgtca aaaaaggcga aaccaggttg
     2521 ttggcaaagg tcaatgaatg ggtcgatgcc aatgtcaaga atggtaaatt aaacgcaatc
     2581 tacaaaaagt attacggtgt cgagctgccg gaaaatatgc gtggaaaata gcttatcgta
     2641 ttaaaaattc aggaaatgaa ctatgcgtat tgttatcggt tccgatcacg ccggatggcc
     2701 tttaaaaaaa tcggtcgtgg atcacattaa agccctcggc cacgaagtaa aagacgtggg
     2761 cagttttaac gaccaaccgg ttgatttccc ggacatcgcc aaagcgttga cgaacgaaat
     2821 caagtcgggc cgatccgatc gcggcatcat ggtttgcggc acaggggtgg gggcgtccat
     2881 cgcagccaac aaggtgaaag gtatccgcgc ggcggtttgt cacgacgttc attccgcgca
     2941 tcaatgtgtc gagcacgacg atgtcaacgt catgtgcatg ggcgcgcaaa ttgtcggtcc
     3001 ctggctggcc aacgacttgg tggcggcgta cctgggcgcc aaattttcca ccgacgagga
     3061 ttgccggcgc cgggtcgaca tgctgcacga catggatcag gcctgatgtg aagaagacaa
     3121 tggcggctaa agcgcccaaa atcttcatcg ccgggatcta cgctatggat ctggttttca
     3181 ccgggcgccg catgcccgcg cccggtgaaa ccgttccggc atccggtttt atgcgcggac
     3241 acggcggcaa agggtcg
//
LOCUS       DATCXU010000313         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_166403,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000313 DATCXU010000000
VERSION     DATCXU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166403"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VIR04_07000"
     CDS             <1..449
                     /locus_tag="VIR04_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY9460528.1"
                     /translation="SAQAAKEIKVLIEESIGKVNAGAKQAKQAGQVMQEVVGSVDGVM
                     TIMGEISSASREQSDGIDQVNQAVVEMDGVVQQNAALVQEAAAAAGSLQDQTKRLTEA
                     VAVFRLTANEVIDVATQQLKQTESKSGAAANALPPDSALRALAHYS"
     gene            459..1298
                     /locus_tag="VIR04_07005"
     CDS             459..1298
                     /locus_tag="VIR04_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY9460529.1"
                     /translation="MSLPASSLFTLPVLPEFGQGDFERAAHLLQRRTGIVLGDHKREM
                     ACRTLGIRTRKLGLSNVADYLIHLDQNAQSPEWDAFVNAFTINHTAFFRERHHFKALA
                     ELVQERNAPLSVWCSAASTGEEPYTIAMTLHEAGLPLPLTPPVWATDIDTKAIADARQ
                     GIYSLERVKPVPEDYLKKYFYRGRDRRAGMVRVKPNIRNSVRFETFNLLSDSWPLGDK
                     FDVIFCRNTMIYFDKPTQTRILKRFASMLRPQGLLFAGHSENFTYLTDAFHLRGKTIY
                     VRS"
     gene            1350..2402
                     /locus_tag="VIR04_07010"
     CDS             1350..2402
                     /locus_tag="VIR04_07010"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY9460530.1"
                     /translation="MQKIRVLCVDDSALVRGLMTEILDSQDDMEVVATAADPLIAREL
                     IKQHNPDVLTLDVEMPRMDGLDFLERLMRLRPMPVIMVSSLTERNSEVTLRALELGAV
                     DFVTKPKLGLRDGLVEYTDLIADKIRAAAHSRPRRSAQPGAAPGRRLENPFSTTEKLV
                     LIGASTGGTEAIRQVLEPMPANSPAILITQHMPAGFTRSFVQRLDHLCAVQVHEAEHG
                     QRVLPGHVYLAPGGVAHMKLARSGANYVVNLEYSDPVNRHRPSVDVLFHSAALQAGKN
                     AVGVILTGMGKDGAQGLLAMHEAGAITFAQDEASCVVFGMPREALLIGAATDAVPLSG
                     ISERILASAGSYGHRV"
     gene            2448..2840
                     /gene="cheY"
                     /locus_tag="VIR04_07015"
     CDS             2448..2840
                     /gene="cheY"
                     /locus_tag="VIR04_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEY9460531.1"
                     /translation="MVQKTIKILVVDDFPTMRRIIKNLLKDLGFENVDEAEDGVQGLE
                     KLRSGGLFELVVSDWNMPNMDGLTMLKEIRADAALAKLPVLMVTAEAKRENIIAAAKA
                     GASGYVVKPFTAAILEEKLNKIFEKIGA"
     gene            2852..3493
                     /gene="cheZ"
                     /locus_tag="VIR04_07020"
     CDS             2852..3493
                     /gene="cheZ"
                     /locus_tag="VIR04_07020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEY9460532.1"
                     /translation="MDVQNDLETNRTLEQPVDLIHRVAQLTRMLRESMRELGLDQAIK
                     DAAQAIPDARDRLHYVAKMTEQAANRVLNAAEAMQPLQESMQRDARALEKRWQAWFDH
                     PVELEHARGLVEDTRGLLRAIPDKTQLSQNSLMEIIMAQDFQDLTGQVIMKMLVVVTA
                     IEKELVQVLIDNVPQEVRDDAYSLLNGPQTSPLGKDVVTGQDQVDDLLANLGF"
     gene            3503..5020
                     /locus_tag="VIR04_07025"
     CDS             3503..5020
                     /locus_tag="VIR04_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY9460533.1"
                     /translation="MKHRASRTIGIFRLLRHVDRGRATLAERAWTLSPLAWPLSRLLK
                     NLRSSVIGMRQFSMDIALNTARLLSHTQTCTELARQQAVETEGLASSGSQIEGLSEQT
                     AMRAKDIASTFGAQLAVAQQTLAQLNELHERVTRVATQMEVFSGVVAQLSQRAQSVGD
                     TSRLIKDIALQTHLLALNAGVEAARAGEAGKGFAVVASEVGKLAERVNSATGEIVRAT
                     GEILELVADTREKTDHIHLDMTTSDTVVTQFASSFDTFVKDFGRMESQMAEVVQNVSQ
                     VNETNHSMSRTISSIAALSSQVQNRMATMNDEVNSVRDKSESLQELLATLRAGNTPFD
                     WLSGMLDSLRDACRRLLLQARQQGADILDVRYQRIPNSNPARFHTSYDTLIDQQLTRI
                     LDYVLDQLPAGYYALLVDKNGYCPTHNTRYSRASTGELAHDTLYVRNKRIFDDRVGKR
                     AAGNTSEILCQTYMRDTGEIISDLSIPLDIDRMHWGAVRIGIDYSRFEEILQANA"
     gene            5195..>5340
                     /locus_tag="VIR04_07030"
     CDS             5195..>5340
                     /locus_tag="VIR04_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY9460534.1"
                     /translation="MAEESDLEKTEAATPQRLEKAREEGQVARSRELNTFLLLAAGVG
                     GLWFG"
BASE COUNT         1202 a         1393 c         1578 g         1167 t
ORIGIN      
        1 gcagcgcgca agccgctaag gaaatcaagg tgctgatcga agagtcgata ggcaaagtca
       61 acgccggggc caagcaggcc aaacaagccg gccaggtcat gcaagaggtt gtaggctcgg
      121 ttgacggagt catgacgatc atgggcgaaa tttcctcggc ctcgcgcgag cagtccgacg
      181 ggatcgatca agtcaatcag gcggtggtcg agatggacgg cgttgtgcag cagaatgcgg
      241 ctctggtgca agaggctgcg gccgcggcgg gttccttgca ggaccaaaca aagcgcctga
      301 ccgaagctgt ggcggtattc agactgactg cgaacgaggt cattgatgtg gcgacccagc
      361 aattgaagca gacagaatcg aaatccggag ccgcggccaa tgctttgccg cccgattcgg
      421 cgctacgcgc gctggcgcat tattcttagg atttatcgat gtccttgccc gcttcgtctc
      481 tgtttacttt gcccgtttta cccgaattcg ggcaaggtga ttttgagcgt gcggcgcatt
      541 tgctgcaacg ccggacgggc attgtgctgg gcgaccacaa gcgcgagatg gcgtgccgca
      601 ctttgggtat ccgaaccagg aaattgggtc tgtccaatgt ggccgattat ctgattcatc
      661 tcgatcagaa tgcacaatcg cccgagtggg acgcgtttgt aaacgcattc acgatcaacc
      721 acacggcgtt ttttcgcgaa cgccaccact tcaaggcgct ggcagagctg gttcaggagc
      781 ggaatgcgcc actatcggtc tggtgcagcg ccgcgtcgac gggcgaggag ccctacacga
      841 ttgccatgac gctgcacgag gccggcttgc ctcttccgtt aacccccccg gtttgggcaa
      901 ccgacattga tacgaaggcc attgccgatg cccggcaggg catttattcg ctggagcgtg
      961 tcaaacctgt gccggaagac tacctgaaaa aatattttta tcgaggccgg gacaggcggg
     1021 cgggcatggt tcgggtcaag ccgaacattc gcaattcggt tcgattcgaa acgtttaatt
     1081 tattgtccga cagttggcct cttggcgaca aattcgacgt gattttttgc cgcaatacga
     1141 tgatttattt tgataaaccc acgcagactc gaattttgaa gcggtttgcc tcgatgctta
     1201 ggccccaggg gctattgttt gccgggcatt ccgaaaactt tacttatctg accgatgcct
     1261 ttcacttgcg ggggaaaacc atctatgtgc gttcctgaca atggcgcgac ccggttctat
     1321 ctgcgcctct ttttaacaga ggtctgacat tgcaaaaaat ccgtgttttg tgcgtggatg
     1381 actcggcatt ggtgcgcggc ctcatgactg aaattcttga cagtcaggac gacatggagg
     1441 tggtggccac tgccgccgat cccctgatag cgcgagagct catcaaacag cacaatcccg
     1501 acgtgctgac gcttgacgtt gagatgccgc gcatggacgg gctggatttt ctcgaaagat
     1561 tgatgcgctt gcggccaatg ccggtgatca tggtgtcctc attgactgag cgcaattccg
     1621 aggtaacttt gcgggcgctt gaactgggcg cggtcgactt tgtgaccaag ccaaaattgg
     1681 gcctgcgcga tggcttggtc gagtacaccg accttattgc cgataaaatc cgcgcggccg
     1741 cgcattcgcg tccgcgccgt tcggcgcaac ccggcgctgc accgggaagg cgcctggaaa
     1801 atccattttc gacgaccgaa aaactggtgc ttattggcgc ttcgaccggc ggcacggagg
     1861 cgattcggca ggtcctcgaa cctatgcctg caaacagtcc ggccatactt atcactcagc
     1921 atatgccggc cggcttcacg cgctcgttcg tgcagcgtct ggatcatttg tgcgctgtcc
     1981 aggtgcacga agcggagcac ggacagcgag tattgcccgg ccatgtttac ctggcgccgg
     2041 ggggcgtggc gcatatgaag ctggccaggt cgggcgccaa ttatgtagtc aatctggagt
     2101 acagcgatcc ggtcaaccgg catcgcccat ctgttgatgt attgtttcat tcggccgcgc
     2161 tgcaggcggg caaaaatgcc gtcggcgtga ttttgacggg catgggcaag gatggcgcgc
     2221 agggcttgct tgccatgcac gaggcggggg cgatcacttt tgctcaggac gaggccagtt
     2281 gcgtcgtctt cggcatgccg cgcgaagccc tgcttatagg cgccgcgacg gatgcggtgc
     2341 ctctgtcggg gataagcgaa cgcattttgg caagtgccgg ttcttatgga catcgggtat
     2401 agcatcgctt cggttagcgg gctatttatt attcatggag agtataagtg gtgcaaaaaa
     2461 ccataaaaat tctggtggtt gacgactttc ccacaatgcg tcgaattata aaaaatttgc
     2521 tcaaagattt ggggttcgaa aacgttgacg aggccgaaga cggggtgcag gggctcgaaa
     2581 agctgcgcag cggcggctta ttcgagctgg tagtgtccga ctggaatatg ccgaacatgg
     2641 atgggctaac catgttgaaa gaaatcaggg ccgacgccgc gctggcgaag ctgcctgttt
     2701 tgatggtcac cgccgaagcc aaaagggaaa atattatcgc cgccgccaaa gcgggcgcca
     2761 gcggctatgt cgtcaagcct ttcaccgccg ccatcctgga agaaaaactg aacaaaattt
     2821 ttgaaaaaat aggcgcataa ggaataagaa gatggacgtt caaaatgatc ttgaaacgaa
     2881 cagaactctg gaacagcctg tcgatttgat tcatagagtc gcccaactga cgcgcatgtt
     2941 gcgcgaaagc atgcgcgaat tgggcctgga tcaggcgatc aaggatgcgg ctcaggctat
     3001 tcccgacgcg cgcgatcgtt tgcattatgt ggccaagatg accgaacagg ctgccaatcg
     3061 cgtattgaac gcggcggaag cgatgcagcc gttgcaggaa agcatgcaac gcgacgccag
     3121 agcattggaa aaacgctggc aagcctggtt tgatcatccg gtggagctcg aacacgcgcg
     3181 cggcctcgtt gaagacacgc gcggtttgtt gcgagccatt cctgacaaga cgcaactttc
     3241 gcaaaacagc ctcatggaaa tcatcatggc gcaggatttt caggacttga ccggccaggt
     3301 catcatgaaa atgctggtcg ttgttacggc gatagaaaag gaattggtgc aggtgctgat
     3361 tgacaatgtg ccgcaagaag tgcgtgacga tgcgtattcc ctgctgaatg gaccgcagac
     3421 aagtcctctg ggaaaagacg tggtgaccgg ccaggatcag gtggacgatt tactggccaa
     3481 tcttggtttt taaggggatg aaatgaaaca cagggcttct cgcacgatcg gtatattccg
     3541 gctacttcgc catgtcgacc gggggcgggc tactctggcc gagcgagcct ggacgttaag
     3601 cccgctggcc tggcccttgt cccggctttt aaagaatttg cgcagcagcg taatcggaat
     3661 gcgtcagttc agcatggata tcgctttgaa tacggcgcgc ctgctatcgc atacgcaaac
     3721 gtgcactgaa ttggcgcgcc agcaggccgt cgagacagaa ggcctggcct ccagcgggag
     3781 ccagatcgag gggctttccg aacaaacggc catgcgcgcc aaggacatcg cttcaacttt
     3841 cggcgcccag ttggcggtgg cccagcaaac cctggcccaa cttaatgaat tgcacgaacg
     3901 cgtcacccgt gtcgcgacgc agatggaggt tttttcgggt gttgtggccc aattaagtca
     3961 acgcgcccag tcggtcggag acaccagccg cctgattaaa gacattgctt tgcaaacaca
     4021 cttgctggct ttgaatgcgg gtgtcgaagc cgcacgcgcc ggggaagcag gtaagggttt
     4081 cgctgtggtt gccagcgaag tcggcaagct ggccgaacgg gtcaactcgg cgactggcga
     4141 gattgtccga gctaccggcg aaatcctcga gcttgtggcc gacacacgcg aaaaaacgga
     4201 tcacattcat ttggacatga cgacatcaga caccgtagtc acgcaatttg cgagcagctt
     4261 cgatacgttt gtcaaggatt tcgggcggat ggaaagccag atggccgagg tcgttcaaaa
     4321 tgtgtcgcag gtcaacgaga cgaatcattc catgagccgg accatttcaa gcattgccgc
     4381 gctcagctcg caggttcaga atcgtatggc caccatgaac gacgaagtga attcggtgcg
     4441 cgacaaaagt gaaagtttgc aggaattgct ggccacgctg cgcgcaggaa acacgccttt
     4501 cgactggctt tccggcatgc ttgattcgct tcgggatgct tgccgccgct tattgttgca
     4561 ggcgcgccag caaggggccg atatccttga cgtccgctat cagcggattc ccaatagcaa
     4621 tcccgctcgt ttccatacat cgtacgatac gttgattgat cagcaactga cacgaatact
     4681 ggactatgtg cttgaccagt tgccggcggg gtattatgcc ttgctggtcg ataagaacgg
     4741 ctactgtcca acgcacaata cgcgctattc gcgcgcctcc accggcgagc ttgcgcacga
     4801 taccttgtat gtgcgcaata agcgtatttt cgatgatcgc gtgggaaagc gcgccgccgg
     4861 caatacatcc gaaatcttgt gtcaaactta catgcgcgac accggcgaaa tcatcagcga
     4921 tttgtcaatt ccactggata tcgaccgtat gcattggggc gcagttcgaa ttggcatcga
     4981 ttacagccga ttcgaagaga ttcttcaggc caatgcataa cccagtctcc gttgggcgct
     5041 gatgcatgaa gtaagcggat ttatcggctt tgcttagtct atcgtattcc cctggctcat
     5101 ctagaatcta gtcagggttc cggccgccat gtgccggaac cgaagcagcg ccgattttcg
     5161 cggcatcatt gactatttcc cttattacga ctgaatggca gaggaaagcg acctcgagaa
     5221 aacagaagct gcaacgcctc agcggctgga gaaagcgcgc gaagaaggcc aggtggctcg
     5281 ttcgcgcgag ctcaacacgt ttctgctgct tgcagccggc gtgggtggac tgtggtttgg
//
LOCUS       DATCXU010000314         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_468442,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000314 DATCXU010000000
VERSION     DATCXU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468442"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VIR04_07035"
     CDS             <1..126
                     /locus_tag="VIR04_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY9460535.1"
                     /translation="GILPVVGVPLPFMSYGGTALSTLGVACGILMSISRYRPPRR"
     gene            complement(123..1226)
                     /gene="mutY"
                     /locus_tag="VIR04_07040"
     CDS             complement(123..1226)
                     /gene="mutY"
                     /locus_tag="VIR04_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY9460536.1"
                     /translation="MLLPPSAEPAPPIDDFASAVAEWQKRAGRHHLPWQGVGDPYKIW
                     LSEIMLQQTQVETVEGYYKRFLDRFPTVHALASATQDEVMPFWAGLGYYARARNLHRC
                     AQILAAQWEGQFPRRIEDIMQLPGVGRSTAAAIAAFAYGAREPIMDGNVKRVLTRYFG
                     IYGPPNQRATELALWRKAETIISVAPADLDIAAYTQGLMDLGSAYCTRTRPNCAQCPL
                     NLYCYARRESRQNELPTPKHKKISPERQCKMLILRNQDLVLLERQPSPGIWGGLWSLP
                     RYDDMDALNTACANWGQAIGEALKMPVISHTFSHFRLQIEPWQLVCRAPLAARPATQQ
                     AWVSIHDLATTALPSPIRKLLSGLAEDPPRNRR"
     gene            complement(1226..2134)
                     /locus_tag="VIR04_07045"
     CDS             complement(1226..2134)
                     /locus_tag="VIR04_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460537.1"
                     /translation="MNRWLRQHRYALRVALHRLSAHPFSSLSNLLVIALTLAIPIIGA
                     SILVSVQPVATQVAVSPEITLFLKHDAPSGADQKIADKVKTDYQQEIQQVRVAPREQA
                     LAALKAKPSWADALSVLPGNPLPDAVVVTLKDSPDLARHASLLAEQWRQWDQVDLVQL
                     DSNWVRRLEAIVQFGRIGLALLALGVALVVLATVFNTVRMQALTQREEIAVARLVGAT
                     EPFVRRPFLYLGALTGIAASLLAIVLSALALHPLNSALARLAASYGARLELQLPDSLS
                     MTLAVIVVAILAALSARWSVTRNTRF"
     gene            complement(2131..>2656)
                     /locus_tag="VIR04_07050"
     CDS             complement(2131..>2656)
                     /locus_tag="VIR04_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460538.1"
                     /translation="GLEPPSRGSVHVNGHRIDKMPMRRQRHDGRAVGAILQDTHLLYD
                     RNVIDNVLLPLTVIGLESSLAATRARAALEKVGLSGKDGLNPIEMSGGEQQRLAIARA
                     IVNRPAILIVDEPTANLDHESAQRIMDVFRDFNQVGVTTLIASHDRALMAAYATRTLL
                     IETDRFTDFQGVAA"
BASE COUNT          524 a          769 c          819 g          544 t
ORIGIN      
        1 ggaattctgc ccgttgtggg cgtcccattg ccttttatga gctacggggg cacggcgttg
       61 tcaacactgg gtgtggcgtg cggaatactg atgagcatca gtcgctatcg ccccccgcgt
      121 cgttagcgtc tgttgcgcgg cgggtcttca gccaaacctg acaatagttt cctgatgggt
      181 gacggcagtg cggtcgtcgc caaatcatga atggaaaccc atgcctgttg cgtcgccggc
      241 cgggcggcaa ggggagcgcg gcatacgagt tgccatggtt ctatctgaag cctgaaatga
      301 gaaaaggtat gcgatattac gggcattttc aaagcctctc cgatggcttg tccccaattt
      361 gcacacgctg tattcagggc gtccatatcg tcatatcgcg gcaggctcca caggcctccc
      421 catattccgg gcgacggttg ccgttctaat aaaaccagat cctgattgcg cagaatcagc
      481 attttgcatt ggcgttcggg gctgattttt ttgtgtttcg gggtcggcaa ttcattctgg
      541 cgggactccc ggcgggcata gcagtacagg ttaagcggac attgggcgca atttggccgg
      601 gttcgcgtgc aataagcgga cccaagatcc atcaggcctt gcgtataagc ggcaatatcc
      661 agatccgctg gcgcgaccga aataattgtt tcggctttgc gccataaagc cagctccgtc
      721 gcgcgctggt tgggcggccc gtaaatccca aagtaacgcg taaggacgcg cttgacattg
      781 ccgtccatga tcggttcgcg cgcaccatag gcgaaggcgg caatggccgc cgcggttgat
      841 cgcccaacgc cgggaagctg catgatgtct tctatacggc gtggaaattg cccttcccat
      901 tgcgcggcca atatttgcgc gcaccgatgc agattgcgag ccctggcgta gtagcccagt
      961 ccggcccaga aaggcatgac ctcatcttgt gtcgctgacg cgagcgcgtg gacagtgggg
     1021 aagcgatcca ggaagcgttt gtagtagccc tccacggttt cgacttgcgt ctgctgcagc
     1081 atgatctcgg agagccatat tttataaggg tctccgaccc cctgccacgg caaatggtga
     1141 cggcccgccc gcttttgcca ttctgctacg gcactggcaa aatcgtcgat cggaggcgcc
     1201 ggctccgcac tcgggggcag gagcatcaga accgggtgtt gcgagtgacc gaccagcgtg
     1261 cggaaagcgc cgccaaaatg gccacgacaa tcaccgcaag agtcatgctc aggctgtcag
     1321 gcagttgcag ttccaggcgg gctccgtaac tggccgccag gcgcgccaga gcggaattaa
     1381 gcggatgcag cgcaagcgcc gacaacacaa ttgccagcag gctggccgct attcccgtaa
     1441 gcgcgcccaa gtacaagaac ggccggcgca cgaacggttc ggtcgcgccg accaggcgcg
     1501 ccaccgcaat ttcctcgcgc tgcgtcagcg cctgcattcg cactgtatta aaaacggtcg
     1561 ccagaacgac cagcgcaacg cccaacgcca gcagcgccag accaatgcgg ccgaattgca
     1621 caatggcttc caggcgccgc acccagttgc tgtcgagttg caccaggtcc acctgatccc
     1681 actggcgcca ttgttctgcc agcaagctgg cgtgcctggc gaggtccggg ctatctttca
     1741 gggtaacaac gacggcatca ggcagcggat tgcccggcag caccgaaagt gcgtcggccc
     1801 aactgggttt ggccttgagc gccgcaagtg cttgttcgcg cggcgccact cgcacctgct
     1861 gtatttcctg ttggtagtca gtctttacct tatctgcgat cttctgatcg gcgcctgatg
     1921 gcgcatcgtg cttcaggaac agggtgatct cgggtgagac cgccacttgc gttgcaacgg
     1981 gctgaaccga caccaggatc gatgccccga tgatcgggat ggccagtgtt aacgcaatga
     2041 ccaataaatt ggacagggag gaaaagggat gggcggataa gcggtgcagc gccacccgca
     2101 acgcgtagcg gtgttgccgc aaccaccggt tcatgccgcc accccttgaa agtctgtaaa
     2161 gcggtctgtc tcgataagca acgtgcgcgt ggcgtaggcc gccatcaaag cgcggtcgtg
     2221 ggaggcgata agcgttgtga cgcccacctg attaaagtcg cgaaatacgt ccatgatgcg
     2281 ctgcgcgctt tcgtgatcca gattggcggt cggttcgtcc actatcagga tggcgggccg
     2341 gttgacaatg gcgcgtgcaa tagccaggcg ctgttgttcg ccgccggaca tctcgatggg
     2401 attcaggccg tctttgcctg aaaggccgac tttttccagc gcggcgcgcg cgcgggtggc
     2461 ggccagactg ctttccaggc cgattacggt taatggaagc aacacgttgt cgatgacatt
     2521 gcgatcgtac agaaggtgag tgtcttgcaa tatggcgccg acggcgcggc cgtcgtggcg
     2581 ttgacggcgc atcggcattt tatcgatgcg atgcccgttg acgtgcaccg agccgcggct
     2641 tggcggctcg agtccg
//
LOCUS       DATCXU010000315         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_248439,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000315 DATCXU010000000
VERSION     DATCXU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248439"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1777)
                     /gene="argS"
                     /locus_tag="VIR04_07055"
     CDS             complement(89..1777)
                     /gene="argS"
                     /locus_tag="VIR04_07055"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY9460539.1"
                     /translation="MLPGQQQQLIELIRTTVAKIIPGAAPTILLERPKVAAHGDMATN
                     VAMQLAKIAHRNPRELAQQIVDALRPELDAGELIASSEIAGPGFINFRLTAAANQAVL
                     DAISEQGKHYGTLPATQKKVMVEFVSANPTGPLHVGHARQAALGDSICRLFTSQGYDV
                     TREFYYNDAGNQINNLAISVQARANGVDPDSAGFPLDGYKGDYIADIARDFLAGKTIA
                     ATDSEPAVTATGNIDNLDDIRRFAVAYLRKEQDLDLQAFDLKFDNYFLESSLYTSGRV
                     ENTVKTLAAAGYTYENEGALWLRTTALDTGDDKDRVMRKSDGTFTYFVPDVAYHVTKW
                     ERGFHHAINIQGSDHHGTIARVRAGLQGLNIGIPKDYPSYVLHKMVKVVRNGVEVKIS
                     KRAGSYVTLRDLIDWVGQDAVRFFLIQRRADSEFVFDIDLALSKSEENPVYYIQYAHA
                     RICSILGQSKDLALEFIHAATRKLLAPTELALMRRLAEFPDTVTLATHELAPHHVAFW
                     LRDCAADFHAWYNAERVLVDDVELKLARLRLAQATRQVLASGLELLGVSAPERM"
     gene            complement(1934..>2839)
                     /locus_tag="VIR04_07060"
     CDS             complement(1934..>2839)
                     /locus_tag="VIR04_07060"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY9460540.1"
                     /translation="FYHSGPGNPGQFDWAAISAYNAPDIIEAQWQSLLTELHDNAGPR
                     DTIPIDIIGFSRGAALARHFGNVIDEHVNQGYFSYTDPSRGLITACVDLRFMGLFDTV
                     AQFGLLGMQNANYDLTIASAWEWVAHAVALQERRWLFPLTSAAGGTGRNTVEAPFIGA
                     HSDIGGGLAFDDAGNVSSRGDLSDVALNWMLWQARAAALRFGEIDPADREITEPILHD
                     QSAALVRSSQKGDRRIDAPDGSILNNQQNDHARLGRQQRAATERLITRGDNWQHSIAI
                     EVGVVDMAGYAQWLHDELGWQAIPA"
BASE COUNT          566 a          781 c          863 g          629 t
ORIGIN      
        1 ccgccacacc aatgattaaa cccgcgagca gcccgtacaa gcctctaccg ctggatcctt
       61 gacgattttt tcgggccata tgtgcactct acattctttc gggggcggat acaccaagca
      121 attccaggcc gcttgccaga acctggcgcg tcgcctgggc caggcgcaag cgcgcgagtt
      181 tgagttctac gtcgtccacc agcacgcgct cggcgttata ccaggcgtgg aagtcagcgg
      241 cgcaatcacg cagccagaat gcgacgtggt gcggtgcgag ttcgtgggtt gccagcgtca
      301 ccgtgtccgg gaattcagcc aggcgccgca taagcgccag ctcggtaggg gcgagcagct
      361 ttcgtgtggc ggcgtgaata aattcaaggg ccaagtcttt ggactggccc aggatggagc
      421 aaatgcgcgc atgcgcatac tgaatatagt acaccggatt ttcttcgctt ttcgacaagg
      481 ccagatctat gtcaaagacg aattcggagt cggcccgccg ctggatcagg aaaaagcgca
      541 cggcatcctg gccgacccag tcgatcagat cgcgtagcgt gacgtaactg ccggcccgct
      601 tggaaatttt cacttcgacg ccattgcgca ccaccttgac cattttatgc agcacgtacg
      661 agggatagtc tttcggaatg cctatgttca gtccttgcaa acccgctcgc acgcgagcga
      721 tggtgccatg atgatcgctg ccctggatgt tgatcgcgtg atgaaagccg cgctcccatt
      781 tggtgacgtg ataggccaca tcgggaacga agtaggtgaa agttccgtcc gacttgcgca
      841 tgacgcggtc tttgtcgtcg cccgtatcca gcgccgtggt gcgcagccac agtgcgcctt
      901 cgttttcata ggtgtagccc gcggcggcca gcgtcttgac ggtgttttcg acacgtccgc
      961 tggtgtagag cgaactttcc agaaagtaat tgtcgaattt cagatcgaac gcctgcaggt
     1021 cgagatcctg ctctttgcgc agataggcga cagcaaagcg gcggatatca tcaagattat
     1081 cgatattgcc ggttgcggtg acggcgggct cggaatcggt ggcggcaatg gttttgcctg
     1141 cgagaaagtc gcgtgcaata tccgcgatgt agtcgccttt gtagccatcc agcgggaacc
     1201 ccgccgaatc cggatcgacg ccattggccc gcgcctgaac gcttatggcc agattgttga
     1261 tttgattgcc tgcgtcgttg tagtagaact cgcgcgttac gtcgtagcct tgcgatgtaa
     1321 acaggcggca gatcgaatcg cccagggctg cctggcgcgc atgtccgaca tgaagcggac
     1381 cagtcggatt ggccgaaacg aactccacca tcactttttt ttgtgtggcg ggcagcgtgc
     1441 cgtaatgctt gccttgctct gaaatggcat ccagcacggc ttgattggcc gcggccgtaa
     1501 ggcggaagtt aatgaacccc ggcccggcga tttcgcttga ggcgatgagt tcaccggcat
     1561 cgagctcggg ccgcaaggcg tcgacaattt gttgggccag ttcgcgcggg ttgcggtgcg
     1621 cgattttggc cagttgcatg gcgacattgg ttgccatatc gccatgcgcg gcaactttcg
     1681 ggcgttcgag caagatggtt ggagcggccc ccggaataat tttggccaca gttgttcgaa
     1741 tgagctcgat aagctgctgt tgttgccctg gaagcatagt tgttgttttt gaatcggaga
     1801 ttgaaaagag attgcaggcc aggcagtaaa tagcgttttt tttgggcgct agcggcccgc
     1861 aaaagccaca cgacacaaat gatagcgtga tttcccgtgt gacgtattgc gtccgttcga
     1921 tacttggccc ggctcaggcg ggaattgctt gccatccaag ttcatcgtgc agccattgcg
     1981 cgtagccagc catgtcgacg acaccgactt cgattgctat gctgtgttgc caattatcgc
     2041 cgcgtgtaat aagcctttcg gttgcggcgc gctgctgccg gcccaggcgc gcatggtcat
     2101 tttgctggtt attcaggatc gagccgtccg gagcatcgat gcgacggtcg cctttttgcg
     2161 acgaccgcac cagagcggcg ctttggtcgt gcaaaatcgg ttctgttatt tcgcgatcag
     2221 ccggatcaat ctcgccaaag cgtagcgcgg cggcgcgcgc ctgccatagc atccagttta
     2281 gcgccacgtc ggataaatcg ccgcgcgagg atacattgcc ggcgtcgtcg aacgcgagtc
     2341 cgccgccgat gtcggaatgg gcgccgataa agggcgcttc gaccgtgttg cggccagtgc
     2401 cgccagcggc cgaggtcagg ggaaacaacc agcgccgttc ttgcagcgca accgcgtgcg
     2461 ccacccattc ccatgccgag gcgatagtca ggtcatagtt ggcgttttgc atgcctaata
     2521 acccgaattg agcgaccgtg tcgaacaggc ccataaagcg cagatcgacg caggcggtga
     2581 tcaggcctcg ggatgggtcg gtatatgaaa agtaaccctg atttacgtgt tcgtcgatga
     2641 cattgccgaa gtgccgcgcc agggcggctc cgcgcgaaaa gccgatgatg tcgatgggaa
     2701 tcgtatcgcg cgggccggca ttgtcgtgca gctcggtcag caaagattgc cattgcgcct
     2761 cgattatgtc gggtgcgtta taagcgctga tggcggccca gtcgaactgg cccggattgc
     2821 ctggaccact atgataaaa
//
LOCUS       DATCXU010000316         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_35616,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000316 DATCXU010000000
VERSION     DATCXU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35616"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIR04_07065"
     CDS             complement(<1..199)
                     /locus_tag="VIR04_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460541.1"
                     /translation="MSATTVSPPQGASGNLIQFISMAGSTDLRAAVVEKTKQHIVDTM
                     AAMVSGIALNVGIRAMAVAPAL"
     gene            complement(456..1181)
                     /locus_tag="VIR04_07070"
     CDS             complement(456..1181)
                     /locus_tag="VIR04_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9460542.1"
                     /translation="MIKKSVNTGSRETSEADFGQSLQPFSLSDRAYQRIEAIILSGKL
                     KGGERLNDSKLAKAFSVSRGPVRNALARLAEAGLVELIPYRGAFVRTIDFNDVLEIYE
                     VRAAVERAGILAACRNMTPEVLAKLGGLVAAMNESFKKGDKEQYFKGNLAFHELIHQT
                     SGNSRLLELYQRYTREQKLFRHFSLTTVGIEESNKQHSRIMRALEAGDSEAAAREMEK
                     HVLSAKHRLEQSVKKIKEEYLAI"
     gene            1389..2822
                     /locus_tag="VIR04_07075"
     CDS             1389..2822
                     /locus_tag="VIR04_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460543.1"
                     /translation="MPQQNSLTQQVTQFIENIQYADLPQESIGIASRCMLDTFGLYVT
                     GATEPSVRILVEDAQATGGRPDATLLSGGGNKVPAGLAARVLGTAGHSQDWDDTQVSL
                     DPKHRYGMLTHPSVPPFTAALVIAQYLGGISGKQLMLAFQTGFEVECKISECMLPDHY
                     RRGHHSSGTVGTFGACAAAAKLLGLKGEKLANAIGIATSLAAGVRCNFGTMTKPLHVG
                     RAAENGITAAFLAQRGFTADPASLDGPWGFMQVLGGGHTPEKVAAGLGKPLTIVSPGV
                     SIKPYPSGILTHQAMDALLELVLEHDLKPDQIRKIKFYAASNILEPIRYTLAKNHLQA
                     KFSMQALLAMIVLRRHAGPHEFTDEFVGGAEMQNMQQRIETIHDPEIEAQGFEWIRSR
                     FILETTDGRTLEKEADKRYRGGPSKPMTNAEVEDKFRICTDGLLNPQKQKDIVAAIWS
                     IGTLEDSSMLLDMLGKIATTDSNGAHK"
     gene            2819..3874
                     /locus_tag="VIR04_07080"
     CDS             2819..3874
                     /locus_tag="VIR04_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY9460544.1"
                     /translation="MNRESENRYPKIKVAAVQAGPVFMDRDETVDKACRLIQEAGSNG
                     AQLVAFPEAFIPAYPYWSRYLPPMQSMRYTKELIKQAVQVGSPTTERLTRAARDAGIH
                     VVIGINEKVAHAHGTLFNTNLLIGPTGQILGRHRKLVPTLSEKLVWAYGDGEGLQTHE
                     TELGRLGSLICGENANPLARYVLIADGEQVHVANYLALPKKDTGGYNLAKDIEIRSAA
                     HSFEGKIFTVVSSLVINQSVIDYFSGQDELQALLSSGSVGHTAVYGPWGLPVAGPMEA
                     GIEGILYADIDLEDALLPKLRHDIGGGYNRFDIMRVLVNRNPHRSLQDSMGAARQSEQ
                     AASDGVASKPEPRQAEN"
     gene            complement(3859..>4514)
                     /locus_tag="VIR04_07085"
     CDS             complement(3859..>4514)
                     /locus_tag="VIR04_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9460545.1"
                     /translation="HGFANTRDGDFDAMLDGFGKEWIWTTLAFKPYACGTMAHPFIDC
                     AREFRKKGIDPESIDLIECDTAEGIVHRLWEPMALKCAPPNGYAAKFSIPYAIAVAIL
                     RDDAGLGDYDDAVVKNPELLRLTGKISYRIDPENPYPDRFIGHIRVVLKNGVVHEHRQ
                     DFFRGGAGHPLSMKALEDKFLANCEFGGVSRNRAQAMADGIHALFDTSELNFSQFSA"
BASE COUNT         1101 a         1261 c         1175 g          977 t
ORIGIN      
        1 tcaacgccgg tgcaacagcc atcgcgcgaa tgccgacatt cagcgcgatg cccgagacca
       61 tggctgccat agtatctacg atgtgttgct tcgttttttc tactacagcc gcacgcaaat
      121 cggtgctgcc agccatggag ataaattgaa tcaaattgcc ggacgcgcct tgaggcgggc
      181 tgacggttgt cgcggacatg aagcggccct tgcaagaaag atcagtaaat cttattccgg
      241 cgccttggct cttgcgtgtc tataaggcta ttttatcgac ggccggccca ggaaaatgaa
      301 gagtacttta ttgtcggatg caaattgttt attgtcaaca gaattaacgc gcgcgaaatc
      361 aagccgcgcc tccattgaaa caggcccgct tccgggcctt gatcaggtat tgcttgtcag
      421 aggccatgcc agaacccggc atgcgcacgc gcttatcaaa tcgccagata ttcttctttg
      481 atctttttga cggactgctc cagccggtgc ttggccgaga gaacatgttt ttccatttcg
      541 cgggcagcgg cctcgctgtc gccagcctcg agcgcgcgca tgatccgtga atgttgcttg
      601 ttcgattctt cgatccccac tgtagtcaac gaaaaatgcc ggaacagctt ttgctcgcgc
      661 gtgtatcgct ggtaaagctc gagcaggcgg ctatttccgc ttgtttgatg aatcaattcg
      721 tggaaagcca gattgccctt gaaatattgc tctttgtcgc cttttttgaa gctttcattc
      781 atcgcggcga caaggccgcc cagcttggcg agtacttccg gcgtcatatt gcggcaagcc
      841 gccaaaatgc cggcgcgttc aacagccgca cggacctcgt agatttccaa aacatcgttg
      901 aagtcgattg tccgcacgaa tgcgccgcga tagggtatca gttccaccaa ccccgcctcg
      961 gccaaacgtg ccagcgcatt cctgacaggg ccgcgactca cagaaaatgc tttagccaac
     1021 tttgaatcgt tcagtcgttc gccgcccttt agcttacccg acaaaataat tgcttcaatc
     1081 cgctgataag cgcggtcaga caatgaaaaa ggttgcaaag actgaccgaa gtctgcttcg
     1141 ctcgtttcac gagagcctgt attgactgat tttttaatca tccgttcaca ctgctaagtt
     1201 gctaaattgt tgttttaaaa gatgaaacag atattactgt atggcagacc gttcgtcact
     1261 gaaaacctga atgttgacaa taaacaatat acatcacaca atcgaaataa gcagcaataa
     1321 atcatggcga aaacacgctc gttaacgtgg ttgttaacgg ggcgccgatc cctttacgca
     1381 ggaaaactat gccgcaacaa aactccctta cgcaacaggt gacccagttc atcgaaaaca
     1441 tacagtatgc cgatctcccg caggaatcga ttggaatcgc aagccggtgc atgcttgata
     1501 cattcggtct gtacgtaacc ggcgctacag aaccgtcggt gcgaattctg gtcgaagacg
     1561 cgcaagccac ggggggccgt ccggatgcga cgttgctatc gggtggcggc aataaagtgc
     1621 cggcgggcct cgcggcgcgc gtactgggca cggcgggtca ttcgcaggac tgggatgaca
     1681 cccaagtaag ccttgatccc aaacatcgtt atggcatgct cacccatccg tcagttcctc
     1741 cctttaccgc cgctttggtg attgcgcagt atttgggcgg catatcgggc aaacaactga
     1801 tgctggcatt ccagacggga ttcgaggtcg aatgcaagat ctcggaatgc atgctgcccg
     1861 atcattaccg gcgcggacat cacagcagcg gcacagtagg cacattcggc gcttgcgcgg
     1921 cggccgcaaa actattgggc cttaaaggtg aaaaactggc caatgcgatc ggaatcgcca
     1981 ccagcctcgc ggcgggcgtg cgctgtaatt tcggcaccat gaccaagcct ttgcacgttg
     2041 gccgggccgc cgaaaacggc ataacggcgg catttctggc acagcgcgga tttaccgcgg
     2101 atccggcgtc gctggacggc ccgtggggtt tcatgcaagt gcttggcggc ggacacacac
     2161 cagaaaaagt agcggccggg cttggcaagc cgctgaccat cgtctctccc ggggtaagca
     2221 tcaagcctta tccctcgggc attctgactc atcaggccat ggatgccctc ctcgaacttg
     2281 ttctggaaca cgacctgaag ccggaccaga tcaggaaaat caagttttat gccgccagca
     2341 atattctcga acccatccgt tacacgctgg caaagaacca tctgcaagcc aaattttcca
     2401 tgcaggcact attggcgatg atcgtactgc gccgccacgc tggcccgcat gaattcaccg
     2461 acgaattcgt tggcggcgcc gagatgcaaa acatgcaaca gcgcatcgaa accatacatg
     2521 atcccgaaat cgaggcgcag ggcttcgaat ggatccgatc caggttcata cttgaaacaa
     2581 cggatgggcg cacccttgaa aaagaagcgg ataagcgtta ccgcggtggt cccagcaaac
     2641 ccatgacgaa cgccgaagtt gaagacaaat tcagaatctg caccgatggg ctgttgaacc
     2701 cacaaaaaca aaaggacatc gtcgccgcaa tatggtctat tggaactctc gaagacagct
     2761 caatgttgct tgatatgctg ggaaaaatag ccacgacaga ctcaaacgga gcgcataaat
     2821 gaatagagag tcagagaatc gttacccaaa aatcaaagtc gccgccgtgc aggccggccc
     2881 ggttttcatg gatcgggacg aaaccgtcga caaggcatgc cggctgatcc aggaagcggg
     2941 aagcaacggt gcgcaactcg tggctttccc cgaagcgttt atcccggcat atccctattg
     3001 gtcgcgctac ttgcccccga tgcaatccat gcgctacacc aaagagctca tcaaacaggc
     3061 ggttcaggtg ggcagcccga caacagaacg cctgacccgg gccgcccgag acgcagggat
     3121 tcatgtcgtt atcggcatca acgaaaaagt cgctcatgcc catggcacgc tgtttaacac
     3181 caacctgctt atcgggccaa ccgggcagat tttggggcgc cacagaaagc tggttccgac
     3241 cctgtctgaa aagctggttt gggcctatgg cgacggcgaa ggtttgcaaa cgcatgaaac
     3301 ggaattgggt cgcctgggca gccttatctg cggagaaaac gccaacccgc tggctcgtta
     3361 cgttcttatc gccgacggag agcaagttca tgtcgccaat tacctggcgc tgcccaagaa
     3421 agacacgggc gggtacaacc tggcgaaaga catcgaaatc cgtagcgcgg ctcattcctt
     3481 cgagggaaag attttcaccg ttgtttcttc gctggtcatt aatcagtcgg ttatcgacta
     3541 tttttctggc caagacgaat tgcaggcgtt gttatcaagc ggcagtgtcg gccacaccgc
     3601 cgtctatgga ccctggggtc tgcccgtggc gggtccgatg gaagcgggca tcgaaggaat
     3661 cttgtatgcc gacatcgatt tggaggacgc cctgcttccc aagctacgcc atgatattgg
     3721 cggaggctac aaccgttttg acatcatgcg ggtgctggtg aaccgtaacc cgcaccgatc
     3781 gctgcaagat tctatgggcg ccgcgcgcca atccgaacag gccgcttcgg atggggtcgc
     3841 ttcaaaaccc gaaccccgtc aagccgaaaa ttgagaaaaa ttcagctctg acgtgtcaaa
     3901 cagtgcatga atgccatcgg ccattgcctg cgccctgttc ctggaaacgc cgccgaattc
     3961 gcaattcgcc aaaaatttat cctcaagcgc tttcatggac aatggatggc cggcgccacc
     4021 cctgaagaag tcctgccgat gctcgtgaac cacgccattt ttcaggacga cccgaatgtg
     4081 tccgataaac cgatccggat aagggttttc cggatcaatg cgatacgata ttttaccggt
     4141 aagacgcaac agctcagggt tttttacaac cgcatcatcg taatccccca agcccgcgtc
     4201 gtcgcgcaat atcgcaacgg caatagcgta aggaatacta aattttgcag catagccgtt
     4261 gggcggcgcg cacttcaaag ccatcggctc ccacaagcga tgcactattc cctcggcggt
     4321 atcgcactca ataaggtcga ttgattcggg atcaattcct tttttccgga actcgcgcgc
     4381 gcaatcgata aatgggtggg ccatcgttcc gcaggcatac ggtttaaatg caagcgtcgt
     4441 ccaaatccac tctttcccga aaccgtccag cattgcgtcg aaatcgccgt cacgcgtatt
     4501 ggcaaacccg tgaa
//
LOCUS       DATCXU010000317         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_425220,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000317 DATCXU010000000
VERSION     DATCXU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425220"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VIR04_07090"
     CDS             <1..481
                     /locus_tag="VIR04_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460546.1"
                     /translation="VPHARPAKRWLLKVAAVLVSSACCIGSAWPQGYHADDLAQDQIS
                     VRELMRLDTALALSQAKDKLHSRHKATPAADSQGLPAHSGRLRLVAIYGVGKKLLAEV
                     HIGSKPHVYMRGKALPVGVAADPTSFQLRGITGSCVRLERKDESHTLCLHPALWVQG"
     gene            448..2235
                     /locus_tag="VIR04_07095"
     CDS             448..2235
                     /locus_tag="VIR04_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY9460547.1"
                     /translation="MLAPRFVGTGVMVMRAPWTRSLLPQSPQARAAPVRQTTTRFDAS
                     QAVAISGPDELMALEPAFLKSLSSEFNVEALAGRLCPVLLGDHSVAIFSLGEHVGSDQ
                     ADELARCVGQSGYALADPQRYVLAAPLLLAIARNQVTAQSLTNQSGRYREQSKTALAD
                     AFQDLIEWGVRHGASDLHLNVRLREPESEVKATLAGRYVAPERFQRMPTTTLIDMLSV
                     AWMDIQGGNGAVFDPSSEQQGQMIRLVDGKTVLLRWASLAADQGPSVCLRLLRYDAAA
                     SYPSLEKLGYLPDQVSVIERAMLAEGGAIVFAGTVGSGKSTTLASLIAGLADSRKVIT
                     IEEPVEYLIPRAIQNTVTRDLDATAHNVYAAKLRALKRSAMNDVLLGEIRDTETGRAF
                     MDLAGSGINVYTTTHAGSAALICERLASDFIGVSRDFLATRNILKLLVHQSLLPKLCQ
                     QCALKATHLLKGAGGADGRYHAGGHWRMWLNRIQRLYDCDIELLRIRNPQGCSACHVT
                     QAPSLSGYAGRTVVAEHIEPALTPGFLESIHDADGSVRVTRTAMQCAMVKAMRGQIDP
                     REIELRFQPFETQELLHDMRRDSACGASA"
     gene            2232..3359
                     /locus_tag="VIR04_07100"
     CDS             2232..3359
                     /locus_tag="VIR04_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460548.1"
                     /translation="MNSLFHTTRQDANQLLVYIDAWRFAPRRADYYDYLSCLMDGMQG
                     SRTLKDIFQADARRYGPCTVRGRLSLRWANAYQAFGGDLYMTWLGCFPLAELSVIRAA
                     QAFGNTALTATLRDLAEAADLAQRAKELLFSTLWAAALSVIALLAMVLAVPLFTVPRL
                     LHTFYSVPSELYGPLTRSLIAFSIFVQAQWLFVLVLVLGGGALIVWSLPNLSGAARRR
                     LDKFLFWRSYRHVNAMRFLALLAIILARQGSVSTQLRTALSMQKTGSSRWQCWQIDAM
                     LERINMGQAGAETFDTGLLDQDMFWFLSDMAAARGLAAGLSLTRRRLENRLLATVARH
                     ALALRWCSLLFCLACLLGLGLWHYAVIDELRRSLMLFYASQ"
     gene            3387..3971
                     /locus_tag="VIR04_07105"
     CDS             3387..3971
                     /locus_tag="VIR04_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HEY9460549.1"
                     /translation="MSAITSVAPFHPLRRQQGFSLIEVSIVTAIVLLLAIISIPAIGS
                     YVVENKVPKVGEELVRFILQTKVNAPNSSTTPYEGIDTGNFANLVRESSIFSVSGVDA
                     STRVLHGLGSDGEVVVSPEGTGASFSITLSKVNNSACPSIASIMQRVADSITVSAQGQ
                     SAATVKDDTTAYSALTTESKCARGDVNTFVFTAS"
     gene            3981..>4558
                     /locus_tag="VIR04_07110"
     CDS             3981..>4558
                     /locus_tag="VIR04_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460550.1"
                     /translation="MQRQRGFFLFELIVVVLIATLLAVWSSDALVNRVNDSAAQASAT
                     WMLSVRKTAQAYIERYADTLKNAADPQALIHKGYADWSAPSLAELKADNLLSPGFPER
                     GAGGLQVVVRILRSGICPDAACRLEALIHSAQAVTRRQTRYIDESMIAQWLMATRGWG
                     GAVTRFRPDQVAGAAFALPNPPAADIGTLPVGT"
BASE COUNT          875 a         1195 c         1384 g         1104 t
ORIGIN      
        1 cgtgccgcac gcacgtccgg ctaagcggtg gctgcttaag gtcgccgccg ttttagtttc
       61 gtcggcgtgc tgcattggct cggcctggcc tcagggctat catgccgacg atctcgcgca
      121 ggatcaaata tccgtgcgtg aactgatgcg gctggacacg gctttggcgc tcagtcaggc
      181 aaaggacaaa ctccattccc gccacaaggc aacgcctgcg gctgattctc aaggcctgcc
      241 ggcgcatagc gggcgtcttc gcctggttgc catttatggc gtcggaaaaa agctgcttgc
      301 cgaagtgcac atagggtcga agccacatgt ctatatgcgc ggcaaggcat tgccggttgg
      361 tgtcgccgct gatcctacgt cctttcagtt gcggggaata acaggttctt gtgtacggct
      421 ggagcgcaag gatgagtcac ataccttgtg cttgcacccc gctttgtggg tacaggggtg
      481 atggttatgc gcgcgccatg gacacgttcg ttgctgccgc aatcgccgca ggcgcgggcg
      541 gcgccggtac ggcagaccac cacacgtttt gatgcgtcac aggcggttgc catatcgggg
      601 ccggacgagc tgatggctct cgaaccggct tttttgaagt ccttaagttc cgaattcaat
      661 gtggaggccc tggcaggcag gttgtgtccg gtgctattgg gtgatcattc ggtggctata
      721 ttttctcttg gcgaacatgt aggcagcgat caggccgacg agctggcgcg ctgcgttggc
      781 caatccggct atgcactggc tgatccacaa cgttatgtgc ttgccgctcc actgttgctg
      841 gccattgcgc gtaatcaagt tacggcgcag tctctgacga accaaagcgg ccgatatcga
      901 gagcaatcga aaaccgcatt ggccgacgcc ttccaggact tgattgaatg gggtgttcgg
      961 catggcgcaa gtgatctgca tttgaatgta cgcttgcgcg aacccgagtc agaggtaaaa
     1021 gccacgttgg caggacgtta tgtcgccccc gagcgatttc aacgcatgcc aaccactacg
     1081 ctgattgata tgttgtcggt tgcctggatg gacattcagg gaggcaatgg cgcggtgttc
     1141 gatccatcat ccgagcaaca aggacaaatg atcagactgg tcgatggaaa aacagtgctg
     1201 ctgcgctggg catcgctggc ggctgaccag gggccgagcg tgtgcttgcg tttgttgcgc
     1261 tatgacgccg cggcgtccta tcccagtctg gagaaattgg gatatttacc ggatcaggtg
     1321 agcgttatcg agcgggccat gctcgccgaa gggggcgcaa tcgtttttgc cgggacggtg
     1381 ggatcgggca agtccacaac gctggcttcg ctgatcgcag gtctggcgga tagcagaaaa
     1441 gtcattacca tcgaggagcc ggtcgaatat ctgattccgc gtgccatcca aaatacggtt
     1501 acgcgcgact tggatgcaac cgcccataac gtctatgccg cgaagttaag ggcattaaag
     1561 cgctcggcca tgaacgatgt gctgctaggt gaaatccgcg atacggaaac ggggcgggct
     1621 tttatggatt tggcagggtc gggaattaat gtctacacaa cgacgcatgc gggatcggcg
     1681 gcgctgattt gcgagcgtct ggcgtcggat ttcattggtg tttcgcgcga tttcctggcg
     1741 acgcggaata tattgaagtt actggtgcat cagtcgctgt tgcccaagtt atgccagcaa
     1801 tgcgccttga aagccaccca tttattgaag ggagccggcg gcgcggatgg acgctatcac
     1861 gcgggcgggc actggcggat gtggctaaat cggattcagc ggctctatga ctgcgatata
     1921 gaactgttgc gtattcgcaa tccgcaaggt tgttcagctt gtcatgttac acaggctcca
     1981 agccttagcg gctatgcggg gcgcacagtt gtggccgagc atatcgaacc cgcgctgacg
     2041 cctggctttt tggaaagtat tcacgatgcc gatggctccg ttcgcgtcac tcgcacggca
     2101 atgcaatgtg cgatggtgaa agcaatgcgc ggtcagattg atccgcgcga gatcgaactg
     2161 cgctttcaac catttgaaac acaagagctg ttgcacgata tgcgccgtga ctcggcttgt
     2221 ggagcatcgg cttgaattcg ctctttcata cgacgcggca ggatgcgaat cagcttctgg
     2281 tttacatcga tgcatggcgc tttgcgccgc gccgcgccga ctactacgat tatttgtcct
     2341 gtctgatgga cggcatgcaa ggcagtcgaa cgttgaaaga tatttttcag gcagatgcgc
     2401 gccgttacgg gccctgcacc gtgcgtggac gcctttcact tcgttgggcc aatgcctatc
     2461 aggccttcgg cggcgacctc tatatgacat ggcttggctg ctttccactg gccgaattaa
     2521 gcgtaattcg cgccgctcag gcatttggaa atactgcgct cacggcaacc ctgcgcgatt
     2581 tggccgaggc ggcagacctg gcgcaacgcg ctaaagaact gttgttttca acgttgtggg
     2641 ccgctgcgct gtccgtcatt gcgctgctgg ccatggtgtt ggcggttcca ttgttcacgg
     2701 ttcctcgctt gttgcatact ttttacagtg tgccgtcgga gctctatggc cctttgacgc
     2761 ggtcacttat agcgttctcc atattcgtcc aggcacagtg gctgtttgta ctggtgctgg
     2821 tgttgggcgg aggtgcgctt attgtgtggt ccctgcccaa tttgtcgggc gccgcccgcc
     2881 ggcgccttga caagtttctt ttttggcgaa gctatcgcca tgtgaatgcc atgcggttct
     2941 tggccctgct ggctattatt ctggcgcggc aaggctcggt atcaacccaa ttgcgcacgg
     3001 ctttgtcaat gcaaaagacc gggtcttcca gatggcaatg ctggcagatc gacgccatgc
     3061 tggaacgaat caacatgggt caggcggggg ccgaaacttt tgatactggc ttgctcgatc
     3121 aagatatgtt ctggtttctg agcgatatgg ctgcggcgcg cggccttgcc gcaggcctta
     3181 gcctgaccag acgaagactt gaaaaccggc tgcttgcgac agtggcccgg catgctctcg
     3241 cattgcgctg gtgctcactc cttttttgcc tcgcctgtct gcttggcctg gggctttggc
     3301 attacgccgt catagatgaa ctgcgccgat ctctcatgct tttttacgcg agccaataaa
     3361 gggtcgtgtc tttttaagga accgtcatgt ctgctattac ctcagtcgcc ccattccatc
     3421 cgctgcgccg ccagcaaggc ttttccctga ttgaagtctc aatcgtaacg gcaattgtgc
     3481 tgctcctggc gataatcagt attccggcta ttgggagtta tgtggttgaa aacaaggtgc
     3541 ctaaagtagg cgaggaatta gtacgattta ttttgcagac caaagtcaat gcgccaaaca
     3601 gttcgaccac tccttatgaa gggattgata ccggcaactt cgccaacctg gtgcgcgagt
     3661 cgagtatttt ttcagtgtcg ggcgtcgatg caagcacccg ggttctgcac ggtttgggca
     3721 gcgatggcga agtggttgtc agtccggagg gaaccggcgc ttcgttttcg atcaccttgt
     3781 ccaaggtgaa caactcggca tgtccttcga tagcatctat catgcagcgt gtcgctgatt
     3841 cgatcaccgt gtccgcgcag gggcagtccg cagcgacagt gaaggacgat acgacggcgt
     3901 atagcgcgct gacgactgaa tccaagtgcg ccagggggga tgtcaacaca ttcgttttca
     3961 cggcaagcta agggacggcc gtgcaacgtc aacggggatt ctttctgttc gagctgatcg
     4021 ttgtggttct gattgcaact ttgcttgcag tatggagcag cgacgcgtta gtcaaccggg
     4081 tcaatgattc ggcggctcaa gcgtcggcga cctggatgct atcggtcagg aaaacggcac
     4141 aggcatatat cgagcgttat gccgacacgt taaagaatgc tgccgatccg caggctctga
     4201 ttcataaggg ttacgccgat tggtcggctc cgagcctggc tgagcttaaa gcggataatc
     4261 tgttatcccc aggctttcct gaacgcggag cgggtggtct gcaggttgtt gtgcgtatcc
     4321 tgcgtagcgg catctgtccc gatgctgcgt gccgacttga ggcgctgatt catagcgccc
     4381 aggccgtgac gcggcgtcaa acgcgttata ttgatgagtc gatgattgcg cagtggctta
     4441 tggcgacgcg gggctggggg ggcgcggtaa ctcgtttccg gcccgatcag gtggcgggcg
     4501 ctgcgtttgc cttgccgaat cctcccgcgg ccgatatcgg cactcttccc gtggggac
//
LOCUS       DATCXU010000318         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_394761,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000318 DATCXU010000000
VERSION     DATCXU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394761"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VIR04_07115"
     CDS             <1..472
                     /locus_tag="VIR04_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator TrmB"
                     /protein_id="HEY9460551.1"
                     /translation="PQLRSMYNRATGKPQIRFYEGEQGIITALWDTLTVTSEPKVLRG
                     ILSMDELMQTPGMAEMERFIANRVQRDIWLKVIRSEQKDVDAIWPSNVEKLRDLRYAP
                     PEMLLSMTTFIYDHRVCLISSKKENYGLIIESEEFSTMQTVLFDGIWGLSKAPD"
     gene            complement(527..1372)
                     /locus_tag="VIR04_07120"
     CDS             complement(527..1372)
                     /locus_tag="VIR04_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY9460552.1"
                     /translation="MIVPFLDLVCPLDGVALRRTGDTWGCASGHSFDVARQGYVHLLP
                     VQKKHSRDPGDSKQMVAARRRFLSAGFYQPIAAMVGKAVLADLPSGITVNCLDAGCGD
                     GYYLHQLAAEAGDEQTLALLGLDISKWAILSAAKHDKRSSWVVGSNANLPVRARSLDR
                     VLCMFGFPVYAEFARVLRPGGQLLQVDAGSDHLRELREIIYPTLKPKDGVRAQTPEGF
                     ADLPGEHIRYSLSLDSKEQIADLLMMTPHLYRASAEGRAKAAALTALSVTVDVSLTRF
                     VAEPH"
     gene            1566..2576
                     /locus_tag="VIR04_07125"
     CDS             1566..2576
                     /locus_tag="VIR04_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY9460553.1"
                     /translation="MNEYASAEKILQLGTAFWASKTLLSAVELKLFSELAKKPLDHGA
                     LAAKLGLGSRGTLDFFDALVALGMLEIHNGKYCNTPEAALFLDASKETYIGGILEMAN
                     DRLYPFWGRLTEALKTGQPQNEAKSGENTFATLYADPARLKQFLQAMTGVSLANATAL
                     AQEFPWSNYQSFADIGCAQGAVPVQVAKFHPHLSVAGFDLPSVRPIFEEYVQSHGLTA
                     RVRFISGDFFNDPLPHVDVIVMGHVLHDWDLDQKRTLLNKAYASLPKGGALIVYDAMI
                     DNERKKNVFGLLMSLNMLIETTGGFDYTIAACEGWMRDAGFASTQHKALAGPHSMVVG
                     IK"
     gene            2693..>3294
                     /locus_tag="VIR04_07130"
     CDS             2693..>3294
                     /locus_tag="VIR04_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY9460554.1"
                     /translation="MQAFIAAALRKHPLFANGTRIELARLLGGTDHREIAVDTEIFQA
                     GADADYYYWILSGSVELSTEHNTWRMGPGEGFGAEAFAEDCQYLLSARVATPLTALRI
                     AHAVIQRFAAAHPHVKSSALTSLAARLGNMPLPKTRFAATGRSADIPMKQRIGWVATL
                     ILPPAAYLTAADNGLPQYSAIYLGLLTMTAVMWLFAVVDE"
BASE COUNT          791 a          943 c          879 g          681 t
ORIGIN      
        1 gccgcaattg cgttccatgt acaaccgcgc cactggcaag ccgcagattc gcttttatga
       61 aggcgaacaa ggaatcataa cggcgctttg ggacaccttg acggtgacct ccgagcccaa
      121 ggtattgcgt ggcatccttt ccatggatga actaatgcaa acgcctggaa tggcggaaat
      181 ggagcgattc atcgcaaacc gcgtccaaag agacatttgg ctgaaagtaa tccgctccga
      241 acagaaagac gtcgacgcga tctggccctc gaatgtcgaa aaattgcgtg atttgcgcta
      301 cgcgccaccc gaaatgctgc tgtcgatgac tacctttatt tatgaccatc gcgtttgtct
      361 gatctcgtca aaaaaagaaa actacggcct gatcattgaa agcgaagaat tttcgaccat
      421 gcaaaccgtg ctgttcgacg ggatttgggg attaagcaaa gcgcctgact agccccgcgc
      481 cgcgcaggcg aaactccaaa ttgcacaggc cgccggcaaa ctgaagctag tgcggctccg
      541 ccacaaagcg cgtgagactt acgtccacag tgactgataa cgccgtcagt gcggcggcct
      601 tcgcgcgccc ctcggcgctg gctcgataaa gatggggcgt catcatcagc aaatcggcaa
      661 tttgttcttt gctgtcaaga ctgagggaat atcgaatgtg ttcaccgggc aaatcggcga
      721 aaccctccgg tgtctgagcg cgaacgccgt cctttggctt taacgtgggg taaatgattt
      781 cgcgcaattc cctcaaatga tctgatccgg catccacctg caacaactgc ccccccggtc
      841 tgagtacacg cgcaaactcc gcgtagacgg ggaaaccgaa catgcatagc acccggtcaa
      901 gcgaacgcgc ccgcactggc aagttggcat tgctgcctac aacccaactg gaccgcttgt
      961 cgtgcttggc tgcggacaaa attgcccatt tggagatatc cagcccaagc agagccaaag
     1021 tctgttcgtc gccggcttcg gccgccaact gatgcaggta atagccatcg ccgcatccgg
     1081 cgtccaggca attgaccgtt atcccggacg gcaaatctgc aagcaccgcc ttgcctacca
     1141 tagcggcaat cggctggtaa aaaccggcac tcaggaaacg ccggcgtgcg gcaaccatct
     1201 gtttgctgtc gcccgggtcg cgcgaatgct ttttctgaac tggcagcaaa tgaacgtagc
     1261 cctgtctggc aacgtcaaaa ctatggcccg atgcgcaacc ccatgtgtcg ccagttcggc
     1321 gcaacgcgac gccatcaagc ggacagacca aatccagaaa cggaacgatc atggtctgcg
     1381 catgcgagag tccgctttat caatacaggt tgccactttg ctgatgcctc atcatgtcct
     1441 gaagcgtttc aaaacgccgc caaaaaacaa cttgccaagg gttgccgggc atctatacta
     1501 actgattcca ggctgctacc cccaagggcc gccacgcagg tcgaatgcca aaacgagagc
     1561 cgacgatgaa tgaatatgct tcagcagaga aaatccttca acttgggacg gcgttctggg
     1621 cttccaaaac actgctgtcg gccgtggagt tgaagctgtt ttccgaattg gcaaaaaagc
     1681 cgctcgatca tggcgcgcta gcggcaaaat tggggctggg cagccggggc acgctggatt
     1741 ttttcgatgc cctggttgcc cttggaatgc tcgaaattca taacggaaag tactgcaaca
     1801 ctcctgaagc agcactcttt cttgatgcaa gcaaagaaac atacatcggc ggaattctgg
     1861 agatggccaa tgatcgcctc tatcctttct ggggacgcct gaccgaagcg ctaaaaaccg
     1921 ggcaaccgca aaacgaagcc aagagtggtg aaaacacgtt tgcaacgcta tatgcggacc
     1981 cggcaaggct caagcaattt ctccaggcaa tgaccggcgt aagtttggcc aatgccacgg
     2041 cactcgctca ggaatttccc tggtcgaact atcaatcctt cgcggacatt ggctgtgcac
     2101 agggcgccgt gccagtgcaa gtggcaaaat tccatcctca cctgagcgtt gccggcttcg
     2161 atttgccgtc ggtgcggcct atcttcgaag aatatgtgca gtcacatgga ctgaccgcac
     2221 gggttcgctt catttcgggc gacttcttca atgatccctt gccgcatgtt gatgtcatcg
     2281 tgatgggcca tgtacttcat gactgggacc ttgatcaaaa acgcacgttg ctcaacaagg
     2341 cgtacgcgtc tttgcccaag ggaggcgcac tgatagtcta tgacgccatg atcgataacg
     2401 aacgcaagaa aaatgtcttc ggcctgctca tgagcctgaa catgctgatt gaaacaacgg
     2461 gcggattcga ctacaccata gccgcatgcg aaggctggat gcgcgacgcc ggcttcgcat
     2521 cgacccagca caaagcgctg gccggcccgc attcgatggt ggtgggaatc aaatagcgtg
     2581 cgcaattggg agcaatatca ctcgcccaag aacctcgcta cggctgtatc taaacggtca
     2641 gtatttgttt aagatatgca cttgctcgaa tccaacccgg acgcggatac gcatgcaggc
     2701 tttcatcgcc gccgctttac gcaaacatcc cttatttgcc aacggcacac ggattgagct
     2761 ggcgcgccta ttggggggga ctgaccaccg tgaaatcgcg gtggatacgg aaatttttca
     2821 ggcgggcgcc gacgctgatt attactattg gatcctgtcc ggaagcgtcg aactctccac
     2881 tgaacataac acctggcgca tggggcccgg ggaaggcttc ggcgcggagg cattcgcgga
     2941 ggattgccaa tacctgctta gcgcccgggt cgccacgccg ctgaccgcgc tacgtatcgc
     3001 acatgccgta atacagagat tcgcggcggc gcatccccac gtaaaatcca gcgccttgac
     3061 cagcctggcg gcacggctag gcaacatgcc cctaccgaaa acccggtttg ccgcaaccgg
     3121 acgcagcgcc gacattccga tgaaacagcg catcggttgg gtggccacat tgatactgcc
     3181 acccgcagct tatctgaccg cggccgataa tgggttgccg caatatagcg ccatctatct
     3241 gggtctgctc acgatgacag cggtaatgtg gttgtttgcc gtcgtcgatg aatt
//
LOCUS       DATCXU010000319         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_896637,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000319 DATCXU010000000
VERSION     DATCXU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896637"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VIR04_07135"
     CDS             <1..320
                     /locus_tag="VIR04_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460555.1"
                     /translation="DSFQPPALYLRMMPADIDNLFFIGLFQPLGCIWNLADHQARIAA
                     LQITGKLARPADLGARIEQEIRHPHWRYQRSVRHAIEVDFYEFRKALLAEVAKARLER
                     PSA"
     gene            complement(478..1641)
                     /gene="lptF"
                     /locus_tag="VIR04_07140"
     CDS             complement(478..1641)
                     /gene="lptF"
                     /locus_tag="VIR04_07140"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY9460556.1"
                     /translation="MGSIGRYVFRTTFGVFLVVLISLTAVIWVTQALRDIDLITSQGQ
                     TVLTFVGITSLIIPFLIMTIAPIALVIAVTHVLNKLSTDSEIIVMNAAGMSPWLLFRA
                     FLSVAFVVSLIVCVIGTYFGPKGLRMLRDWVTKVHAQVVTTVVQPGRFTTIQSGVTFN
                     IRERRNNGQLFGVFLDDRRNPNERITIIAEIGELLEGDNGTFLIFQNGVLQRQQTKES
                     DPSMVVFDSYAFNLSQFAGGPQAVNYSIRERYLWQLIFADPNDRSYIEQPNRFRAELH
                     DRLLGPLYPLAFVVIALAYLIAPRTTRQSRMLSIVYVIGWVALLRLVGVVSNVLGVTT
                     PWMLSLPYVAITIVLTFGLFVIHTGAVLETPAFLVNWFAAIGQRNRGRIAEKS"
     gene            complement(1743..3344)
                     /locus_tag="VIR04_07145"
     CDS             complement(1743..3344)
                     /locus_tag="VIR04_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY9460557.1"
                     /translation="MNIDSTKFLRRAVPILVALTLLARAIGMALVPLMDTTEARYGEI
                     ARKMAELNDWVTPWFDYGVPYWGKPPLSFWLTAISFKVFGVNEFAGRLPHWIASLIII
                     ALVWALSGRRDRDAAMPAVAVISASALFFVSAGAVMTDIELTVGTTLAMTGFWLALDK
                     PKTGSSGFEWFAGLLFFSGLAIGLLAKGPVALVLAGTPLFLWTTYNRRWLDVWRRLPW
                     ILGVLGTLVITLPWYWIAEQRTPGFLAYFLIGEHWHRFVTPSWQGDRYGHAHTFPIGT
                     IWAFAFIGTLPWSLLLPIAAWRWRKRKDEASAATMPVVNTPALRSDEHAWQSYLLAWA
                     FTPLLFFTAARNITMAYVLPGLPAAAILTGSWLAQQRRHGRAVNQLLSVGMLITLVLV
                     CRLAIYNGEPFHMERRSLKALVTAYDQARARGGSFSATVLPKGQSPQSNVPLVFVGPR
                     PFSAQFYSHGHAIQVDSIDQCWRRIGAGPAYVAIPSRYSDALITGAAAHQGTDSNKTE
                     AAKRSYTVSRVYRHGNFDLFYVAAH"
BASE COUNT          848 a         1056 c         1029 g          689 t
ORIGIN      
        1 gggacagctt tcagccgccg gcgctctatc tgcgaatgat gccggccgat atcgacaatc
       61 ttttcttcat tgggctattt caaccgctcg gctgcatctg gaatctcgcc gatcaccaag
      121 cgcggattgc ggcccttcag atcacgggca agctggcgcg ccccgccgat cttggcgcgc
      181 gcatcgaaca ggaaatcagg catccccatt ggcggtacca acgatcggtg cgccacgcca
      241 ttgaggttga cttttacgag ttccgcaagg cgcttctggc ggaggtcgcc aaggctcgac
      301 tggaacgacc gagcgcctaa agaaatcacg cgtccctgct tcagccagtc tccatgacat
      361 tgaacacgcg ttcacgacac ccagtggtgc tccagaaacc ctccaatggc ggcacttggg
      421 tccaaagcac tcggattttg ccattccttc ggcccgtcgg cgaataggct gtggccatca
      481 tgatttttct gcaatccgcc cgcgattgcg ttgaccgatg gccgcaaacc agttgacgag
      541 aaatgcgggc gtttccagga ccgcgccggt gtggatcacg aacaagccga atgtgagaac
      601 gattgtgatc gcaacatatg gcagcgacag catccatggc gtcgtaacgc cgaggacgtt
      661 tgaaacaact ccgaccaggc gaagcaacgc tacccagcct atgacataga cgatcgagag
      721 catgcggctc tgtcgggtgg tgcgaggcgc gataaggtaa gcgagcgcga tcaccacgaa
      781 ggcaagcggg tagagcggtc caagcaaacg atcgtgcaat tcagcgcgga atcggttcgg
      841 ctgctcaata taggaccgat cattgggatc ggcgaatatc agttgccaca ggtagcgctc
      901 acggatcgaa tagttgactg cttggggacc gccagcaaat tgcgaaaggt taaaagcgta
      961 gctgtcgaag actaccatcg acgggtcgct ttctttagtc tgttggcgtt gcaagactcc
     1021 attctgaaaa atcagaaacg ttccgttgtc gccttccagc aactcgccga tctcggcaat
     1081 tatggtgata cgttcgtttg gattgcgccg gtcgtcgaga aaaactccga acaactggcc
     1141 attattccgg cgttcccgaa tattgaacgt tactccgctt tggatcgtgg tgaagcggcc
     1201 cggctgaacg acggtcgtga ctacttgcgc gtgcaccttt gtgacccagt cacgcaacat
     1261 tcgtagcccc ttcggtccaa aatatgtgcc gatcacacac acaattagcg agacgacaaa
     1321 tgcaaccgat aagaaggcac ggaacaaaag ccacggcgac atgccggccg cgttcatgac
     1381 gatgatttcg gaatcggtcg ataatttatt gagtacatgc gtcaccgcaa tgaccagcgc
     1441 gatcggcgcg attgtcatga tcagaaacgg aataatcagg ctcgtgatgc cgacgaatgt
     1501 cagaacggtc tgcccttgcg aagttatcag atcgatatcg cgcagtgcct gcgtaaccca
     1561 aattacggcc gttaggctga tgagcacgac caagaacaca ccgaatgtgg tgcggaaaac
     1621 atatctgcca atggacccca tccgaactgc tccgcagcgg caagctgccg gtctccgcgc
     1681 ggcctctctc caccgacacg tgctccttta tcttagaata tcccgcacag acttcagagt
     1741 gctcagtgcg cggcgacgta aaacagatcg aagttaccgt ggcgatagac gcgactcacg
     1801 gtgtaagagc gtttggcggc ttcggttttg ttcgaatccg tcccttgatg cgccgccgca
     1861 ccggtgatga gtgcgtcaga ataacgagac ggaatggcca cataggcggg accagcacca
     1921 attcgccgcc aacattgatc gatactgtct acctggatgg catgaccatg actatagaac
     1981 tgggctgaga acggacgggg tccgacaaac accagtggca cattggattg tggggattgg
     2041 ccttttggca aaacggttgc ggaaaaagaa ccgcccctcg ctctggcctg gtcgtaggcc
     2101 gtcaccagcg ccttgagcga cctgcgctcc atatggaaag gttcaccgtt gtagatcgcg
     2161 aggcggcaca ccaacaccaa cgtgatcagc atgccgacgc tgagcagctg gttgaccgcg
     2221 cggccatgtc gtcgctgctg cgccagccag ctgccggtca ggatggcggc cgcgggtagc
     2281 cccggcaaca cgtaggccat ggtaatatta cgcgcggcgg tgaagaacag cagcggcgtg
     2341 aaggcccagg ccagcagata gctttgccag gcatgttcgt cagaccgcaa tgcaggcgta
     2401 tttacaaccg gcatcgttgc agccgatgcc tcgtctttgc gcttgcgcca gcgccacgcg
     2461 gcgatcggca gcagcaatga ccagggcaac gtgccgatga aggcaaacgc ccagatggta
     2521 ccgatcggaa acgtgtgcgc gtgtccgtag cggtcaccct gccagctggg cgtaacgaaa
     2581 cggtgccagt gttcgccgat caggaaatac gcaagaaagc ccggcgtgcg ttgctcggcg
     2641 atccagtacc atggcaacgt gatcacgagt gtccccaaca caccaagaat ccacggcagg
     2701 cggcgccaga cgtcgagcca gcggcggttg tatgtcgtcc aaaggaagag tggcgtcccc
     2761 gccagcacca aggccacggg tcccttcgcg agcaagccga tagcgagacc gctgaagaac
     2821 agcaggcctg cgaaccactc gaagccagat gagccagtct tcggtttatc cagtgcaagc
     2881 caaaaaccgg tcatcgccag cgtggtaccg acagtcaatt cgatgtcggt catcacagcg
     2941 ccggccgaga cgaagaaaag ggcgctggcc gagatcaccg ccaccgccgg catagcggca
     3001 tcgcggtcgc ggcgccctga cagtgcccaa accagagcta tgataatcag cgaagcaatc
     3061 caatggggca atcgcccagc gaattcattt actccgaata ccttgaaact gattgcagtc
     3121 agccagaatg aaagtggtgg ctttccccag tacggcacgc cgtagtcgaa ccacggcgtg
     3181 acccagtcgt tcaactccgc catttttcgg gcaatctcgc cgtagcgggc ctccgtggtg
     3241 tccattagcg ggactagagc catgccaatc gcacgggcca gcagcgtcaa tgcaaccaag
     3301 atcggaacgg cgcggcgaag gaatttcgtg gaatcgatgt tcattcaggg aatcttgcgt
     3361 gcgacgttca ctattaccac aggttcacag cgatcaacgg tgtggccgcg ctggctagcg
     3421 gacggccctt gaacaactgg acagcgccat ctctccaccc cgctcgtgta ctagttatca
     3481 tggcgccaaa tatccgggca gaaacctgct tcagcgccaa aggcggtgaa gccaaagcca
     3541 ttgatctgga tgctctcgca cccatccctc gacgatcgac ataatcgcct gcatggtgcc
     3601 ggcgacgtcg atttggccat cg
//
LOCUS       DATCXU010000320         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1045472,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000320 DATCXU010000000
VERSION     DATCXU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045472"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIR04_07150"
     CDS             <1..479
                     /locus_tag="VIR04_07150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9460558.1"
                     /translation="PGILLEELSFEQWQRVINVNLTGVFLCIQQAFRVMKAQTPQGGR
                     IINNGSISAHTPRPNSIPYTASKHAVTGMTKAASLDGRKYNIAVGQIDIGNAETEMAA
                     RMAKGVMQANGEIAAEPMMDVNIVGQSVLYMASLPLEANVMFHTVMATRMPFAGRG"
     gene            complement(543..619)
                     /locus_tag="VIR04_07155"
     tRNA            complement(543..619)
                     /locus_tag="VIR04_07155"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Pro,seq:tgg)
     gene            complement(675..2579)
                     /locus_tag="VIR04_07160"
     CDS             complement(675..2579)
                     /locus_tag="VIR04_07160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY9460559.1"
                     /translation="MQELTLTFIVSSLCLLTLSRCLLIAWQWRRVKQAGGPLPILLGG
                     LRVDANQIAALSIIPALLTPWIGHWPIAATLTSWWFQLAWLLLALLEVSTPQFIHEYD
                     TRPNRLYVQYLKHPKEVFGMLWRGYKGVIAGAGITMAGFAWLGYRLFGHALPDAPMPW
                     WERPLFTLASAAIAVLAIRGSLGHRPMNPSSVAYCGDSMLNTLPLNSLYNVAYAIYSM
                     KNERSAADVYGDMPSDEMHQIVRACAGIVHNEDSDIPTLHVQLPIQERKQPLNLVIIV
                     EESLGAQFSGALGGANLTPCLDQLASEAWNFTHAYATGTRSVRGLEALVAGFPPTVSD
                     AVLRLPGAQHNFFTMAQLLKQHNYRARFIYGGEAHFDNMKGFFLGNGFDDLYDHPSFE
                     NPNFVGTWGASDEDMFNQLDHLLSRPPEHPTFTLAFSVTNHSPWEYPSGRIEPVGDPA
                     TVENSVRYADWAIGQFFNKAKRSGYWENTVFLIAADHDSRVFGANLVPVRHFHIPSLI
                     VGADVAARQDDRLISQIDLPPTLLSIMGLRTEHPMIGHDLTQGGGGRAMMQYGENYGY
                     LKNDTLLVLEPHRQPSQYHYQAPETYHPMAIDPHLTREALSHVLWPAWVYQHQRYTLP
                     QLRAGAQANI"
     gene            2691..3872
                     /locus_tag="VIR04_07165"
     CDS             2691..3872
                     /locus_tag="VIR04_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460560.1"
                     /translation="MGIAILGVAATSAWAQDTIKIGELNSYKTQPAFLGPYKKGMELA
                     LEQVNAAGGVNGKQLQLITRDDNSNPGDAVRAAQELLTRDKADVLAGAFLSNIGLAVT
                     DFAKHQKVFYLASEPLTDKIVWADGNRYTFRLRNSTYMQVAMLIPAAVKMNKKRWAIV
                     YPNYEYGQSAVATFKALLKAAQPDVEFVAEQATPLGKIDAGSVVQALSDAKPQALFNV
                     LFASDLTKFVRAGTQRNFFGGLPVVSMLTGEPEYLDALGGETPVGWVVTGYPWYAINT
                     PEHTKFLHAYQAKFNDYPRLGSVVGYNTIMSLAAGMEKAGTTKTEALIAAFKGLKVNT
                     PFGPIVYRSIDHQSTMGAYVGTLAVKDGKGIMTDFKYVDGSTVQPPDDQVKKWRPAGA
                     D"
     gene            3913..>4439
                     /locus_tag="VIR04_07170"
     CDS             3913..>4439
                     /locus_tag="VIR04_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460561.1"
                     /translation="MDLTGIAVQFLNGLAQASSLFLVAVGLSLIFGVTRIVNFAHGSL
                     YMLGIYIAYSAVGHWGHDPFGFWLSLIAASLAVGVLGALIEIVVLRRIYRAPELFQLL
                     ATFGLVLVINDAALWAWGPDDLLGPLAPGLSGAIPVFGRYIPVYDLVLIVVGPALLVS
                     LWLLLTRTRWGALIRA"
BASE COUNT          954 a         1159 c         1334 g          992 t
ORIGIN      
        1 cgccgggcat tttgctggag gagctgtcct ttgagcaatg gcagcgcgtg atcaacgtta
       61 atttgaccgg cgtattcctt tgcatccagc aggcgtttcg ggtgatgaag gcacaaacgc
      121 cccagggagg ccgcatcatc aataacggct ccatctcggc gcatacgccg cggccgaact
      181 ccattcccta caccgcaagc aagcatgccg taacgggcat gaccaaagcg gcgtcgctgg
      241 acggccgcaa atacaatatt gccgtgggcc aaatcgatat aggcaacgcc gagaccgaaa
      301 tggcggcacg catggcaaaa ggcgtgatgc aggccaacgg cgaaatcgcg gccgaaccga
      361 tgatggacgt aaacattgtc ggccaatcgg tgctttacat ggccagtctg ccgcttgagg
      421 ccaacgtaat gttccacaca gtcatggcga cccgcatgcc gttcgcgggc agggggtaat
      481 ccggggttat cgaaaatact taagggctta gcgctgtttt agctctaagc ccttgatttt
      541 attggtcggg gcgaaaggat tcgaaccttc gaccctctgg tcccaaacca gatgcgctac
      601 caggctgcgc tacgccccga caagagcgcg attatgacag atcgcacgat gtttgtcttg
      661 tgcgggcgct gtcgtcatat gttagcctgc gcccccgcac gcagttgcgg cagggtatag
      721 cgctggtgct ggtataccca ggccggccac agtacgtgag acagcgcttc gcgggtcaga
      781 tgcggatcga tcgccattgg gtgatacgtt tcaggcgcct gataatggta ttggcttggc
      841 tgcctgtgcg gctcaagcac cagcagcgtg tcatttttca ggtagccgta gttttcacca
      901 tattgcatca tggcgcgtcc gccgccacct tgcgtcagat cgtgcccgat catcgggtgt
      961 tcggtgcgca ggcccataat cgacaaaaga gtggggggca aatcaatctg actgatgagt
     1021 cgatcgtctt gccgcgcggc aacatcggcc ccgacgatca aagacggaat atgaaagtgc
     1081 cgtacaggca ccaggttggc gccgaagacg cgcgagtcgt ggtccgccgc gatcaggaaa
     1141 acggtgtttt cccaatagcc ggatcgcttc gccttattga aaaattgacc tatggcccag
     1201 tcggcgtaac gcactgagtt ttcgacggtt gcgggatcgc ctaccggttc gatgcggccg
     1261 ctggggtatt cccatggcga atggttggtt accgaaaacg ccaaggtgaa ggtggggtgt
     1321 tcgggcggcc ggcttaacaa gtggtccagt tgattgaaca tgtcttcatc cgaagcgccc
     1381 caggtcccca caaagttcgg attttcgaag ctgggatggt cgtacaggtc atcgaagcca
     1441 ttgcccagaa agaagccttt catgttgtcg aagtgcgctt ctccgccgta gatgaatcgt
     1501 gcccggtagt tgtgctgctt taatagttgc gccatggtga agaaattgtg ttgtgcgccc
     1561 ggcaggcgca ggacagcatc cgacacggtg ggtggaaagc ccgcgacaag cgcttcaagg
     1621 cctcgcacag aacgggtccc tgtggcataa gcgtgcgtga aattccaggc ctcgctggca
     1681 agttgatcca ggcaaggcgt caggttggcg ccgcccaaag cgccgctgaa ctgagcgccc
     1741 aggctttctt caacaatgat gacgaggttc agcggctgtt tccgttcttg aatcggcaac
     1801 tgaacgtgca gagtcgggat atcggagtct tcgttgtgga cgatgcccgc gcaagctctg
     1861 acgatctggt gcatctcgtc gctgggcatg tcgccataaa catcggcggc ggatcgttcg
     1921 tttttcatgc tatatatagc gtacgcgacg ttatacagag aattcagcgg cagggtgttg
     1981 agcatgctgt cgccgcagta ggcgacggaa gatgggttca ttggacgatg gcccaatgag
     2041 ccgcgaatcg caaggacagc gatggcggcg ctggcaagcg taaacagcgg gcgctcccac
     2101 catggcatcg gagcgtcggg caatgcgtgg ccaaatagcc tgtagcccag ccacgcgaag
     2161 ccggccatcg taatgccggc gcctgcaatg acgcccttgt atcccctcca gagcatgccg
     2221 aagacttctt ttggatgttt caggtattga acataaagac ggttgggccg ggtgtcgtat
     2281 tcgtgaatga actgcggtgt tgaaacttca agcagagcca gcagcagcca tgccaattga
     2341 aaccaccagg aagtcagggt agccgcgatt ggccagtggc cgatccaggg cgtcagcaac
     2401 gccggaataa tggacagggc ggcaatctga ttggcgtcca cgcgcaagcc gcccagcaaa
     2461 atgggaagcg ggccgcccgc ctgtttgacg cgtcgccatt gccacgcgat cagcaggcat
     2521 cggcttaagg tcagtagaca caggctggaa acgataaagg taagagtcag ttcttgcatg
     2581 cagtacagta aaggcttttc gccatcgaca ttacgtataa tttacagttt gattgacggc
     2641 gtcaatcatc aaaatgggaa tcaaatatga tccggttgtt cataaaggta atgggcatcg
     2701 ccatcttggg ggttgccgcc acgtcggcat gggctcaaga taccatcaaa ataggcgaac
     2761 tcaatagtta caaaacgcag ccggcctttc ttggtcctta taaaaaaggc atggagctgg
     2821 cgctggaaca agtcaatgcg gccggcggag ttaatggcaa gcagcttcaa ttgattacac
     2881 gcgacgataa ttctaatccg ggcgatgcgg tgcgcgcggc ccaggaattg ttaacgcgcg
     2941 acaaggcaga tgtcctggcc ggggcttttc tgtcgaatat tggcttggcc gtgacagatt
     3001 ttgccaagca tcagaaagtg ttttatctgg ccagtgaacc tctgaccgat aaaattgtat
     3061 gggccgatgg caatcgctat accttcagac tgcgcaattc gacctacatg caagtcgcca
     3121 tgctgattcc cgccgccgta aaaatgaaca agaagcgctg ggcgattgtg tatcccaact
     3181 acgaatacgg gcaatccgcc gttgcaacat tcaaggcatt gttgaaggcg gcacagcccg
     3241 atgtcgaatt cgtggctgaa caagccaccc ctttgggcaa gattgacgca ggcagtgtgg
     3301 tgcaagcgct gtccgatgcc aaaccgcaag cgttgttcaa tgttttgttc gccagcgatc
     3361 tgaccaagtt tgtacgcgcc ggcactcaac gtaatttttt tggcggcttg cctgtggtca
     3421 gcatgctgac cggcgagcct gaataccttg atgccctggg cggcgaaacc ccagtgggct
     3481 gggtggtgac cggctacccg tggtatgcca taaacacgcc cgaacatacc aagttcctgc
     3541 atgcgtatca ggcgaagttc aacgactatc cgcgtttggg ttcggtagtg ggctacaaca
     3601 ccatcatgtc cctggctgcc ggcatggaaa aagcgggcac gaccaaaacg gaagcactta
     3661 ttgcggcgtt caagggactc aaggtgaata cgcccttcgg gccgattgtc tatcgttcca
     3721 ttgatcatca gtcaaccatg ggtgcttacg taggtactct ggctgtcaag gatggcaagg
     3781 gaataatgac cgacttcaaa tacgttgatg gctccacggt tcaaccgcct gacgatcaag
     3841 tgaagaaatg gcgccccgcg ggggctgatt aacggcagca acgcgttgta gcgcgaggca
     3901 aggcgcaata acatggacct taccggtatt gccgttcagt ttctgaatgg gctggcgcag
     3961 gcttcgtcgc tgtttcttgt ggcggtgggt ctgtcgctta ttttcggtgt cacgcgcatt
     4021 gttaattttg cgcatggctc gctgtatatg ctgggcattt acattgccta ttcggccgtt
     4081 ggccattggg gtcatgatcc gtttggtttt tggcttagtc tgatcgcggc gtcgctggcg
     4141 gttggcgtgt tgggcgccct gatcgagatc gttgtgctgc gccgcatata ccgggcgccc
     4201 gagctatttc agttgctggc cacgtttggg ctggtgctgg tgataaatga tgccgcgctg
     4261 tgggcgtggg ggccggacga tttgctgggc cctctggctc cgggactgag cggcgccatt
     4321 ccggttttcg gccgttacat acccgtctat gacctggtgt tgattgttgt cgggccggcg
     4381 ctgctggtga gcctgtggct gttattgacg cgcacccgtt ggggcgcgct gatacgtgc
//
LOCUS       DATCXU010000321         7181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_171770,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000321 DATCXU010000000
VERSION     DATCXU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171770"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VIR04_07175"
     CDS             complement(<1..672)
                     /locus_tag="VIR04_07175"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003553.1"
                     /note="catalyzes the transfer of a segment of a
                     1,4-alpha-D-glucan chain to a primary hydroxy group in a
                     similar glucan chain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEY9460562.1"
                     /translation="MNKRISSSHRARGPGSATTLAANAVRAIDPAKIDALAAGRHGDP
                     FSILGPHQMDQHRILRAFLPGACGVQAIGSDPATACQLFELRDGFFIGVLSDSIEDYR
                     LHIRWPDSEQETEDPYSFGPLLGELDMYLIAEGRHWHLADCLGAHVMSVNGVSGACFA
                     VWAPNAQRVSVVGDFNSWDGRRHPMRLRRECGVWEIFIPRVRAGARYKFELLAGDGRL
                     LALKAD"
     gene            complement(656..4123)
                     /gene="treS"
                     /locus_tag="VIR04_07180"
     CDS             complement(656..4123)
                     /gene="treS"
                     /locus_tag="VIR04_07180"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY9460563.1"
                     /translation="MATPRPSHVAASNEPLWYKDAVIYQLHVKSFFDANNDGVGDFAG
                     LTAKLDYIVELGVTTVWLLPFYPSPRLDDGYDIAEYRGVHSDYGVLSDVRKFIRLAHR
                     RGLHIITELVINHTSDQHVWFQRARHARAGSAARNFYVWSDNDQAYAGTRIIFCDTEK
                     SNWTWDPVAGAYFWHRFYSHQPDLNYDNPRVLREMIGVMRFWLDMGVDGLRLDAVPYL
                     VEREGTSNENLPETHIILRQIRAVMDADYPDRLLLAEANQWPEDAQEYFGKGDECHMS
                     FHFPLMPRMYMAIAQADRFPITDILRQTPEIPENCQWAIFLRNHDELTLEMVTGSERD
                     YLWEVYAADRRARINLGIRRRLAPLLERDRRRIELMNSLLFSMPGTPVMYYGDELGMG
                     DNIHLGDRDGVRTPMQWTPDRNGGFSRADPERLSLPVLMGPLYGYEAVNVEAQQRDPH
                     SLLNWTRRMLAKRQQTHAFGRGTLRFLYPGNRKILAYLREYDGVALLCVANLSNASQP
                     VELDLAFMAGRVPVELLGGTPFPTIGELPYLLTLPPYGFYWFELSGNAAPPVWHATMP
                     EQMPEYVTLVVRRRAGAAYQLVEGSRKTLCSEVLPLYLARQRWFARGQRIRAVQISYG
                     MPMPGQPDLFMAEFEVEFADGAARYFLPLALLWGELISGPPQQYGLARVRRGSELGLV
                     TDAFSVPDFALTLLDNLRAHREISVETSDGRCLLRFQGEKGLNTLQFPQDLTVDWFHG
                     EQSNSSMTLGHTAILKLMRHVVPGIHPEAEMTRHLRLAGYTNSAELLGELIRVNPDGT
                     PHTLALLHAQIGNQGDAWAWTQDYLKRTLENTGLTEEGQPDYEEELHGYAAVASAIGR
                     RLAQLHATLAQPSDDPAFAPEAASIADAKHWVKDIRTMLGRAITAVSARKAGLSDQGR
                     TQIDELKSSHKALSKAIDKRAQALAGTLRTRIHGDFHLGQVLIAQNDVYLIDFEGEPV
                     RPLDQRRGKASPWRDVAGLLRSFEYAAAAFDKSEQATDVVLKPVVSPPEGEAQTRSEG
                     EPLAQPVKRSPQQNRSTLLGQFRDIARESFLNAYLATDQDAIAAPDDNPGRPTAAPSK
                     IIAFQASGGAALLQMALLEKAAYEVCYEAAHRPDWLSIPLTGLAQAARRMLAEPPPSQ
                     ENPHNEQKD"
     gene            complement(4180..>7181)
                     /locus_tag="VIR04_07185"
     CDS             complement(4180..>7181)
                     /locus_tag="VIR04_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY9460564.1"
                     /translation="GGMASVCAQHGLDLMTDLVLDKTVGEEPARVRGNDGLARIEFIE
                     AWSRRLGGWVRAGVSGFRCRAPAELSRGEWRRLIDNVRSLQPHCHFMAWTPGLGAGQI
                     DRLKGAGFDATFSSLPWWDYRATWLIDEYVRLGAIAPVFSPVTDPDTVVDPADFDLAQ
                     AGNDARRKIWTAAIAGNGVLVTTSLEKIAGSLTLAQLNKWLQDRPNTTGRLRVLSGAL
                     SPATVLFRSGAGLLINPDGQVDATKKWADVRARLPHDNGMIQVMPDPDGAAGVPCSDA
                     AELKPGGCLLFLTFSDQCVTATATTSEDKRKAVVAALRAARITIDGVIPEVDKGRFPA
                     KTQIGETVVVQADIFMDGHERILASMLWRAADEVSWRETFMTMLENDRWEARFSPTRI
                     GKHYFTIRAWHDRWGSYREQLAKKVAAGQDVSLDVEEGRALLLNAVQPRKTSDRPMDD
                     SSQYVQSLTRILDDVGMPRQTPRLKARRAAAARSECAVVAGLPVPPARPEQVEILLSE
                     TVAAAMRETDIRRFEATCERPFEIGVERREASFASWYELFPRSCGSKPGVHGTFRDVM
                     KRLPAIRDMGFDVLYFPPIHPIGLSNRKGRNNSLKASPEDPGSPYAIGSVLGGHTSVH
                     PQLGTLQDFQALVERARQFELEVALDFAIQCSPDHPWLAQHPEWFSWRADGSLRYAEN
                     PPKRYEDIVSPSFYSSLASASRQMALWRELRDVVLFWIEHGIHIFRVDNPHTKPLPFW
                     QWLISDVQGTHPDTIFLSEAFTRPKMMYRLAKIGFSQSYTYFTWRNTKQELIDYLTEL
                     NSPSIASFFRPHFFVNTPDINPYFLQTSGRAGFLIRAALAATTSGLWGMYNGFELCEA
                     RALPGKEEYLDSEKYELRSWDWNRPGNIVPEITKLNQIRRTNPALQSHLGIRFHHVDD
                     DRILFFSKSTPQRDSIVLVAISLDPYAVRFGTLELPLWQWSLPDNATVLLEDLFDDRR
                     FALSGKHHRVELTPQRPFLLWKVLPPI"
BASE COUNT         1565 a         2158 c         1992 g         1466 t
ORIGIN      
        1 gtcggccttt agcgcaagca accggccgtc tccggccaat agttcgaatt tataacgggc
       61 ccccgcgcgt acgcgcggaa taaatatttc ccatactccg cattcgcgcc gcaaacgcat
      121 cgggtgacgc cgtccgtccc acgaattgaa atcccccacc accgacacgc gttgcgcgtt
      181 aggcgcccac accgcgaaac atgcgcccga tacgccattc acgctcataa cgtgggcgcc
      241 aagacaatct gccaaatgcc aatgacgccc ttccgcaatc aggtacatgt ccagctcgcc
      301 cagcaatggg ccaaagctat aaggatcctc ggtttcctgc tcggaatcgg gccagcgtat
      361 gtgcaaacga tagtcctcaa tgctgtcgct caatactccg ataaaaaaac catcgcgcaa
      421 ttcaaacagt tgacacgccg ttgcagggtc ggagccaatc gcctgaacgc cgcatgcgcc
      481 cggcaaaaat gcgcggagga tccggtgctg atccatttgg tgcggcccca atatcgaaaa
      541 gggatcgcca tggcggcctg ccgccagggc atcgatcttc gccgggtcga tggcccttac
      601 ggcgttggcg gccagtgttg tcgccgagcc tggcccgcgc gccctgtggc tcgaactaat
      661 ccttttgttc attgtgcgga ttttcctgag atggaggtgg ttctgccagc atgcgccggg
      721 ccgcttgagc cagaccagtc agcggaatgg acagccagtc cgggcgatgc gctgcttcgt
      781 agcacacttc gtaagccgcc ttctccagca aagccatttg caacagcgca gcgccgccgc
      841 ttgcctgaaa tgcgatgatc ttcgagggcg cggcagtcgg gcggcccgga ttgtcatcgg
      901 gcgccgctat ggcatcttga tctgtggcaa gataggcatt caaaaacgat tcgcgcgcaa
      961 tgtcgcgaaa ttgaccgagc agagtgctcc ggttttgttg cggcgagcgc ttcaccggct
     1021 gagccagcgg ctcgccttcc gatcgggttt gagcctcgcc ttcgggtggc gagacaaccg
     1081 gcttcaacac gacgtccgta gcctgttctg atttgtcaaa ggcggccgcc gcatactcga
     1141 aagaacgcaa cagcccggcc acgtctcgcc agggactggc tttgccgcga cgttggtcaa
     1201 gaggccgcac gggttctccc tcgaaatcaa tcagatatac gtcattttgg gcgatcagca
     1261 cttggccaag atgaaaatcg ccgtgaatgc gagtgcgaag ggtgccagcc agcgcctgcg
     1321 cccgcttgtc gattgccttt gacagcgcct tgtgcgagct ttttagttcg tcaatttgcg
     1381 tgcgtccctg atcggaaaga ccggctttgc gggccgaaac cgcggtgatg gcgcgtccca
     1441 acatggtccg tatgtctttc acccaatgtt ttgcgtccgc gatgctcgcc gcctcgggag
     1501 caaaagccgg gtcatcggac ggttgcgcca gcgtggcgtg caattgcgcc agacgcctgc
     1561 caatcgccga ggccacggcg gcatagccat gcaattcttc ctcatagtcc ggctggcctt
     1621 cctcggtcag gccagtgttt tccagggtgc gcttcaaata gtcctgggtc cacgcccacg
     1681 cgtcgccctg gttgccgatt tgcgcatgca acagagccaa cgtatgtggc gtcccgtcgg
     1741 gattgacgcg aatcagctcc cccagcagtt ccgccgaatt ggtgtaaccg gcgagcctca
     1801 agtggcgcgt catttcggcc tcgggatgga ttccgggaac aacgtgacgc atcagcttta
     1861 aaattgcagt gtggcccaat gtcattgaac tgttggactg ttctccgtga aaccaatcca
     1921 ctgtcagatc ttgcgggaat tgaagtgtgt tcaggccctt ttcgccctga aagcgcagaa
     1981 ggcaacgccc gtcggacgtc tcaaccgaga tctcgcgatg ggcccgcaga ttgtccagca
     2041 gcgtcagggc gaaatcggga acgctgaacg catcggtaac cagccccagc tccgatcctc
     2101 gccgcactcg cgccagacca tattgctgtg gcggtcctga tatcagttca ccccacaaaa
     2161 gcgcaagggg cagaaaatag cgagccgcgc cgtcggcgaa ttcgacctcg aattccgcca
     2221 tgaaaaggtc gggttggccg ggcattggca tgccatagga aatttgcact gcgcgaatcc
     2281 gctgcccgcg cgcaaaccac cgttggcgcg ccagatacaa gggcaacact tcactgcata
     2341 acgtctttct cgacccttcg accagttgat atgcggctcc cgcgcgccga cgcaccacca
     2401 aagtgacata ttccggcatc tgttcgggca tcgtcgcatg ccataccggt ggcgcagcgt
     2461 tgccgcttag ctcgaaccag taaaatccat agggtggcaa agtgagcaga tacggcagtt
     2521 ctccgatggt gggaaatggt gtgcctccca gcagttcaac cggcacgcgg ccagccataa
     2581 acgcgagatc gagctcaacc ggctgcgatg catttgaaag attggccacg catagcagcg
     2641 caacgccatc gtattcgcgc agataggcca gaattttccg gttgcccgga tagagaaacc
     2701 gcaaggtgcc ccgcccgaag gcatgggtct gctggcgctt ggccagcatg cggcgtgtcc
     2761 agttcagcag cgagtgcgga tcgcgttgtt gcgcctcgac gttgaccgct tcgtaaccat
     2821 acagcggccc cataagaaca ggaagcgaca agcgctcggg atccgcgcgc gaaaagccgc
     2881 cattgcggtc cggcgtccac tgcattggcg tgcggactcc gtcgcggtcg cccagatgta
     2941 tgttatcgcc cataccgagc tcgtcgccgt agtacatgac cggcgtgccc ggcatcgaaa
     3001 acagcagact attcatcaac tcaatgcggc ggcgatcccg ttccagcaaa ggcgccaagc
     3061 ggcgccgaat gcccaggttt atgcgcgcgc gacggtctgc ggcataaact tcccacaaat
     3121 aatcgcgttc gctgccggtc accatctcca gcgtcagttc atcgtggttg cgcagaaaaa
     3181 tggcccactg acaattttca ggtatttccg gtgtttgccg caaaatatcg gtaatcggaa
     3241 acctgtcggc ctgcgcaatg gccatataca tgcgcggcat cagcgggaaa tggaacgaca
     3301 tatggcactc gtcgccttta ccgaagtatt cctgcgcatc ttcgggccat tggttggctt
     3361 cggccagcag caaacgatcg ggataatccg catccataac ggcccggatt tggcgcaaaa
     3421 taatgtgggt ctcgggcagg ttttcattgc tcgtgccttc acgttccacc agatacggca
     3481 cggcatccag gcgcaggcca tctaccccca tgtcaagcca gaaccgcata actccgatca
     3541 tttcccgaag cactctggga ttgtcgtagt tcaagtccgg ttgatgcgaa taaaatcggt
     3601 gccagaaata ggcgcccgcc acggggtccc atgtccagtt tgatttctcg gtgtcgcaaa
     3661 agattatgcg cgtgcccgcg taagcctggt cattgtcgga ccacacataa aagttgcggg
     3721 cggcggaacc agcccgggcg tggcgtgcgc gctgaaacca gacatgttgg tccgaagtgt
     3781 gattgatgac cagctcggta atgatgtgca gaccgcgacg atgagccaga cgtatgaact
     3841 ttcttacatc cgacaagacg ccataatcgc tgtgcacacc gcgatattcg gcaatgtcgt
     3901 aaccatcatc gaggcgcgga gacgggtaaa acggaagcag ccagacagtg gtcacaccca
     3961 gctctacgat atagtccagt ttggcagtca agcctgcaaa gtcccccacc ccgtcgttat
     4021 tcgcatcgaa gaacgacttc acatgcaact gataaatgac ggcatctttg taccagagag
     4081 gttcattgct agccgccaca tgcgatggac gtggtgttgc cattgaaacc tctgcgttag
     4141 tcggggcacg tgctggcgtg ccgcccgccg gtgcgcttgt catattgggg gaagtacctt
     4201 ccacagtaaa aagggacgct gcggcgttag ttccaccctg tgatgtttgc ctgaaagggc
     4261 gaaccggcga tcatcaaata aatcttccaa aaggaccgtc gcattgtcgg gcagactcca
     4321 ttgccacagc ggcagttcca gcgtgccgaa ccgcaccgcg taagggtcga ggcttattgc
     4381 cacaagtaca atgctgtcgc gttgcggcgt cgatttcgaa aaaaacaaaa tccggtcatc
     4441 atcgacatga tggaaacgta ttcccaaatg cgattgcaag gcagggttgg tgcggcgaat
     4501 ttgattcagc ttcgttatct ccggcacaat atttcccggg cgattccaat cccatgatcg
     4561 cagttcatat ttttcggaat ccagatattc ttctttgccc ggcaatgcgc gcgcctcgca
     4621 tagttcaaaa ccgttataca taccccatag acccgaagtg gtggccgcca gcgccgcccg
     4681 tatcaaaaag ccggctcgcc cggacgtctg caggaaatac ggattgatgt ccggtgtatt
     4741 aacaaaaaaa tgggggcgaa agaaactcgc gatcgaagga ctgtttagct ccgtcaaata
     4801 gtcgataagc tcttgcttgg tgttacgcca cgtgaagtac gtataggatt gcgaaaagcc
     4861 aatcttcgcc agccgataca tcatcttggg acgcgtgaat gcttcggaca gaaagatggt
     4921 gtcgggatgg gttccctgaa cgtcggaaat cagccactgc cagaacggca gcggtttggt
     4981 gtgcggattg tctacccgaa aaatatgtat tccgtgttcg atccagaaca gcaccacatc
     5041 gcgcaattcg cgccacaacg ccatctgcct ggaggcgctt gccaggctgc tataaaagga
     5101 gggagaaacg atgtcttcgt atcgctttgg cggattctcc gcataacgca acgacccgtc
     5161 cgcgcgccag gaaaaccact cgggatgttg tgcaagccaa gggtgatcag gcgaacactg
     5221 tatggcaaag tcaagggcaa cttccagttc gaactggcgc gcgcgttcga ccaacgcctg
     5281 gaagtcctgc agcgtgccta attgcggatg caccgaggtg tgtccgccta gcaccgaacc
     5341 aatggcatat ggactgcccg ggtcttcggg gcttgccttg agcgaattat tccttccttt
     5401 gcggttgctt agaccgatgg gatgaatagg cgggaagtac agcacgtcaa aacccatgtc
     5461 gcgaatggcc ggcagacgct tcatgacatc cctgaacgtt ccatgaacgc ctggtttgga
     5521 accgcaagat cgcgggaaaa gctcatacca actggcaaag gacgcctcgc gccgctccac
     5581 acctatttcg aaaggtcttt cacacgtggc ctcaaagcgg cggatgtccg tctcccgcat
     5641 tgcagccgcg accgtctcgg acagcagaat ctctacttgt tcgggtcgcg cgggaggcac
     5701 cggcagcccc gcgaccaccg cacattcgct tcgcgccgct gcggcgcgcc tggcctttag
     5761 acgcggggtc tgtcgcggca tgcccacatc gtccagtatt cgtgttaacg attggacata
     5821 ctgtgacgag tcgtccatgg gtctatcgga agttttgcgt ggctggacag catttagcaa
     5881 taatgcgcgc ccttcttcca catccagcga tacgtcctgc cccgccgcga cctttttcgc
     5941 caattgctcg cgataggagc cccaccgatc atgccaggca cgaatcgtga aatagtgctt
     6001 gccgatacgc gttggcgaaa atcgggcttc ccatcggtcg ttttccagca tggtcatgaa
     6061 ggtttcccgc caactcactt cgtccgcggc ccgccacagc atgctggcca gaatgcgctc
     6121 atgtccatcc atgaatatgt cggcctgtac aacaacggtt tcaccaatct gggttttggc
     6181 gggaaatctg cccttgtcga cctctggaat gacaccgtca atggttatac gcgccgcgcg
     6241 caaagcggcc acgactgcct tgcgcttatc ctcggacgta gtggcggtcg cggtcacgca
     6301 ttggtccgaa aacgtcaaaa acaaaaggca cccgccgggc ttcagttctg ccgcatccga
     6361 acagggcaca cccgcagccc cgtccggatc cggcatgacc tggatcatcc cattgtcatg
     6421 cggcagacgg gcgcgtacat cggcccattt tttggtggcg tcaacttgcc catcgggatt
     6481 aatcaacaaa ccggcgcctg atcggaaaag cacggtcgcc ggcgataatg cgcccgataa
     6541 tacgcgcagg cgtccggtcg tgttcggcct gtcctgcaac catttgttca gttgggccag
     6601 cgtcagcgag ccagcaattt tttccagact ggtcgtgacc aggactccgt tgccggctat
     6661 tgcggcagtc caaatcttgc gccgcgcgtc attgccggcc tgcgcaaggt cgaaatcagc
     6721 cggatccacc acagtgtccg ggtctgtgac cggcgaaaat acgggcgcga tggcgccgag
     6781 gcgaacgtat tcgtcgatta accatgtagc gcgataatcc caccatggca aggacgaaaa
     6841 cgtagcgtcg aaaccggcgc ctttcaagcg gtcgatctgc ccagcgccaa gaccaggggt
     6901 ccatgccata aaatgacagt gcggttgcaa cgatctcacg ttatcgatca ggcggcgcca
     6961 ctcgcccctg gatagttctg ccggcgcacg acatcggaaa cccgacacgc cggcccgcac
     7021 ccaaccgccc agacgccggg accaggcttc aataaattca atgcgcgcaa gtccgtcatt
     7081 gccgcgcaca cgcgcaggct cttcgccgac agtcttgtcc aacaccagat cagtcatcag
     7141 atccaaaccg tgttgcgcgc agacagacgc cattccgccc a
//
LOCUS       DATCXU010000322        12024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_110044,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000322 DATCXU010000000
VERSION     DATCXU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110044"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VIR04_07190"
     CDS             <1..639
                     /locus_tag="VIR04_07190"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460565.1"
                     /translation="LLAPTSGQVRVFGETLTGINHRAGYMFQGEALLPWRSALANVTA
                     GLEFAHVPKAQATELGNDWLKRVGLGGFENRYPHQMSGGMRKRAMLAQTLIRDPDIIL
                     MDEPFSALDIQTRQLMENEVLELWMAKRKAVLFITHDLDEAIAMSDRVVVLSAGPATR
                     PIGEFVIDIPRPRDVAEVRTNARFVELHKAIWGVLREEVLKGYAQQKQVHNV"
     gene            632..1426
                     /locus_tag="VIR04_07195"
     CDS             632..1426
                     /locus_tag="VIR04_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460566.1"
                     /translation="MSKPFRSNSPIMRFWQILLLAIIVLLWHFASRNVNFAFFFGQPV
                     EVVQVIWAWFVTNGDIYRHLGITLSETVLAFIIGTVSGLAFGLWLALSDGASAILDPY
                     IKAANSMPRVILAPIFGMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVSTTLLDNA
                     RMLGANRKQLLRQVYIPSATSWVFSSLHTSVGLAFVGAVVGEYLGSANGVGYLILQAE
                     GTLDVNTVFAGIVVLTVFALILDGIVTLVEDHMLAWKPKAGETEKL"
     gene            complement(1437..2396)
                     /gene="tal"
                     /locus_tag="VIR04_07200"
     CDS             complement(1437..2396)
                     /gene="tal"
                     /locus_tag="VIR04_07200"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY9460567.1"
                     /translation="MSNQLDSLRQYTTVVADTSDFEAMRKYRPTDATTNPSLILKAVQ
                     TEACRPMLDKVVTDHPGLASAELADRILVAFGHEILDIIPGRVSTEVDARLSFDTQAS
                     IERARQIIELYRQAGVGRERVLIKVAATWEGIQAARELQAEGIRCNITLLFSLPQALA
                     CAQAKVQLISPFVGRIYDWSKKAAGDKWDESANAGALDPGVQSVKQIYDYYKAFGIPT
                     EIMGASFRNIGQIRALAGCDLLTISPDLLGQLAANNDPLPRRLDVQEARKSAPEWLAS
                     NEVAFRTELNQNAMATEKLSEGIRSFAADAVKLDALIAQARRP"
     gene            2563..3717
                     /gene="carA"
                     /locus_tag="VIR04_07205"
     CDS             2563..3717
                     /gene="carA"
                     /locus_tag="VIR04_07205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY9460568.1"
                     /translation="MLPSFFPEGSSSYSPAILALADGTVFKGVSIGADGHCVAEIVFN
                     TAMTGYQEILTDPSYSGQIITLTYPHIGNTGINEQDAESKRIHAAGLVVRDCSLRVSN
                     FRSTLSLPEYLKSQDIVAISDIDTRKLTRILRDKGAQGACIWVGDSVDQAIRLAKSFT
                     GMTGQDLAKVVSIKAAEPWVDGSWQLNAGFTQPKTGAYHVVAYDFGVKSNILRLLADR
                     GCRVTVVPAQTPAADVLALEPDGIFLSNGPGDPEPCDYAVEITKMALDRKIPLFGICL
                     GQQIMGLALGGKTVKMKTGHHGANHPVQDLDSGRVFITSQNHGFNVDPDTLPSNVRIT
                     HRSLFDGTLQGFELTDSPAFCFQGHPEASPGPHDIIVLFDKFISLMAGKK"
     gene            3727..6966
                     /gene="carB"
                     /locus_tag="VIR04_07210"
     CDS             3727..6966
                     /gene="carB"
                     /locus_tag="VIR04_07210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY9460569.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALKAEHYRT
                     ILVNSNPATIMTDPETADVTYIEPITWQVLEKIIERERPDAVLPTMGGQTALNCALDL
                     AHNGVLEKYGVELIGANEHAIDKAEDRQKFKQAMTSIGLESAKSGVAHSMDEAWDAQR
                     RIAEQVGSSGFPVVIRPSFTLGGSGGGIAYNPEEFEVICRRGLEASPTRELLIEESLL
                     GWKEFEMEVVRDCADNCIIVCAIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASI
                     AILREIGVDTGGSNVQFAVNPADGRMIVIEMNPRVSRSSALASKATGFPIAKVAARLA
                     VGYTLDELKNEITGGATPASFEPTLDYVVTKVPRFTFEKFPQADSRLTTQMKSVGEVM
                     AIGRTFQESFQKALRGLEVGVDGLNQKTTDREKLQIELGEPGPERIWYVGDAFAQGFS
                     LEEVHDLTRIDPWFLAQIKEIVDIELALEQKTLAELDFDDIWQLKRHGFSDRRLAFLL
                     DTSETEVRKLRHQLGIRPVYKRVDTCAAEFATNTAYMYSTYEEECEARPTDRKKIIVL
                     GGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTL
                     EDVLEIVHKEKPAGVIVQYGGQTPLKLARALETNGVPIIGTSPESIDVAEDRERFQKL
                     LNKLGLRQPVNRTARTEGEALIHAAEIGYPLVVRPSYVLGGRAMEIVYGQQDLERYMR
                     EAVKVSNDSPVLLDHFLNNATEVDVDCIADGERVFIAGVMEHIEQAGVHSGDSACSLP
                     PYSLSPEIVNEIKRQTSLMAHALNVKGLMNVQFAIQNGDVYVLEVNPRASRTVPFVSK
                     ATGLQLAKVAARVMAGTTLAAQGVVKEITPPYFSVKEAVFPFIKFPGVDTILGPEMKS
                     TGEVMGVGKSFGEAFIKSQLASGASLPESGVVFISVKDEDKPHAIDVARGLIALGFTL
                     VATRGTATAIRAAGLDVQIVNKVTEGRPHIVDMIKNGEISLVINTVDERRNAIADSRL
                     IRTHALAARVTFFTTIAGAMAAEQGLQYRRSSDGTKVYSLQSLHASIQA"
     gene            6985..7740
                     /locus_tag="VIR04_07215"
     CDS             6985..7740
                     /locus_tag="VIR04_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9460570.1"
                     /translation="MMRVFITGASSGIGAALAAVYARRGAQLALIGRRERALLDLQDI
                     LPGEHRSYVVDVRNRPALHAAAADFLTLGPVDAVIACAGISAGTLTEELGDYDVFESI
                     IATNLNATVATFEPFISSMKARRQGRLVGISSVAAVRGLPGAGAYSASKAAVKNYCES
                     LRVELSLTGVKVVTLAPGFIQTPMTAHNPYRMPFLMPVGDFAAQAVRAIDRGVSYKVI
                     PWQMAWVAKMLRCIPDRLYDRIAVGRKRKPRNL"
     gene            8055..8531
                     /gene="greA"
                     /locus_tag="VIR04_07220"
     CDS             8055..8531
                     /gene="greA"
                     /locus_tag="VIR04_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY9460571.1"
                     /translation="MSAIPLTARGAERLQKELQRLKSVERPDIIGSIAEARAQGDLSE
                     NAEYDAARERQAFVEGRIRELEGTLSNAQIIDPTALDVDGKAVFGATVEIEDLESGER
                     VTYQIVGDVEADIRANMISVSSPVARALIGKGEGDVVEVKAPAGIREYEVVAVNYL"
     gene            complement(8553..9137)
                     /locus_tag="VIR04_07225"
     CDS             complement(8553..9137)
                     /locus_tag="VIR04_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY9460572.1"
                     /translation="MPKLDITSQERSALRAAAHPLHPVVLIGDRGLSESVLKEIDLNL
                     TAHNLIKVRVGGQERAARDALLETICESLSCAPVHHLGKTFIIYRPDEAAALAKAEAA
                     NATPARRKPSEPHTPKKLAAAGVKRTRRTEKAERAAKKALRNETASVAPRARRAIAAA
                     ESRAGGSDATRRTGSALSLRAGARRNVNSRGFKK"
     gene            9155..9787
                     /locus_tag="VIR04_07230"
     CDS             9155..9787
                     /locus_tag="VIR04_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY9460573.1"
                     /translation="MAKNKFSKEWIQQHINDPYVKLAQQKGYRARAAFKLTEILDTEK
                     LLRRGDVVVDLGSTPGSWSQVVRERLLGPDGIIDGRIIALDMLPMEPVADVEFILGDF
                     REDSVLESLEEMLGNQQVDLVISDMAPNLSGIGSADSARIQHVCDLALDFSCRWLKPE
                     GGLVVKAFHGSGFSQIVESFKRRFRRVIERKPKASRAKSSETFLVGRHLK"
     gene            9930..11825
                     /gene="ftsH"
                     /locus_tag="VIR04_07235"
     CDS             9930..11825
                     /gene="ftsH"
                     /locus_tag="VIR04_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY9460574.1"
                     /translation="MNNSFSKVAIWMVIALVLFTVFKQFDGRPVSADTVTYTQFMNDA
                     QAGRINKVDIQGDTLHVTPDAGRPYVLTSPGDLWMVPDLVKYGVQVSGKAREEPSFLT
                     SLFISWFPMLLLIGVWIFFMRQMQGGGKGGAFSFGKSRARMLDENTNQITFADVAGCD
                     EAKEDVQELVDFLRDPTKFQRLGGRIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFENAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFETGQGVLVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILK
                     VHMRKVPLATNVDALVLARGTPGFSGADLANLVNESALFAARRNGRTVEMNDFEKAKD
                     KIIMGAERRSLIMPEEERKNTAYHESGHALVACSLPKTDPVHKVTIIPRGRALGVTMQ
                     LPEGDRYSMDKERLLNTIAVLFGGRIAEEVFMHQMTTGASNDFERATAIARDIVTRYG
                     MTDSLGPVVYAENEGEVFLGRSVTKTTHVSEATMQKVDQEIRSIIDTQYIVARDIIEN
                     NRDKMEAMAAALLEWETIDADQIQDILQGRPARPPKVMPPPSEPSDHTPPSGLAPADG
                     SAAATPV"
     gene            11843..>12024
                     /locus_tag="VIR04_07240"
     CDS             11843..>12024
                     /locus_tag="VIR04_07240"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY9460575.1"
                     /translation="MSATLLCGRFELTFERPLIMGIINVTPDSFSDGSQHFETDSAIA
                     YAKTLIAQGADILDIGG"
BASE COUNT         2529 a         3185 c         3538 g         2772 t
ORIGIN      
        1 ttgctggctc ccacatcggg ccaggtcagg gtctttggcg agacattgac cggcattaat
       61 catcgcgccg ggtatatgtt tcagggcgaa gcgttgttgc catggcgaag cgccctggcc
      121 aatgtgacag ccggcctcga attcgcccat gtgccgaaag cacaggcgac ggaactcggc
      181 aacgattggc tgaaacgagt aggcctgggt ggattcgaaa accgttatcc gcaccagatg
      241 tcgggcggca tgcgcaagcg cgccatgctg gcgcaaactt tgatccgcga ccccgacatt
      301 attcttatgg acgagccgtt ttccgcgctc gatattcaga cgcggcaatt aatggaaaat
      361 gaagttcttg aactctggat ggccaagcgc aaggccgttt tgttcatcac gcacgatctt
      421 gacgaggcca tagccatgag cgatcgcgtg gttgttcttt cagcaggccc ggccacccgc
      481 ccgatcggtg aatttgtcat cgatattccg cgacccagag atgtcgccga agtccgtacc
      541 aacgctcgat ttgttgaatt gcacaaggca atatggggcg ttttgcgcga agaagttctg
      601 aagggctacg cccagcaaaa gcaggttcac aatgtctaaa ccctttagat caaactcgcc
      661 cataatgcgc ttttggcaga tattgctatt ggccataatc gttttgttgt ggcatttcgc
      721 ttcgcgaaac gtcaatttcg ccttcttttt cggccagccg gtcgaagtcg tgcaggtgat
      781 atgggcctgg ttcgtgacca atggcgatat ctaccggcac ctgggcatca ccctgtcgga
      841 aaccgtgctg gcttttatca tcggaacggt atccgggctg gcgtttggcc tttggcttgc
      901 gctgtctgat ggcgccagcg caatcctcga cccgtacatc aaggccgcca actcaatgcc
      961 acgtgtgatt ctcgcgccca tttttggcat gtggttcggg ctgggtatct ggtccaaagt
     1021 ggctctggcc gtcacactgg tgtttttcat tgtatttttc aatgtctatc agggggtgcg
     1081 cgaggtcagc accaccttgc ttgataacgc ccgcatgctg ggagccaacc gcaaacagtt
     1141 gttgcgtcag gtctatattc catcggcaac cagttgggtg ttttcaagcc tgcatacatc
     1201 ggttggtctg gcattcgtcg gcgccgtggt cggcgagtac ctgggttcgg ccaacggggt
     1261 cggatatctt attctgcaag ccgaaggtac gctcgacgtg aatacggtat ttgcgggcat
     1321 tgtggtgctt acggtatttg ccctgatcct ggacggtatt gttacgctgg tcgaagatca
     1381 tatgcttgca tggaagccca aggccggcga aacggagaag ctgtaggaag ccttggtcag
     1441 ggtcgccgcg cctgcgcgat cagcgcgtcc agcttgaccg catcggccgc aaatgaacgt
     1501 atgccttcgg acagtttttc tgtcgccatc gcattttgat tgagctcggt gcggaaagcc
     1561 acctcgttgc tggccagcca ttcgggcgcg ctctttctgg cttcctgtac atcgagccgg
     1621 cgcggcaaag gatcgttatt ggctgcaagc tgccccagta gatcggggct gatggtcagt
     1681 aaatcacagc ccgcaagcgc ccgaatctgg ccgatattgc gaaagctggc tcccatgatt
     1741 tcagtcggta tgccaaatgc cttgtaataa tcatagattt gcttcaccga ctgcacgccg
     1801 gggtctaacg cccccgcatt ggccgattca tcccacttgt cgcctgccgc cttcttggac
     1861 cagtcgtaga tgcggccaac gaaaggggaa atcagttgca ctttggcttg cgcacaggcc
     1921 agagcctggg gtaatgaaaa gagtagtgtg atgttgcagc gaatcccctc ggcctgaagc
     1981 tcgcgggcgg cctgaatgcc ttcccaggtt gcggcaacct tgatcaagac gcgttcgcgc
     2041 ccgacccccg cctggcgata cagttcgatg atctgccgcg cacgttcgat gctggcttgc
     2101 gtgtcgaacg aaagccgtgc gtcgacctcg gtcgaaactc gtcccggaat aatatcaaga
     2161 atctcgtgtc cgaaagcaac cagaatacgg tcagccagct ccgcgctggc caagccagga
     2221 tgatcggtca caaccttgtc aagcatgggc cgacaggctt ctgtttgcac tgccttcagg
     2281 attagcgatg gatttgtcgt cgcgtcggtc gggcggtatt tgcgcatggc ttcgaagtcg
     2341 ctcgtatcgg caacgacggt tgtgtattga cgtaaagaat cgagttgatt ggacatggta
     2401 atcacccagt ggcggcgcga caggaaatgg acttgctact gtagaccatt ttttacgttt
     2461 tggtccgata gcgagattgg gcggcggatg ctcgtcggaa tggtggcctt ctcaaggtat
     2521 aatcccaagg tttagttatc gattttctaa ccggggtttt ctttgctacc gtcatttttc
     2581 cctgaaggat ccagttcgta ttctcccgct attttggccc tggcggacgg tactgtcttc
     2641 aagggtgttt ccatcggcgc cgatggccac tgtgttgccg aaattgtttt caataccgcc
     2701 atgacgggtt atcaggaaat cctgacagat ccgagctata gcgggcaaat catcacgctg
     2761 acatatccgc atattggaaa taccggcatc aacgagcaag atgccgaatc caaacggatt
     2821 cacgcggccg gactggttgt gcgcgattgt tccttgcgtg tttccaattt ccgttcaacg
     2881 ctgtctttgc ccgaatacct gaaatcgcag gacattgttg cgatttcaga tattgacacg
     2941 cgcaaactga cgcgaatttt gcgcgacaag ggcgcccagg gcgcgtgtat ttgggtaggc
     3001 gatagcgtcg atcaggcgat caggcttgcc aaatcattca caggcatgac ggggcaggac
     3061 ttggccaagg tggtttcgat taaagccgcc gagccatggg tcgatggttc gtggcagttg
     3121 aatgcaggct tcacgcaacc gaaaacgggc gcttatcacg tcgtagcgta cgactttggc
     3181 gtcaagtcga acatattgcg cctgctggct gatcgcggct gccgcgtcac cgtggttcca
     3241 gctcaaaccc cggccgccga tgtgctggcg ctcgaaccgg atggaatatt cctttccaac
     3301 gggccgggcg accccgaacc ctgcgactac gcagtcgaaa taaccaaaat ggctttggac
     3361 cgcaagattc ctctattcgg gatttgcctg gggcagcaga tcatgggcct ggcactgggc
     3421 ggcaaaacag taaaaatgaa gaccggccat cacggcgcca accatcctgt ccaggatctg
     3481 gattcggggc gcgtattcat caccagtcag aatcatgggt tcaatgtcga tcccgatacc
     3541 ctgccttcca atgttcggat aacccaccgc tccctgttcg atgggacttt gcaggggttc
     3601 gaacttaccg atagccccgc cttctgtttt cagggccatc ccgaagcaag cccgggcccg
     3661 cacgacatca tcgtgctgtt cgacaaattc atcagcctta tggccggaaa aaaataaatc
     3721 agcattatgc ccaagcgtac agatataaaa agcatactca ttattggtgc aggcccgatt
     3781 gtcattggtc aggcgtgcga atttgattac tcgggagcgc aagcgtgcaa ggcgctcaag
     3841 gccgaacact atcgaaccat tctggttaac agtaacccgg ccaccatcat gaccgacccc
     3901 gaaacggcgg acgtgactta tatcgagcca atcacctggc aggttcttga aaaaatcata
     3961 gagcgtgaaa gacccgatgc cgtgttgccg accatgggcg gccagaccgc cctgaattgc
     4021 gcacttgatc ttgctcataa tggcgtgctg gaaaaatacg gtgtcgagct tattggcgcc
     4081 aacgaacacg cgattgacaa ggcggaggat cgtcagaaat ttaaacaggc catgacgtcg
     4141 ataggcctgg aatcggccaa atcgggcgtg gcgcattcaa tggacgaagc ctgggatgcg
     4201 caacgccgca ttgcggaaca ggtcgggtct tcaggctttc cagtggttat ccgtcccagc
     4261 tttacgctag gcgggtcggg gggcggaatt gcttacaacc ctgaagaatt cgaggtcatt
     4321 tgccggcgcg gactggaagc gtcgcccacc cgggaattgc tgatcgagga atcgctgctg
     4381 ggctggaaag aattcgagat ggaggtcgtg cgcgactgtg ccgataattg cattattgtc
     4441 tgcgcgatcg agaacctgga tccgatgggg gtgcataccg gcgattcgat tactgtcgcg
     4501 cctgcacaaa ccctgaccga caaagaatat caaatcatgc gtgacgcatc gattgcgata
     4561 ttgcgcgaaa tcggcgtcga taccggaggg tcgaacgttc agtttgcggt gaaccccgca
     4621 gacggccgca tgattgtcat cgaaatgaat ccgcgtgttt cgcgttcctc cgcattggct
     4681 tccaaagcaa ccggtttccc gattgccaag gtggcggctc gcctggcggt gggctacacg
     4741 cttgacgaac tgaaaaacga aatcacgggc ggcgccactc ccgcttcgtt cgagcccacc
     4801 cttgactacg ttgtaaccaa ggtgccgcgc tttacgttcg aaaaatttcc ccaggccgat
     4861 tctcgtttga caacgcagat gaagtcggtg ggcgaggtca tggccattgg ccgcactttt
     4921 caggaatcct ttcaaaaggc tttgcgcggc cttgaagtcg gtgttgacgg cctgaatcag
     4981 aaaaccacgg atcgggaaaa gttgcagatt gagctgggcg aacccggccc cgaacgtatc
     5041 tggtatgtgg gcgacgcctt cgcgcaaggc tttagccttg aagaagtgca cgaccttacg
     5101 cgtatcgatc catggttcct ggcgcagatc aaggaaatcg tcgatatcga actggctctc
     5161 gagcaaaaaa ccctggccga gctcgatttc gacgatatct ggcaattgaa gcgccacggc
     5221 ttttcagacc ggcggcttgc ttttttgctg gatacgtccg agaccgaagt tcgcaaactg
     5281 cgccatcaac tgggcatacg ccccgtgtat aaacgagttg acacttgcgc ggccgagttc
     5341 gccaccaaca ccgcttatat gtattcgact tacgaagaag aatgcgaagc gcgccccacc
     5401 gatcgcaaaa agatcattgt gttgggcggc gggccgaacc ggatcggcca gggcattgaa
     5461 ttcgattact gttgcgtgca tgcggcgctt gcgttgcgcg aagatggtta cgaaaccatc
     5521 atggtcaatt gcaaccccga aacggtgtca accgactacg atacctccga tcgcttgtat
     5581 ttcgagcccg ttactctgga agatgtgctc gagatcgtac acaaggaaaa accggcaggt
     5641 gtgattgtgc agtacggcgg tcagactccc ttgaaacttg cgcgggcgct tgaaaccaat
     5701 ggtgtgccaa tcattggaac cagccccgaa tcgattgatg tcgccgagga ccgcgagcgt
     5761 ttccagaagc tgctcaataa gctgggcctg cgtcagccgg ttaatcgcac ggcgcgcaca
     5821 gaaggtgaag cactgattca tgccgccgaa atcggctatc cgctggtggt tcgcccaagc
     5881 tacgtgctgg gtgggcgcgc gatggaaatc gtgtatgggc agcaggatct tgagcgctac
     5941 atgcgcgaag ccgttaaagt cagcaatgac tccccggttc tgctggatca cttcctgaat
     6001 aacgccactg aagtcgacgt ggattgcatc gccgatggcg aacgggtgtt catcgccggc
     6061 gttatggaac acattgaaca ggcgggcgtt cattcgggcg attcagcgtg cagcctgccg
     6121 ccttattctt tgtcgcccga gattgttaac gagataaaac gtcagacctc gcttatggcg
     6181 cacgccttga atgtgaaagg cctgatgaac gttcagtttg ccatacagaa tggcgatgtt
     6241 tatgtgctgg aagtcaatcc acgcgcatcg cgcaccgtgc cttttgtttc caaggcgaca
     6301 ggacttcaat tggcgaaagt cgcggcgcgg gtcatggcgg gcaccacgct ggccgcgcag
     6361 ggcgttgtga aggaaatcac gccgccttat ttttccgtca aggaagcggt ttttccgttt
     6421 attaagttcc ccggcgtcga cactatcctg gggccggaaa tgaagtcgac gggcgaggtc
     6481 atgggggttg gaaaaagttt tggcgaagcc ttcatcaagt cgcaacttgc ttcgggcgcg
     6541 tctctgcctg aatcaggcgt tgttttcatc agcgttaaag atgaggacaa gccgcatgcc
     6601 attgacgttg cgcgcggctt gatagcgctt gggtttacgc ttgtcgccac ccgcggtacg
     6661 gcgacggcaa ttcgggcggc tggcctggat gtgcaaattg tcaataaagt gaccgaaggg
     6721 cgccctcaca tcgtcgacat gatcaagaac ggcgagattt cgctcgtcat taataccgtt
     6781 gatgagcgcc gcaatgccat tgccgattcg cgccttatcc gcacccatgc gctggccgcg
     6841 cgggttacgt tctttacgac catcgcgggc gcgatggctg ctgaacaagg gctgcaatat
     6901 cgtcgcagca gtgatggcac gaaggtgtat tccctgcaat cgctgcatgc ctcgattcag
     6961 gcctgacagg atgcggcggc gcccgtgatg cgcgtattta tcacgggcgc cagcagcgga
     7021 ataggtgcgg ctctggcggc tgtgtacgcg cgcaggggcg ctcaattggc cctgattggc
     7081 cggcgtgaac gggctcttct ggatttgcag gacattctgc ccggcgagca tagaagttat
     7141 gtggtggatg tcaggaatcg gccggccctg catgcggcgg cggccgattt tttgaccctg
     7201 ggacccgtcg atgccgttat tgcctgcgcg gggatcagcg ccggcacgct aaccgaagaa
     7261 ctgggcgatt acgacgtctt tgaatcgatc atcgccacga acctgaatgc gacggtggcc
     7321 acgttcgagc cgtttatctc atcaatgaag gcgcgccgcc agggccgtct ggtgggtatt
     7381 tccagcgtcg cggcggtgcg cggactgcca ggggccggcg cctacagcgc ttcgaaagcg
     7441 gcagtgaaaa attactgtga aagccttagg gtggaactga gtttgaccgg cgtcaaagtg
     7501 gtgacgcttg ctccagggtt tattcagacc cccatgacgg cccataaccc gtacagaatg
     7561 ccgtttctta tgccggtcgg cgattttgcg gcgcaggcgg tgcgtgccat tgacaggggc
     7621 gtctcgtaca aggtcattcc gtggcaaatg gcatgggtgg ccaaaatgtt gcggtgcatc
     7681 cccgatcgtt tgtatgatcg tattgcggtg ggccgcaagc gcaagccaag gaacctgtaa
     7741 cggcgtgcta taaacaaggc tgccaacgcg cggttccgcg tttcttttca tcaacttgcg
     7801 cggtgtgttc gggtaaacgg ccgagcatct tctggcgggc ttgcaatttc ctccgacatt
     7861 gccgacaata gactcaaata gtggttcatg accactaccg gtatttttac ataccggtta
     7921 agttgctgta tagccagcca taacaattca acgcttcttt aagtttcctt ccttgaacgg
     7981 ttgttaactg ttcacgtact tactacaagg cgctccttta tggggagcgt ttttactttt
     8041 gattcaggaa tagtatgtct gcgatcccat tgacagctcg tggtgctgag cgcttgcaaa
     8101 aagagttaca acgtttaaag agtgtcgagc gtccggatat aattggctcg attgccgagg
     8161 ctcgcgccca gggcgatctc tctgaaaatg ccgaatacga tgcggcccgc gaacgtcagg
     8221 cgtttgttga aggccgtatc cgcgaactgg aaggcacctt gtcgaatgcc caaatcattg
     8281 acccgacggc gcttgacgtt gacggcaaag cggtgtttgg cgccactgtc gaaatcgagg
     8341 atcttgaatc gggtgagcgt gtaacctatc aaatcgtggg cgacgttgag gccgacattc
     8401 gcgccaacat gatttccgtt tccagcccgg ttgcgcgtgc gctcattggc aaaggcgagg
     8461 gcgacgtggt cgaagtcaag gcgcccgcgg gtattcggga atacgaagta gtagcggtca
     8521 attatcttta aagccccgtt caagcctgac gactattttt taaacccgcg gctgttcacg
     8581 ttgcgccgcg cgccggctcg aagcgacagc gcgctgccgg tgcggcgcgt ggcgtctgat
     8641 ccaccggcgc ggctttcagc ggcggcgatt gcgcgccggg cgcgtggcgc tacactggcg
     8701 gtttcgttgc gtagcgcctt tttggcggcg cgctcggctt tttcagtgcg ccgggttcgt
     8761 ttcacgcctg ccgcggccag ctttttcggc gtgtgcggtt cggacggctt gcgccgggcg
     8821 ggtgtggcgt tggcggcctc ggccttggcg agcgcggccg cttcgtcggg acggtaaata
     8881 atgaatgttt tacccaggtg gtggacaggg gcgcacgata gcgattcgca aattgtttca
     8941 agcaaggcgt cgcgcgcggc gcgttcttga ccaccgacgc gaaccttgat caggttatga
     9001 gccgtcaggt taaggtcaat ttccttgagc accgattcgg ataagcctcg gtcgccaatc
     9061 aatacgactg gatgcaaagg gtgggcggcg gcgcgcaatg cgctccgctc ttgggaagta
     9121 atgtcgagtt ttggcataat gagattgtac gggaatggcc aaaaataaat tctctaaaga
     9181 gtggatacag caacacatta acgaccctta cgttaaattg gcgcaacaaa aggggtatcg
     9241 cgcccgggcg gcattcaagc tgaccgaaat actcgatacc gaaaaattgt tgcgccgtgg
     9301 cgatgtggtg gttgacctgg gatcgactcc gggcagttgg tcgcaggtgg tgcgcgagcg
     9361 tttactcggc cctgacggga ttatcgacgg ccggatcatt gcattggaca tgttgcccat
     9421 ggagccggtc gcggacgttg aattcatact gggggatttc cgcgaggatt ccgtgctgga
     9481 aagtttggaa gaaatgttgg gaaatcagca agttgacctt gtgatttccg atatggcccc
     9541 caacttgtcc ggcatagggt cggccgattc agctcgaatt caacatgttt gcgacttggc
     9601 gctggatttt tcatgccggt ggctgaagcc ggagggcggt ctggtagtca aggcatttca
     9661 tggcagcggg ttttctcaaa tagtcgagtc tttcaagcgg cggttccggc gcgttatcga
     9721 gcgaaaaccg aaggcgtcgc gggcgaaatc gtcggaaacc tttttagtgg gtcgtcattt
     9781 gaaataaaag gaatatttcc cgggatctgg ccgcgggatt cctgaacaag attctttcta
     9841 tcgggttaga atgagtcatt gacatatttg ttttagcgcc gttgccgcag ggcgcttggc
     9901 aagctgaagt tactcgttgg aggttggctt tgaacaactc gttttccaag gtcgcaatct
     9961 ggatggtgat tgcgcttgtg ctgtttactg tattcaagca gtttgacggg cgccccgtgt
    10021 ccgcggatac cgtaacctac acccagttta tgaacgatgc gcaagcgggc cgcatcaaca
    10081 aggtcgacat acaaggcgat acattgcatg taacgcccga cgcgggccgg ccctatgtat
    10141 tgacgtcgcc gggcgacctc tggatggtgc ccgacctggt caagtatggc gtgcaggtgt
    10201 cggggaaagc gcgcgaggaa ccctcgttcc ttacgagttt gttcatctcg tggttcccca
    10261 tgctgttgtt gattggcgtc tggatctttt ttatgcggca aatgcagggc ggcggcaaag
    10321 gcggggcgtt cagctttggc aagtcgcgcg cgcgcatgct cgatgaaaac accaaccaga
    10381 ttaccttcgc tgatgtcgcg ggttgcgacg aggccaagga agatgtgcaa gaattggtcg
    10441 attttctgcg cgatccgacc aagtttcaac gtttgggcgg gcgcatacct cgcggcgtgt
    10501 tgatggtcgg ttcgcccggc acaggtaaaa ctttgctggc caaggccatt gccggcgagg
    10561 ccaaggttcc gtttttcagc atttcgggct cggacttcgt cgaaatgttc gtcggcgttg
    10621 gcgcagcgcg cgtgcgcgac atgttcgaaa atgccaaaaa gcaagcgccg tgcatcatat
    10681 tcattgacga aatcgacgcg gtgggccgcc agcgcggggc cgggctgggc ggcggcaacg
    10741 atgaacgcga gcaaacgctt aatcagttgc tggttgaaat ggatggcttc gaaaccggcc
    10801 agggcgtgct ggtgattgca gccacgaacc ggcccgatgt cctggatccg gcactgcttc
    10861 gccccggacg gttcgatcgt caagtggttg tgcctttgcc cgatattcgc ggccgcgagc
    10921 aaattttgaa agtgcacatg cgcaaggttc cactggcgac caatgtcgac gcactggttc
    10981 tggcgcgtgg caccccgggg ttttcgggcg ccgatctggc gaatctggtc aatgaatccg
    11041 ccttgttcgc ggcgcggcgc aacggccgca ccgtcgaaat gaacgatttt gaaaaggcta
    11101 aagacaaaat cataatgggc gcggagcgcc gctcgttgat catgcccgaa gaagagcgta
    11161 aaaacacggc ttatcacgag tcggggcatg cgcttgttgc atgctcgctg cccaaaaccg
    11221 atcccgtgca taaagtcaca attattccgc gcggacgcgc cttgggcgtc accatgcaat
    11281 tgcccgaagg cgaccgctac agcatggata aagagcgttt gctgaacacc attgcggttc
    11341 tgttcggcgg tcgaatcgcc gaagaagtat tcatgcacca aatgacgacg ggtgcttcga
    11401 acgatttcga gcgcgccacg gccattgcgc gcgatatcgt gactcgttat ggcatgaccg
    11461 attccctcgg ccctgtggtt tacgccgaaa acgagggtga ggttttcctg ggtcgcagcg
    11521 ttacaaaaac aacccatgtt tccgaagcga ccatgcaaaa ggtcgatcag gaaatccgct
    11581 ccatcatcga cactcaatat attgttgcgc gcgacattat cgagaataat cgcgacaaga
    11641 tggaagctat ggcggcggcg cttttggagt gggaaacgat cgatgccgat cagattcagg
    11701 acatcttgca gggtcgcccc gcgcgtccgc ccaaagtaat gccgccgcct tccgagccat
    11761 cggaccacac gcctccttcc ggcctggcgc cggccgatgg cagcgcggcg gctacgcccg
    11821 tttagggttt cctctcacgg caatgagcgc aactttgctc tgtgggcgtt tcgaactgac
    11881 gttcgaacgc ccattgataa tggggatcat caatgtgacc cccgattcct tttcagatgg
    11941 ttcacagcat tttgaaacgg attcggccat tgcttacgcg aaaaccttga ttgcgcaggg
    12001 ggccgacatt cttgatatcg gcgg
//
LOCUS       DATCXU010000323         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_167943,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000323 DATCXU010000000
VERSION     DATCXU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167943"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VIR04_07245"
     CDS             <1..739
                     /locus_tag="VIR04_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY9460576.1"
                     /translation="AAAKARPDGYTLVVHSDGYSIAPAIYSKLPYDVQKDFTPVALLA
                     RAANAVLVSADSPYKSLQQLIDAGTVPHKLSFATAGVGSAQHMQAATFCAMAHIQEPV
                     HVPFKGTPEALNDVIAGRVDFIFAPLSTAMPMIISGKLRPLAISTAKRSELLKDVPTV
                     AEAGVPGFAEEQWWGMFAPSKVPAAVLQKLETETEVALKTPAMLKLIDSLSSTPGDLF
                     GNDFGKLVDTSIASNKAAAKAYNIHVN"
     gene            876..1937
                     /locus_tag="VIR04_07250"
     CDS             876..1937
                     /locus_tag="VIR04_07250"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY9460577.1"
                     /translation="MQKIKVAVIPGDGIGKEVVPEGIRVLEAAASRFGFSLEWDHFPW
                     SCEYYAKHGEMMPKDGLDKIRHHDAIYLGAVGFPGVPDHISLWGLLIPIRREFQQYAN
                     VRPIRLMPGIKSPLAGRNVGDIDFMVVRENTEGEYSSIGSRQFEGTDDEVATQQSTFT
                     RKGVDRILRYAFEMARKRPNKHLTSATKSNGISVTMPYWDERVAAMAKQYPDVKVDQY
                     HIDILTAHFVLHPHWFDVVVGSNLFGDILSDLGPAVAGTIGIAPSANLNPERDFPSMF
                     EPVHGSAPDIYGQGIANPIGQIWSGAMMLDHAGHPEAGTAIVAAIEKVLISGPRTRDI
                     GGTASTSDVGKAIAQAVKG"
     gene            2033..>2463
                     /locus_tag="VIR04_07255"
     CDS             2033..>2463
                     /locus_tag="VIR04_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460578.1"
                     /translation="MNGINAFQILNGLTFAALLFIVASGFTLIFGLLRIVNLSHGVLY
                     LVGGYIGYVITVHSGNFFIGAAVAMFGVALIGLLLDQGLLRFVRDSELKQVLLTLGVA
                     FVLNDAALVIFGGDTYTVPSPEFLHGAVQFAGLFYPKYRLFV"
BASE COUNT          559 a          723 c          661 g          520 t
ORIGIN      
        1 tgcggccgcg aaagcgcgac ccgacgggta cactctggtg gtccattcag atggctactc
       61 gatcgctcca gcgatatact ccaagctgcc ctacgacgta cagaaggact tcacacctgt
      121 cgccctattg gcgcgcgcgg ccaatgcggt attagtgtcc gccgacagtc cctataaatc
      181 cttgcaacaa ctcattgatg caggcacagt gcctcacaag cttagcttcg cgacagcggg
      241 cgtcgggagc gcgcagcata tgcaggcggc cacattctgc gccatggcgc atattcaaga
      301 accggtgcat gtgccattca aaggcactcc ggaagcgctg aacgacgtca tagccggccg
      361 cgtcgatttt atcttcgcac cgctgtcgac agccatgccc atgatcatct ccggcaaact
      421 cagaccactg gcaatttcaa cggcgaaacg ttcggaactg ctcaaagatg tgcccactgt
      481 cgccgaggcg ggggttccgg ggtttgccga ggagcaatgg tggggaatgt tcgctccttc
      541 caaggtgcct gcggcggtat tacaaaagct cgaaaccgaa accgaagtag cattgaagac
      601 gccggccatg cttaagttga ttgatagcct ctcatctacg ccgggcgact tgttcggcaa
      661 tgattttgga aagcttgtcg acaccagcat cgcctcgaac aaggcagcgg caaaggcgta
      721 caacattcac gtaaattgag gcgaaaacgc gatctaaaga atagagggct ggatcacgtg
      781 aatcgcgaac agcccatagc cttgccctaa attgaaaatc gagcataata acggggctgc
      841 agttcatcga tttcccagta ttcttaagag cttccatgca aaaaattaaa gtggccgtca
      901 tccccggcga tggaattggt aaagaagtag tgccagaagg tattcgcgtg ctcgaggcgg
      961 cggcaagccg ctttggcttt agtttggaat gggaccactt cccgtggagc tgcgagtact
     1021 acgccaaaca cggcgaaatg atgcccaagg acggtctgga caaaatccgc catcacgacg
     1081 cgatttacct cggcgccgtg ggatttccag gcgttccgga ccacatatcc ctatgggggc
     1141 tgcttattcc cattcgccgg gaattccagc aatacgcgaa cgtgcggcca atacgtctta
     1201 tgcccggcat caagtctcca cttgccggcc gcaacgtcgg cgacatcgac ttcatggtcg
     1261 tgcgtgaaaa cacggaaggc gaatattcat ccatcggaag ccgccaattc gaaggcaccg
     1321 acgacgaggt ggccacgcag caaagcacct tcacgcgcaa aggcgtggac cgtatcctcc
     1381 gatacgcgtt cgaaatggcg cgcaagcgcc ccaacaaaca tctgacgtcc gccaccaaat
     1441 cgaacgggat ttcggtcacg atgccctact gggacgagcg cgtcgccgcc atggccaaac
     1501 agtaccccga cgtcaaggtc gatcaatatc atatcgacat cctgaccgct catttcgttc
     1561 ttcatcccca ttggttcgat gtggttgtcg gctccaacct gtttggcgat attttgtccg
     1621 acctcgggcc ggccgttgcc ggcacgatcg gaatcgcccc ctcggccaat ctgaaccctg
     1681 aacgggattt tccgtcgatg ttcgaaccgg tccacggctc ggcgcccgac atctacggcc
     1741 agggtatcgc gaaccccatc ggtcagatct ggtcaggcgc gatgatgctg gaccatgcgg
     1801 gccatccgga agcgggcacc gccatcgtgg cggccatcga aaaagtattg atcagcggcc
     1861 caaggacgcg ggatattggg ggcaccgcca gcaccagcga cgttggcaaa gcaatcgccc
     1921 aggccgtcaa gggctgacac gccgataacc cggcaaacca tccttaaccc attaaagcag
     1981 gaagcgacac acacccaaga tacgaatcca tacaaagggc atcaggcgac aaatgaacgg
     2041 catcaatgcg tttcaaatcc tgaacgggct gaccttcgca gctctgctgt ttattgttgc
     2101 ctctggattc acgctgatat tcgggctttt gcgtatcgtc aacctatccc acggggtgct
     2161 ttacctggtt ggcggttata tcggctacgt gataacagtc cattcaggca actttttcat
     2221 cggtgcggcc gtcgccatgt ttggtgttgc cttgattggg ctgctgctcg atcaggggtt
     2281 gctgcgtttc gtgcgcgatt ccgaactcaa gcaagtcctg ctcacattgg gcgtggcctt
     2341 cgttcttaac gacgctgccc tggtcatatt cggcggcgat acctacactg tgccatcgcc
     2401 cgaattcctt cacggcgccg tccagttcgc gggtctgttt tatccaaaat accgcctgtt
     2461 tgt
//
LOCUS       DATCXU010000324         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_782093,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000324 DATCXU010000000
VERSION     DATCXU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782093"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VIR04_07260"
     CDS             complement(<1..721)
                     /locus_tag="VIR04_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY9460579.1"
                     /translation="MAVNLIVPAETDIFPVAGIEIGIAQAGIRKANRRDLTVFQLAPG
                     VTVAGVFTQNRFRAAPVQVCEAHLATDNQIRALVINTGNANAGTGADGLKAARQTCAA
                     LAELLHVQAKQVLPFSTGVILEPLPVDRLISALPDAVKNLHAANWFAAAHSIMTTDTQ
                     PKIVSRRLNIGGKTVTITGISKGAGMIHPNMATMLGYLATDAGIAPALLKEMIRAIAD
                     RSFNRITVDGDTSTNDSFIIMA"
     gene            complement(769..2067)
                     /gene="hemL"
                     /locus_tag="VIR04_07265"
     CDS             complement(769..2067)
                     /gene="hemL"
                     /locus_tag="VIR04_07265"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY9460580.1"
                     /translation="MSRNTELFERACRTIPGGVNSPVRAFRSVGGSPRFITRAQGPYL
                     WDAEGVRYIDYVGSWGPAILGHAHPEVVRAVQEASVNGLSFGAPTEAEIDIAETIVAR
                     IPSIEKIRLVSSGTEATMSAIRLARGFTGRPKIIKFEGCYHGHADSLLVKAGSGMLTF
                     GQPTSAGVPPEFIQHTLVLDYNYLDGVEAAFKQYGSEIACIIVEPIVGNMNLIKPVPG
                     FLEGLRLLCTQYGSLLIFDEVMTGFRVGPQGVQGLAGITPDITTLAKVIGGGMPVGAF
                     GGRADIMDFIAPLGPVYQAGTLSGNPVAVAAGLATLKLLSEPGFYEKLGTQTGKLAQG
                     LRERAVAANVPFCTDHVGGMLGLYFSETIPTGFSEVSKCDVELFKEFFHAMLDKGIYL
                     APSAFEAGFVSASHDDAIIQQTLDAAEQVFGKLKTRRSTR"
     gene            complement(2160..>2671)
                     /locus_tag="VIR04_07270"
     CDS             complement(2160..>2671)
                     /locus_tag="VIR04_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY9460581.1"
                     /translation="RLPQARELAARCKKLGVVFIVNDDWRMAAAVDADGVHLGREDGP
                     LSEARQALGFGKIIGRSCYNDLTLARQALADGADYVALGALHPSRIKPDAVRATLQHV
                     QQARLLAEQAVTPASPVRAAIIAIGGITPENAAPIIQAGADSIALISALFESRDIQAA
                     AAQCQALFE"
BASE COUNT          529 a          719 c          849 g          574 t
ORIGIN      
        1 tagccatgat tatgaacgaa tcattggttg atgtgtcgcc gtcgaccgtg atgcgattga
       61 acgagcggtc ggcgatggcc cggatcattt ccttgagcaa cgcgggggcg atgcccgcat
      121 cagtggccag atagcccagc attgtggcca tgtttgggtg gatcatgccg gcgcccttgc
      181 tgatgcccgt gatagtgacc gttttcccgc cgatattgag gcgccgggaa acgattttgg
      241 gttgcgtgtc tgtcgtcatg atgctatgcg cggcggcaaa ccagttggcg gcatgcaggt
      301 ttttgactgc gtcgggcagt gcggaaatca accggtcaac cggcagaggc tccagaatga
      361 cgccagtgga aaaaggcaga acctgtttcg cttgcacatg cagtagttcg gcgagcgccg
      421 cacaggtttg ccgcgcggct ttcagtccgt cggcgcccgt gcccgcgttg gcgttgcccg
      481 tgttgatgac cagtgcccga atctgattgt ctgttgccag atgtgcttcg cacacttgca
      541 ctggcgcagc gcggaagcga ttttgcgtga acacgcccgc caccgttacg cctggcgcta
      601 attgaaagac ggtcagatca cgccggttgg ctttgcggat gcccgcttgt gcgatgccga
      661 tttcgattcc ggcaaccgga aaaatatcag tttcagcggg gacgattaaa ttaacggcca
      721 tgatggacga cttaatgtta agggataaaa cgaagtgtac tttaaccgct accgcgtcga
      781 gcgtctggtt ttcagttttc cgaagacttg ttcagcggca tcgagcgttt gctggataat
      841 ggcgtcatcg tggctggccg aaacgaatcc ggcttcgaat gcggacggcg caaggtatat
      901 tcctttgtcc agcatggcat gaaagaactc tttgaatagc tcaacgtcgc atttggagac
      961 ttcgctgaaa ccggtgggta tggtttcgct gaaataaagc cccaacatgc cgccgacgtg
     1021 atcggtgcaa aatggcacgt ttgccgccac ggcacgctcg cgcaagcctt gggccaactt
     1081 gccggtttga gtgccaagtt tttcgtaaaa ccccggttcg ctcaacagtt tcaatgtggc
     1141 caggcctgcg gcgacagcga ccgggttgcc tgacagagtg ccggcctggt agaccggacc
     1201 cagcggagca ataaagtcca tgatgtcggc gcgcccgccg aaagctccga caggcattcc
     1261 gccgccgatc actttggcca gggtggtgat gtcgggggta atgcccgcca agccctgaac
     1321 gccttgcgga ccgacgcgga aaccggtcat cacttcgtca aagataagca gcgatccgta
     1381 ctgggtgcac aggagacgca agccttccag aaaacctggc actggtttga tcaaattcat
     1441 gttgccgacg atgggctcga cgataatgca ggcgatctcg gagccgtact gtttgaatgc
     1501 ggcttcgacc ccgtcaaggt agttgtaatc cagcaccagt gtgtgctgga taaattcggg
     1561 cgggacgccc gccgaggtcg gctgaccgaa tgtaagcatg cccgaaccgg ctttgaccag
     1621 caggctatcg gcgtgaccgt ggtaacagcc ttcgaatttg atgatttttg gacggcctgt
     1681 gaagccgcgc gccaggcgaa tggcgctcat tgtcgcttcg gtgccggaac tcacaaggcg
     1741 gattttttca atcgacggga ttcgcgcgac gatagtttcg gcgatatcga tttcggcttc
     1801 ggtgggcgcc ccgaaagaca gtccatttac agaggcctcc tggacggcgc gcaccacttc
     1861 gggatgtgcg tggccgagga tggcgggtcc ccaggaaccg acgtaatcaa tgtagcgtac
     1921 cccttcagca tcccacaagt agggcccttg cgcgcgcgtg atgaaacgtg gcgagccgcc
     1981 taccgaacga aaggctctga ccggtgaatt gacgccgcca ggaatggttc ggcaggcgcg
     2041 ttcgaatagt tcggtgttgc gggacataat tttggggctt tgcgtgatgg ttgatggcga
     2101 aagaggctac aggctatagc ataacaagac tggcgttccc gggtcgtgcc gccccagtgc
     2161 tattcgaaaa gggcttggca ttgcgcggcg gcggcctgga tgtcgcggct ctcgaacagc
     2221 gcgctgatca gcgcgatgct atcggcgccg gcttgtatga tcggggcagc attttcaggg
     2281 gttatgccgc cgatggcgat gatggctgcg cgtacaggac tggcgggggt tacagcttgt
     2341 tccgccagca ggcgcgcttg ttgcacgtgt tgcagtgtgg ctcggacggc gtcaggcttg
     2401 atcctggaag gatgcagggc gccgagggcg acgtagtccg cgccgtcggc cagtgcctgg
     2461 cgggccaggg tcagatcgtt gtagcatgag cggccaatga ttttgccaaa gcctaatgcc
     2521 tgtctggctt cggatagcgg gccatcctcg cgacccaggt gaacgccatc ggcgtcaacg
     2581 gcggcggcca tgcgccaatc gtcgttgacg atgaaaacca cgccaagctt tttgcaccgc
     2641 gccgcaagtt cgcgggcttg tggaaggcga c
//
LOCUS       DATCXU010000325         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_132766,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000325 DATCXU010000000
VERSION     DATCXU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132766"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="mnmD"
                     /locus_tag="VIR04_07275"
     CDS             complement(<1..594)
                     /gene="mnmD"
                     /locus_tag="VIR04_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEY9460582.1"
                     /translation="MTGQFAPLVPASVAFDASGIPVSPLYGDVYHSRSGALAQAEHVF
                     LSGNQLPGRWRGRTTFTVCETGFGLGHNFLALWHAWRTDPDRCARLHVVSFEAHPFSR
                     VDLAQLIVPALPDALRALAGQLLTLWPPLLPGLHRLEFESGALTLTLAFGTVKRLATQ
                     ISACVDAYFLDGFAPKKNPEMWTPSLFGQMARMANVGA"
     gene            689..1537
                     /locus_tag="VIR04_07280"
     CDS             689..1537
                     /locus_tag="VIR04_07280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY9460583.1"
                     /translation="MQTTRFHLSENLNAAITAARAAAAILQSHAHNRADLIIDKKTRN
                     DFVSQADRDAEVAIIHLLREHTPEFGIVAEETGGKNSGLATWYIDPLDGTTNFLNGIP
                     HYAVSIALVAHVGAIVIEDHPLERETPVIGVIYDPNREELFTAVHGVGAWLNEHRISC
                     SQARSLSDSVVATGFPFRDFSFADQYMPTLNEAINTTRGVRRFGSAALDLAWTACGRY
                     DGYWEMGLAPWDVAAGTVIVREAGGIAEDLRHIDPWPLKGYVYAGNANTAPALDAMVQ
                     PHLARR"
     gene            complement(1534..>2595)
                     /locus_tag="VIR04_07285"
     CDS             complement(1534..>2595)
                     /locus_tag="VIR04_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9460584.1"
                     /translation="VAVLQYTGGTTGVPKGAMLTHGNLVANVLQVEAVARPALGNLSD
                     RRLTILSALPLYHIFALTVCGLYAAHAGMCSVLVMNPRDLTSIIAAWRRDPIHIFPAV
                     NTLFNALVNRPDFSTLDFSDLLLCFGGGAAVQRGVAEKWQSVTGKPLIEGYGLSETSP
                     VVCVNPTNATQYSGDIGFPVPSTDLVLLDFDERPVPVGERGEVAVRGPQVMKGYWRNE
                     RDTASAFSADGFFKTGDIGIMDARGRLRLVDRKKDMILVSGFNVYPTEVEEVIAAHPG
                     VLECAVVGLPDPHSGEMVKAYVVKKDPTLTEQQLRDWCAQSLTGYKRPHAIEFRSDLP
                     KSNVGKILRRVLRDEANKP"
BASE COUNT          592 a          814 c          706 g          483 t
ORIGIN      
        1 cgccccaaca ttggccatgc gcgccatttg tccaaacagc gaaggcgtcc acatttccgg
       61 gttctttttc ggcgcaaagc cgtccaggaa gtaggcatcc acacaagcgc tgatctgcgt
      121 ggccaggcgc ttgaccgtgc caaaagccag cgtaagcgta agcgcgccag actcgaattc
      181 aagccgatgc aaaccaggca aaagcggcgg ccacaaagtc agaagctgcc cggccagcgc
      241 gcgcagcgca tccggcaacg ccggcacgat caattgggcc aggtcgaccc gggaaaatgg
      301 atgcgcctcg aatgaaacca cgtgcaggcg cgcgcaacga tcagggtcgg tgcgccaggc
      361 atgccacaat gccaggaaat tatgacccag gccgaaacct gtctcgcaga ccgtgaaagt
      421 ggttcggccc cgccagcgcc ccggcaactg attacccgac aaaaaaacat gctcggcctg
      481 tgcgagagcc cctgaccggc tatggtagac gtcgccatag agcggactta caggaattcc
      541 tgaagcatcg aaagcaacgc tggcaggaac caagggtgca aactggccag tcatggttca
      601 cttataaaaa gacaaggggc gccgggacat cgcttctaat gttgcgtaca ctaatagcct
      661 ccctccttac gcgcggcaaa ctgaccctat gcagactact cgattccatc ttagcgagaa
      721 ccttaacgcg gccataaccg ccgcgcgcgc ggcggccgcc atcttgcagt cgcacgccca
      781 taatcgcgcc gatttaatca tcgacaaaaa aaccaggaac gatttcgtgt cccaggccga
      841 tcgtgatgcc gaagtagcta ttatccacct attgcgcgag cacacgcccg aattcggcat
      901 cgtggccgag gaaacgggcg gaaagaattc agggctggcc acctggtata tcgatccgct
      961 ggatggcacc accaattttt taaacggcat cccgcactat gccgtgtcca tcgctcttgt
     1021 cgcgcacgtc ggcgcgatcg ttattgaaga ccatccgctg gaacgcgaaa cgcccgtcat
     1081 cggtgtcatc tacgacccca accgcgaaga actttttact gccgtgcatg gcgtgggagc
     1141 ctggcttaac gaacaccgga tctcgtgctc ccaagccagg tcgctgagcg actcggtggt
     1201 agccaccggg tttcctttca gagacttttc gttcgctgat caatacatgc cgaccctgaa
     1261 cgaagccatc aataccacac gtggagtgcg ccgatttgga tcggctgcgc tcgacttggc
     1321 ctggactgct tgcggccgat acgacggtta ctgggaaatg ggcctggcgc cctgggatgt
     1381 tgctgcgggc acggtcattg tgcgcgaagc aggcggcatt gcggaagacc ttcgccacat
     1441 cgacccctgg ccccttaaag gctatgttta tgccggcaat gcgaacaccg cgcccgcgct
     1501 ggacgcgatg gttcaaccac accttgcgcg gcgctagggc ttattcgcct cgtcacgcaa
     1561 gactcgacgc aagatcttgc cgacattgct tttgggcaaa tcggacctga actcgattgc
     1621 atgcgggcgc ttgtaccccg tcagactctg ggcgcaccag tcgcgcaatt gttgttcggt
     1681 cagggtggga tctttcttga cgacataggc tttcaccatt tcgcccgaat ggggatcggg
     1741 caggcccaca accgcgcatt ccagaacgcc cgggtgggcc gcaatgacct cttccacctc
     1801 ggtcggatac acattgaatc ccgacaccag aatcatgtct ttcttgcgat ccaccaggcg
     1861 caagcggccc cgggcatcca tgattccgat atcgccggtt ttaaaaaagc cgtcagccga
     1921 aaatgcgctg gcggtatcgc gctcgttcct ccagtatcct ttcatgacct gcgggccgcg
     1981 caccgcaacc tcgccgcgct cgcccacagg caccgggcgc tcgtcaaaat cgagcaaaac
     2041 caaatcggtg gacggcaccg gaaagccgat gtcgcccgag tactgcgtgg cattcgttgg
     2101 gtttacacaa acgactggcg acgtttccga cagcccgtaa ccctcgatca aaggcttgcc
     2161 cgtcacactt tgccactttt cagccacccc gcgctgcact gcggcgccgc ctccaaaaca
     2221 caagagcaag tccgaaaaat caagcgtgga aaaatcgggc cgattaacca aggcattgaa
     2281 cagggtattg accgccggaa aaatgtgaat tggatcgcgg cgccacgcag cgataatact
     2341 ggtcaaatcg cgcggattca taaccagaac actgcacatt cccgcatggg cggcgtatag
     2401 cccgcagacc gtcagcgcga agatatgata gagcggcagc gcgcttaaaa tggtcaggcg
     2461 ccggtccgaa agattaccca gcgccggcct tgccaccgcc tcgacttgca gaacattcgc
     2521 caccagattt ccatgcgtca gcatggctcc tttgggcacg ccggttgtgc cgccggtata
     2581 ttgcagcacc gccac
//
LOCUS       DATCXU010000326         6587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_246999,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000326 DATCXU010000000
VERSION     DATCXU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246999"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIR04_07290"
     CDS             <1..214
                     /locus_tag="VIR04_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY9460585.1"
                     /translation="AVDVYEDEPVLNAKNPLVNLPNALCTPHIGFVELNSYEHYYQTA
                     FDNILHFAAGDTTGVLNPEALKHLRP"
     gene            complement(246..818)
                     /locus_tag="VIR04_07295"
     CDS             complement(246..818)
                     /locus_tag="VIR04_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY9460586.1"
                     /translation="MTQGNEHETRAAGVAPETPKHQRAAEASLKMLTRYNAWSDEKIY
                     SALMALPEGEATKERPTRFKTMVNNMNHLYVIQDIFKAHLQGRPHHYTARNTPTHPPL
                     SDLWKMAETMDQWYIDYAAALSSDQLDERVPFQFVGGGDGIMSRREIILHIVNHGSYH
                     RGMVHDVMYQVPAQPPAVDLTVFLRDVAQP"
     gene            1045..2745
                     /locus_tag="VIR04_07300"
     CDS             1045..2745
                     /locus_tag="VIR04_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY9460587.1"
                     /translation="MNRSGIVERFVMGGAVLALLILIVLPLVSLIIGSLYGEGEISGG
                     YFVQVASSRYYLGPLYNSLVLGAWTGLFSVLVGVPLAWAVSRSNTPGKKFIQATVVLS
                     YVSPPFLTAIAFVNLFSPNAGVINVFFRDVLGLDWLTFNIFTMEGLVFVTVLHTFPFV
                     YLLASSALTSVDASYEEAAQILGAGKFRTALSITAPLVAPAVLSGALIAFVTAIALFG
                     SQAIIGLPGRIVTLPTRIYSLFTYPPQYGLASALSLVFIIITVAALYIQRAFLARRSY
                     VTVGGKSSHPRLVELGPVRWLVLAFAALVFIVALVLPYGSLLAVSFSKTWGLNFWKGL
                     TFANYIYVLFEYNITQRAILNSTWLATLTATFAVVLGTLICWLDVRTRIPGRRLLDYT
                     SLIPLGLPGIVMAVALIQFWLAMPVELYGTLTILFLCYVARYLPLGVRAANSSLMQVD
                     VSLEESARILGASWAHTMREITVPLIRPGLFAGWLLVFVPAIHELSASILLFTSDTIT
                     LSVAIFNLEESGFTEPVAALAIVNVVVIGIALWIASRLNSHLASKGPRTGGAVSGVAN
                     "
     gene            2745..3839
                     /locus_tag="VIR04_07305"
     CDS             2745..3839
                     /locus_tag="VIR04_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460588.1"
                     /translation="MSGITIKGLTKSYTEGKGGRAAVNNIDLAIEDHQFVTLLGPSGC
                     GKTTTLRLIAGYLTPDEGTIHLGDREISSPHAVVSPDRRGMGMVFQNYAVWPHKTVRE
                     NVVFGLKVRKVAAARIADQLEKALALVNLTGLEDRFPNELSGGQQQRVALARSLVVEP
                     EILLLDEPLSNLDAKLREHMRMELKQLQRRTGITFVYVTHDQAEALALSDRIAVMHGG
                     VLQQFSAPHEVYTRPANKIVADFMGLVNFLPGTVVADGSGLAVSTFDDVLVSAALSFQ
                     SRAGDQVELAVRPENIQISPVGTGARSVGRIADVIYLGNLVEYMVEVSSGQVLRVQAH
                     PKELFAVGSQVQVTLDADRCNVFAPDAKKA"
     gene            3885..4910
                     /locus_tag="VIR04_07310"
     CDS             3885..4910
                     /locus_tag="VIR04_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460589.1"
                     /translation="MKNGLIFTRCLTGAIALAVTSVAFAAADPVNIEAAQKEGKVAWY
                     SSTPIKRAQQISTDFEKKYGIKVELFRSGGSAIVSRFGQEQAARHKGADVVTASDVTA
                     FEQFVKQGAFIRFNPEGYEHVPASLRSPEGYYVAQRLNVISFYVRTDLVPASDRPKTW
                     ESLLDPKYKGKMVMPNPSFSAQPLSVVGTLASLKGWDYFKKLSANDVMVVKGNQQVVD
                     MVNKGERPIAISADQSYGIVSAAQGHPVEIITPTDGTFIISAPTGVVKGSEHPEAAKL
                     LAQFMITKDVQNYLSSQGDYPSRDDIDPPKGSLRLKDMKIIPIDFNYLKKSSQQIKQT
                     FNMTFLQ"
     gene            4948..5871
                     /locus_tag="VIR04_07315"
     CDS             4948..5871
                     /locus_tag="VIR04_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY9460590.1"
                     /translation="MHDSGGGTRVQSRDHRTEKGGTTLFLREKFTDDGAPVRGIILFV
                     HGSSMASTPPFDLHVPGRPDSSVMEWFARQGFNTWCVDMEGYGRSTKDRRHKSDIDRG
                     AQNLTQAADYIASLRGDVPLLIYGVSSGALRAALFAQRRPDRVRRLALDALVYTGRGS
                     PTLAQRIKKLPQFRASATRRIDRRFVHSIFERDHPGTADPHVIDCFAQAILALDAEIP
                     NGTYVDMCANLPVVDPALITVPTLVMRGQYDGIASLEDLLDFFVKLPNPDKQFTIMPG
                     IAHSSFQQRNYKLVYHILRSFFTQPDPVYAS"
     gene            5955..>6587
                     /locus_tag="VIR04_07320"
     CDS             5955..>6587
                     /locus_tag="VIR04_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY9460591.1"
                     /translation="MKGIEQGQTYPGVEHWTKKGDVNLFLWQKAIPHGTPKGTILFVH
                     GSSMASQPTFDLQVPGRPYSSVMDWFVAQGFNTWCVDMEGYGHSDKNRDMTSNISDGA
                     DDLTAASDYIEAKTGAGPLLFYGISSGALRAAAFAERHPKRIKKLALDAFVWTGKGSP
                     TLAQRSKRLEEFRAMKRRPIDRAFVHSIFNRDHPGTADDSVVEAFADAILA"
BASE COUNT         1320 a         1718 c         1925 g         1624 t
ORIGIN      
        1 ggctgtcgac gtgtacgaag atgaacctgt gctgaatgcg aagaatcccc ttgtgaacct
       61 tccaaacgcg ctatgcacgc cgcatattgg ttttgtcgag ctcaacagct acgagcatta
      121 ttaccagacg gcgttcgaca acattttgca ttttgccgca ggcgatacaa cgggtgttct
      181 gaaccccgaa gcgttgaagc atctgcgtcc atagccaata atgaccgcct gttcgggcct
      241 ggaacctacg gctgcgccac gtcgcgcaga aatacggtca ggtccactgc aggcggttgc
      301 gcgggtacct ggtacatcac atcgtgaacc atgccacgat ggtaggagcc gtggttcacg
      361 atatgaagga ttatctcgcg gcgggacata atgccatctc cgcctccgac gaattgaaag
      421 ggaacacgtt cgtcaagctg atctgaagat agcgcggcgg catagtcgat ataccactga
      481 tccatcgttt ccgccatttt ccatagatca gaaagcgggg ggtgtgtggg tgtgtttcgc
      541 gctgtgtagt gatgcggccg gccctgaaga tgcgccttga atatgtcctg gataacatac
      601 aggtgattca tgttgttgac catcgttttg aagcgcgtcg gacgttcttt tgttgcttcg
      661 ccttccggca aagccataag cgccgagtag attttctcgt cggaccacgc attgtatcgg
      721 gtcagcattt ttagagaagc ttcggccgcc cgctggtgtt tcggggtttc cggcgcgacg
      781 cccgccgccc gtgtttcatg ttcgttccct tgtgtcacga tgcatctcct gtttctgcca
      841 gaggctgatt ccgcagtgtg ctcttgcgca ctccgcactt gtcatattcc gttgtatgta
      901 gttcgttatt gtatgtattt tgtaaactgt gtactataaa taacaggctt acgaacgtaa
      961 gcagagtttg ccgctttgta tttgccggtg cgcacagatt gtggtctgcg ttggcttctt
     1021 tcaaaagagg tgttcttgag cgcgatgaac cgcagcggaa tagtcgagcg ttttgtgatg
     1081 ggtggcgcag tactggcgct gctgatcctg atcgttctgc cgctcgtttc gctgattatc
     1141 ggcagtctct atggcgaggg ggaaataagc ggcggatatt tcgtgcaggt ggcgtccagc
     1201 cgatactatt tgggaccgtt gtacaactcg ctagtgcttg gcgcgtggac agggcttttc
     1261 agtgttctgg tcggggttcc gctggcctgg gcggtcagcc gcagcaacac gccgggcaag
     1321 aaattcatcc aggcgactgt ggttttatct tatgtatcgc cgccttttct gacagccatt
     1381 gcgttcgtca atctgtttag tcccaacgcc ggtgtcatca acgtgttctt tcgcgatgtg
     1441 ctgggtctgg actggctcac cttcaatatt ttcacgatgg aaggcctggt gttcgttacg
     1501 gtgctgcata ccttcccctt tgtctacctg ctggcatcaa gcgcattaac gtcggtggac
     1561 gcctcgtatg aagaggcggc gcaaatcctg ggggcgggaa agtttcgcac cgctctgagc
     1621 atcacggcgc cgctcgtggc tccggccgtc ctgtcgggtg cgctgattgc gtttgtgacg
     1681 gcgattgccc tcttcggttc gcaggcgatc attggtcttc cggggcgtat tgtcacgctg
     1741 ccgacccgaa tttattccct gttcacgtat cccccgcagt atggtctggc ttcggcattg
     1801 tctcttgtct tcattattat taccgttgcg gcgttgtaca ttcagagggc ttttctcgca
     1861 cgccgctcct acgtcacggt gggcggcaaa agttcacacc cacggttggt ggaactgggc
     1921 ccggtgcgct ggcttgtgct ggcgtttgcc gcgctggtat ttattgtggc gctggtgttg
     1981 ccctatggct cgttgctggc cgtgtcgttc agcaagacct ggggcctgaa tttctggaaa
     2041 ggcctgacct ttgcgaatta catttatgtt ttgtttgagt acaacataac gcaacgggcg
     2101 attctgaaca gcacttggct ggcgaccctg accgcaacgt ttgcggttgt gctgggcact
     2161 cttatctgtt ggctggatgt acgaacacgg ataccggggc ggcgtttgct ggactatacc
     2221 tcgttaattc cgctgggcct gccgggcatt gtcatggcgg ttgccctgat ccagttctgg
     2281 ctcgcgatgc cagtggagtt gtatggtacg ttgaccatct tgttcttgtg ttatgtggcg
     2341 cgctacctgc cgctgggtgt ccgggccgcg aatagctcgc tcatgcaagt cgatgtatca
     2401 ctagaggaaa gcgcgcgaat tttgggggct tcctgggcgc acacgatgcg cgaaattacg
     2461 gttccgctga ttcgtccggg cttgttcgca ggttggctac tggtgtttgt gcccgccatc
     2521 cacgaattaa gcgcatccat tcttcttttt acctccgaca ccattacgct gtcggtcgcg
     2581 attttcaatc ttgaagagtc gggctttacc gagcctgtcg cggcgttggc aatcgtcaat
     2641 gtggtggtga tcggaattgc cttgtggatc gccagccggc tgaacagcca cttggcaagc
     2701 aaggggcctc gtactggtgg cgccgtttca ggagtggcaa actgatgtct gggattacga
     2761 tcaagggcct gaccaagagc tatacggaag gaaagggtgg gcgtgccgcg gtaaataaca
     2821 ttgatctggc cattgaagat catcagttcg tcacgctgct gggccccagc gggtgcggta
     2881 aaaccacgac gttaaggttg atcgcgggtt acctcacgcc ggatgagggc acgattcatc
     2941 tgggggacag ggaaatctcc tcgccccacg cggtggtgtc gccggatcgg cgcggaatgg
     3001 gcatggtttt ccagaactac gcggtctggc cgcacaagac ggttcgcgaa aacgtggttt
     3061 ttggcctgaa ggtgcgtaag gtagcggcgg cccggattgc cgatcagttg gagaaggcgc
     3121 tggccctggt caacctgacc ggtcttgaag atcgattccc gaacgagcta agtggcggcc
     3181 agcagcagcg tgtggcgctg gcccgcagtc ttgtggttga gcctgaaatc ctgttgctcg
     3241 atgaaccctt gagcaatctg gatgcgaagc tgcgcgaaca catgcgcatg gaactgaaac
     3301 aactgcagcg tcgcaccggc attaccttcg tttatgtgac gcatgatcag gccgaagcgc
     3361 tggcgctgtc tgaccgcatc gccgtgatgc acggcggggt gttgcaacag ttcagcgcgc
     3421 cgcacgaagt ctatacgcgg ccagccaaca agattgtggc ggatttcatg ggtttggtga
     3481 attttcttcc gggcactgta gtcgctgatg gatccggact ggccgtatcg acctttgacg
     3541 atgtgcttgt cagtgccgcg ctgtcgtttc aatcccgtgc gggcgatcag gtggagcttg
     3601 cagtgcgccc cgagaatatt caaatctcgc cggtcgggac aggggcccga agtgtgggtc
     3661 gaatcgccga cgttatctat ttgggcaatt tggtggagta catggtcgaa gtcagttccg
     3721 gccaggttct gcgggttcag gcgcacccca aagagctttt tgccgtgggt tcccaagtgc
     3781 aggtgactct ggatgcggat cggtgcaacg tgtttgcgcc ggatgcgaaa aaagcctgat
     3841 atttttataa gcgccgtggc atgcatgcgg ctgaggagac attaatgaaa aatggtctaa
     3901 tatttacgcg ttgtcttaca ggagcgattg ctcttgccgt tacctcggtg gcgtttgcag
     3961 cggccgaccc ggtcaacatc gaagcggccc agaaagaagg caaagtcgcg tggtattcgt
     4021 cgacgccgat caagcgtgcg caacagatct ccaccgattt tgaaaaaaaa tacggcatca
     4081 aggtcgagct gttccgctcc ggcggaagtg cgatcgtaag ccgtttcgga caagagcaag
     4141 cggccaggca taaaggcgcc gatgtggtta ccgcgtcgga tgtcaccgcc tttgaacaat
     4201 ttgtgaaaca gggggccttc attcgtttta acccggaagg ctatgagcat gtgccggctt
     4261 cattgcgcag ccccgaaggc tactacgttg cacagcggct gaacgtgatt tctttctacg
     4321 tacgcaccga tctcgtccct gccagcgatc ggcccaagac atgggaatcg ctgcttgacc
     4381 ccaagtataa aggcaagatg gtgatgccca acccttcctt ttcggcacag ccgttgtcgg
     4441 tggtcggaac gttggccagc cttaagggtt gggattattt caagaaatta agtgccaacg
     4501 atgtcatggt cgtcaagggc aatcagcaag tcgttgacat ggtcaataag ggcgagcgcc
     4561 ccattgcgat atccgctgat caatcgtacg gcattgtatc ggctgcccaa gggcatcccg
     4621 tcgagatcat tacgccaacc gacggcacgt tcattatttc ggcgccgacg ggcgttgtca
     4681 aaggctcgga acaccccgaa gcggcaaagc ttctggcgca gttcatgatc accaaggatg
     4741 ttcaaaacta cctgtcgagc cagggtgatt atccctcgcg cgatgacatc gatccgccga
     4801 agggaagctt gcgcttgaag gacatgaaaa taattccgat cgatttcaat taccttaaaa
     4861 aatcgtcgca acagatcaag caaaccttca atatgacatt tcttcagtag cgcgcgattg
     4921 cgggctgtta tgtcggatcg gcaagccatg catgattctg gcggcgggac tcgcgttcaa
     4981 agccgcgatc atcggactga aaagggcgga acgaccttgt ttctgcgcga gaaattcacg
     5041 gatgatggcg cgcctgtgcg cggaattatt ctgtttgtac atgggtcttc aatggcgtcg
     5101 acgccgcctt tcgatttgca tgtccccggc cggcccgatt catcggtgat ggagtggttc
     5161 gcgcgccagg gtttcaacac atggtgcgtc gacatggaag gctatggccg gtctacaaaa
     5221 gaccggaggc acaaatccga cattgaccgc ggagcacaaa acctcaccca ggctgctgat
     5281 tacattgcat cgctgcgcgg cgatgtgccg ttactaatat atggcgtttc ttccggcgcc
     5341 ttgcgcgcgg cgttatttgc gcaacgccgt cccgatcggg tcaggcggct ggcgctcgat
     5401 gcgctggttt acaccgggcg cggcagcccg actctggcgc aacgcatcaa gaagctgcca
     5461 caatttcgcg cctcggcgac acggcggatt gaccggcgtt tcgtgcattc cattttcgag
     5521 cgcgaccatc cgggcacagc cgatccgcac gtcatcgatt gctttgccca ggcgatattg
     5581 gcgctggacg cagagattcc caatggaact tatgtggaca tgtgcgccaa tctgccagtg
     5641 gtcgatccgg cattgataac cgtacctaca ttggttatgc gtggtcagta tgacggcatc
     5701 gcttcactgg aggacttgct ggatttcttt gtgaaactgc ctaatcccga caagcaattc
     5761 accatcatgc cgggaattgc ccactccagt tttcagcaga gaaactacaa acttgtgtac
     5821 catattttgc gatctttttt tactcaaccc gatcctgttt atgcaagttg acctggcacc
     5881 gccaaaagac ggacgcatgt cagcacaggc gagggttgcg ataggctaac gacatttaca
     5941 gcggacgagg aatcatgaag ggaatcgagc aaggacaaac ctacccgggc gttgagcact
     6001 ggacgaagaa gggcgatgtc aatctttttt tgtggcaaaa ggctattccg catgggactc
     6061 ccaagggaac gatccttttt gtgcatggct cgtcgatggc ttcccagccg acctttgatc
     6121 ttcaagttcc ggggcgacct tattcgtcgg tcatggactg gttcgtggcg caaggcttca
     6181 atacatggtg cgtcgacatg gagggctatg gtcattcgga caaaaatcgc gatatgacct
     6241 cgaatatttc tgacggcgcc gacgacctga cggccgcctc cgactatatc gaagcaaaga
     6301 cgggggcggg acctttgctg ttttatggga tctcgtccgg cgcgctacgc gcggcggctt
     6361 tcgcggaacg tcacccaaaa aggataaaaa aattggcgct ggatgccttt gtctggaccg
     6421 gcaagggtag tcccacgctt gcccagcgca gcaagcgtct ggaagaattt cgggccatga
     6481 aacgcaggcc tattgacagg gcctttgtcc attccatttt taaccgcgac catcccggca
     6541 ctgccgatga ttcggtggtg gaggctttcg ccgatgcgat cctggct
//
LOCUS       DATCXU010000327         6851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_675926,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000327 DATCXU010000000
VERSION     DATCXU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675926"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VIR04_07325"
     CDS             complement(<1..180)
                     /locus_tag="VIR04_07325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY9460592.1"
                     /translation="MDLRKLKTLIDLVSDSGIAELEITEGEGKVRIVKFSQSAQPVAP
                     VVQAPVVAAASAAPAA"
     gene            complement(276..710)
                     /gene="aroQ"
                     /locus_tag="VIR04_07330"
     CDS             complement(276..710)
                     /gene="aroQ"
                     /locus_tag="VIR04_07330"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY9460593.1"
                     /translation="MAQHILVLHGPNLNLLGNREPEIYGRQTLADINAGLRKIAAQSG
                     ADCDDFQSNREGELVDRIQAARQDGTDFIIINAAAYTHTSVAIRDALAAVAVPFIEVH
                     LSNVHKREPFRHHSYLSDSAQAVIVGLGAFGYEAALRYALAN"
     gene            complement(787..1299)
                     /locus_tag="VIR04_07335"
     CDS             complement(787..1299)
                     /locus_tag="VIR04_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY9460594.1"
                     /translation="MKRRDFLRHSAIWAVTAAAVNWRDAARPVWAAQADPVFDQAFPG
                     LDGKPFALADCLGKPVVLNFWASWCPPCVKEMPDLETLHKKYPAVHFLGLAIDSASNV
                     KTFIRKVPITYTLLLAGHDGIPLMQTLGNKVGGLPFTVVFDGKGKIENRVLGQVKPAV
                     LERVLLSIMS"
     gene            1403..2803
                     /gene="mpl"
                     /locus_tag="VIR04_07340"
     CDS             1403..2803
                     /gene="mpl"
                     /locus_tag="VIR04_07340"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY9460595.1"
                     /translation="MQLHILGICGTFMGGLALIARAAGHTVTGCDAGVYPPMSTQLEE
                     QGIRLIEGFDTDQLELKPDLFIIGNVITRGNPLMEAILDRGLPYTSGPQWLGENILQT
                     QRVLAVAGTHGKTTTSSMLAWILDYAGKTPNFLIGGVAADLKVSARYRAEQNLFVIEA
                     DEYDTAFFDKRSKFVHYHARTVILNNLEYDHADIFPDLKAIETQFHHLVRTIPGSGHI
                     IRPADSAALDRVLALGCWSSIETFGTRGQWQAIENGQAGHIDIVHNSVHAGTIIWQLT
                     GRHNSMNALAALAAAERAGVPVDQGIAALSAFRGVKRRMELRGTVNQIKVYDDFAHHP
                     TAIATTIDGLRRKIGNGRILAVIEPRSNTMRLGAMAAQLPDALKQADHVFCFGAISGK
                     YALGWSPQQVLAPLGARALSFDNLDKMVDAISAMALPGDSILVMSNGSFGGIHQRLLE
                     RLQSLNRSQSLAQASP"
     gene            2800..3393
                     /locus_tag="VIR04_07345"
     CDS             2800..3393
                     /locus_tag="VIR04_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY9460596.1"
                     /translation="MILYLHGFRSSPQSLKATLMARAMRERGMAHDWFCPQLPASPKE
                     ALALAHRSIEQAQAEAGWQADRDLTIIGSSLGGYYATCLAEHWGCRAALLNPVVHAAR
                     DLATQVGSHTQFYSGQPFNFLPEYIDELADMAVNKPRHPERYFLLAATGDELLDWREM
                     ADWFAGCRQQIIEGSDHGISDFAHWLPQVMEFALVRA"
     gene            3440..5326
                     /locus_tag="VIR04_07350"
     CDS             3440..5326
                     /locus_tag="VIR04_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY9460597.1"
                     /translation="MYVLYEDSGNFKAEKIFSQSDATMQVESESGKRSKIKNASTLFS
                     FDQPGPVELLEQARALASGFEIDFLWECAPSEDFDAAEFANEYFGHKPSAVEKAALIF
                     ALNSAPAYFHRRGKGHYKPAPPDILAAALASIEKKQKQAAQQQAWTNAMIAGELPQPI
                     AEVADTLLTNPDKNSLQWKAFDAAVIRLNVSPEQLLLKLGAWPNSLALHRHRFFSTHF
                     PKGTKFGPVDVPQVGADLPLADVPAYSVDDISTTEIDDALSVVLIDKDTVQIGVHIAV
                     PGLVVMRDNALDKLARARMSTVYMPGQKIPMLPDEVIHAFSLDAGQLRPVLSLYVRSN
                     AASGEILSSETRLERIQVRENLRHNLLDAQISEEALNDPDAQLPYADWLRPLWRFAQQ
                     LSAQRDIVRGKPESNNRVEYSFYLDGPADNPDSIIRLEARQRNAPLDRLVAEYMILAN
                     SQWGGLLAQHGVPGIYRSQQAGRVRMSTQALPHEAIGVAQYAWTTSPLRRYVDLVNQG
                     QLLAAVQHGVSARLVAPFKPKEADLFAIIGAFDSQYALWSEFQNTMERYWCIRWLQQQ
                     GLTTVNASVLRDNLVRLSCAPLITRIAGLPELERGQIISLEIMSYDDLALELDCRYLE
                     TIAA"
     gene            5360..6247
                     /locus_tag="VIR04_07355"
     CDS             5360..6247
                     /locus_tag="VIR04_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY9460598.1"
                     /translation="MPNTLSTALLSPPSGPRNDHLRIAIVLSLCVHAGVLAIHFAKPA
                     PTVAKERTLEVTLVNARTESAPIKPKLLAQNQINGGGETKTGAASSPLPRTSSVPADQ
                     VILEALRKRQAELEEEQKQLYTQLESRQKTLLAHKRPDLLQQSDDPGTDNRNQESLIL
                     NAQISAIKERIERYNALPRQQFSGPSAQAADYAEYVEAWRKKIEMLGTENYPAEARGK
                     IYGKLQLTVYIKKNGDLARIEINRPSSHAILNLAAQRIVQLAAPFAPLPPSLAEHTDV
                     FAITRTWSFTANQLNTETP"
     gene            6244..>6851
                     /gene="aroE"
                     /locus_tag="VIR04_07360"
     CDS             6244..>6851
                     /gene="aroE"
                     /locus_tag="VIR04_07360"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY9460599.1"
                     /translation="MTAPTIRLRYAVIGNPIEHSRSPFIHEQFARQLGIALDYEKIKA
                     PVDGFAATVGQFFSGGGAGLNVTLPFKEQAAVLARRLGKRARLANAVNTLWMEQGLLH
                     GCNTDGVGLLNDLQRLGLALQDKRILLVGAGGAAKGIILPLLDAGCARLRVINRTPAR
                     ALELQARIVQQRPELARLLSAGGLNDANGQWDLVVNATSSSMG"
BASE COUNT         1573 a         2035 c         1824 g         1419 t
ORIGIN      
        1 tgcggcgggc gcggcgcttg cggcggcaac aacaggcgct tgcaccacgg gcgcaacggg
       61 ttgcgccgac tgagagaatt taacaatcct gactttgccc tctccctcgg tgatttcgag
      121 ctcggcaata cccgagtctg acaccaagtc gatcaaggtc tttaattttc gaaggtccat
      181 acagatttcc catggcattg cgctgcaagc caacgtgcgc agcgaacgct aaattgttaa
      241 aaaacgccga ggagacgccg cgatgcttca gttacttaat tagccaaggc gtagcgcaag
      301 gcggcttcgt atccaaacgc gcccagcccc actatcaccg cctgagccga gtcggacaaa
      361 tacgaatgat ggcggaaagg ctcccgcttg tgtacgtttg ataaatgcac ttcaatgaac
      421 ggaacggcga cggcggccaa ggcgtctcgg atggccacac tggtatgcgt ataagcagcc
      481 gcgttaataa taatgaagtc agtgccgtcc tgtcgcgccg cctgaatccg gtcgacaagc
      541 tcgccttctc ggttgctttg aaagtcgtcg caatctgcac cgctttgcgc cgctatctta
      601 cgtaaacctg cgttaatgtc ggccaaagtc tgtcgcccat agatctcggg ctcgcgattg
      661 ccgagcaaat tcaagttagg accgtgcaat accagtatat gttgcgccat tacagaataa
      721 ttttacctac agaacgcctt tttacgctaa aaagctgcat ttgtccatca agtgggcagc
      781 atttggctac gacattatac tgagcaacac tcgttcaagc accgctggct tgacctggcc
      841 cagtacccga ttttcaattt tgccttttcc gtcgaagaca accgtgaaag gcaggccccc
      901 aaccttgttt cccaaggttt gcataagcgg aatgccgtcg tggccggcaa gcagcaaggt
      961 ataggtgatg ggtaccttcc ggataaacgt ttttacgttg ctggcactgt cgatcgcaag
     1021 ccccagaaaa tgcaccgccg ggtatttttt atgcaatgtt tcgaggtcgg gcatttcctt
     1081 gacgcaagga gggcaccagc ttgcccagaa attcagcacc acgggtttcc ccaggcagtc
     1141 ggccagtgca aatggtttgc catccagacc cggaaaagcc tgatcaaata cgggatcggc
     1201 ctgagcagcc cataccggcc gggccgcatc gcgccaatta accgcggcgg cggtcaccgc
     1261 ccatatcgcc gaatggcgca agaaatcccg ccgtttcatt tttacctcgc acaggcaaac
     1321 accgctgttt aaacatggca gaaaaggctt catctgatag atgagcccct cttctgacta
     1381 caatatcaat cggaggatca ttatgcaact acatatactc gggatctgcg gcacgttcat
     1441 gggcgggctc gcccttatcg cgcgcgcagc gggtcatacg gtgactggtt gcgatgccgg
     1501 cgtctatccg cccatgagca cccaactgga agaacagggt atcaggctga tcgaaggatt
     1561 cgataccgat caacttgaat taaagcccga tctgttcatt atcggcaacg tgataacccg
     1621 cggcaaccct cttatggaag ccatcctcga tcggggactg ccctacactt ccggccccca
     1681 gtggctgggc gaaaacattt tacagacgca gcgcgtactg gcggttgccg gaacgcacgg
     1741 caaaaccaca accagttcca tgctagcctg gattctggac tacgcgggca aaacgcccaa
     1801 ttttttaatc ggcggcgttg ccgccgacct gaaagtgtct gcccgctacc gcgccgaaca
     1861 gaatttgttt gtcatcgagg ccgatgaata cgacaccgct tttttcgata aacgatcgaa
     1921 attcgttcat tatcatgcgc gcaccgtcat attgaataac ctcgaatatg atcacgccga
     1981 cattttcccc gatcttaaag ccatcgagac ccaattccat cacctggtgc gaaccattcc
     2041 cggatcggga cacattattc gccctgccga ctcggccgct ctggaccggg ttctggccct
     2101 tggttgctgg agctcgattg aaacgtttgg cacgcgcgga cagtggcagg ctattgaaaa
     2161 tggacaggcc ggccatatcg acatcgttca caattccgtt catgccggca cgatcatctg
     2221 gcaacttacc gggcgccata acagcatgaa cgcactggct gcgttggcag ccgctgaacg
     2281 cgcaggcgta ccggtggacc agggcattgc cgcgctttca gcgtttcgcg gcgtgaagcg
     2341 gcgcatggaa ctgcgcggaa ccgtcaatca aatcaaggtt tacgacgact tcgctcatca
     2401 cccgaccgct atcgcaacca cgatcgatgg cttacgccga aaaattggca acgggcgcat
     2461 actggccgtc atcgagcccc gttccaacac aatgcggcta ggcgcgatgg cggctcaact
     2521 tcccgacgcg ttaaagcagg ccgatcatgt attttgcttc ggcgcaatca gcggcaaata
     2581 tgcgctcggc tggagtcccc aacaggtgct ggcgcccttg ggcgcgcgcg cgttgtcctt
     2641 tgacaatctg gataaaatgg ttgacgcgat cagcgcgatg gcgctgccag gcgattcgat
     2701 tcttgtcatg agcaacggca gcttcggcgg cattcaccaa aggctgctgg aacgtttaca
     2761 gagcctgaac cgttcgcaaa gcctcgcgca ggcaagccca tgattctgta cttgcatggt
     2821 tttcgctcgt cgccccagtc gctgaaggca accctgatgg cgcgcgccat gcgggaacgc
     2881 ggcatggcgc acgactggtt ttgcccacag ttacccgcca gtccaaaaga agcgctggcg
     2941 cttgcccatc ggtcgattga acaagcccaa gccgaagccg ggtggcaagc ggatcgggat
     3001 ctgaccatta tagggtcgtc tttaggcggg tattacgcga cctgcctggc cgagcactgg
     3061 ggatgccgcg ccgctttact gaacccagtg gttcatgcgg cccgcgatct ggctacccag
     3121 gtgggcagcc acactcagtt ttacagcggc cagcctttta attttttgcc ggaatatatc
     3181 gacgaactgg ctgacatggc agttaataaa cccaggcatc cggaacgcta ctttttactt
     3241 gcagcgactg gagacgaatt gctcgattgg cgcgaaatgg ctgactggtt cgccggttgc
     3301 cggcaacaga ttattgaagg gagcgaccat gggatctcgg acttcgcaca ctggctgccg
     3361 caggtcatgg aatttgccct tgttcgcgcc tgaccttttt ttaccccaca cgcacaccgg
     3421 cgtgctttga cggatgacta tgtacgttct ttatgaagac agtggaaatt tcaaagcgga
     3481 aaaaatattt tcgcaaagcg atgcaaccat gcaggttgaa tccgagtctg gtaaacgcag
     3541 caaaataaaa aatgccagca cactgttcag cttcgatcaa cccggcccgg tcgagctgct
     3601 ggagcaggcg cgggcgctcg cttcaggctt cgagatcgac tttttatggg agtgcgcgcc
     3661 cagtgaagat ttcgacgcag ccgagtttgc caatgaatat ttcggacaca agcccagtgc
     3721 ggttgaaaaa gcggcattga ttttcgccct taacagtgcg cctgcttact tccaccggcg
     3781 cggcaaagga cattacaagc ccgctccgcc cgacatcctt gcagccgcct tggcttcaat
     3841 cgaaaaaaag cagaaacagg ccgcccagca acaggcctgg acaaacgcga tgatcgctgg
     3901 cgaattgccg cagcccattg ccgaagtagc ggacacactg ctgacgaatc ccgataaaaa
     3961 ctcgctgcaa tggaaggcgt ttgacgccgc cgtgatccgg ttaaacgtca gcccggaaca
     4021 attgctgctc aagctaggcg catggccgaa ctccctggcc ctgcatcggc accgcttttt
     4081 ttccacccac ttcccgaagg gcacgaagtt cggccctgtc gatgtccccc aggtcggcgc
     4141 ggatctgccg ttggcggacg taccggccta ttcagtcgac gacatcagta caactgaaat
     4201 cgacgacgcc ctgtcggtcg tcctgatcga caaagacacc gtgcaaattg gggtgcatat
     4261 tgcggttcca ggccttgtcg tcatgcgcga caacgccctc gacaagctgg cccgcgcccg
     4321 tatgtcaacc gtctacatgc cggggcagaa aattcccatg ctgcccgacg aggttattca
     4381 tgcattctca ctcgacgccg gccagttgcg acccgtgctt tctttatacg ttcgctccaa
     4441 cgccgcttcc ggagaaatac tgtcgtccga aacccggctt gaacgcatac aggtgcggga
     4501 gaacctgcgc cataatctgc tggatgcgca aatcagcgag gaagctctga acgaccccga
     4561 tgcgcaactg ccgtacgccg actggctgcg gcccctgtgg cgatttgcgc aacaattgag
     4621 cgcgcagcgc gatatcgtac gcggcaagcc cgagagcaac aatcgcgtcg agtactcgtt
     4681 ttatctggat gggcccgccg acaaccccga ctcgatcatt cgcctggaag cgcgtcagcg
     4741 caatgctccg ctggatcgcc tggtggcgga atacatgatt ctggccaata gccagtgggg
     4801 cggcctgctg gcgcaacacg gcgtgccggg catttaccgc tcgcagcagg ccggacgagt
     4861 gcgcatgtcc acccaggcct tgcctcatga agccattggc gtcgcccaat atgcctggac
     4921 cacatctcct ttgcgccgtt atgtcgatct cgtcaatcag gggcaattgc tggccgccgt
     4981 ccaacatggc gtttcggcac gcctggtggc gccatttaaa ccgaaagaag ctgatctgtt
     5041 tgcaattatt ggggctttcg attcgcaata tgcgctgtgg tccgaattcc agaacaccat
     5101 ggaacgctat tggtgcatac gctggctgca acagcaaggt ctgaccaccg taaacgccag
     5161 cgtccttcgc gataatctgg tgcgcttatc gtgcgcgccc cttatcaccc gtattgcggg
     5221 cttgcccgaa ttggaacgcg ggcaaataat ctctcttgag atcatgagtt acgatgatct
     5281 tgcgcttgag ctggattgcc gctaccttga aaccatcgcc gcgtagccta ctttacttcc
     5341 caacgcgaag accgaaaacg tgccgaacac tttatccacc gcgctgttgt cgccaccgtc
     5401 cgggccgcgc aacgatcatc tacgcatagc gatagttctg tcgctgtgcg tacatgcggg
     5461 cgtgctggcc attcacttcg caaagccggc tccaacggtg gcaaaagaaa gaactctcga
     5521 agtcacgttg gtgaatgccc gcaccgaatc agcccccatc aagccgaaat tgctggccca
     5581 aaaccagata aacgggggtg gagaaaccaa aaccggcgcc gcctcctcgc cattgccccg
     5641 tacaagctct gtcccggccg accaagttat ccttgaggca ctgcgcaaac ggcaagcgga
     5701 actcgaagag gagcaaaagc agctttacac ccaactggag tcccggcaaa aaaccctgct
     5761 tgcacacaaa cgtccggatt tgctgcagca atcggacgac cccggcaccg ataaccgcaa
     5821 ccaggaaagc cttatcctga atgcgcaaat cagcgccatc aaagagcgca tagagcgcta
     5881 taacgcactg ccaagacagc agttttcggg cccatcagcc caggcggcag actatgctga
     5941 gtatgtcgag gcgtggagaa aaaaaatcga gatgttgggc accgaaaact acccagccga
     6001 agcgcgcgga aaaatctacg gcaaactgca actgactgtc tatataaaaa agaatggcga
     6061 cctggcccgg atcgagatca accggccgtc cagtcacgcc atactcaatc tggccgctca
     6121 gcgaattgtg caattggcgg ccccttttgc accactgccg ccatccctgg ccgaacacac
     6181 ggacgtattc gccatcactc gcacgtggag cttcaccgcc aaccagctta ataccgagac
     6241 accgtgaccg ccccaacaat ccgcctgcgc tatgccgtca tcggcaatcc gatcgaacac
     6301 agccgatcgc cattcatcca tgaacaattt gcccgtcagt tgggcatcgc gctggattac
     6361 gaaaaaatta aagccccggt tgacggcttt gccgccacag tcggacaatt cttttccgga
     6421 ggaggggccg gtctgaatgt gacgctccct tttaaagaac aagctgctgt cctggctcgt
     6481 cgccttggca aacgcgcccg gctggcgaac gcggtcaata ccttgtggat ggagcaaggt
     6541 cttttacatg gctgcaatac cgacggcgtc ggcttgttga acgacctgca acgccttggg
     6601 ctcgcgctgc aagacaaaag aatattgctg gtaggggccg gaggtgcggc gaaaggaata
     6661 atccttccgt tgcttgatgc gggctgcgcc cggctgcgcg tcataaaccg aacgccggcg
     6721 cgggcgcttg aactgcaagc gcgcatcgtc caacaacggc ccgaacttgc tcgcttgcta
     6781 tcggccggcg gtctgaacga cgcaaacgga caatgggatc ttgtcgttaa cgctacatcc
     6841 agcagcatgg g
//
LOCUS       DATCXU010000328         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1102167,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000328 DATCXU010000000
VERSION     DATCXU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102167"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VIR04_07365"
     CDS             complement(<1..140)
                     /locus_tag="VIR04_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEY9460600.1"
                     /translation="MNLANLGLTGMAAAQIRLQTAGHNINNAATDGYNRQTVLVSSAG
                     AV"
     gene            complement(254..1219)
                     /gene="flgJ"
                     /locus_tag="VIR04_07370"
     CDS             complement(254..1219)
                     /gene="flgJ"
                     /locus_tag="VIR04_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEY9460601.1"
                     /translation="MSFTQYIPRPVNDASAFDPGQINLLKRQVQDGGSADANKKVARQ
                     FEAMFIQMLLKQSHQSSASSGPFDSDQTRMAQSMGDEQMALQLSDPGIGLAQALLDQI
                     GARQPDNGTAQPSSSTPPELSTSRVPGLRSRVGQDATHVASSISALIDLLSQQAPAGL
                     ASAIKGAPEHIRSFVSAMSGAAKAAANESGVPLKLILSQAALESGWGKREIQGADGSP
                     SYNLFGIKASPGWKGKVVNVLTTEYDSGVARKVVQPFRAYSSYAESFADYARLIGNSA
                     RYEKVVSAPNAEIAAQKIQDAGYATDPGYAQKLISIMGYFDTGAI"
     gene            complement(1231..2388)
                     /locus_tag="VIR04_07375"
     CDS             complement(1231..2388)
                     /locus_tag="VIR04_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY9460602.1"
                     /translation="MTPILNFALSDCSRLWKRGAALVAALVAALLLSLSVAHAERIKD
                     LASIQGVRANQLIGYGLVVGLDGSGDQVRQTPFTQQSLTNMLSQLGVTVPQGTNMQVK
                     NVAAAMVTARLPAFAQPGQSVDVVVSSMGNAKSLRGGTLLMTPLKGANGQVYAMAQGN
                     LLVGGAGAQAGGSSVQVNQLNGGIITNGAIVERSVPTTFARDGMINLEMNTTDFGVTQ
                     NVAAALNRAFGSGTARALDGRVVQIKGPVNASSQAAFLSQVENVQVTLPPARAKVIIN
                     ARTGSVVMNRTVTIDEAAITYGNLSVVISQQSQVSQPNTPFAGGQTVVTPSTQIEMRS
                     DQGSLKRVRTSANLADVIKALNALGASPQDLLSILQTLKSAGALRADLEII"
     gene            complement(2390..>2944)
                     /locus_tag="VIR04_07380"
     CDS             complement(2390..>2944)
                     /locus_tag="VIR04_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY9460603.1"
                     /translation="PEANGSIYQPTAYGNYPLFEDRRPRNVGDIVTIVIQEKTNAAKN
                     VATATDRSGSATLGMTALPSVLPSELGAKQNFDASGANKASGKGSSSADNTFSGTLTT
                     TVVGVLSNGNLQIAGEKQIAINRGSEYIRFSGVVDPRSITGSNTVSSTQVADARIEYR
                     SKGTMDEVQTMGWLQRFFLNISPF"
BASE COUNT          610 a          888 c          809 g          637 t
ORIGIN      
        1 ttaacggccc ctgcgctgga caccagcacg gtttggcgat tgtagccgtc cgtcgctgcg
       61 ttattgatat tgtggccggc cgtttgcaag cgtatctggg ccgctgccat accggtaaga
      121 ccaaggttgg ccagattcat aagggtttcc gttcctaatg tttgggagca agctccgcat
      181 atgcgggacg actgcctgat gcatttacgg cagcgggccg aaaatattta attggccggc
      241 tcaaagacga gattcaaatt gcgccggtgt caaaataccc catgatcgat atcagctttt
      301 gcgcatagcc gggatcagtg gcatagccgg cgtcctgtat cttctgcgca gcaatctccg
      361 cgtttggcgc gctaacaacc ttctcatagc gcgcactatt tccaatcagc cgggcataat
      421 cggcgaacga ttcggcgtac gaagaatagg cgcgaaacgg ctgtactact ttcctggcca
      481 cgcccgagtc gtattcggtc gtcagcacat tgaccacctt gcccttccag cccggactgg
      541 ctttgatgcc aaacaagttg tagctggggc tgccgtccgc accctgaatc tcgcgcttgc
      601 cccagcccga ctccagcgcc gcctggctta aaatcagttt cagcggcacg ccgctttcat
      661 tggccgccgc cttggccgcg ccagacatgg ccgacacaaa gctgcgtata tgctcgggcg
      721 cccctttgat ggccgacgct agtccagccg gcgcctgctg gcttaacaaa tcgatcaagg
      781 ccgaaatgga cgaggccaca tgggtcgcat cctggcccac ccgcgaacgc aagccaggca
      841 cgcgcgacgt tgacaattca ggcggggtgg aggacgaagg ttgcgccgtg ccgttgtccg
      901 gttgccgcgc gccgatttga tcaagcaaag cctgcgccag accgatgcca gggtcagaca
      961 gttgcaacgc catctgttcg tcgcccatgc tttgggccat gcgcgtctga tcgctatcga
     1021 acggccccga cgaggcactg gactgatgtg attgtttcaa aagcatctgg atgaacatgg
     1081 cctcgaactg gcgcgccact ttcttgttgg catccgcgct gccaccgtcc tgcacctgac
     1141 gtttaagaag attaatctgt cccggatcaa atgccgaggc gtcgttgaca ggccggggta
     1201 tgtattgagt aaatgacaca ggctcgcgtc ctatatgatt tccagatcgg ctcgcaaagc
     1261 gccggcgctt ttcagagttt gcaaaatcga caacaagtcc tgtggcgacg cccccaacgc
     1321 attaagcgcc ttgatcacat cggccagatt ggcgctggtg cgcacgcgct taagactacc
     1381 ctgatcggaa cgcatctcga tttgcgtgct gggcgtcacc acggtttgtc caccggcgaa
     1441 aggcgtattg ggctgactga cttgcgactg ctggctgatc accaccgata aatttccata
     1501 tgtgatcgcg gcctcgtcaa tggtcacagt ccgattcatg acgaccgaac ctgtgcgcgc
     1561 gttgatgatc accttggcgc gcgccggcgg cagtgttact tgcacgtttt caacctggga
     1621 cagaaaagcc gcctggctgg acgcattgac cggcccctta atctgcacca cccggccatc
     1681 gagagcccgg gctgttccac ttccaaaagc gcggttcaaa gcggcggcca cgttttgcgt
     1741 cacgccgaaa tccgtcgtgt tcatctcaag attgatcatg ccatcgcgcg cgaatgtggt
     1801 gggtacgcta cgttcgacga tggcgccatt ggtgatgatg ccgccgttca actgattgac
     1861 ttgcacactg gagccgcccg cctgcgctcc ggccccgccc accagcagat tgccttgcgc
     1921 catggcgtaa acctgcccgt ttgccccctt taaaggggtc atcagcaagg tgcctccacg
     1981 cagacttttg gcgttaccca tggaagacac caccacatcc acgctttgac cgggctgagc
     2041 aaaagccggc aggcgcgccg tcaccatcgc cgccgccacg tttttaacct gcatgttggt
     2101 gccttgcgga acagtgacgc ccaattgcga cagcatgttc gtcaggcttt gctgcgtaaa
     2161 cggtgtctgg cgaacctgat cgccgctgcc atcgaggcca accactaaac cgtaaccaat
     2221 caattgattg gcccgcacgc cctgaatcga cgccagatcc ttgattcgtt cggcgtgcgc
     2281 gacagacaac gacaacaaca gcgccgcaac cagcgccgca accagcgcag caccacgttt
     2341 ccagaggcga gaacagtcag agagcgcaaa attcaatatt ggcgtcatgt cagaacggtg
     2401 aaatattcag gaaaaaacgt tggagccagc ccatggtttg aacctcgtcc atcgtgccct
     2461 tgctgcggta ttcaatacgc gcatcagcga cttgtgtcga agaaaccgtg ttggacccag
     2521 taatggaccg aggatccacc acgcctgaaa aacggatata ttcactaccc cggttgatcg
     2581 caatctgctt ttcgccggca atttgcaaat tgccgttgga caacactccc acaaccgttg
     2641 ttgtgagggt gcctgagaac gtattgtcgg cgctgcttga tcccttgccc gacgctttat
     2701 tggcgcccga cgcatcaaag ttctgtttgg ccccgagttc gcttggcagg acactgggca
     2761 acgcggtcat gccaagagtc gcgctgccgg agcgatcggt tgccgtcgcc acattcttgg
     2821 cggcattcgt cttttcctgg atgacgatcg tgacgatatc acccacattg cgcgggcgcc
     2881 tgtcttcgaa caacgggtaa ttgccgtagg cggtgggctg gtagatagaa ccattggctt
     2941 cggg
//
LOCUS       DATCXU010000329         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_879043,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000329 DATCXU010000000
VERSION     DATCXU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879043"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VIR04_07385"
     CDS             complement(<1..274)
                     /locus_tag="VIR04_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY9460604.1"
                     /translation="MAENEAETSGTEFIEGVKASLDDAEKLLREAAETTGDKAAELRE
                     SAMRSLRRTREALYDTQDALLAQGRKAARVTDDYVHDNPWQAIGLAG"
     gene            complement(429..1850)
                     /gene="dacB"
                     /locus_tag="VIR04_07390"
     CDS             complement(429..1850)
                     /gene="dacB"
                     /locus_tag="VIR04_07390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY9460605.1"
                     /translation="MKRGGRMLAAMSLACLFAANALAQGLPTDLAKSWRAAALPESAL
                     SLVVQEINGPRLVAINPSTPRNPASVMKMVTTWSALSGLGPDYVWRTTFLANNGAQID
                     AQGSLRGPLYLKAGGDPFLTVEGLWSLLRELRLRGIKNLSEVIVDRSIFGNVATDPGQ
                     FDGAPDRPYNASPDAMMVNLGAVRLLFQPDARAKKWIPVIDPPFFGIRVIGDVKWSSA
                     VCPGAPSVATAMSSQGNNVTIHVSGTAAGACGEFSVYRLALSQPEYFSALFRLLWKES
                     GGTLGKGVRAGLVPASAKPIVWHDSENLAELIRVINKHSNNVMARTLSLTLGAETSGP
                     GATPESGARAILEVLKGQNIDTRGWVIDNGAGLSRDARLTAQGLSEMLGAAWRSPLMP
                     EFISSLAISGVDGTVRRRLRNGEAKGMAHLKTGTLRDVRALAGYVLGASGKRYIVVSI
                     ANDENSGKVRAFDDALITWLAER"
     gene            2044..3078
                     /gene="queA"
                     /locus_tag="VIR04_07395"
     CDS             2044..3078
                     /gene="queA"
                     /locus_tag="VIR04_07395"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY9460606.1"
                     /translation="MNHSLQLSQFDYVLPPELIAQTPTPERTGSRLLNLDADGALHDY
                     QFSDLPRLLKPNDLLVFNDTRVIKARLRGQKESGGKIEILIERIIRENGALAHIKASK
                     SPKAGSALIIDEDFSLNVLGRQGELFELEFPGRVLDYLERYGSTPLPPYIEHAAGQED
                     EERYQTVYARNPGAVAAPTAGLHFDQALLAQLTKQGIRQTFVTLHVGAGTFQPVRVQD
                     LSQHIMHSEHYTVSEETLAQVRATHDSGGRVIAVGTTSVRALEAAATRASQSGGIEDD
                     TRLFITPGYKFHFIDALITNFHLPQSTLLMLVSALAGMEPIRRAYEHAVAARYRFFSY
                     GDAMFIESPK"
BASE COUNT          709 a          968 c          805 g          646 t
ORIGIN      
        1 ccccagccag gcctatagcc tgccacggat tgtcgtgcac gtagtcatct gtcacgcgcg
       61 ccgccttgcg cccctgggct aatagagcgt cttgcgtgtc gtacagcgcc tcgcgtgtgc
      121 gtcgcagcga tcgcattgcg ctttcacgca attccgccgc tttatcgccg gttgtttcgg
      181 ctgcttcgcg cagaagcttc tcggcgtcgt ccaagcttgc tttcacgcct tcgatgaatt
      241 ccgtgccgct ggtttcagct tcattttccg ccatgatccg ctccgaacta aaacgtgaac
      301 cccatgctac cttatgtgca caccgccgca acgacaaaag atgaccagac gttaacagat
      361 atgagcggcg atcaacgcat tgattttctc agaaataaaa accactttca atatacttca
      421 cgattgaatt agcgttcggc cagccatgtg atcaacgcgt catcaaaggc ccgcaccttg
      481 cccgaatttt catcatttgc aatactgacc acaatgtagc gtttgccgct agcgcccaaa
      541 acataaccgg ccagcgcgcg cacgtcgcgc aatgttccgg ttttcaaatg cgccattcct
      601 ttggcttcgc cattgcgcag gcgccgcctc accgtcccgt ccacacccga aatcgccagc
      661 gatgagataa attcgggcat cagcggcgac cgccacgccg cgccaagcat ctcgctcaga
      721 ccctgcgcgg tcaggcgcgc atcgcgcgaa agcccggcgc cgttatcgat tacccagccc
      781 cgcgtatcaa tgttctgacc cttcagtact tccagtatgg cccgcgcgcc agactcagga
      841 gtagctcccg gccccgacgt ctccgcccct aaagttaatg acaaggtgcg cgccatcaca
      901 ttgttgctgt gcttgttaat tacgcgtatc aactcggcca ggttttcgga atcgtgccag
      961 acaatcggtt ttgcgctggc tggaaccaga cctgcacgca cgcctttgcc caaggttccc
     1021 cctgactcct tccataacaa tcgaaaaagg gccgagaaat actcgggttg agataaagcc
     1081 aggcgataaa cgctgaattc gccgcacgcg cccgccgccg tgccactgac atgtatcgtt
     1141 acgttattgc cctgagacga catagctgtc gcgaccgacg gcgcccccgg gcacaccgcg
     1201 gaactccatt taacatcgcc aatgacgcgg atgccaaaaa atggcggatc aataaccgga
     1261 atccatttct ttgcgcgggc gtcgggctga aacagcagac gcacggcgcc cagattcacc
     1321 atcattgcat cggggctggc gttataaggc cggtcaggcg ccccatcgaa ctggccgggg
     1381 tcggtggcaa cattgccgaa tatggatcga tcgacaatta cttcgctcaa gtttttaatg
     1441 ccgcgcagcc gtaattcgcg caacaatgac caaagacctt cgacagtaag aaacggatcc
     1501 ccgcccgctt tcaaatagag aggcccgcgc agcgatcctt gcgcgtcgat ctgggcgccg
     1561 ttattggcca gaaacgttgt gcgccagaca taatcgggtc ccagtccaga caaagcgctc
     1621 caggtagtga ccattttcat gaccgaggcg ggattgcgcg gcgtggatgg gttgatggct
     1681 accaggcgcg ggccatttat ttcctgcacg accagtgaaa gagcgctttc gggcaatgcg
     1741 gcggcgcgcc acgatttggc caggtcggtg ggcaaaccct gagccagcgc atttgcagca
     1801 aaaaggcagg caaggctcat cgccgccaac atccgcccac cgcgtttcat actgactcgc
     1861 tgttttctca aaaccatcca ggacataaca tccgcttccc aacgaaggcc acgaaagcat
     1921 gagcataacc aaagggagtt cattttgcgc tgaattggag gaaatttgaa acgacggctt
     1981 aaaatgcacg ctcattccat tcaaacggcg tcctgaacgc cgttaaccca caagacatta
     2041 cgcgtgaacc actcgcttca actttcccaa ttcgactatg tgctgccccc cgaacttatt
     2101 gcccagacgc ctacacccga gcgcacgggc agccggctat taaatctgga tgccgacggc
     2161 gcacttcatg attatcaatt cagcgacctg ccccggctgc taaaacccaa cgatttgctg
     2221 gtctttaacg acacccgggt catcaaggcc cgtctgcgcg gacaaaagga aagcggaggc
     2281 aagatcgaaa tactgatcga acgcattatt cgggaaaatg gcgcgctggc tcatatcaag
     2341 gccagcaaat cgcccaaggc cggcagcgct ctgatcattg acgaagattt ttcactaaac
     2401 gttctggggc gacaaggcga actgttcgaa ctggagtttc cgggccgagt tctggactat
     2461 ctggaacgtt acggcagcac cccgctgccg ccctatattg aacacgcggc gggacaagaa
     2521 gacgaagagc gctaccaaac cgtgtacgcc cgaaatccgg gagccgtcgc cgcccctact
     2581 gctggtttgc atttcgatca ggcgttgctt gcgcagttga caaaacaggg catccgtcaa
     2641 acgttcgtta ctctgcacgt cggagcgggt acttttcagc ccgtgcgcgt gcaagatctt
     2701 agccagcaca tcatgcactc tgaacactac acggtatcgg aggaaacgct tgctcaggtg
     2761 cgcgcaacgc acgattcggg cggaagagtc atcgcagtgg gcacgaccag cgttcgcgcc
     2821 ctcgaagcgg cagcaacccg ggcgagccag agtggcggca ttgaagacga cacccggctc
     2881 ttcatcacgc cgggctataa atttcatttc atagacgccc tgatcactaa ttttcacctt
     2941 cctcagtcaa ccttgctgat gctcgtttcg gcgctggccg gaatggagcc catccggcgc
     3001 gcctacgagc acgccgtcgc ggccagatat cgtttcttca gctacggcga tgccatgttc
     3061 atagagtctc cgaaataatg gctgggctgc aattcgatct tctggccacc gacggcctgg
     3121 cgcggcga
//
LOCUS       DATCXU010000330         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_830097,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000330 DATCXU010000000
VERSION     DATCXU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830097"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VIR04_07400"
     CDS             complement(<1..1159)
                     /locus_tag="VIR04_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460607.1"
                     /translation="MQSFTNAGAQWSSPRANVLKTTLLTAALLGAYGVAQGAGTEIFV
                     DGTNSSSSSPYNLDLATPPITKLSVTSGGFATGNGTVINGNLPNLASVTAGGSLTLSD
                     ALVTNTNSGPAMTANGAGAKITLSGANTVINWDGANVGGGGVSALRAVNGGVIDIDGG
                     TINTSGVAIADSLDRVRKQGILAADGGTVTAKNLTINTSGGFSPGVQAFRTYGNPTAG
                     DVPTVVSLDNVKINTTGSNYSVGIRADHAGASVVANGTDVNTTGAGSHGVEVDDGATV
                     ALTGGSITTSGVSASGVRAYTGSGAQGLLGPGVATVTGTKIITSGDNAAGVLAGDSAE
                     PTKGVVHLAGAGITTSGASTGGLTVAGGANGIEANYGGEIKSTGSMIAASGA"
     gene            complement(1694..2017)
                     /gene="iscA"
                     /locus_tag="VIR04_07405"
     CDS             complement(1694..2017)
                     /gene="iscA"
                     /locus_tag="VIR04_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY9460608.1"
                     /translation="MTITLTQRAAEHVSRHIEKRGKGLGLRLGVQTTGCSGMAYSFDY
                     VDEPRANDQVFECFGVKLFVDPESMPFLEGSELDYSRQGLNEGFKFSNPNEKGTCGCG
                     ESFTV"
     gene            complement(2014..2400)
                     /locus_tag="VIR04_07410"
     CDS             complement(2014..2400)
                     /locus_tag="VIR04_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY9460609.1"
                     /translation="MYSDKVLDYYEHPRNVGAFDESDGNVGTGTVGTLSGGQVIRLQI
                     KVGETGVIEDARFKTYGCGAAIAASSLATEWIKGRTLDQAISIHSTQIAEELALPPIK
                     IHCSISAEDAIKAAVRNYREKQQEQS"
     gene            complement(2434..3645)
                     /locus_tag="VIR04_07415"
     CDS             complement(2434..3645)
                     /locus_tag="VIR04_07415"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY9460610.1"
                     /translation="MSTRAVYLDNSATTPVDPRVVEKMVPWLYERFGNPASNSHTYGW
                     DAEDAVEQARAQVARLVNADTREIVWTSGATESDNLAIKGAAEAYSGRGKHIVTVKTE
                     HKAVLDTCHHLERQGFELTYLDVGANGLVDIDAFRNALRPDTILASVMLVNNETGVIQ
                     DVGSLGKICRENGVVFHVDAAQATGKVAIDLQALDVDLMSFSAHKTYGPKGVGALYIR
                     RKPRIRLEAQMHGGGHERGFRSGTLATHQIVGMGYAYELARVEMLTENERIRMLRDRL
                     WAGLSKIEAVYLNGDLNHRVVHNLNVSFAYVEGEALMMSLKGLAVSSGSACTSASLEP
                     SYVLRALGRSDELAHSSLRFTLGRFTTSDEIDFAIGLLCTQVARLRDISPFWEMTRAG
                     IDLASVQWATS"
     gene            complement(3681..>3922)
                     /locus_tag="VIR04_07420"
     CDS             complement(3681..>3922)
                     /locus_tag="VIR04_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY9460611.1"
                     /translation="SCGGKEDCAIGRNGASGKCMTHELWSALSRKMVDYLDSVSLQDQ
                     VDQQRMRLLQEASLAQAAARLNRSPNVKIVTSAAA"
BASE COUNT          835 a         1190 c         1005 g          892 t
ORIGIN      
        1 cggcgccgga cgccgcaatc atggagcccg tgcttttaat ctcgccgccg taattcgctt
       61 ctattccgtt tgcgccgccc gccacagtaa gcccgcctgt gctggccccg gaagtagtga
      121 tacctgctcc ggcaagatgg acgacacctt ttgtcggctc ggctgaatcg ccggccaata
      181 ccccagcggc attgtcgccg ctggttatta tcttcgtacc cgtaaccgtg gcaacccccg
      241 gtcccagcaa tccctgggcg ccgctacccg tgtaggcacg cactccactg gccgagacgc
      301 ccgaagtggt gatactgcca ccggtcagtg ccacggtggc gccatcatcg acctcgacac
      361 catggctgcc cgcccccgtc gtgttgacgt ccgtgccgtt ggcaacaacg ctggcgcccg
      421 cgtggtcagc tcgtatgccg accgaataat ttgaccccgt ggtattgatt ttgacattgt
      481 ccaggctgac aactgtagga acatcgcccg cggtggggtt gccgtacgtt ctaaatgcct
      541 gcacacctgg gctaaagccg ccgctggtgt tgattgtgag gttcttcgcc gttacagtgc
      601 ccccgtctgc cgcaagtatt ccctgcttgc gtacacggtc cagagagtct gcaatcgcga
      661 cgccggatgt gttgatcgtg ccgccatcta tgtctatgac tccaccattg acggcccgga
      721 gtgcggacac gcccccaccg cccacattgg cgccatccca gtttattacg gtattcgcgc
      781 cggacagcgt gatcttcgcg ccagctccat tggctgtcat ggccgggccg ctattggtgt
      841 ttgtcacgag cgcgtcgctc agcgtaaggc tgccgcccgc ggtgacactg gccagattgg
      901 gcaagttgcc attaataacc gtcccattac ccgtcgcaaa tccgcccgag gtcacgctca
      961 gtttcgttat cggcggtgtt gcaaggtcga ggttataggg agaacttgaa ctggagttgg
     1021 ttccatccac gaaaatctcg gtccccgcgc cctgcgcgac cccatacgcg cccagcagtg
     1081 cagccgtcag taaggttgtt tttaaaacat tcgcgcgcgg tgaactccac tgcgcaccag
     1141 cattagtaaa gctttgcact tgatttcccc ttggccaatg atttaggccg ttacaagcga
     1201 tacgccataa aagcttgtcg tcacacccca catgcaacga ttcgccgcta aaaacgggtt
     1261 ccaaacgagc tacgacagca acacactgcg gcgaaataac tgctaaagcg gccacgttgc
     1321 cgcgttactt attcattgca ttcttgtaaa caaatgaacc aattatccaa aaccaggaaa
     1381 tagatcgaac ataatgtaac ctgaacagaa cctgaatact acccgaagta tcatgttttg
     1441 tcgaaaccgt tcagccggat aacggcaaaa attgccctca attcgttttc tgtgattttt
     1501 tgagcgcttc ccgcatgcgt ccgcgctgtg ctcgtccgta cagacgtcaa gatttaactt
     1561 gcaaaggcaa tgagccaccg gcctggatgg cctgtagcga tctaattttt tattattgat
     1621 cttgggcgat taacatgtcg ccgtggccgc cgttcgccgt ttttcacatg cgtgcggcca
     1681 agtttggcaa attctagaca gtgaatgatt caccgcaccc gcaggtgcct ttctcgttcg
     1741 gattcgaaaa cttgaacccc tcgttcagac cctgacgcga ataatcgagt tcggagcctt
     1801 caagaaaagg catgctttca gggtcgacaa ataattttac gccgaagcat tcaaacacct
     1861 gatcgttcgc cctgggttcg tcgacatagt cgaaactgta agccatgccc gaacaacccg
     1921 tggtctgaac gcccaagcgc aagcccaagc ctttgccgcg cttttcgatg tggcggctga
     1981 cgtgctcggc cgcccgctgt gtaagggtaa tagtcatgat tgttcctgtt gcttttccct
     2041 gtaatttcgc accgcggcct tgatcgcgtc ttcggccgaa atcgaacagt gaatttttat
     2101 cggtggcaaa gccaattctt cggcaatctg cgtactgtgt atgcttatag cctgatccag
     2161 ggttctgccc ttgatccatt cagtcgccaa agagcttgcc gcaatggccg cgccacaacc
     2221 atacgtttta aatcgggcat cttcaataac acctgtttcg cccaccttga tctgcaaccg
     2281 tatgacctga ccgccggaga gtgtgccgac cgtcccggtg cccacattcc catcggattc
     2341 atcgaacgcc cccacattgc gaggatgctc gtagtaatcc agtaccttgt cactatacat
     2401 gaaccccatg gcgatagatt cccggattcc ccgctagctc gttgcccatt gaactgaagc
     2461 caggtcaatt cccgcccgtg tcatttccca aaagggcgat atatcgcgca acctggcgac
     2521 ctgggtgcag agcagcccaa tggcaaaatc gatttcatcg gaagttgtga agcgcccgag
     2581 cgtgaaccgc aaggaactgt gcgccagttc gtcgctgcgg ccaagcgccc gcaatacgta
     2641 tgacggctcg aggctggccg aggtgcaggc cgacccgctg gaaactgcca ggccttttag
     2701 ggacatcatg agcgcttcgc cttcgacata ggcaaaactg acgttcagat tatgcaccac
     2761 gcggtggtta aggtcgccat tcaaataaac agcctcgatc ttcgacaacc cggcccacag
     2821 gcgatcgcgc agcatgcgga ttcgttcgtt ttccgtcaac atttccaccc gggccagctc
     2881 ataggcgtag cccatgccaa caatctggtg cgtggccaac gtgcccgaac gaaaaccccg
     2941 ctcgtggccg ccgccgtgca tttgcgcttc gaggcggatt cgcggcttgc ggcgaatgta
     3001 caacgcgccc acccctttcg gtccatacgt tttatgagcc gaaaacgaca ttaaatcaac
     3061 atcgagcgcc tgcagatcaa tagcaacttt accggtcgcc tgagcggcat cgacatgaaa
     3121 tacgaccccg ttttcgcgac aaatcttgcc cagcgaccca acatcctgga tgacgccggt
     3181 ttcgttatta acgagcatga cagacgccag aatcgtgtcc gggcgcagcg cgttcctgaa
     3241 tgcgtcgatg tcgaccaagc cgttcgcgcc tacatccaga tacgtcagtt caaacccctg
     3301 acgctcgaga tggtgacagg tgtccagaac ggccttatgt tcagttttta ccgtgacaat
     3361 gtgctttccg cggccggaat acgcttccgc agcgcctttg atcgcgaggt tgtccgattc
     3421 ggtcgcgccc gaagtccaga ctatttcgcg cgtgtcggcg ttgaccagcc gcgcgacctg
     3481 cgcgcgcgcc tgttcgacag catcttcggc atcccacccg tatgtgtggc tgttcgatgc
     3541 gggattgccg aaccgttcat atagccaggg aaccattttt tcgaccaccc gcgggtccac
     3601 gggagtggtg gcggaattat cgagataaac agcgcgcgtt gacacgaagc gactcctaac
     3661 ctttaggcag ccgctaaaat tcaggcagcc gccgaagtga caatcttaac atttggggat
     3721 cggttaagtc gagccgccgc ctgggccagc gaagcttcct gaagaaggcg catgcgctgc
     3781 tgatcaacct gatcctgcag ggagacggaa tcaaggtaat cgaccatttt tcgacttaac
     3841 gccgaccata attcgtgcgt catgcacttg cctgaggcgc catttcgacc gatggcgcaa
     3901 tcttctttgc cgccgcagct tg
//
LOCUS       DATCXU010000331         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_769106,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000331 DATCXU010000000
VERSION     DATCXU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769106"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VIR04_07425"
     CDS             complement(<1..165)
                     /locus_tag="VIR04_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460612.1"
                     /translation="MTKDVNAYALRAVDLVRTYEESGSRVEVLRGVSLHVDQGEMVAI
                     VGASGSGKSTL"
     gene            complement(158..1429)
                     /locus_tag="VIR04_07430"
     CDS             complement(158..1429)
                     /locus_tag="VIR04_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY9460613.1"
                     /translation="MSYELWIGARYAGLARARRQGGGHDRFVSFIAASSMAGIALGVA
                     ALIVVLSVMNGFQTQVRDRMLSVLPHIELYVPGVAPEEVLGHWRQIAHDAEKNKEVKG
                     AAPFVAAQGMVVRGESLSGVQIRGIDPPLEASVSDLGNQMIDGDIKSLTPGSFSVVLG
                     NQIAASLGVHTGDTVLVLAPQGSVSPAGFAPRMRQFTVSGIFSSGYYEYDASLAFVNY
                     EDASKVFRDSGASGVRLRIADMQKAPLIAQELMKILPPFVQAKDWTQNNRTWFAAVQT
                     EKRMMFLILALIVAVAAFNLLSSLVMAVKDKQSDIAILRTLGANPNEIARIFLVQGSL
                     IGVVGTLLGVGFGMALAYNIDVIVPFIERLLGVQFLPQQIYFISELPSNPQLSDIVTI
                     AVTSLILSLLATLYPSWRASRLQPAQVLRHD"
     gene            1472..2443
                     /locus_tag="VIR04_07435"
     CDS             1472..2443
                     /locus_tag="VIR04_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460614.1"
                     /translation="MQIVLPGALPDPREARELIPYLLKAAPTLANWLQYSHAAIKPAN
                     PAETGCTPYEQWLLGALGFEPGAGQNLSSGLGPLWLNSSAPSGHIPGPPAKSDQIVWL
                     TELVHVSPSRDGAALLPARELAISADQSVALFESAQRLFEGTGFALHHTGVERWRIDI
                     PKDFAPRCASPLLVSITSVNDWWPQDIAARPWRRLVNELQMLWFENPVNQTRAAQGLA
                     PVNSLWLFGGAAANQFPDKRTRKETKIYDALLAPSLKHDWGAWVDALTELERRVFQPM
                     AQHKNMPGIVLIGRERIIELKPKALGRWTRWLPGGTLTWSKWWSPQN"
     gene            2425..4134
                     /gene="recJ"
                     /locus_tag="VIR04_07440"
     CDS             2425..4134
                     /gene="recJ"
                     /locus_tag="VIR04_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY9460615.1"
                     /translation="MVTPKLSDRCVNYPAAQALEAAGIHPLLSRLWAARGVTRASETH
                     PEWSALIPPAQLTQSDHAAKLLADAIQAKKRLLIIADYDCDGATACAVGLRALRSMGA
                     NVDFLVPNRFETGYGLSPAVVNLALQHRNGKPDILITVDNGIASVDGVQAANLAGIGV
                     IITDHHLPGDRLPEALVIVNPNQPGCGFRSKNLAGVGVIFYLMLALRAEFRRRGAYPG
                     DGGPRLDALADLVALGTVADVVKLDANNRLLVTQGLQRMRTGRMHAGVRALFAVAARD
                     PSQASGFDLGFALGPRINAAGRLADMSLGINCLITDDENLALQLARELDAMNQERRSI
                     ETAMRAQALAAFEESTPTVGATVCVYNPDWHQGVIGLVASRLKETYWRPALAFARSDS
                     GELRGSGRSIPDVHLRDVLDLVSKRHPGIILKFGGHAMAAGLTLREDAYNLFTSSFED
                     AVKELSGKALFGPTIETDGSLETGYANAEVAGLLQRQVWGAGFPAPIFRDTFQVRQQR
                     LLKEKHLKLNLERGHQRFDAIWFNHADALPEYVEVAYRLDQNVWNGLVTAQLIIEYAQ
                     AAD"
     gene            4163..5032
                     /locus_tag="VIR04_07445"
     CDS             4163..5032
                     /locus_tag="VIR04_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY9460616.1"
                     /translation="MKFGNLNVGGKAQVCVVDTEKNEYWPLADLVPGFNSDMTELVRD
                     YKQIKPKLQTQGAGKTLDASQVLAPIPRPRRNIFCVGRNYHAHAKELSSSVFKDNDKD
                     TKAWPIVFTKVPECVVGPNDHVKLPGAISQQIDYEAELAVIIGTGGKNITKDKAMSHI
                     FGYTIVNDVTARDVQIRHQQWDMGKSFDTFCPMGPWIVTADELDGNNTRVRCWVNGDL
                     RQDGLTPDLIFDLSTLVETCSRGITLYPGDMIATGTPAGVGMGMKPPQYLKTNDVVRI
                     QIDGIGILENTFK"
     gene            5064..>5688
                     /locus_tag="VIR04_07450"
     CDS             5064..>5688
                     /locus_tag="VIR04_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460617.1"
                     /translation="MKSKISRTPWLKAVCAIAAIGFSGYACAQYPDRAITMIVPFPPG
                     GVADTVARPLASAMSQELGQPVVIENKAGAGGAIGIGTAARAKPDGYTVLLSLSSISI
                     LPEADKILNRKAAFQLDQFKTVARITADPTVLVVRADAPWKTTKEFIESAKKHPGDIT
                     YGSSGIYGTMHVPMEMLAHAADVKLTHVPYTGAGPAVGALLGNQVQAL"
BASE COUNT         1304 a         1729 c         1598 g         1057 t
ORIGIN      
        1 caaagtgctt tttcccgatc ccgaagcgcc cacgatagcc accatttcgc cctggtcgac
       61 atgcaggctg acgccgcgca ggacttcgac tcgcgagcca ctttcttcat aagtcctgac
      121 cagatcgacg gcgcgcagcg cgtacgcatt tacatcctta gtcatggcgc agcacctgcg
      181 cgggctgcaa gcgcgaggcg cgccagcttg ggtataacgt cgcaagcaga gaaaggatca
      241 aggaggttac ggcgatcgtg acaatgtcgg acaactgcgg attggaaggc agctcgctga
      301 taaagtagat ttgttgcggc aagaattgca cccccagcaa acgttcaatg aacggcacga
      361 tcacatcaat attataggcg agcgccatgc caaaacccac gcccagcaag gtgcccacca
      421 cgccgatcaa tgagccctgc actaaaaata ttctggcgat ttcgttggga tttgccccca
      481 aagtacgcaa aatggcaatg tcggactgtt tatctttcac cgccatcacc agtgacgaca
      541 acaagttgaa cgccgccacg gccacaatta aagccagaat taaaaacatc atgcgcttct
      601 cggtctgcac ggcggcgaac caggtgcggt tgttctgcgt ccagtcttta gcctgcacga
      661 agggcggcag gatcttcata agctcttgcg caatcagcgg cgctttttgc atgtcggcaa
      721 tgcgcagacg cacgccactg gcgccgctgt cgcgaaatac cttggaagca tcttcgtagt
      781 tcacaaacgc cagtgacgca tcgtattcgt aatagcctga agaaaagatt cccgatacgg
      841 tgaattgccg catgcgcggc gcaaagccag cgggcgaaac cgaaccctgc ggcgcaagca
      901 ccaataccgt atcgccggta tgcacaccca gggacgcggc aatctggttg cccaggacaa
      961 cggaaaagct gcccggtgtc agggatttaa tgtcgccatc gatcatctgg ttgccaaggt
     1021 cggacacaga cgcctccagg ggtggatcga tgccgcgaat ctgaacaccg cttaacgatt
     1081 cgccacgcac aaccatgcct tgcgcagcca caaacggagc ggcccctttg acttctttat
     1141 ttttttccgc atcgtgcgca atttgccgcc aatgacccaa tacctcctcg ggcgcaaccc
     1201 cgggcacata caactcaata tgcggcaaaa cggacaacat gcgatcccgc acttgcgtct
     1261 ggaagccgtt catgacggaa agcaccacga tcagggccgc cacacccaac gcgatgccag
     1321 ccatggaact ggccgcgatg aacgacacaa aacgatcatg cccgccgccc tgccgtctgg
     1381 cccgggccag tccggcgtag cgcgctccta tccacaattc atacgacatg gtcattaatc
     1441 accgtttcga cataaacatt acaattccgg aatgcaaatc gtactccctg gcgcgctacc
     1501 ggatccccgc gaagcgcgcg aactgattcc ttacttgcta aaggccgctc cgaccctggc
     1561 aaactggctg caatacagtc atgccgccat taaaccagcc aatcccgccg aaacaggctg
     1621 cacgccgtac gaacaatggc tacttggcgc gctcggattc gaacccggcg cggggcaaaa
     1681 ccttagctct ggcctgggcc ctttgtggct caacagctcc gccccatccg gccacatacc
     1741 cgggccgccc gcgaaatccg accagatcgt ctggctgacc gagctggtcc atgtatctcc
     1801 atcgcgtgat ggcgccgctt tgctgccagc acgggaattg gcgatttcag cggatcaaag
     1861 tgtagcgtta ttcgaatcgg cgcaacgctt gttcgaagga acaggctttg ccctgcatca
     1921 taccggtgtc gagcgctggc gtatcgacat ccccaaagat ttcgcacccc ggtgcgccag
     1981 cccgctgctg gtgagcataa cctcggtcaa cgactggtgg ccgcaggata tcgccgcgcg
     2041 gccatggcga cggctggtca acgaactgca aatgctgtgg tttgaaaacc cggtcaatca
     2101 gacccgcgcg gctcaaggac tggccccggt caatagtctt tggctatttg gaggcgccgc
     2161 cgctaaccag ttcccggaca agcgcacacg caaagaaacg aaaatttacg acgcgttgct
     2221 ggcccccagc ctgaaacacg actggggcgc ttgggtcgac gcgctcaccg aacttgaaag
     2281 acgcgttttc cagcctatgg cgcaacataa aaacatgccc ggaatcgtgt tgatcggccg
     2341 tgagcgcatt attgaattaa agccaaaagc gctgggccga tggacccgct ggttgccggg
     2401 cggcaccctg acatggagca agtggtggtc accccaaaac taagcgatcg ctgcgtcaat
     2461 tacccggcgg cgcaagccct ggaagcggcg ggcatccacc cgctgctttc gcgcctgtgg
     2521 gcggcgcgcg gcgtaacgcg cgccagcgaa acacaccccg agtggtctgc cctgatcccg
     2581 cccgctcagc tcacccaaag cgaccacgcc gcaaaactgc tggccgacgc gattcaagcc
     2641 aaaaagcgct tgctaatcat tgccgactac gactgcgacg gcgcaacggc ctgcgctgtg
     2701 ggtctgcgcg ctttgcgcag catgggcgcg aatgtcgatt ttctggttcc taatcgcttc
     2761 gaaaccggct acggcctgtc gcccgccgtg gtcaatctgg cgctacagca ccgaaacgga
     2821 aagcccgata tcctgatcac cgtcgataat ggcatcgcga gcgtcgatgg cgttcaggcc
     2881 gcgaacctgg cgggcatcgg ggtgatcatc accgaccacc atcttcccgg cgacaggctg
     2941 cccgaagcgc tcgtgatcgt caatcccaac cagccaggct gcggctttcg ctccaaaaat
     3001 ctggcgggcg tgggcgtcat cttttatctt atgctggcgc tgcgcgccga attccggcgg
     3061 cgcggtgcgt accccggcga cggcggcccc cggctggacg ccctggcaga cctggtcgcg
     3121 ctgggaacgg tggccgacgt ggtaaagctt gacgccaata atcggctgct cgtaacgcag
     3181 gggctacaac gaatgcgcac cggtcgcatg cacgcgggcg tgcgcgcgct attcgcggtg
     3241 gcggcgcgcg atcccagcca ggcgagcggg ttcgatctcg gttttgcgct cggcccaaga
     3301 attaacgcgg cgggacgcct ggccgatatg agtctgggca ttaactgcct gatcactgac
     3361 gacgaaaatc tcgcgctgca actggcccgg gaacttgacg ccatgaacca ggagcgccgc
     3421 tcgatcgaaa cggccatgcg cgcgcaagcg ctggcggcgt tcgaagaatc cacccccacc
     3481 gtgggcgcaa cagtgtgcgt ttacaacccg gactggcatc agggtgtaat cgggctggtg
     3541 gcttcaaggc taaaggaaac ctactggcgt ccggcactgg cgttcgcccg ctccgactcg
     3601 ggcgagctgc gcgggtcggg ccggtcgatc cccgatgtgc atctgcgcga tgtgctcgat
     3661 ctggtctcca agcgccaccc cggcatcatc cttaaatttg gcggtcacgc catggcggcg
     3721 ggcctgacgt tacgcgagga cgcttacaac ctgttcacaa gcagctttga agacgcggtc
     3781 aaagaactaa gcggcaaggc gctatttggc ccgaccatcg aaaccgatgg ctcgcttgaa
     3841 accggttacg ccaacgccga ggtggcaggg ctgttgcagc ggcaggtatg gggggcgggc
     3901 tttccggctc cgattttccg cgacaccttc caggtgcgcc aacaacgcct gttgaaagaa
     3961 aaacatctga aactgaatct ggagcgcggc catcaacgct ttgacgccat ctggttcaac
     4021 catgccgatg ccctgcccga gtatgtggag gttgcgtacc ggctggacca aaacgtgtgg
     4081 aacggactgg tgacggcgca attgattatt gaatacgcac aggcagccga ttagaatcag
     4141 tatttttacc caagggacac ccatgaaatt cggtaattta aatgtcggcg ggaaagcgca
     4201 agtttgtgta gtcgatacag agaaaaatga atactggccc cttgcggacc tggtccctgg
     4261 atttaattcc gacatgaccg aactggtgcg tgattacaaa cagatcaaac cgaaacttca
     4321 aacacaaggc gcgggaaaaa cgctggacgc atcgcaagtg ctcgctccta tcccccgccc
     4381 acgccgcaat attttctgcg taggccgcaa ctatcacgcg cacgccaaag agctgtcgag
     4441 ctcggtattc aaagacaacg ataaagacac caaggcatgg ccgatcgttt tcaccaaagt
     4501 tcccgaatgc gtggtggggc caaacgatca tgtgaaattg ccgggcgcga tctcgcaaca
     4561 aatcgactac gaagcggagc tggcggtcat catcggaacc ggcggaaaaa acatcacgaa
     4621 agacaaggcc atgagccata tctttggcta cacaattgtc aacgatgtca ccgcgcgcga
     4681 cgttcaaata cgccatcaac aatgggatat gggcaagtca ttcgacactt tctgccccat
     4741 gggtccatgg atcgtaacgg ccgacgagct ggacggcaac aatacgcgcg tgcgctgctg
     4801 ggtgaatggc gacttgcgcc aggatgggct gacgcctgat ttgattttcg atctgtcgac
     4861 cctggtggaa acctgttcgc gcggcattac gctatatcca ggcgacatga tcgccaccgg
     4921 cacgccggcc ggagtgggaa tgggaatgaa gccaccccag taccttaaaa caaacgatgt
     4981 cgtccggatt cagatcgacg gaatcggcat tctggaaaac acattcaaat agataaacgt
     5041 tcgatcgttc aagaggaggc aatatgaaat caaaaataag ccgcacgccc tggctaaagg
     5101 ccgtttgcgc catcgcggcc attggttttt caggctatgc ctgtgcgcaa taccccgatc
     5161 gcgccatcac catgatcgtg cccttcccgc ccggcggcgt cgccgacacg gtcgcgcggc
     5221 ctttggccag cgcgatgtcg caagaactcg ggcagccggt cgttattgaa aacaaagccg
     5281 gggcgggcgg cgcgataggg ataggcaccg cggcacgagc gaaacccgat ggctatacgg
     5341 tgctgctaag cctgtcgtcc atttcgattt tgcctgaagc cgacaaaatc ctgaaccgca
     5401 aagccgcctt ccaactggat caattcaaaa cggtggcccg catcaccgcc gatccgacgg
     5461 tgctggtagt gcgtgctgat gctccgtgga agacgaccaa agagttcata gaatcggcga
     5521 aaaagcaccc tggagacatc acctacggca gttcgggcat atacggaacc atgcatgttc
     5581 cgatggaaat gctggcgcat gccgccgacg tcaaattgac ccacgtcccg tacacaggcg
     5641 cggggcctgc ggtgggcgcg cttttgggca accaggtgca agccctgg
//
LOCUS       DATCXU010000332         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_329143,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000332 DATCXU010000000
VERSION     DATCXU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329143"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1063
                     /locus_tag="VIR04_07455"
     CDS             449..1063
                     /locus_tag="VIR04_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY9460618.1"
                     /translation="MLIVSASPFIRLGLRSILKEMGVASVLHETPTAREAQSCITPDL
                     DLVIMDPELPDMNPISFVQQVRRQAELAPILFCGGRSATLYASLAMKLNVDGYLGDLA
                     DEKTIGATIRTVLGGMRCFPRREAADALSQKMQALSRKELTVLLLLRQGLRNKDIAQR
                     LFLSEKTISAHKQKVLHKLGVNAITQISETRAPADFVNETPQLF"
     gene            1488..1670
                     /locus_tag="VIR04_07460"
     CDS             1488..1670
                     /locus_tag="VIR04_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY9460619.1"
                     /translation="MRKFIQSLARDERGVSALEYAILAGLIVGVLVTAVGVFSGDLSA
                     AFSNLGAAVTTATTPK"
     gene            1697..2209
                     /locus_tag="VIR04_07465"
     CDS             1697..2209
                     /locus_tag="VIR04_07465"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY9460620.1"
                     /translation="MINLPSSVAFLVLPVLFSAGWTLATLAYRDLSLRRLPNGWVAAY
                     AGLFPLYAWASGMGWAQVGWHAAFGMAAFAFTILLFVLRILGGGDVKLWGAIMLWAGP
                     QGAASALVMATLCGSLLGVLGWLSRGILRRKRRPAGAAILRMLTAARGVPYGAGLAIA
                     GIHTLVMAGL"
     gene            2248..3171
                     /gene="cpaB"
                     /locus_tag="VIR04_07470"
     CDS             2248..3171
                     /gene="cpaB"
                     /locus_tag="VIR04_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY9460621.1"
                     /translation="MSTTLKFVLLVIGAALLALTARTLFANMTNRQKPVEISVQVRVA
                     ASDLPAGLLLRNEDLAWKPLAQSNVPEGALVKGTPGAQATTGVLVRHDTKAGAFVLER
                     DIIRPDAPGFLAAVLKPGMRAVSVPVDNVSGNAGLIQPGDYVDMILVQQSRKNEDLNS
                     ERARSVVSETVVEKVRVIAVGSVFEHQPKVADKHNSSQIARTVTIEVTPRAAEAVTVA
                     SRLGTLSMALRSFALADRNAPHTESVESVVAWDGEKKSAPVWAGDVSRAIGAARAPEV
                     VVKAPVPKAAPHEITIYRGSKKDQLTLGGAR"
BASE COUNT          754 a          913 c          916 g          752 t
ORIGIN      
        1 gcctgccgtg aatcttaccc ttgccataat ccggattcct gtgtaacccc tgtgtaaccc
       61 gaatcatact atatcaatga atatcaatca accttcttat ttgtgtaatc cactggaaag
      121 ccgcatattt actaggtttt gtagctttta atcaacatgg ataaataccc aaaaaaccca
      181 tatccttctt attcgtaatg agaaggtcga gggttcgact cctttcatcg gcaccaatcg
      241 attagataga catacgccaa tctgcatggg ttggcgtttt tgctttctga ccatcactaa
      301 agtggcagct atcctccata tgcgacaaaa ttcttcgagt ttgtacacaa aagtgaaaaa
      361 atgatgtaat ttcacatatt ggttacgtcc gtttggcgcc atcattcgat gtaaaccgtt
      421 acgcttaagg tgtacaggta tgggatcaat gctaattgtg agtgcatcgc ccttcattcg
      481 gcttgggctg cgctccatac tcaaagagat gggggtagcc tcagtcttgc acgaaacgcc
      541 cactgcgcgc gaagcccaaa gctgcatcac ccccgatctc gacctggtca tcatggatcc
      601 ggaactgccg gatatgaatc caatctcgtt tgtacaacaa gtgcgtcgcc aggccgaact
      661 ggcgcccatt ctgttttgcg gaggccgaag cgcgactctg tatgcctctt tggcgatgaa
      721 actgaatgtg gatggatatc tgggcgatct tgcggatgaa aaaaccatag gcgcaacgat
      781 ccgcactgtt ctgggcggga tgcgatgctt tccccgccgg gaagcggccg atgcgctttc
      841 ccaaaagatg caggcgcttt cgcgcaaaga actgactgtg ctgttgctgc tacgtcaagg
      901 tctgcgcaat aaagatattg cgcagcgcct gtttctaagc gagaagacca tcagcgcaca
      961 caagcagaaa gtcttgcaca aattgggcgt caacgctatt acacagatta gcgaaaccag
     1021 agcccccgca gatttcgtca acgaaacccc gcaactgttc tgattagtta gctgttgcct
     1081 tccgcagagc acggaccggc acaacaaaag ccggatctga tccgactaac cgccgcacgc
     1141 cggcaaatcc caaaaacata aagcactaaa tcggtgcctt cgcatgcccg caaaccccgc
     1201 gcgggcgcat accccaattt gcgcatgatt aggtcaattg accggatgct tagggtaatc
     1261 ggatggactt tggattggcc ataaggttta gtcgttacgc gccgattctt aatccgcaca
     1321 cgtaaaaagt aagaattttc tgtttttgat tgcaacacca tttacatctt gtcttgctat
     1381 gtagaattaa ttcacccgat gcagcaaaag ggcaaaacca ccgggacctt ttggccacgg
     1441 taattttttt cacgacatag tgtcgtgcaa attttcggag agatcctatg cgtaaattca
     1501 ttcaatccct ggcccgcgat gagcgcggtg tcagcgccct tgagtatgcc attttagcgg
     1561 gccttattgt gggggtcctc gtaacggcgg taggcgtgtt cagtggcgac ctttctgccg
     1621 ccttctcgaa ccttggcgct gcggttacga ctgcaacgac tcccaagtaa ttcgcgaccc
     1681 acaccaagca cacatcatga tcaacctgcc ttcgtcagtt gctttcctgg tcctcccggt
     1741 cttgttcagc gccggatgga cattggcgac attagcctat cgggaccttt cccttcgccg
     1801 cctgcccaac ggatgggtcg ccgcttacgc gggcttgttt ccgttgtatg cctgggcaag
     1861 cggaatggga tgggcgcagg tggggtggca tgccgctttc gggatggccg cattcgcatt
     1921 cacgattctg cttttcgtgt tgagaatact gggcggaggc gatgtcaagt tgtggggcgc
     1981 gatcatgctg tgggctgggc ctcagggagc cgcttcggcg ttggtgatgg caacgctgtg
     2041 tggctccctg ctgggcgttc tgggctggtt gtcgcggggc atcctgcggc gaaaacgcag
     2101 acccgccgga gcggcgattt taaggatgct gactgcggcg cggggtgttc catacggagc
     2161 gggcctggca atagcgggaa ttcacacact ggtgatggca ggactatagg cggcacgaga
     2221 ttgcgctgaa cccaagggag ataagaaatg tcgacaacac tgaaattcgt cttgctggtc
     2281 attggggcgg cattgctggc gctgacagcc cggacgctgt tcgcgaatat gacgaaccgc
     2341 caaaagccag ttgaaatatc cgtgcaggtg cgtgtcgccg ccagcgactt gccagccggc
     2401 ctgctattgc gcaacgaaga tctggcctgg aagccgctgg cccagtcaaa cgttcccgaa
     2461 ggcgccttgg taaaagggac gccgggagca caagccacaa ccggcgtttt ggtgcgtcac
     2521 gacaccaaag ccggagcatt cgttctggag cgcgacatta tccggcccga tgcccctggc
     2581 ttcctggccg ccgtgttgaa acccggcatg cgagccgtgt cggtgccggt ggataacgtg
     2641 tccggcaatg cgggactgat ccagcccggc gattacgtcg acatgatcct ggtgcagcaa
     2701 tccaggaaga acgaggatct gaatagtgaa cgcgcacgtt ctgtcgtaag cgaaaccgtg
     2761 gtggaaaaag tccgggtaat tgccgttggc tcggtatttg agcaccagcc caaagtcgcc
     2821 gataaacaca atagcagtca gatcgcgcgc actgtaacga tcgaggtcac gccacgtgcg
     2881 gcggaagccg taacggtcgc gtcccgtctg ggcaccttat cgatggcgtt gcgcagtttc
     2941 gcactggccg atcgcaatgc accgcacact gaatctgtcg aatccgtcgt cgcgtgggat
     3001 ggcgagaaaa aatcagcgcc agtttgggcg ggcgacgtgt cgcgcgctat cggcgccgcc
     3061 cgcgcccccg aggtcgtcgt caaggcgccc gtccccaaag cggcgccgca cgagatcacc
     3121 atttatcgcg gatccaaaaa ggaccaactt accttgggcg gggctcgctg agtctgtctc
     3181 actattacag aaaaaaggca atgaccaatg attgcgaata taccaagcag gaagtcgtat
     3241 ctccggacga ctctgtacag cgcggctctg acatttggcg ccctgaccat gggccacgcc
     3301 gcgccgacga cggctccagt cccgatgccg gcaaa
//
LOCUS       DATCXU010000333        11134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_88411,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000333 DATCXU010000000
VERSION     DATCXU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88411"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="rpoA"
                     /locus_tag="VIR04_07475"
     CDS             complement(<1..986)
                     /gene="rpoA"
                     /locus_tag="VIR04_07475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY9460622.1"
                     /translation="MASQGFLKPRSIEVVPVGKNHARVVMEPFERGYGHTLGNALRRI
                     LLSSMAGYAPTEVQITGVVHEYSTITGVREDVVDILLNLKGVVFKLHNREEVSLTLRK
                     QGQGVAVAGDIELPHDVEIINPEHVIATLTDAGKLEMQIKVEQGRGYVPGNVHALVDD
                     RSHTIGRIVLDASYSPVRRVSYAVDNARVEQRTDLDKLILDIETNGVLSPEEAVRQSA
                     RILMDQISVFAALEGAGPDVDFQRDGGKLPQIDPVLLRPVDDLELTVRSANCLKAENI
                     YYIGDLIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLENWPPLGLER"
     gene            complement(1139..1762)
                     /gene="rpsD"
                     /locus_tag="VIR04_07480"
     CDS             complement(1139..1762)
                     /gene="rpsD"
                     /locus_tag="VIR04_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY9460623.1"
                     /translation="MARYIGPKCKLSRREGTDLFLKSSRRSLDSKCKLESKPGQHGRT
                     SGARTSDFGVQLREKQKLKRMYGVLEKQFHKYFVEAERRRGNTGETLIQLLESRLDNV
                     VYRMGFGATRAEARQLVNHRTVEINGRTADIASMSVKAGDVVSIREKSKGQLRIRESL
                     DLATGQGLPQWVDVDATKMAGTFKQIPDRSEVARDVNESLVVELYSR"
     gene            complement(1774..2175)
                     /gene="rpsK"
                     /locus_tag="VIR04_07485"
     CDS             complement(1774..2175)
                     /gene="rpsK"
                     /locus_tag="VIR04_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY9460624.1"
                     /translation="MAKAPTSGATRVRKKVKKSVSDGIAHVHASFNNTIITITDRQGN
                     TLSWATSGGAGFKGSRKSTPFAAQVAAESAGRLALEYGIKTLEVRIKGPGPGRESSVR
                     ALNALGIKISSISDITPVPHNGCRPPKRRRI"
     gene            complement(2189..2554)
                     /gene="rpsM"
                     /locus_tag="VIR04_07490"
     CDS             complement(2189..2554)
                     /gene="rpsM"
                     /locus_tag="VIR04_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY9460625.1"
                     /translation="MARIAGINIPPHQHAEIGLTAIFGIGRTRARKICEASGVDFSKK
                     VKDLTDSELERIRECINVLTVEGDLRREVQLSIKRLVDLGTYRGMRHKRGLPVRGQRT
                     RTNARTRKGPRRAAASLKK"
     gene            complement(2590..2703)
                     /gene="rpmJ"
                     /locus_tag="VIR04_07495"
     CDS             complement(2590..2703)
                     /gene="rpmJ"
                     /locus_tag="VIR04_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY9460626.1"
                     /translation="MKVMASVKRICRNCKIIKRHGVVRVICTDPRHKQRQG"
     gene            complement(2735..2953)
                     /gene="infA"
                     /locus_tag="VIR04_07500"
     CDS             complement(2735..2953)
                     /gene="infA"
                     /locus_tag="VIR04_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY9460627.1"
                     /translation="MAKDDVIQMQGEVLENLPNATFRVKLENGHMVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRSK"
     gene            complement(2963..4288)
                     /gene="secY"
                     /locus_tag="VIR04_07505"
     CDS             complement(2963..4288)
                     /gene="secY"
                     /locus_tag="VIR04_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY9460628.1"
                     /translation="MAKAQAKSKSGSDYGDFRRRIIFLVLALVVYRLGTHIPVPGINP
                     DALADLFQQNQSGILGMFNMFSGGALERFSVLALGIMPYISAAIIMQLMAVVVPSLEA
                     IKKEGQSGQRKITQYTRYGTVFLALFQAIGISVALESQPGLVIDPGMLFRFTTIVTLV
                     TGSMFIMWLGEQITERGMGNGISILIFAGIVAGLPSAMGSMLDLVRTDSMSVLSALFI
                     LVLVVAVTYFCVFVESGQRRITVNYAKRQVGNKIYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILLPATITSWFANSPNMRWLGDLAAAITPGQPLYISLYSGLIIFFCFFYTALVFNS
                     RETADNLKKSGAFVPGIRPGGQTARYVDKILMRLTLGGAIYVTLVCLLPELMQARWNV
                     SFYFGGTSLLIIVVVTMDFMAQAQAYLMSHQYDSLLKKANFKGAGLPMR"
     gene            complement(4298..4738)
                     /gene="rplO"
                     /locus_tag="VIR04_07510"
     CDS             complement(4298..4738)
                     /gene="rplO"
                     /locus_tag="VIR04_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY9460629.1"
                     /translation="MSVTQLNTLQPAAGSKRPRRRVGRGIGSGLGKTSGRGHKGQKSR
                     TGGFHKVGFEGGQMPMQRRLPKRGFTPLGQSQYAQLRLSDLQRLPVDEIDVLVLKQAG
                     LIGQGVLYVKVIKAGELSRKVQLKGLNATAGARAAIEAAGGSLA"
     gene            complement(4749..4934)
                     /gene="rpmD"
                     /locus_tag="VIR04_07515"
     CDS             complement(4749..4934)
                     /gene="rpmD"
                     /locus_tag="VIR04_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY9460630.1"
                     /translation="MAQKQIKVTLVRSVIGTKQSHRATIRGLGLTRINSSRVLADTPE
                     VRGMVRKVDYLVTVSQA"
     gene            complement(4938..5459)
                     /gene="rpsE"
                     /locus_tag="VIR04_07520"
     CDS             complement(4938..5459)
                     /gene="rpsE"
                     /locus_tag="VIR04_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY9460631.1"
                     /translation="MAKSQGRHAPDQERDDGMREKMIAVNRVSKVVKGGRTMSFAALT
                     VVGDGDGRIGMGKGKAREVPVAVQKAMEQARHGLIKIALKNGTLYHTVVGKHGASTVL
                     MSPAAEGTGVIAGGPMRAIFDVMGVRNVVAKSLGSSNPYNLVRATLNGLRACSTPAEI
                     AAKRGKSVEEILG"
     gene            complement(5473..5838)
                     /gene="rplR"
                     /locus_tag="VIR04_07525"
     CDS             complement(5473..5838)
                     /gene="rplR"
                     /locus_tag="VIR04_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY9460632.1"
                     /translation="MDKKISRLRRAAATRRKIHELRVHRLSVFRSNLHIYANIISPEG
                     DRVLISASTLEPEVRKELASQKLHGGNVAAAGVVGKRVAEKAKAAGIELVAFDRSGFR
                     YHGRVKALADAAREAGLKF"
     gene            complement(5860..6393)
                     /gene="rplF"
                     /locus_tag="VIR04_07530"
     CDS             complement(5860..6393)
                     /gene="rplF"
                     /locus_tag="VIR04_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY9460633.1"
                     /translation="MSRIAKYPVALPKGVETALAADQITIKGPKGTLTQPLTGDVSVA
                     LEDGHLTFIAASETRHSEAMSGTVRALVHNMVEGVSKGFERRLNLVGVGFRAQIQGDA
                     LKLQLGFSHDILYKMPAGIKIACPTQTEIIVTGADKQVVGQVAAEIRAYRKPEPYKGK
                     GVRYADERVIIKETKKK"
     gene            complement(6404..6799)
                     /gene="rpsH"
                     /locus_tag="VIR04_07535"
     CDS             complement(6404..6799)
                     /gene="rpsH"
                     /locus_tag="VIR04_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY9460634.1"
                     /translation="MSMSDPIADMLTRVRNAQQVNKTSVSMPSSKLKVAIAAVLKDEG
                     YIDGFQVNDDKVKPVLEIVLKYYAGRPVIERIERVSRPGLRIYKGSAAIPQVMNGLGV
                     AIVSTPRGVMTDRKARATGVGGEVLCYVA"
     gene            complement(6811..7116)
                     /gene="rpsN"
                     /locus_tag="VIR04_07540"
     CDS             complement(6811..7116)
                     /gene="rpsN"
                     /locus_tag="VIR04_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY9460635.1"
                     /translation="MAKLSLINRDIKRAKLAAKFAPKRAALKAIIDDQSKSDEERYQA
                     RLQIQQLPRNSNPTRQRNRCVVTGRSRGVYSKFGLTRHKLREMALRGEVPGMTKASW"
     gene            complement(7126..7665)
                     /gene="rplE"
                     /locus_tag="VIR04_07545"
     CDS             complement(7126..7665)
                     /gene="rplE"
                     /locus_tag="VIR04_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY9460636.1"
                     /translation="MARLQEFYRSKVIADLEKKFGYKSIMEVPRITKITLNMGVSEAV
                     ADKKIIEHAVSDLTKIAGQKPVVTKTRKAIAGFKIRENYPIGCMVTLRGQRMYEFLDR
                     LVAVALPRVRDFRGISGRAFDGRGNYNVGVKEQIIFPEIEYDKIDAVRGLNISIITSA
                     KTDEEAKALLTAFSFPFRN"
     gene            complement(7678..7998)
                     /gene="rplX"
                     /locus_tag="VIR04_07550"
     CDS             complement(7678..7998)
                     /gene="rplX"
                     /locus_tag="VIR04_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY9460637.1"
                     /translation="MEKIRKGDEVIVLTGRDKTRRGTVLKRVDADHVLVEGINTLKKH
                     VKPNPMAGTQGGIVDKTMPIHISNVALFNSETGKADRVSIKEVDGRKVRVYRSSGSVV
                     GAKA"
     gene            complement(8009..8377)
                     /gene="rplN"
                     /locus_tag="VIR04_07555"
     CDS             complement(8009..8377)
                     /gene="rplN"
                     /locus_tag="VIR04_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY9460638.1"
                     /translation="MIQMQTTLDVADNTGARKIMCIKVLGGSKRRYAAIGDVIKVTVK
                     DAAPRGRVKKGEVYNAVVVRTAKGVRRKDGSLIRFGGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTDRFMKIVSLAPEVL"
     gene            8676..10103
                     /locus_tag="VIR04_07560"
     CDS             8676..10103
                     /locus_tag="VIR04_07560"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY9460639.1"
                     /translation="MYETLALYIDGEFLSGGGRKTQDVTNPSTLDVLGRLPHATTADL
                     DRALAAAQRAFESWRHSSPMQRSEILRKVGQLSRERAQEIGRNMTLDQGKPLAESVGE
                     IMACSDHADWHAEECRRIYGRVITPRNPAVRQMVLREPVGVCAAFTPWNFPYNQAIRK
                     ICAAIGAGCTIILKGPEDSPSAVMAIARMFHDAGLPPGVLNIVWGVPAEVSDYLIRSP
                     IVRKVSFTGSVPVGKHLASLAGAHMKRVTMELGGHSPVLVFDDADISRAAKQLARFKV
                     RNAGQVCVSPTRFYVHEKVYDEFLEEFIKTLKTVKVGDGLDPETQMGPLAHERRVPVM
                     NKFVENARKRGGKILLGGEPLERTGNYFAPTVVTDLPEDSMLMTEEPFGPIAPIVRFS
                     DTDDVIKRANSLPFGLSSYVFTNSLQTATKVSNGIEAGMVNINHFGSALPETPFGGVK
                     DSGIGSEGGTETFDGYLVTKFVTLI"
     gene            complement(10207..10488)
                     /gene="rpsQ"
                     /locus_tag="VIR04_07565"
     CDS             complement(10207..10488)
                     /gene="rpsQ"
                     /locus_tag="VIR04_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY9460640.1"
                     /translation="MSETENTPVVKSQRTLVGRVVSNKMNKTVVVEVERRVKHPVYGK
                     IVMRSNRYKAHDETNQYNEGDTVEIAEGRPISRDKSWTVVRLVTPAHVI"
     gene            complement(10490..10681)
                     /gene="rpmC"
                     /locus_tag="VIR04_07570"
     CDS             complement(10490..10681)
                     /gene="rpmC"
                     /locus_tag="VIR04_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY9460641.1"
                     /translation="MKASELRSKDATELKSELESLLKAQFNLRMQRATQQLSNTSQLG
                     KVRRDIARTRTVMTETAGK"
     gene            complement(10691..11107)
                     /gene="rplP"
                     /locus_tag="VIR04_07575"
     CDS             complement(10691..11107)
                     /gene="rplP"
                     /locus_tag="VIR04_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY9460642.1"
                     /translation="MLQPARRKYRKEQKGRNTGLATRGAQVSFGEFGLKATGRGRLTA
                     RQIEAARRAINRHVKRGGRIWIRIFPDKPVSHKPAEVRMGNGKGNVEFYVAEIQPGKV
                     LYEMEGVTEELAREAFRLAAAKLPIATTFVTRQMGA"
BASE COUNT         2407 a         3157 c         2971 g         2599 t
ORIGIN      
        1 cgttccaaac cgagcggagg ccagttttcg agtttcatgc caagagtaag accgcgtgcg
       61 gcaagcactt ccttgatttc attcaacgat ttacggccca ggtttggagt tttgagcaat
      121 tcgttttcag tacgctgaat cagatcgcca atgtagtaaa tattttccgc cttgaggcag
      181 ttggcggaac gcaccgtaag ctcaagatcg tcgaccgggc gcaaaagcac tgggtcgatt
      241 tgaggaagct tgccgccatc acgctgaaag tctacatccg ggcctgcgcc ttcgagggcc
      301 gcaaaaaccg aaatctgatc catcaaaatc cgcgccgatt gacgaactgc ctcttcaggt
      361 gaaagaacgc cattggtttc gatatcaagt atgagcttgt ccagatcggt gcgttgttcg
      421 acgcgggcgt tgtcaacggc atagctgacg cggcgcacag ggctgtatga tgcgtcgagc
      481 acgatacggc cgatcgtatg actgcgatcg tcaaccagcg catgcacgtt gccgggaaca
      541 tagccgcggc cctgctcgac cttgatttgc atctcgagct tgcccgcatc ggtcagcgtt
      601 gcaatgacgt gctcggggtt gatgatttcc acatcgtgcg gcaattcaat gtcgccggca
      661 accgccacgc cctgaccctg cttgcgcaac gtcaaagaaa cttcttcacg attatgcagc
      721 ttgaacacga cgcctttgag attcaaaagg atgtcaacca catcttcgcg tacgcccgta
      781 atcgtcgagt actcgtgcac gacaccggtt atctggactt cggtgggcgc gtaacccgcc
      841 atggaggaaa gcaggatgcg acgcagagcg ttgcccagcg tgtggccata gccgcgctcg
      901 aacggctcca tcacgactct tgcgtgattc tttccgacgg ggacaacttc gatagaacgg
      961 ggtttcaaaa aaccttggga cgccatattt gttccttttc agtaccctcg gctcgttaca
     1021 ccgataaggc tgagggacaa gccggactag ccggcaacca caccgccaag cccgcttcca
     1081 gccagatcgg cgaaagcggg cgagtggagg tgccgcagaa ataccgccaa agacctaatt
     1141 agcgggaata cagttcaacg accagcgatt cattgacatc gcgagcgact tcggaacgat
     1201 ccggaatttg cttgaatgtt ccggccatct tggtggcgtc aacgtcgacc cactggggca
     1261 agccctgccc ggtcgccaaa tcaagggact cacgaattcg gagctgacct ttggactttt
     1321 cacgaatcga gacaacatcg ccggctttca cagacatcga ggcgatatcg gccgttctgc
     1381 cattgatttc aaccgtacga tgattgacca actggcgcgc ctcggcacga gtcgcgccaa
     1441 accccatgcg ataaacaacg ttatccaggc gcgattccag caactgaatc aacgtctcgc
     1501 ccgtattgcc gcgacggcgc tcggcttcta caaagtattt gtgaaactgc ttttcaagca
     1561 cgccgtacat gcgcttgagt ttctgttttt cgcgcaactg cacaccgaag tccgatgtac
     1621 gggcgccgga agtgcggccg tgctgacccg gcttggattc gagcttgcat ttcgaatcga
     1681 gcgagcggcg cgagcttttc aaaaataaat ccgtaccctc gcgacgcgag agcttgcatt
     1741 taggtccaat ataacgtgcc aagatgcttc cccttagatg cgacgacgct tgggcggacg
     1801 gcagccgtta tgcggcacgg gcgtgatatc cgagatgctg gaaattttga tccccaatgc
     1861 attcagagca cgcacggacg actcacggcc aggaccgggg cccttgatgc gaacttcgag
     1921 tgttttaatg ccatattcca aagccaggcg tccggccgat tccgccgcca cctgggcagc
     1981 aaatggcgtg gacttgcgcg agcctttgaa accggcgccg cctgacgtcg cccatgacaa
     2041 cgtgttgccc tggcgatcgg tgattgtaat gatcgtgttg ttgaaggacg catggacgtg
     2101 cgcaatgccg tccgaaacgc tcttcttgac ctttttgcgt acgcgggttg cgccgctggt
     2161 gggagctttc gccatcttct aatccttgtt atttcttcag ggaagcagca gcgcgacgcg
     2221 gccctttacg ggtgcgggcg ttggtgcgtg tgcgctggcc gcgcacaggc aagccgcgct
     2281 tatgacgcat gccccggtac gttcccaggt cgaccagacg tttgatcgag agttgtactt
     2341 cgcggcgcaa atcaccttcg actgtgagca cattaatgca ctcacgaata cgctcgagtt
     2401 ccgaatcggt cagatctttc actttcttgg aaaagtcaac gcccgacgct tcgcagattt
     2461 tacgagcacg ggtgcgacca ataccaaaaa tggcggtaag tccgatttcg gcgtgttgat
     2521 gcggcggaat gttaatgcca gcaatacggg ccatgacttt tccttgttaa atccgttacg
     2581 ttttggcgct tagccttgac gttgcttatg acgtgggtcg gtgcaaatga cacgcaccac
     2641 gccgtgacgt ttaatgatct tgcagttgcg gcaaatccgc tttaccgatg ccattacctt
     2701 catggttgac tcctagtttc ctgttagcgc ccgcttattt ggagcggaac acaattctgg
     2761 ctcgtgacag atcatagggg gtgagctcca cagtgacctt gtcgcccggc aaaatccgga
     2821 tgtaatgcat tcgcatcttt cccgaaatat ggccgagcac catatgacca ttttcaagct
     2881 tgacacgaaa agttgcgttg ggaaggtttt caagtacctc gccctgcatt tgtataacgt
     2941 cgtctttcgc cattatctct ggttaccgca ttggcagccc cgcccctttg aagttagcct
     3001 tcttcagaag cgagtcatac tggtgagaca tcaggtaggc ttgtgcctgt gccatgaaat
     3061 ccatggtgac cactacaata atcaggagcg acgtgccgcc gaaataaaac gatacgttcc
     3121 agcgtgcctg catcagttcg ggcaacaaac acaccagcgt gacgtaaatc gcgccgccca
     3181 gagtcagacg cattagaatc ttgtcgacgt agcgtgccgt ttgaccgccc ggccgaatgc
     3241 ctggcacaaa agccccgctc ttcttcagat tatcggctgt ctcacgactg ttaaacacca
     3301 gtgccgtata gaaaaagcag aaaaatataa tcaggccgga atacaagctt atgtacaggg
     3361 gctgtccggg agttattgca gccgcaaggt cgcccagcca gcgcatgttc gggctattcg
     3421 cgaaccagct cgttatggtc gccggcagca aaatgatcga cgaagcgaaa atcggcggaa
     3481 taacgcccga catgttcagt ttgaggggca gatgcgagct ttgcccgcca taaatcttgt
     3541 tgcccacctg acgctttgcg taattgaccg tgatgcggcg ctgcccgctt tcaacaaaga
     3601 cgcagaaata cgtaaccgct accaccagca ccagaatgaa caatgccgac aataccgaca
     3661 tggaatcggt gcgcaccaaa tcaagcatgc tgcccattgc gcttggcaag cccgcgacaa
     3721 tcccggcaaa aatcagaatt gaaataccgt tacccatgcc gcgttcagtg atttgttcac
     3781 ccagccacat aatgaacatg gatcccgtaa ccagcgtgac gatcgtcgtg aagcgaaaca
     3841 gcatgccggg atcgatcacc aggcctggct gcgattcaag ggcgaccgag atgccaatag
     3901 cctggaacaa cgccaaaaac accgtgccat aacgcgtgta ctgggtgatt ttccgttgcc
     3961 ccgactgccc ttctttcttg attgcctcga gtgacggcac caccaccgcc atcaactgca
     4021 tgataatggc ggccgaaata tacggcatga tgcccagagc aagcacggaa aaacgttcca
     4081 gtgccccgcc ggaaaacata ttgaacatgc ccaggatgcc gctttggttt tgctgaaaca
     4141 aatcagccag cgcgtcggga ttgatgcccg gcacggggat gtgtgttcct aaccgataaa
     4201 cgaccagggc gagcaccagg aatatgatac ggcgtctgaa gtcgccgtag tccgatcccg
     4261 atttactctt agcctgagct ttagccaccg ttacccctta agccagcgaa ccgccagcag
     4321 cctcgatggc cgcccgggcc cctgccgttg cattcaggcc tttcaactga accttgcgcg
     4381 acaactcgcc cgccttgata accttgacgt aaagcacgcc ctggccgatg aggcccgcct
     4441 gtttgagcac cagcacatcg atctcgtcga ccggaagacg ctgcaagtcg gacaaacgaa
     4501 gctgcgcgta ctggctttga cccagcggcg tgaagccacg tttgggcaag cggcgttgca
     4561 taggcatttg gccgccttcg aaacccacct tgtggaaacc gcccgtgcgc gacttttgac
     4621 ccttgtgacc acgaccgctg gtcttgccca ggcctgagcc gatgccacgg cccacgcgac
     4681 gccgagggcg tttgctgccg gcggcgggtt gtaatgtatt taactgagtt acagacatct
     4741 cgattccttt aggcctgcga tacggtgaca aggtaatcga ccttgcgaac catgccgcgc
     4801 acctcgggtg tatcggccaa cacgcggctg ctgttgatgc gggtcaggcc caggccgcgg
     4861 attgtggcgc ggtgagactg tttagtgcca atgacagagc gcaccagagt tactttaatt
     4921 tgcttctgtg ccatgtctta ccccaaaatt tcttcaactg atttaccgcg cttagcggcg
     4981 atttcagccg gagtcgagca agcgcgcaaa ccgttaagcg tagcacgaac caggttatac
     5041 ggattgctgg agcccaggct tttcgcgaca acgttgcgta cgcccataac atcaaatata
     5101 gcgcgcattg ggccgccggc tataacgcca gtaccttcgg ccgctggcga cataagcact
     5161 gtcgaagcgc catgcttgcc caccactgtg tgatacaagg tgccgttttt aagagcaatc
     5221 ttgatcaggc cgtgccgcgc ttgttccatt gccttctgta cagcaaccgg aacttcgcga
     5281 gccttgccct tgcccatgcc gatgcggccg tcgccatcac cgaccacggt tagcgcggca
     5341 aagctcatgg tgcggccgcc cttaacgact ttgctgacgc gattgaccgc gatcattttt
     5401 tcgcgcatgc catcatcgcg ttcctgatca ggggcgtgtc tgccttgtga tttagccatt
     5461 gataatcctt gcttagaatt tcaagccggc ttcacgcgcg gcgtcggcca acgctttcac
     5521 gcggccatga taacggaagc ccgaacggtc gaaagccacc agttcaatac cggccgcctt
     5581 cgccttttcc gcgacacgct ttccaaccac gcccgcggcg gcaacgttgc cgccatgcag
     5641 cttttggctg gcgagctctt tgcgaacttc gggttcgagc gtcgaagcgg agatgagcac
     5701 acggtcgcct tccggcgaaa taatgttagc gtaaatgtgc agattcgagc ggaaaaccga
     5761 gaggcgatgc acgcgtagtt cgtgaatttt ccgacgggtc gctgctgcac gacgtaaacg
     5821 ggagattttc ttatccataa ttcgtccttg cctgcgcgcc tatttcttct tggtttcttt
     5881 aatgatgacg cgctcatccg cgtaccgaac gcccttgccc ttgtaaggct cgggcttgcg
     5941 gtaggcgcga atttcggcgg ccacctgacc gacaacctgc ttgtcggcgc ccgtaacgat
     6001 aatttccgtt tgagtcgggc acgcgatctt gatgccagcc ggcattttgt acaggatgtc
     6061 gtgcgaaaaa ccaagttgca acttaagcgc atcgccctgg atctgagcgc gaaaacccac
     6121 gcccaccagg ttcaatcttc gttcaaaccc cttgctgacg ccctcaacca tgttgtgcac
     6181 cagcgcacgc accgtgccgg acatggcttc ggaatggcgg gtttcgctgg cggcaataaa
     6241 cgtgagatgg ccgtcttcaa gcgccacgct cacatcgccg gtaagcggct gcgtcaatgt
     6301 tcccttgggg cctttaatcg taatttgatc tgcggcaagt gccgtttcca cgcctttcgg
     6361 caatgcgacg ggatattttg caatacgtga catatgattc cccttatgcc acgtagcaca
     6421 aaacttcgcc gcccacgcca gtggcgcgag ctttgcggtc agtcatgacg ccacgcggcg
     6481 tcgaaacgat tgccacgccc aggccattca taacctgtgg aatggctgcg ctgcctttat
     6541 aaatacgcag ccctggacgg gatacacgtt cgatgcgttc gatgaccggg cggccagcgt
     6601 aatacttcag tacgatttcc aaaaccggtt tgactttgtc gtcattaacc tggaaaccgt
     6661 cgatatagcc ttcgtctttc agcacagcgg caatagccac tttaagcttc gaggagggca
     6721 tgctaaccga tgtcttgtta acctgttgcg cattacgcac ccgggtcaac atgtcggcga
     6781 tgggatcgct catgctcatt tatatttctc ctaccagctt gctttggtca tgccgggcac
     6841 ctcgccgcgc aatgccatct cgcgcagctt gtgacgagtt aaaccgaatt tgctataaac
     6901 accgcgcgaa cggccggtta caacgcaacg attgcgctga cgagtcgggt tggaattgcg
     6961 cggcaattgt tgaatttgca aacgagcctg atagcgttct tcgtcagatt tcgactggtc
     7021 gtcgataatg gctttcaacg ctgcccgctt gggagcgaat ttcgccgcca gcttggcgcg
     7081 cttgatgtcg cggttgataa gtgagagttt agccacgttc gccccttaat tgcgaaacgg
     7141 gaagctaaag gctgtcaata acgccttggc ttcttcgtcg gtcttggcgg aagtaatgat
     7201 gctgatgttc agcccacgca ccgcgtcgat tttgtcgtat tcgatttcgg ggaaaatgat
     7261 ttgctcttta accccaacgt tgtaattgcc gcgtccatcg aatgcacggc ctgaaatacc
     7321 gcgaaagtcg cgtacacgcg gcaacgccac ggcaaccagg cgatccagga attcgtacat
     7381 gcgctggccg cgcagcgtaa ccatgcagcc aattggataa ttttcgcgga tcttgaagcc
     7441 tgcaatagcc ttgcgcgttt tggtgacgac gggcttttgg cctgcaatct tggtcaaatc
     7501 agagacggcg tgctcgatga ttttcttgtc cgcgaccgct tccgaaacac ccatgttcaa
     7561 ggtgattttg gtaatgcgcg ggacttccat tatgctttta tagccaaact ttttttccag
     7621 gtcggctatg accttactgc ggtaaaactc ttgtaaacga gccatagtgt tcgcccctta
     7681 agccttggcg ccaacgaccg agccgctgga acggtatacg cgcactttgc ggccatcgac
     7741 ttctttgatg ctcacccgat cggccttgcc ggtctcggaa ttgaaaagcg ccacattaga
     7801 gatatgaatg ggcatggttt tatcgacgat gccgccctgc gtgccagcca tgggattggg
     7861 cttgacatgc tttttaagcg tgttgatgcc ctcgaccagc acatggtcgg cgtcgacgcg
     7921 cttgagcacg gtgccgcgcc gggttttgtc gcgaccagtc aatacgatga cctcatcgcc
     7981 cttacgaatt ttttccacgg ttcgcccctt acagtacttc tggcgccagg gatacgatct
     8041 tcatgaaccg gtcagtgcgc aactcgcgcg tgacgggccc gaagatacga gtaccgatgg
     8101 gttccagttt ggcgtttagc aatacagcgg cattgccgcc aaaacgaatc agcgagccgt
     8161 ccttgcgacg cacgccctta gcggttcgaa ccactacagc gttatagact tcgcctttct
     8221 tgacgcgtcc gcgtggagcc gcatctttta ctgtgacctt gatgacgtca ccgattgcgg
     8281 cataacggcg cttcgagccg cccaacacct tgatgcacat aattttacgg gcacctgtgt
     8341 tatcggccac gtctagcgtg gtttgcattt ggatcatgat ttttcctgtt ccaacttagc
     8401 cgcaatgcct tgccaaaaat tggcaaagcc gcacaaccag ttttggtccc gtcagattcg
     8461 tttgtaagcg ccctgtaaat gcgcctgcca ctcaaatcct ggattgaatc ttgctatatt
     8521 tgttgcgatg aatctgttgc gataaatctt tttaatcttg ctatttactt ctctaccagc
     8581 aagtccgtta tattatccta tatttatcga ccgtgcaagc aaacacattg gcaacacaac
     8641 agcgagcagg gaattcaatg cgaggaacac acaatatgta cgaaactttg gcgctttata
     8701 tagatggtga atttctaagc ggcggcgggc gcaagacaca agatgtaacc aacccctcca
     8761 cgctggatgt attggggcgg cttccacacg caactaccgc cgaccttgat cgtgcgctgg
     8821 cggcggcgca gcgcgcattt gagtcctggc gtcacagctc acccatgcag cgctccgaaa
     8881 tcttgcgcaa agtagggcaa ttgtcgcgcg agcgcgctca ggagatcggc cggaatatga
     8941 cgctggacca gggcaagccg ctggccgaat cggtaggcga gatcatggcc tgctcggacc
     9001 acgccgattg gcatgccgaa gaatgccggc gtatttatgg gcgcgttatt acgccgcgca
     9061 accctgctgt gcgccagatg gtgctgcgtg agcccgttgg cgtttgcgcc gcgttcacgc
     9121 cgtggaattt tccctacaat caggcaatac gcaaaatctg cgccgcaatt ggcgcgggct
     9181 gcaccattat tcttaaaggc cccgaggatt ctcccagcgc cgtgatggcc atagcgcgca
     9241 tgtttcacga tgcgggtctg ccgcccggag tgctgaatat tgtatggggc gtgccagccg
     9301 aggtttccga ctatctgatt cgttcgccca tcgtgcgcaa ggtttcgttt acgggttcgg
     9361 tgccggtcgg caagcatctt gcctcattgg caggcgcaca catgaaacgg gtcacaatgg
     9421 aattgggcgg ccactcgccc gttctggttt tcgacgatgc cgatattagt cgcgctgcca
     9481 aacaactggc gcggttcaag gtgcgcaacg ccggccaggt atgtgtatcg cccacgcgtt
     9541 tctatgttca cgaaaaagtc tacgacgaat tcctggaaga attcatcaag acacttaaaa
     9601 ccgttaaagt tggcgatggg ctggatcccg aaacccaaat gggtccgctg gctcatgagc
     9661 gtcgcgttcc cgttatgaac aagtttgtcg aaaatgcgcg caaacgcgga ggcaaaatcc
     9721 ttctgggtgg agaaccgctg gagcgcaccg gcaactattt tgccccaacc gtggtgaccg
     9781 atctgcccga agattccatg ctcatgaccg aagaaccgtt cggaccgatt gcgcctattg
     9841 tgcgattctc ggacactgac gacgtcatca aacgcgctaa ttcgcttccg ttcggcctgt
     9901 cgtcttacgt attcaccaac tcgcttcaga ccgctaccaa ggtatccaac ggaatcgaag
     9961 cgggcatggt caatatcaat cacttcggca gtgcgctgcc cgagacgcca tttggcggcg
    10021 tgaaagacag tggcatcggc agcgaaggcg ggaccgaaac atttgacggt tacctggtga
    10081 ccaaattcgt gacgcttatt taaaccaggc cggcttttca gcgacaacga aaaacgcccc
    10141 gaaaactttc agggcgtttt tcaagtgtag cttgttatcc aagccttgcg gcagggccgt
    10201 aaatgcttag ataacgtgcg caggagtaac caggcgtaca actgtccacg atttatcgcg
    10261 gctgatgggg cggccttccg cgatttccac ggtgtcgcct tcgttgtact gattggtttc
    10321 gtcgtgagcc ttgtagcgat tcgagcgcat gacaattttg ccataaaccg gatgcttcac
    10381 acggcgttca acctcgacca cgacggtctt gttcatttta ttgctgacta ctctgccgac
    10441 caacgtgcgt tggctcttta ctacgggggt gttttcggtt tcgctcattt cactttcctg
    10501 cggtctcagt catcacggtg cgtgtgcgcg cgatatcgcg acgcactttg ccgagttggc
    10561 tggtattgga aagctgctga gtcgcacgct gcatacgcag attgaattgt gctttcagca
    10621 ggctctcgag ctctgactta agttcagtgg cgtctttgga acggagttcg ctggctttca
    10681 taaaatttcc ttaggctccc atttgacgcg ttacaaacgt cgtcgcgatt ggcaacttgg
    10741 cggcggcgag acgaaacgct tctcgggcga gctcttcggt tacgccttcc atctcgtaga
    10801 gcaccttgcc cggctgaatc tcggcaacgt agaattcgac gttacccttg ccgttgccca
    10861 tacggacttc ggccggtttg tgcgaaacag gtttgtcggg aaatatgcgg atccagatgc
    10921 ggccgccgcg tttgacgtga cggttgattg cccggcgtgc agcttcgatc tgacgggcgg
    10981 tcaaacggcc acgacccgtg gccttcaaac cgaactcgcc gaacgaaact tgcgcaccgc
    11041 gtgtcgccag gcccgtgttg cggcccttct gttctttacg atactttctg cgtgcgggtt
    11101 gcaacatgat tattctcctt cgggcgcagg ggcg
//
LOCUS       DATCXU010000334         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_648234,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000334 DATCXU010000000
VERSION     DATCXU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648234"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /gene="cphA"
                     /locus_tag="VIR04_07580"
     CDS             <1..1759
                     /gene="cphA"
                     /locus_tag="VIR04_07580"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY9460643.1"
                     /translation="ERFFSGHDFRLLVVGDNLVAAARRDPPQVIGDGERTIAQLVDLI
                     NADPQRGEGHGMSLTKIRLDDTALTTMSLQGYAPDSIPEKGVLVGLRNNANLSTGGTA
                     TDVTDHVNPELAASAVAAAQMVGLDICGVDLVSDNVYEPLDKQGGCIVEVNAAPGLRM
                     HLTPSFGKGRAVGEAIVNSMFAEGENGRIPLVAVSGTNGKTTTVRLIAHMIACTGKRV
                     GMTNSDGIYVDGRRIDAGDCSGPKSARNVLLHPDVDAAVFETARGGILREGLGFDRCN
                     VAVITNIGAGDHLGMNFINTVEGLAAVKRVIVENVAPDGVAVLNAADPHVAAMAESCP
                     GAVTFFASDRNHPVLATHRAQGHRVICLDAGDIVLTDGAFERRIALDSIPITHRGAIA
                     FQVQNVMAALGAAWALGLDWASIQAALGTFINDAQTAPGRFNLFSYRGATLVADYGHN
                     PDAILALVQAVDTMPARRRTVVISGAGDRRDVDIRRQTEILGNSFDDVILYQDMCQRG
                     RADGEVLALLREGLAGARRTKRVQEIHGEFLAIDTALDVLKEGELCLILIDQVPEALA
                     HIARRIAEQKPVAELSVRA"
     gene            1762..2595
                     /locus_tag="VIR04_07585"
     CDS             1762..2595
                     /locus_tag="VIR04_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEY9460644.1"
                     /translation="MVAGGEPALDRRSATGRVPAIYVISLKSAVQRRAFMRRQLDPLG
                     VRYEFFDAIAGAQSPNHYLFKKYNDRKRIRRRGKGSSLRLSQLGCFASHYLLWEKCLN
                     EDVPIIVLEDDATLLPPFLPFCEHAARFADKYGFVWLQPSRKVANQAGVRLGKIGPFS
                     VRKFAKGFSGTTGYLIRPDVARTLINYCSEWIYPVDNTMDRFYEHNVEAVGMDPVCVV
                     QDDDLESFINVADSGRKRSLIDTLRRECANLVDSSRRIRHNLAFSMKHRRAERSSQSG
                     S"
     gene            2627..3373
                     /locus_tag="VIR04_07590"
     CDS             2627..3373
                     /locus_tag="VIR04_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460645.1"
                     /translation="MVLHKLLPATLFMLLLSGFASGSQAASDTIRVITDPTLPPMEFM
                     QSGQRTGFDIELVEALAKEMGKKVEWTDSDYQGLIPAIVSGRADAAASAIYISDARKK
                     IVDFTDPYLVGGQVVLTKKDGSIKTLKDLDGRKVSVQMGTDSTKVLQSKFPHVKFIEA
                     EKGEVMLDLVNTGRSDAAITGRQAAGLYAHNHPNFAVLPEQITTREYGIAISKKQPEL
                     TKDMNAALARIRNNGDYQKIADKWLKGQVQ"
     gene            3384..4307
                     /gene="ubiA"
                     /locus_tag="VIR04_07595"
     CDS             3384..4307
                     /gene="ubiA"
                     /locus_tag="VIR04_07595"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY9460646.1"
                     /translation="MNDVSGVPDLSDMRPDDWVERWLPHSWGPYARLCRLDRPVGTWL
                     TLLPCLAALIQAAGGWPDLFRLFIFSLGALLMRGVGCTINDIFDRDFDKKVERTRFRP
                     LTSGQISLKNALFFLLGQVAVCALLLLAVNEYSRWLALLLMPIVVVYPLCKRFTYWPQ
                     VVLGIGFNWGMLMAWSDTQNAVPLAALAMWVGAVLWQVGYDSIYAYVDVRDDKMLGLH
                     STAMRFAQNGKKWISGFYAAAVILWVYSGIGMQLALGYYFVLAVIAAHFVWQMRVFDL
                     ARPDRNFMLFRSNMWIGVLVVIAAVAGTVPA"
     gene            4401..>4536
                     /locus_tag="VIR04_07600"
     CDS             4401..>4536
                     /locus_tag="VIR04_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY9460647.1"
                     /translation="MAGQTSQQTSLEFDYIIVGAGSSGCLLANRLSADPKHKVLLLEA
                     G"
BASE COUNT          944 a         1149 c         1410 g         1033 t
ORIGIN      
        1 cgaacgtttt ttctccggcc atgattttcg tttgctggtg gtgggcgata atctggtggc
       61 tgcggcgcgc cgcgacccgc cgcaggtaat tggcgatggc gaacgcacca tcgctcaatt
      121 ggtcgatctg atcaatgccg acccgcagcg cggcgaggga cacggcatgt cgctgaccaa
      181 gatccggctc gatgatactg cgctgacgac aatgtcattg caggggtacg cgccagactc
      241 gattcccgaa aagggcgtcc tggtcgggct tcgcaacaac gccaacttaa gcacgggtgg
      301 aacagcaact gacgtgaccg atcatgtcaa tcccgaactg gcagccagtg ctgtcgcggc
      361 cgcacaaatg gtcgggctgg atatctgtgg cgtggatctg gtcagcgaca atgtctatga
      421 gcccctggac aagcagggcg ggtgcatcgt cgaagttaat gcggcgcccg gattgcgcat
      481 gcatttgacc ccgtcctttg gcaagggccg cgcggttggc gaggccattg tcaatagtat
      541 gttcgccgag ggcgagaacg gccgcattcc gctggtggcg gtttccggca ccaatggcaa
      601 gacgacgaca gtgcgcctga ttgcgcacat gatcgcctgc acgggcaaac gtgtaggaat
      661 gaccaattcc gacggcattt atgtcgacgg ccggcgtatt gatgcgggcg actgcagcgg
      721 gcctaaaagc gcgcgtaatg tgctgttgca cccggatgtc gacgcagccg tattcgaaac
      781 ggcgcggggc ggaatactgc gtgaaggatt ggggttcgat cgttgcaacg tggcggtgat
      841 cacgaatatt ggcgcaggcg atcatcttgg catgaatttc atcaacaccg ttgaagggtt
      901 ggccgccgtc aagcgcgtga ttgtcgagaa tgtggcgccc gatggcgttg cggtgttgaa
      961 cgccgccgac ccgcatgtag cggccatggc cgaaagctgc cctggagcgg tcactttttt
     1021 cgccagcgat cgcaatcacc cggtattggc gacgcaccgt gcgcaagggc atcgcgtgat
     1081 atgtctggac gcgggcgata ttgtcttgac cgatggcgcg ttcgaacgcc gtatcgccct
     1141 cgatagcatc ccgatcaccc atcgcggagc gatcgccttt caggttcaaa acgtcatggc
     1201 ggcattgggc gccgcctggg cgctgggtct ggactgggca agcattcagg ccgcgctcgg
     1261 cacgttcatc aatgacgcgc aaaccgcacc tgggcggttc aatctgtttt cttatcgcgg
     1321 cgcgacgctg gttgccgact atgggcacaa tccagacgcg atccttgcgc tggtgcaggc
     1381 ggtggatacc atgcccgcaa ggcggcgcac ggtggtgatc agcggagccg gcgatcggcg
     1441 cgacgtcgat atccgccgcc agaccgaaat cctcggaaac agtttcgacg acgtaatttt
     1501 atatcaggat atgtgccagc gcggtcgcgc cgatggggaa gtgctggcct tgcttcggga
     1561 aggactggcg ggcgcccggc gcacaaagcg ggtgcaagaa atccacggtg aatttttggc
     1621 catcgatacc gcgcttgatg tgttaaaaga aggcgaacta tgcctgattc tgattgacca
     1681 ggtgccagag gcgctagcgc acattgcacg gcgcattgcg gagcaaaagc ctgtggcaga
     1741 attatcggtc cgcgcttgac catggtcgcg ggtggcgagc ccgctcttga caggcgatcc
     1801 gcaacggggc gagtgccggc tatttatgtg attagcctga aatcggcggt gcagcggcgc
     1861 gcattcatgc gccgtcagct cgacccgttg ggtgtgcgct acgaattttt cgatgccatc
     1921 gctggcgcgc aaagccccaa tcattatttg tttaagaaat acaatgaccg gaagcgaatt
     1981 cggcgtcgtg gcaaaggttc atcgctgcgc ttgtcgcaac tagggtgttt cgccagccat
     2041 tacttgttat gggaaaagtg tctgaacgag gacgtcccca tcattgttct ggaagacgat
     2101 gccacgttgt tgccgccctt cctgccgttc tgcgagcatg ccgcccgatt cgccgataaa
     2161 tatggttttg tctggttgca gcctagcaga aaagtcgcca atcaagccgg cgtgcggttg
     2221 ggaaagatcg gcccgttttc tgtccgaaaa tttgccaaag gctttagcgg gacgacaggg
     2281 tacttgataa ggcctgatgt tgcccgcacg ctcataaatt actgctcgga gtggatatat
     2341 cccgtggaca acaccatgga ccggttctat gagcataacg tggaggccgt tgggatggac
     2401 ccggtatgcg ttgtccagga tgacgatttg gagtctttca taaacgtggc ggactcgggg
     2461 cgtaaacgat cactgatcga cacgctgcgc agagaatgcg ctaacctagt cgactctagt
     2521 cgaaggatta gacataatct ggcgttttcc atgaagcaca ggcgggcaga gcggtcgtcg
     2581 cagtccggat cgtagacgct cggctttttc gaaaccggga ggtgtcatgg tattgcataa
     2641 attattgccg gccacgctgt ttatgctgct tttaagcggt tttgcgtccg gcagccaggc
     2701 cgcgtccgat acgattcgcg tcattaccga ccccaccttg ccgcctatgg agtttatgca
     2761 gtctggccaa cgcacgggtt ttgacattga gcttgtcgaa gcactggcca aagaaatggg
     2821 caagaaagtg gaatggacag acagcgatta tcaaggtttg atcccggcga tcgtgtcggg
     2881 ccgggccgat gcggctgcct cggcgattta catcagcgat gcgcgcaaga agatagtcga
     2941 tttcaccgat ccgtatctgg ttggcggcca agtcgtgctg accaagaaag acggcagtat
     3001 taaaacattg aaagatctgg acggccgaaa ggtgtccgtg cagatgggca ccgattccac
     3061 caaggttttg cagagcaaat ttcctcatgt gaaattcatt gaggcggaaa agggcgaggt
     3121 gatgcttgac ctggtgaata ccggccgcag cgacgcggcg ataacgggga ggcaagcggc
     3181 gggactctat gcgcacaatc atcctaattt cgcggttctg cccgaacaga tcacgacacg
     3241 ggaatacggc attgccatta gcaagaagca gcctgaactc accaaagaca tgaatgcagc
     3301 gctggcgcgt atcagaaaca atggcgacta tcagaagatc gccgataaat ggcttaaagg
     3361 gcaggttcag tgagtgcgtt gtgatgaatg atgtttcggg tgttccagat ttgtcggaca
     3421 tgcgtcccga cgattgggtc gaacgttggt tgccgcattc atgggggcct tatgcccggt
     3481 tgtgccgtct tgaccgtcct gtcggcacat ggttgacgtt attgccgtgt cttgccgcgc
     3541 tgattcaggc cgctggcgga tggcccgatc tatttcgctt gtttattttt tcgctgggtg
     3601 cattgctcat gcgaggcgtc ggctgcacca tcaacgacat ctttgatcgg gattttgata
     3661 aaaaggtgga acgcacgcgg tttcggccgc tcacaagcgg acaaatcagt ttaaaaaatg
     3721 cgctgttttt tttgctgggg caagtggcgg tatgcgcatt attgttattg gcggtgaatg
     3781 aatacagccg gtggctggcg ctattgctga tgccgattgt ggttgtttat cccttgtgca
     3841 agcgattcac ctactggccg caagtcgtcc tgggcattgg ctttaactgg ggcatgctga
     3901 tggcgtggtc cgatacgcag aatgcggtgc cgcttgcggc gctcgccatg tgggtcggcg
     3961 cggtgctgtg gcaggttggc tacgattcga tttatgccta cgtggacgtg cgcgacgata
     4021 aaatgctggg cttgcattcc accgcaatgc gattcgcgca aaatggcaaa aaatggatta
     4081 gcggatttta tgccgcggcc gtgattctgt gggtttatag cgggattggc atgcaactgg
     4141 ccctcgggta ctactttgtt ttggcggtta ttgcggcgca tttcgtctgg caaatgcgcg
     4201 tattcgatct cgccaggcct gaccgcaatt tcatgctttt ccgatccaat atgtggatcg
     4261 gcgtattggt ggtcattgcg gcggtggcag gcactgtgcc ggcctgatgg gcctgcaatg
     4321 atcggccctg acccaatggg attaaaataa taaaccgtgc aatggcacga aagagcacag
     4381 aaaaatcagg agacacagtc atggcggggc agacgtcgca gcaaaccagt cttgagtttg
     4441 attacattat tgtgggtgcg ggatcgtcgg gatgcttgtt ggcaaatcgc ctgtccgcag
     4501 acccgaaaca taaggtgttg ttgcttgagg ccggcg
//
LOCUS       DATCXU010000335         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1155833,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000335 DATCXU010000000
VERSION     DATCXU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155833"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..946
                     /locus_tag="VIR04_07605"
     CDS             116..946
                     /locus_tag="VIR04_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY9460648.1"
                     /translation="MSNTTAPGTAAAPFELVGSVWLRSGDQNWGSQRRFALLAAIGAE
                     GSISAAAKKVGLSYKAAWDAVDAMNNLAGEPLVLRVTGGQRGGGARLTARAVELIELY
                     RDVSREHERFMAQLAKVNTRSAHNLELIQNMMMQTSARNKLAGVVEQIKPGAVNSEVI
                     LGIGHDRHIVASITEESVRNLELRVGRRALAFIKASSVMVGLPGEGLELSARNQLQGH
                     VNRIVRGTVSAEVCIEIAQGSVIAAVISDESVNVLMLKEGDSAIAIFKASSVMLGVFD
                     "
     gene            971..>3252
                     /locus_tag="VIR04_07610"
     CDS             971..>3252
                     /locus_tag="VIR04_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460649.1"
                     /translation="MLAHVVLKPMIESFAPVSMADLQNRDSASTLVLTVNNRQARRII
                     AQLSAGLTQARRVMALPDILPLSAWLRRAGNSLSLRSEAELASHTIDAFGARILWQRV
                     IAEIEADRALLDVAQAARLAMEADRLMSEWRLAVRPEHETSDYQRFCIWRDQYRAKLL
                     AMDAEDGSLGAERVIDAIRNSDADFSFDTVVLAGFNELSPRFSNVLDVMRQRGVQVLV
                     LQPEQFPACRVQRIAAPDPDSEWRLAAQWAAARLRESPEGHYAIVAARLEADAALAHR
                     ALREALRTDDFVMPYNVAVARPLAEWPLVRAAIAWLNVLHAFSQDKSCRPAMAGEALL
                     AGACAGHSRESSGRAAIDAMWRKRAEISVSAEAFSDRLSHMAPELARAWKLSLADCEH
                     QQDTAAIDVWAGVFRRVLQSLGFPGQSALDSHAYQVLDAFDQTLDILGRQTTAIGRVG
                     FGAAILLLTQLARETPFQPARDPAARLDVLGILESEGGRWDGVWVLGLTDEILPAVPK
                     PNPFIPLITLRQANAPRATPERELQWAQSMYDALLRCAPVAWVSHAQYEGERELRPSP
                     YIAALPAIMQAQAPSSGQGPCQLETLIDEQGPPLLAGVATHGGIGVIDTQSRNPLWAF
                     VKYRLGASEMMDYADVADQNARGLFLHRAIELVWLMARDQVTLQRIHDQGGLGALVEQ
                     SVGQAAQEYLKDYGKVLTRLETTRGIILLNDWLMRELGRKPFIVQDLEKTYLWSYGSL
                     ELTVRLDRVDEFADGRLAVI"
BASE COUNT          588 a          861 c         1073 g          730 t
ORIGIN      
        1 agccgatgtg caggcattgg ccgatcaggt tttccggata cgcgacggaa agatcgccgg
       61 ctgtgccggc ccggcgagcc ggtttgaggt cgccgaaggg taaactgggt ttgccatgtc
      121 aaacacaacc gctccgggaa ccgccgccgc accattcgag cttgtcggct cggtctggct
      181 gcgttcgggc gaccaaaatt ggggcagcca aaggcgtttc gcattgctgg ccgcgatcgg
      241 cgccgaggga tcaatcagcg ccgcggcaaa aaaggtgggc ttaagctaca aggctgcctg
      301 ggatgcggtt gacgccatga ataatctggc cggcgaaccc ctggtgctgc gtgttaccgg
      361 cggccagcgt ggcggcggcg cgcgtttgac cgcgcgcgct gtcgagctga ttgagcttta
      421 tcgcgatgtt agccgcgagc acgagcgttt tatggcgcaa ctggccaaag tcaatacgcg
      481 ctcggctcat aacttggagc ttattcaaaa catgatgatg cagacatcgg cgcgcaataa
      541 gttggcagga gtcgttgaac aaatcaagcc cggcgcggtt aacagcgagg tgattctcgg
      601 gataggccac gatcggcata ttgtcgcttc cattacggaa gaaagcgtgc gaaaccttga
      661 attgcgcgtg ggcaggcgcg ctctggcgtt catcaaagcc tcttcggtaa tggtcggcct
      721 gcctggcgag ggtcttgaat tatcggcccg caatcaattg caagggcacg tcaatcgcat
      781 tgtcaggggg acggtcagcg ccgaggtttg cattgagatc gcgcaaggca gcgtcatcgc
      841 cgccgtgatc agcgatgaaa gtgtcaatgt gctgatgttg aaagaaggcg actccgcgat
      901 tgccattttc aaggcgtcca gtgtgatgct gggcgttttt gattgaacgt tctctgcgtc
      961 gttagaatac atgcttgctc atgttgtatt gaaacccatg attgaatcgt tcgctccggt
     1021 gtcgatggct gaccttcaga atagggatag cgctagcacc ctggttctga ccgtgaataa
     1081 tcgtcaggct cgccgaatca ttgcacaatt gtcggccgga ttgacgcagg cgcgccgggt
     1141 tatggcgctt cccgatattc tgcccttgtc ggcctggttg cggcgggctg gcaattcttt
     1201 gtcgcttcgt tcggaagcgg agctggcgtc gcacacaatt gacgcatttg gcgcgcggat
     1261 tctttggcag cgggtgatcg ccgagatcga ggccgacagg gcgttgcttg acgtggcgca
     1321 agcggcacgt ttggcgatgg aggcggatcg cctgatgtcc gagtggcgcc tggccgtgcg
     1381 ccccgagcat gaaacaagcg actatcagcg tttttgtatc tggcgcgatc agtatcgcgc
     1441 caagttgctg gcaatggacg ccgaagacgg cagcttgggc gccgaacggg tcattgacgc
     1501 catccggaac agtgatgcgg atttttcatt cgacacggtt gtactggccg gctttaatga
     1561 actgtcgccg cgtttctcta acgtacttga tgtaatgcgc cagcgcggtg tgcaggtgct
     1621 tgttttgcaa ccggaacaat ttcccgcctg tcgcgttcag cgcatcgctg cgcctgatcc
     1681 tgactccgag tggcgattgg cggcgcagtg ggcggcggcg cggttgcgcg aaagccccga
     1741 agggcattat gcgattgttg cagcgcgcct ggaggccgat gcggcactgg cgcatcgcgc
     1801 attgcgcgag gctttgcgaa ctgatgattt tgtcatgccg tataacgtgg cggtggcgcg
     1861 gccgttggcg gaatggccgc tggtgagggc cgccattgcc tggctcaatg tgttgcacgc
     1921 attttcgcag gacaagtcgt gccgcccggc gatggcgggc gaagcactgt tggcgggcgc
     1981 gtgcgccggt cattctcgcg agtcttcggg gcgtgcggca attgatgcaa tgtggcgcaa
     2041 acgggccgaa atcagcgttt cggccgaagc gttttctgac cgtctgtctc atatggcgcc
     2101 cgaacttgcg cgtgcctgga agctgtcttt ggcggattgt gaacaccagc aggacacggc
     2161 cgccatagac gtttgggcgg gggtcttcag gcgtgtgctg caaagcctgg gtttccccgg
     2221 gcaatctgcg ctggatagcc atgcctatca ggtactggac gccttcgacc agacacttga
     2281 tattcttggc cggcaaacga cggcgatcgg ccgcgtcggt ttcggagccg caattcttct
     2341 gctgacccag cttgcccgcg aaacgccgtt tcagcccgcc cgcgatccgg ccgcgcggct
     2401 ggatgtgctg ggtattctgg agtccgaggg ggggcgttgg gacggcgtct gggtgctggg
     2461 tttgaccgat gagattttgc ctgctgttcc caaacccaat ccgtttattc ccttgatcac
     2521 gttgcggcag gccaacgccc cgcgggcaac gcctgaacgg gagttgcagt gggcgcagtc
     2581 catgtatgac gccttgttgc gttgcgcgcc cgtggcgtgg gtcagtcacg ctcagtacga
     2641 gggcgagcgt gaattgcggc ccagccctta tatcgcggcg ttgcccgcga ttatgcaggc
     2701 gcaggcgcct tcttccgggc aaggtccatg ccagcttgaa actctgatcg acgagcaggg
     2761 gccgcctttg ttggccggag tggcgacgca tggtggtatc ggagttatcg acactcagtc
     2821 gcgcaatccc ttatgggcgt tcgtcaagta ccgtttgggt gcgagcgaga tgatggacta
     2881 tgccgatgtc gccgatcaaa atgcgcgtgg tttgtttttg catcgcgcta ttgagttggt
     2941 ctggctgatg gcgcgcgatc aggttacttt gcagcgcata cacgaccagg gcggactggg
     3001 cgcgctggta gagcaatcgg tggggcaggc agcccaggaa tatctgaagg actacggcaa
     3061 ggtcctgact cggcttgaaa cgaccagagg cataattttg ctgaatgact ggctgatgcg
     3121 cgagctgggg cgcaaaccat ttattgttca ggatctcgag aaaacatacc tttggtccta
     3181 cggttctctg gagttgacgg tgcggctgga tcgtgttgat gaattcgccg acggacgcct
     3241 ggccgtgatt ga
//
LOCUS       DATCXU010000336         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_14329,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000336 DATCXU010000000
VERSION     DATCXU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14329"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VIR04_07615"
     CDS             complement(<1..1302)
                     /locus_tag="VIR04_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460650.1"
                     /translation="MIRATGLTLRRGPKVLLNEADFVVNPGERVGIVGKNGAGKSTLF
                     ALLQGKLEADAGSLSIPAHWRIASVTQEISGNDQPAREFVIDGDTHLRALQAERAATP
                     DNKGNRIAELESALIEADAWSAPSRAEQLLAGLGFTPEQWLDPVKSFSGGWQMRLALA
                     RALMAPSELLLLDEPTNHLDLDAMIWLERWLGAYQGTVMLISHDTEFLDGVAKSILHF
                     DHAKLVRYRGGYQDFQTQRAEHLRQTSQAWDRQTREAARLQSFIDRFKAKATKAKQAQ
                     SRVKALARMQVMAPLHAEAGIDIRIPTPKSVPDPLLALDKVSTGYLLSDGRVKTILRD
                     VQLTVRAGSRIGVLGVNGAGKSTLIKTLANELAPLHGDRRASKGLEIGYFAQHQLDIL
                     DLESTPLQHLMRVAPRTREQELRNYLGGFGFSGDAVTSKIAP"
     gene            complement(1299..2195)
                     /gene="prmB"
                     /locus_tag="VIR04_07620"
     CDS             complement(1299..2195)
                     /gene="prmB"
                     /locus_tag="VIR04_07620"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY9460651.1"
                     /translation="MDDAACHELHTLRDLLRFAVSQFNSSGLAFGHGSDNAWDEAVYL
                     LLHTLHLPLDTLDPFLDAHVLPHERERFLKLVDRRMTERLPAAYLTGEAWLQGQHFTV
                     DQRVIVPRSPISELLVHNLAPWVVKPEKVNAVLDLCTGSGCLAVLAALAFDNAHVDAS
                     DLSERALEVARLNVENFGLGDRVAAHHSDLFDNLPARAYDVIVCNPPYVNDQSMEALP
                     AEYKHEPTMALAGGQDGMDLIRRILQDAPDFMAQDGILILEIGHEYENFIAAFPDLDP
                     VWLSTENTENQILLFNRKQLTS"
     gene            complement(2216..3358)
                     /gene="dapE"
                     /locus_tag="VIR04_07625"
     CDS             complement(2216..3358)
                     /gene="dapE"
                     /locus_tag="VIR04_07625"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY9460652.1"
                     /translation="MPDSAVLQLGKDLIACKSVTPADGGCHAILTGLLSQSGFTCENM
                     IFGDVSNLWARRGTTAPLFIFAGHIDVVPTGPLDQWTSDPFTPTERDGKLYGRGASDM
                     KSSVAAFVTAAREFVASHPGHNGSIGLLITSDEEGPALDGTVKVCAALAARNEKPDYC
                     IVGEPTSVDLLGDTVKNGRRGSLSGKLTVKGKQGHVAYPHLARNPIHLLAPALTQLAQ
                     TQWDQGNEHFPPTTFQVSNIHAGTGVSNVIPGAAVLDFNFRFSTASTPESLKERVLAI
                     LNRHQLEFDLEWTLGGEPFLTAGGDLGHALQRAIKDETGISTQSSTTGGTSDGRFIAK
                     ICPQVIEFGPINASIHQINEHIDISALEPLKNIYRKTLENLLLGQA"
     gene            complement(3358..4194)
                     /gene="dapD"
                     /locus_tag="VIR04_07630"
     CDS             complement(3358..4194)
                     /gene="dapD"
                     /locus_tag="VIR04_07630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY9460653.1"
                     /translation="MTSNLQTVIEQAWDNRAQILSGGAPSAGDSTAVREAVQATIDAL
                     DAGRLRVAQKMDGKWVVHQWVKKAVLLSFRLQANEIMGQAPMQFYDKVPLKFGGYSHA
                     DFVDGGFRVAPPAVARKGAFIGKNVILMPSYVNIGAYIDEGTMVDTWATVGSCAQIGK
                     NVHLSGGVGIGGVLEPLQGNPTIIEDNCFIGARSEIVEGVVVEENSVLAMGVFLSQST
                     KIYDRETRSISYGRVPSGSVVVPGSLPSEDGSHSLACAVIVKRVDAQTRSKTSINELL
                     RA"
     gene            complement(4201..>4591)
                     /locus_tag="VIR04_07635"
     CDS             complement(4201..>4591)
                     /locus_tag="VIR04_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY9460654.1"
                     /translation="FLLYRTYHGGAMSGVVSQASIAAWNDETHVVENRRLYREKFEAV
                     VPLLKPALNVEWPQASFYLWAPTPYDDVEFTRNLYSKTAVTVLPGSFLARQAGSVNPG
                     ANHIRIALVAPKEQCVEAVQRIAQFLL"
BASE COUNT          939 a         1288 c         1346 g         1018 t
ORIGIN      
        1 tggagcaatt ttgcttgtga ctgcgtcgcc gctgaaaccg aagccgccca ggtaattacg
       61 cagttcctgt tctcgggttc ggggcgcaac gcgcattaaa tgttgcagtg gcgtgctttc
      121 aaggtccagt atatcgagct gatgctgtgc aaagtagccg atttccagac cctttgaagc
      181 gcgtctgtcg ccgtgtagcg gcgccagttc gttggccagc gtcttgatca gcgtgctctt
      241 gccagcgcca ttcacgccca gcacgccaat acggctgcct gcgcgcacgg tcaactggac
      301 gtcgcgcaaa atagttttga cgcggccgtc ggacaggaga tagccggtcg ataccttatc
      361 aagagccagc agtggatcgg gcacgctttt aggcgttgga attcggatgt cgattcccgc
      421 ttcagcatgc aagggggcca tgacctgcat gcgcgccagc gccttgaccc ggctttgcgc
      481 ctgctttgcc ttggttgcct tagccttgaa gcgatcgata aagctttgaa gacgagccgc
      541 ttcacgggtt tgccggtccc atgcctgaga ggtctggcgc aggtgttcgg cccgctgtgt
      601 ttgaaaatcc tggtaaccac cgcgatatcg caccagtttt gcatgatcga agtggagaat
      661 tgattttgcc acgccatcga gaaattcggt gtcgtgggaa atcagcataa ccgtgccctg
      721 atatgccccc agccagcgct caagccagat catggcatcc aggtcaaggt gattggtggg
      781 ctcgtcaagc aataggagtt cggaaggcgc catcagtgcg cgggccagcg ccaggcgcat
      841 ttgccatcca cccgaaaagc tcttgaccgg gtccagccat tgctcgggtg tgaagcccag
      901 accggccagc agttgctccg cgcgcgatgg cgcgctccag gcatcggctt cgatcagtgc
      961 cgattccagt tcggcgatac ggttgccctt gttgtcaggc gtggccgcgc gttcggcctg
     1021 aagggctcgc aaatgagtgt cgccgtcgat gacgaattcc cgtgcgggtt gatcgttgcc
     1081 gctgatttcc tgagtgacgc tggctattcg ccaatgagcc gggatggaca gcgatcccgc
     1141 atccgcttcg agcttgccct gcaaaagtgc gaacagcgtc gatttgccag cgccattctt
     1201 gccaacaatg ccgacgcgct cgccggggtt gacgacaaag tccgcttcat tgagcaacac
     1261 cttgggcccg cggcgcaagg tcagacctgt ggcgcggatc acgatgtcaa ctgctttcta
     1321 ttgaacaaca aaatttgatt ttccgtattt tcagtactta accatacagg gtccagatcg
     1381 gggaatgcgg cgatgaaatt ttcatattcg tgaccgattt ccaaaatcag aatgccgtct
     1441 tgcgccatga agtccggcgc atcttgcaag atgcgccgga tcagatccat gccatcttga
     1501 ccgcccgcca gcgccatagt gggttcatgt ttgtattcag ccggcaacgc ttccatggac
     1561 tggtcgttga cgtagggcgg gttgcaaacg atcacgtcgt atgcgcgcgc gggcaaattg
     1621 tcgaacaggt cgctgtggtg tgcggcaacg cgatcaccca gtccgaaatt ttcaacatta
     1681 agcctggcga cctcgagcgc gcgttcggat aaatcgctgg catcgacgtg cgcgttatcg
     1741 aatgccagcg ccgccagcac tgccagacag ccggatccgg tgcacagatc cagaacggca
     1801 ttgacctttt cgggtttgac tacccatggg gccaagttat gcaccagcag ttcggatata
     1861 ggcgatcgcg gcacaatgac ccgctgatcc accgtgaaat gctgaccctg cagccaggcc
     1921 tcgcccgtaa ggtaggcggc cggcaggcgt tcagtcatgc gccgatcgac cagcttcagg
     1981 aatcgttcgc gttcgtgcgg caggacgtgc gcatccagaa acggatccag cgtgtccagc
     2041 ggcaaatgca aagtgtgcag cagtaaatag accgcttcat cccaggcgtt gtcgctgccg
     2101 tggccaaagg ccaggcccga cgaattgaac tgactgacgg caaagcgcag caggtcgcgc
     2161 agcgtatgta attcgtggca agcagcatcg tccatggcgg ctccagtgat aatgattacg
     2221 cctggccgag caacaggttt tcgagtgttt ttcggtagat gttcttcaag ggttccaggg
     2281 cactgatgtc gatatgttca ttgatctgat gaatgctggc gttgatggga ccgaactcga
     2341 taacctgagg gcaaatcttc gcgatgaaac gcccgtcgga cgtgccaccc gtggtcgaag
     2401 actgggttga gatacctgtt tcgtctttaa tggcgcgctg caaggcgtgg ccaagatctc
     2461 caccggctgt caggaaaggt tcgccgccca gcgtccattc cagatcgaac tcaagctggt
     2521 ggcgattcag tatggcaaga acccgttcct ttagggactc tggcgtgctg gcggtggaaa
     2581 atcggaagtt gaaatcaagc acggcggcgc caggtatgac gttggacacg ccggttccag
     2641 cgtgaatatt cgaaacctga aaggtggtgg gagggaaatg ttcgttgccc tgatcccact
     2701 gggtttgagc caactgcgtc agcgctggcg ccagcaggtg gatggggttg cgcgccaagt
     2761 gcggataggc gacatgacct tgtttgcctt tgacggtcaa ttttccggac agcgacccgc
     2821 ggcggccgtt ttttacggta tcgcccagca gatcgacgga ggtcggttcg ccgacgatgc
     2881 agtaatcggg tttttcattg cgcgccgcca gcgccgcgca aaccttgacc gtgccatcga
     2941 gcgctggtcc ttcctcgtcc gatgtgatca gcaaaccgat tgaaccgttg tgcccgggat
     3001 gactcgccac gaactccctg gcggccgtca caaaggcggc aaccgagctt ttcatatcgg
     3061 acgcgccgcg accataaagt ttgccgtcgc gttcagtcgg ggtgaatggg tcgcttgtcc
     3121 actgatccag tggaccagtg gggacgacgt cgatgtggcc ggcaaagata aacagaggcg
     3181 ctgttgtgcc ccggcgtgcc cacaaattgg acacatcgcc aaaaatcatg ttctcgcagg
     3241 tgaaacctga ctggctcaga agccccgtca gtatggcgtg acagccgcca tcggcagggg
     3301 tcacggactt gcatgcgatc agatctttgc caagttgtaa aacggctgaa tcaggcatca
     3361 ggctctcaat agttcgttaa tgctagtttt tgatcgggtc tgggcatcga cccgtttgac
     3421 gatcaccgcg cacgccaggc tgtgcgagcc atcttcggag ggcaatgatc cgggcacgac
     3481 cactgagccg gaaggcaccc ggccgtaact gatggagcgt gtctcacggt catagatttt
     3541 tgtactctgg gaaagaaaca cgcccatggc caataccgag ttttcttcca ccacgacgcc
     3601 ttcgacgatt tcggatcgcg cgccgatgaa gcagttgtcc tcgatgatgg ttgggttgcc
     3661 ttgcaacggc tcaagcacgc cgccgattcc caccccgcct gacaaatgca cgttctttcc
     3721 gatttgcgcg catgaaccca cggtggccca cgtgtcgacc attgtccctt cgtcgatgta
     3781 ggcgccgata ttaacgtacg agggcatcag tatgacgttc ttgccaatga acgcgccttt
     3841 gcgagccacc gcgggcggcg ctacacgaaa gccgccatcg acaaaatccg cgtggctgta
     3901 gccgccgaat ttgagcggca ccttatcata aaactgcatg ggtgcctggc ccatgatttc
     3961 gttggcctgc aggcgaaacg acaagagcac tgcctttttg acccattggt gcacgaccca
     4021 tttgccatcc attttctggg caacgcgcaa ccggcccgcg tcaagcgcgt ctatcgtggc
     4081 ttgcacggct tcgcggactg cggtgctgtc cccggccgac ggagcgccgc ccgacagaat
     4141 ttgggcgcga ttgtcccagg cttgttcaat aacagtttgc agatttgatg tcatggttgt
     4201 ttaaagtaga aattgggcta tgcgttgcac cgcttcgacg cattgttctt tgggcgccac
     4261 gagggcgatt cttatatggt tggcgccagg gttcaccgag ccggcctggc gcgccagaaa
     4321 gcttcccggc aatacggtga ccgctgtttt actgtacaaa ttgcgcgtga attcaacgtc
     4381 atcgtatggc gtgggagccc acaaatagaa tgaggcttgc ggccattcca catttagcgc
     4441 gggctttagc agtgggacga ctgcttcaaa tttttcacga tacaggcggc gattttctac
     4501 gacgtgggtc tcgtcattcc aggctgctat gctggcttgc gagactacgc cgctcatcgc
     4561 gccgccgtga tacgtgcgat aaagcaaaaa g
//
LOCUS       DATCXU010000337         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_886696,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000337 DATCXU010000000
VERSION     DATCXU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886696"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VIR04_07640"
     CDS             <1..1107
                     /locus_tag="VIR04_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460655.1"
                     /translation="SGSAATYGKFAQQGMDLAMKEINEKGGVLGRPLSAQIEDGQGKA
                     AVAIQAARKLVYQDKADILMGLDSSGVAQGLAPLVPDLDKPFIITHAATPDVTGKLCN
                     SHTFRISVNVNQNMKGAAEIAAKTGVKKWTTIGPDYAFGHQTWEYFGKYLKEIKPDVV
                     LMKETAFPRFGAEDFTPFINSVMENKPEGVMISLWGGDLVNFVRQATDRGFFKQGFQI
                     VVPVGAATEVLTALGEQMPEGLWLGTRYWYAAFDNDMNKHFVAAYEKSYGVPPSYNAE
                     GAYAAVYAYKAAAEKAGSVDTKAIIAALTGLTFNAPTGPLTIRKGDNQALIGPTWGLS
                     GPMNAKDKIRSLTQVKVFDGNAVTPSVADTGCKL"
     gene            1183..2055
                     /locus_tag="VIR04_07645"
     CDS             1183..2055
                     /locus_tag="VIR04_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460656.1"
                     /translation="MTGFGMALLNSLDIGLLLFVIAVGLNIIFGVLNVINFAHGALYM
                     VGAYLAFTCMNLMLMPFWLTLLIAPLGVAILAVIVDRVMLRFIYQRDISDSLLLTFAL
                     LLILNDAVRLIWGTGIHVVEPPVLLRGTAHLPGGLAYPVYSGFVVVIGLCILCALWLL
                     FNRTRIGKIMRAAALDREMSQALCINTKLVITSVFAFGAWLAGVGGVIAAPMRAIDPG
                     MGDKIIIESFIVVVIGGLGSFPGALLGAIILGLIYGFGGRYLPEVNLMLPFVGMAIVL
                     LLKPRGLMGKRAAS"
BASE COUNT          434 a          530 c          639 g          502 t
ORIGIN      
        1 tcgggcagcg cagccacata tggcaagttt gcccagcaag gcatggatct ggctatgaaa
       61 gagattaacg agaagggcgg cgtgctgggc cggccgctgt cggcgcaaat cgaggatggc
      121 cagggcaagg ccgctgtcgc catacaagcc gcgcgcaaac tggtgtatca ggacaaagcc
      181 gacatcctga tggggctcga tagctcgggc gttgcgcaag gtcttgcgcc gctggtgccc
      241 gacctggaca agccctttat cattacgcac gccgccacgc ctgatgtcac aggcaagcta
      301 tgcaatagcc acaccttccg gattagcgtg aacgttaatc agaacatgaa aggcgcggcc
      361 gaaattgccg ccaaaacggg tgtgaaaaaa tggacgacca tcggcccgga ttacgcgttt
      421 ggccatcaga catgggaata ttttggcaaa tatctgaaag agatcaagcc tgatgtggtg
      481 ctgatgaaag aaaccgcatt cccgcgtttc ggcgccgagg acttcacccc gtttatcaat
      541 agcgtcatgg aaaacaaacc agagggtgtc atgatttcgc tatggggcgg cgacctggtt
      601 aattttgtgc ggcaagccac tgaccgcggc ttcttcaagc aggggttcca gattgtggtg
      661 cctgtgggcg cagccaccga agtgctgacg gccttgggcg agcagatgcc cgaagggttg
      721 tggctgggca cgcgctattg gtacgccgcg ttcgataacg acatgaacaa gcactttgtc
      781 gccgcttatg aaaagagtta tggagttccc cccagctata acgccgaagg cgcgtatgcg
      841 gccgtgtacg catataaggc agcggccgag aaggccggca gtgtcgacac caaggcgatt
      901 atcgccgccc tgacgggttt gaccttcaat gcgccgaccg ggccgctgac catacgcaaa
      961 ggggataacc aggcattgat cggaccaacc tggggtcttt ccggccccat gaacgccaaa
     1021 gacaaaattc gttccttaac gcaagtaaag gtcttcgatg gcaatgcggt gactccgtcg
     1081 gtggccgata caggttgcaa gctctagctc attcccggct tttttaacaa ggtcacctcc
     1141 cggctaatcc ccgggaggtg aaaaataatg gaagggggcg ccatgacggg cttcggaatg
     1201 gctttactca atagtcttga tataggcttg ctgctgttcg tgatagcggt gggcctgaat
     1261 attatttttg gcgtgctgaa tgtcattaat ttcgcacacg gcgcgcttta catggtggga
     1321 gcctatctgg ctttcacgtg catgaatctt atgctcatgc cgttctggct cactttgctg
     1381 atcgcgccgt tgggtgtggc cattttggca gtgatcgtgg atcgggtcat gttgcgattt
     1441 atctatcagc gcgacatttc cgacagcttg ttgctgacct tcgcgttatt gctcatcttg
     1501 aacgatgcgg tgcgcctgat ctggggaacg ggcatacatg tggtggagcc gcctgtcttg
     1561 ttgcgaggca ccgctcattt gccaggcggt ttggcgtacc ccgtttatag cggctttgtc
     1621 gtggtcattg gattgtgtat tttgtgcgcc ctttggctgc ttttcaatcg gacgcgaatc
     1681 ggaaaaataa tgcgcgccgc cgcgctggac agggaaatgt cccaggcact gtgcattaac
     1741 accaagcttg tgatcaccag tgtatttgcg ttcggcgcat ggctggcggg agtgggtggc
     1801 gttattgccg caccgatgcg cgccatagac cctggcatgg gggacaagat cattattgaa
     1861 agctttatcg tcgtggtgat cggcggcctt ggcagttttc cgggcgcatt gctgggggcc
     1921 attatcctgg gcctgatcta cggctttggc gggcgctatc tgcccgaagt gaatttgatg
     1981 ttgcccttcg tgggcatggc tatcgtcttg cttttaaagc cgcgcggact aatgggcaag
     2041 agggcggcat catgaaaaaa cctttgctga gggtcgtgtt ggcggctgtt ttgctgggct
     2101 tgctt
//
LOCUS       DATCXU010000338         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_491812,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000338 DATCXU010000000
VERSION     DATCXU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491812"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIR04_07650"
     CDS             <1..588
                     /locus_tag="VIR04_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY9460657.1"
                     /translation="PLMFPSTNAGALDGIRRFSDVFGRPVIVYIKNTTFLQPESVEAL
                     DREGRVAAIKYAVVREHPEQDDFLTRLLQCVDKDKVISGIGERPAIVHLRDFGLSGFT
                     SGSVCVGPRGSMALLRAIQSGDFVKAEKLRAAYIPLEDCRDALNPIRVLHDAVTLAGI
                     ADMGPMLPMLSRLDPKDYPQLSAASKALLAHDKAL"
     gene            complement(612..1028)
                     /locus_tag="VIR04_07655"
     CDS             complement(612..1028)
                     /locus_tag="VIR04_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY9460658.1"
                     /translation="MDTKEVDDVQVGASSQITKTISEADVYMYAGITGDFSPNHVDDV
                     YMKAGRYGQRIAHGTLLMGFMSAASAKLYLGRTVSLGYDHVRFISPVFFGDTITTSYT
                     ISKVDYDKKRVHADVRCTNQHGQVVGVALNIRAFVG"
     gene            complement(1090..1974)
                     /locus_tag="VIR04_07660"
     CDS             complement(1090..1974)
                     /locus_tag="VIR04_07660"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9460659.1"
                     /translation="MKISFVGLGQMGKPMAMNMLKSGDDLTVSSRTGAHFAEFEKKGA
                     QTSQDPARIAQSQIIFLCLPDGEVVRDVLFGDHGFASHLKAGQTIVDFSTISYLLTVE
                     IAKRLEGLGVGFLDAPISGMEARAIDGTLTIMCGGKPQTLDKVRPHLECVGSKIIHMG
                     DSGSGQLTKLFNQMLYTINMAGVAEILPLVVKMGLDAERFGQVINSGTGRSHASEFFI
                     PRLLRNHFSDGYSLKKGYKDLTSASEISADLCVPLPTMHAAITTYQMAISKGYGDQDK
                     GIMVRVFEDLLGVHCGKS"
     gene            complement(1990..3084)
                     /locus_tag="VIR04_07665"
     CDS             complement(1990..3084)
                     /locus_tag="VIR04_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460660.1"
                     /translation="MTFLEVRGLVAGYGDKVAVDGISFSVGAGEHLSLLGPSGCGKST
                     TLRCIAGLETPFEGEIVIDSKVVFSSTKKINIPPEKRQLSMVFQSYAIWPHMTVFENV
                     AYGLRAQKVGGAELKQRVGAALSMVGMAELVDRSATDLSGGQQQRIALARSFAVHPKA
                     MLLDEPLSNLDARLRVRMREELKELQHQLKLCTIYVTHDQEEAMAISDRIIVMHGGRI
                     EQIGAPLEIYDLPCTRFVADFIGAANILTGQVAPGAAGQDAVLRVGQAEVLCAPREQM
                     PRPATNGDYLAAVRAVYPELSRGDASGPNQWMATVKRRLLLGDIVTYILDWPGGTLRV
                     QGFPGKLFNEGEQIVLHIPRERAVLVDADR"
     gene            complement(3081..4835)
                     /locus_tag="VIR04_07670"
     CDS             complement(3081..4835)
                     /locus_tag="VIR04_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY9460661.1"
                     /translation="MEQDNRLLSATVGEGGLPQSRRARRPRIKLNLVGLMVLLLLVQG
                     YPILLIVSSAFNVGDPQAIPATEFGWSNFAQILDHMDWVRNSLYVAVGGTLLATAIGV
                     LLAWIIHRTTVPGSGLLEILIAIPYPLGPLVGALSWSALGSPSSGLINKFFTAVTGFV
                     SPIIDINSMGGIIFVMAIFEAPVAVLMIGAAMQRMDPSLEECSSVFGAGRMRTALRVT
                     LPLMLPAILSSALFLFTSMLGAFAIPAILGVASRFYVITTAIYVLFQGYPPDYPMAAA
                     LGLLLIAITAFAVWLYARVLSGRSFTVVSGKNYRPRRVDMGAWTPVLFAIACLYILIA
                     LVLPLGVLLIASLQSGGEIRWDITQWTLKNYHYILVDFPTTRQAIVNSLLLGVGTGTI
                     GTALAAIIAWAVHRYHGPDVRALEQVTMLPQAFPRLIFAFGFLWMLLSLPFPLYGTAL
                     SVLIVYVIVFLPLAYRSMAGVVVQIDRSMEEAAQMSGAAWWRMMRTVTLPLLRSGLLA
                     TWVLIFMVSIREVSASLFLAGPGIQVLGPAIFSFWDSGGLSRVSALAVVQAVIILIAL
                     VLVRWLTSQRGKTAGAQV"
     gene            complement(4811..>5293)
                     /locus_tag="VIR04_07675"
     CDS             complement(4811..>5293)
                     /locus_tag="VIR04_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9460662.1"
                     /translation="AWWYLLKDILPKDYWDRLAAQKPRAYSSSTQIVTDMHNGNLKIG
                     GKVSSFQYVKSKRLKQPIEVVFPPEGAPAQSQVVGIIGPTKRPNAAKAYVNFLLSQEG
                     QQSWNEIQGSPSARTDVDYPGQPDMSHVKIIVPTNMDDFGNTTNHATFVKLWNKITGF
                     "
BASE COUNT         1213 a         1552 c         1472 g         1056 t
ORIGIN      
        1 ccgttgatgt ttcccagcac caacgcaggg gccctggacg gcatacggcg tttctcggac
       61 gtgttcgggc ggccggtcat cgtttatatc aagaacacca ctttcctgca gccggaatcc
      121 gtcgaagcgc tggaccgcga aggccgcgtg gccgccatca aatatgcggt cgtgcgcgag
      181 caccccgaac aagacgactt cttaacgcgc ttgctgcaat gcgtggataa agacaaagtg
      241 attagcggca ttggcgaacg ccccgcaatc gtgcacttgc gcgatttcgg gctgtctggc
      301 ttcacctcgg gttcggtgtg cgttggaccg cgcgggtcga tggcattgct gcgggccatc
      361 cagagcggcg actttgtcaa agccgaaaaa ctgcgggcgg cttacattcc actggaagac
      421 tgccgcgatg cgcttaaccc gatccgcgtg ctgcacgacg ccgtcacgct tgccggcatt
      481 gccgatatgg gcccgatgct gcccatgctc tcacgtctcg acccgaagga ctatccgcaa
      541 ttaagcgcgg ccagcaaagc attgctggcg cacgataaag cgctgtaggc caggctcggc
      601 gctactttcc cttagccgac gaaggcgcga atattaagcg ccacgccgac cacctgcccg
      661 tgctggttcg tgcaacggac atcggcgtga acgcgcttct tgtcgtaatc gactttcgag
      721 atcgtatagc tggtcgtgat ggtgtcgcca aaaaaaaccg gactgatgaa gcgcacgtga
      781 tcatatccca gcgatacggt gcgacccagg taaagcttgg ctgacgcggc cgacataaac
      841 cccatcaaca aggttccatg ggcaatgcgc tgaccgtagc gacccgcttt catgtatacg
      901 tcgtcgacgt gattggggga aaagtcacct gtgatgccag cgtacatata gacgtcggcc
      961 tcggagatgg ttttagtgat ttgcgacgac gcgcctacct ggacgtcatc gacttccttc
     1021 gtatccatgc ttttatcctg ctgttgaaaa tgccgcggtg gcccaaacaa gtggcccaga
     1081 cctgcagcgt tacgacttgc cgcaatgaac gcccagcaag tcttcgaaaa cccgaaccat
     1141 tattccctta tcctgatcgc cgtaaccctt gcttatggcc atttgatagg tcgtgatggc
     1201 cgcgtgcatc gttggcagcg gcacgcacag atcggcactg atttcagagg cgcttgttaa
     1261 atctttgtag ccttttttca atgaataacc atctgaaaaa tgattgcgca aaagccgggg
     1321 aataaaaaat tcagaagcgt ggcttctgcc tgttccgcta ttgatgacct gcccgaatct
     1381 ctcggcatca aggcccatct tgacaaccag gggaaggatt tccgccacac ccgccatatt
     1441 gatcgtatac agcatttgat taaacagctt ggtgagctgg ccgcttccgc tgtcgcccat
     1501 gtgaatgatc ttggaaccca cgcattcaag atggggcctg accttgtcca aagtttgcgg
     1561 cttgccgccg cacatgatcg taagagtgcc atcgatcgcc ctggcctcca ttccggaaat
     1621 gggcgcgtca agaaaaccaa cgcccagacc ctccagcctc ttggcgattt ccacggtcaa
     1681 aagataagag atcgtgctga agtcgacgat cgtttgcccc gccttcaaat ggctggcaaa
     1741 accgtgatcg ccaaacagca catcccgcac gacttcaccg tcgggaagac agaggaaaat
     1801 gatctggctc tgtgcgatgc gcgcaggatc ttgcgaggtc tgcgccccct ttttttcgaa
     1861 ctcggcgaaa tgagcgcccg tacgcgaact gacggtaaga tcatcgccgc tcttcaacat
     1921 attcatggcc atgggtttgc ccatctggcc caatccgacg aagctgattt tcatgttggc
     1981 ggcgcgcctt tatctgtcgg catcgaccag cacggcccgc tcgcgcggaa tatgcaagac
     2041 gatttgttcg ccttcattga ataacttgcc gggaaaaccc tggacgcgca acgttccgcc
     2101 cggccaatcc agaatatacg taacgatgtc gcccaataac agacggcgtt tgacggtagc
     2161 catccactgg ttgggcccgg atgcatcgcc cctgctcagt tccggataga ccgcgcgcac
     2221 ggcggccagg tagtcgccat tggtggccgg tcgcggcatt tgttcccggg gtgcgcacag
     2281 aacctcggcc tggcctaccc gcaataccgc atcctggccg gccgcgccgg gcgcaacctg
     2341 gccggttaat atgttggccg cgccgatgaa gtccgcaaca aaccgggtac acggcaaatc
     2401 atagatctcg agcggtgcgc cgatctgttc aatccgcccg ccgtgcatga caatgatgcg
     2461 gtcggaaatg gccattgcct cttcctgatc gtgcgtgacg tagatggtgc aaagtttaag
     2521 ctggtgctgc agctctttga gctcttcgcg cattcgcacc cgcagacgcg cgtcaagatt
     2581 ggaaagaggt tcgtcgagca gcattgcttt cggatgaacc gcaaagctgc gcgccagggc
     2641 gatgcgctgt tgctgaccgc cgcttagatc cgttgcggac cggtcgacca attcggccat
     2701 gccgaccatc gacagcgcag caccaacccg ttgcttgagt tcggcgccgc caactttttg
     2761 tgcgcgcagg ccatacgcga cattttcgaa cacggtcatg tgcggccaaa tggcgtatga
     2821 ctggaacacc atggaaagct ggcgcttttc aggtggaata ttaattttct tcgtggacga
     2881 aaaaacgacc tttgaatcga tgacgatctc gccttcgaat ggagtctcga gcccagcgat
     2941 gcaacgcagg gtcgtagact tgccgcaacc cgacggaccc agcagggaga gatgttcgcc
     3001 cgcccccacc gaaaaagaga ttccgtcgac cgcaacttta tcgccatagc ctgcgactag
     3061 gccgcggact tcaagaaatg tcatacctgt gctcccgctg tttttccccg ttgactcgtt
     3121 agccagcgca ccagcaccag cgcaatcagg atgattaccg cctggactac ggccagcgcg
     3181 ctgacccgcg acagaccgcc ggaatcccag aaactgaaga ttgccggacc aagaacttgt
     3241 atgcccggac ccgccagaaa tagtgaggcg ctgacttcgc gtatggacac catgaaaata
     3301 agcacccagg tggcgagcaa gcccgatcgc aacagcggca gcgtgacggt gcgcatcatg
     3361 cgccaccagg cggcgccgct catttgcgca gcttcttcca tggaccggtc gatctgcacc
     3421 accacgccgg ccatgctgcg ataagcaagc ggcaggaaca caatcacgta gacgatcagc
     3481 acggaaagcg ccgtcccgta caaggggaag ggcaggctta gcaacatcca caaaaatccg
     3541 aaggcgaata tcagccgcgg aaacgcctgc ggcaacatcg tcacttgctc gagagcgcgc
     3601 acatcgggac catggtagcg atgcacggcc caggcaatga ttgccgccag cgccgtgccg
     3661 atagttccgg ttccgacacc cagcaaaagg ctgttgacga tggcttgccg ggtggtgggg
     3721 aagtcgacca gaatgtaatg gtagtttttc aacgtccact gggttatatc ccagcgtatt
     3781 tcgccgccac tttgaaggga cgcaataagc agcacgccta gcggcaaaac cagggcaata
     3841 agaatataca ggcaggcgat ggcaaacagc accggcgtcc acgcacccat gtcgacacgg
     3901 cgcggacggt aattcttgcc cgatacgacc gtgaacgaac ggccgcttaa aacacgggca
     3961 taaagccaga cggcgaaagc cgtaatggca ataagaagaa gacccagcgc ggcggccatc
     4021 gggtagtcgg gagggtagcc ctgaaaaagc acataaattg ccgtcgtgat gacgtaaaac
     4081 cggcttgcaa cgcccagaat ggccggaatc gcaaacgcac ccagcatcga tgtaaacagg
     4141 aacagcgccg aacttagaat ggccggcagc ataagcggca gcgttacccg caaggcggtt
     4201 cgcatgcgtc ccgcgccaaa cacggacgag cattcttcaa gcgagggatc catacgctgc
     4261 attgcggcgc cgatcatgag gaccgcgacg ggcgcttcga agattgccat cacaaagatg
     4321 atgccgccca tgctgtttat gtctatgatc gggctgacga accctgttac cgcagtgaaa
     4381 aatttattga tcaggcccga actgggcgag cccagcgcgc tccacgacag ggcacccacc
     4441 agaggtccaa gagggtaagg aatggcgatc agaatttcga gcaggccgct gccgggcact
     4501 gtggtgcgat ggatgatcca ggccagtaaa acgccaatgg ccgtcgcgag caacgtgccg
     4561 cccacggcca catagagcga gttccgaacc caatccatgt gatccagaat ctgggcaaag
     4621 ttcgaccagc cgaactctgt cgccggaatg gcctgcggat cgccaacgtt aaacgcgctg
     4681 gagacgatga gcaatattgg atagccctgc acaagcagga gcaacaccat taatcccacc
     4741 aggttcagct tgatgcgggg ccgccgcgca cgccgtgact gcggcagccc cccttcgccg
     4801 actgtggcgc ttaaaagcct gttatcttgt tccacaattt cacaaacgtt gcatggttag
     4861 tggtattgcc gaaatcatcc atattcgtcg ggacgattat cttgacatgg gacatgtccg
     4921 gctgccctgg gtaatcaaca tcagtgcgcg ccgaaggcga accttgaatt tcgttccagc
     4981 tttgctgacc ttcctgcgaa agcaggaaat tcacgtacgc cttggcggca ttgggccgtt
     5041 tggtcggtcc gataatgccg accacctgac tttgcgccgg cgccccttcg ggcgggaaca
     5101 cgacttcgat gggttgcttg aggcgtttgg acttgacata ttggaacgaa ctgaccttgc
     5161 cgccgatctt cagattgccg ttatgcatat cggtgacgat ttgcgtagac gatgaatagg
     5221 cgcgcggctt ctgcgcggca agacgatccc agtagtcttt gggaagtatg tctttaagca
     5281 gataccacca tgc
//
LOCUS       DATCXU010000339         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_282515,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000339 DATCXU010000000
VERSION     DATCXU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282515"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VIR04_07680"
     CDS             <1..202
                     /locus_tag="VIR04_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9460663.1"
                     /translation="VARDSGWITMLPEVVVQDELHSGALVHVGQSSQLTEHFYAITVQ
                     HSHPAGVLNELLLRAPAGDVFT"
     gene            complement(205..1461)
                     /gene="icd"
                     /locus_tag="VIR04_07685"
     CDS             complement(205..1461)
                     /gene="icd"
                     /locus_tag="VIR04_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY9460664.1"
                     /translation="MSYQHIKVPAAGQKITVNADFSLNVPDEPVLPYIEGDGIGADIT
                     PVMLKVVDAAVAKAYGGKRKIHWMEVYAGEKSTRIYGSDVWLPQETLDAVKDYVVSIK
                     GPLTTPIGGGMRSLNVALRQKLDLYVCLRPIRYFKGVPSPVREPEKTDMVIFRENSED
                     IYAGIEFAAGTDQAKALIAFLQDKLGVTKIRFPLTSGIGVKPVSSEGTQRLVRKAFQY
                     AIDNDRPSVTLVHKGNIMKFTEGAFRDWGYELARKEFGATVIDGGPWCKFKNPKTGRE
                     IIVKDVIADAFLQQILLRPAEYDVIATLNLNGDYVSDALAAQVGGIGIAPGANMSDSV
                     AMFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWREAADLIISSMEKSILSKKVT
                     YDFARLLEGATQVSCSGFGQVMIGNM"
     gene            1696..2124
                     /locus_tag="VIR04_07690"
     CDS             1696..2124
                     /locus_tag="VIR04_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY9460665.1"
                     /translation="MFNPSREQVRQFFTDAWSRHKTGGILTPLEIMAVDWVVQHPEYH
                     ADLENPNAADADYPVEQGRTNPFLHLSMHLAISEQLSIDQPPGIKAAHQQLLATRDTH
                     QAAHEIMECLGQIVWESQRLGTVFSNEAYLDLIWKRATHT"
     gene            complement(2184..2546)
                     /locus_tag="VIR04_07695"
     CDS             complement(2184..2546)
                     /locus_tag="VIR04_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY9460666.1"
                     /translation="MKKLILALSGSMLMVVGSLAQAADLAAGQAAFSKFTCSACHGAD
                     AKTTTSPAYPILAGQHEDYLLHALTAYKRGQSGAPASANIRKNAIMGAMAASLSDADM
                     SNIAAWLSSLPTPLTIQK"
     gene            complement(2558..2908)
                     /locus_tag="VIR04_07700"
     CDS             complement(2558..2908)
                     /locus_tag="VIR04_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9460667.1"
                     /translation="MKLRTSLTRTLPALAALAFCAYAGQAQAADAAGSAKAGLDKNSM
                     CIGCHGIPGYKASFPQVYHVPMIAGQNADYIVAALKEYASGARTFPTMEAIAKDLSSQ
                     DMADLAAYYSSFKK"
     gene            3098..>3851
                     /locus_tag="VIR04_07705"
     CDS             3098..>3851
                     /locus_tag="VIR04_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY9460668.1"
                     /translation="MTPAIPAPSRERFQGTETYVATDDLKMAVNAALNLQRPLLIKGE
                     PGTGKTMLAEEVASALGRPLFQWHIKSTTKAHQGLYEYDAVSRLRDSQLGDEKVRHIG
                     NYIIKGVLWRAFEANEPSVVLIDEIDKADIEFPNDLLRELDRMEFYVYETQQTIRARH
                     RPLIIITSNNEKDLPDAFLRRCFFHYISFPDHDTMRRIVAVHYPTIKQDILGAALETF
                     FALRDAPGLKKKPSTSEFLDWLLLLVNDGVSAQ"
BASE COUNT          918 a         1056 c         1054 g          823 t
ORIGIN      
        1 ggtggcgcgc gacagcggct ggatcacaat gctgccggaa gttgtggtgc aggatgaact
       61 gcactcgggg gcattggtgc acgttggcca atcgtcacag ctcactgagc acttttatgc
      121 catcactgtt caacacagcc atccggcggg tgtgctcaac gaactgctat tgcgcgcacc
      181 ggccggagac gtgtttacct gatattacat gtttccgatc atgacctggc cgaagcccga
      241 gcaggaaacc tgtgtggcgc cttcaagcaa acgagcgaag tcgtatgtga cttttttgga
      301 cagaatcgat ttttccatgc ttgaaataat caggtcggcg gcttctctcc agcccatgtg
      361 gcgcaacatc atttcagccg acagaatttc ggaacccggg ttcacgtaat cctttccagc
      421 gtacttggga gcagtgccgt gtgtggcttc gaacatggcg acagaatccg acatgttggc
      481 gcccggggcg atcccgatac cacccacttg ggctgccagc gcgtcggaca cataatcgcc
      541 attcaaatta agcgtggcaa tcacgtcgta ttcggcgggg cgcaacaaga tttgctgcaa
      601 gaatgcgtcg gcgattacat ctttgacgat aatctcgcga ccggtcttgg ggttcttgaa
      661 cttgcaccat ggaccgccgt cgatcacggt tgcgccaaat tcttttcgcg ccagctcgta
      721 gccccagtcg cggaaagcgc cttcagtgaa tttcatgata ttgcctttat gcacaagcgt
      781 gaccgacggg cggtcattgt cgatggcata ctgaaaggct ttacggacaa ggcgctgcgt
      841 gccttcactg gaaacgggct tgacaccgat acccgaggtc agagggaaac gaattttcgt
      901 gacgcccagt ttatcttgca gaaaagcgat caaggctttg gcctgatccg tgcccgcggc
      961 aaattcgatg ccggcgtaaa tgtcttcgga attttcacgg aaaatgacca tatccgtttt
     1021 ttccggttca cgcacaggcg aaggaacgcc cttgaaatag cgtatcgggc gcaagcaaac
     1081 atacaggtca agcttttgac gcaaagcaac gttaagcgaa cgcatgccgc cgccgatcgg
     1141 ggtcgtaagg ggccccttga tggagactac atagtcttta acagcatcga gcgtttcttg
     1201 aggaagccaa acgtcggagc cataaatcct ggtggatttt tcgcccgcgt acacttccat
     1261 ccaatgaatc ttgcgtttgc cgccataagc tttggccacc gcggcgtcaa ccaccttaag
     1321 catgacaggc gtgatatcgg cgccgatgcc atcgccttct atgtagggga gaacgggctc
     1381 atcgggaacg ttgagcgaaa aatcggcatt gactgtaatt ttttgtcccg cggcggggac
     1441 cttgatgtgc tgatatgaca tggatgctcc agttgctccg gaaggaggga attcggtata
     1501 taccaaacga agccgggcat gctgtctggc ccggccatct gcctgaaccg cattatagag
     1561 cacagtctta tataagagta actcaacgcg cttcaaagca accatactgc tgcatgacga
     1621 ccgggcggca agcgaaaacg caccgggata cgcgggtaga atgaatcatt attcaattcg
     1681 aagcgctgta atcttatgtt caacccttcg cgcgagcaag tccgccagtt tttcaccgat
     1741 gcctggagca gacacaaaac gggtggcata ttgactccgc tggaaatcat ggccgtcgat
     1801 tgggtcgtgc aacatcctga atatcacgcg gacttggaga accccaatgc tgccgacgcg
     1861 gactaccccg tggaacaagg acgaaccaac ccattcttgc acctgtccat gcatctggcc
     1921 atttccgagc aactatcgat cgatcagccg cccggcatca aagccgcgca ccagcaattg
     1981 cttgccacgc gcgataccca tcaggccgca cacgaaatta tggaatgttt gggacagatc
     2041 gtgtgggagt cgcagcggct gggaactgtg ttcagcaacg aggcctatct ggatctgatc
     2101 tggaagcgcg ccacgcacac atagacgcgg ccggcctgac actccgcgcc cggaaaacga
     2161 gagcggcgtg tcaggctggt gttttatttt tgaatcgtca gtggcgttgg caagctggac
     2221 agccaggccg caatgttgga catgtcggcg tcggacaaag acgcggccat cgcgcccatg
     2281 atcgcattct tgcgtatatt ggccgaagca ggcgcgccgc tttgaccacg cttgtaagcg
     2341 gtaagcgcgt gcagcaggta atcttcgtgc tggcccgcca aaataggata agccggactg
     2401 gttgttgttt tggcatcggc gccatgacag gccgaacaag tgaatttgga aaaagcggcc
     2461 tggcccgccg ccagatcggc ggcttgggct agcgagccaa cgaccatcag catagagccc
     2521 gacagggcga gaatcagttt tttcattcga gccccgacta tttcttaaaa ctagaataat
     2581 atgccgcaag atcagccatg tcttgagacg acaggtcttt ggcaatggct tccatcgttg
     2641 ggaacgtgcg cgccccggaa gcgtattctt tgagtgctgc gacaatatag tcggcatttt
     2701 ggccggcaat catgggcacg tggtaaacct gaggaaaact ggccttgtag ccgggaatgc
     2761 catggcaccc gatgcacatc gagtttttgt caagacccgc tttcgcactg ccggcggcgt
     2821 cggccgcctg ggcctgaccc gcatacgcac agaaagccaa agcggccagc gcaggcaaag
     2881 tgcgcgtcaa agatgttcgc aacttcattg atagctctta tgtagcggta aataaaagtc
     2941 gatcaatagg cataccgatc aacaaaccgt tcagattgta cgataattgt agcaagccca
     3001 cacggcgcat cggcctatag gcccacagtt cgcatggttc gagcaattaa acgcaatgga
     3061 acgcgccgat gccgcaggac acaaccggac acagacgatg actccagcca ttcccgcccc
     3121 atcccgcgaa cgttttcaag gaaccgagac ctacgtagcc accgacgact tgaagatggc
     3181 cgttaatgcg gcactgaatt tgcagcgccc gttgctgatc aagggcgagc ccggcacagg
     3241 caagacgatg ctggccgaag aagtggccag cgcgctggga cggccgttat tccagtggca
     3301 cattaaatcc accaccaaag cacatcaggg cttgtatgaa tatgacgcgg tgtcccgatt
     3361 gcgcgactcg cagttgggcg acgagaaagt acgccacatc ggcaactaca ttataaaagg
     3421 tgtgctgtgg cgcgcgttcg aggccaacga gccatccgtc gtgcttatcg acgagatcga
     3481 caaggctgac atcgagtttc ccaacgattt gctgcgcgag cttgaccgga tggaattcta
     3541 tgtctatgag acacaacaaa ccatacgggc ccggcatcgg ccattgatca tcattacgtc
     3601 aaacaacgag aaagatctgc cggatgcgtt cctgcgccgc tgcttttttc attacatcag
     3661 tttccccgac cacgacacga tgcggcgcat tgtggctgtt cactacccca caatcaaaca
     3721 agacattctc ggcgcggcct tggagacgtt ttttgcattg cgcgacgcgc caggactaaa
     3781 aaagaaacct tcgacgtcgg aatttctgga ctggctgctg ctactggtga atgacggcgt
     3841 ctccgcccag g
//
LOCUS       DATCXU010000340         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_154717,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000340 DATCXU010000000
VERSION     DATCXU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154717"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="atpH"
                     /locus_tag="VIR04_07710"
     CDS             <1..417
                     /gene="atpH"
                     /locus_tag="VIR04_07710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY9460669.1"
                     /translation="QDDVRAALSDPRINNAQRIELFTGLVKTPLSASAKRFIELLVNN
                     NRLLALPQIAEQFEALKNHREGTALAEITSAFELTDKQVQDLVVGLEKKFGLKLKPAV
                     TVDAKLIGGVRVVVGDQVLDTSVQAQLARMRDTLAA"
     gene            455..1996
                     /gene="atpA"
                     /locus_tag="VIR04_07715"
     CDS             455..1996
                     /gene="atpA"
                     /locus_tag="VIR04_07715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY9460670.1"
                     /translation="MQLNPSEISELLKSRIEGLGASADVRTQGTVVSVTDGITRIHGL
                     SDVMQGEMLEFPNNVFGLALNLERDSVGAVILGDYTGVSEGDPVKTTGRILEVPVGPE
                     LRGRVVDALGMPIDGKGPINAKLTDIIEKVAPGVIDRQSVSQPLQTGLKAIDSMVPIG
                     RGQRELIIGDRQTGKTAVAIDTIISQKGKDVTCVYVAIGQKASTVSNVVRKLEEFGAL
                     EYTIVVVAAASESAAMQYLSAYAGCTMGEYFRDRGEDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNADYVEKFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGVRPAINAGISVSRVGGAAQTKIIKKLAGG
                     IRTDLAQYRELAAFAQFASDLDDATRRQLERGKRVVELLKQPQYQPLQTWELAIALFA
                     VDKGYIDDIAVDHVLAFEKALKDDLKTKKPELVQRIEDTKLLSKEDEAELATAVQDFK
                     KRGAF"
     gene            2035..2946
                     /gene="atpG"
                     /locus_tag="VIR04_07720"
     CDS             2035..2946
                     /gene="atpG"
                     /locus_tag="VIR04_07720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY9460671.1"
                     /translation="MAGIKEIRTKIKSVHNTRKITKAMEMVAASKMRKAQERMRAGRP
                     YATKVRQIAAHLMKALPEYRHPYMMDHGALRAVGVVVVTTDKGLCGGLNTNIMRLVLA
                     RFKEFDQQGVKVQVTALGNKGAGLLTRIGADLVSQEVQLGDTPNLARLIGAIKVQVDD
                     FVNGKIDAVYIATNRFINTMSQDPSFIRLLPLPGGLADPFQSENALVQDQDGAVESTY
                     GWDYLYEPDAQTVIDDLLLRYVEGLVYQAVAENMASEQSARMVAMKAASDNAKKVIGE
                     LELVYNKTRQAAITKEISEIVGGAAAV"
     gene            2996..4414
                     /gene="atpD"
                     /locus_tag="VIR04_07725"
     CDS             2996..4414
                     /gene="atpD"
                     /locus_tag="VIR04_07725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY9460672.1"
                     /translation="MSNGTIVQCIGAVVDIQFPRDSIPNIYDALRLVDESSEYAEHGL
                     TFEVQQQLGDGVVRTIAMGSSDGLRRGMDVSNTDAPISVPVGQGTLGRIMDVLGRPID
                     QRGPIESDERRSIHQHAPKFDELSPSVELLETGIKVIDLVCPFAKGGKVGLFGGAGVG
                     KTVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMIEAEVVKLDNLTESKVAMV
                     FGQMNEPPGNRLRVALSGLTMAEKFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGKLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFQHLDSTVVL
                     SRDIAALGIYPAVDPLDSTSRQLDPQVVGEEHYAVARGVQQTLQRYKELRDIIAILGM
                     DELSPDDKQTVARARKIQRFLSQPFHVAEVFTGSPGKFVPLTETLRGFRMIVDGECDA
                     LPEQAFYMVGSIDEAFEKAKKL"
     gene            4427..>4678
                     /gene="atpC"
                     /locus_tag="VIR04_07730"
     CDS             4427..>4678
                     /gene="atpC"
                     /locus_tag="VIR04_07730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY9460673.1"
                     /translation="MANLHVDVVSAEESIFSGEAKFVVLPGESGELGILPGHTPLISR
                     VRPGTVKIVLADGSGEENIFVAGGILEVQPETVTVLSDTA"
BASE COUNT         1048 a         1265 c         1373 g          992 t
ORIGIN      
        1 caggatgacg tgcgcgcggc gctgtccgac ccgcgcatta acaacgcgca acgcattgag
       61 ctgttcacgg gacttgtcaa aaccccgctt tcggcatcgg cgaaacgttt tatcgaatta
      121 ttggtgaaca ataaccggct cttggccctg cctcaaattg ccgaacaatt cgaggcgcta
      181 aagaatcacc gggaagggac ggcgctggcg gaaatcacta gcgcgttcga gctcaccgac
      241 aagcaggtcc aggatttagt ggtgggcctt gaaaagaaat ttggcctcaa acttaaaccg
      301 gctgtcaccg tagatgccaa attgattggc ggagtgcgtg tggtggttgg cgatcaagta
      361 cttgacactt ctgtgcaggc ccagttggcc cgcatgcgcg atacgctggc cgcttgatgc
      421 gcgccataga taacaggatt ccaggagtct gaacatgcaa ctcaacccgt cagagatcag
      481 cgaactgctc aagagccgaa ttgaaggcct tggcgcgtcg gccgatgttc gcacgcaagg
      541 cacggtggta tccgtgaccg acggcattac ccgcattcac ggtttatctg acgtgatgca
      601 aggcgaaatg cttgaattcc ccaacaatgt gtttggcctg gcgctgaacc ttgaacgcga
      661 ttccgtgggc gccgtgattt tgggcgatta caccggtgtg tcggaaggcg atccggttaa
      721 aactactggc cgcattcttg aagtgccggt gggccccgag ttgcgcggcc gtgtggttga
      781 tgctctgggc atgcctatcg acggcaaggg cccgatcaat gccaagctta ccgacatcat
      841 cgaaaaagtt gcgccaggcg tgatcgatcg ccagtcagtg tcgcagccgt tgcaaacggg
      901 ccttaaagcc attgactcca tggtgcctat tggccgcggt cagcgcgagc tgatcattgg
      961 cgatcgccaa acgggcaaga cagcggtcgc catcgacacg atcatcagcc aaaagggcaa
     1021 agacgttaca tgtgtgtatg tggctatcgg ccaaaaggcc tccacggtta gcaacgttgt
     1081 gcgcaagctc gaagagtttg gcgccctcga atacaccatc gtggtggttg cggcggcgtc
     1141 cgagtcggcg gccatgcagt atttgtcggc gtatgccggc tgcaccatgg gcgagtattt
     1201 ccgcgatcgc ggcgaagacg ccctgatcat ctacgacgat ttgaccaaac aggcatgggc
     1261 ctatcgccag gtctctctgc tattgcgccg gcccccaggc cgcgaagcct atccgggcga
     1321 tgtgttttat ttgcactctc gtctgctcga gcgcgcggcg cgagtgaatg ccgactatgt
     1381 cgaaaagttc accaaaggcg aggtcaaggg caaaacgggc tcgttgaccg ccttgcccat
     1441 tattgaaaca caggccggcg acgtttccgc atttgtgcca accaatgtga tttcaattac
     1501 cgatggtcaa atctttctgg aaaccgattt gttcaacgcc ggggtgcgcc cggccattaa
     1561 cgccggtatt tcagtatcgc gcgttggcgg cgcggcccag accaagatca tcaaaaaact
     1621 ggccggcggc atccgtaccg acttggcaca atatcgtgaa ttggcggcgt tcgctcagtt
     1681 tgcttccgac ctcgacgacg cgacgcgccg tcagctcgag cgtggcaaac gcgtggttga
     1741 attattgaag cagccgcaat accagccctt gcagacctgg gaactggcca ttgccttgtt
     1801 cgcggtcgac aagggctata ttgacgatat cgccgttgat catgtgcttg ctttcgagaa
     1861 agcgcttaaa gacgacttga agacgaaaaa gccagagctg gtgcaacgca tcgaggacac
     1921 caaactattg tcgaaagaag acgaagccga attggccact gccgtacaag atttcaaaaa
     1981 gcgcggtgct ttttaaattc gactggcgca acagcgtcct gacaggaaag tacgatggcc
     2041 ggaattaagg aaattcgaac caagatcaag agcgtgcaca acacgcgcaa gatcaccaaa
     2101 gccatggaga tggtggcggc ttccaaaatg cgcaaagcgc aggaaagaat gcgcgccggc
     2161 cgtccctatg caaccaaggt gcgccagatt gccgcccacc tgatgaaggc gttgcccgaa
     2221 tatcgccacc cttacatgat ggaccatggc gcgttgcgcg ctgtgggtgt cgtggtggtg
     2281 acgacggaca aggggttgtg cggcggcttg aataccaaca tcatgcgttt ggttctggct
     2341 cgcttcaaag agttcgacca gcagggtgtc aaggtgcagg ttaccgcgct gggcaataaa
     2401 ggcgcaggtc ttttaacgcg tattggcgcg gatttggtgt cgcaggaagt gcagttgggc
     2461 gacacgccga acctggcgcg tctgattggc gcgatcaagg tccaggttga cgatttcgtc
     2521 aatggcaaga tcgacgcggt gtacatcgcg accaatcgct tcatcaatac catgagccag
     2581 gatccaagct ttatccgttt gttgcctttg cccggcggcc ttgccgatcc ttttcaaagc
     2641 gaaaacgcct tggtccagga tcaggacggc gcggtcgaat cgacatacgg ctgggattac
     2701 ctgtacgagc ccgatgcaca aactgtcatc gatgatttgc ttttgcgtta cgtcgaaggt
     2761 ttggtgtatc aggctgtggc ggaaaacatg gcctccgagc agtccgcccg gatggtggcc
     2821 atgaaagcgg cgtccgacaa tgccaaaaaa gtcattggcg agcttgagct ggtttataac
     2881 aaaacccgcc aggcggccat taccaaagaa atttcagaaa tcgtgggggg cgctgccgct
     2941 gtttaaggtt taaacagaaa ggcatcccgt caaagatcta tagcaaggac taaacatgag
     3001 caacggtacc atcgttcagt gcatcggcgc cgtggtggat attcagttcc cccgcgacag
     3061 cattccaaat atttacgacg cactaaggct cgtcgacgaa agctcggagt acgccgagca
     3121 cggcctgact ttcgaagtgc agcagcagtt gggcgatggc gtcgtccgca cgattgccat
     3181 gggttccagc gacggcttgc gccgcggcat ggatgtatcc aacaccgacg cgcccatttc
     3241 ggtgccagtg ggtcaaggca cgctgggccg cattatggac gtgctgggac ggccaattga
     3301 tcagcgtggc cccattgaaa gcgatgagcg gcgttcgatc catcagcatg ctcctaaatt
     3361 cgacgagctt tcgccttccg ttgaactgct cgaaaccggc atcaaggtta ttgacctggt
     3421 ttgcccgttt gccaagggcg gcaaggtcgg cctgttcggc ggcgctggtg tgggcaagac
     3481 cgtgaacatg ctggaactca tcaataatat cgccaagcag cacagcggcc tgtcggtatt
     3541 tgccggcgtg ggcgagcgca cgcgcgaggg caacgacttc taccacgaga tgatcgaagc
     3601 ggaggtcgtg aaactcgaca acctcaccga atcgaaagtg gcgatggtgt tcggccagat
     3661 gaatgagcct cccggaaacc gcttgcgcgt tgctctttcg ggcctgacga tggccgagaa
     3721 attccgtgac gaaggacgcg acattctgtt cttcgtcgac aatatttacc gttacacgct
     3781 ggccggaacg gaagtctccg cgctgctggg ccgcatgccg tcggcggtgg gttatcagcc
     3841 cactctggcc gaggaaatgg gcaagttgca agagcgcatc acgtcgacga aaacgggttc
     3901 aattacatcg attcaggctg tttatgtgcc agccgatgac ttgactgacc cgtcgcccgc
     3961 cacgaccttc cagcatttgg actcgactgt tgtgctgtcg cgcgacatcg ccgcgctggg
     4021 tatttatccg gctgtcgatc cgcttgactc caccagccgg cagctcgacc ctcaggttgt
     4081 gggcgaggaa cactacgcag tcgctcgcgg cgtgcagcaa accttgcagc gttataaaga
     4141 gctgcgcgat attattgcca ttctgggcat ggacgagctg tcgccggacg acaagcagac
     4201 tgttgcacgc gcccgtaaaa ttcagcgttt cctgtctcaa ccgtttcacg tggctgaagt
     4261 cttcaccggt tcgcccggca agttcgtgcc attgaccgaa accttgcggg gcttcagaat
     4321 gatcgttgat ggcgaatgcg atgccttgcc cgagcaagcc ttctatatgg tgggatccat
     4381 cgacgaagcg ttcgagaaag ccaagaaact ctaatagggg ctacacatgg caaacttgca
     4441 tgttgacgta gtcagcgccg aagaatcgat tttttccggc gaggcaaaat tcgtggttct
     4501 gccgggcgag tcgggcgaac ttggcatttt gccgggccac acgccgctca tttcacgcgt
     4561 tcggccggga accgtcaaaa ttgtgctggc ggatggcagt ggcgaagaga atattttcgt
     4621 cgccggcggc attctcgaag tgcagccgga aaccgtaacg gtgctttccg atacagcc
//
LOCUS       DATCXU010000341         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_641769,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000341 DATCXU010000000
VERSION     DATCXU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641769"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VIR04_07735"
     CDS             complement(<1..115)
                     /locus_tag="VIR04_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEY9460674.1"
                     /translation="MSALLGCIADDFTGATDLAGMLVQAGMRTVQVVGVPDE"
     gene            complement(112..1086)
                     /gene="denD"
                     /locus_tag="VIR04_07740"
     CDS             complement(112..1086)
                     /gene="denD"
                     /locus_tag="VIR04_07740"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEY9460675.1"
                     /translation="MHIVITGAAGFLGSRLARELLNKGTLTGRTGGPEKITQITLLDV
                     VKPAGFSDPRVRVITGDIADRATIGAALTSDVQGIFHLAAVVSGQAEADFDLGMKVNL
                     DATRQILDSARALATQPRFVFASSVAVFGGDLPAVVPDAMSPVPQSSYGAQKAMCELL
                     VNDFSRKSFIDGRVVRLPTVSVRPGAPNKAASSFASGIIREPLNGQSAVCPVNGDTRM
                     WLMSPRKVVHNLIHAYELDGGQFGASRIVSLPGLSVTVRDMVGALQTVAGADVTRHIQ
                     WLEDETIKRIVTSWPGDFQATRGKALGFKADADFAGIIRAYIEDEHLS"
     gene            1340..>2034
                     /locus_tag="VIR04_07745"
     CDS             1340..>2034
                     /locus_tag="VIR04_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460676.1"
                     /translation="MASVQIRGVQKFFGGTQVIRGVDIDIEDGQFAVLVGPSGCGKST
                     LLRMLAGLEEISQGEIAIDGEVVNNMPPKERDIAMVFQNYALYPHMTVYDNMAFSLTL
                     AKVAKAQIKERVEKASGILGLADLLERYPRQLSGGQRQRVAMGRAIVRNPRVFLFDEP
                     LSNLDANLRVQMRAEIRELHQRLRTTSVYVTHDQIEAMTMADQIVVMRDGVVEQRGRP
                     LDLYDHPANLFVAG"
BASE COUNT          477 a          582 c          551 g          424 t
ORIGIN      
        1 gttcgtctgg cactcccact acctgcaccg tacgcattcc ggcttgcacc agcatgcccg
       61 ccaggtcggt tgcccctgtg aagtcatcgg caatgcaacc gagcagcgcg ctcacgatag
      121 gtgttcgtct tcgatgtagg cgcgaataat gccggcaaaa tccgcatcgg ccttgaaacc
      181 cagcgctttg ccccgcgtcg cctggaagtc gcccggccac gaagtaacaa tacgtttgat
      241 ggtctcgtcc tcgagccact ggatatgccg cgtgacgtca gcgccggcaa cggtttgcaa
      301 tgcgccaacc atatcgcgca cagtcaccga caagcctggc aagctaacga tgcggctggc
      361 gccgaattgc ccgccgtcca gttcataggc atgaatcaaa ttgtgcacca ccttgcgcgg
      421 cgacatgagc cacatgcggg tatcgccatt aaccgggcat accgccgatt gaccattcag
      481 tggctcgcga ataatgccgc tggcaaaact ggaagccgct ttgttgggcg cgccggggcg
      541 cacggaaacc gtgggtaaac gcacgacgcg gccatcaata aatgacttgc ggctgaaatc
      601 gttaacgagc aattcgcaca tagccttctg cgcgccgtac gaactttgcg gcaccgggct
      661 catggcatcg ggaacgaccg ccggcaagtc gccaccgaac acggcaacag aactggcaaa
      721 aacgaaacgc ggctgggtcg ccagcgcgcg ggcgctatcc agaatttgcc gggtagcatc
      781 aaggttgact ttcatgccaa gatcgaaatc agcttcggcc tggccgctga cgaccgccgc
      841 aagatggaat atgccttgaa catcggaggt caatgcagcg ccaatggtgg cgcggtccgc
      901 aatatcgccg gtaatcaccc tgactcgcgg gtcggagaaa ccggctggct tcacgacatc
      961 gagcagtgtg atttgtgtga ttttttcggg gccgcccgtg cgccccgtca gagtcccctt
     1021 atttagcaat tccctggcca gcctgcttcc caaaaagccc gctgcgccgg taatgacaat
     1081 atgcatgcgc ttgctctctt ataaggtgat catacaaatt ttttcgtcaa gtttatgaag
     1141 taatgtcagc gatgtcaata tcgtaaacaa caccggccgg tccatctagg tactatcact
     1201 tagacggccg atttagcctt gaagtgagaa atatctactt gtatgatgat aatagattat
     1261 gcgcgaaaca cccatcatac gtaatgactc aagcgcagca tacctcccga gcataaatag
     1321 acaaaacaag gaacaaggca tggcatcggt gcaaattcgt ggagtacaga aattttttgg
     1381 cggcacccaa gtcattcggg gcgtcgacat cgatatcgaa gacggtcagt tcgcggtatt
     1441 ggtcggcccc tccggttgcg gcaaatcaac cttgctgcgc atgctggcgg gtcttgaaga
     1501 aatcagtcaa ggcgaaatcg cgattgacgg cgaagtggtc aacaacatgc cgcccaaaga
     1561 acgcgacatt gccatggtat tccaaaacta cgccctgtat ccgcacatga cggtctatga
     1621 caacatggcg ttttcgttaa cgctggccaa agtggccaag gctcaaatta aggaacgtgt
     1681 tgaaaaggct tcaggcattc tcgggctggc cgacttgctt gaacggtatc cgcgtcaact
     1741 ctctggcggc cagcgtcagc gggtcgccat gggccgtgca attgtgcgca atccgcgtgt
     1801 ttttttgttc gacgaaccgc tctcaaacct cgatgccaat ttgcgcgtgc aaatgcgcgc
     1861 tgaaattcgc gagctgcacc agcgtctgcg caccacgtcg gtctatgtca ctcacgacca
     1921 aatcgaggcc atgacaatgg ccgaccaaat cgtggttatg cgcgacggcg tggttgaaca
     1981 gcgcgggcgg ccgctggatt tatacgatca ccccgccaat ctgttcgtgg cagg
//
LOCUS       DATCXU010000342         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_481533,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000342 DATCXU010000000
VERSION     DATCXU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481533"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VIR04_07750"
     CDS             complement(<1..528)
                     /locus_tag="VIR04_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY9460677.1"
                     /translation="MRFDKLTTKFQQAIADAQSLAVKHDNPYIEPVHVLAALLADSDS
                     GAGSLLARAGVAVNRVGPALNAAIEALPQVEGAEGNIQVSRELQAVLARTDKEASTRG
                     DTYIASELFLLALAGDKGEAGRILRDAGLQRKALIAAVEAVRGGASVSDAEGEANREA
                     LAKYTTDLTDLARQGK"
     gene            705..935
                     /locus_tag="VIR04_07755"
     CDS             705..935
                     /locus_tag="VIR04_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY9460678.1"
                     /translation="MRGVIKKWGNSAAVRLPSSMMKTLKLDLEDVVDLKIEHGAIVIE
                     PLRTDEYVLGQLLAGIKSKNVHAEIDFGAAGA"
     gene            947..1282
                     /gene="mazF"
                     /locus_tag="VIR04_07760"
     CDS             947..1282
                     /gene="mazF"
                     /locus_tag="VIR04_07760"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEY9460679.1"
                     /translation="MIKRYIPNAGDVVWLHFDRPDATGQEKKTAVVLSPSTYNKKTGL
                     MVCCPITAQIKGYPFEVLIGTADNAAALSDQVKSINWANSKISHRGRVSSVELAQIRA
                     KLHALVFKG"
     gene            1311..2219
                     /locus_tag="VIR04_07765"
     CDS             1311..2219
                     /locus_tag="VIR04_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9460680.1"
                     /translation="MDKFKQLESFVAVATLGSLSAAARAEGVAPAMMGRRIDALEGRL
                     GIKLLARSTRRLSLTSEGSLLLEEAQRILRDLGDTEARIAQGSAKPSGHLRISAPAGF
                     GRRHVAPLLPAFTQTYPDVTLTLDLTDRLVDLIEERYDCAIRIGELDDSQIVGIRLAD
                     NQRVIVAAPSYLHVHGRPNTPEDLTRHNCLSFGAQGNQAKGWLLKQKGQTRAVRVKGN
                     LACSDGAVLHEWALAGYGLAWRSLWEVREDLTTGRLVSVLDEYAAPSNGIYAMLPERK
                     HLPLRVRVFVDLLKTSYAQASYWESH"
     gene            2299..3120
                     /gene="queF"
                     /locus_tag="VIR04_07770"
     CDS             2299..3120
                     /gene="queF"
                     /locus_tag="VIR04_07770"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523221.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEY9460681.1"
                     /translation="MPDPLLAGIPLGHDVAYPKSYDPGLLFPVARQEKRRTLPVHYEW
                     VGADIWNAYEISWLNAHGKPVVGTARFIFPQTSPNLIESKSLKLYLNSFNETHFENSG
                     TARLCMERDLSQAAGGQVDVSIDVGHTQKSASQALDGVCIDDADVVIDIYQRDPNLLR
                     CLPAACTVTESLMSDLLKSNCPVTGQPDWGSVQIRYTGRQIDRAALLKYIVSFRRYDG
                     FHEHCVEQMYSDIWQACRPESLLVYARYTRRGGLDINPWRSSTTAEVSQACTFRQ"
     gene            complement(3153..3734)
                     /gene="pgsA"
                     /locus_tag="VIR04_07775"
     CDS             complement(3153..3734)
                     /gene="pgsA"
                     /locus_tag="VIR04_07775"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY9460682.1"
                     /translation="MPINFPIVLTWLRIAMIPLIVGLFYIPAEWIPGPARDVIAAGAF
                     ILAALTDWLDGWLARRWNQTSAFGAFLDPVADKLIVCAALLVLLDLNRVDSFIALIII
                     GREITISALREWMAKIGASGSVAVHWLGKVKTIAQMVAIPCLLYWQPIYDISTAGVGR
                     VLIIVAAILTVWSMCYYLRRAWPSIRDKADREP"
     gene            complement(3750..>4652)
                     /locus_tag="VIR04_07780"
     CDS             complement(3750..>4652)
                     /locus_tag="VIR04_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY9460683.1"
                     /translation="VMPAVVVCSHALPDPGLIDLLAEQTNGKTRLVTKPRGVRKTWLL
                     QAQKNAEMALARLLSESSARAARTLALASTLELDTDEASLDALHVECFDISHTAGEAT
                     QASCVVYEHHAMQPSLYRRFNIAGITPGDDYAAMRQVLKRRFERVADGQIEMPDIVLI
                     DGGKGQVEVARQVFVELGLDVHAIVGVAKGEGRKVGLETLVFVDGRAPVALGIESAAL
                     MLVAQIRDEAHRFAITGMRARRAKTRNVSRLEEIEGVGAKRRQKLLARFGGFSGVVSA
                     SVDDLRSVDGISEELAERIYQAVR"
BASE COUNT         1071 a         1395 c         1249 g          937 t
ORIGIN      
        1 cttgccctgc cgcgccagat cggttaaatc ggtggtgtat ttcgccagcg cctcgcgatt
       61 ggcctcgcct tcggcgtccg ataccgaggc gccgccgcga accgcctcga ccgctgcgat
      121 gagggccttg cgctgcaggc cggcgtcacg caaaatacgg ccagcctcgc ccttgtcgcc
      181 ggccagggca agaagaaata gctcgcttgc tatataggtg tcgccccggg ttgaagcctc
      241 tttgtcggtg cgagccagta ccgcctgcag ctcccggctg acctggatat tgccttccgc
      301 cccttcaacc tgcggcaatg cctcgatggc ggcgttaagg gcgggtccca cccggttaac
      361 cgccacgcct gcacgcgcga gcaggcttcc ggcgccgctg tcagagtcag ccagcaaggc
      421 agccagcaca tgaaccggct cgatataagg attatcatgc ttaacagcca ggctttgcgc
      481 atcggcgatg gcttgctgga atttagtcgt caatttatca aatcgcataa tcgttttgtc
      541 tgaaagatgg gaaggcgtct gcccgaacat tgattatgtg cggcctgtat gcccaatttc
      601 aagatgcccg gcagcatacg gcgaagtccc aaaagcaaat tgtttataca ctgcataaac
      661 tggtaaccta catttcgtcc tttcgacgcc taaaggagtt gctcatgcgt ggagtaataa
      721 aaaaatgggg caatagcgcc gcggtgcgct tgccttcgtc aatgatgaaa accctcaaac
      781 tcgatctgga agatgtcgtc gatcttaaaa ttgagcatgg agccattgtg atcgaacccc
      841 tgcgcactga cgaatatgtg ctgggccagt tgctggcggg aatcaagtct aaaaacgtgc
      901 acgctgaaat tgactttggc gcggccggcg cctgagcgag gcctccatga tcaaacgtta
      961 tattcccaat gccggcgacg tcgtctggct gcacttcgat cgccccgacg ccacgggaca
     1021 ggaaaaaaaa acggccgtgg ttcttagccc gtcgacctac aataaaaaga cggggctaat
     1081 ggtgtgttgc cccataacgg cgcaaatcaa gggctaccca ttcgaagtgc taattggcac
     1141 tgcggacaac gcggcggctc tatctgatca ggtcaaaagc attaactggg cgaacagcaa
     1201 aatcagccat cggggccgtg tcagttccgt cgaactggcg caaatccggg ccaaactgca
     1261 cgcccttgta ttcaagggat gaaaagcccc cgggcacgaa aacaattgcc atggataagt
     1321 tcaaacaact cgaaagtttt gtggccgtcg cgactctggg cagcctgtcc gccgcggcca
     1381 gggctgaggg agtggcccct gcgatgatgg gccggcgcat tgacgcgctc gagggtcggt
     1441 tgggcatcaa gctgttggcg cgatcaaccc ggcgtttgtc gctgacatcg gaagggagcc
     1501 tgctgctcga agaagcacaa cgcattttgc gtgacttagg cgacaccgaa gcgcggatag
     1561 cgcaaggcag cgcaaaacct agcggccatt tgcgcatcag cgcgcccgcc ggcttcggga
     1621 gacgccacgt ggcgcctcta ttgccggcgt tcacccaaac ttatccagat gtaacgctaa
     1681 cgctcgatct taccgaccgc ctggtcgatc tgatcgagga acgctacgat tgcgccatac
     1741 ggatcggaga actcgatgac tcgcaaatcg tgggaatacg gctggctgac aatcagcgcg
     1801 ttattgtcgc cgccccgtct tatttgcatg tccatgggcg acccaacacg cccgaggatc
     1861 tgacgcgcca taattgtctg tcgttcggcg cccagggcaa ccaggccaag ggctggctgc
     1921 ttaagcaaaa agggcaaacc cgggctgtcc gggtcaaagg aaacctggcg tgcagcgacg
     1981 gagccgtatt gcacgaatgg gcactggcgg gctatggcct ggcgtggcgt tcgctgtggg
     2041 aagtccgcga ggatctgact acgggccgtt tagtcagcgt gctggacgaa tacgctgctc
     2101 catcgaatgg catatacgcc atgctgcccg aacgcaagca tttgcccttg cgcgtgcgag
     2161 tcttcgtcga tctgctaaaa accagctacg cgcaagcctc ttattgggaa tcgcactagc
     2221 aacgcgccga tgtaaaaaca atcggcgcga acggtacaat gtttgtttca ccttgcaccc
     2281 gttttggact ttgccggtat gcctgatccg cttcttgctg gaattccgct gggccatgac
     2341 gtagcctacc caaaatccta cgatccaggc ctgttgttcc ctgtcgcgcg ccaggaaaaa
     2401 cgccggacgc tgcctgttca ctatgaatgg gtcggagccg acatctggaa tgcctacgaa
     2461 atttcgtggc tcaatgcgca cggaaaaccg gttgtcggca cggcgcgttt catatttccg
     2521 cagaccagcc ccaatctgat tgaatcgaaa tcattaaagt tatatttgaa ttcgttcaat
     2581 gaaacacact tcgaaaatag tggaaccgca cgcctgtgca tggagcggga tctaagtcag
     2641 gcggctggcg gccaggttga tgtaagcatc gacgtcgggc acacgcaaaa aagcgcttca
     2701 caggcgctcg acggagtgtg catcgacgac gccgacgtcg ttatcgacat ctaccagcga
     2761 gatcccaatc tgctgcgctg cttgcctgct gcttgcacag taactgaaag cctgatgtcc
     2821 gatctgctta aatcaaactg ccccgtcacc ggtcaaccgg actggggcag cgtgcaaatc
     2881 cgctacaccg gccgccagat cgatcgcgcc gcgctgttga aatatatagt atctttccgt
     2941 cggtacgatg gctttcacga gcattgcgtg gaacaaatgt acagcgacat ctggcaggct
     3001 tgccgccctg aatcactatt ggtctacgcc cgctacaccc gccggggagg gctggatatc
     3061 aacccgtggc gtagcagcac gactgccgaa gtaagccagg cctgcacgtt cagacaatag
     3121 cttgccgtcc gcgccaacag gcattgacga cgttatggtt cccggtctgc cttgtcccga
     3181 atggaaggcc acgcccggcg caagtaatag cacatagacc ataccgttaa aatagccgca
     3241 acaataatca gcacgcgacc cacccccgcc gttgagatat cgtagatcgg ctgccagtac
     3301 aacaggcaag gaatggcgac catttgcgca atcgttttca ccttgcccag ccagtgcaca
     3361 gcaacactgc cgctggcgcc gattttggcc atccattccc gcaatgccga aatcgtgatt
     3421 tcgcgtccaa tgataatcag ggcaataaac gaatcgacac gattcaaatc cagtaatacc
     3481 agcaatgccg cacatacaat cagcttgtcg gccacgggat ccagaaatgc gccaaacgca
     3541 gaagtctgat tccagcgtct ggccagccaa ccgtcgagcc aatcggtaag cgccgccaaa
     3601 ataaaagcgc ccgcggcgat gacatcgcgc gcgggcccgg ggatccattc tgcgggaata
     3661 taaaacaacc ccacaattaa cggaatcatg gcgatgcgca gccaagtcag gacaatcggg
     3721 aaattaatcg gcataaagcg aatattaccc tatcgcaccg cctggtaaat ccgttcggcc
     3781 agctcctcgg aaataccgtc aacggaccgc aagtcatcga cactggcgct gaccactccg
     3841 gaaaagccgc caaagcgagc caacaatttt tgccggcgtt tcgctcctac cccctcgatt
     3901 tcctccagac gcgacacatt gcgtgtcttg gcgcggcgcg cccgcatgcc cgtaattgcg
     3961 aaacgatggg cctcgtcgcg aatttgcgcg accagcatta gcgcggccga ttcgataccc
     4021 agcgccacag gtgcgcggcc gtcaacaaac acgagagtct ccaggcccac cttgcgccct
     4081 tccccttttg caacgcccac aatggcatgc acgtccaggc ccaattcgac aaacacctgc
     4141 cgcgccactt caacctggcc cttgccgccg tcgatcaata cgatgtccgg catttcgatc
     4201 tgaccatccg caacgcgctc gaagcggcgc tttaatactt gccgcatggc ggcgtaatca
     4261 tcacctggcg taatgccggc aatattgaat cggcggtaca acgaaggctg catggcatga
     4321 tgctcataca ccacgcatga tgcttgcgtt gcctcgcccg ccgtgtggct tatgtcaaag
     4381 cattccacgt gcagtgcatc aagcgatgct tcgtcggtat caagctcaag tgtcgaagcc
     4441 agcgcaagcg tgcgggccgc ccgcgcgctt gattccgaca ataaacgagc caatgccatt
     4501 tcagcatttt tctgcgcctg caataaccat gttttgcgca ccccgcgcgg cttggtcacc
     4561 aaccgtgtct tgccatttgt ttgttcggcc agcaaatcga tcaggcccgg atcaggcaac
     4621 gcgtgcgaac acaccaccac agccggcatt ac
//
LOCUS       DATCXU010000343         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_252033,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000343 DATCXU010000000
VERSION     DATCXU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252033"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..750)
                     /locus_tag="VIR04_07785"
     CDS             complement(352..750)
                     /locus_tag="VIR04_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY9460684.1"
                     /translation="MERKTQIIRDANDSGRGPGPDVMASSTLEGTDVYNQAEQKLGTI
                     QDIMIDIERGRVAYAVLSHGGVLGIGEKLFAIPWSALTLDTQRECFMLDIDLQRFKDA
                     EGFDKDNWPRMGDLAWATRVHEYYNQPPYW"
     gene            975..1529
                     /locus_tag="VIR04_07790"
     CDS             975..1529
                     /locus_tag="VIR04_07790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY9460685.1"
                     /translation="MKATLSELPPNLPIPTDDGAAAHLEGLALPQLSLPSTDGTRVDL
                     SKLAGRWVIYIYPLTGQPGVPLPAGWDDIPGARGCTPQSCAFRDHYAELRSLDTNVFG
                     LSAQTTSYQREARDRLHLPFELLGDSPMHLKDILHLPTFNVAGMELFKRLTLIIDDGR
                     ISKVFYPVFPSDQNAAEVVAWLKK"
     gene            1538..>2332
                     /locus_tag="VIR04_07795"
     CDS             1538..>2332
                     /locus_tag="VIR04_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY9460686.1"
                     /translation="MIQSSPLFTPINIAGRESRHRVMMAPMTRARSTQPGDVPNIMNA
                     RYYAQRASAALIVTEATQISPQGKGYSCTPGIYSAAQVAGWRLVTDTVHAAHGRIFLQ
                     LWHVGRMSHTDFHNGQLPVAPSAVPFDAQIWKVNAITGQGEMVNCPTPRALAKTEIRT
                     VIADFRQGARNAIAAGFDGVEIHGANGYLVDQFLRTTANIRHDEYGGSRVNRLRFLKE
                     VVAAVAAEVGAARTAIRLAPFLTARGMACPDILPTILEAAEFLQAPG"
BASE COUNT          494 a          700 c          619 g          519 t
ORIGIN      
        1 ggctaaaacc gccgccgata aggcgcgcaa ggctgccgca gcgacggctc tgtggatgtt
       61 tgtggctttg ttatgcggag ccttttccgc cagttttttt gccatttatg gtggccggcg
      121 ccgcgatgcg gccgttattg aactgagggc aattccgtaa aaaagtccat cacacccaaa
      181 gcttcaaatc attgatggcg ttatgcggcg cctttcatca aagaggaaaa ttaaaatgcg
      241 ttcaatactt ttatggatgc ttggcatccc gatcccgatt atcattttga ttgcattatt
      301 acgctaagac aatgaatcaa tatcaccagc cgcaatgggc gctgtcaaag cttaccagta
      361 cggcggttgg ttatagtatt catggacgcg ggttgcccat gcaaggtcgc ccatgcgcgg
      421 ccaattgtcc ttgtcaaatc cttcggcgtc cttgaaacgc tgcaggtcaa tatcaagcat
      481 gaagcactcg cgttgtgtat ccagtgtcag tgcactccat gggatggcaa atagcttttc
      541 accgatcccc agcaccccgc cgtgagacaa taccgcatag gctacgcgcc cacgctctat
      601 gtcaatcata atatcctgga tcgtgccaag cttttgttcc gcctggttgt agacgtcagt
      661 gccttccagg gtggaagagg ccatcacgtc tggaccgggt ccgcggccag aatcattcgc
      721 gtcgcggata atttgggttt tgcgctccat tgcgttgctc cttgaatgac gctcggaagg
      781 cgaacttgcc ttcgccattg cagcagcata cgttgtgccg cggcaaagtc gtgctgcacg
      841 acttcaacgt gacccgcttg cgcagtgcgt tgctgtccct ataataccca gcggggccat
      901 ccggcgaaca ttacacctgt cgcgccgcga gtgccgatgc aattccccca actcacaaac
      961 ccgaaccggc tattatgaaa gccacgcttt ccgaacttcc gccaaattta cccatcccca
     1021 ccgacgatgg cgccgcggcg catctcgaag gtttagccct tcctcaactt tcactgccca
     1081 gtaccgacgg cacacgtgta gatttaagca agctggccgg acgatgggtt atttatatct
     1141 atccgctgac aggacagccc ggggttcctc tacccgccgg gtgggatgac attcccggcg
     1201 ctcgcggctg cacgccacag agctgcgctt ttcgcgacca ttatgccgaa ctgcgttcac
     1261 tggatacaaa tgtgtttggc ttaagcgcac aaacgaccag ctaccaacgc gaagcccggg
     1321 acagattgca tctgcccttt gagcttcttg gcgacagccc tatgcacctg aaggatatcc
     1381 tgcacttgcc cacattcaac gtcgccggaa tggagctatt caagcggctg acgcttatta
     1441 tcgacgacgg gcgaatcagt aaggtctttt accctgtatt tccttccgac cagaacgcag
     1501 ccgaagtagt ggcgtggctc aaaaaataga gcattgcatg attcagtctt cacccttgtt
     1561 cacccccata aacattgccg gccgcgagtc gcgccaccgt gtgatgatgg ctcctatgac
     1621 ccgcgcccgc agcacacaac ccggagatgt tcccaacatc atgaatgcgc gctactacgc
     1681 gcagcgcgca tcggctgcac tgatcgtgac ggaagccacc cagatatcgc cgcaaggcaa
     1741 ggggtattcc tgcacgcccg gcatttatag cgcagcgcaa gtggccggtt ggcggctggt
     1801 gactgacacc gtacacgctg cgcacggccg catcttcctt cagctttggc atgtgggccg
     1861 aatgtcccat acggattttc ataacggcca acttccggtg gcgccttcgg cggtgccatt
     1921 cgacgctcaa atatggaaag taaacgcgat caccggccaa ggcgaaatgg tgaactgtcc
     1981 cacgccccga gcgcttgcga aaacggaaat tcggaccgtt atcgccgact tccggcaagg
     2041 ggcgcgcaac gccatcgcgg cgggttttga cggcgtggag attcacggcg ccaatggtta
     2101 tctggtggac cagttcctgc gcaccaccgc caacatccgc catgacgagt acggtggatc
     2161 gcgcgtcaat cggctgcgtt ttctcaaaga agtggtggct gcggtggcag cggaagtcgg
     2221 cgcggcgcgc accgccatcc gtcttgctcc attcctgacc gcgcgcggga tggcgtgccc
     2281 ggatattctt cccaccattc tggaggccgc cgaatttctg caggccccag gc
//
LOCUS       DATCXU010000344         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_675183,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000344 DATCXU010000000
VERSION     DATCXU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675183"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1014
                     /locus_tag="VIR04_07800"
     CDS             271..1014
                     /locus_tag="VIR04_07800"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY9460687.1"
                     /translation="MTKAALSLSATVDRPAPRPWLFMASRVAAAGPVAVALIIVALHA
                     IKPEFQPSWRFISEYAIGPHGWIMKLAFLIWAASCAALALTLKHELKSWPGKIGVAVL
                     LIVAVALVPAGLFAQDPVTAKPHELTTSGNIHAIASMIGIPGIPIAAMLISSSLWRTN
                     QAWTPYRRSIMWAAHAAWISLVLMGLYLAWAVPRAGGFNADVWAGWMNRLVVATYIAW
                     QFTIAYRLITISKAQDRTSDLRFRKTACK"
     gene            complement(1042..1737)
                     /locus_tag="VIR04_07805"
     CDS             complement(1042..1737)
                     /locus_tag="VIR04_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY9460688.1"
                     /translation="MRFIALAFSCLLIFSMPANANDVANFNAMVALANKGDAEAQYHV
                     GMMYNNGIGTQKDPRQAFEWFQKSTASNDPLGAYKLGCYYDGQGAGIVATDSTEALKY
                     KLVAAKAGYALAQYDVALIYGRQENFEEALKWSKMAGDQGYPKALYNLSGLYFQGKGA
                     PKDLALAYAYFKLSKVPPRKQVNEMAGILTKPELERAEKLVSEWKPQPTALTLKAMRG
                     VSAAEELLKAAKK"
     gene            complement(2023..2589)
                     /locus_tag="VIR04_07810"
     CDS             complement(2023..2589)
                     /locus_tag="VIR04_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460689.1"
                     /translation="MAATLLTGDFLWVTLLAAFVGVVVGETGFCLKTDRRVGRLAVLG
                     SIIFSITVIAGFVGYKIFYPPEPTPFVYVKRTNATVRTGNMKSALELKYKYIAGCRDP
                     SDTLLWIEIATGGARREHPSTLADFQAQYLHDCGFYTIDTREFVEGSAVTFGGVDMLC
                     IKDLFNTNICHWIPASAIELDPPEFEQR"
     gene            complement(2590..3027)
                     /locus_tag="VIR04_07815"
     CDS             complement(2590..3027)
                     /locus_tag="VIR04_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460690.1"
                     /translation="MGISDWWSDLFEFWWDLFDNPLHSIYAVGFCFVVVLIATVLILW
                     WRPAEVRKLAWRVFLASAGFCAAIFGLWFKPALTSYSWIDLLITSGFGVGLVLVVCIA
                     NYWCVAILLVWRYNNEHRHSWHVWTAWLFTTGWISWSLFNLGL"
     gene            complement(3124..3591)
                     /locus_tag="VIR04_07820"
     CDS             complement(3124..3591)
                     /locus_tag="VIR04_07820"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY9460691.1"
                     /translation="MNLFQLLFGEWIDLLFGFHGRINRAKYWFTFVIYFVALFALYLL
                     FSLFFSFPTDLLGLVLVSSVPLIPITISSVAVAIKRLHDRNKSGWWVVAFYVLPGVIG
                     NIGPYAGLDTVFQLASLALSIWAVIELGLLRGTSGRNPYGPDPLAPKNVRVRG"
BASE COUNT         1004 a         1091 c         1080 g          875 t
ORIGIN      
        1 aacaacatcc tggctgcatt cagcacgcga ctcttggtgg catcggccgc ctcggtccgt
       61 aaatctgatg cgtactcacg tcgttttttg cgtcttgcca tattggatat agtatactat
      121 atttaattgt cgccgatcca agagtctggg accggtgacg tccaggccta tctcgacaac
      181 attgacagga agacgggcaa aactcccgat gatttttgtg cgttggcgga aaagggctcg
      241 tcaggccgcc aaagtgggaa ccagaacaaa atgacgaagg ctgcattgtc tctgtcggcg
      301 acggtcgacc gtccggcccc gcgtccttgg ttgttcatgg cgtctcgtgt tgcagctgcg
      361 ggaccggtgg ccgttgcgct catcattgtc gccctgcatg ccatcaagcc ggaattccag
      421 ccgtcatggc gtttcatcag cgagtatgcg atcggcccgc acggatggat catgaaactc
      481 gccttcctga tctgggcagc aagttgcgcg gcattggcgc tgaccttgaa gcacgagttg
      541 aaaagctggc caggcaagat cggcgtggct gttctgttaa ttgtcgcagt cgcgctcgtg
      601 cctgcgggcc tgttcgcaca ggacccagtg accgcgaagc cccatgaatt gacaacgagc
      661 ggtaacatcc acgccatcgc gtccatgatt ggcataccgg gaattccgat cgccgccatg
      721 ctgatcagct caagcttgtg gcgaactaat caggcatgga cgccctatcg gcgctctatc
      781 atgtgggcgg ctcatgcggc gtggatcagt cttgtgctga tgggactcta tctggcgtgg
      841 gcggtgccgc gggctggggg attcaatgcg gatgtctggg ccggctggat gaaccggctg
      901 gtggtggcca cctatatcgc gtggcaattc acgatcgcct atcgcctcat aacgatcagc
      961 aaggcgcagg atcgaacctc cgaccttcga tttaggaaaa ccgcctgcaa gtagcgcgat
     1021 tttgaagcag gcagagaatc cttatttctt ggcggctttt aagagttctt ctgctgctga
     1081 gacgccgcgc atcgccttaa gggtcaaagc tgttggttgt ggcttccatt cggaaaccag
     1141 tttttctgct ctctcgagtt ccggtttggt cagaataccg gccatctcat tcacttgttt
     1201 tctgggcggg acttttgaaa gtttgaaata cgcatacgct aaagctaaat cttttggcgc
     1261 cccttttcct tgaaaataca atccggacaa attatagagc gctttaggat acccttgatc
     1321 gcccgccatc ttcgaccatt ttaatgcttc ctcgaaattt tcttgtcggc cataaataag
     1381 ggcaacatcg tactgggcaa gcgcataacc ggctttggca gcaacaagtt tgtattttaa
     1441 tgcttcagtc gagtcggtgg ctacaatgcc cgcaccttgc ccgtcataat aacaaccgag
     1501 cttgtaagct cccagcgggt cattagacgc agttgatttt tgaaaccact caaatgcctg
     1561 cctgggatct ttttgtgttc cgatgccatt attatacatc atgccaacat ggtattgcgc
     1621 ctctgcatct cctttattgg ccaatgcgac catcgcgttg aagttggcca cgtcatttgc
     1681 attggcgggc attgaaaaaa ttaaaaggca cgaaaaagct aaggcaataa atctcatttt
     1741 ggctccccga ttgggttcga aagattatat cttacttgag catgccgatg cgcgcacctg
     1801 tccgtgggaa gcggccacgc ggctgccgcg ccaccaaaaa gaatgacaaa ttcgcatcgt
     1861 ctcattgcct ccccgatgct gggacaggac atcgtacagg gtcggccgcg cgaggcagcc
     1921 aatgtccgct tcgggccaaa gcggacattt gcagcgcaaa aggctcaaaa gcggacatgt
     1981 gcggtgcaaa caggaatgtc cgctaagggc caaaggcgaa cctcaccgct gctcgaactc
     2041 aggcgggtct agttcgattg ctgacgccgg aatccaatga cagatgttcg tattgaaaag
     2101 atccttgatg cacagcatgt caacgccgcc gaaggttacc gccgatccct cgacaaattc
     2161 gcgcgtgtcg atggtgtaga acccacagtc gtgaaggtac tgcgcttgaa agtcggccag
     2221 tgtcgacggg tgctcacgtc gcgctccacc tgtggcgatc tcgatccata gaagagtgtc
     2281 agaaggatct ctacagccgg caatatactt atactttaac tcaagtgcag atttcatatt
     2341 tccggtccgg acagtcgcat tcgtacgttt cacgtataca aacggcgttg gctctggagg
     2401 gtagaatatt ttgtatccaa cgaagcctgc gatcactgtt attgaaaata taattgaacc
     2461 tagtacggca aggcggccaa cccggcggtc ggtctttaga caaaaacctg tttccccaac
     2521 aactacgcca acaaaggccg caaggagcgt gacccagaga aaatcaccgg tcagcagagt
     2581 ggcggccatt taaagcccaa ggttgaaaag cgaccaggaa atccagccag tggtgaacaa
     2641 ccaagcggtc cagacatgcc agctgtggcg atgctcattg ttgtaacgcc agaccaaaag
     2701 aatggcgacg caccagtaat tggcaatgca tacaacaaga accagcccaa caccgaaccc
     2761 cgaagtaatc aaaagatcga tccaagaata tgaggttaag gcaggcttga accacagacc
     2821 gaaaattgcc gcgcaaaaac cagccgaagc caaaaagacg cgccaagcga gcttgcgaac
     2881 ctcagcaggg cgccaccata aaattaaaac agtcgcgatc aggacgacta caaagcaaaa
     2941 gccaactgca taaatggagt ggagcgggtt atcaaataaa tcccaccaaa actcaaataa
     3001 gtcggaccac caatcagaaa tgcccattgc ggccccgcat tatcccgagt tttttcacac
     3061 ttcgtatttc attggcaatg tccgctttgg gtcataggcg gacatgtgca cactaggttc
     3121 gccctatccc cgaacacgga catttttcgg cgcaagcggg tctggtccgt acgggttgcg
     3181 tccggacgtc ccgcgcagga ggccgagttc aatcacagcc cagatcgaca gtgcaaggct
     3241 cgccagctga aacaccgtgt ccagcccggc ataggggccg atgttaccaa tcacgcccgg
     3301 cagcacgtag aacgccacca cccaccagcc gcttttgtta cggtcgtgca ggcgcttgat
     3361 ggcgacagcg acactcgaaa tggtgatggg gatcaagggc acagaggaaa ctaagacgag
     3421 gcccaacaaa tcggtaggga aggaaaaaaa gagactgaag aggaggtaca gagcgaacag
     3481 agcgacgaaa tagatgacga aagtaaacca atatttcgcg cggttaatgc gtccgtgaaa
     3541 accgaacaac aggtcaatcc attccccgaa caacaactga aatagattca tggccgcccc
     3601 aagtttgtga caccgtatcg taccggctga aaataggcga tttgaatgac ttctgcgaaa
     3661 ttcaagcaag tcggtaaaat cgctagcgag tgtccgctct gagttcagga gcggacattg
     3721 ccacatgtcc gcgtgacagt tgctttgccc tcagaagcag acatgtgcag tgcaccagca
     3781 tgtccgctaa ggccatcagc ggactcatgc agcgcaacca gacggcctat gggatcacct
     3841 catcggcgcg ggcgagcagt gtcggcggca cggtgagtcc caatgctttt gcggtctgga
     3901 ggttgactac caggaggaat ttcactggcc gcccgatcgg caagtcggcg ggtttcgcgc
     3961 catttagaat cttgtccacg aagcctgccg cgcggccccc ggcagcccgg gtgcgtcggg
     4021 ctacgctccg ggacgttcga ctacgggcgc
//
LOCUS       DATCXU010000345         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_267757,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000345 DATCXU010000000
VERSION     DATCXU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267757"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /gene="lpxK"
                     /locus_tag="VIR04_07825"
     CDS             complement(<1..550)
                     /gene="lpxK"
                     /locus_tag="VIR04_07825"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY9460692.1"
                     /translation="MNAHGFLIRQIQTIWQRKGIASTVLLPLSWIARWAIARKKSQYE
                     CGRRTTAQSSRPVLVIGNLYVGGTGKTPVVIALAQALKARGWQPGVISRGYRVAIGKQ
                     ARAGRGALNPAEFGDEPALIARLAQVPVAVHPQRVLALRELERAYPDVNVIISDDGLQ
                     HRALGRDIELLVQDDRGLGNGRV"
     gene            complement(547..957)
                     /locus_tag="VIR04_07830"
     CDS             complement(547..957)
                     /locus_tag="VIR04_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY9460693.1"
                     /translation="MNFRRRLAADEPEINLIPLIDVLLVILIFLAVTTSFTRLNQLKV
                     ALPQASADAVDTTAINVAVSQDGLYAINGLLLPGRDVDEIAQALRTAADDKKDPVLVI
                     NADAQSTHQAVVRVMQAARMAGISRVNFATESSP"
     gene            complement(954..1562)
                     /locus_tag="VIR04_07835"
     CDS             complement(954..1562)
                     /locus_tag="VIR04_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY9460694.1"
                     /translation="MLQIIDAAGWPIWLLLATSVLGLALIIERFLSLRRKLIMPANLS
                     AQVADLIQRNQQTPEAVDRLAESSPLGRVLAEVLVNSAETNAYRVAAIEDVGKDIGHQ
                     LNRYLPALATIAVLGPLMGLFGTVIGMIEIFASYTPTGGDPAQLARGISIALYNTAFG
                     IIIAIPALIFHRYFRSRVDYYLLRMEREANALNRLINRGTLR"
     gene            2094..>2315
                     /locus_tag="VIR04_07840"
     CDS             2094..>2315
                     /locus_tag="VIR04_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY9460695.1"
                     /translation="MVLTGSESVSGVVLSVAQLNRQVGALLEGHFSQVWVRGEVSNFT
                     QAASGHWYFTIKDERAAVRAVMFRGRAQSV"
BASE COUNT          483 a          615 c          685 g          532 t
ORIGIN      
        1 gcacgcgccc attacccaag ccgcgatcat cttgcaccaa caattcaata tcgcggccta
       61 gcgcccgatg ttgcaggcca tcatcggaaa tgatgacgtt cacatcaggg taggcgcgtt
      121 cgagttcgcg caaagctaaa acgcgctgcg ggtgaacggc caccgggacc tgggccagtc
      181 gcgcaatgag cgcgggttcg tcaccgaact cggcggggtt cagcgcgccc cggccggctc
      241 ttgcctgttt gccgatggcc acccgatagc cccggctaat tacgcccggt tgccagcccc
      301 gcgccttcaa ggcttgcgca agggcgatga caacaggcgt tttgccagtc cctccgacat
      361 agagattacc gattaccagc accgggcgcg agctttgagc ggttgtgcgc cggccacatt
      421 catattggct tttttttcgg gcgatggccc agcgtgcaat ccacgacaag ggcaaaagca
      481 cggtgcttgc aatgcccttg cgttgccata tggtctggat ttggcggatg aggaaaccgt
      541 gtgcgttcaa ggcgagcttt cggtggcaaa attaacccgg ctgatgccgg ccattcgcgc
      601 cgcctgcatc acgcgaacta ccgcctggtg cgtgctttgc gcatccgcgt taatgaccag
      661 aaccgggtcc tttttgtcgt cggcggcggt tcgcagcgcc tgggcgattt cgtcgacgtc
      721 tctgcccggc agcagcaatc cgttgatggc gtacagacca tcctggctca ccgcgacatt
      781 gattgccgtc gtgtccaccg cgtcggcgct tgcttgcgga agcgccactt taagttggtt
      841 cagtcgcgtg aacgaggtgg tcacggcaag gaaaatcaaa ataaccagaa gcacgtcaat
      901 gagcgggatc aggttgatct cgggttcgtc ggcggcgagt cttcggcgaa aattcaacgc
      961 agcgtccctc ggttaatgag gcgattcagg gcattggcct cgcgttccat ccgcagcagg
     1021 taataatcga ctcttgatcg aaagtaacga tgaaagatca gagcgggaat cgcgatgatg
     1081 atgccgaacg ccgtgttata caaggcgatt gaaatgccgc gggccaattg cgcgggatcg
     1141 ccgcccgttg gcgtgtacga ggcaaagatc tcgatcattc cgatgacggt tccaaacagg
     1201 cccatcagcg gacccagaac ggcgattgtc gccaaagcgg gcaagtagcg gttcaattgg
     1261 tgtccgatgt ctttgccaac gtcttcaatg gctgcaacgc gataggcatt ggtctcggcg
     1321 ctattgacca gcacttcggc caatacgcgc ccaaggggcg aactctcggc cagccggtct
     1381 acggcttccg gagtttgctg gttgcgctgg atcaagtccg caacttgcgc ggacagattg
     1441 gccggcatga tcagttttcg tcgcaacgac aggaatcgct cgataatcag cgccaaaccc
     1501 aacaccgatg ttgccagcag cagccagata ggccatcctg ccgcgtcaat gatctgaagc
     1561 aatatcgtct ccgccggata aaataaagtt agaaggcgct attttaaggg caagcctgtg
     1621 tttgcggaca agctgttctc gtttgatgtg cgctccatgt ccgccaaacg cttgtaatct
     1681 cggccggaga gccattggcc gttgcaatgg ggttgcagct tatggcttac gccgtatgtc
     1741 gttaccggca gcgttgaatg gtgcgcgttt gcggttttca aggcctcaaa agagccgcgc
     1801 atgctcaaaa aaagcacgat atgcgcatgg cgccttgctg tcgggacggt atccacagaa
     1861 tctgtggata attgtgtgca caacttgtca gtcgcgccgt atttaacagg gctggcggtt
     1921 cgatggataa tttgagctca ttaataaaat tgtgaatata gttttattta tcagatagtt
     1981 ataatgctaa tttaaagtgt tgtttcatgt ttgcctggaa ttggattgat agttcaacgg
     2041 tttggcgcag cgtgttcttc tgtggacata gttgcttaat tggggcgtga gcgatggtat
     2101 taacgggttc tgagtctgtc agcggggtgg ttttgtcggt ggcgcaatta aatcgccagg
     2161 tgggcgcgct gctcgagggc catttttccc aagtatgggt caggggcgaa gtgtcgaact
     2221 tcacccaggc ggcatccggc cactggtatt tcactatcaa ggacgaacgc gccgcggtgc
     2281 gggccgtcat gtttcgcggc cgggcacagt cggtc
//
LOCUS       DATCXU010000346         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_812980,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000346 DATCXU010000000
VERSION     DATCXU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812980"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VIR04_07845"
     CDS             complement(<1..878)
                     /locus_tag="VIR04_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY9460696.1"
                     /translation="MALKILAVSAEAFPLAKTGGLGDAVSGLCRCLHAIGAHITLLLP
                     AYRETTQHVSGIRQIARLEGLPGGGSASLLAGESKELGLPVYLLKNDALYDRDGIYAN
                     RDGVEYPDNAFRFSALAQVAARIGAGIASVPRPDIVHAHDWHAALTPLYLKQLRVSDV
                     KTILTLHNIAFQGLFPMGLAQSLSIEPRFCYGDGAEFWGQLSFLKAGIRYADLITAVS
                     RNYAREILTPKFGCGLEGVLSERRNDLLAIPNGIDTALWNPQRDPYLSRHSSFSAEKM
                     VNKTIWKRELQRVFGLT"
     gene            complement(1151..2536)
                     /locus_tag="VIR04_07850"
     CDS             complement(1151..2536)
                     /locus_tag="VIR04_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY9460697.1"
                     /translation="MPHLIIIDDEPATRQIIAEIAADEGYVVSQAGNVKQARIQIERQ
                     KPDLVLLDMRLPDGDGIELWTDLALPDTQVVFLTGNPSIESAIAALRCGAVDYLQKPV
                     SLRRLRGILIELRAMTKAPHHAVAANCFAKMIGHSAAIQLLCAHIEKVAPTQATVLLV
                     GESGTGKELAAEAIHLASPRHEKPFMAVNCGAISPNLIESELFGHEKGSFTGADRQHK
                     GYFERASGGTLFLDEITEMSMELQVRLLRVLETGHFMRVGTHEEIEADVRVIAATNRN
                     PEQAVSEGALREDLYYRLSVFPLELPPLRDRGSDVLLIAEHFLQKLNEEGHTQKRFSP
                     VAMQAMQNYLWPGNVRELRNFVYRSYILSDDIINGDFNSFELEMSSSTPDSEILVPVG
                     VPLADANRQLILATLKQCGGVKKLAAEVLGISVKTLYNRLEEYGVSHPEEYGVGANSD
                     ESSALHASENH"
     gene            2758..3717
                     /locus_tag="VIR04_07855"
     CDS             2758..3717
                     /locus_tag="VIR04_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY9460698.1"
                     /translation="MIKTSKFLGRSAAAFQLARSIVKAAPTEASVLLVGESGTGKEVV
                     ARAIHNRSPRRNLPFVAINCGAISPSLVESELFGHEKGSFTGASSTTTGCFERANPGT
                     LFLDEITEMPLAMQVQLLRVLECGEYCRVGGSQQLKVDLRIISATNRDPKKAVAEGLL
                     RSDLLYRLAVFPMRVPPLRERKIDIGYLSQRFLNELNEQEKTAKVFSPQTIAKLESYN
                     WPGNIRELKNTITRAFILADSEVDVDVAPLGLALKSKSLPVRQELIKIPVGTSLMSAQ
                     RAIIMATLDYNSGDRRRTALSLGITEKTLYNRLEQFREHGYAA"
     gene            3926..>4378
                     /locus_tag="VIR04_07860"
     CDS             3926..>4378
                     /locus_tag="VIR04_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460699.1"
                     /translation="MVQLTYEIRARQQTTLTPRLQQSVKLLQMSTLDFSHELAEAIAN
                     NPFLEADDGVSPPAPGETPEAESEPTLNSCPALHGAESADASDDASGGAPPDGPASDG
                     AALADTEFAPGQEIEIQYCDAEQRSADSNYSGDYPPARGVDRPDTDVSQ"
BASE COUNT         1039 a         1227 c         1093 g         1019 t
ORIGIN      
        1 gtcaatccaa agacacgttg cagctcgcgc ttccatattg tcttgttgac cattttttca
       61 gcgctaaacg aggagtgccg cgacagatag gggtcacgct gcggattcca cagcgccgtg
      121 tctatcccgt tcgggattgc cagcagatcg ttgcggcgct cggacagcac cccctcaagt
      181 ccgcaaccaa attttggcgt gagtatttcg cgggcgtagt tgcggcttac agcggtgatc
      241 aggtcggcat accggattcc ggcctttaaa aaacttaatt gtccccagaa ttctgcgcca
      301 tcgccatagc aaaagcgcgg ctcaatgctc aatgactgcg ccaatcccat cggaaacaga
      361 ccttgaaatg cgatgttatg caaagtaaga attgtcttta catcgctgac tcgaagctgt
      421 ttcagataca atggcgtcaa agcggcgtgc cagtcgtgcg catgcacgat atctggccga
      481 ggcacagaag ctattcccgc gccgattctc gccgcaacct gcgcaagagc tgaaaaccga
      541 aaggcgttgt ccggatactc aacgccatcc ctgttcgcgt atatgccgtc gcggtcgtat
      601 aaagcatcgt tcttgagtag ataaaccggc agacccagtt ccttggactc ccccgcgagc
      661 aacgacgccg agccgcctcc cggcaaacct tccagtcttg caatctgacg tatgccgctt
      721 acgtgctgag tcgtttcccg gtaggccggg agcagcaacg taatatgcgc tccgatagca
      781 tgcagacaac ggcaaagccc gctcaccgcg tcccccagcc cccccgtctt ggcaagcgga
      841 aaggcttcag ccgaaactgc caaaattttt agcgccacag tcattctccg ataaccggac
      901 aaactcactg cgccgttcgg ttatctcgtc agcccgattc gattaattca attcggtaaa
      961 aaatgctgcg aagcgaaatt tccacctatg gcgcaatgac gagccctttt tgatttcttt
     1021 cgtctatcgg gattgagcaa gtggcatgcc cagcgaagta ctaatcgtta aattctttgc
     1081 tgcgttattg cgccggtttt gttcccgggg cctgacggaa tatagttcgt tccagaaaga
     1141 gccactattt tcagtggttt tcagaagcgt gcagcgctga agattcgtcg gagttggcgc
     1201 ccacgccata ttcttcaggg tgagacacgc catattcttc cagccgattg tataaagtct
     1261 tgacgctgat cccgaggact tcggcggcca gtttttttac gccgccgcac tgctttaatg
     1321 tggccaggat aagctgacga ttcgcatcgg caagcggtac gcccacaggt accaggattt
     1381 cggaatccgg tgtggaagag gacatttcaa gttcgaatga attaaaatca ccgtttatga
     1441 tgtcgtcgga aagaatataa ctgcgataaa caaaatttcg aagctcgcga acattgccgg
     1501 gccacaaata attttgcatg gcttgcatgg caaccggcga gaaacgcttt tgcgtatgcc
     1561 cctcttcatt gagtttctgt aaaaaatgtt ctgcaatcag caaaacgtcg ctgcccctgt
     1621 cgcgcaacgg aggcaattca agcggaaaca cactaaggcg gtaataaaga tcttcacgca
     1681 aagcgccctc gcttaccgct tgttccggat tgcgattggt ggcggcgata acccggacat
     1741 ccgcctcgat ttcttcgtgt gtgcccacgc gcatgaagtg cccggtttcc aatacgcgca
     1801 acaggcgcac ctgtaattcc atcgacattt ccgtgatttc gtccaggaat agggttccac
     1861 ccgacgcacg ttcaaaatag cctttatgtt gccggtctgc gccggtgaag cttcctttct
     1921 cgtgtccgaa tagctcgctt tcgatcagat tgggagaaat ggcgccgcaa tttaccgcca
     1981 tgaatggctt ttcgtggcgc gggctggcta aatggattgc ttcggcggcg agctccttgc
     2041 ccgtgccgct ctcgcccacc aacagcacag tagcctgggt aggagccacc ttttcaatgt
     2101 gcgcgcacaa taattgaatg gctgcggaat gacctatcat tttggcgaaa caattggcag
     2161 cgactgcatg gtgtggcgcc ttcgtcatcg ccctgagttc aatcaagatg ccgcgcagtc
     2221 tgcgcaaact gacgggcttt tgcaaatagt cgaccgcacc acaacgcaac gccgcgattg
     2281 cgctttcgat actggggttt ccagtaagga acaccacttg cgtatcaggc agcgccaagt
     2341 cggtccagag ttcgataccg tcgccgtccg gcaatcgcat gtcgagaagc accaggtcgg
     2401 gcttttgccg ttcgatttga atccgagcct gcttgacatt ccccgcctgc gatacgacgt
     2461 aaccttcgtc cgcagcaatc tccgcgatga tctggcgtgt ggcaggttcg tcatcgataa
     2521 ttattaaatg tggcatatat cgggcccatt gatgttttct tgacccaaac accgaatcaa
     2581 gagactatcg aattttattt attcgccgca ctttcaaagc caccgtgcgt agtatttatt
     2641 gagaatatcg tacccgtttt tcgtgcacat aagcgtaaat gctgatgtac attgcaacac
     2701 gatattacgt cgtaaaaatg aatagaccca agccaattct cgcattagac tttttccatg
     2761 atcaagacct ctaaatttct gggccggtcc gccgccgcgt ttcaacttgc gcgctcgata
     2821 gtgaaagccg cgcctacaga agccagcgtc cttctcgttg gagaaagcgg caccggcaaa
     2881 gaagtggtgg cgcgagccat tcataatcgc agtccccgcc gcaatttgcc gttcgtcgca
     2941 atcaattgcg gagcaattag tccttctctt gttgaaagtg aattattcgg ccacgagaaa
     3001 ggcagtttca ccggcgcctc atcgaccacc acaggctgct tcgagcgcgc aaatcccggc
     3061 actttatttt tggacgagat cactgaaatg ccgctggcca tgcaggtgca actacttcgc
     3121 gttcttgagt gcggcgagta ttgccgcgtc ggcgggtcgc aacaacttaa ggtggatctg
     3181 agaattattt ccgccactaa ccgcgatccc aaaaaggccg ttgcagaagg ccttttgcga
     3241 tctgacctgc tgtaccggtt ggccgttttt ccaatgcgag ttccaccctt gagagaacgc
     3301 aaaatcgaca tagggtattt atcgcaacgt tttttgaatg aacttaatga gcaggaaaag
     3361 acggcgaaag tattttcgcc ccaaaccatt gcaaagctgg aaagctataa ctggcccggc
     3421 aatatacggg aactcaaaaa caccattacc cgcgcgttca ttttggctga cagcgaagtc
     3481 gatgtggatg tcgccccgct tggcctggcg ctaaaatcca aatcgctgcc agtcaggcag
     3541 gaacttataa aaatacctgt gggcacctcg ctcatgtcgg cgcaaagagc catcatcatg
     3601 gcaacgctcg actacaactc gggcgatcga cgccgcacag cgctgtcgct cggcatcact
     3661 gaaaaaacgc tctataaccg cctcgaacag tttcgcgagc atggctacgc ggcctagtca
     3721 tgcacttctt acacttgtcg gtaatttatc tacaccgtcc gcattcccaa ggcaatggct
     3781 gctaatctgg atctgcgtca aaccgtcatt acccctactg tgcacgttgt tgttcggccg
     3841 tgacaataat tcagcatatc tggcacgatt cttgctttat gtacctgtac tactccatcc
     3901 atagactcga aaacaggaga gaacaatggt gcaactcact tatgaaatcc gggcaaggca
     3961 acagacaact ctgacgccgc gattgcagca atcggtcaag ttgctgcaaa tgtccacgct
     4021 agatttcagc cacgaactcg ctgaggccat cgcgaataat ccgtttctcg aagcggatga
     4081 cggggtttcg ccgcctgcgc ccggcgaaac gcccgaggca gaaagcgagc cgacgttgaa
     4141 ttcgtgcccc gccttgcacg gcgccgaatc ggctgacgcc agtgacgacg ccagcggcgg
     4201 ggcgccgccc gacggaccag cgtccgacgg agcagcgctc gcagatacag aatttgcgcc
     4261 tggtcaggaa atcgaaatcc agtattgcga tgccgagcaa agatcagcgg actcgaatta
     4321 ttccggcgat tatccccctg cccgcggcgt agaccggccc gacactgacg tcagtcaa
//
LOCUS       DATCXU010000347         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_972891,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000347 DATCXU010000000
VERSION     DATCXU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972891"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1857
                     /locus_tag="VIR04_07865"
     CDS             160..1857
                     /locus_tag="VIR04_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY9460700.1"
                     /translation="MRNNQSLAPISRLGGHILVDQLVAHGVKHVFCVPGESYLAVLDG
                     LYDRAIEVTVCRQEGGAAMMAEAHGKLTGQPGICIVTRAPGASNALAGIHIAHQDSTP
                     LVLFVGQIARGMRERDAFQEMDYRAVFGTQAKWVTEIDQIERIPELVSRAFHMATSGR
                     PGPVVVALPEDMLAEYATVADAPHYQVVETSPTAAQMQALADELGRAHRPVAILGGSR
                     WSEQAIAQFAQFAERAQLPVAVQFRRQMLFPTDHSSFVGDVGLGSNPEMMRYIKNADL
                     ILLVGGRMSEIASQSYTLLDIPVPAQRLIHVYPDSAELNRVYRADVAINTSPISFASA
                     LTAVKTSATPAKADQLQTLRSSYLSWTDPNQIKHPGSLQMGQVMAYLNSHLPADTIMT
                     NGAGNYATWLHRFYAFKRYGTQLAPTSGSMGYGLPAAVGAKRLYPDKTVVCFAGDGCF
                     LMHGQEFATAVQYHLPIIVVVIDNGMFGTIRMHQERHYPGRISATSLHNPDFSAYAIA
                     FGGYGERVETTEQFGPAFERALASKKPAILHCLIDPETISPTTTLEKIRERALSAKH"
     gene            2035..2985
                     /locus_tag="VIR04_07870"
     CDS             2035..2985
                     /locus_tag="VIR04_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460701.1"
                     /translation="MIALAGSMSAHAATYPERNITLLVPYPPGGTTDISARAFAHVLQ
                     GELKQSVIVENRGGAGGVIGMGVVAHAKPDGYTLGLGTIGTQSINQFMYKKMSYDPST
                     AFVPIALVITTPNVIGVPASSSIKTLADLIATAKAKSAKGDKLSYASPGVGSSVHLTG
                     AYFEQVAGIDMLHVPFKGGAESITAVSGGHVDVLFDNLPSSLNQIKDGSKIRGLAVTS
                     SQRSPAIPNIPTVAESGLPGFDVTAWFGLWAPKGTPQAVIDTLIKASRQALKSPELNA
                     TFAAVGAQAGTLFGADLGAFEQSERDRWSKLIADRHIEAK"
     gene            complement(3021..3623)
                     /locus_tag="VIR04_07875"
     CDS             complement(3021..3623)
                     /locus_tag="VIR04_07875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY9460702.1"
                     /translation="MSAPIIYLASASPRRHEILQQMNVLHQVLRVPAAPGEDEPRLEN
                     ETPERYVRRTARDKATRATEWILAQSLSHWPVLSADTTVILDQDILGKPADCGDAAQM
                     LGRLSGRTHSVHTAIALAHQGSLLEDVSVTAVRMKKLSAAEIDAYCKTGEPLGKAGSY
                     GIQGYAARFIEHISGSYTGVMGLPIFETHRLLQAAGLLQS"
     gene            complement(3620..>4087)
                     /locus_tag="VIR04_07880"
     CDS             complement(3620..>4087)
                     /locus_tag="VIR04_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEY9460703.1"
                     /translation="PVEAVAAMARICLGAEQEPMTTQSKHRLGETFSRCDETIALSSM
                     YAANHFPGIKAIISLTESGHTPLIMSRIRSGVPIYCYTPHANTQRRAALFRGVYTVPF
                     DASEMDPTTVSDNAINKLKQRGLLKKGDWIILTKGDYYSDSGGTNGMKILKVK"
BASE COUNT          841 a         1153 c         1189 g          904 t
ORIGIN      
        1 ccccgcgcct caccgcccgg gggcgttgct gttgtggccc ccacaccccg aggccgcgcc
       61 gcctgagccg tgcgcgcgca tgacccggaa agccggaatg agatatgatg cggatctgtt
      121 gacgtattgc gcttaatcca catttcattt cggacaacaa tgagaaacaa tcaaagcttg
      181 gcgcccattt ctcgtttggg cggacacatt ctggtcgatc aacttgtcgc tcacggcgtc
      241 aagcacgttt tctgcgtgcc gggggaaagt tacctggcgg tgctggatgg cttgtacgat
      301 agggcaatag aggtcactgt ttgccgccag gaaggcggcg cggccatgat ggcggaagca
      361 catggcaaac tgaccggcca gccaggcatc tgcattgtta cccgagctcc gggagcgtcc
      421 aatgcgctgg cggggataca tatcgcccat caggattcca ctcctcttgt tttatttgtc
      481 ggccagatcg cacgcggcat gcgcgagcgt gatgcattcc aggaaatgga ctaccgcgct
      541 gtttttggca cccaagccaa gtgggtcacg gaaattgatc agatagagcg cattccagag
      601 ctggtttcgc gggcgtttca catggcgacg tcggggcgcc cgggccctgt cgtggttgcg
      661 cttccggagg atatgctggc cgaatacgcc actgtcgcgg acgcgccgca ctatcaggtg
      721 gtcgaaacat cgcccaccgc cgcgcagatg caggcgcttg cggatgaact cggccgcgcg
      781 cacaggccgg tggccatttt gggtggttcg cgctggagcg aacaggctat tgcccagttt
      841 gcgcaatttg ccgaacgcgc ccagttgccc gttgctgttc agttcagaag gcagatgctg
      901 tttccgaccg atcactccag ctttgtcggc gatgtcggtt tgggcagcaa ccctgaaatg
      961 atgcgctaca tcaagaacgc ggatctgatt ctgctggtcg gcggacgaat gtcggagatc
     1021 gccagccaaa gctacacatt gcttgatatc cctgttcccg ctcaacgtct catccatgta
     1081 tatcctgaca gcgccgagct taatcgggtt tatcgggccg acgttgcgat caacacctcg
     1141 ccgatctcgt ttgccagcgc gctgacagcg gtgaaaacaa gcgcaacccc ggcgaaggcg
     1201 gatcaattgc aaacgcttcg ctccagctat ctgtcatgga ccgatcccaa tcaaatcaag
     1261 catcccggat cgctgcaaat gggccaggtc atggcgtacc tgaattcgca tttgccggcc
     1321 gacacgataa tgaccaacgg cgctggaaat tatgcaacat ggctgcatcg tttttacgcc
     1381 ttcaagcgct acggcaccca attggcgccg acctcgggat cgatgggtta tggcctgcct
     1441 gcggccgtag gcgccaaacg gctttatccc gacaaaaccg tcgtttgctt tgccggcgat
     1501 ggctgttttc tgatgcatgg acaagagttc gccactgccg tgcaatacca tctgcctatt
     1561 atcgttgtgg taatcgataa tggcatgttt ggcacgattc gaatgcacca ggaacgtcat
     1621 taccccggtc gcatatcagc gacgtccttg cacaaccctg atttttccgc atacgcgatt
     1681 gcgtttggcg gctacggaga acgtgtggaa acgacggaac aattcgggcc tgctttcgag
     1741 cgtgcgttgg caagcaaaaa gccggctatc ctgcattgcc tgatcgatcc ggaaacgatc
     1801 tcgcccacaa cgactcttga aaaaattcgc gagcgggccc tttcggcaaa gcactgaaat
     1861 ggccgcgcgg ccgtctgata tcgtaaaaga ccccggctcg gaagcaatct ttgcgtatcc
     1921 gaaagatttc taattcgcgt catggcacta cattgtatgg aaacaaagag gagatagtta
     1981 atgaagcagt caagccgttt tttcaagacg ttcaagccat tcgcctatgc ggcgatgatt
     2041 gccctggccg gttccatgag cgcacacgcg gcaacgtacc ccgaacgaaa catcacgctg
     2101 ctggtgccgt atccgccggg aggcacgacc gatatttcgg ctcgggcatt cgcccacgtc
     2161 ctgcaaggcg agttgaagca atccgttatt gtcgagaatc gcggtggcgc cggcggcgtt
     2221 attggaatgg gcgtcgtggc ccacgccaag ccggacgggt atacgctggg tctgggaacg
     2281 ataggcacgc aaagcatcaa ccagttcatg tacaaaaaaa tgtcctacga cccgtcgact
     2341 gcttttgtgc cgattgcgct tgttatcacc acgccgaacg tcatcggcgt gcccgccagt
     2401 tcaagcataa agacgctggc cgatcttatt gcgaccgcaa aagcaaaatc agccaaaggc
     2461 gacaagctat cctatgcgtc cccgggggtc ggttcatcgg tgcatcttac cggagcttac
     2521 tttgaacagg tcgcgggcat tgacatgctg catgtgccat tcaagggcgg cgccgaatcc
     2581 ataaccgcgg tgagcggcgg ccatgtggat gttctgttcg ataatttgcc gagttcattg
     2641 aatcagatca aggatggttc caagatacgc gggcttgcgg tgacatcgtc tcagcgctcg
     2701 cctgccatcc cgaacattcc cactgttgcc gagtcgggtt tgcctggttt tgacgtaacg
     2761 gcatggtttg gcctttgggc ccccaaggga acgcctcagg cggtcatcga taccctgatc
     2821 aaggcatcca gacaggcgct caagtcgccc gaactcaacg ccacctttgc ggcggtgggt
     2881 gctcaggccg gaacgttatt tggagcggat ctgggcgctt ttgagcagtc ggaacgggat
     2941 cgctggagca agctgattgc ggatcggcat attgaagcga agtaggcagc gcgcggcgaa
     3001 cctcgccgcg cgtgggctcg tcaagattgc aacagacccg ccgcctgcaa caggcggtga
     3061 gtctcgaata tgggcaggcc catgacgccc gtgtaactgc ctgaaatgtg ctcgatgaat
     3121 cgcgctgcgt agccttgtat gccgtaactg cccgccttgc ccagtggctc gccggtcttg
     3181 cagtaagcgt cgatttctgc tgcggataat ttcttcatgc gtactgcggt tacggatacg
     3241 tcttccaaaa ggctgccctg gtgcgccagg gcgatggctg tatgcaccga gtgggttcga
     3301 cccgacagcc gtcccagcat ttgcgcggca tcgccgcaat cggccggttt gcctaaaata
     3361 tcctgatcga gaataacggt ggtgtccgcc gataaaacag gccaatggga cagcgactgc
     3421 gcgaggatcc attctgtggc gcgcgtggct ttatcgcgcg cggtgcgacg cacatagcgt
     3481 tcaggcgttt cgttttccag gcgcggctca tcttcgccgg gcgctgcggg cacccgcaac
     3541 acctgatgca aaacattcat ctgttgcaat atttcatgcc ggcgcgggct tgccgacgcc
     3601 agataaatta ttggcgcgct catttgacct taaggatttt catcccgttg gtgccgccgc
     3661 tatcgctgta ataatcgcct ttggtaagga tgatccagtc gccttttttc aaaagccccc
     3721 gctgcttcag cttgttgatg gcgttatcgc tgacagtggt cggatccatt tccgatgcgt
     3781 caaagggcac cgtgtaaacg cctcggaaca gggcggcgcg acgctgcgtg ttggcatgag
     3841 gcgtgtagca gtaaatgggg acgccggagc gaatgcgcga catgatcaaa ggagtatgtc
     3901 cgctttcggt caagctgatg attgctttga tgccggggaa atggttcgcg gcatacatgc
     3961 tggacagggc gatagtttcg tcgcaacgac tgaaagtttc tcccaggcgg tgcttggatt
     4021 gcgtcgtcat gggttcctgt tcggctccaa ggcaaatgcg cgccattgcc gcaaccgcct
     4081 cgaccgg
//
LOCUS       DATCXU010000348         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_304519,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000348 DATCXU010000000
VERSION     DATCXU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304519"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VIR04_07885"
     CDS             complement(<1..1364)
                     /locus_tag="VIR04_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY9460704.1"
                     /translation="MDSFAKETLPVSLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELNNDWNRAYKKSARIVGDVIGKYHPHGDAAVYETIVRMAQDFSLRYML
                     VDGQGNFGSIDGDSPAAMRYTEIRLSKIAHEMLADIDQETVDFGANYDGSEQEPLLLP
                     SRLPNLLVNGSSGIAVGMATNIPPHNLAEVVDGCLYCLRNPDCTIDELIEIIPAPDFP
                     TGGIIYGMSGVREGYRTGRGRVIMRAKTHFEDMEKGHRHAIVVDAIPYQVNKKSLQER
                     IAELVNEKKIEGISDIRDESDKDGMRLVIELKRGEVPEVILNNLFKNTQLQDTFGMNM
                     VALVDGQPRLLNLKQMVEYFLFHRREVVTRRTVFQLRKARERGHVLEGLAVALANIDE
                     FIAIIKAAPTPPVARQDLMARTWDSSLVREMLSRADDGNTPGGSAAYRPEGLAEGFGL
                     QSDG"
     gene            1782..2369
                     /locus_tag="VIR04_07890"
     CDS             1782..2369
                     /locus_tag="VIR04_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY9460705.1"
                     /translation="MNKPSKIALAIATVAFTASGALSAQTIDNWTNPYGNVWKNGTNE
                     LCWRDNFWTPATGIAGCDGVPVAQAAQAPVTAPTATKVVLNADTFFDFDKSTIKPEGR
                     KILDQVASQAESINLETLIATGHTDSIGTEKYNQKLSERRANAVKKYLVSKGIDANHI
                     YAEGKGELSPVATNKTREGRAKNRRVEIEIVGTRK"
     gene            2459..3193
                     /gene="ubiG"
                     /locus_tag="VIR04_07895"
     CDS             2459..3193
                     /gene="ubiG"
                     /locus_tag="VIR04_07895"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY9460706.1"
                     /translation="MTLPDSTVPVTPSNADQAELDKFSALASRWWDPESEFKPLHAIN
                     PLRLGWIREQAGSLANKRIVDVGCGGGILAESMARAGARVTGIDLAEKSLKIARLHGL
                     ESGVTVDYQAISAEHLAEQQPGQFDIVTCMEMLEHVPDPVSTVKACSKLVKSGGWVFF
                     STLNRNPKSFLFAIVGAEYLLRMLPRGTHNYENFIKPSELAAAARQNRLDVTKLSGLR
                     YNPIVDHYTLSDDASVNYLLATRRAA"
     gene            3220..>3791
                     /locus_tag="VIR04_07900"
     CDS             3220..>3791
                     /locus_tag="VIR04_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY9460707.1"
                     /translation="MRGLVLFDFDGTLADTAPDLAAAANKMRVRKGLAPLPYEALRPL
                     ASQGARGLLKAGFDMNPDDPGYETHRRQFLQDYEQDMTSLTTLFPGVARLLSTLKQND
                     YAWGIVTNKVSYLALPIVRHLGLQTECAITVGGDTASHPKPHPAPLLYAAAQTGFMPQ
                     QCLYIGDDERDIIAGKAAGMPTIAAAYGYCG"
BASE COUNT          918 a         1124 c          956 g          793 t
ORIGIN      
        1 aacccgtcag actgcaagcc aaacccttca gccaggcctt ccggtctgta cgcggcgctg
       61 ccgccagggg tattgccatc atcggcgcgg cttagcattt cgcgcaccag cgaagaatcc
      121 cacgtccggg ccatcaagtc ctggcgggcc acgggcggcg tcggagccgc cttgataatg
      181 gcgataaatt cgtcgatgtt ggccaaggca accgccaggc cttcgagcac atggccccgt
      241 tcgcgcgcct tgcgcaactg aaatacagtg cggcgcgtaa cgacctcgcg acggtgaaac
      301 aaaaagtatt cgaccatctg cttcaaatta agcaaacgcg gctggccatc gaccagcgcg
      361 accatattca tgccgaaggt gtcttgaagc tgagtgttct tgaacagatt gttcaatatg
      421 acttccggaa cttcgccgcg cttgagctcg ataaccaaac gcatgccgtc cttatcggac
      481 tcgtcccgaa tatctgaaat accctcgatt ttcttttcgt taaccagttc cgcgatgcgt
      541 tcctgcaacg attttttgtt tacctggtag gggatggcgt cgacaacaat cgcatggcgg
      601 tgtccctttt ccatgtcctc gaagtgcgtt ttcgcccgca tgatgacccg gccgcggccc
      661 gttcgatacc cttcgcgcac accggacatg ccataaatta ttccgcctgt cggaaaatcc
      721 ggagcgggga taatttcaat aagctcgtcg atcgtgcaat ccggattacg caaacaatac
      781 aaacatccgt cgaccacttc agccaggtta tggggcggaa tattcgtcgc cattccaacc
      841 gcgatccctg aactgccgtt caccagcaga ttgggcaaac gggaaggcag aagcaagggt
      901 tcctgctcgc taccgtcgta attcgcgcca aaatcgaccg tctcctggtc gatatcggcc
      961 agcatttcgt gggcgatttt cgaaaggcga atttcggtat aacgcattgc cgccggactg
     1021 tcgccatcga tagacccaaa gttgccctgt ccatcgacca acatgtaacg cagcgaaaag
     1081 tcctgcgcca ttcgcactat cgtttcatat accgccgcat cgccgtgcgg gtgatacttc
     1141 ccgataacgt ccccgacgat acgcgccgat tttttgtagg cccggttcca atcgttattt
     1201 agttcgtgca tggcgaacag cacacgtcga tgcacgggtt tgaggccatc acgcacatcg
     1261 ggcagagccc gcccgacaat cacgctcatg gcgtaatcca gataactgcg gcgcatctcg
     1321 tcttccagcg aaactggaag ggtctccttg gcaaaggaat ccataaactc tctagtactt
     1381 gaaatcgtga aggcgggaca gccacgggcg aataggaaat tctatcactt ccgcccataa
     1441 tgcccggccg gcaataaaac agccctgtca tggaatgcca aaacaagcct gaacggctgg
     1501 tttgcgaaga cgtcttgaga aaccacttta agcgtaggtt atgttgccta aaaccaacaa
     1561 taatgccaaa gatcacgtag aaataccgat atctaagcca tatacgcaaa acgcgcttaa
     1621 ctaacccatc ataaatgccg taagattcgc cctaacacgc atgaaaccca gcgcaatact
     1681 ttgagttcac tcatggcatt gctatactgg ctccgttttc ttgatatgcg gcgggggttc
     1741 gctgcggtca cttaccagca tttagctcaa cgaggagaaa catgaacaaa ccctccaaaa
     1801 tcgcattagc aatcgccact gttgccttca cggcatctgg cgcactatct gcgcaaacga
     1861 tcgacaactg gactaaccct tacggcaacg tctggaagaa tggcactaac gaattgtgct
     1921 ggcgcgacaa cttctggacg ccagccaccg gcatcgccgg ttgcgacggc gtacccgttg
     1981 ctcaggcggc tcaggctcct gtaacagcgc ctacggccac caaagtcgtt ctgaatgccg
     2041 atacattctt cgattttgac aagtccacca tcaaacctga aggtcggaaa atccttgacc
     2101 aagtcgcttc gcaagccgaa tcgatcaatc tggaaactct gattgccaca ggccatactg
     2161 actcaatcgg tacagagaag tacaaccaaa agctttctga acgtcgcgca aacgccgtca
     2221 agaaatactt ggtcagcaag ggtattgacg caaatcatat ttacgctgaa ggtaaaggcg
     2281 agttgagccc tgttgccacc aacaagactc gcgaaggacg cgcgaagaat cgccgcgttg
     2341 aaatcgagat cgtcggtact cgcaagtaac aaccggtagg cggcgtcagc cgcgttagac
     2401 ggtacaataa ggggctttgc catgcagagc cccttgtcta ttttgcccgc ggttcataat
     2461 gaccttgccc gattccaccg tacctgtcac accctctaac gccgaccagg cggaacttga
     2521 taagttcagc gccctcgcgt cgcgctggtg ggacccggaa agcgagttca agccgcttca
     2581 tgccattaat cctttaaggc tggggtggat ccgtgaacag gctggttcgt tagccaacaa
     2641 aaggattgtc gacgtcgggt gcggcggcgg tattctggcc gaaagcatgg cacgcgccgg
     2701 ggcgcgggtt accggaatcg acctggctga aaaatcattg aaaatcgcac gcctgcatgg
     2761 cctggagtcc ggagtgacag tcgactatca agccatcagc gccgaacacc tggccgagca
     2821 acaacccggg caattcgata tcgtaacctg catggaaatg ctcgaacatg tgccggaccc
     2881 agtctcgaca gtgaaagcct gcagcaaact ggtcaaatca ggcggttggg tctttttctc
     2941 gactctcaat cgaaatccga agtcatttct atttgccatc gtcggcgccg agtatctgtt
     3001 acgcatgctg ccacgcggca cacacaacta cgaaaacttt atcaaaccca gtgaacttgc
     3061 cgcagccgcg cgtcaaaaca ggcttgacgt gaccaagcta agcggcctgc ggtataaccc
     3121 catcgtcgat cattacacgc tctcggacga cgcgtcggtc aattatctgc tggccacccg
     3181 gcgcgccgcc tgatccattc aaaaataagg ataactccca tgcgtggact cgttttattc
     3241 gactttgacg gcacgctggc cgacacagcg cccgatcttg ccgccgccgc caataaaatg
     3301 cgcgttcgca aaggtttggc gccgctgccc tatgaagcgc tgcgtccgct tgcttcgcag
     3361 ggcgcccgag gcctgcttaa ggcgggtttc gacatgaatc ccgacgatcc cggctacgaa
     3421 acgcatcggc gccagttcct gcaagattac gagcaggaca tgacgtcgct cactacccta
     3481 tttccgggag tcgccaggct gttatccacg cttaaacaaa acgactatgc ctggggcatc
     3541 gtaaccaaca aagtcagtta cctggcgttg cccatcgtaa ggcacctggg cctgcaaacc
     3601 gagtgcgcaa ttacggtcgg cggggacacg gccagccacc ccaaaccgca ccccgctccc
     3661 ctgctgtatg cggcggcgca aaccggcttc atgccacagc aatgtcttta tatcggcgac
     3721 gatgaacgcg acatcattgc cggcaaggcg gcggggatgc caaccatagc cgcagcctac
     3781 ggctattgcg g
//
LOCUS       DATCXU010000349         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_30007,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000349 DATCXU010000000
VERSION     DATCXU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30007"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VIR04_07905"
     CDS             complement(<1..292)
                     /locus_tag="VIR04_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460708.1"
                     /translation="MKCLANLVSRALDAHSTWKVVIATFLWLAATTWTRPLLLPDEGR
                     YVGVAWSMLDVGQFSVPRLDGLPFFHKPPLFYWITALAMQCFGANEWAARLSS"
     gene            complement(276..1229)
                     /locus_tag="VIR04_07910"
     CDS             complement(276..1229)
                     /locus_tag="VIR04_07910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY9460709.1"
                     /translation="MSCVIPCLNEAANLSVLLPELMGVLESMSMRHEIIIVDDGSTDE
                     TASAVAQWAEKHAEIVYIQLSRNFGKEAALSAGLAAAHGRVVVSLDADLQHPPALIPE
                     MLARWQQGADMVYAVRATRADESIFKRVGTKIFYQMMRSSGGLKVPENAGDFRLMDRV
                     VVDALLMLPERNRFMKGLFAWVGFRAEPFYYSPPERLHGTSRFKPFKLFRFALDGVTA
                     FTTWPLRLLSLSGIGLSLISFAYGIFIVVSHWLYGDPVQGWATLITVVLFFAGVNLIS
                     IGVVGEYVARIFGEVKNRPIYLVRKRVGQGIGEKSFNEMPR"
     gene            1547..1951
                     /locus_tag="VIR04_07915"
     CDS             1547..1951
                     /locus_tag="VIR04_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY9460710.1"
                     /translation="MTAQELCSEEEIVTLVYGFYDRVRADALLAPIFNAHVDDWDTHL
                     AKMVRFWSSMLLGAGTYRGSPMPAHIALPGLRADLFQQWLGLFHQTAQELPNRRLAER
                     AEQYAHRIARSLWYGYQLNHEPDQRPTEVTCG"
     gene            1944..3206
                     /locus_tag="VIR04_07920"
     CDS             1944..3206
                     /locus_tag="VIR04_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY9460711.1"
                     /translation="MANSTQIEKQAASPDTRPQLQAFLSLGFRPLYIAGCGWALFSIA
                     IWIFAPHLIGHPLSGVAWHAHEMLWGFIGTIAVGFLLTASATWTKFTPLKDWPLGVVC
                     LLWLCARIGYLAGGEAGFWIAGASELAFYVISAIALMRVVLKAKSRRNYGVPLLVLGL
                     GLTSVLYLQAALKGDYVLLMQRFDLGLIDMAVLALLIARRVVPFFAMRMVAGLKIPML
                     TRSGHVQLTLSVLAIILGLAGLPVIMAIALALVGLISLYQVARWQPTAVLHRPILWIL
                     YLGYALLGIGLLFAAIQLSGLGSGLLARTATHVHIIAMGGFSILIIGMVTRTALGHLG
                     HKLVLDQSMRVSYFFMIAAVALRLAALWPSSASAGLLDAAATCWIVALGLYLWRFTPM
                     LIRPRFQAKASAIPAPAKTPAPSGAPAK"
     gene            3218..3715
                     /locus_tag="VIR04_07925"
     CDS             3218..3715
                     /locus_tag="VIR04_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY9460712.1"
                     /translation="MRLTVMTDYAIRLLMYVGRQPDRLCTISEIAHAYGMSEPHLMKI
                     THRLAQNGWIETVRGKNGGMRLAHAPEDISLGAVMRDTESDFTLVECFVADNTCPLSG
                     HCRLTGIIDGALQRFLQYVDSFTLADILPEAGQAPRKGAVTNLAPPRIGDSARRSASP
                     EKIPG"
BASE COUNT          926 a         1197 c         1086 g          849 t
ORIGIN      
        1 ccgaactcag gcgggcggcc cactcattgg cgccgaaaca ctgcatggct aatgcggtga
       61 tccagtaaaa caggggcggt ttatggaaaa aaggcaagcc gtccagccgc ggcacggaaa
      121 actgcccgac gtcaagcatg ctccaggcca cgcccacgta gcggccttcg tcgggcaaaa
      181 gcaaggggcg agtccatgta gtcgccgcca gccacaagaa ggtcgctata acgaccttcc
      241 acgtcgaatg cgcgtcaagc gccctggaaa ccaggttagc gaggcatttc attgaatgat
      301 ttttcgccta tgccctggcc gactcgtttt ctgacgagat aaatggggcg atttttaacc
      361 tcgccgaaaa tgcgcgccac atattcgccc acaacgccaa tagaaatcag attcacaccc
      421 gcaaaaaaca acacgaccgt aatgagcgtt gcccatcctt gcaccgggtc gccatacaac
      481 cagtgactga caacgatgaa aatcccgtaa gcaaacgaaa tcagcgacaa gccaataccc
      541 gacagactta acagacgcaa gggccatgta gtgaaggccg ttacaccatc aagcgcaaag
      601 cgaaataatt tgaagggttt aaaccgcgac gtgccatgca aacgttcagg cggagagtaa
      661 tagaaaggtt cggcccgaaa tccgacccag gcgaacaacc ctttcataaa acgattgcgc
      721 tcgggcagca ttagcagagc atcgaccacc acccggtcca tcagtcgaaa atcgcccgcg
      781 ttctcgggca ccttcaatcc accggaactc cgcatcatct gataaaaaat cttggtgccc
      841 acccgcttga aaatcgattc gtccgctcgt gttgcccgca cggcatacac catgtcggcg
      901 ccttgttgcc agcgcgccag catttcgggg atcaacgcag gaggatgctg caaatccgcg
      961 tccaggctta cgaccacgcg gccatgggcg gccgcaagtc cggcgctaag tgccgcttcc
     1021 ttgccaaaat tgcgcgacaa ttgtatgtaa actatttctg cgtgcttttc cgcccattga
     1081 gctaccgcgc ttgcggtttc atccgtgctg ccgtcatcga cgataatgat ttcgtgccgc
     1141 atggacatcg attccagcac gcccattagt tcgggcaaca agacacttaa attcgctgct
     1201 tcattcagac acggtatgac gcaactcatt gatgcgggcg gtaacgcgtt gcggggcgtc
     1261 aatgcgggaa tatcgctctt gggttcagcg ctgacactag acaattcggg tcccttcaaa
     1321 atcaagaatc aaaaacaatg cgcgatgcaa ctgggaataa tagctgatta gcccttgcca
     1381 taccgtaaaa aacggcctca tcacagcggt aaaaacgcac tacgaattga cggagcatcc
     1441 gttttttaat aagatgtaaa cgacatatat ctttaaacca agcgggtttt caccttggtt
     1501 tcgcccaatg gctccgcccc gccctcgtct catattggaa tattacatga cggcccaaga
     1561 actatgttct gaagaagaaa tcgtcaccct ggtctatggt ttctacgatc gcgtacgcgc
     1621 cgacgcactc ttggcgccca tctttaacgc gcatgtcgat gactgggata cccatttggc
     1681 caagatggtg cgattttggt cttcgatgct gctgggcgcg ggtacttacc gcggatcgcc
     1741 catgcccgcg catatcgcac tgccaggact gcgcgccgac ctcttccagc aatggctggg
     1801 actatttcat caaaccgcgc aggagctgcc caaccgccgc ctggccgagc gcgccgagca
     1861 atacgcgcac cgcatcgcgc gcagcctgtg gtacggctac cagttaaatc acgaacccga
     1921 ccagcggccc acggaggtca cttgtggcta atagcactca aatcgaaaag caagccgcaa
     1981 gccctgacac tcgcccgcag cttcaggcat tccttagcct gggattccgc cccttgtaca
     2041 tagcggggtg cggctgggct ttgttctcga ttgcgatctg gatcttcgcg ccgcatctga
     2101 tcgggcatcc gctgtcaggc gtagcctggc acgcgcacga aatgctgtgg ggattcattg
     2161 gcaccatcgc cgtcggtttt cttttgacag ccagcgcaac ctggacgaaa tttactccat
     2221 tgaaagactg gccgctgggc gttgtatgcc tgctctggct gtgcgcccgt atcggctacc
     2281 tggcgggcgg cgaagcaggc ttctggatcg ccggcgcgag cgaactggcg ttttacgtca
     2341 ttagcgcgat cgcgctgatg cgcgttgtcc ttaaagccaa aagtcgccgc aactatggcg
     2401 tccctttgct ggtgctggga ctggggctga ctagcgtgct gtatcttcag gcggctttga
     2461 agggcgatta cgtcttgcta atgcaacgtt tcgatctggg cctgatagat atggcggtgc
     2521 tggccctgct gattgcacgc cgggttgttc cattttttgc catgcgcatg gtggcggggc
     2581 tgaaaatccc catgctgacc cgcagcggac atgtgcaact cacattaagt gttttagcca
     2641 tcatcctggg cctcgcgggg ttgcccgtca taatggcgat tgcactggcc ctggtagggc
     2701 tgatcagttt gtatcaagtt gcccgatggc agccaacggc agtccttcac cggccgatat
     2761 tatggatcct gtacctgggt tatgcccttc ttggcattgg cttgctcttc gccgcgatcc
     2821 agctcagcgg cctgggctcg gggctgctgg cgcgcactgc aacgcatgtg cacatcattg
     2881 ccatgggcgg cttttcaata ttgatcatcg gcatggtgac gcgcaccgcg ctcgggcatt
     2941 tggggcacaa actggtgctt gatcaaagca tgcgcgtcag ttactttttc atgatcgcgg
     3001 ctgtcgcgct acgtctggcg gcgctatggc caagcagcgc cagtgcgggg ttgcttgacg
     3061 ccgccgccac atgctggatt gtcgctctcg gtttatatct gtggcgattc acgcctatgt
     3121 tgatccggcc gcgattccag gcaaaggcaa gcgccattcc cgctccggcc aagacgcccg
     3181 cgccatccgg cgcgcccgcc aaataaaaga caaaaacatg cgcctgaccg taatgaccga
     3241 ctatgcgata cggttgctga tgtatgtcgg gcggcagcct gatcgcctat gcacgatctc
     3301 ggagatcgcg cacgcctacg ggatgtccga accgcatctg atgaaaatta cgcatcgcct
     3361 ggcgcaaaac ggctggatcg aaacagtgcg tggcaaaaac gggggcatgc gccttgcgca
     3421 cgcccccgaa gacatcagcc tgggcgctgt catgcgcgat actgaaagtg atttcaccct
     3481 ggtcgaatgt ttcgttgccg acaatacatg cccgctaagc ggacattgcc gtcttacggg
     3541 gatcatcgat ggtgcgctac aacgctttct gcaatatgtc gatagcttca cactggccga
     3601 tattcttcct gaagccggcc aggcgccacg taaaggtgcg gtgactaacc tggctccgcc
     3661 ccgcatcggt gactccgccc gcagatctgc cagccccgaa aaaatacccg gataattcac
     3721 ggccatttca cggccaaaac cgccgtgaac gcgaaagtta ccccattcca tggttaacac
     3781 cgtcgtgaac gcaaaggcca agcgcagaca gtaccaaaga tacggcaagc aacgccgaca
     3841 aagtgcactg cgattttaac ccgcattcca tggttaaaac cgtcgtgaac gcgaaggcta
     3901 agcgcaggca gtaccaaagg tacggcaagc gacgccgaca aagtgcacgg cgattttaac
     3961 catggaatgt ttgtgaaacc cacaggccga ccatatccca cgtgcgtccc acaaagccgg
     4021 caggctcgac gccggacaag gtgaccagag gaagttcg
//
LOCUS       DATCXU010000350         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_819191,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000350 DATCXU010000000
VERSION     DATCXU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819191"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VIR04_07930"
     CDS             complement(<1..566)
                     /locus_tag="VIR04_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY9460713.1"
                     /translation="MGSALKAAGPQRGRSRARLTRWIRKTHGWIGLWGALLGLLFGVS
                     GIWLNHRAILKLPAGAQHRATMQIALPDPVPATPQAMGRWLQSALQLDGPAPSIRVEP
                     ARPAPWVDHTAPSSMEQPPLMQPEHWSFRFGGPQSLILADSWAGNRSISVHRSDNGLL
                     NTLMNLHKGVGMTAAWILLVDTLAGSLI"
     gene            complement(551..1237)
                     /locus_tag="VIR04_07935"
     CDS             complement(551..1237)
                     /locus_tag="VIR04_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY9460714.1"
                     /translation="MLITIPNVLDAQAVEAVRNHLDQAGTAWVDGRATAGYQGAPVKF
                     NRQIDEQSEVARICQRIILDVLQRHPLFISATLPNMVYPPMFNRYDEGMTFGAHVDGS
                     FRIHPQTGQKLRTDVSATLFLTDPGDYDGGELQVEDTYGSHQVKLDAGDMVVYPATSV
                     HQVTSVTRGMRIACFFWVQSLVADDGRRSLLFDMDNAIQRLNQTEADAAARRTLVGCY
                     HNLLRQWGQP"
     gene            complement(1266..>2307)
                     /locus_tag="VIR04_07940"
     CDS             complement(1266..>2307)
                     /locus_tag="VIR04_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY9460715.1"
                     /translation="NQGYYRNGTCNGVPLTAAGATSGYVDCVPLLNPGYTSSPSNAPS
                     LPGNLAAGSATTIAAYISDTVELSPQFKLVGGMRYDHYAATISNSINSSNTVRNTTLA
                     SANQTVNLASWRAGALWQPTGAQSYYVSYSTSFNPSLEQLTSTTGLSQPLAPEKNKSY
                     ELGGKWDLLDGDLSLDSALFQITQYNSRSQNADNTYTANGTIRVNGVRASATGRITEK
                     WRVFGGYTHMNATIVDAISAGTQGMTPANTPRDSASLWTTYRFLPHWEIGGGVTYVSS
                     RFANNNNLVRVGRYARTDATLAYHQSSYDIRLNVFNLTNANYYDALIQSDGGRAVPGS
                     GRTVMLTFAYRM"
BASE COUNT          470 a          639 c          707 g          491 t
ORIGIN      
        1 aaaatcagcg atccggccag cgtgtcgacc agcaggatcc atgccgccgt cattcccacg
       61 cccttatgca ggttcatcag cgtattcaag aggccattgt cgctacggtg tacgcttatt
      121 gaacggttgc ccgcccagga gtctgccaaa ataagggact gcgggccgcc gaacctaaag
      181 ctccagtgtt caggttgcat taacgggggt tgttccatcg acgaaggcgc ggtatggtcg
      241 acccacggcg cgggcctggc cggctcgacc cgtatggagg gcgcagggcc gtccagttgc
      301 aatgcgcttt gcaaccagcg acccatcgcc tgcggggtgg ccggcacagg gtctggcaag
      361 gcaatctgca ttgtggcgcg gtgttgcgcg ccggctggca gctttagtat cgccctgtga
      421 ttcagccaaa tgccgcttac gccaaacagc aaaccaagaa gtgcacccca aagaccgatc
      481 cagccatgag tcttgcgtat ccagcgcgtc aagcgggcgc gggaacggcc acgctgcggc
      541 cccgcagcct tcaaggctga ccccactgac gcagaaggtt gtgatagcag ccgaccaacg
      601 tgcggcgcgc ggcggcatca gcctcagtct gattcaggcg ctggatggca ttgtccatat
      661 cgaaaagcaa cgaacgccgc ccatcatcgg ccaccaggct ttgcacccag aaaaaacagg
      721 cgatacgcat gccacgggtg acggaggtga cctggtgcac gctggtggcc ggatagacga
      781 ccatatcgcc cgcgtcaagc ttgacctggt gcgagccgta agtgtcttcg acttgcagct
      841 cgccgccgtc gtagtcgccg gggtcggtaa ggaacaaggt ggcggacaca tccgtgcgca
      901 gcttttgacc cgtctgcggg tgaatgcgaa agctgccatc tacatgtgct ccaaaggtca
      961 ttccttcgtc atagcggttg aacatgggcg ggtaaaccat atttggcagc gtcgcactga
     1021 taaatagagg atggcgctgc aaaacatcca gaataattct ctggcagatt cgcgccactt
     1081 ccgattgctc gtcgatttgc cggttgaatt tcaccggcgc cccttggtag cccgccgtgg
     1141 cgcgtccatc cacccaggcc gtgccggcct ggtccaaatg attgcgaaca gcttcgaccg
     1201 cttgcgcgtc aagcacattg ggaatcgtga tcagcatttt gttcttggtt gggttgaacg
     1261 aaggattaca tgcgataggc gaaagtcaac atgacggtgc gtcccgagcc gggtacggcg
     1321 cgaccgccat cggactggat aagcgcgtcg taataattgg cattggtcag gttgaatacg
     1381 ttcagcctga tgtcgtagct tgactggtga taggccagcg ttgcatccgt gcgcgcataa
     1441 cggcccaccc tgaccagatt gttattattg gcaaaacggc tggacacata ggtcacgccg
     1501 ccgccaattt cccaatgtgg cagaaaacgg tacgtcgtcc acagcgatgc ggagtccctg
     1561 ggagtgttgg ccggcgtcat gccttgcgtc cctgcggaaa tggcgtcgac aatggttgca
     1621 ttcatgtgcg tgtagccccc gaagactcgc catttttcgg tgattcggcc cgtggcgctt
     1681 gcgcgtacgc catttacccg gattgtgccg ttggcggtgt aggtattgtc ggcgttttgc
     1741 gagcgcgaat tgtattgagt gatctggaag agggcgctgt caagcgatag atcgccatcg
     1801 agcaaatccc attttccacc aagctcgtac gatttgtttt tctccggagc cagcggctgg
     1861 gaaagccccg ttgtggaagt gagttgttcc agagacgggt tgaacgaagt gctgtacgaa
     1921 acataatagg actgtgcgcc tgtgggttgc caaagtgcgc cagcgcgcca actggcaagg
     1981 tttaccgtct ggttcgccga agccagggtc gtattgcgaa ccgtgttgga cgaattgatt
     2041 gagttggata tagtcgccgc gtaatggtcg taacgcatgc cgcccaccag cttgaattgt
     2101 ggactcaatt caacggtgtc gctgatatac gccgcaatcg tggtcgcgct gcccgcggcg
     2161 agattgcctg gcaggctggg tgcgtttgat ggcgacgagg tatagccggg attcagcagc
     2221 ggcacgcagt caacataacc gctggtagcg ccggcggcgg tcaatggcac gccgttgcat
     2281 gtgccgtttc ggtagtagcc ttgattg
//
LOCUS       DATCXU010000351         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_478419,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000351 DATCXU010000000
VERSION     DATCXU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478419"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..869)
                     /gene="istB"
                     /locus_tag="VIR04_07945"
     CDS             complement(114..869)
                     /gene="istB"
                     /locus_tag="VIR04_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY9460716.1"
                     /translation="MSNVAALPVMLKALRLACIGAHWQKEAKKALAENWVGEQYLAEL
                     CAMELATREDKRLQRYYKESDLPPGKTLAGFDFNAVKGVDAKQVNDLIQNPQWTQRGD
                     NVLLFGASGLGKTHLASSIGHGLIAAGIRVKFMGATHMVQELQRAKERLALADALNKL
                     DKYAVVILDDLGYVKKTDQETSVLFELIAHRYERGSLIITSNQGFQDWDHLFDDSVMT
                     VAAIDRLVHHAMIMTCEGESYRRKTSMKRRATS"
     gene            complement(862..2361)
                     /gene="istA"
                     /locus_tag="VIR04_07950"
     CDS             complement(862..2361)
                     /gene="istA"
                     /locus_tag="VIR04_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY9460717.1"
                     /translation="MSGKYITDQQIKLYMEHRRDSTQAVAAAKAGISERSARRIDSQE
                     LQPGTAARPWRTRADPLIDVWDTIVVPLLERSPSITTIGLFDHLCEHHADRFDPRARR
                     TLERRVLAWRQLRGPDRDVIFLQTHAPGQLGIADFTVVDEPVTVAHNPLHHRLLNYRL
                     VASGWAYAQVVCGGESFSALSDGLQNAFWQSGGVPRELRTDSLSAAYKNRAEHDDFTR
                     RFELLCRHYGIIATRNNRGVAHENGAIESPNNHLKRQLKQALLLRGSCDFTSLQNYNQ
                     FVQQLVARRNRRVVTAFKDEQRQLQPLPAGRSVNYSEHTLRVTRSSTIELRRVVYTVP
                     SRLIGSRVTVRLFDTKLEIWCAQVHTLTLDRVYSPNKNTRVRSVDYRHVIEALVKKPR
                     AFRYSQMRDELLPSHDYQMIWEYVDRTLAADAACQYIVRLLHLAKKTDNEGAIGRYVL
                     GRLATVGLPSMIDCEQRFLERRLAPPRVTIHQHALSDYDLLLKPEAAHV"
BASE COUNT          563 a          805 c          795 g          660 t
ORIGIN      
        1 actgcagttg tcgaacttca ttcaatttgg accttacttc gctcaatatt cagcgtcaac
       61 tatcttgtcc ggtgcgcgtc aattaacttg gccggttgac gccggctaaa taattaactc
      121 gtggccctcc ttttcattga agtttttctg cggtaactct cgccttcgca ggtcataatc
      181 atggcgtggt gaaccaggcg gtcaatggcc gcgacagtca tcacgctgtc gtcaaacagg
      241 tgatcccagt cctggaagcc ttgattcgat gtaataatca ggctaccgcg ctcatagcga
      301 tgggcgatga gttcaaacag aacgctggtt tcctgatctg tttttttgac atagcccagg
      361 tcgtcgagaa tcacaacggc gtatttgtcc agcttattga gcgcgtcagc cagggcaagc
      421 cgctctttgg cgcgctgcag ctcttgcacc atgtgcgtgg cgcccataaa tttgactcgt
      481 atgccggctg cgatcaggcc atgtccgata ctactggcga ggtgcgtttt ccccagaccg
      541 ctggccccga agagcagcac attgtcgccg cgctgggtcc actgcggatt ctgaatcaag
      601 tcattgacct gcttcgcgtc aacgcctttc acggcgttga agtcgaaccc ggctagggtt
      661 tttcccggcg gcagatccga ctccttgtaa tagcgctgca gccgtttgtc ctcgcgcgtg
      721 gccagttcca ttgcgcacag ctcggccagg tattgctcgc ccacccagtt ctcggccaat
      781 gccttcttgg cttctttctg ccagtgcgcg ccgatacacg ccagacgcaa cgctttaagc
      841 atgacgggta acgccgcgac attagacatg agcggcctcg ggtttgagca gcaagtcgta
      901 atccgagagc gcgtgctgat gtatggtgac ccgcggcggc gccaagcgcc tttcgaggaa
      961 gcgctgctcg cagtcgatca tgctgggcag gccgacggtt gccaagcggc cgaggacgta
     1021 gcggccgatg gcgccttcgt tgtccgtctt cttggccaga tgaagcaagc gcacgatgta
     1081 ctggcaggcg gcgtccgccg ccaaggtgcg atcgacatac tcccagatca tttgataatc
     1141 gtggctgggc agcaactcat cgcgcatttg cgagtaccgg aacgctcgcg gttttttaac
     1201 cagcgcctcg atcacgtggc ggtaatccac cgatcgcact cgtgtattct tgttggggct
     1261 atacacccga tccagagtca gcgtgtggac ttgcgcgcac cagatctcca gcttcgtgtc
     1321 gaacaaccgc accgtgaccc ggctgccgat caggcgcgag ggcacggtat aaacgacgcg
     1381 ccttaattca atggtgctgc tgcgggtcac ccgaagggtg tgttcgctgt aattgacgct
     1441 gcgtccagcg ggcaacggtt gtaattgtcg ctgctcgtct ttaaaggcgg tgaccacgcg
     1501 acgattacgc ctggccacga gttgctgcac gaactggttg tagttctgca gcgaggtaaa
     1561 gtcacagctg ccgcgcagca gcaacgcttg ttttaattga cgcttgagat ggttgttggg
     1621 cgattcaatc gcaccgttct catgggccac cccgcggttg ttgcgggtcg cgataatacc
     1681 gtagtgtcgg cagagcaatt cgaaccggcg ggtgaagtcg tcgtgttcgg cgcggttctt
     1741 gtacgcggcg ctcaggctat cggtgcgcag ctccctgggc acgccgccac tttgccagaa
     1801 agcgttctgc agcccgtcgg acagggcgct gaagctttct ccgccgcaca ccacttgggc
     1861 gtaggcccag ccactggcca ccagtcggta attgagcagg cggtgatgca gcgggttatg
     1921 ggcgacagtg acgggctcgt cgaccacagt aaagtcggcg attccgagct gacctggcgc
     1981 atgcgtttga aggaaaatga catcgcgatc gggcccgcgt aattgacgcc aggccaacac
     2041 ccggcgctcc agtgtgcgcc gcgccctggg atcgaatcgg tccgcgtggt gttcgcacaa
     2101 atgatcgaac aggccgatgg tggtgatgga cggcgacctc tccaatagcg ggacgacaat
     2161 ggtgtcccaa acgtcaatta aaggatcggc gcgtgtgcgc cacgggcggg ctgctgtgcc
     2221 cggttgaagt tcctggctgt ctatccgcct tgctgaccgc tctgaaatgc ctgctttggc
     2281 tgccgccacc gcttgtgtac tatctcttcg atgttccata tataacttga tttgttgatc
     2341 tgtaatgtat ttgcccgaca cagcgcgtcc tccgatttgc tgtgtcgaac cctatcagat
     2401 caaccggcca agataattgt cgctggccgg actagttaat tgtcgccgaa cactaactca
     2461 taaattgccg caaagtgatt ccccaacact tccaaccaca tgccaactcc cagccatcga
     2521 agtgtcggac aatgcatttg attgatttaa aagcactcgc tatctgtgcc cggggaagtg
     2581 attttcattg cctgaaaagc cagtaaatac aaattgcccc ggatgcgaga ataagcacta
     2641 tccctgccgt ggcttgcttg ggaatgccta cacggcaaca ggtagcgcac ctcccaaggc
     2701 cgcaccgctt agggcttgga caagtttatt gtctacgtag tcgacattgc tttgtggtgc
     2761 tgcttgggtt ataggttgga gagcagaata accacctccc agtaaagcgc caattcccat
     2821 tcg
//
LOCUS       DATCXU010000352         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_682936,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000352 DATCXU010000000
VERSION     DATCXU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682936"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /gene="pyrC"
                     /locus_tag="VIR04_07955"
     CDS             complement(<1..976)
                     /gene="pyrC"
                     /locus_tag="VIR04_07955"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY9460718.1"
                     /translation="MQRTADTLTIIRPDDWHLHLRDGAMLDTVAGDTARQFGRAIIMP
                     NLKPPVTTTAHALAYRGRIMMALKRAELRAGVGPGAWTDRFTPLMTLYLTDNTTSAEI
                     IRAHGSGVVHAVKYYPAGATTNSDAGVTDLLRRCAAALDAMQRIGMPLLVHGEVTDKD
                     VDLFDREAVFIDRVMIPLRRAFPELKVVFEHVTTQEGADYVRNADGPIGATITAHHLL
                     YNRNALFQGGLRPHWYCLPVLKREKHRLALLQAATDDSGRFFLGTDSAPHRKGLKEHA
                     CGCAGCYTALHAMPLYAQAFDSINKLDRLEAFASINGPAFYGLPVSKEV"
     gene            1094..1366
                     /locus_tag="VIR04_07960"
     CDS             1094..1366
                     /locus_tag="VIR04_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY9460719.1"
                     /translation="MVTTTKPARKPNAAFMKPLTPSQTLAAIIGADAIPRTEVTKKIW
                     EYIKKHDLQDPKNRRNINADDKLRPLFGKEQVSMFELTKLVSGHLK"
     gene            complement(1482..1847)
                     /locus_tag="VIR04_07965"
     CDS             complement(1482..1847)
                     /locus_tag="VIR04_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460720.1"
                     /translation="MTEELLPEGEEGPLMRRLTHAIYGLFALGLISAGFLGVATLAAI
                     VLAYLKRSDAIGTIYAAHFDWVIKTFWWGLLWFVLSAIATMVFIGWITGLIALVWVVY
                     RIIKGWLALLSDRTPAGDL"
BASE COUNT          509 a          570 c          518 g          426 t
ORIGIN      
        1 gcacttcttt actcacgggc aagccataaa atgccggccc attgatactg gcaaatgctt
       61 caagccgatc aagcttgttg atggaatcga acgcctgcgc gtaaagcggc atggcatgca
      121 atgcggtata gcagccggcg cacccacagg catgttcttt caatcccttg cggtgtggtg
      181 cactgtccgt gcctaaaaaa aagcggccgc tgtcgtccgt ggccgcttgc aacagggcca
      241 ggcggtgctt ttcacgtttt aaaacgggca aacaatacca atgcggccgc agaccgccct
      301 gaaacagggc attgcggtta tacagcaaat ggtgtgcggt gatggtggcg cctatggggc
      361 cgtcggcatt tcgcacataa tccgcccctt cttgtgtggt cacatgctcg aataccacct
      421 tcagctcggg aaaggcccga cgcaacggaa tcatgacacg gtctataaat accgcttcgc
      481 ggtcgaacag atcgacatcc ttgtcggtta cttcgccatg caccagtaat ggcatgccga
      541 tcctttgcat ggcgtcaagc gccgccgcac agcgccgcag caagtcagtc accccggcgt
      601 cggagttcgt tgtggcgcct gcagggtaat atttaacggc gtgcacgaca cccgagccgt
      661 gcgccctgat gatttcggcg cttgtcgtat tgtcggtcag gtataacgtc attaatggcg
      721 taaaacgatc tgtccacgct ccgggaccga ctccggcccg gagctcggcg cgcttcagtg
      781 ccatcattat tcggccccga taagccagtg cgtgcgcggt tgtcgtcact ggcggcttca
      841 agttgggcat gataatggcg cgcccgaatt ggcgcgccgt gtcgcctgca acggtatcca
      901 gcatcgcacc atcgcgcaga tgaagatgcc agtcatcggg acgaatgatc gttaaagtat
      961 cagcggtgcg ttgcatttga ttcaatttat ccgtcttttt gaaacattcc acataaatca
     1021 ctttgtcttt cgacgtatct cacgttatgc tcaggaaagc atttttttca ccaatagcta
     1081 ggagcattac cctatggtaa caaccaccaa gcccgcacgt aaacccaacg cggcattcat
     1141 gaaacctctt acgcctagcc agacattagc cgccattatc ggcgcagacg cgatcccccg
     1201 caccgaagtc accaagaaaa tctgggaata tataaaaaaa cacgacttgc aggatccgaa
     1261 gaaccgccgc aatatcaatg ccgacgacaa attgcgcccg ctatttggca aagaacaggt
     1321 ttccatgttc gaacttacga aactggtcag cggacacctt aaatagaaaa agcgggtctg
     1381 cgtattaatc aggcgagcta tgtctgattc ttcttaagtt tttatataaa aaaatgctgc
     1441 caggatccgg gcagcatttt tgtatggtgt ggcaaaaacg atcagaggtc gcctgccgga
     1501 gttctgtcgg acagcaatgc aagccatcct ttaataatcc gatacaccac ccacaccagg
     1561 gcaatcagcc cggttatcca tccaataaaa accatggtgg caatagcgct gagaacgaac
     1621 cagagcaagc cccaccagaa tgttttgatg acccagtcaa agtgagccgc gtaaatcgta
     1681 ccgatcgcgt cgctgcgttt caaataagcc aaaacgatcg ccgcgagggt cgccacccca
     1741 agaaagccgg cgctaatcaa gcccaatgcg aaaagcccat aaatagcatg cgtcaagcgg
     1801 cgcatgagcg ggccttcttc accttccggc aacagttcct cggtcatgga aacctccgta
     1861 aatgaattaa agtacaaaga cacagccgct attctacccg acccgtagcc agctatgaca
     1921 cggctcaggc ggcgcggcct cggagtgtgg gggccacaac agcaacgccc ccgggcggtg
     1981 aggcgcgggg gcgttgggga ctgaataaga gcttgacgat gac
//
LOCUS       DATCXU010000353         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_722157,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000353 DATCXU010000000
VERSION     DATCXU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722157"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="hisD"
                     /locus_tag="VIR04_07970"
     CDS             <1..738
                     /gene="hisD"
                     /locus_tag="VIR04_07970"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY9460721.1"
                     /translation="QAIGALAYGTESLAAVDKITGPGNAYVAAAKRRVFGAVGIDMIA
                     GPSEILVICDGSTPADWIAMDLFSQAEHDELAQAILLCPDAAFLDEVEAAMKRLLPSL
                     PRADIIKASLAQRGALILVRDLHEACAIANDIAPEHLEISTENPRQWTKKIRHAGAIF
                     LGRYSSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQKRSSLIQISQAGAQTLGRVA
                     ARLANGEGLQAHAASAQYRLDPALSKK"
     gene            857..1444
                     /gene="hisB"
                     /locus_tag="VIR04_07975"
     CDS             857..1444
                     /gene="hisB"
                     /locus_tag="VIR04_07975"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY9460722.1"
                     /translation="MRTADITRNTNETRIRVAINLDGAGRQTINTGVPFLDHMLDQIA
                     RHGLIDLDIHCEGDTQIDDHHTVEDVGITLGQAFAAALGNKAGICRYGHSYVPLDEAL
                     SRVVIDFSGRPGLEYHIPFTRSHIGQFDVDLTREFFQGFANHALATLHIDNLRGTNSH
                     HQCETVFKAFGRALRMAVELDPRSAGVVPSTKGVL"
     gene            1465..2121
                     /gene="hisH"
                     /locus_tag="VIR04_07980"
     CDS             1465..2121
                     /gene="hisH"
                     /locus_tag="VIR04_07980"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY9460723.1"
                     /translation="MNTIAIVDYGMGNFYSVARALRAAAPEADVKICHTAQAIDDASR
                     VVFPGQGAMPDCMRTLNESGLRDAVTRAARAKPLLGVCVGEQMLFTRSEEGPTSCLDI
                     FPGKVKRFAGAPYASAALCTNPHNELLKVPHMGWSRVRQTKPHALWDGIPDNSHFYFV
                     HSYYAEPDNRALIAGQTHYGVSFTCAIAQANIFAVQFHPEKSAAYGLRLYRNFVHWSP
                     "
     gene            2164..2907
                     /gene="hisA"
                     /locus_tag="VIR04_07985"
     CDS             2164..2907
                     /gene="hisA"
                     /locus_tag="VIR04_07985"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY9460724.1"
                     /translation="MLLIPAIDLQDGRCVRLRQGDLDDATIFSDDPAAMAAQWLEQGA
                     RRLHLVDLNGAVAGKPKNEAAIKSIIKAIDGEIPVQIGGGIRNLDTIERYLDSGISYV
                     IIGTAAVKDPGFLRDACSAFPNNIIVGLDARDGKVATDGWSKLTRHDVLDLARKFEEY
                     GCEAIIYTDIGRDGMLTGVNIEATVALAQHVTVPIFASGGVTDLEDIKALCAVEDQGI
                     QGAILGRSLYEGTLDFAAAQSLADEFGAP"
     gene            2904..>3252
                     /locus_tag="VIR04_07990"
     CDS             2904..>3252
                     /locus_tag="VIR04_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY9460725.1"
                     /translation="MTDSVTPTSDTLTRRIIPCLDVTAGRVVKGINFVNLIDAGDPVE
                     IARRYDEQGADELTFLDITATSDGRDLILPIIEAVAAQVFIPLTVGGGVRQVADIQRL
                     LNAGADKVSINSAA"
BASE COUNT          692 a         1014 c          885 g          661 t
ORIGIN      
        1 caagccatcg gcgccttggc gtacggcact gaaagcctgg ccgcggtgga caaaattact
       61 ggccccggaa acgcctacgt ggccgccgca aaacgccgtg tattcggcgc cgtcggaatc
      121 gacatgattg ccggcccaag cgaaattctg gtcatctgcg acggttccac gccggccgac
      181 tggatcgcca tggacctgtt ctcacaagcc gaacacgacg aactcgcgca agccattttg
      241 ctttgcccgg atgccgcttt tcttgatgaa gtagaggccg ccatgaagcg gctgttgccc
      301 agcctgccgc gcgccgacat catcaaggcc agcctggccc agcggggcgc gctcatactt
      361 gtgcgggatt tgcacgaagc gtgcgctatt gccaacgaca tcgcgcccga acacctggaa
      421 atctccactg aaaatccacg tcagtggacg aagaaaattc gtcatgcggg cgccatcttc
      481 ctgggtcgct acagttccga ggcgctcggg gattactgcg ccggacccaa tcatgtgctg
      541 cccacttcac gcaccgcgcg attttcatcg ccgctgggcg tttacgattt ccagaagcgg
      601 tccagcctga tccagatctc gcaagccgga gcacagacgc tggggcgtgt ggcggcccgt
      661 ctggcgaacg gagaaggcct tcaggcccac gcggcaagcg cccaataccg ccttgacccc
      721 gccctgtcaa agaaataggc cgagcgacac ggttacacca agtggcttag ccaagccacc
      781 gtttttctgt tagtgtctta agtctgcggg ctttgcaatc gccgcaagct ttaacccgga
      841 ttctaaagtt cccaccatgc gcactgccga tatcacccga aataccaacg aaacacgcat
      901 acgagtcgcc atcaatcttg atggtgcagg ccgtcaaacg atcaataccg gcgtgccttt
      961 tctggatcac atgctggacc agatagcgcg ccatggacta atagaccttg acatccactg
     1021 cgaaggcgat acgcagatcg acgaccacca caccgttgaa gacgtcggca tcacgcttgg
     1081 ccaggccttt gcggccgcct tgggcaataa agcgggcatt tgccgctatg ggcactcgta
     1141 cgtgccgctg gacgaagcgc tatcgcgcgt ggtcatcgac ttttccgggc gcccgggact
     1201 ggaataccac attcccttta cccggtcgca catcggccaa tttgacgtcg atttaacgcg
     1261 cgaatttttt cagggcttcg ccaaccatgc tctggccacc ctgcatatcg acaacttgcg
     1321 gggcacgaac tcgcatcatc aatgcgaaac cgtgttcaaa gcctttggac gcgctttgcg
     1381 tatggctgtc gagctcgatc cacgcagcgc aggcgtagtg ccttcaacca agggcgttct
     1441 ttaaccttca atctcagacc gaccgtgaat actatcgcca ttgttgatta cggcatgggc
     1501 aatttctatt cggttgcgcg cgccttgcgc gccgccgccc cggaagctga tgtcaagatc
     1561 tgccacacag cacaggccat cgatgacgca tcgcgcgtcg tattcccggg ccagggagcc
     1621 atgcccgact gcatgcgcac tctgaatgaa tcgggtctgc gcgatgccgt gactcgcgcg
     1681 gcgcgcgcca aacccctgct gggcgtgtgc gtcggcgagc aaatgctgtt cacacgaagc
     1741 gaagaaggtc ctacttcctg tctggatatt ttccccggca aggtcaaacg cttcgctggc
     1801 gcgccctacg cttccgccgc tctttgcaca aatccccaca acgaactgtt aaaagtgccc
     1861 catatgggat ggagccgcgt acgacagaca aaaccgcatg cgctttggga cggcatcccc
     1921 gataacagtc atttctactt cgtgcacagt tactacgccg agcccgataa tcgggcgcta
     1981 atcgcgggcc agacccatta cggggtaagc tttacgtgcg ccattgcaca ggctaatatt
     2041 ttcgcagtac agtttcatcc cgagaaaagc gcggcctacg gcttgcgttt gtatcgcaac
     2101 ttcgtccact ggagtccctg actcttatcc ttatctactt ttaccgacaa ccggctaccg
     2161 atcatgctcc tcattcccgc catcgactta caagacgggc gctgcgtacg actgcgccaa
     2221 ggtgatctag acgatgcaac cattttttcc gacgaccccg cggccatggc ggcgcagtgg
     2281 ctcgaacaag gcgcccggcg tttgcatctg gtggatctga atggcgcagt tgccggaaaa
     2341 ccgaaaaacg aagcggcaat caagtcgatc atcaaggcga ttgatggcga aataccggtg
     2401 caaatcgggg gaggcattcg caacctggac accattgaac gctatctgga cagcggcata
     2461 tcttatgtca ttatcggaac tgcggcggtc aaagatccgg gatttctgcg cgacgcgtgc
     2521 agcgcgtttc cgaacaatat tattgtcggc ctcgacgccc gcgacggaaa agtggccacc
     2581 gacggctgga gcaaacttac tcgccacgac gtgctcgatt tggcgcgcaa gttcgaggag
     2641 tacggctgcg aggcaatcat ttataccgac atcggccgcg acggcatgct gaccggcgtc
     2701 aacatcgagg ccaccgtggc cttggcgcag catgtgaccg tacctatctt cgcctcgggc
     2761 ggggtaaccg accttgaaga catcaaagcc ctttgcgccg ttgaagacca gggcatccag
     2821 ggagccatcc ttggccgcag cctttatgaa ggcacccttg attttgccgc cgcccaaagc
     2881 ctggccgatg aatttggcgc cccatgacag attccgtaac gcccacctcc gacaccttga
     2941 cgcgacgcat tatcccttgt ctggacgtta cagcgggtcg tgtcgtaaaa ggcattaatt
     3001 ttgtgaacct gatcgatgcg ggcgatccgg tcgaaatcgc ccggcgttac gacgaacagg
     3061 gggccgacga actcactttt ctcgatatca ccgccaccag cgacggccga gatctgattc
     3121 tccctatcat cgaggcggtg gcggcccaag tgtttattcc gctgaccgtc ggcggcggcg
     3181 ttcgccaggt cgccgacata cagcggctgc tcaatgcggg cgccgacaag gtgtccatca
     3241 acagcgccgc cg
//
LOCUS       DATCXU010000354         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_426158,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000354 DATCXU010000000
VERSION     DATCXU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426158"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..996
                     /gene="dapF"
                     /locus_tag="VIR04_07995"
     CDS             85..996
                     /gene="dapF"
                     /locus_tag="VIR04_07995"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY9460726.1"
                     /translation="MGDNSLMIWNFSKMHGAGNDFVVLDGVRQNIEMTPERARALADR
                     HFGIGADQILLVQTPNHPDADFRYRIFNADGGEVEHCGNGARCFVNFVHEQGLSGRNP
                     LRAEICSGLITLTETPSGEVSVDMGETSFDPEDVGFDTRGLTSTTQHDDTLWTLPLPG
                     RQAVQISLVAISNPHAVQVVDDVFTAPVAEVGPLIEGHPRFARRVNAGFMQIVDRNTI
                     KLRVYERGAGETLACGTGACAAAIAGIRRGLLDSPVVVQTRGGTLTIARDGNRLTMSG
                     PVATVFSGNIDIDALTDTFNPKDPLRS"
     gene            993..1685
                     /locus_tag="VIR04_08000"
     CDS             993..1685
                     /locus_tag="VIR04_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY9460727.1"
                     /translation="MSTDILSAEDVAQFLTENREFFQDHAELFANLHVPHPHETRAIS
                     LGERQIMILRARARDLEWKLSGLVDNASGNEKISKTVNAWCCRMLAETDATKIPGHIV
                     RSLGDLFDLPAIALRLWDLPGLVDSEFTQDVNESIKQYARQLTTPYCGPLKDQEAAAW
                     LGSSPASLAIVALRINNADAPFGLLVLGSDDIDRFTPDMGTAFLSTIRDLSSASLSRL
                     KKAPQAEPADQA"
     gene            1692..2657
                     /gene="xerC"
                     /locus_tag="VIR04_08005"
     CDS             1692..2657
                     /gene="xerC"
                     /locus_tag="VIR04_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY9460728.1"
                     /translation="MGQAPVPALPVPMEQWLNHLKVNRRYSSHTLSGYRQDLRHLVPP
                     ESDVALERLTESHIRRTLARLHGQGLKPTSLARALAAWRGFFQWWAPMAGLDRNPVTG
                     VRAPKAPRGLPKALSVEQTQALLDRAGLPQPKTPVEWRDQAMFEMLYSSGLRLAELVS
                     LDWRYTRAGSYESTSWVQLTEHEATVKGKGNKTRTVPVGSKAILAVNNWLELREQFLQ
                     PDATTDSQAALFLGTRGKRISPRVVELQLNKLVTLAGLPVHVHPHSLRHSFASHVLQS
                     AQDLRAVQELLGHANISTTQIYTRLDFQHLAKVYDQAHPRANRKR"
     gene            complement(2659..>3233)
                     /locus_tag="VIR04_08010"
     CDS             complement(2659..>3233)
                     /locus_tag="VIR04_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY9460729.1"
                     /translation="HGAGSGNAGHPANIDPHAWQGLANGEIYAKNIAAGLAKADPVHA
                     SDYQTRAAAYIERMKALDKEIKIMLSQVPGERRRVVTSHDAFGYFGQAYGIQFISAAG
                     VSSEAEPSAKDIAGIIDQVRKEHVPAVFIENITSPKLVEQIARETGAKVGGTLYSDAL
                     AQPGKPAGTYLGMFKWNARQLIDALRPAAR"
BASE COUNT          661 a         1050 c          918 g          604 t
ORIGIN      
        1 aggcggatcc ctccggcgag cccggcatgg cctgccccca ccccgcgaca gcgcaccagt
       61 gaatactctg caaacaaacc gcgattgggc gataattccc tgatgatctg gaacttctca
      121 aaaatgcacg gagccggcaa cgacttcgtc gtcctcgacg gcgtgcgcca aaacattgaa
      181 atgacgcccg aacgcgctcg cgcgctggcg gatcgccatt ttggcatcgg cgccgaccaa
      241 attctgctcg tccagacccc gaaccatccc gacgccgact tccggtaccg catattcaac
      301 gccgatggcg gcgaagtcga acactgcggc aacggcgccc gttgctttgt gaatttcgtc
      361 cacgagcagg gcttgtccgg ccgcaatccc ttgcgcgccg aaatctgtag cgggcttatc
      421 acgctgaccg agaccccctc gggcgaggtt tcggtcgaca tgggcgaaac ctctttcgat
      481 ccggaagacg ttggtttcga tacccgcggc ctgacctcca caacgcaaca cgacgacacc
      541 ctctggacac tgcccttgcc cggacgccag gccgttcaga tatcgctggt cgccatctca
      601 aatccgcatg ctgtccaggt ggtggacgac gtctttaccg cacccgtggc tgaagtcggc
      661 ccgcttatcg aagggcaccc gcgtttcgcg cgacgcgtta atgcagggtt catgcaaatc
      721 gtcgaccgca acaccatcaa attgcgcgtc tacgaacgcg gcgctggcga aaccctggcc
      781 tgcggcactg gcgcctgtgc ggccgcgata gcgggaatcc gccggggctt gctcgacagc
      841 ccggtcgtcg tgcaaacccg cggcgggacc ctgaccattg cccgcgatgg caacaggctg
      901 accatgagcg gcccggttgc aaccgttttt tcaggaaaca tcgacattga tgcgctcacc
      961 gacacgttca accccaagga cccgctaaga tcatgagtac tgatatcctg tctgccgaag
     1021 acgttgccca atttctcacc gagaaccgcg aattttttca ggatcacgcc gaactgtttg
     1081 ccaatctgca tgttccgcac ccgcacgaaa cgcgggcgat atcgctgggt gaacgccaaa
     1141 taatgatctt gcgcgcgcgc gcccgcgatc tggagtggaa gctttcgggc ctggtcgata
     1201 atgcctcggg caatgaaaag atcagcaaaa ccgtgaacgc atggtgttgc cgcatgcttg
     1261 ccgaaaccga tgccacaaaa attccggggc atatcgtgcg cagtctgggt gacctgttcg
     1321 atctgcccgc cattgcactg cggctgtggg acctgccggg tctggttgac agcgaattca
     1381 cacaagacgt gaacgaatca atcaagcagt atgcacggca actgactacg ccctattgcg
     1441 gcccgcttaa agatcaggaa gcggctgcgt ggctaggctc cagccccgca tcgctggcca
     1501 tcgtcgcgct caggatcaac aacgccgatg ctccgttcgg tctgctcgtt ctgggctcgg
     1561 atgacatcga ccgctttacc cccgacatgg gcacagcgtt cctgagcacg atccgcgatt
     1621 tatccagcgc cagcctaagc cgcctgaaaa aagcgccaca ggccgaaccc gccgatcagg
     1681 cctgacccgc catgggacag gcgcccgtgc ccgccttgcc ggttccgatg gagcaatggc
     1741 tcaatcatct gaaagtcaac cggcgctatt ccagccacac actgtccgga taccgccagg
     1801 atttgcggca tctggtcccg cccgagtccg atgtcgcgct ggagcgcctg accgagagcc
     1861 atatccgacg cacactggcc cggctgcacg gacaaggcct gaaaccaact agccttgcgc
     1921 gcgcgctggc agcatggcgc ggattttttc agtggtgggc gcccatggcc gggctggacc
     1981 gcaaccccgt cacaggcgtg cgcgccccca aggcgccacg cggcctgccc aaagccctgt
     2041 cagtcgaaca aacacaggcg ttgctggaca gagcgggcct gccgcaaccc aaaacgcccg
     2101 ttgaatggcg cgaccaggcc atgttcgaaa tgctgtattc cagcggcttg cggctggctg
     2161 agctggtcag cctggactgg cgctacacac gcgcgggatc ctatgaatcg acaagctggg
     2221 ttcaactgac cgagcacgag gcgaccgtca aggggaaagg gaacaagacg cgcaccgtgc
     2281 cggtcggatc caaggccatc ctggcggtta acaactggct tgaattgcgc gagcagtttc
     2341 tgcagcccga tgccacgacg gacagccagg ccgcgttatt tttgggaacc cgcggaaaac
     2401 gcatttcgcc acgggttgtc gaactgcaac tgaacaagct ggttacgctg gccgggctgc
     2461 cggtgcatgt gcatccgcac agtttgcgcc atagtttcgc cagccatgta ctgcagtcgg
     2521 cgcaggattt acgggcggtg caagagctgc tgggccacgc gaatatttcg accacgcaaa
     2581 tttacacccg gctggatttt caacatctgg cgaaggtata cgatcaggcg cacccgcggg
     2641 caaatcgcaa acggtaattc aacgcgccgc cggccgcaac gcatcaatca actggcgtgc
     2701 gttccacttg aacatgccca gatacgtacc tgccggcttt cccggctgag ccagcgcatc
     2761 ggaatacagc gtgccgccca ctttcgcgcc ggtttcacgc gcaatttgct ccaccagctt
     2821 cgggctggtg atgttctcga taaagaccgc cggaacgtgc tcttttctga cctggtcaat
     2881 gattcccgca atatccttgg ccgacggctc ggcctcgctg gacacgccgg cagccgaaat
     2941 gaactgtatg ccatacgcct gcccgaaata gccgaacgcg tcgtgcgagg tcacaaccct
     3001 gcgccgctcg ccgggaacct gggacaacat gatcttgatt tccttgtcca gcgccttcat
     3061 gcgttcaata taggcggcgg cgcgcgtctg atagtcagac gcgtgaaccg ggtccgcctt
     3121 tgccaggccg gccgctatat tctttgcgta aatctcgcca ttcgccagac cttgccaggc
     3181 atgcgggtca atattggcgg gatggccggc attgcccgat ccggcgccat gct
//
LOCUS       DATCXU010000355         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_236495,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000355 DATCXU010000000
VERSION     DATCXU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236495"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIR04_08015"
     CDS             complement(<1..341)
                     /locus_tag="VIR04_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9460730.1"
                     /translation="MDASLTASIQQQARLMDIASKLAKIGISGALFKLYAAAIELGEA
                     PISAVATRAGIVRTSAYDALGRLEQEGLVSLQTRKGRRYVIAQDPTVLLERLEGRRQM
                     LDELMPQLRSM"
     gene            563..1600
                     /locus_tag="VIR04_08020"
     CDS             563..1600
                     /locus_tag="VIR04_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY9460731.1"
                     /translation="MLNLPTIKVAAVQASPVFLDTNATIDKACALIAQAAERGASLVA
                     FPEVFVPGYPYWNWFMSPVEGSPWFQKLCMAAIEIPGPEINRVAQAAKKFGVHVVIGV
                     NERSKAGVATLYNTLVFIGPDGAILGRHRKLVPTWAEKLTWANGDGSSLRVYGTEIGK
                     LGGLACGENTNTLARFSLLAQGELIHVASYISLPVAPPDYDMSEAIKVRSMAHSFEGK
                     VFTVVSCSTISEEIIAAFEATHPEIRENFQRKNSAFSGVIGPDGRVIGQPLIDQEGIV
                     YADIDLSRCIQPRQMHDITGHYNRFDIFDLRVNRRPQQSAQYFDESVSNNDFSDARDS
                     ANSFDEAGASR"
     gene            1597..2352
                     /locus_tag="VIR04_08025"
     CDS             1597..2352
                     /locus_tag="VIR04_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HEY9460732.1"
                     /translation="MSKRLYRVGQIVPSSNTTMETEIPAMLRARETTFPERFTFHSSR
                     MRMKKVVKEELELMDHDSDRCAIELSDARVDVLGYACLVAIMSMGKGYHRVSEKRLEQ
                     RTVENGAPAPVVTSAGALVDGLHAMGAKTVSLVCPYMKPLTRLVVDYVESEGINVKDY
                     VALEIPDNLQVAAQDPACLIDIYKRLDRANVDALVLSACVQMQSLPSIAKVEQESGIP
                     TISASVATTWQMLRRLGLPTQISGCGDLLSGKY"
     gene            2393..>3420
                     /locus_tag="VIR04_08030"
     CDS             2393..>3420
                     /locus_tag="VIR04_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460733.1"
                     /translation="MDDHKSSKEGAAVGMDRRSVLKMGVGAAAAGALLPGTVLAADKP
                     PIGNYPAGVSGDSVFVGLTLDLTGPYSAEGADEQKGYELAIEQLNAGAPEIRKISPLT
                     KKGVLGKTVKYGVADSETKPNSAVQAATRFIHENKAMMVSGSVSSAVAIALQKVCDRE
                     KTLYLVAIGGSNEINGKDCQRYGFRLWYFVYSVAKAISPILSKSLGKKRKAIYLVPDY
                     TYGHTTYQSMKEFTEKEGWTTVGQQVHPLGAKDYSSYLINIAHSDADTLIIIDYGADA
                     ANSIKQAKQFGILDKMTLVVPVLSPFLGEELGPDLFQGVYGAEAFWWTLEDRNPIAKD
                     FVTAFEKK"
BASE COUNT          824 a          852 c          937 g          807 t
ORIGIN      
        1 tacatggaac gcaattgcgg catcaactcg tccagcattt gacgtcggcc ttcaaggcgc
       61 tcaagcaaaa cagtcggatc ctgcgcgata acgtagcggc gtcctttgcg cgtctgcaag
      121 ctaaccagac cttcctgctc cagccggcct aacgcgtcat aggccgatgt ccggacgatt
      181 ccggcgcggg tggcgacagc ggaaatgggg gcttcgccaa gttcgatcgc ggccgcataa
      241 agtttgaaca aggcgcccga tattccaatt ttcgcgagtt tgcttgcaat gtccataagg
      301 cgtgcttgtt gctgaatcga agcagtaaga gaggcgtcca ttctagcgct tgttgcagac
      361 agttcattgt gttcatttta ttcgacagtt aactataaga cgcctatatt tttgccgaaa
      421 attatggaat attgtcttct aaaacataaa aacagccgaa aaacctaggg tttaccatag
      481 tttttttgcc atatatcaga cattaaacta aaaaccattg tgttagtttt cttcgacact
      541 tatgcctttc agggactttt ccatgctgaa tttaccgact atcaaggtcg cggcggtgca
      601 ggcatcgccg gtgtttctcg ataccaatgc aaccatcgac aaagcgtgcg cacttattgc
      661 tcaagctgcg gaacggggcg cgtccctggt cgctttcccg gaagttttcg ttcccggcta
      721 tccttattgg aattggttca tgtcgccggt cgaaggcagt ccatggtttc agaaactgtg
      781 catggcagcc atcgagattc cggggccgga aataaatcgg gtagcccagg cggcgaaaaa
      841 attcggcgtg catgtggtta tcggcgtcaa cgaacgcagc aaggcaggcg tggccacgct
      901 ttacaacacg cttgtattta ttgggcccga tggcgcaatt ctggggcggc atcgcaaact
      961 ggtgcccaca tgggcggaaa aacttacttg ggcgaacggc gatggctctt cgctacgtgt
     1021 ctatggcacc gaaattggca agctgggtgg cctggcctgc ggtgagaaca ccaacacgct
     1081 tgcccgattc agccttttgg cgcaaggtga attaatccac gtcgccagtt atatttcctt
     1141 gcccgtcgcg ccgcccgact atgacatgtc cgaagcgatc aaagtgaggt cgatggcgca
     1201 ttcctttgaa ggcaaggtct tcacggttgt gtcgtgcagc acgatttccg aagagattat
     1261 tgccgccttc gaggccaccc atcccgagat acgtgaaaat tttcaacgaa aaaatagcgc
     1321 attctctggt gttatcggac ccgatgggcg tgtgattggg cagccgctga ttgatcaaga
     1381 aggcatcgtc tatgccgata ttgacttgtc gcgttgcata cagccgcggc aaatgcacga
     1441 tatcaccggg cattacaacc gttttgatat cttcgatttg cgggtcaacc ggcggccgca
     1501 acagtcggct caatactttg acgaaagcgt atcgaacaat gatttttccg atgctcgcga
     1561 ttcagccaac tcattcgatg aagccggggc ttcccgatga gcaaacgttt atacagagtg
     1621 ggtcaaatag ttccttcttc caacacaacg atggaaaccg aaattccggc catgctgcgc
     1681 gctcgggaaa caacgttccc cgagcgtttt acttttcatt cgagccggat gcgcatgaaa
     1741 aaggtggtaa aggaagaact cgaactgatg gatcacgaca gcgaccgatg cgcgatcgaa
     1801 ttatcggacg cgcgcgttga tgtattgggc tacgcctgcc tggtcgcgat catgagcatg
     1861 ggcaaaggtt atcaccgcgt ttccgaaaag cggctggagc aacggactgt ggaaaatggc
     1921 gcacccgctc cggtggtgac cagcgctggg gcgttggtcg atggactgca cgcaatggga
     1981 gcgaaaacgg tgtcgctggt gtgcccttat atgaaaccac taacgcgttt ggtcgtggac
     2041 tacgtcgaaa gcgagggaat caacgttaaa gactatgtcg ctttggaaat tcccgacaat
     2101 ctgcaggttg cggcgcaaga tccagcatgt ctgatcgata tttataaacg gcttgatcgc
     2161 gccaatgtgg atgcgctcgt tttgtcggca tgcgttcaaa tgcaatcgct gccatcgatt
     2221 gccaaagtag agcaagaatc gggcattccc actatatccg cgtccgtggc caccacttgg
     2281 caaatgctgc ggcgcctggg cctgcccact caaatttcgg gatgtggcga tttgttgtca
     2341 ggcaagtatt aatcatcaat cggggtttgt tgcacataca aggagttcaa ggatggacga
     2401 tcacaaaagt agcaaagagg gtgcggcggt cggaatggat cgccgatcag tgctgaaaat
     2461 gggtgttgga gccgcagccg ccggagcgtt gttgccagga accgttctgg ccgccgataa
     2521 gccgccgatc ggcaattatc cggctggggt tagcggcgat tcggtatttg ttggccttac
     2581 actggatttg accgggccct attcggccga aggcgccgat gagcaaaagg gctatgaact
     2641 ggctattgag caattaaatg ccggcgctcc ggagataaga aaaatttctc cgctgacaaa
     2701 aaaaggagtg ttgggcaaaa ccgttaagta cggcgttgcc gattccgaga ccaagcccaa
     2761 ttcggccgtg caggccgcga ctcgttttat tcacgagaat aaagcgatga tggtttcggg
     2821 cagcgtcagc agtgcggtgg cgattgcgct gcagaaagta tgtgaccgcg aaaaaacgct
     2881 gtacctggta gctattggcg gatccaatga aatcaatggc aaggactgtc agcgttacgg
     2941 tttccgccta tggtattttg tttactcggt cgcaaaggcg atttcaccga tattgtccaa
     3001 atcattgggc aaaaaacgca aggcgatcta cctggtgccg gattacacct atggccatac
     3061 cacttatcag tcaatgaagg aattcacaga aaaagaaggc tggacgacag tgggtcagca
     3121 ggtgcatccc ctgggcgcca aagactatag ctcctacctt atcaatattg cgcatagcga
     3181 cgccgatacc ttgatcatca tcgactacgg cgctgacgcg gccaactcga tcaagcaggc
     3241 gaaacagttc ggcattcttg acaaaatgac gctggttgtc cctgtcttgt ctccgttcct
     3301 tggcgaagaa ctcggccccg atttgttcca gggggtatac ggcgccgagg ctttttggtg
     3361 gacactggaa gaccggaacc ctattgccaa agactttgtc accgccttcg agaagaaatt
//
LOCUS       DATCXU010000356         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_426340,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000356 DATCXU010000000
VERSION     DATCXU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426340"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1436
                     /locus_tag="VIR04_08035"
     CDS             90..1436
                     /locus_tag="VIR04_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460734.1"
                     /translation="MNRSAGVNCSARGQQGQALVLGLVLTGVVVIVFVRYFGVGQVVA
                     AKARQLHSLDAAAYSGALIQARALNMLAYLNRSQIGHQVAMAHLVTLGSWAAFGANQA
                     RQASVGNPPAYLIAMLFGPDQGAAYAAARAAAGLDALAQADGPLAHAYAGHDSVVRDL
                     FTTVQDSIVASLPDARWAAMQAVLEQNYSASESGADFDLAVSHDNWPGYVQRYSSQHQ
                     LRPFIQSVAQLYDFLSPRNHTASNPWAVEARCPHLRHQLRRRGSTTLDPDGRWQSADT
                     ESFHALRSNRWIGCHYREYPMGWGWIPSAPGQDIGSGHVSDPPKDFAAQDFWRWVKQA
                     TDWDIVTGDANPLANSRAVAGRQRWRGGALPDYFDVGADAAGKALRFTVELKHAGPEG
                     LTITTRSAAETFFQRPEPRTDRRLESSNLFHSYWQARLASPNDLADGSMTDAKGFP"
     gene            1433..2293
                     /locus_tag="VIR04_08040"
     CDS             1433..2293
                     /locus_tag="VIR04_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460735.1"
                     /translation="MKPARSMQPHLRLGLGLFAARRGVSRRSPLNAFSVCRFGMDRHS
                     KARLLACRSRSIHTIHARQAGQALVEGIVILLALLSLWVAISWLGRFQDMALQASHAS
                     RFAAFSLTRNPIAPPVLQGRTDYFSGPDHQWMDRRGQSALSKDRSEVTFSVGRQPVLQ
                     SGAQPGGAASRAQELRQGWRLEDTGILKAYAIVSSSHYPALARHTALLTGAGHASDDA
                     DNQHRLAESPAAWSDSANPSYELGRRIESRVSALDRAWGRSQPHFDWLGPWAGYVPAH
                     HLGEFQGDDK"
     gene            2290..2970
                     /locus_tag="VIR04_08045"
     CDS             2290..2970
                     /locus_tag="VIR04_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460736.1"
                     /translation="MIRMLVCSLLMVTAYLGVPQQAIGSISAQFATLESEPLVWSERQ
                     EMRLFGVPVFQRSFSSMESKQNVAEILAAHTDMFQRAVAFRNKTVLSGAKTGWHWVAE
                     IDSTDFGARGSVSALRIGGPELNHAVAIQSKPGFSWLPQQAKRRFSQRTVARDRQVLD
                     EVYSTALRPEALLGYLQKQLRLRGWVDEPGLAWLPNMSVWRRKASELTLISSASRDGS
                     AVFIHYLE"
BASE COUNT          561 a          872 c          928 g          696 t
ORIGIN      
        1 cgcatggcgc aatcagccgc cgaggccgct gcggcgcagg cagccgctaa aacattgaag
       61 tcattcaccg gcaacgccca aggtgttcaa tgaatcggtc tgccggcgtc aattgctcag
      121 ctcgcggcca gcagggccag gcattggtat tgggtttggt gcttacaggt gtcgtggtta
      181 tcgtctttgt gcgttacttc ggggtcggcc aggtggtggc cgcgaaagcg cggcaattgc
      241 attcgcttga tgcggcggca tacagcgggg cccttatcca ggcgcgtgcg ctgaatatgc
      301 tggcgtactt gaaccgatcc cagattggcc accaggtagc tatggcgcat cttgtcacgc
      361 tggggtcgtg ggcggcattt ggcgcaaacc aggcccgcca ggccagcgtt ggcaacccgc
      421 ccgcctactt gatcgccatg ctctttggtc ccgatcaggg tgcggcatac gcggcggcgc
      481 gcgcagcggc gggcctagac gccctggcgc aagcggacgg tccattggcc catgcttatg
      541 cggggcacga tagcgtcgtc cgggatctat ttacgaccgt gcaagatagt attgtcgcca
      601 gcttgccgga cgctcgctgg gcagccatgc aggctgtgct ggaacaaaac tattcggcct
      661 ccgaatctgg cgcagacttc gacttggctg tcagccatga taactggccc ggttatgtgc
      721 agcgttactc ttctcagcac caattgcgcc ccttcattca gtctgtggca caactttacg
      781 attttctgtc gccgcgcaat catacggcgt ccaatccctg ggccgtcgaa gcgcgttgtc
      841 ctcatttgcg tcatcagttg cggcggcgtg gaagcaccac gctcgatcct gatggacgct
      901 ggcagtcggc ggacactgaa tcatttcatg cactaaggtc taaccgctgg attggttgtc
      961 attaccgtga ataccccatg ggatggggtt ggatccccag tgcgccgggc caggatatcg
     1021 gatccggcca cgtttccgat ccgcccaaag atttcgccgc gcaagatttt tggcgctggg
     1081 tgaaacaggc cacagattgg gatattgtca cgggcgatgc caacccgctg gccaactcca
     1141 gggcggtggc cggccgccag cgatggcgcg gcggggcttt gcccgactat ttcgacgttg
     1201 gcgccgacgc agccggcaaa gccttgcgct ttacggtaga gctcaagcac gccggccccg
     1261 aaggcttgac gatcaccacg cgcagcgcgg ctgaaacttt ttttcaacga cccgagccgc
     1321 gcactgaccg gcgtcttgaa agcagtaatt tattccattc atattggcag gcccgattgg
     1381 catccccgaa cgatttggcg gatggttcaa tgacagatgc caaagggttc ccatgaagcc
     1441 agctcgatcc atgcagccgc atttgcgcct ggggttggga cttttcgcgg ccaggcgcgg
     1501 ggtatcccgg cgatctccgt taaatgcttt ctccgtatgc cggtttggca tggaccggca
     1561 ttccaaagcg cggctgttgg cgtgccgatc gcgttcaata catacgattc atgcgcggca
     1621 ggccggacaa gctctggtcg agggaatagt gattttgttg gctctgctgt cgttgtgggt
     1681 tgccatttca tggttaggcc gctttcagga tatggccctg caagcgtctc acgcaagccg
     1741 gtttgcggcc ttttcgctaa ctcgcaaccc catcgcgcca cccgtacttc aaggccgtac
     1801 tgactatttt tcaggaccgg atcatcaatg gatggaccgg cggggccaaa gtgcgctaag
     1861 caaggatcgt tctgaagtga cgttcagtgt tggtcgtcaa ccggtcctgc aatccggcgc
     1921 tcaacctggc ggcgcggcgt cgcgcgcaca agaattgcgg caaggctggc gtcttgaaga
     1981 tacaggtatt ctaaaggcct atgcgatagt ctcatcttcg cactatcctg ctttggctcg
     2041 ccatactgcg ctactaacgg gcgctggcca tgcctcggac gacgccgata accagcatcg
     2101 gctggctgag tcgcccgccg catggagcga cagcgcaaat ccttcttatg agctcggccg
     2161 gcgaattgaa tcgcgcgtga gcgcgcttga tcgggcttgg ggcaggtcac agccgcattt
     2221 tgattggctc ggcccttggg caggttatgt gccggcccat catcttggcg aatttcaagg
     2281 ggatgacaag tgatcaggat gctcgtttgt tccctgctga tggtcacggc gtatttgggt
     2341 gttccgcaac aagccatcgg ttcaatctcg gcgcagttcg caactcttga atccgagcct
     2401 ctggtctggt ccgagcgcca ggaaatgcgg ttattcggtg tgcccgtctt tcaacgctcc
     2461 ttttcatcaa tggaatccaa acaaaacgtt gcggaaatcc tggcggcgca taccgatatg
     2521 tttcagcggg ctgtggcgtt caggaacaag acagtgctct ctggtgcaaa gaccggttgg
     2581 cattgggtcg cggagatcga ttcgacggat ttcggcgctc gcggatcggt atcggcgcta
     2641 cgcatcggcg gccccgaact gaaccacgct gtggccattc agtccaaacc cggttttagc
     2701 tggttgccgc aacaggcgaa gcggcgcttc agtcagcgta cggtcgcgcg ggatcgacaa
     2761 gttctggacg aggtctacag tactgcattg cgtcccgaag cgttgcttgg atatctgcaa
     2821 aagcagttgc gcctcagggg ctgggtcgat gagccaggtc tggcgtggtt gcccaacatg
     2881 agcgtgtggc ggcgcaaggc gtccgagctc acattgattt cgagcgccag ccgcgatggc
     2941 agcgccgtgt tcattcatta tctcgagtag accggctatg aatattttca cgcgtttgcc
     3001 tgcattcaat cgatcggctg ccatgtttgt gcttgctctg atcgctggct tgatcgc
//
LOCUS       DATCXU010000357         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_921003,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000357 DATCXU010000000
VERSION     DATCXU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921003"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /gene="mrdA"
                     /locus_tag="VIR04_08050"
     CDS             <1..2028
                     /gene="mrdA"
                     /locus_tag="VIR04_08050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY9460737.1"
                     /translation="KRRVVLRVWIVGVFALLCFGVLVARLWYLQVVRYEGLAERANQN
                     RIAVVPIPPRRGIVVDRNGVVLARNYRDYTLEVVPARVENMPKMLDALGQLVYLSASD
                     RKQFKLNLNRTGRYSHVLLRNNLNDTEAFWFAAHSYKFPGVELRARWVREYPQGESAA
                     HVLGYVGRLSEADMARLDEEGQAGNYRGTDIIGKQGIEKTWENVLHGKTGIEEVEVTA
                     SGRPVRTLHRIDPVPGSNLVLSLDIGLQQEVEKQFEGKRGALVAIDPKTGDVLAFVSA
                     PSFDPNLFVDGIDVEHWRELNDSPDHPLINRPLYGTYPIGSTYKPFVGLAALELGKRS
                     ATEKIRDPGYFELGGQRFRNAGGVAYGPTDMHRAIVKSSDTYFFSLGPEIGVDALHDF
                     TKQFGFGQITGIDLDGERRGVLPSKEWKRKAYKTRAQQHWYVGESVSIAVGQGYNAFT
                     LLQLAQATSVLANNGVYMKPHLVGLVENAQTGETVPTVTKPSYTIHLKPENLKVIQDA
                     MADVTRIGTARSAFVGAPYQAAGKTGTAQVYSLKGAKYHAGAVNERLRDHALFIAYAP
                     FDNPRIAVALIVENGGWGATVAAPIARKVFDYWLSPDRNKNQNEVSLPPSLAARVNSL
                     KPLDDGAVGESPDALVRTEDLPDNPDAGDPDPLSDMTHTGTLPDEQGRPTQ"
     gene            2025..3161
                     /gene="rodA"
                     /locus_tag="VIR04_08055"
     CDS             2025..3161
                     /gene="rodA"
                     /locus_tag="VIR04_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY9460738.1"
                     /translation="MKRIIQYALRAIAAFDWPLLALLVLMCLLGLTVMHSAVGGTDWR
                     FADQSRNFLIAFVFMWIAALLPPPTLMRLALPSYIAGIVLLLGVEFFGETSKGATRWL
                     SIGFTRIQPSEMMKIAVPMMLAWYFHRKQGKIGVVDFFVAAVLLLAPFGLIVKQPDLG
                     TALLVFTAGFCVIYLGGLSFKLLAPVAVVAVVGLGTLGYYQTQICQPDVDWVVLHDYQ
                     KSRVCTLLDPSSDPLGKGFHTIQSMIAVGSGGLYGKGYMQGTQSHLDFVPERTTDFIF
                     AVYAEEFGLYGGVMLLVLYSLVVLRGLTIAVRAHTQFGRLMAGAMSMMFFVYVFVNIG
                     MVTGILPVVGVPLPFMSYGGTAVSTLGVACGILMSISRYRPARR"
BASE COUNT          635 a          822 c         1019 g          826 t
ORIGIN      
        1 aaacggcggg ttgttttgcg tgtctggatt gttggcgtct ttgcactgct gtgtttcggg
       61 gtgctggttg cacgcctttg gtatctgcag gtcgtccgct acgaaggtct tgccgagcgc
      121 gccaatcaga accgtattgc cgtagtgccg attcctcctc gtcgcggtat tgtcgttgac
      181 cgcaatggcg tggtgttggc cagaaactat cgtgattaca ctctcgaggt cgtgccggcc
      241 cgggttgaaa acatgcccaa gatgctggat gcgctggggc aactggtcta tttaagcgcc
      301 tcggatcgaa aacagttcaa gctgaatttg aaccgcacgg gccgctattc gcatgtattg
      361 ttgcgcaata atttgaatga caccgaagcg ttctggtttg cggcgcattc gtataaattt
      421 ccgggtgtgg aattgcgtgc ccgctgggtg cgcgagtatc cgcagggcga atcggccgcc
      481 catgtgcttg gatatgtggg ccgcttgtcg gaagcggata tggcccggct cgacgaggag
      541 ggccaggccg gcaattatcg cggcaccgac attatcggca aacaaggcat tgaaaagacc
      601 tgggaaaatg ttttgcatgg caaaaccggt atcgaagagg tcgaggtcac cgcttcaggc
      661 aggccggttc ggactttgca tcgcatcgat cccgtgcccg gttccaatct ggttttatcg
      721 cttgatatcg gcttgcaaca agaagttgag aagcagtttg aaggcaaacg gggcgcattg
      781 gtggcaatcg acccgaaaac tggcgacgtg ctggcgtttg tgtcagcgcc gtcatttgat
      841 cctaatttat ttgtcgatgg catcgacgtg gagcactggc gcgagctgaa cgattcaccc
      901 gatcatccgc tgattaatcg ccctttgtat ggcacttatc ccatcggttc gacatacaag
      961 ccatttgtgg ggctggcggc gctcgaactt ggcaagcgtt cggcgaccga gaaaattcgc
     1021 gatcccggct atttcgaact gggagggcag cgttttcgca atgccggcgg cgtcgcctat
     1081 gggccaaccg atatgcaccg cgcgatcgtc aaatcttctg atacctattt cttttcgctc
     1141 ggtcccgaaa taggggtcga tgcgctgcat gatttcacca agcagtttgg ctttggacaa
     1201 attaccggaa tcgatctgga tggtgaacgg cgcggagtct tgccttcgaa agaatggaaa
     1261 cgcaaagcat ataaaacccg ggcccagcag cactggtacg ttggcgaatc ggtgtcgatt
     1321 gcggtgggcc agggttacaa cgcttttact ttgctgcaac tggcccaggc aacgtccgtc
     1381 ctggccaata acggcgttta tatgaagccg cacctggttg gtttggtcga aaatgctcaa
     1441 accggggaaa ccgttccgac cgttaccaag ccgtcataca cgattcacct caagcctgaa
     1501 aatttgaaag tgattcagga tgcgatggcc gacgttaccc ggatcggcac cgctcgcagc
     1561 gcgtttgtcg gggcgccata tcaggctgcg ggaaaaacgg gtacggcgca ggtctatagc
     1621 ctgaagggcg cgaaatatca cgctggcgcc gttaacgagc gtctgcgcga ccatgccttg
     1681 ttcattgctt atgcgccgtt tgataacccg cgcatagctg tcgctctgat tgtcgagaat
     1741 ggcgggtggg gcgcgacagt ggcggcgccc atcgcgcgca aggtgtttga ttattggctt
     1801 tcgcctgatc ggaacaagaa ccaaaatgaa gtaagcctgc ctccgtcgct agcggcgcgg
     1861 gtaaattcgc tcaagccgct tgacgatggc gcggtgggcg aatctcccga tgccctggtg
     1921 cgcactgagg atctgccgga taatcccgac gccggcgacc cggatccttt gtcggacatg
     1981 acgcacaccg ggacgttgcc ggatgaacaa gggcggccaa cgcaatgaag cgaattatcc
     2041 aatatgcgct cagggcgatt gccgcgttcg actggccgct gcttgctttg ctggtgctca
     2101 tgtgtttgct gggtttgaca gtaatgcact cggccgtggg gggaaccgac tggcgctttg
     2161 cggatcagtc ccgcaatttt ttgattgcgt ttgtttttat gtggatcgcc gctctgttgc
     2221 cgccgcccac gttaatgcgc ctggcgttgc cttcttatat tgcaggcatt gttcttttac
     2281 tgggcgtgga gttttttggt gaaacgagca aaggagccac tcgctggcta agcattggtt
     2341 ttacccgaat tcagccatcc gagatgatga agatcgcggt gcccatgatg ttggcctggt
     2401 atttccatcg caagcaggga aaaatcgggg ttgttgattt ctttgtcgct gctgttttgc
     2461 tgctggcgcc ctttggactt atcgtaaagc agcccgatct gggcactgca ctgcttgtgt
     2521 ttacggctgg gttttgcgtt atttacctgg gcggtttgtc gttcaagctg ttggcgccgg
     2581 tggcggtggt ggcggtggtg ggcctgggca cgctggggta ttaccaaacg cagatatgcc
     2641 agccggatgt ggattgggtg gttttgcacg attaccaaaa aagccgggta tgcaccttgc
     2701 tggatccgag ttccgacccc ttgggcaagg ggttccacac catacagtcg atgattgcgg
     2761 tgggatctgg cgggctctac ggcaagggat atatgcaggg cacgcaatcc catctggact
     2821 ttgttcccga gcggaccacc gatttcatat ttgccgtata cgctgaagaa tttggccttt
     2881 acggaggggt gatgttgctg gtgttgtact ccctggtcgt tttgcgtgga ctgacgattg
     2941 ccgtgcgggc gcatacccaa ttcggccgct tgatggcggg cgccatgtcg atgatgtttt
     3001 ttgtttatgt gtttgtgaat attggcatgg ttacgggaat tctgcccgtt gtgggcgtcc
     3061 cattgccttt tatgagctac gggggcacgg cggtgtcaac actgggtgtg gcgtgcggaa
     3121 tactgatgag catcagtcgc tatcgccccg cgcgtcgtta gcgtctgttg cgcggcgggt
     3181 cttcagtcaa acctgacaat agtttgctga tgggtgcggg tagcgcagtg gtagacaaat
     3241 catgaatgga aacccatgcc tgttgcgtcg ccggccgggc ggcaagggga gcgcggcata
     3301 cg
//
LOCUS       DATCXU010000358         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_188707,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000358 DATCXU010000000
VERSION     DATCXU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188707"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..690)
                     /locus_tag="VIR04_08060"
     CDS             complement(241..690)
                     /locus_tag="VIR04_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9460739.1"
                     /translation="MPPARINTEIVARSMGEPEGGPSAVQRAAETYCEMLGFLPPRVE
                     ARLHVTGALDPALLALQETIRSHAMNPACFDPKTVQLMVFGMLMVDLSDAATIHGIAA
                     RRAGASWEEMQAVVSLAYLFRGVSAANRGADVLARIAEREAQQPDHA"
     gene            828..1511
                     /locus_tag="VIR04_08065"
     CDS             828..1511
                     /locus_tag="VIR04_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY9460740.1"
                     /translation="MKNSAELPTVRAGASLTAALAIQIRAEILQGSYPPGSKLRLDDL
                     RLQYAVSLSPLREALTRLTAEGLVQTTDHRGYRVAPVSVENLCEVTTLRTQMEVMAMT
                     ESIRQGDDDWEDALTAAYYRLARLEKKGERSDEWEQTHRVFHLALFSSCNMPLLLRFC
                     GMLHDLSDRYRRLFLAHHRPDKKVPAEHKAIFEAAIARNETLAAQILTQHIQRTGLNV
                     KAILAEKKP"
     gene            complement(1600..1675)
                     /locus_tag="VIR04_08070"
     tRNA            complement(1600..1675)
                     /locus_tag="VIR04_08070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1640..1642),aa:Val,seq:tac)
     gene            complement(1764..2036)
                     /locus_tag="VIR04_08075"
     CDS             complement(1764..2036)
                     /locus_tag="VIR04_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY9460741.1"
                     /translation="MNKTELIEHISTKADLSKADAGRALEAFIGAVKTTLKKKGTVTL
                     VGFGTFAVSERAARTGRNPRTGEAIKIKKAKVPKFRPGKALKDAVN"
     gene            complement(2394..3629)
                     /locus_tag="VIR04_08080"
     CDS             complement(2394..3629)
                     /locus_tag="VIR04_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEY9460742.1"
                     /translation="MAKNGFTTTILHSDRRGLAEHGAVHKPMHSSCEYGYSDSRELAA
                     VFQGKPGFTYARQGTPTTSALEVIVTQMEGGANTVSFATGMAALSATIFPLLRRGDHL
                     ITSQYIFGNSNSLFNTLASLGVEVTLVDATDVANVIAAWRPETKMVFTETIANPGTQV
                     ADLAAIGAWCKEKHLVYVVDNTLTSPWLCRGIDFGASLVVNSLSKLIGGHGDALGGAV
                     TDTGLYDWSDYQNIAEPYRKGNPTTWGLYQIKKKGLRDMGGTLSSESAHRISVGAETL
                     ALRMDKSCSNAMALARFLDQHPKVAKVTYPGLEKHAQHKRATELFNHRFGALMGVDLI
                     DGIDVFDFLNRLKVLILATHLGDTRTLVLPMAQTIYYEMGPQRRALMGISDTFMRISV
                     GIEDTQDLIDDFTQALDAS"
     gene            complement(3687..4436)
                     /gene="rlmB"
                     /locus_tag="VIR04_08085"
     CDS             complement(3687..4436)
                     /gene="rlmB"
                     /locus_tag="VIR04_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY9460743.1"
                     /translation="MSSQQVLAGFHAVVARMRHAPASIKELYVEASRHDKRMQSFIEQ
                     AQAANLRIHPVPADRLDGLSHGVKHQGVVALADEAELAVDVDEVLDMLEDKNQAALFL
                     ILDGVTDPHNLGACLRTADAAGVHAVIAPRDRAVGLNSTVRRVACGAAESVPYIMVTN
                     LARTMRQLKSRDVWLMGTDDQASRNVYQTDARRAMGWVLGAEGEGMRRLTRETCDELV
                     KIPMLGSVESLNVSVASAVCLYETVRQRSPA"
     gene            complement(4516..>6160)
                     /gene="rnr"
                     /locus_tag="VIR04_08090"
     CDS             complement(4516..>6160)
                     /gene="rnr"
                     /locus_tag="VIR04_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY9460744.1"
                     /translation="ATVKRAELSERIDLREVPFVTIDGEDARDFDDAVFGMPLELGAE
                     KRKKPGWRLLVAIADVGHYVRPGDALDSDALERGTSVYFPRRVIPMLPEALSNGICSL
                     NPDVDRLVMVCDMVIPATGPKAGTVTAYQFYNAVIHSHARTTYTDVWTALQQPAGPAA
                     QAMKEVLPHIQNLYELFHLFAQARVKRGAIDFDTVETKIICNPLGRIEKIVPYVRNDA
                     HRLIEECMLAANTCAADFMARNKRMGLYRVHEGPTPEKLKALRDYLRNIGLTLDGGDD
                     PSTHDYARLIQAASSRPDFDILQTMCLRSLQQALYSPEQMGHFGLAYAHYSHFTSPIR
                     RYPDLLTHRVIKSILEGKRYVPRLDEPGTAAADLPKDEREHAVWEKLGVLLSARERRA
                     DDASHDVEAWLKCWFVKEHVGEVFSGHVTGVASFGVFITLDTLFVEGMVHVSELGSDY
                     FQFNEAMHELRGERTGMRYRLTDAVQVQVARVDLEARRIEFRLVKGTSFKALKAAIEP
                     AEAPVRRVKRAASAKPEALKGTTSRERRAAAKNSGRKRH"
BASE COUNT         1390 a         1786 c         1672 g         1312 t
ORIGIN      
        1 tgccatgcgc gccgccatgc acgatgaccg cgcgatcaat gccaagggct tttagcaagg
       61 cttcataatc ctgcacagtg caatcaactg gtgtatagct gcgctgtgca gaaaatgggt
      121 aacgcgcttc agggccgaaa atgtgggcat gacaatcgca tgcacccgct ggcatacgaa
      181 aaaccgggcg tttggtatgg cgatcagggc cggggcagtt ttgcaagttg gcgcgatgca
      241 ttatgcgtga tccggctgct gcgcttcgcg ttccgcgatc cgcgccagca cgtcggcgcc
      301 ccgattggcg gcggaaactc cgcggaacaa ataagccagg ctcacgacgg cctgcatttc
      361 ttcccagctt gcgccggcgc ggcgcgctgc aatgccatga atagtggccg catcgctcaa
      421 gtcaaccata agcatgccga agaccatcag ttgcaccgtc ttcgggtcaa aacacgcggg
      481 attcatggca tgagaacgga ttgtttcttg caaagcgagc aaggcgggat ccagtgcgcc
      541 cgtcacgtgc aatctcgcct ctactctggg aggtaaaaac cccagcatct cgcaataggt
      601 ctcggctgcc cgctgcactg ccgatgggcc gccttcgggc tcgcccatcg aacgagccac
      661 aatttcagta ttgattctag ccggtggcat acacacatcc ttgaagtcat gcggggggcc
      721 ctattgttta accaaaatat aagcaatgtc aacaaaaatc gattattctt aaataatcga
      781 ttatggccaa cacggctaaa ataatgcccg gaaataatgg tgtgtcgatg aaaaactctg
      841 cagaacttcc gaccgtgcgg gccggagcat cccttaccgc agcgctggct attcagattc
      901 gagccgagat attgcaagga tcgtatcctc ctggatcgaa gctgcgactg gatgacttgc
      961 gcctgcaata tgctgtaagt cttagcccat tgcgcgaagc gctgactcgc ctgaccgccg
     1021 agggactggt gcagaccaca gatcaccgcg gttacagggt tgcccccgtg tcggtcgaaa
     1081 acctgtgtga agtgactacc ctgcgcacac aaatggaagt gatggccatg actgagtcga
     1141 tcaggcaagg tgacgacgat tgggaagacg ccctgacagc ggcgtattat cgactggctc
     1201 gcctggaaaa aaagggggaa cgatccgacg aatgggaaca gacacatcgc gttttccact
     1261 tggccctgtt ttcgtcctgc aatatgcctt tgctgctgcg tttttgcggc atgctgcacg
     1321 acctgagcga ccgctaccgc cgcctgtttc tggctcacca ccgccccgac aagaaagtgc
     1381 cggccgagca caaggccatt tttgaagccg ccattgcacg caatgaaacg cttgcggcgc
     1441 aaatccttac acaacatatc cagcgtacgg gcctgaacgt gaaagcgatt ctcgcggaaa
     1501 aaaaaccctg attcctatcg gccgcgcctt gaaaacgctg gccgcaataa aaaaccgctt
     1561 agcttttggc taagcggttg ttttcatttg ggctttaagt ggtgggtgct acagggttcg
     1621 aacctgtgac ctacgccttg taagggcgcc gctctaccaa ctgagctaag caccccatgg
     1681 gcttttgggg ccacttcact tcttgcaaaa cgcgccgtag attgtagtcc acagcgcgag
     1741 cggccgcaaa ttgcaaaccg gatttagttg acggcgtctt taagcgcttt gcccggacgg
     1801 aactttggca cctttgcttt cttgattttg atggcttcgc cagtgcgcgg gttgcgaccc
     1861 gtgcgcgcag cgcgttccga cacggcaaat gtaccgaacc ccaccaacgt tacggtccct
     1921 ttctttttaa gggtggtttt cactgcgcca ataaacgctt cgagcgcgcg tccggcatcg
     1981 gccttggaca agtcagcttt agtggaaata tgttcgatga gttctgtttt attcattaag
     2041 gattacccct aaaaattatt gttgccaaac tcggcgcacg ggcttcgctt ggcgcctgac
     2101 ggaaaagtac ttgacactaa ttgtgctacc ggtgtaaccc acaacagatc aagccagtac
     2161 gaccgggtgg cactacagac gcaaaaatgc ctctccggtc ggctaaggcg tattaggcct
     2221 ttgaaagcct tgactgtcaa gcgaacaaac cctcaaaagc cgcgtaaatg ctcacttttt
     2281 tgcgtttgtg catcagtcat taacaaatct gttaaatata ttaagagatt gatccacagc
     2341 gcaatagaat gtggtaactg cgcactgtga acacacaaac ctgcccgaga tcattagctt
     2401 gcgtccagcg cctgggtgaa atcatcgatt aaatcctgcg tatcttcgat tccaaccgag
     2461 atccgcatga aggtgtctga aatccccatc aaggcgcgcc gctgcggacc catttcgtag
     2521 taaatcgtct gcgccatagg cagcaccagc gtgcgggtat cgcccaaatg ggtcgccaga
     2581 atcaggacct tgagccgatt caaaaaatcg aacacgtcga tcccgtctat aaggtccaca
     2641 cccattaatg cgccaaatcg atgattgaac aattcggtgg ctcgcttgtg ctgggcgtgc
     2701 ttttccaggc ccgggtacgt caccttggca accttggggt gctggtctaa aaatcgggcc
     2761 agcgccatgg cgttggaaca cgatttgtcc atgcgcaacg ccagcgtttc agcgccgaca
     2821 gaaattcgat gcgcggattc agacgagagt gtgccgccca tatcgcgcag gccttttttc
     2881 ttgatctgat acaggcccca tgtcgtcggg ttccccttgc ggtaaggctc ggcaatattc
     2941 tggtaatccg accaatcgta taagcccgtg tcagtgaccg cgccgcccag ggcgtctccg
     3001 tgtccgccga taagcttgga caaagaatta accaccagcg acgcgccaaa gtctatgccc
     3061 cggcacaacc atggagaagt caaggtgtta tcgacgacat aaaccaggtg tttttctttg
     3121 caccaggcgc caatggcagc caggtcggca acttgggttc cgggattggc gatggtttca
     3181 gtaaacacca ttttagtttc ggggcgccag gcggctatca cattggcaac atcggtggcg
     3241 tccaccaacg ttacttcgac tcccagcgat gccagggtat tgaacaagct gttggagttg
     3301 ccgaatatgt actggctggt gatcagatga tcgccgcggc gaagcaaagg gaaaatcgtt
     3361 gcggacaggg ccgccatgcc tgtggcaaaa ctgacggtat tggcccctcc ttccatttgc
     3421 gtcacgatca cttccagcgc ggaagtcgtc ggagttcctt gcctggcgta agtgaaaccc
     3481 ggtttgccct ggaatacggc agccagttca cgcgaatcgg aataaccgta ttcgcacgaa
     3541 gaatgcatgg gtttatggac tgcgccgtgt tcggccaagc cgcgccggtc tgaatgaaga
     3601 atagtggtag taaagccgtt tttagccatg gcagaatcaa tgcttaaaaa gttggaagaa
     3661 ataaatagca ggaaaaacga aacggtctat gctggcgatc gctgacgcac cgtttcgtac
     3721 aaacacacgg cgctggcgac actgacattc aggctttcga ccgaacccag catgggaatc
     3781 ttcaccagct cgtcgcaggt ttcgcgggta aggcggcgca tgccctcgcc ttcggcgccc
     3841 agcacccacc ccatcgcgcg gcgcgcatcc gtctggtata cattgcgcga agcctgatca
     3901 tcagtgccca tcaaccacac gtcgcggctt tttaactggc gcatcgtgcg agccagattc
     3961 gtaaccataa tataaggaac cgattcggcc gcgccgcagg ccacgcggcg cactgtgctg
     4021 ttcaggccca ccgcgcggtc gcgcggcgca atcacggcat gcacaccggc cgcatcggcg
     4081 gtgcgcagac acgcacccag attatgcggg tcggtaacac catccagaat cagaaacaag
     4141 gcggcctggt ttttgtcttc gagcatgtcc agcacctcgt cgacatcaac ggccaactcg
     4201 gcttcgtcgg ccagggcgac aaccccctga tgtttgacgc cgtgcgacaa accatcaagg
     4261 cgatcggccg gcaccggatg aatccgcaga ttggccgcct gggcttgttc aataaacgat
     4321 tgcatgcgct tgtcgtggcg cgacgcttcg acatagagct ctttgatgga agccggtgcg
     4381 tggcgcattc gtgccacgac tgcatggaaa cccgccagga cctgttgcga cgacatactc
     4441 tttctttcaa ccttgttcag ttttcaaaat acaaaccaac ttcgacgcgc ccgactggct
     4501 gcgcaaccca agcggtcaat ggcgcttgcg gccggaattt ttagcggccg cccggcgttc
     4561 gcgcgaagtc gtgcctttca gcgcttcggg tttggccgaa gcggcccgct taacgcgccg
     4621 cacaggcgcc tcggccggtt caatcgccgc cttgagcgcc ttgaaactcg tgcctttgac
     4681 cagtctgaat tcaatgcgcc gggcttcgag atcgacgcgg gccacctgaa cttgcaccgc
     4741 atccgtcagg cggtaacgca tgcccgtgcg ctcgccgcgc agctcgtgca tggcttcgtt
     4801 gaactgaaaa tagtcggaac ccaattcaga cacgtgaacc atgccttcca caaatagcgt
     4861 gtcaagggtg ataaacaccc cgaaactggc cacgccagtg acgtgaccgc taaagacctc
     4921 gccgacgtgt tctttaacaa accagcactt tagccaagcc tcgacatcgt gcgaggcgtc
     4981 gtcggcacgg cgttcccgag ccgacaaaag tacgcccaat ttttcccaca ccgcatgctc
     5041 ccgctcatcc ttgggcaggt cggccgcggc ggtcccaggc tcgtcgagcc gcggcacata
     5101 acgcttgcct tcaagaatcg acttgatcac cctgtgcgta agcaaatccg ggtaacgccg
     5161 aatgggggat gtaaagtgcg agtagtgcgc ataagccaag ccgaagtggc ccatttgctc
     5221 ggggctgtat agcgcctgct gcaacgagcg cagacacatg gtttgcaaaa tatcaaaatc
     5281 aggccggctg gatgccgcct gtataagacg cgcataatca tgggtcgacg ggtcatcgcc
     5341 gccatcaagc gtcaagccga tattgcgcag ataatcgcgc agggccttga gtttttccgg
     5401 ggtcggcccc tcgtgcacac ggtacaaacc catgcgtttg ttgcgcgcca tgaagtcggc
     5461 cgcgcaggta ttggcggcca gcatgcattc ttcaatcaaa cggtgcgcgt cgttgcgaac
     5521 atagggcacg attttttcaa ttcgccccag cgggttgcaa ataatctttg tttccaccgt
     5581 gtcgaaatca attgctcctc ttttaacgcg ggcctgcgca aacagatgga acagctcgta
     5641 caagttctga atatgcggca gcacctcttt catggcctgc gccgccggac cggccggctg
     5701 ctgcagcgcc gtccagacgt cggtgtaagt cgtgcgcgcg tgcgaatgga tgacggcgtt
     5761 ataaaactga tacgccgtta ccgtgcccgc cttgggaccc gttgccggta tcaccatatc
     5821 gcacaccatg accagacgat ccacgtcggg gttaagggaa cagatgccat tggacaaggc
     5881 ttcgggcagc atcggaatga cccggcgcgg gaaatacacg ctggtgccgc gttccagcgc
     5941 gtcgctatcc agcgcatcgc ccgggcgcac gtaatgacca acgtcggcaa tcgcaaccag
     6001 cagccgccag cccggtttct tgcgcttttc agcgcccaat tcaagcggca tgccaaacac
     6061 ggcgtcgtca aaatcgcgcg catcttcgcc gtcgatggtg acgaagggaa cctcgcgtaa
     6121 atcgatgcgt tcgcttagtt ccgcacgctt taccgtggcg
//
LOCUS       DATCXU010000359         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_214956,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000359 DATCXU010000000
VERSION     DATCXU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214956"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIR04_08095"
     CDS             <1..189
                     /locus_tag="VIR04_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460745.1"
                     /translation="IGPTVGATILTILPEILRPFRDYRLAINGLILILVVLYLPSGIW
                     DPRRIHAFWQRRKTRRGR"
     gene            189..950
                     /locus_tag="VIR04_08100"
     CDS             189..950
                     /locus_tag="VIR04_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460746.1"
                     /translation="MLQFNQIGKDFGGLKVLENVSFDVNEGSIFGLIGPNGAGKTTVF
                     NLATGLLHATTGHIDFDGKSLDKLPPHQITALGIARTFQNIRLFKDASLLENVVIGMH
                     GHLNYSFADLLFNTPSFRRIEAAARERALELLSWLSLDDKADMLAGVLSYGEQRKLEL
                     ARALATQPRLLLLDEPVAGMNSVEKAELMVQILNIKKRGFSIFLIEHDMRFVMGLCDR
                     VAVLNFGRIIAEGPPEEIRVNPEVIEAYLGKEEDE"
     gene            947..1660
                     /locus_tag="VIR04_08105"
     CDS             947..1660
                     /locus_tag="VIR04_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460747.1"
                     /translation="MSEILRVVDLAVSYGHIEALKGIDLALNEGEITALVGANGAGKS
                     TTLMAISGLLKSVRGQVWLEDTDLSKLSPHRIVRNGVVQVAEGRAILTTLTIQENLEL
                     GAYTRNDKTEVARDMDWVYTLFPVLRQRAGGLAGNLSGGEQQMLAIARALMAKPRVLL
                     LDEPSMGLAPLLVREIFRVLKEINGTGLTIFLVEQNVRQALRIAQRGYVLETGKIVLS
                     DSGQNLLHNPRVIEAYMGG"
     gene            1765..>2070
                     /locus_tag="VIR04_08110"
     CDS             1765..>2070
                     /locus_tag="VIR04_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY9460748.1"
                     /translation="MKKVLGIYSGPAAHWVGDGFPVRSLFSYGDLGKHLSPFLLLDYA
                     GPADFTPASRPRGVGQHPHRGFETVTIVYKGEVEHRDSTGGGGIIGPGDVQWMTAASG
                     "
BASE COUNT          453 a          581 c          593 g          443 t
ORIGIN      
        1 atcgggccga ccgtgggcgc aaccatactg acaatactgc ccgagatact gcgcccattc
       61 cgggactatc gccttgccat taacggcctg atccttattc tggtggtctt gtacctgcca
      121 agcgggatct gggatcctcg ccgcatacat gcgttctggc aacgacgcaa aacacgcaga
      181 ggccggtaat gctgcaattt aatcaaattg gcaaggattt cggtgggctg aaggtccttg
      241 aaaatgtttc cttcgatgtg aacgagggca gcatttttgg tctgatcggc cccaacggcg
      301 cggggaaaac caccgtattc aatctggcca ccggtctgct tcacgcgaca accggccaca
      361 tcgatttcga cggcaagagc ctggacaaac tgccgccgca tcaaatcacc gcgttgggca
      421 tagcccgcac gtttcaaaac attcgcctgt tcaaagacgc aagcttgctg gaaaatgtgg
      481 ttattggcat gcatggtcac ctgaactaca gttttgcgga tctgttgttc aacacaccct
      541 ctttccgacg catcgaggca gcggcgcgcg agcgcgctct ggaactcttg tcgtggcttt
      601 cacttgacga taaagccgac atgctggcgg gcgtgctgtc gtatggcgaa cagcgcaaac
      661 tcgaacttgc gcgggcgctg gccacccaac ccaggctgct gctgctcgac gaacccgtcg
      721 cgggaatgaa ttcagtcgaa aaagcggagc tgatggttca gattctcaat atcaaaaaac
      781 ggggattcag cattttcctc attgaacacg acatgcgctt cgtgatgggt ttatgcgacc
      841 gtgtggcggt gcttaacttt ggtcgcatca tcgccgaagg gccgcccgag gaaatccgcg
      901 tcaaccccga agtgatcgaa gcctatctgg gcaaggaaga ggacgaatga gcgagatttt
      961 gcgcgttgtc gacctcgcgg tttcgtacgg ccacatcgag gcattgaaag gaatagacct
     1021 ggccctgaat gagggcgaaa tcactgccct ggtgggggcc aacggagccg gcaaaagcac
     1081 cacattgatg gcgatctcgg gtctgctgaa atccgtccgc ggccaagtct ggctggaaga
     1141 caccgatctg agcaagcttt cgccccatcg gatcgtacgt aatggcgtcg tgcaagtcgc
     1201 tgaaggccgt gcgattttaa cgaccctgac cattcaggaa aatctggagc ttggcgccta
     1261 cacccgtaac gacaaaaccg aagtcgcccg cgacatggac tgggtatata cgctgtttcc
     1321 agtactcagg cagcgcgccg gcggactggc cggcaacctg tctggaggcg aacagcaaat
     1381 gctggccatt gcgcgcgccc tgatggccaa gccgcgtgtt ctgttgctcg atgagccctc
     1441 gatgggcctg gcgccgctcc tggtgcgtga aatcttccgt gtgttgaagg aaattaacgg
     1501 tacgggcctt acgatattct tggtcgaaca aaacgtacgg caggcgctgc gtattgccca
     1561 gcgtggatac gttctggaaa ccggcaagat tgtgctgtcc gacagcggcc agaacctgct
     1621 gcataacccc cgggtcatcg aagcctacat gggcgggtaa ttgggccatt gttcttcacg
     1681 taggactcag atagcggttt ccatgatcta ccgcaggacg tatagaacac ctatgatgta
     1741 gtgatagtcc ataggagaac cctcatgaaa aaagtccttg gtatttattc aggccccgcc
     1801 gcgcactggg tgggcgacgg tttccccgtc cgctcgctat tttcttatgg cgacctcggc
     1861 aagcatctga gtcctttctt gctgctcgac tatgccggcc cagccgattt cactccggcc
     1921 tcacggccac gcggcgtggg ccagcatcca catcgcggat tcgagacagt cacgattgtc
     1981 tacaaaggcg aagtggagca tcgtgactca accggcggtg gcggcataat tggccccggc
     2041 gacgtgcagt ggatgacggc ggcctctggc
//
LOCUS       DATCXU010000360         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_427373,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000360 DATCXU010000000
VERSION     DATCXU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427373"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VIR04_08115"
     CDS             <1..444
                     /locus_tag="VIR04_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY9460749.1"
                     /translation="EPHRTEFIQTIGGIDFVNDSKGTNVGATVAALEGMDQKAVLIAG
                     GLGKGQDFSPLAKAVKEHARAVVLIGRDGPLIEQALADAAVPMQRAQSLEQAIAQCFA
                     LARQGDVVLLSPACASMDMFKNYRHRGAAFVEAVYALARDKGEVA"
     gene            441..1634
                     /gene="ftsW"
                     /locus_tag="VIR04_08120"
     CDS             441..1634
                     /gene="ftsW"
                     /locus_tag="VIR04_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY9460750.1"
                     /translation="MSLFAELTSGVNAVRPGRTTMPSFDVSLVIVVSALVLFGLVMVY
                     SASIALADGPRYQSYGRFYFVGRHALFIFIGFICALIAVNIPLAIWQKLALPMFLAAL
                     FFLLVVLVPGIGREVNGARRWLSLGPINFQPSELMKVMVLLYAADYTVRKQQHMHNFF
                     RGFLPMACALVAVGFVLLLEPDLGAFMVIVAIAVGILFIGGINGKLFSSLLGILVFSF
                     LLLIWLSPWRRERLFVYLDPWNPHNTYGSAYQLSHSLIALGRGEWFGVGLGASIEKLH
                     YLPEAHTDFIVAVIGEELGFVGLMCLISLFVFVVWRGFEIGRQAIAMERVFNGLVAEG
                     VALWFGVQTFINIGVCVGLLPTKGLTLPLVSYGGSGIVMNLIAVALLVRVDIENRNLM
                     RGKRT"
     gene            1631..>2605
                     /gene="murG"
                     /locus_tag="VIR04_08125"
     CDS             1631..>2605
                     /gene="murG"
                     /locus_tag="VIR04_08125"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY9460751.1"
                     /translation="MSARILLIMAGGTGGHIMPGLAVAHEMRDRGWRVVWMGHPERME
                     GRLVPAQGFELAPMRFSGVRGKGVMALLKLPFTLGGACCQAWKCLKKIKPDVVLGMGG
                     YPAFPGGLMAALRRTPLVIHEQNAVAGSANRLLARVAKKVLVGFPGALRGAVMVGNPV
                     RRELAQVAPPAQRYAARQGSLHLLVIGGSLGAHILNTVVPQALAQLPSHDRPVVVHQA
                     GEQHLTSLRAAYAVAGVQAQCHAFIDDMASVLADADLLICRAGAMTVAEVAAVGVAAL
                     FIPLPHAIDDHQTVNAHYLSDCQGAWQQAQSEFSASWLAQWLLQRTRAE"
BASE COUNT          432 a          669 c          850 g          654 t
ORIGIN      
        1 gagccgcatc gcaccgagtt catacagacg attggcggta ttgacttcgt caacgacagc
       61 aagggcacga acgttggcgc taccgttgcc gcgctcgagg gcatggatca gaaagcggtg
      121 ctgattgcag gcggtttggg caagggccag gatttttcac ctcttgccaa agcggtcaag
      181 gaacatgcgc gcgctgtggt gctgatcggg cgcgatggcc ccctcataga acaggcgctg
      241 gctgacgcgg ccgtgcccat gcaacgagcg cagtccctgg agcaggcgat tgcccagtgc
      301 ttcgcgctgg cgcgtcaagg tgatgttgtg ttgctgtcgc cggcatgcgc cagcatggat
      361 atgttcaaaa actatcgtca ccgtggtgcc gcttttgttg aagccgtgta tgcgctggcc
      421 agggacaagg gggaggtcgc atgagcctgt tcgccgaact tacctcgggt gtcaacgccg
      481 tgcggccggg ccgcacgacg atgcccagct ttgatgtttc cctggtgatt gtcgtaagcg
      541 cgctggtgtt gtttggtttg gtcatggtgt attcggcgtc cattgcgttg gccgacgggc
      601 cacgatatca gagctatggc cgtttttatt tcgttggccg ccatgcgctg tttattttta
      661 ttggctttat ctgcgccctg attgcggtga atattcctct ggccatttgg caaaagcttg
      721 cgctccccat gtttttggcg gcactatttt tcttgctggt ggtgcttgtg ccggggatcg
      781 gccgtgaagt gaatggcgca cggcgctggc tatcgcttgg cccgattaat tttcagcctt
      841 ccgagctgat gaaagtgatg gtgctgctgt atgccgctga ttacacggtg cgcaagcagc
      901 aacacatgca taatttcttc cgcggatttt tgccgatggc atgcgcattg gtggccgtcg
      961 gttttgtact gctgctggaa ccggatttgg gcgctttcat ggtgattgtc gccattgccg
     1021 taggaatttt atttatcggc ggcatcaacg gtaaattatt ttccagtctg ctgggtattt
     1081 tggtgtttag cttcctgctt ttgatctggt tgtcgccttg gcgtcgcgag cgcctgtttg
     1141 tgtatctgga cccatggaat cctcacaaca cctatggcag cgcctaccag ttatcgcact
     1201 ccctgattgc tctggggcgc ggcgagtggt ttggcgtcgg cctgggggca agcattgaaa
     1261 aactgcacta tttgcccgag gctcataccg atttcattgt ggcggtcata ggcgaggagc
     1321 ttggttttgt cggactcatg tgcctgatca gcctgtttgt ttttgttgtc tggcgcggct
     1381 ttgaaatcgg ccgccaggcg atagccatgg aacgtgtatt taatggtctg gttgccgagg
     1441 gcgtggcctt gtggtttggc gttcaaactt ttatcaatat cggcgtgtgc gttggcttgt
     1501 tgccgaccaa aggtcttaca ttgcccttgg tcagctatgg cggttcggga attgtaatga
     1561 acctgatcgc cgtggccttg ctggtgcgcg tggatattga aaaccgcaat ttgatgcgag
     1621 gcaaacgaac atgagcgcgc gcatcttgct gattatggcg ggcggcaccg gcgggcacat
     1681 catgccagga ttggcggtgg ctcacgagat gcgggatcgc ggctggcgcg tggtatggat
     1741 ggggcatccc gaacgcatgg aagggcgact ggttccggcg caagggtttg aactggcgcc
     1801 catgcgcttt tcaggggtgc gcggcaaagg cgtgatggcc ttgctgaaac tgcctttcac
     1861 gttggggggc gcgtgctgcc aggcttggaa atgcctgaag aagatcaagc ccgatgtggt
     1921 gctcgggatg ggtggctacc ctgcctttcc gggtggactg atggccgcgc tacggcgtac
     1981 ccctcttgtc attcatgaac aaaacgccgt cgccggaagc gcgaatcggc tgttggctag
     2041 agtagcgaaa aaggtgttgg tcggctttcc gggcgcgttg cgcggcgctg tcatggtcgg
     2101 aaaccctgtc aggcgcgaat tggctcaggt tgcgccaccg gcgcagcgct acgccgctcg
     2161 gcagggatcg ctgcatctgc tggttattgg cgggagcctt ggcgcccata tcctgaacac
     2221 agtggtgcca caagccctgg cgcagttgcc ttcccatgat cgcccggtgg tggtgcatca
     2281 ggccggtgag cagcatttga caagcctgcg ggccgcctac gccgttgctg gcgtccaggc
     2341 gcaatgccac gcgttcatcg acgatatggc cagcgtgctg gctgatgccg atctgttgat
     2401 ttgccgggcg ggcgccatga cggtggcgga agtcgccgca gtgggcgtgg cggcattgtt
     2461 tattccgctg ccgcatgcga ttgatgatca tcagaccgtc aacgcgcatt acttgagcga
     2521 ttgtcagggc gcctggcagc aggcccagtc agaattttcg gcgtcatggc tggcgcagtg
     2581 gctactccag cgcacgcgcg cggag
//
LOCUS       DATCXU010000361         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1133940,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000361 DATCXU010000000
VERSION     DATCXU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133940"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /gene="murC"
                     /locus_tag="VIR04_08130"
     CDS             <1..180
                     /gene="murC"
                     /locus_tag="VIR04_08130"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814572.1"
                     /note="Catalyzes the formation of
                     UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate
                     and L-alanine in peptidoglycan synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY9460752.1"
                     /translation="DGRALARALRVAGKVEPLFVQDVADLPQAILDFVHGGDVVLVMG
                     AGSIGRVAARLGELA"
     gene            177..1142
                     /locus_tag="VIR04_08135"
     CDS             177..1142
                     /locus_tag="VIR04_08135"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY9460753.1"
                     /translation="MTNNFGRVGVLYGGRSAERQVSLMSGAGVHEALCNAGIDAHLFD
                     TGKRTLVDLINAGFDRVFIALHGRHGEDGTLQGALELIRLPYTGSGTLASSLAMDKVM
                     SKKVWLEQDLPTPGFTLLLSDADLDHAAGDLGLPLILKPPSEGSTLGVTKVTDRAQLR
                     DAYRLATQYDDTVLAEKFVEGREITVALLGKGKTARALPIIEIVAPQGNYDYEHKYVS
                     NDTQYICPAHLDTRLTQHILQLAERAYNALGCEGWGRADFMLDAQDRPWLLEINTSPG
                     MTSHSLVPMAAKAAGMSYMDLCVEILASASCKVQAPLEIDPEERI"
     gene            1145..1936
                     /locus_tag="VIR04_08140"
     CDS             1145..1936
                     /locus_tag="VIR04_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY9460754.1"
                     /translation="MFNDARLTNFIANMLALLAVLAMVAGVVIWVAQRPYFAITQIQI
                     EPMRSGSLNYVSATSVQATIAGRLIGNFFFVKLDDARKLIETVPWVRHARIQRVWPNT
                     LRVQIEEQQPLAYWNDNQMINSWGEAFSANQGELPDDLELPHLNGPANSERLVVQRYA
                     ELARWFAPLALSVANITLSPRYAWEVELSDGVRLNLGRDPAADVADPHGRSGALPFAA
                     RIERFVQEWPILAHRLGGRAISSADLRYSNGFAITLAPVTTTPSK"
     gene            1944..>2602
                     /gene="ftsA"
                     /locus_tag="VIR04_08145"
     CDS             1944..>2602
                     /gene="ftsA"
                     /locus_tag="VIR04_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY9460755.1"
                     /translation="MTRDIKDLIVALDIGTSKVVAVVAEILPEGRFEVLGLGQHESRG
                     MRKGVVVNIEATVNSIQRALEEAELMADCKIREVYTGIAGSHITSFNSSGMVAVKDKE
                     VTATDVARVIETAKAVNIPTDQQVLHVLTQEFIVDSQEDIREPIGMSGLRLEVRVHIV
                     TGSVSAAQNIVKCVRRCGLEVQDLILQPLASSLACLTSDEKELGVVLVDIGGGTTDIA
                     I"
BASE COUNT          541 a          673 c          806 g          582 t
ORIGIN      
        1 gacggacggg cgcttgcgcg cgcgttgcgt gttgccggaa aggtcgaacc tttgttcgtg
       61 caggatgtag ccgatcttcc gcaggccatt ctcgattttg tgcacggcgg cgatgtggtg
      121 ctggtaatgg gcgcgggatc aatcggccgt gtcgcggcac ggctcggaga attggcatga
      181 cgaataattt cggacgcgtg ggtgtgctgt acggcggccg gtcggcggaa cgccaggtgt
      241 cgctcatgtc tggcgccggc gtacacgagg ccttgtgcaa cgccgggatc gacgcccatt
      301 tgttcgatac gggcaagcgc actttggtcg atttgatcaa tgccggtttt gaccgcgtct
      361 ttattgcttt gcatggacgc catggcgaag acgggaccct tcaaggagcg ctggagctga
      421 ttcgattgcc gtacacgggc agcggcacgt tggcgtcaag cctggccatg gataaggtaa
      481 tgagcaagaa ggtgtggctt gaacaagatc tgcccacgcc gggtttcaca ctattgcttt
      541 ccgacgccga tctggatcat gccgccgggg acttgggctt gcctttgatt ttgaagccgc
      601 cttctgaagg gtcaacgttg ggtgtaacca aggttacgga cagggctcag ttaagggacg
      661 cgtatcggtt ggccacccaa tacgatgaca cggtcctggc ggaaaaattt gtcgaaggcc
      721 gcgagatcac cgtagcgttg ctgggcaaag ggaaaacggc cagggcgctg cccattatag
      781 aaatcgtggc gccccaggga aattacgatt acgagcacaa atatgtgtcc aacgacaccc
      841 agtatatttg cccggcacac ctcgacaccc gtttgactca gcacatcctg cagttggccg
      901 agcgggcata taacgctctg ggttgcgaag gatggggacg tgccgatttc atgcttgatg
      961 cgcaagatcg gccctggttg ctggaaatca acacctcgcc cggcatgaca agtcactcat
     1021 tggttcccat ggcggccaag gccgctggca tgagctatat ggatttgtgc gtcgaaattc
     1081 tggcgagcgc ctcgtgcaag gttcaggcgc cgctggaaat tgatcccgag gagcgaattt
     1141 aatcgtgttt aacgacgccc gcctgactaa ctttattgca aacatgctgg ctttgctggc
     1201 ggtgcttgcc atggtcgcgg gtgtcgttat atgggtagcg caacgtcctt attttgcgat
     1261 tacccaaatt caaatcgagc cgatgcgatc cggctcattg aactacgttt cggcaacgag
     1321 tgttcaggcc accatagcgg gccgattgat cggcaacttt ttcttcgtca agctcgatga
     1381 cgcgcggaag ttgattgaaa ctgtgccgtg ggtccgccac gcccgcattc agcgcgtctg
     1441 gccgaatacc ttgcgggttc agattgaaga gcagcaacca ttggcatact ggaacgacaa
     1501 tcagatgatc aattcctggg gcgaggcctt tagcgcgaac cagggcgagt tgcccgacga
     1561 tctggaactg ccgcatttga acgggccagc caattctgag cgcctggtag tgcaacggta
     1621 cgccgaattg gcgcgctggt ttgctccgtt ggccttaagc gttgcaaata tcacattaag
     1681 cccccgctat gcctgggagg tcgaactgtc ggatggcgtg cgtctgaacc tggggcgcga
     1741 ccccgccgcc gatgtggctg atccgcacgg ccgttccggc gccttgccat ttgccgcgcg
     1801 aatcgaacgt tttgtacaag aatggccgat attggcgcac cggcttggcg ggcgcgcaat
     1861 tagcagtgcc gatttacgct attccaacgg gtttgcgatt acgttggccc ctgttactac
     1921 cacaccttct aaatagaaaa attatgaccc gtgacatcaa ggatctgatt gttgctcttg
     1981 acatcggcac cagcaaagtg gtggcggtgg tggccgaaat attgccggaa ggccgcttcg
     2041 aagtgctggg cctgggtcag catgaatctc gcggcatgcg aaaaggtgtc gtggtcaata
     2101 tcgaagcaac cgtgaattcg attcagcgcg cgctcgaaga ggccgagttg atggccgatt
     2161 gcaaaattcg cgaagtctat acggggatcg ccggcagtca tatcaccagc tttaactcca
     2221 gcggcatggt cgcggttaaa gacaaagagg tgaccgccac cgatgtggcg cgcgtgattg
     2281 aaacggccaa ggcggtgaat attcccaccg atcagcaggt gctgcatgtg ctgacgcaag
     2341 aatttatcgt cgattcgcag gaagacatcc gcgagccgat aggcatgagc ggtttgcgcc
     2401 tggaagtgcg ggtgcatatc gttacgggct cggtcagcgc tgctcaaaat atcgtcaagt
     2461 gcgtgcgccg ttgcgggctt gaggtccagg atctgatctt gcagccgctg gcctccagtc
     2521 tggcgtgcct gacgtcggat gaaaaagaac tgggagtggt gctggtcgat ataggcgggg
     2581 gcacgacgga cattgccatt ta
//
LOCUS       DATCXU010000362         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_401675,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000362 DATCXU010000000
VERSION     DATCXU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401675"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VIR04_08150"
     CDS             complement(<1..958)
                     /locus_tag="VIR04_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY9460756.1"
                     /translation="MTSVASPTPPSNFLRTIIDNDLANNRYADKRWAGRPGPADIQEQ
                     GNIDTARIRTRFPPEPNGYLHIGHAKSICLNFGLALDYDGACHLRFDDTNPEKEEDEY
                     VKAIIDTVQWLGFDWRHNDESNCYFASNYFETMYQFAEALIAAGHAYVDAQSAEEMRH
                     ARGTLTEKGVNSPWRDRPSAESLALLREMRAGQHPDGSLALRAKIDMSSPNINLRDPV
                     LYRIRHAAHHRTGTDWCIYPMYSFAHPVEDALEGITHSICTLEFEDQRPFYDWVLSTL
                     ADLGQLRRPLPHQYEFARLNLSYVVTSKRKLLQLVQEGHVNGW"
     gene            1070..>2119
                     /locus_tag="VIR04_08155"
     CDS             1070..>2119
                     /locus_tag="VIR04_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY9460757.1"
                     /translation="MINSDLWLPQIQFSISLGFMSLFLVIELGLAWVLLFFKIRAAQS
                     GQVAWTIAYRFWVRVFALAFVLTFASSMPVLIQFGSLWPSLMDKIGEVAGPLLAATIL
                     TTFIFKSCFLGAMLFGQRRLSERVHTLVVFMVALGATGAAFWMVALQSWMQTPSGVDL
                     IDGQYRVVDWIAVVFNPSMAWYGGLLVLTSALTIAFLMLGVTARQTFRHPLDESGCLV
                     FKTGLIIAMVSVLLYGAIGIGAGQMMAKYQPAKAAATAAYWHSGVQPGIVLFGWPDAA
                     TQKNRGALEWAHAGGRWLGKDDQGRYRGLDQFSRMSPPVALTFWSFRSVVLVATLMAA
                     LSWLTFFKVRKKGFDP"
BASE COUNT          383 a          535 c          659 g          542 t
ORIGIN      
        1 cccaaccgtt tacgtggcct tcctgcacga gctgcaatag tttgcgtttg ctggtgacga
       61 cgtagctcag gttcagacgc gcaaattcat attgatgggg caggggacgc cttagctgcc
      121 ccagatcggc caatgtgctc aggacccaat cgtaaaaagg ccgttgatcc tcgaattcga
      181 gcgtgcagat gctgtgcgtg atgccttcca gggcgtcttc gaccggatgc gcaaatgaat
      241 acatcgggta aatgcaccag tccgtgccgg tgcggtggtg cgccgcgtgc cgaatgcgat
      301 ataaaacggg gtcgcgcaaa ttgatattgg gtgaactcat gtcgattttg gcacgcagag
      361 ccaggctgcc gtcgggatgt tgtcctgcgc gcatttcgcg cagcaacgcc agggattcgg
      421 cactgggccg gtcgcgccag ggcgagttga cccctttttc cgtcaaggtg ccgcgggcgt
      481 ggcgcatttc ctcggcgctt tgcgcgtcaa cataggcatg accggcggcg atcagggcct
      541 cggcgaattg atacatggtt tcgaagtaat tactggcaaa ataacagttc gattcatcgt
      601 tgtggcgcca gtcgaaaccc agccactgaa cggtgtcgat aattgccttg acgtactcgt
      661 cctcttcctt ttcgggattg gtgtcgtcga agcgcagatg gcacgcaccg tcgtaatcaa
      721 gcgccaggcc gaaattcaga cagatgctct tggcgtggcc gatatgcaga tagccattgg
      781 gttcgggcgg gaaacgtgtg cggatgcggg cggtgtcaat gttgccttgt tcctgtatgt
      841 cagccggtcc tggccgtccc gcccagcgct tgtcggcata gcggttgttc gccaggtcgt
      901 tgtcaatgat ggtgcgcaag aagttggaag gaggagtcgg gctggctaca gaggtcatac
      961 ttggctaagc gggaaaagtt ggatgaagtg tgcattttgc cacgttccgc ttaatttgct
     1021 tgtatcgggc agttataaag gaggcactcg ctttaaacta gcgcacatta tgatcaactc
     1081 agatctctgg ctaccgcaga ttcagttttc catcagtctg ggcttcatgt cgctctttct
     1141 ggtgattgag ctgggcctgg cgtgggtgct gctgttcttc aaaattcgcg cggcgcagtc
     1201 gggccaggtg gcatggacga ttgcctatcg gttctgggtg cgggtttttg cgctggcgtt
     1261 tgttttgacg tttgcgtcca gcatgcctgt cctgattcag tttggcagct tgtggccatc
     1321 cttgatggac aagatcggcg aggtcgccgg cccattgctg gccgcgacga ttttaaccac
     1381 attcatcttc aaatcctgct ttttgggagc catgttattc gggcaaaggc gtttgtccga
     1441 gcgtgttcac actttggtcg tgttcatggt tgcgctgggc gcgaccggcg ccgcgttctg
     1501 gatggtcgcg ctgcaatcct ggatgcagac tccttcggga gtggatctga tcgatggtca
     1561 gtaccgggtt gtcgactgga tagccgtggt gtttaatccg tcgatggcgt ggtatggcgg
     1621 cttgctggtc ttgacgtcgg ccttgaccat tgctttcctg atgctggggg tcaccgcccg
     1681 tcagaccttt cgccatcctc tggatgaaag tggctgcctg gtctttaaaa cagggctgat
     1741 cattgccatg gtgagcgttt tgctgtatgg cgcgatcggg atcggcgctg gacaaatgat
     1801 ggccaaatac cagcccgcca aggcggcggc cacggcagcg tattggcatt caggcgtcca
     1861 gcctggcatt gtattgtttg gatggcccga tgcggcaacc caaaagaatc gcggcgcgct
     1921 cgaatgggct catgcgggcg gacgctggct gggcaaggac gaccagggac ggtatcgcgg
     1981 actggaccag ttctcgcgca tgagtcctcc tgtcgcgctt accttttggt catttcgatc
     2041 tgtggtttta gtggcaacgc ttatggcggc tcttagctgg cttacatttt tcaaagtacg
     2101 aaaaaagggc tttgatcct
//
LOCUS       DATCXU010000363         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_113202,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000363 DATCXU010000000
VERSION     DATCXU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113202"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VIR04_08160"
     CDS             complement(<1..816)
                     /locus_tag="VIR04_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEY9460758.1"
                     /translation="MHIGIPRESRAGETRVAATPDTVKKYIAGGHTVTVEQDAGLAAH
                     FTNQTYAQAGATIGNAIQAFSADIILKVRAPSEAERRQIKPKAILVGMLDPFDQEGLA
                     ALASAGLTAFALEAAPRITRAQSLDVLSSQANLAGYKAVLLAANQYGRLFPMMMTAAG
                     TLKAARTVVLGTGVAGLQAIATARRLGSAVEASDVRPAAKEQVESLGAKFIDVPFETD
                     EERQIAEGVGGYARPMPAAWMARQAVLVSKRCEQADIVISTALIPGRPAPILIT"
     gene            1089..>2676
                     /locus_tag="VIR04_08165"
     CDS             1089..>2676
                     /locus_tag="VIR04_08165"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY9460759.1"
                     /translation="MKVWFKRISFGIVVAVIVALVGLVIFLLTFDPNAYKSKLEEIVY
                     NRYHRTLAIEGDIHLSLFPRIGLSVQNVSLSDRDSRSTFASVDSARFAVAIWPMLSNR
                     LVVDHVAVTGFKAWVVRDKLGVFNFHDLTESRSPWLDLPVVGDSGLSPVNAPKVADSP
                     SKPATLPAVLKNHEAARADFQIDIAGLDLKNGEIHLYDAVTGSVARIEKLVVNTGRMT
                     FDQPFDVTVKGRLFGEHPIADANIEGQALVKMSPEQKNYSAQKLNVQMTGRLGPLQAK
                     TASLKGNLAYSAYSRMFGASNLELLVQGGVDGAEPIKNLDANLSVRQLKADQSRAELN
                     LDKLSLRAKGDLPDQNFDVAFDAPSIAVSPDSAKSDVVSGTVKLAGPSVLGVTLGLRG
                     LDGNAQSLTLKELKVEGSLKQGDRLIQLNASSPASWDTVEDKGGLTAIKGDVKIEAPA
                     MRKGSFEFPLIGSMHADLKKDQLTSEINAVLNGSKLDFTVAATHLRDPNIMFNLDADS
                     LDFNRLFPPTEVKAPAPADAK"
BASE COUNT          562 a          699 c          785 g          630 t
ORIGIN      
        1 ggtgatcaga atgggcgccg gccggcctgg tataagcgcg gtgctaatga cgatgtccgc
       61 ctgctcgcag cgtttcgaca ccagtacggc ctggcgcgcc atccaggcag cgggcattgg
      121 ccttgcgtag ccgccgacgc cttcggcgat ctgccgctct tcgtcggttt caaatggcac
      181 atcgataaat ttcgcaccga gcgattccac ttgttctttg gccgcgggcc ggacgtcgga
      241 tgcttcgacg gcggagccaa gccgcctggc ggtggcaatg gcctgcaacc ccgctacccc
      301 ggtacccagc acaacggtgc gcgctgcttt aagcgtgcca gccgcggtca tcatcatggg
      361 gaacaggcga ccgtactggt ttgcggccaa cagcaccgct ttgtagcctg ccagattggc
      421 ctgtgaagat agcacgtcca gactttgcgc gcgtgtgatg cgaggcgccg cctcgagcgc
      481 aaatgccgtc aatccggcgc tggccagcgc ggcgagcccc tcttgatcga atggatcaag
      541 catgcctact aaaatggctt tgggttttat ttgccggcgt tcggcttcgc tgggtgcgcg
      601 aacctttaaa ataatgtcgg cggagaaggc ctggatggca ttgccgatgg tggcgcccgc
      661 ctgcgcgtag gtctgattcg taaaatgggc ggccaggccg gcgtcttgtt cgaccgtgac
      721 ggtgtgaccg cccgcaatgt atttttttac tgtgtcgggc gttgcggcaa cgcgtgtttc
      781 accagcccgg ctttcgcgtg gaattcctat gtgcatagtt tgtggctcgc cttccatcgt
      841 tacttttaac ggtggttact ataaccgaac ttgtttacat cctgtttaca ttttcggcga
      901 gcaacccatc ggccgatttt gtttgcaatt gtgtgcggcc atccgcatac atagccagtt
      961 atgacatctt gtaaggcttt taccggttaa atggcggaag tctgaacatc gggcacgagt
     1021 taggttagca ttccatctat tgacattgtg tggcaccagg gaaactgccc tggcttttga
     1081 ggcgggaaat gaaagtttgg tttaaacgga tttcttttgg catagtagtt gcggtaattg
     1141 tggcattggt ggggcttgtg attttcttgt tgacattcga tccgaatgcc tacaaaagca
     1201 agctggaaga aatcgtatac aaccgttatc accgcacgct ggcgatagag ggtgatattc
     1261 atttgtccct gtttccgcgt attggccttt cggttcagaa cgtgtcgcta tcggaccgcg
     1321 acagccgcag cacatttgcg tcggtggaca gcgcgcgatt tgccgtggcc atctggccta
     1381 tgctgtccaa ccgtctggtg gtcgatcacg tggcggttac gggattcaag gcgtgggtcg
     1441 ttcgcgataa gctgggcgta ttcaattttc atgatttgac tgaaagccgc tcaccctggc
     1501 tggatctccc tgtggtgggc gactccggat tgagccccgt taacgcgcca aaggtcgccg
     1561 attcgccttc caagccagcg acattgccgg ccgtgctgaa aaatcacgaa gccgcgcgcg
     1621 cggattttca gatcgacatt gccggtctgg atttgaagaa cggagagatt catctttacg
     1681 atgcggtgac gggctcggtt gcgcgtattg aaaaactggt tgtgaatacg ggccggatga
     1741 cgttcgatca accattcgat gtcaccgtga aaggccgtct gttcggcgag cacccgattg
     1801 cagatgccaa tatagaaggt caggcgctgg tcaagatgag cccggagcaa aaaaactatt
     1861 ccgcgcaaaa attgaacgtt caaatgacag ggcgtctggg gccgttgcag gccaagactg
     1921 cctcgttaaa aggcaatctg gcctatagcg cttattcccg aatgtttggt gcaagcaatc
     1981 tggaattgct tgtgcaaggc ggtgtcgatg gcgccgagcc catcaagaat ctggacgcca
     2041 acttatccgt gcggcaacta aaagccgacc agagccgggc cgaactcaat cttgacaagc
     2101 tgtctttgcg cgccaaaggg gacctgcctg atcaaaattt tgacgtcgcg ttcgacgcgc
     2161 ccagcattgc tgtctcgccg gattcggcca agagcgacgt cgtctccggc acggtcaagc
     2221 ttgcgggtcc aagcgtgctg ggcgtgacgc tgggtttgcg cggtcttgac ggcaacgcgc
     2281 aaagcctgac tctgaaagag ttgaaagtgg agggtagcct gaaacaaggc gatcgcctga
     2341 tccagttgaa cgcgtcatcg ccggcaagct gggacactgt cgaggacaaa ggcggcctga
     2401 cggccatcaa gggcgacgta aaaattgaag cgcccgccat gcgcaagggc agtttcgagt
     2461 tcccgctgat tggaagcatg cacgctgatt tgaaaaaaga tcagcttaca agtgaaatca
     2521 atgccgtatt gaatggcagc aaacttgatt tcactgtagc ggcgacccat ctgcgcgatc
     2581 caaatatcat gtttaatctg gatgcggatt cgctggactt taatagattg tttccgccta
     2641 cagaagtcaa agcgcctgcg cctgcggatg ccaaaa
//
LOCUS       DATCXU010000364         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_678039,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000364 DATCXU010000000
VERSION     DATCXU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678039"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VIR04_08170"
     CDS             complement(<1..1592)
                     /locus_tag="VIR04_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEY9460760.1"
                     /translation="MKTEPILYSLTPFDPAGHRFMLRLTIRTPDPAGQELTLPAWIPG
                     SYLIRDFSRQIETIHARHNKQSIPIEKTGNHSWRCAPCAGPLIIEYVVYAWDLSVRGA
                     HLDESHAFFNGSSVFLCPSGFEDNPCHVEFCRPAHGRDWKVYTSLPQAQGLKQPAKRH
                     GFGVYAAPNYDALIDFPVEMGTPQTVRFNVHGAEHEIVFTGVIPNLDTKRIAADTKKI
                     CASQIELFEPISKKAPFLDSSNRYVFLTTITGDGYGGLEHRASCALIASRHDLPTIGN
                     EPAEGYQTFLGLISHEYFHTWNVKRIKPAAFAPYDLSRENHTRLLWVFEGFTSYYDDL
                     MLLRSGTIARPDYLRLLGKAVSNVRRATGRRKQTIAESSFDAWTRYYKQDENSPNALV
                     SYYTKGSLVALGLDLTIRQATANARSLDDVMRLLWVRYGRDFYSLHKTGVPENAVTAL
                     IHEATGVDVSEFIKRHVDGYEDVPLEGLLAGQGLTIKWKAAGQAPSLDARTRVTQGEV
                     QLAAVYEGGAAHQAGLSAGDSLV"
     gene            1623..2396
                     /locus_tag="VIR04_08175"
     CDS             1623..2396
                     /locus_tag="VIR04_08175"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY9460761.1"
                     /translation="MNAPLVKVETRGRVGWLTLNRPKVLNALNDALIDELKTALQGFD
                     ADDAIGAIVLTGSEKAFAAGADIGAMKNWSYMDVYRGDYLATWDSLKNIRKPIIAAVA
                     GYALGGGCELAMLCDFIIAAENARFGQPEIKLGVIPGAGGTQRLPRAISKAKAMDLVL
                     TARLMDATEAERAGLVSRVVPLERLLDEALDAATTIASMSLPSVLMAKECVNRAYESP
                     LNESLMFERRNFHALFATEDQKEGMHAFAEKRKPAFKNR"
     gene            2507..2923
                     /locus_tag="VIR04_08180"
     CDS             2507..2923
                     /locus_tag="VIR04_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY9460762.1"
                     /translation="MEPKPELSAATLAALDATAGRGIIRALAAQAVMGLLAGLISWIA
                     AGFVAGASALIGAGAYLVPNALFALRLLAGLWGRQKVNPLAFFLGEAFKLGSAMLLLG
                     LATYYGREWLVWPAFLFGLLCALKGYVLLLVFHKLP"
     gene            2984..3865
                     /gene="atpB"
                     /locus_tag="VIR04_08185"
     CDS             2984..3865
                     /gene="atpB"
                     /locus_tag="VIR04_08185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY9460763.1"
                     /translation="MAAASEISPQSEYIQHHLVHLNSSGVNQADIINFDIVNYDSVFW
                     AVLTGVVVVYVLWRAAKSATNAVPGRFQMAVEMLVGWVEDEAKGIISNEVSRRFMAPL
                     ALTIFLWISLMNALDLVPVDFLSQIFRATGLGASHDDILYYHRILPTADLNVPMGMAL
                     GVLVLTLYYGVKAKNPVGFVKELLVAPFHAHGIAGLLLTPFNLLLNIIEYVAKTVSLA
                     LRLFGNMFAGELIFLLVALLGGAWTGFNGMSLSLGLGHIIAGSVWAIFHVLIVLLQAY
                     IFMMLALVYVGLSQESH"
     gene            3923..4159
                     /gene="atpE"
                     /locus_tag="VIR04_08190"
     CDS             3923..4159
                     /gene="atpE"
                     /locus_tag="VIR04_08190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY9460764.1"
                     /translation="MTNVAFVALACGLIIGLGALGACVGIGVMGSKYLEASARQPELM
                     NALQTKMFLLIGLIDAAYLIGVGIAMLFAFASPF"
     gene            4286..>4412
                     /locus_tag="VIR04_08195"
     CDS             4286..>4412
                     /locus_tag="VIR04_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY9460765.1"
                     /translation="MNLNATLFFQMIVFFVLGWFTMKFVWPPLTKAMDDRRQKIAD"
BASE COUNT          868 a         1089 c         1313 g         1142 t
ORIGIN      
        1 acaagactat cgcccgccga cagtcctgcc tggtgtgcgg ctccgccttc gtacacggcg
       61 gcaagctgca cttcgccttg cgtcacgcgc gtgcgtgcgt ccagcgatgg cgcctgccca
      121 gcggctttcc atttgatcgt caggccttgt cctgccagca acccttcaag cggcacgtct
      181 tcgtatccgt cgacgtggcg cttgatgaat tcggacacat ccacgccggt cgcttcgtga
      241 ataagcgcgg tcacggcatt ttcgggtacc cccgtcttgt gcaatgaata gaagtcgcgc
      301 ccgtaccgca cccacaataa acgcatgaca tcgtccagtg acctggcgtt ggctgtggcc
      361 tgacgtattg tcaggtccag gcccaatgcg accagcgacc ccttggtgta gtagctaacc
      421 agcgcattgg gcgaattttc atcctgcttg taataccgcg tccaggcgtc gaacgaactt
      481 tcagcaatgg tttgttttcg ccgccccgtt gcgcggcgca cgttgctgac cgctttgccc
      541 aggagtcgca ggtaatcggg gcgtgcaatc gtacccgaac gcaatagcat caagtcgtcg
      601 taatacgagg tgaagccttc aaatacccac agcagccgcg tgtggttttc acggcttagg
      661 tcgtaagggg caaaagccgc cggcttgatt cgcttgacgt tccaagtatg gaaatactca
      721 tggctgatca gccccaggaa agtctggtag ccttcagcgg gttcgttccc gatggtgggc
      781 agatcatggc gcgatgcgat cagcgcgcac gacgctcggt gctcgaggcc gccatagccg
      841 tcgcccgtaa tggtggtcaa gaatacgtag cggttggaag agtcaagaaa cggcgctttt
      901 ttactgattg gttcgaacag ttcgatctga gacgcgcata ttttttttgt atcggcagcg
      961 attcgctttg tatccagatt gggaatgacg ccggtaaaga ctatttcgtg ctccgcgccg
     1021 tgtacgttaa agcgaacggt ttgcggcgtg cccatttcca ccggaaagtc aatcagcgca
     1081 tcgtaattgg gcgcggcata aaccccgaag ccatgacgtt ttgcgggttg tttgagccct
     1141 tgagcttgtg gcagactggt gtagaccttc cagtcgcggc catgcgctgg gcggcagaac
     1201 tcgacgtggc acgggttgtc ttcaaagccg cttgggcaca agaaaacgct cgagccatta
     1261 aaaaaggcat gcgattcatc cagatgcgcg ccgcgcacgg aaaggtccca cgcatatacg
     1321 acatattcaa taatcagggg gcccgcgcat ggggcgcagc gccaggaatg attccctgtt
     1381 ttttctattg ggattgattg tttgttgtgc cgggcgtgga tcgtttcgat ctggcgcgag
     1441 aaatcacgta tcaggtagct gccgggaatc caggcgggca atgtcagttc ttgtccggct
     1501 gggtcgggtg tgcggattgt cagccgcagc ataaatcggt ggccggcggg atcaaatggc
     1561 gttaggctgt aaagtatggg ttctgttttc attccactat attactgtgc gacggaggca
     1621 acatgaatgc gccattggta aaagtcgaaa ctcgcggccg ggtgggttgg ctgacgctta
     1681 atcgtcccaa ggtcctgaat gcacttaacg atgcattgat tgatgaactg aaaacggcac
     1741 tgcaagggtt tgacgccgat gacgctatcg gcgccatcgt attgaccggc agcgaaaagg
     1801 cctttgcggc cggcgctgat atcggcgcca tgaaaaattg gtcatatatg gacgtgtatc
     1861 gcggcgatta tctggcgaca tgggattcgc tgaagaatat tcgcaagccg attattgccg
     1921 cggtggcggg gtatgcattg ggcggcggct gtgaactcgc catgctgtgt gattttatta
     1981 tcgctgccga aaatgcgcgc ttcgggcagc ccgaaatcaa gctgggcgtt atacccgggg
     2041 cgggcgggac tcagcgcttg ccacgggcca tcagcaaggc caaggcgatg gatctggttc
     2101 ttacggcccg tctgatggac gccactgaag ccgagcgcgc tggcttggta tcccgggtag
     2161 tgcctctgga gcgtttgctt gatgaggcgc ttgatgcggc caccaccatc gcgtcgatgt
     2221 ccctgccttc tgtattgatg gccaaagaat gcgtcaatcg cgcttacgag tcgcccttga
     2281 acgaatcgct aatgttcgag cgccgcaatt ttcacgcttt gtttgcgact gaggatcaaa
     2341 aagaaggaat gcacgcattc gccgagaagc gcaagcctgc gtttaaaaac cgttaggcca
     2401 acaccgtgcg cctgctgaca atttgcttac tgtgaaaaaa taaagttata ctcttacggt
     2461 ttgacgctgg tagtgcaaac acgtgaaggc caagacggat agccagttgg aacctaagcc
     2521 agaactgagc gcggctacgc tggctgcttt ggacgccacg gccggcaggg gtataatccg
     2581 agccctggca gcccaggctg ttatgggttt attggctgga ttgatttcct ggattgcggc
     2641 aggttttgtt gcaggggctt ccgccctgat aggggcgggg gcttatcttg tgcccaatgc
     2701 actgttcgcg cttcgattgt tggcgggcct gtggggccgc caaaaggtga acccgctggc
     2761 tttctttctg ggcgaagcgt tcaagcttgg ctcggcgatg ttgctgctgg gtttggccac
     2821 gtactatggg cgcgagtggc tggtgtggcc ggccttcttg tttggtttgt tatgcgcgtt
     2881 gaagggctat gtattgttgc ttgtgtttca caagctgccg tagcgattgg tttaaattca
     2941 tagggcttgc gctcgagcgc gggttaacga gataagggtt acgatggctg ccgctagtga
     3001 aatttcgcca cagtccgagt acatacagca ccacctggtt catctaaaca gttcgggcgt
     3061 taatcaggct gatatcatca acttcgacat tgttaattac gattcggtgt tctgggcggt
     3121 attaacagga gtggttgtag tctacgtttt gtggcgcgcg gcaaaatccg ccacgaacgc
     3181 tgtgccgggc cgctttcaga tggctgtgga aatgctggtt ggctgggtcg aagacgaggc
     3241 gaaaggcatt atttccaacg aagtttcgcg ccgcttcatg gcgccgctgg cgctgaccat
     3301 attcttgtgg atctcgctga tgaacgcgct ggacctggtg ccggtcgatt ttctgagcca
     3361 gatattccgg gctaccggcc tgggcgcgtc gcatgacgat atcctttatt accatcgcat
     3421 attgccaacc gccgatttga acgtgcccat gggcatggct ctgggcgtgc tggttctgac
     3481 tttgtactac ggtgtcaagg caaagaatcc tgtcgggttc gtcaaagagc tgctggtggc
     3541 gccgtttcac gcacatggca tcgcgggctt gctgcttacg ccgttcaatc ttttattgaa
     3601 cattatcgaa tatgtcgcca agaccgtgtc tctggccttg cggttattcg gcaatatgtt
     3661 tgcgggcgag ctcattttcc tgctggtcgc cttattgggc ggggcatgga caggatttaa
     3721 tggcatgagc ctcagtttgg gcttgggcca tattattgca ggatcggtgt gggccatatt
     3781 ccacgtcctg attgtattgt tgcaggcgta cattttcatg atgctggccc tggtgtatgt
     3841 gggtctgtct caggaaagtc attagtaaat aggtactctt ctttaacttt ttgattacaa
     3901 ggtttttaac caaggagttc tcatgaccaa cgttgctttc gttgctcttg cttgcggtct
     3961 tattattggt ctcggtgctt taggggcttg cgttggtatt ggtgttatgg ggagcaaata
     4021 tctggaagct tccgctcgtc agcctgagct gatgaatgcg ctgcagacca agatgttcct
     4081 gctgattggc ttgatcgatg cggcttacct gatcggtgtc ggtattgcga tgctgttcgc
     4141 tttcgccagc ccgttttaag tgactatgcc cgcgtttggc gggttggatt gccgttacgg
     4201 tgcaataggt actgttggcg gcgtggacat caggtgttat gcgggtaaaa ctgcttggcg
     4261 cccgtcgtat taaagggaaa cgaacgtgaa tttaaacgcg actctctttt ttcagatgat
     4321 cgtgtttttc gttttaggct ggtttacgat gaaattcgta tggccgcctt tgacgaaggc
     4381 aatggacgat cgccgccaga aaattgccga cg
//
LOCUS       DATCXU010000365         4116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_663808,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000365 DATCXU010000000
VERSION     DATCXU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663808"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VIR04_08200"
     CDS             <1..802
                     /locus_tag="VIR04_08200"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247444.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEY9460766.1"
                     /translation="MMKKLSNSAACVFTGKGVAFGGSLIRPEATGYGAVYFAEEMLKH
                     TGQSIEGLKVSVSGSGNVAQYAIEKCMQLGARVVTVSDSHGAVIDKDGFTPEKLAALI
                     ELKTKKRGEIETYAKQFHLTYEAGKRPWHVPVDVAMPCATQNELELEDAQTLIKNGVR
                     CIAEGANMPTSLDAVQAFIDAKVLYAPGKASNAGGVAVSGLEMSQNSQRLQWTREQVD
                     NKLHEIMREIHEHCVVHGSDGKWVNYVNGANIAGFVKVADAMLQQGVY"
     gene            complement(799..4089)
                     /locus_tag="VIR04_08205"
     CDS             complement(799..4089)
                     /locus_tag="VIR04_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEY9460767.1"
                     /translation="MSDSVFKWLASQRQILLRNATIRWVDARRRAPPLVLKNVTLKAD
                     NQGDQHVFSLAATPPAELGETFDLRGAFQSAALAESPEFALQSWNGQLYVRVDGMSPT
                     AWKPWVDIPQNLDSGQVSAQAWLGIEQGRASRFTSDVSIQNAHWTFGPENSVRAGSVR
                     LYLDGPRAAFERLFSDPAAVADAQRPVDDAPAAPLDPARVVDSGAPANTQFLFTAHAL
                     SVQVAEIFNHPLNFDEVSAQGALDRQQNRALSFAFSHAHLLNRDMDAQLAGTWRQGGS
                     GVAGIADIRGVFERAAVSEIDEYLPTMVDLDARDWMARGLTGGQIHDARLVLRGDLEH
                     FPFVDNPEAGDFSVRGDYTGGVIDYLPAQGKRLGWPQLSDMSGKVSLHRADLRIVADR
                     ARMWPTKESAIELSDVRAQIPDIEHESVLTIAGKTTAPADTYLALMRHSPLGGLLDNA
                     FDKAKASGDWQVPLRLTIPLSHGNDTAVDGLIRFADSSVALTPEIPVFSHVNGILNFS
                     DIALNVSDLKASFLGGPIAFSGGVGGQYQGLKMQGQANTAALGEYVAPAGVKRLSGKL
                     KYRATLLREKSKRYSLTLESDLVGVGINLPEPLSKKEEQAWPLRLKWVPEGKDAVVLN
                     IDLADKLNVALLRDRAAHSAAYFQAGTVGVNQAPPLLRSGLNVDIRYPTVDIDLWNQV
                     IDDFSTPQAKAATARRQPLLPDLHEFRLQAGYVQLQGMEMDHVIFTAKQPQPLQWRVD
                     ISSSQTAGTLFWREAQGKVAGNIEAKFDRLALGHNAENTNSVPDSSTPDNDISRVHDY
                     FDMPAIDLKVDKFTLYGHELGSLSVQGVNQQRGELWKLNKLNLVSPSSHIKGSGLWRL
                     NGLDRGLTLDAQADISNLGDYSDQLGYKNVLQQGSGTVSGQVVWHNLPWAYKKTDLSG
                     RIEVDFKKGRFNNVGSRSARLLKLLSLQSVKRLAAFKFDPASLLKDGFPFDNLRGTIL
                     VNKGIVTTDNYRVVGPLGTVVIGGNVNLSSEQLNLQAVVVPNLDVSGAAIAAGIAINP
                     IVGIGAFLTQWLLQAPLAKAMTAQYQISGNWDDPKVKEVAHVQETGKAQPAQAGKSAL
                     PLAAELKR"
BASE COUNT          927 a         1242 c         1079 g          868 t
ORIGIN      
        1 aatgatgaaa aaactgtcca attcggccgc atgcgtgttt accggaaaag gtgtggcctt
       61 tggcggcagc ctgattcgcc ccgaggccac aggttatggc gccgtctact tcgctgaaga
      121 aatgcttaag cacaccgggc aatcaatcga aggtttgaag gtttcagtat ccggctcagg
      181 caacgtggcg caatacgcca tcgaaaaatg catgcagctt ggcgcccggg tcgttaccgt
      241 ttcagattct catggcgctg tcatcgacaa agacgggttt actcccgaaa aactggcggc
      301 gctgatcgag cttaaaacca agaagcgcgg cgaaatcgaa acctacgcca agcagttcca
      361 cctgacctac gaagccggaa aacgcccatg gcatgtgccc gtcgacgttg ccatgccctg
      421 cgccacgcaa aacgaacttg aactcgagga tgcgcaaacc cttattaaga acggcgtccg
      481 ttgcatcgcc gaaggcgcaa acatgcccac cagtcttgac gccgtccagg cgtttattga
      541 cgctaaagtg ctttatgcgc ccgggaaagc aagcaacgct ggcggcgtag cggtatccgg
      601 actcgaaatg agccagaact cgcaacgctt gcagtggacc cgcgagcaag tcgacaacaa
      661 gctgcatgaa atcatgcgtg aaattcacga acattgcgtc gttcacggca gcgacggcaa
      721 gtgggtaaat tacgtcaatg gcgccaatat tgccggcttc gtcaaagtgg ccgacgccat
      781 gctgcaacag ggcgtttatt agcgcttaag ctcagccgcc aggggcaagg ctgacttccc
      841 ggcttgcgcc ggttgcgcct tgcctgtttc ctggacgtgg gccacttctt taaccttggg
      901 atcgtcccaa ttgccgctga tttgatactg ggcggtcatg gccttggcca gtggcgcctg
      961 taatagccat tgcgtcaaaa atgcgccaat acccacaatt ggattgattg caatgccggc
     1021 cgcaatcgcc gcgccgctta catcaagatt gggaacgact accgcttgca gattcaattg
     1081 ttcgctcgac aggttcacat ttcctccgat aaccacagtg cccaatggac ccaccacacg
     1141 gtagttatcg gtagtgacga ttcccttgtt cacaagaatc gtgccgcgca aattgtcgaa
     1201 ggggaagccg tctttaagca ggctggccgg atcgaattta aacgcggcca ggcgcttgac
     1261 tgactgcagc gacaaaagct tgagcaaacg cgccgaacgc gagccgacgt tattgaaacg
     1321 acctttcttg aaatcgacct caatgcgccc gctcaggtcc gtcttcttgt aagcccaagg
     1381 taggttgtgc cacaccacct gtccgcttac cgttcccgaa ccttgctgca aaacgttttt
     1441 atatcccagt tgatcggaat aatcgcccag gttgctgatg tcagcctgag cgtccagggt
     1501 caaaccacga tcgagtccat ttaaacgcca taaccccgag cctttgatgt gcgaggaagg
     1561 gctaaccaga ttaagcttgt tcagcttcca aagttcaccg cgttgctggt taacaccttg
     1621 aaccgatagc gagcccaatt cgtgcccgta aagtgtaaat ttatccacct tcaggtcgat
     1681 cgcgggcatg tcgaaataat cgtgcaccct tgaaatatca ttatcggggg tgctggaatc
     1741 cggcaccgaa ttcgtgtttt cggcattatg tccgagcgcc agccgatcaa atttcgcctc
     1801 gatatttccc gctacttttc cctgcgcctc gcgccagaac aaggtgcccg ccgtttgcga
     1861 cgagcttata tcgactcgcc attgcaaggg ctgcggctgc ttggcggtga agattacatg
     1921 atccatctcc ataccctgaa gctggacata acccgcctgc aacctgaact catgtaaatc
     1981 gggcaatagc ggctgcctgc gtgccgtcgc cgccttggcc tgcggcgtgg aaaaatcgtc
     2041 gattacctga ttccacagat caatatcgac cgtaggatac cgaatgtcga cgttcaagcc
     2101 tgagcgcaat aaaggaggcg cctgattaac gcccaccgta cccgcctgaa agtaggctgc
     2161 cgagtgggcc gcgcggtcac gcagcaaggc cacgttcagc ttgtcggcca ggtcaatatt
     2221 caagaccacc gcatctttgc cttccggcac ccacttcaga cgcaagggcc aagcctgttc
     2281 ttccttcttg gacaacggtt caggcaaatt tattcccacc ccgaccaaat cggattccag
     2341 cgtcaacgaa taccttttag acttttcccg caacagcgtg gcgcggtact tcagttttcc
     2401 gcttaaccgc ttcacgccgg ccggcgccac atattcgcca agcgccgctg tgttcgcctg
     2461 gccttgcatt tttaaccctt ggtactgccc cccgaccccg ccgctgaaag caattggccc
     2521 acccagaaag ctggctttca agtcggacac atttagcgca atatcggaga aattcaggat
     2581 tccgttcacg tgactgaaca ccgggatttc gggtgtaagc gccactgaac tatctgcaaa
     2641 cctgatcaag ccgtctaccg cggtgtcatt gccatgcgaa agtggaatcg tcagccgcag
     2701 cggaacctgc caatcgcccg acgccttggc tttgtcaaag gcattgtcca aaagaccgcc
     2761 caaaggagaa tggcgcatca aagccagata tgtatcggcg ggcgccgtcg ttttcccggc
     2821 aatggtcaac accgactcat gttcgatatc cggaatttgg gctcggacgt cgctaagttc
     2881 gatcgcgctc tctttggtgg gccacattct ggcgcggtcc gccactatac gtaaatcggc
     2941 gcggtgcaaa gacaccttgc cgctcatgtc gctaagctgg ggccagccca gcctcttgcc
     3001 ttgtgcgggc aagtaatcga taacgccgcc tgtatagtcg ccccttacgc tgaaatcgcc
     3061 cgcttccgga ttatcaacga acggaaaatg ctccaggtcg ccgcgcagga caagccgtgc
     3121 gtcgtggatc tggccgccgg tcagccctct ggccatccag tcgcgcgcgt caagatcgac
     3181 catcgtaggc agatattcgt ctatttccga cacggcggcc cgctcgaaca caccacgaat
     3241 gtccgcaatg cccgccacgc cgcttccgcc ctggcgccac gttccggcca gttgtgcatc
     3301 catatcccga ttcagcagat gagcgtggct gaacgcgaaa ctaagagcgc gattctgctg
     3361 tcgatccaaa gcaccctgag cgcttacctc gtcaaaatta agcggatgat tgaaaatttc
     3421 ggcgacttgc acgctgagcg cgtgcgctgt aaacagaaac tgagtgttcg caggcgcccc
     3481 ggaatcaaca acacgcgccg ggtcaagcgg cgctgccggt gcatcatcca cgggccgttg
     3541 cgcatcggcg accgcagccg ggtcggaaaa taggcgctca aacgcagccc tgggcccatc
     3601 caggtaaagt ctgaccgaac cggcgcgcac gctgttttcc ggcccaaacg tccagtgcgc
     3661 gttctggatg ctcacgtccg aggtgaaacg cgatgcacgg ccttgttcta ttccaagcca
     3721 agcctgggcc gaaacctgcc ccgaatcaag attttgaggt atatccaccc agggcttcca
     3781 ggcggtgggc gacatgccgt cgacacgcac gtaaagctgc ccattccacg attgcaatgc
     3841 aaattcgggg gattcagcca acgccgccga ctggaatgcc ccccgaaggt cgaacgtttc
     3901 acccagttcc gccggaggcg tcgccgccag cgaaaagacg tgctgatcgc cttgattgtc
     3961 tgcttttaag gtaacgtttt ttagcaccag aggcggtgcg cggcgccggg catcgaccca
     4021 tcggatcgtg gcgttacgca gcaagatctg gcgctggctc gcgagccatt taaagaccga
     4081 atcgctcacg tcctgatcct gcgtcgccac atcgtc
//
LOCUS       DATCXU010000366         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_428182,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000366 DATCXU010000000
VERSION     DATCXU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428182"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VIR04_08210"
     CDS             <1..697
                     /locus_tag="VIR04_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460768.1"
                     /translation="ALIGSLLILPVLRTRSHYAALVTLAFGILFELFLEVNDTLGGPQ
                     GVRIPGMTLFGWPMNENIEIAGLEISFVVNYAVLSLILCLVAFTLVRRLERSWIGVSM
                     DIVRTDEITAATFGIPIARVKIIAFTVANFLIGAAGAVYGLLTSYTAPSNFTFADSLI
                     LVSIVLLGGIGNPWGILPAAAIILILPQKLQFIQEYRFLLYGLMVIAILLFRPDGLLP
                     RSVRKYFPGQCKL"
     gene            694..1476
                     /locus_tag="VIR04_08215"
     CDS             694..1476
                     /locus_tag="VIR04_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460769.1"
                     /translation="MSTPLLIARDLVVRFGGLMALDALNLTVYEGEVLGLLGPNGSGK
                     TTFFNVVTSLYTASSGQIMLAGENIMGKSPQQVYRRGVTRTFQRSRLSLPLTVFDNMV
                     IGNYQHLNLGLWFNLLQRKKFKAQYDQMTEQAYTLLHTFNPALAGKLFEPTGALTMID
                     RRRVEICRALLSNPRLVLLDEPSAGMTHDETNELMDDILQVRGLLKPFTIVIIEHEMN
                     VIQRITERCVVLNFGQKIAEGSYAQIVADPQVQIAYLGQEQE"
     gene            1473..2183
                     /locus_tag="VIR04_08220"
     CDS             1473..2183
                     /locus_tag="VIR04_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460770.1"
                     /translation="MTTLLKVEDIATAYDRIDVLHGISLEVEQGKITCILGSNGSGKS
                     TLIRSILGLTPPNKGRIEFEGISLLGLPTHKIIGRGISCIPEGRKVFPKMTVEENLRV
                     GAYQLSGESELKRRLAQVYEIFPRLKERTTQLAGTMSGGEQAMLSIGRGLMPNPKMLI
                     LDEPSLGLSPLFVKENFRVIRDINKSGMTILLVEQNARQTLAISDYGYVLSQGRLEAS
                     GSATELAENAQVRAAYFG"
     gene            2238..>2568
                     /locus_tag="VIR04_08225"
     CDS             2238..>2568
                     /locus_tag="VIR04_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY9460771.1"
                     /translation="MNQNSQASKTNTPIERLGAAQTARLLNRREISAEQVTRACLDRI
                     QEREPTVQAWTALRADGALAQARQLDRGPIQGLLHGLTLGIKDIFDTADLPTGCGSPI
                     YAGYQPRV"
BASE COUNT          590 a          737 c          671 g          570 t
ORIGIN      
        1 tgccctgatc ggatctttat taatactgcc ggtgctgcgc acccgcagcc actacgccgc
       61 actggtcacg cttgcctttg gtatcctttt cgaacttttt cttgaagtaa acgacactct
      121 tggcgggccg caaggagtgc gcattccggg catgacgctg ttcggctggc ccatgaacga
      181 aaacattgaa atcgccggtc tggaaatttc tttcgtcgtc aattacgctg tgctaagcct
      241 gatcctgtgt ctggttgcct tcacgctggt gcgcaggctt gaacgttcat ggatcggcgt
      301 atctatggat atcgtgcgca ccgacgaaat taccgctgcc actttcggca taccgattgc
      361 ccgcgtaaaa atcatcgcct ttacggtggc caattttcta atcggcgccg caggcgccgt
      421 ctatggcctg ctgacgagct acaccgcgcc cagcaatttc accttcgccg attcgctcat
      481 tctggtttcg atcgtgcttt tgggcggtat cggcaatccg tggggtattc tgcctgcggc
      541 agccattatc ctgatactgc cgcaaaaatt gcaattcatt caggaatacc gttttctcct
      601 ctatggacta atggtgatcg ccatcctgct atttcgccca gacggcctat tgccacgctc
      661 agtgcgcaag tactttccgg ggcaatgcaa attatgagca cgcctctttt aattgcccgc
      721 gatctggttg tgcgcttcgg cggattaatg gcgcttgacg ctctgaatct gacggtatac
      781 gaaggcgagg tgctgggact gctcggacct aatgggtcgg gcaaaaccac atttttcaac
      841 gttgtcacca gcctgtacac ggccagttcc ggccagatca tgctggcggg cgaaaacatc
      901 atgggaaaat cgccgcaaca ggtataccgg cgcggagtca cgcgcacctt tcagcgctcc
      961 cggctgtcat tgcccttaac cgtctttgac aatatggtca tcgggaacta tcagcatctg
     1021 aatctgggtt tgtggttcaa tttattgcaa cgaaaaaagt tcaaggcgca atacgaccag
     1081 atgacggaac aggcgtatac gctgttgcat acgttcaacc ccgcgttggc aggcaaatta
     1141 ttcgaaccga ccggcgccct gaccatgatt gatcgacgcc gggtcgaaat ctgccgtgcc
     1201 ctgcttagca accccaggct ggtgttgctg gacgaaccgt ctgcgggaat gacgcacgac
     1261 gaaaccaacg aacttatgga tgacatcctg caggtgcgcg gactgctgaa accattcacc
     1321 atcgtcatta tcgaacatga aatgaacgtc atccaacgca tcactgaacg ttgcgtggtg
     1381 cttaacttcg gccaaaaaat cgcggaaggc agctacgcac aaattgtcgc ggatccgcaa
     1441 gtgcaaatcg cctatctcgg gcaggagcag gaatgaccac gcttcttaaa gtcgaggata
     1501 tcgccactgc ctatgatcgc atcgatgtcc tgcacggcat ttcacttgaa gtggaacagg
     1561 gaaaaataac ctgcattctg ggatccaacg gatcggggaa gtccacgctt atacgttcca
     1621 ttcttggcct gacgccgccc aacaaggggc gtattgagtt cgaaggaatt tctctgcttg
     1681 ggttgccgac ccacaagatc atcggacgcg gaatctcgtg catcccggag ggccgcaagg
     1741 tttttcccaa aatgacggtc gaggaaaacc ttcgtgtcgg cgcgtatcaa ctttccggtg
     1801 aatccgaact taagcgccgg cttgctcaag tctacgaaat cttcccacga ctgaaagaac
     1861 gcaccaccca gttggctggc acgatgtcgg ggggcgagca agccatgctc tccatcggac
     1921 gcggcctcat gcccaatcct aaaatgctga ttctcgacga accgtcgctg ggcttgtccc
     1981 cactgttcgt caaagaaaac tttcgcgtca tccgcgacat caacaaatcg ggaatgacga
     2041 tattgctggt cgagcaaaat gcccgccaga ctttggccat ttccgactac ggctacgtcc
     2101 tttcgcaagg acggctcgaa gccagcggaa gcgccacgga actggctgaa aacgcccagg
     2161 tgcgagcggc ctatttcggt taggcaataa tggaatactg gccgacgcgc ccaaaaaacc
     2221 aatagttgga gtattaaatg aatcagaatt cccaggcttc aaaaacaaac acgccgattg
     2281 aacgccttgg cgccgcacaa accgcgcgac tgctcaaccg cagggaaata agcgccgagc
     2341 aagtcacgcg ggcttgcctg gaccgcatac aggagcgcga accaaccgtg caggcatgga
     2401 ccgcattacg ggccgacggt gccctcgcac aagcgcggca gcttgatcgc ggccccatcc
     2461 agggcttgct gcacggcctg acgctgggca ttaaagacat ctttgacacg gcggatctgc
     2521 ctaccggatg cggctcgccg atctatgccg gctaccagcc ccgtgtcg
//
LOCUS       DATCXU010000367         4459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_205602,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000367 DATCXU010000000
VERSION     DATCXU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205602"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..793)
                     /gene="maiA"
                     /locus_tag="VIR04_08230"
     CDS             complement(149..793)
                     /gene="maiA"
                     /locus_tag="VIR04_08230"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY9460772.1"
                     /translation="MKLYSYFRSSAAYRVRIALNLKSLSYETVPVHLLKEGGQQFSPE
                     YRALNPMALVPTLIDGDFVLTQSLAILEYLEETYPLPALLPFGAQDRARVRSIAQMIA
                     CDIHPLNNLRVLKYLKHELKADEEARNAWYRHWVSQGLTAVEKVLVASPQTGRYCHGD
                     APSLADACLIPQVANARRLDCNLSGVPVIVRIDAACAKLPAFEQAAPARQADAE"
     gene            complement(790..1764)
                     /gene="ispH"
                     /locus_tag="VIR04_08235"
                     /gene_synonym="lytB"
     CDS             complement(790..1764)
                     /gene="ispH"
                     /locus_tag="VIR04_08235"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY9460773.1"
                     /translation="MSVAQDRAQPDVEIVLAKPRGFCAGVDRAIDIVERALELHGAPI
                     YVRHEIVHNRYVVEDLKKKGAIFIDELDQAPKGAYVIFSAHGVAKKVWSQAEQMGLNV
                     FDATCPLVTKVHVEVARMRAAGREIIMIGHRGHPEVEGTLGQADEGMYLVENVQDVAN
                     LQVRDPSKLAFVTQTTLSVDDAASVAQALRTRFPGIMEPKKSDICYATQNRQDAVKIL
                     APQCDLVLVVGSPNSSNSNRLREVAERKGVTAYLIDDPAMIDPAWLRQSKRVGVTAGA
                     SAPEVLVQQVIGRLKELGAASVRTLPGAKENVSFPLPKELSRKLHETP"
     gene            complement(1764..2240)
                     /locus_tag="VIR04_08240"
     CDS             complement(1764..2240)
                     /locus_tag="VIR04_08240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY9460774.1"
                     /translation="MTSASDSVNAFVRPDSYLTLHYRIELASGPGAGSVFIDTFAGHP
                     ATLQLGSGQWAPGMEQALVGREEGTRFNIELTAAQAYGERNPDLLQRVTMTMLVENTP
                     PDTHFDPGDLVEFSAPNGVRYSGVLKELGDTWALFDFNHPLAGTDLHIEVSLLGVL"
     gene            2293..2970
                     /gene="radC"
                     /locus_tag="VIR04_08245"
     CDS             2293..2970
                     /gene="radC"
                     /locus_tag="VIR04_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY9460775.1"
                     /translation="MTFHTAPPNPERPRERLLAHGAHVLTSPELLAIILRTGTKGCSA
                     ISLGHKLITQFKGLRGLLAADAQTLLAIKGLGAAKTCELLAINELNRRALEEELKAGQ
                     ALDQPQRVKHYCTANLGHLQVEHCMALYLDSQYRLIASEELSRGTLTQASVYPREVIK
                     AGLRHHAAALILAHNHPSGVAEPSEADLALTRHLKHALALVDIRLLDHLIVTASGATS
                     LAERGQV"
     gene            3146..3412
                     /locus_tag="VIR04_08250"
     CDS             3146..3412
                     /locus_tag="VIR04_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEY9460776.1"
                     /translation="MKEGIHPEYRDVVFLDQQTGTKFISRSTLTSRETVDVDGKTYPL
                     FKCDVTSASHPFYTGAQTRTVETGRVEKFRARFARTAGTVKKAS"
     gene            3507..>4459
                     /locus_tag="VIR04_08255"
     CDS             3507..>4459
                     /locus_tag="VIR04_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9460777.1"
                     /translation="MAPDDSRSTPARLTAPATVKLPRLFLLVLGVVYIFAGLFFRDPW
                     KTDDVVGLATMLTALHGPGHALLLPQIGALAHAQDGPLPMWVGAFSIWLFSPIFEIFT
                     TKLDAAIVASRLPNLLWFGLLTSSVWYGAYLLGRRPEPQPLALPFGGEPTVRDYGRMI
                     ADAALLLIVATVGILWRMHETSEVPALIAFQALAFYAVARMLDRPVSGAITLGIALAA
                     AFSTRGWIGSLPIMLAIPFIFTPRGALWPSKHWLIVTAVVAISLVLLWWIPANRIGDY
                     WMRNWWLWNTRSFTWPHLETTLSTLRDLSWFLWPTWPFALLA"
BASE COUNT          938 a         1370 c         1181 g          970 t
ORIGIN      
        1 gcggcgggtg tcgaagcggc gcgcgcaacc accgccgagg ccttcgtcga cctattgcgc
       61 tcaggcctgg ccagaaaagg gccattcctt attgaggcaa tcatttaaaa cattcccggg
      121 cctgtgacgg cgcaggcaat aaggctggct attccgcgtc ggcctgccgg gccggcgccg
      181 cctgttcaaa cgccggcaat ttggcgcacg ccgcatcaat ccggacaata accggcacgc
      241 cgcttaagtt gcagtcaagg cggcgcgcat tggctacttg gggaatcagg caggcatcgg
      301 ccaatgaagg agcatcgcca tgacaatagc ggcctgtctg cgggctggcg accagcacct
      361 tctccaccgc tgtcagacct tgcgacaccc agtgacgata ccaggcgttt cttgcttctt
      421 catcggcctt taattcatgt ttcaagtact tcagcacccg caaattattt agcggatgaa
      481 tatcgcacgc gatcatttgc gcaatggagc gcacccgtgc ccgatcctgc gcaccaaacg
      541 gcaaaagagc cggaagcggg taggtctctt cgaggtactc gagtatggcc agtgactgcg
      601 tcagcacgaa atcgccatct atcagcgtgg gaaccaatgc catcggattt aacgcgcggt
      661 actcgggcga aaattgctgg ccgccttctt taagcagatg cacaggcact gtctcgtaag
      721 acagactttt aaggtttaat gcaatccgca cgcgataagc cgccgaactg cgaaaataac
      781 tgtatagctt catggtgttt cgtgcagctt gcgcgaaagc tccttgggca aaggaaacga
      841 cacgttttct ttggcccccg gcaaggtgcg taccgacgcc gcgcccagct ccttcagacg
      901 gccgataacc tgctgcacca gcacttccgg agccgatgcg cccgccgtta ccccgacccg
      961 tttgctttga cgcagccagg cgggatcaat catggcggga tcgtcgatca aatacgccgt
     1021 gacccctttg cgttccgcca cctcgcgcag gcgattcgaa ttcgaactat tggggcttcc
     1081 gaccaccagc accaaatcgc attgaggcgc caaaattttg acggcatcct gacgattttg
     1141 agtcgcgtag caaatatcgc ttttcttggg ctccatgatg cctgggaaac gggtgcgcaa
     1201 cgcctgcgcc accgaggcgg cgtcatccac cgacagcgtt gtctgcgtta cgaaggcaag
     1261 cttgctggga tcgcgcacct gcaggttcgc cacgtcctgc acattttcca ccagatacat
     1321 gccttcgtcg gcctggccca gagttccttc aacttcgggg tggccgcgat gaccgatcat
     1381 gatgatctcg cgccccgccg cacgcatccg ggctacctcg acatggacct tggtcaccag
     1441 gggacaggtg gcgtcaaaga cattcaaacc catctgttcg gcctggctcc atactttttt
     1501 cgccactccg tgggccgaaa aaataacgta tgcgcctttg ggcgcctggt cgagctcatc
     1561 gataaaaata gcgccctttt tcttcaggtc ttcaaccaca tagcggttgt gaacaatttc
     1621 gtggcgtaca taaatgggcg cgccatgcaa ttcaagcgca cgctcgacaa tgtcgatggc
     1681 gcgatcaacc ccggcacaaa agcccctggg tttcgccaga acaatctcga catcgggctg
     1741 cgcccgatcc tgcgctacag acattacaaa acccctagta aagatacttc aatatgcaaa
     1801 tcggtccctg ccagagggtg attgaaatcg aaaagcgccc acgtgtcgcc cagctcttta
     1861 agcacgcccg aatagcgtac accgtttggc gccgaaaact cgactaaatc gccaggatca
     1921 aaatgggtat cgggcggcgt attttcgacc agcatggtca tggttacccg ctgcaaaagg
     1981 tcggggttgc gttcaccgta tgcctgcgcc gccgtcaact cgatattgaa acgcgtgccc
     2041 tcttcgcgcc ccaccagagc ctgctccata cctggcgccc attgcccgct gccaagctgc
     2101 aacgtggcgg gatggcctgc gaacgtatcg ataaacacgg agcccgcacc gggtccggag
     2161 gcaagctcaa tacgatagtg caaggttaaa taagagtcag gccggacaaa agcattcact
     2221 gagtcagaag ccgaggtcaa aatctgtcac cgtagaggat cgttgctatt gacccctatt
     2281 ttagagcctc ttatgacatt tcacacggcc ccgccaaatc ccgagcgccc gcgcgaacga
     2341 ttgctcgccc atggcgcgca tgttctgact tctccggagc ttctggccat tattttgcgc
     2401 acgggaacca aaggctgttc cgccatatcc ctgggtcata aactcatcac ccagttcaag
     2461 ggtttgcgcg gcctgctggc ggccgatgct caaacgcttc tggcgattaa aggcctgggc
     2521 gccgcaaaaa catgcgaatt gctggccatc aatgaactaa accgccgcgc cctcgaagaa
     2581 gaactgaaag caggccaggc gctggatcag ccacagcggg tcaaacacta ctgcaccgcc
     2641 aacttggggc acctgcaagt cgagcactgc atggctttgt atctcgacag tcaataccgc
     2701 ctgatcgcca gcgaagaact gtcgcggggc acccttaccc aagcctccgt gtatccgcgc
     2761 gaggtgatca aagccggcct gcggcatcat gccgctgcgc tcattctcgc gcacaaccac
     2821 ccatcgggcg ttgccgaacc cagcgaagcc gacctggcgt taacacgcca tctgaaacat
     2881 gcactggcac tggtcgacat acgtttgctc gatcacctga tcgtcacggc atcgggtgcc
     2941 acatccctgg ccgaacgcgg ccaggtttaa acaagccctg gatcaatgcc atggcgtctc
     3001 accgaatctg tgcgcatttg tgcaaaccat ttttgccggc cggtgatagc catgctaaaa
     3061 tcgttggcta attactggat acgtggcctt aagtttatct ggctttggct ggcgacccgc
     3121 ccattttgaa aaacaggaat tcatcatgaa agaaggcatc caccctgaat accgcgacgt
     3181 ggtgtttctg gaccagcaaa ccggtacgaa atttatttcg cgctcgaccc tgaccagccg
     3241 cgagacggtc gacgtcgatg gcaagacgta ccctttgttc aaatgcgacg taacttctgc
     3301 atcgcatccg ttttatacgg gagctcaaac gcgcaccgtc gagacgggcc gggttgaaaa
     3361 attccgcgct cgttttgcac gcacggcagg cactgtcaaa aaagcttcgt aagccttcac
     3421 cgcttgcgac agtttataaa ggcagcctta cggctgcctt tataatttaa gacggcccat
     3481 tcctattttc ttatagcgcg cacaccgtgg ctcccgatga ttcccgctct acacccgccc
     3541 gcctgaccgc gccggcaacc gtaaaactgc ccagactttt tttattagtc ctgggcgtgg
     3601 tttatatctt cgcgggcctg ttcttccggg atccatggaa aaccgacgac gtggttggcc
     3661 tggccaccat gctcacggcc ctgcacggtc ccggtcatgc gctgctgctt ccgcaaatcg
     3721 gggccttggc acacgcacaa gacggcccgc tgcccatgtg ggtgggcgcc ttcagcatct
     3781 ggctcttcag cccgattttc gaaattttca cgacgaaact ggatgcggcg attgttgcct
     3841 cgcgcctgcc taacctgctc tggttcggcc tgctcaccag ctcagtctgg tatggcgcct
     3901 atttgctggg acgacgtccg gaaccgcaac cgctggcgtt gccgttcggt ggcgaaccga
     3961 ccgttcgcga ttacggccgc atgatcgccg acgcggcgtt gctgcttatc gtggccactg
     4021 tcggcatctt gtggcgcatg cacgaaacgt ctgaagtgcc ggcccttatt gcttttcagg
     4081 cgcttgcgtt ttatgcagtg gcgcgcatgc tggaccgtcc cgtatcgggc gccatcaccc
     4141 tgggaatagc actggcagcg gcattttcaa cgcgcggctg gatcggctcc ctgccaataa
     4201 tgctcgccat tcccttcata ttcacaccgc gtggcgcgtt atggccatcg aaacactggc
     4261 tcatcgttac cgcggtcgtt gccatatcgc tggttttgtt atggtggatt cccgccaacc
     4321 gtatcggcga ctattggatg cggaactggt ggttgtggaa cacccgatcg ttcacgtggc
     4381 ctcatctgga gaccaccttg agcaccttgc gcgacctgtc gtggttctta tggcccacat
     4441 ggcctttcgc tttgctggc
//
LOCUS       DATCXU010000368         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_823769,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000368 DATCXU010000000
VERSION     DATCXU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823769"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VIR04_08260"
     CDS             complement(<1..1603)
                     /locus_tag="VIR04_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY9460778.1"
                     /translation="MKRMLFNATHQEELRVAIVDGQKLIDLDIETAGREQRKGNIYKG
                     VITRIEPGLEACFVSYGEERHGFLPFKEVARSYFKEGVDVRTARIQDALAEGQELIIQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLP
                     AGMSIIARTAGIGRGVEELQWDLSYLMQLWNAIDSAARDNKAPILIYLESSLVIRAIR
                     DYFSPEIGEILIDTDDIAEQATAFMSVVMPDNVQRVKIYRDDVPLFSRFQIEHQIETA
                     YSRTVELPSGGSIVIDHTEALVAVDVNSARSTRGADIEETATRTNLEAADEVARQLRL
                     RDLGGLIVIDFIDMEEGKNQRSVETRLRDALHFDRARVQMGKISRFGLMELSRQRLRP
                     ALNEGSHITCPRCNGTGVIRDAESSALQILRLLQEEAMKENTAALYAQVPVDVATFLL
                     NEKRVDISKLESRLKIKLVLIPNKNLETPHHHIERIRHDDPRLEESKPSFELVENPDL
                     EPSWTPDKEHEAKARPEALVKGITPT"
     gene            2107..>2380
                     /locus_tag="VIR04_08265"
     CDS             2107..>2380
                     /locus_tag="VIR04_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY9460779.1"
                     /translation="MRKPESYKDSIPAVRFLQIDAQNEGQRVDNFLLRLCKGVPKSHL
                     YKAIRNGEVRVNKGRVSADYRLVPGDTLRVPPLRLPRADAPAVVPPA"
BASE COUNT          482 a          644 c          689 g          565 t
ORIGIN      
        1 gagttggtgt gattcctttg accagggcct cgggccgcgc tttcgcttcg tgttctttat
       61 cgggcgtcca ggaaggttcg agatccggat tctcgaccag ttcgaagctg ggcttggact
      121 cttccaggcg cgggtcgtcg tggcgtatgc gttcgatgtg atggtgcggg gtctcgagat
      181 ttttgtttgg aatcaggacc agcttgatct tcagtcgcga ttccagtttt gaaatgtcga
      241 cgcgtttttc attcagcagg aaagtggcaa catcgacggg cacttgcgcg tacaaggcgg
      301 cggtgttttc tttcatggct tcttcttgca gcaagcgcaa aatctgcagc gcgcttgatt
      361 cggcatcgcg aatgacgccg gttccgttgc aacgcgggca agtgatgtgc gagccttcgt
      421 tcagggccgg gcgcaggcgc tggcgtgata attccatcaa gccaaaacga gatattttcc
      481 ccatctggac ccgggcgcga tcgaaatgca gggcgtcgcg caggcgcgtc tcgacagagc
      541 gttggttttt tccttcctcc atgtcgatga agtcgatcac aatcaggccg ccaaggtcgc
      601 gcaaacgcaa ctggcgggcg acttcgtcgg cggcttccag attggtgcgt gtggcggtct
      661 cttcgatgtc ggcgccacgg gtggagcgcg ccgagttgac atcgacggcg acgagcgctt
      721 cggtgtgatc aataacaatc gatccgcccg agggaagctc caccgtacgc gaatacgccg
      781 tttcaatttg atgctcgatt tggaagcgcg agaataatgg aacgtcgtcg cgataaattt
      841 tgacccgttg gacgttgtcg ggcatgacga cgctcatgaa cgcagtggcc tgctcggcta
      901 tgtcgtcggt atcgatcagg atctcgccga tttcgggcga gaaatagtcg cgaatggcgc
      961 gaattaccag acttgattca agataaatga gaatcggcgc cttgttatcg cgcgcagcgc
     1021 tgtctatcgc attccagagc tgcatcaaat aggaaagatc ccactgcaat tcttcgacgc
     1081 cccgcccgat gcccgccgta cgagcaataa tgctcatgcc ggccgggaga tcaagtttgt
     1141 ccatcgtttc acgcaattcc tgacggtctt cgccctcgac gcggcgtgat acgccgccgc
     1201 cacgcgggtt attgggcatc aggaccagat aacggcccgc cagcgatata aaggtggtta
     1261 gcgccgcgcc tttgttgccg cgctcttcct tttcgacctg gatgatcagt tcctgacctt
     1321 cagccagcgc atcctgaatg cgcgcagtgc gcacatcgac accctctttg aagtaacttc
     1381 ttgcgacttc cttgaatggt aaaaagccgt gccgttcttc gccatagctg acgaagcagg
     1441 cttccaggcc gggctcaatg cgggtaataa cgcctttgta aatgtttccc ttgcgttgtt
     1501 cgcgaccggc cgtttcgata tcgagatcga tgagcttttg cccgtcgaca atggcaacgc
     1561 gtaattcttc ttgatgcgtt gcattaaaca gcattcgttt catgattatt tttctccgta
     1621 gtcatggcgc gccgtttatc ggcgcacgac cgcgggtcat cgggtgtgtg gcgctggatc
     1681 gagcaataag gaggctaagc cgacaatgtc aggctatgcc tatcctgggg caggcaggca
     1741 cggcgtgtct gtggacacgt agttcaatca gcaggagggt cagattcacg atagtggttg
     1801 acaaaagatt gctgggaacg gcaggcgcgt aaccaaattc gcggctggtg cttaaacgga
     1861 taacctgtat tgcaagaact atagcgccgc acgcggctgg caatcgatcc gggggaaaca
     1921 gcgggcacac gtgccacacc actgtcgtgc ggaccaggat taccccggcg acctaatgac
     1981 ccagaagctg aaactcgggc agtacaatgt gattacaggg cctaacggag acgcatccat
     2041 tgcatctcta cgttcggcgg tttttcagct tctataggct gacgcgatta taggctgctt
     2101 ttctccatgc gcaaacctga aagttacaaa gattccattc cagccgtgcg cttcttgcaa
     2161 attgatgccc aaaacgaagg gcagcgcgtc gataatttcc tgctgcgttt atgcaaaggg
     2221 gtgcctaaaa gccaccttta caaagccatt cgcaacggcg aggtgcgtgt caacaagggc
     2281 cgtgtttcgg ccgattaccg ccttgttccg ggggatacgt tgcgcgtgcc gcctttgcgc
     2341 cttccccgtg ccgatgcgcc ggctgtcgtc ccgccggcgg
//
LOCUS       DATCXU010000369         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_686373,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000369 DATCXU010000000
VERSION     DATCXU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686373"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..512
                     /gene="rplS"
                     /locus_tag="VIR04_08270"
     CDS             123..512
                     /gene="rplS"
                     /locus_tag="VIR04_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY9460780.1"
                     /translation="MDLIAILEQEEINRLTGGVPKPQFSAGDTVVVNVNVVEGARKRI
                     QAYEGIVIAKRNRGLNSSFIVRKISSGEAVERTFQLYSTQIAGIEVKRRGDVRRAKLY
                     YLRNLSGKAARIKEKLVRNSAKKAAAK"
     gene            594..1355
                     /locus_tag="VIR04_08275"
     CDS             594..1355
                     /locus_tag="VIR04_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY9460781.1"
                     /translation="MSTMSQSTLPPIRDRRVVVPGFDPQTQPIVATKALVPLSPATLE
                     LDFICAAFNSPVDWHVEPVFSASFSSNFLDCNDIVRAAVFVPLVQRPSGLSVIFTRRS
                     PQLYDHAGQISFPGGRIEPSDRDAIAAALRETQEEIGVAPQFVQLIGTQPGFLTSTRF
                     TMQPVIGLVRPGFTITPAPDEVAEVFEVPLSVLMDPARHCLHQAQLAGGGHRFYFSIT
                     WHSYFIWGATAALIRNFYHFLAAAQTGLDPNRRLS"
     gene            complement(1397..2302)
                     /gene="rsgA"
                     /locus_tag="VIR04_08280"
     CDS             complement(1397..2302)
                     /gene="rsgA"
                     /locus_tag="VIR04_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY9460782.1"
                     /translation="MAENATARGRIIAAHGRHYTVELENGEQRKCFPKGKKAGAAVGD
                     FVEVSLQGATEGVIDRIFERRNLLYRSDDMRTKQFAANVDQLLIVVAVEPAVSGDLTG
                     RALVAARSADIEPLIILNKIDIKNKLPAARSNLASFAALGVRVLEFSALNVDQVRALL
                     LPLLKGRTSLLLGQSGMGKSTILNALAPQALAYTREHSLALDAGKHTTTSTRLYHLPE
                     NGGDIIDSPGFQAFGLNHLSPADIEHGFPEFKDAIPFCRFYNCTHRHEPGCGVLAALK
                     RGEISPERHGLYRRILAENEAKARY"
     gene            complement(2295..3539)
                     /locus_tag="VIR04_08285"
     CDS             complement(2295..3539)
                     /locus_tag="VIR04_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY9460783.1"
                     /translation="MFTLFFIAFLLTEILLRLWLATRQIRHVKENREHIPVEFSERVS
                     LHSHQRAADYTLAKMRLNMLERVADAAILVGFTLLGGLQYLDTHLGLAIENEMGRQLA
                     LIGSVVALLGLLNIPFSAWRKFGLEARFGFNRVTPGLFITDALKGLLISVLLGAPLAA
                     SLLWVMAHAGAYWGWWAWIIWVGFSFLILWLFPTVIAPLFNKFTPLDNPEISERVYGL
                     ARRCGFALRGLFVMDGSKRSAHGNAYFTGFGKARRIVFFDTLLARLNTDEIEAVLAHE
                     LGHFKHRHVIKRMIASFGLALVFLQILSWLCGQTWFYTGLGVVPQFGRPNDALALILF
                     FLAMPVFTFWATPLASWLSRRDEFQADRYAVTQCSAPPLISALVKLYDDNAATLTPDP
                     IHSAVYDSHPPAVVRIQHLKHG"
     gene            3587..4132
                     /gene="orn"
                     /locus_tag="VIR04_08290"
     CDS             3587..4132
                     /gene="orn"
                     /locus_tag="VIR04_08290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY9460784.1"
                     /translation="MAVNDQRLVWLDMEMTGLDPEKERIIEVAMVVTEPDLTFVAQAP
                     VLVIHQSDALLDAMDKWNKSTHGKSGLIEKVRASTLTEQQAEEQLLAFLSQYVPSGKS
                     PMCGNTIGQDRRFMNKYMPRLEAYFHYRNLDVSTLKELSLRWKPEVYKSFVKKSCHEA
                     LADINESIEELKHYREHFIKL"
     gene            4178..4606
                     /gene="acpS"
                     /locus_tag="VIR04_08295"
     CDS             4178..4606
                     /gene="acpS"
                     /locus_tag="VIR04_08295"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY9460785.1"
                     /translation="MLHGSIAGVGVDLLRIERIERVYVRHGSRFIQRILGEQEIEKFQ
                     RRYQRDPRRGIRFLASRFAAKEAFSKAIGLGMHSPMAWSRMQTVNEPGGKPCVRLDEP
                     LASWYARRFGVAHVSITDESDTVVAFVVVETLNKEPDHGR"
     gene            4596..5654
                     /gene="nagZ"
                     /locus_tag="VIR04_08300"
     CDS             4596..5654
                     /gene="nagZ"
                     /locus_tag="VIR04_08300"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY9460786.1"
                     /translation="MAADPLRTLLAPGPVMVDVAGLELTTHEKQRLRHPLVGGVILFA
                     RNFSNRAQLVRLCQAIHAERNEPLLIAVDHEGGRVQRFRTDGFTTIPAMGVLGEIWMR
                     DPMLSMRLATEAGYVTGAELRACGIDLSFTPVLDLDYGISEVIGNRAFHRDPRVVTML
                     ARALIQGLMLAGMGACGKHFPGHGCVAADSHRQIPVDGREIQEILQEDAAPYGWLGDM
                     VLPAVMPAHVIYSKVDPLPAGFSRYWIQKVLREDLAFDGLVFSDDLTMEGATVAGDIL
                     GRAQAALHAGCDMVLVCNRPDLADDLLNRLVFDQPDASVNRIRRLMPKCPSVNWNSLQ
                     ANERYHYARRLQSQIVSG"
     gene            5617..>6532
                     /gene="uvrC"
                     /locus_tag="VIR04_08305"
     CDS             5617..>6532
                     /gene="uvrC"
                     /locus_tag="VIR04_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY9460787.1"
                     /translation="MPDDFNLKSFLDDLPHLPGVYRHIDAAGEVLYVGKARDLKKRVT
                     SYFRKNLDSPRIAHMVARVVRLEITVTRSEAEALILENNLIKTLKPRYNILFRDDKSY
                     PYLMISDHDIPRIAYYRGGTSGKGRFFGPYPNSWAVRETIQILQRVFRLRTCEDSVFA
                     NRSRPCLLYQIGRCSAPCVGLISREDYRADVDRAIQFLEGHAKDVMRDIEKRMRDASA
                     ARRFEQAAVLRDQMGALARVLQQQSMEKIGSADVDVIAIASAGGRVCVNLAMVRGGRH
                     LGDRPFFPVQTQGDDAGDVLAAFVTQHYL"
BASE COUNT         1434 a         1724 c         1836 g         1538 t
ORIGIN      
        1 ggtcgctggc ttttaaatta cgccgtttag ctgtagaata ctgggttttg ttaactcatc
       61 ctctggcggc gggcttaaac aataataccg ataccgaatc atgatgaaac ctggagctta
      121 ctgtggatct aattgccatt cttgaacaag aagaaatcaa tcgtttaacc gggggggttc
      181 ccaaaccgca attttccgcc ggcgataccg tcgtggttaa tgtgaacgtt gtcgaaggcg
      241 cccgcaagcg tattcaggcc tatgaaggca tcgttattgc aaagcgcaat cgtggcttga
      301 actcatcgtt tattgttcgc aaaatttctt cgggcgaagc agttgagcgc acgttccagc
      361 tttactccac ccaaattgca ggcatcgaag tcaagcgccg tggcgatgtg cgtcgggcca
      421 agctttatta tttgcgaaat ctttcgggca aggcggctcg catcaaagaa aaactggttc
      481 gtaattcggc aaaaaaggcc gctgcgaagt aattgctggc ccggtgtctg tcgcgcagtg
      541 tgtcaggcac taactggtaa aatgataaag gcgctcagaa ggcgccttta cttatgagca
      601 ccatgtcgca aagcacgtta cctcccattc gtgatcgacg cgtcgttgtt cctggcttcg
      661 atccacagac tcaacctatt gtggctacga aagctttggt cccgctttcg cccgcgaccc
      721 tggaattgga tttcatttgc gccgcgttta attccccggt tgactggcat gtcgagcctg
      781 ttttttccgc ctcattcagt tctaattttt tggattgcaa cgatattgtc cgggctgctg
      841 tatttgtccc actagtgcaa cggccgtcgg gcctaagtgt tatttttacg cggcgatctc
      901 ctcagttgta cgaccatgcg gggcaaatca gctttccggg cggacggatc gagccatctg
      961 atcgcgatgc cattgccgcg gcgttgcgcg aaacccagga agaaataggc gtagcgcctc
     1021 aattcgtgca actcatcgga acccagcccg gttttttgac gtcgacgcgt tttaccatgc
     1081 agcccgttat tggtctggtg cggcccgggt tcacgattac gcctgcgccg gacgaagtgg
     1141 ccgaagtttt tgaagtgccg ctctcggtat tgatggaccc tgcccggcat tgtttacatc
     1201 aggctcagtt ggccggcggc gggcatcggt tctacttttc catcacgtgg cactcttatt
     1261 tcatatgggg cgccaccgcg gcattaattc gcaattttta tcactttctg gcggcggccc
     1321 aaaccggact cgatccgaat cgcaggctgt cctagcagcg gttcgggctt ggctccgggg
     1381 ctccacccgc agctttttaa tagcgcgcct tggcttcatt ttcggccaga atgcgccgat
     1441 agagcccatg ccgttcgggg ctgatttcac cccgtttcaa agcggccaga acgccgcagc
     1501 caggttcatg gcggtgcgtg caattgtaga atcggcaaaa aggtatggcg tccttgaatt
     1561 cgggaaatcc atgttcaatg tcggcggggc taagatgatt aagaccgaaa gcctgaaacc
     1621 ccggtgaatc aatgatgtcg ccgccgtttt ccggaaggtg gtataagcgt gtgctggtcg
     1681 ttgtgtgctt gcccgcatcc agcgctagcg agtgttctcg cgtgtaagcc agcgcctgag
     1741 gcgccagcgc gttaagaatc gttgattttc ccattccgct ttgacctagc agcaagctgg
     1801 tgcggccttt gagcaaaggc agtaataatg cccggacctg atcaacgttc agcgcgctga
     1861 attcaagaac gcgtacgccc aacgcggcaa agcttgccag gttcgatctg gcggctggca
     1921 gtttgttttt gatgtcgatt ttgttcagaa tgatcaaagg ctcaatgtcg gcgctgcgtg
     1981 ctgccaccag tgcacgcccc gtcaaatccc ccgagaccgc cggttcgacg gccacaacaa
     2041 tcaacaattg atctacgttc gcggcgaact gcttggttcg catatcatca gagcgataca
     2101 gcaggttgcg gcgctcgaat atccggtcaa tgacgccttc ggtggcgcct tgcagactga
     2161 cctcgacaaa atcacccaca gcagcgccgg cctttttccc ttttggaaag catttccgct
     2221 gttcgccgtt ttcaagctcc acggtgtaat gtcgcccgtg tgcggcaata atgcgtcccc
     2281 gagccgtggc gttttcagcc atgtttcaga tgctggatgc gcactaccgc cggtggatgg
     2341 ctatcgtaga ctgcggagtg tatggggtcg ggcgttaacg tggctgcgtt atcgtcatac
     2401 agcttgacca gcgccgatat caatggcgga gccgagcatt gtgtgacggc gtaacggtct
     2461 gcctgaaatt catcgcggcg tgaaagccag cttgccaaag gtgttgccca gaatgtaaag
     2521 accggcatcg ccaggaaaaa taaaatcaac gccagggcgt cgttcggcct cccaaattgc
     2581 gggacgacgc ccagacctgt atagaaccat gtttgtccgc agagccagct tagtatctga
     2641 agaaacacca gcgcgaggcc gaagcttgca atcattcgtt taatgacatg cctgtgcttg
     2701 aagtggccca gctcgtgggc cagcaccgcc tcgatttcgt ccgtgttcaa acgcgcgagc
     2761 aaggtatcga aaaatactat ccttcgggcc ttgccaaaac ctgtgaagta ggcgttgccg
     2821 tgggccgaac gtttcgagcc gtccataaca aaaaggccgc gcagtgcaaa gccgcagcgc
     2881 cgggccagcc catagacccg ttcggagatt tcaggattat ccaacggcgt gaatttgttg
     2941 aataacgggg cgataacggt tggaaagagc catagaatca agaaactgaa gccgacccaa
     3001 atgatccatg cccaccagcc ccaataagcg cccgcatgag ccatgaccca caatagactg
     3061 gcagccagcg gcgcgcccaa cagcaccgag attagcaagc ctttcagcgc atcggtaatg
     3121 aacaacccgg gagtgacgcg gttaaaaccg aatctggctt cgagcccgaa tttgcgccac
     3181 gccgaaaagg gtatgttcaa tagccccagc aacgccacta cggaaccgat cagcgccagt
     3241 tgacgcccca tctcgttttc gatggcaagg cccaagtgcg tgtccagata ctgcaaaccg
     3301 cccaataaag tgaaacccac cagtatggcg gcgtcggcga cccgctcgag catattgagc
     3361 cgcatttttg ccaaggtgta gtcagccgcc cgttgatggc tgtgcaggct gacccgctct
     3421 gaaaattcaa ctggaatgtg ctcacgattc tccttgacgt ggcgtatttg gcgcgttgcc
     3481 agccataaac gcagcaaaat ctccgtcagc aaaaaggcaa taaaaaacaa tgtgaacata
     3541 aggggttttg ctttatgcga aaatcgatgt tttaaggaag aattatatgg cggttaatga
     3601 tcagcgattg gtgtggctcg atatggaaat gacgggtctc gatcccgaaa aagaacgcat
     3661 tatcgaggtc gcgatggtgg ttaccgagcc agacctgaca tttgtcgctc aggcccccgt
     3721 actggttata caccagagcg atgcgctgct cgatgccatg gacaaatgga ataagtccac
     3781 gcacggcaaa agcggcttga tagaaaaggt cagggcctcg acccttaccg aacaacaggc
     3841 cgaagagcaa ctgctggcgt ttttgagcca atatgtgcca tcgggaaaat ccccgatgtg
     3901 cggcaatacg attggtcagg accgccggtt catgaacaaa tacatgcccc gcttggaagc
     3961 ttacttccat taccgcaatc tggatgtcag cacactgaaa gaactttctc tgcgctggaa
     4021 gcccgaagta tacaagagct tcgtcaagaa aagttgtcac gaggcgctgg ccgacatcaa
     4081 cgagtccatc gaagaactaa aacattatcg cgaacatttc atcaagttgt gatcgtgcaa
     4141 tgatctgcga cagtattcaa tttacaggaa gttgaacatg ctccacggca gcatagcggg
     4201 cgtcggtgtc gatttattgc gcatcgagcg tattgaacgc gtttatgttc gtcatgggtc
     4261 caggtttatc cagcgtattt tgggcgagca ggaaattgaa aaatttcagc gccgctatca
     4321 gcgcgatccg cgacgcggca tacggtttct tgcctcccgg ttcgccgcca aagaggcgtt
     4381 ttcgaaggca atcggcttgg gaatgcatag tcccatggcg tggtcccgca tgcaaactgt
     4441 taatgaacca ggcggcaagc cctgtgtccg tctcgacgag ccgctggcga gctggtatgc
     4501 acgccgattc ggtgtggcgc atgtttcgat tacggacgaa agcgacactg ttgtagcgtt
     4561 tgttgtcgtg gaaacactta ataaggaacc cgaccatggc cgctgacccg ttgcgcacac
     4621 tcctggcccc gggccccgtc atggtcgatg tggccggtct cgaactgacc acgcatgaaa
     4681 agcagcgcct gcgccatcct ttggtgggcg gtgtcatctt gttcgcccgc aatttcagca
     4741 atcgtgcgca gcttgtccgc ctgtgccagg cgatccatgc cgaacgcaat gaacctttgc
     4801 tgattgcggt ggatcacgaa ggtggccggg ttcagcgttt tcgcaccgac gggtttacga
     4861 caataccggc catgggtgta ttgggcgaaa tttggatgcg cgatcctatg ctctctatgc
     4921 gcctggcgac cgaagcaggt tatgtaacag gcgctgaact gcgagcctgc gggatcgatt
     4981 taagctttac gcctgtgctc gatctcgatt acggaataag cgaagtgatt gggaatcgcg
     5041 cttttcatcg cgatccgcgg gtggtcacca tgttggcccg cgccttgata caaggtttga
     5101 tgctggccgg catgggcgcg tgcggcaagc attttccggg gcatggctgc gtcgccgccg
     5161 actcccatcg ccagattccg gtcgacgggc gcgaaattca ggaaattctg caagaagacg
     5221 ctgcccccta cggctggctg ggcgatatgg tgttgcccgc agtcatgccc gcgcacgtta
     5281 tttattctaa agttgacccg ttgcccgccg ggttttccag gtactggatc cagaaggttt
     5341 tgcgtgagga tcttgctttt gacgggcttg tgttttccga tgatctgacg atggaagggg
     5401 cgaccgtcgc gggcgatatt ctggggcgcg cacaagcggc gttacatgcc ggatgcgaca
     5461 tggttcttgt ttgcaatcgt ccggatttgg cggatgattt attgaacagg ctggttttcg
     5521 atcagcccga tgcgtcggtc aaccggattc gccggctgat gccgaagtgc ccttcggtta
     5581 attggaattc tttacaagct aatgagcgtt accactatgc cagacgactt caatctcaaa
     5641 tcgtttctgg atgatttgcc gcacctgccg ggcgtatatc ggcacattga tgcggcgggc
     5701 gaggttttat acgttggcaa ggcgcgtgat ttaaaaaagc gcgtcacctc ttattttcgg
     5761 aaaaatctgg atagcccgcg cattgcgcac atggtggcgc gtgtggtgcg tctggaaatt
     5821 acggtaacgc gatccgaagc ggaagcgctg atactggaaa acaatctcat aaaaacgctg
     5881 aagccgcgct acaacatttt gtttcgcgac gacaagtcct acccgtactt gatgatcagc
     5941 gatcacgata ttccccgaat cgcctattac cgtggcggca cgagcgggaa aggccgtttt
     6001 tttggcccct atccgaattc gtgggcggtg cgcgagacaa ttcagattct gcaaagggtt
     6061 tttcgtttgc gcacatgtga agacagcgtg tttgcgaatc gttcccggcc ctgtttgctt
     6121 tatcagatag ggcgctgctc ggcgccctgc gtcgggctga ttagccgcga ggactatcgt
     6181 gcggatgtgg atcgcgccat acagtttctg gagggtcatg ccaaagacgt catgcgcgac
     6241 atcgaaaagc gcatgcgcga cgcttccgcc gcgcgccgct ttgaacaggc ggcagtgttg
     6301 cgcgatcaaa tgggtgcgct ggcgcgcgtt ttgcagcagc aatcaatgga aaaaattggc
     6361 agcgccgatg ttgatgtgat tgcgattgcc tcggcgggcg gacgggtgtg cgttaatctg
     6421 gccatggttc gcggcggccg gcatctggga gacaggccgt ttttccctgt tcaaacgcaa
     6481 ggcgatgatg cgggcgacgt gctggcggcg tttgttacgc agcattatct gg
//
LOCUS       DATCXU010000370         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_709429,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000370 DATCXU010000000
VERSION     DATCXU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709429"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VIR04_08310"
     CDS             <1..201
                     /locus_tag="VIR04_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460788.1"
                     /translation="SVVNEVLDAVSQLREHTSILIVEQRVDLALRMVDRAYIMVNGHI
                     AYEGAALELKENKELQVKLLGV"
     gene            223..1005
                     /locus_tag="VIR04_08315"
     CDS             223..1005
                     /locus_tag="VIR04_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9460789.1"
                     /translation="MFDTISMKGVKALITAGGSGLGLEMARVFTAAGSEVFICDINKE
                     ALAAATAELPKLHAMIADVSDEASVAALFEQVRAQFGGLDVLVNNAGIAGPTGFVETL
                     AKADWDKTLAVNITGQFLCARLAIPLLKQSNAGVIINMSSAAGHLGFAGRAAYAASKW
                     AVVGFTKTLAIELGRFGVRVNAILPGAVEGPRIRAVIASKAQAMGIPNQELTQSYEQQ
                     ASLGRMVSARDIANMALFAASPAAASVNGQALAVDGHTQALV"
     gene            1024..1956
                     /locus_tag="VIR04_08320"
     CDS             1024..1956
                     /locus_tag="VIR04_08320"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY9460790.1"
                     /translation="MNVAIIGTGLIGQAWAIVFARGGCAVRLWDGDPVALKRALPLIS
                     AQVSELQKQRLLTGAAEIMTRIQSFDSLDKALDGAEYVQENLPEVLAIKKEIFSKLDK
                     LSSPDTVLASSTSSIPASAFTEDLPGRSRCLVAHPVNPPYLIPAVELCGAPWTDKKSI
                     EAARSIMTAVRQKPVVVNKELEGFVLNRLQGALLREAFRLVQGGYVSVEDLDVTVKDG
                     LGLRWSFMGPFETIDLNAPGGLEDYCRRYGGMYQSIAKEQTSTNAWDADLIAKVEKQR
                     RDQLPKSELLNRRLWRDERLMALLRQRKESGSSE"
     gene            1984..>2121
                     /locus_tag="VIR04_08325"
     CDS             1984..>2121
                     /locus_tag="VIR04_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY9460791.1"
                     /translation="MASKRKVIITCAVTGAIHTPSMSKYLPVTADEIAEAGIAAAKAG
                     AA"
BASE COUNT          496 a          570 c          646 g          409 t
ORIGIN      
        1 tcggtggtca atgaagtcct agatgccgtg tcgcagttgc gcgaacacac cagcatcctg
       61 atcgtggagc aacgggtgga tctggcttta cgcatggtcg accgcgccta cattatggtc
      121 aatggtcata tcgcctacga aggcgcggcg ctggagctca aggaaaacaa agaacttcaa
      181 gtgaaactgc tgggtgtatg aggggcttta acagaggtgg aaatgtttga cactataagc
      241 atgaaaggcg tgaaagcgct cattacagcc ggtggaagcg gtcttggcct ggagatggca
      301 agggtattta ccgccgcggg atccgaggta ttcatttgcg acatcaacaa agaggcgctg
      361 gcagcagcca cggccgagtt gccgaaactg catgcgatga ttgccgacgt ttctgacgaa
      421 gccagtgtcg ccgcgctgtt tgaacaggta cgtgcgcaat ttggagggct cgacgtcctt
      481 gtcaataacg cgggcatcgc aggccccaca ggcttcgtcg aaactctggc aaaggcggat
      541 tgggacaaaa ctctggccgt taacattacg gggcagtttc tttgtgctcg cctggccatc
      601 ccgctgctga agcagtcgaa cgcgggcgtt atcatcaata tgtcatcggc ggcaggtcat
      661 cttggttttg cgggacgcgc agcgtacgcg gcatcgaaat gggccgtggt cgggttcacg
      721 aaaacactgg ccattgaact gggccggttc ggcgtgcgcg tcaacgccat tttgccgggg
      781 gccgtcgaag ggccgcgcat acgcgccgtt atcgcttcca aagctcaggc catgggcatt
      841 ccgaaccaag agctgacgca atcatacgag caacaggcgt cgctgggccg catggtgtcc
      901 gcgcgcgata tcgccaacat ggccttgttc gcggccagcc cggcagccgc aagcgtgaat
      961 ggtcaggcac tggccgtcga tggacatacc caggcgctgg tttaacaaaa cagtggaaaa
     1021 actatgaatg tcgcaataat aggtacaggc ctgataggac aggcatgggc aattgtattt
     1081 gcgcgaggcg gttgcgccgt caggctgtgg gatggcgatc ctgtggcgct aaaacgcgcg
     1141 cttccgttaa tttccgcgca ggtgagcgaa ctgcagaaac agcgcttgct gaccggcgcc
     1201 gccgaaatca tgacgcgcat ccagtccttt gacagcctgg acaaagcgct tgatggcgcc
     1261 gagtacgttc aggaaaacct gccggaggtg ctcgccatca aaaaggaaat cttcagcaag
     1321 ctcgacaagc tcagcagccc ggacactgta ttggcgagct caacctccag catcccggcg
     1381 tcggccttta ccgaagactt gccaggccgc agtcgctgcc ttgtggcgca cccggtgaat
     1441 ccgccctatc ttattccagc cgtcgagctg tgcggtgcgc catggacgga caaaaaatcc
     1501 attgaagccg cccggtccat catgacggcg gtcaggcaaa agccagttgt tgtaaacaaa
     1561 gagctggaag gtttcgtgct caatcgtctg caaggcgcac tgttgcgtga agcattccga
     1621 ctggttcaag gcgggtatgt cagcgtcgag gatctggacg ttaccgtgaa agacggcctg
     1681 ggattgcgct ggtcctttat ggggccgttc gaaaccatcg acctgaacgc gccgggcggg
     1741 ctggaagact actgccgccg ctacggcggc atgtatcaat cgatcgccaa agaacaaacc
     1801 agcacaaacg cctgggacgc cgatctgata gcaaaggtcg aaaagcagcg gcgcgaccag
     1861 cttccaaaat cagaactgct gaaccgccgg ctatggcgcg atgaacggct gatggccttg
     1921 ttgcggcaaa ggaaagaatc gggttcttcg gaataattga tccatcgtct ggaaggaaaa
     1981 acaatggcaa gcaaaagaaa ggtaatcatc acgtgcgctg tgacaggcgc cattcacaca
     2041 cccagcatgt ccaagtattt gccggtgacc gccgatgaaa tcgccgaagc tggaatcgcg
     2101 gcggcgaagg cgggcgccgc c
//
LOCUS       DATCXU010000371         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_189668,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000371 DATCXU010000000
VERSION     DATCXU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189668"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /gene="uvrA"
                     /locus_tag="VIR04_08330"
     CDS             <1..1534
                     /gene="uvrA"
                     /locus_tag="VIR04_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY9460792.1"
                     /translation="AMPLSACLAWFQALTLAGAKKEIAERIVREIEARLQFLNNVGLN
                     YLSLDRSADTISGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNNRLIETLQHL
                     RDLGNSVIVVEHDEDMIRSADFIVDMGRGAGEHGGEIVAQGSAQDIILNPRSLTGQYL
                     SGTRRIDIPKRRPVSPDGPWLRLNGCTGNNLKSVNLAIPDGRLVCITGVSGSGKSTLI
                     NDTLATAVAQQLQRAQTEPAPYQSLEGLSLFDKIINVDQSPIGRTPRSNPATYTGMFT
                     PIRELFSEVPESRTRGYGPGRFSFNVKGGRCEACQGDGVVKVEMHFLPDMYVPCDLCE
                     GKRYNRETLEIRYRGRNISEVLNLTVEQALTYFEAVPSIARKLNTLMDVGLSYIRLGQ
                     SATTLSGGEAQRVKLSLELSKRGTGRTLYVLDEPTTGLHFQDIAMLLDVLRQLVDSGN
                     TVVIIEHNLDVIKTADWIVDLGPEGGNGGGYIVAQGTPEQIAANPDSYTGKYLQPLLM
                     HH"
     gene            complement(1583..2707)
                     /locus_tag="VIR04_08335"
     CDS             complement(1583..2707)
                     /locus_tag="VIR04_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY9460793.1"
                     /translation="MTELAAYACYPDQSRGRAHSEEPPVNRSQFQRDRDRIIHCNAFR
                     LLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARTLARNLGLHEDLTEAISLAHDLGHTP
                     FGHAGQDELNACLRELAPDAGGFEHNLQSLRVVDELEERYAEFNGLNLCFETREGILK
                     HCSVKNARVLGDVGERFLNKTRPSLEAQIANLADEIAYNNHDVDDGLRSGLITVDQLQ
                     ELEIFASHCARVRRKYPGLAQRRVIAETIRAMINTLVIDLTKTTLANISRYQPDSVGA
                     VRLAPPLAEFSASMRSQTDALKSFLLRNLYQHYQVVRMTTKARVIVRDLFVAFLNEPR
                     LLADDHRRDDGPAQARAIADYIAGMTDRFAIREHRNLFQM"
     gene            complement(2717..>3183)
                     /locus_tag="VIR04_08340"
     CDS             complement(2717..>3183)
                     /locus_tag="VIR04_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY9460794.1"
                     /translation="ALEPAAIAHAIGRSCELKAQVVSQDERDFGLRALLNFGHTFGHA
                     IEAGLGFGEWLHGEAVGCGMVQAAELSVAAAGFPAVDVARVRALVHAIGCPIKAPNLG
                     TEHWIDLMQVDKKTEAGQLRFVLLTKIGRAAVQAAPLDAVRAVLKKTAIPVL"
BASE COUNT          697 a          936 c          923 g          627 t
ORIGIN      
        1 agccatgccg ctgtcggcgt gccttgcctg gtttcaggcc ttgacgctgg ccggcgcaaa
       61 aaaggaaatt gccgaacgca tcgtgcgcga aattgaggcg cgcctgcaat ttttgaataa
      121 tgtaggcttg aattatttat cgctggatcg cagcgccgac acgatctcgg gcggagaagc
      181 acagcgcatt cgattggcca gccagatcgg ctcaggcctg accggcgtca tgtacgtgct
      241 ggatgagcca tcaatcggac tgcaccaacg cgataacaac cgcctgattg aaacactgca
      301 acatttgcgc gacttgggca atagcgtgat tgtggtcgag cacgacgagg acatgatccg
      361 gtcggccgac ttcatcgtcg acatgggccg gggcgccggc gaacacggcg gggaaatcgt
      421 ggcgcaagga tccgcccagg acattatcct caatccccgg tcactgacgg gacagtactt
      481 gagcggaaca cggcgcatcg atattcccaa acggcgtccg gtaagcccgg acgggccgtg
      541 gctaagactg aacggctgca ctggcaacaa tctgaaatcg gtgaacctgg ccattcccga
      601 cgggcgcctg gtatgcatta ccggcgtatc cggctcgggc aaatcaactc tgatcaacga
      661 cacgctggcc accgccgtcg cgcaacaact gcaacgcgct cagaccgaac ccgccccgta
      721 tcagtcgctg gagggcttgt cgctcttcga caaaatcatt aacgtcgacc aaagccccat
      781 cggccgcaca ccgcgcagca atcccgccac ttacaccggc atgtttacgc caatacgtga
      841 gcttttttcc gaggttcccg aatcgcgcac gcgcggctac ggccccgggc gcttctcttt
      901 caacgtaaaa ggcggccgtt gcgaagcctg ccagggggac ggcgtggtca aggtcgagat
      961 gcatttcctg cctgacatgt atgtgccgtg cgacctgtgc gagggcaaac gctataaccg
     1021 tgaaacgctt gaaatccgtt atcgcggccg caatatcagc gaggtcttga acctgacggt
     1081 ggaacaggcc ctgacctatt tcgaagcggt gccatccata gcgcgcaagc tcaataccct
     1141 gatggacgtg ggactgtcgt acattcgtct ggggcaaagc gccaccacct tgtcgggcgg
     1201 ggaagcgcaa cgcgtgaagc tgtctttgga actgtccaaa cgcggcaccg ggcgcacact
     1261 ttacgttttg gacgaaccca ccaccggcct gcactttcag gacatcgcaa tgctgctcga
     1321 cgtattgcgg caactggtcg attcgggaaa tacggtggtc attattgagc acaatctgga
     1381 tgtcatcaag accgccgact ggattgtgga tctggggccc gaaggcggca atggcggcgg
     1441 atatatcgtg gcgcaaggca cgcccgaaca aatagcggca aacccggaca gctataccgg
     1501 aaaatatttg cagccgctgc ttatgcacca ctaagcatgt gatgttcgtg tgccgccagt
     1561 cgctgaatcg cggccggcgt ggctacattt gaaaaagatt gcggtgttcg cgaatcgcaa
     1621 aacgatcggt cattccggca atgtagtcgg cgatcgcgcg agcctgcgcc gggccgtcat
     1681 cgcgccggtg gtcatcggca agaagacgcg gttcattcaa aaaggcgaca aacaaatcgc
     1741 gcacaattac ccgggccttt gtggtcatgc gcacgacctg gtagtgctgg tacaaattac
     1801 gcaacaggaa acttttcagt gcgtcggtct gactgcgcat cgatgccgaa aactccgcca
     1861 ggggcggcgc cagccgcacc gcgccgacgc tgtcgggttg atatcggctg atattcgcca
     1921 gtgtggtttt cgtcaaatcg atcactaatg tgttgatcat ggcgcggatt gtttccgcaa
     1981 tgacgcgacg ttgcgcaaga ccaggatatt tgcgtctgac ccgggcgcaa tgactggcga
     2041 aaatttccag ttcctgcaac tgatcaaccg tgataagacc cgagcgcaag ccatcgtcga
     2101 catcatgatt gttgtaggcg atttcgtcgg ccaaattggc aatttgcgcc tcgagcgaag
     2161 gccgtgtctt gttcaggaag cgttcgccca cgtcgcccaa cacgcgcgca tttttgacgg
     2221 agcaatgctt gagtatgcct tcacgggttt caaagcataa attcaaaccg ttaaattcgg
     2281 cataacgttc ttcaagctcg tcgaccacgc gcaaactttg caggttgtgc tcgaaccctc
     2341 ccgcatcggg agcgagttcg cgcaggcagg cgttcaattc gtcctggccc gcatgaccga
     2401 acggcgtatg ccccagatcg tgcgccagcg aaatggcttc ggtcagatcc tcgtgcaacc
     2461 ccagattgcg cgccagcgtg cgcgcaattt gcgccacctc gatgctgtgc gtcaggcggg
     2521 tgcgaaacag atcgccttcg tgattgacga atacctgcgt tttatattcg agcagacgga
     2581 aagcattgca atggataatg cgatcgcgat cgcgttgaaa ctggctgcgg ttgacgggcg
     2641 gttcttcgga atgcgcgcgg ccgcgcgatt gatcgggata acaggcgtaa gcggccagct
     2701 cggtcatagg tccggcctat aaaaccggaa tagcggtttt cttgaggacg gcgcgcacgg
     2761 cgtcaagcgg cgccgcctgc acggccgccc ggccgatctt ggtcagaagt acaaaacgca
     2821 actggccggc ctctgtcttt ttatcaacct gcatcaagtc aatccagtgc tccgtgccca
     2881 gattgggcgc cttgatcggg catccgatag catgcacaag cgcgcgaaca cgggcaacgt
     2941 cgacggccgg gaatcctgcg gcggcaaccg acagttcggc cgcctggacc atgccgcaac
     3001 ccacggcctc gccgtgcagc cattcgccga aacccaggcc cgcctcaatc gcgtgaccga
     3061 aggtatgacc gaaattcaat agcgcccgca aaccgaaatc gcgctcgtcc tgggacacta
     3121 cctgagcttt gagttcgcaa gaacgcccga tggcgtgcgc gatggcggcc ggttccagtg
     3181 ccc
//
LOCUS       DATCXU010000372         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_309810,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000372 DATCXU010000000
VERSION     DATCXU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309810"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIR04_08345"
     CDS             complement(<1..286)
                     /locus_tag="VIR04_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460795.1"
                     /translation="MSAQVMEDSAVAFTHRGKNRSWAKFRRNRSAMLGAAIVLFFLLA
                     AIFAPFVAVYDPVQVAFSSIRKAPSAVHWFGTDELGRDIFSRMVYGARASL"
     gene            complement(283..1227)
                     /locus_tag="VIR04_08350"
     CDS             complement(283..1227)
                     /locus_tag="VIR04_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9460796.1"
                     /translation="MFKTILRRVIVAIPTLILVSMLIFSLQKILPGDPVLTMAGEERD
                     PAVLTYLREKYRLNDPLPVQYLTWVREVAQGNLGVSLRTDIPVTTLIAQKLPVTAQLA
                     LMAMFFSVLVGIPLGILAAVKKGSRFEFGANLFALSGMSIPNFWLGIILIMIVAVQWK
                     LLPASGFVPPSEDLGLSIKTMLMPALVLSTAISAYLMRHTRSSMLEALRADYVRTARA
                     KGASEWVVILRHALRNALMPIVTLVTILFGELLGGAVLTEQIFGIPGFGKLLVDAVFN
                     RDYAVVQGIVLCIAAGFVTLTLVADILYVLVNPRLRYE"
     gene            complement(1295..2812)
                     /locus_tag="VIR04_08355"
     CDS             complement(1295..2812)
                     /locus_tag="VIR04_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460797.1"
                     /translation="MKKVTVWVGGFLLATAAAASFAQTLRIGMQEDPDTLDPHRSRTY
                     VSRIVFTSLCDKLVDIDAKLQFVPQLAKSWSWSPDNKVLTFKLRNDAVFHDGAKFDAV
                     AAKANLDRARTMKESQRKGELASIQQVDAPDPTTLVITVKQPDATLLAQLSDRAGMML
                     SPESFKAADSATAVGRNPVCSGPYKFVERVQNDRIVLEKFDKYYDAKDFSFKKVVFMP
                     IPDTTVRLQNLRSGSIDILERLNPSDVAQVKKDPSLVFQPITGLGFQQFMFNVSNGPQ
                     AKTNPFSNKLVRQAFQLTIDRDAINQVIGGGIFAPAQQPFPQASPYLSDKFPVTKPDI
                     AKAKALLKKAGMETVKAQLTFGNNTIASSTAEMVQAMAAQAGFQLSLRPTEYAAMLAE
                     DAKGNFQVDMRGWSGRVDPDGNIYAFVTCGGALNDGKYCNPEVDKLLKQARTVPDVTQ
                     RKAIYDQAQAILQDELPSLYTYYQPWPFVVRKSVHGFVPYPDGMIRLKGVSIAKK"
     gene            complement(2838..>4283)
                     /locus_tag="VIR04_08360"
     CDS             complement(2838..>4283)
                     /locus_tag="VIR04_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY9460798.1"
                     /translation="SCGIGGDCFAVVWDGKKLHGLNSSGVAPAVWNVDYFKRRHGTDA
                     KGLAIQPLRGWDTATVPGVVAGWSALHKKFGKLPFESLFEPAIEIAERGYAVPPVTAG
                     KWAKAAQELHSQPGFAETFMPAGHAPKIGEHFRFPDAALTLRRIAESGGRDFYEGELA
                     EKIAAFSREGGGGMTLDDLRAYQPEWVDLISKNYHGYTVHEIPPNGQGIAALMALGIV
                     ERFDLRNIPVDSTQSQHIQIEAIKLAFADLHRYVSDPRTMDVTPLQMLDSNYLDSRAG
                     LIRLDKAGSFGAGHPRTSGTIYLAAADESGMMVSFIQSNYMGFGSGVVVPHTGISLQN
                     RGVGFSMDPASPNVVGGGKRPFHTIIPGFLSKDGKPVMSFGVMGGDIQPQAHLQTLVR
                     MIDYDQQPQAACCAPRWKVNRDSTIDIESNMNADAIQGLKSLGHTFKSVYDPYMDFGS
                     GQFVWRMSEDDLEKGYVAASDSRRDGQAVGF"
BASE COUNT         1004 a         1221 c         1148 g          910 t
ORIGIN      
        1 taagggaggc ccgggctcca tacaccatac ggctgaatat atcgcgcccc agctcatcgg
       61 tgccaaacca atgcaccgcc gagggcgctt ttcgaataga ggaaaaagca acttgcaccg
      121 gatcataaac cgcgacgaag ggggcaaaaa tggccgccag taaaaaaaac aggacaatcg
      181 cggctccgag catcgcggat cgattgcgcc tgaacttggc ccatgaccga tttttcccac
      241 gatgcgtaaa tgcaaccgca ctatcttcca tgacttgcgc gctcattcgt acctcaggcg
      301 cgggttgacc agaacataca aaatatcggc aaccagtgtc agcgtgacga agcctgcggc
      361 aatacacaac acaatgccct gaaccacggc gtaatcgcgg ttaaacactg cgtcgaccaa
      421 taatttgcca aacccgggta tgccaaaaat ctgttcggtg agcactgcgc cgccgagcaa
      481 ttcgccaaac agtatggtga ccagggtcac gatcggcatc aaggcattgc gcaatgcatg
      541 gcgcaagatg acaacccatt ccgaggcgcc tttggcgcgc gcggttcgca cataatcggc
      601 gcgcagcgct tccagcatgg acgaacgcgt gtggcgcatg agataggcgc tgatggccgt
      661 cgacagcacc agggccggca tcagcatggt tttgattgac agtccaagat cttcagaagg
      721 cggcacaaag cctgacgcgg gcagcaattt ccactgaacc gccacgatca tgatcagaat
      781 aatccctagc cagaagttgg ggatggacat ccccgacaag gcgaacaaat tggcgccgaa
      841 ctcgaagcgg gatcctttct tcacagcggc cagtatgcct aatggaattc ccacgagcac
      901 cgagaaaaac atggccatca aagcaagctg agccgtgacg ggcagctttt gcgctatcag
      961 cgttgtaacg gggatatcgg tgcgcagcga aacgcccaga ttgccctggg cgacttccct
     1021 gacccaggtc aggtattgaa cgggcaacgg atcgttcagg cgatacttct cccgcaaata
     1081 agtaagcacc gccgggtcgc gttcttcgcc tgccatggtt aatacgggat ctccaggcaa
     1141 gattttctgc aagctgaaaa taagcatgga taccaatatc aaggtcggta tggccacgat
     1201 tacgcgacgt aaaatagttt tgaacatgcc tgacgccctg ctataccgga tttacaaaaa
     1261 ataggccagg cgcggagccc gaagcgccgc gcacctattt tttcgcaatg gacacgcctt
     1321 tcaggcggat catgccgtcg gggtaaggca cgaacccgtg gaccgattta cgcacgacaa
     1381 aaggccacgg ctgataatag gtgtacaggc tgggcaattc gtcttgcaag atggcctgag
     1441 cctgatcgta aatcgccttg cgctgcgtca catccggcac ggtacgcgct tgtttcaaca
     1501 gcttgtccac ttcggggttg caatatttgc cgtcgtttaa ggccccgccg caggtgacaa
     1561 atgcgtatat attgccatcc ggatcgacgc gacccgacca gccgcgcatg tccacctgaa
     1621 aattgccttt ggcgtcttcg gccagcatgg cggcgtattc agtaggtcgc aaacttaact
     1681 gaaacccggc ctgagcggcc atcgcctgca ccatttcagc ggtcgaagat gcaatggtgt
     1741 tgttgccgaa tgtcaactgc gccttgacgg tttccatgcc ggcctttttc aaaagggcct
     1801 tggccttggc gatatcgggc ttggtgaccg ggaacttatc gctaagatag gggctggcct
     1861 gcgggaacgg ctgttgcgcc ggggcgaaaa ttccaccgcc tattacttga ttaatggcat
     1921 cgcggtcgat ggtcagttgg aaagcctggc gaacgagctt gttgctgaat ggattcgtct
     1981 tggcttgtgg cccattgctg acgttgaaca tgaactgctg aaagcccagt cccgttatgg
     2041 gctggaaaac cagcgacgga tcctttttaa cttgggcgac gtcggacgga ttcagacgct
     2101 cgagaatatc aatgctgccc gaacgaaggt tttgcaaacg caccgtcgtg tcgggaatgg
     2161 gcatgaacac cactttctta aatgaaaaat ctttggcgtc gtaatacttg tcgaattttt
     2221 ccaggacaat gcgatcattt tgcacgcgct cgacaaattt gtagggcccc gaacaaaccg
     2281 gattgcggcc gacagcggtt gcgctatcgg ccgctttgaa actttcgggc gacagcatca
     2341 tgcctgcgcg atccgacaac tgcgccagca atgtcgcatc aggctgtttt accgtaatga
     2401 cgagcgtcgt ggggtcgggc gcatcgactt gctgaattga agccagttcg cctttgcgct
     2461 ggctttcctt catggttctg gcgcgatcca ggttggcctt ggccgccacc gcgtcaaact
     2521 tggcgccgtc atgaaaaacg gcatcgttgc gcagcttgaa tgtcagaacc ttattgtccg
     2581 ggctccagga ccaggacttg gcaagttgcg gaacgaattg caacttggca tcgatatcga
     2641 ccagcttgtc gcataaagac gtaaatacaa tgcgcgaaac ataagtgcgc gagcgatgag
     2701 gatcgagcgt gtcggggtct tcttgcatgc caatacgcaa ggtctgcgca aaactggccg
     2761 cggcggccgt cgccagcaag aagccgccca cccaaaccgt aacttttttc atattttagt
     2821 ctcctgattt tgttatttta aaaaccgact gcctgaccgt cgcgccggct atcgctggcg
     2881 gcaacgtatc ccttttccaa gtcgtcttcg gacatgcgcc aaacgaattg gcccgatcca
     2941 aaatccatgt acggatcata aaccgatttg aacgtatgcc ccaaagactt cagcccctga
     3001 atcgcgtcgg cattcatatt cgactcgatg tcgatggtgg aatcgcggtt cactttccag
     3061 cgaggcgcgc aacatgccgc ttgcggctgc tgatcataat cgatcatgcg cacgagtgtc
     3121 tgcagatgag cctgcggttg aatgtcgccg cccatgacgc caaagctcat gactggcttg
     3181 ccatctttgc tcaggaatcc gggaataatc gtatggaagg gacgcttgcc gccacccact
     3241 acattcggcg acgcaggatc catggagaat ccgacgccgc gattttgcag actgatccca
     3301 gtatgcggca ccacgactcc cgagccgaaa cccatgtaat tcgactggat gaatgaaacc
     3361 atcatgccgc tttcgtcggc ggccgccaga taaatagtcc cgctcgtacg cggatgcccg
     3421 gccccgaaag aaccggcttt gtccagccgg atcagcccgg cccggctatc cagataattc
     3481 gagtcgagca tttgcaaagg cgtaacgtcc attgtgcgcg gatctgacac atagcgatgt
     3541 aaatcggcaa aggccagttt tatcgcttcg atttgaatgt gctgcgactg cgtagagtca
     3601 accgggatgt tgcgcaaatc aaagcgctcg acaatgccca aagccatcag ggccgctatg
     3661 ccctgaccgt tcggagggat ctcatgcacg gtgtagccat ggtagttttt ggaaataagg
     3721 tcgacccatt cgggttgata agcccgcaga tcgtccaggg tcatcccgcc accgccttcg
     3781 cggctgaacg ccgcgatttt ttctgccaat tcgccctcat aaaagtcgcg accaccactt
     3841 tcagcaatgc gccgcaacgt taaggctgca tcaggaaaac ggaaatgttc cccaatcttt
     3901 ggagcgtgtc ctgcaggcat gaacgtttcg gcaaaccccg gctgactgtg cagttcttgt
     3961 gccgccttgg cccacttgcc cgccgtcacc ggcggcaccg cgtagccgcg ttcggcaatt
     4021 tcaatcgcgg gctcgaacaa agattcaaac ggcaacttgc cgaatttttt atgcagagcg
     4081 gaccagccgg caaccacacc aggcaccgtt gcggtgtccc agccccgcag gggttggatt
     4141 gccagacctt tcgcatcggt cccatgcctg cgcttgaaat agtccacgtt ccatacggcc
     4201 ggagccacgc ccgacgagtt caagccatgc agctttttac cgtcccatac taccgcgaag
     4261 cagtcgccgc caatcccgca gga
//
LOCUS       DATCXU010000373         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_114372,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000373 DATCXU010000000
VERSION     DATCXU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114372"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1768
                     /locus_tag="VIR04_08365"
     CDS             167..1768
                     /locus_tag="VIR04_08365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY9460799.1"
                     /translation="MTLPCPSLLSDRAQLMATLSRKKNVDLVVVGGGASGLGVALDAT
                     LRGLSVVLLESLDFAKGTSSRATKLVHGGVRYLAQGNIALVREALHERQTLLRNAPHL
                     ARPLPFVMPSYKFWEKPFYGLGLKFYDALAGKAGLGPTEFLSAGKTALRLPGVKTQGL
                     RGGVQYWDGQFDDARLALALARTAAAQGALLVNYCPVTGLVHDRHRVTGVRCQDAETG
                     MSYQIRARCVVNATGVWVDELRQKDGAAIHRPTEPMVAPSQGVHLVVDREFMPSNHAL
                     LVPKTQDGRVLFVVPWLGKLILGTTDTPRHDVSREPDAFAEEVEFILSEAGKVLTHAP
                     ARSDVRSIWVGLRPLVKPVGDEAHLNNTKKISREHTVLVSASGLVTVTGGKWTTYRAM
                     AEDVLEKCGIAGLLPDLPASRTSDFPLVGAAPAGGGRTTLTQAPGLDAYGSEGDCVQS
                     LQGCDRVLTDGLTEAMVRFGARYEYARCVEDILARRSRLLFLDARLAAQLAPEVARIM
                     SEETGIDPKLDEFLRLAAHYLKLPC"
     gene            complement(1765..>2188)
                     /locus_tag="VIR04_08370"
     CDS             complement(1765..>2188)
                     /locus_tag="VIR04_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY9460800.1"
                     /translation="NANSRPPIIISDDKREEVVLTALALLDTRYKYGGTTPARGFDCS
                     GFVTYVFQYASDNTLPHNTASIARISRPIPENGLRPGDFVFFNTLGQPYSHMGIYIGD
                     GRFVNAPSSRSGGRVRIDSLRDPYYAQRFVDARTLFRL"
BASE COUNT          442 a          562 c          699 g          485 t
ORIGIN      
        1 aaggccagca aataggtcat ggttatgcgg tgacaaaaaa tttaggccga aaatcgaaaa
       61 gccggtcaga caaagtgata aaagattagc cgaaaaggtt gatcgacgat agcatgaatt
      121 ggcgtcataa acccctgatc gagtacgccg ccttatactg atgcgcatga ctttaccttg
      181 cccgtcatta ctttccgacc gtgctcaatt gatggcgact ttgtcgcgca aaaaaaatgt
      241 ggatctggtg gtcgtcggag gcggcgcttc gggtttggga gtggcacttg acgcaacgtt
      301 gcgcggactg tcggtggttc tgctggaatc gcttgatttc gccaagggca cctcgtcgcg
      361 cgccaccaag cttgtccacg gcggcgtccg ttatctggcg caaggcaaca tcgcgctagt
      421 gcgcgaagcc ttgcatgaac gacagacttt gctgcgtaac gcgccgcacc tggcgcggcc
      481 cctcccgttt gtcatgccca gctataagtt ttgggaaaag ccgttttatg gcctgggctt
      541 gaaattttat gacgcgctgg cgggcaaggc cggtctgggc cccactgaat ttctaagcgc
      601 cggaaaaacg gcgctgcgcc tgcctggcgt taagacccag ggtttgcggg gcggtgttca
      661 atattgggat ggtcagttcg acgatgcccg ccttgcgttg gccttggcca ggacggctgc
      721 cgcgcagggc gcgctgctgg tcaattactg ccccgtgacg gggctggttc acgatcggca
      781 cagggtgacc ggcgtgcgtt gccaagatgc tgaaaccggc atgagctacc agattcgcgc
      841 ccggtgtgtt gtgaatgcga ctggtgtttg ggtggacgaa ctgcggcaaa aggacggcgc
      901 ggcaattcat cggccaactg aaccaatggt ggcgcccagt cagggggtgc atttggtcgt
      961 ggaccgtgaa tttatgccgt cgaaccacgc tttgctcgta cccaagacgc aagacggccg
     1021 ggtgttgttt gtggtgccat ggctgggtaa gctgatttta ggaaccactg acacgccgcg
     1081 ccacgacgta tcgcgcgaac ccgacgcgtt tgccgaagag gtcgagttta ttctgagcga
     1141 ggccggcaaa gtattgacgc atgcgccggc gcgttccgat gtgaggagta tttgggtcgg
     1201 gttgcgtcct ttggtcaagc ccgttgggga cgaggcgcat ctcaataaca ccaaaaaaat
     1261 tagccgtgaa cataccgtac tggtcagcgc gagcggtctg gtcacggtta ccggtggcaa
     1321 gtggaccacc tatcgggcga tggccgagga cgtgctggaa aaatgcggga tagcagggtt
     1381 attgcctgat ttgcctgcaa gccggacaag tgacttccct ctggtgggag cggcgcccgc
     1441 cggcggtgga cgcacaacct tgacgcaggc tcccggtctg gatgcgtatg ggtcggaagg
     1501 cgattgtgtt caaagcctgc agggttgcga tcgcgtattg acggatggac tgaccgaggc
     1561 catggtgcgt ttcggggcgc gttatgagta cgcccggtgc gtggaggaca ttctggcgcg
     1621 gcgctcccgg cttttgtttc tggacgcgcg tctggccgcg cagcttgctc ctgaagtggc
     1681 acggatcatg agcgaagaaa cgggtattga tcctaaattg gatgaatttt tacgacttgc
     1741 cgcccactac ctgaagttgc cctgttagag ccggaacaaa gtcctcgcgt caacgaaacg
     1801 ttgcgcgtag taggggtcgc gcagcgaatc gatgcgaacc cggccgccgc tgcggctgga
     1861 tggcgcatta acgaaacgtc catcgccgat atagatgccc atatgtgaat aaggttggcc
     1921 cagtgtgttg aaaaacacga aatctcctgg gcgcaatccg ttttcgggaa tcgggcggct
     1981 gatccgggcg atacttgcgg tgttatgcgg caacgtgttg tcgctcgcgt attgaaaaac
     2041 ataggtcacg aagccgctgc aatcgaaacc gcgcgccggt gttgttcctc cgtatttata
     2101 acgtgtgtcc agcaacgcca atgccgtgag gacgacttcc tcgcgtttgt cgtcgctgat
     2161 gattatcggc gggcgtgaat tggcgttc
//
LOCUS       DATCXU010000374         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_587690,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000374 DATCXU010000000
VERSION     DATCXU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587690"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VIR04_08375"
     CDS             <1..398
                     /locus_tag="VIR04_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEY9460801.1"
                     /translation="VPLAVKIAPDLSTEQIDIIADILPRYGVDGVIATNTTLARDAVK
                     GLPYAEQAGGLSGPPVHALSLAVIARLRHQLGASFAIIGVGGIMSGKQAQEKIAAGAN
                     AVQLYTGLIYRGPALIDECVRALAQTPDH"
     gene            complement(511..1086)
                     /gene="phaP"
                     /locus_tag="VIR04_08380"
     CDS             complement(511..1086)
                     /gene="phaP"
                     /locus_tag="VIR04_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525805.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY9460802.1"
                     /translation="MSAVPQQVFANQKASLDGFLAVQGSVFGAFEKLVDLNLKVIRAT
                     LDEVAEKSQQAIEVKDAQEALAFTSALVQPGAEKALAYGKHVYDIVTGVQADLSKLTE
                     EQIALGQQHVSNAIDQFSKNAPTGSESAVALLKSSLATAASAYESVAKAAKQATEVAE
                     SNITAATNATFKAASDAADVAKAPRARRSAA"
     gene            complement(1355..2158)
                     /locus_tag="VIR04_08385"
     CDS             complement(1355..2158)
                     /locus_tag="VIR04_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY9460803.1"
                     /translation="MSFAPSSGALPHINGSLITVQVLERAMLLLDVLAAQPEPVALKD
                     LAATTGLHNSTAHRILNDLVVGRYVERVDSGLYRLGMRLLELGSLVKGRLNVREAAID
                     PMRELHKITGQTVNLSLRQGDEIVYIERAWSERSGMQVVRAIGGRAPLHLTSTGKLFL
                     SVSDPRQIKAYIMRTGLAGNTQNSITQADELERDLARVRRHGYSRDNEELELGVRCIA
                     AGIYDDTGKLQAGLSISAPAERMRDEWIAALLTTASRISVSLGYEAAPD"
     gene            complement(2231..3037)
                     /locus_tag="VIR04_08390"
     CDS             complement(2231..3037)
                     /locus_tag="VIR04_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY9460804.1"
                     /translation="MSTFVTPRVRLLIIGDEILSGRREDKHFVKMVQLLSQRGMQLSG
                     AEFISDDRSTIAATLRRTFASGDIVFSCGGIGATPDDQTRQAAAEALGVELQLHPEAE
                     RLIAERCADSASRGQGSADMSLPENQQRLQMGRFPAGVEIVPNPYNKIPGFFIKDHTF
                     MPGFPVMAWPMIEWTLDTRYRKFHHSVTRVEQSFLAFRLPESRITPALEELEKKWPGV
                     KAFSLPSMGETGHPHIDLGVKGEPAAAAQALAYLRAEVIRVGGSLTPPEK"
BASE COUNT          725 a          917 c          842 g          680 t
ORIGIN      
        1 ccgttcctct ggccgtcaaa atcgcccccg acttaagcac tgagcaaatc gacatcatcg
       61 ccgacatctt gccgcgctac ggggtcgatg gcgtaatcgc caccaacacg accctcgcac
      121 gcgacgccgt gaagggattg ccgtacgcgg agcaggcggg aggtctatcg ggcccgccgg
      181 ttcacgcgct gtcgcttgcc gtcattgccc gtttaagaca tcaactgggt gcatcgtttg
      241 ccatcatcgg cgtgggcgga attatgtccg gcaaacaggc tcaggaaaaa atcgcagcag
      301 gcgccaatgc ggtgcagtta tataccggct tgatttaccg cggcccggcg ctgatcgacg
      361 agtgcgtccg agccttggcc caaacgccag accattaaaa aaccccaaag aaaagcggct
      421 tctttggggt ttaaagcgta agacctctac ggtgattcgc tattcacttt aacatggcgg
      481 cgcttgtcac gccgccatgg gccagcacta tcaggcggcg ctacgacggg cgcgaggcgc
      541 cttggcgacg tcagccgcgt cagaggctgc cttgaacgtc gcgttggttg cggcggtgat
      601 gttcgattcc gctacttcag tcgcttgctt ggccgctttg gcaaccgatt cataggcact
      661 ggcggcggtc gccaaggacg acttcagtaa cgcaacagcg ctttcagagc cagtgggcgc
      721 attcttcgaa aactgatcga tcgcgttcga tacatgttgc tggcccaggg caatttgctc
      781 ttcggtcagt ttggacaaat cggcctggac acccgtgacg atatcgtata cgtgcttacc
      841 ataggccagc gctttttcag cgcctggctg aacaagagcc gaggtaaagg ccaaagcttc
      901 ttgcgcgtct ttgacttcaa tggcttgctg cgatttttcg gccacttcat ccagcgtggc
      961 gcggataact ttcagattca aatcgacgag tttttcaaag gcgccgaaaa ccgagccctg
     1021 aacagcaaga aagccgtcga gcgatgcttt ttgattggcg aaaacttgtt ggggaactgc
     1081 gctcatggta tttccttcat aaatgaccgg tgtttatttt gccggtcgaa acaattgaac
     1141 caggttcaat ctaaatttaa gaaggttaag caaatcaacc atactccgtg acaaccttct
     1201 cgtatttgct gcactgcaca acttcaattc taccttaaat accagtgctg tcaactcgtt
     1261 tttgctgcga cgcaacataa atcggaaaaa cagggaattt gtggtgtttt tgccgcttcg
     1321 ccaacctgaa gacggcgagg ccggccccgg cagcctagtc aggcgccgcc tcgtaaccca
     1381 gcgaaacgga tatccgcgaa gctgtggtca ataacgccgc gatccattcg tcccgcatgc
     1441 gctccgcagg tgcggaaatg gacaagcccg cttgcagctt tccagtgtca tcgtaaattc
     1501 ctgctgcaat gcaacgcacg ccgagttcaa gctcttcatt atcgcgagaa tacccgtgac
     1561 gccggacccg cgccagatcg cgttcgagtt catcggcttg ggtgatgctg ttttgggtgt
     1621 tgccggccag gccagtgcgc atgatgtatg ctttgatctg acgcgggtcg ctaaccgata
     1681 aaaataactt gcccgtggac gtcaaatgca acggcgccct gccgcctata gcgcgaacaa
     1741 cctgcattcc cgagcgctcg ctccaggctc gttcgatata tacgatctcg tcgccctggc
     1801 gcagcgacaa attgacggtc tgacctgtta ttttatgcag ctcgcgcatg ggatcgatgg
     1861 ccgcttcgcg tacattcaag cgccctttca ccaacgagcc cagttccagc aaacgcatgc
     1921 ccaggcgata caagccgcta tccacgcgtt caacataacg gcccacaacc aggtcgttca
     1981 aaatgcgatg cgctgtggaa ttgtgcaatc ccgtcgtcgc agcaagatct ttaagcgcaa
     2041 ctggctcggg ctgagcggcc aatacgtcaa gcaacaacat ggcgcgctcc agcacctgaa
     2101 ccgtgatcaa cgatccgtta atgtgcggca acgcgcctga ggaaggagca aatgacatgg
     2161 agcccggcat aacctgtatg atgcaacgca acaattccat catatgaaaa aaatttcgat
     2221 ggcgcaagcc ctatttttca ggcggggtta acgaccctcc gacacgaatc acctctgccc
     2281 gcagataggc cagcgcctgt gcagcggcgg caggctcgcc cttcacaccc agatcaatat
     2341 gcggatgccc cgtttcgccc atgctgggca ggctgaatgc ctttacgccc ggccattttt
     2401 tttcgagttc ttcaagtgcc ggcgtgatgc gcgactcggg cagcctgaac gccagaaacg
     2461 attgttcaac gcgggtgacg ctatggtgaa actttcgata gcgggtatcg agtgtccatt
     2521 cgatcatggg ccaggccatc accggaaatc ctggcataaa cgtatggtct ttgataaaga
     2581 atcccggaat tttgttgtaa gggttcggaa cgatctcgac acccgccgga aaccggccca
     2641 tctgcaaacg ctgctgattc tcgggcaagg acatgtcggc gctgccctgg ccgcggcttg
     2701 cactgtcggc gcaacgttcg gcaatcagac gctcggcctc gggatgcaac tgaagctcaa
     2761 cccccagcgc ctccgccgcc gcctgacgcg tttgatcatc gggagtagcc ccaatgccgc
     2821 cgcaactgaa gacgatgtcg ccgctggcga atgtgcgccg caatgtagcg gcaatggtgg
     2881 agcgatcatc agagatgaac tcggcgccgc ttaactgcat gcctcgctgg gataaaagct
     2941 gaaccatttt gacaaaatgt ttgtcctcgc ggcgccccga taaaatttca tcgccaatga
     3001 tcagcaagcg cacacgcggc gtaacgaaag tactcatctc gaaccttgtt caataaaagc
     3061 taagcattaa tgaccgtttc ccggcgcaat tgcctgagcg cctgaagact gaaatgggca
     3121 aataccaaag ccgaaaacac cggtagcacc agccaggcag gcgg
//
LOCUS       DATCXU010000375         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_191467,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000375 DATCXU010000000
VERSION     DATCXU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191467"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VIR04_08395"
     CDS             complement(<1..436)
                     /locus_tag="VIR04_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859634.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460805.1"
                     /translation="MNYTFQFGPVFDAWPLLLKGTWITIQLSFLAMLFGLMVAVVCAW
                     VKTSGPAILRWLVNGYIEVIRNTPFLVQLFFFFFALPEAGVRWTAHTAALVAMVVNLG
                     AYATEIVRAGIQAIPREQIEAGQALNLSRLEIFRFIILKPALR"
     gene            604..2064
                     /gene="zwf"
                     /locus_tag="VIR04_08400"
     CDS             604..2064
                     /gene="zwf"
                     /locus_tag="VIR04_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY9460806.1"
                     /translation="MATTPIFDIIFFGGTGDLALRKLLPALYHAHKAGLLHEQGRIFA
                     LGRGDLGQDGYMSLLDERVRPRLGAEFDEQTWALFAKRIHFGRLNASNPQDYATLVRD
                     LGGLSERITICYLATAPHLFAIICEQLAAVGLNHANVRVVLEKPLGHDLASSNAINAA
                     VGQCFTENQIYRIDHYLGKESVQNLMAIRFGNVLFEPLWRREWINDVQITIAEELGVE
                     ERGEFYDQTGALRDMVQNHLLQLLCIVAMEPPVSMAEDAVRDEKLKVLKALKPFTNES
                     VVVKTVRGQYRAGAINGLPVAGYREEHNVAADSNTETFVALQADIANWRWAGVPFFLR
                     SGKRMQERVTEIVINFRNVPHAVFSMPAGLHAANRLVISLQPKESVRLYFLAKEPGDG
                     MNLRPTYLNLDFHATFKARWADAYERLLLDVIRGRLALFMRRDELAQAWCWVEPIIDS
                     WQQFSIAPKPYTAGSWGPSASSALLSRMGVLWHEET"
     gene            2067..2741
                     /gene="pgl"
                     /locus_tag="VIR04_08405"
     CDS             2067..2741
                     /gene="pgl"
                     /locus_tag="VIR04_08405"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY9460807.1"
                     /translation="MWHAFDANAPYINALGNTISARLQRTIESNGQACIAVSGGRSPI
                     ALFDRLSRCDINWEKIHVTLVDERFVAPDDEDSNERLVRDHLLVNQARRAKFTGLVTD
                     PASILNCVERANQAIQSVNLAILGMGDDGHTASLFPGAPQLAEALSLEQTHRYMRITP
                     PAAAHERISMTLAALLHAEQLILAISGPHKRKIYEQAAQKRTPELPVSFLITQSLAPL
                     DVYWHA"
     gene            2746..>3518
                     /gene="glk"
                     /locus_tag="VIR04_08410"
     CDS             2746..>3518
                     /gene="glk"
                     /locus_tag="VIR04_08410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY9460808.1"
                     /translation="MPNPGWNLVGDVGGTHARFALCKDASALEKISVLDSADFAGIDS
                     AVKHYLGLHGQPKINHAAIGMANPVTGDHVTMTNLPWAFSTEETRNALGLNSLHIIND
                     FAALALAVPLLSPAGFIQMGGQSAVPDTPLAVLGPGTGLGISALVPTRDSGWAVIAGE
                     GGHADFAPGNERELTLWREAQRKFGHVSKEHLLSGPGLEFIYQIFSRLSGKPMQTRSA
                     AEISAQAISNSCAVCRESLDTFCAILGAVAGDMALTLGAR"
BASE COUNT          797 a         1048 c          958 g          715 t
ORIGIN      
        1 ctcgcagtgc cggcttcaat atgatgaaac ggaaaatttc cagacgcgac aggttaagcg
       61 cctggcccgc ttcaatttgt tctcggggta tagcctgtat gccggcgcgc acgatttcag
      121 ttgcgtaagc cccaaggttg accaccatgg caaccagtgc cgcggtatgc gccgtccatc
      181 gaaccccggc ttcgggcaac gcgaaaaaaa agaagaaaag ttgcaccagg aacggcgtgt
      241 tgcggataac ttcaatataa ccattgacaa gccagcgcaa tatggccggc cccgaggttt
      301 taacccacgc gcacacaacc gcaaccataa gcccgaacag catcgccaga aacgataact
      361 gtatcgtgat ccatgtgccc tttaggagca gcggccaggc gtcgaaaacc ggaccaaact
      421 gaaaggtgta attcacgtat tagtgcgccg ccaaaattgc catcaggcaa tgataagcag
      481 ttaggtcaaa attagcgata actttgaatt attaccaata gattcaagac cggtaaaacg
      541 aaaaacaaac tcacgaggaa ataataggcg gtattatgaa gcggttccaa aaaggatcct
      601 gcgatggcga ccacacccat cttcgatatt atattttttg gcggcacagg cgacctggcc
      661 ttgcgcaagc tgttgcccgc gctctaccac gcgcataagg ccggactgct gcatgaacaa
      721 ggtcgcattt ttgccctggg gcgcggcgat ctcgggcaag acggttatat gtcgctactt
      781 gacgaacgtg tcaggcccag gctgggcgcc gagttcgacg aacaaacatg ggctcttttt
      841 gccaagcgca ttcatttcgg ccggctcaat gcctcgaacc cgcaggacta tgccacgctg
      901 gtccgggatc tgggcggcct gagcgagcgc atcacgattt gctacctggc cacggcaccg
      961 catctgtttg ccattatttg cgagcagctt gccgccgtcg gactaaacca tgcgaatgta
     1021 cgcgtcgtgc ttgaaaagcc gctgggccac gacctagcct cgtccaacgc catcaacgcc
     1081 gccgtgggcc agtgcttcac cgagaaccag atttatcgca ttgaccacta tcttggcaag
     1141 gagtcggtgc aaaacctgat ggccatacgc tttggcaacg ttctgttcga accattgtgg
     1201 cgccgtgaat ggattaacga tgtccagatc accatcgccg aggaactggg tgtcgaagaa
     1261 cgcggcgaat tctacgatca aaccggcgcc ttgcgcgata tggtgcaaaa ccacttgctg
     1321 caattgttat gcatcgtcgc gatggagccg ccggtcagca tggcggaaga tgctgtccgc
     1381 gacgaaaagc ttaaagtgct taaggcgctg aagcccttca caaacgagag cgtcgtcgtc
     1441 aaaaccgtac gcggccaata ccgcgcaggc gccatcaacg gtctgcccgt ggccggctat
     1501 cgtgaagaac acaacgtagc cgccgacagc aacaccgaga cgttcgtcgc cttgcaggcc
     1561 gacattgcca attggcgctg ggctggcgtg cctttctttt tgcgttcagg caaacgcatg
     1621 caggaacgag ttacggaaat tgtcattaat ttccggaatg tcccccatgc cgttttctcc
     1681 atgccggcgg gcctgcacgc cgccaatcgt ctggtcatca gcctgcaacc caaggagagc
     1741 gtgcgccttt atttcctggc gaaagaaccc ggggacggga tgaatctgcg ccccacctac
     1801 ctgaatcttg attttcatgc aacgttcaag gcgcgttggg ccgatgctta cgagcgtttg
     1861 ctgctggatg taatacgggg acgtcttgct ctattcatgc gccgcgacga actcgctcaa
     1921 gcctggtgct gggtcgaacc catcatcgat tcgtggcaac aattctccat cgcaccaaaa
     1981 ccctacaccg ccggaagctg gggaccttcc gcatcaagcg ccttgctttc acgcatgggc
     2041 gttctctggc acgaggaaac ctgatcatgt ggcacgcatt cgacgcaaac gcaccctata
     2101 tcaatgccct tggcaacacc atttcggccc gtctgcaacg caccatcgaa agcaacgggc
     2161 aggcgtgcat cgccgtgtcg ggcgggcgtt cccccattgc gcttttcgat cgtctaagcc
     2221 ggtgcgacat caactgggag aagatccacg ttacgctggt cgacgagcgg ttcgtcgcgc
     2281 ctgacgacga ggacagcaat gaacgactgg ttcgtgacca cctgctcgtc aaccaggcgc
     2341 gccgggccaa attcacggga ctcgtcaccg accctgccag catactgaac tgcgtggaac
     2401 gggccaacca ggcaatccag tctgtgaatc ttgccattct gggaatgggc gacgacggcc
     2461 ataccgcctc gctgtttcct ggcgctcccc agcttgccga agctctgagc ctcgagcaaa
     2521 cgcatcgtta catgcgaatc acgccgcccg ccgccgccca cgaacgcatc agcatgacgt
     2581 tggcggcttt actgcatgcg gagcaattga tactggccat atcagggccg cacaagcgga
     2641 aaatctacga gcaggccgcg caaaaacgca cacccgaact tccagtcagt tttctaatca
     2701 cgcaaagcct ggcgccgctt gacgtctatt ggcatgcgta agcccatgcc caatcccggc
     2761 tggaatctgg tgggagacgt gggcggcacg catgcacgct ttgccttgtg caaagatgcc
     2821 agcgcccttg aaaaaatcag cgtccttgat tcggcggatt ttgcaggcat tgactctgcc
     2881 gtcaagcact acctgggctt gcatgggcag cccaaaatca atcacgcggc cattggcatg
     2941 gcgaatccgg ttaccggcga tcacgtaacc atgaccaact tgccatgggc attttccact
     3001 gaagaaaccc gcaacgcgct gggtctgaat agcctgcaca tcatcaacga ttttgcggca
     3061 ctggctctgg ctgtccctct tttatcgccg gccgggttca ttcaaatggg cggtcaatcg
     3121 gccgtgcccg atacgccgct ggccgtcctt ggaccgggca ccggcctggg catatcggct
     3181 ttggttccca ctcgggatag cggttgggcg gtgatcgccg gcgaaggagg acacgcggat
     3241 ttcgcccccg gcaatgagcg ggaattgaca ttgtggcgcg aagcgcaacg caaattcggc
     3301 cacgtttcca aagagcactt gctgtccggg ccggggctcg aattcatcta tcaaattttc
     3361 agccgtttgt ccggcaagcc aatgcaaaca cgctccgcgg ccgaaatcag cgcccaggcc
     3421 atcagcaatt cctgcgcggt ttgccgcgaa tcgctggaca cattctgcgc catacttgga
     3481 gcggtggcgg gcgatatggc gctaactctg ggcgcccg
//
LOCUS       DATCXU010000376         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1150137,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000376 DATCXU010000000
VERSION     DATCXU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150137"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..605)
                     /locus_tag="VIR04_08415"
     CDS             complement(48..605)
                     /locus_tag="VIR04_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019076 - viral release from host cell
                     [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HEY9460809.1"
                     /translation="MKWATGAINFLRKNWLDLALLFLALSFLAVIYFTGVSNGRHARD
                     LEAKAELARISESHTQALLAAADTARRMEKHYTQQMALIDEKHTKELNNEKAMADSTI
                     TALRAGAIRMRDRFTCAAGTGLRLPQVGTTAGMGDGATAGGLQIEDGTFLIREAERAN
                     KVATQLAACQAIVKSDRDGKNHASF"
     gene            complement(602..1093)
                     /locus_tag="VIR04_08420"
     CDS             complement(602..1093)
                     /locus_tag="VIR04_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HEY9460810.1"
                     /translation="MPRISVDAAGGLNVCAFLDMLAWSEGTSTMKGSDDGYNVIVGHT
                     LFPATPAGYADHPRILVPLQKLGIKSSAAGRYQVLARYFDHYKKELRLPGFDPVSQDC
                     IALRQIKEERAFGAVLEGDFERAVYLVKNIWASLPGAGYGQHEHKLDALLGVFCLHGG
                     EPA"
     gene            complement(1094..1411)
                     /locus_tag="VIR04_08425"
     CDS             complement(1094..1411)
                     /locus_tag="VIR04_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460811.1"
                     /translation="MLPLLNGMAALALLLWASWCVFSNHVNDGILGKAIYSATAISAL
                     ALLLCPPYTEHRAPAAEVTLNLCMAALAIRHYWLKKCWPFIQVKCGTRKCAPGLQNEF
                     GDL"
     gene            complement(1411..1737)
                     /locus_tag="VIR04_08430"
     CDS             complement(1411..1737)
                     /locus_tag="VIR04_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460812.1"
                     /translation="MEWLHRLATQLATQMEWGVAGFIGAMVGAWAQRKEMRTRFEFAA
                     FMGTGAACAYFLTGLVAGYFSIEPANAGAVGFLLGAFGGSMIAAIVRAIQAADLWALI
                     KSKVGR"
     gene            complement(1721..2173)
                     /locus_tag="VIR04_08435"
     CDS             complement(1721..2173)
                     /locus_tag="VIR04_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460813.1"
                     /translation="MQLHRTVREIADVIGRDRALFLVGQLPRCYSGIQGKKSWRAILY
                     VPKSLKPNHPLVRILGWQDAEKLVRAFGGEILQPANCADIYRRFRDSSICRMAGAGMK
                     AACIASVMEVSERHVKNVLRERPQEETATPRPDTARHRSTGVSHGMAA"
BASE COUNT          562 a          745 c          692 g          625 t
ORIGIN      
        1 ggagcaatgc ccccgcacgg tacggccccc tcggaagtac cttcgcctta aaagctggca
       61 tgattctttc catccctatc actcttgaca atagcctgac aagctgctag ttgcgtcgct
      121 actttgttgg ctcgctcggc ctctcgaata agaaatgttc catcttcaat ctgaagtccg
      181 ccggctgttg ctccatcacc catgccggcg gtggtgccaa cttggggcag tcgtaggccg
      241 gtgcctgctg cacaggtgaa acggtcgcgc atcctgatgg caccagcacg caaagcagta
      301 atagtgctat cagccatagc tttttcattg ttcaattcct ttgtatgttt ttcatctatc
      361 aatgccattt gctgggtgta gtgcttttcc atacgcctgg ctgtatctgc tgcagcgagt
      421 agggcttgtg tatgtgattc actgattcgc gccaactcgg ccttggcttc caggtcgcgc
      481 gcatgcctcc cgttgctaac cccagtgaaa taaatgacgg ccaggaatga aagcgccaaa
      541 aacaacagcg ccaggtcaag ccagtttttg cgtaaaaaat tgattgcccc agttgcccat
      601 ttcatgccgg ctcacctcca tgtagacaga aaacgcccag cagggcgtcc agcttgtgtt
      661 cgtgttgtcc atagccagcg ccaggaaggc tggcccatat gtttttcacc agatagacgg
      721 cgcgctcgaa atcgccctct aacacagcgc caaatgcgcg ttcctcttta atctgacgca
      781 gcgcaatgca gtcttgcgaa accgggtcaa acccaggcaa gcgcagttct tttttgtaat
      841 ggtcgaagta gcgcgccagc acttgatagc ggccggcggc gcttgacttg atgcccagct
      901 tttgcagcgg caccaggatg cgaggatgat ctgcatatcc agccggcgtt gcagggaaca
      961 gcgtatggcc cacaatcacg ttgtatccat cgtcgctgcc cttcatggtg ctggtccctt
     1021 cggaccaggc caacatatcc aggaatgcgc aaacattcag accgcctgcg gcgtctacgc
     1081 tgatgcgagg catctacagg tcgccaaact cgttctgcag gccaggtgcg catttccgtg
     1141 tgccgcattt cacctgtata aaaggccagc atttttttag ccagtagtgg cgaatggcca
     1201 gggcggccat gcacaggttt agcgtgactt ctgcggccgg cgctctgtgt tcggtgtatg
     1261 gcggacaaag cagtaaggcc agggcggaaa ttgccgtggc cgaataaatc gctttcccca
     1321 ggatgccgtc attcacatgg ttagagaaaa cgcaccagga agcccacaac agcagggcca
     1381 gggcggccat accatttagc aagggaagca ttagcggccc acctttgatt taatcagcgc
     1441 ccacaggtct gctgcctgta ttgcgcgaac gattgcggca atcatcgacc caccgaaggc
     1501 gccaagcagg aatcccacgg cgccggcgtt ggctggttca atgctgaaat agccggcgac
     1561 aaggccggtg agaaaatagg cgcacgcggc gccggtgccc atgaaagctg cgaactcaaa
     1621 ccgcgtccgc atttccttgc gctgcgccca tgctccaacc attgcgccga taaagccggc
     1681 cacaccccat tccatttgcg tggccaactg cgtggccaat ctatgcagcc attccatgcg
     1741 aaaccccggt cgaacgatgc cgcgcagtgt cgggcctggg cgtcgctgtt tcctcttgcg
     1801 gtctttcccg caatacgttt ttgacgtgtc tttctgacac ttccatgaca ctcgcaatac
     1861 aggccgcttt catcccggcg ccggccatcc tgcaaatgct cgaatccctg aagcgccggt
     1921 aaatgtcggc acagttggcc ggctgcagga tttcgccgcc gaatgcccgg accagctttt
     1981 ctgcgtcttg ccacccaaga atgcggacaa gcgggtgatt tggcttcagt gacttgggaa
     2041 cgtacagaat ggcgcgccat gattttttcc cctggatgcc cgaatagcag cgcggcaatt
     2101 gaccaaccag gaataacgcc cggtccctgc ctatcacgtc cgcaatctcg cggactgttc
     2161 ggtgcaattg catctgttcc cctggcttgt catttttact tctgtaagta aattctactt
     2221 gctgcagtgt cgtgcaggcc agtcattttt gcgacgcgac gtgatctagt gacaaagtga
     2281 cagaaatgac acccgtttct aaagatatac ggggctatag gacaagggca tataggtgcc
     2341 tttatgttct atatttttgt cactttagtc acttagtcac taatactata taaatcaata
     2401 gattatattg tgactatctt ttataaaagg tgactatctc agacacatca aggccggttt
     2461 tggtgtgcca cggcgtccct catatcattg cgcagcatga aaaaacccgc gcggcggcgg
     2521 gtttatgggg ttagctggcg ggtcttggct tgtcaggcgt cgggctggtc gtggaacttc
     2581 acgcgctgga cgatggtttg gggctgcccg cgatactcgg ccag
//
LOCUS       DATCXU010000377         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_549907,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000377 DATCXU010000000
VERSION     DATCXU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549907"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VIR04_08440"
     CDS             complement(<1..661)
                     /locus_tag="VIR04_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY9460814.1"
                     /translation="MSAVQELKSDIDLSDGRVSIYQPEQSDRIFPSIPTFSNYADERR
                     HIKERLVAACRAFALNGLDYGFAGHLTVRDPENPGLYWTNPMAVHFSQVKVSNLILAD
                     HHGQVVEGRHAINRAGFVLHAAVHEANPDIVAMCHAHTVYGVAFASLGKKLEPITQDA
                     AVFFEDHVVISEEAGQVAVEVKAGNKVANAFKGVKAAIHQNHGLFTVSRHSIEAAAFW
                     FI"
     gene            770..1708
                     /locus_tag="VIR04_08445"
     CDS             770..1708
                     /locus_tag="VIR04_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460815.1"
                     /translation="MRRIPNFALLKAFESAARLESFTLAASELHLTQSAISHQVRALE
                     GYFGRPLFIRRNRRVELTLEGRRLLDSLSRVFDVIEAACDEVALAPRAQVLALHCSPS
                     LAVKWLGPRLRDFLQAHPDITIRLTSGAEPVDLTRMRELDITISYGSAAEQAGVISRP
                     LGVERIAPLCSPALLKTGRPVAEQLGHLTLIDSQLSPVTWPDWFSLNELALPKRPRPS
                     FDRAALAISAAADGMGVALESTRLAEREIGRGQLVEIGPDKFIPIAKEIHFVSCRANE
                     RLTKKIKAFREWLFIQAGINDNAAAPVAINPGHDSR"
     gene            complement(1683..>2141)
                     /locus_tag="VIR04_08450"
     CDS             complement(1683..>2141)
                     /locus_tag="VIR04_08450"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY9460816.1"
                     /translation="VDINAAALRLARINAVLAGAGDLRVVHSDLLRDVPGEFDLIIAN
                     PPYLNDSARRAYRHGGGPLGAGLSLAIVDSAIQRLSAGGVLLLYSGAAIVQGVDTFRT
                     AVEEKLDAAKFHWRYLEIDPDVFGEELLCPAYAGADRIAVVLLTASRAPG"
BASE COUNT          493 a          628 c          586 g          434 t
ORIGIN      
        1 caataaacca gaacgcggcg gcctcgatgc tatgacggct aaccgtgaat aaaccatggt
       61 tctgatggat ggcggccttg acgcctttga aagcattcgc caccttgttg cctgccttga
      121 cctcgacagc aacctggcca gcctcttcgc tgatgacgac gtgatcttca aagaaaacgg
      181 ccgcatcctg cgttatgggc tcgagctttt tacccaatga cgcaaacgcc acgccataca
      241 cggtatgcgc atggcacatc gccacgatat ccggattggc ttcatgcacc gccgcatgca
      301 acacaaaacc ggcgcgattg atcgcatgcc taccctcgac gacttgtcca tggtgatcgg
      361 caaggatcaa gttggatacc ttgacctgcg aaaaatgcac ggccatcgga ttggtccagt
      421 acaaacctgg gttttccgga tcgcgaaccg taagatgccc ggcgaatccg taatcgagcc
      481 cgttcagtgc aaaagcgcga cacgcggcga cgagccgttc cttgatatgc cggcgctcgt
      541 ctgcgtaatt tgaaaacgtg ggaatggaag ggaaaatccg gtccgattgc tccggctgat
      601 aaatggagac ccggccgtcg ctaagatcga tatctgattt taattcctga actgcggaca
      661 tttcgttgct cccaaatggc gatagtgcga tgttggcatt ggaccggcgc gttgacaaac
      721 gatggttctt catggaaaga tgaataccat tcatggataa gagagccaca tgaggcgcat
      781 tcccaatttc gccttgctta aagcattcga gtcggcggcc agactggaaa gcttcacgct
      841 cgccgcgagc gaactgcacc tgacccaatc cgccatcagt caccaggtgc gggcattgga
      901 aggttacttt ggccgcccat tgttcataag gcgtaatcgt cgcgtggaac tgaccctgga
      961 gggccgccgg ctgctcgata gtctttcccg ggtttttgac gttatcgaag ctgcctgtga
     1021 cgaagtcgcg ctggctccgc gggctcaggt gctggcgctg cactgctcgc cgagcctggc
     1081 cgtcaaatgg ttaggccccc ggttgcgcga ttttttacag gctcatccgg acataaccat
     1141 ccgcctgaca tcgggcgccg aaccagtgga tctgacacgc atgcgagagc tggatataac
     1201 gatctcgtac ggctcggcgg cggaacaagc cggggtcata tcccgcccac tgggcgtcga
     1261 gcgcattgcc cctttgtgct caccggcgct actcaaaacc ggcaggccag ttgcggagca
     1321 acttggtcac ctgacattaa tcgattcaca acttagtccg gttacatggc cggactggtt
     1381 ttcgctgaac gaactggcgc tacccaagcg gcccagaccc tctttcgatc gagccgcgct
     1441 tgccatctca gcagcggccg acggtatggg ggtcgcgctt gaaagcacgc gtttggcgga
     1501 acgtgaaatc ggtcgcggcc aactcgttga aatagggccg gataaattta ttccgatagc
     1561 aaaagaaatc cacttcgttt cctgccgggc aaacgaacgc ctgacaaaaa aaataaaggc
     1621 tttccgagag tggcttttta tccaggcggg cataaacgat aatgcggcgg ccccagtggc
     1681 catcaacccg gggcacgact cgcggtgaga agcacgaccg caatgcggtc ggcgccagcg
     1741 tatgccggac aaagtaattc ctcgccaaat acatccggat caatttccag ataacgccaa
     1801 tgaaactttg ccgcatcaag cttttcttcc accgccgtgc gaaaggtgtc cacgccctgg
     1861 acaatagccg cgccgctgta tagcaacaag acgccgccgg cgcttaagcg ttgtatggcc
     1921 gagtcgacaa tcgccaacga caagccggcg cccaatgggc cgccgccatg ccgataggct
     1981 ctgcgcgccg aatcgttcaa atacggcggg ttggcaataa taagatcaaa ctcgccggga
     2041 acatcgcgca acaaatcgct atgcaccacc ctgagatcgc ccgcgcccgc caggactgcg
     2101 ttgatccgtg ccagacgcaa agcggcggcg ttgatgtcaa c
//
LOCUS       DATCXU010000378         5450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_273373,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000378 DATCXU010000000
VERSION     DATCXU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273373"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..808
                     /locus_tag="VIR04_08455"
     CDS             257..808
                     /locus_tag="VIR04_08455"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY9460817.1"
                     /translation="MKFFSLEHSPIAYAADFVAYPAIILGGFVAILAYTPTSRWPVLV
                     AIVVLGLMAWTLIEYGLHRLVLHGLQPFKRWHEEHHNRPHALIGTSTPASMALFAILI
                     FAPIALSTDIWYATAATIGVTSGYLFYVTVHHALHHWRAKNGTWFYDLKRAHALHHRP
                     GFVGYYGVTTLFWDKVFTRHHAS"
     gene            896..2344
                     /locus_tag="VIR04_08460"
     CDS             896..2344
                     /locus_tag="VIR04_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY9460818.1"
                     /translation="MAQDAVATDTAAHAKEWLSGLACSMSREHDYYPTIEGRLPAALQ
                     GTLYRNGPGRFEFGGARKTHLLDGDGMIQAFDIHNGRVRYRNRFVRTEKYLAEERAGR
                     LMLPTWTTRAPGGVLRNIGNRIKSQAGVTTLVKNSRLLAFDEVGLPYGLDPQTLATIG
                     LQQVGPPDVHPDYKAHTKTDPETGNWLLLGVENGARQRLHLVEHAADGTLLRHHRIDS
                     PRQSYIHDWFATERYVLVLLHPLRVSMGRFLSGLSSFTDSMRWEPGKGNLLVVIDRNA
                     AAEPVTIETSACFMWHSLNAFEAGDSIIADFVGYDEPDHFMGEDAAFRAIMYGRQGLQ
                     ECSGTVRRYVVKLRQKQIIEQVLSDENHEFPVLDPRKALHRHRFGYFTTGPPGTVFHN
                     GLARLNVDTGQRDVATLGDCVHLGEPIFVPDREASDEQGWLLSVGLDGSSGRSFLGIF
                     RSERLADGLLAKVLLEHPTPLSFHGWWSGLSV"
     gene            complement(2341..3402)
                     /locus_tag="VIR04_08465"
     CDS             complement(2341..3402)
                     /locus_tag="VIR04_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY9460819.1"
                     /translation="MNGTLQYRSFLLLLILVTLAFGWLLWPFYGAIFWGAILAILFAP
                     LQRRLLVKMRGRRNLAALTTLTTCLLIVIIPLIVIAGSLVKEGSLFFKKIQSGEVNPG
                     AIFERMVNTLPPSIHETLDRFGLSDVFSLQDKLSAGAMQASKFLATQAFSIGQNTFDF
                     LIGLAVMLYLLFFLLRDGAQLARQISRMLPLSETHKQHLFRKFATVTRATVKGNIVMA
                     ATQGALGGIMFWALGIDGALLWGVLMAFLSLLPAVGAAIIWVPVAAYLLVIGAISQGI
                     ILILFGVFVMGLVDNVMRPILVGKDTKLPDYLVLISTLGGLTVFGINGFVIGPLIAAL
                     FLSCWDLFPSAVEMREDDV"
     gene            complement(3399..3833)
                     /locus_tag="VIR04_08470"
     CDS             complement(3399..3833)
                     /locus_tag="VIR04_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY9460820.1"
                     /translation="MEQSPQPPHSPDKLDPALFEAMVEQAGDAIIFIDRKGAVRAWNR
                     GAENIFGYSAAEALNNSLDVIIPERLRQAHWDGFNRAIKSGRTRNGDRILTTRSVHKD
                     GHKIYVDLRFCLIADSSGAIAGALAIGRDCTQRYLAEKALKQ"
     gene            complement(3974..5053)
                     /locus_tag="VIR04_08475"
     CDS             complement(3974..5053)
                     /locus_tag="VIR04_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460821.1"
                     /translation="MIELSVENLHLDYGDNPVLKGVSMQLRRGEVVSLLGPSGSGKTT
                     LLRAVAGLEQPKQGRITINEHVVYDSAAAADVPAEMRGLGLVFQSYALWPHKTVYENI
                     AYPLKLRKAPGAEVRERVQNVLNQLGLGALGERHPHQLSGGQQQRVAIGRALVYNPPV
                     LLLDEPLSNLDAKLREEARAFLRELIMRLGLSALMVTHDQGEAMAISDRILLLNNGKI
                     EQQGTPQEMYGSPTTLFCAEFMGSNNRLAGQVTEIQGERARIEGQGWALWGKAGAGLQ
                     TGQNAHAIIRVEQVRLTDNAEDNSVQMPLLTSMYLGDRWEYLFRGENDDLAQGLALRA
                     YGTSNRMPGNYHLALPAEQVWVFPT"
     gene            complement(5046..>5450)
                     /locus_tag="VIR04_08480"
     CDS             complement(5046..>5450)
                     /locus_tag="VIR04_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460822.1"
                     /translation="LRSTIFSVWLAYSVVWMAYGLRLISSTLLQVAPELEEAARSAGA
                     SRAQVTRQITVPLTKYGLIGSWLLMFLIFEREYSTGVYLLTPGTETIGSMLVSLWASG
                     AIDIVAALSFINIILVVVGLGVALRFGVKLHD"
BASE COUNT         1150 a         1539 c         1546 g         1215 t
ORIGIN      
        1 ttgcggctta aagccagcgc aaggcaggcg ctaaagcggc tttttagtca agcccgctat
       61 tatacacact cgtcacgggc ccgttgccga agcggccggg ctgcccgcag taatcgaaaa
      121 atttggctcg tcatcttaat agcgggtaac tagggatatc cctcgttttc ggacgcaatg
      181 taatgcataa tagtaaaaag tggatcacat gtttcatggc gatatttttg catctttgct
      241 tgcatcggga gcgcatatga aattcttctc gcttgagcac agtcccatag cctatgcggc
      301 ggactttgtt gcgtatccgg cgatcattct cggcggcttt gtggccatat tggcctatac
      361 gcccacgtcg cgctggcccg ttcttgtggc aattgttgtg ctgggcttga tggcatggac
      421 tcttatcgaa tacggcctgc atcggctcgt gttgcatgga ttgcagcctt tcaagcgctg
      481 gcacgaggaa catcacaacc ggccgcatgc attgataggc acgtccacac cggccagcat
      541 ggcgcttttt gctattctga tctttgcccc gatcgctcta tcgaccgata tttggtatgc
      601 gacagcggca accatcggcg tgacatccgg gtatttgttt tacgtgacgg tgcaccatgc
      661 actgcatcat tggcgcgcta aaaatggcac atggttttac gatctgaaac gcgctcatgc
      721 tctgcaccat cgcccggggt tcgttggcta ttacggggta accactctgt tctgggacaa
      781 agtattcacc aggcaccacg cttcataggc tttcggcgaa atcgaaagaa atattgcgcg
      841 gtcggccggt cgtagccagg cttgattgcg ggctttgggt cagcaagggg atgtcatggc
      901 gcaagacgcg gttgcgaccg acacagcagc gcacgctaaa gaatggcttt cgggtttggc
      961 atgctccatg tcgcgagagc atgattatta ccccaccata gaagggcgcc tgcctgcggc
     1021 tttgcaggga accctgtatc gcaatggccc gggtcgcttt gaatttggag gcgctcgcaa
     1081 aacccatctg ctcgacggcg atggaatgat acaggcgttc gatatccaca acggccgcgt
     1141 tcgctaccgc aatcgattcg tccggactga aaagtatctg gctgaagaac gcgctggccg
     1201 cctgatgctt ccgacctgga cgacgcgtgc gccgggcggg gtgctgcgca atatcggcaa
     1261 ccggatcaag tcccaggcgg gcgtgaccac cctggtcaag aatagccgct tgctggcctt
     1321 tgacgaggtc ggcctgccat acggcctgga tccccagact ctggctacga taggtctgca
     1381 gcaagttggc ccaccggatg ttcatcctga ttacaaggct catactaaga ccgatcctga
     1441 aacgggcaat tggcttttgc ttggagtcga aaatggcgcg cggcagcgcc tgcatctggt
     1501 tgagcacgcc gccgatggaa cgttgctcag gcatcatcga attgattcac cccggcagtc
     1561 ctacattcat gattggtttg ccactgagcg ctatgtactg gttctgctgc acccgcttcg
     1621 ggtttccatg ggccggtttt tgagcggcct gagcagcttt accgatagca tgcgctggga
     1681 gccggggaag ggaaatctgc tggtggtgat tgatcgcaac gccgcggctg agcctgtcac
     1741 gatagagact tccgcctgct ttatgtggca tagcctgaat gcgttcgagg caggggactc
     1801 catcattgcc gatttcgtcg gctacgacga acctgatcat tttatgggcg aggacgctgc
     1861 gttcagggcc atcatgtacg gccggcaggg gctgcaggag tgttcgggca cggtgcgccg
     1921 ttatgtcgtc aaattaaggc aaaagcaaat tattgagcag gtgctgagcg acgaaaatca
     1981 tgaattcccc gttctggacc cgcgcaaggc gctgcatcgt catcgtttcg gctatttcac
     2041 caccggacca ccaggaaccg tttttcataa tgggctggcg cgtttaaatg tggatacggg
     2101 tcagcgcgat gtggccactc tgggcgactg cgtccatctt ggagaaccga tttttgtgcc
     2161 ggatcgcgaa gcgtccgacg agcagggctg gctgctttca gttggactgg acggcagttc
     2221 ggggcgctcg ttcctgggaa tttttcgttc ggaacgactg gccgatggcc ttcttgcaaa
     2281 ggtgctgctg gaacatccca cgccgctcag tttccacggt tggtggagcg ggctgtctgt
     2341 ttaaacgtcg tcttcccgca tttcaacggc cgatggaaaa agatcccagc acgataagaa
     2401 tagcgccgcg atcagggggc ctatcacgaa tccattaatg ccaaataccg tcaagccgcc
     2461 caacgttgag atgagaacca ggtagtcggg caactttgtg tctttgccca ctagaatcgg
     2521 gcgcatgacg ttgtcgacaa ggcccattac gaagactccg aacaaaatca ggataatgcc
     2581 ctgtgaaatc gcgccgatga ccagcaggta ggcggccacc ggcacccata ttattgccgc
     2641 acccacggcc ggcagcagcg acaaaaaggc cataagcacg ccccacaaca aggcgccgtc
     2701 aatgcccagc gcccagaaca tgatgccacc cagcgcgccc tgggttgccg ccatcacgat
     2761 attgcccttg acggtcgcgc gcgtcacggt ggcgaatttg cgaaatagat gctgtttgtg
     2821 tgtctcgctt agcgggagca ttcggctgat ttgccgcgcc agttgcgcac cgtcgcgcaa
     2881 cagaaaaaac agcagataca gcatgacggc caggccgatc aggaagtcga aggtgttctg
     2941 accgatgctg aatgcctggg ttgccaggaa tttgctggct tgcatcgcac ccgctgatag
     3001 cttgtcctgc agactgaata cgtcggacag tccaaagcga tccagcgttt catggatgga
     3061 gggcggcagg gtgttgacca tgcgttcgaa aattgcccca gggttgactt cgccgctttg
     3121 aattttttta aagaaaagcg acccttcttt caccagcgag cccgcgatca caatcagcgg
     3181 gatgataaca atcagcaggc aggtcgtcag tgtcgtcagc gctgcaaggt tgcgccgtcc
     3241 ccgcattttg acaagcaatc ggcgctgtag cggcgcaaac aatatcgcca ggattgcgcc
     3301 ccagaatatc gccccgtaaa agggccacaa cagccagcca aaggcgagcg tcaccagaat
     3361 caatagaaga agaaagctgc gatattgcag agtgccgttc attgtttaag ggctttttcc
     3421 gccaggtagc gttgcgtgca atcgcgcccg atggcaagag cgccggcgat tgcgccagac
     3481 gagtcggcga tcagacaaaa acgcaaatca acgtatattt tatgtccgtc cttgtgcacg
     3541 gatcgagtcg tcagaatacg gtcgccattc ctcgtgcgcc ctgatttgat ggcccgatta
     3601 aagccgtccc agtgcgcttg gcgcagcctc tcgggaatga tgacatccag gctgttgttc
     3661 aaagcttccg cagcggagta gccaaagatg ttttccgccc cacgattcca ggcccggacg
     3721 gcgcccttgc gatcaatgaa aataatcgca tcgcctgcct gttcaaccat ggcctcaaac
     3781 aaggccgggt cgagcttgtc aggcgagtgg ggcggttgcg ggctttgttc catcagtctt
     3841 cctttcctgg ttcaaggacc tccggccatc attacgttgg acatgcgcgt ggctggagat
     3901 taggactatt ctacaatttc ccccttgaaa agccttcatc ggcggcgact gtccctgaat
     3961 ccaggaattt cgctcaagtc gggaataccc acacctgctc ggcaggcagc gccaggtggt
     4021 agttgcccgg cattcggttg cttgtgccgt acgcgcgcag ggccaggcct tgtgcaaggt
     4081 cgtcgttttc gccgcgaaac aggtattccc aacggtctcc caaatacatg ctggtcagta
     4141 gtggcatttg cacgctgttg tcttccgcgt tatcggttaa tcgaacctgc tcgacacgaa
     4201 tgatggcgtg ggcgttctgg ccagtttgca gacccgcgcc cgctttgccc cacagggccc
     4261 atccctggcc ttcaatgcgc gcgcgctctc cctggatttc ggtgacctgg cccgccagcc
     4321 gattgttgct gcccatgaat tcggcgcaaa acagagtcgt cggcgaaccg tacatttcct
     4381 ggggcgtgcc ttgctgctct attttgccgt tgttcagcag caaaatacgg tcggaaatag
     4441 ccatggcttc gccttgatcg tgcgtcacca taagcgccga caggcccagc cgcatgatca
     4501 gttcgcgcaa gaacgcacgc gcttcttcgc gcagcttggc gtccagattg gaaagcggct
     4561 cgtccagcag caggacgggc gggttgtaaa ccagagcccg tccaatggcc acccgttgtt
     4621 gctggccgcc cgataattga tgcggatgcc gttcgcccaa cgcgcccaaa cctaattgat
     4681 tgagcacgtt ttgcacacgc tcgcgcactt cggcgcctgg cgctttgcgc agcttgagcg
     4741 gataggcgat gttttcatat accgttttgt gcggccacag tgcgtaggac tggaatacca
     4801 ggcccagacc gcgcatctcg gccggcacat cggcggccgc cgcgctgtcg tacacgacat
     4861 gttcgttgat ggtgatgcgg ccttgtttgg gttgttccag tcccgcaacg gcgcgtagca
     4921 gcgtcgtttt gccgctgccc gatgggccca gcagcgacac gacttcgccc cggcgcaatt
     4981 gcattgatac gcctttaagc accgggttat cgccgtagtc cagatgcaag ttttcaaccg
     5041 atagctcaat catgaagctt gactccgaat cgcagggcga cgcccaggcc gaccaccacg
     5101 agaataatat taataaaaga aagcgccgcc acgatgtcaa tagcgcctga tgcccataac
     5161 gagaccagca ttgagccgat ggtttcggtt ccaggcgtca gcagatacac gcctgttgaa
     5221 tattcgcgct cgaaaatcag aaacatcagc agccaggaac cgatcagtcc gtatttcgtc
     5281 aggggaacgg tgatttgtcg cgtaacctgc gcgcgcgatg cgcctgcgct acgtgctgct
     5341 tcttcgagtt cgggcgccac ctgcaacagg gtgcttgaaa ttaaccgcag cccataggcc
     5401 atccacacca ccgaataggc cagccaaacg ctgaagatgg tggagcgtaa
//
LOCUS       DATCXU010000379         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1124446,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000379 DATCXU010000000
VERSION     DATCXU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124446"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VIR04_08485"
     CDS             complement(<1..383)
                     /locus_tag="VIR04_08485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY9460823.1"
                     /translation="MIGSPRTSLEARLVDAALALQTACSLSQAQTLLTYMAQMQHWNR
                     TYNLTALRETDQILVQHLFDSLAIVAPMCRVLSSRTNAVATIVDVGSGAGLPGVVLAT
                     VQSGWQVHCVDAVEKKMAFVRQMSGV"
     gene            complement(380..>2238)
                     /gene="mnmG"
                     /locus_tag="VIR04_08490"
     CDS             complement(380..>2238)
                     /gene="mnmG"
                     /locus_tag="VIR04_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY9460824.1"
                     /translation="TEAALAAARTGASTLLVTHNMETLGQMSCNPSIGGIGKGHLVKE
                     IDALGGAMALATDQAGIQFRQLNRSKGPAVRATRAQADRSLYRRAIRMILQNQPNLTI
                     FQQPVKDLCLEGDRVAGVITQIGLEFRSRTVVLTAGTFLNGLIHVGLDHYSGGRAGDP
                     PATSLGQRLKELKLPQGRLKTGTPPRVDARSIDFSRTEIQGGDSDPIPVFSYMGSADM
                     HPAQMPCWITHTNARTHDIIRSGLDRSPMYSGVIKGVGPRYCPSIEDKIHRFADKASH
                     QVFLEPEGLSTNEIYPNGVSTSLPFDIQIALLRTLPGLENAIIMRPGYAIEYDYFDPR
                     ELSSSLETRAIKGLFFAGQINGTTGYEEAAAQGLLAGLNAARQVRNLDPWMPKRNQAY
                     LGVLVDDLTTQGVTEPYRMFTSRAEYRLSLREDNADLRLTDEGRRLGLVDDIRWDRFN
                     RKRDAVTGEIERLKSTWVNPKTLSSQTAEALLGKQVEHEYSLFDLLKRPHIAYATLMT
                     TTTSDDRLLAGPGLTDPVAAEEVEIFVKYSGYVARQQEEVDKQAAQEDEPIPADINYD
                     SITSLSIEVRQRLNASRPETVGRASRVPGITPAAISLLLVHIKRLQYGRKAA"
BASE COUNT          481 a          660 c          601 g          496 t
ORIGIN      
        1 acacccgaca tctgacgcac aaaggccatt ttcttttcca cagcgtcaac acaatgaact
       61 tgccagccgg attgcaccgt ggccagcact acgccaggca accccgcgcc tgaacccacg
      121 tcgacaattg ttgcgacggc attcgtgcga ctggagagca ccctgcacat tggcgccaca
      181 atggccaggc tgtcaaacag gtgctgcacc agtatctgat ccgtttcacg cagcgccgtc
      241 aggttgtacg tgcgattcca atgctgcatc tgggccatat aggtgagcaa tgtttgcgct
      301 tgcgacaacg aacacgcggt ctgcaatgcc aaagcggcgt cgaccagccg ggcttcaaga
      361 cttgtccgcg gcgacccgat catgccgcct ttcgtccata ctgcaggcgc ttgatatgca
      421 ccaggagcaa ggaaatagcc gcgggcgtaa tgcccggaac gcgacttgcc cgtcccaccg
      481 tttcaggacg gctggcattg agccgctgac gcacttcgat ggacaagctc gtgatcgagt
      541 cgtaattgat gtcggccggg atgggctcgt cttcttgtgc cgcttgctta tccacttcct
      601 cttgctggcg cgctacatag cccgaatatt taacaaaaat ctcaacctct tccgcggcga
      661 caggatcggt caggccgggg ccggcgagca agcgatcatc gctggttgtc gtcgtcatta
      721 aagtggcata cgcaatatgc ggccgcttca gtaaatcgaa aagggaatat tcatgttcaa
      781 cttgctttcc caacaaggcc tcggccgttt gggatgacaa ggtttttgga ttgacccagg
      841 tcgatttcag gcgctcgatt tcgcccgtta ccgcatcacg ttttcgattg aaacgatccc
      901 agcgtatatc atcgaccagt cccagacgcc ggccttcgtc ggtcagacgc agatcggcgt
      961 tatcttcgcg caagcttaag cgatactccg cccgcgacgt gaacattctg taaggttctg
     1021 ttacaccttg tgttgtcaag tcgtcaacca ggacgcccaa atacgcttga ttgcgttttg
     1081 gcatccatgg gtccaggttc ctgacctgcc gcgctgcgtt caaaccggcc agtaatcctt
     1141 gcgctgctgc ttcttcatac cctgtcgtgc cattgatctg gccggcgaaa aacaacccct
     1201 tgatagcgcg tgtttccagc gatgagctta attccctcgg atcgaaataa tcgtactcga
     1261 tggcatagcc gggccgcatg atgatggcgt tctccagacc gggcaaggtc cgcaggagcg
     1321 cgatttgaat atcaaaaggc aggctggtgg aaacaccatt cggatagatt tcattggtac
     1381 tcagcccctc gggctctaaa aacacttggt ggctggcctt atcggcgaac cgatgaatct
     1441 tgtcttcaat ggacggacaa tagcgcggcc ccacaccctt gatcacaccg ctatacatcg
     1501 gcgatcggtc cagaccagac cgaataatgt cgtgggtgcg cgcgttcgta tgcgtaatcc
     1561 agcaaggcat ttgcgctgga tgcatatcag ccgaccccat gtacgaaaag acgggtatcg
     1621 ggtctgaatc gcccccctga atttcagttc gactaaagtc aatggagcgt gcatccaccc
     1681 ttggcggagt gccggttttc agccggcctt gaggtaactt cagttccttc aggcgctgac
     1741 ccaacgaagt cgccggggga tcgccggcgc ggccgccgga ataatgatca agtccgacgt
     1801 gaatcagccc gttcaaaaaa gtacccgccg tcaggacgac cgttcgactg cgaaattcca
     1861 gaccaatttg agtgatgaca cccgctaccc tgtcgccctc cagacaaagg tccttaacgg
     1921 gctgctgaaa tatcgtcaaa tttggctgat tctgcaggat catgcggatg gctcggcgat
     1981 atagggagcg gtcggcttgg gcgcgggtgg cgcggaccgc cgggcccttc gaccgattca
     2041 attgtcgaaa ctgaattccc gcctgatccg tggcgagggc catcgcgcca cccaacgcgt
     2101 cgatctcttt tacaaggtga ccttttccga ttccccctat agacggattg caggacatct
     2161 gccccagggt ctccatgtta tgagtgacca ataacgtgga agcgccggtc cgggccgccg
     2221 ccagggcggc ttcagtcc
//
LOCUS       DATCXU010000380         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_590026,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000380 DATCXU010000000
VERSION     DATCXU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590026"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VIR04_08495"
     CDS             complement(<1..460)
                     /locus_tag="VIR04_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY9460825.1"
                     /translation="MKITDPAIAALATAKSVLLDPWGLTESDMARALGEIFTHKVDYA
                     DLYFQYTRAESWGLEEGIVKTGSFAIEQGVGVRAISGEKTAFAYSDTLSADALLLSAR
                     AVRSIARQGAGRQKVIGQEVPAAHHLYSAIDPLNTMTAPDKVALLGRLEAM"
     gene            complement(489..1301)
                     /locus_tag="VIR04_08500"
     CDS             complement(489..1301)
                     /locus_tag="VIR04_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY9460826.1"
                     /translation="MLKNNFKVAAIQTVSSTSVSDNLEKAGDLINKAATAGAELIALP
                     EFFCLIGRHNSDKVEIREALGRGPIQDFLQSQAQRHHIWLVGGTLPLVSPDADRIYNS
                     CLVFNPEGQRVGRYDKIHLFGFEKEGESFDESLSIRPGDNAPQTFNAPCGRVGLSVCY
                     DLRFPEFFRALGEVSLMIVPSAFTWTTGAAHWEILLRARAIENQCYVLAPAQGGEHEN
                     GRRTWGHSVLIDPWGDIVDDIAQGPGFIAGDIDQRRLADVRAALPALKHRTM"
     gene            1354..1713
                     /locus_tag="VIR04_08505"
     CDS             1354..1713
                     /locus_tag="VIR04_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEY9460827.1"
                     /translation="MTRDTYSSLKLKIEKEIAKLQRQARTLQFKQRTPVISSIIRSMR
                     EYDITPEEITNAFNKKSIKTAAPKSISAAAPKKRVPAKFRHPETGDTWSGRGKTPRWI
                     SAAEAQGQSRNDFLIAY"
     gene            complement(1749..2399)
                     /locus_tag="VIR04_08510"
     CDS             complement(1749..2399)
                     /locus_tag="VIR04_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY9460828.1"
                     /translation="MLRTLKRSRLSMWRRCAAAAAIALLGGCASTLSARVTTFQKWPA
                     QAQGQTYRIVPSASQANNLEYQTFADTVRAAIGPTGLVEAHAGQSARFDVSFVYDNPA
                     AVAWVQSYGDPYFYNGFGPAWGGYYGGMRRGWSGSVMVMPSTVTVPVSIYKNTLEVTI
                     TDNQNHGAEVYRSTAVSASGGDDLPAVMPYLARAVFEGFPGNNGQTRDITYERQRR"
     gene            2521..>3790
                     /locus_tag="VIR04_08515"
     CDS             2521..>3790
                     /locus_tag="VIR04_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY9460829.1"
                     /translation="MRTDIAPTISRQAYQTYPYRIPTVTLRFELDADQTRVSSCLNIE
                     RATNENQPLVLNGKDLTLESIKLDGQTLNAGQYQLDDECLTLFPSAAAFTLEITNLCH
                     PGKNSSLMGLYVSGESLFTQCEAEGFRRITWFADRPDVMSRYRVALCADKTRYPLLLS
                     NGNLLETRELPGNLHEAVWEDPHPKPCYLFALVAGEFSCREQIVRKRSGREALLQVYS
                     DRGSEDKTAWALDSLARALKWDEQRFGLELDLDRFMVVAARAFNMGAMENKGLNIFNS
                     AYVLADPSTATDAGYRAIESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFREQEFS
                     ADMMAHGLSETEATSARAVKRIDDVSLLRMAQFPEDAGPMAHPIRPESYQEIGNFYTA
                     TVYEKGAEVIRMQHTLLGEAGFRAGMDEYFR"
BASE COUNT          946 a         1071 c          966 g          807 t
ORIGIN      
        1 ccatcgcttc caaccggccc agcaacgcga ctttgtcggg cgccgtcatg gtgttcaaag
       61 gatcgatggc ggaatacaaa tgatgcgccg caggtacttc ttgaccgata accttctggc
      121 gccccgcgcc ttggcgcgca atgctgcgca cagcgcgagc cgacaataac aaggcgtcgg
      181 cggacagtgt gtcggaatac gcaaacgccg ttttctcgcc gctgatggcg cgcacgccca
      241 caccctgctc aattgcgaaa ctgccggttt taacaatgcc ttcctcgagc ccccaacttt
      301 cggcgcgggt atattgaaaa tataaatcgg catagtcgac tttatgggtg aatatttcgc
      361 ccaatgcgcg cgccatgtcg gactcggtca ggccccaggg gtcaagcaag actgatttgg
      421 cggttgccaa tgccgcaatc gcggggtcgg taattttcat aagaggatgc tgtaatgata
      481 aataaaggtt acatcgtgcg atgtttcagg gccggcaacg ccgctcgcac atcggccaga
      541 cgccgctgat caatatcgcc cgcaataaaa ccaggcccct gcgcaatatc gtcaacgata
      601 tcgccccatg gatcgattag aacagaatgc ccccatgtgc gccggccatt ttcgtgctcg
      661 ccgccctgag ctggcgccaa tacgtaacat tgattttcaa tcgcccttgc gcgcagcagt
      721 atttcccaat gcgcggcgcc cgtcgtccag gtgaaggcag aaggcacgat catcaggctg
      781 acctcgccca atgcacggaa aaattcaggg aagcgcaaat catagcaaac tgaaaggccc
      841 acgcggccgc agggagcatt gaacgtttgc ggcgcgttat cgccgggccg aatggagagt
      901 gattcatcaa aagattcgcc ttctttttca aaaccgaaca aatgaatttt atcgtagcgg
      961 ccaacccgct gcccttccgg attaaaaacc aggcacgaat tataaatacg atcagcatcg
     1021 gggctaacca agggtaaagt gcccccgaca agccaaatat gatggcgctg ggcttgagat
     1081 tgtaaaaaat cctgaatggg tccgcgccct aatgcctcgc gaatttccac cttatcagaa
     1141 ttatgccggc ctatcagaca aaagaattcc ggcaaggcaa ttaattccgc gcccgccgtc
     1201 gccgctttat ttatcaggtc gcctgctttt tcgaggttgt cggacacgga cgtggatgat
     1261 acagtctgga ttgctgcgac tttaaaatta tttttaagca tatttataaa taattacgat
     1321 aaaataaact ttaatttttt accggaatga ataatgacgc gcgacacata ttcttcgcta
     1381 aaattaaaaa tcgaaaaaga aatagccaaa cttcagcggc aggcgcgaac ccttcaattc
     1441 aaacaacgaa caccggttat atcgtcgatt atccgttcaa tgcgcgaata cgatatcacc
     1501 cctgaagaaa ttacaaacgc ttttaataaa aaatcaatta aaaccgctgc gccgaaatca
     1561 atatcggccg cagcgccaaa aaagcgtgtc cccgccaaat tccgccaccc cgagacaggc
     1621 gatacatggt cgggtcgcgg caaaaccccg cgctggatat ccgccgcgga agcgcaaggg
     1681 caaagccgca atgatttcct gattgcctat taattagctc gcatcccgca gctgcaagcg
     1741 gactgcgatt aacggcgctg ccgctcgtac gttatatcgc gcgtctggcc gttatttccc
     1801 gggaatcctt caaatacagc gcgggccaga taaggcatta cggccggcaa atcgtcaccg
     1861 ccgctcgcac tgacggccgt ggaccggtac acttcagcac catggttttg attatccgtg
     1921 atcgttacct cgagcgtatt tttatatatg ctgaccggca ctgttaccgt tgaaggcatt
     1981 accatcaccg atccgctcca gccgcgcctc atcccgccat aataaccgcc ccaggcagga
     2041 ccgaaaccat tatagaaata cggatcgccg taactttgca cccacgccac agcggccgga
     2101 ttatcataga caaacgatac gtcgaaccgc gcagactgac cggcatgggc ttcaaccagg
     2161 ccggtaggcc cgatcgccgc gcgaaccgta tcggcaaagg tttgatattc cagattattc
     2221 gcctggctgg cgctgggaac gattcggtaa gtctgtccct gcgcctgcgc gggccacttc
     2281 tgaaacgtgg tcacccgcgc cgacagggtc gaggcgcaac cgcccagcaa tgcgatagct
     2341 gccgcggcgg cgcaacggcg ccacatgctt aaacgggatc tcttcaatgt gcgcaacata
     2401 ggttccctca gtatagtgat taagactgtc cgcaatgcat aaagttgcgt tttcacgacg
     2461 aaaacgaggg tatggcgcaa acactacaat agagtcttaa tgactcttag agcttccatc
     2521 atgcgtaccg atattgcacc gacgatcagc cgtcaggcct atcagaccta cccttatcga
     2581 attcccacag tgacgttgcg tttcgaactg gatgcggatc agacccgggt ttcgtcctgc
     2641 ctgaatattg agcgcgcaac caacgaaaat cagccgctgg ttttaaacgg aaaagacctg
     2701 acgctggaat caatcaagct tgatggacaa acattgaacg ccggccaata tcagttggac
     2761 gatgaatgtc tgaccctgtt tccttccgca gccgcgttca ctcttgaaat taccaatctg
     2821 tgccaccctg gcaaaaattc aagcctgatg gggctttacg tttcaggcga gagcctgttt
     2881 acccaatgtg aagcggaagg cttcagacgc attacctggt tcgctgatcg gcctgacgtc
     2941 atgtcgcgct accgcgtggc gttgtgcgcc gataaaacgc gctacccgct actactgtcc
     3001 aatggcaacc tgctggaaac aagggaactg cccggcaact tgcacgaagc tgtctgggaa
     3061 gacccgcatc ccaaaccctg ctatttgttc gcgctggtcg cgggcgaatt cagttgccgg
     3121 gaacaaattg tccggaaacg cagcggccgc gaggcgctac tgcaagtcta cagtgaccgg
     3181 ggttccgaag ataaaacagc atgggcgctc gattccctcg cgcgcgcgct caagtgggac
     3241 gagcaacgct ttggcctgga actggatctg gatcgtttca tggtggtcgc cgcccgggca
     3301 ttcaatatgg gggccatgga aaacaaggga ctcaatatct tcaactcggc ttatgtgctg
     3361 gccgacccct ctacggccac ggatgcaggc taccgcgcga tcgaatcggt aataggacac
     3421 gagtattttc ataactggac cggcaaccgg gttacgtgcc gcgactggtt ccagctttcg
     3481 ttgaaagaag gcttgactgt ctttcgcgaa caagaattct cggctgacat gatggcgcac
     3541 ggtttgtccg agaccgaagc cacgagcgca cgcgcggtaa aacgcattga cgacgtcagc
     3601 ttgctcagaa tggcgcagtt ccccgaagac gccggcccga tggctcatcc gattcgccct
     3661 gaaagttatc aggaaatcgg caacttctac acggctaccg tctatgagaa aggcgccgag
     3721 gtcatccgca tgcaacacac attgctgggt gaagcgggat ttcgcgcagg aatggacgag
     3781 tatttccggc
//
LOCUS       DATCXU010000381         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_273426,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000381 DATCXU010000000
VERSION     DATCXU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273426"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /locus_tag="VIR04_08520"
     CDS             <1..2170
                     /locus_tag="VIR04_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY9460830.1"
                     /translation="IFISGPGHGAPAVISNCYLEGTYSEIYPEKGQDETGMLNLFRAF
                     SFPGQLGSHCTPEVPGSIHEGGELGYSMSHAFGAAFDNPDLIVSVVVGDGEAETGPLA
                     TSWHSNKFLNPIRDGAVLPILHLNGYKIANPTVLARIPAQELEDLFRGYGWTPHVVQG
                     DDPELMHQRMAATVEDCVQQIRAIQKHARTAGDPDRPRWPMIILRSPKGWTGPKEVDG
                     HKVEDFWRSHQVPVTDVQSNPEHLRIVEQWLRSYQPQDLFDDHGALVAELRDLPPRGD
                     RRMSSNPHANGGKLRKALDLPDFRSYGVALDAPGTKKVSATENLGKFLRDVMRRNMKN
                     FRLFSPDENASNRLQDVYQASLKTWVADIKPEDADGTDIAPDGRVMEMLSEHTVEGWL
                     EGYLLTGRHGLFNTYEAFVHVIDSMYNQHAKWLDKSKLEIDWRSPISSLNILISSLVW
                     RQDHNGFTHQDPGFLDLVSNKSAAVTRIYLPPDANCLLSVTDHCLRSKDYVNVIVSDK
                     QVHPVYLDMDAATVHCTKGIGIWEWASNDAGAEPDVVMASAGDILTQEALAAVAILRD
                     RLPDLKIRFVNIVDLYRLQPDTEHPHGLSDRDFDALFTRDKPVIFNFHGYPWLIHKFT
                     YRRTNHANIHVRGYKEKGNINTPLELAIQNQVDRFSLSIDVIDRVPGLQTRGAHLKEW
                     LKKQIIDSVNYAHAEGTDKPEIRNWVWPLPHGAAPLHQAL"
BASE COUNT          519 a          618 c          654 g          511 t
ORIGIN      
        1 gatctttatt tcggggccgg ggcatggcgc gcccgccgtc atttcgaact gctacctgga
       61 aggaacctat tcagagatct atcccgaaaa aggccaggac gaaaccggca tgcttaatct
      121 ctttcgggcg ttttcgttcc cgggccagct cggcagccac tgtaccccag aggttcccgg
      181 atcgattcac gaaggcgggg aattgggtta cagcatgtcg cacgcattcg gggctgcgtt
      241 tgacaacccg gatttgatcg tcagcgttgt ggtgggagac ggcgaggccg agactgggcc
      301 gctggcgaca tcgtggcatt ccaacaaatt tttgaacccg attcgtgacg gagcggtatt
      361 gccgattctt catctgaacg gttacaagat tgccaatccc accgtgctgg cccgtatccc
      421 ggcgcaagag ctggaggatc tcttcagggg atacggctgg acgccccatg tcgttcaggg
      481 cgatgacccg gaattgatgc atcagcgtat ggccgccacc gtcgaggatt gcgtgcagca
      541 aatccgcgct attcaaaagc atgcgcgaac cgcgggcgac cccgataggc cgcgatggcc
      601 catgatcata cttcgatcgc caaaggggtg gaccgggccc aaagaagtgg atgggcataa
      661 ggtcgaggat ttctggcggt ctcatcaggt gccggtcaca gatgttcagt ccaaccccga
      721 acatttgcgg atcgtcgagc aatggttgcg cagttatcag ccgcaggatc tgtttgacga
      781 tcatggcgct cttgtggcgg agttgcgcga tcttcccccc aggggcgatc ggcggatgag
      841 ttccaacccc catgccaacg gcggcaaact gcgcaaagcg ctcgaccttc cggacttccg
      901 cagttatgga gttgcgcttg atgcgcctgg cacaaagaaa gtatcagcga cggagaatct
      961 gggcaaattt ctgcgcgatg tgatgcggcg caatatgaaa aacttccgtc ttttcagccc
     1021 ggacgagaat gcctcgaatc ggctgcagga cgtttaccag gccagcctga agacatgggt
     1081 cgccgacatc aaacccgagg atgccgacgg cacggacatc gcgcccgatg gccgggtaat
     1141 ggagatgctt agcgaacata cagtcgaagg ctggctcgaa ggttatttgc tgacgggccg
     1201 ccatgggcta ttcaatacct atgaggcgtt tgtccatgtt attgattcca tgtacaacca
     1261 gcatgcgaaa tggctggaca aatccaagct tgaaatcgac tggcgatcac ccatttcgtc
     1321 gctcaatatt ctgatttcat cgctcgtctg gcgccaggac cacaacgggt tcacgcatca
     1381 ggatcccggc tttctggatc tggtcagcaa caagagcgcg gcagttacgc gtatttacct
     1441 tccacctgat gcgaactgtc tgcttagcgt gacagatcac tgtttacgca gtaaagacta
     1501 cgtgaatgtg atcgtatcgg ataagcaagt gcatccggtt tatcttgata tggatgccgc
     1561 aaccgtccac tgcaccaagg gtattggcat ctgggagtgg gccagcaacg atgcgggcgc
     1621 cgagcctgac gtggtcatgg ccagcgcggg cgatatcctg actcaagagg cattggccgc
     1681 ggtcgccatc ctgcgggatc gtctgccgga tttgaaaatt cgttttgtga atatcgtgga
     1741 tttgtatcgg cttcagcccg atacggagca tccgcacggc ctgtccgatc gcgatttcga
     1801 tgcgcttttt accagggaca agccggttat ttttaacttt cacgggtacc cctggttgat
     1861 tcacaaattc acttatcgcc gcacgaatca tgccaatatt catgtgcgcg ggtacaagga
     1921 gaagggcaat atcaacactc cgctggagct tgcaattcaa aatcaggtcg accgcttcag
     1981 tttgtcgatc gatgtcatcg accgcgttcc cggcttgcag acgcgcggcg cccatcttaa
     2041 ggaatggctt aaaaagcaaa tcatcgatag cgtcaactac gcccacgctg aaggcacgga
     2101 caagcctgaa attcgcaatt gggtatggcc gttgccgcac ggggcggcgc cgctgcatca
     2161 ggccctttaa tgatgaacgt atatataatt aaaaatttgc aaaaagaagc tgcaaatgcg
     2221 agctggctgg attccccgga aatcgattta tgcttttgtc acagccgcga cactgacgct
     2281 ggctggctgc gaacagccgc ag
//
LOCUS       DATCXU010000382         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_453231,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000382 DATCXU010000000
VERSION     DATCXU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453231"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VIR04_08525"
     CDS             <1..254
                     /locus_tag="VIR04_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY9460831.1"
                     /translation="AGILYGATSGAITSGLGYAVWYTALGGLSSTQAATAQLTVPAIA
                     AGGGALFLAEPVTWHLVVSSVAILGGAAVVILDKQRHLR"
     gene            complement(262..1053)
                     /locus_tag="VIR04_08530"
     CDS             complement(262..1053)
                     /locus_tag="VIR04_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY9460832.1"
                     /translation="MSETQTKARPDPQFASTLAQGIDILACFKAHESALGNKEFSERT
                     GLSRSTVARLTHTLLELGYLRRAPDSRRYRPGMSLLAVSYPLLASLRIRQLAWPLMAQ
                     LAEHINGAVSLVIRDRRQMVYVETVRMNEELHTYPDIGASLPMLSTAAGKAWLCRASS
                     HDRNVVLNQIRVSEPAYYAQHIESLDKAAGDLEKKGYCGNNAQWRPDAYGFAVPMHKP
                     INSNLFVFNCGVPASDGPFIERESDIAHKLVKLVRGVEEILGLRN"
     gene            complement(1050..1835)
                     /locus_tag="VIR04_08535"
     CDS             complement(1050..1835)
                     /locus_tag="VIR04_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY9460833.1"
                     /translation="MSRAPQHSDPAFATTVANGLAVLECFRVAEPTLSNKDLVERTRL
                     SKATISRLTYTLEMKGLLMYDTQLRRYRLGSALLALSYPLLADMRLRQIARPFMKKLA
                     DFACGSVSLGLYDRLHMVYIETCRGHDAIAFRPDIGARLPVLASAMGRAWLAHANEDT
                     REKVINSVLKADPGQVARHAPAWEQARRDFALNGYCSCLGDWQADVHAVAAPLRRTVD
                     GDSLVFNCGVPVARLKDRWLEDKVAPKLLETISEIEAALERQP"
     gene            1969..3135
                     /locus_tag="VIR04_08540"
     CDS             1969..3135
                     /locus_tag="VIR04_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9460834.1"
                     /translation="MIRDEHGFKLFIESLRLFVRERLIPQEATVAKQNEIPEKLVREM
                     AEQGMFGYSIPEEYGGAGMTTEELVRCAIELSQCSVAFRARVGTNTGIGSEGLVADGT
                     PEQKSRYLPKLARGELTGAFALTEPDAGSDATSLKSTARRHGDHYILNGTKCFITNAP
                     LAGLFTVMARTDPADSGARGISAFLVERGTPGLSTGKEYEKMGQAGSPVSEVHFRDCR
                     VPAANLIGGEEGAGFKTAMKVLNKQRIHLAALCIGPAVRMLDEAMTFVANRRQFGHSL
                     MNFQLIQAMIADCQTDIQAARALVLQTARQRDNAEDVALSASICKYFASEACGRVADR
                     CVQMLGGYGYIADNAIERLYRDVRLFRLYEGTSQIHQLNIARLTLKQAGFDPSR"
BASE COUNT          708 a          938 c          950 g          716 t
ORIGIN      
        1 cggccgggat tctgtatggc gcgacatcag gcgcgattac ttccggcctg ggttatgccg
       61 tctggtacac cgctctgggc ggactgagtt ccacccaggc ggctacggcc caattgaccg
      121 tgccggctat tgcggcgggc ggcggcgcgc tatttctggc agaacctgtc acgtggcatc
      181 tggtcgtatc atctgtggcg atactgggcg gcgccgccgt ggtcattttg gacaaacagc
      241 gccatctcag ataggaaccc cttaatttcg caatcccaat atttcttcaa cgccgcgcac
      301 aagtttgaca agcttgtgcg cgatgtcgga ttcgcgctcg atgaatgggc cgtccgaggc
      361 aggcacgcca cagttgaaaa cgaataaatt tgaatttatg ggtttgtgca tgggcaccgc
      421 gaaaccgtag gcatcgggtc gccattgtgc gttgttgccg cagtacccct ttttttcaag
      481 atcaccggcc gctttgtcca gcgattcgat atgttgtgca taatacgcgg gttcgctgac
      541 ccggatctgg ttcagcacaa cattgcggtc gtgcgaggag gccctgcata accacgcctt
      601 ccccgcggcg gtggacaaca ttggcaaaga ggctccaatg tcagggtaag tgtgcagctc
      661 ttcgttcatg cgtacggtct cgacgtagac catttgcctt cgatcgcgaa taaccaaaga
      721 cacggctccg ttgatgtgct cggcgagttg agccattaaa ggccatgcga gttgccgtat
      781 tcgcaaactg gccagcagcg gataacttac cgccagcagc gacattcctg gtcgataacg
      841 gcgactgtct ggagcgcgcc gcaaataacc gagctccagc aaggtgtggg taaggcgcgc
      901 cacagtgctt cgggacagtc ctgtgcgctc cgaaaattct ttattgccca atgcagattc
      961 atgagccttg aaacaagcca gaatgtctat tccctgcgcc agagtcgatg caaactgggg
     1021 gtctggtctg gctttggtct gggtttcgct catggctgac gctccagcgc cgcttcaatt
     1081 tcgcttattg tctccagcag ctttggggct accttatctt ccagccagcg atctttcagg
     1141 cgcgcaaccg gcaccccgca attgaatacc agcgagtcgc catccacggt cctgcgcagc
     1201 ggcgccgcca ccgcgtgcac gtcggcctgc cagtccccta aacatgaaca gtacccattc
     1261 agggcaaaat cgcgcctggc ttgttcccat gcgggcgcgt gacgggctac ttggcccggg
     1321 tcggccttca ggactgaatt aatgaccttt tctcgggtat cttcattcgc atgcgcgagc
     1381 caggccctgc ccatcgcaga ggccagcact ggcagccgcg cgcctatatc cggtcgaaac
     1441 gcgatggcgt cgtgtccccg gcaggtctcg atataaacca tatggagacg atcatacagg
     1501 cccagcgaaa cagagccaca ggcgaagtca gccagttttt tcatgaacgg gcgggctatt
     1561 tggcgtagcc gcatgtcggc cagcaagggg taactcaacg ctaacaatgc cgagccaagc
     1621 cggtaacgcc gcaattgggt gtcatacatc agcaggccct tcatctcaag cgtgtaggtc
     1681 agacgcgaga tcgtcgcctt ggaaagccgc gtgcgttcca ccagatcttt attgctgagc
     1741 gtgggctcgg ccacgcgaaa acactccagc acggccagcc cattggccac ggttgttgca
     1801 aacgcggggt ccgagtgttg tggtgcgcgt gacatttcga ctggggtttc ccgtttgttg
     1861 tttggtaaat tcaatatatc gaaacaaaga tattgacatt ataaatgagt tggtatgctg
     1921 ggtattgctt tacagcaatt gggcttcact aggaccagag accacttcat gattagggac
     1981 gaacacgggt tcaaactgtt tatcgagtcg ctccggcttt ttgtaaggga acggctcatt
     2041 ccgcaggaag caactgttgc caaacagaat gaaattccag aaaagctggt acgtgagatg
     2101 gccgaacaag gcatgttcgg ctattcgata ccagaggaat acggcggagc cggaatgacc
     2161 acggaagagc tggtgcgctg cgcgatcgag ctatctcaat gttcggtggc tttccgtgca
     2221 agggtgggca ccaacacggg aataggatca gaaggtctgg tcgccgacgg aacgcctgag
     2281 caaaaaagcc gctacttgcc caagctggcg cgcggcgaac tcactggcgc cttcgcgctc
     2341 acggaacccg atgccgggtc cgacgccacc tccttgaagt ccaccgcccg acgccatggc
     2401 gatcattaca tactgaacgg aaccaagtgc tttattacca acgcgccgct ggccggacta
     2461 ttcacagtca tggctcgcac cgacccggca gattcgggtg cgcgcggcat atccgcattt
     2521 ttggtcgagc gcggcacgcc agggctttcg actggcaaag aatacgaaaa aatgggccag
     2581 gccggttcgc cggtgtccga ggttcatttc cgggattgcc gcgtgccggc ggcaaacttg
     2641 atcggtggcg aggagggcgc cggcttcaaa accgccatga aggtgcttaa caaacagcgc
     2701 atccacctcg cggccctttg cattggcccg gccgttcgga tgctcgatga agcgatgacg
     2761 ttcgtcgcca accggcggca gttcggccat tcactgatga atttccaatt gatccaggca
     2821 atgattgcgg actgccagac tgatatccag gcggcgagag ccctggtttt gcagacggcg
     2881 cgacaacggg ataacgccga ggatgtcgcg ttgagcgcat caatttgcaa atacttcgcg
     2941 tcagaagcat gcggacgcgt ggccgaccgt tgcgtacaga tgcttggcgg atatggctat
     3001 atcgccgaca acgcaatcga gcgtctctat cgggacgtcc ggttgttccg gctttatgaa
     3061 gggaccagtc aaatccatca actgaacata gccagactca ccttgaaaca ggcgggcttc
     3121 gacccctctc gttgattaga caggagacaa aacgtgatac gcaaattcct taccttaata
     3181 tttgcagccg cgctgtcgag catggctgtg gccactgcgc aagcggccta ccccgaccat
     3241 gccatccgtt tggttgttcc cttttcgccg ggcggatcgg ccgacatcgt tgggcgactg
     3301 atagccgatg cg
//
LOCUS       DATCXU010000383         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_748130,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000383 DATCXU010000000
VERSION     DATCXU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748130"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VIR04_08545"
     CDS             complement(<1..1110)
                     /locus_tag="VIR04_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY9460835.1"
                     /translation="MSSFTLTDWLQQGLDNSSGEPAYRQLYRLVCRAILDGHLEADTR
                     LPSSRTLASDLGIARNTVIQVYEQLTLEGYVNAATGRGTYVEDLSQDLIDHPSVLRNG
                     GAKRPAASVDRGLSMRGQDLINHVGFSNRQMGAFMPGIPDVAGFPFKAWMSIQNRQWR
                     RDASKLMSYAPAGGYDPLRHAVSEYLGSVRSVNSTARQVIITTGIHQAIDVAMRLLCQ
                     IGDIVWIEEPSYWGVRNLVLSSGLKAIPISVDQEGMCPTADDLARPPKLIVVTPSHQY
                     PLGMVMSLARRRMLLEYARQNGCWIIEDDYDSEFRFGSRPLPSLQGLDDAGLVFYAGS
                     FSKTLFPGLRVGYLVVPDNMAIFFAQAVAELYREGQ"
     gene            1216..2481
                     /gene="gabT"
                     /locus_tag="VIR04_08550"
     CDS             1216..2481
                     /gene="gabT"
                     /locus_tag="VIR04_08550"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY9460836.1"
                     /translation="MNSQNQSWQDRKSAAVPNGIGITCDFYTERALNAEIWDVEGRRY
                     IDFAAGIAVANTGHCHPKIVAAITEQLGRFTHTAFQVSPYSSYVELAEKINQIAPGSH
                     KKKTAFFTTGAEAVENAIKIARAATGRTGIIAFSGAFHGRTYMGLALTGKVAPYKLGF
                     GPFPGEIYHAPYPSALHGVSSQDSLDAISALFKTDIDPKRVAAIILEPVQGEGGFYVA
                     PVEFVQAVRALCDQHGILLIVDEVQTGFGRTGKMFAIEHYGVEPDLMTIAKSLAGGMP
                     LSGVCGRAEIMDAPVPGALGGTYAGNPLALASALAVQDIIASEKLVERANTTGKKLRD
                     TLQAIQTATPEIAEIRGLGAMVAVEFMHADSRKPYPEFAARVQKKALENGLILLTCGV
                     YGNVIRFLFPLTIPDSVLDEGLGILTGVF"
     gene            2510..3613
                     /locus_tag="VIR04_08555"
     CDS             2510..3613
                     /locus_tag="VIR04_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460837.1"
                     /translation="MAHANAEPLVSFKGIQKSYDGVNLVVKNLDLDIYPGEFLSLLGP
                     SGSGKTTCLMMLAGFEFPTGGDIYLERQRLNNVPPHKRNIGMVFQNYALFPHMTVEKN
                     LEYPLRVRKVPAAQRNTRIQDALSMVQMGAFAKRYPAQLSGGQQQRIALARALVFNPK
                     LVLMDEPLGALDKQLREHMQLELKALHKKLGVTFVYVTHDQSEALTMSDRVAVFDQGI
                     IQQISSVSTLYESPGNRFVAGFVGDCNQFEGVVQSRSEGTCVVSLNNGISMTGLDVNQ
                     TPIGAPCVLAIRPERLNIIDPAARPGGNVFKANALDSVYFGDHVRLRCSIPESAHFFV
                     KLPFGDSSSIQSLVYGSEVLLCVSPEHVRVFSP"
     gene            3667..>4702
                     /locus_tag="VIR04_08560"
     CDS             3667..>4702
                     /locus_tag="VIR04_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460838.1"
                     /translation="MKIQTIFCTLSLAATSAVITPAQARDLTVVNFGGANGDAQMVAF
                     VKPLEKLTGEKIIAVSYNGEQAKVKAMVETHNVVWDAVEVETGDLGRGCDEGLYEKLD
                     WSKIGNKADYIPESVRECGVGAFVWSTALAYNGKTLKTPPKSWSDFWDVKKFPGKRGM
                     RKGAHYNLEFALMADGVAPKDVYQVLGTPAGVDRAFKKLDQLKPNIQWWEAGAQPPQM
                     LAAGDVIMSTAYNGRIDAAQREGRPLKVVWNQSIYDLDYWVIPTGSPNKAAAEKYIKF
                     ATSTDPQVAYAQHIAYGPVNKQALSKLSPKNLVDLPNSPENGKNALFSNYEFWADHGE
                     ELEQRFTAWAA"
BASE COUNT         1115 a         1338 c         1275 g          974 t
ORIGIN      
        1 ctgaccctcc cgataaagtt ccgccaccgc ctgagcaaaa aaaatggcca tgttgtctgg
       61 aaccaccaga tagccgactc tcaagccggg aaacagggtt ttgctgaaac tgcctgcata
      121 aaacaccagt ccggcgtcat cgaggccctg caaagaaggc agcggccggc tgccaaaacg
      181 aaattcgctg tcgtagtcat cttcaatgat ccagcatcca ttttggcggg cgtattcaag
      241 caacatgcgg cgccgcgcaa gactcatcac catacccagc ggatactggt gggaaggcgt
      301 gaccacaatc agtttgggcg gcctggccag atcgtcagcg gtcgggcaca tgccctcttg
      361 atcgacactg attggaatgg ccttaaggcc cgaagacagc accagattgc gtacccccca
      421 gtaggaaggt tcctcgatcc agactatatc gccaatctgg cacaacaggc gcatagccac
      481 atcgatcgcc tgatgtatgc cggtggtaat gatcacctgc ctcgccgtgc tatttaccga
      541 acgcacactc cccagatact cggagactgc gtgccgcaag ggatcgtagc cgcccgcggg
      601 cgcatagctc atcaattttg acgcatcgcg gcgccattgc cggttttgta tggacatcca
      661 ggctttgaac ggaaatcccg ccacatcggg aatgcccggc atgaatgcgc ccatttggcg
      721 gttggaaaaa cccacatgat tgatcaggtc ttgcccacgc atggaaagtc cgcgatcgac
      781 ggaggcggcc ggccgcttcg caccgccatt cctcaggacc gagggatgat cgatcaggtc
      841 ctggcttaaa tcttccacat aagtgccacg tccggttgcc gcattgacat agccctccag
      901 tgtcagttgt tcatacacct gaataaccgt gttgcgcgca atccccaggt cggaagccag
      961 ggtgcgcgac gaaggcagac gggtatccgc ctcgagatgg ccatcaagaa tggcgcggca
     1021 aacgagacgg taaagttgcc gataagccgg ttcgccactg ctgttatcca ggccttgctg
     1081 caaccagtca gtgagcgtga atgagctcaa attggctcca taaaaattac actaatggct
     1141 ctggattgta gaccatggcg ccgctatagt agtgaatcat ttgcagcaaa cgaccaccgg
     1201 cgagagcgtc aaaacatgaa tagtcagaat cagtcctggc aggacagaaa atcggccgcc
     1261 gtcccaaacg gcataggcat cacgtgtgat ttttacaccg aacgcgcatt gaatgcggag
     1321 atctgggatg tcgaaggccg ccgatacatc gactttgccg caggcattgc agtggccaat
     1381 acgggccatt gccacccaaa aatcgtggcg gccatcaccg agcaactggg gcgatttacg
     1441 catacggcat ttcaggtttc gccctactcg tcgtatgtcg agcttgccga aaagattaac
     1501 cagattgctc ccggttcgca caaaaagaaa accgcctttt tcaccacggg cgctgaagcg
     1561 gtcgaaaatg ccatcaagat cgctcgcgcg gccactgggc gcaccggcat tatcgcgttt
     1621 tccggggcct ttcacggacg cacctacatg ggtctggccc ttaccgggaa agtagcgccc
     1681 tataagttgg ggttcggccc cttccccggc gagatttatc acgctcccta ccccagtgcg
     1741 ctgcatggag tcagcagcca ggactcactg gatgccatct ccgcgctttt taaaactgac
     1801 atcgatccga agcgggtagc cgccatcatc ctggagccgg tgcaaggcga aggcggcttt
     1861 tacgtcgcgc ccgtggaatt cgttcaggcc gtgcgcgccc tgtgcgacca acatggaatt
     1921 ttgcttatcg tggacgaagt acaaaccggc ttcggccgga ccggaaaaat gttcgccatc
     1981 gaacattacg gcgtggagcc ggatttgatg accatcgcca agagcctggc gggcggcatg
     2041 ccgctatcgg gtgtgtgtgg acgagccgaa atcatggatg cgccggtacc cggcgcactg
     2101 ggaggcactt acgccggcaa ccctctggcg ctggcctctg cgctggccgt gcaagatatt
     2161 atcgcgagtg agaagctcgt ggaacgcgcc aatacaacgg gcaaaaaact gcgtgatacg
     2221 ctgcaagcca tacagacagc aacgccggaa atcgctgaaa tccgcgggct cggggcaatg
     2281 gttgccgtcg aattcatgca tgccgattcc cgcaagccct acccggagtt tgccgctcgc
     2341 gtgcaaaaaa aagctctgga aaatggtctg attctgctaa cgtgtggagt atacggaaac
     2401 gtgatccgct ttttgtttcc acttaccatt cccgattcgg tgctcgatga aggcctgggc
     2461 attcttaccg gtgtttttta actccttata cagcgggtga aaaaatatct tggctcacgc
     2521 taacgcagaa ccgctggtca gcttcaaggg cattcaaaaa tcctacgacg gtgtaaacct
     2581 tgtcgtcaaa aaccttgatc tggatattta cccgggcgag ttcctgtcgt tgctcgggcc
     2641 gtcgggttcc ggcaagacca cctgtctgat gatgctggcc ggtttcgaat ttccgaccgg
     2701 cggcgatatc tatctggaac ggcagcgttt gaacaatgtg cctccgcaca aacgcaacat
     2761 tggcatggtt tttcaaaact atgccttgtt cccccacatg acggtggaaa aaaatctgga
     2821 gtacccgctg cgtgtgcgaa aggtgcccgc cgcgcaacga aacacgcgca ttcaagatgc
     2881 gctttccatg gtgcagatgg gcgcatttgc gaagcgttat ccggcgcaat tgtcgggcgg
     2941 acagcagcaa cgcattgcac tggcgcgcgc actggtgttc aaccccaaac tggtgctcat
     3001 ggacgaacct ttgggggcgc tcgacaagca attgcgtgaa cacatgcaac ttgagcttaa
     3061 ggcactgcat aaaaaattgg gtgtcacttt cgtttatgtg acccatgacc aaagcgaagc
     3121 actgacaatg tccgaccgcg tcgcggtgtt cgatcaggga atcattcaac aaatatcatc
     3181 cgtcagcacg ctttatgaat cacccggcaa ccggtttgtg gccggattcg tcggcgactg
     3241 caaccagttt gaaggagtcg tccagtccag atcggaggga acctgcgtgg tgagcctgaa
     3301 caacggcatc tccatgacag ggctggatgt aaatcaaacc cccatcggcg caccctgcgt
     3361 gctcgccata cggcccgaac gattaaacat catcgaccct gcggcccgcc cgggaggcaa
     3421 tgtgttcaag gccaatgcgc tggattccgt ttatttcggc gatcacgtgc ggttgcggtg
     3481 cagcattccc gaatccgccc atttcttcgt caagttgccg tttggcgaca gcagttcgat
     3541 ccagtctctc gtttacggca gcgaggtact gctttgcgtt tcccccgaac acgtccgggt
     3601 attcagtcct tagttttttt cgcgtcagtt tttcttcgcg tcatgtagac acttatggag
     3661 accatcatga aaattcaaac aatattctgc actttaagtt tggccgcaac gtccgccgtt
     3721 atcactccgg cgcaagcccg cgatctcacc gtcgtcaact tcggaggagc gaacggcgac
     3781 gcgcaaatgg tcgcattcgt caagccgctg gaaaagctga cgggcgaaaa aatcattgcg
     3841 gtgtcctata acggcgagca agccaaggtc aaagccatgg tcgaaactca caacgtcgtc
     3901 tgggatgccg tggaggtcga aaccggtgac ctgggccgcg gctgcgacga aggtctgtac
     3961 gaaaaactcg actggtcaaa aatcggcaac aaagccgact atatacctga gtccgtccgt
     4021 gagtgcggcg tgggcgcttt cgtgtggtct accgctctcg cgtacaacgg caaaaccctg
     4081 aaaacaccgc cgaaaagctg gtcggatttt tgggacgtaa agaaattccc gggcaaacga
     4141 ggcatgcgaa aaggcgcgca ctacaatctg gaattcgcgc tgatggccga tggcgttgct
     4201 cccaaagatg tctaccaggt actaggcacc ccggcgggag tggaccgggc atttaaaaag
     4261 ctggatcagc tcaaaccaaa tatccagtgg tgggaagccg gcgcgcaacc tccgcaaatg
     4321 ctggccgccg gcgacgtgat catgtctacc gcgtataacg gccgaatcga cgcggcgcaa
     4381 cgcgaaggcc ggccacttaa ggtcgtctgg aaccagagca tttacgacct tgactactgg
     4441 gtcatcccca cgggttcgcc aaataaggcg gctgcggaaa aatacatcaa gttcgccaca
     4501 tcaacggacc ctcaagtggc ttacgctcag cacatcgctt acggcccagt caataagcaa
     4561 gccttaagta aacttagccc gaaaaattta gtggatttgc ccaattcgcc cgaaaatggc
     4621 aagaacgcgc tattttccaa ctacgagttc tgggccgacc atggcgaaga gcttgaacaa
     4681 cggttcacgg cctgggccgc cc
//
LOCUS       DATCXU010000384         9240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1136947,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000384 DATCXU010000000
VERSION     DATCXU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136947"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /gene="cydD"
                     /locus_tag="VIR04_08565"
     CDS             complement(<1..1589)
                     /gene="cydD"
                     /locus_tag="VIR04_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEY9460839.1"
                     /translation="MAEAEGGSRFSSSSLTREQSQWLLTLTRLSRVYFAWAVAAPVLS
                     GLLLLAQVYLLARVLGETVAYGIPESLALREIAMIMALVVLRALIGWSGARAASRGAE
                     KIKVFLQQALFERLLMLGPQWTRQRVSGELASALIEQVEILDGFFVRYVPSLIAALFL
                     PLAFAVALLPVDWIVALLVLITAPLIPVFMALVGWGAEAASRRHQLALTRLSGFFADR
                     LRGAFTLKLFGRERAEIETVREASNSLSKKTMAVLRIAFLSSAVLEFFAALGVASVAL
                     YVGLTYLGYLNLRASALTLPLGLFCLLLTPEVYGPLRQFAANYHARAAARAAVGQMAT
                     VFGALPGVEDLMNRPIGPACMPSQVLPSAAGRTPSGWPADCALRVQDLTVHAPGRNAA
                     VLKDVSFNLAKGEKAALMGPSGAGKTTLLETLMGLRAASGGMVSINGRNVLSQSQDGG
                     RGKTILIGQQAFFSPVSIADNLRLANAQATDQALLRALRWSCADGFVAALPLGLETRL
                     GANGYGLSGGQLHRLALARLF"
     gene            complement(1610..1747)
                     /gene="cydX"
                     /locus_tag="VIR04_08570"
     CDS             complement(1610..1747)
                     /gene="cydX"
                     /locus_tag="VIR04_08570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEY9460840.1"
                     /translation="MWYFSWILGLGLACAFSILNAMWFELREGEPHNYRNPHAFDDKP
                     V"
     gene            complement(1773..2927)
                     /gene="cydB"
                     /locus_tag="VIR04_08575"
     CDS             complement(1773..2927)
                     /gene="cydB"
                     /locus_tag="VIR04_08575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY9460841.1"
                     /translation="MEQLIPLDYATLRVVWWAILGFLLVAFAVMDGFDLGVIAVLPLV
                     ARQDLERRIAINVVGPVWEGNQVWLIVLGGVAFAAWPLLYAMAFSGFYLAMMLLLIAL
                     ILRPVGFKYRSKMTTPRWRGTWDGILCLCGVLASVVFGVAVGNVIAGVPFGFDHETIR
                     PVYQGSFLGLFTPFPLLCGVLSLCMLAMHGCVLLFWKTTDAVAQRARTAGRWFAIASA
                     VLFVIGGIWLAYGLDGYAITSAVHGGLESNPLSKTVTMSVGAWMANYARWPAMWIAPV
                     LGVAGALCVVLALGAGKGAAAFFASAASVAGIVLTFGFSTFPFLLPSSLDPDSSLTIW
                     DASSSLLSLWIMLMATTLLLPVVVLYTAWVYRVIRGKITPASLDEAPHAY"
     gene            complement(2940..4517)
                     /locus_tag="VIR04_08580"
     CDS             complement(2940..4517)
                     /locus_tag="VIR04_08580"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY9460842.1"
                     /translation="MFDLDVVNLSRFQFAATVMYHFLFVPLTLGLAFLLAIMESVYVM
                     TGRDIWRRMTVFWGALFGINFALGVATGVVMEFQFGMNWAYYSHYVGDIFGAPLALEG
                     LMAFFLEATFVGLFFFGWDRLPKLGHLAVTWLVAFGANLSAIWILIANGWMQNPVGAV
                     FNPITMRMEMTDFAAVIFNPVAQAKFVHTVSAGYVMGAIFVMAVSAWYLLRGRHLEFA
                     KRSMVVAASFGLASALSVVVLGDESGYLTTEHQKMKIAAIEAMWETEPPPASFNLFAI
                     PDQAQQKNHYSIEIPYVMGILSTRSLTEPLLGVKDLVQHARVRIKNGIVAYDALQKVR
                     ANHDDAAARKVFDANWRDLGYALLLKRFTPDVSNATPQDIDQAALSTVPEVPPLFWTF
                     RIMVALGFYFILFFVAAFWLASTGRLQKYRRFLAVAIWSWPLPWIAIECGWFVAEFGR
                     QPWVIEGVLPTFYAASGLSISDLLISLAIYFVLYTGLAIVGAKVMLHTIRTGPDDKPP
                     AARNPLADSPKPAPAAA"
     gene            4774..5205
                     /locus_tag="VIR04_08585"
     CDS             4774..5205
                     /locus_tag="VIR04_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HEY9460843.1"
                     /translation="MNKLLLPLIAAAFAFGPTYSYAAKTAPQALTQSPPPAPYKAVSS
                     LVKLPDFLPGMGQLFVDPNTLPAGPFLAYDHKGKLVSTIYMIPIANMKPDFNLNDLKT
                     PGVKVNHVDMMYNAGHPGVAEPHIHIVLWNVSPKDEARVAK"
     gene            5202..5735
                     /locus_tag="VIR04_08590"
     CDS             5202..5735
                     /locus_tag="VIR04_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY9460844.1"
                     /translation="MNPARRSFIQTGGGVILAGLAGMASLRVSHAEDVIDIAMMGTPG
                     GAKVWFRPIGLLIQPGQTVRWTNRDPGNSHTSTAYHPTFFKKMQRIPEGAKPWDSGFL
                     LPNESYSVTLTVPGVYDYYCLPHEHAGMVGRIIVTAPEPHGWQTHEGNDQSVPEAARA
                     GFPSIDRIMKEKVVQQL"
     gene            complement(5775..8093)
                     /locus_tag="VIR04_08595"
     CDS             complement(5775..8093)
                     /locus_tag="VIR04_08595"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY9460845.1"
                     /translation="MTPSDPDHSHCDHPDPLKDPVCGMSVTQQSPHHLEHEGKPYYFC
                     SAGCRAKFSAEPHKYLAKEQLDERQNHAPAPGTIYTCPMHPEIRWDHPGNCPKCGMAL
                     EPLVPALDDDNPELADFSRRFWWTLPLTLIVTTLAMLGHRLGWFGMATQSWIELVLSL
                     PVVLWAGWPFFVRGIQSIINRSPNMWTLIGLGTSAAFIYSVAATIAPQAFPDTFSSMG
                     RIAVYFEAAVVIISLTLFGQILELKARSKTSAAIKSLLGLAPKTARRILPNGVEEDVP
                     LAHVHEGDRLRVRPGEKVPVDGEVEEGASSVDESMLTGEPLPVSKRPGDKVIGATLNT
                     SGALVIRAQKVGSATVLSQIVQLVAQAQRSRAPMQRMADHVAGYFVVAVVAIALITFF
                     VWGFWGPEPSWVYGLINAVAVLIIACPCALGLATPMSIMVATGRGATQGVLFRDAAAI
                     ENFRKVDTLIVDKTGTLTEGRPVFEQAMPVQGQTADEVLRLAASLDQGSEHPLADAIV
                     GAARERGLMLAAADEFESGAGIGVRGKVEGKRLLLGNTALMLQEGIDVAELSEKAESR
                     RVEGASVMYLAAEGRLAGLLVVSDPVKTSAKETVDELKAAGIRIIMATGDGMSTANAV
                     ARRLGIDEVHGEVKPADKLHLVAKLQAEGRVVAMAGDGINDAPALAKADVGIAMGTGT
                     DVAMSSAQVTLVKGDLRGILAARHLSEATIRNMKQNLWFAFVYNSLGVPLAAGVLYPF
                     AGLLLSPMFAALAMSLSSVSVVANALRLRSAT"
     gene            8198..>9240
                     /gene="chrA"
                     /locus_tag="VIR04_08600"
     CDS             8198..>9240
                     /gene="chrA"
                     /locus_tag="VIR04_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY9460846.1"
                     /translation="MPSNESEVNEIHHDRAWTVFLIFLRLGLTSFGGPVAHLGYFRTE
                     FVLRRRWLGERRYADLVALCQFLPGPASSQVGMAIGLSRAGFKGALAAWAGFTLPSAI
                     LLTLFGLGLATWGAAFPSGALHGLKVVAVAVVAQAVWGMGRHLCTDAPRISIAVASAC
                     AALLWPNAAGQVGVIAVAAVTGLIMFKPSHEPDHAPFSMPVGRRVGLALLGLFFILLL
                     GLPIAAQAWPAQTLAIISAFYRAGSLVFGGGHVVLPLLQSAVVPGGWTTNETFLAGYG
                     AAQAVPGPLFTFAAFLGASMNFPPNGWVGAAICLAAIFAPSFLLVAGALPFWEQIRHG
                     VRTQAALAGVNAAV"
BASE COUNT         2148 a         2932 c         2445 g         1715 t
ORIGIN      
        1 aaaaacaatc gcgccagcgc cagacggtgc aattgaccgc ccgacaaccc atacccgttg
       61 gcgccgaggc gtgtctccaa ccccagcggc agcgcggcca caaatccatc ggcgcaggac
      121 cacctcaacg cccgcaataa agcctgatct gtggcctgcg cattggccaa tcgcaaatta
      181 tcagcaatcg acacaggcga aaaaaaggct tgctgaccga ttaaaatcgt cttcccgcga
      241 cccccatcct gcgattgcga caagacgttg cgaccgttaa tgctgaccat tccgccactt
      301 gcggcacgca agcccatcaa ggtttcgagc aaagtcgttt taccggcgcc gctggggccc
      361 atcaaggccg ccttctcacc cttggcaagg ttgaaagaaa catcttttag caccgctgca
      421 ttgcgaccgg gagcatgcac agtcaagtct tgcacccgta acgcacaatc tgcgggccac
      481 ccgcttggcg ttcgccctgc tgcgctgggc aacacctgtg acggcatgca cgccgggcca
      541 atcgggcgat tcatcaaatc ttccacaccg ggcaaggcgc caaacaccgt tgccatttgc
      601 ccgacagccg ctcgcgccgc ggcgcgagca tgataattgg ccgcaaactg acgcagcggg
      661 ccatagacct cgggcgtcaa cagcaaacag aacaagccca gtggcaacgt taacgccgac
      721 gcacgcaaat tcaggtagcc caggtaggtc agacccacgt acaaggcaac gctggccacc
      781 cccagcgccg cgaaaaattc cagaacagcc gaagacaaaa atgcaatccg caacaccgcc
      841 attgtttttt tgcttaagct attgcttgcc tcacgcaccg tttcgatctc ggcgcgctcg
      901 cgcccaaaca gcttcaaggt gaaagcgccg cgcaagcggt cggcaaaaaa gccggataag
      961 cgcgtcagcg ccaattgatg ccggcggctg gcggcttcgg cgccccaccc cacaagcgcc
     1021 atgaacaccg gaataagcgg cgccgtgatc aacacaagca gcgcgactat ccagtccacc
     1081 ggcaacaacg cgacggcaaa cgccaaaggt aaaaatagcg ccgcaataag cgaaggcacg
     1141 tatcgaacga aaaatccatc caggatttcg acttgctcga tcaaggcgct ggccagttcg
     1201 cctgaaacgc gttgccgcgt ccactgtggc ccaagcatca acaggcgctc aaacaaagcc
     1261 tgctgaagaa acaccttgat tttctcggcg cctcggctgg cagcgcgtgc acccgaccac
     1321 ccgatcaaag cccgcagaac gacaagagcc ataatcatcg cgatttcgcg caacgccagg
     1381 ctctccggta ttccgtaggc aaccgtctca cccagaaccc gcgccagcaa gtacacctgc
     1441 gccagcaaca agagacccga taaaaccgga gccgcaacgg cccatgcgaa atagacgcgc
     1501 gacaaacgcg tcagcgtcaa tagccattgc gactgttcgc gcgtgagcga tgaggatgag
     1561 aaccgggagc cgccttcagc ttcggccaca aaacccccag gcgcgagcgc tataccggct
     1621 tgtcatcaaa tgcgtgcgga tttcgataat tgtgcggctc cccttcgcgc agttcgaacc
     1681 acatcgcgtt aagaatcgaa aatgcacatg ccagccccaa gcccaaaatc cacgaaaaat
     1741 accacatatc gtcacctatc tttgatgcac actcagtacg cgtgcggcgc ttcgtccagt
     1801 gatgccggcg ttatctttcc gcgtatgacc cgatacaccc acgcggtgta caacacgacg
     1861 acaggcagca acagcgtcgt tgccatcagc atgatccaca atgacaataa gcttgaggac
     1921 gcatcccaga tcgtcaggct ggaatcgggg tctagcgacg aaggcagcaa aaacgggaaa
     1981 gtcgaaaatc caaatgtcag tacgatgccg gcgacacttg cggccgatgc gaaaaacgcc
     2041 gctgcccctt tgccggcgcc aagcgccaac accacacata gcgcccccgc cacacccagg
     2101 actggggcta tccacatcgc cggccagcgc gcgtaattgg ccatccaggc acccacgctc
     2161 atcgtcaccg ttttgctcaa ggggttggac tcaagtccgc catggaccgc gctcgtaatc
     2221 gcgtagccat ccagcccata agcaagccat atgccgccaa tcacgaacaa cacggcggaa
     2281 gctattgcga accatcgtcc cgcggtgcgt gcgcgttgcg ccacggcatc cgttgttttc
     2341 caaaacaaca gaacgcaacc atgcattgcc agcatgcaaa ggctcaacac gccgcacagc
     2401 agcggaaagg gcgtaaacaa gcccaaaaaa cttccctggt acacaggccg gatggtttcg
     2461 tgatcgaacc cgaatgggac gccggcaatc acattgccca ccgcgacccc gaacacgaca
     2521 gaagcaagca ccccgcacaa acacaatatg ccatcccatg ttccccgcca ccgcggcgta
     2581 gtcattttgc tgcgatactt gaaaccaacc gggcgcaaga tcagggcgat cagcaacagc
     2641 atcatcgcca gataaaaccc cgagaacgcc atggcgtaca acaagggcca ggccgcgaat
     2701 gcaacccctc ccagcacaat gagccagacc tggttgccct cccagacggg gccgaccaca
     2761 ttgatggcaa tacggcgttc aaggtcctgc ctggccacca gcggcaagac cgcgatcacg
     2821 cccagatcga agccgtccat gaccgcaaaa gccaccagca agaatcccaa tatggcccac
     2881 cagacgaccc gcaacgtggc gtaatcaagc ggaataagct gttccatgct gtcctctctt
     2941 caggccgcgg ccggtgcggg cttcgggctg tcggccaaag ggtttcgcgc tgccggaggc
     3001 ttgtcgtccg ggcccgtcct gatggtgtgc agcatgacct tggcgccgac aatggccaat
     3061 cctgtgtaca agacaaaata aatggcaagg ctgatcagca gatccgatat gcttaagccg
     3121 gacgcggcgt aaaacgtcgg caacacgccc tcgattaccc agggctggcg gccaaactcg
     3181 gcaacaaacc agccacattc aatggcaatc cagggcagcg gccagctcca gatagcgacc
     3241 gcaagaaaac ggcgatattt ttgcagacgc cccgtcgacg ccagccaaaa tgccgccacg
     3301 aagaacaaaa tgaaataaaa ccccagggca accatgattc tgaatgtcca gaatagcggt
     3361 ggcacctccg gcactgtgct cagggccgcc tgatcgatat cctggggtgt cgcattgctg
     3421 acgtcggggg taaaacgctt taacaacaag gcatagccca aatcgcgcca gttggcgtcg
     3481 aacaccttgc gcgccgccgc gtcgtcgtga ttggcgcgca ccttctgcaa ggcatcgtac
     3541 gccacgatgc cattcttaat gcgaaccctg gcatgctgca ccaggtcttt cacgcccagc
     3601 aagggttcag tcaacgaacg cgtgctgagt attcccatga catacggtat ttcgatggaa
     3661 taatggtttt tttgttgcgc ctgatcggga atggcaaata aattaaaact ggccggcgga
     3721 ggctccgttt cccacatcgc ctcgatcgcc gcaatcttca ttttctgatg ctctgtcgtc
     3781 aagtagccgc tttcgtcgcc cagcaccacc accgaaagcg ccgaagccag gccgaaactt
     3841 gccgcaacca ccatggatcg tttggcaaat tcaagatggc gtccgcgcag caaataccag
     3901 gcgctgaccg ccatgacaaa tatggccccc atgacataac cagcgctaac cgtatgcaca
     3961 aacttggcct gagcaaccgg attgaaaatg acggcggcaa agtcagtcat ctccatgcgc
     4021 atcgtgattg gattaaacac cgcgccgaca gggttctgca tccagccatt ggcgatcaga
     4081 atccatatgg cggacaggtt cgccccaaag gcaaccagcc acgtcaccgc caaatgaccc
     4141 aatttgggca aacgatccca gccgaagaaa aacaggccca cgaaagtcgc ctcaaggaaa
     4201 aatgccatca acccttcaag ggcgagcggc gcaccaaaaa tatcccccac atagtggctg
     4261 tagtatgccc agttcattcc aaactggaac tccatcacca cgccggtggc cacgcccagc
     4321 gcaaaattga ttccaaacag cgcgccccaa aatacggtca tgcggcgcca gatgtcgcgc
     4381 ccggtcatga cgtaaacact ttccataatc gcaagcagaa acgccaaacc caaagtcagc
     4441 gggacaaaca gaaagtgata catcactgtc gcggcaaact gaaagcgcga aagattgacg
     4501 acgtccagat caaacatggt tatgctcccg cgctaatagg cgaaatcccc cacacatgtc
     4561 aggcattcaa atattcccgc ctatcgcccc cgccggcaag cacggatacg aaacaaagtt
     4621 tatccagccg gcgggcaacg gcctgcaatt aacgtaaaac tgtgcaaatg cattctgctg
     4681 aaaataatgg gaacgccccg gcaatgaccg gcatctaatg cgtcgacaca tggccgatat
     4741 ggctctgttc attattatta cggagatcct cgtatgaata aactacttct gccccttatt
     4801 gcggcagcgt ttgcatttgg ccccacctac agctacgcgg ccaaaaccgc gccgcaagct
     4861 ttgacccaat cgcctccgcc cgcgccctac aaggcggtta gctcgctcgt caaactgccc
     4921 gacttccttc ctggaatggg acaacttttt gtcgacccca acacgctgcc tgcgggccca
     4981 ttcctggcgt atgaccacaa gggcaaactg gtcagcacga tctacatgat cccaattgcg
     5041 aacatgaagc ccgatttcaa ccttaacgat ctaaagaccc caggcgtaaa ggtcaatcac
     5101 gtcgacatga tgtataacgc cggtcaccct ggcgtcgcgg aaccgcacat tcatatcgtg
     5161 ctctggaacg tgtcgcctaa agatgaagcg cgcgtagcca aatgaatccc gcacgccgca
     5221 gtttcataca gactggcggc ggcgtgattc tggcggggct agccggtatg gctagcctgc
     5281 gcgtcagcca cgccgaagac gtcatcgaca tcgcaatgat gggaacgcct ggcggagcga
     5341 aggtatggtt tcggcccatc ggtctgctga ttcaacccgg tcagacagtg cgctggacca
     5401 atcgcgatcc ggggaattca catacgtcga ctgcctatca ccccactttt tttaaaaaga
     5461 tgcagcgtat tcctgaaggg gccaagccct gggactcggg ctttttattg cccaacgaat
     5521 cctactcggt caccttaaca gttccgggcg tctatgatta ttactgtctg ccccacgaac
     5581 acgcaggcat ggtgggacgc ataatcgtca ctgcgcctga accgcacggt tggcaaacgc
     5641 acgagggaaa cgaccaaagc gtacccgaag cagcacgtgc agggttcccg tccatcgacc
     5701 gcatcatgaa agaaaaggtc gtccagcaac tctagttcgc caccataact attacgctgg
     5761 agccgatgcg gttttcaggt ggcactacgc aagcgcagcg cattcgccac caccgaaacc
     5821 gaactcaagc tcattgcaag cgcggcaaac atgggcgaaa gcagcaggcc cgcaaacggg
     5881 tacaacacgc cggccgcaag tggcacgccc agcgagttgt acacgaaggc aaaccacaag
     5941 ttttgcttca tgttgcgaat agtggcttcg gacagatggc gtgcagccaa aatgcctcgc
     6001 aaatcgcctt tgaccagcgt gacctgagcg ctgctcatgg ccacatcagt gccggtgccc
     6061 atggcaatgc cgacatccgc cttggctaaa gcgggtgcat cgttgattcc atcgcccgcc
     6121 atggccacca cacgtccttc ggcctgaagt ttggcgacaa gatggagctt gtcagccggt
     6181 ttgacttcgc catgcacctc gtcgataccc aggcgccggg ccacagcgtt ggcagtggac
     6241 atcccatcgc cggtggccat aatgattcga attcctgcag ctttcaactc gtctacggtt
     6301 tctttcgcgc tggtcttgac ggggtcggac accacaagca atccagccaa ccggccctct
     6361 gcggccagat acataacgct ggcgccctcg acgcggcggc tttcggcttt ttcgcttaat
     6421 tccgccacgt caatgccttc ctgcagcatc agcgccgtat ttcccaataa caggcgtttg
     6481 ccttcgacct tgccacgaac cccgataccc gccccggatt cgaattcgtc ggccgcggcc
     6541 agcatcaaac cccgctcgcg cgcggcgccc acgatggcat cggccagggg gtgttcgctt
     6601 ccctgatcca ggcttgcggc caaacgcaac acttcatcgg ctgtctgtcc ctgaacaggc
     6661 atcgcctgct caaacaccgg ccggccctcg gtcagagtgc cggtcttgtc cacaatcagg
     6721 gtatcgacct tccggaaatt ttcgatcgcg gcggcgtctc gaaataaaac gccttgtgtc
     6781 gcgccccgcc cggttgccac cataatggac ataggcgtcg ccaaacccag cgcgcagggg
     6841 caggcaatga tcagcacggc caccgcgttg ataagcccgt acacccagct tggttccggc
     6901 ccccaaaaac cccatacaaa gaaagtgatc agcgcaatcg ccacgacggc cacgacaaaa
     6961 taccccgcca cgtggtcggc catgcgctgc atgggcgccc tggaacgctg ggcttgtgcg
     7021 acaagttgca cgatctggga caacaccgtg gccgaaccca ccttctgcgc gcgtatcacc
     7081 agggcgccgg acgtgttcag cgtggccccg atcactttgt cgcccggccg tttgctgact
     7141 ggcaagggct cgccggtcag catggattcg tccacggaac ttgcgccctc ttccacctct
     7201 ccatcaacag gcaccttttc gcccgggcgc acacgcagcc ggtcgccttc gtggacatgg
     7261 gcgagaggca catcttcttc cacgccattg ggcaaaatgc gccgagccgt cttgggcgcc
     7321 aaaccgagca gggatttaat ggccgcggac gtttttgaac gagccttgag ttccagaatt
     7381 tgcccaaaca aggtcagcga aataatgacc accgccgcct cgaagtacac ggcaatgcgc
     7441 cccatggaag aaaacgtgtc tggaaacgcc tgcggtgcaa tggtggccgc cacgctatag
     7501 ataaaagccg ccgacgttcc caatccgata agggtccaca tattgggact gcgattgatg
     7561 atggattgaa tgccgcgcac aaaaaatggc caaccggccc ataggacaac gggcaaagaa
     7621 agcacgagtt caatccagct ttgggtagcc atgccaaacc agcccaaccg gtgccccaac
     7681 attgccagcg tcgtaacgat cagcgtcagc ggcagtgtcc accaaaaccg ccggctgaag
     7741 tcggcaagtt caggattgtc gtcgtcaagc gccggcacaa gcggctccag cgccatgccg
     7801 catttgggac agtttcctgg atgatcccag cggatctcgg gatgcattgg acacgtataa
     7861 atcgtgcccg gtgcgggcgc atggttttgc cgttcgtcca actgttcttt cgcaaggtat
     7921 ttatgcggtt cggcgctaaa tttggcgcgg caaccggcac tgcagaagta gtagggtttc
     7981 ccttcatgtt caaggtgatg aggcgattgt tgcgtcaccg acatgccgca gacggggtct
     8041 ttcaatggat ccgggtggtc acaatgagaa tgatcgggat cggatggcgt catggcttgg
     8101 ctgctttcct catggttgaa ctgaaagctt accccttttt cgaaactcga ttattatcgg
     8161 cgatcataaa accagctcgt attcatgggc gcaatgaatg ccatcaaacg aaagtgaagt
     8221 aaatgaaatt catcacgacc gcgcctggac agtgttcctc atttttttgc gtctgggatt
     8281 gacctctttc ggtggtcccg ttgcgcattt gggctacttc aggaccgaat tcgtcctgcg
     8341 ccggcgctgg cttggcgagc gccgctacgc ggatctggtg gccttgtgtc agtttctgcc
     8401 ggggccggcc agcagtcagg ttggcatggc gatcgggttg tcgcgagcgg ggttcaaagg
     8461 agcgctggcc gcctgggcag gattcacgct gccttcggcg attctcctga cattgttcgg
     8521 gctgggcctt gctacatggg gtgcggcgtt tccctcaggc gcactgcatg gcctgaaggt
     8581 ggtcgccgtc gcggtggttg cgcaagctgt atggggaatg ggccgccacc tgtgcacgga
     8641 cgcgccgcgt atttccattg ccgtcgcctc cgcctgcgcc gccctcttgt ggcccaatgc
     8701 agcagggcaa gtcggcgtga tcgccgtcgc cgcggtgacg ggattgatca tgttcaagcc
     8761 gtcgcatgag ccggaccacg cccccttctc catgcccgtg ggccggcgcg tcgggctcgc
     8821 gctactgggt ctgtttttta ttctgctgct cggcttgcca atcgccgcgc aggcctggcc
     8881 tgcgcagacg ctggcgataa tcagcgcttt ttatcgtgcc gggtcacttg tctttggcgg
     8941 cggccatgtc gtgttgccgc tgctgcaatc tgcggtcgtg cctggcggct ggacgacaaa
     9001 cgaaactttc ctggcgggct acggcgcggc gcaggccgtt cccggcccac tcttcacgtt
     9061 cgccgccttt ctgggcgctt cgatgaactt tccccccaac ggctgggtgg gcgccgcaat
     9121 ttgtttggcc gccatcttcg ccccttcgtt tctgctggtg gccggcgcat tgccattctg
     9181 ggaacagatc cgccacggcg tgcgcaccca ggcggcgctg gcgggcgtga atgccgccgt
//
LOCUS       DATCXU010000385         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_116281,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000385 DATCXU010000000
VERSION     DATCXU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116281"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VIR04_08605"
     CDS             <1..126
                     /locus_tag="VIR04_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY9460847.1"
                     /translation="DRLQINAQNCIHCKTCDIKDPTQNIVWVAPQGGEGPVYNGM"
     gene            330..1505
                     /locus_tag="VIR04_08610"
     CDS             330..1505
                     /locus_tag="VIR04_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY9460848.1"
                     /translation="MKIQSFRRVLGLSALTLAMLAAPGAQAKDTVKIVFIGPLTGGSS
                     SIGLGGRNSADLAVRQHNENPKSKYHYEMTALDDECKPNVAVQVATQAASDKSIIAGV
                     TNYCSSTAISAINVYHRFGLPIIVWGAVLPEITYGQKFKEVHRVNGTMINQEETAAEF
                     MTRQGYKTWVVIHDTTDYGKGHYKYFSESLAKRGGKILAEFGVTPDQQDFTAELTKTK
                     ELNPEVVFVGGVTPFGIRVRSQMDKLGINAQMEGTSGIKSEAYIKGLGDKLAEGSLAF
                     LEGAPLEKLAHGKEFSADYQKQNYAQPPDAYGPFAYAAMGLILDAIEKVGPDRKKVLA
                     ELNKTKDVDTVIGPVTFDDHGQNIVPLVSAYVAQDGKWVVWEDSEYASGKRKLKGHQ"
     gene            1543..>2162
                     /locus_tag="VIR04_08615"
     CDS             1543..>2162
                     /locus_tag="VIR04_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460849.1"
                     /translation="MEIFSQYLVNGLMLGMIYAIVAVGFSLFFGVLDVIQFAHGDILM
                     LGAFAALATYLGLDAIGVDSPWAQLVIMLAISVLVIAVVGALVAKFLVLPLKNAPPLN
                     TLLLTLMLGLVLRELIRLFYPDGSNSQPFPRLLPTETLHLGAFSIRADSAILLAAGFI
                     TILAVHLLITRTKLGLAIRAVAQDSDTARVMGIDFQWIVLLTFGLGS"
BASE COUNT          530 a          590 c          578 g          464 t
ORIGIN      
        1 gaccggcttc aaatcaatgc gcaaaattgc atacattgca agacctgcga tattaaagat
       61 cccacgcaaa acatcgtctg ggtggcgccg caaggcggtg aaggccccgt ctacaacggc
      121 atgtagaccg attctcaggc atgcatgcaa aagccctgcg gcacaatatt caaagaatca
      181 gggcttgaca tacagtcaag ggcggcatac aaccgcgctt agcgacaaat cgcagacaat
      241 aaacagccgg gcaaaaaatg ggcgtcagac ccaacatcag acactggggc gcctgtttga
      301 agcgccgtcg ttcaacaagg agggacacca tgaaaattca gtcgtttcgc cgtgtgcttg
      361 gtctaagtgc actaactctg gccatgctgg ccgcaccagg cgcacaagcc aaagacaccg
      421 tcaaaatcgt ttttatcggt ccactaaccg gaggatcctc ctctatcggt ttgggtggac
      481 gcaattcggc ggatttggcc gtgcgccagc acaacgaaaa tccaaaaagc aaatatcatt
      541 atgaaatgac cgcgctggac gacgaatgca aacccaacgt ggcggtgcag gtggcgactc
      601 aggccgcctc cgacaagtcc attatcgccg gcgtcaccaa ctattgttcc tcgaccgcaa
      661 tcagcgccat caatgtatat caccgcttcg ggcttcccat cattgtctgg ggcgctgtgc
      721 tgcctgaaat cacgtacgga caaaaattca aggaagtgca ccgcgtcaat ggcaccatga
      781 tcaatcagga agaaaccgcc gccgagttca tgaccaggca aggatataaa acttgggtgg
      841 tcatccatga cacaaccgat tatggcaagg ggcattacaa gtatttttct gaatcgctcg
      901 caaaacgcgg cggcaaaatc cttgccgagt tcggcgtaac acccgatcag caggacttca
      961 ccgccgaact gacgaaaacc aaagaactta atcccgaggt cgttttcgtc ggaggcgtga
     1021 ctccatttgg catacgcgtg cgctcgcaaa tggacaagct tggcatcaat gcgcaaatgg
     1081 aaggaacgtc gggcatcaag tccgaggcct atatcaaggg cctgggggac aagctggccg
     1141 aaggctcgct ggccttcctt gaaggcgcgc cactcgaaaa attggctcac ggcaaggagt
     1201 tctccgcaga ttatcaaaag cagaattacg cgcagcctcc cgacgcttat ggccctttcg
     1261 cctatgccgc catggggttg attctggatg ccatcgagaa agtggggccg gaccgcaaaa
     1321 aagtcctggc tgaactgaac aagaccaaag acgtggacac cgtaatcggg ccggtcacgt
     1381 ttgacgatca cggtcaaaac attgtccctc tcgtatctgc ttacgtggct caggatggaa
     1441 aatgggttgt gtgggaagac agcgagtacg cgagcggcaa acgcaaactg aaaggtcatc
     1501 aataaaccgc ccggcgccga ctagaaggaa tcatccatga gcatggaaat ttttagccag
     1561 tatctggtga atggactcat gctcgggatg atctatgcga tcgtcgcggt cggtttcagt
     1621 ctttttttcg gtgtcctgga tgtcattcag ttcgcgcacg gcgatatcct gatgctgggc
     1681 gcgttcgcgg ctttggcgac gtatcttggg ctggacgcca ttggggtcga cagtccttgg
     1741 gcgcagcttg tcataatgct ggcgatatca gtccttgtca tcgcggtagt tggcgccctg
     1801 gttgccaaat ttctggttct gccgctaaaa aacgccccgc ctttgaacac actgcttctg
     1861 actctgatgc tggggctggt tttacgcgaa ctcatcaggc ttttttaccc ggacgggtcc
     1921 aattcgcaac cctttccacg gctgttgcca accgaaaccc tgcaccttgg ggcgttttcc
     1981 atacgcgccg acagcgccat tttactggcg gccggattca tcacaattct tgcggtgcat
     2041 cttcttatca cacgaaccaa actggggctg gcgattcgcg ccgtggccca ggatagcgat
     2101 acggcgcgcg tcatgggcat cgacttccaa tggatcgtgc tgctgacttt cgggctagga
     2161 tc
//
LOCUS       DATCXU010000386         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_116324,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000386 DATCXU010000000
VERSION     DATCXU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116324"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VIR04_08620"
     CDS             <1..708
                     /locus_tag="VIR04_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEY9460850.1"
                     /translation="RSKHSIISPAWAPDGKRLAYVSFESNKPVVYIQDLATGKRAPLA
                     NYKGNNSAPAWAPNGQQLAIVLSRDSISQIYTINADGSGLRRVMRSPLIDTEPQFTPD
                     GQSIIFTSDRGGSPQIYKVPVGGGDGQRITFNGSYNISPGISPDASNLVYVTRRNGAF
                     RIATQNMGSGAEQLLTTGPDDQSPSFAPNGMQILYSSIVGGRSVLAVVSVDGRVRSTL
                     SALNGKVREPTWGPFTK"
     gene            742..1236
                     /gene="pal"
                     /locus_tag="VIR04_08625"
     CDS             742..1236
                     /gene="pal"
                     /locus_tag="VIR04_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY9460851.1"
                     /translation="MSSRIARSLTIAVFAATLAACSSVPLDKNAGAGAGAESASSGQI
                     MDPFNPQSPLAQQRSVYFEFNSYAVADQYRSLVEMHAKYLTGHTQQKVRIEGNADERG
                     GAEYNLALGQRRSDAVARMMTLLGVNSDQIEAVSFGKERPKATGHTEADYAENRRADI
                     TYQR"
     gene            1311..2000
                     /gene="ybgF"
                     /locus_tag="VIR04_08630"
     CDS             1311..2000
                     /gene="ybgF"
                     /locus_tag="VIR04_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY9460852.1"
                     /translation="MSTHSLPLRTVLFTAIVSLSALYAVPAQAFADDEARRAILELRQ
                     QIKQITDQNSQARLQLADQLETLQSELTGMRGDLEKMRWELDINKRAGQEQTGGNAPK
                     VADSQEQSSFDNAMGLFRSGKYKEAAAGFGQFLGSYPQSQLAPEAKFYRGSSLYASKN
                     FKESVQVLQGMVQASPSDPRAADALLVIAASQIEQNNTAGAKSSLQKIIKDYPQSSAA
                     ETAKSRLKILQ"
     gene            2033..2866
                     /locus_tag="VIR04_08635"
     CDS             2033..2866
                     /locus_tag="VIR04_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9460853.1"
                     /translation="MPSVPDLPQYIAQELDYPLTYTQSGAGQPLLLIHGSLCDYRYWR
                     WQIPAFSNQVHVIAPSLRGYWPNALHAESAAFSIAQHTRDLIDFVRSRGEPMHVLGHS
                     RGAHIALALASAVPEMTRSLILADPGFRLPGDPQSHSFQFDVVEKLKRGDLEGALAQF
                     VDTVHGPGTWRQMVSWFKTMVKDNAYTLLSQIHEVDAPFDSGCLQKIDMPVLLMGGAN
                     SPDRYRRRLDVLESLLTQAHRVTVPLAAHGMNLANPKAFNEQVLQFLGQHTQMREARA
                     T"
     gene            2912..>3399
                     /locus_tag="VIR04_08640"
     CDS             2912..>3399
                     /locus_tag="VIR04_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY9460854.1"
                     /translation="MQYRRLGNSNLKVSALCVGTMMFGDQTELAEARRIVASAHEHGI
                     NFIDTADVYSYGASEKMVGELLKNNRHEWVLASKLGNCMSDAPNESNYSRHWIMRELD
                     NSLKRLSTDFLDILYLHRDFPDSNLEEAVRALGDVIRAGKIRAFGLSNFRGWRIAEII
                     RL"
BASE COUNT          807 a          997 c          888 g          707 t
ORIGIN      
        1 cgttcaaagc actcgatcat ctcccccgcc tgggcgccgg acggcaagcg gctggcctat
       61 gtcagcttcg aatccaacaa accagtcgtc tacatacagg acctggccac agggaaacgc
      121 gccccgttgg ctaattacaa aggcaacaat agcgcgcccg catgggctcc aaacggtcaa
      181 cagctagcaa tcgtgctgtc tcgcgatagc atttcccaga tttacaccat taatgcggac
      241 ggttcgggat tgcgccgggt aatgcgttcg ccgcttatcg ataccgaacc gcaatttact
      301 cccgacggtc agtcgatcat ctttaccagc gaccgcggag gcagccccca aatttataaa
      361 gtgccggtcg gcggtggaga tggtcaacga attacgttta atgggagtta caatatctca
      421 cctggcatct cgccagacgc cagcaaccta gtgtacgtta cacgtagaaa tggcgctttc
      481 cgaattgcaa cacaaaacat gggatccgga gccgagcaat tgcttactac tggacccgat
      541 gatcagtcgc ccagttttgc acccaacgga atgcagattc tttacagctc tatcgtaggt
      601 ggacgtagtg tattagccgt tgtatcagtt gatgggcgcg ttcgttcaac gctatcggct
      661 ctcaatggca aagtgcgcga gcccacctgg ggaccgttta ctaaataatt ttcagtgtga
      721 ctctttttca aaggaactta aatgagctcg cgcatcgcaa gaagtcttac aatcgctgtc
      781 tttgctgcca ccttggcggc atgtagctcc gtaccactcg acaaaaacgc tggggcaggc
      841 gccggcgcgg aatcggccag ttcggggcaa attatggatc ctttcaaccc gcaaagccct
      901 cttgcccagc aacgttcggt ttacttcgaa ttcaatagct atgcggtcgc cgatcaatat
      961 cgcagcctcg ttgaaatgca tgcgaaatac ttgaccggcc atacccagca aaaagtccgt
     1021 atcgaaggca atgccgacga acgcggcggt gctgagtaca atctggcttt aggtcaacgc
     1081 cgttccgacg cggttgcacg catgatgacc ctgctgggtg tcaacagcga ccagatcgaa
     1141 gctgtcagct ttggtaaaga acggcccaag gccactggcc acaccgaagc cgactatgcc
     1201 gaaaatcgcc gcgcggatat cacctatcag cgctaacatt caggttgcct ggtcactgta
     1261 gcgccgtggc ggcaatccgc cgcggcgttt ttatttgaac ggaacaaaaa atgagcactc
     1321 attccctacc cttgcgcacc gtcctgttca ccgcaatcgt ttcgctctcg gctttatatg
     1381 ccgttcccgc gcaagcgttc gccgacgacg aagcccgccg agccattctg gagttgcgtc
     1441 agcaaataaa gcaaataact gatcagaaca gccaggcgcg cctgcagctt gccgaccaac
     1501 tggaaacgct gcaaagcgaa ctcaccggga tgcgtggcga ccttgaaaag atgcgatggg
     1561 aactggatat taataagcgc gccggtcagg aacaaaccgg cggcaatgcc cccaaagtcg
     1621 ccgattcgca agaacaatcc tcgtttgaca acgcaatggg actgtttcgt agcggtaaat
     1681 acaaagaagc cgccgccggt ttcggccaat tcctgggtag ttacccccag agccaattgg
     1741 cccccgaagc caagttctat cggggcagca gcctttacgc cagcaaaaat ttcaaagagt
     1801 cggtgcaggt tctgcaaggg atggtgcaag ccagcccctc agaccctcgc gccgctgatg
     1861 cgctgctggt catagcggcc agccaaatag aacaaaacaa cacggcaggc gcaaagagca
     1921 gcctgcaaaa aattatcaag gactacccgc aaagctccgc cgcggaaacc gcaaaaagcc
     1981 gcctgaaaat tttgcaataa ccatccaaac aggcggctag cgtcgcagcc agatgccatc
     2041 tgttccagat ttaccccagt atattgcaca ggaactggac tatccgctga cctacactca
     2101 atcgggcgcc ggtcagccgc tgttgctgat tcatggctcc ctttgcgatt accgatattg
     2161 gcgttggcag attcccgcat tcagcaatca ggttcacgtt atcgcaccca gcttgcgcgg
     2221 ttactggcct aacgccttgc atgccgagag cgccgctttc agcattgccc agcacacccg
     2281 ggatctgatc gattttgtcc gatcccgcgg cgagccgatg catgtactgg gacattcgcg
     2341 cggcgcgcac atcgcgcttg cgcttgccag cgccgtgcct gagatgacac gctccctgat
     2401 tctggccgac cctggcttca gattgccggg cgacccgcaa tcgcactcgt ttcagttcga
     2461 cgtcgtcgaa aagctgaaac gcggcgatct cgagggtgcg ctggctcagt tcgtcgacac
     2521 cgtccatggt cccggaacct ggcgccagat ggtgagctgg ttcaaaacta tggtgaaaga
     2581 caacgcctat acgctcctgt cgcaaatcca tgaagttgac gcgcctttcg attcaggctg
     2641 cctgcaaaaa attgacatgc ccgttttgct gatgggtggc gcgaacagcc ctgaccgata
     2701 ccgccgccgc ctcgatgttc tggagtcgct gttgactcag gctcaccgcg tcactgtgcc
     2761 acttgccgct catggcatga atctggccaa ccccaaagcc tttaacgaac aggtcttgca
     2821 gtttttgggg caacacacgc aaatgcgcga ggcccgagca acatgacagg gaaacttgtg
     2881 ctatttttac attttcaaag atggagccat catgcaatat cgacgtctgg ggaacagtaa
     2941 tctaaaagtt tcggcgctat gcgtgggtac catgatgttt ggcgatcaga ccgaactggc
     3001 cgaagccagg cgcattgtcg cgtccgcgca cgaacacggc atcaacttca tcgacacagc
     3061 cgacgtctac agctatggcg cctccgagaa gatggtgggc gagcttctga aaaacaaccg
     3121 gcacgaatgg gtgctggcga gcaagctggg caactgcatg agcgacgcgc ccaacgagtc
     3181 gaactattcg cgccactgga taatgcgcga actggataac agcctgaagc gcctgtcgac
     3241 cgacttcctg gatatcctgt atttgcatcg tgatttcccc gacagcaatc tcgaagaagc
     3301 cgtccgcgcc ttgggcgacg tcatccgcgc aggcaaaatc cgggccttcg gcctgtcgaa
     3361 tttcaggggc tggcgcatcg ccgagattat tcgcctgtg
//
LOCUS       DATCXU010000387         8033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_870915,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000387 DATCXU010000000
VERSION     DATCXU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870915"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="cpaB"
                     /locus_tag="VIR04_08645"
     CDS             <1..878
                     /gene="cpaB"
                     /locus_tag="VIR04_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY9460855.1"
                     /translation="LIAAWAARQALQGRILQIESDANVRTVGRVVAAYNLPAGVRLTS
                     EHIAVRQFPATLVSSDSLAPERFAELNDNVLLTPLRAGDLILPVHATRARNDSFSSKL
                     AQGRRAVTMPVDALNSVSGLLEPGDLIDLYVSFEYQRQRITAPLLQGVLVLATGAEHS
                     EDTVSTGAASYSTVTLDTAPEDAIKLVAARQGGTITALLRHPHDNRPTQKAVRGNLAS
                     LLGVGASPLSAANRRAPVIYGNRAERSVPGLRPRPATPELAKGVFDLPYIPELVSTSA
                     LAPASMPQPFTDVAD"
     gene            901..2295
                     /locus_tag="VIR04_08650"
     CDS             901..2295
                     /locus_tag="VIR04_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY9460856.1"
                     /translation="MHRPIVLLLFSLILAASAANARQDILLKLQVGEVKVLSHADIAR
                     VAVGDGHVLNAVTTDEKEVIVFARHEGSSALQIWSTGGERHRYQIDVAPEGARQIQKE
                     LRSVLERIPNTRISVVGDKLIIEGDDLSDGDRERVAALSKRYPQLLDFTGQVGWDRMV
                     LLDVQVVEVPRTQLQELGVRWNTATAGGLNAGLGWDTGSRRFDDRPGEAPVPVPFPAT
                     IAAGYFGVNALLSAQIRAMAQTGTAVVLAQPQLLARSGATAEFLAGGEVPYSTTDAKG
                     QSSTSFKPYGVSLKITPRIERNGAVRSRIEVEVSAVDNSLSVPNGPSLKTRRAATEFN
                     VRSGQTLVLAGFLSREDSRNIDKVPGLGDIPILGALFKSKRFQHDETELAIFVTPIVV
                     APDHPDLMRRVERGQAVLDRAFPDQPVINVPVRQASLLPAPAGADPHAGWDPYQGDGS
                     QWVQQRVNEHGLKE"
     gene            2301..4049
                     /locus_tag="VIR04_08655"
     CDS             2301..4049
                     /locus_tag="VIR04_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY9460857.1"
                     /translation="MIDLDLCFEDGTTQARRFSLPISIGRAPECDLRVKAWRVAKCHA
                     RIEQREEGAFLEDFGSLNGTLVNGRRITRYGPLRLGDQIVIGPCLIRLREAPATAIDD
                     TPAPAPARPLGSSELPQAVKPSGGGESAAQHAPVSTVSLSALLHHRRRLHAALLDALD
                     LRRRDVANMSDAALRAEAKIVLSEIIEHDQELPCTMDRNDLLLDVVNEAIGLGPLEPL
                     LADSAISEIMVNRHDEIFVETAGKLERHGSSFSSEQAVLAVIERIVSPLGRRIDESSP
                     MVDARLRDGSRVNAVISPVSLKGASLTIRKFPQHRPTMDDLLRMGAYDASMREFLRVC
                     VALRKNIIVSGGTGSGKTTLLNILSNCIPAAERVVTIEDAAELRLNHTHLVALEARPA
                     NLEGRGKIEIRDLVRNALRMRPDRIVIGECRGGEAFDMLAAMNTGHEGSLTTLHANSA
                     RDALSRLETMILMAGMDLPLTAVREHIAASIDLIVQQARLSDGRRLITAIVEVTGMES
                     GRIQTQELFRYQPGPPPAFAGCGVMPEFFLKSDAQPAQFNIDMFNQHTILHHTAVHFE
                     AGSAGHADDAETSHPW"
     gene            4043..4888
                     /locus_tag="VIR04_08660"
     CDS             4043..4888
                     /locus_tag="VIR04_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY9460858.1"
                     /translation="MVIVFLLCMVVLVAALAWIAQDWANRAYQRYQTAFEKEAKARLS
                     EFFLFFDPAQLWLVNVILCTCLVATIFVVSGAPWLAGCAGAMALVMPQYVIARMRRRR
                     LAHFDEQLPDVLLALAGALRAGSGVQAALRHIVAQAPNPLAQEFGLMLREQRIGVSFE
                     QALTELYVRVPTEGTGLIVSSLKIAVQSGGNLAETLERIAGTLRARLHLLGRVQALTS
                     QGRLQAWVMAGLPFGLALVLDRLDPESMALLWSTPLGWAVIAVILGLELLGILFIRRI
                     VDIQV"
     gene            4903..5778
                     /locus_tag="VIR04_08665"
     CDS             4903..5778
                     /locus_tag="VIR04_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY9460859.1"
                     /translation="MYFWLSCVAAGCSICSLIWVFTRPVVTHGGTSAAYSRRLVLRLV
                     WPWVSVFAALCKPFISWRTRQGLVGLIQVAGLDPVWKAEHIIAMQCLSLLAFGSISGL
                     ALGIGASYGWRHGILVAFLVGGAAAWWPRYRLRRQGAERQRLMLKEFPFLLDMTTLCV
                     EAGLNLQGALQQAAQHGPVGPLRHELRGALADMRAGVPRLVALTELANRTDLLAVRQL
                     ATALGQADQLGMSLGPLLRAQSEQRRAERFLRAERLALEAPVKMLFPMVFCIFPCTFL
                     IIGFPIVIKLMGVEL"
     gene            5775..6131
                     /locus_tag="VIR04_08670"
     CDS             5775..6131
                     /locus_tag="VIR04_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY9460860.1"
                     /translation="MTVHVPPLMLYRATNFWSRMRGLHAYPALSGHRGLWLFPCNAIQ
                     TFGLAAEIDVVFLDICGRILRRVDCLAPNRIAFCLPAKSVVELPRGYCARYPHYLAAI
                     HRSLRWPDPAAYSPVG"
     gene            complement(6113..6886)
                     /locus_tag="VIR04_08675"
     CDS             complement(6113..6886)
                     /locus_tag="VIR04_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY9460861.1"
                     /translation="MQAAILPIASGWQWILDGIRIFRRQPLAMFFWSLTTGFFITVSY
                     LIPLFGQMALIAATPVLTFITLCACRNIAAGRPMLLNMWLDPIRDATLRRRLFGLGFA
                     YLLCCLIGGFAATLPFIDKLMAAIGSDGTINEAALMQAVRGPFITFGLLYVLISALFW
                     HAPALMGWHKIKFTQALFFSMIACWRNKWPFLLYGATWAAVFFAAQFLGDALVGAGVS
                     ASVMQIMLTPVNIMIAAILYCSFYPTYVSVFGANYPTGE"
     gene            complement(6928..7896)
                     /locus_tag="VIR04_08680"
     CDS             complement(6928..7896)
                     /locus_tag="VIR04_08680"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY9460862.1"
                     /translation="MAVFTPVSDSEIRELLTHYTLGELVSLRGITAGIENTNFFLTTT
                     AGEYVLTIFEVLTHDQLPFYIELMHHLAERGIPVPEPQTQTNGQRIASLHGKPCAIVT
                     RLSGGYEPAPSAAHCALAGQTLARTHLAGSTFQIRQCNLRGLPWWRETIPKVLPFLDA
                     SQSELIQSALSEQIRFAATDVYQSLPSGPSHCDLFRDNVLFDGTFEMPKMGGFIDFYF
                     AGRDTWLFDVAVSVNDWCIDQNSGVFEHEKLQAWLKAYASVRPFTQAEQFAWATLLRA
                     AALRFWVSRLYDFYLPRPAQTLKPHDPRHFERILMERYKPVSTTLP"
BASE COUNT         1611 a         2132 c         2471 g         1819 t
ORIGIN      
        1 gcttgatcgc ggcatgggcg gcccggcagg ccctgcaggg tcgaatttta cagattgaat
       61 ctgacgccaa tgtgcgaacg gtcgggcgtg tggtggccgc atataatttg ccagccggcg
      121 tgcggctgac gtccgagcat atcgccgtgc gtcaatttcc cgcgacgctg gtttcaagcg
      181 acagccttgc gcccgagcgt tttgccgaac tcaacgacaa cgtgttgctg accccgttgc
      241 gcgccggaga cctcatttta ccggtgcatg caactcgtgc ccgaaatgat tccttctcca
      301 gtaaattggc tcagggacgg cgagcggtca ccatgcctgt cgacgctctg aattccgttt
      361 cgggtttgct ggagccaggc gatctgattg atttgtatgt gtcgttcgaa tatcagcgcc
      421 agcgtattac tgcgccgtta ctgcaaggcg tgctggtgct tgccacgggc gctgaacatt
      481 cggaagacac cgtttcgacg ggggccgcgt cctattcaac ggttacgctc gataccgcgc
      541 ctgaagacgc cataaagcta gtggcggcgc gccagggcgg cacgatcacg gcgttgctgc
      601 gtcatcctca cgataatcgt cctacgcaaa aagcggtgcg gggcaatttg gcaagcctgc
      661 tgggtgttgg cgcaagcccg ttatccgcgg caaacaggcg ggcgcccgtt atttacggca
      721 atcgcgccga gcgcagcgtg cctggattgc gtccacggcc ggcaacgccc gagctggcga
      781 aaggcgtatt tgatttgcct tatatccccg agttggtcag cacgtcggca cttgcgccgg
      841 cctcgatgcc gcaacctttt accgatgtgg ctgactaagg agaaaacatt gcgaaccgcg
      901 atgcatcggc cgattgtctt gttgttgttc agcctgatcc tcgctgcatc ggcggcaaat
      961 gcgcggcaag acatcttgct caaattgcaa gtgggcgagg tcaaagtgct gtcgcacgcc
     1021 gatatcgcgc gtgtggctgt gggcgatggc catgtgctta atgcagtcac caccgatgaa
     1081 aaagaggtga tagtttttgc gcggcacgag ggttccagcg cgctgcagat ctggtcgact
     1141 ggcggagaac gccatcgcta tcagatcgac gtcgctccgg aaggcgctcg ccagattcaa
     1201 aaagaattgc gcagtgtgct ggaacgtatt ccgaataccc gcatttcagt cgtgggagac
     1261 aagctcatca tcgagggtga tgatttgtca gacggggatc gtgaacgggt tgcggcgtta
     1321 agcaagcgtt atccgcagtt gctcgatttc accggacagg tcgggtggga tcggatggtg
     1381 ctgctggatg tgcaggttgt cgaggtccct agaacgcaat tgcaggaatt gggcgttcga
     1441 tggaatacgg caacagccgg tgggctgaat gccggattag gctgggacac gggatccagg
     1501 cggttcgacg accggccagg cgaagcgcct gttcccgtgc catttccggc gacgatagcg
     1561 gccgggtatt tcggcgttaa tgcgttattg tcagcccaga tccgggcgat ggcgcaaacc
     1621 ggcacagccg tggttctggc tcagcctcaa ctgctggcgc gtagcggggc gacggctgaa
     1681 ttcctggccg gcggcgaggt gccctattcg accactgacg caaaggggca atccagcact
     1741 tcgttcaagc cttacggcgt gtcactgaaa attacgccgc ggatagagcg caacggcgct
     1801 gtccggtcac gtattgaggt cgaagtcagc gctgtcgaca attctttgtc ggtcccgaat
     1861 ggcccgtcac tgaagacacg gcgggcggcg actgaattca atgtgcgttc tggccagacg
     1921 ctggtgctgg cggggttctt gtcgcgcgag gattcgcgca atattgataa agtccccggt
     1981 ttgggcgaca tacccatact gggcgcgtta ttcaagtcga agcgctttca gcatgatgaa
     2041 accgaacttg ccatatttgt gacgcccatt gttgtcgcac ccgatcatcc cgacctgatg
     2101 cgccgggtcg agcgtggaca ggctgtcctt gatcgcgcct ttcccgatca gccggtgata
     2161 aacgtccctg tgcggcaggc gtcgctattg cccgcgccgg ccggcgcgga tccgcacgct
     2221 ggttgggacc cctatcaggg agacggatcg caatgggttc aacagcgtgt caatgagcac
     2281 ggcctaaagg agtaacaaaa atgattgatc tggatctttg ttttgaggat ggcacgacgc
     2341 aggcacggcg tttttccctg cccatcagca taggacgcgc tcccgagtgc gatttgcgag
     2401 tcaaagcctg gcgagtggca aaatgccatg cgcgtattga acagcgcgaa gaaggtgcct
     2461 tcctcgagga ttttgggtcg ttaaacggca cgctggtcaa tggccgtcgc ataactcgat
     2521 acgggccatt gcgcctgggt gaccaaattg tcataggtcc ttgcctgatt cgattgcgcg
     2581 aagcgccggc gactgctatt gacgatacac cggcgcctgc gccggccagg cctttgggtt
     2641 cgtcggaatt gcctcaggct gtgaaaccgt ctggcggtgg tgaatccgca gcacagcatg
     2701 cgccagtgtc aacggtatct ttatcggcgc tgttgcatca tcgccgacgc ttgcatgccg
     2761 cattgctcga tgcgctggat ttacgccgcc gcgatgtcgc caacatgagc gatgccgctt
     2821 tgcgcgccga ggcgaaaatc gtcctttcag aaattattga gcatgaccag gagttgccct
     2881 gcaccatgga ccgcaatgat ttattgctgg atgtggtaaa cgaagcaata gggctcgggc
     2941 cgctggaacc tttgctggcc gactccgcca ttagcgaaat catggtgaac cgccatgatg
     3001 aaatttttgt cgaaacggcc ggaaaactgg agcgtcatgg atccagcttc agtagtgaac
     3061 aggccgtgct cgccgtgatc gagcgtatcg tttcgccgct cggccggcgg attgacgaaa
     3121 gctcgccgat ggttgacgcg cgcttgcgcg atggatcgcg agtcaatgcc gtaatatcgc
     3181 cggtgtcgtt gaagggggca agcctgacta tacggaaatt tcctcaacac cggccgacca
     3241 tggacgactt gcttcgaatg ggcgcttatg acgcaagcat gcgtgaattc ctgcgcgtat
     3301 gcgttgcgct gcgtaaaaat ataatcgtgt ccggcgggac aggttccggt aaaacgacgt
     3361 tgctcaacat attgtcgaat tgcataccgg ccgcggagcg cgttgtcacg atcgaagacg
     3421 ctgcggaatt gcgtctgaat cacacacacc tggtggcgct cgaggccagg ccggccaatc
     3481 tcgagggtcg cggcaaaatc gagatccgcg atttggtgcg caatgcgctg cgcatgcgcc
     3541 cggatcgcat cgtaattggc gagtgccgtg gcggcgaggc cttcgacatg ctggcggcca
     3601 tgaatacggg ccacgagggt tcacttacga cgctgcatgc aaatagcgcg cgcgatgctt
     3661 tgtcgcgact tgaaaccatg atcctgatgg ctggcatgga cttgccattg acggccgtgc
     3721 gcgaacacat cgcggccagt atcgacttga ttgtgcaaca agcgcgttta agcgacggcc
     3781 gccgattgat cacagcgatc gtcgaggtca cgggtatgga aagcggccgc atacagacgc
     3841 aagaattgtt tcgttatcag cccggtcctc cgcccgcatt tgcgggttgt ggcgtcatgc
     3901 cggaattttt tttgaaaagc gacgcacaac cggcgcaatt caatatcgac atgttcaatc
     3961 agcatacgat tttgcaccac acggccgttc actttgaggc aggctctgcc ggtcacgccg
     4021 atgacgcgga aacttcgcac ccatggtaat cgtatttttg ttgtgcatgg ttgtgctcgt
     4081 cgccgcgctg gcgtggatcg cccaggattg ggcgaatcga gcctatcagc gttaccagac
     4141 agcatttgaa aaggaagcca aggcaagact aagcgagttc tttctatttt tcgatccggc
     4201 ccaactctgg ctggtcaatg tgattttatg cacatgtttg gtcgcgacca tttttgtcgt
     4261 ctcaggtgcg ccatggctgg ccgggtgtgc cggcgcaatg gccttggtca tgccgcaata
     4321 cgtaatcgct cgcatgcgcc ggcggcggct ggcgcacttc gatgaacaat tgcccgacgt
     4381 tctgcttgcg ctggcgggcg cactgcgcgc gggctcgggg gtccaagcgg cgctgcgtca
     4441 cattgtcgcc caggcgccca accctcttgc gcaagagttc ggcttaatgc tgcgcgagca
     4501 aagaataggc gtttccttcg agcaggcatt gaccgaactc tatgttcgtg tccccacaga
     4561 aggaacgggg ctcatcgtgt cctcattaaa aatcgcggtg caaagcggtg ggaatctggc
     4621 ggaaacactt gaacgaattg ccggcacttt gcgtgcgcgt ttacatttgc tgggccgtgt
     4681 ccaggcactg acgtcgcaag gccggttgca ggcttgggtg atggcgggtt tgccgtttgg
     4741 tcttgcgttg gtgctggatc ggctcgatcc ggaatctatg gcgctgttgt ggagcacgcc
     4801 gctgggctgg gccgtaattg ccgtgatttt aggcctggaa ctgcttggca tactgtttat
     4861 acggcgtatt gtcgacatac aagtgtaata ggaacggacg tcatgtattt ttggttaagc
     4921 tgcgtcgcgg cggggtgttc gatttgttca ttgatttggg tattcacccg gcctgtcgtg
     4981 acccatggcg gcacaagcgc cgcctattcg cgtcggcttg ttttgcgact ggtctggcca
     5041 tgggtctcgg tgtttgctgc gctgtgcaag ccgttcattt cctggcgcac gcggcagggc
     5101 ctggtcgggt taattcaggt ggccgggctg gatcccgtgt ggaaggccga acatatcatt
     5161 gccatgcagt gcctgtcttt gctggctttt ggctcaatta gcggattggc attgggcata
     5221 ggcgcatcgt acggctggcg acatgggatc ttagtggcat ttcttgtcgg cggcgccgcg
     5281 gcgtggtggc cgcgttatcg gttgcgtcgg caaggcgccg agcgccaacg cttaatgctg
     5341 aaagaatttc cgttcctgct ggatatgacc accttgtgcg tcgaagcagg gctcaatttg
     5401 cagggagcct tgcagcaagc ggctcagcac ggtcctgtcg gccccctgcg tcatgaattg
     5461 cgaggcgcgc tggccgacat gcgggccggc gtgccgcgac tggtggccct gaccgaattg
     5521 gcgaatcgca ctgacctgct ggccgtgcgt caattggcaa cagctttggg ccaagccgac
     5581 cagctcggga tgagtctggg ccctttattg cgggcccaat cggagcagcg tcgcgcggaa
     5641 cggtttcttc gggccgagag acttgccctt gaggcgcctg taaaaatgct gtttcccatg
     5701 gtgttttgca tatttccctg tacatttttg atcataggtt tcccgattgt gatcaagctg
     5761 atgggagtcg agctttgact gttcacgtgc cgccacttat gctgtatcgg gcgactaatt
     5821 tctggtcgcg catgcgcggt ttgcatgcgt atccggcttt gtcggggcat cgcggcctgt
     5881 ggctttttcc gtgcaatgcg attcagacat tcgggcttgc agccgaaatt gacgtggtct
     5941 ttcttgacat ttgcgggcgt attttaaggc gggtcgattg tctggcgcca aatcggatcg
     6001 cgttttgttt accagcgaag tccgtcgttg aactgccgcg cggctattgc gcccgatatc
     6061 cgcactactt agcggccatt catcgatctt tgcgctggcc cgaccccgct gcttattcgc
     6121 cggtcgggta attcgcccca aacacgctga cgtaagtcgg gtaaaagctg cagtacagga
     6181 tggcggcaat catgatgttg acgggggtaa gcatgatttg catgacgctg gcgcttacgc
     6241 ccgcgccaac cagagcgtcg cccaaaaatt gcgcggcaaa gaaaacggcc gcccaggttg
     6301 ccccataaag cagaaacggc catttgttgc gccagcaggc aatcatggaa aaaaacagcg
     6361 cttgcgtgaa ttttattttg tgccagccca tcagtgcggg cgcgtgccag aataaagccg
     6421 agatcagcac atacagtaat ccaaaagtaa tgaacgggcc gcgaaccgct tgcatgagcg
     6481 ctgcttcatt gattgttccg tcgctcccga tggcggccat cagcttgtcg atgaacggca
     6541 gggtggccgc gaatccgccg attaggcagc ataacaaata ggcaaagccc aatccaaaca
     6601 agcgcctccg gagggtcgca tcgcgtatgg gatcgagcca catgttcagc aacattggac
     6661 ggccggcggc aatgttgcgg catgcgcata gcgtgatgaa tgtcagcacg ggcgtggcgg
     6721 caatcagggc catctgaccg aacaggggta ttagatagct tacggttata aaaaaacccg
     6781 ttgtcaggct ccagaagaac atggccaggg gttggcgccg gaagatccgg attccatcta
     6841 gaatccattg ccagccggat gcaatgggaa ggatggcagc ttgcataata attgaggttt
     6901 ccaggaaagt ggaggccagt gtaacggcta cggcagggtg gtggaaacgg gcttgtatcg
     6961 ttccatcaaa atacgctcaa aatgccgcgg atcgtgcggc ttcagtgttt gggcgggccg
     7021 gggcaggtaa aaatcataca ggcgcgacac ccagaagcgc agggcggccg cccgcagcaa
     7081 ggtggcccac gcaaattgtt ccgcctgggt aaaggggcgg acgcttgcgt atgccttcag
     7141 ccatgcttgc aatttctcat gctcgaagac gcccgaattt tgatcgatgc accagtcgtt
     7201 tacgctgacg gcgacatcaa atagccaggt gtcgcgtccg gcgaagtaga aatcgatgaa
     7261 cccgcccatt tttggcattt cgaatgttcc gtcgaacaag acgttgtcgc gaaacagatc
     7321 gcaatgggac gggcccgatg gcaatgactg atagacgtcg gtcgcggcga agcgaatttg
     7381 ctcgcttagg gcgctttgta ttagttcgct ttgcgaggcg tcaagaaatg gaagcacctt
     7441 tggaatcgtt tcccgccacc atggcagacc gcgcaaattg cattgccgga tttggaaggt
     7501 gctgcccgcc aaatgcgtgc gcgccagcgt ttgacccgcc agggcgcaat gcgcagcgct
     7561 gggtgcaggt tcgtacccgc cggataaacg cgtcacaatc gcgcacggct tgccgtgtaa
     7621 actggcaatt cgttgcccgt tggtctgagt ttgcggttcg ggcaccggaa tgccgcgttc
     7681 agccaaatgg tgcatcagct cgatataaaa tggcagttga tcgtgggtca gaacctcgaa
     7741 tatggttagt acatattcgc ccgcggtggt ggtcaaaaag aagttcgtat tctcgatgcc
     7801 ggcggtaatg ccgcgcaatg aaacgagttc gcccagtgtg tagtgggtta gaagttcgcg
     7861 tatctcgctg tcgctgacag gcgtaaaaac ggccatgggg cttgatgctc tatttaaata
     7921 aataaaagcc cgtgaattcg ggcgcttgat tttagtccag gttggaatag tgtccagaaa
     7981 cgttcgcaat gatgcaggcg tgcttcacct taacatgccc ttggcagacg tgg
//
LOCUS       DATCXU010000388         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_275164,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000388 DATCXU010000000
VERSION     DATCXU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275164"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VIR04_08685"
     CDS             complement(<1..730)
                     /locus_tag="VIR04_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEY9460863.1"
                     /translation="MCGLVGIWSRNHYENLREQIESASGVLKHRGPDDSGTWIEGELG
                     LALGHRRLSILDCSAAGHQPMHSHDDRYVTVFNGEIYNHLDLRVQLAADGITPAWRGH
                     SDTETLLACFSAWGIKKTLQATVGMFAIVLWDRQTKVLTLARDRMGEKPLYWGWQNDT
                     LVFTSELKALKSFFSFDGQVDRNALALLLRHGYIAAPYSIYQGIQKLPMGNFIDIPLS
                     SREAAHAARPVAYWKMNESIQTGLD"
     gene            complement(735..1589)
                     /locus_tag="VIR04_08690"
     CDS             complement(735..1589)
                     /locus_tag="VIR04_08690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY9460864.1"
                     /translation="MENPLISAQALAALLLDKSADVLVCDNRFDLVDPAAGARAYAQG
                     HIPGAVHTDLDHALCSARTGKNGRHPLPTREAFAHTMAQLGVNANTRVVAYDANNSTY
                     AARLWWMMRWIGHANVSVLDGGLQAWTDAGNPLSTDKPAARKTGNLQVRPALTKTVDY
                     AQVLDNIGTAARMVVDARSPDRFRGENETLDPVGGHIPDARNRFFKDNLNPSNHFKPA
                     GQLHSEFETLLAGRKAEDVINQCGSGVSACHNLLAMHIAGLDGSALYPGSWSEWCAQP
                     GAPVARGA"
     gene            complement(1748..>2483)
                     /locus_tag="VIR04_08695"
     CDS             complement(1748..>2483)
                     /locus_tag="VIR04_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY9460865.1"
                     /translation="FNDPAKHNAVSLDMWAAIPDALKAFEEDANIRAVVLTGAGEKAF
                     VSGANISQFEALRTGESAVKEYEVVAEKAQRSIHNFSKPIIARIDGYCIGGGMNIALC
                     CDIRLASDTSVFSIPAARLGLGYRFTAIENLVRATGPANALEIFITASKYSASEAKEK
                     GLIQAFCAKAELNSLVDSYLKKINANAPLSMAAGKRMIRLLADRSASIDPGEMKALVL
                     ECFESDDYKEGKLAFSQKRPPEFTGH"
BASE COUNT          494 a          691 c          712 g          586 t
ORIGIN      
        1 tgtccagacc tgtctggatg gattcgttca ttttccagta ggcaaccgga cgggccgcat
       61 gcgcggcttc ccggctggat aacgggatat cgataaagtt gcccatgggc aacttttgaa
      121 tgccctggta aatgctgtag ggcgccgcga tataaccgtg gcgcagcagt agcgcgagcg
      181 cgttgcgatc gacctgcccg tcaaatgaaa aaaaggactt gagcgccttg agttccgatg
      241 taaacacgag cgtgtcgttc tgccagcccc aataaagggg tttctccccc atcctgtcgc
      301 gtgccagcgt cagcactttt gtctgacgat cccatagcac aatggcaaac atgcctaccg
      361 ttgcctgaag tgttttcttt atgccccatg cgctgaagca ggccagcagc gtttcggtgt
      421 cggaatggcc gcgccatgcg ggtgtaattc cgtcggcggc caattgaacg cgcaagtcca
      481 gatggttgta gatttcgcca ttaaagacgg ttacgtagcg atcgtcatgc gaatgcatgg
      541 gctgatggcc cgcagcggag caatcaagaa tcgaaaggcg ccggtggccc aaggccaggc
      601 ccagttcccc ctcgatccac gtgcccgaat catcgggtcc gcggtgcttt aatacgcccg
      661 acgccgactc gatttgttcg cgcagatttt catagtgatt ccgcgaccag attcccacca
      721 atccgcacat attttcaggc tccgcgtgcc accggcgcgc cgggttgcgc acaccattcg
      781 ctccaggagc cagggtaaag agccgagccg tccaggcccg cgatgtgcat ggccagcagg
      841 ttatggcagg cgcttacgcc cgaaccgcat tggttgatga catcttcggc tttccgcccc
      901 gccaacaaag tttcgaattc gctgtgcaat tgaccggcag gtttgaaatg gttggatgga
      961 ttcagattgt ctttgaaaaa acggtttcgc gcatcgggaa tatggccgcc aaccgggtcc
     1021 agagtttcgt tttcgccgcg aaaacggtca gggctgcgag cgtccacgac catgcgggcg
     1081 gccgtgccaa tattatccag aacctgtgcg tagtcgacag ttttggtcaa ggcggggcgg
     1141 acctgaaggt tgccagtctt gcgcgcggct ggcttgtcgg tcgatagtgg gttgccggcg
     1201 tcggtccacg cttgcaggcc gccatcaagg accgacacgt tggcgtgtcc aatccagcgc
     1261 atcatccacc acagtcgtgc cgcataggtc gagttgttgg catcgtacgc caccacgcgg
     1321 gtgtttgcat tgactccaag ttgcgccatg gtgtgcgcaa acgcctcgcg cgtgggcagg
     1381 ggatggcggc cgttctttcc ggttcttgcc gaacacaggg catggtccag atcggtatgc
     1441 acagcgcccg gtatgtggcc ttgcgcgtag gcgcgcgcgc ccgcggcggg gtcgaccaga
     1501 tcgaaccggt tgtcgcagac aaggacgtcg gcggatttat caagcagtaa agcggccagc
     1561 gcttgcgcgc tgatcaaagg attttccatg cgattgcctt tcaattacgt tgtgcaaatc
     1621 tcatgataat ccttgttggc gccgccactg acggcgccag gcccgtcagt caatatggat
     1681 attcagtttc ttgacgattt cggcccattt cgtaagttgg ccattcactt ctcctttgag
     1741 gtgattctta gtggcccgtg aattcgggcg gccgtttttg ggaaaatgca agttttcctt
     1801 ctttgtaatc gtcgctttca aagcattcca ggaccaatgc cttcatttcg ccgggatcaa
     1861 tagacgcgga gcgatccgcc agtagccgga tcattctttt gccggctgcc attgacaagg
     1921 gtgcattggc gttgattttc tttaaatagc tatccaccag gctgttcaat tctgccttcg
     1981 cgcaaaatgc ctgaataaga cctttctctt tggcctcgga agcgctgtat ttcgaggcag
     2041 taatgaaaat ttccaatgcg ttcgcgggtc ctgttgcacg caccaggttt tcgatggccg
     2101 taaagcggta gcccaaaccc agccgcgcag ccgggataga gaaaacggac gtatcgctgg
     2161 ccaatcggat gtcgcagcac agcgcgatgt tcatgccgcc gccaatgcaa tacccgtcga
     2221 ttctggcaat gatgggttta ctgaaattgt gtatcgaccg ttgcgctttt tcggcgacta
     2281 cttcgtattc cttgacggcg gactcgccgg tgcgcaatgc ctcgaactga gatatattgg
     2341 cgccgctgac gaatgccttt tcgcccgcgc ctgtcagcac gacggcgcgg atattggcgt
     2401 cttcttcgaa agccttcagc gcgtcgggta tagcggccca catgtcaagc gagacggcat
     2461 tgtgttttgc cggatcgtta aag
//
LOCUS       DATCXU010000389         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_506742,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000389 DATCXU010000000
VERSION     DATCXU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506742"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VIR04_08700"
     CDS             complement(<1..229)
                     /locus_tag="VIR04_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY9460866.1"
                     /translation="MLDLVIETRCHDLGGFQVGRVLPFRKRRMVGPFIFFDRMGPHDL
                     PPHIPVQTDVRPHPHIGLSTVTYLFDGQMTHR"
     gene            complement(243..797)
                     /locus_tag="VIR04_08705"
     CDS             complement(243..797)
                     /locus_tag="VIR04_08705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY9460867.1"
                     /translation="MTQYQIAVFVGSLRKDSINRKLANALIKLGPPDFSFKQLRIDDM
                     PLYNQDSDGNQAEQVKRLKAEVKAAQGLLFVTAEYNRSIPGVLKNAIDNASRPYGQGA
                     FIGQPAGVLGASIGSIGSAMAQQHLRNVLAYLDSPTMGQPEVFIHITDTFFDAAGNIA
                     NAGSQKFLQGWMDKYVAWVKKHTA"
     gene            complement(815..1942)
                     /locus_tag="VIR04_08710"
     CDS             complement(815..1942)
                     /locus_tag="VIR04_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY9460868.1"
                     /translation="MPLIVAAKFRGLVLMAAIGLIAYASAAFAAEPGITLYGVVDTGI
                     SYTRVQDAEKTPRQRTGLTTGGLSDSLIGMKGQEALAGGWLATFQLESMIDATTGSLA
                     DPSRFFDSNAWVGLANDQFGEIRFGRQRLAGQQFGSLLEIASWKDMPMGATFKASDNY
                     QVDNTVNYLSPSWAGFSFGAGYSFDAIGGQINGRKSPVSSVALKYENGPLLAVATWDK
                     AYLSTPALRDPVRPQAWQLGAAYDFGVAKVSLAWSRQKDGYVGLDGGDPDNLGLGLGA
                     TEFVRGGRLDAYLAGLSIPVGASSSVVAQWSVVKPHWRWQDGENAATGHVATLGYIYN
                     LSSRSRLYAMAGVAKRYSLDDQVVQNQGTTVRYMAGLNHSF"
     gene            1966..2532
                     /locus_tag="VIR04_08715"
     CDS             1966..2532
                     /locus_tag="VIR04_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY9460869.1"
                     /translation="MDGKTENQSNGPVVVCTIGHSNRPIDVFLDLLQTNEVTHVLDVR
                     TIPRSLHNPQFGQDMLPAHLQAAGIGYTHMAGLGGLRHTSPNSPNGAWRNASFRGYAD
                     YMQTEDFAANIEIVIELARKERCVLMCAEAVPWRCHRSLIADALIVRGIQVEDIIGPR
                     GRSVRKLTAWSHVDGLQITYPPEEPGAS"
     gene            complement(2502..>3992)
                     /locus_tag="VIR04_08720"
     CDS             complement(2502..>3992)
                     /locus_tag="VIR04_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY9460870.1"
                     /translation="LHTRAPLQPYGKRTISTFSVAPGDRMTFTLSHQPSNEKPAVAFD
                     AQAALSETERFWHKFSDRCPDVGPWSGMVKRSLITLKALTYLPTGGIVAAATTSLPER
                     LGGERNWDYRYCWLRDATMTLLAFMSLGYFDEAAAWRDWLTRSVAGDPDQMQIMYGLR
                     GERRLPEYELSWLRGYEDSRPVRIGNAAALQTQLDVFGEVADAMVQAIKGKLPPHPRI
                     AAITEVIVPFLEKAWREPDEGIWEIRAPQRHFTHSKVMAWVTFDRIAIVAADMENGHA
                     FSENCRRIADEIHAEVCREGYDSELGTFVQYYGSKSVDASLLHIALTGFLPPDDPRVL
                     GTVAAIETHLMRDGLLLRYDSEVTEDGLPAGEGVFLACSFWLADVYVLLGRDDDARVL
                     FERLTGLCNDVGLLAEQYDPHGRRMLGNFPQAFSHVGIINTALNLHRALAPAKKRAHA
                     DSSNAELLNIDSHHAGEEQAEQTVLSADEATGPLADDQDAPGSSGG"
BASE COUNT          897 a         1187 c         1085 g          823 t
ORIGIN      
        1 cgcggtgcgt catttgccca tcgaacaaat acgttacggt cgacaggccg atgtgagggt
       61 gcggccgcac gtcagtctga accggaatat gaggcggaag gtcgtgcggc cccatacggt
      121 cgaaaaaaat gaagggtccg accatgcggc gtttgcgaaa cggcagcacc cggcccacct
      181 gaaaaccgcc aaggtcatgg cagcgggttt ctatgacgag atccagcatg gtggcgcccc
      241 ttttaagccg tgtgtttttt cacccaggca acatatttat ccatccagcc ttgcaggaat
      301 ttttggctgc ccgcgttggc gatattgcca gccgcatcga agaacgtgtc ggtaatatga
      361 ataaatacct cgggttgccc catcgttggt gaatccagat aagccaggac attacgcaaa
      421 tgctgttgag ccatggcgga tccgatggat ccgatggaag cgcccaatac acccgcgggc
      481 tgcccgatga aggcgccctg gccataggga cgcgatgcat tgtctatggc gtttttcagc
      541 acgccgggga tcgaccggtt atattcggcc gtgacaaaca gaagcccctg cgccgccttg
      601 acctcggctt tcagccgctt gacctgttca gcctgatttc cgtcgctgtc ctgattgtac
      661 aaaggcatat cgtcgattct cagttgttta aatgagaaat cgggcggccc caacttgatg
      721 agagcattag ccaatttacg gttgattgaa tctttacgca ggctgccgac aaaaacggca
      781 atttgatatt gagtcataac gatccttcag aaaattagaa gctgtgattc aggcccgcca
      841 tgtaacgcac agtagtaccc tggttttgca ccacttgatc atcaagcgaa taacgtttag
      901 ccacgcctgc catcgcataa agcctggacc gcgaagacaa gttgtatatg tagcctaatg
      961 tcgcaacatg gcccgttgcg gcgttttcgc catcttgcca cctccagtgc ggttttacca
     1021 cagaccactg agccaccacc gagctactgg cgccaacggg aatcgacaag cccgccaaat
     1081 acgcgtcaag gcgaccgcct cgaacaaatt ccgtggctcc aaggcccagt ccgagattat
     1141 caggatcccc gccgtccaga cccacataac cgtctttttg ccggctccag gctagcgaca
     1201 ccttggccac gccaaaatcg taagccgcgc ccagttgcca cgcctgaggg cgaaccggat
     1261 cccgcaacgc cggcgtcgac aggtaagctt tgtcccaggt tgccacagcc agcaaaggac
     1321 cgttttcata tttcagcgcg acactgctga ccggcgattt ccggccattg atctgaccgc
     1381 cgatcgcgtc aaaactgtat ccggcgccaa aactgaaacc ggcccatgac ggcgaaagat
     1441 aattgacggt attgtcaact tgataattgt ccgacgcctt gaatgttgca cccatgggca
     1501 tgtctttcca cgatgctatt tccagcaggc tgccaaactg ctgccccgca aggcgctggc
     1561 gcccgaaacg tatctcgcca aattgatcat tggccagtcc aacccaggca ttgctgtcga
     1621 agaaacggct cgggtccgcc agggaaccgg ttgtggcatc aatcatggac tccaattgaa
     1681 acgttgccag ccaaccgccg gctaaagcct cttgcccttt catgccgatc agggaatcgc
     1741 ttaagccgcc tgtggtcagt cctgtccttt gccgaggcgt tttttccgcg tcctgaaccc
     1801 gcgtatagct tatgccggta tcgaccacgc cgtacagggt gatcccgggc tcggcggcga
     1861 atgctgcgct ggcataggca atcaggccaa tagcggccat cagcacgagc cctcgaaatt
     1921 tggcggctac aattagcggc atggtggcga tcccggaaaa ccttaatgga tggaaaaact
     1981 gaaaatcaat caaacggccc cgttgtcgtc tgcacaatag gtcattcgaa ccggccgatc
     2041 gacgtatttc tggatctgct tcaaacgaac gaagtgactc acgtgcttga tgtgcgcacc
     2101 attccgcgat cgctgcataa cccgcaattc ggccaggata tgcttcctgc gcatcttcag
     2161 gcagccggta tcggctatac acatatggcg ggactgggcg gattgcggca cacaagccca
     2221 aactcgccca atggggcgtg gcgcaacgcg tcgttccgag gctacgccga ttacatgcaa
     2281 acggaggact tcgccgccaa tatcgagatt gtcattgagc ttgcgcgcaa agaacgctgt
     2341 gtgctgatgt gcgccgaagc cgtaccctgg cgctgccacc ggtcgcttat cgccgacgct
     2401 ctcatcgtgc ggggaattca agtcgaagat atcatcggac caagaggacg ctcggtgcgt
     2461 aaactcacgg cctggtcgca tgttgatggt ttgcaaatta cttatcctcc tgaagaaccc
     2521 ggcgcatcct gatcgtcagc cagaggcccg gttgcttcat cagcggacaa taccgtctgc
     2581 tcggcctgtt cctcaccagc gtggtgcgaa tcgatattca gcagctccgc gttggatgaa
     2641 tccgcatgcg cgcgtttttt ggccggggcc aaagcccgat gcagattcag cgccgtgttg
     2701 atgatgccaa cgtgactgaa cgcctgaggg aaatttccca acatgcggcg cccatgcggg
     2761 tcgtactgtt cggccaataa tccaacatcg ttgcacagtc ccgtcaggcg ctcgaacaaa
     2821 acacgggcgt cgtcgtcgcg ccccagcaag acatagacgt cagccaacca aaaagaacac
     2881 gccagaaata ccccttctcc cgcaggcaga ccgtcctcgg tcacttcgct gtcatagcga
     2941 agcagcaagc cgtcgcgcat caggtgggtt tcgatggccg caacagttcc cagcacgcgc
     3001 ggatcgtcgg ggggaagaaa gccggtcaag gcaatgtgca acaggctggc gtcgaccgat
     3061 ttggaaccgt aatactggac gaacgtgccc agttcgctgt cgtatccctc acggcaaact
     3121 tcagcgtgga tttcatcggc gatgcggcga cagttttctg aaaaggcatg accgttttcc
     3181 atgtcggccg caacaatggc gatgcggtcg aaggtgaccc acgccataac tttggaatga
     3241 gtgaagtgcc gctgcggcgc cctgatttcc caaatacctt cgtcgggctc gcgccacgcc
     3301 ttttccagga acggaacaat cacctcggta atggccgcga tacgcgggtg tggaggcagc
     3361 tttcctttaa tggcctgaac catggcatcg gccacctcgc caaatacatc caactgggtt
     3421 tgcagagccg cggcattgcc aatgcgcacg ggcctcgagt cctcgtagcc gcgcagccag
     3481 gaaagctcat actcgggcaa acgacgctcc ccgcgcagcc catacatgat ctgcatctgg
     3541 tccgggtcgc ccgccacgga acgagtgagc cagtcgcgcc acgcggccgc ttcatcgaaa
     3601 tagcccaggc tcatgaacgc caaaagcgtc attgtcgcat cgcgcaacca gcagtatcga
     3661 taatcccagt tgcgctctcc tcccaagcgt tcgggaagcg atgtggtggc cgccgcgacg
     3721 attccacccg tcggaaggta ggtgagggct ttcaaggtaa taagcgaccg cttcaccatg
     3781 ccgctccatg gccccacatc cgggcagcgg tcggagaatt tatgccagaa tcgctcagtc
     3841 tcggacaatg cggcttgcgc atcgaaggcc acggccggct tttcgtttga cggctgatgc
     3901 gaaagcgtga aagtcatgcg gtcgcccgga gcaacggaaa acgtactgat tgtgcgtttc
     3961 ccgtacggtt gcaatggcgc gcgtgtgtgc aa
//
LOCUS       DATCXU010000390         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_519417,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000390 DATCXU010000000
VERSION     DATCXU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519417"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="dctP"
                     /locus_tag="VIR04_08725"
     CDS             complement(<1..988)
                     /gene="dctP"
                     /locus_tag="VIR04_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY9460871.1"
                     /translation="MKRLFCVAATAAIMAASSAALAQTVTLRASHQFPGGQGDPRDEM
                     VQMIARSVEAANVGLKIQVFPSSSLYKPTEQWGALVRGQLDIASFPLDYASGRVPQFS
                     ATLMPGLVRNYAHAERLNKSPFMKDIKAIAEKQGAIVIADAWLAGGFASKKDCITAPP
                     TVKGQVMRAAGPAFEQMLAAAGASITSMPSSEVYTGMQTGVLDAVNTSNDSFISYRLY
                     EQVKCLTAPGENALWFMYEPIIMSKKSYDKLKPNQQKAIMDAAAKAETYFVGESKKGE
                     QKMVDAFTKAGVKVVTMSKDDYDQWMKLAEASSYKVFSEKVKGGDKLLKEALA"
     gene            complement(1189..1959)
                     /locus_tag="VIR04_08730"
     CDS             complement(1189..1959)
                     /locus_tag="VIR04_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9460872.1"
                     /translation="MTLDRQGAVALVTLSHPGRLNALTVSMWNELREIFTGVSADETL
                     RCVVLRGADGNFAAGADIREFPRERGDHASVVHYHMEIIAPALQAVARCIHPVIAQIE
                     GVCIGGGLEVASQCDLRIAASSSRFGVPINRLGFPMAPEEMRGLLDLAGRAVTMEILL
                     EGRVFGAQEALAKGLLTRVVPDADIAGDIARVTGRMARGAALAARLNKRIAARLCSRP
                     EPLSDSEIQSLFSYAESHDHREGVRAFLAGDEPTFSGD"
     gene            complement(2001..>3014)
                     /locus_tag="VIR04_08735"
     CDS             complement(2001..>3014)
                     /locus_tag="VIR04_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEY9460873.1"
                     /translation="LDPVTDQAARFFRRLNAQVDNLHFLVELRADMLRWRNQLAGLTV
                     LERELEILLSSWFDVGMLELKPITWDSPASLLEKLIQYEAVHEIRSWDELRHRVASHH
                     RCYAFFHPRMADVPLIFVEVAFASHMADNVQVLLDPKTPPEDLGKARWAIFYSISNTQ
                     TGLRGISFGNFLLKRVIDELLSELPRLKSFATLSPIPGFVDWLSKQDGKTLHPLLHDR
                     ADKQDALDNPQAGLKWVEHLQKAALGQGSEIYQRVGLKLATHYLTTMKGGRLVDPVAR
                     FHLGNGARMERVNWAADSSPKGLSQSCAMMVNYLYDLDQLDDNLASLAAGKPKLGRNL
                     RWW"
BASE COUNT          663 a          916 c          801 g          634 t
ORIGIN      
        1 ccgccaatgc ttcttttagc agcttgtcgc cgcccttgac cttctcggaa aaaaccttat
       61 aggacgacgc ttcggccagt ttcatccatt ggtcataatc gtctttgctc atcgtgacga
      121 cttttacgcc ggctttggtg aaggcatcga ccatcttctg ttcgcctttc ttggactcgc
      181 cgacgaaata ggtctcggcc tttgccgcag cgtccataat cgccttttgc tgattcggct
      241 tgagcttgtc gtaggacttc ttggacataa tgatgggctc gtacatgaac cacaacgcgt
      301 tttctcccgg agccgtgagg cacttgacct gttcgtaaag acggtaagag ataaagctat
      361 cgttggacgt gttgacggca tccagcacgc cggtctgcat cccggtatag acctcggacg
      421 agggcatgga cgtaatcgaa gccccagcag cggccagcat ttgctcaaac gccggccccg
      481 ccgcacgcat tacctgaccc ttgacagtcg gaggagcagt gatgcaatct ttctttgacg
      541 caaaccctcc cgccaaccag gcgtcggcga tgacaatcgc gccttgtttc tcggcaatcg
      601 ctttaatatc tttcatgaaa ggggatttat tcaatctttc ggcgtgggcg taattgcgca
      661 ccagacccgg catcagcgtc gcggaaaatt gcggtaccct gcccgacgca taatccagcg
      721 ggaaagatgc gatatcaagc tggccgcgca ccagcgcccc ccattgctcg gtgggcttat
      781 aaagcgatga cgacgggaag acctgaatct tcagacccac attggcggcc tcaacgctgc
      841 gggcgatcat ctgcaccatt tcatcgcgag ggtcgccttg cccaccaggg aactgatgcg
      901 aagcgcgcaa agtgacggtt tgggccaatg ccgcactgct ggcggccata atcgcggctg
      961 tcgccgcaac gcaaaatagt cgtttcatgg agtgtctcct gattaaagtt attcataacg
     1021 cccgcaaaat aaaccgggca tgtgccgcaa ccggcatttt gccagtacta ccaagtttcc
     1081 tttttatatg ttttgcaggc gcgttcgggc gtttgaatca catccgagcc ccgatcagtg
     1141 gaaaagcccg cgacaagcac agagtaaagg ttatcgttga tcactgcatt aatctcccga
     1201 aaaagtgggt tcatcgcccg ccagaaatgc gcgcacgccc tcgcgatgat cgtgactttc
     1261 ggcataactg aacaaggact ggatttcgct gtcgctcaag ggctccggcc ggctgcacag
     1321 ccgcgccgct atacgtttgt ttaaacgcgc ggccagcgcc gcgccacgcg ccatacggcc
     1381 cgtcacccgc gcaatgtcgc cggcaatatc cgcgtcgggc acgacccggg taagcagccc
     1441 ttttgccaat gcttcctgcg cgccaaacac gcggccttcg agcaggattt ccatggtcac
     1501 ggcacggccg gccaaatcca gcaaaccgcg catttcttcc ggcgccatcg gaaacccgag
     1561 cctgttgatg ggcactccaa aacgcgatga gctggctgca atgcgcaagt cgcactggct
     1621 ggcgacctcg agtccgcctc cgatgcatac gccttcaatt tgcgcaatga cgggatgaat
     1681 gcaacgggca accgcctgca aggcgggcgc gatgatttcc atgtggtaat gcaccacact
     1741 ggcgtggtca ccgcgctccc gcggaaattc ccgtatgtcg gcgcccgctg caaaattgcc
     1801 atcggcgccg cgcaacacaa cacaacgcaa ggtctcgtcg gccgacacgc cggtgaatat
     1861 ttccctgagt tcattccaca tcgataccgt caaagcatta agccgcccgg gatgggaaag
     1921 cgtgaccagc gccacagccc cctggcggtc aagcgtgatc ttgccggccg tgtcccgcgc
     1981 tggcccggtc tgccgccgat tcaccaccac ctcaggttcc gcccaagctt gggcttgccg
     2041 gctgcaagcg acgccagatt gtcatcaagc tggtccagat cataaaggta attcaccatc
     2101 atcgcgcagg actgcgacag tcctttaggg gaagaatcgg ccgcccagtt aacgcgttcc
     2161 atgcgtgcac cattgcctaa atggaaccgc gccacgggat cgaccagccg gccgcccttc
     2221 atggttgtca gataatgagt cgccagcttc aggcctaccc gttgatagat ttcagagccc
     2281 tggccgagcg ctgccttttg caagtgctcg acccatttaa ggccggcttg tggattgtcc
     2341 agcgcatctt gcttgtcggc ccggtcgtgc agcaatggat gcagcgtttt cccatcctgc
     2401 ttgctcagcc agtcaacaaa gccaggaatt ggcgacagcg tcgcaaacga cttcaggcga
     2461 ggcagctcgc tcaataattc gtcaatgacc cgcttaagca ggaaattccc aaagctgatg
     2521 ccgcgcaggc cggtttgagt gttggaaatc gaataaaaaa tggcccagcg tgccttgcca
     2581 agatcctctg gcggcgtctt cgggtcgagc aaaacttgca cgttgtcggc catatgcgac
     2641 gcgaaggcca cttcaacaaa aatcagcggg acatccgcca tgcgcggatg aaaaaatgca
     2701 tagcaacgat gatgactcgc cacccggtga cgcagctcgt cccatgatcg aatctcgtgt
     2761 acggcctcgt actgaatcag cttttccagc agcgatgccg gagaatccca ggtgatgggt
     2821 ttaagttcca gcatacccac gtcgaaccag ctcgataata aaatctcgag ttcgcgttca
     2881 agcaccgtca acccggcaag ctggttgcgc cagcgcagca tgtcggcgcg cagctcgacc
     2941 aaaaaatgca gattatcgac ttgcgcgttc aaccgtctga agaagcgtgc cgcttgatct
     3001 gtgacgggat caag
//
LOCUS       DATCXU010000391         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_858663,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000391 DATCXU010000000
VERSION     DATCXU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858663"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VIR04_08740"
     CDS             complement(<1..428)
                     /locus_tag="VIR04_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817737.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460874.1"
                     /translation="MHTFDYEIILRTAPYLFMVGMAFTLKLTVLSTIGAVILGTLLAM
                     MRLSSNRIVSGIAGSYVNFLRALPLILIIFWFYFLVPYIVGWATHAGRPISVGGFTSA
                     LITFTLFEAAYFCEIMRAGIQSIPRGQIGAGYALGLNYWQV"
     gene            complement(438..1178)
                     /locus_tag="VIR04_08745"
     CDS             complement(438..1178)
                     /locus_tag="VIR04_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460875.1"
                     /translation="MNYNWNWLIFWTPAADGTGTYLHTLIRGLYWTVGVSILAWIIAL
                     VMGILVGVLRTSRISWLEKICAGYVELFRNIPILVQMFLWYFVFPEVVPKTLGDFIKS
                     ADPVWGTFWTAVICLGLFTSARIAEQIRAGVNSLPAGQPMAATALGLTRPQVYRYILL
                     PVTMRIIMPPLTSEALNLIKNSSIAFTIGLLEITGAARSMQEFTFQIFESFAAATVLY
                     LVINAVVVVLMSYIEKWISVPGFIGAER"
     gene            complement(1264..2115)
                     /locus_tag="VIR04_08750"
     CDS             complement(1264..2115)
                     /locus_tag="VIR04_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9460876.1"
                     /translation="MSTVQAEGRLDKIKTTGEITLGYRDSSIPFSYLDDHQQPIGYSM
                     EICHRIVDAVKKDLKLPTLKTKLVPVTSSTRIPLVANGTVDISCGSATNNQQRQEQVS
                     FAPTTFVTATRFAALKKSNLKDLADFKGKTVVSTSGTSNLKWLTETNASQNLGMKIIA
                     AKDHSEAFLTVGNGRASAFFMDDILLAGLVATSRNPSDWMINDKAYTIEPYGLIEPKG
                     DPAFKKVVDDAVKGLMKDGSIEKIYAKWFTKPIPPKNVNLNWPISPELKKTFAHPTDS
                     FDPAVYK"
     gene            complement(2355..>2683)
                     /locus_tag="VIR04_08755"
     CDS             complement(2355..>2683)
                     /locus_tag="VIR04_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY9460877.1"
                     /translation="AIIKPFKLDEVRDALAEVGVSGLTVTEVKGFGRQKGHTELYRGA
                     EYVVDFLPKIRIDVVLPATMVETAIDAIVKAAFTGKIGDGKIFVTAVEQAIRIRTGES
                     GTDALS"
BASE COUNT          696 a          708 c          675 g          604 t
ORIGIN      
        1 acttgccagt aattcaggcc cagagcgtag ccggcgccaa tctggccgcg cgggatggac
       61 tgaattccgg cgcgcatgat ttcgcaaaaa taagcggctt caaataacgt aaacgtaatt
      121 aatgcggaag taaatccgcc caccgatatg ggccgcccgg cgtgcgtcgc ccagccgacg
      181 atataaggaa ccaaaaaata aaaccagaag atgatcagaa tcaatggcag agcacgcagg
      241 aaattgacgt aactgcctgc aattcccgac acgatgcgat tggacgacag acgcatcatg
      301 gccagcagcg tgcctaaaat gacggcgccg atcgtggaca acaccgtcag ctttaacgta
      361 aacgccatac ccaccataaa aagataggga gccgtgcgca agataatttc gtagtcaaat
      421 gtgtgcatgg cgcctcctta tctctcggcg ccgataaagc cgggcacact tatccacttt
      481 tcgatatagc tcataagaac gacaaccacc gcgttaatga ccagatacaa aaccgtcgcc
      541 gccgcaaacg attcaaaaat ttgaaaagta aattcctgca tggagcgcgc cgccccggta
      601 atttcaagca ggccaatcgt aaaggcaatt gacgagttct ttattaaatt cagcgcctcg
      661 gaagtaagcg gcggcatgat aatgcgcatg gttacgggca acaatatata gcgatatacc
      721 tgcggccggg tcaaacccag cgcggtggcg gccatgggct gcccggctgg caaagaatta
      781 acacccgcgc gaatttgctc ggctatgcgc gccgaagtga ataaacccag acaaataacg
      841 gccgtccaga acgtgcccca gacaggatct gcggatttga tgaaatcgcc gagcgttttt
      901 ggcacaacct cgggaaagac aaaataccac aagaacatct gcaccaatat tggaatattg
      961 cggaacaatt caacatagcc ggcgcaaatt ttttcgagcc atgagatgcg gctggtgcga
     1021 agcactccaa ccagtatgcc catgaccagt gcaatgatcc aggccagaat cgatacccct
     1081 acagtccagt aaaggccgcg tatcagggta tgaagataag ttccggttcc gtcggccgcc
     1141 ggcgtccaga aaattagcca gttccagttg taattcatac ttggcctcgt gaccgcgatc
     1201 gggcttgatt aaataaaacg gcacaatccg cgcaaagccg gattgtgccg cgcacgcaaa
     1261 aaactatttg taaaccgctg ggtcaaaaga gtcggtcgga tgagcaaacg ttttcttcag
     1321 ctccggcgat atcggccagt tcaaattgac gtttttggga ggaatgggtt tggtaaacca
     1381 tttagcataa atcttttcaa tggaaccgtc cttcattagg cctttgaccg catcgtcaac
     1441 cactttcttg aatgcggggt cgcccttggg ctcaatcagg ccataaggtt cgatcgtgta
     1501 ggctttgtcg ttgatcatcc agtcggatgg gttgcgcgac gtcgctacca aaccggccag
     1561 cagtatgtcg tccatgaaga atgcggaagc ccggccgttg ccgactgtca ggaacgcttc
     1621 ggaatggtct ttggccgcaa taattttcat gccgaggttc tgcgaggcgt tggtttcggt
     1681 caaccatttc aggttgctgg ttcccgaagt cgacaccacc gttttaccct tgaaatcggc
     1741 caagtctttg agattcgact ttttcagggc cgcaaaccgt gtggcagtaa caaaggtggt
     1801 cggagcaaag ctgacctgct cctggcgctg ctgattgttc gtggccgatc cgcacgaaat
     1861 atcaaccgtg ccattggcca ccagcggaat gcgagtcgat gacgtgacgg gcaccagctt
     1921 ggtttttaac gtgggcaatt tcaagtcttt tttcaccgcg tcgacaatgc ggtggcatat
     1981 ctccatcgaa tagccgatgg gctgctgatg gtcgtccagg tacgaaaatg gaatggatga
     2041 gtcgcgataa ccgagtgtga tctcacccgt agtcttgatc ttgtcaagac ggccttcagc
     2101 ctgcaccgta ctcattccaa cggctgttac ggccacagct aaaaagtagg gcaacagttt
     2161 catggtatct ccttcaaaat tgattgggcg ctctgcgaca aagccccggc ttttacagtt
     2221 tcgcacaaat gcacacagaa acactatttt tttgcccggc tggccggacg cttaaaaagt
     2281 gttacgaagg tggcagtatg cctgattatt tccgaacttt ccgtgcagaa ataccctaag
     2341 taaattagct gaagttatga aagagcatct gtgccgcttt cgccggtgcg aatgcgtatg
     2401 gcctgctcga cagccgttac aaaaatcttg ccgtcgccaa tcttgccggt aaaagccgct
     2461 tttacgatcg catcaatggc ggtttcgacc atcgttgcgg gcaacaccac gtcaatgcga
     2521 atctttggaa gaaaatcaac gacgtattca gctccgcgat aaagctcggt atgccccttt
     2581 tgtcgcccga atcctttcac ctcggtgact gtcaagccgc taacccccac ttccgccagc
     2641 gcgtcgcgca cttcatcaag cttgaacggc ttgataatcg ccg
//
LOCUS       DATCXU010000392         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_905357,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000392 DATCXU010000000
VERSION     DATCXU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905357"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VIR04_08760"
     CDS             complement(<1..515)
                     /locus_tag="VIR04_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY9460878.1"
                     /translation="MNHTEEPIVTTIDGHGSAIKTPKQLLITVILAFAVPITIIMMLV
                     HLVASETKVGAGSDAKTPSSIQSRIEPVAGLKLVDASAPKVFQTGQQVFETTCTACHT
                     AGVAGAPKFGDKAAWAPFIKAGYAELVKNAIHGIGAMPPKGGNTSLSDFEVERAVVYM
                     TNKSGASFKEPA"
     gene            755..829
                     /locus_tag="VIR04_08765"
     tRNA            755..829
                     /locus_tag="VIR04_08765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:788..790,aa:Arg,seq:ccg)
     gene            complement(850..1248)
                     /locus_tag="VIR04_08770"
     CDS             complement(850..1248)
                     /locus_tag="VIR04_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY9460879.1"
                     /translation="MTQYLLDTNAVIAILKAPNGPVSQCLRKHGPQDVAVSSLVMHEL
                     YYGAFHSQRVEHNLAIVDGLQFEVLEFDQEDARHAGEIRAQLAQSGTPIGAYDVLIAG
                     QARARSIVLITRNVREFSRVSELSIENWEK"
     gene            complement(1260..1508)
                     /gene="vapB"
                     /locus_tag="VIR04_08775"
     CDS             complement(1260..1508)
                     /gene="vapB"
                     /locus_tag="VIR04_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY9460880.1"
                     /translation="MATTTVKLFWSGRSQAVRLPKAFRFDSEQVRIRQHGAGLILEPV
                     PESWSWLDKLSAPDRDFTDAATDQPGIQQRDDLDKLFE"
     gene            1800..3431
                     /gene="ggt"
                     /locus_tag="VIR04_08780"
     CDS             1800..3431
                     /gene="ggt"
                     /locus_tag="VIR04_08780"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY9460881.1"
                     /translation="MVVTDQHLASEVGLKILKEGGNAIDAAVAVGYTLAVVDPCCGNI
                     GGGGFMTLHLADGRNIFVNFRERAPLKATRDMYLNDKGEVAPGRSTRGYLAVGVPGTV
                     MGLDTVLQKYGTMTRKQVMEPAIKLAEEGFVLQRGDVDIMTGSGNRGDLDALSGPSKV
                     FAEEPNIAAIFLKDGKPYKPGDRLVQTNLAKTLSLIEKDGPDAFYKGPIADAIVRASD
                     THGGILGKADFESYRVEQTKPVECDYRDYRVISSPPPSSGGTTLCEILNIIEAYPLGK
                     MGFHSAAATHVMVEAMRHAYLDRNTLLGDPDFVKAPVARLISKDYAARIRAAIQPDKA
                     TPSDELKQGTAPHEGDNTTHYSVVDVKGNAVSTTYTINSYFGANVMAGDTGFFLNNEM
                     DDFTSKVGTANLYGLVQGSNNAIEPGKRPLSSMSPTVALKKGQLSMVFGSPGGSRIIT
                     TALETFVNVVDYGMNIQEAVDAPRFHHQWQPDVIQHEPYAFSQDTIDKLATMGYRFKS
                     YGPWGAAEAILVEPATGMLTGSTDNRHPAGAAAGY"
BASE COUNT          765 a          901 c          983 g          784 t
ORIGIN      
        1 gcgggttcct taaagctggc gccggattta ttggtcatat agacgacagc gcgttcgact
       61 tcaaaatcgc ttaacgacgt attgccgccc ttgggaggca ttgcgccaat accgtgtatg
      121 gcgtttttaa cgagctcggc atagcccgct ttaatgaatg gcgcccatgc ggctttgtcg
      181 ccgaactttg gcgcgccggc aacacccgcg gtgtggcagg cggtacaggt ggtttcgaac
      241 acttgctggc ctgtttgaaa taccttgggc gcgctggcat caaccagctt gagccccgct
      301 acaggctcta tgcgggactg aatggagcta ggagttttgg cgtcggaacc ggcgcccact
      361 ttggtttcag atgcaaccag gtgaacgagc atcatgatga tcgtaatggg aaccgcgaac
      421 gccagaatga ctgttataag gagctgcttg ggcgttttaa tggcgcttcc atgcccatca
      481 atggtcgtta ctatgggttc ttcagtatgg ttcataatcg gatcctgcgt attcgtgatc
      541 aaaggcgatc gaagcctgaa ttctggtgcg cggtgcaata aacaacctgc cccgtataac
      601 ggggcattgc gcttttgaac cgcctcggtt cgagattata acgagactgc ggtcagactg
      661 cagaaatgcc catatgcgaa taacggattg attcgcatgt tcgtattccc atgattgcgg
      721 aaatctggaa taaacacgct acaatgacgg ccctgcgccc gtagtttaat ggatagaatg
      781 agagcctccg aagcttttga tacaggttcg attcctgtcg ggcgcaccag acacatcagt
      841 gggcgtccat tatttttccc agttttcgat gctcagttcc gacacgcgcg aaaactcgcg
      901 gacattgcgc gtaatcagca ctatgctccg cgcgcgggcc tgccccgcga tcagcacatc
      961 gtaggccccg ataggtgttc ctgattgtgc taattgcgcc ctgatttcac ccgcatgacg
     1021 cgcgtcttcc tggtcgaatt ccagcacctc gaattgaagg ccgtccacga tagccaggtt
     1081 gtgctccacg cgctgactgt gaaaagcgcc atagtacagt tcgtgcatca ccagcgatga
     1141 cacggcgacg tcttgcggac catgcttccg caggcattgg ctgacaggcc cgttgggtgc
     1201 tttcagaatt gcaatgactg cattcgtatc aagcagatat tgggtcatgc tggaagggat
     1261 tattcgaaca atttatcgag atcatctcgc tgctgaatgc cgggttgatc cgtggcggcg
     1321 tcggtaaaat cccggtcggg cgcggaaagc ttatcgagcc atgaccagct ttcaggtacg
     1381 ggttcaagga tgagcccggc gccatgttgc ctgattctga cctgttcgct atcgaaccgg
     1441 aatgctttgg gcagacgcac cgcctgcgaa cgccccgacc aaaaaagttt tacggtggtc
     1501 gtggccatgt ccgtctcctg gatggtatat gacattgtat tataccaagc tgattccgga
     1561 aggcaatgca gatcgaagca acttaatatt ggtaatagag gccaaagcct ttatgatttg
     1621 ttcgttgtgt cttggttgga gaacgagcaa tgagcacaat caaagttagc atgacttggc
     1681 gcttcctcaa gggaacagtt cagtcgggcc gctatgtgtt ggcgatggta ctgctggcgc
     1741 tggccggtgc gccgcttgca ttggcggttt caccgccacc cgcctatggc aagcacggca
     1801 tggtcgtcac ggatcagcat ttggcgtcgg aggtcggcct caagattctc aaggaaggag
     1861 gcaacgcgat tgatgccgcc gttgcggtgg gatatacgct tgccgtggtc gacccctgct
     1921 gcggcaatat cggcggtggc ggcttcatga cgctgcattt ggccgatggc cgcaatattt
     1981 ttgtcaattt tcgcgagcgg gcgccgttga aggcaacgcg cgacatgtac ctgaacgaca
     2041 aaggcgaagt tgcgccgggg cgcagtacac gcggctatct ggcggttggc gtccccggca
     2101 cagtaatggg gcttgacact gtgttgcaga aatacggcac gatgacgcgc aagcaagtga
     2161 tggaaccggc tatcaagctt gccgaagaag gcttcgtgct gcagcgcggc gacgtcgaca
     2221 ttatgaccgg cagcggcaat cgtggtgacc tcgatgcgtt aagcggcccc agcaaagtgt
     2281 ttgcggaaga acccaacatc gcggccattt tcctgaaaga tggcaagcct tacaagccgg
     2341 gcgaccggtt ggtgcaaacc aatttggcga aaaccttgtc gctgattgaa aaagacggcc
     2401 cggacgcgtt ctacaaaggt ccgattgcag acgccattgt cagggcaagc gatacgcacg
     2461 gcggtattct tggcaaggcc gatttcgaga gctaccgagt cgagcagacc aaaccagtag
     2521 agtgcgatta ccgtgattac cgcgtaattt catctcctcc accgagctcg ggtggcacaa
     2581 cgctgtgcga gatattgaat atcatcgaag cctatccgct gggcaaaatg ggtttccatt
     2641 ctgccgctgc gacgcatgtt atggtcgagg ccatgcggca tgcttatctt gatcgaaaca
     2701 ccttgctggg cgatcctgat ttcgtaaagg cgccggttgc gcgattaatc tcgaaggact
     2761 atgccgcgcg gatccgcgca gcgatccagc ccgataaagc gacaccatca gatgaactaa
     2821 agcagggaac agctccgcac gaaggcgaca acactacgca ttattcagtc gtggacgtca
     2881 agggcaatgc ggtgtcaacc acatacacga tcaacagcta ttttggcgcc aacgtgatgg
     2941 ccggcgatac cgggttcttt cttaataacg agatggacga tttcacctcc aaggtcggca
     3001 cggccaatct gtatgggttg gttcagggat cgaacaatgc cattgagcca ggcaaacgtc
     3061 cgcttagctc catgagtccg actgtcgcgc tcaagaaagg ccaactgtcc atggtttttg
     3121 gcagcccggg gggttcgcgc attatcacga ctgctctcga gactttcgtc aatgtggttg
     3181 attatggaat gaatattcaa gaagcggtcg acgcgccgcg ttttcatcat cagtggcaac
     3241 ccgacgtgat tcagcatgaa ccctatgcct tctcgcagga caccatcgac aagctcgcga
     3301 ctatgggcta tcgcttcaag tcatatggcc catggggcgc tgccgaggcg attttggtgg
     3361 aaccggccac cggaatgttg actggcagca cggacaatcg ccatccggcc ggcgcggcgg
     3421 caggttattg acc
//
LOCUS       DATCXU010000393         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_125889,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000393 DATCXU010000000
VERSION     DATCXU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125889"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VIR04_08785"
     CDS             <1..312
                     /locus_tag="VIR04_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY9460882.1"
                     /translation="KRVAANVKQAMVVPGSGLVKRQAEQEGLDKIFLAAGFEWREPGC
                     SMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIAGHFVDVRNF
                     R"
     gene            322..972
                     /gene="leuD"
                     /locus_tag="VIR04_08790"
     CDS             322..972
                     /gene="leuD"
                     /locus_tag="VIR04_08790"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY9460883.1"
                     /translation="MQAFITHKGLVAPLDRENVDTDLIIPKQFLKSIKRTGFGPNLFD
                     ELRYLDHGEPGKDNSRRPLNPDFVLNQPRYQGASILLARKNFGCGSSREHAPWALGQY
                     GFRAMIAPSFADIFFNNSFKNGLLPIVLSEPEINRLFDEVNAFVGYQLNIDLPRQRVV
                     CPDGRELRFDIEPFRKHSLLNGLDDIATTLQKSDQIRAFEAEHLARYPWLVGQPAR"
     gene            1089..2168
                     /gene="leuB"
                     /locus_tag="VIR04_08795"
     CDS             1089..2168
                     /gene="leuB"
                     /locus_tag="VIR04_08795"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY9460884.1"
                     /translation="MAHKIAVLPGDGIGPEVTEQALRVLRALAPDIEVQSAPVGGAAF
                     DACGHPLPASTLELAKNSEAILFGAVGDWKYDQLARELRPEQAILGLRKALGLFANLR
                     PAILYPQLANASSLKPEIVAGLDILIVRELTGDIYFGQPRGVRELTDGPFKGERQGFD
                     TMHYAESEVRRIARVAFQAAQKRSKKLCSVDKANVLETSQFWRETMIDVARDYPDVAL
                     SHMYVDNAAMQLVRAPKAFDVIVTGNLFGDILSDEASMLTGSIGMLPSASLNASNQGL
                     YEPSHGSAPDIAGKNVANPLATILSAAMLLRYSLNLPALANRIESAVQTVLEQGLRTA
                     DIAEAGAVTVSTTAMGDAVLRALAA"
     gene            2247..3377
                     /gene="asd"
                     /locus_tag="VIR04_08800"
     CDS             2247..3377
                     /gene="asd"
                     /locus_tag="VIR04_08800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY9460885.1"
                     /translation="MKQAVGLVGWRGMVGSVLMQRMREENDFSLFEPVFFSTSNVGGA
                     APKWAVGAAALSDAYDIDALKKLPIIVTAQGGDYTTKVYPKLRAAGWNGVWIDAASTL
                     RMADDAIIVLDPVNRPVIDAALQRGIKNYIGGNCTVSCMLMGLSGLFNQDLVEWMTSM
                     TYQAASGGGAQHMRELLVQFGMINSAVKSLLDDPAAAILEIDRGVLATQHDADLPQDN
                     FGVPLGGSLIPWIDKDLGSGVSREEWKAGAETNKILGRGEAFGTKPTPIDGLCVRIGA
                     MRCHSQALTIKLKRDVPLDEIADILRQGSKWAKIVPNEREASMRELTPVAVTGTMDVP
                     VGRLRKMSMGPDYLSAFTVGDQLLWGAAEPIRRMLRITLGEL"
     gene            3386..>4450
                     /locus_tag="VIR04_08805"
     CDS             3386..>4450
                     /locus_tag="VIR04_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY9460886.1"
                     /translation="MSRRLAIPALALHVRALVIALSTAMLTCTTAYAANFGHSRIVSN
                     PGQPLGIDVPITQLNADDLRSLVVRPASTLAWNQAGLTPPVPLNTMQVRVVDGFRPDA
                     KVIQVRSTQAFSGPVADLLLNVSTASGQQQYQVSIVAPTPSGAFGATGAVQAGAAAPG
                     AHSRAKRAQGAIRIRHGDTLWAIARRHAVPGVSIFQMMVALQRANPQAFIHQNLNLIK
                     AGATLTIPDRAALTAVSDREARRIFEQQLHDFMKYRQGVASRGANLVRGEAANKGQVL
                     PSTPSAAPVQPEGPRDQLKLSTARSAADKAADDRVATEKGIAESQARVSTLEGNVKDL
                     NQALQSQGRAASDALIGGAKG"
BASE COUNT          917 a         1178 c         1361 g          994 t
ORIGIN      
        1 aagcgcgtcg ccgccaatgt caagcaggca atggtggtgc cgggctcggg tctggtcaaa
       61 cggcaagccg aacaagaagg tttggacaag atttttcttg cggccggctt cgagtggcgt
      121 gaaccgggct gttcaatgtg ccttgcgatg aatgccgacc gacttgaacc gggtgaacgg
      181 tgcgcatcga cctcgaaccg caatttcgag gggcgccagg gacagggcgg ccgcacgcat
      241 ttggtcagcc cggcaatggc tgcagccgcc gcgatagccg gtcattttgt cgatgtcaga
      301 aattttcggt aggaaatcat tatgcaagca tttatcactc acaagggcct tgtggccccg
      361 cttgaccgcg aaaatgtcga cactgatctg attattccga aacagtttct gaaatcgatc
      421 aaacgcactg gcttcggtcc taatctgttt gatgagttgc gctacctgga tcatggcgag
      481 ccgggcaaag ataactccag gcggcccctg aaccccgact ttgtgttgaa ccagccgcgt
      541 taccaggggg cgtcgattct gctggcgcgc aaaaattttg gctgtggctc cagtcgcgaa
      601 cacgctccct gggcgttggg ccagtatggc tttcgcgcca tgatcgcccc gtcttttgcc
      661 gatattttct tcaacaacag ttttaaaaat ggcttgttgc ccattgtgtt atccgaaccc
      721 gagattaatc gcttgttcga tgaagtcaac gcctttgtgg gttatcaatt gaatatcgat
      781 ctgccgcgtc aaagagttgt gtgtccggac ggccgcgagc tgcgcttcga tatcgaacct
      841 tttcgcaagc acagcttgct aaacggattg gacgatattg cgaccacgtt gcagaagtcc
      901 gaccagattc gcgcattcga ggccgagcat ttggcgcgtt atccgtggct ggtggggcag
      961 ccggctcgtt aatgcgaaca ggtgggcgcg agtttgcaaa agcgtcataa aaacgggggc
     1021 cggtaatacg gcccttgccg tattaccggc tttgaaattt atgtggatta cctttttgga
     1081 attgatttat ggctcataaa attgcggttt tgccgggcga cggcataggt cccgaggtca
     1141 ctgaacaagc gctgcgcgta ctgcgcgcgc tggctccgga cattgaagtg caatccgcgc
     1201 ctgttggggg cgcagccttc gacgcctgcg gacacccgct accggcttcg acacttgagc
     1261 ttgccaaaaa ttcggaagcg atcctgtttg gcgccgtggg cgattggaaa tacgatcagc
     1321 tggcacgcga gctgcggcca gagcaggcta ttttaggctt gcgcaaggcg ctgggtcttt
     1381 tcgccaattt gcggccggcg attttgtatc cgcaattagc caatgcgtcg tccctgaagc
     1441 ccgagatcgt ggccggactg gatattttga ttgttcgcga actgaccggc gatatttatt
     1501 ttggacagcc gcggggcgtg cgcgaattga ccgatggacc atttaaagga gagcgacagg
     1561 ggttcgacac gatgcattac gcggaatcag aagtgcggcg catcgcccgt gtcgcgttcc
     1621 aggcggcgca aaagcgcagc aagaagttat gcagcgtgga caaggcaaat gtgctggaaa
     1681 cgtctcagtt ctggcgtgaa acgatgatcg atgtggcgcg tgattaccct gatgttgcgc
     1741 tttcgcacat gtatgtcgat aacgcggcga tgcagttggt gcgcgcccct aaagcgttcg
     1801 acgtcatcgt gaccggtaat ttgttcggcg acattttgtc tgacgaggcc tctatgctga
     1861 ccggctcaat cggcatgttg ccgtccgcat cgctgaacgc gtcgaatcag ggtttgtatg
     1921 agcccagcca tggctcagcc cccgatattg cgggtaaaaa cgtcgctaac cccttggcga
     1981 caatattgtc cgccgccatg ttattgcggt attccctgaa tctgcccgcc ttggccaatc
     2041 gaattgagtc ggctgtgcag actgtgcttg agcagggcct gcgcacggcc gatattgccg
     2101 aggccggtgc tgtaacagtt tcaacgaccg cgatgggcga tgctgtttta cgagcattgg
     2161 ctgcataata atcgggggtt tgggcgcgat tccggtaaaa tgccgcttaa agtccgtttt
     2221 ttattattct ttcgagcggt ggtgaaatga agcaggcagt gggtttggtt ggttggcggg
     2281 gcatggtggg ttcggttttg atgcagcgga tgcgtgaaga aaatgatttt tcgttgtttg
     2341 agcctgtgtt tttttcgacc agcaatgtgg gcggggccgc tcccaaatgg gccgtcggcg
     2401 cggcagcgct aagcgacgcc tacgatatcg acgccctgaa aaagcttccc atcatcgtta
     2461 cggcgcaggg cggcgattac accaccaagg tatacccgaa gctgcgcgcg gccggatgga
     2521 acggggtatg gattgacgcc gccagcacgc tacgcatggc cgacgacgcc attattgtgc
     2581 tcgacccggt caatcgcccg gttatcgacg cggcgttgca gcgcggcata aaaaactata
     2641 ttggtggcaa ttgcacagtt agctgcatgc tgatgggtct gtccggcttg ttcaatcagg
     2701 atctggttga atggatgacc agcatgacct accaggcggc gtcgggcgga ggcgctcagc
     2761 acatgcgtga actgctggtt cagttcggaa tgatcaatag cgccgtcaag tccctgcttg
     2821 atgatccggc ggcggcaatt ttagaaattg atcgcggtgt tttagcgacg cagcacgacg
     2881 cagacttgcc gcaagacaat ttcggcgtgc cgttgggggg cagtctgatt ccgtggatcg
     2941 ataaagatct tggcagcggc gtttcgcgcg aggaatggaa agctggcgcg gaaacgaaca
     3001 aaatactggg ccggggcgaa gccttcggca cgaaacctac gcctatcgac ggattgtgtg
     3061 tgcgtatcgg cgccatgcgt tgccacagtc aggcgctcac catcaaatta aaacgcgacg
     3121 tcccgctgga cgagattgcg gacatactgc gccaaggttc caaatgggcg aaaattgttc
     3181 ccaacgagcg cgaagccagc atgcgcgaac taacccctgt ggcggttacc gggacgatgg
     3241 acgtgccggt ggggcgttta cgcaagatgt caatgggccc cgactacctt agcgcgttta
     3301 cagtgggcga tcagctatta tggggcgcgg ctgaaccaat acgccgcatg ttgcgtataa
     3361 ctttgggtga gctctgaaaa tgcgaatgtc ccgtcgtttg gccatacctg ccttggcgct
     3421 ccatgtcagg gcgctggtta ttgctttatc gactgcgatg ctgacatgca ccaccgccta
     3481 tgcggcaaac ttcggccatt cgcgcattgt ttccaatcct ggccagccgc tgggcattga
     3541 tgtgcccatt acgcaattga atgcggatga tttgcggtcg ctggtcgtgc gtccggcatc
     3601 cacgctggca tggaatcagg cgggtctgac gcctcctgtt ccacttaata ctatgcaggt
     3661 gcgcgttgtc gacggattca ggcccgacgc gaaggtcata caggttcgct ctacccaggc
     3721 atttagcggc ccggtagccg acctgttgct aaacgtaagc actgcgtcgg gacagcagca
     3781 atatcaggtc agcatcgtcg cgccgacgcc atccggcgct ttcggcgcga cgggcgcggt
     3841 acaagccggg gcggcggctc caggggctca ctcacgcgcg aagcgcgctc aaggcgcgat
     3901 ccgcatacgg cacggcgata cactgtgggc cattgcacgc cgccatgctg tgccgggcgt
     3961 cagtattttt cagatgatgg tggcgttgca acgcgccaat ccgcaggcgt tcatacatca
     4021 gaatttgaat ttgattaaag ccggcgcaac cctcacgatt cccgacaggg cagcattgac
     4081 ggccgtcagc gatcgcgagg cgcgtcgtat tttcgagcag caattgcatg attttatgaa
     4141 gtaccggcaa ggggtggcat ccaggggcgc caatcttgta cggggcgaag ccgccaataa
     4201 aggccaggtt ttgccttcga caccttcggc agcgccggtc cagcccgagg ggccgcgcga
     4261 ccagcttaaa ctgtcaacgg cacgttcggc ggcggacaag gcggccgatg accgtgtcgc
     4321 aaccgaaaaa ggcattgccg agtcacaggc gcgggtatcc acgctcgagg ggaacgttaa
     4381 ggacctgaat caagccctgc aatcgcaggg gcgggcagcg agcgatgccc tcattggcgg
     4441 ggctaaaggc
//
LOCUS       DATCXU010000394         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1139524,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000394 DATCXU010000000
VERSION     DATCXU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139524"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VIR04_08810"
     CDS             <1..312
                     /locus_tag="VIR04_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY9460887.1"
                     /translation="NGTNALALAQLQTAKTLGNGAMSLNEAYSQVVNKVAVLTQQNTT
                     ASAAQTALIQQNTAAQQAVSGVNLNEEYVNLDNYQQQFQAASRLITVSSTLFQALLSM
                     G"
     gene            363..1607
                     /gene="flgL"
                     /locus_tag="VIR04_08815"
     CDS             363..1607
                     /gene="flgL"
                     /locus_tag="VIR04_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY9460888.1"
                     /translation="MRISSSQLFQTGLNAINAQQADVMHVYQQIASGQRMVTPADDPL
                     AASQAINIAQSQSLNQRFAANRDVASRNLGTEDNVLGTVTTQMQAIKASLVQAGNGAL
                     SDVDRATLADTLSSAKSSLLTLANSTDDNGQYLFSGSQGTQAPFVADASGTVTATLGV
                     SGQRLIQVDPTRQMASSDLGADIFVRATPGTQVYLTAAASGNTGTGQIGTPTVTDPLG
                     ANVGKNFSISFNGSPLQYTINMTDAAGVAAGSVGPLAYQANSTMLDLTGGVQIQFSGQ
                     PSAGDTFTVDRAVPANLNVFSTLDDAIAALKAPSAGNAAASVALSNALSTAIQKTSVV
                     YDNVLAVRASVGSRMNEIDALNASGTQRDVGYSTQLSQLESVDYYAASTQLQLRQSAL
                     DAASLAFKKIQGNSLFNMGNGG"
     gene            complement(1632..1955)
                     /locus_tag="VIR04_08820"
     CDS             complement(1632..1955)
                     /locus_tag="VIR04_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEY9460889.1"
                     /translation="MSTSSPILNQYQIIASLSSQILAEARLSHWDPLTSLARQYQLAV
                     EALRHMNQLSSEDRAARQPLLAQILDDDARLRNLAAPELKRLGALLGQTKGQRSVSRA
                     CGAHS"
     gene            complement(1996..>2442)
                     /gene="fliS"
                     /locus_tag="VIR04_08825"
     CDS             complement(1996..>2442)
                     /gene="fliS"
                     /locus_tag="VIR04_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY9460890.1"
                     /translation="HAKIIEEEETMYRSKQSGVRAYAQIGLEIGAATASPHKLIVMLF
                     DGALLAIATALQCMNTGDIANKGKSVSFAISIIDNGLRASLDKEAGGDIAKNLDALYD
                     YMTRRLLMANMKNEPEGLQEVHRLLNELKEAWQAIEAPEPAAMASV"
BASE COUNT          523 a          683 c          688 g          548 t
ORIGIN      
        1 aacgggacca atgcgcttgc cttggcccag ttgcaaacgg ccaagacgtt gggcaacggc
       61 gccatgagtc tgaacgaagc gtattcgcag gtagtgaaca aggtcgcggt tctaacccag
      121 caaaatacca cggcatccgc agcccaaacc gcgctcattc aacagaacac ggcagcccag
      181 caagccgtgt cgggcgttaa tttaaacgaa gaatatgtca acctcgataa ttatcagcag
      241 cagtttcagg ccgcatcgcg cctgatcacc gtcagctcca cgttgttcca ggcattgttg
      301 tccatgggct agcccgcagg cttgttgcga aggttaacca gaattttttg cgaggcgccg
      361 ccatgcgtat cagttccagc caactttttc agaccggatt aaatgccatc aacgcccaac
      421 aggctgacgt gatgcatgta tatcagcaga ttgccagcgg tcagcgcatg gtgacaccgg
      481 ccgacgatcc cctggccgca tcgcaggcca tcaatattgc gcaatcgcaa tcattgaacc
      541 agcgctttgc ggccaatcgc gacgtggctt cgcgcaatct gggcaccgaa gataatgtgc
      601 ttggcacggt aaccacgcag atgcaggcta tcaaggccag tctggttcag gccggcaatg
      661 gcgcgctgtc cgatgtggac cgcgccacgc tggccgatac cctgtccagc gccaaaagca
      721 gtctgttgac gctggcaaac agcaccgacg acaatggcca gtatcttttt tcaggttcgc
      781 aaggcacgca agcgcccttt gttgctgatg catccggaac agtgactgcg acattgggcg
      841 ttagcggtca gcgtctgatt caggtcgatc ccacccgcca gatggcaagc agcgatcttg
      901 gcgccgatat ttttgttcgg gctacgccgg gaacccaggt gtatttaacc gctgcggcat
      961 ccggcaatac gggtacgggt caaattggca caccaacggt taccgacccc cttggagcca
     1021 atgtcggcaa gaatttttca atttcgttca acggttcgcc gttgcagtac acgataaaca
     1081 tgaccgacgc ggccggtgtt gccgccggtt cggtcggacc gcttgcgtat caggcgaaca
     1141 gcactatgct ggacttgacg ggcggggtcc aaattcagtt ttccgggcaa cccagcgcgg
     1201 gcgacacatt taccgtggac agggcagtcc cggcaaatct taatgtattc agtacgctcg
     1261 acgatgcgat cgcagccttg aaggctccat ccgctggcaa tgcggccgcc agcgtggccc
     1321 tgagcaatgc cttgtccacc gccatccaga aaaccagtgt cgtttatgat aatgtgctgg
     1381 cggtgcgcgc atcggtggga tcgcgcatga atgaaatcga cgcattgaat gcgagtggga
     1441 cccagcgcga cgtgggctat tcaacacagt tgtctcagct tgaaagcgtt gactactatg
     1501 cggcatccac acagttgcaa ctgcgccaat cggcgctgga tgcggcgtca ttggcgttca
     1561 aaaaaataca gggcaacagc ctgttcaata tgggcaatgg cggatagata acgcctggtt
     1621 cattgccacg tttaactgtg ggcgccgcag gcgcgcgata ccgatcgctg gccttttgtt
     1681 tgcccaagca gcgcgcccag gcgtttcaat tcgggcgcgg ccaggttgcg aagtctggcg
     1741 tcatcgtcca gaatctgtgc cagcaaaggc tggcgcgccg cccgatcttc gctgcttaat
     1801 tgattcatgt ggcgcaatgc ctcgacagca agctggtatt gccgcgccag cgacgtcagg
     1861 ggatcccagt ggcttaaacg cgcctcggca aggatctggc tggacaggct ggcaataatt
     1921 tggtactgat tcaaaatcgg ggaagaagtg ctcatgcaag caggcgcaag aagtcgtgtt
     1981 tgtgggttcg atgttttata ccgatgccat tgccgcgggc tctggcgcct ctatggcctg
     2041 ccaggcctct ttcagttcat tcagcaggcg gtgcacttct tgcagccctt ctggttcgtt
     2101 cttcatattt gccatcaaaa gccggcgggt catgtagtcg tatagcgcat ccaggttctt
     2161 ggcaatgtcg ccgccggctt ctttgtccag gctggcgcgc aggccgttat caataatcga
     2221 aatggcaaag gaaactgatt tccccttgtt ggcgatgtcg ccggtgttca tgcattgaag
     2281 cgctgtggca atagctagaa gcgcgccgtc aaacagcatc acgatcagct tgtgcgggct
     2341 ggctgtagcg gccccgattt caaggccgat ttgtgcatag gcgcgcacgc cgctttgttt
     2401 cgatctatac attgtttctt cctcttcaat tattttggcg tg
//
LOCUS       DATCXU010000395         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_19973,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000395 DATCXU010000000
VERSION     DATCXU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19973"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="secD"
                     /locus_tag="VIR04_08830"
     CDS             <1..1226
                     /gene="secD"
                     /locus_tag="VIR04_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY9460891.1"
                     /translation="AITAVQSNAIKQNIQTLHNRINELGVAEPIIQQQGADRIVVQLP
                     GVQDVAKAKEILGRTATLEIRMVDDSPTALAALRAGTVPFGLERYTDRDGRPLLLRRQ
                     VILTGENLQDAQPGRDQQTQQPSVNLTLDAKGARIFRDVTRNNINKRMAIVLIENGKG
                     QVVTAPVIRSEIPGGQVQITGSMSAQEAADTSLLLRAGALAAPMKIIEERTIGPSLGA
                     DNITKGFHSTLFGFLAIAIFIIMYYHVFGLFSTTGLAFNVLLLLAVLSMLQATLTLPG
                     IAAIALTLGMAIDSNVLINERIREELRAGASPQQAIHLGFERAWATIVDSNLTTLIVG
                     LSLLAFGTGPIRGFAVVHCIGILTSMFSSVIGVRALANLWYGRRRKLARISIGEIWIP
                     KKEEHTPTRHAGAHR"
     gene            1252..2187
                     /gene="secF"
                     /locus_tag="VIR04_08835"
     CDS             1252..2187
                     /gene="secF"
                     /locus_tag="VIR04_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY9460892.1"
                     /translation="MEFFRIHRTIHFMRHALALNMVSLVTFLAAVFFIVTMGFHLSIE
                     FTGGTVMEVHYEKTAQVDDIRKAVDTLGYTDFQVTNFGTSRDVMIRLPIREGLNSAEQ
                     SKSVLNALKVQDPNVELRRVEFVGPQVGQELVHNGLMALLCVVIGIMLYLAMRFEWKF
                     AIAGVVANLHDVVIILGFFAFFQWEFSLSVLAGVLAVLGYSVNESVIIMDRIRENFRK
                     QRKSSPREIINSAITQTISRTIITHGSTQMMVMSMLFFGGPSLHYFALALTIGIWFGI
                     YSSVFVAAAITLWLGVKREDLVKPLKKDDPRAEIS"
BASE COUNT          500 a          653 c          591 g          495 t
ORIGIN      
        1 cagccataac agcggtccaa agcaacgcca taaaacaaaa catccagacc ctgcacaacc
       61 gcattaacga actcggcgta gccgaaccca tcatccagca acaaggcgcc gatcgcattg
      121 tcgttcagtt gccgggcgtc caggatgtcg caaaggcgaa agaaatcctc ggccggacgg
      181 ccacgctcga aattcgcatg gtcgatgatt cccccaccgc gctggcggcg ttgcgcgccg
      241 gcacggttcc cttcggactc gaacgttaca ccgatcgcga cggccgcccg cttttgttgc
      301 ggcgccaggt cattctaacg ggtgaaaacc tgcaagacgc ccaaccgggg cgcgatcagc
      361 aaacccagca gccttcggtc aatctgactc tggacgccaa aggcgcgcgc atcttccgcg
      421 atgtcacgcg caacaacatc aacaagcgca tggccatcgt gctgatcgaa aatggcaaag
      481 gtcaggtcgt caccgcgccg gtgatccgta gcgaaatacc tggcggccag gtacagatta
      541 caggcagcat gtccgctcag gaagcagccg acacgtcatt gctgctgcgc gccggtgcct
      601 tggcggcgcc catgaaaatt atcgaagaac gcacgattgg accaagcctt ggggccgaca
      661 acattaccaa ggggttccat tcgaccctgt ttggctttct tgcgattgcg atcttcatca
      721 ttatgtacta ccacgtgttc ggcctgttct cgaccaccgg actggctttc aatgtcttgc
      781 ttcttctggc cgtgctatcc atgctgcaag ccacattgac gctgccgggc attgccgcta
      841 tcgcgcttac gctaggtatg gctatcgact cgaatgtgct catcaacgaa cgcatacgcg
      901 aagaactgag ggcgggcgca tccccgcagc aagccattca tcttggtttc gaacgggcat
      961 gggccaccat tgtcgactcg aacctgacca ctctcatcgt cgggctgtcg ttgctggctt
     1021 tcggcacggg gcccatacgc ggctttgccg tggtgcactg catcggaatt ttgacatcca
     1081 tgttttcgtc ggtgattggc gtcagagcgc tggccaatct ctggtacggg cgccggcgca
     1141 agctcgcgcg catctcaatt ggcgaaatct ggataccaaa aaaagaagaa cacacaccga
     1201 cacgacatgc tggcgcacat cgctagactc ataacttagg caaagcgcaa catggaattt
     1261 ttccgcatcc accgcaccat acactttatg cgtcacgcgt tggcgctgaa tatggtcagc
     1321 ctggtgacct tcctggccgc ggttttcttt atcgtcacga tgggcttcca tctgtcgatc
     1381 gagttcaccg gcggcacggt catggaagtg cactacgaga aaacggcgca ggtcgacgat
     1441 atccgaaagg cggtcgacac gcttggctac accgattttc aggtaaccaa cttcggcacg
     1501 tcgcgcgatg tcatgatccg gctgcccatt cgcgaaggcc tgaactccgc cgaacaaagc
     1561 aaatcggtgc tgaatgcact gaaagtgcaa gacccaaacg tcgaattgcg gcgggttgaa
     1621 ttcgttggtc cgcaagtggg acaggaactg gtgcacaacg gcctgatggc cctgctatgc
     1681 gtggtcatcg gcattatgct ttatctggcc atgcggttcg aatggaagtt cgccatagcc
     1741 ggggtggtcg ccaacttgca cgacgtggtg attattctgg gttttttcgc cttcttccaa
     1801 tgggaatttt cactttcggt tctggcaggc gtgctggccg tgctgggata ctcggtcaac
     1861 gaatccgtaa tcatcatgga ccgaattcgc gaaaatttcc gcaagcaacg taagtcgtca
     1921 ccacgcgaaa tcattaacag cgctatcact caaaccattt cccgaaccat tattacgcac
     1981 gggtcgacgc aaatgatggt gatgtccatg ctgtttttcg gcggcccaag tctgcattat
     2041 tttgctctcg ctcttactat cgggatctgg ttcggtatct attcttctgt gttcgtggcc
     2101 gctgccatta cattgtggct gggcgtcaaa cgcgaggacc tggtgaagcc cttgaaaaaa
     2161 gatgatcccc gggcagagat cagctaaatt acattccgtt accgcaatcg ccatgcaaga
     2221 aatccccagc cgcatcgaa
//
LOCUS       DATCXU010000396         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_723362,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000396 DATCXU010000000
VERSION     DATCXU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723362"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VIR04_08840"
     CDS             complement(<1..494)
                     /locus_tag="VIR04_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9460893.1"
                     /translation="MRNTEFSGKIIWVVGATGALGTEISRRLAESGATVVASSRSRDK
                     LEALQSALESHSKIDIAPFDVTHDKEVKSVASEIISRHTHIDALINSTSVSLFADFLD
                     LDDETWHGVIESKLMAYVRTCRAVIPHMVKRGAGNIINISGRSARQPLPTHLPGGCVN
                     AAVN"
     gene            complement(510..1496)
                     /locus_tag="VIR04_08845"
     CDS             complement(510..1496)
                     /locus_tag="VIR04_08845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9460894.1"
                     /translation="MTFVQRIKIISSQLLLTMAASLVCCSAIAQEYPAKPITIVVGAS
                     AGGTTDIVARVIANELGKRLKQTVIVQNRAGAGGNIALGAVARAAPDGYTLGMAYSGL
                     AINPSVMNNMPFDTLKDLAPVSLVATVDMVLLTNPALGIRSVQDLIDAAKKQPERYAL
                     AANALGSVSHLSAASFKLRTGVEIPIVVYKGSAPALVDMIGGSVSAMFDTIPGALSYI
                     QQGQLNAIAVGATHRLPTMPNVPTLEEAGLKDFKIGSWYGVVAPAMTPAPVVNMLSGA
                     IAEILSDPEIKKRLAAQLLNLKGTSPAEFKSFLISEMKRWEEIAKSAKITIG"
     gene            1683..2507
                     /locus_tag="VIR04_08850"
     CDS             1683..2507
                     /locus_tag="VIR04_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY9460895.1"
                     /translation="MRQSGRATSVPNGDHEFEDDRQFATTLARGLDLLRCFSPFDPEL
                     RNKELSVRSGLPKATVSRLTYTLTRLGYLKQDPVSHKYRLGSAVLSIGYPLLANLDIR
                     QVARPYMRELAKYAKGWVSIGIRDRLNMIYIDSERSNDVWRVKPDVGQSFPILVSSMG
                     RAYLATLKERDRISLINEIKIKTPDLWQKYEAEIHRAMQAHGTLRFCTRHSDFVPDAH
                     SVGACMSQTGSDEIIAFNCAVPIYALQPNQLERDIGPELVRMVRKIEVAIRASKSP"
     gene            complement(2534..3706)
                     /locus_tag="VIR04_08855"
     CDS             complement(2534..3706)
                     /locus_tag="VIR04_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEY9460896.1"
                     /translation="MMDRRFRGAAAIAGVGHAGIGFATGFSEMEILVQAAHQAVADAG
                     LTMQDIDGIATASVTATMWVMPVIEHLGIRPTFIDSTMLGGSSFVAHLMPAVYALESG
                     QCSAVLVCYGSTQRTSSLSRAEIGALRRKLDPQPYETPYNPLNPLGSYALAAARHMHE
                     YGTTREQLAEIAVAARKWARLNPEALMHDPLSIEEVLSARMISDPLTVRDCCLVNDGA
                     GAYVLVRADRAKSLKQRPVYVLGNSTAVWNRQVSSMENLTITAARQSGDLAFSMAGVK
                     PADIDVVEVYDAFTINTLLFLEDLGFCKKGEGGDFVDEGAIAPGGRLPVNTNGGGLSC
                     AHPGMYGIFILIEAVRQLRGTCGARQVDGAELALVHGNGGTLSSQSTAILGTEATL"
     gene            complement(3712..4044)
                     /locus_tag="VIR04_08860"
     CDS             complement(3712..4044)
                     /locus_tag="VIR04_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY9460897.1"
                     /translation="MDQGNFVIQRCTSCRHSVFYPRMICPHCGSDQLEWFMPSGLGTV
                     YSTTIVRRKAEDGGDYNVALIDLKEGVRMMSRVEGISPEAVAIGMSVQARIIDDQGGK
                     LVVFHLAE"
BASE COUNT          935 a         1106 c         1057 g          955 t
ORIGIN      
        1 aaattcacgg ctgcgttgac gcagccgccc ggtaagtgcg tgggtaaagg ttgccgtgcg
       61 ctccggcccg agatattgat gatgtttccc gcacctcgct tgaccatgtg gggaattacc
      121 gcacgacacg ttcttacata ggccatgagt ttgctttcga taaccccatg ccaagtttcg
      181 tcgtcaagat cgagaaagtc cgcgaagaga gaaaccgacg tggagttgat taacgcgtcg
      241 atgtgcgtgt gtcttgaaat tatctcggat gcgacagact tgacctcttt gtcatgcgtg
      301 acgtcgaagg gcgcgatatc aattttcgaa tgggattcga gcgccgattg cagcgcctca
      361 agcttgtccc gggacctgct tgatgcgact accgtggcgc ccgactccgc aaggcgtcga
      421 gatatttccg tgcctagcgc tccagttgct ccaaccaccc aaataatttt cccggagaat
      481 tcagtatttc gcatgtttaa catagttgat catccgatcg ttatctttgc gcttttcgct
      541 atttcttccc atcgcttcat ttcagatatg aggaacgatt taaattcggc tggcgatgtg
      601 cctttgaggt tcagcaactg ggccgccagt cgttttttga tttccggatc ggaaagaatt
      661 tcagcaatag ccccgcttag catattgacc acgggagccg gcgtcattgc gggagccacc
      721 acgccatacc aagagccgat tttgaaatct ttcaggcccg cttcttccag cgttggcaca
      781 ttcggcattg taggtagccg atgcgttgcg cctacggcga tagcgttcag ttggccctgc
      841 tgtatgtacg acaaggctcc gggaatggta tcgaacattg cggaaacgct tcccccgatc
      901 atatcaacca gggccggcgc acttccttta tacacgacga tgggtatctc cacacctgtg
      961 cggagtttga acgatgccgc cgaaagatga gacaccgacc ccagcgcatt cgcagcaagc
     1021 gcatatcgtt cgggttgctt ttttgccgcg tctatcaggt cttggactga acgaatgcct
     1081 agtgccgggt tggtcagcaa aaccatgtcg actgttgcga ccaaagatac tggcgcgagg
     1141 tctttcaatg tgtcgaatgg catgttgttc attacggatg gattgatggc caaaccggaa
     1201 tatgccatgc ccagggtata cccatcgggc gccgctctgg cgaccgcgcc aagagcaatg
     1261 ttgcccccgg ctcccgctcg attctgaaca atgacagtct gtttcagtct ttttccgagc
     1321 tcgtttgcta tgactcgcgc gacgatatca gttgtgccgc cggccgaagc gcccacgact
     1381 attgttatcg gtttggctgg atattcctgc gcaatagcgg aacaacagac aagactggcc
     1441 gccatagtta ataacagttg cgacgaaatt atctttatcc tttgcacaaa ggtcattttt
     1501 ctgtctcctt accttgtcga attcttgttc aagaatgggt gattctaagt atcttgatcc
     1561 cggccgggtg ggaagtggct cactatgcga aatcaatacc tcgttctctt ccgttaaggt
     1621 atgctggcga tcattggcgt agacacgaca atatgttgaa ctgtgatcaa aagaggaatt
     1681 gaatgagaca gtccggccgt gccacatcag tgcccaatgg cgatcacgag ttcgaagatg
     1741 atcgtcaatt cgcgactaca ttggcccgcg ggctggattt gctgcggtgc ttttcgccct
     1801 tcgaccccga acttcgcaac aaagagcttt cggtccggag cggcctgcca aaggcgacag
     1861 tatccaggct tacctatact ttgactcgct tgggctacct gaaacaagac ccggtgtccc
     1921 acaagtaccg tttgggatcc gctgttcttt ccattggata tcccttgctg gccaacctcg
     1981 atattcgcca agtggcaaga ccatacatgc gggaattggc caagtacgcc aaggggtggg
     2041 tgtctatcgg catcagggat cggcttaaca tgatttatat agactctgaa cgctcgaatg
     2101 acgtttggcg tgtaaagcct gatgttggcc aaagctttcc gatcttggtc tcgtctatgg
     2161 ggcgagccta tctggcaaca ctgaaagagc gtgacaggat ctcgctgatc aacgaaatca
     2221 aaatcaagac gccggaccta tggcaaaaat atgaggcgga aattcataga gcgatgcagg
     2281 cacacggaac gctgcgtttc tgcactcgac acagtgattt tgttccggat gcgcattcgg
     2341 ttggggcatg catgtcgcag accggtagtg atgaaatcat cgccttcaac tgtgcggtgc
     2401 ctatttatgc ccttcagcct aaccagctcg aacgagacat aggaccagaa cttgtacgaa
     2461 tggtgcgcaa gatagaggtc gccataaggg cgtccaaatc gccatagtgg atttcagctt
     2521 taagggaaag cagctaaagc gtggcttcgg ttccaagaat ggcggtggac tgactggaca
     2581 gcgtgccgcc attgccgtgc accaacgcca attctgcgcc atctacctgg cgggctccgc
     2641 atgttcctcg cagttgtcgc accgcttcaa taagaataaa gatgccatac atgccagggt
     2701 gtgcgcatga caggccgccg ccattggtgt tgacaggtag acgaccgcct ggggcaatag
     2761 cgccttcatc cacaaaatcg ccaccttcgc ctttcttgca gaaacccagg tcttcaagga
     2821 aaagcagcgt gttgatggta aaggcgtcgt acacttcgac gacatcaata tcggccggct
     2881 taacccctgc catggagaac gccaggtcgc cagactgccg ggcggcagta atagtaagat
     2941 tttccatcga agaaacctgg cggttccaca cagcagtgga attacccaac acgtataccg
     3001 gcctttgctt gagcgacttg gcgcgatcag cccgaaccag cacataagcg ccagccccgt
     3061 cgttgacgag acagcagtca cgcacagtaa gaggatctga aatcatgcgg gcggacaata
     3121 cttcctcaat ggaaagcgga tcgtgcatca gcgcttccgg atttaatcgt gcccatttgc
     3181 gcgcagcaac ggcaatttct gctaattgtt cccgggttgt gccgtattca tgcatatggc
     3241 gcgccgccgc caaggcataa gagccgagcg gattcagtgg gttgtatggc gtttcatagg
     3301 gctgcgggtc caatttgcga cgcaacgcgc caatctcggc ccggctaagc gaggatgtgc
     3361 gctgcgtact gccatagcaa accagtaccg cgctgcactg cccggactcc aacgcataaa
     3421 cggctggcat caaatgcgcc acaaagctgg aaccgcccaa cattgtgctg tcaataaagg
     3481 tgggtctaat tcccaaatgc tcgataaccg gcatgaccca catggtggct gtgacgctgg
     3541 cagtggcaat accatcgatg tcttgcatgg taagcccagc atcagcgacg gcttggtgag
     3601 cagcctgaac caggatttcc atctcgctga atcctgtcgc aaagcctata cccgcatgcc
     3661 ccacaccggc gatagccgca gcgcctctga atcgtcggtc catcattatt tctactccgc
     3721 caagtggaag accactagct tgccgccctg atcatcaatg atcctggcct gaactgacat
     3781 tccaatcgcg actgcctccg gcgatatacc ttcgacccgg ctcatcatgc gcacgccctc
     3841 tttcaagtcg atcagcgcca cgttgtaatc gccaccgtct tcggctttgc gtcgcactat
     3901 ggtggtcgaa taaaccgttc ccaatccgga tggcatgaac cattccaatt gatcggagcc
     3961 gcaatgagga cagatcatgc gcggatagaa aacgctgtgc cgacacgagg tacaccgttg
     4021 aattacgaag ttaccctgat ccaattgtgc gcg
//
LOCUS       DATCXU010000397         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1275, whole
            genome shotgun sequence.
ACCESSION   DATCXU010000397 DATCXU010000000
VERSION     DATCXU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VIR04_08865"
     CDS             complement(<1..246)
                     /locus_tag="VIR04_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY9460898.1"
                     /translation="MNQISTQALTSWLDHTLQPQRFNDYCPNGLQVEGKPLISTIITG
                     VTASQALLEAAIKQNADAILVHHGWFWKNEDPRVRGPK"
     gene            272..1414
                     /locus_tag="VIR04_08870"
     CDS             272..1414
                     /locus_tag="VIR04_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY9460899.1"
                     /translation="MRRLWMLFAQTVTVCLGILFIVTTLRPDWLRSSPQSAAVRTTSA
                     PAMAEVSYAKAVAAAVPSVVNVYTSKQVRVPVVPLPNDPELNRLFRDIPGFSRRKQST
                     NLGSGVIVRADGYILTNYHVIESADAIEVALSDGRQARAALIGADPESDLAILKIDLP
                     KLSAITFNSDHQIRVGDVVLAIGNPFGVGQTTTMGIVSALGRNRLGVNIYENFIQTDA
                     AINPGNSGGALIDTTGHLIGINTAIYSETGGSLGIGFAIPARAAHTIMEQIIATGSVT
                     RGWLGIEPQDITPDLARAFNLRTDDGVIIARVLKDGPAWRAGVRVGDIVLKLNGQTVH
                     DTIEFLNQIAPLPPKQQVTLTIARSGKTQTITVEVGRRPPIRRPAS"
     gene            complement(1428..2228)
                     /gene="tatC"
                     /locus_tag="VIR04_08875"
     CDS             complement(1428..2228)
                     /gene="tatC"
                     /locus_tag="VIR04_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY9460900.1"
                     /translation="MTQDASADVNSQDTFISHLIELRTRLLRAVSAVLVIFVVLFLYP
                     GASKIYDLLAQPMLAALPAGTRMIATGVITPFMVPLKVTLLAAFVLALPVVLYEAWAF
                     VAPGLYKHEKRLAIPLILSSTFLFLLGMAFCYFVVFRTVFHFIANFAPQSITPAPDIE
                     AYLNFVMTMFVAFGVTFEVPIVVILLVKSGVVTVEKLRSVRGYVIVGAFVMAAVVTPP
                     DVLSQFMLALPICVLFEMGVLVASMLKKREVPAQDEADPSREVAPPAD"
     gene            complement(2225..2677)
                     /gene="tatB"
                     /locus_tag="VIR04_08880"
     CDS             complement(2225..2677)
                     /gene="tatB"
                     /locus_tag="VIR04_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY9460901.1"
                     /translation="MFNVSFSELLMIGIIALIVIGPERLPKVARTIGHLVGRAQRYVS
                     DVKSDIQREMDMDDIGSLKGQLEDAARSIRETVQNTAEDRRKPMDEAQAALKDAGETL
                     KETSESLKASVANTGPAAEKPAEPATEALAAPPGEPEQAIKPPAGTTT"
     gene            complement(2728..2964)
                     /gene="tatA"
                     /locus_tag="VIR04_08885"
     CDS             complement(2728..2964)
                     /gene="tatA"
                     /locus_tag="VIR04_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY9460902.1"
                     /translation="MGSFSIWHWLIVLVIVALIFGTKKLRNMGEDLGGAVKGFKKGMK
                     DASEESNTHAEAVTQERVPNGETIDVQAKEKSDT"
     gene            complement(2979..3347)
                     /locus_tag="VIR04_08890"
     CDS             complement(2979..3347)
                     /locus_tag="VIR04_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY9460903.1"
                     /translation="MDEHCLFCKIASGQIPSKKAYEDDDFFVFHDINPAAPVHLLLIP
                     KRHIVSLEGIQDSDFDWLGRMMVLAPKLALENGCRPGPGGGFRIVINNGTDGGQEIAH
                     LHCHIIGGPRPWDKRAAPAA"
     gene            complement(3366..3722)
                     /locus_tag="VIR04_08895"
     CDS             complement(3366..3722)
                     /locus_tag="VIR04_08895"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY9460904.1"
                     /translation="MNQTNHGPASNVLVRLADILESRLPENGGDPSSSYSAKLLAHGP
                     DAFLKKIGEEATELVMAAKDGVPNRIVSEAADLWFHCLVTLAYYKLRPEDVLLELERR
                     EGTSGLVEKASRKKST"
     gene            complement(3719..4123)
                     /gene="hisI"
                     /locus_tag="VIR04_08900"
     CDS             complement(3719..4123)
                     /gene="hisI"
                     /locus_tag="VIR04_08900"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY9460905.1"
                     /translation="MNQNDDWLAAVAFDENGLIPAIAQDGKSGAVLMMAWMNIEALTE
                     TAATGRAVYWSRSRQQLWRKGEQSGHIQIVHEMRLDCDGDVILLKVEQQEGIACHTGR
                     KSCFFRRLDGTFDKATWITVDPVLKDPKLIFK"
     gene            complement(4120..>4561)
                     /locus_tag="VIR04_08905"
     CDS             complement(4120..>4561)
                     /locus_tag="VIR04_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY9460906.1"
                     /translation="DYHGSQCIVVAIDARRVDARRTAEPGAAAQWEVFTHGGRKATGI
                     NVLEWAQKMAAYGAGELLLTSMDRDGTKAGFDLELTRAVSDAVPIPVIASGGVGTLQH
                     LADGVTTGRANAVLAASIFHYGQHTIKEAKEFMARQGIKVRLPS"
BASE COUNT          920 a         1269 c         1314 g         1058 t
ORIGIN      
        1 cttggggcca cgcacgcgag gatcttcgtt tttccagaac cagccgtgat gcaccaggat
       61 cgcgtcggcg ttttgtttga tcgctgcttc cagaagcgcc tgcgaggcgg taactccggt
      121 gataatggtg ctaatcagag gtttaccttc gacttgcagc ccgtttgggc agtagtcgtt
      181 aaagcgttgc ggttgcagcg tgtggtcgag ccagcttgtg agagcttgtg tggaaatttg
      241 attcaatttt tctctatggc cttttaatct tatgcgccga ttgtggatgt tgtttgccca
      301 gaccgttacg gtttgccttg gtattttatt tatcgtgacg acgctgcgtc ccgattggct
      361 gcgctcgtcg ccacaatcgg cggcggttcg aacaacgtct gcgcctgcga tggccgaggt
      421 gtcctatgcc aaagcggtcg ctgcggccgt tccgtctgtt gttaatgttt ataccagtaa
      481 gcaggtgcgc gtgccagtcg ttccgctgcc caacgacccc gaactgaatc gtctgtttcg
      541 agatattccc gggttcagcc gtcgcaagca gtccaccaat ctggggtctg gggtgatcgt
      601 tcgggccgat ggctatattc tgacgaatta tcatgtgatt gaatccgctg atgcgatcga
      661 ggtggccttg agcgacggcc gtcaggcgcg ggccgccctg atcggcgccg acccggaaag
      721 cgatcttgcc attttgaaga ttgatttgcc gaagttgtcg gcaattacct tcaatagcga
      781 ccatcagatt cgcgtcgggg atgtggttct ggccattggc aatccgttcg gagtgggtca
      841 aacaaccact atggggattg tttccgcgtt ggggcgcaat cgtctcggag tcaatattta
      901 cgaaaacttc atccagaccg atgcggcaat caatcccggc aattcaggtg gcgcattaat
      961 tgatacgaca ggtcatctta ttggaatcaa taccgccatt tattccgaaa ccggtggctc
     1021 gttgggcatc ggttttgcga ttcccgcccg cgcggcgcac accatcatgg agcaaattat
     1081 cgccaccggt tcggtaacgc gcggctggtt ggggatcgag cctcaggata ttacgcccga
     1141 cctggcccgg gcgtttaatt tgcgcacgga cgatggcgtc attattgcgc gggtgctcaa
     1201 agacgggccg gcgtggcgcg cgggggttcg cgttggcgat atcgtcctga agttaaacgg
     1261 gcagaccgtg cacgacacga tcgaattctt gaatcagatt gcgcccctgc cgcccaagca
     1321 acaggttacg ctgactattg cgcgctccgg taaaacgcag accataacgg tcgaagtcgg
     1381 ccggcgtccg cctatcagac gccccgcgtc gtagtcgccg ccgcggttca gtcagcgggc
     1441 ggcgcaacct cgcgggaagg gtctgcttcg tcttgtgccg ggacttcgcg ttttttcagc
     1501 atgctggcga ccaaaacccc catttcaaag agtacgcata tgggaagggc cagcatgaac
     1561 tggctaagta catcgggcgg ggtaacgaca gctgccatga cgaaagcccc cacaatcacg
     1621 tagccgcgta cagatcttag cttctcgaca gtgacgaccc cgcttttcac cagcaggatg
     1681 acaacaatcg ggacttcgaa cgttacgccg aacgcgacga acatggtcat gacaaagttc
     1741 agataggctt ctatgtcggg ggcgggcgtg atcgattgcg gcgcgaagtt ggcaataaag
     1801 tggaataccg tgcgaaacac gacaaaataa caaaacgcca tgcccagtaa aaacaggaac
     1861 gtgctggaaa ggattagcgg tatggccagg cgtttttcat gcttgtacag gccaggcgcc
     1921 acaaaggccc acgcctcgta cagcacgacc ggcaaggcca gcacaaacgc cgccaggagc
     1981 gtaactttca aaggcaccat gaacggcgta atgacgccgg tggcgatcat tcgtgttccc
     2041 gcgggcagcg cggcgagcat cggctgcgcc agcaaatcgt agattttgga ggcgccggga
     2101 taaagaaaca ggacgacaaa aataaccagc actgccgata cggcgcgcaa caaccgcgtg
     2161 cgcagttcga tcaggtgcga aataaaggtg tcttgcgaat taacgtcggc ggaggcatcc
     2221 tgggtcacgt cgtagtcccc gctggcggct tgattgcctg ttccggctcg cccgggggcg
     2281 cggccaaagc ctcggtcgcg ggttcggccg gcttctcggc cgcgggacca gtgttcgcga
     2341 ccgatgcctt aagcgattca ctggtttctt taagcgtttc gccggcgtct ttgagcgctg
     2401 cctgggcctc gtccatcggt ttgcgccgat cttcggccgt gttttgcacc gtttccctga
     2461 tggaccgggc ggcgtcctcg agttgcccct tcagactgcc gatgtcgtcc atgtccattt
     2521 cgcgctgaat gtcggatttc acgtcgctga catagcgttg cgcgcgtccc accagatggc
     2581 cgatggtgcg cgctaccttc ggcaaccgtt cagggccgat cacgatcagc gcaatgatgc
     2641 cgatcatgag cagttcgcta aagctgacat taaacattag ggatcaattc acataaaaag
     2701 aagcatggcg ggaacgaatc gcaaaagtca ggtgtcggat ttttctttag cttgcacatc
     2761 aatggtttcg ccgtttggaa cgcgttcctg cgtgacagct tcggcgtgtg tattggactc
     2821 ttcgctggcg tctttcatgc cttttttgaa gcctttgacg gcgccgccca agtcttcgcc
     2881 catgttgcgt agtttcttgg tgccaaaaat aagagccacg atgactaaaa cgattagcca
     2941 gtgccagata ctgaaactac ccatgatcgt attctccgtt atgcggcagg cgccgctctt
     3001 ttatcccagg ggcgcgggcc cccgatgatg tgacagtgca aatgggctat ttcctgccca
     3061 ccatctgttc cattgttgat gactattcga aagccgccgc cgggccccgg acggcaacca
     3121 ttttcaagcg ccagctttgg cgcaaggacc atcattctac ccaaccaatc gaaatcactg
     3181 tcctgaatgc cctccagcga cacgatatgg cgttttggaa taagcagcaa gtgtaccgga
     3241 gcggccggat ttatgtcgtg gaaaacaaag aaatcgtcat cttcgtaggc ctttttggaa
     3301 gggatctggc cactggcgat tttacagaat agacaatgct catccattat agttctcgga
     3361 gtaacttaag ttgatttctt gcggctggct ttttcgacca atcccgatgt tccttcgcgc
     3421 cgctccagtt cgagcaatac gtcttcgggg cgcagcttat agtaggccag agtgaccaga
     3481 caatgaaacc acaggtcggc cgcctcgctg acgatgcggt tgggaacacc gtctttggcg
     3541 gccatgacca gctccgtggc ctcttcgccg atttttttta aaaaagcgtc gggtccgtgc
     3601 gccagcaatt tggccgaata cgacgacgaa gggtcgccgc cattttcggg caggcgcgat
     3661 tcaagaatat cggccaggcg cacgagcacg ttgctggcgg ggccgtgatt ggtttggttc
     3721 atttgaaaat cagttttgga tctttaagaa caggatcgac ggtgatccag gtggctttgt
     3781 cgaacgtccc gtccagtcgc cggaaaaagc agcttttgcg ccccgtatgg caggcgatgc
     3841 cctcctgttg ctctactttg agcaagatga cgtcgccatc gcaatccagg cgcatttcat
     3901 gcacgatctg tatgtggccc gactgttcgc cttttcgcca caactgttga cgcgaacggg
     3961 accagtaaac ggcgcgcccg gtggcggccg tctcggtcag ggcttcgata ttcatccacg
     4021 ccatcatgag caccgccccg gattttccgt cctgcgcgat agcgggtatt agaccgtttt
     4081 catcaaaggc cacggccgct agccagtcat cattttggtt catgatggta atcgtacctt
     4141 aataccttgc ctggccatga attctttggc ctctttgatc gtgtgctgcc cataatggaa
     4201 gatgctggcg gccagcacgg cgttggcccg gcccgtggtt acgccgtcgg ccagatgctg
     4261 cagagtgccg accccgcccg acgcgatgac cggtattgga acggcgtcgg agacggcccg
     4321 cgtcagttcc agatcgaagc ccgctttcgt gccgtcgcgg tccatgctgg tcagcagcag
     4381 ttcgcccgcg ccgtaggcgg ccattttttg agcccactcg aggacattga tgccggtggc
     4441 cttgcggccc ccgtgagtga aaacttccca ttgggccgcc gccccgggtt cggctgtgcg
     4501 tcgggcgtcg acacgtcggg cgtcgatggc aaccacgatg cattgcgaac cgtgatagtc
     4561 g
//
LOCUS       DATCXU010000398         9423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_995161,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000398 DATCXU010000000
VERSION     DATCXU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995161"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2759
                     /locus_tag="VIR04_08910"
     CDS             <1..2759
                     /locus_tag="VIR04_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY9460907.1"
                     /translation="GRGQGVRLDANATVLPRAAFPFRQAVLGLELADGSSLNANIDWD
                     SQTAGDVIHDHLAGSVRSKKLNLGQLAGSVIPPAVISTSMNFDIRLLNRRALSAADLD
                     IEFSPDSRWNKQALSGVLKAKITDSSEGAAALGADSWKTLRLADITMDLHLGKNHLTA
                     NGALGDAGSQLNLNVLAPQLAAFWPGIAGGIKLQGRAGGSAAAHTADLTATYTPANSQ
                     PNDVAAAPIEIALRASGAWGGPVSAQTTGGVEPGWHGALSGLRVRRGGISATLEKSLA
                     LELLPGAAAPAWQWQIGAGAIKVSLPSQTEFVIRHKGSRGGPGRWETQGAIDRLTLSS
                     DVVAALRTEDGNGAQQVDSRGAKADGKKNRGTGQIVFDSDWDLTFAGTLEGQAHIKRS
                     SGDLRVPGDPGFMLGLQAMDVDVVARRAGASTSKVIANMNVVTAKMGRIAASGSALLH
                     VSPGGAFSFNKKDPKEVTVQADVADLGWASLFLGDATDLGGSLKANLKAQSQADGAWA
                     ASGTVTGQKIRLVRLDDGVRLLDGTLAAHLEGNRFVLDSLRFPAQQRVTPKEWRTNEW
                     LTTNPQAKGGSLTLSGAWNLFDSTGAINVDLVRYPILQRADRYAMVTGKLTVNAPLPK
                     IVIAGSVTADAGWFDLDVLGGVPVVDNDVIVIRPGEARKTEVPADISLDLTVNLGPRF
                     YLTGYGVNSGLLGSLRILTVDRRLTGEGELRTRGGSIDVYGQHLQLRRGTVTFQGDIA
                     NPVLNIEALRTGVSVQAGVRVAGTAKRPRIGLVSYPDVSDVEKLSWLLLGRAPDDSGG
                     DAALLFSVGTSLFGGGEPFYRKFGLDEVSMRSGALGSTGSILPVESVVSGLDSGTSDI
                     ERKFIVLAKTLSNGITLSLEQALSQTGTVARASYRLARGLNAEISAGTVNGIALVYRV
                     FSKR"
     gene            2838..3401
                     /gene="dcd"
                     /locus_tag="VIR04_08915"
     CDS             2838..3401
                     /gene="dcd"
                     /locus_tag="VIR04_08915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY9460908.1"
                     /translation="MAIKSDRWIRRLGGEGMIEPFEPGQVRTANGQKIVSYGTSSYGY
                     DVRCADEFKIFTNINSTIVDPKAFDERSFVDFRGDVCIIPPNSFALARTVEHFRIPRS
                     ILTVCLGKSTYARCGIIVNVTPLEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQMLFFE
                     SDEICEISYRDRGGKYQGQQGVTLPRA"
     gene            3415..5676
                     /locus_tag="VIR04_08920"
     CDS             3415..5676
                     /locus_tag="VIR04_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEY9460909.1"
                     /translation="MKFRFPIMIIDEDYRSENASGFGIRALADAIEAEGVDVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFDVDSPEDVANAIKNLRTFIGELRFRNADIPIYL
                     YGETRTSQHIPNDILRELHGFIHMFEDTPEFVARHIIREARTYLDGLAPPFFRELVKY
                     ALDGSYSWHCPGHSGGVAFLKSPVGRMFHQFFGENMLRADVCNAVDELGQLLDHTGPV
                     AESELNAARIFHADHCFFVTNGTSTSNKIVWHANVAAGDVVVVDRNCHKSILHAITMT
                     GAIPVFLRPTRNHLGIIGPIPLEEFEPENIRKKIEANPFARLAKNKRPRILTLTQSTY
                     DGIIYNVEMIKEKLGSEIDTLHFDEAWLPHAAFHEFYKDMHAIGDDRPRSKDAIVFAT
                     HSTHKLLAGLSQASQITVQDAENRKLDLGLFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESISEAMDFRRAMRKIESEFGKNDWWFKVWGPNRLASEGIGNRDDWIL
                     ESGDEWHGFGALAEGFNMLDPIKATIVTPGLDISGTFDDTGIPAALVTKFLAEHGVVV
                     EKTGLYSFFILFTIGITKGRWNTLLTALQQFKDDYDRNQPLWRILPEFCQQHRVYEKM
                     GLRDLCQQIHAAYRKHDVARLTTEMYLSDMVPAMRPADAFAKMAHREVERVAIDDLEG
                     RVTGVLLTPYPPGIPLLIPGERFNRVIVHYLQFAHEFNANFPGFETDIHGLAHDKISD
                     GTERYYVDCIREA"
     regulatory      5847..5933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      5955..6065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            6116..7213
                     /gene="gcvT"
                     /locus_tag="VIR04_08925"
     CDS             6116..7213
                     /gene="gcvT"
                     /locus_tag="VIR04_08925"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY9460910.1"
                     /translation="MSLALKRTPLYRIHTELGAKMVDFGGWEMPLAYGSQIEEHHAVR
                     RDAGMFDVSHMQSVDIKGSGSTALLRRLLANDVAKLSTPGKALYSCMLNPQGGVIDDL
                     IVYFFDSDTWRVVVNAATAEKDFAWMRRVASAEKFDVSITPRPDLAMVAVQGPDARHK
                     LWAARPAWQAATEPLTPFTGVFVADDTLVARTGYTGEDGFEVVLPATQVESLWNDLIK
                     QKVHPCGLGARDTLRLEAGMNLYGQDMDELVQPQESGLSWTVSLKDPQRRFIGRDAIE
                     SLATDRMLIGLRLLDRGVMRPHMKVRTAQGEGEMTSGTMSPTMGTSIGRARVPGGAKT
                     GDQVQVEIRGKWFAAEVTKMPFVRNGKVLAA"
     gene            7266..7643
                     /gene="gcvH"
                     /locus_tag="VIR04_08930"
     CDS             7266..7643
                     /gene="gcvH"
                     /locus_tag="VIR04_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY9460911.1"
                     /translation="MNLPNDRKYTPSHEWVKAEGDVFLVGITDNAQDQLGDLVFVGDV
                     NPGATLKAGETAGVIESVKAASDIYAPVDGEIVAFNDALESEPDLINKAAFDTWIFKI
                     KPNNAADVDALLDATAYQSQASV"
     gene            7653..>9423
                     /gene="gcvPA"
                     /locus_tag="VIR04_08935"
     CDS             7653..>9423
                     /gene="gcvPA"
                     /locus_tag="VIR04_08935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY9460912.1"
                     /translation="MLLELDPHADFIGRHIGPASADQKKMLSAMGVPDMQSLVRQVVP
                     AGILMKGGLGLPASRSEPDVLAEVKEIAGRNRVFRNYIGQGYYGTHVPNVILRNILEN
                     PAWYTAYTPYQPEISQGRLEALLNYQTMVADLTGLDIANSSLLDEGTAAAEAMTMARR
                     TSRAKSRVFFASRHCHPQTLQVLRTRADGLDIELVVGDESQNLPECFGVLLQYPHSLG
                     GIVDYRQLTEAAHARGAVVTVATDLLALALLTPPGEWGADIAVGSTQRFGVPLGFGGP
                     HAGFMACKDAFKRTMPGRLVGVSVDSQGDMAMRLALQTREQHIRREKATSNICTAQVL
                     LAVMAGMYAVWHGPSGIKRIAQRVARLTVCLRQSLQSLGITSVNDHFFDTLLLDTGIR
                     TDSIVQAAHAASINLRHVSSTQLAISLDETVTPADLTVLVGAFAKALNKTPVEPDWPG
                     LAMSTEQCIPAGLRRLSPILTHPVFSKIQSETDMLRYLRGLADKDLALDRTMIPLGSC
                     TMKLNATAEMIPVTWPEFGAMHPYAPADQTRGYQELIRRLSAALCEITGYDSISLQPN
                     SGAQGEYSGLLAIRGYHRANGQGQ"
BASE COUNT         1930 a         2527 c         2886 g         2080 t
ORIGIN      
        1 acgggcgcgg ccagggcgtg cgcctggatg ccaatgccac ggtgctgcct cgcgccgcct
       61 ttccgttcag gcaagcggtg ctgggcctcg agctggccga tggctcgtct ctgaatgcca
      121 acattgattg ggattcacaa acggccggcg atgtaatcca cgatcatctt gcgggctccg
      181 tgcgaagcaa gaaattgaat ctggggcaat tggccgggtc ggttattccc cccgcggtca
      241 tcagcacgtc gatgaatttt gacattcgat tgttgaatcg ccgggcgctt tccgccgccg
      301 atctggatat tgaatttagt cccgattcgc gctggaacaa gcaagctttg tccggagtat
      361 tgaaggcgaa aatcactgat agctcagagg gcgccgcggc gctcggggcg gattcgtgga
      421 aaacactgcg gctggccgat ataacgatgg atttgcatct cggcaagaat catttgaccg
      481 cgaatggcgc gctgggcgac gccggcagcc aactgaattt gaatgtactg gctccgcaac
      541 tggcggcttt ctggccgggc atcgccggcg gcataaagct gcaaggccgc gcaggtggct
      601 ccgcagcggc ccatacggcg gatttgacgg cgacttacac tcccgcaaac agtcagccca
      661 acgacgtcgc ggccgcgccc attgaaatcg cgttgcgcgc atcaggcgcc tggggcggcc
      721 ccgtttcagc gcagacgacg ggcggcgtgg agccgggctg gcatggcgct ttgtccgggc
      781 ttcgggttcg tcgcggaggc ataagcgcta ctttggaaaa atcgcttgcg ctcgagcttt
      841 tgcccggggc ggcggcccct gcctggcagt ggcaaattgg cgccggggcg atcaaagttt
      901 cgctgccttc gcaaaccgaa tttgtcatca gacacaaagg atcgcgcggc ggtccgggcc
      961 ggtgggaaac acaaggcgcc attgacagat tgacgctgtc ctcggacgtt gtcgcggccc
     1021 tgcggaccga ggacggtaac ggcgcgcaac aggtagatag ccgcggcgcc aaggcggacg
     1081 ggaaaaaaaa tcgcggaacc gggcagatcg tgtttgactc ggactgggat ctgacatttg
     1141 ccggcactct tgagggccag gctcacatta aacgcagttc gggcgatttg cgcgtgccag
     1201 gcgatcccgg ttttatgctg ggcctgcagg cgatggatgt tgatgtcgtc gctcggcgcg
     1261 ctggtgcgtc gacaagcaag gtcatcgcga acatgaacgt tgtcaccgcc aagatggggc
     1321 ggattgcggc aagcgggtcg gcgctgcttc atgtttcccc gggcggcgcc ttcagcttta
     1381 acaaaaaaga tccgaaagag gtaaccgtgc aggctgatgt tgcggatctg ggctgggcca
     1441 gcctgtttct gggcgacgcc actgacctgg gcggctcgct taaagcaaac ctgaaggcgc
     1501 aaagccaggc ggatggggcc tgggctgcca gcggaacggt gacggggcaa aaaatacggc
     1561 tggttcgtct tgatgatgga gtgcgccttc tggatgggac gctggcggcc caccttgagg
     1621 gcaaccgctt tgtattggac agcttgcgct tcccggcgca acaacgcgtc accccgaaag
     1681 aatggcgcac gaacgagtgg ctgacaacca acccgcaggc caagggcggc agcctgacgc
     1741 taagcggcgc ctggaatctg ttcgactcga ccggcgcaat caatgtggat ttggttcgct
     1801 atccgatttt gcagcgtgcg gatcgctacg ccatggtgac cggtaagttg acggtcaacg
     1861 cgccgcttcc gaaaattgtc atcgccggca gcgtaacggc cgatgctggc tggttcgatc
     1921 tggacgtgct gggcggcgtc ccggtggtcg ataatgacgt gattgtcatc aggccgggcg
     1981 aggccaggaa gacggaggtg ccggccgata tttccctgga tttaacggtc aatctcggac
     2041 cgcgcttcta tttgacgggt tatggcgtga actcgggatt gcttggcagc ttgcgcattc
     2101 ttacggtcga tcgccggttg accggcgaag gcgagctcag gacgcgcggg ggctcgattg
     2161 atgtctatgg ccagcatttg cagcttcgtc gcgggacagt gacgtttcag ggcgatatcg
     2221 ccaacccggt cctgaacatt gaagcgctgc gcactggggt ttcggtccag gcgggtgtgc
     2281 gggtggctgg caccgccaag cggcccagaa ttggattagt atcctacccc gatgtcagcg
     2341 acgtcgaaaa gctatcctgg ttgttgttgg gtcgcgcgcc cgatgacagc ggcggcgacg
     2401 cggcgcttct gttttcggtg ggcacttcgc tgtttggcgg cggtgagccg ttttatcgca
     2461 agttcggact tgacgaagtc agcatgcgtt cgggcgcttt aggcagcacc ggtagtatat
     2521 tgccggtcga gagcgtggtc agcggcctgg atagcggcac gagcgatatc gaacgcaaat
     2581 tcatcgtctt ggcgaaaacg ctgtccaacg gtattacttt aagccttgag caggcgctgt
     2641 cccaaacagg cacagtggcg cgggccagct accggttggc gagagggctg aacgctgaaa
     2701 tcagcgcggg cacggttaac gggattgcat tggtttaccg tgttttttcc aaaaggtagt
     2761 ttttgccgat ttgttgcaag gactcgtttt ttcgggtggg cgataaaata agcgcccccc
     2821 agacaaggtg acaagcaatg gctataaaaa gtgatcgatg gattcgacgc cttggcgggg
     2881 aaggcatgat agaacccttc gagcccgggc aggtgcgaac ggctaacggc cagaagatcg
     2941 tgagttacgg cacgagcagt tatggctatg acgtacgctg cgcggacgaa ttcaagatct
     3001 tcaccaatat caattcgacc attgtggatc cgaaggcatt cgatgaacgt tcttttgtcg
     3061 atttccgcgg cgatgtgtgc attattccgc ccaattcatt tgctttggcg cgcactgtcg
     3121 agcattttcg aattccacgt agcatactta cagtttgcct gggcaaaagc acgtatgccc
     3181 gctgcggcat tattgtcaac gtaacgccgc tcgagccgga atgggaaggc tatgtcaccc
     3241 ttgagttttc gaacacgacc cccttgcccg ccaagattta tgcgggcgag gggtgcgcgc
     3301 aaatgttatt ttttgaaagc gacgagatat gtgaaatctc gtatcgcgat cgtggcggca
     3361 agtatcaagg tcagcagggc gtgactctgc ctcgggccta ggggagttgt tccaatgaaa
     3421 ttccgttttc ccatcatgat tattgatgaa gactaccgtt ccgaaaatgc ttcgggtttc
     3481 ggtatacgcg cattggccga tgccattgaa gccgaagggg ttgacgtgct gggcgtcacc
     3541 agttacggcg acctgagttc atttgctcag caacaaagca gagcgagcgc attcatcttg
     3601 tcgatcgatg atgaggaatt cgacgttgac tcgcccgaag acgtcgccaa tgccatcaag
     3661 aatttgcgca cgttcattgg ggagctgcgt tttcggaatg cggacattcc catttacctt
     3721 tatggcgaga cacgcacctc gcagcatatt ccgaacgaca tattgcgaga actgcatggc
     3781 ttcattcaca tgtttgaaga cacgccagag tttgtggcgc gccacatcat ccgcgaagcg
     3841 cgcacctacc ttgatggcct ggcgccaccg tttttccggg agctggtcaa atacgccctg
     3901 gacggttcct attcatggca ctgccccggt cattcgggcg gcgtggcgtt cttgaagagt
     3961 ccggtaggac gaatgtttca tcagtttttt ggtgaaaaca tgttgcgcgc cgatgtatgc
     4021 aatgcggtcg atgagctggg tcagttgctg gatcacacgg gtcctgtagc cgaatcggaa
     4081 ctgaatgcgg cgcgcatctt tcatgcggac cattgctttt tcgtgaccaa cggcacctcg
     4141 acttcgaaca aaatagtatg gcatgccaac gtggcggcgg gcgacgttgt ggtggtcgac
     4201 cgtaactgtc acaagtcaat tttgcacgcc attaccatga caggagccat ccctgtgttc
     4261 ttgcgcccca cgcgcaacca cctgggaatc attggtccga ttccgctcga ggaattcgag
     4321 cccgaaaata tccgcaaaaa gatcgaggcc aatccgtttg ccaggctggc caaaaacaag
     4381 cggccgcgta tcctgacatt gacgcaaagc acctacgacg gaatcatcta taacgttgag
     4441 atgatcaagg aaaagctggg ctcggaaatc gacaccctgc atttcgacga ggcctggttg
     4501 ccgcatgcgg cctttcacga attttataaa gacatgcacg ccatcggaga cgaccggccg
     4561 cgcagcaaag acgccatcgt gtttgccacg cactcgactc ataaactatt ggccggcctg
     4621 tcgcaggcct cgcaaattac ggtgcaggac gcggaaaacc gcaaacttga tctgggcctg
     4681 tttaacgagg cctatcttat gcatacctcg acatcgccgc agtacgccat tattgcgtct
     4741 tgcgacgtgg ctgccgccat gatggagcct ccgggcggaa cggcgctggt cgaggaaagc
     4801 attagcgaag ccatggattt tcgtcgggcc atgcgcaaaa ttgaatcgga gttcggaaag
     4861 aacgactggt ggttcaaggt ttgggggcca aaccggttgg ccagcgaagg catcggcaat
     4921 cgcgacgact ggatactcga atcgggcgac gaatggcacg gctttggcgc cctggccgag
     4981 ggcttcaata tgctggatcc gatcaaggcg accattgtta ccccgggttt ggacatttcg
     5041 ggcacgtttg acgatacggg cattccggcg gcgctggtca caaaatttct ggcggaacat
     5101 ggtgtggtgg tcgaaaagac cggcctgtat tcgttcttta tcctattcac catcggtatt
     5161 accaaggggc gctggaatac tcttttgacg gcattgcagc agttcaaaga cgattacgat
     5221 cgcaaccagc cgctatggcg cattttgccg gaattctgtc agcaacatcg tgtgtacgag
     5281 aaaatgggct tgcgcgattt atgccagcag attcatgccg cttatcgcaa acatgatgtg
     5341 gcgcgcctga ctaccgagat gtatctgagc gacatggtcc cggccatgag gccggcggac
     5401 gcgttcgcca aaatggcgca ccgtgaagtt gaacgcgtcg cgattgatga cctggaaggg
     5461 cgtgtgacag gggtattgct cacgccctat cctcctggca ttcctctctt gattccgggc
     5521 gaacgtttta atcgggttat cgttcattac ctgcagttcg ctcacgagtt caacgcgaat
     5581 ttcccgggtt ttgaaacgga tattcatggt cttgctcatg acaagatctc cgacggcacg
     5641 gaacgctatt acgtcgattg catccgggaa gcgtaaggct gcaattctgg cggcgcgctt
     5701 cggcagcgga aacacagcgg acacacgctt ggcgcggtgt tgcaatggcg cgccagttgt
     5761 cggcgttgcc ggcgtatatc tggggttatt tcggctaatg tatttggtgt tcgcctgctt
     5821 tcgggttatg ctttgcgctg ctgtcgttac gtagagtagg agagtgccgg tttccggccg
     5881 ccgaaggcgc aattcgccca gaatcgctca ggcccccgta ctgctcacgt attgtcccga
     5941 tgcaagccgg ggcaggatgg ctatctggag agactcgcgt cgtgtgcaaa tgtcacgttt
     6001 cgggcgccga aggtgaatac ccgccatgcg ggccaactct caggcaaaag gacagaaggg
     6061 cggtatcagc cggtttatag ttggtttccg tccttttctg tttttcggag tctccatgtc
     6121 tttagcgtta aagcgcactc ccctataccg tattcatact gaactgggtg ccaaaatggt
     6181 cgattttggc ggctgggaaa tgccattggc ttacgggtct caaatcgaag aacatcacgc
     6241 ggtacgccgc gacgcgggca tgttcgacgt ctcgcacatg caaagcgtcg atatcaaagg
     6301 aagcggctcc acagcgcttc tgcggcgctt gctcgccaac gacgtcgcca aactttcgac
     6361 gccgggcaag gcgctatatt cctgcatgct caatccacag ggtggcgtca tcgacgatct
     6421 gatcgtctac ttcttcgaca gcgacacttg gcgcgtggtg gtcaatgcgg ccacggccga
     6481 aaaggatttc gcctggatgc ggcgggtggc cagcgccgaa aaatttgatg tgtcgatcac
     6541 cccacgcccc gatttggcca tggtggctgt gcaagggcct gatgcgcgcc acaagctttg
     6601 ggcggcgcgc cccgcctggc aagcggccac cgaaccgcta acgcctttta caggtgtatt
     6661 cgttgccgac gacactttgg tggcgcgcac gggatacaca ggtgaagacg gtttcgaagt
     6721 cgtcttgccg gcgacgcagg tcgaatcatt atggaacgat ctgatcaaac agaaagtgca
     6781 tccctgcggc cttggcgccc gcgatacgct gcgcctggaa gcgggcatga atctgtatgg
     6841 gcaagacatg gatgaactgg ttcaaccgca agagtccgga ctttcatgga ccgtgtcatt
     6901 gaaagatccg caacgccggt tcattggccg tgacgcgatt gagtcgcttg ctacggatcg
     6961 tatgctgatt ggattgcggc tgcttgaccg cggcgtaatg cgcccgcaca tgaaggtgcg
     7021 caccgcgcaa ggcgagggcg aaatgaccag cggcacgatg tctccgacca tgggaacgtc
     7081 cattggcagg gcgcgagtgc ctggcggcgc caaaacgggc gatcaggtcc aggtcgagat
     7141 tcgcggcaag tggttcgccg ctgaagtcac gaagatgcct ttcgtgcgca acggcaaagt
     7201 acttgctgcc tagttccgtg tttgcttcaa accggtgaaa cgaacaattt ttaacgaggt
     7261 gactcatgaa tctacctaat gatcgcaagt acacaccgtc gcacgaatgg gttaaagccg
     7321 aaggcgacgt atttttagtg ggcattaccg acaacgcgca agatcagttg ggcgacctgg
     7381 tttttgtggg cgacgtgaat ccgggcgcaa cgctgaaagc gggcgagacc gccggggtga
     7441 ttgaatcggt taaagcggcg tccgacattt acgcgcccgt ggacggcgaa attgtcgcat
     7501 ttaatgacgc gctggaatcg gagcccgatc tgattaacaa ggcggctttc gatacctgga
     7561 ttttcaagat aaaaccgaat aacgcggctg atgtcgacgc gttgctggat gcgacggcgt
     7621 atcaatcgca ggcttctgtt tagggcgtaa tcatgttgct tgaattagat ccgcacgccg
     7681 actttattgg gcgccacatc gggccggcat cggccgatca gaaaaagatg ttgtccgcga
     7741 tgggcgtgcc cgacatgcaa agcctggtgc ggcaagttgt tccggccggt atcctgatga
     7801 aaggcgggct gggcttgccc gcttcgcgta gcgagcccga cgtcctggcc gaggtcaaag
     7861 agatcgcggg ccgcaatcgg gtgtttcgca actatattgg tcagggttat tacggcactc
     7921 acgtgccgaa cgtaatcttg cgcaatattc tcgagaaccc tgcgtggtac acggcctaca
     7981 cgccgtatca gcccgagatt tctcagggcc gtctcgaggc gcttttaaat tatcagacaa
     8041 tggtggccga cctgactggt ctggacattg ccaactcgtc gctccttgac gagggcacgg
     8101 ccgccgccga agccatgacg atggcgcgcc ggacttcgcg cgcgaaaagc cgggtatttt
     8161 ttgcttccag gcattgccat cctcagactc ttcaggtttt gcgcactcgt gccgacggcc
     8221 tggatatcga gctggtggtg ggcgatgaat cgcaaaatct gccggaatgc tttggcgtcc
     8281 ttttacagta tccgcacagt ttgggcggaa tcgtggatta tcggcaactg accgaggccg
     8341 cgcacgctcg tggcgcagtg gtcaccgttg ccaccgatct gctggcgctg gctttgttga
     8401 cgccgccggg ggaatggggg gccgatatcg ctgttggctc gacgcagcgt tttggcgtgc
     8461 ctctgggatt tggcggcccg cacgccggct tcatggcgtg caaagatgca ttcaagcgca
     8521 cgatgcctgg ccgcctggtg ggcgtgtcgg tggatagcca gggggacatg gccatgcgcc
     8581 ttgccctgca aacgcgcgag cagcacatac gccgcgagaa agccacctcg aatatttgca
     8641 cggctcaggt attgcttgcc gtgatggctg gcatgtacgc ggtatggcat ggcccatcgg
     8701 gcatcaaacg catagcacaa cgcgttgcac ggctgacggt ctgcctgagg caatctttgc
     8761 aatcgcttgg cattacaagc gttaacgacc atttttttga tacgctgttg ctcgatacgg
     8821 gtattcggac cgactcgatt gtgcaggccg cgcacgcggc ttcgattaat ttgcgtcatg
     8881 tcagttcgac gcaattggcg atttcgctgg atgaaaccgt gacgccggcc gacctgaccg
     8941 tattggtggg ggcttttgcc aaggcgttga ataaaactcc ggtcgagcct gactggccgg
     9001 gcctggccat gtcaaccgag caatgcattc cagccggttt gcggcgcctt tcgcctattt
     9061 tgacgcatcc tgtattttcg aaaattcagt ccgagaccga catgttgcgt tatttgcgcg
     9121 gcctggcgga caaggatctg gcgcttgacc ggaccatgat tcctttgggt tcgtgcacca
     9181 tgaagctgaa cgcgacggcc gagatgattc cggtgacgtg gcccgaattc ggcgccatgc
     9241 atccttacgc gccggccgac caaaccagag gatatcagga actgatccgc cggttgtcgg
     9301 cggcgttgtg cgaaatcacg ggctatgact cgatcagctt gcagcctaat tctggcgcac
     9361 aaggcgaata ctcgggcctt ctggcgattc gcggctacca tcgcgccaat ggtcagggac
     9421 agc
//
LOCUS       DATCXU010000399         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_448757,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000399 DATCXU010000000
VERSION     DATCXU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448757"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1197
                     /locus_tag="VIR04_08940"
     CDS             181..1197
                     /locus_tag="VIR04_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY9460913.1"
                     /translation="MRFTRRSFMLSTAGAGAAIIATPFRNALAATTFRYKFANNLPAT
                     HPMNIRAREMAKKIQDETGGRFKLQVFPSSQLGSDTETLNQLRAGAVEFFTLSGLILS
                     TLVPAAAISGIGFAFPNYDTVWKAMDGKLGGYIRGEIQKSGLVVMDKIWDNGFRQMTT
                     STKPITTPDDFHDLKVRVPVSPLWTSMFKALKAAPTSINFNEVYSALQTKVADGQENP
                     LAIIQTAKLYEVQKYCSLTNHMWDGFWFLGNKDAWNRLPTDIQTIVAKHVNAAGVLER
                     ADVNTLNTELQKKLTEEKLAFNSPNTGPIRDVLRQAGFYAEWKKKFGEQGWGLLEGAV
                     GSLT"
     gene            1217..>2168
                     /locus_tag="VIR04_08945"
     CDS             1217..>2168
                     /locus_tag="VIR04_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY9460914.1"
                     /translation="MASQASEMQAIAEGPRENRQWVSRLDVLLGHLVEAPVAILVIAE
                     VVILFSGIFARYILNTPLIWSDELASLLFLWLAMFGAAVAFRRGEHMRMTAFVNKASA
                     STRAFLDVFAILTSLAFVVLVLPPSFVATHAQSAITTPAMNLSMGWRTAALPTGLLFM
                     AAFAVLRLIREASRTMLLQALALILIIGAGLYFLSPTLQELGNYNLIVFFVGLVAVGV
                     FAGVPIAFSFGLATFGYLALTTSVPVTVLVGRMDEGMSHLILLAVPLFVFLGLLIEMT
                     SMAHKMVTFLANLLGHVRGGLSYVLVGAMYLVSGISGAKAA"
BASE COUNT          462 a          643 c          573 g          490 t
ORIGIN      
        1 catgttcacc cctgcgttca cgttaatact tcggcaagca gtcccgtttt tgcatctctt
       61 gatagatgat cttcttgctt actcaatgaa ccagatttct tcattattgc cggttccaca
      121 aggcagacct gcaagataca ataaatgttg acaaaaagat cacaacaaag gagacaaaca
      181 atgaggttca cccgccgttc attcatgctc tcgactgccg gcgcaggcgc cgccatcatc
      241 gcaaccccgt ttcgaaacgc cctggccgcc acgaccttcc gttataaatt cgccaataac
      301 ctgcccgcca cccacccaat gaatatccgg gccagggaaa tggcgaaaaa aatacaggat
      361 gaaacaggcg gccgtttcaa actgcaagta ttccccagca gccaactggg atcggatacg
      421 gaaacactca accagttgcg cgcgggcgcg gtcgaattct tcacgctgtc cggtttgatt
      481 ctgtcgaccc ttgtgccggc cgccgcaatc agcggcatcg gctttgcctt tcccaattac
      541 gacacggtct ggaaagccat ggacggcaaa ctgggcggct atatccgcgg ggaaatccag
      601 aaaagcggcc tggtggtcat ggacaaaatc tgggacaacg gtttccggca aatgacgacc
      661 agcaccaaac ccataaccac gcccgatgac ttccacgatc tgaaagtccg ggttcccgtc
      721 agtccccttt ggacatccat gttcaaagcg ctcaaagccg cgcccaccag cataaatttc
      781 aacgaggtct actccgcgtt gcagacgaaa gtcgccgatg gacaggaaaa cccgctggcc
      841 ataattcaga cggccaagct gtacgaagtg caaaagtatt gctcgctgac aaaccacatg
      901 tgggacggct tctggttcct gggcaacaaa gacgcctgga accgcctgcc cacagacata
      961 caaaccattg tcgccaaaca cgtgaatgcc gccggcgtgc tcgagcgcgc ggacgtcaat
     1021 actctgaaca ccgaactgca gaagaagctt accgaagaaa agctggcttt caatagcccg
     1081 aataccggac ctattcgcga cgtgttgcgc caggcgggct tttatgccga gtggaaaaag
     1141 aaattcggcg aacaaggctg gggcctgctg gaaggtgctg tgggctcgct gacttagact
     1201 taatcacata cccgccatgg cgtcgcaagc ttccgagatg caagccatcg ccgagggccc
     1261 gcgcgagaac cggcaatggg tatcccgcct ggacgtcctc ctgggacatc tggtcgaggc
     1321 gcctgttgcc attctggtga tcgccgaagt ggtcatttta ttttcgggca tcttcgcccg
     1381 ctatattcta aataccccgc tgatctggtc cgatgaactg gcctctctgc tgttcctgtg
     1441 gctggccatg tttggcgcgg ccgtggcctt ccggcgcggc gagcacatgc gcatgaccgc
     1501 gtttgtcaac aaggcatcgg ccagtacgcg tgcgtttctg gatgtcttcg caatcctgac
     1561 ttcacttgcc ttcgtcgtgc tggttttgcc gccaagcttc gttgccaccc atgcccagtc
     1621 ggcgatcacc acacccgcca tgaaccttag catggggtgg cgtacggccg cgctgcccac
     1681 cggtctttta ttcatggctg cctttgcggt gctgcgactg atcagagagg ccagccggac
     1741 gatgctgctg caggcgctgg cgctcatcct gatcatcggc gcgggactgt attttttaag
     1801 ccccacgctt caagaattgg gcaattacaa cctgatcgtc ttcttcgtcg ggctggtcgc
     1861 ggtcggcgta ttcgccggcg ttcccattgc attttcattc ggtctggcca ctttcggcta
     1921 cctggcgctg accacatctg tgccagtcac cgttctggtt ggccgcatgg atgaaggcat
     1981 gtctcatctt attttgctgg ccgtgccgtt attcgtgttt ctgggcttgt tgatcgaaat
     2041 gaccagcatg gctcacaaaa tggttacctt cctggccaat ctgctgggcc atgtacgcgg
     2101 cggcctgtcg tatgtgctgg tgggcgccat gtatctggtg tcgggcattt ccggggcgaa
     2161 agccgccg
//
LOCUS       DATCXU010000400         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_748233,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000400 DATCXU010000000
VERSION     DATCXU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748233"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VIR04_08950"
     CDS             complement(<1..241)
                     /locus_tag="VIR04_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460915.1"
                     /translation="MDAITYPNSGSPRNTPADVLTAHLTGDNMCRMGGIYPRQGSAPV
                     LALCRELIAAGYDPSAQLQVYRGSTLCLLVRSVGEA"
     gene            complement(318..593)
                     /locus_tag="VIR04_08955"
     CDS             complement(318..593)
                     /locus_tag="VIR04_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460916.1"
                     /translation="MSAKTIVEPRLRFADLVERKIVANRQSLKNWIDREGFPVGKRLG
                     SNSRSWTESEVQRWIDGRTSDLKPSPNRQRIRKGGRQRKHAADHATA"
     gene            complement(693..1898)
                     /locus_tag="VIR04_08960"
     CDS             complement(693..1898)
                     /locus_tag="VIR04_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEY9460917.1"
                     /translation="MRKTLTDVGVKALKARSERYAFPDPELRGHYVRVQPSGAKAFVT
                     VARNPSGKQIWSVIGAADVMGIDAARDKAREAINRIRRGLPAFEAPPDQPDSFQAVAE
                     NWLKRHAAAKRLRSQKQIERLLRVHVYPVWSKRPFLSIRRSDVAALLDGIEDSTSARQ
                     ADCVLTVIRSVMNWYSVRNDDYTPPIVKGMRRQNPKEHQRDRVLTDAEIRLIWKQAET
                     NGSFGAFVRLALLTAQRRAKLATLRWSDIVDGEWIIPKAPREKDSAGSLLLPKAALEI
                     INAQPKLANPFVFAGRADRAFNGYSKMKRRFDAALPRDAVAAWTIHDLRRSSRSLMAR
                     AGIDQHIAEKIMGHATPGIESVYNRYEFGDEKSAALKRLAQLIDNIVNARANVVPLAK
                     KQRKFRSAP"
BASE COUNT          395 a          617 c          596 g          492 t
ORIGIN      
        1 ccgcctcgcc gacggaccgg accaaaaggc aaagggtgct accgcggtaa acctgtagct
       61 gggcgctggg atcgtatccg gcggcgatca actcgcgaca caaggccagg acgggcgcgg
      121 atccttgccg cggatagatg ccacccatcc gacacatgtt gtcgcccgtt aggtgagcgg
      181 tcagaacgtc ggcaggcgta ttgcgcgggg aaccgctatt ggggtatgtg atggcgtcca
      241 tgtaacttcc tttgatgttc caagcgtcag ggaaagcatc cgcgccccgg gttcacctcc
      301 ggggcgtttt ccttttgcta agcggtggcg tgatcagcgg cgtgcttgcg ctgccgtccg
      361 cctttgcgaa ttctttgccg atttggtgat ggcttcaggt ccgacgttct gccgtcgatc
      421 cagcgttgaa cctcagactc cgtccatgac cggctattgc tgccgagtcg tttcccgact
      481 ggaaaacctt cccgatcaat ccagtttttc aggctttgac gattggcgac aatcttgcgc
      541 tcaaccagat cggcgaagcg caagcgtggc tcaacgatgg tttttgcaga catggccaga
      601 cactcccgtt tgattcgggt gtatgactca tgcgttgaat tgtcagcaaa ggaccggggt
      661 aggtgttaat gacttgatat cacttgagaa ccctatggtg cactgcggaa ttttctctgc
      721 tttttcgcca gtggcaccac gttggcgcgc gcgttgacga tgttgtcaat taattgtgcc
      781 agccgtttca atgcggcgga tttttcgtcg ccgaattcat atcgattgta aaccgactcg
      841 atgcccggcg tggcgtggcc cataattttt tcggcaatgt gttgatcgat accggctctc
      901 gccatcaatg atctgctcga ccgtctcaaa tcgtgaatcg tccaagccgc aactgcatca
      961 cgcggcaagg cggcatcaaa gcggcgtttc attttcgaat aaccgttgaa ggcgcgatcg
     1021 gcgcgaccgg caaacacgaa agggttggca agcttcggct gcgcgttgat gatctcgagc
     1081 gccgccttgg gcagcagcaa tgatccggcg ctatcctttt cgcgtggtgc tttcggaatg
     1141 atccattcgc cgtcaacaat gtccgaccat cgcagcgttg ccaacttggc gcggcgctgt
     1201 gcggtcaaca gcgccagccg aacgaatgcg ccaaagcttc cattggtctc ggcctgtttc
     1261 cagatcaggc gaatttccgc atcggtcagc acgcggtcgc gttgatgctc tttcggattc
     1321 tgccgccgca tgcctttcac gatcggcggc gtgtaatcat cattgcgcac gctgtaccag
     1381 ttcatcaccg agcggatcac ggtcagcacg caatcagctt gccgggcgct ggtggaatcc
     1441 tcgatgccgt cgagcagcgc ggcgacgtca ctgcgccgga tggacaagaa tggtcgcttg
     1501 ctccacaccg ggtaaacatg cacgcgcagc aagcgctcga tctgtttctg cgagcgcaac
     1561 cgcttcgccg ccgcatgacg cttcaaccaa ttctcggcga ccgcttgaaa gctatctggc
     1621 tgatcgggtg gcgcttcgaa tgccggtagc ccgcggcgaa tgcggttgat cgcctcgcgc
     1681 gccttgtccc gcgccgcatc gatgcccatc acatcggcgg caccaatcac gctccaaatt
     1741 tgtttgccag atggattgcg cgcgacggtg acgaatgcct tggccccgct tggctgcacc
     1801 cggacatagt gcccgcgcaa ttccggatcg gggaacgcgt agcgttccga tctggctttg
     1861 agcgctttca cgccaacatc ggtcagagtc ttgcgcatgg aattcccccc ttagggctaa
     1921 cagcgggcta acattcgggc ggtatcaacc cgcgtcaaac cggctaacac ccggctaaca
     1981 aataccctat tttctggcac ttttccattt ttacttttat cgggttttat tgggttttgc
     2041 gccggaccgt cagccttcta agctgagggt cactggttcg aatccagtcg gggtcgccaa
//
LOCUS       DATCXU010000401        13821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1129267,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000401 DATCXU010000000
VERSION     DATCXU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 13821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13821
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129267"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1603
                     /gene="argH"
                     /locus_tag="VIR04_08965"
     CDS             176..1603
                     /gene="argH"
                     /locus_tag="VIR04_08965"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY9460918.1"
                     /translation="MSKNTPPSDQNQFANKAHAWSARFSEPVSDLVKRYTASVGFDQR
                     LASFDIQGSLAHADMLASIGIISRDDKTAIDRGMAQILKEIKAGEFRWSIDLEDVHLN
                     IEKRLVELIGDAGKRLHTGRSRNDQVATDIRLWLRNEIDASADLLRQLRHRLAELASK
                     HTTTILPGFTHLQVAQPVTFGHHLLAYAEMFGRDAERLLDCRKRVNTLPLGAAALAGT
                     SYPIDRERVARTLGFDKVCRNSLDAVSDRDFAVEFCSAAALIMMHISRLSEELVLWMN
                     PRVGFIDLADRFCTGSSIMPQKKNPDVPELARGKTGRVYGHLMALLTLMKGQPLAYNK
                     DNQEDKEGLFDSADTVRDTLTIFIDMVGGIKVKADAMRAAALQGFATATDLADYLVKK
                     GIPFRDAHETVAHAVRTCDEKRCDLTDLTLAELQEFHPAIEQDVYGILTLEGSVAARN
                     HIGGTAPDRVAQEARRILAETAQGH"
     gene            complement(1606..2922)
                     /gene="rlmD"
                     /locus_tag="VIR04_08970"
     CDS             complement(1606..2922)
                     /gene="rlmD"
                     /locus_tag="VIR04_08970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY9460919.1"
                     /translation="MHEVFDIESLDLQARGVARRGGKVAFVEGALPGERVQARIINSK
                     ASFDIARVERLVHASSQRVEPPCPYFGVCGGCIMQHLEPGAQVAVKQRVLEDALWHIG
                     KLRPGRVLPALHGPSWGYRNRARLSVRMVNKKGGVMVGFRERRSRYVTDMTHCLVLPR
                     HVSSLLVPLRSLIGSMSTPDRLPQIEVSVGDRTTALLLRHLEPLIDSDIALLKTFAAD
                     YDVSWWLQPKGLETIHPLIPGDADKLAYALPEYGLRMHYRPGDFTQVNDAMNRSLVVR
                     ALALLEVTASDRVADLFCGLGNFTLPLATQARFVMGFEGNSVLTDRAQASSRLNSLQD
                     RVQFATLDLFKIEVQWLSGQGYFDKVLIDPPREGAEAVVRSLSAAALADRPKKIVYVS
                     CSPATLARDAGILVHEGGYRLQAAGVVNMFAHTAHVESIAVFDQGI"
     gene            complement(3076..3705)
                     /locus_tag="VIR04_08975"
     CDS             complement(3076..3705)
                     /locus_tag="VIR04_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460920.1"
                     /translation="MNHLKSSPLVKLSVVMAALALQGAAWAATAPDATTTPAATPPAT
                     ASQPANPAAWPGRSKGHSHKAMRGHHQRGAMWVPGYGPLDAAFVQSLALNDTQNALLK
                     DAQTTQKSLRQTRHEAMRDVRKARLEQLKSGKIDPHAAIKETSDRGQAMVNARREINQ
                     KWLAVWDSLDATQQQKIASFLDKRAERRAARAQQRGGSSDAVKSGTSSS"
     gene            complement(3798..4577)
                     /locus_tag="VIR04_08980"
     CDS             complement(3798..4577)
                     /locus_tag="VIR04_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY9460921.1"
                     /translation="MTPILVFDLETIPDANGLRRLNHWGAEKTDAEVVEAALAARLES
                     HGNDFLPLHLQQVAVIGCAFRDDQGFRVKTIGAADDPEAVLLSGFFKTIERYTPRLVS
                     WNGSGFDLPVLHYRSLIHSVQAPRYWDTGEDDRDFKYNNYINRYHNRHIDLMDLLAKY
                     NGRANAPLDDLAKLCGFPGKLGMDGGQVWQAWCGGKADEVRAYCQTDVVNTWLVYCRY
                     RLLTGELDRTAYDSEIALVRDTLNASGAPHWKEFLAAWDQY"
     gene            complement(4574..5374)
                     /locus_tag="VIR04_08985"
     CDS             complement(4574..5374)
                     /locus_tag="VIR04_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY9460922.1"
                     /translation="MYAGTSPSVVTQTTETATRTSRRLLFTVALAAALLAGCASRVTR
                     APVTDLTGETSTRAPSGQTYVVVSGDTLYKIAQEHNVDVATLKRINNISNPNQLRVGQ
                     TLRLSGTGAAVEPNAPVASPVAVVPTIPVKPKEPETPPPASDAGIIHWGWPAKGRIIQ
                     SFNANTKGVDIEGTEGEPIDAAADGKVMYAGNGVRGLGNLVLLGHSDGFITAYAHAQT
                     LLVKTGQKVKKGAKIATIGKTETTSPRLHFEIRRHGTAVDPLAYLPTR"
     gene            complement(5392..6207)
                     /locus_tag="VIR04_08990"
     CDS             complement(5392..6207)
                     /locus_tag="VIR04_08990"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY9460923.1"
                     /translation="MRKPVIPFPPDKGTTNRFGRSSLGRGFSAANSNTRISSSGRRLP
                     GLTTAAKALAPVNMGLNSDRSRGVMVQRLRDQGIQDERVLAAMLAVPRHLFVDEGLAS
                     RAYDDAALPIGHGQTISQPWVVARMISAVCENRMPAKVLEVGAGCGYQAAVWAQFVKE
                     VHAIERIRGLFELARYHLRLLKLISRVRLSFGDGMLGLPGVAPFDAIIVAAAGIKIPQ
                     VLLEQLVLGGRLIAPEGASAQRLILIERTGPNTWHREELESVRFVPLKAGTQL"
     gene            complement(6192..6950)
                     /gene="surE"
                     /locus_tag="VIR04_08995"
     CDS             complement(6192..6950)
                     /gene="surE"
                     /locus_tag="VIR04_08995"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY9460924.1"
                     /translation="MRILVSNDDGYNALGVEALVQALKGLADLTVVAPETNCSGASNS
                     LTLNRPLSVRQAPNDFYFVNGTPSDCVHIALTGLLDFRPDLVVSGINNGANMGDDTLY
                     SGTIAAATEAYLFGIPAMAFSLVKKGWEHLDAAAKVARQMVEQQLAQPFGQPVLLNVN
                     IPSAPFDAIRGIHVTRLGKRHPSEPVVKTSTPYGDPVYWIGPVGTVLDSAYGTDFGAI
                     EQLAVSVTPLSFDLTNYEQLGRVKAWAGSLCASL"
     gene            7074..7718
                     /gene="plsY"
                     /locus_tag="VIR04_09000"
     CDS             7074..7718
                     /gene="plsY"
                     /locus_tag="VIR04_09000"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY9460925.1"
                     /translation="MTSIIYFAAGLSIILTAYILGSIPFAVVISRVMGLQDPRSFGSK
                     NPGATNVLRTGNKTAAALTLLGDGVKGWLAVWLATGLAQRFELSDAIVGLAAIAVFLG
                     HVYPIFLKFKGGKGVATTLGVLFAIQPWLGAATVATWLIVAYATKYSSVAAITSALFA
                     PAFYLFGGNVAWEFSATLCLSIAIISAMLIHRHRANISRLMTGQEGRIGAKKKG"
     gene            complement(7773..8822)
                     /gene="tsaD"
                     /locus_tag="VIR04_09005"
     CDS             complement(7773..8822)
                     /gene="tsaD"
                     /locus_tag="VIR04_09005"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY9460926.1"
                     /translation="MNILGFESSCDETGVAVVSTRKGLLAHALYSQIAMHQEYGGVVP
                     ELASRDHIRRVLPLAREVLRNARVSFSDIDAVAYTAGPGLAGALLVGASVAQSFAWAR
                     DLPAIPIHHLEGHLLSPLLADPSPAFPFVALLVSGGHTQLMRVNYVGDYELLGETLDD
                     AAGEAFDKTAKLMGLGYPGGPALSMLALEGDPASFNLPRPMLHSHDLDFSFSGLKTAV
                     LTRIKSLEKEKGQLTGQQRADLAAATEAAIVDVLAVKSIKAMQQTGLKRLVVAGGVGA
                     NRHLRRHLAQAMLDQGGQVFFPPLDFCTDNGAMIAFAAAQRVNAGLVDLADMGHAFTV
                     RPRWDLRDVCQKAVA"
     gene            8936..10228
                     /locus_tag="VIR04_09010"
     CDS             8936..10228
                     /locus_tag="VIR04_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY9460927.1"
                     /translation="MLEEYFQLHEHGTTVRTEILAGITTFLTMSYIIFVNPAILSGTG
                     MDHDAVFVATCLAAALGTLIMAFLANWPIGMAPGMGLNAFFAFTVVGAMGYTWQQALG
                     AVFVSGTIFVILTVTGIRSWLIEGIPKSLRSAIAAGIGLFLAIIALATSGIVVANPAT
                     KVALGDLTAAPALFAILGFFIIAALDAMKIRGAILIGILAVTALSMLTGANEFHGVFS
                     APPSLAPTFLQLDIAGALHTGFFHVILVFVLVEVFDATGTMIGVAKRAGLIPENKPNR
                     LGRALLSDSTAIVAGSLLGTSSTTAYIESAAGVQAGGRTGLTALTVAVLFFLALFLSP
                     LAGSVPAYATAPALLYVAGLMLRELVEIEWNDVTEATPAALAAVIMPFTYSIANGLAF
                     GFISYVVLKAATGKYRDIHPSTLLVAVLFVIKYTLFPG"
     gene            10344..10718
                     /locus_tag="VIR04_09015"
     CDS             10344..10718
                     /locus_tag="VIR04_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY9460928.1"
                     /translation="MSTIDLSKDTFQAAVQADKPLIIDFWAPWCGPCVGFAPTFKAAS
                     QQHEDITFAKVNTEVEQELAAGMKIRSIPTLMVFRDQVLLFSQAGALSASQLEELITQ
                     VKAVDMAKVHAEIAEKQKQKNA"
     gene            complement(10738..12339)
                     /locus_tag="VIR04_09020"
     CDS             complement(10738..12339)
                     /locus_tag="VIR04_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEY9460929.1"
                     /translation="MISTANLTIQFGAKPLFENVNVKFGEGNRYGLIGANGSGKSTFM
                     KIVGGDLDPTGGSVFLEPGLRLGKLRQDQFAFENERVLDVVLMGHEEMWQIVAERDAI
                     YADTDASEDDYMHAADLEAKFAEYDGYTAHARAGELLLGLGFPVEQHELPMREIAPGW
                     KLRVLLAQALFSSPDVLLLDEPTNNLDINTIRWLEDVLNGYQSTMVIISHDRHFLNQV
                     CTHMADLDYRELRIYPGNYDDYMLASTQARERVAAVNAKAKERVADLQDFVRRFAANK
                     SKSRQATSRLKQIDRIKAGTIEVKPSSRQNPYIRFEQNKALHRLAVTVESIKKSFDKP
                     VIREFSTMIEAGQKVAIIGANGVGKTTLLRLLAGDLKPDDGTVRWSDTADLGYMAQDV
                     SDQFQSSSTLFDWFTRYRQAGDDDQSIRAVLGRLLFSAEDITKPVNVLSGGEKNRMSF
                     GRLMLGRHNVMLMDEPTNHLDMESIESLQYALEKYAGTLIFVSHDRQFVSGVATRIIE
                     IMADGEIVDYPGSYDDYLSSRGVES"
     gene            complement(12473..13402)
                     /locus_tag="VIR04_09025"
     CDS             complement(12473..13402)
                     /locus_tag="VIR04_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY9460930.1"
                     /translation="MASATAMTHAAQPSATGIYLISPSGAVPDPKILDLAQARLAKLG
                     FKTAVDRAALAVHERFAGTDKQRLAGVARAIKQKHSIVMATRGGYGVSRLLPFIDWRA
                     VADSGKIFIGQSDFTAFNLALLAQTGAVSFAGPTALFDFGAKKINDLTTALFVESMHG
                     ELEILSFESPGSDAVDARGVLWGGSLAMVASLAGTAYMPKVRGGILFLEDIGEHPFRI
                     ERMLTQLWHAGVLSRQKAIVLGHFTNYRLATHDNGFDLPSVVRWLRQTVKVPVVTGLP
                     YGHVPLKATLPIGKKVGIATEAGMAYLLLEEHS"
     gene            complement(13430..>13821)
                     /gene="tadA"
                     /locus_tag="VIR04_09030"
     CDS             complement(13430..>13821)
                     /gene="tadA"
                     /locus_tag="VIR04_09030"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY9460931.1"
                     /translation="DARGQLIGAGCNSTIKNHDPTGHAEIMALRQAASMVGNYRLPGA
                     RLFVTLEPCAMCLGAMFHARIERVVYGASDPKTGACGSVLSLHEVTALNHHTQVCGGL
                     LADECGALLRRFFRARRINRKNDHLYL"
BASE COUNT         3080 a         4118 c         3701 g         2922 t
ORIGIN      
        1 atcgccgaga tttgtttcat ggcagatacc ccagttcgat cgtcatcttg ttcaatgctc
       61 cagtttgtgc gcgccacttg aacccgttcg gcggacttca tgcaaaaata actttaaaat
      121 tatcgcacga cggcaaaaca gggtgttttt taacatacct ttataatcgc gcatcatgtc
      181 aaaaaacact ccaccctccg accaaaacca attcgccaat aaagctcacg cctggtccgc
      241 gcgtttttcc gaacccgttt ccgatctggt caagcgctac accgcttcag tgggcttcga
      301 tcaacgactg gccagtttcg acatccaagg atcgttggcg cacgccgata tgctggcatc
      361 gatcggcatc atcagccgcg acgacaaaac ggcaatcgac cgcggaatgg cgcaaatact
      421 gaaagaaatc aaagcgggcg aattcagatg gtccatcgac ctggaagatg ttcacctgaa
      481 tatcgaaaaa cgtctggttg agctcatcgg cgatgccggc aaacggttgc ataccgggcg
      541 ttcccgcaat gatcaggtcg ccaccgacat ccgtttatgg ctgcgcaatg aaatcgatgc
      601 gtcggcggat ttgttgcgtc agttgcgtca ccggctggcc gagctggcct cgaagcacac
      661 cactacgatt ttgccgggct ttacccattt gcaggtggct caaccagtta ccttcgggca
      721 ccaccttttg gcctacgccg agatgttcgg acgcgacgcc gaacgcctgc tcgactgccg
      781 caaacgggtc aatacgctcc cgttgggggc ggcggcgctg gccggcacat cctacccgat
      841 tgatcgcgag cgggtcgcca gaacgctggg gttcgacaaa gtctgccgca attcgctgga
      901 tgcggtttca gatcgcgact tcgccgtcga gttttgctcc gccgcggcgc tgatcatgat
      961 gcatatttcc cggctgtctg aagagttggt gctgtggatg aacccgcgcg tgggttttat
     1021 cgacctggcc gaccgctttt gcaccggcag ttccatcatg ccgcagaaga aaaatcccga
     1081 cgtgccggag ctggcgcgcg gaaaaacggg gcgggtatat ggccacctga tggcgctgct
     1141 cacacttatg aaaggccagc ccctggccta taacaaggat aatcaggaag ataaagaagg
     1201 cttgttcgac tcggccgaca ccgttcgcga cacactgacg atcttcatcg acatggtggg
     1261 aggcatcaag gtcaaggccg acgccatgcg ggcagccgcc ttgcaagggt ttgcgacggc
     1321 caccgacttg gccgattatc tggtaaaaaa gggtattccg tttcgcgacg cgcacgagac
     1381 agtcgcccat gcggtgcgca cctgcgacga aaagcgctgc gatctgaccg acttgacgct
     1441 ggccgaactg caagagtttc atcctgcgat cgaacaggat gtctacggca tattaaccct
     1501 ggaaggctcc gtggccgcgc gcaatcatat aggcggcacg gcgcccgacc gggtcgcgca
     1561 agaagcccga cggattctgg ccgaaaccgc ccaagggcat taacatcaaa taccttgatc
     1621 gaaaaccgca atcgactcga catgggccgt atgtgcaaac atattcacta ctccggcggc
     1681 ttgcaagcga tagccgcctt catgaacgag tattccggcg tcgcgcgcca aagtagcggg
     1741 gctgcacgat acatacacta tcttttttgg ccggtcagcc agggccgccg cgctcagtga
     1801 ccgcacaacg gcttcggccc cttcgcgcgg agggtcaatc agaaccttgt caaaatagcc
     1861 ctgcccggac agccactgca cctctatctt gaacaagtcc agcgtggcga attgcacccg
     1921 atcctgcaaa ctgtttagcc tggacgaagc ctgagcgcga tccgtcagaa ccgaatttcc
     1981 ttcaaaaccc atcacaaagc gcgcttgtgt ggccaaaggc aaggtaaagt tgcccaagcc
     2041 gcagaacaga tccgccacac gatcgctggc tgtaacctcg agcaaggcca acgccctgac
     2101 gaccaggcta cggttcatcg cgtcgttgac ttgcgtaaaa tcgcccggcc gataatgcat
     2161 gcgcaagcca tactccggca atgcgtaagc cagtttgtcg gcgtcgcccg gaatcaatgg
     2221 atggatggtt tccagccctt tcggctgcaa ccaccacgac acatcgtaat ccgcggcaaa
     2281 cgtcttgagc agagcgatgt cggaatcgat cagcggctca agatggcgca gcaataacgc
     2341 agtggtccga tcaccgaccg acacctcaat ctgcggcaga cgatcggggg tcgacattga
     2401 tcctatcagc gagcgcagcg gtaccagcaa gctggatacg tgccgcggca acaccaggca
     2461 gtgcgtcata tcggtcacat agcggctgcg gcgctcgcgg aacccgacca tgacgccgcc
     2521 ctttttgtta accatgcgca cggacaaacg cgcccgattt cgatagcccc agctcgggcc
     2581 gtgcaaggct ggcaatacac gcccgggacg cagcttgcca atatgccaca acgcgtcttc
     2641 aagaacgcgc tgcttgacgg caacctgcgc gccaggctca agatgctgca tgatgcaacc
     2701 gccgcacact ccaaaatacg gacacggcgg ttcaacccgc tgtgatgaag catgtacaag
     2761 ccgctcaacg cgggcaatgt cgaacgatgc cttgctgttg ataattcgcg cctgaacccg
     2821 ttcgccgggc aatgctcctt ccacaaaagc cacttttccg ccgcggcgcg ccacaccgcg
     2881 cgcctgaagg tccaaagatt caatatcaaa cacttcatgc ataaagattc aagcgtcccg
     2941 gttcagagtc aggttcaagt gataaagcca aatacagctt gtgcccaacg aagctaaaag
     3001 cgtcattggg cacaagccag cccctgccac gttaattgaa cactgcaaga cagacttgaa
     3061 gattgcagcc ttggcttacg aggaggacgt gcctgatttt accgcgtcgg aactgccacc
     3121 gcgctgctgg gcacgcgccg cccgcctctc tgcgcgctta tccagaaacg acgcaatttt
     3181 ttgctgctgc gttgcgtcca gcgagtccca taccgccagc catttctgat taatttcgcg
     3241 gcgcgcgtta accattgcct ggcctctgtc cgacgtttcc tttatcgccg catgcggatc
     3301 gatcttgccc gatttcaatt gctccagccg cgctttacga acgtcgcgca tcgcttcatg
     3361 ccgagtctgt cgaagacttt tctgcgtggt ctgcgcatct ttcagcaagg cgttctgcgt
     3421 atcattgagc gccaaagatt gtacaaacgc ggcatccagc ggcccatagc cgggaaccca
     3481 cattgcgccg cgctgatgat gaccgcgcat tgctttgtgc gaatgaccct ttgatctgcc
     3541 gggccacgcc gccgggttgg cgggctgact ggccgtagcc ggcggcgtgg cggcgggggt
     3601 ggtggttgcg tcgggggccg ttgccgccca ggccgcgccc tgcaaagcaa gagcagccat
     3661 cacaaccgac agtttgacaa gaggcgaaga tttcaggtgg ttcataatga tccattcctt
     3721 tagatgagga gcggccatta tctgccggca acgatttcca acctgtttcg ccgcccatga
     3781 attgctttca attgatttca gtactggtcc cacgcagcca gaaattcttt ccagtgaggc
     3841 gcgccgctgg cattaagggt gtcgcgcaca agcgcgatct cgctatcgta agcggtccgg
     3901 tcgagttcac cggtcaacag gcgatagcgg caatacacca gccaggtgtt cacgacatca
     3961 gtctggcagt aggcacgcac ctcgtcagcc ttgccgccac accatgcctg ccatacctgg
     4021 ccgccatcca tgcccagctt gcccggaaag ccgcatagct tagccagatc gtccagcggc
     4081 gcattggcgc ggccattgta tttcgccaat aaatccatca ggtcgatatg acgattatgg
     4141 tagcggttaa tgtagttgtt gtatttgaaa tcgcgatcat cttcgcccgt atcccaatac
     4201 cgcggcgcct gcactgaatg aatcaggctg cggtaatgca gcacgggcaa atcaaaacct
     4261 gacccgttcc aactgaccag acgtggcgta tagcgttcaa tcgttttgaa aaagcctgat
     4321 aacaggactg cttcagggtc gtcagcagcg cctatcgttt taacgcgaaa cccctgatcg
     4381 tcccggaagg cgcacccaat tacggcaact tgctgcaaat gcaaaggtag aaaatcgttg
     4441 ccatgcgatt ccaggcgggc cgccagcgca gcctcgacca cctcggcatc ggtcttttcg
     4501 gcgccccagt gatttagccg gcgcagcccg tttgcatcgg gaatcgtctc gaggtcgaaa
     4561 acaagtatgg gggtcatcgc gtgggcaagt aagccagcgg atcaacggcg gtgccgtggc
     4621 gacgaatctc aaagtgcaag cgcggcgaag tggtctcggt ctttccgatt gttgctattt
     4681 tcgccccctt cttcactttc tgccccgtct tcaccagtaa cgtctgggca tgcgcgtagg
     4741 cggttataaa gccatcgcta tggcccagca agaccaggtt tccgagccct cgcaccccat
     4801 tgccggcata catgaccttg ccatcggcgg cggcgtcaat cggttcgccc tcggttccct
     4861 cgatgtcgac ccccttggtg ttcgcgttaa agctttgaat aatgcggcct tttgccggcc
     4921 aaccccagtg aatgatgcct gcatcgctgg cgggcggcgg tgtctcaggc tctttgggtt
     4981 tgacggggat tgtcggaact accgccaccg gggacgctac cggtgcgttg ggttcgacag
     5041 cggcgcccgt gccacttagc cgcaaagttt gcccaacgcg caactgattc ggattgctga
     5101 tgttgttgat ccgcttcaat gtggcaacat ccacattatg ctcctgggcg attttgtaca
     5161 gagtgtcgcc cgacacaacg acatatgttt ggccagatgg cgcccgagtg ctggtttctc
     5221 ctgtcaggtc agtaacaggg gcgcgggtca ccctggaggc gcaacctgcc agcaacgccg
     5281 ctgccaacgc aacggtgaac aataagcggc gagacgttcg tgtggcggtt tcagttgtct
     5341 gagtgacgac agacggtgac gtcccagcat acatatggtt ctcctaaaga cctatagttg
     5401 agtccccgcc ttcaggggaa caaagcgcac cgattccaat tcttcgcgat gccacgtatt
     5461 gggtccggtg cgttctatta aaataaggcg ctgtgccgag gccccttcag gcgcaatcaa
     5521 acgcccgcca agcacaagct gctccagcaa aacttgcggt attttaatac cagcggcggc
     5581 gactatgatt gcgtcaaacg gcgcaacgcc gggcaagccc agcattccat cgccaaacga
     5641 cagacgcacc cggctaataa gcttaagcaa gcgaagatga tagcgcgcga gttcaaacag
     5701 ccctcgaata cgctcgatgg catgcacttc cttgacgaat tgagcccata cagccgcctg
     5761 atacccgcaa cccgccccaa cctccaggac tttcgcgggc atgcgattct cgcacaccgc
     5821 cgaaatcatg cgcgcaacca cccacggctg tgaaatagtc tggccatggc caataggcaa
     5881 tgcggcatcg tcgtaggccc ggctggccag cccttcatca acaaacaagt ggcgcggcac
     5941 agccagcatt gcggccagca ccctttcgtc ttgaatcccc tgatcgcgca agcgctgaac
     6001 catcacgccg cgcgaccggt ccgagttcaa gcccatattg acaggcgcca aagctttggc
     6061 cgccgtggtt agcccaggca gacgccggcc ggatgacgag atgcgcgtgt tgctgttggc
     6121 agcggaaaac ccacgcccca gactggaacg gccgaagcga ttcgtcgtcc ccttgtcagg
     6181 gggaaacgga attacaggct tgcgcataag gagcccgccc aggccttcac gcgacccaat
     6241 tgttcgtaat tggtcagatc gaaacttaag ggagtaaccg agacggcaag ctgctcaatg
     6301 gcgccgaagt cagtgccgta ggctgaatcc agaaccgtgc ccacaggccc gatccaatat
     6361 acaggatcgc catacggagt actggttttt acgaccggct cggacggatg ccgtttaccc
     6421 aggcgggtga catggattcc ccgaatcgca tcgaaaggcg ccgacggaat gtttacattc
     6481 agcaagaccg gctgcccgaa cggctgcgca agttgctgct cgaccatttg ccgcgcaacc
     6541 ttggctgccg cgtccagatg ttcccagccc ttcttcacca gcgagaacgc catggcgggt
     6601 attccgaaca gatacgcctc ggtggcggcc gcaatagtgc cagaatacaa tgtgtcgtcg
     6661 cccatattgg caccgttatt gatgcccgac acgacgagat caggccgaaa atcgagcaaa
     6721 ccggtcaatg caatatggac gcaatcagag ggagtgccat tgacgaagta aaaatcattg
     6781 ggcgcttgcc gcaccgacaa tggccgattc aatgtaagcg aattcgatgc gccgctgcag
     6841 ttcgtttcgg gcgctacaac ggtcagatcg gccaagccct tcagggcttg caccagcgcc
     6901 tcgacgccca aagcgttata accgtcgtca ttagatacta aaatgcgcat cttgcttcca
     6961 taaaattcaa gtcggcttga acgattgtac ccggtcgctt tgtggttttg ggcggcgctc
     7021 acggtagaat ctaaactatg cgcgcttccc aagcgcgttt tggatatacc tgcatgacat
     7081 caataattta tttcgccgcc ggcctgtcaa tcattctgac cgcttacatt ttgggttcca
     7141 ttccgtttgc agtcgttatc agccgggtca tggggctgca ggatccccgc agcttcggct
     7201 ccaaaaaccc gggtgccacc aatgttttgc gcacagggaa taaaacggca gcggcattaa
     7261 cactgcttgg cgatggcgtc aaaggctggc ttgccgtgtg gctggctacg ggactggcgc
     7321 aacgtttcga gctgtcagac gccattgtcg gcctggctgc catcgcagta tttctgggcc
     7381 acgtgtaccc gatttttttg aagttcaaag ggggcaaagg ggtggccaca acgctgggtg
     7441 tgctctttgc catacaacca tggctgggcg ccgcaacagt ggcaacctgg ctgattgtcg
     7501 cttacgccac caagtactct tcagtagcgg ccatcacgtc agcccttttc gcgcccgcgt
     7561 tttacttgtt tggcggaaat gtcgcatggg aattcagtgc gaccctgtgt ctgtcgattg
     7621 cgatcataag cgccatgctg atacatcgcc accgcgccaa catcagtcgc ctgatgacgg
     7681 gccaagaagg ccggatcggc gccaaaaaga aaggctagct agcccaagcc ggagccggtc
     7741 aagagccatc atgccgcgaa cggaagcagc gcttacgcta ctgccttttg acagacatcg
     7801 cgcagatccc accgcggccg cacggtaaac gcatgcccca tatccgccag atcaaccaat
     7861 ccggcattaa cccgttgagc cgccgcaaag gcaatcatgg cgccattatc ggtgcaaaaa
     7921 tccagaggcg gaaaaaatac ctggccgcct tgatcaagca tggcctgggc caagtgccgg
     7981 cgcaaatgac gattggcccc cactccccct gccacgacaa gacgtttaag tcccgtttgc
     8041 tgcatcgcct tgatcgactt gaccgccagt acatcgacga tcgccgcttc ggtcgcggcc
     8101 gcaagatcgg cgcgctgctg tccagtcaac tggccctttt ctttttccag gctctttatg
     8161 cgcgtcaata cggccgtttt taacccactg aagctgaagt ccagatcgtg gctgtgcaac
     8221 atcgggcgcg ggagattaaa gctggccgga tcgccttcaa gagccaacat ggacaacgcg
     8281 gggccgcccg ggtatcccag ccccatgagc ttggctgtct tatcgaacgc ctcgcccgcc
     8341 gcatcgtcga gcgtttcgcc caacaattca taatctccca cgtaattgac acgcatgagc
     8401 tgggtatgac cgcctgacac cagcaaagca acaaaaggga aagccggcga cggatcggcg
     8461 agcaaaggcg acagcaaatg tccttcgagg tgatgaatgg gaatggcggg caaatcgcgc
     8521 gcccacgcaa acgactgggc cacgctggca ccgaccagca gtgcgccggc cagtccggga
     8581 cccgcggtgt acgccactgc gtcaatatcg ctgaatgaca ctcgcgcgtt gcgcaatacc
     8641 tcgcgcgcca atggcaaaac acgcctgatg tggtcacgcg aagcaagctc gggaacgacg
     8701 ccgccgtact cctgatgcat cgcaatttga ctgtacagag catgcgccag caaacctttg
     8761 cgagtgctca ccactgctac gccggtttca tcgcaagaac tttcaaaacc aagaatattc
     8821 atggctgcaa gtgtacaacc acgagcaaaa ccgccttaga attgcgatca acttttgcct
     8881 tttttcagct ttatttcaaa tttcagccga tatatcggcc aatatgggaa caccaatgct
     8941 tgaggaatat tttcaactgc atgaacacgg gaccacggtg cgcaccgaaa tcctggcggg
     9001 catcaccact tttctgacca tgtcctacat catttttgta aacccggcga ttctttcggg
     9061 taccggcatg gatcacgacg ccgtcttcgt cgccacctgc ctggcggccg cgttaggcac
     9121 gctaatcatg gcctttctgg caaactggcc gattgggatg gctccgggca tgggtttgaa
     9181 tgcatttttc gcatttaccg tcgtgggcgc catggggtac acctggcagc aagccctggg
     9241 cgcggtattc gtctctggca ccattttcgt cattctgacc gtcaccggaa tccgatcctg
     9301 gctaatcgag ggcattccca agtcgttgcg atcggccatt gcggcaggta taggactttt
     9361 tctcgccatc attgcgctcg ccacctccgg cattgtcgtc gccaatccgg cgaccaaagt
     9421 cgctttgggc gatcttacgg ccgcgccggc gcttttcgcg atactcgggt tcttcatcat
     9481 tgccgcgctc gacgccatga aaattcgcgg cgccatcctg attggcatcc tggccgtcac
     9541 agcattatcc atgcttaccg gcgccaacga atttcacgga gtgttttccg cgccccccag
     9601 tttggcgccc accttcctgc agcttgatat agcgggcgcc ttgcacaccg gattttttca
     9661 tgtcattttg gtcttcgtgc tagtcgaagt gttcgatgcc acgggcacca tgataggcgt
     9721 cgctaagcga gcgggcctga tcccggaaaa caagcccaat cgcctgggtc gcgcgctgct
     9781 ctcggacagc acggccatcg tggccggctc gctgctgggc accagcagca cgaccgccta
     9841 catcgaaagc gcggccggcg ttcaagcagg cgggcgcacg ggccttacgg cgctgactgt
     9901 ggccgtgttg tttttcctgg ctttatttct ttccccgctt gcgggctccg ttcccgccta
     9961 cgctaccgcc cctgccctgc tgtatgtggc cggcctgatg ttgcgcgaac tcgtcgaaat
    10021 cgaatggaac gatgtcaccg aagcaacgcc ggccgcgctt gccgccgtca tcatgccgtt
    10081 tacgtactcc atcgccaacg gcctggcgtt cgggttcatc agctatgtgg tgcttaaagc
    10141 ggccactggg aaatatcgag acatccatcc gtcgaccctg ctggttgccg tgttgttcgt
    10201 gatcaaatac acccttttcc caggatgaag agcggaatat cgccgtgaaa aagcaataca
    10261 ctatactttg acatgttgcc ttcacgtata agcggaacct tttcaacaag cggctgatag
    10321 ccgcttttta tttcggactt tccatgagca ccatagatct aagcaaagat actttccagg
    10381 ccgctgttca ggcagacaag cctttaatca ttgatttctg ggcgccttgg tgcggcccat
    10441 gcgtcggctt tgcacccacg ttcaaagccg catcgcagca acacgaagac attaccttcg
    10501 caaaggtcaa caccgaagtc gaacaagagc ttgcggccgg catgaagata cgctccatcc
    10561 cgactttgat ggtgtttcgc gatcaggtgc tattgttttc ccaggcgggc gcactttcgg
    10621 caagccagct cgaagagctg attacccagg tcaaggctgt ggatatggca aaagtccacg
    10681 ccgaaatcgc ggaaaagcaa aaacagaaaa acgcttagtt gcggcttgga aagcccttca
    10741 ggattccact ccgcgcgaac ttaaatagtc atcatagctg cccgggtagt cgacgatctc
    10801 gccgtcggcc atgatttcta tgatgcgcgt tgcgactccg gagacgaatt gccgatcatg
    10861 cgagacaaat atcaacgtgc cggcatattt ttcaagcgcg tactgcaacg actcaatcga
    10921 ctccatatcc aggtggttag tgggctcgtc catcaacatg acgttgtgac gtcccagcat
    10981 caggcgaccg aaactcattc tatttttttc gccgcccgaa agcacattga cgggcttggt
    11041 aatgtcttcg gccgagaaca acagccgccc caataccgcg cgtatggatt ggtcatcgtc
    11101 gcccgcttga cgataacggg tgaaccagtc gaacaacgtc gaagaagact gaaactgatc
    11161 ggaaacatct tgcgccatat atcccagatc ggcggtgtcg gaccatctga ccgttccatc
    11221 gtctggcttc aaatcgccag cgagtaaacg cagcaatgtc gttttgccca ccccatttgc
    11281 gccaatgatg gcaactttct ggccggcctc aatcattgtg gaaaactcgc ggataactgg
    11341 cttgtcaaac gatttcttaa tcgactcgac ggttaccgcc aggcgatgga gcgctttgtt
    11401 ctgttcaaag cggatgtagg gattctggcg cgacgatggc ttgacctcga tcgtaccggc
    11461 tttgatgcga tcaatctgct tcaggcgcga ggtagcctga cgcgacttcg atttgttggc
    11521 ggcaaaacgg cgcacgaaat cctgcaagtc agccacgcgt tcttttgctt tcgcgttgac
    11581 ggcggcgacg cgttcgcgcg cctgggttga cgccagcatg taatcatcgt aattgcccgg
    11641 ataaatgcgc agttcccgat aatccagatc ggccatgtgc gtacagacct ggttcaggaa
    11701 atgtcggtcg tggctgataa ttaccatagt gctttgataa ccattgagca catcttcgag
    11761 ccagcgaatg gtattgatgt ccagattgtt ggttggctcg tcgagcaaca aaacgtcggg
    11821 gctcgagaat aaagcctgcg ccagaagcac gcgcaatttc caaccggggg cgatttcgcg
    11881 catgggcaat tcatgctgct ccacgggaaa gcccagtccc agtaacagct ccccggcgcg
    11941 cgcatgcgcg gtataaccat cgtactcggc aaatttggct tcaagatcag ccgcatgcat
    12001 gtaatcgtct tcactggcat cagtgtcggc ataaatggcg tcacgctccg ccacaatttg
    12061 ccacatctct tcgtggccca taagaaccac gtccagaacc cgttcattct caaaggcaaa
    12121 ctgatcctga cgcaactttc ccagacgcaa gccgggttcc agaaaaacac tgccgccggt
    12181 gggatccaga tcgccgccaa caattttcat gaaggtggat ttaccggagc cattggcgcc
    12241 gatcagtccg taacgattgc cctcgccgaa tttgacgttg acattttcaa ataaaggctt
    12301 ggcgccaaac tgaatagtga gatttgcggt ggagatcacg gagagttatc gcgtattgaa
    12361 gatttggatt aaagcggaaa atcgcgcccg ccgggcgcga ttcaattgat gagtaacctg
    12421 taagtgtaac aacttcgcca ccccaatgtt cagggtggcg atgaacgcgc gcctaagaat
    12481 gttcttcgag cagcaaataa gccatgccgg cttccgttgc aatacccacc ttctttccga
    12541 tcggcagcgt agccttcagt ggaacatgac cataaggcaa gccggtaaca accggaacct
    12601 tgaccgtctg gcgcaaccag cgaaccactg acggaaggtc gaacccatta tcgtgagtcg
    12661 ccaggcggta gttggtgaaa tgacccagca ctatggcttt ctggcgcgac agcacgcccg
    12721 catgccagag ttgggtcagc atgcgctcaa tgcgaaacgg atgctcgcca atatcctcca
    12781 gaaacaagat gccgcctcgc actttcggca tataagcggt gcccgcgagc gacgccacca
    12841 tggccaggct tccgccccac agaacgccgc gcgcatccac tgcgtccgag ccgggggatt
    12901 cgaaactcag gatttccagt tcgccatgca tgctctcgac gaacaaggca gtcgtcaggt
    12961 catttatttt tttggcgccg aaatcaaaca aagccgttgg gccggcaaaa ctaacggctc
    13021 ccgtctgtgc caacaaggca agattgaatg ccgtgaaatc gctttgacca ataaagatct
    13081 tgccgctatc ggcgacggcg cgccaatcga taaaaggcaa caagcggctg acgccatagc
    13141 cgccgcgcgt ggccatgaca atggagtgtt tttgctttat tgcgcgcgcg acgcccgcca
    13201 ggcgctgttt atctgtgccc gcaaagcgtt catgaacggc cagcgccgcg cggtcaaccg
    13261 ctgttttaaa gcccagcttg gccagacggg cttgagccag gtcgagtatt ttcgggtcgg
    13321 gcaccgcgcc cgacggcgaa attaaataaa tgccggttgc cgatggttgc gccgcatgcg
    13381 tcattgctgt agctgatgcc attactataa aaacctcgca aacgaagggt cacaagtata
    13441 gatgatcatt ttttctattg atgcgccgcg cgcggaaaaa ccgccgcaac aaagcgccgc
    13501 attcatccgc caacaacccg ccgcatacct gcgtatggtg attcagcgct gtcacctcat
    13561 gcaggctcag gacgctgccg caagctccgg tttttggatc gctggcgcca tacaccacgc
    13621 gctcgatgcg ggcatggaac attgcgccca ggcacatggc gcaaggctcg agcgtcacaa
    13681 aaagccgggc accaggcagg cgatagttgc ccaccatgct ggctgcctgg cgcagcgcca
    13741 ttatttcggc gtgcccggtg ggatcatgat ttttaattgt gctgttacag cccgcgccga
    13801 tcaactgtcc gcgagcatcc a
//
LOCUS       DATCXU010000402         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1059378,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000402 DATCXU010000000
VERSION     DATCXU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059378"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VIR04_09035"
     CDS             complement(<1..1077)
                     /locus_tag="VIR04_09035"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY9460932.1"
                     /translation="MFESLNFTGPTVLPDLIPYAGFDDARAATQRLVEIYTRNTAFIR
                     DGFANYAQGKFTHAQRIRACYPAIRIKVSTFQEVDSRLSYGHVAQPGTYMTTVTQPRL
                     YFDYLVEQIGLLIKNHGVPVEIGESDVPIPLHFSFPNGEYVEGVYPEALGDLLRDHFD
                     VPDLAVTDDVIVNGDWQGRPGEPRPLAPFTAPRVDYSLHRLQHYTATAPEHFQHFVLF
                     TNYQYYVDEFCQWARETLAQGEGGYTSLIEPGNVVTPRGPGGERSGPVLERLPQMPSY
                     HLVRENHTGITLINIGVGPSNAKTITDHIAVLRPSVWLMLGHCAGLSTTQRLGDYVLA
                     HGYVREDHVLDADLPNWVPLPPLAE"
     gene            1207..2145
                     /locus_tag="VIR04_09040"
     CDS             1207..2145
                     /locus_tag="VIR04_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY9460933.1"
                     /translation="MSTILNAVLPLFGLILLGYVSGRRQLLGPGAVDNLNNFVVWLAL
                     PALLFQAMAQITWEQANQPGFVLTTGLAMAAVFGTSYVLGRNPRRRLADASIDGLAAS
                     YSNSGYMGIPLCIMVLGQDSLPATVVSVLLTACGLFAFSIVLVEIDLQSSPNILRTAR
                     KVLQSLLRNPLVTAPLLGAVFAAMQIPLPGPLLQLTTLLGEAASPCALVTIGLFLAQS
                     QTGGELKAVRRIVVLKLFLHPLITFVLVRWVFDMPALWGNTAILLSALPVGTGPFMLA
                     KLYDRQPAVTSRAILLSTLLSVFTVSVLVAFLNGGA"
     gene            complement(2142..2576)
                     /locus_tag="VIR04_09045"
     CDS             complement(2142..2576)
                     /locus_tag="VIR04_09045"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY9460934.1"
                     /translation="MGCGRCKQAQTATRARRPYYGLYCKSTQPHSKSKELVFKVRYHV
                     EKGVVMKANLELIGATSALPLLKGWTAVAGRDAIHKEYVFKDFGEAFAFMARVALQAE
                     KLNHHPEWSNVYNKVSVTLATHDAGGVTQRDVALASFMDKLA"
     gene            2683..>3640
                     /locus_tag="VIR04_09050"
     CDS             2683..>3640
                     /locus_tag="VIR04_09050"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800583.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY9460935.1"
                     /translation="MTERTQCKRLQVANVLHQFIENEALPGTGVASAAFWSGFDELMH
                     DLAPKNRALLAERDRLQTEINNWHHRNPGRITDQHAYQDFLKEIGYLKPVPAHVAVNP
                     LNVDVEITEQAGPQLVVPITNARYALNAANARWGSLYDALYGTDAISEEGGATRAGGY
                     NPVRGAKVIAFARHFLDENAPLAQGSHSDATAYTVSNGQLKVTLKNGASSGLKHAEGF
                     VGYRGNASSPEAIVLKHNGLHFEIQIDKTNPIGKQDSAGVKDILMEAALTTIMDLEDS
                     IAAVDADDKVLAYRNWLGILKGDLTEEVSKGGKTFTRALNPDR"
BASE COUNT          791 a         1103 c          988 g          758 t
ORIGIN      
        1 ctcggccagc ggcggcaagg gcacccagtt cggcaaatct gcatccagca catggtcttc
       61 gcgcacatat ccatgcgcca gtacgtagtc gcccagacgt tgcgttgtgc tcaaacccgc
      121 gcaatgacct agcatcagcc acacggacgg gcgcaggacg gcgatatgat cggtgatggt
      181 tttcgcgttg gacgggccga cgccaatatt gatcaaagta atcccggtgt ggttctcgcg
      241 caccagatga taggaaggca tttgcggcag acgttcaagg acaggtccgc tgcgttcgcc
      301 acccggcccg cgcggcgtga cgacattgcc gggctcgatc aacgaggtat agccgccttc
      361 gccctgcgcc aaggtttcgc gcgcccactg gcaaaattcg tccacatagt actgataatt
      421 ggtgaacaaa acgaagtgct ggaaatgctc cggcgccgtc gccgtgtaat gctgcagacg
      481 atgcagcgaa taatccacgc ggggagcggt aaacggcgcc aacggccgcg gttcacctgg
      541 ccggccttgc cagtcgccat ttacgatgac gtcgtcggta accgccagat ccggcacatc
      601 aaaatgatcg cgcaacaaat cgcccagagc ctcgggatac acgccctcga catattcgcc
      661 gttcggaaat gaaaaatgca gcggtatggg cacgtcggac tcgccgatct cgaccggaac
      721 accatgattc ttgatcagca gaccgatttg ttccactaga tagtcgaaat acaaccgggg
      781 ctgcgtcacg gtcgtcatgt acgtgcccgg ttgcgccacg tgaccatacg acaggcggct
      841 gtcgacctct tgaaaggtgc tgactttaat gcggatcgcg gggtaacagg cccggattcg
      901 ttgcgcatgg gtaaacttcc cttgggcata attcgcgaaa ccatcgcgaa tgaaagcggt
      961 atttctggta tatatctcga ccagacgttg cgtcgcggcg cgcgcgtcgt caaacccggc
     1021 gtaggggatc aaatctggca agaccgtcgg accggtaaaa ttcaaggatt caaacatccg
     1081 cctgcaccgt tgaacagttg aaagttgaac tatgacatta aacggcccgc acggcacgcc
     1141 tgatcaagcg gatcgcccgt ttgttttaca tgaatgcgat ccattgccat cacaaaaggc
     1201 cacagattga gcaccatcct taatgccgta ttaccgttgt tcggcctgat tctcctgggc
     1261 tatgtcagcg ggcggcggca acttttgggg ccgggcgccg tcgacaacct gaataatttc
     1321 gtcgtctggc ttgccctgcc ggccctgctg tttcaggcga tggcgcaaat tacctgggag
     1381 caggccaacc agcccggctt cgtgctgacc acgggtcttg ccatggcggc agtctttgga
     1441 acatcgtacg tcctgggccg aaacccgcgc cggcggctcg ccgacgccag catcgacggc
     1501 ctggcggcct cgtattccaa ttcgggctat atgggcatcc cattatgcat tatggttctg
     1561 ggccaggaca gcctgcccgc aacagtcgtc tcggtgctgc tgaccgcctg cggattattc
     1621 gcgttctcga ttgtgctggt tgaaatcgat ttgcaaagct cgcccaatat tttgcgcaca
     1681 gcccggaaag tgctgcagtc tttactgcgc aaccctcttg tgacggcgcc tttgctgggg
     1741 gccgtgtttg cggcaatgca aatccccttg cccggaccat tgctgcaact gaccaccttg
     1801 ctgggcgaag ccgccagccc gtgcgctctg gtcaccatcg gcctgttcct ggcgcaaagc
     1861 cagacaggcg gcgagcttaa agccgtgcgg cgcattgttg tgcttaaatt gtttttacat
     1921 cccctcatca cgttcgttct ggttcgctgg gtcttcgaca tgcctgcgct ttggggcaat
     1981 accgccatcc tgcttagcgc cttgccggtg ggcacggggc cattcatgct ggccaagctc
     2041 tacgaccggc agcccgcggt cacatcgcgc gccattttgt tatcgacgct gctgtccgtc
     2101 ttcaccgtct cggtattggt cgcattttta aacggcggcg cttaagccag cttgtccata
     2161 aacgaagcca gcgccacgtc gcgctgggta acgccgcctg cgtcgtgcgt ggccaaagta
     2221 acgcttactt tgttgtaaac attagaccat tcaggatgat ggttcagctt ttccgcttgc
     2281 agcgcgaccc tggccataaa cgcaaacgcc tcgccaaaat ccttgaaaac gtattcttta
     2341 tgaatggcgt cgcgccccgc gacggccgtc cagcccttca gcagcggcag ggccgacgtt
     2401 gccccgatca attcgagatt cgccttcatg acaactcctt tttcgacgtg ataccgtact
     2461 ttaaatacta actcctttga ctttgagtgc ggttgagtac tcttacagta cagtccataa
     2521 tagggacgac gcgctcgcgt ggccgtttgc gcctgtttgc aacgcccgca gcccaaataa
     2581 cagtctaccc gttccgccgt gcagtgagtg gttcaagcca ccgccgcaca taagcgaacg
     2641 cgcgcttttt gtatcccttg ccaacctaca cgaggatctg tcatgacaga aagaactcaa
     2701 tgcaagcgac tgcaagtcgc taacgtactc caccagttta ttgaaaacga agccctgccc
     2761 ggcacgggcg tcgcatcggc cgcgttctgg tcggggtttg acgagctcat gcacgatctg
     2821 gcgccgaaaa accgcgcctt gctggccgaa cgcgaccgcc tgcaaaccga gatcaacaat
     2881 tggcatcaca gaaatccagg gcgcataacg gatcagcatg cctatcaaga tttcctgaaa
     2941 gaaatcggct atctcaagcc cgttcccgcc catgttgccg tcaatccgct taatgtcgac
     3001 gtcgaaatca cggaacaggc aggaccgcag ttggtggtgc ccatcaccaa tgcacgctac
     3061 gcgctcaatg ccgccaatgc gcgctggggc agcctgtacg acgcgctcta cggaacggac
     3121 gccatatctg aagaaggcgg cgctacccgc gccggcggct acaatcccgt gcgcggcgca
     3181 aaggtcattg ccttcgcgcg ccattttctg gacgagaacg cgcctttggc gcaaggctca
     3241 cattccgacg cgaccgctta caccgtcagc aacggccaac tgaaagtaac gcttaaaaac
     3301 ggcgcttcat ccgggttaaa gcacgctgaa ggcttcgtcg gctatcgcgg caacgcatca
     3361 agccccgaag ccatcgtgct caagcacaac ggtctgcatt tcgaaattca aatcgataaa
     3421 acaaacccca tcggcaaaca ggactcggcg ggcgtcaaag acatactgat ggaagcggcg
     3481 ctaaccacca ttatggatct ggaagattcc atcgcggccg tcgacgccga cgacaaagta
     3541 ctggcgtatc ggaactggct tggcatcctg aagggcgacc tgacagaaga agtcagcaag
     3601 ggaggcaaaa ctttcacgcg cgccctgaac ccggatcgcc
//
LOCUS       DATCXU010000403         6426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_195053,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000403 DATCXU010000000
VERSION     DATCXU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195053"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="gatB"
                     /locus_tag="VIR04_09055"
     CDS             <1..1011
                     /gene="gatB"
                     /locus_tag="VIR04_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY9460936.1"
                     /translation="NRAGTPLLEIVSEPEMRSAAEAVAYARTLHSLVVWLGICDGNMQ
                     EGSFRFDANVSVRPKGQVEFGTRSECKNLNSFRYLERAISYEVQRQIELIEDGGTVLQ
                     ETRLYDSDRDETRSMRSKEDADDYRYFPDPDLPPLIIAQEWIERVKSTMPELPAHKRS
                     RFEKELGLTGYDAAQLTMDRAVSSYFEELAARLPKGQAKLAANWVLGEVSAALNQNEL
                     EIGQSPVPAQQLASLIGRIIDGTVSNKMARDVFTAMWSGENGGDPDAIIAARGLKQIS
                     DSDAINAMIDQVLAAQPAIVQDYHAGKQKAFNSLVGQVMKAAKGKANPQQVNELLKQK
                     LS"
     gene            complement(1008..1682)
                     /gene="pyrE"
                     /locus_tag="VIR04_09060"
     CDS             complement(1008..1682)
                     /gene="pyrE"
                     /locus_tag="VIR04_09060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY9460937.1"
                     /translation="MADSVRTRTDFVRFSLSCGVLRFGQFRVKSGRLSPYFFNAGLFN
                     SGLSVGRLGAFYAQALVDSNVGFDMLFGPAYKGIPLATATAVALANHPAMAGRDVPFA
                     FNRKEAKDHGEGGRLVGAPLQGKVVIIDDVITAGTSVRESVELIRQAGAQPVAVLIAL
                     DRMERAGAVGTLSEDSAVREVARVYGIPVISIASLDDIMALLSDDDQLAAHREAVAAY
                     RDKYGV"
     gene            1793..3181
                     /gene="dbpA"
                     /locus_tag="VIR04_09065"
     CDS             1793..3181
                     /gene="dbpA"
                     /locus_tag="VIR04_09065"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY9460938.1"
                     /translation="MTPNSFSGLPLTPTLLDNLNSLGYEEMTPIQAQSLPSILAGRDL
                     IARAKTGSGKTAAFGLGILQKLNPDIFSTQALVLCPTRELAEQVANEMRRLARAIGNV
                     KVLTVCGGTPIKPQIESLRSGAPILVGTPGRLLDHIGRGSVDLSAIGILVLDEADRMI
                     DMGFHDDIVNVVKTCPAKRQTLLFSATYPENIRKSSARFLNRPLEVKLELNHDASEIE
                     QHFYEIAPESRNAAVIQLLAHFRPVSTLAFCNTKAQCELLANDLYSHGFSTLALHGDL
                     DQREREDVLVQFSNQSCSVLVATDVAARGLDIQALDAVINVDVTPDAEMYIHRIGRTG
                     RRHEKGLALSLCAPNEMRWASLIEQYQGQKLNWSKLASLRPSPGGRLRAPMVTLRIHG
                     GKKDKLRPGDLLGALTGQAGLNADQVGKINILNSVSFVALERKIAKEALARLSNGAIK
                     NRRFKMGFMPGN"
     gene            3280..3660
                     /locus_tag="VIR04_09070"
     CDS             3280..3660
                     /locus_tag="VIR04_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY9460939.1"
                     /translation="MTTYYSSWSPRVLSIVRIVSGYLFFLHGLAKISGANFQLMSLMG
                     LATAIELVAGALIILGLFTRIAAFFASGEMAVAYFIAHAAKGAVLMPIRNGGDAAVLF
                     CFVFLYLVFAGPGPWSIDQLRRKA"
     gene            3703..4503
                     /locus_tag="VIR04_09075"
     CDS             3703..4503
                     /locus_tag="VIR04_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY9460940.1"
                     /translation="MARIITANLNGIRSAVNKGLLPWIEAQNADFVCFQELKAQASNM
                     TEEMLNPPGLYGYYHYAEKKGYSGVGICARKQPQQVIEGLGVPEIDAEGRYLELVYDG
                     LSVISVYLPSGSSGDLRQTAKFAFMEYFFDHLAQLAQCGRQVILCGDWNIAHKEIDLK
                     NWKGNLKNSGFLPEERAWLTKVFDEQGWVDVYRRLHPETTGDAYTWWSNRGQAWAKNV
                     GWRIDYQIATSKTALSARSASIYKAQRFSDHAPLTIDYDYDVVTHDSP"
     gene            complement(4542..5267)
                     /locus_tag="VIR04_09080"
     CDS             complement(4542..5267)
                     /locus_tag="VIR04_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460941.1"
                     /translation="MSFRCSACLVLSMVIGLTGCAGLGNAPEAAHPSNEEMSMDQFAQ
                     TDFNRTVTIAMRDNLDSLTRLLEKLYRRNPLEWRKTGALDLASAIKRGRKAIETGKAP
                     SELGGLHDVEILSVALDPTYRGDRVGAFIFGLANMIIQAHNGKTRFYVADVLNAQHVY
                     NAARNVEIAAWLLASRRDAAGRPLLLANEISVDASNLSFEREFGQLVGRLDLVANLLD
                     ENLRRVGINYVQGLLFFNFLPVR"
     gene            complement(5340..>6426)
                     /locus_tag="VIR04_09085"
     CDS             complement(5340..>6426)
                     /locus_tag="VIR04_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9460942.1"
                     /translation="LIGSAYTLKFFWAPLVDRYAPPILGRRRSWMFFTQILLAAAIAG
                     MGLISPSTNLSILAFVAVFVAFLSATQDIAFDAYSTDVLHAPERAAGAAIKVLGYRLA
                     MIASGGLALVLADQWLGWTNMYMLMGVMMGLCAIATLLAPEPDVVARPPRSLAVAVVE
                     PLVEFFRRPGAITILLLIVLYKLGDAFAGALSTTFLLRGVGFSVSEVGTVNKVFGLVA
                     TIIGALAGGSLMARLGLYRSLMLFGIFQAISNFGYWVLAVTPHHLYSMTVVVAVENLC
                     GGLGTAAFVALLMALCKQEFSATQFALLSALSAVGRTYLAGPLTPPLVLHLGWPVFFV
                     ITVFIGLPGLILLRMRRTEIQALEAVS"
BASE COUNT         1575 a         1881 c         1728 g         1242 t
ORIGIN      
        1 aaccgcgccg gcacgccctt gctggaaatt gtgtccgagc cggaaatgcg atcggccgct
       61 gaggcggtgg cgtatgcccg caccttgcac agcctggtcg tctggcttgg catctgcgac
      121 ggcaacatgc aagaggggtc gttccgcttc gacgccaatg tttcggtacg ccccaaaggg
      181 caggtcgaat tcggcacccg cagcgaatgt aaaaacctga actcattccg ctatctcgaa
      241 cgcgcgatct cgtacgaagt gcaacgccag atcgagctga ttgaagacgg cggaacagtg
      301 ttgcaggaaa cgcgtttgta tgattcggat cgtgacgaaa cgcgcagcat gcgctccaag
      361 gaagacgccg acgattaccg ctattttccc gatcccgact tgcctccgct gatcattgcg
      421 caagaatgga tcgagcgggt caaaagcacc atgcccgaat taccggcgca taagcgctcg
      481 cgcttcgaaa aagaactcgg tttgaccggt tatgacgcgg cccagctcac catggatcgc
      541 gccgtttcgt catatttcga agaacttgcc gcgcgattgc cgaaaggtca ggcgaaactt
      601 gccgccaact gggtcttggg cgaggtttcg gccgcgctga atcaaaacga acttgaaatc
      661 ggccaaagcc ccgttccagc gcaacagtta gcctctttga ttgggcgcat tatagatggc
      721 acggtctcca acaaaatggc gcgcgacgtc tttaccgcga tgtggtcagg cgaaaacggt
      781 ggcgaccccg acgccatcat cgcggcacgc ggcctgaagc aaattagcga tagcgatgcc
      841 atcaatgcca tgattgacca ggtgctggca gcacagccgg ccattgtcca ggattaccat
      901 gccgggaaac aaaaggcatt caactcgctg gtcggacaag taatgaaagc cgccaagggc
      961 aaagccaacc cgcaacaggt caacgaattg cttaaacaaa agctgagtta aacgccatac
     1021 ttgtcacgat aggcagcgac ggcttcgcgg tgcgccgcca gttgatcatc gtcgctcagc
     1081 aacgccatga tgtcgtccag cgacgcaatg ctgattacgg gaatgccata caccctggcc
     1141 acttcccgca cggccgaatc ctccgacaac gtgcccacgg cgccggcgcg ttccatacgg
     1201 tccagcgcaa tcagcacggc aacgggctgg gcgcccgcct ggcgaataag ctcgactgat
     1261 tcgcgcaccg acgtaccggc cgtgatcacg tcatcaatga tcacaacttt gccttgtagt
     1321 ggcgccccta ccagcctgcc tccctcgcca tgatccttgg cttctttgcg gttgaacgca
     1381 aaagggacat cccggcccgc catggccgga tgattggcca gtgccaccgc cgttgctgtc
     1441 gcgagcggaa tacccttgta ggccgggcca aacagcatgt cgaagccgac gttcgaatcc
     1501 accagcgctt gggcatagaa cgcgcccaag cggcctactg acaatcctga attgaacaat
     1561 cccgcattaa aaaaataggg actcagtcgc cccgatttga ccctgaactg gccaaatcgc
     1621 agcactccgc aactcaaaga aaaacgaacg aagtcagtac gggtacgaac ggaatcagcc
     1681 ataatgcagt cagcgaaaat tgaaaacgcc attatcgttc ataaccattc agtccgcctc
     1741 ggccggcccg ctgttacact ggtgttctct ttcgccggcc tatctcttca ttgtgacccc
     1801 aaattccttt tccggccttc ctctgacgcc gaccctgctc gataacctga acagtctggg
     1861 ttacgaggaa atgactccga tacaagccca aagcctgcca tccattctgg ctggccggga
     1921 tctgattgcc cgggcaaaaa caggcagcgg caaaaccgcc gctttcggat tgggcatact
     1981 gcagaagttg aatccggaca ttttttccac gcaagccctc gtgctctgcc caacgcgcga
     2041 gttggccgaa caagtagcca acgaaatgcg ccggctggcg cgcgccatcg gcaatgtgaa
     2101 agttctgacc gtttgcggcg gtacgcccat caagccacaa atcgaatcac tgcgtagcgg
     2161 cgcccccatc ctcgtgggca cgcctggaag actgctcgat cacatcgggc gcggcagcgt
     2221 ggacttatcg gccatcggca ttctggtgct ggacgaagcg gaccgaatga tcgacatggg
     2281 ctttcacgac gatatcgtca atgtcgtgaa aacatgtccg gccaagcggc aaacgcttct
     2341 gttctcggcc acatacccgg aaaacattcg caagtccagt gcgcgattct tgaacagacc
     2401 ccttgaagtc aagctggaac tcaatcacga cgccagcgaa attgaacagc acttttacga
     2461 aattgcgccc gaaagccgca atgcggccgt gattcaattg ctggcgcatt tcaggcccgt
     2521 atccactctg gccttttgca acacgaaagc gcaatgcgag ctgctcgcca acgatctgta
     2581 tagccatggc ttcagcactc tggcgttgca tggcgatctg gaccaacgcg aacgcgaaga
     2641 tgtactggtg cagttttcca accaaagctg ctccgtgctg gtggcgaccg acgtggcggc
     2701 gcgcggactg gatatccagg cgctggacgc cgtcataaat gtggatgtca cgcccgacgc
     2761 cgagatgtac atccatcgca ttggacgcac cggccgcagg cacgaaaaag gattggccct
     2821 gagcctgtgc gccccgaatg aaatgcgctg ggccagcctg attgaacagt atcagggtca
     2881 aaaattgaac tggtcgaaac tggcctcgct caggccctcc cccggcgggc gactgcgagc
     2941 gcccatggtc acactgcgca ttcacggcgg aaaaaaagac aaactccggc ctggagacct
     3001 attgggcgcg ctgacgggac aggcgggcct gaacgcagac caggttggaa agatcaacat
     3061 actcaactct gtaagcttcg ttgcgctgga gcgaaaaata gccaaagagg ccttagccag
     3121 gctaagcaat ggcgccatca agaacagacg attcaaaatg ggcttcatgc caggcaactg
     3181 agacgaactg accatcggta aaacggacct gtatttcttg ttctgattca cctaaaatcg
     3241 gtgagttcat ccttccttga tcatccatgc gaggtcacta tgacgaccta ttactcaagc
     3301 tggagtccac gcgtgttaag catagtacgc atcgttagcg gctatctgtt ttttttgcat
     3361 ggccttgcca aaatttctgg cgccaatttt cagttgatgt ccctgatggg attggccaca
     3421 gctatcgaac ttgtggcggg cgcgttgatc atcctggggt tattcacccg cattgcagcg
     3481 ttcttcgcat ccggcgaaat ggcggtcgcc tactttattg cgcacgcagc taaaggcgcc
     3541 gtgttgatgc caatacgcaa tggcggcgac gcggccgttt tattttgttt tgtttttcta
     3601 taccttgttt ttgcgggtcc gggcccatgg agcatcgatc agttgcgccg caaagcctga
     3661 tttcagattc gaggtcgttt aaccaaggag caacaagaag aaatggcaag gattatcacc
     3721 gccaatctga acgggatccg ctcggcggtc aataaaggcc tgctgccctg gatcgaagcg
     3781 cagaatgcgg attttgtgtg ttttcaggaa ctgaaggccc aggcgtcaaa catgacagag
     3841 gaaatgctca atccaccggg actatacggt tattaccatt atgccgaaaa gaagggttat
     3901 agcggagtcg gcatctgcgc tcgaaaacaa cctcaacaag tcatcgaagg gctgggcgtg
     3961 ccggaaatcg acgccgaagg acgctatctg gaactcgtct atgacgggct atcagtcatt
     4021 tcagtgtacc tgccctccgg gtcaagtggc gatcttcgcc aaaccgccaa gtttgccttt
     4081 atggagtatt tcttcgatca cctggcgcaa ctggcccaat gcggacgaca agtcattttg
     4141 tgcggcgact ggaacatcgc ccacaaggaa atcgatctca aaaactggaa gggcaatctc
     4201 aaaaacagcg gttttcttcc cgaagaacgt gcctggctga ccaaggtatt cgacgagcag
     4261 ggatgggtgg acgtctatcg ccggctgcac ccggaaacca cgggggacgc ttatacgtgg
     4321 tggagcaatc gcggccaggc ctgggcgaaa aacgtgggtt ggcggataga ttaccaaata
     4381 gcgacgtcga agaccgcctt atccgcacgg tcggcgtcta tttacaaagc acagcggttc
     4441 agcgaccatg ctcccctgac gatagattac gattacgatg tcgtgaccca tgactcgcca
     4501 taacagcggc gatcacgggg ccatccacac agtgcacgcg cctagcgcac cggcagaaag
     4561 ttgaaaaaaa gcaggccttg cacatagttg atgccgaccc tgcgaaggtt ttcgtccagc
     4621 aggttggcaa cgagatcaag ccgcccgacc agttgtccga attcccgctc aaagcttaaa
     4681 ttggaagcgt ccacggagat ttcgttagcc aacagcaatg ggcggcctgc cgcgtctcgc
     4741 cggctggcca gcagccacgc cgcaatctct acattgcgtg ccgcgttgta gacgtgctgc
     4801 gcgttaagga catccgctac ataaaagcgg gttttgccgt tatgagcctg gataatcatg
     4861 ttggcaagac cgaaaatgaa tgcgcccacc cggtcgcctc gatacgtcgg atccagtgca
     4921 acagaaagaa tttccacatc gtgcaacccg ccgagttcgg aaggagcctt gccagtttcg
     4981 attgccttgc gtccgcgctt gatggccgac gccagatcca gagcgccagt cttgcgccat
     5041 tcgagaggat tacgccgata cagtttttca agcagacgcg tcagactatc caggttatcg
     5101 cgcatggcga tcgttacggt acgattgaaa tcggtctgcg caaactgatc catcgacatt
     5161 tcttcgttgc ttggatgggc cgcctccggg gcattgccca gcccggcaca gcccgtcagt
     5221 ccaattacca tcgataaaac caggcatgcc gaacaacgaa aactcattgg agttttctcc
     5281 agggaaccct gacgttcagc cagggaggaa ggagtcgatc acggctttat cggcgtagct
     5341 tagcttacgg cctccagggc ctgaatctcg gtgcggcgca tgcgcaacag aatcaggcca
     5401 ggcaatccaa taaaaaccgt gatcacgaaa aataccggcc accccagatg caaaaccagg
     5461 ggcggcgtca aaggtcccgc cagataagtg cggcccacgg ccgacaaggc cgacaacaag
     5521 gcaaattgcg tggccgagaa ttcctgtttg caaagcgcca tcagcagcgc cacaaaggcg
     5581 gccgtgccca gccctccgca caaattttcc acagccacca cgacggtcat cgaatagaga
     5641 tggtgcggcg tcaccgcgag cacccaataa ccgaaattgg aaatcgcctg gaaaatgcca
     5701 aataacatca aggatcgata caggccaagg cgagccatca aagacccgcc agccaatgca
     5761 ccgatgatcg tcgccaccag cccgaacacc ttgtttaccg tacccacttc ggaaacactg
     5821 aaacccactc cgcgcagcaa gaaggttgtc gatagtgcgc ccgcaaaggc gtcgcccagc
     5881 ttgtacagaa cgatcagcaa caagatcgtg atggcgccgg ggcgcctgaa aaattcgact
     5941 aaaggctcaa cgaccgcaac cgccagcgac cgtggcggcc gcgccaccac atcaggttcg
     6001 ggcgccagta acgtcgcaat cgcacacaac cccatcatta cgcccatgag catatacatg
     6061 ttggtccacc ccagccactg atcagccagt accagggcca ggccgcccga tgcgatcatt
     6121 gccaggcgat aacccagcac cttgatcgca gcgcccgccg cgcgttcggg cgcgtgcagc
     6181 acatcagtgc tgtaagcatc gaatgcaata tcttgcgtgg cggacaaaaa cgccacgaaa
     6241 acggccacga acgcaagaat ggacaagttc gtcgaaggcg aaatcaggcc catgcccgcg
     6301 atagcggcgg ccaggagaat ttgcgtgaaa aacatccagc ttcggcggcg acccaggatt
     6361 gggggagcat atcgatcgac gagtggcgcc caaaaaaatt tcagtgtgta ggccgagcct
     6421 atcagc
//
LOCUS       DATCXU010000404         8129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1059755,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000404 DATCXU010000000
VERSION     DATCXU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059755"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VIR04_09090"
     CDS             complement(<1..1144)
                     /locus_tag="VIR04_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEY9460943.1"
                     /translation="MYKESDKIVFSPSDLTTFMSSPFASWMNRWKLECPEDVPAIDEA
                     DELDKTLAEKGLTHEAALLDEFRAQGLKVVDIAAGAQNANSHYAHRYRATREAMRDGV
                     DVIYQALLEKEPFRGHADFLIKAPGASDLGDYHYEVWDTKLASSVKPYFVVQLCCYAE
                     MLEHMQGRRPAHIVVALGNKKRERLETDDYFYYYLALRAQFLQAQDAFDVHAMPDPAD
                     SKSFGRWSDYAGRMLQESDHLSLIANITRSQIKRLNRAGIRTCQQLITRNAEHISGIT
                     AELLAKLTAQASIQQASQGKDRPLFDVLPPASEPKQIGLALLPPHNPLDVFFDIEGFP
                     LDEGGLEYLWGCTYFDGSGIRQFKDFWAHDREQEKQAFGSFIAWVYA"
     gene            1329..3389
                     /locus_tag="VIR04_09095"
     CDS             1329..3389
                     /locus_tag="VIR04_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY9460944.1"
                     /translation="MLEVPPLIRLWVLRLLVDLNGQKEFVDRDSFSSDKLAEFLGLGK
                     WINPDDGEFSAKLIKAELRKLRQAAELDASYHEIPSCLKKNVQRLAKLSGMTEVDCRI
                     LEFAVLLKSERLLEDATDILGQLSSSKAYFALSTLLNLPPGAVSTALSENGILCRTGL
                     VSIERSGKYELSEKFNLISNGFADRVCSSDTDPINLLRDMVVLSEPPELSLDDYGHVA
                     KSLAVLQLYLKHSIATRRKGVNIFLHGLPGTGKTQLSKVLAKELSCDLFEITSEDDDG
                     DAISGESRLRAYRATQGFFGGRKVLVMFDEAEDVFNDGNFDFGRKSTAQLHKSWINRM
                     LEENILPTLWCSNSVRCLDPAFVRRFDMVIELPIPPRQQREKILQANCADLLDTAAIS
                     RMAESEQLAPAIAARAARVIRPIHLELGKEGAQSAMELMVNCTLEAQGHKTIQREGDP
                     NRLPDIYDPTFINADANLEEVAKGLAQTGAGRLCLYGPPGTGKTAYGRWLAGQLQRPL
                     AIRRASDLISMWVGETEKNIADAFKRAEQDHAVLLIDEVDSFLQDRRGASQSWEVTAV
                     NEMLTQMESFPGVFVASTNLMNGLDQAALRRFDLKVKFNFLTDDQAYELVCRHCAMLN
                     IPPPGAATRARLGQINTLSPGDFAAVIRQHRFRPITSARAMVIALAAECSLKEEDKGH
                     IGFI"
     gene            3421..4473
                     /locus_tag="VIR04_09100"
     CDS             3421..4473
                     /locus_tag="VIR04_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY9460945.1"
                     /translation="MQAQPPEIAQSYLNAMSARPKNIETVALTLELLKRIPRNQKITI
                     AELHRQLRDMGIDRTVRTIERQIDALVQHFDIECDDRSKPFGYRWKANAKGLSLPNLN
                     GPESLLLALAQRHLQNLLPASVMKSMEGFFAQAKANLDPYSSKSREREWLDKVRVVDA
                     TQPLLPPTIKADVLEAVTSALYQNLWLTVDYKNARGTVATSNIMPLGLAQQGPRLYLV
                     CRYEGFDNERSLAVHRIKSAAVSGLSFTRPPEFSLQKYDDDGRFGFGEGETVALSFDI
                     EKKVGRHLLETPLAKDQVVTEHEDYYHISATVIDSKRLDQWLNGFARDVWGVDKQKKV
                     KTQVVIAAHSAQNQCQ"
     gene            4625..5296
                     /locus_tag="VIR04_09105"
     CDS             4625..5296
                     /locus_tag="VIR04_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEY9460946.1"
                     /translation="MLRLRYLPLLAAFAMPLFYTAPSQGETIIDEWQNVKAPPPPALK
                     SVTLDPKTTALLMIDIIKQTCNMKMRPRCVAMVPKVQKLLTEARDNGVYVIYALFPSP
                     KPATFPNPKISDYVPELAAKGTEPVVIAFVDKFMLGDRDTGLQKLLKDKGIKTLIPVG
                     VSSHNGVLFTSVMASLRGFDVVVPVDGMAGNNAYEDQATDYILTSSIVYKVTLTSIDQ
                     IKFEK"
     gene            5524..6252
                     /locus_tag="VIR04_09110"
     CDS             5524..6252
                     /locus_tag="VIR04_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9460947.1"
                     /translation="MIEFKNVNKWFGDLHVLQDINLRAEKGEVLVICGPSGSGKSTLI
                     RCANGLEEVQKGTILINDLCVTDPLVKRSLISTEVGMVFQQFNLYPHMTVLKNITLAP
                     LKVRNESTRVAEQYARELLEKVHIPMHANKYPSQLSGGQQQRVAIARAMAMRPKAILF
                     DEPTSALDPEMVNEVLEVMTELAADGMTMIVVTHEMGFARRVADRVIFMDAGIIIEEG
                     APESFFQQPKNERTKAFLSKILTH"
     gene            6307..7122
                     /locus_tag="VIR04_09115"
     CDS             6307..7122
                     /locus_tag="VIR04_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9460948.1"
                     /translation="MHLKKPNHLLRGLLLLLPLVFVLAGTGSVSYADTLSTIKERGEL
                     IAGVKAQYPPFGFVDSSGKNAGFDVDLSRELARGLFGNPDKVKFVTVTSGNRVPYLQS
                     KKIDIIAATVTVTDERKKTVDFSDPYFNSGTLILVPKSSSITGINDLAGKKVAVIQGS
                     IQVEDLADLAPKATLIKFGKTSEAVLAVKTGRADAYAHDDIAILDIAHGDPGLKVVGE
                     DYRPRPFAMAVRKDDAEFLKFVNDRIREMKKDGTFDKLWNRWFGDYKKLVKPQ"
     gene            7142..7792
                     /locus_tag="VIR04_09120"
     CDS             7142..7792
                     /locus_tag="VIR04_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9460949.1"
                     /translation="MTLDVLAHLLPLLAMGAGLTILVSAAAMVLAIVLGMVTAVLSQM
                     PGKWIRRLVRGYVELFRNTPLLIQLFILYFGFPQIGISMSPLTCGILALAIYTGAYNV
                     EIFRAGLEAVPIGQHEAAVATGLSRFQEAFYVIIPQALRISFPSLGNNLVSLVKNSSL
                     VSTIGLADLMFQANEAAYTDFLSFPAYGLAVVLYVVIILLLTRVINRIDRTLTARQ"
     gene            7798..>8129
                     /locus_tag="VIR04_09125"
     CDS             7798..>8129
                     /locus_tag="VIR04_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9460950.1"
                     /translation="MGPYIEWSDYLLKGALVTIQIALASIAIGTIAGFLVGLLRFVPS
                     PSVRSTVAWTVEIIRAVPLLLMLFFIFFALPVIGFDIPIGLSATLAMSLWMTVNTAEV
                     VRGGIQSIS"
BASE COUNT         1887 a         2133 c         2247 g         1862 t
ORIGIN      
        1 gcgcataaac ccacgcgatg aatgaaccaa aggcctgctt ttcctgctcg cggtcatggg
       61 cccagaaatc cttgaattga cgaatcccgg agccgtcaaa gtaggtgcat ccccataggt
      121 attccaggcc gccctcgtcc agcggaaagc cttcaatgtc aaagaacaca tccagcggat
      181 tatgcggtgg caataaagcc aggccgatct gctttggttc gcttgccggc ggcaacacgt
      241 cgaacaacgg gcggtccttg ccctggcttg cctgctgaat gctggcctgg gcggtcagct
      301 tggcaagcaa ttcggcagtg atgccggata tatgttctgc attgcgtgta atcaattgct
      361 ggcatgtgcg gataccggca cggttcagcc tttttatttg cgaacgggta atgttggcga
      421 tcaacgaaag gtggtcactt tcttgcagca ttcgcccggc atagtcagac cagcgcccga
      481 aacttttcga gtccgccggg tcgggcattg cgtggacatc gaatgcgtct tgcgcctgca
      541 ggaattgcgc gcgtagcgcc aggtagtaat aaaaatagtc gtcggtttca agcctttctc
      601 gcttcttgtt gcccaaggcc acgacaatgt gtgcggggcg gcgtccctgc atgtgttcca
      661 gcatctctgc gtaacagcaa agctgcacca cgaaataggg ttttaccgac gatgcaagct
      721 tggtatccca cacttcgtag tgataatcgc cgaggtcgct ggcgcctggc gccttgatca
      781 ggaagtcggc gtggccgcga aacggctcct tttcaagcag cgcttgataa atcacgtcaa
      841 caccgtcccg catggcctcg cgcgttgcac gatacctgtg cgcgtaatgg ctgttggcgt
      901 tttgggcgcc ggccgcaatg tccacaacct tcaggccctg cgccctgaat tcatccaata
      961 gtgcggcttc atgggtcagc cccttttcgg cgagcgtctt gtccagttca tccgcttcat
     1021 caatggccgg aacatcttca gggcattcca gcttccagcg gttcatccat gacgcaaaag
     1081 ggctgctcat gaacgtggtg aggtcacttg gcgaaaatac gattttgtct gactctttgt
     1141 acacgtatta gtccttgatc ctgggggcgc ggcgatccat agatatcctg accacctgtg
     1201 tttattttag gccgaaattt gatggctcca tgcgacgcgt tttgacgtat cagactggat
     1261 aattatctct gtgcttcgag gagattatca tcatcaggtt acgcaaatta ttgggcagtc
     1321 ccgcgtctat gcttgaagtt ccgccgctaa tccggttatg ggttctgcgg ttgctggttg
     1381 atctgaatgg ccagaaggaa ttcgttgatc gcgattcctt ttccagtgac aagctggccg
     1441 agtttctggg cctgggcaag tggattaacc cagacgatgg cgagttcagc gcaaaactga
     1501 taaaggccga gctgcgcaaa ctccggcagg cggcagaact ggacgcaagc tatcacgaaa
     1561 tcccttcctg cttaaagaaa aacgttcagc ggctggcaaa actaagtggc atgacagaag
     1621 tggactgccg aattctggaa ttcgctgttc ttttaaaaag cgagcgactg cttgaagatg
     1681 cgacagacat cttggggcaa ctaagttcca gcaaagcata ctttgccctg tcgacattat
     1741 tgaaccttcc gccaggcgca gtaagcactg cgttaagcga gaacggcatt ctttgcagaa
     1801 cagggctggt ttcgatcgag cgctcgggca agtacgaatt aagcgagaag ttcaacctga
     1861 tctcgaacgg attcgccgac cgtgtttgtt catctgacac ggatcccatt aaccttctgc
     1921 gtgacatggt tgtattgagc gagccgcccg agctttcgct cgacgattac ggccacgtcg
     1981 caaagtcgct ggctgtgctg cagctttatc tcaagcattc gatcgccacg cgtcgcaaag
     2041 gggtaaacat atttttgcat ggtctgcccg gcaccggaaa aacccagttg tccaaagtgc
     2101 ttgccaagga gctttcctgc gacttgttcg aaattaccag cgaagacgat gacggcgacg
     2161 ccattagtgg tgaatcccgg ttgcgggcct atcgtgcgac ccaaggcttc tttggcgggc
     2221 gcaaggtgct ggttatgttc gacgaagcgg aagacgtctt caatgacggc aactttgatt
     2281 ttgggcgaaa aagcacggca caattgcata agtcctggat caaccgcatg ctcgaagaaa
     2341 atatcctccc gaccttgtgg tgttccaata gcgtgcgttg cctggacccg gccttcgttc
     2401 ggcgcttcga catggtgatc gagctgccca ttccgcccag gcaacaacgc gaaaaaattc
     2461 tgcaggccaa ttgcgccgat ctgctcgaca ctgcagccat ttcaaggatg gctgaatccg
     2521 agcaactggc gcccgcgatt gcggcgcgcg ccgcccgcgt gattcggccc attcatttgg
     2581 aactggggaa ggaaggcgcg cagtcagcca tggagttgat ggtcaactgt acgttggagg
     2641 ctcagggcca taaaacgata cagcgcgagg gcgacccgaa ccggcttccc gatatctacg
     2701 atccaacgtt tattaatgcc gacgccaact tggaagaagt ggccaaaggc ctggctcaaa
     2761 ccggtgcggg tcgcttgtgc ctgtacggcc cgccgggtac agggaaaacc gcctatggcc
     2821 gctggctggc cggacaactg caacgccccc tggccattcg acgggcgtcg gatctgatat
     2881 cgatgtgggt gggggaaacc gaaaaaaata ttgccgatgc gttcaagcgg gccgagcagg
     2941 atcacgctgt attgctgatc gatgaggtgg acagctttct gcaggaccgc cggggcgcca
     3001 gccaaagctg ggaggtgact gcggtgaatg agatgctgac ccaaatggaa tcgtttccgg
     3061 gggtcttcgt cgcttcgacc aatttgatga atggtctgga tcaggcggca ctgcgccgtt
     3121 tcgatctgaa ggtaaagttc aatttcctga cagacgatca ggcttatgaa ttggtatgcc
     3181 gacattgcgc aatgttaaac ataccgccac cgggtgcagc aacccgggcg cggcttggtc
     3241 agatcaatac actgtcccca ggcgatttcg cggctgtcat tcgtcagcat cggtttcgcc
     3301 cgattacgtc cgcgcgtgcc atggtgatcg cgctggccgc cgagtgcagt cttaaagaag
     3361 aagacaaagg ccatatcggt tttatttaat agatatggat tgctggcata gcgcgctatt
     3421 atgcaggccc aaccaccgga aatcgcccag tcttacctga acgccatgtc tgcccgaccc
     3481 aagaatatag aaaccgtagc gctaacactg gaactgctaa agcgtattcc gcgcaaccaa
     3541 aagataacga ttgccgaact gcaccggcaa ttgcgcgata tgggaataga ccgtacggta
     3601 cggaccatag aacgccagat cgacgcattg gtccaacact ttgatatcga atgcgacgat
     3661 agaagcaagc catttgggta tcgatggaaa gcgaacgcca aaggcttgtc gcttcccaac
     3721 ctgaatggcc cggagtcact gctactggcc ctggcccaac ggcatttgca aaacctgttg
     3781 ccggctagcg tgatgaaatc gatggaaggc ttctttgccc aggcgaaagc caacctggat
     3841 ccctattcca gcaagagccg tgaaagagag tggctggaca aagtgcgggt ggtggacgcc
     3901 acgcaacctc tgttgcctcc aaccataaaa gccgacgtac tcgaagccgt aactagcgct
     3961 ctgtatcaga acctgtggct tacggtggac tacaaaaacg ccagaggcac ggtcgctacg
     4021 agcaacataa tgccattggg gttggcgcaa caggggccaa ggttgtacct ggtgtgtcgc
     4081 tatgaaggct ttgacaatga gcgtagcctg gcggtccatc gcattaaaag cgcggctgta
     4141 tcaggactga gctttacacg gccacccgaa ttcagtctgc aaaaatatga cgacgatggg
     4201 cgcttcgggt ttggcgaagg agagactgtc gcgctttcgt tcgatattga aaaaaaagtg
     4261 ggccggcatc tgctggaaac gccgctggcc aaagatcagg tcgttacgga gcatgaagat
     4321 tattaccaca tcagcgcgac agttatcgac tccaagcgcc tggatcagtg gctgaacggg
     4381 tttgcgcggg acgtttgggg tgttgataaa cagaagaaag ttaaaacgca agttgtgatc
     4441 gcagcccaca gcgcgcagaa ccaatgccaa tgagatgtcc gcgtccagcc gattagctct
     4501 accctaatgg cagaactcgc cccaaaatgg gaattgcggc gccagaaaga atgcagtata
     4561 tctatccggc tttttccgga attcatctgt tagttctatt cgacgaagaa actgggagga
     4621 caacatgttg cgcttacgat atctgccgct gcttgccgct ttcgcgatgc ccctgtttta
     4681 cactgcgcca tctcagggcg agacaatcat tgacgaatgg caaaatgtga aggcgccgcc
     4741 accgcccgcg ctcaaatcgg tcacgcttga cccgaagacg acggcgctcc tgatgatcga
     4801 catcatcaag cagacgtgca acatgaaaat gcgtccgcgt tgtgtggcca tggttcccaa
     4861 agttcagaag ctgttgaccg aggcgcgcga caatggcgtg tatgtaatct acgccttgtt
     4921 ccccagcccg aaaccggcga cctttcccaa tccgaagatc agcgattacg ttccggagct
     4981 tgcggcgaaa ggcaccgagc ccgtggtgat agcatttgtc gacaagttca tgcttggcga
     5041 cagagatacc ggccttcaaa agctgctgaa ggataagggg attaaaaccc tcattccagt
     5101 tggcgtttcg tcgcacaatg gtgtcctgtt cacgtcggtc atggcttctt tgcgcgggtt
     5161 tgacgtggtt gtcccggtgg atggcatggc gggaaataac gcctatgaag atcaggccac
     5221 ggactatatt ttgacgagtt cgattgtata caaggtcacg cttaccagca ttgatcagat
     5281 caaattcgaa aagtaatcgc gggctaaaac aagctgaatc aggcaccgcc gcatggcggc
     5341 cttgcttcgt gtgccgcctg cgcaaatcat gtgctaatag ggtaaccgct aatatcggat
     5401 tcccttattt atgcataata attagccctt ttattcgaac cgtcatgcat cggaatcgcg
     5461 ctacggcaac cagccgaatc ttggcgcgtg ggcgatgtct cccgccgata aacatataca
     5521 gtcatgatag agttcaaaaa cgtaaataag tggttcgggg atcttcacgt tttacaagac
     5581 ataaacctgc gtgctgaaaa aggcgaggtg ctggttatct gcggaccaag cggatcgggg
     5641 aaaagcacgc tcattcgttg tgccaatggt ctggaggagg tgcaaaaggg cacaattctg
     5701 atcaatgact tatgcgttac ggatccgctt gtgaagcgca gtctcatcag tacggaagtt
     5761 ggcatggtct tccagcaatt caatctttac cctcacatga ccgtcctcaa aaatattaca
     5821 ttggcgccat tgaaggtccg gaatgaatcc acccgcgtag cggagcaata tgcgcgtgaa
     5881 ctcctggaaa aagtgcacat acccatgcat gccaacaaat atccctcgca gctatccggc
     5941 gggcagcagc aacgcgtagc cattgcgcga gccatggcca tgcgtccgaa agcgattttg
     6001 ttcgatgaac ccacctctgc ccttgatccc gagatggtga atgaagtgct tgaggtgatg
     6061 actgagctgg ccgccgatgg tatgacaatg atagtagtca cccacgagat gggattcgcg
     6121 cgacgcgtgg cggatcgggt catattcatg gatgccggta tcattatcga ggaaggggcg
     6181 ccagagagct ttttccaaca acccaaaaat gaacgaacca aggcgttttt gagcaagatc
     6241 cttactcatt aaggcgctgg ggcttatcag gcatcaaata tcaatccaac tatcggggac
     6301 aaaatcatgc atctaaagaa accaaatcac cttcttcgag gcttgttgct tcttttgcct
     6361 ctcgtgttcg tccttgcagg cacgggatca gtctcctatg ccgacacatt aagcaccatc
     6421 aaggaacgtg gagagctcat agccggcgta aaagcgcaat acccgccgtt tggattcgtc
     6481 gatagttcgg gaaagaacgc gggatttgac gttgatctgt cgcgcgagct ggcgcgtggt
     6541 ctgtttggaa atcccgacaa ggtaaagttc gtcacggtga cttccggaaa tagggtgccg
     6601 tatctgcaga gcaagaagat agacatcatc gcggcgaccg tcacggtaac ggacgaacgg
     6661 aagaagactg tggacttctc tgatccgtac ttcaattcag gcactttgat tcttgtgccg
     6721 aagtccagct ccatcaccgg gatcaacgat ctcgctggaa agaaggtggc cgtcatacag
     6781 ggttccattc aggtcgagga ccttgccgac ttggcgccga aggcgacgct gataaagttc
     6841 ggaaaaacct ccgaggccgt tctggctgtg aagacgggcc gtgccgatgc ctacgcacat
     6901 gatgacatcg ccatactgga tatcgcgcat ggggatccag ggctgaaagt cgttggagag
     6961 gactaccggc cacgcccatt cgccatggcg gttcgcaagg atgacgcgga gttccttaaa
     7021 ttcgtcaacg accggatccg cgaaatgaag aaagatggga cattcgacaa attgtggaac
     7081 cgctggtttg gcgattacaa aaagctggta aagccacagt gattggcgga tatatcgcgc
     7141 aatgactctc gacgtgctgg cccacctgct cccgttgctg gcgatggggg cgggactgac
     7201 gatactggtg tccgcggcgg caatggtgct ggccattgtg cttggcatgg tgaccgccgt
     7261 actttcacag atgccgggca aatggatccg cagactcgtg cgcgggtatg tggagctttt
     7321 caggaatact ccgctgttga tacagctatt tattttgtat ttcgggtttc cacagatcgg
     7381 gatcagcatg tcgccgctca cgtgcggcat cctggccttg gcaatctata ccggcgctta
     7441 taacgtcgaa atatttcgcg ctggtctgga ggctgtgccg atcggtcagc acgaggccgc
     7501 cgtcgccacg ggactgtcac gcttccagga ggcgttctat gtgattatcc cgcaggctct
     7561 ccgaatttct ttcccttccc tgggcaataa cctggtatcg ctcgtcaaga attcatcatt
     7621 ggtctccacc atcgggctcg ccgacctcat gtttcaggcc aacgaggccg cctatacgga
     7681 cttcctgagt ttccccgcct atgggctggc cgtggtgctc tatgtggtga tcatattgct
     7741 gctgacacgt gtgattaaca gaatagaccg aaccctgaca gcgcggcaat gatgaatatg
     7801 ggtccttaca ttgagtggag cgattacctt ctgaagggag cgctcgtcac catccagatc
     7861 gcgctggcgt ccatcgccat cgggacgatc gcaggattcc tggtcggctt gttgcgcttc
     7921 gtaccctcgc catcggtccg atcgacagtc gcctggaccg tcgaaattat tcgcgccgtt
     7981 ccgctcttgc tgatgctgtt cttcatattt ttcgcactgc cggtaattgg ttttgacata
     8041 ccgatcgggc tttccgccac cttggccatg tcgctctgga tgaccgtgaa taccgccgaa
     8101 gtcgtgagag gcgggatcca gtcgatttc
//
LOCUS       DATCXU010000405         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_907441,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000405 DATCXU010000000
VERSION     DATCXU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907441"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1462
                     /gene="miaB"
                     /locus_tag="VIR04_09130"
     CDS             62..1462
                     /gene="miaB"
                     /locus_tag="VIR04_09130"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY9460951.1"
                     /translation="MQEIPSRIEPDAEPAKKRKLFIRTFGCQMNEYDSSKMADILHQT
                     QGLELTQDPEEADVILFNTCSVREKAQEKVFSDLGRVRHLKQNNPDLIIGVGGCVASQ
                     EGAAIVERAPFVDLVFGPQTLHRLPQLIAKRKETGRPQVDISFPEIEKFDALPPARVE
                     GPTAFVSIMEGCSKYCSFCVVPYTRGPEVSRPFDDVLVEVADLADQGVKEVTLLGQNV
                     NAYRGPMGESAEIADFAMLLEYVHEIPGIERIRYTTSHPKEMTTRLIEAHGALPKLVP
                     FLHLPVQAGSDRILGRMKRGYTTLEYKSIVRRLRAARPGLGLSSDFIVGFPGESEVDF
                     EKTMSLIRDVGFDQSYSFLYSRRPGTPASDMRDDTPKELKLERLHRLQALVNEQANAI
                     AHAMIGTTQRILVERPSRRDPNELSGRTENNRIVNFTGQPRLIGQMVDVRITQTNTNS
                     LKGEVVISGSTLEGHA"
     gene            1459..2469
                     /locus_tag="VIR04_09135"
     CDS             1459..2469
                     /locus_tag="VIR04_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY9460952.1"
                     /translation="MNHAPRHTPPVVITLEGDNTNLANLCGPLDANLRQIADNWNVKL
                     NRRGGRISIDGEHAQAVGKALKWFHRRAVHQALSVDDIQLGLVELDAQRESRVKEAVN
                     VQPVPEPPPSDGGRVMLRTPKGDVRPRTPRQRDYIEQILEHDVTFGLGPAGTGKTWLA
                     VACAIDALERETVQRLVLTRPAVEAGERLGFLPGDLVQKVDPYLRPLYDALYDLMGIE
                     RVQRLFDKQTIEVAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGFGSKAVVN
                     GDPSQVDLPRDQASGLNHAVDVLKSVAGIATTRFTSRDVVRHPLVGRIVDAYEQAGDH
                     VG"
     gene            2459..2926
                     /gene="ybeY"
                     /locus_tag="VIR04_09140"
     CDS             2459..2926
                     /gene="ybeY"
                     /locus_tag="VIR04_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY9460953.1"
                     /translation="MSVRLTLAVQYATPAAELPRWRLRRWVRRAVDTLSATASPPFNA
                     ITLTLRLVDAAEGRLLNRKFRQKDYATNVLTFEYGIDADGTAGGDIVLCAPVLRSEAH
                     QQKKTLLEHAAHLTVHGVLHAVGYDHVKPDEAEIMEALEIAILGKMGIENPYR"
     gene            2973..3857
                     /locus_tag="VIR04_09145"
     CDS             2973..3857
                     /locus_tag="VIR04_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY9460954.1"
                     /translation="MPEPSPPGAEPRPARHIQKSLLAKLTSFIRPEPEDREDIKAVLE
                     AAHDRQVLDGESYAMITGALDVANQTVADIMVPRSKMDMLDMGKPLAELLPEIIETGH
                     SRFPVYENHRDNIIGILLAKDLLWSITNPSIALRPLVRPAVFIPETKRLNLLLHDFRS
                     SRNHLAIVIDEHGGTTGLVTMEDVLEQIVGDIEDEYDEEAEKTIFQTGKNNWRVMGIT
                     EIDVFNDELHTNLPHDEYDTLGGWLAAELGLMPRRGDSIVCQGLNITVVGADARRALW
                     LHVQPEERDSSSQSNAAG"
BASE COUNT          914 a         1205 c         1099 g          742 t
ORIGIN      
        1 ttgaaaaaag acgatccccg ggcagagatc agctaaatca cattccgtta ccgcaatcgc
       61 catgcaagaa atacccagcc gcatcgaacc cgacgccgag cccgccaaga agcgaaaact
      121 gttcatacgc acatttggtt gccagatgaa cgagtacgat tccagcaaaa tggccgacat
      181 tttgcatcag acgcaggggc tggaattgac gcaagatcct gaagaagccg acgtcattct
      241 gttcaatacc tgttcagtgc gcgaaaaggc gcaggagaaa gttttttccg accttgggcg
      301 cgtacgccat cttaaacaga acaaccccga cctgattatc ggggtcggcg gctgcgtggc
      361 cagccaggaa ggcgcagcga ttgtcgaacg cgcgcctttc gtcgatttgg tttttggccc
      421 acaaaccttg caccgactgc cgcagctcat cgccaaacga aaagaaaccg gccggcctca
      481 ggtcgacatc agttttccgg aaatcgaaaa attcgacgcc ctgcccccgg cccgggtcga
      541 aggcccaacg gcatttgtct cgatcatgga aggttgcagc aagtactgca gcttctgcgt
      601 cgtgccctat acgcgcggcc ccgaagtgtc gcgtcctttc gacgacgtgc tggtcgaagt
      661 cgccgacctg gccgaccagg gtgtaaaaga agtcaccttg ctgggccaaa acgtgaacgc
      721 ctaccgcggc cccatgggcg aaagcgccga aatcgccgac ttcgccatgc tgctggaata
      781 tgtccacgaa atcccgggga tcgagcgcat acgctacacc acgtcgcatc ccaaggaaat
      841 gaccacacgc cttatcgaag ctcacggcgc gctccccaag cttgtcccct tcctgcattt
      901 gcccgtgcag gccggtagcg accgcattct gggccgcatg aaacgcggct acaccacact
      961 ggaatataag tccatcgtga ggcgcctgcg ggccgcacgg cccggactgg gactgtcctc
     1021 ggactttatc gtgggatttc ccggcgaaag cgaggtggat ttcgagaaaa ccatgtcgct
     1081 gattcgcgat gtggggttcg atcagtccta ttcattcctt tactcgcgcc gcccggggac
     1141 accggcctcc gatatgcgcg acgacacacc gaaagaatta aagctggaac gactgcatcg
     1201 cttgcaagcg ctggtgaacg aacaagccaa cgccatcgct cacgccatga taggcaccac
     1261 acaacgaata ctggttgaaa gaccatcgcg gcgcgacccg aacgaactct cgggccgcac
     1321 tgaaaataac cggatcgtca actttaccgg ccagccccgt ctgattggac aaatggtcga
     1381 tgtgcgcatc acccagacaa acacaaattc gctgaaaggc gaggtcgtaa tttcaggctc
     1441 tacactggaa ggccatgcat gaatcatgcg ccgcgccaca caccgcccgt tgtcataacg
     1501 cttgagggcg acaacaccaa ccttgccaat ctttgcggcc cgctggatgc aaatctgcgc
     1561 cagatcgccg ataactggaa tgtcaagcta aaccggcgcg gcggacgcat atcgattgat
     1621 ggcgaacacg cgcaagccgt gggtaaagcc ctgaaatggt ttcatcgccg cgcggtgcac
     1681 caggcattgt cggtggacga cattcaactg ggattggtgg aactggacgc gcagcgcgaa
     1741 tcccgtgtca aggaagccgt taacgtccaa cccgtgccgg aaccgccgcc cagtgacggc
     1801 ggccgcgtaa tgctgcgcac gccaaaaggc gacgtgcggc cgcgaacgcc gcgccagcgc
     1861 gactatatcg agcaaatcct cgaacatgac gtcaccttcg gccttgggcc ggccggcacc
     1921 ggcaagacgt ggctggccgt ggcgtgcgcc atcgacgcgc ttgaacgcga gacggttcag
     1981 cgtctggtcc tgacccggcc cgccgtcgag gcgggagaac ggctgggctt tctgccgggc
     2041 gacctggtgc aaaaggtgga tccgtatttg cgccccctgt acgatgcgtt gtatgacttg
     2101 atgggaatcg agcgggtgca acgtctgttc gacaaacaga ccatcgaagt cgcgccattg
     2161 gcttacatgc gcggccgaac gctgaatcac gctttcatca ttctggacga ggcgcaaaac
     2221 accacgcccg aacagatgaa aatgtttctc acgcgcatcg gattcggcag caaagccgtt
     2281 gtcaacgggg atccttccca ggttgatttg ccacgcgacc aggccagcgg ccttaaccac
     2341 gccgtggacg tgctcaaatc cgttgcggga attgccacca cccgcttcac cagtcgcgac
     2401 gttgtacgcc atcctttagt ggggcgtatt gtcgacgctt acgagcaggc gggcgaccat
     2461 gtcggttagg ctgaccctgg ccgttcagta cgccacgccc gccgccgaat tgccgcgctg
     2521 gcgcctgcgt cgctgggtcc gccgggcggt cgatactttg tccgctaccg catcgccgcc
     2581 attcaatgcc atcacactga cgttgcgtct ggtcgacgcc gcggaaggcc gtcttttaaa
     2641 tcgcaaattc cgccaaaagg attacgccac aaacgtattg acatttgaat acggcattga
     2701 tgcggacggg accgccggcg gcgacatcgt cctttgcgcg ccggtgctgc gcagcgaggc
     2761 gcaccaacag aaaaagaccc tgctcgaaca tgccgcccat ctaaccgtgc acggcgtgct
     2821 gcacgccgtt ggttacgatc atgtgaaacc ggacgaggcg gaaataatgg aagccctgga
     2881 aatcgccatt ctcggaaaaa tggggatcga aaatccctat cgatagccaa tttcggttat
     2941 gctaaaactc tttacagcca gtccaatgga caatgccaga acctagcccc cccggtgccg
     3001 agcctcgccc ggcacgacac atccagaaat ctctcctggc aaaacttact tcgttcatcc
     3061 ggcccgaacc cgaagatcgg gaagacatca aagccgttct cgaggccgca cacgacaggc
     3121 aggtgcttga cggtgaatcg tacgcgatga ttacgggcgc gctcgatgta gccaaccaga
     3181 ccgttgccga cattatggtg ccgcgttcga aaatggatat gctcgacatg ggcaagcccc
     3241 tggccgagct actgccagaa atcatcgaaa ccgggcactc gcgcttcccc gtctacgaga
     3301 atcatcggga caatatcata ggtattttgc tggccaaaga tctgctctgg tcaatcacca
     3361 atccatcgat cgcgttaaga cctttggtgc gccccgccgt gttcattcct gaaaccaagc
     3421 gcctgaatct gctgctgcac gattttcgca gcagccgcaa ccacctggcg atcgtcattg
     3481 acgaacatgg cggcaccacc ggcctggtga ccatggagga cgtgctcgaa caaatcgtcg
     3541 gcgacatcga agacgagtat gacgaagaag ccgaaaaaac aatttttcaa acgggcaaaa
     3601 ataactggcg cgtgatggga atcactgaaa tcgacgtatt caatgacgag ctgcacacaa
     3661 atttgccgca cgacgaatac gacacgttgg gcggctggct ggcggccgaa ctggggctca
     3721 tgccgcgccg gggcgacagc atcgtttgcc agggattgaa cattactgtc gtcggagctg
     3781 acgccagacg tgcgctgtgg cttcacgtac aacccgagga acgcgattca tcttcgcagt
     3841 ccaacgccgc cgggtagatt tttgaatcgc ccaaaaagtt cctgcaagcg tgcggctggg
     3901 ctggtgctgg ccggtgcgat ccacgccctg tcctttgcag tcggcccatt tccaggctgg
//
LOCUS       DATCXU010000406         7829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_622743,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000406 DATCXU010000000
VERSION     DATCXU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622743"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VIR04_09150"
     CDS             <1..396
                     /locus_tag="VIR04_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEY9460955.1"
                     /translation="QPYRLEMGTRLATHKGHTLYSYWGPTIAHYLNERLQKQRRPLVL
                     NLASEEYFKSVDLKTLRAPVAHCIFQDFKNGTWKVISFHAKKARGLMARFVIEKRIGS
                     VAGLKAFSAGGYAFDAGQSTDSRLVFKRS"
     gene            complement(399..2651)
                     /locus_tag="VIR04_09155"
     CDS             complement(399..2651)
                     /locus_tag="VIR04_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEY9460956.1"
                     /translation="MASRIPTLQRFFYSHFFFGGVRHTVGIFVPVLVLGGLFGQYRLG
                     MIAAMGALSIAIIDQPGGPRRYRSNEMLAGIALGTLTVAITALASSHSFLIWLTVPIL
                     CFLLSMLTVFGKRGGVIGFACLLLMTLTMRTPLESNEVIASIIYSLMGSLFYFSFSFF
                     LSRALWHRQLQQTLSVALFATADYIMARSRFYDMGTDLDANYRELIRTQSAMIDMHQA
                     TRDMVLRELPKGHGHGDHKRIVILNLFLGMVDLLDSLVATHTDYATLRRELPDSDTLI
                     FARDALYKLATSVSRIALDISRNKQSKERSSVKAELHAIEYELERYKKCGLNQQQPEV
                     YALLVQVLRRLRNATRIVTSMADQTLRSNAHLSADQRLDKSITRFLTRNELRAGMLSS
                     NLRMDSSHFRYAVRVAIAALIGMTVTAIMDQFVANEGMPSSLSTHGYWIILTIVIVMK
                     PGFALTRQRNGWRLTGTLIGCALTFAVFNLTGNSTIYVAVLVAACILGNSLVQLNYML
                     AAMFNTVFVLLGFHFLTPDVQFVISERLADTVIGCSVALLCSYVLPWWEHGFMASLSQ
                     AAKTANQDYLKAGLRYATLCRAQRNAQDLAAADQIKLDAEQAEAEVDWRLARQRVHIA
                     FSNFAAAFYRMMDEPVARQENVPELNHLLIQNHVLASQISAAIPLLAVLPAVPTGIQQ
                     ALDSISDLLDGKEPAPPTSIETDGELATLAYPLRQMIKAAQLIRQEMRGLTAPAPPAD
                     SSQAPVTAAP"
     gene            2898..4115
                     /locus_tag="VIR04_09160"
     CDS             2898..4115
                     /locus_tag="VIR04_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9460957.1"
                     /translation="MRQLRFVLAAGVTALIGASASAIAAEPIVIGVSLTQSPPGSVVQ
                     GTQVKDGVDIVTKMINDAGGVLGRPLKIIYEDNQGIPEKGRAAVEKLITLDKAVAITG
                     GHQSSVCLAEIEVAHRYKVPYVDTNCWSDDIRKKGYPEVFNPANYNTRVASAMAQTIA
                     ALKVKNVVAFAENTDYGINQAKTLAALLKEQSPDIHYKYVTLDRASRDFTPAVLPLRA
                     NPPDLIANMMLPPAAYILMNQLYEQGVAPTAKTWFYDGAGIADYPDFRQNVKEASKYM
                     LVFGLYHPKMVLPKMGQEVADIYKKKFKTAPNRLIFQAADSVVVIAQAIEHAKSTDSA
                     AIIKAMQTMEFDGVRGKFRFSEKPGITYQQWVDIPYVTYQMQEVDQPVDDTVLVQSFG
                     QPLQMDKLVKPAK"
     gene            4182..5072
                     /locus_tag="VIR04_09165"
     CDS             4182..5072
                     /locus_tag="VIR04_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460958.1"
                     /translation="MLALDLLINGLIIGLFYALMAVGLTMIFGILKIVNFAHGEFYMI
                     GAYTYTLISLGLGVSPWLALPIAALAGAGIGWATERLLMRPLYSGYGTWGLMKDEYSI
                     VVTFGLSLLLINLVDKIIGPYPVRGPSLSSVTRVAVGPFMMTGQKLITAGITIVLLVA
                     LALFIKRSLWGRQIQAVAQNRLGASIAGIDIARASSVVFMASGVLAALAGALLSPVIN
                     ATPDVGIFPAVKSFAIVVIGGMGSVPGSILAALLLGVIESFGAVYLSYDYRDTYGLVL
                     LILILLVRPHGLFGERSREV"
     gene            5076..6047
                     /locus_tag="VIR04_09170"
     CDS             5076..6047
                     /locus_tag="VIR04_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9460959.1"
                     /translation="MLILRHPNLHSSKLRNELRLSLAILAVLALMPVTVTSPYWLGVL
                     IVSMYFAIMASGWNLLAGYTGQFSLAPAAFAMLGGYTTGLFAYHLNSPLWIGLPMSFI
                     VPGLIGLALGRIVLRLSGHYLALTTLCFAEIARLVIYNSFDFTRGDQGLNVPGLFDNR
                     LAYYYLFLATLIAVVLLIYWVLRSPAGRFLQAIRDDEIGAASRGVDVVRYKTMAFFIS
                     CAICGFAGGLYGTFARLVSPELGLIAQTGLVVAMVVIGGLGTLVGPLIGAMLVYTASE
                     LLRSVGGIQMIVFAFLVIVFARFFREGLWGLARRAAARRAPTEAGEA"
     gene            6047..6784
                     /locus_tag="VIR04_09175"
     CDS             6047..6784
                     /locus_tag="VIR04_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460960.1"
                     /translation="MALLEIKGIEKRFGGLRAVDGVGIDVHEGELLGLIGPNGSGKTT
                     LLNVLSGHLRADGGSVRLDGKNILGLNPTQLARLGVLRMFQITRLFNRMSAFDNLAVC
                     GMAMGLTQDQSFARAIELLDELKLTPVMYLDAGQLSGGQRKLLEFGTCFMAPPRIALL
                     DESFAAVHPTMKEIMSRFIRRRNDAGQTFVLVSHDMPLVVELCKRSVCMNAGKVLADG
                     PTQQVLNSRPVIAAYLGEESDEELQHA"
     gene            6777..7511
                     /locus_tag="VIR04_09180"
     CDS             6777..7511
                     /locus_tag="VIR04_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460961.1"
                     /translation="MPETLLEMNGVTAGYMGDIDVLRGVSLTVLQGRISGLIGLNGAG
                     KSTLFKTIYGFLQPKTGVVRLAGNDISGKAPHTLIDDGLWYIPQESSLFPYLTVEENL
                     RLPLEGRRKQYGSVLEQRLDDTLTRFPLMREKFKAQAGDLSGGQQKSLEFAKAYMVQP
                     RVCLIDEPSIGLAPKVAVEVFQWIKAFAESGMGILLVDHNVRRVVSMSDHIYVLSLGE
                     ITASGTPEDFRGDLHAQVREWLGINF"
     gene            complement(7501..>7829)
                     /locus_tag="VIR04_09185"
     CDS             complement(7501..>7829)
                     /locus_tag="VIR04_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY9460962.1"
                     /translation="ATAADLVTMAEGDHAFHACIFEIAASPLMLDIVENINDVVERSR
                     QLAFADPARVSEPIAEHTRIIDALAGGSPERAVQAMREHLRNVADRTGVHLEISGAMA
                     MVPVRN"
BASE COUNT         1649 a         2043 c         2310 g         1827 t
ORIGIN      
        1 cagccgtatc ggctagagat gggaacacgg ctggcgacgc acaaaggtca tacgctctac
       61 agttattggg gccccacaat agcccattat ttgaatgagc ggctacagaa acagcgccgg
      121 ccgttggtgt tgaatctggc gtctgaagaa tatttcaagt cggtcgattt aaagacgctt
      181 agggctcccg tggcgcactg catctttcag gatttcaaga acggtacatg gaaagtaatt
      241 agctttcacg ccaaaaaggc gcggggcttg atggcgcgtt tcgtaattga aaaaagaatc
      301 ggcagcgttg cggggctaaa ggcattctcc gccggggggt acgcgtttga cgcgggccag
      361 tcgactgaca gcaggctggt atttaagcgc agttaatttc aaggcgcggc tgtgacgggc
      421 gcttgactcg aatcggccgg cggcgctggc gcagtcaaac cgcgcatttc ttgccgaatc
      481 agttgtgcgg ccttgatcat ttgccgcaaa gggtacgcca gggtcgccag ttcgccgtcg
      541 gtttcaatcg acgtaggcgg cgcaggctct ttaccgtcga gcaggtcgct aatggaatca
      601 agcgcttgtt gtattccggt gggtacggcg ggcagtaccg ctaaaagcgg gattgccgcg
      661 ctgatttgcg atgccagcac atgattctgt attagcagat gattcagctc gggcacgttt
      721 tcctggcgcg cgacgggttc gtccatcatg cggtaaaagg cggctgcgaa attgctgaag
      781 gcgatatgga ccctttgacg cgcaagacgc cagtcgacct cggcttcggc ctgctcggcg
      841 tcaagcttta tctggtcggc agccgccaag tcttgtgcgt ttcgttgagc gcggcatagc
      901 gttgcgtagc gcagccccgc tttcaggtaa tcctgattgg ctgttttggc cgcttgcgac
      961 agcgacgcca tgaaaccgtg ttcccaccac ggcagaacgt agctgcacag cagggccacg
     1021 ctgcatccaa tgaccgtatc cgccagccgc tcgctaatga cgaattggac gtcgggtgtc
     1081 agaaaatgga atcccaacag cacaaaaaca gtattgaaca tggcggccag catgtagttc
     1141 agttgcacca gactgttccc cagtatgcac gccgccacca ggactgcgac atagatagtg
     1201 ctgttgccgg tcaggttgaa tacggcgaaa gtcagcgcgc acccgatcag ggttccggtg
     1261 agacgccagc cgttacgttg ccgggtcagt gcaaagccgg gcttcataac gatgacaatc
     1321 gtcagaatta tccagtagcc gtgcgtgctc agggaagacg gcatgccttc gtttgccacg
     1381 aactgatcca tgatggccgt aacggtcatg cctatcaatg cggcgatggc cacgcgcaca
     1441 gcgtagcgaa agtgcgaaga atccatccgc aaattgctgc tgagcattcc cgcgcgcagt
     1501 tcgttgcgcg tcaggaagcg ggttatggat ttatccagcc gctggtcggc ggacaggtgc
     1561 gcgtttgacc gcagtgtttg gtcggccatg ctggtgacga tgcgtgtcgc attgcgaagg
     1621 cgccgcaaca cctgaaccag caaggcataa acttcgggct gctgttgatt taacccgcat
     1681 tttttatagc gctccagttc gtattcgatg gcgtgcagtt cggccttcac gctgctgcgt
     1741 tctttacttt gtttgttgcg tgaaatatcc agggcaatgc ggctgacgct ggtcgcgagc
     1801 ttgtaaagcg catcgcgcgc gaagataagc gtgtcgctgt cgggcagttc gcgccgcaag
     1861 gtggcgtagt cggtgtgagt ggcgacgagg ctatcgagca gatcaaccat tcccagaaat
     1921 aaattgagta tgacaattct cttgtggtcg ccgtgtccat gtccttttgg cagttcacgc
     1981 agcaccatgt cgcgggtggc ctggtgcatg tcgatcatgg ccgattgggt ccggattaat
     2041 tcccgataat tggcatccag atcggttccc atatcgtaaa accgggaccg cgccataatg
     2101 taatcggcgg tggcaaataa ggcgactgat agtgtttgct gcaattgccg atgccaaagg
     2161 gcacggctaa gaaaaaagct gaatgaaaaa taaaacaagc tgcccattaa tgagtagatg
     2221 atggatgcaa tgacttcatt ggactccagc ggcgtacgca tggttaatgt catcagcagc
     2281 aagcaggcga aaccaatgac gccgccgcgt ttgccgaaca cggtcagcat ggataaaaga
     2341 aaacaaagaa tcggcactgt cagccagatc aggaacgaat gcgacgaggc aagcgccgta
     2401 atggcgactg tgagcgtacc cagcgcgatg ccagccagca tttcgttgct gcggtagcgc
     2461 cttggacctc ccggctggtc gatgatcgca atcgacaggg cgcccatggc ggctatcatg
     2521 cccaggcggt actggccgaa caggccgccc agtacaagaa caggaacgaa tatgccgacg
     2581 gtgtggcgta cgccgccgaa gaaaaaatgg ctgtaaaaaa aacgctgcag cgtcgggata
     2641 cgcgatgcca ttcatccgcc gtgttcgtga gattgtctta agtatagagg cggaatgttt
     2701 cccgataaac catcccgcag tgatacgcat gcggagcatt gacaggcgaa ttaaggctgt
     2761 gtatcatctt ttgactggtc tgaccagatg gcgctttgcg ggtacgcgag cagcagattc
     2821 gcgacgggta agactcatga ttaaaaaaag ggtatgaacc cggcaaggcg ttaccacagg
     2881 aataaaggag acattcaatg agacaacttc gcttcgttct tgccgccgga gtcacggcgt
     2941 taatcggcgc ctcggcatcg gcaatcgccg ccgagccgat cgttatcggc gtcagcctta
     3001 cccagtcgcc acccgggtct gtggttcagg gcacccaggt taaagatggc gttgacatcg
     3061 tcaccaagat gattaatgat gccggggggg tgctcggacg gcccttgaaa ataatttatg
     3121 aagacaacca gggcatacct gaaaaaggtc gcgcggcagt cgaaaaattg attacgctgg
     3181 ataaggcggt tgccattacc ggcgggcatc aaagctcggt atgcctggcc gaaatcgaag
     3241 tcgcacatcg ctataaggtg ccttatgtcg acaccaattg ctggtccgac gatatacgca
     3301 aaaaaggcta cccggaagta ttcaaccccg ccaactacaa cacgcgggtt gcctcggcca
     3361 tggcgcagac catcgccgcg ttaaaggtga agaacgtcgt tgcttttgcc gaaaataccg
     3421 attacgggat caatcaggcc aagacgcttg ccgcgcttct caaggaacaa tcgcccgaca
     3481 tacactacaa gtacgtcaca ctggatcgcg ccagcaggga cttcactccg gccgtgctgc
     3541 ctctgcgcgc caacccgcct gacctgatcg ccaatatgat gttgcctcca gcggcatata
     3601 tattgatgaa tcaactttac gaacagggcg tggcgcctac cgctaaaacc tggttttacg
     3661 atggcgccgg cattgccgac tatccggact ttcgccagaa cgtgaaggaa gcaagcaagt
     3721 acatgctggt cttcggtctg tatcacccca aaatggtcct gccaaaaatg ggacaggaag
     3781 tcgccgacat ttacaagaag aagttcaaga ctgcgccaaa tcgcctgatt ttccaggctg
     3841 ccgattcggt cgtggtgatt gctcaggcaa tcgagcacgc caaatccacc gacagcgccg
     3901 ctattatcaa ggcgatgcaa accatggaat tcgacggcgt gcggggcaag ttccgtttct
     3961 cggaaaaacc cgggattacc tatcagcaat gggtagacat cccgtatgtg acctatcaaa
     4021 tgcaggaagt cgatcagcct gttgacgaca ccgtgctggt tcaatccttt ggtcagcctc
     4081 tgcagatgga caaactggtc aagcccgcca agtaaggggc ttggcgtttg tcgtaacgaa
     4141 caagccgggg cggcgcgccc cggcttccag gagactttat cttgctggct ctcgatctgc
     4201 ttatcaatgg cctgattatt ggtttgtttt atgccctgat ggccgtcggc ctgacgatga
     4261 ttttcggcat cctgaagatt gtgaactttg ctcatggcga gttctacatg ataggcgcct
     4321 atacctatac tctgatttca cttggtctgg gcgtatcgcc atggcttgct ttaccgatag
     4381 cagcgctggc tggcgcggga atcggatggg cgaccgagcg cttgcttatg cgtcccttgt
     4441 actcgggtta cggaacctgg ggactcatga aggacgaata ttcgattgtc gtcacctttg
     4501 gcctgtcctt gctgttgatc aatcttgttg acaagatcat cgggccttac ccggtgcgcg
     4561 gcccgtcgct ttcaagcgta acgcgtgtcg ccgtcggccc gttcatgatg accggccaga
     4621 agttgatcac cgccgggatc accattgttc ttctggtcgc gttggcattg ttcatcaaac
     4681 gctcattatg gggccgccag attcaggcgg ttgcccagaa tcgtctcggc gcctcgattg
     4741 ccggcattga tatcgctcgg gccagcagtg ttgtctttat ggcgtcgggc gtgctggccg
     4801 ctctggcggg ggcgctcctt tcaccggtta tcaatgcaac acccgacgtg ggcatttttc
     4861 ccgctgtcaa atcctttgcc attgtggtga tcggcggcat gggatcggtg ccgggcagca
     4921 ttctggcggc gctgttgctg ggcgtgattg aaagctttgg cgcggtctat ctgtcgtatg
     4981 actatcgcga tacctatggt ctggtgctgt tgatcctgat cttgctggtt cgccctcatg
     5041 gcttgtttgg cgaacgctcg cgcgaggttt aacccatgtt gatattgcgg catccgaatt
     5101 tacattccag caagttgcgc aacgagttgc gtcttagcct ggccatactt gcagtgctgg
     5161 cgctaatgcc ggtgacggtt acttcgccct actggctggg cgtattgatc gtcagcatgt
     5221 atttcgcgat catggcgtcg gggtggaatt tgctggcggg ttacacgggg caattttccc
     5281 tggcaccggc tgcctttgcc atgctgggcg gctataccac ggggcttttt gcttatcatc
     5341 tgaattcacc cttgtggatc ggattgccga tgtcgtttat tgttcccggt cttattggtc
     5401 tggcgctagg gcgaattgtg cttcgcttgt cggggcatta tctggcgctg acaaccttgt
     5461 gctttgccga gatcgcgcgc ctggtcattt ataactcctt tgatttcaca cgtggcgacc
     5521 agggcttgaa cgttccggga ctgttcgaca atcgcctggc ttattactac ctcttcctgg
     5581 ctacattgat tgcggtcgtg ctgctcattt attgggttct gcgttctccg gccggacgat
     5641 tcctgcaagc cattcgcgac gatgaaatcg gggcggcaag ccggggtgtc gacgtggtgc
     5701 gatataaaac aatggcgttt ttcatcagtt gcgcgatctg cgggtttgcc gggggcctgt
     5761 acggcacatt cgcgcgcctg gtcagccccg aactcggctt gatcgcgcag accgggctag
     5821 tcgtggcaat ggttgtgatt ggcggcctgg gaactctggt gggtccgctg atcggagcca
     5881 tgctcgttta caccgcatcc gagttgttgc gcagtgtcgg cggcatacaa atgatcgtgt
     5941 ttgccttcct ggttattgtg tttgcgcgtt tcttccgtga aggcttgtgg ggtctggcgc
     6001 ggcgcgccgc cgctcgccgt gcgcccaccg aagccgggga ggcgtaatgg cgctattgga
     6061 aatcaaaggt attgaaaagc gcttcggcgg cttgcgcgcg gtcgatggtg ttggcatcga
     6121 tgttcacgaa ggagaattgc tggggctgat cggcccgaac ggttcgggca agacgacctt
     6181 gcttaatgtg ctgtcagggc atttgcgcgc cgatggcgga tcggtgcggc tggatgggaa
     6241 aaatatattg ggcttgaacc cgacgcaact ggcaagattg ggcgtattgc gcatgtttca
     6301 aatcacacgc ttgtttaatc gcatgagcgc gtttgacaat ctggcggtat gcggcatggc
     6361 catgggcctg actcaggatc agtcctttgc gcgcgccatt gagctgctcg acgagctgaa
     6421 gctgacgccg gtcatgtatc tggatgcggg ccagctttct ggcggtcaaa gaaagctgct
     6481 cgagttcggc acatgcttca tggcgccgcc gcgtatcgca ttgctcgatg aatcgtttgc
     6541 cgccgtgcat cccaccatga aagaaattat gtcccggttt attcgcaggc gtaacgacgc
     6601 gggccaaacc tttgttttgg tcagtcacga tatgccgctg gtggtcgaac tgtgtaaacg
     6661 gtcggtttgc atgaatgcgg gcaaggtatt ggccgacgga cctactcaac aggtattgaa
     6721 ttccaggcct gtcatcgccg cctatcttgg cgaagaatca gacgaggaac tgcagcatgc
     6781 ctgagacgtt attggaaatg aatggcgtga ccgccggtta catgggtgat atcgacgtgt
     6841 tgcgcggagt ttcgctcacg gttttgcaag ggcgcatcag cggcctgata gggttgaatg
     6901 gcgccggcaa atcaacgctg ttcaaaacca tctatgggtt tttacagccc aagaccggcg
     6961 tagttcgtct tgccggaaac gacatttcgg gcaaggcccc tcatacgttg atcgacgatg
     7021 gcctttggta tataccgcaa gaatccagcc tgtttccata cctgacggta gaggaaaacc
     7081 tgcgtttgcc gctggaaggc cggcgcaagc agtacggcag cgtgctggag cagcgcctgg
     7141 acgatacgct gacccgattt ccattgatgc gtgaaaaatt caaggcacag gcaggcgact
     7201 tgtcaggtgg tcagcagaaa tcgcttgaat ttgccaaagc ttacatggtc cagccgcgcg
     7261 tatgcctgat cgacgaaccc agcattggcc tggctccgaa agtcgcggtc gaagtttttc
     7321 aatggatcaa agcgtttgcg gaatccggca tgggtatatt gctggttgac cacaatgtgc
     7381 ggcgcgtcgt gtcgatgtcg gaccatatct atgtgcttag ccttggggaa atcaccgcct
     7441 cgggaacgcc agaggatttt cgcggcgatc tgcatgcgca ggtccgcgag tggctgggca
     7501 ttaatttcta acgggtacca ttgccatcgc gccggaaatc tccaggtgca ccccggtgcg
     7561 atccgccaca ttgcgcaaat gttcacgcat ggcttgaacc gcgcgttcgg gtgacccgcc
     7621 cgcgagagcg tcgatgatgc gggtatgttc ggcaataggc tcgctcaccc tggccggatc
     7681 ggcaaaggca agctgacggc tgcgttccac gacatcgttg atattttcga cgatatccag
     7741 catgagtgga ctagctgcaa tttcaaagat gcaggcatga aaagcgtggt cgccctcggc
     7801 catcgtgaca aggtcggccg ctgtggcgg
//
LOCUS       DATCXU010000407         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_671360,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000407 DATCXU010000000
VERSION     DATCXU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671360"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..966)
                     /locus_tag="VIR04_09190"
     CDS             complement(115..966)
                     /locus_tag="VIR04_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9460963.1"
                     /translation="MNKRAKVVFLTISAMTFFAANSLLCRVSLTQTAIDPASFTFVRI
                     VSGALALWLIAQFHGAALRGHGNWVSGGSLFLYCASFSLAYLALTAGTGALILFGMVQ
                     ITMIGHGLWRGERPSVRQVIGLVIAFGGLCWLVLPGLSAPPWRGSLLMMVAGVAWGAY
                     SIRGKGTASPTLATAGNFLRAVPFAAGFGILAFPWLSLDAAGILYGATSGAITSGLGY
                     VVWYTALGGLSSTQAATAQLTVPAIAAGGGTLFLAEPITWHLIISSLAILGGVAVVVF
                     GKERRHG"
     gene            1140..1658
                     /locus_tag="VIR04_09195"
     CDS             1140..1658
                     /locus_tag="VIR04_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol dehydrogenase family protein"
                     /protein_id="HEY9460964.1"
                     /translation="MKNSGDVAETLQAYSPKRRRLLATLLSAYTTSLIPWAAAQAVAD
                     DSHGAFLAISAILAGRQSLDSALAKRLYDALVAYDPGFPMAASALLTLINEQKIDPIQ
                     LQKRLDAEHSALAPLPRMIVTAWFMGIVGEGDRARCIAYENALNAVIVEDVLKPPTYA
                     YGVYGSWSKKPV"
     gene            1669..3300
                     /locus_tag="VIR04_09200"
     CDS             1669..3300
                     /locus_tag="VIR04_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY9460965.1"
                     /translation="MPESLSADFIVIGSGIIGSLTAIKLLRQGAAVIILEAGPRITRG
                     EIVARFRNSTRKGDWMSPYPPVPWAPHPIYQPADNGYLIQAGPYPYPAEYIRQVGGTT
                     WHWAAQAWRVVPSDLRIHSLYGVGVDWPITYEELDPYYQEAEEIMGVSGLPDTGSPRS
                     KPFPMEPVAETWAMRRIRERLAPAGYEVVGNTTARNSRSYGGRPACVGNNSCQPICPI
                     DAQYHGGIAAAGAETAGATLIPNAVAYRIEHDPQGHIAAVHYHNPDKTSHRVTGKTFI
                     LAANGIESPKLLLISASDKYPAGLANSSDMVGRHLMDHPSTSITFDADEELWLGRGPQ
                     SPNSINTMRDGAFRSDYSAYRLDFTNISRVLGATQSLIAEGVYGVELAKRLRHTAAYE
                     MSLKNVLEALPHPDNRITISPEKDAMGIPKPVAHYSIDSYTRKGAEVSKKDFARIAEL
                     MGGVNLRYSQDGVFANNQHITGTLSMGNDPKTSVVDSYGRTHDHENLFIVGTGVMPTA
                     ATCNSTMTGMALALRTAEHLLPASVKNQTSNAHTS"
BASE COUNT          828 a         1063 c          927 g          629 t
ORIGIN      
        1 ggcacttatc gtgctcgccg cagccgcgct gatcggcact tggttatttc cggtggacca
       61 taaattgaaa accgcttgaa agccaaataa tcagtgagcc tgggccgaaa ttttttaccc
      121 atgccggcgc tccttgccaa aaacgaccac ggcaacgccg ccgagtatcg ccaaggaaga
      181 tatgatcaga tgccaggtaa taggttcggc cagaaacagc gtgccgcccc cggccgcaat
      241 ggccggcacg gttagctgag ccgtcgccgc ctgggttgag ctcaatccgc ccagcgcggt
      301 gtaccagacg acgtaaccca aaccggaagt aatcgcgcct gatgtcgcgc catacagaat
      361 cccggccgca tccagcgata gccacgggaa ggcaagaata ccgaaacccg cggcaaaggg
      421 gacggcccgc agaaaattcc cggctgttgc aagcgtcgga ctggccgtac ccttgccgcg
      481 aatcgaataa gctccccaag caacccccgc gaccatcatc agcagcgaac cccgccacgg
      541 aggcgcggac aaccctggca gaaccagcca gcacagcccg ccaaaggcaa tcacaaggcc
      601 aatgacctga cgcaccgacg ggcgctctcc gcgccacaag ccatggccga tcatggtgat
      661 ctgcaccata ccaaacagga tcaaggcgcc cgtcccggcc gtaagcgcca aataggcaag
      721 tgaaaaggag gcgcaataaa ggaataatga gccgcctgag acccagtttc catgaccgcg
      781 caatgccgcc ccatgaaact gagcgatcag ccacaacgcc agggctccgg acacgatccg
      841 aacgaatgta aagctggccg gatctatcgc cgtctgtgtg agcgatacgc ggcaaagcaa
      901 tgagttcgcc gcaaaaaacg tcatggcgga gatcgtcaga aaaaccactt tggcgcgttt
      961 attcatggcg aaatctccgc aaactcttat tttatgccac cacttcactc cccggcacag
     1021 ccgtccttgc caggcgtggc gctttaaata agcactcgat aaaacacgtg cataaagcat
     1081 ttaaaaaagt gtaagattgt caaggcaagc tatcccctgt ctaccaatcg ggcgctgata
     1141 tgaagaattc aggcgacgtg gcggagacac ttcaggctta tagcccgaag cgcagacggc
     1201 tgctggccac cctgttgtcg gcctacacca catccctgat tccatgggcc gccgcgcaag
     1261 ctgtcgcgga cgacagccac ggagcctttt tggcgatatc cgcgatactg gccggccggc
     1321 aatcgctgga tagcgcactt gccaagcgac tgtatgacgc gctggtcgcc tacgacccgg
     1381 gctttcccat ggcggcaagt gccctgctca cgctaattaa cgaacagaaa atcgatccga
     1441 ttcaactgca aaagcggctt gacgctgaac attccgcgct ggcgcctctg ccccgcatga
     1501 ttgttaccgc ctggttcatg ggtatcgtgg gcgagggcga ccgcgcgcgc tgcattgcct
     1561 acgaaaacgc cttgaacgcc gtaatcgtcg aagatgtcct gaagccgcca acttacgctt
     1621 atggcgtcta tggaagctgg tcgaaaaagc ccgtctgagg agcaccagat gccggaatca
     1681 ttgtccgccg actttatcgt aataggttca gggattattg gctcgctgac tgccatcaaa
     1741 cttttgagac aaggcgctgc ggtcatcatt ctggaggcgg gaccacgcat tacacgcggc
     1801 gagattgttg cgcgctttcg aaactcgacg cgcaagggcg actggatgtc cccctatccg
     1861 cccgtgccat gggcgccgca cccaatttac caacccgcgg acaacggcta tttgattcaa
     1921 gcaggcccat atccctatcc cgccgaatat attcgccagg ttgggggtac gacgtggcac
     1981 tgggctgcgc aagcctggcg cgtcgtcccc agcgacctgc gtattcacag cctgtacgga
     2041 gtgggtgtcg actggcccat tacctatgaa gagctcgacc cctattatca ggaagcggaa
     2101 gaaataatgg gagtctccgg gctgcccgat acgggatcgc cgcgcagcaa gccatttccg
     2161 atggagcccg ttgccgaaac atgggccatg cgccgtattc gcgaacgcct ggcgcctgcc
     2221 ggctatgaag tggtcggcaa caccactgcg cgcaacagca gaagctatgg gggtcggcca
     2281 gcatgtgtcg gcaacaacag ttgtcagccc atctgtccga tcgatgctca gtatcacggc
     2341 ggtattgcgg cggccggcgc cgaaacggcg ggcgcaactc tgattccgaa cgccgtcgcg
     2401 taccgaatcg agcacgatcc ccagggccac attgccgcgg ttcactacca caacccggat
     2461 aagacctctc accgtgtcac cggcaagacg ttcattctgg cggcgaacgg gattgaaagc
     2521 cccaaactgt tattgatatc ggccagcgac aagtaccccg cagggctggc caacagttca
     2581 gacatggtcg gccgccatct gatggatcac cccagcacat cgatcacatt cgacgccgat
     2641 gaggaattgt ggcttgggcg cggcccgcaa agcccaaatt cgatcaacac gatgcgcgat
     2701 ggcgctttcc gctcagacta ttcggcgtat cgccttgatt tcaccaatat ctcgcgagta
     2761 ctgggcgcca ctcagtcact gatcgccgaa ggcgtatacg gcgttgaact ggcaaaacgc
     2821 ctgcgccaca ccgctgctta cgaaatgagt ctgaagaacg tactggaagc cctgcctcat
     2881 ccggacaatc gcatcaccat aagccccgaa aaagatgcga tgggcattcc aaaacccgta
     2941 gcccactatt caattgactc gtacacccgc aaaggagccg aggtctcaaa gaaggacttt
     3001 gcgcgtatcg ccgaactgat gggcggcgtc aacctgcgtt atagccagga tggtgttttt
     3061 gccaacaacc agcacatcac cggcactctg agtatgggca acgacccgaa gacgtcggtt
     3121 gtcgacagct acgggcgtac gcacgatcac gagaatctgt ttattgttgg caccggcgtt
     3181 atgccgaccg cggcgacctg caactcgacc atgaccggaa tggcccttgc gctacgcacc
     3241 gccgaacacc tgttgccggc atccgtaaaa aaccagacat cgaacgcgca cacgtcgtaa
     3301 tagttacttt taaggcaggg tttaaaaagc ttatgacacg cctgcactat gtcctgaata
     3361 ttgcgctgct ggtggcgcca ggctggaata catatgccgc caccaccaca atccccgtcg
     3421 acacggcaag cacgcaattg attcagc
//
LOCUS       DATCXU010000408         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_829048,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000408 DATCXU010000000
VERSION     DATCXU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829048"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VIR04_09205"
     CDS             <1..321
                     /locus_tag="VIR04_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY9460966.1"
                     /translation="QHPDSLMELQKVQARILRQASTCVAPGGRLVYATCSLLPEENER
                     QIESFLAENDGFTLLDASKIVRDRCENLTLAGPYLSLRPDRHGTDGFFAAVLERAKPS
                     PRRN"
     gene            394..1611
                     /locus_tag="VIR04_09210"
     CDS             394..1611
                     /locus_tag="VIR04_09210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY9460967.1"
                     /translation="MDNVLAFLSGGLLQLTWWQVVLVTLAITHVTIVSVTVFLHRSQA
                     HRGLDLHPAAMHFFRFWLWLTTGMVTKEWVAIHRKHHAKCEREGDPHSPAVFGLGKVF
                     FEGAELYRLEAHNTDTLTRYGRGTPDDWLETHLYTGHSMLGIVLMLIIDLSLFGVLGL
                     TVWAVQMAWIPFWAAGVVNGLGHFFGYRNYACPDQSTNVSPWGIIIGGEELHNNHHAY
                     GTSAKFSTKWYELDIGWGYIQILRFFRLATVKKVAPKLKLEPVSAKMSIDLSTLQGII
                     THRYEVLARYTDLVKKSARDELDRLKLTRKEGDADCGWALLRRAKKWLYRADDDALKP
                     EQRAQLDTVLARNQALSTLVQMRRELVRLWESSSESSEQLLGDLQAWCQRAQQSGIEG
                     LEQFSYRLRRYAA"
     gene            1608..>3589
                     /locus_tag="VIR04_09215"
     CDS             1608..>3589
                     /locus_tag="VIR04_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY9460968.1"
                     /translation="MIDLQLNPQQQAAVTLPAQHALVLAGAGSGKTRVLTTRMAWLIQ
                     TGQASPFELLAVTFTNKSAREMLARLTTLLPINTRGLWVGTFHGLCNRLLRAHYRDAG
                     LSQTFQILDSADQLAAIKRLIKVLGIDDDKYAPRDVQHFINGAKEQGQRPADIHARDA
                     HQDRLIEIYRLYQAQCERESVVDFAELLLRAYELLQRNAPIREHYQRRFRHILVDEFQ
                     DTNTLQYRWLALLAGGGAAIFAVGDDDQSIYAFRGANVGNMADFRRDYAKDSVIRLEQ
                     NYRSYGHILDSANALIAHNSSRLGKNLWTEQGVGEPVRVIELPSDLLEARWLVEEIKA
                     LINDGRLRRECAVLYRSNAQSRVIEHALFAAGISYKVYGGLRFFERQEIKHALAYLRL
                     LDNPNDDTSWLRVVNFPARGIGARTLEQLGDLARQQNSSLYGAVTLLPGKGGGKLLKF
                     VELIQQMAHEAQIVTLPELVEHVIHHCGLRAHYQNEKEGADRLENLQELVNAAAVFVT
                     EENFAGLPAGRAVELPASGAGDLLEQNGAEQTLSPLAAFLTYASLEAGDNQAQAGQDA
                     VQLMSVHAAKGLEFDAVFITGVEDGLFPHENSLLESAGLEEERRLMYVAITRARERLY
                     LSLAQSRMLHGQTRYAMRSRFLAEIPDEHLKWLSPK"
BASE COUNT          760 a          990 c         1023 g          816 t
ORIGIN      
        1 cagcatccag actctttgat ggaactgcaa aaagttcagg cgcggattct taggcaagca
       61 tcgacatgcg tggctcccgg gggccgtctg gtgtatgcca cctgcagtct gctgcccgag
      121 gagaacgagc ggcagattga aagcttcttg gccgaaaatg acggctttac gctattggat
      181 gccagcaaaa tcgtgcgaga tcgttgcgaa aacctgacac tggccggccc gtatctgtct
      241 ttgcggcctg atcgccatgg gaccgacggc ttttttgccg ccgtgctgga gcgcgccaag
      301 ccttcaccgc gacgaaattg aaatactgct gtcatatagc tgacaagtcg gataaacttt
      361 actgttattt acttacaggc cttaatcagg gttatggaca acgtacttgc ttttctctcg
      421 ggtggtttgc tgcaattgac ttggtggcag gttgttcttg tcacgttggc aataacgcac
      481 gtcactattg tttcggttac ggtgtttttg caccgtagcc aggcgcaccg aggtctagac
      541 cttcatccgg ctgccatgca ttttttccgt ttctggcttt ggcttaccac gggcatggtc
      601 actaaagagt gggtggccat ccatcgcaag caccacgcca agtgtgaacg cgagggggat
      661 ccccattcac cggccgtatt cggcttgggc aaagtgttct tcgaaggcgc cgaactgtat
      721 cggctcgaag cgcataacac cgacacatta acgcgttacg gcaggggcac gcctgacgac
      781 tggcttgaaa cccatctata cacgggccac agcatgctgg gcattgtcct catgctgatc
      841 atcgatctgt ctttgtttgg cgttctgggc cttacggtgt gggccgtgca gatggcgtgg
      901 attccgttct gggcagccgg cgtggtcaat ggactgggcc actttttcgg ttatcgcaat
      961 tacgcttgcc ccgatcaaag cacaaacgtt tcaccctggg gcattattat cggtggcgaa
     1021 gaactccaca ataatcacca tgcctatggc acatcggcca aattttcgac caaatggtac
     1081 gaactggata tcggctgggg ttatatccag attcttcgct ttttccgcct tgccaccgtt
     1141 aaaaaagtgg cgccgaagct taagcttgaa cctgtctcgg ccaagatgtc gatcgattta
     1201 agcacattgc agggcattat cactcaccgc tacgaggtcc tggcgcgcta taccgatctg
     1261 gtgaaaaagt cggctcgcga cgagctcgac agactcaaac tcacgcgcaa ggaaggcgat
     1321 gccgattgcg gctgggcctt gctcaggcgc gccaagaaat ggctttatcg cgccgacgac
     1381 gatgcgctca aacccgagca gcgtgcgcaa cttgacaccg ttctggccag aaaccaggct
     1441 ttgtcgacgc tggtccaaat gcgccgcgaa ctagtccgcc tttgggaaag ttccagtgaa
     1501 agcagcgagc agttgctggg cgatttgcaa gcctggtgcc agcgcgcgca acaaagcggc
     1561 atcgaaggac tcgaacagtt ctcttatcgg ttgcgccgct acgcggcatg atcgatctgc
     1621 agcttaatcc gcaacagcaa gctgccgtta ctcttcccgc tcagcacgca ctggtgcttg
     1681 cgggcgcggg aagcggaaaa acccgggttc tcaccacgcg gatggcgtgg ctgattcaaa
     1741 ccggccaggc cagtcctttc gagctcctgg cggttacgtt taccaacaag tcagcgcgcg
     1801 aaatgctggc gcgtttgacc accttgctgc ccatcaatac gcgcggcctg tgggtcggca
     1861 cttttcatgg tctgtgcaat cgtttgttgc gcgcccacta tcgcgacgca ggcctgtcac
     1921 agacctttca aattctggat agcgccgatc aactggcggc catcaagcgg ctgataaagg
     1981 ttctgggcat tgatgacgac aagtatgcgc cgcgcgacgt acagcatttc atcaacggcg
     2041 caaaagaaca gggccagcgt cccgctgata tacacgcgcg cgacgcgcat caagaccgtc
     2101 tgatcgagat ttatcggttg taccaggcgc aatgcgagcg cgaaagcgtc gtcgacttcg
     2161 ccgaactatt attacgcgcc tacgaactgt tacaacgcaa tgctccaata cgcgagcatt
     2221 atcagcgccg ctttcgccac attctggtgg atgaatttca agacaccaac accttgcagt
     2281 atcgctggct ggctctgctg gcgggtggcg gcgcggccat tttcgcggtg ggcgacgatg
     2341 atcaatcgat ctatgcgttt cgcggcgcca acgtgggcaa tatggcggat tttcgccgcg
     2401 actatgccaa agactcggtg attcggctgg agcagaacta ccgctcctat ggccatattc
     2461 ttgattcggc caatgcgctg attgcgcata attcctcgcg cctggggaag aatttatgga
     2521 ctgaacaggg cgtcggcgaa cctgttcgcg tgattgagct gccctccgac ttgctggaag
     2581 cgcgctggct ggtcgaagag ataaaagctt tgatcaatga tggccggctg cgccgtgaat
     2641 gcgctgttct gtatcgcagc aatgcacaat cgcgcgttat cgagcacgcc ctgtttgccg
     2701 cgggtatttc ctacaaggtt tatggcggct tgcgtttttt tgaacggcag gaaatcaagc
     2761 acgctctggc ctatttgcgt ttgctggaca acccgaacga cgatacatcc tggttgcggg
     2821 tggttaattt tccggctcgc ggcattgggg cccgcacact cgaacaactg ggcgacctgg
     2881 cgcgccagca aaacagcagc ttgtacggcg ccgtgacgct tcttccgggc aagggtggcg
     2941 gcaagttgct caagttcgtc gaacttattc agcaaatggc gcacgaggcg caaatagtga
     3001 cactgcccga gcttgtcgag catgtcattc accattgcgg tttgcgggcg cactatcaaa
     3061 acgaaaaaga aggcgccgac cggctcgaga acctgcagga attagtgaac gcagcggctg
     3121 tttttgtgac ggaagagaac ttcgcagggt tgccagccgg tcgcgcggtt gaattgcccg
     3181 cctcaggcgc tggcgatctg ctggaacaaa atggtgccga acaaaccttg tcgcccttgg
     3241 cggcctttct aacgtatgcg tcgcttgaag cgggcgacaa ccaggcgcag gctggccagg
     3301 atgccgtcca gcttatgtct gttcatgccg ccaagggtct ggagttcgac gctgtcttta
     3361 tcaccggcgt ggaagacggg ctgtttccgc atgaaaatag tcttcttgag tcggcgggac
     3421 ttgaagagga acgtcgcctg atgtatgtcg cgattacgcg cgcgcgcgaa cgcctgtatc
     3481 taagcctggc gcaaagccgc atgctgcatg ggcaaacgcg ctacgcgatg cgctcacgct
     3541 ttctggccga gatccccgac gaacatttga agtggctgag ccccaaaca
//
LOCUS       DATCXU010000409         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_413996,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000409 DATCXU010000000
VERSION     DATCXU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413996"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1648)
                     /gene="ubiB"
                     /locus_tag="VIR04_09220"
     CDS             complement(92..1648)
                     /gene="ubiB"
                     /locus_tag="VIR04_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY9460969.1"
                     /translation="MFTLLRLIRIIFVGLRYRLDELVLSAIKHPLAHGLLHVARLGRT
                     PKTPRGVRLRLALESLGPIFVKFGQVLSTRRDLIPADIAEELALLQDQVPPFPSDQAA
                     ACVEAALGQSPHKLFRHFDTDPVASASIAQVHFAVLHDGREVAVKILRPKMQEVIEKD
                     LRLMRIAAGLVERLVSDGRRLKPLEVVAEFDKYLHDELDLIREAANCSQLRRNFGPGT
                     GRESLLMVPEVVWEYCATTVFTMERMRGIPVSQTQRIEAAGINIQNLARTGVEIFFMQ
                     VFTDGFFHADMHPGNIFVSDDPATLGRYIALDFGIVGSLSEFDKNYLAQNFLAFFRRD
                     YRRVAQLHIESGWVPPHTREEELEGAVRAVCEPYFDRPISEISLGQVLLRLFQTSRRF
                     NVEIQPQLVLLQKTLLNVEGMGRELDPRLDLWDTAKPYLEKWMHQRVGFAGLRKRLEQ
                     EIPQWAQILPQIPRLIHTNLMRPDTSPQLSSEIQRMRRAQEQTNRLLMAIAAVLAVAM
                     GVAIWAVTRY"
     gene            complement(1651..>2114)
                     /locus_tag="VIR04_09225"
     CDS             complement(1651..>2114)
                     /locus_tag="VIR04_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460970.1"
                     /translation="ACDAAILPDVILTIPADKLGRLPAALGKKDPDLIAALMHVQGDA
                     ALASVVSELARELRWDIEDDLAGLVGDVAALRLIKGVKTVFDRTRTSTGRFAGNVGEY
                     LSEEGKLVLGRDAYESWRDRLGGMRERLDSLEQRVQRLDANRPSSRTEGEG"
BASE COUNT          502 a          648 c          554 g          410 t
ORIGIN      
        1 tctgggcaaa gccccggcga gcgccgccgc aatgcatgcc tggcgtgtga gtatcttgga
       61 ttcgaaacgt gcggccatgg tgggcaccct ttcagtaacg cgtaactgcc cagatcgcca
      121 cacccatggc cacagccaaa acagcggcga tcgccatcag cagtcgattt gtttgctcct
      181 gcgcacggcg catacgctga atttcggagc tcaactgagg gcttgtatcg gggcgcatca
      241 agttggtgtg aatcaggcgc ggtatctgag gcaggatttg cgcccactgc ggaatttctt
      301 gctccagacg tttacgcaat cccgcaaaac ccacgcgctg atgcatccac ttttcaaggt
      361 agggcttggc ggtatcccac aaatccaggc gcggatccag ttcgcgcccc atcccctcga
      421 cattcagcaa ggttttctgc aacaacacca gttgcggctg aatttccaca ttaaagcggc
      481 gcgaggtttg aaacagacgc aataacacct ggcccaggga tatttcggaa atgggccggt
      541 cgaaataagg ctcgcaaaca gcccggacgg cgccttcgag ttcctcttca cgcgtatgcg
      601 gtggcaccca tccggattca atatgaagct gcgcaacacg ccggtaatcg cgccgaaaga
      661 aagccagaaa attttgcgcc agataatttt tatcaaattc ggacaacgac cccacgatgc
      721 cgaaatccag cgcaatgtat cgtcccaacg tggcggggtc gtcagacacg aaaatattgc
      781 ccggatgcat gtcggcgtga aaaaaaccgt cagtgaacac ctgcatgaaa aaaatctcga
      841 cgcctgtgcg ggcaagattc tgaatattga tacccgccgc ctcgatgcgc tgagtctgac
      901 tgacgggaat gccgcgcatg cgttccatgg tgaataccgt ggtggcgcaa tactcccata
      961 ccacttcggg caccatgagc aaactttcac gtcccgtgcc gggcccgaag ttgcggcgca
     1021 attgactgca attggcggcc tcgcgtatca gatccagttc atcatgcaga tacttgtcaa
     1081 attcagcgac cacttcaagg ggcttcaggc gtctgccgtc ggagaccagg cgttcaacaa
     1141 gcccggccgc aatccgcatt agcctgaggt ctttttcaat aacttcctgc atcttgggac
     1201 gcaaaatctt gactgcgacc tcgcgcccat cgtgcaaaac ggcaaaatgc acctgcgcaa
     1261 tcgaagccga ggcaacaggg tccgtgtcaa aatgcctgaa cagtttgtga ggcgattgcc
     1321 ccaatgccgc ctccacgcac gccgccgcct ggtccgacgg gaaaggcggg acctgatctt
     1381 gcaataacgc cagctcctcg gcaatgtcgg ccgggatcaa atcgcgccgc gtcgacagta
     1441 cctgcccgaa tttgacaaaa atggggccta gcgattccag agccagtcgc aaacgtacgc
     1501 cgcgcggggt tttgggagtg cgcccaagcc gggccacatg caacaaaccg tgggccagcg
     1561 gatgttttat cgccgacaaa accagttcgt ccagacggta gcgcaagccc acaaaaataa
     1621 tgcggatcag gcgcagcaat gtaaacatgc tcagccctcg ccttcggtcc gcgacgacgg
     1681 ccgattggca tcaaggcgtt gcacgcgctg ctcaagcgaa tcaaggcgtt cacgcatgcc
     1741 gcccaatcga tcccgccagg actcatacgc atcacggccc aataccagct tgccttcttc
     1801 ggacaagtat tcacccacgt tgccggcaaa gcgcccggtg gaagtcctcg tccggtcaaa
     1861 aactgtttta acccctttta tcagacgcag ggcggcaaca tcgcccacca gacccgccag
     1921 atcatcttcg atgtcccatc gcagttcgcg tgccagttcc gacacaacgc tcgccagcgc
     1981 ggcatccccc tgaacatgca ttagcgcggc aataagatcg gggtctttct tgcccaacgc
     2041 agccggcaga cggccaagtt tatcagccgg aatcgtcaat attacatcgg gcaaaatggc
     2101 tgcgtcgcaa gcct
//
LOCUS       DATCXU010000410         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_829839,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000410 DATCXU010000000
VERSION     DATCXU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829839"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1357)
                     /gene="fabF"
                     /locus_tag="VIR04_09230"
     CDS             complement(128..1357)
                     /gene="fabF"
                     /locus_tag="VIR04_09230"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY9460971.1"
                     /translation="MKRRVVITGLGIVSPVGNDVDKAWDNIVNGRSGVGRITRFDTSL
                     FSARIAGEVKDFDVTQVMSAKEAKQMDTFIHYGVAAGMQAWRDSGLDSTDDGAERMGA
                     IVGSGIGGLQRIEEAQIDYLERGPRRISPFFVPASLINLVSGQLSIKLGLRGPSYAVV
                     SACTTGLHAIGDAARLIEYGDADVMLAGGTEASVTPLGVGGFAAMRALSTRNDDPVTA
                     SRPWDVDRDGFVLGEGAGVLVLEEYEHARKRGARIYGEFAGYGMSSDAHHITTPDSDG
                     PRRGVINALHNSGINPDQVSYVNAHGTSTQVGDKNETEALKLAFGDHAKKLVVNSTKS
                     MTGHLLGAAGGIEAVFTALAVYHQISPPTINIFNQDPECDLDYCANQARDLKIDVALS
                     NSFGFGGTNGSMIVRRV"
     gene            complement(1503..1742)
                     /gene="acpP"
                     /locus_tag="VIR04_09235"
     CDS             complement(1503..1742)
                     /gene="acpP"
                     /locus_tag="VIR04_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY9460972.1"
                     /translation="MESIEQRVKKIVAEQLGVNEAEIKNESSFLDDLGADSLDMVELV
                     MALEDEFETEIPDEEAEKITTVQQAVDYITSHSKQ"
     gene            complement(1988..2731)
                     /gene="fabG"
                     /locus_tag="VIR04_09240"
     CDS             complement(1988..2731)
                     /gene="fabG"
                     /locus_tag="VIR04_09240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY9460973.1"
                     /translation="MQEKQLTGKTALVTGATRGIGKAIALELAARGAHVVGTATSDAG
                     ARAISELLADSAGQGIVLDVTDSASCEALMARLGQESGGPHILVNNAGITRDALAMRM
                     KDEDWDIVLNTNLSAVFRLSRAAIKNMMKARWGRIINITSVVGSCGNPGQVNYAAAKA
                     GLAGMARALARELGSRNVTVNCVAPGFIETDMTRALNETQAAAILSQIPLGRLGSPAD
                     IASAVAFLASPGAAYITGTTLHVNGGMYM"
     gene            complement(2737..3666)
                     /gene="fabD"
                     /locus_tag="VIR04_09245"
     CDS             complement(2737..3666)
                     /gene="fabD"
                     /locus_tag="VIR04_09245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY9460974.1"
                     /translation="MKIAFVFPGQGSQSVGMLNAWAGNPVVADTLAQASQALDQDIAA
                     LISNGPSDRLDLTTNTQPVMLAAAMAMYRAWLHAGGPAPELVAGHSLGEYSALTAAGA
                     LTLEDAVRVVRIRANAMQDAVPVGAGAMAVILGLASDAVKAVCSEAALGDVVEAVNFN
                     APEQVVIAGHKGAVDRACELAKQAGAKRALLLPVSAPFHSSLLSPAAAQLQRALASVN
                     LAAPRIPLINNVDVASPEEPAAIKDALVRQACRPVRWVEVIQAMKARHVTHVVECGPG
                     KVLGGLTKRIDRDLIGLSIADPVSLEMTLKALR"
     gene            complement(3680..4666)
                     /locus_tag="VIR04_09250"
     CDS             complement(3680..4666)
                     /locus_tag="VIR04_09250"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY9460975.1"
                     /translation="MNTTVSYSRVVGTGGCLPERIVTNDELAAELATRNVETSDVWIT
                     ERTGIRQRHIADADMTTSLLATQAARLALENAGIAASEIDLIIVATSTADFVFPSTAC
                     QVQANLGIKTGAAFDVQAVCSGFVYALVTADSFIRTGRSKAALVIGAEVFSRILDWSD
                     RSTCVLFGDGAGAVVLKASEEPGIVGAMLNADGSQMNILCAAGNVANGQVVGDPFLRM
                     DGQAVFKLAITSLEQSAKDVCAQAGVTVADIDWMVPHQANIRILSLLARRLGMPAEKL
                     VVTLDKHANTSAASVPLAFDVACRDGRIKAGDLVLMQGVGGGFTWGSVLLRM"
     gene            complement(4663..>5010)
                     /locus_tag="VIR04_09255"
     CDS             complement(4663..>5010)
                     /locus_tag="VIR04_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY9460976.1"
                     /translation="VATMMREEFKRNALSLISGMLARPVLGRLRDRVDNRRYNGAALL
                     GLRGIVIKSHGSADVYAFGCALERAHGAVCNDLLAGTTSAIRLRQNLQSSAALPAEIS
                     NDEPGSEPLSGVR"
BASE COUNT         1131 a         1557 c         1343 g          979 t
ORIGIN      
        1 ccaggaccca aaaaaacaat agccaacgct agttaaagcc gccagcccca acactgcgct
       61 tagcaagacc acccgaaaag gacgctgagc cggccactgc ccgacgcatg tcatggcgta
      121 ctccgattta cacccgcctt actatcatgg agccgttcgt gccgccaaag ccgaaagaat
      181 tcgacaaagc gacatcaatc ttgagatccc gcgcctggtt ggcgcagtag tcaagatcgc
      241 actcgggatc ctgattgaat atattaatcg ttggggggga tatttgatga tagacagcca
      301 atgccgtaaa aacggcctcg atgccgccgg cggcccccag caaatgaccc gtcatggact
      361 tggtggaatt gaccaccaac tttttggcat ggtcgccaaa agccaattta agcgcctcgg
      421 tttcgttttt atcacccacc tgtgtggatg tgccgtgcgc attgacatag ctgacctgat
      481 cgggattgat gccgctgttg tgcaaggcat tgatcacccc tctgcgcggc ccgtcgctat
      541 cgggagtagt gatgtgatgg gcatcgctgc tcatgccgta gccggcgaat tcgccgtaaa
      601 tacgggcgcc gcgtttgcgc gcatgctcgt attcttcaag aaccaaaaca cccgcgccct
      661 cgcccagaac gaatccatcc cgatcgacgt cccaagggcg cgacgccgtg acggggtcgt
      721 cgttgcgcgt cgacaaggct cgcattgccg caaaaccgcc gacacccaat ggggtcaccg
      781 aggcttccgt cccgccagcc agcattacgt ccgcatcgcc atactcgatc agccgggctg
      841 cgtcgccgat agcgtgcaaa ccggtcgtgc atgccgacac caccgcgtaa ctggggcctc
      901 gaaggcccag tttaattgac aactgtccag aaacgagatt gatcagcgat gccggcacaa
      961 aaaagggcga aattcgacgt gggccacgtt caagataatc gatttgcgcc tcttcgatgc
     1021 gctgcaaacc acctatgcca gatcccacaa tcgcgcccat gcgttcggcg ccgtcatccg
     1081 tgctatccag cccggaatcg cgccaagcct gcatccccgc cgccacaccg taatggatga
     1141 atgtatccat ttgcttggct tctttggcgg acatgacttg cgtcacgtcg aaatctttaa
     1201 cctcgcccgc aatccgggcg ctgaacaaag aggtgtcgaa acgcgtaatg cgcccgacac
     1261 ccgagcgccc attcacgatg ttgtcccatg ccttgtccac atcgttgcca acgggcgaaa
     1321 cgatgccaag gccggtaatg acgacacgtc gtttcacgga tggctcctaa aaaaacggat
     1381 tgaagtaaaa acacccatta ccgcgccaat ccggttcgaa atggcacaac ggcgggcctt
     1441 tctcgaagaa acctcgctga cgcggtccgg gatcaagcca agactacgca ataaatgcaa
     1501 aattactgct tgctgtgcga cgtgatgtag tcgacggcct gttgcaccgt cgtgattttt
     1561 tcggcctctt cgtccggaat ctcggtttca aattcatctt caagcgccat cacgagctcg
     1621 accatatcga gcgaatcggc accgaggtcg tcaaggaagg aggattcgtt tttgatctcg
     1681 gcttcgttga cgccaagttg ttcagcgacg atcttcttga cgcgctgttc gatgctttcc
     1741 atgcagatct ccaagtcggg aaaattcccg cgaattatag ccaaatgata atgtattaaa
     1801 agctggctag gacaaaaaaa acggcattac caaaatacca atgacgcttc gcaccatgcg
     1861 aaccgcctat ccggaacacc tctgaacggg acagatttta ccctgttcca cttattattc
     1921 catttctaaa ttggccgtaa acacccgggc aaggaaggca acgcacacgg ccaattcaga
     1981 agtggaatta catatacatg ccgccattga catgcaaggt cgtgccggtg atataagcgg
     2041 cgccgggcga ggccagaaat gccacagcac tggcgatgtc ggcgggcgag cccagacggc
     2101 ccagtggtat ttgcgacaag atggcggccg cttgcgtttc gttaagcgca cgcgtcatgt
     2161 cggtttcgat aaaacctggc gcaacgcagt tcaccgttac attgcggctg cccagttccc
     2221 gggccagagc gcgcgccatg cccgccaggc ccgccttggc cgccgcgtaa ttgacctgac
     2281 cggggttgcc gcatgatccc acgacagatg tgatgttgat aatccgcccc cagcgcgctt
     2341 tcatcatatt cttgatagcc gcgcgcgaca aacgaaacac tgctgacaaa tttgtgttga
     2401 gcacaatatc ccaatcctcg tccttcatgc gcatcgccag cgcatcgcga gtaattccgg
     2461 cgttattcac aagaatatgc ggcccgccgc tttcttggcc aagccgcgcc atcagggcct
     2521 cgcatgaagc ggaatctgtt acatcgagca caatgccctg tcccgcgcta tcggccaata
     2581 gctcagatat ggcgcgcgca cctgcgtcag aggtcgccgt gcccaccaca tgggccccgc
     2641 gcgccgccaa ttccagggca atggctttgc caatcccgcg ggttgcaccg gtcaccagag
     2701 ccgttttgcc agtaagctgt ttttcttgca taaaagtcac cttaacgctt tcagcgtcat
     2761 ttcgagcgac acaggatcgg caatcgacaa accgatcaga tcgcggtcta tgcgtttcgt
     2821 caggccgcca agcactttac cggggccaca ttcgaccaca tgcgtgacat gccgggcctt
     2881 cattgcctgt atgacttcaa cccagcgcac tggccggcac gcctgacgca ccagggcatc
     2941 cttgatcgcc gcgggttcct ccgggctggc aacatcaaca ttattaatca gtggtatccg
     3001 gggcgccgcc aaattaacgc tcgccagggc acgctgcaat tgcgcggcgg caggcgacaa
     3061 caggctggaa tggaaaggtg cggagacagg caaaagcaag gcgcgcttgg cgccggcctg
     3121 cttggcgagt tcgcaggcgc gatcgaccgc gcctttgtgc ccggcgatga ccacttgctc
     3181 gggagcgttg aaattaaccg cttcaaccac gtcgccgagg gcggcctcgg agcatacggc
     3241 tttgacggca tcgctggcca aaccaaggat caccgccatg gcgccggcgc cgaccggcac
     3301 agcgtcttgc atcgcgttgg cgcggatacg cactacgcgc acggcgtctt caagcgtaag
     3361 cgctccggcc gccgtcaagg ccgaatattc acccaggctg tgccccgcca ccagctcggg
     3421 cgccgggccg ccggcgtgca accaggctct gtacatggcc atcgcggccg ccagcataac
     3481 cggctgcgtg tttgtagtga gatcaagccg atcggagggg ccgttcgaaa tcagcgccgc
     3541 aatgtcctgg tccagcgcct gtgacgcctg cgcgagcgta tcggccacca ctggattgcc
     3601 ggcccatgcg ttaagcattc cgaccgactg cgagccctgc ccgggaaaga caaaagcaat
     3661 tttcatcata cgtaatcgct cacatccgaa ggagcacgga accccaggtg aatccgccgc
     3721 ccacgccttg catcagaacc agatcgcccg ccttgatgcg gccgtcgcga caagccacat
     3781 cgaatgccag cggcacactt gcagcggatg tattggcatg cttgtccaaa gtaaccacca
     3841 gtttttcagc aggcatgccc agccgccgcg ccagcaagct caggatgcga atattcgcct
     3901 ggtgcggcac catccagtca atgtcggcga cagtaacccc ggcttgcgcg cagacatctt
     3961 tggctgactg ctccagcgag gtgatggcca atttgaaaac agcctgaccg tccatgcgca
     4021 agaaaggatc gcccaccacc tggccatttg cgacattgcc ggcagcgcat aagatgttca
     4081 tctggctgcc atcggcattt agcatcgcgc ccacgatccc gggctcctca gaggctttta
     4141 aaacgactgc gccagcgcca tcgccaaaca acacacaggt actgcgatcg ctccagtcga
     4201 ggattcttga aaaaacctcg gctccgatta ctaaagccgc tttggaacgg ccagtgcgaa
     4261 taaacgaatc cgcggtaacc agcgcataca cgaatccact gcatactgcc tgcacgtcaa
     4321 acgccgcccc ggtcttgatg cccaaattgg cttgcacctg acacgccgta ctgggaaaga
     4381 cgaaatcagc agttgaagtc gcaacaatga tcaaatcgat ttcggacgcg gcaataccgg
     4441 cgttttcaag cgccagccgg gcggcctggg tggccagtaa cgaagtggtc atgtctgcat
     4501 cggcgatatg acgttgacgt atgcccgtgc gttcggtgat ccagacatcg gaggtttcaa
     4561 cgtttcgcgt ggcgagttca gccgcgagct cgtcgttggt cactatccgc tcaggcagac
     4621 aaccgccggt ccccacgacc ctggaataag atacagtagt gttcatctga ccccagacaa
     4681 tggctcggag cctggttcgt cgttggaaat ctccgccggc aaagccgcgg aggactgcaa
     4741 attctggcgc agacgaatgg cgctggtcgt gccggccaac aggtcgttgc ataccgcccc
     4801 atgagcgcgt tccagcgcgc atccgaatgc gtagacgtcc gccgaaccgt gacttttaat
     4861 cacgattcca cgcaggccga gcaaggcggc gccgttatag cgccggttgt cgacacgatc
     4921 gcgcaggcgg ccaagcacag gcctggccag catgcccgaa atcagagaaa gcgcattgcg
     4981 cttgaattct tcccgcatca tggtagccac
//
LOCUS       DATCXU010000411         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_682587,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000411 DATCXU010000000
VERSION     DATCXU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682587"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VIR04_09260"
     CDS             <1..200
                     /locus_tag="VIR04_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY9460977.1"
                     /translation="AGVNAAVVGLLLAALYQPVWTSAILGPADFALALVALLALMLWK
                     LPPWAVVFGCAIAGWILSVAL"
     gene            complement(216..698)
                     /locus_tag="VIR04_09265"
     CDS             complement(216..698)
                     /locus_tag="VIR04_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460978.1"
                     /translation="MKTGRMTAVFFTALIASASASALAEPTQQQLDQLSATLKASMGG
                     GMMHGGMMNNESVASTPDAGQQRANPPKMQGGTAGNDPAICATMGAGTMGNHMMHSGM
                     MHGAMAGNGMMRGRSIMPQLPPGNEKLQLKMQAEIMQKVGEILAKYADQVVVGKGDAQ
                     "
     gene            863..1852
                     /locus_tag="VIR04_09270"
     CDS             863..1852
                     /locus_tag="VIR04_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY9460979.1"
                     /translation="MNKLPTPVQVDMLESAHRTLGTEISALQALDKRLDSSFNDAVSL
                     ILSCRGRVVVTGIGKSGHIAKKIAATLASTGTPAFFMHGAEAIHGDLGMLAPQDVVLA
                     ISYSGTAHELLCILSVVKRMGGRLIAMTGHPQSELAVNADLHLNASVEHEACPLGLAP
                     TASTTVALVLGDAIAVACLEARGFSTEDFARSHPGGALGRRLLTHVRDIMRQGDALPV
                     VRANTPIAETLLEMSSKRMGMTIVVDEQKHPIGIFTDGDLRRLIERYGDIRPLTVADG
                     MSRNPKSVAPAALAVEAATLMDANRLTQVLVVDNTGLLLGALHMHDLLAAKVI"
     gene            1849..2454
                     /locus_tag="VIR04_09275"
     CDS             1849..2454
                     /locus_tag="VIR04_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY9460980.1"
                     /translation="MTLNRPAISHPAEALVLAKIAPAVIERARQVRLMAFDIDGVLTD
                     GSLWYGENGEIIKGFHSLDGYGLQLLRENGILVALVTGRQGPIVARRAADLGISAVLQ
                     GVRDKAQALVALAQENGCRMDQIGYMGDDLIDLAALQRVGFAASVPNAPTYIAQAVHW
                     VASRPGGSGAVRECCDLILAAQGRLGASFAPNALTRSAAIQ"
     gene            2459..3085
                     /gene="lptC"
                     /locus_tag="VIR04_09280"
     CDS             2459..3085
                     /gene="lptC"
                     /locus_tag="VIR04_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY9460981.1"
                     /translation="MKERLPTFIAITLLAILVAGTWWAAGYAQRSVQIDPPPRMTHDP
                     DSWADDFVMVRTDPAGVAANRLEGKTMQHYPDDDSYAITAPRAIGQQPGSPITIGTSE
                     TAVMDRSGSRIVMTGNAHLHRLPGQDGKVLDVKSRQLTLLPDKDVVFTNLPALVVNGN
                     STMNGNGMHYDNRTRKLQVFSATDVKISGQDSVKNRPSAKTDQIKKKP"
     gene            3082..3741
                     /gene="lptA"
                     /locus_tag="VIR04_09285"
     CDS             3082..3741
                     /gene="lptA"
                     /locus_tag="VIR04_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY9460982.1"
                     /translation="MKPFHSILPASWLTALLIACACGLAQTAALAQPTGKASASQTKA
                     AQPAAAKKPVEEPDTTILSDSLNYDDVKKESTFTGNVIMTRGLMTLHSDKLVLREDAE
                     GFQYGTATVGKDKLVYIRQENPEKFEVVEAKGLRAEYDGKTNEIDMIGKAIVTRFVCG
                     KPFDTIKGERVKYFEKSSTYQAFGGPDSAAKDGRVRSVTRPRAQADAAAATCREKSAK
                     H"
     gene            3747..>4047
                     /locus_tag="VIR04_09290"
     CDS             3747..>4047
                     /locus_tag="VIR04_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9460983.1"
                     /translation="MFHSPQPHPGKTRALNAAHGSLQAVGLRKTYGKRTVVHDVSLSV
                     DSGEVVGLLGPNGAGKTTSFYMIVGIIPADAGRIVIDDIPITGMPIHKRARLGLSY"
BASE COUNT          869 a         1242 c         1141 g          795 t
ORIGIN      
        1 tggcgggcgt gaatgccgcc gttgtaggcc tgctgctggc ggcactttat cagccggtat
       61 ggaccagcgc cattcttggc ccggcagact tcgcattggc tcttgtcgca ctgcttgcac
      121 tgatgctgtg gaaactgccg ccgtgggcag tggtgttcgg gtgcgcaatt gccggatgga
      181 tactaagcgt tgctttgtag gacaaaatgc cgcgattact gcgcatcgcc cttgccaacc
      241 acgacctggt ccgcatactt ggcaaggatt tcgcccactt tttgcatgat ctcggcctgc
      301 attttcaact gcaacttctc gttgcccggc ggcaattgcg gcattatcga acgacctctc
      361 atcatgccgt tgcccgccat ggcgccatgc atcatgccac tgtgcatcat atgattgccc
      421 attgtccctg cgcccatcgt tgcacatatc gccgggtcat ttcccgccgt gccaccctgc
      481 atcttgggcg ggttcgcccg ttgctggcct gcatccggcg tactggcaac tgattcattg
      541 ttcatcatgc cgccgtgcat catgcccccg cccatgcttg ctttcagagt ggcgctaagc
      601 tgatcaagct gttgctgggt gggttccgca agcgccgatg cgcttgcgct cgcaataagc
      661 gctgtaaaaa atacggctgt catccgcccg gttttcatgt cggtccccca gatcaattca
      721 agttgtgcga acgttcgcca caacgaatta gtctatcctc cgccgcgggc cattacaagg
      781 gctgctggaa ggcccgatac gcggttgagg cagatgaatg taggctagaa ttcaatgaag
      841 ccaaatgaaa atatgatgaa ccatgaataa actgcccacc ccggtccagg tcgatatgct
      901 cgagtccgcg catcgcactc ttggcactga aatcagcgcg ttgcaggcgc tggacaagcg
      961 gctggacagc tcttttaacg atgccgtgtc gctcatactg agctgtcggg gccgggtcgt
     1021 cgtcacggga atcggcaaat cgggccacat cgcaaaaaaa atagccgcca ccctggcctc
     1081 gaccggtacg ccggcgtttt tcatgcacgg cgccgaggcc atccacggcg atttgggcat
     1141 gcttgcgccg caggatgtcg tgctggccat ttcctactcc ggaaccgccc acgaactgct
     1201 gtgcatactg tcggtggtaa agcgcatggg cggccggctc atagcgatga ccggccatcc
     1261 gcaatcagaa ctggcggtaa atgccgatct gcatttgaac gcctcggttg aacacgaagc
     1321 gtgcccgcta ggtcttgctc ccaccgccag cacaaccgtc gcccttgtgc tgggcgacgc
     1381 catagccgtg gcctgcctgg aagcacgcgg attcagcacc gaagacttcg cacgctcgca
     1441 ccccggcggc gcgttggggc gccggctgct gacacatgtt cgggacatca tgcgccaggg
     1501 cgatgccttg cctgtcgtgc gcgccaatac gcctatcgcc gagacgctgc tggaaatgtc
     1561 gtcaaagcgg atgggcatga ccattgtggt cgacgagcaa aagcatccga tcggaatttt
     1621 taccgatggc gacctgcggc ggctgattga acgctacggc gacatccggc cgctgaccgt
     1681 agcggacggc atgtcgcgca accccaaatc cgtcgccccc gcggcgttgg cggtcgaagc
     1741 ggcgacgcta atggatgcga accgattgac ccaggtgctt gtcgtagata atacggggct
     1801 gctattaggg gctctgcaca tgcacgacct gcttgccgca aaggtaatct gacattgaac
     1861 cgacccgcca tctcccatcc cgccgaagcg ctcgtgctgg ccaaaattgc accggccgtg
     1921 atcgagcgcg cgcgccaggt tcggctgatg gcctttgaca ttgacggagt tttaacagat
     1981 ggctcccttt ggtacggtga aaacggcgaa ataatcaaag gttttcactc ccttgacgga
     2041 tacggcctgc agcttttgcg cgaaaacgga attctcgtcg cgctggtcac cggccgtcaa
     2101 ggcccgatcg tggcgcgccg ggccgctgac ctgggcattt cagccgtact tcagggcgtg
     2161 cgcgacaaag cgcaagccct cgtcgccctg gctcaggaaa acggttgccg gatggatcaa
     2221 atcggctaca tgggcgatga cctcatcgac ctggccgcgc ttcagcgcgt cgggttcgcg
     2281 gccagcgtgc cgaatgcgcc cacgtatatt gcacaggctg tccactgggt cgcatcgcgc
     2341 cccggcgggt cgggcgccgt gcgcgaatgt tgcgatctga tcctggcggc gcaaggccgg
     2401 ctcggcgcca gctttgcgcc aaatgccctg acaagaagcg ccgccatcca gtaagatcat
     2461 gaaagaacga cttccgactt tcatcgccat tacccttctc gccattctgg ttgccggaac
     2521 gtggtgggcg gccggctatg cccagcgatc ggttcaaatc gatccgccgc cgcgcatgac
     2581 ccacgacccc gattcgtggg ccgacgactt cgtcatggtc cgcaccgatc cagcgggcgt
     2641 tgccgccaac cgcctggaag gcaaaacaat gcaacactac ccggacgatg actcctacgc
     2701 catcacggcg cccagagcga taggacagca accgggttcg ccgattacca ttgggacatc
     2761 ggaaacggcc gttatggatc gcagcggatc cagaatagtt atgacgggca atgcccactt
     2821 gcatcgtctg ccgggccagg acggaaaagt tctcgatgta aaaagcaggc aattgacgtt
     2881 actgcctgat aaagatgttg tctttacaaa cttgccggct ttagtggtta acgggaactc
     2941 gaccatgaac ggcaacggca tgcattacga taacaggacc cggaagctgc aggtattttc
     3001 agcgaccgac gtaaaaattt cgggccagga ctccgtaaag aatcgaccca gcgctaaaac
     3061 cgatcaaata aaaaagaagc catgaaacca ttccattcca ttctaccggc aagctggctc
     3121 acggcgctcc ttatcgcctg tgcctgcggc cttgctcaaa cggcggcgct ggcgcaacca
     3181 acaggcaagg cgtccgcatc ccagacaaaa gcggctcagc ccgccgccgc caaaaaaccg
     3241 gtggaagaac ccgatacaac cattttgtca gacagtctga actacgacga tgtgaaaaaa
     3301 gaaagcacgt tcaccggcaa cgtaatcatg acgcgcggtc ttatgacctt gcactccgac
     3361 aagcttgtcc ttagggagga cgccgagggt tttcaatacg gcacagccac cgttggcaaa
     3421 gacaagctgg tttacatacg gcaggaaaac ccggaaaaat tcgaagtcgt cgaagccaaa
     3481 ggtctgcggg ccgaatacga cggcaagacc aacgagatcg acatgatcgg caaagccatc
     3541 gtgacgcgat tcgtttgcgg caaacccttc gatacgatca agggcgaacg cgtcaaatat
     3601 ttcgagaaat ccagcaccta ccaggccttc ggcggacccg attcagcggc taaagacggc
     3661 cgcgtccggt cggtcacccg gccacgcgca caggccgacg ccgcggccgc cacatgccgc
     3721 gaaaaatcgg caaaacactg agccccatgt ttcattcccc tcagccgcat cccggcaaaa
     3781 cgcgcgcgct caatgccgct cacggtagct tgcaggctgt gggattacgc aaaacctacg
     3841 gaaagcgcac tgtcgtgcac gacgtttccc tatccgtgga tagcggcgaa gtcgtgggct
     3901 tgctgggccc gaacggggct ggcaaaacca ccagttttta catgattgtc ggcatcattc
     3961 ccgccgacgc ggggcgtatc gtcatcgacg acataccgat caccggcatg cccatacaca
     4021 agcgggcgcg cctggggctg tcatatc
//
LOCUS       DATCXU010000412         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_513892,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000412 DATCXU010000000
VERSION     DATCXU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513892"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VIR04_09295"
     CDS             <1..684
                     /locus_tag="VIR04_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9460984.1"
                     /translation="DADREIMLAGISHDLRTPLARMRLEIEMSSVSDEARQAIDEDLA
                     QIDHSIGQLMEYARPAGVLPEQGIDISRVLRDIFERERSHTESLGGTLTASIDPGLYA
                     KIAELDLKRVVGNLIENARRYGRSPSDNRVYIQISAREEGASLAIEVADKGTGIAESD
                     VQRLLRPFSRGESARTGVSGAGLGLAIVERLLGQVGGKLKLITRGSEGLVARIEIPKA
                     RIRNYQLDS"
     gene            752..1300
                     /locus_tag="VIR04_09300"
     CDS             752..1300
                     /locus_tag="VIR04_09300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY9460985.1"
                     /translation="MKTVGDKLEPFKVIGVKPGFNHHEENGVSAFEDITESSFPGKWK
                     IIYFYPKDFTFVCPTEIVGFNGLAKDFEDRDAVLMGGSVDNEHVKLAWRREHPDLSKL
                     GHYQFADSTGALVDQLGVRDKTEGVALRATFIVDPDNVIQHVSVNNLNVGRNPEEVLR
                     ILDGLQTDELCACGRTVGGETL"
     gene            1393..1911
                     /locus_tag="VIR04_09305"
     CDS             1393..1911
                     /locus_tag="VIR04_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9460986.1"
                     /translation="MEFLAALKNLIPDWAKDIRLNLDGTIARSSLKPEDAIGVALAAA
                     YAARSNTLITAFKSGLSDSDATGALTAAALMGMNNAYYPYVEMAGDEQLKTMQPQLRM
                     NAYGTHGGIDKTRFELFSLAASIIGKCPFCIKSHYELLKKEGFTTEQLRDAGRIAAVV
                     NAAAQVLSAEGK"
     gene            complement(1884..>2073)
                     /locus_tag="VIR04_09310"
     CDS             complement(1884..>2073)
                     /locus_tag="VIR04_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY9460987.1"
                     /translation="MGPYVPAMKAAIAADLGVPPQRINIKAKTGEGLGYVGRKEGIAA
                     SVVALCARADYLPSADST"
BASE COUNT          499 a          580 c          567 g          427 t
ORIGIN      
        1 gatgccgatc gggaaatcat gctggccggg atctcccacg atctgcgcac gcccttggcg
       61 cgcatgcgtc tggaaattga gatgagcagc gtctccgacg aggcgcgaca agccattgac
      121 gaggatctgg cgcaaatcga ccacagcatc ggacaactga tggagtacgc gcgacccgct
      181 ggcgtgcttc ccgaacaggg gatcgacata tcccgggttt tgcgggacat cttcgaacgc
      241 gagcgtagcc acactgaatc actgggcggg acgctaaccg cctcgataga cccgggcttg
      301 tacgctaaaa tcgcagaact cgacctcaag cgtgtggttg gcaatctgat tgaaaatgcg
      361 cgccgctacg gccgatctcc ttcagacaac cgggtctaca ttcagatttc agcgcgggaa
      421 gaaggggcct ctttagccat tgaggtggct gacaagggaa ccggcatcgc cgaatcggat
      481 gtccagcgcc tgttgcgccc cttctcgcgc ggcgagtcag cgcgtactgg cgtcagcggc
      541 gcaggtctgg gtctggccat tgtggaacga ttgttgggcc aggtcggcgg aaaactcaaa
      601 ctcataaccc gcggctccga ggggcttgtc gcgcgtatag aaatacccaa agctcgtatt
      661 aggaattatc aattagacag ctagttttaa tagcgtacaa ttacttttat cgggcgcatg
      721 cctctctcca cctacttaat caggagctca gatgaaaaca gttggcgata aattagaacc
      781 cttcaaagtg atcggcgtta aacccggatt caaccatcac gaggaaaacg gggtgtccgc
      841 ctttgaagac atcaccgaaa gctcatttcc cggcaaatgg aaaataattt acttctaccc
      901 caaagacttc acgtttgtat gcccgacgga aatcgtgggg ttcaatggcc tggccaaaga
      961 ttttgaagat cgcgacgcgg tcctcatggg cggttcagtt gacaacgaac atgtcaagct
     1021 ggcatggcgg cgtgaacacc ccgacctaag caagttgggc cactaccaat ttgccgactc
     1081 gaccggtgcg ctggtcgacc aactgggcgt gcgcgacaag actgaaggcg ttgcattgcg
     1141 cgccacattc atcgtcgatc ctgacaacgt cattcagcac gtgtcggtca ataacctcaa
     1201 tgtgggccgc aaccccgaag aagtcttgcg catactcgac ggtcttcaaa ccgacgaact
     1261 gtgcgcgtgc ggccgcacgg taggcggcga aacgctgtaa ttcacaggcc cggttcgtcc
     1321 gggctttgtt ggaacttcat aatagataaa atctattaag tagtcatatt taataaccaa
     1381 agaggactaa aaatggaatt tctggcagcg ctcaaaaatc ttattccaga ctgggccaaa
     1441 gacattcgcc ttaatctgga cggcacgatc gcgcgttcat cgcttaaacc ggaagacgcc
     1501 atcggagtgg ccctggccgc cgcctatgcg gcgcgcagca atacgctgat cacggcattc
     1561 aaaagtggtt tgtcggactc tgacgcgaca ggcgcattaa ccgccgccgc tctgatgggc
     1621 atgaataatg cgtattaccc atacgtcgag atggccggcg acgaacagct taagaccatg
     1681 cagccgcaat tgcgcatgaa tgcctatgga acccatggcg gaatcgacaa gactcgcttc
     1741 gaattgtttt ccctggcggc atcaataatc ggcaaatgcc cattctgcat caaatcgcac
     1801 tatgagctgc ttaaaaaaga aggctttaca accgagcaat tgcgcgacgc cggacgtatt
     1861 gcggcggtgg ttaacgcggc cgctcaggtg ctgtcggctg aaggcaaata atcggcgcgg
     1921 gcgcacagcg ccacgaccga agccgcgatg ccctctttgc ggcccacata gcccagaccc
     1981 tctcctgttt tggccttgat attgatccgt tgcggcggca cccccaggtc ggcggcaata
     2041 gccgccttca tggcgggaac gtacggcccc atc
//
LOCUS       DATCXU010000413         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_436991,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000413 DATCXU010000000
VERSION     DATCXU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436991"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VIR04_09315"
     CDS             <1..683
                     /locus_tag="VIR04_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY9460988.1"
                     /translation="AAPRQWLNGGFSCSQARALTRRLGAELARLHQIPASDRRLAFMV
                     PPAQPPALNRVRQYRDALAVIPEPHPVLEWSLNWLQDQAPDSNAVVLCHGDFRTGNYM
                     VDKGRLTAILDWEFAAWSDPCEDLGWLCSRSWRFGHNDKEVGGVGDKKDLFSGYDELA
                     KQSIDPDKVLYWEVMALMRWAVIALQQAQRHLSGEQASLELALTGRLLPQIEFDLLTQ
                     IHEIEDGR"
     gene            680..1045
                     /locus_tag="VIR04_09320"
     CDS             680..1045
                     /locus_tag="VIR04_09320"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEY9460989.1"
                     /translation="MINQPRGDELLAVARRTLLDGLLPLIPPDRAYEARMIANAMAIA
                     ARELARCKNVDDETAEGIAEFYRAIGMDPSVIASEQSLADQIRKRRIDSVHSRLLHDL
                     LLAVTRAKLAISNPKYLDQ"
     gene            1059..2681
                     /locus_tag="VIR04_09325"
     CDS             1059..2681
                     /locus_tag="VIR04_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY9460990.1"
                     /translation="MSTPYQFGLDKRDANYSALTPISFLQRSATIFPHKKAVIDDDMS
                     LTYLQFYRRCRSMASALREMGIRAGDTVAIMGFNGHELLESHYSVPMIGAVLNALSTR
                     LDVESLRFILEHGEARVLFYDTVFEDLVGAAVRQLESPPLLISIDRVAQQGDKLASLS
                     YEQLVAFGDPDFAWTKVSDEWDAITLNYTSGTTGNPKGAVYHHRGAYLSAMSNAMAFN
                     MNPDTVYLWTLPMFHCNGWCYTWAITAVGGTHVCLRKVDADAVLARIEAHHVTHMCGA
                     PVVLNLLLNDFHNKSKSLATPVQFALGGAAPPSPVISRAQKIGFTITHLYGLTESYGP
                     SMLCVWQSDWGGLPVDDLASKMARQGVGNFAIDDVQVANLTDRSFVPPDGKALGEILL
                     KGNTLMKGYLKNQAGTQEAFRRGWFHTGDLAVVHPDGYVEIKDRAKDIIISGGENISS
                     QEIEDVLYRHPMVLEAAVVALPDEKWGEVPCAFVCLKDNAPALDERDFIEFCRSCMAS
                     FKVPKKVIFGELPKTATGKIRKNILRQQLANR"
BASE COUNT          633 a          759 c          855 g          675 t
ORIGIN      
        1 cagcggctcc gcgccagtgg ctcaacggcg gcttcagttg cagccaggca cgcgcgctga
       61 cccggcggct tggcgccgag ttggcgcggt tgcatcaaat tccggcgtcc gaccggcgcc
      121 ttgcctttat ggtgcctccg gctcagccgc cggcattaaa tcgcgtgcgg cagtatcgcg
      181 atgcgctggc cgttataccc gaacctcacc cggtgcttga gtggtcattg aactggctgc
      241 aggatcaggc tcccgacagc aatgcggtcg tgttgtgcca cggcgacttc cgcacgggca
      301 actatatggt ggataaagga cgactaacgg cgatattgga ctgggaattt gccgcatgga
      361 gcgacccctg tgaagatcta gggtggctgt gttccagaag ctggcgcttc ggccataacg
      421 acaaggaagt gggcggcgtt ggcgacaaga aggatctttt cagcggctat gacgagctgg
      481 ctaaacaatc gattgatcct gacaaggttt tgtattggga ggtcatggcg ctgatgcgct
      541 gggccgtcat tgccttgcaa caggctcagc gccatttatc cggcgaacaa gcgtcacttg
      601 aactggcctt gacagggcgg ctgttgccgc aaattgaatt cgatttgttg acgcaaatcc
      661 atgagataga ggatggacga tgattaacca gcctcgtggc gatgaattgt tggcggtggc
      721 ccggcgcacg ctgctcgacg gtttgttgcc gctgattccg cctgacagag cctacgaggc
      781 gcggatgatc gccaacgcca tggcgatcgc ggcacgcgaa ctggcccgat gcaagaacgt
      841 tgacgacgag accgccgaag gaatcgcgga attttatcgc gccatcggaa tggatccttc
      901 agtcattgca tccgaacaat ccctggccga tcagatcaga aagcggcgta tagattctgt
      961 tcattcgcgg ctattgcacg atttgttgct ggccgtcacg cgtgccaaac tggccatcag
     1021 caacccgaag tatttagatc aataacgcag gcgatcatat gtctactccc tatcaattcg
     1081 ggctcgataa gcgggatgcg aattactcgg ctttaacgcc catcagtttt ctgcaacgtt
     1141 cggcaaccat atttccgcac aaaaaggctg tgatcgacga tgacatgagc ctgacttacc
     1201 tgcagtttta tcgtcgttgc cgttcgatgg cttcggcgct gcgcgaaatg ggtattcgcg
     1261 cgggcgatac agtcgccatc atgggcttca acggccacga acttcttgaa agtcattatt
     1321 cagttccgat gatcggcgcc gtgctgaacg cgctcagcac gcggctggat gtcgaatcgt
     1381 tgcgtttcat tcttgaacat ggcgaagcgc gtgttttgtt ttacgacacg gtgttcgagg
     1441 atctggtcgg agcggcggtg cggcaactcg agtccccacc gctattgatc agcattgatc
     1501 gcgttgctca acagggcgac aagctggcgt ctctgtctta cgagcaactg gtcgcattcg
     1561 gcgatcccga ttttgcctgg accaaagtca gcgacgaatg ggacgccatc acgcttaatt
     1621 acacctcggg tacgactgga aatccgaagg gtgcggtata ccatcatcgc ggcgcatatc
     1681 tgtcggccat gagcaatgcc atggccttca atatgaaccc ggacacggtc tacctgtgga
     1741 ccttgcccat gtttcattgc aacggctggt gctacacgtg ggcgatcact gccgtgggcg
     1801 gcacgcatgt atgcctgcgc aaagttgacg ctgacgcggt gctggcgcgc attgaagcgc
     1861 accatgtcac gcatatgtgc ggcgcacccg ttgtgttgaa cttgctgctt aatgattttc
     1921 ataacaagag caagtcgctg gccacgccgg tgcagtttgc attgggcggc gcggcgcccc
     1981 ccagtccggt cattagccgt gcgcagaaaa ttggattcac catcacccat ctgtatggac
     2041 tgaccgaatc gtacggcccc tccatgttgt gcgtatggca gagcgactgg ggtggtttgc
     2101 cggtggatga tctggcgtca aaaatggcgc gccagggggt cggcaatttt gcgatcgacg
     2161 acgttcaggt ggcaaacctg actgaccgaa gcttcgtgcc gcccgatggt aaggctctgg
     2221 gcgagattct gcttaagggc aataccctga tgaaaggcta tttgaaaaat caggcgggca
     2281 ctcaggaggc attcaggcgt ggctggtttc atacgggcga cctggcggtc gtgcatcccg
     2341 acggctacgt cgaaataaaa gaccgggcca aggatattat tatttcgggc ggcgaaaata
     2401 tatcgagtca ggaaatcgaa gacgtgctgt accggcatcc tatggtgctc gaggccgcgg
     2461 tggtggcgct gcccgatgaa aaatggggcg aggttccgtg cgcgtttgtt tgcctgaaag
     2521 acaatgcccc cgcccttgat gagcgcgatt tcatcgaatt ttgccgatcg tgcatggcgt
     2581 cgttcaaggt ccctaaaaag gtcattttcg gcgagcttcc gaaaacggca acagggaaaa
     2641 ttcgaaagaa tattttaagg caacagttgg caaatcgcta atttcaggag ggcaggcaat
     2701 cgattatcga tgctttattt ttggaagaag aagtcggagt tcacagtgca tttacggaag
     2761 ttttaataga accataatcc taagaggagt ctcccatcat gtttaaaact gttttcaggc
     2821 atcccttgct ggtactcgcc atttcgggtg ttttcagttt caatccggct agcgcggcgg
     2881 accagacacc gataaaaatc ggcggcatct accttatgtc gg
//
LOCUS       DATCXU010000414         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_97713,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000414 DATCXU010000000
VERSION     DATCXU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97713"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VIR04_09330"
     CDS             complement(<1..939)
                     /locus_tag="VIR04_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY9460991.1"
                     /translation="MGNSNNPHNALWRTAATAMLNRAEKTALALDGQGFPHWADSRTG
                     KWTTTADGDWTGGAFPGIFWLGYRMTGESRMLELARIWSRRLRPRAQLETAFKGFGFY
                     YGAALGDVLTGDKESASLALEAAESLLRQFDPALGLIPLGRDAEEAAETGRAFSSIDS
                     LQASPLLFWAARHTDELRYAECASRHTMRVLEIHCRPDGSIVQSTELDGKTGQTVRHF
                     THKGFGDSSVWGRAQAWGMLFAAMAFAQGRQDPWLAQSMASADWWLSNVPMDGVAYWD
                     FNDPAIPGAPRDTAATSIACAALLRLAELAPTAADRQ"
     gene            complement(1141..1266)
                     /locus_tag="VIR04_09335"
     CDS             complement(1141..1266)
                     /locus_tag="VIR04_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9460992.1"
                     /translation="MIGSVKLFWKGRPHRTMSIAAGLAIYNMIEAWTHVALGRIT"
     gene            1265..2410
                     /locus_tag="VIR04_09340"
     CDS             1265..2410
                     /locus_tag="VIR04_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9460993.1"
                     /translation="MPHWLAEQHTAIRNAVRRFAESEIAPVSEELWADEKFPYAIWKN
                     MAKLGFTGLPYPEEYGGGGGEWLSFAIVLEELARVDCAVANSVMANSTVASLLSKYGT
                     HEQKTAWLAPILDGRQVGAIGLSEPNAGSDAANISTRATLEEGQWIIDGAKTFISNSG
                     TDVTGPVVIAAVTGTRPDGRKEISNFIVPDGTPGFHYGRKLRKIGWRASSTYELFFQH
                     CAIPAANLLGERGAGLRQTLAQISTGRIFIGVLALGILQGSLDRSIDYMKSRHAFGKP
                     ISEFQALQFKIADMATHAQAARLMLYDAAALKDADQPFNQQASMAKLFATERAMEAAH
                     QALQIHGGYGVMSEFPVSRAFGDAKVLEIVEGTSEIQRLIISRELLA"
     gene            2433..>3078
                     /locus_tag="VIR04_09345"
     CDS             2433..>3078
                     /locus_tag="VIR04_09345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9460994.1"
                     /translation="MLEGIKVLSFTHFLQGPAAAQMLADVGADVIKIEPPSGAFERNW
                     SGLDAFVEGVSVFFLLGNRNQRSISLNLRDERAKEIVWRLIREADVLIENYRPGVLQK
                     MGFGYEDVHAINPRLVYCSCTGYGSSGPYSKRPGQDLLLQAMSGMTMLSGQADSPPTP
                     FGAAIVDQHAAALGAFGVLAALQARERTGTGALVESNLLNAALDLQTEPLTYYMN"
BASE COUNT          661 a          914 c          883 g          620 t
ORIGIN      
        1 ttggcggtcg gcggctgtcg gagccagttc agccaggcgc aataaagcgg cgcaggcaat
       61 cgacgtggcg gccgtatcgc gcggtgcgcc gggaatggcc ggatcgttga agtcccaata
      121 agcaacacca tccatgggaa cgttcgaaag ccaccagtcg gccgacgcca tgctttgcgc
      181 gagccatggg tcttgccggc cttgcgcaaa cgccattgcc gcaaaaagca tgccccacgc
      241 ctgggcacgt ccccatacgc tggagtcgcc gaaaccctta tgagtgaaat ggcgaactgt
      301 ctgaccggtc ttgccgtcga gctccgtgga ttgcacgatc gaaccatcgg ggcggcaatg
      361 gatttcaagt acgcgcatgg tgtgccggct tgcacattcg gcgtaacgca attcgtcggt
      421 atgccgcgcg gcccaaaata acagcgggct ggcttgcagc gaatcgatgc tgctgaatgc
      481 gcgaccagtt tccgccgctt cctcggcatc gcggcccagc ggaatcagac cgagcgccgg
      541 gtcaaactgc cgcaacagcg attcggccgc ctcgagcgcg agactggccg attccttatc
      601 gcccgtcagg acgtctccca gtgccgcacc gtaataaaaa ccgaaacctt tgaaagccgt
      661 ttccagttgg gcccgcggac gcaaccttcg gctccagatc cttgccagct caagcattcg
      721 ggattcgccc gtcattcgat agcccagcca gaaaatgccg ggaaatgcgc caccggtcca
      781 gtctccgtcg gcggtggtgg tccatttgcc ggtgcgcgag tcggcccaat gaggaaagcc
      841 ctgtccatcc agggcaagcg ccgtcttttc cgcacgattc agcatggcgg tcgcggccgt
      901 tcgccacaag gcattgtggg gattgttcga atttcccatc gagttccttt gaagaccggc
      961 ccagggatcg tccaacgccc cgccagtatg gtttttgaca cttcattatc gctgaacgaa
     1021 aaaagaaacg ttaggactga ccaacgaatt tcttgcactt tccaatgtgc attctcttga
     1081 cgtccctgtc aggaatacgc caggaccggc cgtcggttca acgtacaaaa atccaactat
     1141 tcatgtaatt cgtcctaacg cgacatgcgt ccacgcttct atcatgttgt aaatggcaag
     1201 accggcggca atcgacattg tgcgatgcgg ccggcctttc cagaacaatt taacggagcc
     1261 gatcatgcca cactggctag ccgaacaaca cactgccatt cgaaacgccg tgcgccgctt
     1321 cgcggaaagc gagatcgcgc cggtgtccga agagctttgg gctgacgaga agtttcccta
     1381 cgcgatttgg aagaacatgg caaagctggg ctttacaggg ttgccttatc cggaggaata
     1441 cggcggaggc ggtggcgaat ggctgtcgtt cgcgatcgtg ctcgaagaac tggcccgcgt
     1501 ggactgcgcc gtcgccaact cggtcatggc caattccacc gtggcaagcc tgttgtccaa
     1561 atatggcacc cacgagcaaa aaactgcctg gctggcgcct attcttgacg gccgccaggt
     1621 cggcgcaatt ggcttgtccg aacccaacgc aggttcggat gccgcaaaca tctcgacccg
     1681 cgcgacgctc gaagaaggac agtggatcat cgacggtgca aaaactttca tcagcaactc
     1741 cggcactgac gtgaccgggc ctgttgtgat cgccgccgtt accggaacac gtcctgacgg
     1801 tcgcaaagag atctccaact tcatcgtgcc cgacggcacg cccggctttc actatggccg
     1861 caagttgcgc aagattggct ggcgcgcttc gagcacgtac gagctattct tccagcactg
     1921 cgccataccg gctgccaatt tgcttggcga acggggcgcc ggcctgcgcc agacacttgc
     1981 gcagatcagc accggccgca tctttatcgg cgtactggca ctgggaattt tgcaaggatc
     2041 gctggatcgc tcaatcgatt acatgaaaag caggcacgca tttggcaaac ccatttccga
     2101 gtttcaggcc ttgcaattca agatcgccga catggcaacg cacgcgcaag cggcacgact
     2161 gatgctttat gatgccgccg ccctgaaaga tgcggaccag cccttcaatc aacaagcgtc
     2221 catggccaaa ttgttcgcca ctgaacgcgc catggaggcg gcccaccagg cgcttcagat
     2281 acatgggggc tacggggtga tgagcgaatt tcctgtctcg cgcgcgttcg gcgatgcgaa
     2341 agtactggaa atcgtcgagg gcacatcaga aattcagcga ttaattattt cgcgcgagct
     2401 gctggcgtaa ttcagcgaca tggagaaaaa tcgtgctgga aggcatcaag gtattgagtt
     2461 tcacgcattt tttgcaggga cccgccgctg cacagatgct ggctgacgtc ggcgcggacg
     2521 taatcaagat cgaaccaccc agcggcgctt tcgagcgcaa ctggtcaggg cttgacgcgt
     2581 tcgtcgaagg cgtcagcgta tttttcctgc tcggcaaccg caaccagcgc agcatctcgc
     2641 tgaacctgcg cgatgaacgc gcgaaagaga tcgtctggcg tctgatccgc gaagcggatg
     2701 tattgatcga gaattaccgg cccggtgttc tgcaaaaaat gggattcggc tatgaggacg
     2761 ttcatgcgat caatcccagg ctcgtttact gctcgtgcac ggggtacggt tcgtcgggcc
     2821 cttactcaaa gcgccccggc caggacttgt tgctccaggc catgagcggc atgaccatgc
     2881 tgtccggcca ggctgattcg ccccctacac cgtttggtgc ggctattgtc gaccagcacg
     2941 ccgccgcgct cggtgcgttc ggagtccttg cggcattaca ggcgcgcgaa cggactggca
     3001 cgggcgcgct ggtggaaagc aatttgttga atgcagcgct tgatctgcag acggaaccgc
     3061 ttacatacta tatgaaca
//
LOCUS       DATCXU010000415         5287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_672987,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000415 DATCXU010000000
VERSION     DATCXU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672987"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VIR04_09350"
     CDS             complement(<1..897)
                     /locus_tag="VIR04_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY9460995.1"
                     /translation="MASRPLQIVFLDRETISPRTVLRTPSFAHELTVHDRTSRDQVGA
                     RIAAADIVITNKVPIDRVALNGAPRLKMVAVAATGTDIVDLAACAAHGVVVSNIRGYA
                     VNTVPEHTFALIFALRRSIVAYRESVKAGRWHDSQQFCYFDYPIRDLTGSKLGIIGDG
                     VLGQAVARIGRALGMNVSFAARKGHPRSGALYTPFEDILSQSDVITLHCPLNAQTRNM
                     IDAAEFAQMARKPLLINTARGGLVNEQALAQALRSGQIGGAGFDVTAPEPPDNQSVLV
                     QLLDLPNFILTPHVAWASEEAVQ"
     gene            1044..1739
                     /locus_tag="VIR04_09355"
     CDS             1044..1739
                     /locus_tag="VIR04_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9460996.1"
                     /translation="MPEQVALIVGAGPGLSAALARRCAADGMRVGLVARNTEKLDGLV
                     AEIDARAFACDVTNTANVKHLFTRVENELGVPDLVVYNPSARAKGSIVDLDPQEVEKA
                     LMVTCFGGFLVAQQAVKLMLQRGQGTMLFTGASASVKGYANSAPFAMGKFGLRGLAQS
                     MARELAPKGIHVAHFVIDGSIAAPSTPGVDPSAPDDKLNPDVIAANYLAIHHQHRSAW
                     TWEIELRPWLEHF"
     gene            1777..3633
                     /locus_tag="VIR04_09360"
     CDS             1777..3633
                     /locus_tag="VIR04_09360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY9460997.1"
                     /translation="MSVTSPPKRYPIKDFFRNPDRGYFRLSDDGAQLGFMEPVSIDGH
                     PPRMNIFVQTLEGSTPTGNPRRLSSETARDISNFFWKGSDTVLYEKDFNGDENFHVLA
                     VNAGTGVLTDLTPFSGVRASVQDDLEDDPDHILISHNQRNPEVFDVYRANIRTGQLTL
                     VAENPGNIVGWQTDHVGRVRMGIVSDGLHTTLLYRDDENQAFRPIITTDFRTTVSPEF
                     FTFDNRRFYALSNRSRDCLALVTIDPVAPDREHVVFSADHVDLDGAEYSRLRHVLTMA
                     SYQTDKPQRHFFDDLAAQRFKNVSEKLPGYEVILQNSTRDEKKYIVAAYNDRTPGSRY
                     IYDANNNTLDKLADINPDLPESDMACVQPIQYQSRDGLTIHGYLTLPPGRVASGLPCV
                     VNPHGGPWARDGWGFNPEVQFLANRGLAVLQMNFRGSTGYGRRFWEASFGEWGLSMQD
                     DITDGVHWLIDSGIADPKRIAIYGGSYGGYATLAGATYTPDLYAAAVDYVGVSNLLTF
                     MNTIPPYWKPMLEKMHSMVGHPEADHERLVATSPALNADKIKVPLLIAQGAHDPRVNK
                     DESDQMVAALQARGVAVEYIVKEDEGHGFHNDENKFEFYERMENFLTQHLKL"
     gene            3642..4250
                     /locus_tag="VIR04_09365"
     CDS             3642..4250
                     /locus_tag="VIR04_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY9460998.1"
                     /translation="MKLYYIRGACPLVPHIVLEWIGKPFEAQEVSRTEVKEPKFLAMN
                     PTGQVPVLADGDLTLTQNASILEYLAELNPEARLLGETPRERAETRRWFGFCNSDLHR
                     TFSIVFAAPSYIAGAEGQAELIANTKKKVIGFFHIADKQLKGKKWLTGSRSIADPYLY
                     TLMRWAKAKEMDLSDCVNLQAFFAHMEADPGVRAAVKAQGLN"
     gene            complement(4288..4989)
                     /locus_tag="VIR04_09370"
     CDS             complement(4288..4989)
                     /locus_tag="VIR04_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9460999.1"
                     /translation="MLKLENVHTYYGAIQALNDVSVEVNRGEIVTLIGANGAGKTTLL
                     MTVCGSPRAHSGRITFEDEDISATDTHTIMRKGIAIAPEGRRIFPDLTVAENLKMGGF
                     FLTKGEIAESLDHNYDLFPILKERANQRAGTMSGGEQQMLAIGRALMARPKLLLLDEP
                     TLGLAPLIIAQIFDIIRTIRDEGVTIFLVEQNANKALQVADRGYVLETGRVVLADTGA
                     HLLVNSDVRKAYLGA"
     gene            complement(4990..>5287)
                     /gene="livG"
                     /locus_tag="VIR04_09375"
     CDS             complement(4990..>5287)
                     /gene="livG"
                     /locus_tag="VIR04_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165784.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HEY9461000.1"
                     /translation="HQRRLEIARCMITKPRLLMLDEPAAGLNPQEKHDLQKLIDQLRK
                     EFSVAVLLIEHDMSLIMGVSDRILVMEHGKPVMTGLPADVRGDARVIKAYLGEE"
BASE COUNT         1205 a         1555 c         1432 g         1095 t
ORIGIN      
        1 ctgcacagct tcctcgctgg cccacgccac atgcggggtc aaaataaaat taggcagatc
       61 aagcagttgg accagaacgc tttgattatc tggtggttca ggcgctgtga catcaaatcc
      121 cgctccgccg atttgccctg accgcaacgc ttgcgccaat gcctgctcgt tgaccagacc
      181 gcctcgggcg gtattgatca aaagcggctt gcgcgccatc tgcgcaaatt cagcggcgtc
      241 gatcatattg cgcgtttgcg cgttaagcgg gcaatgcagg gtgatgacat cgctttggct
      301 caaaatatcc tcgaaaggcg tatataaagc gccacttctg gggtggcctt tgcgcgcagc
      361 aaacgacaca ttcatgccca atgcccgccc gatacgcgcc actgcctgtc ccagcacgcc
      421 gtcaccgata atgcccagct tggaaccagt gagatccctg atgggatagt caaaataaca
      481 gaattgctgc gagtcgtgcc agcgccccgc tttgactgat tcgcgatacg ccacaatgct
      541 gcggcgaagt gcgaaaatga gcgcaaaggt atgttcgggc accgtgttca ccgcatagcc
      601 gcgaatgttc gacaccacca cgccgtgcgc cgcgcaagca gccaggtcca cgatatccgt
      661 accggtcgcc gcaacggcca ccatcttcag ccgcggggca ccattaagcg cgacccggtc
      721 aatggggacc ttgtttgtga tgacaatatc agcggcggcg atccttgcgc cgacctggtc
      781 gcgcgaggtg cgatcatgca ccgtcaattc atgcgcgaac gacggagttc gcaagactgt
      841 tcggggcgag atggtttccc gatctaaaaa tactatttgc aaggggcggc tggccatgta
      901 agagtccttc gcttccgacg ggcggcatgc tgattttcgt tattcgcaca aaattcgaag
      961 cgggcgatgg cgcccgccgg gctattttgg cacacttgtc cgccaataca gcggattaca
     1021 atgaaattct aaggagaaaa acaatgccag aacaagttgc cctgattgtt ggagccggac
     1081 ccggcttgag cgccgcgtta gcgcgtcgtt gcgccgctga cggaatgcgg gtcggcctgg
     1141 tggcgcgcaa taccgaaaaa cttgacgggc tcgttgcgga aatcgacgca cgtgcattcg
     1201 cttgcgatgt caccaacacg gcaaacgtca aacacctgtt tacccgggtg gaaaacgagc
     1261 tgggtgttcc cgatctggtg gtctacaacc cgagcgcccg cgcgaaaggc tccatcgtcg
     1321 atctcgaccc gcaggaagtt gaaaaggcat tgatggtaac ttgtttcggg gggtttctgg
     1381 ttgcccagca ggccgtcaaa ttaatgctgc aacgcgggca aggaaccatg ctgttcacgg
     1441 gagcctcggc aagcgttaaa ggctacgcca attcggcccc gtttgcgatg ggcaagttcg
     1501 gattgcgcgg actggcccaa agcatggcgc gcgagctggc cccgaagggg attcatgtgg
     1561 cgcacttcgt cattgacggc tctattgcgg cgccatccac accgggcgtt gatccgtccg
     1621 cgcccgacga caagttgaat cctgacgtaa tcgcagccaa ttacctggcc atccaccatc
     1681 aacaccggag tgcgtggact tgggaaattg agctacgacc ttggctggaa cacttttaac
     1741 ttgcataatc cacgccaaga ctcaaggatc acgacaatgt ctgttacttc tcccccgaaa
     1801 cgttacccga tcaaggattt tttcaggaat ccggaccgcg gctattttcg cctctccgac
     1861 gacggcgcgc agcttggatt catggaaccg gtcagtatcg atgggcaccc gccgcgcatg
     1921 aatattttcg tgcaaactct ggaaggcagc acgcccaccg gaaacccgcg ccggctaagc
     1981 tccgagacgg cgcgcgacat cagcaacttc ttctggaaag gatcggatac cgtcctgtat
     2041 gaaaaggact ttaacggcga tgaaaacttt catgtgctgg cggtcaatgc cggcaccggc
     2101 gttttaaccg atctgacgcc attcagtggt gtccgggcca gcgttcagga tgaccttgag
     2161 gatgaccctg accacatttt gattagccac aatcagcgca atcccgaagt gttcgacgtc
     2221 tatcgcgcca acattcgcac gggacaattg acgctggtcg ccgagaaccc gggcaacatc
     2281 gtgggctggc aaactgacca cgtcgggcgg gtgcgcatgg gtatcgtcag tgatggcctg
     2341 cacaccaccc tgctgtaccg ggacgacgaa aaccaggcgt tccgccccat cataacgacc
     2401 gacttccgaa ccaccgtcag ccccgaattc ttcaccttcg ataatcggcg cttctatgcc
     2461 ctgagcaatc gcagccggga ctgcctggcg ctcgtgacga tcgacccggt cgcgcccgat
     2521 cgggaacatg tggtgttttc ggccgatcac gtcgacctgg acggcgccga atattcgcgt
     2581 ttacgtcatg tgctcacgat ggcgagctac caaacggata aaccgcaacg ccactttttt
     2641 gacgaccttg ccgcgcaacg cttcaagaac gtatctgaaa aactgcccgg ctacgaagtc
     2701 attctgcaaa acagcacccg tgacgaaaaa aagtacatcg tggccgccta caacgacagg
     2761 acaccgggca gccgctacat ctacgacgcg aacaacaata cgctggacaa gctggcggat
     2821 atcaaccctg atctgcccga aagcgacatg gcttgcgtcc agccaatcca gtatcaatcg
     2881 cgcgacggcc tgacaattca tggctacctg actcttccgc cagggcgtgt cgccagtggc
     2941 cttccctgcg ttgtcaaccc tcacggcggc ccatgggcac gggatggctg gggtttcaac
     3001 cccgaagttc agtttctggc caatcgcgga cttgccgtcc tgcagatgaa tttccgtggt
     3061 tccaccggct acggccgacg cttctgggag gccagtttcg gtgaatgggg tctgagcatg
     3121 caggatgaca ttaccgatgg cgttcactgg ctcatcgact cgggtattgc ggatccgaaa
     3181 cgtattgcca tttatggcgg cagctacgga ggctacgcga cccttgccgg cgccacgtac
     3241 acgcctgatc tgtacgcggc agcggtcgat tacgtagggg tatcgaacct gcttacattc
     3301 atgaacacca ttccccccta ctggaaaccg atgctcgaaa aaatgcacag tatggtcggc
     3361 catcccgaag cggaccatga acggctggtg gccacgtcgc ctgctcttaa cgccgacaag
     3421 attaaggttc ctctgctcat cgcacaaggg gctcacgatc cgcgtgtgaa taaagatgaa
     3481 agcgaccaga tggttgcggc attgcaagcc cgcggcgtcg ccgttgaata cattgtcaag
     3541 gaagacgaag gccacgggtt tcacaacgac gaaaacaagt tcgaattcta cgaacgcatg
     3601 gaaaactttc taacccagca tttgaaactg taaggaaaac gatgaaactt tattacatac
     3661 ggggcgcgtg ccccctggtc ccgcacatag ttctggaatg gatcggcaaa cccttcgaag
     3721 cgcaagaggt cagccgcacc gaagtcaaag aacctaaatt ccttgcaatg aatcccacag
     3781 gccaagttcc tgtgcttgcc gacggcgatc tgaccctgac gcaaaacgca tccatcctgg
     3841 aatatctggc cgaactcaat ccagaagcca ggctattggg cgaaacgccc agggagcgcg
     3901 ctgaaacgcg ccgctggttc ggtttctgca actccgattt gcacagaacg ttcagcatcg
     3961 tgttcgccgc cccgtcttac attgcggggg ccgaaggtca ggctgaatta atcgccaaca
     4021 cgaagaaaaa agtcattggg ttctttcata tcgccgacaa gcaattaaag ggcaaaaagt
     4081 ggctgacggg ctcgcgctct atcgcggacc cgtacctgta cactttgatg cgctgggcca
     4141 aagcaaaaga gatggacttg tcagactgcg tcaatctaca ggctttcttc gcccacatgg
     4201 aggccgatcc gggcgtgcgg gccgccgtta aagcccaggg tctgaactag acgccaaccg
     4261 cgtgccagcg ggccgaccgg ccttgggcta tgctcccaga taggctttgc gcacgtcgct
     4321 attaaccagc aaatgggcgc cggtgtcggc cagtacgacc cggccggttt ccaggacata
     4381 gccgcgatcg gccacctgca aagccttgtt ggcgttttgc tcaaccagga aaatggtaac
     4441 gccctcgtcg cgtatggttc gaatgatgtc gaaaatctgg gcgattatca aaggagccaa
     4501 acccaaagtg ggctcatcaa gcagcagcaa ttttggccgc gccatcagtg cgcgcccaat
     4561 ggcaagcatc tgctgctcgc cgcccgacat cgttcccgcg cgttgattgg cgcgctcttt
     4621 taatatgggg aacaaatcgt aattatgatc aaggctctcg gcgatttcgc ccttggtcag
     4681 aaaaaagccg cccatcttca agttctcggc cacagttaga tcgggaaaaa tgcgacggcc
     4741 ctcgggcgct atggcaatgc ccttgcgcat aatggtgtgc gtatcggttg cgctgatgtc
     4801 ctcgtcttca aatgttatgc ggccgctatg cgcgcgtggc gatccgcaca ctgtcatcag
     4861 caaggtggtt ttacccgcgc cgttcgcacc gatcaacgta actatttcac cccggttcac
     4921 ttcgactgaa acatcgttga gcgcctgaat ggcgccgtaa taggtgtgaa cattttccag
     4981 cttaagcacc tattcctctc ccagataggc cttgataaca cgcgcatcgc cacgcacatc
     5041 ggccggcaga cccgtcatga ccggcttgcc gtgctccatc accagaatac ggtcggatac
     5101 ccccataatc agactcatgt catgctcgat caataacacc gccacactga attctttgcg
     5161 caattgatca ataagttttt gcagatcgtg tttttcctgc ggattcaaac cggccgccgg
     5221 ctcgtcgagc atgagcaggc gcggtttcgt gatcatgcaa cgcgcaattt caagccgccg
     5281 ttggtgg
//
LOCUS       DATCXU010000416         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1143424,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000416 DATCXU010000000
VERSION     DATCXU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143424"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VIR04_09380"
     CDS             complement(<1..437)
                     /locus_tag="VIR04_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY9461001.1"
                     /translation="MQDLANKRIILGLTGGIACYKSAELLRRLLDHGAQIDVVMTEAA
                     THFITATTMQALSGRPVFLEAWDARMPNSMAHINLTRGADAILIAPASTDFMARLAHG
                     LADDLLSALCVAKGNCPLLIAPAMNREMWLHPATQRNVAQLAA"
     gene            complement(449..997)
                     /gene="lspA"
                     /locus_tag="VIR04_09385"
     CDS             complement(449..997)
                     /gene="lspA"
                     /locus_tag="VIR04_09385"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY9461002.1"
                     /translation="MPEAGSVSSSSPKRPGRLWIWILGAVLIVGADQLAKAWFNGHLH
                     YGERLHVMPFFDFTLLYNPGAAFSFLADGQGWQRWLFTAIALIAAGLIIHLLRRSLDQ
                     PLFCASLMAILGGAVGNVVDRIQHGHVIDFLLFYWNTWYFPAFNVADVAITCGAILLI
                     LDELRRHRRERRQAAESTSFDK"
     gene            complement(990..3827)
                     /gene="ileS"
                     /locus_tag="VIR04_09390"
     CDS             complement(990..3827)
                     /gene="ileS"
                     /locus_tag="VIR04_09390"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY9461003.1"
                     /translation="MDYRNTLNLPETPFPMRGNLPKREPGWIAQWEEKKVYAAIRAAS
                     AGRPKFILHDGPPYANGDIHIGHAVNKILKDIILKSRSMAGFDAPYVPGWDCHGMPIE
                     IQIEKKYGKNLPVDQVQSRARAYALEQIDRQRKDFKRLGVLADWDHPYLTMNFSNEAN
                     ELRCLGRILEKGYVFRGLKPVNWCFDCGSALAEAEVEYADRTDPAVYVAFKFSDKKKL
                     AHAFGLTDVDDGAAVIWTTTPWTLPANQALNVNPEIDYVLVKLTEPLGVGSVLLLARD
                     LVESCLQSWGLRGEIIAAAKGSALAGLEFIHPLADLDPGYRRKAPVYLGDYVTLDAGT
                     GVVHSSPAYGIEDFVSCKANGLTDEQIISPVMGDGHYAASLPVFGGLMIWKANPEIVK
                     ALDSVGSLLKVEQHTHSYMHCWRHKTPIIYRATSQWFAGMDRQPKSGKSLRKTALEGI
                     DNTQFFPAWGRARLHAMIANRPDWTLSRQRQWGVPMAFFVHKETGQLHPRTPELIEQV
                     AQRIEQHGIEAWQSLDPRELLGDEADQYEKNRDTLDVWFDSGTTHATVLGGKNHEFKG
                     SRGEELGWPADLYLEGSDQHRGWFHSSLLTGCMLYGAPPYKALLTHGFVVDGQGRKMS
                     KSVGNVIAPQKVSDSLGAEILRLWTASTDYSGELSISDTILKRVVEGYRRIRNTLKFL
                     LGNLQDFALNDNAAPIGEMLEIDRYMLALMAHVQAETLDHFEHYEFQPAVSRLQIFCS
                     EDLGAFYLDILKDRLYTAGQNSNARRSAQTALYHITHSLLKLLAPILSFTAEEAWADL
                     HAEKDAVSIFTELFHVLPQPSDPAQIIEKWDRLRGLRADVMRKIEEVRATGAIGSSLA
                     AELDLYASGDQYQLLTSLEDDLRFVLIVSRASVHQAEGPLRIDVSASAQKKCERCWHH
                     RADVGIDPAHPEICGRCVDNLFGSGEPRHHA"
     gene            complement(3817..>4463)
                     /locus_tag="VIR04_09395"
     CDS             complement(3817..>4463)
                     /locus_tag="VIR04_09395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY9461004.1"
                     /translation="DMSAEAFIEQILVQGLNTRWLLVGEDFRYGHKRSGDVELLRNAG
                     RRYGFEVETIADVADEQGHRISSSEVRTALAVGNLKRAEHLLGHTYHISGHVIHGQKL
                     GRTLGYPTLNLNVPAHCAARSGIYVVRAHGLAPDAINGVASLGIRPTVSRSGLLLLEV
                     HLLDCTVQAYGKLARIEFLEYLRDEEKFPDFPTMIAAIANDAQSARDYFAFHGL"
BASE COUNT          918 a         1254 c         1321 g          970 t
ORIGIN      
        1 tcggcagcca actgcgccac attgcgttgg gtggccgggt gcagccacat ttcacggttc
       61 atggccgggg cgatcagcaa tggacagttt cctttggcca cgcacaaggc cgacaacaga
      121 tcgtcggcca gtccgtgcgc cagtctggcc atgaagtcgg tgctggccgg agcaatcagg
      181 atagcatcgg cgcccctggt cagattgata tgcgccatgc tgttgggcat gcgtgcatcc
      241 cacgcctcca ggaaaaccgg ccggccggat agggcttgca tggtggttgc cgtaatgaaa
      301 tgggtggccg cctcggtcat gaccacgtcg atctgcgcgc cgtgatcaag caatcgacgc
      361 agcaattcgg ccgacttgta gcaggcgatc ccgcctgtca ggcccagaat gatccgtttg
      421 ttcgccaaat cttgcatggt gaagacacct acttgtcgaa ggacgttgat tcggccgcct
      481 ggcggcgttc gcgccggtgc cggcgcaact cgtccagaat aagcaaaatt gcgccgcagg
      541 taatcgccac gtctgctacg ttgaaagcgg ggaagtacca agtattccaa taaaacagca
      601 agaaatcaat gacgtgtccg tgctgaattc gatcgactac gttgccgacc gcgccgccca
      661 gtatggccat aagcgaggcg caaaacaggg gttgatccaa actgcgccga agcagatgaa
      721 taatgagtcc cgcggcgatc aaggcgatgg cggtgaatag ccatcgctgc cagccctgtc
      781 cgtcggccag aaaactgaat gcggcgcccg ggttgtagag cagggtaaag tcaaaaaagg
      841 gcatcacgtg caggcgttcg ccataatgca ggtggccgtt gaaccatgct ttggccagtt
      901 ggtcggcgcc gacaatcaat accgccccca gtatccagat ccagaggcgg cccggccttt
      961 tcgggctgct tgagctgacg cttcctgctt caggcatggt gacgcggctc gccgctgcca
     1021 aacaggttgt cgacacaacg gccgcaaatt tcggggtgcg ccggatcaat gccaacatcg
     1081 gcccggtgat gccagcagcg ttcgcatttt ttttgcgcac ttgccgagac gtcgatgcgc
     1141 aacggccctt cggcctgatg cacgctagcg cgtgaaacga tcagcacgaa gcgcaagtcg
     1201 tcttccagac tggtcagcaa ctgatattgg tcgccgctgg cgtacagatc aagttcggcg
     1261 gcaagtgatg agccaatcgc gccggtcgcg cgcacttctt cgattttgcg catgacatcg
     1321 gcgcgcagac ctcgcagccg atcccacttt tcgataatct gtgccggatc agaaggctgc
     1381 ggcaacacat ggaaaagctc ggtgaaaatg ctgaccgcgt ctttttcggc gtgcaaatcg
     1441 gcccaggcct cctcggcggt gaatgacagt atcggggcca gcagcttcag caggctgtgt
     1501 gtaatgtgat agagggcagt ttgcgccgag cggcgcgcat tgctgttttg gccggctgta
     1561 tataagcggt cttttaaaat atccagataa aacgcgccga ggtcttcgga acaaaagatt
     1621 tgcagccggg atacggccgg ctggaattca tagtgttcaa aatgatccag cgtttcggcc
     1681 tgcacgtgcg ccatcaacgc cagcatgtag cggtcgattt cgagcatttc gccgatcggc
     1741 gcagcgttgt cgtttaacgc aaagtcttgc agattgccaa gcaggaattt cagtgtgttg
     1801 cgaatgcggc ggtagccttc aacgacacgc tttaaaatgg tgtcggaaat ggacaattcg
     1861 cccgagtagt cggtcgacgc ggtccacagc cgcagaattt cagcgcccag cgagtcggac
     1921 actttttgcg gagcaattac gttgcccacc gatttgctca tttttcggcc ttggccatca
     1981 acgacaaaac catgggtcag cagcgctttg tagggtggcg cgccgtaaag catgcagccg
     2041 gttagaaggg acgaatggaa ccagccgcga tgctggtccg acccttcaag gtacaggtcg
     2101 gcgggccaac cgagttcttc gccgcgcgat cccttgaatt cgtggttttt gcctcccagc
     2161 accgtggcgt gcgtcgtgcc ggaatcaaac cagacatcga gcgtatcgcg gtttttttcg
     2221 tactggtcgg cttcgtcgcc cagcagctct ctggggtcga gcgactgcca cgcttcgatg
     2281 ccgtgttgct cgatgcgctg agcaacctgc tcgatgagtt caggtgtgcg cggatgaagc
     2341 tggccggttt ctttatgcac gaaaaaggcc attgggacgc cccattgccg ttggcgcgat
     2401 agcgtccagt caggtctgtt ggcgatcatt gcgtgcaagc gtgcacgccc ccaggctgga
     2461 aagaattgcg tgttgtcgat gccctcgagc gcagttttgc gcagtgattt gcccgatttc
     2521 ggttgccggt ccatacctgc gaaccactgg cttgtcgcgc gataaatgat aggtgtttta
     2581 tggcgccagc aatgcatata gctgtgtgta tgctgttcca ccttaagcag ggaacccact
     2641 gaatccagcg ctttgacgat ttccgggttg gccttccaga tcatcagacc gccaaatacc
     2701 ggcagactgg cagcatagtg gccatcgccc atcaccggac tgatgatctg ttcatcggtc
     2761 agaccgttgg ccttgcatga aacgaaatcc tcgatgccat aggcgggcga tgagtgcacc
     2821 acgccggtgc cggcgtccag cgtgacataa tcacccagat aaactggcgc cttgcgccgg
     2881 tagccggggt ccagatcggc aagcggatgg atgaattcca gcccggccag cgccgaacct
     2941 ttggcggcgg cgatgatttc gcctctcaag ccccaacttt gcaggcatga ttcgaccagg
     3001 tcgcgcgcca gcagcaaaac cgatccgaca cccagcggtt cggtgagttt gaccaaaaca
     3061 taatcgatct cggggttgac gttcaaggcc tggttcgcag gcagcgtcca tggcgtcgta
     3121 gtccagatta ccgcggcgcc gtcgtcgacg tcagtcaagc cgaacgcgtg cgccagcttc
     3181 ttcttgtcgc tgaatttaaa tgctacatag accgccggat cggtgcggtc ggcgtattcg
     3241 acctccgcct cggccagggc cgagccgcaa tcaaaacacc agttgacggg tttcagtccg
     3301 cgaaaaacgt atcctttttc caaaatccgt cccagacaac gaagctcgtt ggcttcgttg
     3361 ctgaagttca tggtcagata agggtggtcc cagtcggcca ggacgcccag acgtttgaag
     3421 tctttacgct gacgatcaat ttgttcgagc gcataggccc gcgctctgga ttgcacttgg
     3481 tcgaccggta aattcttgcc gtatttcttt tcaatctgga tttcaatcgg catgccgtgg
     3541 caatcccagc ccggcacgta gggcgcgtca aagcctgcca tggagcggct tttcaggata
     3601 atgtctttca gtattttgtt aaccgcgtgg ccgatgtgaa tatcgccatt ggcgtacgga
     3661 gggccgtcgt gaagaatgaa ttttgggcgt ccggcgctgg cggcccgaat ggccgcgtag
     3721 accttctttt cttcccactg tgcgatccag cccggctcgc gcttgggcag gttacctcgc
     3781 atgggaaaag gtgtttcggg caaatttaaa gtgtttctat agtccatgga aggcgaaata
     3841 gtcgcgagcg ctttgcgcat cgttggcgat ggcggcgatc atagtgggga aatcgggaaa
     3901 tttttcttcg tcccttaggt actcaaggaa ctcaatgcgt gccagtttac cgtatgcctg
     3961 aacagtacag tccagcagat gcacctcgag caagagcaaa ccgctgcgcg agacggtagg
     4021 gcgaatgccc aggctggcca cgccattgat ggcgtcgggt gccagtccgt gggcgcgtac
     4081 gacatagatg ccggaacggg cggcgcagtg agccggcacg ttcagattca gggttgggta
     4141 gcccagcgtg cggcccagct tttggccatg aatcacatgg ccgctaatgt ggtaggtgtg
     4201 gcccagcaaa tgttcggccc gcttcaggtt gccgaccgcc agtgcggttc gcacctcgga
     4261 gcttgaaatt cgatgccctt gttcgtcggc cacgtcggcg atggtttcga cttcgaagcc
     4321 atatcgccgg ccggcgtttc gcagcagttc gacatcgccg ctgcgtttat ggccatagcg
     4381 gaagtcctcg cccacgagca gccagcgggt attgagcccc tgcaccagaa tttgctcgat
     4441 aaaggcttcc gccgacatat ccg
//
LOCUS       DATCXU010000417         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_88467,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000417 DATCXU010000000
VERSION     DATCXU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88467"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /gene="glnE"
                     /locus_tag="VIR04_09400"
     CDS             <1..1695
                     /gene="glnE"
                     /locus_tag="VIR04_09400"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY9461005.1"
                     /translation="EAAYCFLRRTEHMLQYREDEQTHLLPRDPVLYNGLAHAMGLEPP
                     IFKATLEAHRSFVTHAFRDAFRTAGIGGENEAQGKTASVASVNANMNLDDQIKVRLGE
                     QAAMISQRADALLDSHRIQGLPRTSRRRVETLLPAAIDAASRSDNPPQTAIRLLDLIE
                     QIAQRSAYLALLAEYPETLARVARIVSSSPWASQYLGRYPLLLDSLIEWRSLLEPPNM
                     AQIAEQLHNDLDACLLPDGQPDIEQQMNLMRDVQHQVTFQLLAQDLEGVLSVEKLADQ
                     LSALADMLLAETIVRVWPLVQTKGQPRTNLAPPKFAAIAYGKLGGKELGYASDLDLVF
                     IYDDPRDDAVEIYARLGRRISSWLSTMTSSGRLYEIDLRLRPDGEAGLLAVSLDAFAQ
                     YQTKHAWVWEHQAITRARFAAGDAAIGARFEEIRHRVLLLPRDPDVLKEQVRDMRRKI
                     SAGHPNRSKDFDLKHDRGGMVDVEFATQYLVLCHAARYPILLKNLGNIALLRLSAEAG
                     LIPAGLSADAANAYREFRKLQHALRLQGAEKARVPPDSVSAGRTAVSKLWNTVIGD"
     gene            1741..2391
                     /locus_tag="VIR04_09405"
     CDS             1741..2391
                     /locus_tag="VIR04_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEY9461006.1"
                     /translation="MSDTERPVLVLPDGRKKVLLHSCCAPCSGEVMEAIAASGIDYSI
                     FFYNPNIHPLREYEIRKNENIRFAEKHHIEFIDADYDRDDWFKRIKGFENEPERGRRC
                     TLCFDMRFERTALYAHEHGFDTITSSLGISRWKDMDQINGSGERAAARYPELIYWTYN
                     WRKGGGSARMIEISKREEFYQQEYCGCVFSLRDTNRHRRAQNREPVKIGVKYYGKQ"
     gene            complement(2427..>4371)
                     /locus_tag="VIR04_09410"
     CDS             complement(2427..>4371)
                     /locus_tag="VIR04_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY9461007.1"
                     /translation="GLAQRIVNDEVPENLKGKRVLALDMAALLAGAKYRGEFEERLKA
                     VLKELSQDDGKTIVFMDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEY
                     RKYIEKDAALERRFQKVLVNEPDVESTIAILRGLQERYELHHGVAITDPAIVAAAELS
                     NRYITDRFLPDKAIDLIDEAAARIRMEIDSKPEVMDKLDRRIIQLKIEREAVKKETDE
                     SSLRRLKAIEDELLALQREYNDYEEIWKAEKAAVQGSQSIKEEIERTRAEMAELQRKG
                     QFDKLAELQYGKLPELEGRLKAAEAGQQAAPDSDHPRLLRTQVGAEEIAEVVARATGI
                     PVAKMMQGERAKLMGIEDILRQRVVGQDDAIALVADAIRRSRAGLSDPSRPYGSFLFL
                     GPTGVGKTELTKALASFLFDSEDHLIRVDMSEFMEKHSVARLIGAPPGYVGYEEGGHL
                     TEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNL
                     GSQHIQGMAGQPYDVIKEVIWDELKTAFRPEFLNRIDEVVVFHSLDSKNIEAIARIQL
                     QRLGQRMALQEMRLEVSDAALAQIARAGFDPVFGARPLKRAIQQYIENPIAKLILEGK
                     FGPKDVVPVDWQNDRFVFERTLQ"
BASE COUNT          920 a         1355 c         1160 g          936 t
ORIGIN      
        1 gaggccgcct attgcttttt gcgacgcacg gaacatatgc tgcaataccg ggaagacgag
       61 caaacccatc tgcttccacg cgatcctgtc ctgtacaatg gattggctca cgccatggga
      121 cttgagccgc ccatattcaa agccactctt gaagcgcacc gatccttcgt aacgcacgca
      181 tttcgcgacg ctttccgcac cgccggaata ggcggcgaga acgaggcgca aggcaagacg
      241 gcttctgtcg caagcgttaa cgccaacatg aacctcgacg accagattaa agttcgtttg
      301 ggcgagcagg ccgccatgat ctctcagcgc gccgacgccc tgctggacag ccatcgcatc
      361 cagggtctgc cgcgaaccag ccgacgccgg gtggaaacgc tgttgccggc cgccatcgat
      421 gccgcatccc gcagcgataa tccgccgcaa acggccattc gattactcga tcttattgaa
      481 caaatagcgc aacgcagcgc ctatctggcg cttttggccg aatatcccga gacgctggcg
      541 cgcgtcgcgc gtatcgtcag ttccagccca tgggcctcgc aatatttggg tcgatacccg
      601 ctgttgcttg acagcctgat cgaatggcgc tctttgctgg agccacccaa catggcccag
      661 atagcggaac aattgcacaa cgacctcgac gcatgcctct tgcctgacgg acagcccgac
      721 atcgagcagc agatgaactt aatgcgcgac gttcagcacc aggtcacctt ccagcttctc
      781 gcccaggacc tggaaggcgt tctaagcgtc gaaaaactgg ctgaccagct ttcagccctg
      841 gccgatatgt tattggcgga gacgatcgtg cgcgtctggc cgctggtgca gaccaaaggg
      901 cagccccgaa ccaatctggc gccccccaaa ttcgccgcca tcgcctacgg caagctaggc
      961 ggcaaagagc ttggctacgc gtcggacctt gacctggtat tcatttatga cgacccgcgc
     1021 gacgacgccg tggaaatcta tgccaggctc gggcggcgga tatcatcgtg gctgtcgacc
     1081 atgacctctt cggggcgctt gtacgaaatt gacctgcgtt tgcggcctga tggcgaagcc
     1141 gggttgttgg cggtttcgct ggacgccttt gctcaatacc agaccaaaca cgcctgggtt
     1201 tgggaacacc aggccatcac ccgggcgcgt tttgccgctg gcgatgctgc aatcggcgcc
     1261 cgcttcgagg aaatccggca cagagtattg ctcttgcccc gtgatccgga tgtgctgaaa
     1321 gaacaggtgc gcgacatgcg ccggaagatc agcgcaggcc accctaatcg atcgaaagac
     1381 ttcgatctga aacatgaccg cggcggtatg gtcgacgttg aattcgcgac gcaatatttg
     1441 gttctgtgtc acgccgcccg gtacccgatc ctgctaaaaa acctcggcaa catcgccctg
     1501 ttgcgactgt ccgccgaagc cggcctgata cccgccggac tgagtgccga tgccgccaac
     1561 gcttaccgcg aattccgcaa gttgcagcac gcgctgcgct tgcagggagc cgaaaaggcc
     1621 cgcgtgccgc ctgattcggt cagcgcgggg cgcaccgccg tgagcaagct ttggaatacc
     1681 gtgattggag attaaaatga gcagcatctt ccgttcgcac ttatcgtagt cccgcttatc
     1741 atgtctgata ctgaacgccc tgtacttgtc ctgcccgatg gccgtaaaaa ggttcttttg
     1801 cactcttgct gcgcgccctg ctcgggcgag gtcatggagg ccatagccgc atcgggtatc
     1861 gattactcca tcttttttta taacccgaac atccacccct tgcgggaata cgagatccgc
     1921 aagaacgaaa acatccgatt tgccgaaaaa catcacatcg aattcattga tgccgactat
     1981 gatcgcgacg actggttcaa gcgcatcaaa ggctttgaaa acgaacccga acgcggcagg
     2041 cgctgcaccc tatgctttga catgcgcttc gaacgcactg ccttgtatgc ccacgaacac
     2101 gggttcgaca ccattaccag ttcattgggc atttcgcgct ggaaagatat ggaccaaatc
     2161 aacggcagcg gcgagcgcgc ggccgcgcgc taccccgaac tgatctactg gacctacaat
     2221 tggcgcaaag gcggtggttc tgcgcgcatg atcgaaatca gcaagcgcga ggagttctac
     2281 cagcaagaat actgcggttg cgttttttcg ctgcgcgaca ccaaccgcca tcgccgggct
     2341 caaaaccggg agcccgtcaa aatcggcgtc aagtactacg gcaaacagta gcgccggccg
     2401 ggccaccggg cgcggcgcca atctgtttat tgcagcgtgc gctcaaacac gaaccgatca
     2461 ttttgccaat cgaccggcac gacatccttg ggcccgaatt tgccttccag aatcagtttg
     2521 gcgatcggat tttcgatgta ctgctgaatt gcgcgcttaa gcggtcgagc gccgaaaaca
     2581 gggtcgaaac cggcacgcgc gatctgcgcc aacgccgcgt cggatacctc gagccgcatt
     2641 tcctgcaatg ccatccgctg ccccagacgc tgcaattgaa tccgggcgat ggcttctata
     2701 ttcttcgaat ccaggctgtg gaatacgacc acctcgtcaa tccggtttaa aaactcgggg
     2761 cgaaatgcgg ttttcagttc atcccagata acttctttaa tcacatcgta aggctgtccg
     2821 gccataccct gaatatgctg cgaccccaaa ttcgatgtca tgataatgac cgtattgcga
     2881 aaatcgactg tgcggccctg cccgtcagtc aggcggccgt cgtcgagcac ttgcaacaac
     2941 acattgaaca catcggggtg ggccttttca atttcgtcga gcaaaatgac gctatatggt
     3001 ttgcggcgca cagcttcagt caagtgacca ccctcttcgt agcccacata cccgggcggc
     3061 gcgccaatca agcgcgcaac cgagtgcttc tccatgaatt cgctcatgtc gacgcgaata
     3121 agatggtcct cggaatcaaa tagaaagctg gccaaagcct tggtaagctc cgttttgccc
     3181 accccggtag gccccagaaa caggaacgag ccataaggac gagaagggtc tgacagaccc
     3241 gcgcgcgaac gacgaatggc atcggccacc agcgcgatgg catcgtcctg cccgactacg
     3301 cgctgacgca ggatatcttc gatgcccata agcttggcac gctcgccctg catcattttc
     3361 gccacgggaa tccccgtggc ccgcgcgaca acctcggcaa tttcctcggc cccgacctgc
     3421 gtgcgcaaca ggcgcggatg atcggagtcg ggcgccgcct gctgccctgc ttcggccgct
     3481 ttcagccgcc cttcaagttc cggcaatttg ccatattgaa gctcggccag cttgtcgaac
     3541 tgtcccttgc gttgcaattc agccatttcg gcccgggtgc gctcgatttc ttccttgatc
     3601 gattgcgagc cctgaaccgc cgctttttcg gccttccaga tttcttcata atcgttgtat
     3661 tcgcgttgca acgccaacag ttcgtcttcg attgccttca atcggcgcag cgagctttca
     3721 tcggtttctt tcttgacggc ttcgcgttca atcttgagct gaataatgcg acggtcaagc
     3781 ttatccatga cttcgggctt ggagtctatt tccatgcgta tgcgcgccgc tgcttcgtcg
     3841 atcagatcaa tcgccttatc cggcaagaat cggtcggtga tgtagcgatt ggagagttca
     3901 gcggccgcta cgatcgccgg atcggtgatg gccacgccat gatgcaattc gtaacgctct
     3961 tgcaagccgc gcaaaatggc tatcgtggac tcgacatcgg gctcgtttac cagcaccttt
     4021 tgaaaacgtc gctcgagcgc ggcgtctttt tcgatgtact tgcgatattc gtccagcgtc
     4081 gtcgcgccaa cgcaatgcag ttcgccgcgc gccagagcgg gctttagcat attgcccgcg
     4141 tccatcgcgc cttcggcctt gcccgcgccc accattgtgt gcaactcatc cataaacaca
     4201 atggttttgc catcgtcttg cgatagttct ttcaggaccg ccttcaggcg ttcctcgaac
     4261 tcgccccgat atttggcgcc cgccagcaac gccgccatat ccagcgccag aacgcgcttt
     4321 cccttcaagt tttcggggac ttcgtcgttg acgatacgct gcgccagacc c
//
LOCUS       DATCXU010000418         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_280534,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000418 DATCXU010000000
VERSION     DATCXU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280534"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VIR04_09415"
     CDS             complement(<1..710)
                     /locus_tag="VIR04_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY9461008.1"
                     /translation="MKVLITDYDFPDIALEQALCDAAGINLAVAQCRTESDVIAASQG
                     CQGLLAQYAPINAQVFTARPEIRVVSRYGAGFDTVNIDDAKKHGVWVANSPDYGIGEI
                     ATHALAMMLALLRHIVAYDRDVKAGTWDFNSTGPLQRVSDMTVGIVGLGRIGKRMAHV
                     CRNCFNRVIACDPYIIDGDFPAYVERVDFKQLFSQADAISLHVPLNDETRGMVNRTVL
                     GWMKPGSVLVNTARGPIV"
     gene            complement(740..2467)
                     /locus_tag="VIR04_09420"
     CDS             complement(740..2467)
                     /locus_tag="VIR04_09420"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY9461009.1"
                     /translation="MDDKKGPARRSQAWFGRQDRDGFIYRSWLKNRGIPHDQFDGRPV
                     IGICNTFSELTPCNSHFRTLAEQVKIGVWESGGFPLEFPVMSMGETLLRPTAMLFRNL
                     ASMDVEESIRGNPLDGVVLLMGCDKTTPSLLMGAASCDLPTIGVSGGPMLNGKFCGRE
                     LGSGTDVWRMSEEVRAGQMSQEQFFQAESCMSRSHGHCNTMGTASTMASMVEALGMSL
                     PGNAAIPAVDARRNVLARESGRRIVGMVREDLYMSKILTRAAFENAIRVNAAIGGSTN
                     AVIHLLAIAGRIGIKLSLDDWDVFGHALPCLVNLQPSGHYLMEDFYYAGGLPAVIREL
                     ESVLDHNTLTANGKTLWENSKDAPNWNRDVIHTYTEPFKADAGIAVLRGNLCPDGAVI
                     KPSAATPALLKHTGRAVVFENSDDMHKRLNDETLDVDENCVLVLKNCGPKGYPGMAEA
                     GNMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVAPEAAAGGPLALVQEGDIIEL
                     DVPARKLHLHVSDDELARRRATWQAPPAPMSRGWVKLYVEHVQQANLGADLDFLVGKS
                     GSAVPKDNH"
     gene            2659..3417
                     /locus_tag="VIR04_09425"
     CDS             2659..3417
                     /locus_tag="VIR04_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9461010.1"
                     /translation="MATPVHYDFSSQHAVVTGGASGIGLAIAQRLSGDGATVSLWDRN
                     QDALEHAKSLFGAKPPQIIAVDVTSESAIATAYATTLKAAGAVDILVTSAGITGPNTT
                     VSDYAVADWDNVMNINLRGPFLCCRAVVPQMRNKGYGRIVNIASIAGKEGNPNASAYS
                     ASKAGVIALTKSLGKELAQSGICVNCVTPAAVRTGMFAQMTQTHIDYMLSKIPMGRFG
                     TPEEIAAVVTWLCSAECSFSTGAVFDVSGGRATY"
     gene            3465..>3779
                     /locus_tag="VIR04_09430"
     CDS             3465..>3779
                     /locus_tag="VIR04_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9461011.1"
                     /translation="MPIKIIEPQRLYRQISSQLRTLILAGEFPVGSRLPAERHLSAQL
                     GVSRPSLREALIALEVQGYIEVRMGSGIYVCDPPLAQQEFDLSAEEGPLELINARALL
                     EGE"
BASE COUNT          827 a         1119 c         1058 g          775 t
ORIGIN      
        1 tccacgatcg gcccgcgcgc cgtattgacc aggacgctgc ccggcttcat ccagcccagc
       61 acggtgcggt tgaccatgcc gcgggtttca tcgttaagcg gcacatgaag agatatggca
      121 tcagcttggg agaataattg cttgaaatca acccgctcaa cgtaggcggg gaaatcgcca
      181 tcaataatat aggggtcaca ggcaataacc cgattgaaac aattgcggca tacgtgggcc
      241 atgcgcttgc caatgcggcc caaaccgaca attcccacgg tcatgtccga tacgcgctgc
      301 aggggcccag tggaattgaa atcccaggtt ccggctttca cgtcgcgatc ataggcaaca
      361 atatggcgca gcagcgcgag catcatcgcc aacgcgtgag tcgcaatttc accaatgcca
      421 taatcaggcg aattggccac ccataccccg tgtttctttg cgtcgtcgat attgacggtg
      481 tcaaagcccg ccccgtagcg gctgacgacc ctgatctccg gacgggccgt gaatacctgg
      541 gcattgatgg gcgcatattg ggccagcagc ccctgacacc cttgcgatgc ggcgatgaca
      601 tcggactcgg tgcggcactg ggcgaccgcc aggttgattc ccgccgcatc gcacagggcc
      661 tgctccagcg caatgtcggg gaaatcgtaa tcagtaatta gaactttcat ggcattgcac
      721 ttcggcggat gtggcgcgat cagtgattat ccttgggcac ggcggagccg cttttgccaa
      781 ccaggaaatc cagatcggcg cccagattgg cctgctgcac atgctcgaca tacagtttga
      841 cccaaccgcg cgacatcgga gcaggcggcg cctgccacgt ggcgcgccgt ctggcaagct
      901 cgtcatcgga cacgtgcagg tgcaatttgc gcgccggcac atccagttcg attatgtcgc
      961 cttcctgaac cagggccagc ggcccgcccg ccgccgcttc gggcgcgaca tgcaatacca
     1021 ctgtgccgta ggctgtgcca ctcatgcgcg cgtcggacac gcgcaccatg tcggtaatgc
     1081 ccttgcgcaa tactttcggc ggcaacggca tattgcccgc ctcggccatc cccgggtatc
     1141 ccttgggccc gcaatttttt aacaccagta cgcaattttc atcgacatca agcgtttcgt
     1201 cattaaggcg cttatgcatg tcatcgctgt tttcgaacac caccgcgcga ccggtatgtt
     1261 tgagcaaggc cggggtcgcg gcggacggct tgatgaccgc gccgtccggg cataagttgc
     1321 cgcgcaacac cgcaatgccc gcatcggcct tgaatggttc ggtgtaagta tgaatgacat
     1381 cgcgattcca gttcggcgca tctttgctgt tttcccacag ggtcttgcca ttggccgtca
     1441 atgtattgtg atccagcacc gattccagtt cgcgaataac cgccggcaaa cccccggcgt
     1501 agtaaaagtc ttccatcagg taatggccgg agggctgcag gttgaccagg caaggcaatg
     1561 cgtgaccgaa cacatcccaa tcgtccagag acagcttgat gccgatgcgc ccggcgatgg
     1621 ccaataaatg aatgaccgcg ttggtcgaac cgccaatcgc ggcgttcact cgtatcgcgt
     1681 tttcaaacgc cgcgcgggtt agaatttttg acatgtaaag atcttctcta accatgccaa
     1741 cgatgcgccg gcccgattca cgcgccagga cattgcggcg tgcatcgaca gcgggaatgg
     1801 cagcgttgcc cggcagactc atgccaagcg cttcgaccat gctggccatg gtggaggccg
     1861 tgcccatcgt attgcagtga ccgtgcgagc ggctcatgca gctttcagcc tggaagaatt
     1921 gctcttgcga catttgcccc gcgcgcacct cctcggacat cctccagacg tctgtgccgg
     1981 agcccagctc gcggccgcaa aacttgccgt tcaacatggg gccgccagac acgcctatag
     2041 ttggcaaatc gcacgatgcc gcgcccatca gcagcgacgg cgtagttttg tcgcagccca
     2101 tcagcaacac aacgccgtca aggggattgc cgcggatgga ttcctcgacg tccatgctgg
     2161 cgagattgcg aaacaacatt gcagttggac gcaataaggt ctcgcccatc gacatcacgg
     2221 gaaattccag cggaaaaccg cccgactccc acacgccgat cttgacctgt tcagccagag
     2281 tgcggaaatg ggaattgcaa ggcgtcagtt cggaaaaggt attgcaaata ccaataaccg
     2341 gccggccatc gaactgatca tgcggtatgc cgcgattctt cagccacgag cgataaatga
     2401 aaccatcgcg atcctggcgg ccaaaccaag cctggctgcg ccgcgcaggc cctttcttgt
     2461 catccaactt tgtctcctgt gccgatgaac ccccggcctt tcattttgaa cggtggccaa
     2521 cccatgcgtc cgtatggcag tctagtacag aactttgcaa gggtaaagtg gtaataccaa
     2581 atgccaaaat cactctgttc gaatcgatga atacagtgta tgtttaagcc cattgaaagg
     2641 cagcgcaagg agaaagccgt ggccacacct gttcactatg atttttcatc tcaacacgcg
     2701 gtcgtcactg gtggcgcgtc gggtatcggc ctggccatcg cccaacgact gtcaggcgat
     2761 ggcgccacag tcagtttgtg ggatcgcaac caggacgctc tggaacatgc aaaatctttg
     2821 ttcggcgcaa agccgccgca gatcatagcg gtggacgtca ccagtgaaag cgcgatcgcc
     2881 accgcctatg ccaccacgct caaggccgcg ggagcggtgg atattctggt gaccagcgcg
     2941 ggcattactg gccccaacac caccgtttca gattatgcgg tggcggactg ggacaatgtc
     3001 atgaatataa atctgcgcgg cccctttttg tgctgccgcg cggtagtgcc gcaaatgcgc
     3061 aataagggct acggccggat cgtgaatatt gcgtcaatag cgggtaaaga aggcaacccg
     3121 aacgcatcgg cctacagcgc gtccaaagcg ggggtgattg cgcttaccaa atcgctggga
     3181 aaagagctgg cgcaatcggg aatctgcgtt aattgcgtga cgccggccgc tgtgcgcacc
     3241 ggcatgttcg cgcaaatgac gcagacgcat atcgattaca tgctgtccaa aatccccatg
     3301 ggccggtttg gcacgcctga agaaattgcg gcagttgtaa cgtggctttg ctcggcggaa
     3361 tgttcttttt ccaccggcgc agtgttcgac gtatcgggcg gacgcgccac ttattgacgc
     3421 cacccactaa aataggcgat aaataaaatg gggatccgcc tttcatgcct atcaaaataa
     3481 tcgagccgca acggctttat cgtcagatta gcagccagtt gcgcacactg attttggcgg
     3541 gcgagtttcc ggtcggctcg cgattgcccg ccgaacggca tttatccgcg caacttggcg
     3601 taagccggcc ttctctgcgc gaagcgctta ttgcgctgga ggttcagggt tatattgaag
     3661 tgcggatggg gtcaggcatt tacgtgtgcg acccgcccct cgcgcaacaa gagttcgacc
     3721 tttccgccga ggaagggccg ctggaactaa tcaacgcccg ggcactactc gaaggcgaa
//
LOCUS       DATCXU010000419         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_459227,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000419 DATCXU010000000
VERSION     DATCXU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459227"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VIR04_09435"
     CDS             <1..293
                     /locus_tag="VIR04_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY9461012.1"
                     /translation="AAIVASAFIELGSLHPDPSAGEMWFRQGVATLEALCRDEFTGGS
                     SPRGLLKNSCYSMPHREGINGATMFGDFYFIEALCRIVLPGRFRPAHPRITF"
     gene            327..1244
                     /locus_tag="VIR04_09440"
     CDS             327..1244
                     /locus_tag="VIR04_09440"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY9461013.1"
                     /translation="MNELKGICSAICTPMDDNGAVSVARLKNHIDKMIEAGVHIIAVC
                     GGTGEFAFLDCEEKRQIAEVSARHINGRAKLIVQASAIRTEDAVEAALHAQGVGADAV
                     LVLPPYFEGPVPDGVYYHYARISEKIEIPIMAYNVPVHSGFDITPEFFKCLTEIDNVK
                     YIKDTSGDFVRIQELLASGATVFNGSDPFAFYALLAGCNGCFWGAVNAMPEEAVQLYK
                     LVQTGKLTEAVTLWKAMLPANLFFWSHAYNPSIKAATNIMTGPVGMCRKPVMPLAPIE
                     EQALRAALQSLVDLRNGASSIRQAGALTE"
     gene            complement(1251..1673)
                     /locus_tag="VIR04_09445"
     CDS             complement(1251..1673)
                     /locus_tag="VIR04_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY9461014.1"
                     /translation="MAATPLFHLAFPVNDLAEARKFYGELLGCPEGRSSDRWVDFNFY
                     GHQIVAHLSPSETGQANTSDVDGHQVPARHFGAILSLPEWEALAAKLKAANIKFIIEP
                     YIRFKGQVGEQATMFFLDPSGNALEFKSFADMNYIFAK"
     gene            complement(1853..>3016)
                     /locus_tag="VIR04_09450"
     CDS             complement(1853..>3016)
                     /locus_tag="VIR04_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY9461015.1"
                     /translation="GLGAIISPPVMGAAAFIIAEFLKISYLDVLLMAIIPTGLFYLAL
                     FLMVEIDVRKYGMGQTVIKKVDTVWNLTKHYWFHFLSLISIVAFMMWGFSPVLSVFWA
                     TVVAFLTSFLRPDSAMISYDLFRGKGSIKEHLVQSKLVQALRAGSVGMLSVGATCAGA
                     GIIVGVVTLTGLGLKFSTIVIDYAGGSLLLTAVYTALVVWIIGLAVPVTASYIICAVI
                     TAPALIKLGVPDFAAHMFIFYYAVLSEVSPPTALSPFAASAITGGDPYKTTMQCWKYT
                     IPAFLVPFIFVLDPSGQSLLLMGSTKALATADWWMIARVTVTAAAGIFAIAVGFQGWL
                     RVKATMIERIMLIVAGFALVYPGPISNAIGFALVFIALAVHWRRGSSMKLAGS"
BASE COUNT          795 a          851 c          779 g          591 t
ORIGIN      
        1 ccgcagccat cgtcgcatcg gcattcatcg aactgggcag cctgcatccc gaccccagtg
       61 ccggcgaaat gtggttcaga caaggtgtcg caacccttga agccctgtgc cgcgatgaat
      121 ttacgggcgg atcttcgcca cgcggcttgc tcaaaaatag ttgctactcc atgccacacc
      181 gggaagggat caacggcgcc accatgttcg gcgatttcta tttcatcgaa gcgctgtgcc
      241 ggattgtgct acccggccgc ttcaggcccg cccatccgcg cattaccttc taattcactc
      301 aaacctgaac tacaaggata cgacccatga acgagctcaa aggcatatgt agcgcaattt
      361 gcactcctat ggatgacaac ggcgccgtca gcgtcgcccg gctgaaaaac cacatcgaca
      421 aaatgatcga ggccggcgtg cacattattg cagtgtgcgg aggaaccggg gagttcgcat
      481 tcctggattg cgaagaaaag cgtcaaatcg cagaggtctc ggccaggcac atcaacgggc
      541 gcgccaagct gatcgtacaa gcttcggcca tccgcacaga agacgccgtc gaggcggcgc
      601 ttcatgccca gggagtgggc gcggatgcgg tgctggttct gccaccatac ttcgagggac
      661 cggtacctga tggcgtttac tatcactacg ccagaatttc agaaaaaatc gaaattccga
      721 taatggccta caacgtaccc gtgcattccg gatttgacat tacccctgaa ttcttcaaat
      781 gtttgacgga aatcgacaac gtcaaataca ttaaagacac cagcggcgat tttgtccgga
      841 tacaagagct gctggctagt ggagcaaccg ttttcaatgg ttcagaccct ttcgcttttt
      901 atgccttgct ggccgggtgc aacggatgtt tctggggcgc ggtaaacgcc atgcctgaag
      961 aagccgtgca actctacaag ctggtgcaaa cagggaaatt aactgaagca gtcactcttt
     1021 ggaaagcaat gttgcctgcc aacttgttct tctggagcca tgcatacaac ccctcgataa
     1081 aagcagccac caacatcatg accggccccg tgggaatgtg caggaaacct gtcatgccat
     1141 tggcgcctat tgaagaacag gcgctcaggg cagcattgca aagcctcgtc gacctgcgca
     1201 atggcgcgtc ctcaatccgt caggcagggg ctctgactga gtaaatccgg ctacttcgcg
     1261 aaaatgtagt tcatgtcggc gaacgatttg aattccagag cattgcccga cggatcaaga
     1321 aagaacatgg tcgcctgctc gccaacctgg cccttgaaac ggatatacgg ctcgataatg
     1381 aatttgatgt tcgccgcttt gagctttgcg gccagcgctt cccattcggg caaagacaga
     1441 atggcgccaa aatgacgcgc gggaacctga tgtccgtcca cgtcgctggt attggcctgc
     1501 ccggtttcag acggagataa atgggccacg atctgatggc cgtaaaaatt gaaatcgacc
     1561 cacctgtctg aactgcgtcc ttcgggacag cccagcagtt ctccgtaaaa cttgcgcgcc
     1621 tcggccagat cgttaacagg gaaagccaag tggaataatg gtgtggcagc catacaggtt
     1681 ccttcaataa gcgaattcag ataaagtcac attcgaatga caatgcaaac ggcattcagc
     1741 ctaatcatag tgctgaatgc cgatattatc ctgcgaaccg atgatcgccg tcaggacggc
     1801 gaagattcag gcaatcaatg gccgatgcca atgtgttggc gccggccacc agtcatgatc
     1861 cagccaattt catcgaagaa ccccgccgcc aatgcaccgc cagggcaatg aacaccaaag
     1921 cgaaaccgat tgcatttgat atcggcccag gatagaccag agcgaaaccg gccacaatca
     1981 gcattattct ttcgatcatg gtggccttca cccgcaacca accctggaag cccaccgcaa
     2041 tggcaaaaat gccagccgcc gccgtaacgg tgacacgggc gatcatccac caatccgctg
     2101 tggcaagcgc cttggtcgag cccatcagca acaggctctg gccgcttggg tccaatacaa
     2161 aaataaaggg gaccaggaat gccggtatgg tgtatttcca acactgcatg gtggtcttat
     2221 acgggtcgcc tcctgtaatc gccgaagcgg caaagggtga caacgctgtc ggtggcgaga
     2281 cttcggacag aaccgcatag taaaagatga acatatgggc cgcgaaatcg ggtacaccca
     2341 gcttgatcag ggccggcgcg gtgatgaccg cgcagataat ataagacgcc gtgacgggca
     2401 ccgcgagacc tatgatccag accaccagcg cggtgtagac ggctgtaagc agcagcgagc
     2461 cgcccgcata atcaatcacg atggtgctga atttaagccc caggccggtc agagtcacta
     2521 caccgacaat aatgcccgcg cccgcgcaag tggcgcccac gctaagcatg ccaaccgagc
     2581 cggcccttaa tgcctgaaca agcttggact gaaccagatg ttctttgatg gagcctttgc
     2641 cacggaacaa atcatacgaa atcattgccg aatcggggcg caggaaactg gtcaaaaatg
     2701 ccacgaccgt cgcccagaac accgacaaca cgggtgaaaa accccacatc atgaacgcca
     2761 caatagaaat cagcgacaag aaatggaacc agtaatgctt cgtcagattc catacggtgt
     2821 cgaccttctt gatgaccgtc tgacccattc cgtatttgcg gacgtcaatc tcgaccatta
     2881 aaaataatgc aagataaaac aggcccgtcg ggattattgc catcagcaat acgtcaagat
     2941 acgaaatctt tagaaactcg gcaatgatga acgcggcggc gcccatcacc gggggcgaga
     3001 taatagcgcc cagccc
//
LOCUS       DATCXU010000420         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_830961,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000420 DATCXU010000000
VERSION     DATCXU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830961"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1513)
                     /locus_tag="VIR04_09455"
     CDS             complement(290..1513)
                     /locus_tag="VIR04_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9461016.1"
                     /translation="MSIRNIGLLPQVSLAHFASHFHIMALPALLPILPQFFGVGFIEL
                     GLALSVFNIVSALVQAPLGFAVDHYGAKRVLVSGLVLGSSCFVAVALIPNYACLLIAM
                     GLAGVANGVYHPADYSLLSKGINPSRIGRAFSIHTFTGFLGSAIAPAFLLGIAALSNP
                     RIAFAVTAVIGFVIAALILIPGSGISKVPQTRVVANSGKPLTKSRRPPIFTPMIGVLT
                     LLFALLSLSTGAIEKFSVAAFVQGHEVTLSWANTALTAFLFASAFGVLFGGALADRTK
                     RHGLVAAVAFGLAAVFTAIVAMTSLNDLALTITLGTIGFLTGVITPSRDMLVRAAAPA
                     GSEGKAFGIVTSGFNVGGATGPMIFGWMLDNGHPNSIFWASVIFMSLTVVLTLLQEWH
                     MAKARPNSSRLAQAK"
     gene            1719..2507
                     /locus_tag="VIR04_09460"
     CDS             1719..2507
                     /locus_tag="VIR04_09460"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9461017.1"
                     /translation="MPQAKNLSNLNGKVALVTGSAIGIGRAIAIRLAQDGADLALFDI
                     NQEETQNTAAMVRAIGRRCEVVRGDVGDYVQVQRAVTHFIASLGAIDVAVHNAGITCV
                     GTVAETSIEDFHKVFRVNVDGIFYGCKAVVPHMMERGSGKIINTSSWLGKAGKPNYGA
                     YSASKAAVIGLTQSLAMEVARYQINVNAVCPGVIIETGMRDEADAASIRQGLPTAKQR
                     ETEIPIGRVGLPNDVARVVSFLSSDQSDYMTGQAVNVTGGLWMQ"
     gene            2527..3312
                     /locus_tag="VIR04_09465"
     CDS             2527..3312
                     /locus_tag="VIR04_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY9461018.1"
                     /translation="MTRNQILCDVNDGVATVTLNNLPLNVVTLEMSRELSKVLPALAA
                     DSAVRALVLTGAGERAFCAGSDISEFPNMLGSGKIVPMKLAFENQVYGTVAAFPKPTI
                     AAISGLAYGGGLELAVCCDFIVIEEHTRLCLPEIKLGVFPGSGGTVRVTRRIGASRTK
                     EMMFFGEPIDAPTALLWGLVNRVVPAGQSRHTARAMAQDLARQPNQALQLCKAAIALS
                     CDTPEDEAIRDTMDLSDKVFATEDCKEGVRAFFAKEKPHFKHR"
     regulatory      3459..3557
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3603..4385
                     /locus_tag="VIR04_09470"
     CDS             3603..4385
                     /locus_tag="VIR04_09470"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEY9461019.1"
                     /translation="MPGSQQPLSLNEQVVLVTGGARGLGAHIVRAFQREGARVVVNYF
                     RSAGSAAALAAQRPDRTLAIMADVRDDGAVKAMVNQAQQHFGAQVTTVVNNALPDFQF
                     NGDQRHQADALTWEDIHHQMEGTVRGALNTIQAALPGMRKLGFGRVINVGTNLFQNPV
                     VPYHDYTAAKAALLAFTRTLSHDLGPEGITVNMVSGGLLRTTDASAATPEAVFEFIAA
                     TTPLRRVTTPAEFADALLFFASPWSRSVTGQNLVVDGGLVHN"
BASE COUNT          967 a         1242 c         1337 g          879 t
ORIGIN      
        1 gggcgccgct ccccccagtg ccaggcacgc tacagcgcac gcagccaagc cgggcgcgtg
       61 ccgccaagtc ttgcaagcta aatcggaaac agacatcagg acccaaagtt gtgccgccgg
      121 atgcatgatc accccgcagc aatgaaagtg tttgttggtg cgtgtgctct gcaaggtgca
      181 tatcgtacgc gcacgacgtc cattttagga cgatattgaa ttgaatacag gcccgggtcg
      241 agagggagtt tgcggtattc ctgccgaaag cgcgcccatc gggggtgaat catttcgcct
      301 gggccagcct ggaagaattg ggcctggctt tggccatgtg ccattcttgc agcaaggtca
      361 ggacgacggt caggctcatg aagattactg acgcccagaa aatggaattg gggtgaccat
      421 tatccagcat ccagccgaag atcatgggcc cggtggcccc gcccacattg aagccgctgg
      481 tcacgatgcc aaacgctttc ccttcggacc ccgctggcgc ggcggcgcgc acaagcatat
      541 ctcgcgaagg agtgatcacg ccggttaaaa accctatcgt gcccagcgta attgtcagtg
      601 caaggtcgtt tagcgatgtc atggcaacga tcgccgtgaa cacggctgcc aaaccaaacg
      661 ccacggcggc gaccagccca tgccttttgg tgcgatccgc cagtgcgccg ccgaaaagca
      721 cgccaaaggc actggcaaac aagaatgcgg tcaaggccgt atttgcccat gaaagcgtga
      781 cttcgtgtcc ttgcacgaag gcggcgacag agaatttctc gattgcgcct gtgctcaggc
      841 taagaagcgc gaacagcagc gtaagcacgc caatcatggg cgtgaagatc ggtggacgcc
      901 ttgatttggt gagcggttta ccggaattgg ccactacccg ggtctgagga acctttgata
      961 tgccggaacc gggtataaga atcaaggcag cgatgacaaa tccgatgacc gctgtgacgg
     1021 cgaaggcgat ccgcgggttg ctcagggcgg caatacccaa cagaaaggcc ggcgcgatgg
     1081 cggaacccag aaaccccgta aaggtatgta ttgaaaaggc tctgccaatg cggctggggt
     1141 taatgccttt ggagagcagc gaataatccg caggatggta aacgccattg gcgactcccg
     1201 cgagccccat ggcaatgagc aaacaggcat agttggggat cagcgccacg gcgacaaaac
     1261 agctactgcc aaggacaaga cccgacacca agacacgctt ggcgccgtaa tgatcgacgg
     1321 caaagccaag gggcgcctgc acgagcgcgg agacgatatt gaacacacta agcgcaagcc
     1381 caagttctat gaaccccacg ccaaagaact ggggcagaat cggcaacaag gcgggcagcg
     1441 ccatgatatg aaaatggctt gcaaaatgcg caagcgatac ctgcggcagc aacccgatgt
     1501 tgcgaatgga catgactaac cctgtttgta gccgatttga ctggccgtat acgggcgctt
     1561 ttgtggcgtt tgcgtattcc cgaagcctta atagtagccg accaaggggc caatctgtgg
     1621 tcgaatatgc cgcatcggag catttctgtg ctcaagtgcg acagtctgct agactgccta
     1681 cttgcccgtt cacagaaatg tccaatttag ggggttgaat gccgcaggcc aagaatctgt
     1741 ccaacctgaa tgggaaagtt gcgcttgtta ccggctcggc tatcggtatt gggcgcgcta
     1801 tcgctatccg gctggcacaa gacggcgcgg acctggcgct ttttgacatc aaccaagaag
     1861 aaacgcaaaa caccgctgcc atggtgcgcg ccatcggccg ccgttgtgaa gtcgttcgcg
     1921 gcgatgttgg cgattacgtc caggttcagc gggcggtcac tcatttcatt gcgtcgttgg
     1981 gcgccatcga cgtcgctgtc cacaatgcgg gaattacgtg tgttggtacg gttgcagaaa
     2041 cgtcgatcga agatttccat aaagtctttc gcgtcaacgt cgacggaatt ttttacggct
     2101 gcaaggcggt agtgccgcac atgatggagc ggggcagtgg aaagatcatc aatacctcgt
     2161 cctggctcgg caaggcaggc aaaccgaact atggggcgta tagcgcctcc aaggccgcgg
     2221 tcataggcct gactcaatct cttgcgatgg aggtggctcg ataccagatc aatgtcaacg
     2281 ctgtgtgtcc aggcgtgatc atcgagaccg gcatgcgcga cgaggcggat gccgcgtcaa
     2341 tcaggcaagg gctaccgacg gcgaagcagc gtgagacgga gataccgatc ggccgcgttg
     2401 ggttgcccaa tgacgtcgcg cgcgtggtgt cgtttctctc ttctgaccag tccgattaca
     2461 tgaccgggca ggcggtcaat gtcaccggtg gcctgtggat gcaataaacc ccctaccagg
     2521 ataatcatga cccgtaatca gattttgtgc gatgttaacg atggcgttgc cacggtcacg
     2581 ttaaacaatc tgcccctaaa tgtcgtcaca ctcgagatgt cgcgcgaatt gagcaaggtg
     2641 ctgcccgctt tggcggcgga ttccgctgtg cgcgcgcttg tcctcaccgg ggccggagaa
     2701 cgcgcattct gtgcggggtc tgacattagc gaatttccca atatgctggg tagcggcaag
     2761 atcgtaccca tgaagctggc tttcgaaaat caggtctatg ggacagtagc ggcatttcca
     2821 aagcccacca tcgccgcgat ctcgggtctg gcctatgggg gtgggcttga attggcggtg
     2881 tgttgcgatt ttattgtcat cgaagagcac acgcgtcttt gccttccgga gatcaagctg
     2941 ggcgtattcc caggcagcgg tggcacggtt cgcgtcacca ggcgaatcgg cgcaagccga
     3001 accaaggaaa tgatgttttt tggtgaaccg atcgatgcgc ccacagctct tttgtggggg
     3061 ctggtgaatc gggtcgttcc cgccggccag agccggcaca ctgcccgagc catggcgcag
     3121 gacttggcac gtcaacccaa tcaagctttg cagctttgca aggccgctat cgcgttatct
     3181 tgcgatacgc ccgaggacga agccatccgc gacacgatgg atctgagcga caaggtgttc
     3241 gccaccgagg attgcaaaga aggcgtcagg gccttctttg cgaaagaaaa gccgcatttc
     3301 aaacatcgat agcgaatcag tcgccgtgat tgcctgcggg tgctgacgcg ccaaagcgat
     3361 gcggtggccg gcctatggcg cctgctggcc tgcgattccc ggctgggcgg cgtttttccg
     3421 atgcgttgcg cggccgtgcg cgcggtggta aattctgcgt tagcaacacg gggtgtcccg
     3481 acaggatcgg ggctgagaag acacccgtcg aacctgattc agttcatact gacgaaggaa
     3541 tgttcgcgac gtggcagcgc gctgccggcg gcgggctctt tcgcctgact ccgagagata
     3601 tcatgcctgg ctcgcaacaa cccctatcac tgaatgaaca agtagtgctt gtcaccggag
     3661 gcgcccgggg cttgggcgcg cacatcgtac gtgcgttcca gcgtgaagga gcccgtgtgg
     3721 tcgtcaacta ctttcgcagt gccgggtcgg ccgcggcgct ggccgcccag aggccggatc
     3781 gtacacttgc gataatggcc gacgtccgcg acgacggcgc cgtgaaggcg atggtcaatc
     3841 aggcacaaca acatttcggc gcacaggtga ccaccgtcgt caacaacgcc ttgccggact
     3901 tccagttcaa tggcgatcag cgccaccagg ccgatgcctt gacatgggag gacatccacc
     3961 atcagatgga aggaactgtg cgtggagctt taaacactat ccaggcggcg ttgcccggca
     4021 tgcgaaaact ggggttcggc agagtgatca acgtgggcac gaacctcttt caaaacccgg
     4081 tcgtgcccta ccatgactac acagcggcca aggcggcgtt gctcgccttc acgcgtacgc
     4141 tgtcgcatga cctggggcct gaaggcatca ccgtcaacat ggtttcgggt ggcctgttgc
     4201 gcacgaccga tgcctccgcc gccacacccg aggccgtgtt cgagttcatt gccgccacca
     4261 cgccattgcg ccgtgtcacc accccggcag aatttgccga cgcgctgctg tttttcgctt
     4321 cgccatggtc gcgttcggtc accgggcaaa atctggtggt cgatggcggt ctggtgcaca
     4381 actgaatcgc ggggagagca cgctgcatct caatcttttc atatt
//
LOCUS       DATCXU010000421         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_601377,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000421 DATCXU010000000
VERSION     DATCXU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601377"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VIR04_09475"
     CDS             <1..911
                     /locus_tag="VIR04_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY9461020.1"
                     /translation="IAKIDEYSGRASQAGAGLILFPEAFVGGYPRGSSFGAVVGARTN
                     EGRDEFRRYWEAAIEVPGPECATMGKIAARHALYLVAGVIERHGGTLYCTVLFFAPDG
                     QLLGKHRKLMPTASERLIWGFGNGSTMPVFDTPLGKVGAVICWENYMPLMRAAYYAKG
                     IEIYCAPTADGRETWLPTMRHVAMEGRCFVLSCNLFARRSDYPENYSSSDGASDEIVS
                     RGGSCIIDPFGTVLAGPNFEGEALLTADIDRDAVARGKFDFDVTGHYARPDVFRLLVN
                     QTDVQPVSFTDEGAHNDAVSSAHKGA"
     gene            954..1901
                     /locus_tag="VIR04_09480"
     CDS             954..1901
                     /locus_tag="VIR04_09480"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY9461021.1"
                     /translation="MKILVLGAGGVGGYFGGRLAQSGANVSFLVREKRKQQLDTQGLR
                     IESHFGDATVPVITHLRTDIRPDYDLVVLTCKAYDLSDAMDTIEGALGDNTVVLPLLN
                     GVAHLELLNQRFGASRVWGGTAKIAATVTPDGVIRHLNQWHILTFGAQDKIVSAKVKT
                     FKAQLDKAGVTAVISPDITRDLWMKLVHLGTIATLTSLLRANAGEINRTPDGKALFQR
                     VLETNIQIASREGYPPDASFIASYRELFSQSDSIYEASLARDIEKGGPIESDHILGFL
                     LERCRAHGLPDDIPCLAYTHAKAYEQRRAANRLPARAAA"
     gene            1925..2299
                     /locus_tag="VIR04_09485"
     CDS             1925..2299
                     /locus_tag="VIR04_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9461022.1"
                     /translation="MTTATNFTAREEQSSHFTISSRKLVAQGSDVYVKEFTLAEKEEI
                     PWHSHSNIFDIFYCIQGHLRVERIEISTGNRQPDMELNVGDSGKVDVGTAHRPFNPGP
                     GPCRFINVQGVGTYDYLPYTPV"
BASE COUNT          502 a          708 c          664 g          477 t
ORIGIN      
        1 ccatcgccaa aattgacgaa tacagcgggc gcgccagcca ggcgggcgcg gggcttattc
       61 tatttcctga agccttcgtg ggcggctatc cgcgcgggtc aagctttggc gccgtcgtcg
      121 gcgcgcgcac caacgaagga cgtgacgaat ttcgccgcta ctgggaggcg gctatcgagg
      181 ttcccggacc cgagtgcgcc accatgggaa aaattgccgc gcgccacgcc ttgtacctgg
      241 tcgcaggcgt gatcgagcgc cacggcggca ccctgtattg cacggttctg ttctttgcgc
      301 ccgacgggca gttgcttggc aagcaccgca agctaatgcc taccgcttcg gaacggctta
      361 tttggggctt tggcaacgga tccaccatgc cggtgtttga tacccccctg ggcaaggtcg
      421 gcgctgtcat ttgctgggaa aactatatgc cgttaatgcg cgcggcctac tacgcaaagg
      481 gcatcgagat ctactgcgcg ccaacggcgg atggccgcga aacctggctg cccaccatgc
      541 gtcatgtggc tatggaaggg cgctgcttcg tgctgagctg caacttgttc gcgcgccgca
      601 gcgactaccc ggaaaattac agcagctccg atggcgccag cgatgaaatc gtatcgcgcg
      661 gcggcagttg catcatcgat cccttcggca cagtgctggc gggaccgaac tttgaaggcg
      721 aggcgctgct tacggctgac atcgatcgcg atgctgtcgc acgcggcaaa ttcgacttcg
      781 acgtgaccgg ccattatgcg cggccggatg tctttcggct gctggtcaac cagaccgacg
      841 tgcaacccgt aagcttcact gacgaaggcg cacacaacga cgcggtttcc tccgcgcaca
      901 aaggcgcctg acaggcgtct cactctttca tcatttttcg acgagggcat tacgtgaaga
      961 tactggtatt gggagcagga ggcgtcgggg gctattttgg aggccgcctg gcgcaaagcg
     1021 gcgcaaacgt aagctttctg gtgcgcgaaa aacgcaagca acaacttgac acgcaggggt
     1081 tgcgcattga aagccacttt ggcgacgcaa cagttcctgt catcacacat ctgcgcacgg
     1141 acatccggcc cgactatgat ctggttgtgc tgacctgcaa agcctacgat ctctccgatg
     1201 ccatggatac aatcgaaggc gctcttggcg ataacacggt cgtgctgccg ctacttaacg
     1261 gcgtcgccca tctggaattg ctcaatcaac gtttcggcgc ttcgcgcgta tggggcggga
     1321 ccgccaagat cgctgctacg gtaacgcccg acggcgtcat ccgccatctg aaccaatggc
     1381 acatcctgac ttttggcgcc caggacaaga tcgtgtcagc caaggtaaaa acattcaaag
     1441 cgcagctcga taaagcgggc gttacggctg tgatctcacc ggacattacg cgcgacctgt
     1501 ggatgaaact ggtgcatctg ggcacgatcg ccacgcttac ctcgctgttg cgcgccaatg
     1561 ccggcgaaat caaccgtacg ccggatggca aggccctgtt tcaacgcgtg ctggaaacga
     1621 atattcaaat tgcgagccgt gaaggctacc cgcccgacgc ctcttttatc gcaagctatc
     1681 gcgaattatt ttcgcaatcc gattccatct acgaggcttc gctggcgcgc gacatcgaaa
     1741 aaggcgggcc catcgaatcg gatcacattt tgggtttcct gctggaacgc tgtcgcgcac
     1801 acggtctgcc cgacgatatc ccttgtctgg cctacacgca cgccaaggct tatgaacagc
     1861 gccgcgctgc gaatcgactg cccgcgcgcg ccgctgcctg attcaactca tccgcgagga
     1921 tcgtatgact accgcaacaa acttcaccgc tcgggaagag caaagctctc acttcacgat
     1981 atcgtcgcgc aagttggtgg ctcagggtag cgacgtttac gtcaaggaat tcacgctggc
     2041 cgaaaaagag gaaatcccct ggcatagcca ttcgaatatc ttcgacattt tctactgcat
     2101 tcaagggcat ttgcgcgtag agcgcattga aatttccacc ggaaatcgac aacccgacat
     2161 ggaactcaac gtaggcgact cgggcaaagt ggatgtgggt acggcacacc ggccattcaa
     2221 tcccgggccc gggccttgcc gcttcatcaa cgtgcaaggt gtcggcacat acgactacct
     2281 gccgtatacg cctgtttgat atcaagcgtc ctccttacgc acgaagacta tccgcgagga
     2341 ctcccaccga c
//
LOCUS       DATCXU010000422         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_438268,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000422 DATCXU010000000
VERSION     DATCXU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438268"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VIR04_09490"
     CDS             <1..656
                     /locus_tag="VIR04_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY9461023.1"
                     /translation="APSVHIRLSVSLPLALPETSGYRHMAIHPYPRRLTKMKTMADGT
                     QWLMRPIRPEDAQPLQTFMRNLSDESRYMRFVSMLRELTPRMLARYTRIDYDRELALV
                     ATVQAPNLRHRGHPQEQIAGFAHYLRNGDGSGAEYALVIGDDWQRRGIGAELMRGLIE
                     VAREQGLTYIEGAVLASNRPMLALMARLGFQNDGYYQDPTMRRVWFDLGKTSRPAQQ"
     gene            complement(665..1345)
                     /locus_tag="VIR04_09495"
     CDS             complement(665..1345)
                     /locus_tag="VIR04_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEY9461024.1"
                     /translation="MTEFNTPLLDCIETETAPNPTHSVIWLHGLGADGSDFAPLVPQL
                     HPVNRPAIRFVFPHAPVRPITINNGFAMRAWYDVFPPDPIRREDGPGIRASQQSIEAL
                     IARENGRGIPTGKIVLAGFSQGCALTLHTGLRLNQKLAGMIGLSGYLPLADSFTRERH
                     SANQDTPIFLAHGTLDPVVNFSRAQATRQILTDLDYPVTWKTYAMPHAVCPEEIVDIA
                     GFLRQILI"
     gene            complement(1342..1890)
                     /gene="yjgA"
                     /locus_tag="VIR04_09500"
     CDS             complement(1342..1890)
                     /gene="yjgA"
                     /locus_tag="VIR04_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY9461025.1"
                     /translation="MTIDATEHPESGYERPSKSHVKREMLALLDLGKQLIELPDEKLK
                     QLPLAERLYDAIRQAQRTPGREGRRRQIHYVGKLMRDAQADEIRAQIDLWENGSRAQT
                     RAMHRIEALRDLLLSDDGALTTLLNEYPQVDSQAMRALIRAARNEAKQNTELTQGQEP
                     ARKHYRALFQTLKALDATKEEN"
     gene            1952..>2389
                     /locus_tag="VIR04_09505"
     CDS             1952..>2389
                     /locus_tag="VIR04_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY9461026.1"
                     /translation="MTDHSTLHLSIVENQSRFRELVHDSLRYAKSIGASDAVAEVSES
                     GGLDVSVRNKDIETVEQTHDRSLDVTVFAGQSRGSASTSDFSTKALQETVQAAWHIAR
                     YTAADPAAGLPDTDLLAFDYPDLELYYPWPISTDEAAALALQAE"
BASE COUNT          463 a          672 c          711 g          543 t
ORIGIN      
        1 cggcgccttc ggttcatatt cgcctgtcgg tttcgctacc gctggctctg cccgagacgt
       61 ccggttaccg gcatatggcg attcacccat atccccggcg cctgacaaaa atgaagacca
      121 tggccgacgg cacacagtgg ctgatgcgcc cgatccgccc tgaggatgcg cagccgctgc
      181 agacatttat gcgcaacttg tcagacgaat cgcgctacat gcgttttgtg tcgatgttgc
      241 gcgaactgac tccgcgcatg ctggcccgtt acacccgcat cgattatgac cgcgaactgg
      301 cgctggttgc caccgtacaa gcgcccaacc tgcggcatcg cgggcatccg caggagcaga
      361 ttgcgggttt cgcgcattat ctgcgcaacg gtgacggcag cggcgccgag tatgcactgg
      421 tgatcggcga cgactggcaa cgtcgtggca taggtgctga gcttatgcgg ggcttgatcg
      481 aggtggcgcg cgaacagggc ttgacgtata tcgagggggc ggtgctggcc tcgaatcgtc
      541 ccatgcttgc cttgatggcg cgcctgggct tccaaaacga cggatattat caagatccca
      601 ccatgcgcag ggtctggttc gatctgggta aaacgagccg cccggcgcag caatgaacgt
      661 ccggtcagat cagaatttgt ctcaggaaac ccgcgatatc cacaatttct tccgggcaca
      721 ccgcatgcgg catcgcgtag gttttccatg tgaccggata gtccaggtct gtcagaattt
      781 gtcgcgtcgc ctgagcacgt gaaaaattca ccacaggatc gagcgtgcca tgagccagaa
      841 aaattggcgt gtcctgattg gcggaatggc gctcgcgggt gaatgaatcg gccagtggca
      901 aatatcctga cagcccgatc atccccgcaa gcttttggtt caggcgcaag cccgtgtgca
      961 aggtcagtgc acagccttgt gaaaaccccg ccagaacgat ttttccggtt ggaatgccgc
     1021 ggccgttctc gcgcgcgata agggcttcga tggattgctg tgaagcgcgt atgcccgggc
     1081 cgtcttcgcg tctgatcgga tcgggcggga aaacgtcata ccatgcccgc atggcaaatc
     1141 cgttgttgat ggtaatgggc cggacgggcg catgcgggaa tacaaagcgg attgccggcc
     1201 tgttgaccgg gtgcaattgc ggcacaagcg gcgcaaagtc gctgccgtcg gctcccaggc
     1261 cgtgcagcca gatcacgcta tgggtcggat tgggagctgt ttcggtttca atgcagtcga
     1321 gcaggggggt attgaattcc gtcaattttc ttcctttgtc gcgtccagtg ctttaagagt
     1381 ctggaacagc gctcgataat gcttgcgggc gggttcctgg ccttgcgtca attcagtgtt
     1441 ttgtttcgct tcgttgcggg ccgcccggat cagtgctcgc atggcttgac tatcgacctg
     1501 cggatactcg ttcagaagcg tcgtcagcgc accatcatca gacaataaca gatcgcgcag
     1561 cgcttctatg cgatgcatcg ctcgtgtttg ggcgcgcgac ccgttttccc acagatcgat
     1621 ctgggcgcgg atctcgtctg cctgggcatc gcgcatcagt ttgccgacgt aatggatctg
     1681 acgccggcgc ccctcgcggc cgggggtgcg ttgagcctgg cgaatggcat cgtagagcct
     1741 ttccgccagc ggcagttgtt tcagtttttc gtcgggtagt tcaataagct gtttacccaa
     1801 gtccagcaac gcaagcatct cgcgcttgac atgcgacttg cttgggcgct cgtaaccgct
     1861 ttcagggtgc tccgttgcgt cgatagtcat aaaaatagtg ggttcagtcc attaattggc
     1921 tatgataacc gcctaacctg aaagagcagt aatgaccgat cactctactt tacatttatc
     1981 catcgttgaa aatcagtcgc gatttcgtga actggtgcac gattcgctgc gttatgcgaa
     2041 atccatcggc gcctccgatg ccgtggcgga agtgtctgaa agcggcggcc tggacgtatc
     2101 ggtgcgcaac aaggacatcg aaaccgtcga acaaacgcat gaccgttccc tggacgtaac
     2161 cgtgtttgcc ggtcaatcgc gcggctcggc ttcgacttcc gacttttcaa ccaaggcgct
     2221 gcaggaaacg gtgcaggctg cgtggcatat tgcgcgatac actgccgccg acccggcggc
     2281 tggtttgccc gataccgatt tgctggcgtt tgattatcct gatttggagc tgtactaccc
     2341 ctggcccatt tcgaccgatg aagccgccgc gctggcattg caggcggaa
//
LOCUS       DATCXU010000423         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_368189,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000423 DATCXU010000000
VERSION     DATCXU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368189"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VIR04_09510"
     CDS             complement(<1..747)
                     /locus_tag="VIR04_09510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461027.1"
                     /translation="MTFGSVLLRLITAIAVTLIMGPIIVVLIVSFSSGELLTFPLPGF
                     SLRWFYNFFSLPEMRNAFALSLILAFTSATAAVLLGMMGAIYTSRRDSWLSHILQLLF
                     ITPLVFPTIVLGLALLLLYRTFGMWITPGLFIAHVIVCLPYAFRAVLASLQSFDVSLE
                     DAGQSLGAGPIRSFTLITLPIIWPGILSGWLFAFVVSFGELNVGLFLTGPGVVTLPIE
                     IFSYLQFQGSQLVVAAASALQILVIIFILLV"
     gene            complement(744..1607)
                     /locus_tag="VIR04_09515"
     CDS             complement(744..1607)
                     /locus_tag="VIR04_09515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461028.1"
                     /translation="MNLRSKSNLFWATAAPPLVVLFIFFIVPLAYLFFVSFMTNSQFQ
                     LYELKPTLDNYLQIASDPFYLLIIKRTLLATGVVLIACLMLGYPAALYASRLSPRGRL
                     IMLMLLTLPLMVSNVVRAYGWLSILGRNGVINGGLQSLGLIKFPIHMLYSFEAVTLGL
                     LTILLPFMIITITNSLAAIDKSYTEAAESLGAGPWRTFMRVTLPLSSPGVTSGLMLVT
                     FLMLSAYVTVALLGGPRYKLLVSLVFDSVTTFRWPRAAALSFVLLAIAMVIAVLIQVL
                     LRPRRAPRRTR"
     gene            complement(1616..2701)
                     /locus_tag="VIR04_09520"
     CDS             complement(1616..2701)
                     /locus_tag="VIR04_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461029.1"
                     /translation="MNATVPAMRQPDVRFDNVEKHFGAMVALHKLDLDIDRGVFFSLL
                     GPSGCGKTTTLRLIAGFEQPTHGDVYIRQKCVTGLPAYKRNFGMVFQNFALFPHMTVS
                     ENVAFGLKMRHVDQSSIKEKVLKVLDLVSLGKYGDRYPSQLSGGQQQRVALARAIVFE
                     PDVLLLDEPLSALDKMLREQMQVELRQLQRRLGTTTIFVTHDQEEALTMSDQVAVMKE
                     GQIQQAGAPRDIYEQPATEFVATFLGASNILESTIQGRDGDQIVVDMAGTPLKVNSRQ
                     ITQTSPGQMLKLALRPEKIRLSSNGLLRARLKEAIYRGAKTYLEMDANGVSIVAHIPN
                     STALPFSLEPGCELQLAWDDGSLVPLA"
     gene            complement(2698..>3466)
                     /locus_tag="VIR04_09525"
     CDS             complement(2698..>3466)
                     /locus_tag="VIR04_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461030.1"
                     /translation="DQHRLSETLGRYAQYALPSRLLIDNPLERARYETLMHGKPAILQ
                     GHATQGRIIVFSPHPEMGDFVRKGIALDGYIRHYLPLRGFKTMDETLCFYAQEDCLGF
                     RLILNALVRLGVFNNQPRPTIGAAAESAQMIRDDLSAALKNIRAIRQCKSEYLLSRLA
                     TDEPDTALLITHELRRQQEQWLAIGDRLDARITDEDADPNTLKELAFALHDAAAFLES
                     APDRSIADGVVSLELPIRLTAACVRLLHCDEILKETL"
BASE COUNT          850 a          910 c         1005 g          701 t
ORIGIN      
        1 taccagcaag atgaagataa tgaccaggat ttgcagcgcc gatgcggcgg ccacgacaag
       61 ctggctgccc tgaaactgca ggtagctgaa aatttcgatt ggcaaggtga ccacgcccgg
      121 gcctgtgagg aaaagaccca cgtttagctc gccaaacgag accacgaatg cgaacagcca
      181 tcctgacagt atgcctggcc agatgatggg cagcgtaata agcgtaaacg agcgtatggg
      241 tcccgcgcca agactttgac cggcatcttc cagcgagacg tcgaaacttt gcagcgaagc
      301 cagcacggcc cggaatgcgt acggcagaca tacgattaca tgcgctatga acaggccagg
      361 cgtgatccac attccgaacg tgcgatagag taaaagtagt gccagcccaa gaacaatcgt
      421 ggggaaaacc agcggtgtga taaaaagcag ctgcaatatg tgggacagcc aggagtcgcg
      481 ccgcgacgtg taaatggccc ccatcatgcc gagcaggacg gcggcggtcg cggacgtaaa
      541 cgccaggatt agactcagcg cgaatgcatt gcgcatttcc ggcagggaaa agaagttgta
      601 gaaccagcgc aaagaaaaac cgggcagcgg gaaggtcagc agctcgccgg aactgaagga
      661 cacaatcagg acaacaatga tcgggcccat gatcagtgtc acggcaatcg ccgtgataag
      721 gcgcagaagc accgagccga atgtcaacgc gttctccttg gcgcccggcg cgggcgaaga
      781 agcacctgaa tcaacaccgc aatgaccatt gcaatggcaa gcaaaacaaa tgacaaggcg
      841 gcggctctgg gccagcggaa cgtcgtcacg ctgtcgaaga ccagactgac caatagtttg
      901 tagcgcggcc ctcccagcaa ggcgaccgtg acgtaggcgc taagcatcag aaatgtgacg
      961 agcataaggc ctgatgtcac cccggggctg gaaagcggca aggtgacgcg catgaatgtt
     1021 cgccacggcc cggcgcccag ggattcggct gcttcggtat aacttttgtc tatcgccgcc
     1081 agcgagttcg tgattgtgat gatcatgaag ggcaacagga tggtcagcag ccccaacgta
     1141 accgcctcga acgaatagag catatgaatg ggaaatttga tcagacccag gctttgcaaa
     1201 ccaccgttga tcaccccgtt tcgacccaaa atgctaagcc agccataggc gcgtacaaca
     1261 ttgctgacca taagcggaag cgtcaggagc atcagcatga tcagccggcc gcgtggagac
     1321 aaccgcgagg catacagcgc tgccgggtag cccaacataa ggcaggcaat cagcacaacg
     1381 ccggtggcca gcagggtgcg cttgatgatg agcaaataga atggatcgct ggcgatttga
     1441 aggtagttgt ccagcgtggg cttgagctcg tagagctgaa attgtgaatt ggtcatgaag
     1501 ctgacgaaaa acaggtacgc cagcggcacg ataaaaaata tgaacagcac caccaggggt
     1561 ggcgccgcgg ttgcccagaa cagattggat ttggagcgca agttcataaa tgaattcagg
     1621 ccaaaggcac caggcttccg tcgtcccagg ccaattgcag ttcgcacccc ggttcaagag
     1681 agaagggaag tgccgtggaa ttgggtatgt gcgcgacaat ggaaaccccg tttgcatcca
     1741 tctccagata ggtctttgcc ccgcgataga tggcttcttt caagcgcgcg cgtagaagac
     1801 cgtttgaact cagacggatt ttttcgggtc gcagggccag tttgagcatt tgtccgggcg
     1861 atgtctgcgt tatttgacgc gaattgacct ttagcggtgt gccggccatg tcgacaacaa
     1921 tctggtcacc gtcgcgacct tgtatggtgc tttccagaat gttgctggcg cccaggaatg
     1981 tagcgacgaa ttctgtggcc ggttgttcgt aaatgtcgcg aggagcgcct gcttgctgga
     2041 tttggccttc tttcatgacg gccacctggt ctgacatggt aagtgcttct tcctgatcgt
     2101 gggtcacaaa tatagtcgtg gttcccagcc tgcgctgcag ttgtcgcagc tccacctgca
     2161 tctgttcgcg cagcattttg tccagagcgc taagtggttc atccagtaac agaacatctg
     2221 gctcgaaaac aattgcccgc gccagggcca cgcgctgctg ttgcccgcct gacaactgac
     2281 tgggatagcg atcgccatat tttcccaagg acacgaggtc cagaaccttt aaaacttttt
     2341 cttttatgct ggactgatca acatgccgca ttttcagccc gaacgcgacg ttttcggaga
     2401 ccgtcatgtg tggaaacagc gcgaaattct ggaataccat gccaaaattg cgcttgtagg
     2461 ccgggagtcc ggtaacgcat ttttggcgga tatatacgtc cccatgggtg ggttgctcga
     2521 aaccggcgat tagcctcaag gttgttgtct tgccgcagcc gctcgggccc aatagcgaaa
     2581 aaaagacgcc ccggtcaatg tcgagatcaa gtttgtgcaa cgccaccatg gcgccgaaat
     2641 gtttttcgac gttgtcgaat ctcacatcgg gctggcgcat cgcgggtaca gtcgcgttca
     2701 caaagtttcc ttaaggattt catcgcaatg taaaagacgg acgcaggctg cggtcagacg
     2761 aatgggcagt tccagactca cgacgccgtc cgcgatggac cgatcgggtg cgctctcaag
     2821 aaacgcagcg gcgtcatgca gggcaaatgc cagttctttc aacgtattcg gatcggcgtc
     2881 ttcgtcagtg atgcgggcat ccaggcgatc gccaatggcg agccactgtt cttgttgccg
     2941 tcgcagttcg tgcgtaataa gcaaggctgt atccggctcg tccgtggcaa ggcgcgacag
     3001 cagatattcg gacttgcact ggcgaatcgc ccggatattt tttaatgccg cgctcaagtc
     3061 atcgcggatc atttgcgctg attccgcggc cgcgccgata gttggcctgg gctggttatt
     3121 gaatacaccc agtcgcacaa gcgcattaag aatcagacga aagccaaggc agtcttcctg
     3181 ggcgtagaaa cacagggtct cgtccatggt cttgaaaccg cgtaatggca gatagtgacg
     3241 tatatatccg tccagagcaa tacccttgcg cacaaagtcg cccatttcgg gatgaggcga
     3301 aaagacgatg atccgaccct gcgttgcatg accctggagt atggctggct tgccatgcat
     3361 aagggtttcg tagcgtgcgc gttcaagcgg gttgtcgata agcaagcggc tgggcaaagc
     3421 atattgtgcg tagcggccca gagtttccga caaccgatgc tggtcg
//
LOCUS       DATCXU010000424         9737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_123989,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000424 DATCXU010000000
VERSION     DATCXU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123989"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VIR04_09530"
     CDS             complement(<1..242)
                     /locus_tag="VIR04_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY9461031.1"
                     /translation="MRAAVFGFLLLLAACAVGPDYQRPDVAVGQSYKEAQGWKLAQPG
                     DTELRGDWWKTYSDPVLDGLMDTLLRANLSITQAEA"
     gene            complement(286..3396)
                     /locus_tag="VIR04_09535"
     CDS             complement(286..3396)
                     /locus_tag="VIR04_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9461032.1"
                     /translation="MRFFTIFVMRPVTTTLLCAACVLTGMLAFHFLPVAPLPQVDFPI
                     ISVSASMAGASPETMASSVATPLEQSLGSIAGVTDMSSSSTEGSTRIRLTFDFSRDID
                     SAARDVQAAINAARNLLPSSLRSNPTYHKVNPASAPIMVLALTSDTVRQGQLYDLAST
                     VIAQKLSQVEGVGEVTVGGSSLPAIRVSLNPQALTNAGVALDDVRTALVNANTMRPNG
                     SVGNDVAHWQLSSGQQLMNAAQYRPLIVAWRNGSPVRLLDVARVDDGVEDIFNTGFFN
                     DKAAVLLIVRRQSDANIIETVDAIREQLPVFRDMLPAQVALTVAQDRTPSIRASLSEA
                     EITLIIAVLLVVLVVLVFLGNIRAALIPSLAVPASLITTFSLMLWFGFTLNTISLMAL
                     IVATGFVVDDAIVVLENIMRHMERGVPPLRAAIRGVKEVGFTVLAMSLSLIAVFIPIL
                     LMGGIPGRLFKEFAVTLSVTILVSLAISLVLTPMMCAHLLRPLPAVSKAPGVLRRFFQ
                     SVGGVFANGYQKSLGWALAHGRFMMLLLGITIGLNFYLYSVIPKGFFPQQDTGQLLGF
                     FRVDQGTSFTAMQAKLDGFRKVLMKDPAIDSVTAFAGGRGGSNSSFMLIQLKAIGQRT
                     ATADQVVNRLRSQFTNAPGARLFLVPQQDIRVGRQSSSGSYDYTLMTGELDLLKTWMP
                     QVQKAMAGLPELVDVDTDVEDKGRQVELVIDRDAATRLGVNMSLIASTLNNSFSQRQI
                     SVLYGPLNQYHVVMGVDPRYSQDAESLRQVQVVTAGGQRVPLSAFTHFVDSNAPLSVE
                     HQGLFASDTISFSLAPGVSLEQATRAIDSAVARLGLPTDQIQAGFLGTAQLLQGALSQ
                     QPWLILAALITMYIVLGILYESYVHPITILSTLPSAGIGALLALILFHKEFSLIALLG
                     VFLLIGIVKKNAIMMVDFALQAERKHNLSSRDAIYMACLTRFRPIMMTTASALFGALP
                     LVLATGAGVELRQPLGLTIVGGLILSQVLTLYTTPVVYLYLDQFRLWASRRRRRKTGL
                     VPNSPV"
     gene            complement(3393..6500)
                     /locus_tag="VIR04_09540"
     CDS             complement(3393..6500)
                     /locus_tag="VIR04_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY9461033.1"
                     /translation="MNLSRLFILRPVATTLAMVALFIAGLLAYRLLPVSALPEVDYPT
                     IQVVTLYPGASPEVMTALVTSPLERQFGQMPGLTQMSSSSSGGSSMITLQFGLNLAMD
                     VAEQEVQAAINAASNLLPSDLPTPPLYNKVNPADTPVISLAITSPTLPLYQVRDLIDV
                     RVAQKLSQISGVGLVSIAGGQRPAVRVQVNPQALAAHNLTMSNVRTTIVAANVNQPKG
                     NLDGPQRATTINANDQLSSVQDYDRLILTYEKGAPLRLGDVASTRLDAENIRQAAWIG
                     RTPAVLLNIQRQPSANVIDVVDRVKALLPSLRQSLPVGVDIAVATDRTQTIRDSISHV
                     EFELLLAIALVVMVTFVFLRSWTATFIPSVVVPLSLVGTFGIMYLVGFSINNLTLMAL
                     TIATGFVVDDAIVMIENIARYIEAGDRPLQAALKGAAQIGFTLVSLTLSLIAVLIPLL
                     FMSDVIGRLFREFAITLAVAILMSLLISLTLTPMMCARLLRPESEMKLGRFQRKTGAW
                     IKALIDAYDQALTWVLAHQRLTLWVALGTFLFTGLLYVLVPKGFFPQQDTGLIQAISE
                     APQTVSFQAMAQRQQEAVDKILQDPDVSSVSSFIGIDGTNSTLNTGRMQIALKLSSER
                     GADSRQIIDRLGTALQSVQGIKVYMQPVQDLTIDDQISRTQYQMSLSGPSESVLGQWV
                     PQVVKKLQALPQLVDVADNLQNGGLQTTLNIDRDAAARLGVTTAAIDEALYDAFGQRL
                     ISTIFTQSTQYRVVLEVAPQYRQTPASLSQIYVPTTSGAPVPLTSLAQLVQSNTLLSI
                     ERVDQFPAAMISFNLAPGVALSDAVQAIDRAQASLGMPISIQSKYQGAARAFQASLSS
                     TLWLLLAAVAAMYIVLGILYESYIHPVTILSTLPSAAIGALLALMLSHTELDMIGVIG
                     IILLIGIVKKNAIMMIDFALDAERHRGLSPQAAIHDAALLRFRPILMTTLAALFSALP
                     LMFSTGTGAELRQPLGLVMVGGLLCSQFLTLFTTPVIYLMFDNLSRRTRRGRSENKLG
                     TQALP"
     gene            complement(6510..7736)
                     /locus_tag="VIR04_09545"
     CDS             complement(6510..7736)
                     /locus_tag="VIR04_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY9461034.1"
                     /translation="MSDSSAEFNRPRRGRIWTSVLIIVVLLAAGYWLIGRKDSVKQRA
                     TNSARASAMASAAVPVKIATVVNGRINVTLKTIGTVTAFNTVTVQSRVAGPLRKVAFI
                     DGQKVRQGDLLAQIDPDTYQVQLEQALGTQKQNQAQLQNARRDLQRYQLLFKQNSIAK
                     QQVDAQAALVQQLQGTEKSDQAAVDSAKLQLDFTRITAPISGRLGLRMVDQGNLISTT
                     STSGLVVITQTQPISVIFTLPQAQLPEVLAQRAAGTRLAVDLYDRDDRNKIASGELMA
                     IDNQIDVATGTVKLKARFANEDESLFPNQFVNVHLHVSSNDAALLAPTMAVQQGSIGP
                     FVYLVNPDDKVHVQPVTTGTVDGQNVAIKSGLSAGQRVVTEGLDRLREGATVSVVAPA
                     ASATATPPVPAGHSAH"
     gene            complement(7850..8632)
                     /gene="zapD"
                     /locus_tag="VIR04_09550"
     CDS             complement(7850..8632)
                     /gene="zapD"
                     /locus_tag="VIR04_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY9461035.1"
                     /translation="MPALKKVYGHVVLYEYPCNERVRALLRVEYLFDRLFFFAKGEDV
                     HLQQITVATLFDLLDICDRTDLRGAVLQDLERQRGALSALRQHPSVDVRTLEAMLQEI
                     QSASTELSAQGRIGQVLRDNEWLASLRGRITVPGGTSQVDMPSYYAWQIKPLATRLHD
                     MQQWILPFLPLYRGLALILRLLRKSSDEISAEAKQGAYQEMLSGKTFQLLRVWVDPGQ
                     GVFPEMSANKYVIWVRFATQDQELKPQPVTRDIPFKLARCNV"
     gene            complement(8653..9264)
                     /gene="coaE"
                     /locus_tag="VIR04_09555"
     CDS             complement(8653..9264)
                     /gene="coaE"
                     /locus_tag="VIR04_09555"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391306.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY9461036.1"
                     /translation="MYKIGLTGGIGSGKSQVAGLFKDWGAAIVDTDVIAHDLTRPGGP
                     AIGPLRRVFGPEMIADDGSLDRELMRELVFANPAARGQLEAIIHPMIQVETDRQADHA
                     RGCYLVFVVPLLVESGRWRERVDRICVVDCDPETQRARVQARSGLTPEAIARIISTQA
                     SREARLAVADDVLLNDGQTTLSQLAQRARALHDVWVAQATRYR"
     gene            complement(9278..>9737)
                     /locus_tag="VIR04_09560"
     CDS             complement(9278..>9737)
                     /locus_tag="VIR04_09560"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY9461037.1"
                     /translation="FAGLVAASAIMLVLALVDAATMLLPDALTFPLLWLGLACAWVRG
                     PAFLQDSVAGVMAGYCFLWLLFWLYWHIRNRQGMGQGDFKLLAAQGAWVGLERLPYVL
                     LAACLLGVLFACLRQKNLNLSASYPFGPFLAISGMGVLLAGPEVHFYFWQ"
BASE COUNT         2293 a         2902 c         2689 g         1853 t
ORIGIN      
        1 ttggcctcgg cttgcgtaat gctcaggttc gcccgcagca atgtgtccat aagcccgtca
       61 agcacaggat cgctgtaggt tttccaccag tcgccgcgca gctcggtgtc gccgggctgt
      121 gcaagcttcc atccctgagc ctctttataa ctttggccca cggccacatc gggccgctga
      181 taatcaggcc cgaccgcaca ggcggctagc agtaacaaaa acccgaaaac cgctgcacgc
      241 ataaaaccgc gaaccccgga aaccgtcatg tcagatgccc catttttaaa ccggtgaatt
      301 tggaacaagg ccggttttcc ggcgccgcct gcggctcgcc cataggcgga attggtccag
      361 gtacaaataa acgactggcg tggtgtacaa agtgagcacc tggctcagaa tgaggccgcc
      421 cacgatagtc aggcccagcg gctgacgcag ttcaacgccc gcgcccgtgg ccagcaccaa
      481 tgggagagcg ccaaacaagg ccgacgccgt tgtcatcatg atgggccgaa aacgcgtcag
      541 acaagccata tagatggcat cgcgcgagga cagattgtgc ttgcgctcgg cttgcaaggc
      601 aaaatcgacc atcatgatgg cattcttctt aacaatcccg atcagcagaa aaactcccag
      661 caaggcaatc agcgagaact ctttatggaa cagaatcaat gccagcaaag cgccaattcc
      721 ggcggacggc aaagttgaga gaatggtaat gggatgaacg tagctttcat acaaaatgcc
      781 caggacgata tacatcgtta tcaacgcagc cagaatcagc cacggctgtt gcgacaatgc
      841 gccttgcagc agttgcgccg tccccagaaa cccggcctga atctgatctg ttggcaaacc
      901 aaggcgcgcc acggcactgt caattgcgcg agttgcctgt tcaagcgata cgcccggcgc
      961 caggctgaac gatatcgtgt cggaggcaaa caagccttga tgctccacgc tgagcggcgc
     1021 gttgctgtcc acaaaatgag tgaaggcgga caatggaacc cgttgccctc ccgctgtaac
     1081 gacctgcacc tgcctgagcg attcggcatc ctgcgaataa cgagggtcga cgcccatgac
     1141 tacgtgatac tggttcaacg gtccatacag caccgatatc tggcgctggc tgaacgaatt
     1201 attcaaggtc gacgcaatca gagacatgtt tacccccagg cgcgttgcgg catcgcgatc
     1261 gatgaccagt tcgacctggc ggcccttatc ttcgacgtca gtatccacgt cgaccagttc
     1321 gggcaatccg gccatcgcct tttgcacctg cggcatccac gtcttgagca aatcaagctc
     1381 gcctgtcatc aaggtgtaat cgtacgaccc tgaagaactc tgacggccaa cgcgaatatc
     1441 ctgctgcggc accagaaaca ggcgcgcgcc cggcgcattc gtaaactggc tgcgcaaacg
     1501 atttacgacc tgatccgccg tcgccgtacg ctgaccaatg gctttcagtt gaatcagcat
     1561 gaaactggaa ttgctgcccc cgcgccctcc cgcgaaggct gtaacgctgt caatcgccgg
     1621 atccttcatg agcaccttgc gaaaaccatc aagcttcgct tgcattgcgg taaacgacgt
     1681 tccctgatcc acccggaaaa agcccagcaa ctggcctgta tcctgctgcg gaaagaaccc
     1741 tttcggaatg accgagtaca gataaaaatt caggccgatc gtaatgccca gcaacaacat
     1801 catgaaccgg ccgtgcgcca gcgcccagcc cagtgatttt tggtatccgt tggcgaacac
     1861 gccgcccacg gactggaaaa accggcgcaa aacgccaggc gcttttgaaa cggcgggcaa
     1921 gggtcgcaat aaatgggcgc acatcatagg cgtaagcaca agcgagatcg ccagcgaaac
     1981 aagaatcgtc accgacaatg tgaccgcgaa ttccttgaac aaacgccccg gaattccgcc
     2041 catcaacagg atcggaataa agacggcaat cagggacagg ctcatcgcca atacggtgaa
     2101 ccctacttct ttcaccccgc ggatggcggc gcgcaatggc ggaacgccgc gctccatgtg
     2161 gcgcatgatg ttttccagca cgacaatggc gtcgtcgacc acgaagccgg ttgcaacgat
     2221 cagcgccatc agcgagatcg tattaagcgt gaatccaaac cagagcatca ggctgaacgt
     2281 ggtgattagc gaagccggaa cggcaaggct gggaatgagc gccgcccgga tattgcccag
     2341 gaacaccagc accaccagca ccaccagcag taccgcaata atcaaagtaa tttcagcttc
     2401 gcttaaagat gcgcgaatgc tgggggtgcg gtcctgtgcc acagtcagcg caacctgggc
     2461 cggaagcatg tccctgaata caggcaattg ttcgcgaatg gcgtcgaccg tttcgataat
     2521 gttggcatcc gactgacgcc ggacaatgag cagtacagcc gctttgtcgt taaaaaaacc
     2581 tgtattgaaa atatcttcga cgccatcgtc gactcgcgcc acatccagca ggcgaaccgg
     2641 cgagccgttg cgccaggcca caataagcgg ccggtactgc gccgcattca tcagttgttg
     2701 gcccgagctg agttgccaat gggccacatc gtttccgaca gatccgttgg gacgcattgt
     2761 gttcgcattg accagggcgg tgcgtacgtc gtcaagcgcc acaccggcat tggtcagcgc
     2821 ctgaggattc agactgacgc gaatcgccgg caacgaactg cctccgacgg tgacctcgcc
     2881 caccccttcc acttgcgaca gtttttgcgc tatgaccgtg gatgccagat cgtacaactg
     2941 cccttgccga accgtgtcgg aggtcaacgc cagcaccatg atcggcgccg aagccgggtt
     3001 caccttatga taggtggggt tgctgcgcaa actcgaaggc agcaagttgc gcgcggcatt
     3061 gattgccgcc tgcacatcgc gcgcggcgct atcgatgtcc cggctgaaat caaacgtcaa
     3121 cctgatgcgg gttgatcctt ctgtgctgct cgagctcatg tcggtcactc ccgcgatcga
     3181 gcccagcgat tgttccagcg gagtggcgac actcgacgcc atcgtctcgg ggctggcgcc
     3241 cgccatactg gcggaaaccg aaatgatggg aaaatcgacc tgcggcagcg gagccaccgg
     3301 taaaaaatga aacgccagca tgcccgtcag cacacacgcc gcacaaagca gcgtggtggt
     3361 gacaggccgc atgacgaata tcgtgaaaaa cctcatggca gcgcctgtgt gccaagcttg
     3421 ttttcgctgc ggccccggcg ggttcgccgc gacagattat cgaacatcag ataaatcacc
     3481 ggagtcgtga acaaggtcag gaactgactg cacaataatc ctccgaccat taccaggccc
     3541 aaaggctgac gcagttccgc gcccgtcccg gtggaaaaca taagaggcag cgcgctgaac
     3601 aatgccgcga gcgtcgtcat gagaataggt cgaaacctta acaaggccgc atcgtgaatc
     3661 gccgcctggg gcgacaagcc gcgatgccgc tcggcatcca gagcaaagtc gatcatcatg
     3721 atcgcattct tcttgacaat gccgatcagc aaaataatgc cgatcacgcc tatcatgtca
     3781 agctctgtat ggctgagcat cagggccagc aaggcgccga tcgcagcaga cggcagcgtc
     3841 gacaaaatgg tgaccggatg aatatagctc tcgtacaata tccccagcac gatgtacatg
     3901 gcggccacgg cggccagcaa taaccacaag gtgctcgaca gcgaggcctg aaaagcgcgc
     3961 gctgccccct ggtatttgga ctggatcgat atgggcatgc ccagagacgc ctgcgccctg
     4021 tcaatggcct ggaccgcgtc ggaaagcgcc acgccggggg ccaggttgaa cgagatcatt
     4081 gcggcgggaa actgatccac gcgctcgatc gacaacagcg tattggactg gacgagctgc
     4141 gccaggctgg tcagtggaac gggtgcgccc gaggtcgtcg gcacataaat ctgggacaac
     4201 gatgcggggg tttgccggta ctgcggcgcc acctcgagga cgacgcgata ttgcgtcgac
     4261 tgagtgaaaa tggttgaaat caaacgctga ccaaaagcgt catacaaggc ctcgtcaatc
     4321 gcggccgtgg tcacccccag acgagcggcc gcgtcgcgat cgatgttcaa ggtcgtttgc
     4381 aaaccgccgt tttgaaggtt gtcggcaaca tcgaccagtt gcggaagtgc ttgcagtttt
     4441 ttgacaacct gcggaaccca ttgaccaagc acgctctcgc tgggccccga taaactcatc
     4501 tggtactgag tgcggctgat ctgatcgtcg atggtcaagt cctgcacggg ctgcatatat
     4561 accttgattc cctgcaccga ctgcaaagct gttccgagac gatcaatgat ctgccgggag
     4621 tccgcgccac gctcgctgct cagtttcagc gcgatctgca tgcggccggt gttcagcgtc
     4681 gaattggtgc catcgatccc gataaatgac gacacgctcg acacatccgg atcttgcaaa
     4741 attttgtcca cagcctcttg ctggcgctgc gccatggctt gaaacgatac cgtttgcggc
     4801 gcttcgctta tggcctgaat gagccccgtg tcctgctgcg gaaaaaatcc tttgggcaca
     4861 agtacatata acaaacccgt gaaaagaaac gttcccagcg cgacccacag cgtcaggcgc
     4921 tgatgcgcca atacccaggt cagggcttga tcgtatgcat cgatcagcgc cttgatccag
     4981 gcccctgtct tgcgctgaaa acgccccagc ttcatctcgg attcgggcct gagcaaacgc
     5041 gcgcacatca ttggggtcag ggtaagcgag ataagcagcg acattaaaat ggcgacggcc
     5101 agcgtaatgg cgaattcacg aaatagccgt ccaatcacat cgctcatgaa caacagggga
     5161 ataagcacgg ctatcagcga cagtgtcagg gacaccagcg taaatccgat ttgcgcggcg
     5221 ccttttagcg ccgcctgcaa gggtctgtcg cccgcttcga tataccgcgc gatgttctcg
     5281 atcatgacaa tggcgtcgtc caccacaaat ccggttgcta tcgtcagcgc catcaacgtc
     5341 agattattga tactgaagcc gaccagatac atgatcccga acgtgcccac cagcgaaaga
     5401 gggacgacca cgctcggaat gaatgtggcc gtccagctac gcaggaaaac gaaggtcacc
     5461 atgaccacca gcgcaatcgc cagcaacagt tcaaactcca catgactgat cgagtcgcgg
     5521 atggtttgcg tgcgatccgt cgcgacggca atatcaaccc cgacgggcaa agactgtcgc
     5581 agcgaaggca aaagcgcttt gacgcggtca accacatcaa tcacattggc cgagggctgg
     5641 cgctgaatgt taagcagaac ggcgggcgtt cgtcctatcc atgccgcctg gcgaatgttt
     5701 tccgcgtcca gccgcgtact ggcgacatcg cccaggcgca gcggtgcgcc cttttcgtag
     5761 gtcagaatca gccggtcata gtcctgcacc gaactcaact gatcattcgc attaatcgtg
     5821 gtggcgcgtt gcggtccgtc cagatttccc ttgggctgat tgacgtttgc ggcgacgatg
     5881 gtcgtgcgca cgttcgacat ggtcagattg tgggcggcga gcgcctgcgg attgacctgc
     5941 acgcgcacag cgggacgctg gccgcctgca atgctcacca ggcctacgcc cgatatttgc
     6001 gacagttttt gcgcaacccg aacgtcgatc aggtcgcgga cctgatacaa gggcagcgta
     6061 ggcgaggtaa tcgccagact gatgaccggt gtatcggctg gattgacttt gttgtatagc
     6121 ggaggcgtag gcaagtcgga cggcaacaag ttggacgccg cattaattgc cgcctgaacc
     6181 tcttgttcgg ccacatccat ggccagatta agcccgaact gcaaggttat catcgacgaa
     6241 ccgcccgaac tcgatgacga catctgggtt aatcccggca tttgtccgaa ctggcgttcc
     6301 agcggcgagg taaccagcgc agtcatgacc tcgggacttg cgccggggta caaggtcacg
     6361 acctgaattg tcgggtaatc cacttcaggc aaggccgata cgggcaacag ccggtacgcc
     6421 agcaggccgg caatgaacaa tgccaccatc gcaagagtgg tggcaaccgg tcgaagaata
     6481 aacaggcggg acaagttcac ggaataggcc taatgcgccg aatggcccgc cggaacaggc
     6541 ggcgtggcag tcgcggaggc agcaggggca acgacgctga cggtcgcgcc ctcgcgcagg
     6601 cgatccagac cttcggtcac cacgcgctgg ccggcgctca aacccgactt gatcgcaaca
     6661 ttctggccgt ccacagtacc ggtcgtcacc ggctggacat gcaccttgtc atccgggttg
     6721 accagataca caaaaggccc gatcgaacct tgttgaacgg ccatcgtggg cgccagcagt
     6781 gccgcatcat tgctgctgac gtgcaaatgc acattgacaa attggttggg gaaaagcgat
     6841 tcatcttcgt tggcaaaacg cgctttgagc ttgaccgtgc cggtggccac gtcgatctga
     6901 ttgtcgatcg ccatcagttc acccgaggcg atcttgttgc gatcgtcgcg atcgtataaa
     6961 tcgaccgcaa gccgggtgcc tgccgccctt tgcgccagca cctcgggcaa ctgtgcttgc
     7021 ggcagagtaa atatcaccga aattggctgc gtctgcgtga tcacaaccag cccggaggtg
     7081 ctcgtggtcg aaatcaaatt gccctgatcg accatgcgca agcccaatcg ccctgaaatg
     7141 ggcgcggtaa ttcgtgtgaa atcaagctgc agtttcgcgc tgtcaacagc cgcctggtcg
     7201 cttttttctg tgccctggag ctgctgcacc agcgcggcct gtgcatcgac ctgctgtttg
     7261 gcaattgagt tctgcttgaa caacaactga taacgctgca agtcgcgccg agcgttctgc
     7321 aactgggcct gattctgttt ttgcgtgccc agtgcctgct caagctgcac ctggtaagtg
     7381 tcgggatcaa tctgcgccag aagatcgccc tgcctgacct tctggccatc gataaaagcg
     7441 actttccgca gcggaccggc gacgcggctt tgcaccgtga cggtattgaa cgcggtcacc
     7501 gttccgatgg ttttcagtgt cacgttgatg cggccattca ctacggtggc gattttcacc
     7561 ggcacggccg cggaagccat ggcgcttgca cgggccgagt tcgttgcacg ctgtttcacg
     7621 ctgtctttgc ggccaatcag ccagtatcca gcggccagca gaaccacaat aatgagaacc
     7681 gaagtccaga tgcggccgcg acgaggtcga ttaaattcgg cgcttgaatc cgacatgcac
     7741 agctctccta aggggaaata aagcaatgcc gctattctcg ctgatcagcg caagggcggg
     7801 cacggaaatt ccacgcctga aaccaaacgg aacaactaat tgtgccggct cagacgttgc
     7861 aacgtgcaag tttaaacgga atatcgcgcg tgacaggctg cggtttgagt tcctggtctt
     7921 gcgtggcaaa acgaacccat atcacatatt tgttggcgct catttcaggg aatacgccct
     7981 ggccagggtc gacccatacg cgcaacaact ggaacgtttt gccgctaagc atttcctggt
     8041 acgcgccctg cttggcttcc gcactgattt cgtcgctgga tttgcgcaac agacgcaaaa
     8101 tgagggcaag tccccggtac aaaggcaaaa aagggagtat ccactgctgc atgtcgtgca
     8161 gacgcgttgc caagggcttg atctgccagg cgtaatacga aggcatatca acctgggatg
     8221 ttccgcccgg caccgtaatg cgcccgcgca agctggccag ccattcgtta tcgcgcaaga
     8281 cctgccctat ccggccctgg gcggaaagtt cggtggacgc gctttgtatt tcctgcagca
     8341 tggcttcgag cgtgcgaaca tcgacactgg ggtgctggcg caatgcgctt agcgcgccgc
     8401 gctggcgctc cagatcttgc agcaccgctc cacgaagatc cgttcgatcg caaatgtcga
     8461 gcaggtcgaa taaagtcgcg accgttatct gctgaagatg gacatcttcg cctttggcga
     8521 aaaaaaacag ccggtcgaac aagtactcga ccctaagcag agcccggacg cgttcattgc
     8581 acggatattc gtaaagaacc acgtggccat aaaccttttt aagagccggc aagaactacc
     8641 ggtaagcttt gactaacgat accgcgtagc ctgggcaacc cagacatcat gcagcgcacg
     8701 ggcgcgttga gcgagttggc tgagcgtggt ttgcccatcg ttaagcagta cgtcgtcagc
     8761 tacagccagc cgggcctcgc gcgaggcttg cgttgatata atacgcgcaa tagcttcggg
     8821 cgtcagccca ctgcgcgcct gaacacgggc cctttgggtc tcgggatcgc aatcgacaac
     8881 gcaaatccga tccacccgtt cacgccagcg tcctgattca accagtagcg gaaccacaaa
     8941 aacaagataa caccctcgtg catgatccgc ctgacggtct gtctcgactt gtatcatagg
     9001 gtgaatgatc gcttcgagct gaccgcgcgc cgccgggttc gcaaacacca gttcgcgcat
     9061 gagttcacga tcgagcgagc catcgtcggc gatcatttcg ggaccgaaaa cgcgtcgtag
     9121 cgggcctatg gccggcccgc ccggccgggt aaggtcgtgg gcgatgacat cggtatcgac
     9181 gatcgccgca ccccaatctt taaaaaggcc ggcgacctgg ctttttccag accctatccc
     9241 tccggtcaac ccgattttat acatgtgccg tcgatcttca ttgccaaaag tagaaatgta
     9301 cctcagggcc ggccaataag acacccatgc cgcttatcgc caaaaacgga ccgaacggat
     9361 aggaagccga caaattcaag tttttctgac gcaggcatgc aaataaaact ccaagcaggc
     9421 acgccgccaa tagaacataa ggcaggcgtt ccagccccac ccacgctccc tgagcggcca
     9481 ggagcttgaa gtcgccctgc cccatcccct gacgattcct gatatgccaa taaagccaga
     9541 ataacagcca caagaagcaa tacccggcca tgacacccgc caccgagtcc tgcaaaaagg
     9601 cgggcccgcg cacccatgcg cacgccagac ccagccatag caagggaaat gtcaacgcgt
     9661 cgggcaacag catggtggcc gcgtccacca gggccaatac aagcataatc gccgacgcag
     9721 cgaccaaccc cgcaaaa
//
LOCUS       DATCXU010000425         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_911427,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000425 DATCXU010000000
VERSION     DATCXU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911427"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="flgF"
                     /locus_tag="VIR04_09565"
     CDS             complement(<1..533)
                     /gene="flgF"
                     /locus_tag="VIR04_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HEY9461038.1"
                     /translation="MDRIVYTAMSGAARVLEQQSVIANNMANVSTAGFREQLAVYRAV
                     PLVGDQGLPTRVTTVATTPGSNLRQGDMQETGRALDLAITGDGWFSVQTPTGEAYTRG
                     GELAVNAQGQLVTSQGLPVLSASGAPVAVPNRGTLTFSADGSVTALGAGDSPKDLQVI
                     GQLKLVNPPAASLERGG"
     gene            complement(545..1765)
                     /gene="flgE"
                     /locus_tag="VIR04_09570"
     CDS             complement(545..1765)
                     /gene="flgE"
                     /locus_tag="VIR04_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY9461039.1"
                     /translation="MSFGQSLSGLAAAAQDLDVIGNNIANSATVGFKSGAATFADVYA
                     NSRVGLGVQLASVNQNFAVGNITSTSNPYDMAIDGPIGMFRLQDPSGQVMYSRNGQFF
                     ADKDNFVVNTQGQRLTGYPVGTGTTNLVPLKVPVGNIAPSATTQMTSQVNLDANATVI
                     DPLVTPFDPTVSASYSYSLPITTYDSLGNAHQLTQYFAKAAGTATQSDWNIYYRLDSN
                     PLTSPTDAAPFAMSFNGAGTLISPVSSPLVVTQPGFANAPAAPLNINLDYTSSTQFGG
                     DFSSNFGQNGYTTGNFAGVSFSPEGAMVASYTNGKTQSVGSLALANFNNLQGLQSVGG
                     GAWVETSASGQAIVGRPGSNGLASIKGQSVEDSNVDISKELVKMIIAQRTYQANAQSI
                     KTQDQAMQSLISGI"
     gene            complement(1840..1992)
                     /locus_tag="VIR04_09575"
     CDS             complement(1840..1992)
                     /locus_tag="VIR04_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FLgD tudor-like domain-containing protein"
                     /protein_id="HEY9461040.1"
                     /translation="MAATDASGAPVSAQALTSGIVGSSLHASSGVKLDLGLAGTVALS
                     DVRKIM"
BASE COUNT          425 a          608 c          557 g          416 t
ORIGIN      
        1 tcgccgccac gctccaggct ggccgcgggc gggttcacca atttcaactg gccaatcacc
       61 tgcaggtctt tcgggctgtc acccgctccc aaagccgtta cggatccatc ggcggaaaac
      121 gtcagcgtgc cccgattggg aaccgccact ggcgccccgc tggccgatag cacaggcaag
      181 ccctgagacg tcacaagctg gccttgggca ttcaccgcca attcaccgcc gcgggtgtag
      241 gcctcgcccg tgggcgtctg cacgctaaac cagccatcgc cagtaatcgc caggtcgagc
      301 gcccgcccgg tttcctgcat gtctccctgg cgtaaattac tgcccggcgt cgtcgcaacc
      361 gttgtcacgc gggtcggcag gccctgatcg ccgaccagcg gaaccgcacg atataccgcc
      421 agttgctccc gaaagccggc tgtgctgaca ttagccatat tgttggcgat caccgactgc
      481 tgttcgagca cccgggccgc gccgctcatg gcggtataaa cgatgcgatc cataaagcgt
      541 cagcctagat tcccgagatc aggctctgca tcgcctgatc ctgggtcttg atgctttgcg
      601 cattagcctg ataagtgcgt tgcgcaataa tcattttgac caattccttg ctgatatcca
      661 cattggagtc ttccaccgat tggcccttga tggatgccag gccattggaa cccggacgac
      721 ccactatggc ctgacccgag gccgaggttt caacccaggc gccgccgcca acggactgca
      781 agccttgaag attattgaaa tttgccagcg ccagcgagcc caccgactga gtcttgccat
      841 tggtataact ggccaccata gcgccttcgg ggctaaacga cacccccgcg aagttgccgg
      901 tggtgtatcc gttttgaccg aaattggacg agaaatcccc accgaactga gtggagcttg
      961 tgtagtcaag attgatattc aacggggcgg cgggcgcatt ggcgaaaccc ggctgagtca
     1021 ccaccaaagg cgacgacact ggcgatatca gggttccggc gccgttgaaa gacatggcga
     1081 atggcgccgc atcggttggc gaagtaagtg ggttgctatc gagccggtaa taaatattcc
     1141 agtcgctctg cgtcgctgtc cccgcggctt tggcgaaata ttgggtcaac tgatgggcat
     1201 tgcccaaaga gtcgtacgtc gtaatcggca gagaatagct atagctggcc gaaacggttg
     1261 gatcaaaagg agtgacaaga ggatcgataa ccgtcgcatt ggcgtccaga ttgacctgac
     1321 tcgtcatctg ggtggttgcg ctcggggcga tattgcccac cggcactttc aaaggcacca
     1381 ggttagtggt tccggtgccg accgggtagc cggtcaggcg ctgcccctgc gtattgacta
     1441 cgaaattgtc tttgtcagca aaaaattgcc cgttgcgcga atacatgacc tgtccgctcg
     1501 gatcctgcaa acgaaacatg cctatggggc catcgattgc catatcgtac gggttgctcg
     1561 tgctggtaat gttgcctacc gcaaagttct gattgacgct ggccagttgc acccccagcc
     1621 cgacacgcga attggcatac acatcggcaa acgtggccgc gcccgattta aaacccaccg
     1681 tcgccgaatt ggcaatattg ttgcctatga cgtccaggtc ttgcgccgct gcggccaagc
     1741 cgctcaaact ttgtccgaaa ctcatgattg actatccttc gtaaattaag cgaactcgta
     1801 ccaaatggca gccgaaaact ctgccgtgaa caccgggcgc tacatgatct tccgtacatc
     1861 cgacaaggca actgtgccgg ccaggcccaa gtcgagcttc actccgctcg atgcatgcaa
     1921 ggagctgccc acgatgcccg atgtcagcgc ttgggcagag accggggctc cactggcatc
     1981 cgtggctgcc acctgaaccg tgtatg
//
LOCUS       DATCXU010000426         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_439323,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000426 DATCXU010000000
VERSION     DATCXU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439323"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VIR04_09580"
     CDS             <1..929
                     /locus_tag="VIR04_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY9461041.1"
                     /translation="AIAAGLSPLGVGADQGGSVRLPAHYCGIVGLKPTIGRIPMTGHM
                     PDTLHRYATAGVMARYVEDTRLALEVTSGADGTDWCAEVPDSLSESRQPLSSLRIGWI
                     AGDYFGTVDPEVASAVSSAATQLKGSVQSVTDLPQDFLPEFDCDLLSEGLYRAEGAQY
                     FEPILKGHHDLLHPWMLAELGRAARSLPDYLSAAAGVEQLRSALQGFFREYDILLCPV
                     APIVAPICGSDHIVVNGVERRLRSINRAVQPFNLCGVPAISVPYSMSTGGLPIGVQIV
                     GRKYQEQNVLNIAEFLETLRPGGRALPASALL"
     gene            complement(938..2152)
                     /locus_tag="VIR04_09585"
     CDS             complement(938..2152)
                     /locus_tag="VIR04_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY9461042.1"
                     /translation="MTPSSPSSNKPLSGIRVVDFSRFFAGPYCAQLLGDLGAEVIKVE
                     RPGEGDPLRVQGPPFHHNNGITFYATNRNKRSITLDLQSKQGQAVARDLCLKADVVLE
                     NFRPDVMPRLGLGYDELSAANPRLVYASISGFGADGPDMALGAFDLTIQAVGGYMSIT
                     GERGGAPVKLGTSGFDVLSGMNCQAAILASLLQRSVTGKGQKVETSLLETEVAFLVNA
                     AMEYLLTGVEPQKWGSEHAQQVPYKAFKTADGWLIIGAGFSNLYVKFCEVLGRKDLIS
                     DPRFDDMAGRVTNRIALYQILDAEIAKHDTASLSAKLDAVKVPCAPVNNMQQVFSHRQ
                     VKHRGMLQTLHHPSYGDVPSLGAAAKYSAFNVTDGWSAPPLLGENTDDILREWLAMSD
                     TQIAELRTAKVI"
     gene            complement(2155..2931)
                     /locus_tag="VIR04_09590"
     CDS             complement(2155..2931)
                     /locus_tag="VIR04_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9461043.1"
                     /translation="MALLLSELKGSALVLTLNRPEVLNALSSELSQEMLAAVQNANNR
                     RDVRTIIVTGAGDRAFSAGTDLKQRRTLSPDGKWAQSRDGWNTNQALMNSPKAVIAAI
                     SGWCLGGGFELALHCDLRIAADDARFGWPEMTLGAYPGGGAAIMLPRIIGRAKAKELF
                     FTARRITAAQALQLGLVERVVPRAELLDTALAYAADIETTSPLGLAAVKRSVNQGADL
                     SIQEAAELDQRIRRPLEATRDYEEGINAHFEKRKPVFRGE"
     gene            3107..>3696
                     /locus_tag="VIR04_09595"
     CDS             3107..>3696
                     /locus_tag="VIR04_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9461044.1"
                     /translation="MVKNKSTLREDSPSHGKLADKLYEELLGWIAHGPFGEGAKLPSE
                     EKLAIKYGVSRPVVREALSRLRLDGIIVSRHGSGSYVERRPKKEFFDFAPIGGVADLM
                     RCFEYRIALEGEAAGLAALRRSDADLKLIKSALNDLNRAIEQRVVGVDADIKFHNAIA
                     RATKNDLFVSAMATLADYTFAGMTVARNLSLRTSVSR"
BASE COUNT          772 a         1074 c         1041 g          809 t
ORIGIN      
        1 ccgcgatcgc cgctgggttg tcgccgctgg gggtgggcgc tgaccaaggc ggctcggttc
       61 gcttacccgc gcactactgc ggcatcgtcg ggcttaagcc gacaatcgga cgcatcccca
      121 tgacaggcca catgcccgac acgcttcacc gatatgcgac agcaggcgta atggcgcgat
      181 acgtcgaaga cacccgtctt gcgttggagg tgacaagcgg cgcggatgga acagactggt
      241 gtgccgaggt tcccgattcg ctcagcgaat ctcgtcagcc cttaagttcg ttgcgcatag
      301 gctggatcgc cggcgactat ttcggaacgg tcgatcccga ggttgccagc gcagtgagca
      361 gcgcggccac gcaattgaag gggtccgtcc agtctgtaac ggacctgccg caggatttcc
      421 ttcccgagtt cgactgcgat ttgttgtccg aagggcttta tcgcgcggaa ggcgctcagt
      481 atttcgagcc gattttaaaa ggacatcacg atctgttgca cccgtggatg ttggcggaat
      541 tagggcgcgc ggcgcgaagc cttcccgact atctttccgc ggcagccggg gtagaacagt
      601 tacgtagcgc gctgcaaggg ttcttcagag agtacgacat actgctgtgt ccggttgccc
      661 ctatcgttgc gccgatatgc gggagcgacc acatcgtcgt gaacggggtt gagcgccggt
      721 tgcgatcaat caatcgggcc gtgcagccat tcaatttgtg tggcgtcccg gctattagcg
      781 ttccgtactc catgtcgact ggtgggctgc ccattggagt ccagattgtc ggccggaaat
      841 accaggaaca aaacgtgctg aatattgcgg agttcctgga aacgctacgg ccgggcgggc
      901 gcgcactacc tgcaagtgcg ctactgtaat ccggggctta aatcaccttc gccgtgcgca
      961 gctcggcaat ttgcgtatcg ctcattgcca gccactcgcg aagaatatcg tcggtatttt
     1021 cgcccagcaa aggcggcgcg ctccagccat ctgttacgtt gaatgcggaa tacttagccg
     1081 cggcgcccag cgaaggcaca tcgccatatg aaggatgatg cagggtctgc aacatgcccc
     1141 gatgctttac ctggcgatgg ctgaaaacct gttgcatgtt attaaccggc gcgcacggca
     1201 ccttgaccgc atcaagtttg gcactaagac tggctgtgtc gtgcttggct atttccgcgt
     1261 ccaggatctg gtatagggcg atgcgattgg tgacccggcc ggccatgtcg tcaaaacgag
     1321 ggtccgaaat cagatctttg cggcccagca cctcgcaaaa cttgacgtac aggtttgaaa
     1381 acccggcgcc gataatcagc cagccgtcgg ctgttttgaa cgccttgtaa ggcacctgct
     1441 gggcgtgctc tgaaccccat ttctgcggtt cgaccccggt aagcaggtat tccatggccg
     1501 cgttgaccaa aaaggcgact tcagtttcaa gcagactggt ctcgaccttt tgtcctttgc
     1561 cggttacgga tcgctgcagc agcgaggcca gaattgcggc ctggcagttc atgcccgaaa
     1621 gcacgtcaaa gcccgacgtg cccagtttta caggcgcgcc gccccgctcg ccggtaatgc
     1681 tcatgtaacc gcccaccgcc tgaatcgtca ggtcgaacgc ccccagcgcc atatcgggac
     1741 cgtcggcgcc gaatcctgaa atggacgcat acaccagccg tgggttcgcc gccgataatt
     1801 cgtcgtagcc cagccctagc cgcggcatta catcgggcct gaaattctcc aggacgacgt
     1861 ccgccttaag gcaaaggtcg cgcgccaccg cttgcccttg cttgctctgc aaatcgagtg
     1921 tgatcgaacg tttgttgcgg ttcgttgcgt aaaacgttat tccgttgttg tgatgaaacg
     1981 gaggcccttg aacacgcaac gggtcgccct cgccaggcct ttctaccttg atgacttcgg
     2041 cgcccaggtc gcccagcaat tgcgcgcaat acggtcccgc gaaaaagcgg ctgaaatcga
     2101 ccacgcgaat tcccgagagg ggcttattgc tgcttggcga cgatggcgtc atgactactc
     2161 tccgcgaaat accggtttgc gtttttcaaa atgcgcattg atcccttctt cgtaatcgcg
     2221 cgttgcctcg agcggacgcc gtatgcgctg atcgagttcg gcggcttcct ggatcgataa
     2281 atcggcgccc tgattgaccg accgcttaac cgccgcaaga cccaatgggc ttgtggtttc
     2341 gatatcggcg gcatacgcga gcgctgtgtc cagcagttcg gcgcgcggca cgacacgctc
     2401 gaccaggccc agttgcaagg cttgcgccgc ggtaatgcgg cgcgcggtaa aaaacaattc
     2461 cttcgccttg gcgcgaccaa taatacgcgg cagcatgatc gccgcgccac cgcccgggta
     2521 ggcgcccaag gtcatttcag gccagccaaa acgagcgtcg tccgctgcaa ttcgtaaatc
     2581 gcaatgcagc gcaagttcga accccccgcc caggcaccag cctgaaatcg cggcaatgac
     2641 ggctttgggc gaattcatca gtgcctggtt cgtgttccag ccgtcccggc tttgcgccca
     2701 cttgccgtca ggcgacaagg tgcggcgctg tttaagatct gttccggcgc taaatgcccg
     2761 gtcgccggcg ccggtaacga tgatcgtccg cacgtcgcgc cggttattgg cattttgaac
     2821 cgcggccagc atttcctgag acagttcgct ggataacgcg ttcaatactt caggacggtt
     2881 caatgtcaga accaacgcac tgcctttgag ctcgctaagt aatagtgcca ctactttccc
     2941 cttgattcct gctgttgcct ttatggcttg acaaggctgg caaatttgtc aagttcgata
     3001 aaaacatgca agctatgatc tataatgtta ccgtagttct tttacaacta tagtatatgt
     3061 tttacaactt tgcaaacaag ctttatcaaa ctattgttga gcgaaaatgg tcaaaaacaa
     3121 aagcacgctt cgtgaagata gcccatcgca cggaaaactt gccgacaagc tatacgaaga
     3181 actgcttggc tggattgcgc atggcccgtt cggcgaaggc gccaaattgc cttctgaaga
     3241 aaagctggcc ataaaatacg gcgtgtcccg gcccgtggtg cgcgaggccc tgtccagact
     3301 tcgactggac gggatcattg tatctcggca cggctcgggc tcgtatgttg aacgtcggcc
     3361 caaaaaagaa ttcttcgatt tcgcccccat aggcggcgtc gctgatctta tgcgatgctt
     3421 cgaatatcgt atcgccctgg agggcgaggc tgcgggcctg gcggctttgc ggcgaagcga
     3481 cgccgatctt aaactaatca aaagcgcatt gaacgatttg aaccgcgcca tagaacagcg
     3541 tgtcgtcggc gtcgacgcgg acattaagtt tcacaacgca atcgcccgcg cgacaaaaaa
     3601 cgacttgttc gtatcggcca tggcaaccct tgccgactat actttcgcag gaatgacggt
     3661 cgcgcgcaat ctttccttaa gaactagcgt aagccg
//
LOCUS       DATCXU010000427         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_295422,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000427 DATCXU010000000
VERSION     DATCXU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295422"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VIR04_09600"
     CDS             complement(<1..192)
                     /locus_tag="VIR04_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HEY9461045.1"
                     /translation="MTQRFSRLFAIAMLIVSAGAMMSVSFDAQARRMGGGKSFGRQSS
                     NITQQRQAVTPPKAAAPAAT"
     gene            complement(383..1156)
                     /gene="ubiE"
                     /locus_tag="VIR04_09605"
     CDS             complement(383..1156)
                     /gene="ubiE"
                     /locus_tag="VIR04_09605"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY9461046.1"
                     /translation="MQNHPTDSDPSVSSGKTHFGYKTVAEAEKAHKVAEVFHSVANRY
                     DIMNDLMSMGLHRVWKAFTIGRADVRTGMKVLDIAGGTGDLALAFAKRAGPSGEVWLT
                     DINDSMLRVGRDRLMNKGLLVPTAVCDAEKLPFSDGYFDRVSVAFGLRNMTHKDRALS
                     EMRRVLKPGGKLLVLEFSRVAKPLAPAYDWYSFHILPWLGKKIAKDEDSYRYLAESIR
                     MHPDQETLAAMLGAAGLSRVQFFNLSAGVVALHEGVNLG"
     gene            1259..1948
                     /gene="phoB"
                     /locus_tag="VIR04_09610"
     CDS             1259..1948
                     /gene="phoB"
                     /locus_tag="VIR04_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY9461047.1"
                     /translation="MSTTILVIEDEPNIQELIAVNLSFAGYDVTRASSAEQAQQIIAA
                     ELPDLILLDWMLPGAQGIVLAKKLRAQERTRHVPIIMLTAKSAERDKLDGLEAGADDY
                     ITKPFSPKELVARIKAVLRRRSPQLTDDTVAVGTLVLDPASHRVTGRDTALNVGPTEF
                     RLLHFFMTHPNRVFSRAQLLDRVWGDHVFVEERTVDVHIRRLRKALQSSRHDAHIETV
                     RGAGYQFTART"
BASE COUNT          482 a          606 c          549 g          461 t
ORIGIN      
        1 ggtggccgca ggagccgccg cctttggagg cgtaacggcc tggcgctgct gcgttatatt
       61 ggacgactgc ctgccgaagc ttttgccccc ccccatgcgc ctggcctggg cgtcgaaaga
      121 taccgacatc atcgcgcccg ccgatacaat cagcatggcg atggcaaata gccgtgaaaa
      181 ccgttgcgtc atttaaaact cctgtggcgc tgcgctaaac aaaccaggcc aatatgttga
      241 tatcatgaaa tatttagttc caggaacaag aatacaccag aaactgactg gaaactgaac
      301 attatcatat aaacggtgcg cgtgtctgtg tgtgggtgtc ttggctacaa cacctcgccg
      361 ggccgtctgc cggatttgcg atttacccca gattcacgcc ttcgtgcaac gccacgacgc
      421 cggcgcttag attgaaaaac tgcacgcgcg agaggccggc cgcgcctagc atggccgcca
      481 gagtttcctg atcgggatgc atgcggatgg attccgccag ataccgatag ctatcttcat
      541 cttttgctat ttttttccca agccatggca aaatatgaaa ggaataccag tcataagcag
      601 gcgccaacgg ttttgccacg cgtgaaaatt caagcaccag aagtttgccg cccggtttca
      661 acacgcgcct catttcggac agcgcacgat ctttatgtgt catgttgcgc aagccgaagg
      721 ccacgctgac ccggtcgaaa tagccatctg aaaagggcaa tttttcggcg tcgcagaccg
      781 ccgttggaac cagcaggcct ttgttcatta agcgatcgcg cccgacacgc aacatcgagt
      841 catttatatc ggtaagccag acctcgcccg acggcccggc gcgtttggcg aaggctagcg
      901 ccaaatcgcc ggtgccaccc gcgatatcca gcactttcat gcccgtgcgc acatcggcgc
      961 gtccaatcgt gaaagccttc catacgcgat gcaaccccat cgacatcaaa tcattcataa
     1021 tgtcgtagcg attggccacc gaatgaaaga cttccgccac cttgtgcgct ttttcggctt
     1081 cagccaccgt tttgtagccg aaatgggtct tgccggagga aacagaagga tcggaatccg
     1141 ttgggtgatt ttgcatagtt aatgacaatc ttgccgcgct tttcataatt ctgaaacatt
     1201 aacgacatag tatcgcgtct gccttgacca aaccactcta aaccattctt cgactctaat
     1261 gagcacaact attctcgtca tcgaagacga gccgaacatc caggaactga ttgcggtcaa
     1321 tctgagcttt gcaggctatg acgttacgcg ggcgtcaagc gccgagcagg cgcaacaaat
     1381 aatcgcagcg gaattgcccg atctgatttt gctggactgg atgctgcccg gtgcgcaggg
     1441 gatcgtcctg gcaaaaaagc tgcgcgccca ggaacgaacc cggcatgtgc ccattatcat
     1501 gcttacagcc aaaagcgccg agcgcgacaa gctcgatggc cttgaagccg gcgcggacga
     1561 ctacatcacc aaaccatttt cacccaaaga gcttgtcgcc cgaatcaaag cggtattgcg
     1621 ccgaaggtct ccgcaactga ccgatgacac cgtcgcggtg ggcaccctgg ttctggaccc
     1681 ggccagccat cgtgtcacgg gccgcgacac ggcgctgaat gtcgggccca ctgaatttcg
     1741 cctgctgcac tttttcatga cccatccgaa tcgcgttttt tcccgcgccc aattactgga
     1801 tcgggtttgg ggcgaccatg tttttgtgga agaacgcacc gtcgatgtgc atattcggcg
     1861 actgcgcaag gcgctccaat caagccggca cgacgctcac atcgaaacgg tccgcggcgc
     1921 gggataccag ttcacagcca gaacctgaga caatatccag atggttaaaa ccttattgtc
     1981 aatggccctg tggggggcgc tggggtggct tttcagcttg tggatcgaca cacggctggg
     2041 ctggtcaatc ttttcgctgg gactcatcgc catgattctg gtcagtggtg tccagctt
//
LOCUS       DATCXU010000428         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_417121,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000428 DATCXU010000000
VERSION     DATCXU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417121"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VIR04_09615"
     CDS             complement(<1..495)
                     /locus_tag="VIR04_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY9461048.1"
                     /translation="MPISLLSSTQAQSVSVAAAGSRRHRRSHSNQQEGSRPPAALHGL
                     LLATALGLAALWMGRWAPIVGGPVFGIVLGMIIRNSVGVGPIFRPGLKIASRQVLQWS
                     IIALGFGLSIRQVAKTGMESLSVTAVTICLAFGSAYLLGKWLNIPSKLKILIGVGTAI
                     CGGSA"
     gene            592..1512
                     /locus_tag="VIR04_09620"
     CDS             592..1512
                     /locus_tag="VIR04_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461049.1"
                     /translation="MIGISVRQLEVFVSIAATARVRLAAERLSVTQPAVSMALAELER
                     QLNTPLFDRARGRLRLNSRGKELLPMAQEIIERLHEMRRHINNDPMTLSGELRVGTSN
                     TVGNYLIGDLLKPFINAYPNVALKVSVANTHTIVDDLLSHRIDAGCVEGPVNHSQIEA
                     LAWRNDELVVCAAPGHPLARLKRLRAGHFANAKWILREPGSATRALTERALTSLPPGQ
                     IVLELGQVEAIKQAVIAGLGIACLPIAATTDSAAAGRLKVLNTPFLDLHRRLSLVLHK
                     ARYRGALIDAFVASLDCGSARPSADQSSAD"
     gene            1575..2807
                     /locus_tag="VIR04_09625"
     CDS             1575..2807
                     /locus_tag="VIR04_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY9461050.1"
                     /translation="MARSSNKAGATASATQVKSGGTQFDLPPYEVVALVLQGGGALGA
                     YQAGVYQGLTEAGIKLNYLSGISIGALNIAIIAGNAPGRRIERLTEFWETICEPNFGC
                     PVNAFAEQSFFDLNDATRRSMNALSAASAIVNGQKGFFSPRFPPASSWVSGSPGAASY
                     YDTSALKDTLERLCDFDRINSTQQHVSVGAVHVSTGNLVYFDNTRTKLQAEHFMASGA
                     LPPAFPAVEINGEYYWDGGLVSNTPLSHVLESRPFKDTLAFQVDLWSARGRVPTNMSE
                     VSDRMKAIQYSSRTRMITDQVRLMQNMRHLLQHMQESLPPEKRGKGPYSKIAEDLACG
                     KRYNVVHLIYHGQPYEKQYRQGRYRLASMRNRWASGLKDIRDTLSDPARLSMPDSNTG
                     FVTHDVHRDRIDESMKAD"
     gene            complement(2822..3748)
                     /locus_tag="VIR04_09630"
     CDS             complement(2822..3748)
                     /locus_tag="VIR04_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9461051.1"
                     /translation="MTSVPLISQQTRSKEFWIGFFLAGLGAVLFSAKAIVVKLTYHYD
                     VDPLTVIGFRMMMSLPFFAAIGWFQARKARRAELPLLTRKEGLQIIFLGFIGYYLASL
                     LDFYGLQTISAGLERLILFLAPTFVLLFTALYFRRPIAGRQWAALAVSYVGVVLVFLQ
                     DLSFSGSQVLVGAGFVLASALSYAFYLIGSGELIKRVGATRLVAYAMSVSSIVTLVHF
                     FGVHSWQGLIQPLPVYQLSLFHAIVNTVIPVFLIMWSVARIGAPMTAQLGLLGPVSVL
                     FLANWILGEPITSLQLLGTAFALAGAVLLAHR"
BASE COUNT         1040 a         1182 c         1053 g          768 t
ORIGIN      
        1 ggccgacccg ccgcagatag ccgtgcccac gccgatcaaa atctttaatt tcgacggtat
       61 attcagccat ttcccaagca aatacgcgga accgaaagca aggcaaatcg taacggccgt
      121 caccgataat gattccattc ctgtcttggc gacctgcctg atgcttaaac caaagcccaa
      181 cgcaataatc gaccattgca atacttgacg ggacgcgatc ttaaggccgg gtctgaaaat
      241 cggacccaca cccaccgaat tgcgaataat cattcccaac acaatcccga atacgggacc
      301 gcccacaatg ggcgcccagc gccccatcca caatgccgcc aatcccagcg ctgtggccaa
      361 tagcaaacca tgcaaggcgg cgggcgggcg cgatccctcc tgttgattcg aatggctgcg
      421 acggtggcgg cgcgaaccgg ccgccgcaac cgagacggac tgagcctggg tcgaggataa
      481 aagtgatatt ggcacgattg atcgttatat gttaatgaat ttcagtaata ttaaacgtat
      541 gtcgtgtata aaaaaattca ttaatattta tctatcctat aaaatggatt gatgattgga
      601 atcagtgtcc gccagcttga agtcttcgtg tcgatcgccg cgaccgcgcg cgtgcggctg
      661 gcagcagagc gcctgtccgt cacacaacca gcggtcagca tggcgctggc cgagctcgag
      721 cggcagctta atacgccact ttttgatcgg gcacgaggcc gcctgcgttt aaactcgcgc
      781 ggtaaagagc tccttcccat ggcacaagag atcatcgagc gccttcacga aatgcggcgc
      841 catataaaca acgatcccat gacgctaagc ggcgaattgc gcgtgggcac cagcaacact
      901 gtcggcaatt acctgatcgg cgacttgctc aaacccttta tcaatgcata cccgaacgta
      961 gcgcttaaag tcagcgttgc aaacactcac accattgtgg atgacctgtt atcgcaccgt
     1021 attgatgcag gctgcgtcga aggcccggta aaccattcgc aaatcgaagc gcttgcctgg
     1081 cgcaacgacg agctggtggt gtgcgcggcg cccggccacc cgttggcgcg acttaaacga
     1141 ctgcgtgccg ggcacttcgc aaatgcgaaa tggatcttgc gtgaacccgg ttcagccact
     1201 cgcgcattga ctgaacgcgc cttgacttca ttgccgccgg gccagatcgt gctcgagctg
     1261 ggccaggtcg aagccattaa acaagccgtt atcgccgggc tgggaatcgc ctgtctgccc
     1321 attgccgcca ccaccgattc cgctgccgcc ggccgcctga aggttttgaa cacccccttt
     1381 ctggacctgc atcggcgctt gtcgctggtg ctgcacaaag cgcgttatcg cggcgcatta
     1441 atcgacgcat ttgtggccag cctggattgc ggaagcgcca ggccctctgc ggatcagtcc
     1501 agcgcggact gatccgccgt agcggcgcag tcgattacac tgaagctaac tatttccgga
     1561 taccctcaaa ttcaatggca agatcttcta ataaagcggg cgctacagcg tcggcaaccc
     1621 aagtcaaatc cggcggcacg caattcgatc tgcctccata cgaagtcgtg gcgctggtat
     1681 tgcaaggcgg cggagcgctg ggcgcctatc aggcaggcgt ctaccagggc ctgaccgaag
     1741 cgggcatcaa gcttaattat ttatcgggca tctcgatcgg cgcgctgaat attgcgatca
     1801 ttgcgggcaa tgcgcccgga aggcggattg aacgcctgac ggaattctgg gaaaccattt
     1861 gcgagccgaa tttcggctgt cctgtcaatg cttttgcaga gcaaagcttt ttcgacctga
     1921 acgacgcgac ccggcgatcc atgaacgcgc taagcgctgc aagcgcaatc gtcaacgggc
     1981 aaaaaggttt tttcagccca aggtttccgc ccgcttcatc atgggtatcg ggctcgcccg
     2041 gcgccgccag ctattatgac accagcgcgt taaaggacac gcttgagcgc ctgtgcgact
     2101 ttgatcgcat caactcgacc caacagcatg tctcggtcgg cgccgtacac gtcagcacgg
     2161 gcaatctggt gtactttgac aacacccgga caaagctgca agccgaacat ttcatggcct
     2221 ccggcgcgtt gccgccggcc ttccccgccg tggaaatcaa tggcgaatat tattgggatg
     2281 gcggtctggt ttcgaacacg ccgctttcgc atgtgctgga atcgcgccca ttcaaagaca
     2341 cgctggcatt tcaggtggat ctgtggagcg cgcgcggccg cgtaccgacc aacatgagcg
     2401 aagtgtcgga caggatgaaa gccattcaat actccagccg aacacgcatg atcaccgacc
     2461 aggttcgctt aatgcaaaac atgcgccacc tattgcagca tatgcaagaa agcctgccgc
     2521 ccgagaagcg cggcaagggg ccttacagca agattgccga agaccttgct tgcggcaaac
     2581 gctataacgt cgttcatctg atttatcacg gccaaccgta cgagaaacag taccggcaag
     2641 gtcgataccg tctggccagc atgcgcaacc gctgggccag tggcctgaaa gacatccggg
     2701 atacgctatc ggatcccgcg cggctttcca tgcccgacag caataccgga ttcgtcacac
     2761 acgacgtgca ccgcgatcgg atcgatgaat cgatgaaagc cgattaggtc aaatcagccc
     2821 gtcaacggtg cgccaatagc accgcacctg ccagcgcaaa tgctgtgccc agcagttgca
     2881 acgacgtaat cggctcgccc aggatccaat ttgccaaaaa caatacagaa acaggcccca
     2941 gcaagcctag ttgcgccgtc atgggtgcgc cgatacgggc caccgaccac atgatcaaaa
     3001 acaccggaat caccgtattg acgatggcat gaaacagaga taactgataa accggcaaag
     3061 gctgaatcaa gccctgccag gaatgcacgc caaaaaaatg gaccaaggtc acaatggaag
     3121 agaccgacat ggcgtaggcc accagacgcg tggctccgac gcgtttaata agctcgccgg
     3181 agcctattag gtaaaacgca taggacaagg cggacgccag cacgaaaccg gcccccacca
     3241 acacctgact accgctaaac gacaagtcct gcagaaagac cagcacgacg ccgacatagg
     3301 aaactgccag cgcggcccac tgcctgccgg cgatcggccg tctgaaataa agcgccgtaa
     3361 aaagcaatac aaacgtcggc gccagaaaca aaatcaggcg ttcgaggccg gccgaaatgg
     3421 tttgcaaccc atagaagtcc agcaagcttg ccaaataata gccaatgaac cccagaaaga
     3481 taatctgcag accctctttg cgggtcaaaa gcggcaattc ggcgcgccgc gccttgcgcg
     3541 cctgaaacca ccctatcgcc gcgaaaaagg gcaaggacat catcatgcga aagccaatga
     3601 ctgttagtgg atcaacgtca taatggtaag taagcttgac aacaattgcc ttggccgaaa
     3661 ataaaaccgc acccaggccg gccagaaaga agccgatcca gaattctttc gatcgtgttt
     3721 gttgcgaaat taagggtacg gatgtcatgc cgccgaatct tgataaagga tggagacaca
     3781 gtataaggac caggctccgc atgcgaaatc aaagtgcaat gcggcgttgt tattttgcct
     3841 aaaattgtct gcgtgattaa atagtgctta ggctgtagtt gcctaggcaa taatatgctc
     3901 ctataattat tttcatgatc aaacctgaac actcttcgac cgccaagatc atgtcttctt
     3961 ccgtcacagc aagcggccac gcgcgcaaac ccgccaccaa aaagaaagcg gccggggaaa
     4021 acgcggtcca caaaaccgac gct
//
LOCUS       DATCXU010000429         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_282594,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000429 DATCXU010000000
VERSION     DATCXU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282594"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VIR04_09635"
     CDS             complement(<1..729)
                     /locus_tag="VIR04_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY9461052.1"
                     /translation="MAHSSAKHHTIAALVLLGVVAAVCVSLGNWQLRRAAQRDTIKLA
                     IEDGRSHPPLQLNAHTPHKELINWRPAVVSGTWLDKFTVLLENRNFKGMPGFWVATPL
                     LIAPDTAVLVLRGWVARPLPPADMQPSIPAPKSGVAIEGELLNRVPRLFELWSFGKRT
                     EGQLPAKLPTANLVAPKVQNLDLADYAKASGLTLLPSILEQTSDDHDQLGREWPQPSI
                     DADTNRGYALQWFSFAAVAAIAWLA"
     gene            765..974
                     /locus_tag="VIR04_09640"
     CDS             765..974
                     /locus_tag="VIR04_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY9461053.1"
                     /translation="MQIVVIVAFLGIVGSLVSALVYLMRDRGKTDRTVNALTVRVGLS
                     IALFLFILFAHYMGWIQATGIVQPQ"
     gene            complement(1036..1911)
                     /locus_tag="VIR04_09645"
     CDS             complement(1036..1911)
                     /locus_tag="VIR04_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY9461054.1"
                     /translation="MSAGHSALTKEAPYYYVPPMSPHPVRASLSFLAIMIGAAFWINH
                     YDAGFWLFILGVASLATVLFKWFGDAIRESEHGQNSKRVDFSYRWGMTWFIFSEVIFF
                     ACFFGALWYTRDVTLPLLNDLDHQALLWPHFTSAWPNFGPAGTVAPFESVGPFWLPTI
                     NTALLLSSAVTLTIAHHALRASHRGKTIFWMIVTVVLGFAFVACQAYEYHHAYTELNL
                     RFDSGAFGSLFYMLTGFHGFHVIMGATMLTVILIRLIKGHFTADHHFGFEGAAWYWHF
                     VDVVWLGLYIFVYWL"
     gene            complement(1947..2138)
                     /locus_tag="VIR04_09650"
     CDS             complement(1947..2138)
                     /locus_tag="VIR04_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEY9461055.1"
                     /translation="MRSFLKTLKAVAWGFFGVRKNKDHAQDTQTIDPFHLIIAGLLAG
                     AVLVIGLLLIIRWVVPGLS"
     gene            complement(2138..2239)
                     /locus_tag="VIR04_09655"
     CDS             complement(2138..2239)
                     /locus_tag="VIR04_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEY9461056.1"
                     /translation="MTPEQRRRNRNTALILAAVVVAIFAWSLYEGIT"
     gene            complement(2248..>3112)
                     /locus_tag="VIR04_09660"
     CDS             complement(2248..>3112)
                     /locus_tag="VIR04_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY9461057.1"
                     /translation="DPVIYQHVFWFFGHPEVYIMILPAFGIISHIVPTFARKKLFGYS
                     SMVYALASIAILSFLVWAHHMFAVGMPLTAQLFFMYGTMLISIPTGVKVFNWTATMWR
                     GSMTFETPMLFAIGFIFVFTMGGFTGLVVAIAPIDIQVTDTYYVVAHFHYVLVAGALF
                     AMFGAAYFWLPKWTGRMYDERLGKWHFWNSMLSFNLAFFPMHFLGLAGMPRRYADYAS
                     QFTDFHQIATIGAFWFGLSQIIFMYVVMKCYAGRGAVAPANPWEGAEGLEWVVASPAP
                     HHTFVTPPVIN"
BASE COUNT          761 a          822 c          929 g          600 t
ORIGIN      
        1 ggccagccaa gcgatggcag ccaccgcggc gaatgaaaac cattgcaagg cgtagcccct
       61 gttggtgtcg gcgtcaatcg acggctgcgg ccattccctg cccaattggt catgatcgtc
      121 cgatgtttgc tccaggatcg atggcagcag cgtcaggccg cttgcctttg catagtccgc
      181 aagatccagg ttctgcacct tgggtgcgac aaggtttgcg gttggcagct ttgcggggag
      241 ctgtccctcg gtccgcttgc cgaaggacca tagctcaaac agccggggca cgcggtttaa
      301 aagttcgcct tcgatggcga cgccactttt cggggcggga atcgacggct gcatgtcggc
      361 gggcggcagc ggacgcgcga cccagccccg tagcaccagt actgcggtat cgggtgcgat
      421 cagcaggggc gtggccaccc agaagccggg cattcccttg aaattgcggt tctccaggag
      481 caccgtgaat ttgtcaagcc atgtgccgct gaccacggcc ggccgccaat taatcaattc
      541 cttgtgcggc gtgtgggcat ttaactgaag cggcgggtgg ctacgcccgt cctcaatagc
      601 cagtttgatg gtgtcgcgtt gcgccgcgcg acgaagctgc cagtttccca aagagacgca
      661 cacggccgcc acgacgccca ataaaaccag ggcggcgatg gtgtgatgct ttgcgcttga
      721 atgggccatg tctgtaaaat agtgctgtcg ctggagactg aaagatgcaa atcgtcgtta
      781 ttgtcgcgtt tctgggtatt gtcggaagcc tggtgtccgc cctggtttat ttgatgcgcg
      841 accgtggtaa aaccgatcgt actgtcaatg ccttgaccgt gcgggtaggc ttgtcgatcg
      901 cgctgttttt atttatatta tttgcccact acatgggctg gattcaggct actggcatcg
      961 tgcagcccca gtagttggga aaaggggccg ccaaagccgc cccttttttt gtgtgcgatt
     1021 gcgacaggcg ggcggttaaa gccaataaac gaaaatatac aaccccagcc aaacgacgtc
     1081 cacgaagtgc cagtaccacg cggcgccttc aaaaccaaag tgatgatcgg cggtgaagtg
     1141 ccccttgatc aggcgaatca gtatgaccgt gagcattgtg gcgcccatga tcacgtgaaa
     1201 gccgtgaaag ccggtcagca tgtagaacag cgagccaaag gcccccgagt cgaaacgcag
     1261 gttcaattcg gtgtaggcgt gatggtattc ataggcctgg caggccacga acgcaaaacc
     1321 cagcacaacc gtgacgatca tccagaatat agttttgccg cgatggctgg cgcgcagcgc
     1381 atggtgcgcg atggtcaggg tcacggccga tgacagcagc aaagcggtgt tgatcgtggg
     1441 aagccagaat gggccaaccg actcgaaagg cgcgaccgtg ccagccgggc cgaaattggg
     1501 ccatgcgctg gtgaaatgag gccatagcag cgcctggtgg tcaaggtcgt tgagcagcgg
     1561 caacgtcacg tcgcgcgtgt accagagcgc gccgaaaaaa caggcaaaaa agatgacttc
     1621 cgaaaaaatg aaccaggtca tgccccagcg gtacgagaaa tcgacgcgct tgctgttttg
     1681 gccatgctcg gattcgcgga tggcgtcgcc gaaccatttg aacagaactg tggcgagcga
     1741 cgccacgcca aggatgaaaa gccaaaagcc cgcgtcataa tggttgatcc agaacgctgc
     1801 gccgatcatg atcgcaagga aagacaaact tgcgcgcacc ggatgaggcg acatcggcgg
     1861 tacgtagtag taaggcgctt ctttggtaag cgccgagtga cctgcactca tggtgtactc
     1921 ctgatacgaa tttattgtat aaaaaatcag cttagaccgg gcacgaccca gcggatgatc
     1981 aaaagcagtc cgataaccag caccgcgcct gcgagcagac cggcaataat aagatgaaac
     2041 gggtcaatcg tttgcgtatc ctgcgcatgg tccttgtttt ttcgtacgcc gaaaaaaccc
     2101 caggcaacgg ctttaagcgt tttgaggaaa ctgcgcatta cgttatgccc tcgtacaggc
     2161 tccaggcaaa gatcgcgacg acaaccgcag ccaaaattaa agcggtgttg cggttacgtc
     2221 gacgttgttc cggtgtcata gtaacgctca attgatgaca gggggtgtaa caaaggtgtg
     2281 gtgaggcgcc ggcgaagcca caacccattc aagaccttcc gcgccttccc atgggttggc
     2341 gggggcgacg gcgccgcgtc cggcatagca cttcatgacg acgtacatga agatgatttg
     2401 cgacaaaccg aaccagaatg cgccaatggt ggcgatttgg tgaaaatcgg taaattgcga
     2461 agcatagtcg gcataacggc ggggcatccc ggccagaccc agaaagtgca tggggaaaaa
     2521 tgccagattg aacgaaagca tgctattcca gaagtgccat tttccaagcc gttcgtcgta
     2581 catgcggccg gtccacttgg gcagccagaa gtaggcggcg ccaaacatcg cgaataaagc
     2641 gcccgcgacc agaacgtagt ggaagtgcgc cacaacgtag taggtatcgg tcacctgaat
     2701 gtcgatgggg gcgatggcca cgaccagacc ggtaaatccg cccatcgtga acacgaagat
     2761 aaagccgatg gcaaatagca tgggcgtttc aaatgtcatt gagccgcgcc acatggtcgc
     2821 cgtccagttg aacaccttaa cgcctgtcgg aatcgagatc agcattgttc cgtacatgaa
     2881 gaacaattga gcggtaagcg gcatgcccac ggcaaacatg tggtgagccc agaccaggaa
     2941 cgacaggatg gcgatcgacg ccagcgcgta aaccatggat gaatagccga acagcttttt
     3001 gcgcgcgaaa gtgggcacaa tgtgcgaaat gattccgaac gccggcagaa tcatgatgta
     3061 aacctcgggg tgaccgaaga accagaacac gtgctgatag atgacgggat cg
//
LOCUS       DATCXU010000430         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_488161,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000430 DATCXU010000000
VERSION     DATCXU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488161"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIR04_09665"
     CDS             <1..273
                     /locus_tag="VIR04_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY9461058.1"
                     /translation="VAIKPEVLLLDEPCSALDPISTAKIEELIAELKSDYTVVIVTHN
                     MQQAARCSDYTAYMYLGELMEFGQTDQIFVKPVRKETEDYITGRFG"
     gene            complement(274..771)
                     /locus_tag="VIR04_09670"
     CDS             complement(274..771)
                     /locus_tag="VIR04_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY9461059.1"
                     /translation="MSENPDKKDWRSEGVRVVHADQLDVNTAQTPGMNRAAAINYARV
                     GAQKIWAGTVTIHPKAKTGAHHHGALESVIYIVRGKARMRWGDRLEFVAEAGPGDFIY
                     VPPYVPHQEMNASDEEPLECVLVRSDNEAVVINLEIAAAENPEEVYWVDPIHKDPHPH
                     SHPHK"
     gene            complement(834..965)
                     /locus_tag="VIR04_09675"
     CDS             complement(834..965)
                     /locus_tag="VIR04_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461060.1"
                     /translation="MSAQKQAQSFRNFKVEPLDWVAPDLEESDNDTGNEKPAQEANS"
     gene            1197..2384
                     /locus_tag="VIR04_09680"
     CDS             1197..2384
                     /locus_tag="VIR04_09680"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY9461061.1"
                     /translation="MSDGSVLVINTGSSSLKFGLYAERNRDELLLFGGVAQGIGGDGG
                     KLEFKDAHGRVLLSQSIRLASQADALDRATHWLDQVSDGKPCAIGHRVVHGGPRLSNH
                     QRVTPALLAELGACLHFAPLHIPIALKLMESAARTYPGIPQFACFDTVFHNTMPDKAV
                     RFALPGKLFDEGIRRYGFHGLSYESIVDQFGTTLPGRTVIAHLGNGASLVAVKDGRSV
                     DTTMGLTPTGGIPMATRSGDLDPGVLLYLLRVKRMGADALEELLNHDSGLTALSGGRA
                     DMRDLQAAANNGDGQARLAIETFCSAISKVVAGYAAALGGLDLLVFTGGIGEHSARVR
                     KDVCQDLKFLGVSIDDSENQLHRGTISTTQSGVRVRVVSSQEGRQIARHCRAMMRSPV
                     SGV"
     gene            2388..>2555
                     /locus_tag="VIR04_09685"
     CDS             2388..>2555
                     /locus_tag="VIR04_09685"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461062.1"
                     /translation="MTIAQNAERQVAGSAQPLAPDDLRKIDAYWRACNYLCAGMIYLR
                     DNPLLKEPLRPA"
BASE COUNT          489 a          734 c          745 g          587 t
ORIGIN      
        1 gttgccatca agcccgaagt gctgttgctt gacgagccgt gttccgcact tgacccgata
       61 tcgaccgcca aaatcgaaga actgattgcc gagcttaagt ccgactacac cgtagtcatc
      121 gtcacccaca atatgcagca agcagcgcgc tgttcggatt acaccgcata catgtatttg
      181 ggcgagttaa tggagttcgg ccaaaccgac cagatctttg tcaagccggt gcgtaaagag
      241 accgaggact atattaccgg gcggtttggc taatcatttg tgcgggtgcg aatgtgggtg
      301 cgggtctttg tggatggggt cgacccagta cacttcctcg ggattttcgg cggcggcgat
      361 ctccagatta atgacgaccg cttcgttgtc gctgcgcacc agcacgcatt ccagcggctc
      421 ttcgtcgctg gcgttcattt cctgatgcgg cacataaggc ggcacgtaga tgaaatcgcc
      481 aggacccgct tcggctacga attccagacg gtcgccccaa cgcatgcgag ctttcccgcg
      541 gacgatgtaa ataacgcttt ccagtgcgcc gtgatggtgc gccccggttt tggccttggg
      601 gtggattgtg acggtgcccg cccaaatctt ttgagcgccg acgcgcgcgt agttaatggc
      661 tgccgcgcga ttcattccgg gcgtttgcgc cgtgttaacg tcgagctgat cggcgtgcac
      721 cacgcgcacc ccctcgcttc gccaatcttt cttgtctgga ttttcgctca tgttgccccc
      781 aaattagatc ggattatcat acgtcgggaa cgcgaaaaaa gccacggccg cccttaactg
      841 ttggcctctt gcgccggctt ttcattgccg gtgtcgttgt cggattcttc aaggtcgggg
      901 gcgacccagt caagtggttc gactttgaaa tttctaaatg attgagcttg cttttgagcg
      961 gacatgcgac gtactcccta acgtaacgcg gcggcgcgca aggcatcgcc gctatcaatg
     1021 gtcattgtaa catatttgcc cggtccttat cacgcaactc attgattgtg caggaattat
     1081 ggtgttgtta ccgcattcac cgagatcaag ttcgacctcg gacgccagcg tcgtgttaag
     1141 ctgaatcgtc tgccggacaa ggaaggcgcc ggcattacaa aaagcgagtc cgcacaatgt
     1201 cagacggatc ggttcttgtc attaatacgg gatcctcgtc gctgaagttc ggcctgtacg
     1261 ccgagcgcaa tcgcgacgag ctgcttttgt tcggtggggt ggctcagggc attggcggcg
     1321 atggcggcaa gctcgagttc aaagacgcgc atggccgcgt gctgctatcc cagtctatcc
     1381 gccttgcatc gcaggcggac gcgctggatc gcgccacaca ctggctcgac caggtttctg
     1441 atggcaagcc gtgcgcgatc ggccaccgtg tcgtgcacgg cggacctcgc ctgagcaatc
     1501 atcagcgggt tacgcccgcc ttgctcgccg aactgggcgc ttgcctgcac ttcgctccgt
     1561 tgcacatccc cattgcactg aaactcatgg aaagcgccgc ccggacttat ccgggcatcc
     1621 cgcagtttgc ctgcttcgac acggtttttc ataacacgat gccagacaag gccgtcaggt
     1681 ttgcgcttcc cggaaagttg tttgatgaag gaattcgccg ttatggcttc cacgggcttt
     1741 cttatgaatc catcgtcgat caatttggca caactcttcc gggccgcacg gtcatcgcgc
     1801 atctgggcaa cggcgccagc cttgtggcgg taaaggacgg gcgatcggtt gataccacga
     1861 tgggtttgac gcccaccggc gggataccga tggccacccg aagcggcgac ctggatccgg
     1921 gagtgttgct gtaccttctg cgggttaaac gcatgggcgc tgatgcgctc gaagaattgc
     1981 tcaatcacga ctcgggcttg acggcgctct ccggtggcag ggccgatatg cgcgatctcc
     2041 aggcggcggc aaataatggc gacgggcagg cccggcttgc catcgagact ttttgcagtg
     2101 ccatcagcaa ggtggtggcg ggttatgccg ccgcgctggg tggtttggac ttgcttgttt
     2161 tcacaggcgg cattggcgag cacagcgccc gcgtcagaaa ggatgtttgc caggatttga
     2221 aatttctcgg cgtctcgatc gacgattctg aaaaccagct tcaccgcggc acgatttcaa
     2281 ccacgcaaag cggcgttcgt gtgcgcgtcg tttcaagtca ggaaggccgc cagatcgccc
     2341 gtcattgccg cgccatgatg cgcagccccg tttcaggagt ttaaattatg acaatcgctc
     2401 aaaatgctga acggcaggtt gccggctccg cgcagccgct tgcaccggat gacctgcgca
     2461 agatcgatgc ctactggcgc gcctgcaact atctttgcgc gggcatgatc tatctgcgcg
     2521 acaacccgct tctgaaggag ccattgcggc cggcg
//
LOCUS       DATCXU010000431         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_823795,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000431 DATCXU010000000
VERSION     DATCXU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823795"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VIR04_09690"
     CDS             complement(<1..403)
                     /locus_tag="VIR04_09690"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY9461063.1"
                     /translation="MIVLIDAGNTRVKFGSVLPDGQRELVPLALRHTGLDRLSDWVRK
                     LPQAPTAALGVNVAGAAAAAAIEARLAAHGCPISWINSQNQAGGVRNAYDRASQLGAD
                     RWVSLIGLAWHARGRTGPDEHNNGTRRVAVRD"
     gene            complement(415..1257)
                     /locus_tag="VIR04_09695"
     CDS             complement(415..1257)
                     /locus_tag="VIR04_09695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY9461064.1"
                     /translation="MLSPDQMMVGLHEVLPHFKQIEWYERTASTNADLLTKARTSELR
                     LARPWLLGSHLQDLGRGRAGRRWQNRSGANLMFSCAFDVFLQPRQLPALSPLAGVAAC
                     EALRGLLHPQHRDQMVMKWPNDIQWKFSKLAGILVEVTRASTSRLSSDHHVVIIGIGL
                     NLNDARALSQSLNRQIADWAEITAEDDWAAAVSTPVMAGAIAQAWYKSLNDAIRQGFS
                     SFPERYANVDALAGQYINVLDNGRIANAGIACGVNSLGQLMVRTPDGQVPVSVGDVSV
                     RPQN"
     gene            1423..2067
                     /locus_tag="VIR04_09700"
     CDS             1423..2067
                     /locus_tag="VIR04_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9461065.1"
                     /translation="MPAFVKNKSFAGLAGVIDCTLDWPDATPEGWALVLHPHPLHGGA
                     RDNKVVTTLARACVDHGLVAVRPNFRGVGLSAGEFDGSIGETADMRALVEQFSQEYPK
                     LAAAKWVLAGFSFGTSVAAQLYSGLAERSEKTPGLLMLAGTAVQRFAPESVSVPGDTF
                     LIHGELDEVVPLSEVMDFARGRDLPVVVIPGASHFFHGKLIVLRQLAQQRLAAL"
BASE COUNT          413 a          646 c          697 g          527 t
ORIGIN      
        1 gatcccgaac tgccacgcgc cgcgtgccgt tgttgtgctc gtccggtccc gttcggccac
       61 gggcatgcca ggccagtcct atcagggaca cccagcgatc ggcgcccagt tggcttgccc
      121 ggtcgtacgc gttgcgcact cctcccgctt gattttgact gtttatccag cttattgggc
      181 acccatgggc agccagccgc gcctctatcg ccgcagcggc ggccgcgccg gcgacattga
      241 cgcccagcgc cgcggtgggg gcctgcggaa gctttcgcac ccaatcggac agccgatcca
      301 ggccggtgtg gcgtagcgcc agcggaacaa gttcacgctg cccgtcaggc aggactgaac
      361 caaatttgac ccgggtattg cccgcgtcga ttaaaacgat catgcttgag atattcagtt
      421 ttggggccgt accgagacat cgcccacaga aacggggact tggccgtcgg gagtgcgcac
      481 catcagttgg cccagcgaat tcacgccgca agcgatgccg gcattggcaa ttctaccgtt
      541 atcaagcaca ttgatgtact ggccggccaa cgcgtcgaca ttggcatagc gctcggggaa
      601 actgctaaag ccttgtctta tcgcgtcgtt caagcttttg taccatgcct gggcgatggc
      661 gcccgccata acgggcgttg acacggccgc cgcccaatcg tcttcggctg taatttcggc
      721 ccagtcggcg atctggcggt taagcgactg gcttagcgca cgcgcatcat tcagattcag
      781 gcctatgccg ataatgacca cgtggtggtc ggaagaaagc ctggacgtgc tggcccgtgt
      841 cacttcgacc agaattccgg ccagtttgga aaacttccac tggatgtcgt ttggccactt
      901 catgaccatt tggtctctgt gctggggatg cagcaggccg cgcagcgcct cgcacgcggc
      961 cacgccggcc aggggcgaca gcgccggcag ttgacgcggc tgcaggaaca cgtcgaaggc
     1021 gcaggaaaac atcaggttgg cgcctgatcg attttgccac cggcggcccg cccggccccg
     1081 cccaaggtct tgcaggtgcg accccaacag ccaaggtcgc gcaagccgca gttcgctggt
     1141 gcgcgccttg gttaacagat cggcattggt cgaggcggtt cgttcgtacc attcaatttg
     1201 cttgaagtgc ggcaaaacct cgtgcaagcc gaccatcatc tggtcgggag aaagcattgc
     1261 agcggtcgac aagctaagca ttatgtgtag aatgaagggt agggatgagg tgatttgggg
     1321 gcagtttgga ttgagcccag cgtacgcgtg acggcggtcc gtcgccatta gccatttgcc
     1381 gtctctcatt ttatcttatt ggccgcgtac tcgttaaaaa ctatgcctgc ttttgtaaaa
     1441 aacaagtcct ttgcgggcct ggctggcgtg atcgactgta cgctggattg gcccgacgca
     1501 acgccggaag gctgggcttt ggtgctgcat ccacaccctt tgcatggcgg cgcgcgcgac
     1561 aataaggtgg tgaccacgct tgcccgggcc tgtgttgacc atggcctggt ggcggtgcgc
     1621 cccaactttc ggggcgttgg tctgtcggcc ggcgaattcg atggctccat cggagaaacg
     1681 gccgatatgc gtgccctggt cgaacaattt tcccaggaat atcccaaact tgcggcggcc
     1741 aagtgggtat tggccgggtt ttcatttggc acatcggtgg cggcgcaact gtattcgggc
     1801 ttggccgagc ggtcggaaaa gactccgggg ctgttgatgc tggcgggaac ggctgtgcag
     1861 cgttttgcgc ccgaatcagt ttcggtgccg ggcgacacat ttttgattca tggcgaactc
     1921 gacgaagtcg tgccgctgtc tgaagtcatg gatttcgccc gtggccgcga tttgccggtg
     1981 gtggttattc ccggcgcgag tcattttttt cacggcaaat tgattgtatt gcggcaactg
     2041 gcgcaacagc gcctggcggc attgtaggtt tttgtattaa ttattttcag ttttaaaggt
     2101 taaattaaaa cgcgccgcct ttgcgtccgg caggtgctgc ctataataag gcattctttt
     2161 tttcatcatc tactggcgag cccgttttca caatatggca atgacatcga ccgttccgtg
     2221 tttggcccgg ctgcgttccg gctttgtatt ggcggttgcc gcgctggctt ttgggccatt
     2281 gat
//
LOCUS       DATCXU010000432         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_597523,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000432 DATCXU010000000
VERSION     DATCXU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597523"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VIR04_09705"
     CDS             complement(<1..318)
                     /locus_tag="VIR04_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY9461066.1"
                     /translation="MGFLNGKRVLITGVISNRSIAYGIAHACRQQGAELAFTYVGERF
                     ESRVRELAAELDSKIVLACDVADDAQIQSCIGELQTHWDGLDGLVHSIGFAPREAIAG
                     AFLD"
     gene            complement(349..1728)
                     /locus_tag="VIR04_09710"
     CDS             complement(349..1728)
                     /locus_tag="VIR04_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY9461067.1"
                     /translation="MIYLRLFILLITAVLAGCATNNTNQPNASAKRTYTAAETSRTID
                     LTHPPRDMWDRIRRGFSIPDLHNDLTQQWTDYYASHPASIVLMSQRASKYLYYIVNEL
                     DRRGLPTELALLPFVESAYDPTAYSRSNASGLWQFIPSTGQHYNLQQDRWRDQRRDPI
                     ASTNAALDYLSYLYDFQGDWYLALASYNWGEGAVRRAMDRNTGNETLPDYSSINMPAE
                     TRNYVPKLQAIKNIIANPKKYGITLPEISNKPYFAKVHKSRDMDIAVAARLAEMSVED
                     FKSLNASFNRPIMLGEHNPTLLLPTNRVDIFNANLEAYKGRLSSWDTYKAKIGESYAS
                     IAKQHGISLKKLRQINGLTGKTNRAEAQTLLIPASDTQGGVQLASMKGSDEEESAVIP
                     DRRQRDIIVIRRKANVRTHTVKPGDTLYSLAARYHTSVDALRKLNNLKNTNLAKGARL
                     RVPGTDIRG"
     gene            complement(1736..2548)
                     /gene="gloB"
                     /locus_tag="VIR04_09715"
     CDS             complement(1736..2548)
                     /gene="gloB"
                     /locus_tag="VIR04_09715"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY9461068.1"
                     /translation="MKPIFNQDKNQTQLIPVPALSDNYIWMVRKNGHALVVDPGESAP
                     IEKILEQHSFKLDAILLTHHHGDHVGGARALQQLTGAAIYGPATETLPACDHPLKQSD
                     TVKLKNLDVTFSVLDIPGHTSGHIAYFGYLEPDRPLVFCGDTLFAAGCGRLFEGTPTQ
                     MADSLGKLAALPLETLVCCGHEYTLANLRWALTVEPGNRALHERNEAALRLRSNGLPT
                     LPSTIGAELQTNPFFRTTQESVTAAAQAYAGVPLNSAVAVFTHLREWKNNFK"
     gene            2570..3331
                     /locus_tag="VIR04_09720"
     CDS             2570..3331
                     /locus_tag="VIR04_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY9461069.1"
                     /translation="MSLDRPLIVELAEWLISPSGQYVLEWEQKQFDAIVANVFGYHAI
                     QIGLPQWDVLRTNRIPYKGRTHAIHDAGLDSKVVSLVADPENLPFDTQSIDLLVLPHT
                     LECSDHPHQILREAERVLVPEGRVVISGFNPWSLWGGRERIPGLDPLMPVPAHLQVSL
                     ARLKDWFKLLSFEVDRGRFGCYAPHCLSKVWLDRWAFMDAAGDRWWPVCGAVYVVSAV
                     KRVVGLRMIGPEWKTKKKRVHHAAVVTGCRSELEL"
BASE COUNT          677 a          830 c         1017 g          814 t
ORIGIN      
        1 gtccaggaag gcgccggcga tggcttcccg aggtgcgaat ccgatggaat gcaccagccc
       61 gtcaaggccg tcccaatggg tttggagttc acctatgcag ctttgaatct gtgcgtcgtc
      121 ggcgacgtcg caggcaagca caattttgct gtccagttcc gccgccagtt cgcgcacccg
      181 gctttcaaac cgctcgccca cataagtgaa ggccagctcg gcgccttgct gccggcaggc
      241 atgtgcgatg ccgtatgcaa tcgaccggtt ggatataacg cccgttatca agacccgctt
      301 gccgttcaga aaacccatgt tgtcaatcct ttgttgccac tacgaaaatt agccgcgaat
      361 atcggtgccg gggacgcgca gccgtgcgcc cttggccaag ttggtatttt ttaaattgtt
      421 cagcttgcgc aaggcgtcga cggacgtatg gtagcgcgca gccagcgaat acagggtgtc
      481 gcccggcttg acagtgtggg tgcgcacgtt ggctttgcgt cttatcacaa tgatgtcacg
      541 ctggcggcga tcgggtatga cggccgactc ttcctcgtcg gagcctttca tcgaagccag
      601 ttgaactccc ccttgcgtgt cgctggcggg gatgagcagc gtttgcgcct cggcgcgatt
      661 ggttttcccg gtcaggccgt tgatctggcg caattttttc aagctgatgc cgtgttgctt
      721 ggcgatactt gcgtacgatt cgccaatttt ggctttgtag gtatcccaag agctcagtct
      781 gcccttgtag gcttcaaggt ttgcgttgaa aatatcgacg cggttggtgg gcagcaacag
      841 ggtgggattg tgttcgccca acataatggg gcggttgaac gacgcattaa gcgatttaaa
      901 gtcttcgacc gacatttccg ccaggcgcgc cgcaacggca atatccatgt cgcgcgattt
      961 gtgcactttg gcgaagtaag gtttgttgct tatctctggt agcgtaatgc cgtatttctt
     1021 ggggttggca ataatattct tgatggcctg caatttgggt acgtagttgc gggtttcggc
     1081 aggcatattg atggacgaat aatcgggcaa agtctcgttg ccagtgtttc gatccatggc
     1141 gcgccgcact gcgccctcgc cccaattgta cgaagccagg gccagatacc agtcgccctg
     1201 gaaatcataa aggtaactta aataatcaag cgccgcattg gtagaggcga tggggtcgcg
     1261 acgctggtcg cgccaccggt cctgttgcag gttgtagtgc tgaccggtgc ttggaatgaa
     1321 ctgccacagc cccgacgcgt tggagcgtga ataggcagtc gggtcgtagg cgctttccac
     1381 aaaaggcagc agtgcaagtt cggtgggcag tccgcgccga tccagctcgt tgacgatgta
     1441 gtatagatat ttgctggctc gctggctcat gagcacgatg gaagcggggt gcgaagcgta
     1501 gtagtcggtc cattgctgcg tcagatcgtt atgcagatcg ggaatggaaa agccgcggcg
     1561 aatgcggtcc cacatgtcgc gcggggggtg ggtcagatcg atggttcgcg atgtttcggc
     1621 tgcggtgtag gttcttttgg ccgacgcgtt cggctgattt gtattattgg tcgcgcaacc
     1681 ggccaaaact gctgttatca gcaggatgaa tagtcgcagg tatatcatcg gcaattcatt
     1741 taaagttgtt cttccattca cgcaagtggg taaagacagc tacggccgaa tttagcggga
     1801 cacccgcata agcttgagcg gcggctgtta cactttcttg tgtcgtgcga aaaaaagggt
     1861 tggtctgaag ctcggcgccg atggttgacg gcaaagtcgg taaaccattg gaacgaagcc
     1921 gcaaagcagc ctcgttgcgc tcatgcagag cccggttgcc gggctctacc gtcagagccc
     1981 atcgtaaatt tgccagagtg tactcgtgac cacaacaaac aagtgtttcc agtggtaaag
     2041 cggcaagttt acccaaagaa tcggccattt gggtaggggt gccttcaaat agtcgcccgc
     2101 aacctgcggc aaacagtgta tcaccacaga aaacaagggg ccggtccggc tccagatagc
     2161 caaagtaggc gatatgcccc gaagtgtgcc ccggaatgtc caggacagag aaagtcacgt
     2221 ccaggttttt taatttcacg gtgtcgcttt gtttaagcgg gtggtcgcat gcgggcagtg
     2281 tttctgtagc gggtccgtaa atggccgcgc cggtcagttg ttgcaatgcg cgcgcgccgc
     2341 cgacatggtc accgtgatgg tgagtgagta gaatggcatc cagtttgaac gaatgttgtt
     2401 caaggatttt ttctatgggc gccgattcgc ctggatcaac gaccagcgca tgaccattct
     2461 tgcggaccat ccatatgtag ttatcggata gcgcgggaac gggaatcaac tgcgtctggt
     2521 ttttgtcttg attaaaaatg ggtttcatcc taaaacaaca ggaggcgatg tgtcgctgga
     2581 ccgacccctt attgtagagc ttgccgagtg gttaattagc ccttccggac agtacgtgct
     2641 ggagtgggag cagaagcagt tcgacgctat cgtggcgaat gtatttggct atcatgccat
     2701 ccagattggt ttgccgcaat gggacgtact tcggaccaac cgtattcctt ataagggccg
     2761 gacgcatgca attcatgacg ccgggctgga ttccaaagtc gtgtcgctgg tggccgaccc
     2821 tgaaaacctt ccgtttgata cgcaaagtat cgacctgctg gtattgcctc acacgctcga
     2881 gtgttccgat cacccgcatc agattttacg cgaagccgaa cgggtgttgg tgcccgaggg
     2941 gcgcgtggtg atttcggggt tcaatccctg gagtttatgg ggagggcgcg agcgcattcc
     3001 cggcctcgat ccgctcatgc cggtcccagc tcatttgcag gtgtcgctgg cgcggctcaa
     3061 ggattggttc aagcttttgt ctttcgaggt cgatcgcggt cgttttgggt gttacgcgcc
     3121 gcattgtctg agcaaggtat ggcttgaccg atgggctttt atggacgcgg ctggtgatcg
     3181 ctggtggccg gtgtgcggag ccgtttacgt cgtttccgcg gtcaagcgtg tcgtcggatt
     3241 gcgaatgatc ggtcccgagt ggaaaaccaa aaaaaagcgg gtgcatcacg cggcagttgt
     3301 aacgggctgc cgctcggaac tggaactata ggatgtgt
//
LOCUS       DATCXU010000433         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_64624,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000433 DATCXU010000000
VERSION     DATCXU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64624"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..356
                     /locus_tag="VIR04_09725"
     CDS             84..356
                     /locus_tag="VIR04_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY9461070.1"
                     /translation="MRYSNNIRPISYLKEKTTAIIREITETREPMVITQNGNATFIVQ
                     DIKSFEQTQETMALLKILALGQNEITTGKFSSSDDFFRQMDKEIME"
     gene            353..694
                     /locus_tag="VIR04_09730"
     CDS             353..694
                     /locus_tag="VIR04_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY9461071.1"
                     /translation="MTSYKVVVLEGAQKELRRLYSGYLLAEFGKAVAQKEYTAIKKSV
                     NLLKTNPEMGTQIPGLSDLGFNHYRQMTVQRLNKIVYQIDRSHKTVYVHIFCSARQEF
                     QTILNQRLLMD"
     gene            complement(740..1930)
                     /locus_tag="VIR04_09735"
     CDS             complement(740..1930)
                     /locus_tag="VIR04_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY9461072.1"
                     /translation="MKIKNIETLACDAGWRNYNFLKVITDDGIVGWSEYDETYGPPGL
                     TAVIERYAARLIGKPVYDHEQIYETLAATARPAPYGLSAEAFGAIENALLDAKAKSLG
                     VPCYELLGGKHRDAIPIYWSHCTTWRVNFPELYPPAITDLDGVRKAGEGARKLGYSAV
                     KTNMFTHNDGKIQQWMGGFGAPHSPSLNIDKPLIRSVRAHLEALRDGAGPDMEILIDL
                     NFNARTEGFVRMIRALEDFELFWVELDIYNPAALAYIRQQSRHPISSCETLFGIRQFL
                     PYFQQQSMDVAIIDAVWNGVWQAMKIAHTANAFDVNIAPHNFYSHMATMMNVHFAAAV
                     PNLRIMEQDGDRLPWDDELVTCPPKIENGAIIVPQTPGWGCEPNEEALRAHPAKIMRG
                     YLGL"
     gene            complement(1960..2889)
                     /locus_tag="VIR04_09740"
     CDS             complement(1960..2889)
                     /locus_tag="VIR04_09740"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY9461073.1"
                     /translation="MKIVMMGSGGVGGFFGGRLAQVGYDVTFIARGAHLAAMQKQGLL
                     IESEAHGDIHIPKVRATDDPATLGQADLVIVSVKLWDSDATAKLIKPLVGPKTGVLSL
                     QNGVIKDEIWRGAFGEKAVMGGVAYVGSHIARPGVIHQVGTMQRVVLGEYDGRKSERA
                     EFLYEALKHSGVTAELSTDVRRAIWEKYVFLVGLSATTASMRTSLGPIRSNPKSRAFL
                     LQLMQEVVAVGRACGVAIPEDFAEGRLAFADSLPADMNSSMNHDLQTNRPLEVNWLSG
                     GVVKLGKEKGVPTPANSAVCDILALYADGPVVA"
     gene            complement(2954..>3638)
                     /locus_tag="VIR04_09745"
     CDS             complement(2954..>3638)
                     /locus_tag="VIR04_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY9461074.1"
                     /translation="PAAMSESVPATQADSTLSGIKKAFEARFPGVEVSAVTPTPYAGL
                     YEIQLGTDVLYTNAQVDYVLQGSLVDSKTRVDLTAERLDKLSRIAFDSLPLKLAVKEV
                     KGNGSRKLAAFEDPNCPYCKQLHKTLEQLDNYTFYTFLFPILTPDSEVKARDIWCAKD
                     QGATWRDWMGNAKTPATAKCDTPVADVLALGKKLAIQGTPTLIFADGSRVNGTLPLDQ
                     LKEKLDSLK"
BASE COUNT          829 a         1010 c          955 g          844 t
ORIGIN      
        1 cttttccgtt aaggcaatga attgccgcca aactaaattt agactatatt cagttgtatc
       61 tttacctgtt caggggtact cgcatgcgtt actcaaacaa tatccggcca atcagttacc
      121 ttaaagaaaa aaccacggcc atcatccgcg agatcaccga aactcgtgaa ccaatggtca
      181 ttacccaaaa cggcaatgcc acgtttatcg tgcaagatat caaaagtttc gaacaaacac
      241 aggaaacaat ggcgcttttg aagattcttg cactcggaca aaacgaaatc acaaccggaa
      301 agttttccag ctcggatgac tttttcagac aaatggataa ggaaatcatg gaatgacttc
      361 ctataaggta gtcgttctcg aaggcgctca aaaggagtta cgacggttat acagtgggta
      421 tttgttagcc gaatttggca aggctgttgc acaaaaggaa tacacggcca ttaagaaatc
      481 cgtaaatttg cttaaaacaa acccggaaat gggaactcaa atacccgggc tatcggatct
      541 tggatttaat cactatcgac agatgaccgt acagcgcctg aacaaaatcg tctatcaaat
      601 agaccgctca cacaagactg tgtacgtgca tattttttgt tcggcgcgcc aagaatttca
      661 aacaatatta aatcaacgac ttttaatgga ctgaaaggtg ttttaaaacg gcccttgatc
      721 aagagccgtc cgcgccgcct tacaacccca gataccctct catgatcttt gccggatggg
      781 cgcgtaacgc ctcttcgttt ggctcacatc cccaccccgg tgtttgcgga actatgatgg
      841 cgccattttc gatttttggc gggcaagtga ccagttcgtc atcccagggc aggcgatcgc
      901 catcctgttc catgatgcgc aagttcggca ctgccgccgc aaaatgcaca ttcatcatgg
      961 ttgccatgtg gctgtaaaag ttgtgcggag caatattgac gtcgaacgcg ttggcggtgt
     1021 gggcgatttt catggcctgc catacgccat tccatacggc gtcgatgatg gcgacgtcca
     1081 ttgactgctg ctgaaaataa ggcaggaact ggcgtatgcc aaacaacgtt tcgcaggaac
     1141 tgatcgggtg acggctttgc tgacgaatgt aggcaagcgc tgcggggttg taaatgtcga
     1201 gttcgaccca gaacagttca aaatcctcca gcgcacggat catgcgcacg aacccttcgg
     1261 tgcgcgcatt gaagttcaag tcgatcagaa tttccatgtc gggaccggcg ccgtcgcgca
     1321 gcgcttcaag atgcgcgcga acgcttcgga tcagcggctt gtcgatattc agcgacggcg
     1381 aatggggcgc gccaaacccg cccatccact gctgaatttt cccgtcatta tgcgtaaaca
     1441 tattcgtttt cacggccgaa tagcccagtt tgcgggcgcc ttcgcccgct ttcctgacgc
     1501 catccagatc ggtgatggcc ggcggataca gttcgggaaa attaacgcgc caggtcgtgc
     1561 agtgcgacca atagatggga atggcgtcgc gatgctttcc acccagcaat tcgtagcagg
     1621 gcacgcccag cgactttgcc ttcgcgtcga gcaacgcatt ttcgatggcg ccgaacgcct
     1681 cggcgcttaa accatatggc gcgggccgcg ccgtcgcggc cagggtttcg tagatctgtt
     1741 catgatcgta gaccggcttg cctatcaggc gagcggcata acgctcgatg acggccgtca
     1801 aacccggagg gccgtatgtt tcgtcgtatt cgctccaacc gacgattcca tcgtcggtaa
     1861 tgactttaag aaaattgtaa ttgcgccagc cggcgtcgca ggccagagtc tcgatatttt
     1921 tgattttcat gccttgtgtc ccctccttga agatcgttgt caggccacta ccggaccatc
     1981 cgcgtacaag gcaagaatgt cgcaaactgc gctattggcg ggcgttggca cgcctttttc
     2041 tttgcccaat ttaaccaccc cgccgcttag ccaattcacc tcaagcggcc gattggtttg
     2101 caagtcatgg ttcatggagg agttcatgtc cgccggaaga ctgtcggcaa acgcaagacg
     2161 tccttcggcg aagtcttcgg gtattgcgac gccgcacgcg cggccgacgg caacgacttc
     2221 ttgcatgagc tgcaataaga atgcacggct tttggggttg ctgcgaatcg ggcccagcga
     2281 agtgcgcatg gaagcggtag tcgccgacag cccaaccaga aacacgtatt tttcccagat
     2341 ggcgcggcgc acgtccgtgc taagttcggc tgtaaccccc gaatgcttca gggcttcgta
     2401 aaggaactcg gcccgttcgg acttgcggcc atcgtattcg ccaagcacca cgcgttgcat
     2461 cgtgccgacc tgatgaataa cgccgggccg cgcgatatgg ctgcccacgt aggcaacgcc
     2521 gcccatgacg gctttctcac cgaacgcgcc acgccagatt tcgtctttga tgacgccatt
     2581 ttgcagcgaa agcacgccgg tcttggggcc gaccagcggt ttgatcaact tggccgtcgc
     2641 gtcgctgtcc cagagcttga ccgagacaat gaccaggtcg gcctgcccga gcgtcgccgg
     2701 gtcgtcggtg gcgcgaactt tcggtatatg aatatcgcca tgcgcctcgc tttcgataag
     2761 caagccctgc ttttgcatgg ccgccagatg cgcgccacgc gcgataaaag taacgtcgta
     2821 acccacttgc gcaagccggc cgccgaagaa gcctcccact ccacccgatc ccatcatgac
     2881 tattttcatt agaactccag aagtcttgtg tgcataaatg aaaaaccgat ttgattttaa
     2941 tgttacttcg gtgttacttg agggaatcca gcttttcctt taactggtcc agcggcaagg
     3001 tgccattcac gcggctgcca tcggcaaaaa tgagtgttgg ggtgccttgt atcgccaact
     3061 ttttgcccag agccaggaca tcggcgactg gtgtatcgca cttggccgtc gccggcgttt
     3121 tggcgttacc catccagtcg cgccacgttg cgccctggtc tttggcgcac cagatatcgc
     3181 gcgccttcac ttctgaatcg ggcgtcagga tagggaagag aaatgtataa aaggtatagt
     3241 tatccagttg ctcaagggtt ttatgcagtt gtttgcagta ggggcaattt gggtcttcga
     3301 aagcggccag cttgcgcgag ccattgccct tcacttcttt aaccgcaagt ttcaacggca
     3361 gcgagtcgaa tgcgatgcgg gaaagcttgt ccaatcgctc cgccgtcaaa tcgacccggg
     3421 tcttgctgtc caccagcgaa ccctgcaaaa catagtcgac ctgcgcgttg gtgtacaaca
     3481 cgtccgtgcc cagttgtatt tcgtacagac ccgcgtacgg cgtgggggtc accgcgctga
     3541 cttcaacccc cgggaatcgc gcttcgaacg cctttttaat cccgctcagc gtgctgtcgg
     3601 cctgcgtggc gggaaccgac tcggacatgg ccgcgggg
//
LOCUS       DATCXU010000434         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_912390,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000434 DATCXU010000000
VERSION     DATCXU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912390"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VIR04_09750"
     CDS             complement(<1..487)
                     /locus_tag="VIR04_09750"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY9461075.1"
                     /translation="MTDLAKKLPVAEAGIKSIEVGCRLLDALANIRHPINLGELALLS
                     KMSPSKAHRYLVSFQRTKLVQHDAQTHRYALGELALKLGYSAAAQANTFRQLQRVQVA
                     LRDKTTHTVLLSVWTDGGPVVMGVEDGLSPVVATMKPGATLPLMSSAAGRVFAASMPW
                     RI"
     gene            complement(618..1649)
                     /locus_tag="VIR04_09755"
     CDS             complement(618..1649)
                     /locus_tag="VIR04_09755"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEY9461076.1"
                     /translation="MAKPSKFAELIQTVTKDVIGHPRDLTRHYANRFGISRVSANKYI
                     QRLETEGWIARSGPSTHPVFSPGYKRRVSKLYSLNGLEEHVVWEQDFRPYFSLTPNVQ
                     NIANHGFTEMVNNAIDHSGGESVFAWMSQDESAIYISVSDNGIGIFAKITNALNLPDM
                     RQALFELSKGKLTTDPSKHTGEGIFFTSRMFDSFDITANDLRFNHSDISPTDWLGEDK
                     AFFREGTAVFMRIALDSQRTAADVYAQFTDVPEDYAFSKTEVPMKLARSGDEQLVSRS
                     QAKRLIARFDRFKIVTLDFDGIQEIGQAFADELFRVYGAAHPEVELLPKNMSEQVERM
                     WLRTVSPNI"
     gene            complement(1756..>2791)
                     /locus_tag="VIR04_09760"
     CDS             complement(1756..>2791)
                     /locus_tag="VIR04_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY9461077.1"
                     /translation="TEEDEMRAIQIEEYGGTNVLKQRDVAVPVPGKDEVLVKLAYAGI
                     NFMDVYTRLGNYARSGHYQATLPLTLGMEGAGRIKACGPGVIGFKPGDRVAYCLANGS
                     YADYAIVPAWKIVKVPDGLSLKLAAASLFQGLTAHYLVHDVANLKSGDSCLVHAGAGG
                     IGQIVIQLVSRQGVHVIATASSPEKADIARQCGAERVCAYDPESVTSAVKAMTAGRGV
                     DVVFDSVGAATFQISMHALKKAGLLIAYGANSGPFSSIDPMELANNGSLFFTRPRLAD
                     YVPDAASIRRRADNIFAGLMDGRLNVHIDKVFDFEAVAAAHGELEERRSIGKSVLCIN
                     AGEDCGFGDD"
BASE COUNT          633 a          762 c          728 g          668 t
ORIGIN      
        1 tgatgcgcca aggcatcgaa gctgcgaaaa cccgtccagc ggcgcttgac atcaaaggta
       61 gcgtcgcacc gggcttcatg gtcgcgacaa cgggcgataa tccatcttcg acgcccatca
      121 cgacaggtcc cccgtccgtc cagacggaga gcaatacggt gtgtgtggtc ttgtcccgaa
      181 gcgccacctg aacgcgctga agttgccgga aggtattggc ctgggccgcc gccgagtacc
      241 ccagtttcaa agccaactcg cccagtgcgt agcggtgagt ctgagcgtca tgctgcacca
      301 atttcgttcg ctgaaaactg accagatagc ggtgcgcctt gctgggcgac attttgctga
      361 gcagcgcaag ctcgcccaag ttgatcggat gccgaatgtt tgccagtgca tccagcaatc
      421 tgcagccgac ttctatcgac ttgatgccgg cttccgccac aggtaatttt ttagcgaggt
      481 cagtcatgtg tcgcgagcag gccatcatcc aattggttga tacaggtggc atattgtcac
      541 ataaattttt ccgcagatca ggggacttgg ctgcagcgac atagcgcaac gattcgccat
      601 ttcttcactg gaacccgcta aatatttggc gagacagtgc gtaaccacat gcgttcaacc
      661 tgttcgctca tattcttggg cagtagctcg acttcaggat gcgcggcgcc atagacgcgg
      721 aagagttcgt ccgcaaaggc ctggccaatt tcctgaattc cgtcgaagtc gagggtgaca
      781 atcttgaagc gatcaaacct ggcgatgagt cgtttggcct gggaccggga gaccaactgc
      841 tcgtccccgg acctggcaag tttcattgga acttcggtct tcgagaaggc atagtcctcc
      901 ggcacgtccg taaactgggc gtaaacatcg gccgcagtgc gctgactgtc tagtgcaatt
      961 cgcataaaga cggcggtgcc ttctcgaaaa aaagccttgt cttcccctag ccaatcggtt
     1021 ggcgaaatgt cactgtgatt gaaacgcagg tcgtttgctg tgatatcgaa cgaatcaaac
     1081 atgcgggaag tgaaaaatat gccctctccc gtgtgttttg aggggtctgt tgtcagtttt
     1141 cccttcgata actcgaagag cgcttgacgc atgtctggca gattcaaggc gttggtgatc
     1201 ttcgcaaaaa taccgatacc attgtccgag acgctaatat aaatggcaga ttcgtcttgg
     1261 ctcatccacg cgaatacaga ttctcctccc gaatgatcaa tagcgttgtt aaccatttcc
     1321 gtgaatccgt ggttcgctat attttggacg ttgggcgtga ggctgaagta gggtctaaag
     1381 tcctgttccc atacaacgtg ttcttcaagc ccgttcaacg agtaaagttt tgagacgcgt
     1441 cttttgtaac ctgggctgaa aacgggatga gttgatgggc cactgcgagc gatccacccc
     1501 tctgtttcaa gtcgctggat atacttgttg gccgaaactc gtgatatgcc gaaacgatta
     1561 gcgtaatggc gagtgaggtc gcggggatgc ccaatcacgt cttttgttac ggtctggatc
     1621 agttccgcaa acttggaggg cttggccatc aagtctcaat tgttaactta aataatgatc
     1681 attgtaaact tataaatgga atgttgtaaa ccattcgtga acacgattct gctggtaatg
     1741 agctacttcc ttaagtcagt catcaccaaa gccgcaatct tcaccagcgt taatacacag
     1801 cacggattta ccgatggaac ggcgttcttc cagctcgcca tgcgccgccg ccacagcttc
     1861 aaaatcgaag accttatcaa tgtgcacgtt taaccggcca tccatcaatc ccgcaaaaat
     1921 gttgtcggcg cgccgccgta tcgatgcggc gtctggcaca tagtcggcca atcgcggccg
     1981 ggtgaaaaat aatgatccat tgttggccag ctccatggga tcaatggagg agaacggccc
     2041 cgagtttgcg ccgtacgcaa tcaataagcc ggcttttttc aacgcgtgca tgctgatttg
     2101 aaatgtggcc gcgcccacgg aatcaaacac cacgtccact cctcgtcccg ctgtcattgc
     2161 tttcacggca gatgtaaccg attccggatc atacgcgcat acccgttccg cgccgcactg
     2221 ccgcgcgatg tcggcttttt cgggagagct ggccgtagcg attacgtgca caccttgccg
     2281 cgagacaagt tgaatcacta tttgccctat gcctcccgcg ccggcgtgca ccagacagct
     2341 atcgccggat ttcagattgg cgacgtcgtg caccagataa tgcgcagtca gcccctgaaa
     2401 tagcgatgcg gccgccagtt tcagacttaa tccgtcgggg accttaacga ttttccaagc
     2461 gggcacgatg gcatagtcgg cgtagctgcc attggccaga cagtaggcca cgcgatcccc
     2521 aggcttgaat ccgatgacgc ccggaccgca ggctttgata cgccctgcgc cttccatgcc
     2581 aagggtcaat ggcaaagtgg cctggtaatg tcccgaacgg gcatagttac ccaagcgggt
     2641 ataaacatcc atgaagttga tgccggcata ggccagtttt accaatactt cgtctttgcc
     2701 tggaaccggc actgcaacgt cgcgctgctt aagcacattg gtgccgccgt actcttcgat
     2761 ctggattgct cgcatttcat cctcttccgt t
//
LOCUS       DATCXU010000435         5148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_283030,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000435 DATCXU010000000
VERSION     DATCXU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283030"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VIR04_09765"
     CDS             complement(<1..247)
                     /locus_tag="VIR04_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461078.1"
                     /translation="MPTPALPPLYKAELSPISSPAQVRTALWRRIWRNKALTAGGAVL
                     LIILLLAIFAPWISPHDPYAQDLAHRVVPPVWYDKGSW"
     gene            complement(251..1168)
                     /locus_tag="VIR04_09770"
     CDS             complement(251..1168)
                     /locus_tag="VIR04_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461079.1"
                     /translation="MLAYVIKRLGLAILVALTVSIIAFLLLNLAGDPAAAMAGEGARQ
                     ADIEMIRHAYGLDRPLTVQYGDWLWKMLHGDFGMSIYFKTAVGPLIFDKLRTTVLLGL
                     LALCFSLVISIPLGVAAALYKNSLIDRVCLAIAVFGQALPSFFFALLLIMVFAVSWRI
                     LPVSGSGTWQNFILPAVALGYYVAPAFMRLIRAGMIEVLGADYIRTARAKGLPARSII
                     FKHALRNAIIPVVALAAVQLGYLLGGSVVIETIFALDGLGYLAFQSITYKDYPVMQVI
                     VLILSIIYVLLTLASDIANAWLDPRIRAS"
     gene            complement(1193..2758)
                     /locus_tag="VIR04_09775"
     CDS             complement(1193..2758)
                     /locus_tag="VIR04_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461080.1"
                     /translation="MTEKPSSLIAALPHKFSFKTLAGSLLMASFLCVSTSALATKKDD
                     TLRMAYDQAPESVDPYFNNVRIGVIIAANVWDTLLYRDPLTNEYKGQLAKSWKQVDDK
                     TIDFDLREGIKFHNGEEFDADSVVYTLNFVADPKNKATTQANVSWIDKAEKLGKYKVR
                     LITKKPFPAAKEYLSTTVAIMPAKYYKEVGPAGMSAKPVGTGPYKVTAYQPGKSITLE
                     RNKDYFKDSPKAQPKIGKVVIRFIPDKQTQVAEVISGGEDFIMNVAKDQAEQLKSMPN
                     LHVSSGNTMRIVFLQLNSRDGGPAPELKDERVRKAIAHAIDRPSIIKNIVGGGAQLLN
                     TICSPSQVGCTSDGATVYKYDPELSKKLLAEAGYPNGFNVDILAYRERNQTEAIINYL
                     RAVGIKAKLNFLQYAAMRDVIYANKASITHQTWGSNLVNDVSASTPVFFGGGGYDVTR
                     DPAVVSLLNKGDNTIEPAARNEAYKKALDLISEHAYAVPLWTLPAYYVSNKDVNFTPL
                     PDELIRFWDMSWK"
     gene            complement(2841..>5148)
                     /locus_tag="VIR04_09780"
     CDS             complement(2841..>5148)
                     /locus_tag="VIR04_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY9461081.1"
                     /translation="FNPQSERPYTSDGSVNLAHLLVGSEGTLAYTQQITLKLSALPAH
                     KALGVVNFPTFYQAMDMAQHIVKLGPCAVELVDRTMIDLARENPAFKPVIDKAVIGEP
                     EAILLVEFAGEDNSEQLRKLAKLVELMGELGLPDSVVEMPNPAAQKALWAVRKAGLNI
                     MMSMHGDGKPVSFIEDCAVPLEHLAEYTNRLTEIFRKHGTRGTWYAHASVGTLHVRPI
                     LDMRTSGAEKMRAIAEEASDMVRKYKGAFSGEHGDGLARSEWISWQFGPRLTHAFEEI
                     KDLFDPEGMMNPGKIVRCPKMDNTSLFRFKPTYQVLPMRPAFDWSAWNVQNDPDKQTT
                     SAPGTGGDAAGGFGKAIEMCNNNGHCRKFDTGTMCPSYRVTRDEKHLTRGRANTLRLA
                     LSGQLGPDALTSGEMHETMDLCVGCKGCRRECPTGVDMAKMKIEFLYQWQKKYGLKLK
                     DRLIATLPHWAPWAARFPFLFNLRDTLPGAKMISEKWLGFSAKRSLPKWRRDTFLRDA
                     HSADAASANVVLFADTFNNYLESENSRAALNVLQAAGYRVHIARPLKNDAHANRPLCC
                     GRTYLSAGLVDEAKLEAKRVVAALRPFVTRGIPVVGLEPSCLLSLRDEFLVMGMGADA
                     TTLAKNSFLFEEFLAREQKAGTLHLKLKALPEKRALLHGHCHQKAFDAVKPVQVVLGL
                     IPELDVSLIESSCCGMAGSFGYEAKHYDISMKMAELSLLPAIRKADANTLLVADGTSC
                     RHQIADGTRDGVARKAEHVARVLERALA"
BASE COUNT         1027 a         1393 c         1527 g         1201 t
ORIGIN      
        1 gccacgagcc tttgtcgtac cagacgggcg gcacgacccg gtgcgccaaa tcctgggcgt
       61 agggatcgtg aggagaaatc catggggcga agatggccag cagcagaatg atcagcaata
      121 ccgctccgcc cgctgttaaa gccttgttgc gccagattcg ccgccataag gcggttcgaa
      181 cctgcgcagg acttgatatg ggcgataatt cggctttata cagtggcggc aaggcgggcg
      241 ttggcattat tcaggaagcc cgtatgcgtg ggtcgagcca ggcgttggca atatcggaag
      301 ccagcgttag caggacgtag atgatggata gaatcagcac aatgacttgc atgaccggat
      361 agtctttgta ggtgatgctt tggaaggcaa ggtatcccag cccgtccagc gcgaaaatgg
      421 tttcgatcac caccgagccg cctagtaaat agcccagttg caccgcggcc agcgccacaa
      481 ccggaatgat ggcgttgcgc agtgcgtgtt tgaagatgat gctgcgtgcc ggcagcccct
      541 tggcacgggc tgtgcggata tagtccgcgc cgagcacttc gatcatgcca gcccgaataa
      601 gccgcatgaa ggcgggcgcg acgtaatagc ccagagctac agcgggcaaa ataaaatttt
      661 gccatgtgcc gctgcccgac acgggaagaa tgcgccatga gacggcgaac accataataa
      721 gaagcagcgc gaagaaaaag ctgggcagag cctggccgaa aacagcaatc gcgaggcaca
      781 cgcggtcgat caggctgttt ttgtagagcg cggctgcaac acccagcggg atggatatca
      841 ctaaggaaaa acatagcgcc aacaacccaa gcaatacagt cgtgcgcagt ttgtcgaaaa
      901 taagggggcc gacggcggtt ttgaaataaa tagacatccc gaagtcgcca tgcagcattt
      961 tccagagcca gtcgccatac tgcacagtca aagggcgatc cagaccataa gcgtggcgga
     1021 tcatttcaat atccgcctgg cgcgcgccct cgccggccat cgcggccgcc gggtcgcctg
     1081 ccaggttcag caacaaaaag gcaataatgg agacggtgag cgccaccaat atggcgaggc
     1141 ccaaccgttt gatcacatag gccaacatca ggtttgtctt cgttggtttc gcttatttcc
     1201 agctcatgtc ccaaaagcga atcaactcat cgggaagggg cgtgaaattc acgtctttgt
     1261 tcgacacata gtaggccggc aaggtccaca gaggcacggc ataagcatgt tcggaaatga
     1321 gatcgagcgc tttcttgtat gcctcgttgc gtgcggccgg ctctatggtg ttgtcgccct
     1381 tgttgagcag tgagacgacc gccggatcgc gagtgacgtc atagcctccg cctccgaaaa
     1441 acacaggagt cgacgccgat acgtcgttga ccagattgga gccccaggtt tggtgcgtga
     1501 tcgacgcttt gttggcgtag attacgtcgc gcatggctgc gtattgcagg aaattcagtt
     1561 tggccttgat gccgaccgcg cggaggtagt tgatgatggc ttcggtctga ttgcgttccc
     1621 gataagccag gatgtcgaca ttgaagccat tggggtagcc cgcttcagcc aacagttttt
     1681 tggacaattc cggatcgtat ttataaacgg tcgcgccgtc ggaagtgcaa ccgacttgcg
     1741 aaggggagca tatggtgttg agcagttgag ctccgccgcc gacgatgttt tttatgatgg
     1801 acgggcggtc gatggcgtgg gcgatggctt tacgcacgcg ttcatccttc agttcggggg
     1861 ctggcccgcc atcgcgactg ttcaattgca ggaagacgat gcgcatcgtg tttccgctgg
     1921 agacatgcag atttggcatg gacttcagtt gttcggcctg atccttggcc acattcatga
     1981 tgaagtcttc gccgcccgaa ataacttccg ccacctgcgt ttgtttgtcg ggaatgaatc
     2041 gaatgacgac tttgccgatt ttaggttggg ctttgggcga gtccttgaaa taatccttat
     2101 tgcgttccag cgtgattgat ttgccgggtt ggtatgccgt cactttgtaa gggcccgttc
     2161 cgacgggctt ggcgctcatg ccagccgggc cgacttcctt gtaatacttg gcgggcatga
     2221 ttgccaccgt ggtcgacagg tattccttgg cggcgggaaa tggctttttg gttataagcc
     2281 tgactttgta tttgcccagc ttttcggcct tgtcgatcca actgacattc gcttgtgtcg
     2341 ttgccttgtt ttttggatcg gcaacgaagt tcagtgtgta gacgacagag tcggcgtcga
     2401 attcttcacc attgtggaat ttgatgcctt cccgcaaatc gaaatcgatg gttttgtcgt
     2461 ccacctgctt ccagctcttt gccagttgcc ctttgtattc gttggtaagc ggatcgcgat
     2521 atagcagtgt gtcccagacg ttggcggcga taattacgcc gatgcgcacg ttattgaaat
     2581 aaggatcgac actttccggc gcctgatcgt aggccatacg caaagtgtcg tctttttttg
     2641 tcgccagagc ggaagtgctg acgcacagaa agcttgccat cagcaggctc cccgccagcg
     2701 tcttgaaaga gaatttatgg ggaagtgcgg ctatcaggct gcttggcttc tcagtcatgg
     2761 gcttgtctcc tttttgaccg tgtgttgacc tgccatcaag gcaggccttt tttttggttt
     2821 cgcggttagt gtaaattaaa tcatgccaat gcgcgctcca aaacccgggc gacgtgttcg
     2881 gccttgcgcg ccacgccatc gcgtgtgcca tcggcgatct gatggcggca gcttgttccg
     2941 tcggccacta ataacgtgtt ggcgtcggct ttgcgtattg cgggcaataa cgatagttcc
     3001 gccattttca tcgaaatgtc atagtgtttg gcttcgtagc caaagctgcc ggccatgccg
     3061 cagcagctcg attcaataag cgagacatca agctcgggga tgaggcccag caccacctgg
     3121 accggtttga cagcgtcaaa ggccttttgg tgacagtggc cgtggagcag ggcacgtttt
     3181 tcgggaagcg ccttaagctt caggtgcagc gttcccgctt tttgttcgcg cgccaggaat
     3241 tcctcaaaca agaacgaatt tttggccagg gtagtggcgt ctgcgcccat gcccatcacc
     3301 aggaattcgt cgcgcaacga tagtaagcag gacggttcca gcccgaccac gggaatcccg
     3361 cgcgtaacga acggccgtag cgcggccacg acgcgtttgg cttccagctt ggcttcgtcc
     3421 accagcccgg cggataaata cgtgcgcccg cagcatagtg gacgattggc gtgggcgtcg
     3481 tttttcaacg ggcgcgcgat atggacgcgg tagcccgccg cctgcaacac gttcagcgcc
     3541 gcgcgtgagt tttcagattc caggtagttg ttgaaggtgt cggcaaacaa gaccacgttg
     3601 gcgctggctg cgtcggccga atgggcgtcg cgcaggaaag tgtcgcgccg ccattttggc
     3661 agcgagcgtt tggccgaaaa ccccaaccat ttttccgata tcattttggc gcctggcagg
     3721 gtgtcgcgca ggttgaacag gaaagggaac cgtgctgccc agggagccca gtggggcagc
     3781 gtggcaatca gcctgtcttt cagcttgagc ccgtattttt tttgccattg atacaggaac
     3841 tcgattttca tcttggccat gtcgacgcca gtcgggcatt cgcgccggca tcctttgcag
     3901 cccacgcaca aatccatggt ttcgtgcatt tcgcctgacg ttaacgcgtc gggtccaagt
     3961 tggcccgaca gagccagcct gagcgtgttg gcgcggccgc gcgtcaggtg cttttcgtcg
     4021 cgcgtgacgc gatagcttgg gcacattgtg cccgtatcga acttgcggca atggccgttg
     4081 ttgttgcaca tttcgatggc cttgccgaag ccgcccgccg catcgccgcc cgtgccgggc
     4141 gcgctggtgg tctgcttgtc cgggtcgttt tgcacgttcc acgccgacca gtcgaacgcg
     4201 ggcctcatgg gcaatacctg gtaggtaggc ttgaagcgaa agagcgatgt gttgtccatt
     4261 ttcggacagc gaacgatttt gcccggattc atcatgcctt ccggatcgaa caggtcttta
     4321 atttcttcga acgcgtgggt cagccgaggc ccgaactgcc aggatatcca ttcgctacgc
     4381 gccaggccgt cgccgtgttc tcctgaaaag gcgcctttgt atttgcgcac catgtcgctc
     4441 gcttcttcgg cgatggcgcg cattttctct gcgccgctgg tgcgcatgtc caggatcggc
     4501 cgaacgtgta aagtgcccac cgaggcgtgg gcgtaccagg tgccgcgcgt gccgtgcttg
     4561 cgaaaaattt cagtcaggcg gttggtgtat tcagccaggt gctccagcgg cacggcgcag
     4621 tcttcgatga acgaaacggg cttgccgtcg ccatgcatgc tcatcatgat gttcagtccc
     4681 gccttgcgca cggcccatag ggctttttgc gccgcgggat tgggcatttc aaccacgctg
     4741 tcaggcaggc ccagttcgcc cattagttcc accagcttcg ccagtttgcg caattgttcg
     4801 ctgttgtcct cgcccgcgaa ttcaaccagc aggatggctt cgggttcacc gatgaccgct
     4861 ttatcgataa ccggcttgaa cgccgggttt tcgcgcgcca gatcgatcat ggtgcgatcc
     4921 accagttcga cggcgcaggg tcccagcttg actatgtgct gcgccatgtc catggcctgg
     4981 tagaacgtcg ggaagttcac cacgccaagc gctttgtggg ccggcaaggc agagagcttt
     5041 aaagttattt gctgcgtgta ggccagagtg ccttcgcttc ccaccagcaa atgcgccaag
     5101 ttgacgcttc catcgctggt gtagggccgc tcgctttgcg ggttgaaa
//
LOCUS       DATCXU010000436         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_283638,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000436 DATCXU010000000
VERSION     DATCXU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283638"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VIR04_09785"
     CDS             <1..1168
                     /locus_tag="VIR04_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY9461082.1"
                     /translation="CGGPARRETDTMDTFVDSSWYFMRYTSPGNDKAMVDARNDYWMP
                     MDQYIGGIEHAVLHLLYARFWTRVMRDLGLVKFDEPFTKLLCQGMVLNHIYSRKNEHG
                     AIDYFWPDEVENIVDSKGAIVGAKRISDGSVVDYNGIGTMSKSKNNGVDPQSLINSMG
                     ADTARLFVMFASPPEQTLEWSDSGVEGANRFLRRLWSFCHTRQAAVLGAAPAPNVDGS
                     TDVEALKTLRRDIHSLLKQADYDYQRIQYNTVVSTCMKMLNTIESALLPDTARGRQVL
                     AEVLSILLRVLYPVVPHITWRLWQDLEFDKAYGDLLDAAWPEVDQAALIADEIDLVLQ
                     VNGKLRGTLKVSHDAARAEIEKLAARHDAIARFLEGRPVKRVIVVPGKLVNIVG"
     gene            1177..1836
                     /gene="lptE"
                     /locus_tag="VIR04_09790"
     CDS             1177..1836
                     /gene="lptE"
                     /locus_tag="VIR04_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY9461083.1"
                     /translation="MRRLPLKQSPPFSAFARPARAWACIMLCVLLGACGFKLRGVSPL
                     PFNTMYTNIPENSDFGSRLRRAVIASSPHTRFVTDAAAADAKLIQLSNTQSLREVSIN
                     AQGLVEEYELNLAFVFQLTDAKGHVLLAPTTLTATREIPYDATAVQAKQGEIGNLFIE
                     MQQSLIDRVIRRLTSPDVIEAVKNAESGPVEANPADITPIAPQARPPMPLSNAPGAGS
                     H"
     gene            1843..2946
                     /gene="holA"
                     /locus_tag="VIR04_09795"
     CDS             1843..2946
                     /gene="holA"
                     /locus_tag="VIR04_09795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY9461084.1"
                     /translation="MGRRLDSNRLASDLAQARRPPDPLYIVSGDEPLLMTEAIDALRA
                     AAARAGYTERISLALDARSDWSAVTAATQNVSLFGDRRLIELKIPSGKPGKTGSNVLI
                     KLAGLILEGVLTDVLILISLPRLDRATRGSKWAMSLDRAGTSVDVPTIERAALPGWIA
                     QRLTRQRQQLDPDSLQWMADKVEGNLLAAFQEIQKLALLYPEGMIRAADVERAVLNVA
                     RYNVFDLRDAMLAGDAARALAILTGLRAEGEALPLVLWAVGEEIRIMARVAWAQALGR
                     DLSGELRAQRVFGVREQLMRQTLARLPARSWPDAVRHSHEVDRLIKGLPTPGRLDDPW
                     EELARLILRIAVPRKHAAAKSAIIQSSTIPHST"
     gene            3000..4238
                     /locus_tag="VIR04_09800"
     CDS             3000..4238
                     /locus_tag="VIR04_09800"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY9461085.1"
                     /translation="MSILGVRARRASRVMMTASSQAKATALRAIASCLQEQKAQLQAE
                     NAKDLAEAQKNRLAPALVDRLTLSDQAVQKMAAGLLQIADMPDPIGSLTASNIRPNGM
                     QVAQMRVPLGVIGIIYESRPNVTIDAAALCLKSGNAAILRGGSEAFHSNQALGAIVKK
                     GLTQAGLPADAVQVVNTTDRDAVGQLITMTEYIDVIIPRGGKGLIARLSAEAKVPLIK
                     HLDGNCHVYIDAAADLDKGHDIAVNAKTYRFGVCGAMETLLVHRDIAPKILPRLAQTL
                     VAKGVELRGCDRTCALIGNAAPATEEDWATEYLGPILAIRIVDSLDQAIEHIARWSSG
                     HTEAIVTENLHNATRFQREVDSSSVYVNLPTCFADGYEYGLGAEIGISTNRLHARGPV
                     GLEGLTTLKWVLMGDGQLRG"
     gene            4276..4692
                     /locus_tag="VIR04_09805"
     CDS             4276..4692
                     /locus_tag="VIR04_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY9461086.1"
                     /translation="MLWIKTLHLLFVISWFGGLFYLPRLFVNMAQRHDAATIECLRDM
                     AVRLYKFMSVLAILAIIFGLWLFLGYRIGQGQGWMHAKLGLVIVLVGYHLFCGRLLKK
                     FEQNQNRRSERFYRWFNEIPVILLLMILTLVIVRPF"
     gene            4705..>5619
                     /locus_tag="VIR04_09810"
     CDS             4705..>5619
                     /locus_tag="VIR04_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY9461087.1"
                     /translation="MNDPQNPRKKLGLKKATTGTPSGPALDRRKRSGGRARQIAQIER
                     ARDSDPRNRRVGPHTPASGAAEPNPSNLNPSTSRNPAPHPRASLPRAIAPAEIFPIFT
                     PCPQGLEEALAAELQTLGFEDVRLARAGCSFHADWTGIQRANLYSRLATRVLVQVAQG
                     PALTEDDIFDLAYSTEWERWFGAEQTLRVDTSAIRSPMQSLQFCNLRAKDGICDRLRD
                     REGARPDIDTVRPDARVHLFLTEDSATLYLDTSGESLFKRGWRLDKGTAPIRENLAAG
                     LLALSGWDPSQALLDPFCGSGTILIEAAG"
BASE COUNT         1189 a         1749 c         1585 g         1096 t
ORIGIN      
        1 gtgcggcggg cccgcccggc gtgaaaccga caccatggac acgtttgtgg attcgtcctg
       61 gtatttcatg cgctatactt cgcccggcaa tgacaaggcc atggtcgacg cacgcaacga
      121 ttactggatg cccatggatc agtatattgg cggcatcgag catgcggtgc ttcacctgct
      181 ttatgcacgg ttctggacgc gcgtcatgcg tgacctgggt ctggtcaaat tcgatgagcc
      241 gtttaccaaa ctgctgtgcc agggcatggt gctgaatcat atttactccc gtaaaaatga
      301 gcatggggcc attgattatt tttggcccga cgaagttgaa aacattgtcg attcgaaagg
      361 ggccatcgta ggtgcaaaac gtatctccga tggttcagtc gttgactaca acggcatcgg
      421 caccatgtcg aaatccaaaa ataatggcgt cgatcctcag tcgctgatca attccatggg
      481 cgctgacacc gcccgcttgt tcgtcatgtt cgcaagccct cccgaacaaa cgcttgaatg
      541 gtcggactcg ggcgtcgagg gggccaatcg attcttgcgc cgtctctggt cgttttgcca
      601 tacccggcaa gcggcagtgc tgggggctgc gcccgcaccg aatgtcgatg gatccactga
      661 tgtggaagcc ctcaaaacgc tgcggcgcga catccacagt ctgctcaaac aggccgacta
      721 cgattaccag cgcattcagt acaacaccgt cgtctccacc tgcatgaaaa tgctcaacac
      781 gatagagtcc gccttgctgc ccgataccgc gcgagggcgt caggtcctgg ccgaagtgct
      841 gagcatattg ttacgcgtgc tctatcccgt cgtaccgcac attacctggc ggttgtggca
      901 agatcttgaa ttcgacaaag catatggcga cctgctcgac gccgcgtggc ccgaggtaga
      961 ccaggcagcc ctgattgccg atgaaatcga tctggtgctg caagttaacg gcaaattgcg
     1021 cggcacgcta aaagtttccc acgatgccgc ccgcgccgaa atcgaaaaac tggccgctcg
     1081 ccacgatgcc attgcccggt ttcttgaagg ccggccagtg aagcgagtca tcgtcgtgcc
     1141 cggaaaatta gtgaatattg tcggatgaaa tcagccatgc gccgcctccc actcaaacaa
     1201 tcgcccccct tctcagcgtt cgcgcgaccc gcgcgcgcat gggcctgcat tatgctgtgt
     1261 gtgttgctgg gcgcctgtgg tttcaagcta aggggcgtgt cgccactgcc ctttaacacg
     1321 atgtacacca atatccccga aaactcggac ttcggttcgc gcctgcgccg cgccgtgata
     1381 gccagttcgc cccatacccg tttcgtgacg gacgcggccg cggccgatgc caagcttatt
     1441 caactgtcca atacccaatc tttacgcgaa gtatccatta atgcgcaagg gctggtcgaa
     1501 gaatatgaac ttaacctggc ctttgtcttt cagcttaccg atgccaaagg ccatgttctg
     1561 ctagcgccga cgacacttac cgccacccgc gagataccct acgatgccac cgccgtgcag
     1621 gccaaacagg gcgaaatcgg caatctgttc attgaaatgc agcaaagtct gattgaccgc
     1681 gtcatacggc gccttacctc gccggacgtc atcgaagctg tcaagaatgc cgaatcagga
     1741 cctgtcgaag ccaaccccgc cgatatcacg ccaatcgcac cccaggccag gccgccgatg
     1801 cccttaagca acgcgcctgg ggccggatcg cactgacaga ccatgggacg ccggcttgat
     1861 tccaaccgcc tcgcaagcga tctggcgcag gcccgacgac cgcccgatcc cctatacatc
     1921 gtttccggag acgagccttt acttatgacc gaggcgatcg acgcactgcg ggcggccgct
     1981 gcgcgcgcgg gctacaccga acgcatcagc ctggcgctcg acgcgcgcag cgactggtca
     2041 gcggtcacgg cggcaacgca aaatgtttcg ctattcggcg accgccgcct gatcgaactg
     2101 aaaataccct ctggcaaacc gggcaaaaca ggcagcaacg tgctcattaa gctggccggg
     2161 ctgatccttg agggcgtcct gaccgatgtc ctgatcctga taagcctgcc acgtctggac
     2221 agagccaccc gcggcagcaa gtgggccatg tcgctggatc gggcaggaac aagcgttgat
     2281 gtccccacta tcgagcgcgc cgccctgccc ggctggatcg cgcaacgact gacacggcag
     2341 cgacaacagc ttgaccccga cagcctgcaa tggatggcgg acaaggtcga gggcaatttg
     2401 ctggcagcct ttcaggaaat tcaaaagctg gcgctgctgt atcccgaagg gatgatcagg
     2461 gccgccgatg tcgaacgcgc tgtgttgaat gtcgctcgct ataatgtatt cgacttgcgc
     2521 gatgccatgc tggccggcga cgctgccagg gccctggcca tactgacggg gttgcgcgct
     2581 gaaggcgagg cgctgcccct cgtgctgtgg gcggtcggcg aggaaatccg cattatggcc
     2641 cgggtggcct gggcgcaggc gctggggcgg gatttatccg gcgaattgcg cgcgcaacgt
     2701 gtatttggcg tgcgcgagca acttatgcgt caaacactgg cgcgcctgcc ggcccgaagc
     2761 tggcccgacg ctgtacgaca ctcgcacgag gtcgatcgcc tgattaaagg cttgccgacc
     2821 cccggacggc tggatgatcc atgggaagaa ctggcacggt taatcctgcg cattgccgta
     2881 cctcgaaaac atgccgccgc gaagtcggcg ataattcagt caagcacaat accgcactca
     2941 acatgaccca acccactcat cgaaccgcaa ccgcctcaat tgtctccaag ccctccgaca
     3001 tgagcatcct gggtgtgcgg gcgcggcggg cttcacgcgt aatgatgact gcatcaagcc
     3061 aggccaaggc gacggcgcta cgcgccatcg cttcgtgcct gcaagagcaa aaagcgcaat
     3121 tgcaggcaga aaatgccaaa gaccttgccg aagcgcaaaa aaaccggttg gcgccggcac
     3181 ttgtcgatcg cctgacgctg tcggatcaag cggtgcaaaa aatggcggcc ggcctgcttc
     3241 aaattgccga tatgcccgac cccataggca gcctgaccgc aagcaacata cgtcctaatg
     3301 gcatgcaagt ggctcaaatg cgcgtgcccc tgggcgttat cgggattatt tacgaatcaa
     3361 ggccaaacgt caccatcgac gcggccgcac tttgtctgaa gtcgggtaat gccgctatct
     3421 tgcgcggcgg cagcgaggcg tttcattcga accaggcgct gggggccatc gtcaagaagg
     3481 gcctgaccca ggcagggctg cccgccgacg cggtgcaggt ggtgaacacg accgatcgcg
     3541 atgccgtcgg acagctcatt acaatgaccg agtacatcga cgtcatcatt cctcgcggag
     3601 gcaaggggct tatcgccaga ctgtcggccg aagccaaggt gccgctcatc aaacatctgg
     3661 acggaaattg tcacgtttac attgacgcag cggccgatct ggacaagggt cacgatattg
     3721 ccgtcaacgc caaaacatac cggttcggcg tgtgcggagc catggagacc ctgctggttc
     3781 accgcgacat cgcgcccaaa atacttcccc ggctggctca gacacttgtt gccaaaggcg
     3841 tcgaactccg tggctgcgac cgcacgtgcg cgctcattgg caacgcggcg ccggccaccg
     3901 aagaagattg ggccaccgag taccttggcc ccatcctggc gattcgaatc gttgacagcc
     3961 tggatcaggc aatcgaacac atcgcgcgct ggagttcggg ccataccgaa gcaatcgtca
     4021 ccgaaaatct gcataacgcc acacggtttc agcgcgaggt cgactccagt tccgtctacg
     4081 tcaacctgcc cacctgtttt gccgacggct acgaatacgg cctgggcgcg gaaatcggta
     4141 tttcgaccaa ccgcctgcat gcgcgcggtc cagtggggct ggaaggcctt accaccctga
     4201 aatgggtgct tatgggcgac ggccaactgc gcggttgaat aaccaccgct ttcttataaa
     4261 cggatatggc cctgcatgct ctggattaaa acacttcatc ttttattcgt catttcatgg
     4321 ttcggcggcc ttttctacct gcctcgcctg tttgtcaata tggcgcaacg ccacgatgcc
     4381 gccacaatag aatgcctgcg cgatatggcc gtgcgtttgt acaagttcat gagcgttctg
     4441 gccattttgg caattatttt cggactatgg ctattcctgg gctaccgcat tggccagggc
     4501 cagggctgga tgcacgccaa actcgggctg gttatcgtgc tcgtcggcta ccatcttttc
     4561 tgcggccgct tgctgaagaa attcgaacaa aaccaaaacc ggcgcagcga gcgcttttac
     4621 cgctggttca acgaaattcc agtcattttg ctactcatga tcctgacgct ggtcattgtg
     4681 cggcctttct gaatctgaag aaccatgaac gacccgcaaa acccacgaaa aaaactcggc
     4741 ctgaagaagg caacaacagg gacgccctcc gggcccgcac tggatcgccg caaacgctcg
     4801 gggggccgcg cccggcagat cgcacagatc gaacgcgccc gcgattcaga tcctcgcaac
     4861 cgccgagtcg ggccgcacac gcccgcgtcc ggcgcggcag agccgaatcc atcaaatctg
     4921 aatccatcca cgtcccgtaa tcctgcgccg cacccgcgcg cgtcgctccc acgtgccatc
     4981 gcgccagcgg agatctttcc gatttttacc ccctgtcctc agggtctgga ggaagcgctt
     5041 gccgcggaac tgcagaccct ggggtttgaa gatgtccggc tcgcgcgagc cggctgcagt
     5101 ttccacgctg actggaccgg catacaacgc gccaacttgt attcccgcct ggccactcgc
     5161 gtcctggtgc aggttgccca gggcccggcg ctaaccgagg atgatatttt cgatctggcc
     5221 tacagcaccg aatgggaacg ctggttcggc gcggagcaga cgctgcgggt cgatacctcg
     5281 gccatccgca gtcccatgca aagcctgcaa ttctgcaacc tgcgtgccaa agatggcatt
     5341 tgcgaccgcc tgcgcgatcg cgaaggggcc cgccccgata tcgacaccgt gcgcccggat
     5401 gcgcgtgtgc acctttttct gactgaagac agcgcaaccc tgtacctcga cacatcgggc
     5461 gagtcgctct tcaaacgtgg ctggcggctc gacaaaggaa ccgcccccat acgcgaaaac
     5521 ctggcggcgg gcttgctggc cctgtccggc tgggatccat cgcaagccct gctggaccca
     5581 ttctgcggta gcggcactat tttaatcgaa gcagcgggg
//
LOCUS       DATCXU010000437         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_518610,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000437 DATCXU010000000
VERSION     DATCXU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518610"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VIR04_09815"
     CDS             <1..1145
                     /locus_tag="VIR04_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY9461088.1"
                     /translation="ALYHDIAKGRGGHHSELGARDVHRFCRDHAIDRADTELVEFLVR
                     HHLTMSNFAQKRDLTDPTVVHDFAHCVKDERHLTGLYLLTVADIRGTSPKVWNSWKGK
                     LLEDLYHLTLVALGGSQPDANTLLAQRKQAAAAEIRLMGLRDESRDKLWEKLDVAYFL
                     RHDAGDIAWHTRHLYYRSESPDPVVKVRLIGLSDGLQIMVYTRDRDDLFVTICRYFYR
                     RGLRIMDARIHTTLHGWALDSFVVLMPAHEDVRSNATLVEHELTEKLKQDGAAEPAPA
                     SDPMRRPGSRRARVFPIVPNVELQPDESGASWRLSVTAADQAGLLYSLSQVFATYSIN
                     LKMAKVMTLGDRVEDVFIIEGDVLGQPRAQLQFERSVLSRLDQSNQ"
     gene            1171..1872
                     /locus_tag="VIR04_09820"
     CDS             1171..1872
                     /locus_tag="VIR04_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY9461089.1"
                     /translation="MIINDLLANFGRSFLFALATLLPILNPPAVAPIFLTLTEGASAQ
                     SRSALARRVSINIFIMLAIAIIAGNLVLDFFGISLPIVRVGGGLLVIATAWQLVSASD
                     SDTRHAQNMAEMYTPEQVSAQAFYPLTFPMICGPGSLAAALTVGATLHGESISDTLST
                     LTGSIFAILAASLVVYFVLRFAAQLLYKLGRNGTAVLMRLSAFILLCLGVQIVWDGAH
                     ELLLGLLREAAAHRY"
     gene            1916..2395
                     /locus_tag="VIR04_09825"
     CDS             1916..2395
                     /locus_tag="VIR04_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY9461090.1"
                     /translation="MSNTTSRLLNLIALLSIGAVGIALISQYVFGLQPCAWCVLQRLI
                     YLLIAAFCWLGLLLNRLPALRKTCALLAALLSVGGAAAAWYQYDVAAQLFSCKLTFAD
                     RFMVGAGLDAHVPWLFGILASCADAKARILGVEYALWSLALFAFLGVLSLVALLRRR"
     gene            complement(2458..3954)
                     /gene="purF"
                     /locus_tag="VIR04_09830"
     CDS             complement(2458..3954)
                     /gene="purF"
                     /locus_tag="VIR04_09830"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY9461091.1"
                     /translation="MCGLVGVVGPIPVNQLIYDSLLLLQHRGQDAAGIATLHDQFFYM
                     HKGHGLVRDVFRTRNMRSLLGFSGLGQVRYPTAGSSHNVAEAQPFYVNAPFGITFIHN
                     GNLTNWRELREALFKIDRRHINTNSDSEVLLNVFAHELQQSASGVSLDENAIFSAVSA
                     VHRRAKGAYAVIAEIASCGLVAFRDPFGIRPLCLGTLETESGTQWMVASESVALTGCG
                     FTFIRDVAPGEAVVIDFDGNLTSRQCAEKPVLTPCVFEYVYFARPDSMMNGVSVYDAR
                     LGMGEYLADNVAKTLRLGEIDVVMPIPDSSRPSAMQLAARLNLNYREGFIKNRYVGRT
                     FIMPGQTVRKKSVRQKLNAIDMEFKGKNVLLVDDSIVRGTTSREIVDMARAAGAHRVF
                     FASAAPPVRFPNVYGIDMPTQAELIAAGRNADQVAHEIGADGLVYQDLDDLQQAIRRL
                     NPTLKDFESSCFNGHYVTGDIDAAYIERLSQTRGEQAKMTSTVDEEDA"
     gene            complement(3977..4465)
                     /locus_tag="VIR04_09835"
     CDS             complement(3977..4465)
                     /locus_tag="VIR04_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY9461092.1"
                     /translation="MTSFDYIVLAILGVSAFLGLLRGLVKEILSLIAYVVAFVATIWW
                     GPNMSTWIARFIDNGLLRTAVAYAAVFIIVLLLVGLVNITLGTLVRKSGLTPADHGLG
                     ALFGFSRGLLIVLVLVGLAGYTQLPKEPWWRDAQLSAPAVSAVQRIKALLPPSLASWL
                     PY"
     gene            complement(4462..5241)
                     /locus_tag="VIR04_09840"
     CDS             complement(4462..5241)
                     /locus_tag="VIR04_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY9461093.1"
                     /translation="MSSEAQARELRGRARRRLIGALALVVAAVIVVPMLLDTEPTPDQ
                     AKAPLVVPAITPPAPQTGAPTAVPGDADSTADADAPLPTPDEALPDSGVSGDAAAPPA
                     EVPPPPAKPRSESAPSARPEASTAPQAKSRPSEKSSESRAEAKSESKSNHVKRTDDGS
                     AALALLEGRQPRQASPEKKPAQGNYTLQIAAYSADKDAQARRDRLVASGVTNAYIETA
                     TSGGKATYRLRVGPFPTREAAQAAQARLRALGYDNGFISTK"
     gene            complement(5365..>5927)
                     /locus_tag="VIR04_09845"
     CDS             complement(5365..>5927)
                     /locus_tag="VIR04_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY9461094.1"
                     /translation="AVRLGLQMSLPGRLQILPGHPTIVLDVAHNPHAAAALGQNLDGM
                     SYFPQTHAVFGMLRDKDIAGVVAKMASRVDHWYCATLEGPRGVEGGHLAEIVRAALPT
                     GPKKTMVSTSAIRIKAPNRIPAVAARRSPIPVKEKPPVTVASFDDPVQAFAEAQKQAS
                     GDDRIVVFGSFKTVGPVLKDLGRKVN"
BASE COUNT         1264 a         1776 c         1651 g         1236 t
ORIGIN      
        1 ccgcgctcta tcacgacatc gccaaaggcc gcggcggcca ccactcggaa cttggcgcgc
       61 gcgatgttca ccgcttctgc cgcgatcacg ctatcgaccg cgccgatacc gaactcgtcg
      121 agtttctggt gcgccatcac ctgaccatgt ccaactttgc gcaaaagcgc gacctgaccg
      181 acccgaccgt tgttcacgac ttcgcgcatt gcgtgaaaga tgagcgccat ctgaccggac
      241 tatatctgtt gaccgtcgcc gacattcgcg gaacgagccc caaggtctgg aattcatgga
      301 aaggcaaact gctggaagac ctgtatcacc tgacgctcgt ggccttggga ggctctcagc
      361 ccgatgcaaa taccctgttg gctcaacgca aacaagcggc agccgccgag atccggctta
      421 tgggcttgcg cgacgaaagc cgcgataaac tttgggaaaa actcgacgtc gcctattttt
      481 tgcgtcatga tgccggcgac attgcatggc atacgcgtca tctgtactac cgctcggagt
      541 cgcccgatcc ggtcgtgaag gtacgactga tcggtctcag cgatgggctg caaatcatgg
      601 tctacacccg cgacagggat gatctgtttg tgaccatttg ccgctatttt taccgccgcg
      661 gtttgcgcat tatggacgcc cgcatccaca ccacgcttca cggctgggcg ctggacagtt
      721 tcgtcgtgct gatgcccgcg cacgaagatg tccgctccaa cgccacgctg gtagagcacg
      781 agctgactga gaagctgaaa caggacggtg cagcggaacc cgccccggca agcgacccaa
      841 tgcggcggcc cgggtcgcgg cgagcgcgcg tatttcccat tgtgcccaac gtggagttgc
      901 aacccgacga aagcggcgcc tcgtggcgtc tgtccgttac tgcagccgat caggccggat
      961 tgctttatag tctgtcccag gtattcgcca cgtattccat caacttgaag atggccaagg
     1021 tcatgacgct gggagaccgg gttgaagacg ttttcattat agaaggggat gtattgggcc
     1081 agccgcgggc tcaattgcaa tttgaacgca gtgttttgag ccggctggat caatcaaatc
     1141 aataacaggc catgcgcacg caagaaaccc atgatcatca atgacctttt ggcaaatttc
     1201 gggcgcagct ttttgttcgc gctggccacc ctgttgccca ttctgaaccc gcccgccgtg
     1261 gcgcccatat tcctgacttt gaccgaaggc gccagcgcac aatcgcggtc tgcccttgcg
     1321 cggcgcgttt cgatcaacat cttcatcatg ctggccatag cgataatcgc gggcaatctg
     1381 gtgctcgact tttttggcat ttccctgccg atcgtccggg tgggcggcgg tctgctcgtc
     1441 attgccaccg cctggcaact ggtcagtgcg tcggattccg acaccaggca tgcgcaaaac
     1501 atggccgaaa tgtatacgcc ggagcaagtc agcgcccaag ccttctaccc attgacgttt
     1561 cccatgatat gcgggcccgg gtcgctcgcg gccgcgctga cggtgggcgc cactttgcac
     1621 ggcgaatcga tttcagacac cttgtctact ctgacaggat cgattttcgc catactggcc
     1681 gcctctttgg tggtttactt cgtcctgcgc ttcgcggcgc aactcttgta caagctcgga
     1741 cgaaatggaa cggcggtcct catgcgcttg tcagccttca tcctgctctg ccttggagtg
     1801 caaatcgtat gggacggagc gcatgaatta ctacttggcc tattgcggga agccgcggca
     1861 catcggtact gatacggcag caatagtcta ttttttcaac ttcaggtcac agattatgtc
     1921 caacaccacc tcccggctgc ttaatctgat cgcgctgctg tccattggcg cggtcgggat
     1981 tgctttaatt tcgcaatacg tgtttggctt gcagccctgt gcgtggtgcg tcttgcaacg
     2041 gctgatctac cttttgattg ccgcgttctg ctggcttgga ctgctgctga accggctacc
     2101 cgcactgaga aaaacatgtg cgctgttggc ggcccttcta agcgtgggcg gagcagcagc
     2161 ggcctggtat caatacgacg tcgccgcgca actattttca tgcaagctta cttttgccga
     2221 tcgctttatg gtcggcgccg gcctggatgc tcatgtgccc tggttgtttg gaatacttgc
     2281 ctcgtgtgcg gacgcaaagg cacggattct aggcgtggaa tacgcattat ggagcctggc
     2341 tcttttcgct tttctcggcg tattgagcct tgtcgctttg ctgcggcgcc gataggccgc
     2401 caccccatca aaatgcccgg cgcggcaggc gcctgcatta ctgccggctt gcgacgttca
     2461 agcgtcctcc tcgtcgacgg tcgaagtcat tttcgcctgc tctccgcgag tctggctcag
     2521 tcgttcaata tacgcggcat caatatcgcc agtcacataa tgcccattga agcaggacga
     2581 ctcaaagtcc ttcaaagtcg gattcaaccg ccgaatcgct tgttgcagat catccagatc
     2641 ctggtaaacc agcccatcag cgccaatctc gtgggcgacc tgatccgcgt tccgacccgc
     2701 cgcaatcaat tcggcctggg tgggcatatc gataccgtaa acgttgggga aacgcacagg
     2761 tggcgcggct gaagcaaaga aaacccggtg ggctccggca gcgcgcgcca tatcgacgat
     2821 ttcgcggctc gtggtgcctc tgacgatcga atcgtcaaca agcagtacgt ttttgccctt
     2881 gaattccatg tcgatcgcat tgagcttttg ccgcaccgat ttttttcgca ccgtttgccc
     2941 cggcatgata aaggttcggc caacatagcg gttcttgata aacccttcgc ggtaattcaa
     3001 attaagccgc gcggccagtt gcatcgccga aggacgggac gaatcgggaa taggcatgac
     3061 cacatcgatt tcgcccagac gcaatgtctt ggcgacattg tcggccaaat actcgcccat
     3121 gcccaggcgc gcatcgtaaa ccgacacgcc gttcatcatg gagtcggggc gcgcgaagta
     3181 aacgtattcg aacacgcacg gggtcagcac aggcttttcc gcgcattggc ggctggtcag
     3241 attgccgtcg aaatcgataa caacagcctc tcccggagcg acatcgcgaa taaacgtgaa
     3301 accgcaaccc gtcaaagcaa cagactcgga ggccaccatc cactgcgtgc cgctttcagt
     3361 ttcgagcgtc cccaaacaca gcggccgaat tccaaaagga tcccgaaagg caacaagacc
     3421 gcaactggca atctccgcaa tgaccgcgta agcgcctttg gcgcgccggt ggaccgcgga
     3481 cactgccgag aaaatggcgt tttcgtccag cgaaaccccg ctggccgact gctgcaactc
     3541 gtgggcgaac acgttcagca ggacctcgga atccgaattg gtattgatat ggcgccgatc
     3601 tattttaaat aatgcctctc gcagttcgcg ccagttggtc agattgccgt tatgaatgaa
     3661 agtgatgccg aagggcgcat tgacataaaa gggttgagcc tccgccacgt tgtggcttga
     3721 tcccgcagtt ggataacgca cctgccccag gccgctgaag cccaacaatg aacgcatgtt
     3781 tcgcgttcga aacacgtcac gcaccagccc gtgcccttta tgcatgtaga aaaattgatc
     3841 gtgcagcgtc gcaatgcccg ccgcgtcctg accgcgatgc tgcaacaaca ataagctgtc
     3901 atagatcaac tggttcacgg ggatcggacc aacaacccct acgagtccac acatttggat
     3961 gttcctgtta aaacaatcaa taaggcaacc aggatgccag cgacggagga agcaaagctt
     4021 tgatcctctg tacggcggaa acggcaggcg cggacaactg cgcatcgcgc caccatggct
     4081 cttttggcaa ctgcgtgtat cccgcaaggc ccacgagcac cagaacgatc aacaaccccc
     4141 ggctgaaacc aaacaaggcc cccaagccat gatcggccgg agtaaggcct gactttctta
     4201 ccaaagtgcc caaggtgatg ttgaccagcc caaccagcag aagaacgata atgaataccg
     4261 cggcataagc gacggccgtt cgcaacaaac cgttatcgat aaaacgcgcg atccaggtcg
     4321 acatgttcgg cccccaccag atcgtggcta caaaggcgac aacgtacgca atcagcgaaa
     4381 gaatctcttt tacaaggccc ctgagcaggc ccagaaatgc cgaaacgccc aaaatcgcca
     4441 ggacaatgta atcgaagctg gtcacttagt ggaaataaaa ccgttgtcgt agcccaaggc
     4501 gcgcaagcgg gcctgagccg cctgagccgc ctcacgcgtg gggaacggtc ccacgcgcaa
     4561 tcgatacgtt gccttcccgc cggacgtcgc ggtttcgata taggcgttcg ttacgccaga
     4621 tgccaccagt cgatcccgac gcgcctgagc gtctttatcg gcggagtaag cagctatctg
     4681 cagggtgtaa ttaccttgag ccggcttctt ttcgggggat gcctggcgcg gttgacgccc
     4741 ttcgagcagc gccagcgccg ccgaaccatc gtcagtgcgc ttcacgtgat ttgatttgga
     4801 ttcggacttg gcctcggccc tggattcgga cgatttttca ctgggccgtg atttggcttg
     4861 aggagcggta cttgcctcgg ggcgcgccga tggcgctgat tcagatcgcg gcttggccgg
     4921 aggcggcggc acctcggccg gtggtgcggc ggcatcgccg gacacgccgg aatcgggcaa
     4981 agcttcatcg ggggtcggca acggcgcatc ggcatcggcc gttgaatcgg catcgcccgg
     5041 cacggcagtg ggcgcgcccg tttgcggtgc gggcggagta attgccggca caaccagggg
     5101 cgccttggcc tgatccggcg tcggttcggt atcgagcagc ataggcacaa caatgactgc
     5161 ggcaacaacc agcgccaaag cgccaatcag acgccggcgc gcacgccccc ggagctccct
     5221 ggcctgggct tcgctggaca cagaagaacg cgatgcggaa cgtcgcccgg aaccgggagc
     5281 agactcgttg cgagagaata atcccataaa ccgtaaaccc ttggtggcga tcaagccagc
     5341 ttgttgtggc ttgtcatgca aacattagtt aactttacgc cccagatcct taagaacggg
     5401 accaaccgtc ttaaaagaac cgaatactac gattctatca tcccccgaag cctgcttttg
     5461 cgcctcggca aaagcttgaa ccggatcgtc aaaacttgca acagtcacgg gaggcttttc
     5521 tttgacggga atagggcttc tgcgagccgc aaccgccgga attctgttcg gcgccttgat
     5581 gcggatcgcg ctggtcgaaa ccatggtctt tttgggaccg gtgggaagcg ccgcgcgaac
     5641 aatctcggcc aagtgtccgc cttcgacgcc gcgcggcccc tccagcgtgg cgcaatacca
     5701 atggtcgact ctgctcgcca tcttggccac cacgccggca atgtctttat cgcgaagcat
     5761 gccgaacacc gcatgggtct gagggaaata agacatgcca tccaggtttt gccccaatgc
     5821 cgccgctgca tgggggttat gcgcgacatc aagcacaatg gtgggatggc ctggcaggat
     5881 ttgcagacgc cctggcaagg acatctgcag cccaaggcgc acggcct
//
LOCUS       DATCXU010000438         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_523145,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000438 DATCXU010000000
VERSION     DATCXU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523145"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VIR04_09850"
     CDS             complement(<1..836)
                     /locus_tag="VIR04_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY9461095.1"
                     /translation="MLNTPFSPWPSFTEEEANALRDVILSNKVNYWTGQECRQFEQEF
                     AAWAQCKYAIALANGTLALDAALKALEIGPGDEVIVTPRTFLASASCVVNAGATPVFA
                     EVDRDSQNITAESARAVLSPHTRAIICVHLAGWPCDMDPIMALAEEHKLWVIEDCAQA
                     HGALYKGRSVGSIGHIGAWSFCQDKIMSTGGEGGMVTTQDRVLWSKMWSFKDHGKSWE
                     AVCNNQHTPGFHWLHETFGSNLRMTEIQAVLGRIQLRRMPQWQASRLTHAQRIWSTAV
                     YLP"
     gene            complement(840..1499)
                     /locus_tag="VIR04_09855"
     CDS             complement(840..1499)
                     /locus_tag="VIR04_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY9461096.1"
                     /translation="MLTALYAVYGASGCGRGIMPLAQLQLQRQGIDVAQLAFVDDKLA
                     GTEVNGRPVLGYAEFINRKASAHYAVLAIANSATRQKLAERCAADGVQPWTVSASNVV
                     TMYDVVIGEGAMLSPFVTLTSNIRIGRYFHANLYSYVEHDCVIGDFVTFAPGAHCNGN
                     IHIQDHAYIGAGAIIKQGTADHPLVIGRGAVVGMGAVVTHNVLPATVVTGNPAKPLIK
                     G"
     gene            complement(1524..2132)
                     /locus_tag="VIR04_09860"
     CDS             complement(1524..2132)
                     /locus_tag="VIR04_09860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY9461097.1"
                     /translation="MVKRLFDLIAATIALIPLSLVMALVAWKVRRQLGSPVLFRQIRP
                     GRNGKPFEIVKFRTMLDIRDQQGRLLSDAERLTPFGRFLRASSLDELPELWNVLNGDM
                     SLVGPRPLLMEYLPLYDQEQRRRHHALPGVTGWAQIHGRNAISWQDKFKLDVWYVDHR
                     TFWLDLKIIALTIKKVLMRDGISAAGEATMPRFTGSPDTSQK"
     gene            complement(2142..>2474)
                     /locus_tag="VIR04_09865"
     CDS             complement(2142..>2474)
                     /locus_tag="VIR04_09865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9461098.1"
                     /translation="SDVRPAIAACSVYVLPSYREGMPRTVLEAMAMGRAIITTDAPGC
                     RETIENGENGCLVPVRSVDALEAAMLRFINEPALAQRMGKRSRRIAESTYDVHKINAV
                     MLNEMGIY"
BASE COUNT          514 a          700 c          712 g          548 t
ORIGIN      
        1 ggcaggtaca cggcggtcga ccagatgcgc tgtgcatggg tcagccgcga tgcctgccat
       61 tgcggcatgc ggcgcaattg aatgcgcccg agaacagctt gtatttccgt catgcgcaag
      121 ttgctgccga aggtttcatg caaccagtga aatccgggcg tgtgctgatt gttgcagacg
      181 gcttcccagc ttttgccgtg gtctttaaat gaccacatct tcgaccataa aacgcgatcc
      241 tgagttgtca ccatgccgcc ttcgccgcca gtgctcataa ttttatcctg gcagaacgac
      301 catgcgccaa tgtggccgat ggaccccacg gatcgtccct tatataaggc tccatgcgct
      361 tgcgcacagt cttcgatcac ccatagttta tgttcttcgg ccagagccat gatcgggtcc
      421 atgtcgcaag gccaaccggc caggtgaacg caaataatgg cgcgtgtatg cgggcttagc
      481 acggctcgcg cggactcggc ggtgatgttt tgcgaatcgc gatccacttc agcgaaaacc
      541 ggtgtggcgc cggcattgac cacgcacgag gcggaggcca gaaaagtgcg cggcgttacg
      601 atgacctcgt cgcccggccc gatctccagc gctttcagcg cggcgtccag ggcaagggtg
      661 ccattggcca gagcgatggc gtacttgcac tgcgcccagg ccgcgaactc ctgttcaaac
      721 tggcggcatt cctgcccggt ccagtaattt actttgtttg acaaaataac gtcgcgcaag
      781 gcgttggcct cttcttcagt aaaagagggc cacggcgaaa atggagtatt cagcatagat
      841 caacctttta tgagaggctt ggcgggattg cctgttacga cggttgcggg caagacgtta
      901 tgcgtcacga cagcgcccat tcccacaacg gcgccgcgtc cgattaccaa cggatggtct
      961 gcggttcctt gtttgataat ggctccggcg ccgatatacg catggtcctg aatgtgaatg
     1021 tttccgttgc agtgagcgcc gggagcaaac gtgacgaagt ctccaatgac gcagtcgtgt
     1081 tcgacatagc tgtacaggtt ggcgtggaaa taacgtccga tgcggatgtt ggatgtcagc
     1141 gtcacaaacg gactaagcat ggcgccttca ccaattacga cgtcatacat ggtgacgaca
     1201 ttggaggcgg acacagtcca cggctgcaca ccgtcggccg cgcaacgctc ggccagcttc
     1261 tgccgtgtgg cgctgttggc gatagccagt acggcatagt gggcgcttgc cttgcggttt
     1321 ataaattcag cgtaaccaag cactggccgg ccgtttacct ccgtcccggc gagcttgtcg
     1381 tcgacgaatg ccaattgcgc cacatcaatg ccttggcgct gcaattgcaa ctgagccagc
     1441 ggcatgatgc cgcgaccgca gccactggcg ccgtatacag cgtagagtgc agtaagcatg
     1501 gttctacttc tataagcgtg agattatttt tgcgatgtgt cgggcgagcc tgtgaagcgc
     1561 ggcatggtag cctcgccggc agcgctaata ccgtctcgca tcaagacttt tttgattgtc
     1621 agtgcaatta ttttcagatc cagccagaaa gtccgatggt cgacatacca gacgtcgagt
     1681 ttgaatttgt cctgccaact gatcgcgttg cggccatgga tctgagccca gccggtaacg
     1741 cccggaagcg cgtggtgacg gcggcgctgt tcctggtcat aaagtggcag atattccata
     1801 agtagcggcc gcggcccaac caggctcatg tcgccattca gcacgttcca aagctcgggc
     1861 aattcgtcca gactgctggc gcgcaagaag cggccgaatg gagttagccg ttcggcatcc
     1921 gacagaagcc ggccttgttg gtcacggatg tcgagcatgg tgcggaactt gactatttcg
     1981 aatggcttcc cattgcgccc cgggcgaatc tgacgaaaca ggacaggtga accaagttgc
     2041 cgccggactt tccatgccac gagcgccata acgaggctaa gcggtatcag tgcaatggtt
     2101 gccgctatca agtcgaacag gcgcttgacc atttctgttc atcagtagat gcccatttcg
     2161 tttaacatta cggcgttgat cttgtgtacg tcatatgtgc tttcggcaat gcggcgtgac
     2221 cgttttccca tgcgttgcgc cagcgcaggc tcattgatga agcgcaacat ggcggcctcc
     2281 agcgcgtcaa ccgatctgac cggaaccaga cagccgtttt caccgttttc tatcgtttcc
     2341 cggcaaccgg gcgcgtcggt ggtgatgatc gcacggccca tcgccatggc ctccagcacc
     2401 gtgcgcggca tgccttcacg gtagctcggc aaaacataga cactacaggc ggcaatggcg
     2461 ggtcgcacat cgct
//
LOCUS       DATCXU010000439         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_548163,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000439 DATCXU010000000
VERSION     DATCXU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548163"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIR04_09870"
     CDS             <1..495
                     /locus_tag="VIR04_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461099.1"
                     /translation="FDPNQPVFAEAESARIGSIDLPKSLLDPLHNGACVNIAANIDER
                     LEFLLQDYGPLFERPEDFKQQLTRLTGLHSKKTILHWHELIDRDARVELFSELIAQHY
                     DPAYARSSHQHFQGIANAMRFTFKPNTENALDQAKALLEQLEHTHSDAGQHPSSAVLP
                     EEQP"
     gene            492..1544
                     /gene="selD"
                     /locus_tag="VIR04_09875"
     CDS             492..1544
                     /gene="selD"
                     /locus_tag="VIR04_09875"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY9461100.1"
                     /translation="MNTIIATPRLTELSHGGGCGCKISPSVLTGLLKTLTPMQAFPNL
                     LVGSETSDDAAVYRLNDEQAIVATTDFFMPIVDDPFDFGQIAAANALSDVYAMGGKPI
                     LALALVGMPINVLPHDVIARVLEGGSSVCARAGIPIAGGHSIDSVEPIYGLAALGVVH
                     PRQVTRNTGAHAGDILILGKALGVGILSAALKKGRLDDAGYRAMIDTTTRLNSVGPDL
                     AALPGVHAMTDVTGFGLLGHTLELCRGAGLTARIRYADLPWIQGALELADAGIHTGAS
                     ARNWASYEDSVELAAHLPAVARTLLADPQTSGGLLVSCAPSAVDAVLALFSAGEFDQA
                     AIIGEMVDGAARIIAE"
     gene            1636..>2036
                     /locus_tag="VIR04_09880"
     CDS             1636..>2036
                     /locus_tag="VIR04_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004551716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY9461101.1"
                     /translation="MESRIDYAAASPDAVKAMMALEGAMRRLGLEQPLVELIKLRASQ
                     INGCAFCVDMHTTDALKAGETERRLNSVVTWREAPFFTARERAALGWTEALTLISETH
                     APDTDYEALRAQFTEAEMVNVTLAIATINCW"
BASE COUNT          436 a          618 c          588 g          394 t
ORIGIN      
        1 tttgacccaa accaacccgt attcgctgaa gccgaaagcg cgcgcattgg gtcgatcgat
       61 ctgccaaaat cgctgctcga tccactgcat aatggcgcct gcgttaacat tgcggccaat
      121 atcgacgaac gcctggagtt cctgctgcag gactatgggc cactattcga acggcctgaa
      181 gacttcaaac agcaactgac ccggctgacg ggtttgcata gtaaaaagac gattctgcac
      241 tggcatgaac tgattgatcg cgacgcacgc gtggaactgt tcagcgagct tatcgcacag
      301 cactatgatc cagcctatgc gcgcagcagc catcagcatt ttcaaggcat tgcgaatgcc
      361 atgcgattca cgtttaaacc caacacggaa aacgcgctgg atcaggcgaa agccctgctg
      421 gagcaactcg aacacaccca tagcgatgcg gggcaacacc cctcgtcggc agtcttacct
      481 gaagaacaac catgaacact atcattgcca cgccacgcct gaccgagcta tcccacggcg
      541 gcggctgcgg ctgcaaaatc tctcccagcg tgctgaccgg gctacttaaa acattaaccc
      601 caatgcaagc tttcccaaac ttgctggtcg ggtcggagac atcggacgac gcagcggttt
      661 accggcttaa cgacgagcag gccatcgtcg caaccaccga ctttttcatg ccgattgtgg
      721 acgacccgtt cgacttcggt caaatagcgg cggccaacgc gctatcagat gtttatgcca
      781 tgggcggcaa gcccatactg gcgctggccc tggtgggcat gcccatcaac gtgcttcccc
      841 atgacgtgat cgcgcgcgtg cttgaaggcg ggtcgtctgt ctgcgctcgc gccgggatcc
      901 ccattgcggg cgggcattcg atcgattcgg tcgaacccat ctacgggctg gccgcgctgg
      961 gcgtcgtgca tccccggcag gtcacgcgca ataccggcgc acacgccggc gacatcctga
     1021 tcctgggcaa ggcattggga gtgggcatat tatcggcggc gctaaaaaaa ggccgtcttg
     1081 acgacgcagg ctaccgcgcc atgatcgaca ccaccacacg cctgaatagc gtcggtcccg
     1141 acttggcggc actgcccggc gtgcatgcca tgacggacgt caccgggttt gggttgctgg
     1201 gtcacacact ggaactttgc cgcggcgctg gcctgacggc gcgcatccgc tatgccgatt
     1261 tgccctggat tcaaggggcg ttggaactgg ccgacgccgg catacatacg ggcgcctccg
     1321 cgcgaaattg ggcatcatac gaagacagcg tcgaattggc cgcccacttg cccgccgtcg
     1381 cccgcacact gttggccgat ccgcagactt cgggcggatt attggtgtcg tgcgcaccgt
     1441 ccgccgtgga tgccgtgctg gcgctattta gcgccggtga attcgaccag gccgcgatta
     1501 tcggcgagat ggtcgacgga gcggcgcgga ttattgcgga ataaaggcgg cgccataacc
     1561 ccttggcgcg cacggatttc gcccggcaaa atcgtactat gatgattgcc agatcccttt
     1621 ttcgaggaga atacattgga atcacgcatt gattacgccg ccgcttcgcc cgacgccgtg
     1681 aaagccatga tggcgctgga aggtgcgatg cgcagactcg gccttgaaca accattggtg
     1741 gaacttatca agctgcgggc atcgcaaatc aatggatgcg cattttgcgt cgatatgcac
     1801 accactgacg cgctgaaagc gggcgaaacc gaacggcggc tgaacagcgt tgtgacgtgg
     1861 cgcgaagcgc ccttcttcac agcgcgcgaa cgcgccgccc tgggatggac tgaggcgctt
     1921 acgcttattt ccgaaacgca tgctcccgat acagactacg aggcgttgcg cgctcagttc
     1981 acggaagccg agatggtcaa tgtgacgctt gccatcgcaa cgatcaattg ctggaa
//
LOCUS       DATCXU010000440         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_398696,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000440 DATCXU010000000
VERSION     DATCXU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398696"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIR04_09885"
     CDS             complement(<1..779)
                     /locus_tag="VIR04_09885"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461102.1"
                     /translation="MTQNINTQAAAIDVREVTLAYQTGADKVVAVDSVSFTIQAGKFF
                     VLVGPSGCGKSSLLMMMAGLQRPTQGQIICGDRPIERPDPDRTGVVFQEANLYPWLSA
                     QKNVEFPLMLKKVPAHERSQRAMEVLDLVGLANFSQRFPHELSGGMKQRVSIARGLVQ
                     RPAALLMDEPFAALDEQTRLTMGDELLRIWERTRQTLVFVTHSLTEAVYLADEIAVMS
                     AHPGRVMEIIPVPLPRPRTMEMMATEAFGQLRARIWQLIKKP"
     gene            complement(782..1540)
                     /locus_tag="VIR04_09890"
     CDS             complement(782..1540)
                     /locus_tag="VIR04_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9461103.1"
                     /translation="MKQHALKWLLHPLALLALLLVLWEAVAQSGLVNRMLLPPASKVA
                     IVLIDLLQRESVWQAIAITARETATSYLIAAPIGMAIGLFLAESRYWGAVFKPFFYFL
                     FSIPKSIFLPMFILAIGIGFTQKVAFGIFSTLFIVVISISTAVESVSANHKLVARCYG
                     ASRKQIAWHVYLPSMLPAILETLRLAMIFNFTGIMLAEMYVSREGMGHLIGRWGEDYM
                     LPQLLAGVVIVSCAAVLFNESVRVFEKKYGHWRT"
     gene            complement(1543..>2274)
                     /locus_tag="VIR04_09895"
     CDS             complement(1543..>2274)
                     /locus_tag="VIR04_09895"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461104.1"
                     /translation="ANAADMNMNGAYLVALGRNKGVKSKIVATGSTVYPGWYLVVPKN
                     SPIKSAADLAGKKVGVTANGSITDFLALWTARTNKVSFTRVPVGGGGLIPNLLNGNID
                     AAVVYSPLSFKLLNTGSAHSIIDFGKVMDPDLNAGWIATDDFITNHPKAVQGTLNAIF
                     GAVQYLQTHKDYAVKLIAELNDLPPEVAALEYQNSIMGASKDGAISPKAVEHSVELAT
                     QGGLTNLAPAADTFVTTFKPVPTQP"
BASE COUNT          468 a          657 c          669 g          480 t
ORIGIN      
        1 ggctttttaa tgagctgcca gatgcgtgcc cgcaactggc caaaggcttc ggtggccatc
       61 atttccatgg tgcgcgggcg cggcagcggt acggggatga tctccatcac gcgccctggg
      121 tgcgccgaca tgacggcaat ctcatcagcc agatacaccg cctcggtcag gctgtgcgtg
      181 acaaagacga gtgtttggcg cgtgcgctcc cagatgcgca gcagctcatc gcccatcgtc
      241 agacgtgtct gttcgtccag tgcggcgaag ggctcgtcca tcagtaaggc ggccgggcgc
      301 tggacaaggc cgcgcgcgat cgacacgcgt tgcttcatgc cgcccgagag ctcgtgtgga
      361 aaccgttgtg aaaagttggc cagccccacc agatccagga cctccatcgc ccgctggctg
      421 cgttcatgcg ccggaacctt cttgagcata agcggaaact cgacgttttt ttgcgcgctt
      481 agccaagggt acaggttggc ctcctgaaag acgacgccgg tccggtcggg atcagggcgc
      541 tcgatcggcc tgtcgccgca aatgatttgc ccctgcgtcg ggcgttgcag tccggccatc
      601 atcatgagga gcgaggactt cccgcagccg ctgggcccga ccagaacgaa aaacttgccc
      661 gcctggatcg taaaggagac gctatcgacc gcgacgactt tgtcggcccc ggtttggtac
      721 gccagcgtta cctcgcgcac atcgatggcg gccgcctggg tgttgatgtt ctgcgtcacg
      781 attacgttct ccagtgtccg tatttctttt caaacacccg gaccgattcg ttaaacagca
      841 ccgccgcgca cgagacgatg accacgcccg ccagaagctg cggcagcatg tagtcctcgc
      901 cccagcgccc gatcaggtgt cccatgcctt cgcgcgacac gtacatctcg gccagcatga
      961 tgccggtgaa gttaaatatc atggccaggc gtaacgtttc gagaatcgca ggcagcatgc
     1021 tcggaagata cacatgccag gcaatttgct tgcgcgaggc gccatagcag cgggccacca
     1081 gcttgtggtt ggcgctgacg ctttcaacgg cggtggaaat gctgatgacc acgatgaata
     1141 gggtggaaaa aatgccgaaa gcgacttttt gggtgaatcc gatgccgatc gccagaatga
     1201 acatcggcag aaagattgat ttgggtatgc tgaacagaaa gtaaaagaag ggcttgaaca
     1261 cagcccccca gtagcggctt tccgccagaa acaggccgat cgccatgccg atcggcgcgg
     1321 caatcaggta actggtggct gtctcgcgcg cggtgatcgc aatcgcctgc cagaccgatt
     1381 cgcgttgcaa gagatcgatc aggacgatgg ccacctttga agccggcggc agcagcatcc
     1441 gattgaccaa ccccgactgc gctaccgcct cccatagtac gagcaggagg gcgagcagcg
     1501 ccagtgggtg aagcagccac tttaatgcgt gttgcttcat cttcagggtt gcgtcgggac
     1561 cggtttgaat gttgtcacga acgtgtcggc ggcgggagca agattggtta aaccgccttg
     1621 cgttgccagt tcgacagagt gctcaacggc tttaggtgaa attgctccgt ctttggaagc
     1681 ccccatgatg gagttttggt attccagggc ggcgacttcc ggcggcagat cgttcagttc
     1741 ggcgataagc ttgactgcgt agtccttgtg cgtttgaaga tactgcaccg caccgaaaat
     1801 cgcattcaaa gtgccttgca cggctttcgg atggttcgtg atgaaatcat cggtggcaat
     1861 ccatcccgca ttcagatcgg ggtccattac tttgccgaag tcgatgatac tgtgggccga
     1921 accggtattt agcagcttga aactaagcgg actgtagaca accgccgcat cgatgttccc
     1981 gttcaaaaga ttgggaatca atccgccgcc gcccaccggc acccgcgtga aggacacttt
     2041 gttggtgcga gccgtccata gcgcgaggaa gtcggtaatc gatccattgg ctgttacccc
     2101 gaccttttta ccggccagat ccgcggcgct tttaattggc gaattttttg gcacgaccag
     2161 ataccatccc gggtacaccg tacttccggt ggctacgatc ttcgacttca cgcctttgtt
     2221 gcggcccagc gccaccaggt aagcgccatt catgttcata tcggctgcgt tggc
//
LOCUS       DATCXU010000441         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_754938,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000441 DATCXU010000000
VERSION     DATCXU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754938"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="VIR04_09900"
     CDS             <1..1924
                     /locus_tag="VIR04_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461105.1"
                     /translation="PAVLRLAAQFDRQRDRTLDPSSTPAQEQEPHQEDIPENRSPPST
                     WALLRLWRFARPYRVALLTGFALTLLSTAATLVAPYLTMPLMDNILIPFQNGTPIDYD
                     LVELYLGALLASSLLAWLLGWGRTYILAWVSERIGADLRTTTYQHLQSLSLQYFGGKR
                     TGDLIARIGSESDRICIFLSLHLLDFATDMLMIIMTAVILISINPWLAAATLIPLPFI
                     AWLIHMVRDRLRRGFERIDRIWSEITNVLADTIPGIRVVKAFAQEGREVARFREANNR
                     NLLVNDRVNTIWSLFSPTVTLLTEIGLLIVWGFGIWLVSYQQITVGVLTAFLTYISRF
                     YIRLDSMSRIVSFTQKAAAGAKRIFDILDHVSSVPEPAHPVRLDGVKGRIELRNVGFR
                     YGNRSVMRGINLDIAPGEMIGLVGHSGSGKSTLVNLICRFYDVSEGAIRLDGIDVRSL
                     SISDYRRNIGLVLQEPFLFFGTIAENIAYGRPEASRQEIIAAARAANAHEFILRLAHG
                     YDSLVGERGQALSGGERQRISIARALLIDPPILILDEATSSVDTTTEKEIQKALDNLV
                     RGRTTISIAHRLSTLRQADRLVVLDRGRIVEVGRHDQLLAQNGIYSQLYQAQQRGEEP
                     APSQSEWVEEGSGEIA"
     gene            1941..2408
                     /locus_tag="VIR04_09905"
     CDS             1941..2408
                     /locus_tag="VIR04_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HEY9461106.1"
                     /translation="MSRNDFRLSRDPFGRLVLKIGYRIYDSVVPVRSFPISAPNEGLA
                     LVGADGAELVWINSLDELSEDVRRLLEEELVSREFVPEIRRIRQVSSFATPSEWQVET
                     DRGDAVLVLKAEEDIRRLSATTLLIADTNGVQFLVRDLLALDKISRRLLDHFM"
     gene            complement(2388..3557)
                     /locus_tag="VIR04_09910"
     CDS             complement(2388..3557)
                     /locus_tag="VIR04_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461107.1"
                     /translation="MNKTGQSGAPGAGGIQFRGPANSLDNARESFLEELNSLGLGSCV
                     KVDRMADGDGEFVHFGLNELSAGDFGSDFDTMQLCGKLNLDTAGNPDDLAREILLAML
                     MGPVAFSFPTYDELVSAVRIRRNIVVAARKTALAFDTEEAERPAGDWNYSEARGFVLV
                     PGRPLIESLRKATQPERSGKLYSFSCYRATEYVMLLGLAQELERCNPGLSRRLQEQWE
                     TRAIMSGEFHDVFLHEYGTLSEPLPPRYYVPGDRVWFRNPDEHSSNVDGYEGSWVFYI
                     GNGLFTNFWKRDQPFTLVTKCVELYHWRNATYRNGDGKLQIDEDIVEDRVRQSLQDPL
                     EEERILKKMLRLREPAGVYLNGGCVDATREYVCCVRPGTSEIIFPHKNSLHKVVE"
BASE COUNT          749 a         1101 c          988 g          765 t
ORIGIN      
        1 cccggcggtt ctgcggctgg ccgcgcaatt cgatcgccag cgcgaccgga cgctggaccc
       61 gtcatcgacg ccggcgcaag agcaggagcc gcaccaggag gacatcccgg agaatcgctc
      121 gcccccctcg acctgggcac tgctgcgttt atggcgcttc gcccggccct accgagtcgc
      181 tctgctgacg ggcttcgcgc ttacactctt gtcgactgcc gcaaccctgg tggcgccgta
      241 cctgacgatg ccgctcatgg acaatatact gatccctttc cagaacggca cgcccatcga
      301 ttacgatctg gtcgagcttt atctgggcgc tttactggcg tcctccttgc ttgcctggtt
      361 gctgggctgg gggcgaacct acatcctggc ctgggtcagt gaacgcatag gggcggacct
      421 gcgcacaacc acctatcagc atctgcaaag cctttccttg caatattttg gcggcaaacg
      481 caccggcgac ctgattgccc gtatcggttc ggaatccgac cggatctgca tattcctgtc
      541 gctgcacttg ctggatttcg cgaccgacat gctgatgatc atcatgacgg ccgtgatcct
      601 gatttccatc aacccctggc tggcagccgc caccctgatt cctttgccgt ttatcgcctg
      661 gctgatccat atggtgcgcg accggctgcg ccgcgggttc gagagaattg atcgcatctg
      721 gtccgagatc accaacgtgc tggccgacac cattccggga attcgcgtcg tcaaagcctt
      781 tgctcaggaa ggccgcgaag tcgcgcgctt tcgcgaggcg aacaatcgca atttgctggt
      841 gaacgaccgg gtcaacacca tctggtcgct gttttcgccg accgtcactt tattgaccga
      901 aatcggcttg ctgatcgtat ggggtttcgg catttggctg gtctcgtatc agcagatcac
      961 cgtgggcgtt ttgaccgcct tcctgacgta tatcagccgg ttttacatcc ggctcgactc
     1021 catgagccgc atcgtttcgt tcactcaaaa agccgccgct ggcgcgaagc gcatcttcga
     1081 tattctggac cacgtttcca gtgttcccga acccgctcat ccggtccgcc tggacggcgt
     1141 gaaagggcgc attgaacttc gcaacgttgg gttccgttat ggcaacagat cggtaatgcg
     1201 cggcatcaac ctcgacatcg cgccaggaga gatgatcggc ctggtgggtc atagcgggtc
     1261 gggcaaaagc acgcttgtaa acttgatatg ccgcttctac gacgtatccg aaggggcgat
     1321 acggcttgac ggaatcgacg tcaggtcgct tagcatttcg gactatcggc gcaatatcgg
     1381 cctggtgctg caagaaccat ttttgttctt cgggaccatt gctgaaaaca tcgcttatgg
     1441 caggccggaa gccagccggc aagagatcat cgccgcggcg cgcgccgcga acgcgcacga
     1501 attcatattg cgcctggcgc acggctacga ttccctggtt ggcgagcgcg ggcaagcctt
     1561 gtcgggcggc gagcgtcagc gaatttcaat cgcccgcgcg ctgctcattg acccgcctat
     1621 tctaattctg gacgaagcca cctcgtcggt cgacaccacc accgaaaaag aaattcagaa
     1681 ggcgctggac aatctggttc gcggacgcac gacaatttcg attgcgcacc gcttgagcac
     1741 cctgcgccaa gccgaccggc tggtcgtcct ggaccgggga agaatcgtcg aagttggccg
     1801 gcacgaccag ttgcttgcgc aaaacggcat ttattcccaa ctgtaccagg cccagcaacg
     1861 tggagaagaa cccgctccca gccaaagcga atgggtcgag gaaggcagcg gagaaatagc
     1921 atgaatcagg caacgaaccc atgagccgca atgattttcg tctgagccgc gaccctttcg
     1981 gccgcctggt cctcaaaatc ggctaccgca tctacgacag cgtcgtgccc gtgcgcagct
     2041 ttcccatatc cgcccccaac gagggtctgg cgctggttgg cgccgacggc gctgagctgg
     2101 tctggataaa cagcctggac gaattatccg aagacgtgcg ccggcttctg gaagaagaac
     2161 tggtcagccg cgaatttgtg cctgaaatcc ggcgcatccg ccaggtttcc agctttgcca
     2221 cacccagcga atggcaagtc gaaaccgacc gcggcgatgc cgtgctggtg ctcaaagcgg
     2281 aagaggacat tcgccggctg tcagccacaa ctttgttgat tgccgatacg aacggagttc
     2341 aatttctggt ccgcgactta ttggcgctgg acaagatcag ccgccggcta ctcgaccact
     2401 ttatgtaagg aatttttgtg cggaaaaata atttccgacg tgcccggccg gacacagcaa
     2461 acgtattccc gcgttgcatc cacgcagccg ccgttcaggt agacgccagc aggctcgcgc
     2521 aaacgcagca ttttctttag aatccgttcc tcttcaagcg ggtcctgcaa agattgccgc
     2581 actctgtctt cgacaatatc ttcgtcgatc tgcagtttgc cgtcgccatt tcgataggtt
     2641 gcattgcgcc aatgatagag ctccacgcat ttcgtaacca gggtaaaggg ttgatcccgt
     2701 ttccagaaat tcgtaaataa gccgttgccg atataaaaca cccacgagcc ttcataacca
     2761 tcgacattgg acgagtgctc atcggggttg cgaaaccaga cccggtcgcc cggcacgtag
     2821 tagcgcggcg gcagtggctc gctcaaggtt ccgtattcgt gcaaaaacac gtcgtggaac
     2881 tcgcccgaca taatggcccg cgtttcccac tgttcttgca ggcggcgcga aagcccggga
     2941 ttgcaccgct ccagttcctg cgcaagcccc agcaacatga catattccgt tgcccggtag
     3001 caggaaaatg aataaagttt gccagagcgc tcgggctgcg tggccttgcg caaagattca
     3061 atcagcggtc ttccgggcac caggacaaaa ccgcgcgctt cagaatagtt ccagtcgcca
     3121 gcagggcgct cggcctcttc cgtatcgaaa gccagcgctg tcttgcgagc cgccacaacg
     3181 atgttccttc gaatacgcac tgccgacact aattcgtcat acgtggggaa agaaaaagcg
     3241 accggaccca tcagcatggc cagcaggatc tcgcgcgcca ggtcatcggg gttgccggca
     3301 gtatccaggt tcagtttccc gcacaactgc atcgtgtcaa aatcggagcc gaaatcgccg
     3361 gccgataatt cgttcaagcc aaaatggaca aactcgccat cgccatctgc catgcgatcg
     3421 accttgacgc aactgccaag acccagtgag ttcaattctt cgagaaagct ttctcgcgcg
     3481 ttatcgagac tgttcgcggg accccgaaac tggatgcctc ccgctccagg cgcaccggac
     3541 tggcctgtct tgttcatttg cgaattcctc tccatggaag ctcaacgctt cgctgcggtt
     3601 tcg
//
LOCUS       DATCXU010000442         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_524892,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000442 DATCXU010000000
VERSION     DATCXU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524892"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2714
                     /locus_tag="VIR04_09915"
     CDS             <1..2714
                     /locus_tag="VIR04_09915"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY9461108.1"
                     /translation="AHVSKPFVIQFPPPNVTGTLHMGHAFNQTVMDGLARYHRMRGDD
                     TVLIPGTDHAGIATQIVVERQLDAQHSSRHELGRERFVERVWEWKRKSGNAITEQFRR
                     LGSSADWSREYFTMDDNLSRGVVEAFVRLHEQGLIYRGKRLVNWDPVLGTAVSDLEVI
                     SEEEDGFMWEIRYPLCEPAGDMTGLVVATTRPETMLGDVAIMVHPEDTRYAHLVGRKV
                     KLPLSEREIPIIADDYVDREFGTGVVKVTPAHDFNDYAVGQRHGLKLINVLTLDAKIN
                     ENGPAAYQGLDRFDARKKVVEDLNALGLLESVKPHKLMVPRGDRTRTVIEPMLTDQWF
                     VAMSKPAPAGTRHPGKSITDVALEVVADGRITFFPENWTTTYNQWLGKIQDWCISRQL
                     WWGHQIPAWYAEDGQIFVARNEAEALKKAAAAGINGPLKRDEDVLDTWFSSALVPFTD
                     LGWPEQTADLKRYLPSSVLVTGFDIIFFWVARMVMMSMHMTGEVPFQTVYVHGLVCDM
                     EGKKMSKSKGNTIDPVDLIDGIDLERLLVKRTTGLMNPRQAASIKKKTAKDYPNGFPA
                     YGADALRFTMAAYATLGRNINFDLKRCEGYRNFCNKLWNATRFVLMNTEGHRLSDDDR
                     GELSFADRWIVSELQSLSREIEQGFADYRFDNIANALYRYIWDEYCDWYLELAKVQIQ
                     NGTPAQQCGTRHTLIQVLEAVLRLAHPIIPFITEELWQKVALPAGKRHASEQTSIGIQ
                     PYPCPNPALTDAIATAQVVELKAQIEAVRALRGEMNLSPAQRIPLVAQGDSDTLQRNG
                     PYLAAMAKLSGIEIVPKLPGIDAPVQIVGATELMLRVEIDVDAERTRLGKEISRLESE
                     INKANNKLSNASFVQRAPAAVVAQEKARVAQFTETLNKVNEQFSKLA"
BASE COUNT          664 a          769 c          764 g          549 t
ORIGIN      
        1 ccgcacatgt cagcaagccg tttgtcatcc agtttccgcc acccaacgtc accggcacgc
       61 tgcatatggg gcatgcattc aatcaaacag tcatggatgg tctggctcgc tatcaccgca
      121 tgcgcggcga cgatacggtg ctcattcctg gcaccgacca tgcgggaatc gcaacacaga
      181 tcgtggttga gcgtcaactg gatgcccaac atagttcccg ccacgaactc gggcgcgagc
      241 ggttcgttga aagagtctgg gaatggaagc gaaaatcggg aaacgccatc accgaacaat
      301 tccggcgcct gggctcctcg gccgactgga gtcgcgaata cttcacgatg gacgacaatt
      361 tgtcgcgcgg cgtggtcgaa gcctttgtgc gcctgcacga acaaggcctt atttaccgcg
      421 gcaaaaggct ggtcaactgg gaccccgttc tgggtacggc cgtttccgac ctggaagtca
      481 tcagcgagga agaagacggt ttcatgtggg aaatccgcta ccccctgtgc gagccggcgg
      541 gagatatgac cggccttgtc gttgccacca ctcgtcctga aaccatgctg ggcgacgtgg
      601 ccatcatggt tcatcctgag gacacgcgct atgcccacct ggtaggccgc aaagtcaaat
      661 tacccttaag cgagcgcgag atcccgatta tcgccgacga ctacgtagat cgtgaattcg
      721 gcacgggggt ggtcaaagta acgcccgcgc acgacttcaa cgactatgcg gtcggccagc
      781 gccacggcct gaaattgatc aatgtattaa cgcttgacgc caaaataaac gaaaatggcc
      841 ccgccgcgta ccaggggctg gaccgtttcg acgcccgcaa aaaagtggtt gaggacctga
      901 acgcgcttgg tctgctcgaa agcgtaaagc cgcacaagct catggttccg cgtggcgacc
      961 gcacccgtac cgtaatcgaa ccgatgctta ccgaccagtg gttcgtggcc atgagcaagc
     1021 ccgccccggc cggcacgcgt catccgggga aaagcatcac cgacgtggct ctggaggttg
     1081 ttgccgatgg ccggatcacg ttcttccctg aaaactggac caccacctat aaccagtggc
     1141 tggggaaaat tcaggattgg tgtatttcgc gacagctttg gtggggtcat cagattcccg
     1201 cctggtacgc cgaagatggt cagattttcg ttgcacgcaa tgaagctgaa gcgttgaaaa
     1261 aggcggccgc ggccgggatc aatggtccgc taaaacgcga cgaggatgtg ctggatacct
     1321 ggttctcatc ggctctggtg cctttcaccg atctgggctg gcccgaacaa accgccgacc
     1381 tcaaacgtta tcttccgtcc agcgtattgg taaccggctt cgacatcatt ttcttctggg
     1441 tcgcccgcat ggtcatgatg agtatgcaca tgacgggcga agtgcctttc caaaccgtct
     1501 acgtgcatgg ccttgtgtgc gatatggaag gcaagaagat gagtaaatcc aagggcaata
     1561 caatcgaccc ggtcgacctg atcgacggaa tcgatctgga acgtctgctc gtcaaacgca
     1621 caacagggtt gatgaaccca aggcaagcgg ccagcatcaa gaagaaaacc gcaaaagatt
     1681 atcccaatgg ctttccggcc tacggcgcgg atgcattgcg ttttaccatg gccgcctacg
     1741 cgacgctggg gcgcaatatc aatttcgatc ttaagcgttg cgaaggctac cgcaattttt
     1801 gcaacaaact ttggaacgcc acccgctttg tgctcatgaa caccgaaggc catcggcttt
     1861 cggatgacga ccggggcgaa ctgtcgtttg ccgaccgctg gatcgtgagc gaacttcaaa
     1921 gcttgtcacg cgaaattgag cagggttttg ccgactaccg tttcgacaat attgcgaatg
     1981 ccttgtatcg ctatatttgg gatgagtatt gcgattggta tctggagctg gcaaaagtgc
     2041 aaattcaaaa cggcacgcca gcgcagcaat gcggaacgcg gcatacactc attcaagttc
     2101 tggaagccgt gctgcgcctg gcgcacccga ttattccgtt cattaccgaa gagctatggc
     2161 aaaaagtcgc gctgccggcc ggcaagcgcc acgccagcga acaaacaagt attggcatac
     2221 agccctatcc ctgccccaac ccggctttaa ccgacgcgat agccactgca caagttgtgg
     2281 aacttaaagc gcaaatcgaa gccgtgcgtg ccttgcgcgg ggaaatgaat ttatccccgg
     2341 cacagcgcat acccctcgtt gctcaaggtg acagcgatac cctgcagcgc aacggtccgt
     2401 acctggcggc aatggcaaaa ctaagcggta ttgaaatcgt gccgaagctg ccagggattg
     2461 atgcgcccgt acaaattgtt ggcgccactg aactgatgct gcgtgttgaa attgacgtcg
     2521 acgccgaacg cacgcgtttg ggcaaagaaa tcagccggct ggaaagcgaa atcaacaagg
     2581 caaacaataa attatcgaac gccagcttcg ttcagcgggc gcccgcggcc gtcgttgcgc
     2641 aagagaaagc gcgcgtggcg cagtttactg aaacgctaaa taaagttaac gagcagtttt
     2701 caaaactggc gtagagcaat cgctggcaag ctgcgccaaa aaacgc
//
LOCUS       DATCXU010000443         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_45502,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000443 DATCXU010000000
VERSION     DATCXU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45502"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VIR04_09920"
     CDS             <1..619
                     /locus_tag="VIR04_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEY9461109.1"
                     /translation="APTSNRHDATTNYEVDKTITHVKEPLGQLRRLSVAVVINDQHDK
                     DGNIKPMDQAGMAKLNGLVQQAMGYSATRGDTLTIISSPFNDTSVEVLPIWKDPVYQT
                     MAMQLGKYLLIALITLLLWRNLLKPILKNIVNPENMVAHPRSEAAGAAAGAAAERANA
                     EAAKKRASEISRYEDNLSVARTMAEKDPRAVAMVLRSWMDKDGKQ"
     gene            606..1607
                     /gene="fliG"
                     /locus_tag="VIR04_09925"
     CDS             606..1607
                     /gene="fliG"
                     /locus_tag="VIR04_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY9461110.1"
                     /translation="MANNDVNLDRCAVLMMSLGEDAAAEVFKYLSAREVQKVGLSMAN
                     LKQITRHDVDSVLEEFRHEADQFMAVTVGSDDYIRSVLTKALGNDRAAGLIEDILDAG
                     DGANGIDALNWLDANSVAELISDEHPQIIATILVHLERDRASAVLAILNERLRNDVIL
                     RIATFGGVQPAALHELTDVLNSMLSGQGAKRSKMGGVRTAAEILNFMNSAEEDAVVAS
                     LRALDADLAQRIVDEMFVFENLLDIEDHAIQLILKEIDTTVLTIALKGASEELRDKFL
                     KNMSTRAAEMLRDDLDAQGPVRMSKVEEEQKAILQVARRLAEAGEFSIGGTGKDEYV"
     gene            1600..2364
                     /gene="fliH"
                     /locus_tag="VIR04_09930"
     CDS             1600..2364
                     /gene="fliH"
                     /locus_tag="VIR04_09930"
                     /inference="COORDINATES: protein motif:HMM:NF004270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEY9461111.1"
                     /translation="MSDSAKNRLHSRVENSWERWEMTAFDGSSVREKKMPPLDATAAG
                     AVQTELDLEQLRSATSARARAQGYAAGHAEGINAGTAEGRKAGEIKGYETGFSAGHAE
                     GRAQAQQAAQQLQSLADSFASAIGTIEAEMGQALIALAINIAQQVLRSTLAEHPDKIL
                     DLVRDIVLADLDKDAVLQLRVNPADLTLVEEYLAADSGIKNWRINGDETIERGGCTAE
                     TELGNIDATLQTRWRRVISSLGRASVSDGGRDGAGH"
     gene            2348..>2844
                     /locus_tag="VIR04_09935"
     CDS             2348..>2844
                     /locus_tag="VIR04_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEY9461112.1"
                     /translation="MAPAIKRLSSATARWQAQLTAAGARVNATEAMGISGRITRATGL
                     VLEATGLRLAVGAACKIEITPGQNHWADAEVVGFHGHTLYLMPQSDISGLPPGARVVS
                     VEPSAQNIIELPLYETGEIGAPVLTRHLPVGNSLLGRVLDGAGRPLDDLGPLKHVTPA
                     PLGGKT"
BASE COUNT          663 a          812 c          851 g          518 t
ORIGIN      
        1 cgctcccacc tcgaaccgcc acgacgcgac gactaactat gaggtcgaca aaacgatcac
       61 ccacgttaaa gagcctctgg gtcagttgcg gcgtctatcg gtggcggtgg ttatcaacga
      121 ccagcatgac aaagatggca acatcaaacc aatggaccag gccggcatgg ccaaacttaa
      181 cggccttgtc cagcaagcca tggggtattc agcaacgcgt ggcgatacgc taaccataat
      241 cagcagcccg ttcaacgaca cttccgtcga ggttctgccc atttggaaag accctgttta
      301 ccagacaatg gccatgcaac tcgggaaata cctgctgatt gcgctgatca ctcttttact
      361 ttggcgcaac cttctgaaac ccatactaaa aaatatcgtc aatcctgaaa atatggttgc
      421 gcatccgcgc tctgaagcgg ccggggccgc cgccggagcc gcggcggagc gcgctaatgc
      481 ggaggcggca aaaaagcggg cttcagagat ttcccgctac gaagacaatt tgagtgtcgc
      541 acgcacgatg gcagagaaag atccgcgcgc cgtcgccatg gtgttgcgct catggatgga
      601 caaagatggc aaacaatgat gtaaacctgg atcgttgcgc agtgctgatg atgtcgctgg
      661 gagaagacgc ggcagccgag gtgtttaagt acctgtcggc gcgagaggtg caaaaggtcg
      721 ggttatccat ggccaacctt aagcagatta cccgccatga tgtcgacagc gttctggagg
      781 aattccgcca cgaagccgac cagttcatgg cggtaactgt cggctcggac gactatatcc
      841 gcagcgttct gaccaaagcg ctgggcaatg atcgcgctgc cggactgatt gaagacattc
      901 tggatgccgg tgacggcgcc aacggcatcg atgcgcttaa ctggctcgac gccaatagcg
      961 ttgccgaact gatcagcgac gaacacccgc aaattatcgc caccattttg gtgcacctgg
     1021 agcgcgaccg cgcatcggcc gttctggcca ttctcaacga gcggctgcgc aatgacgtca
     1081 tactgcgaat cgcgacgttt ggcggcgtgc agccagcggc actgcatgaa ttaaccgacg
     1141 ttttaaatag catgctttcc ggccaggggg ccaaacgcag caaaatgggt ggtgtgcgca
     1201 ccgccgccga aatacttaat tttatgaatt cggccgagga agacgccgtg gtcgccagcc
     1261 tgcgcgcgct ggatgcggac ttggcgcagc gcatcgtcga cgagatgttt gtctttgaaa
     1321 acctgctcga catcgaagac cacgccatcc aactgattct taaagaaatc gacaccaccg
     1381 tgctgaccat cgcgcttaag ggggccagcg aagagttgcg cgataaattc cttaaaaata
     1441 tgtcgactcg cgccgctgag atgttgcgcg atgacctgga cgctcaaggt ccggtgcgca
     1501 tgtctaaagt cgaagaagaa cagaaggcga ttttgcaagt ggcgcgccgg ctggctgaag
     1561 cgggtgaatt cagcatcggc ggaacaggaa aggacgaata tgtctgatag cgcgaaaaac
     1621 cgcttgcatt cccgcgtgga gaattcttgg gagcgctggg aaatgaccgc gttcgatggg
     1681 tcttcggtac gcgagaaaaa aatgccgccg ctagatgcaa cagcagcggg cgccgttcaa
     1741 accgaactcg atctcgaaca actgcgaagc gcgacaagcg cgcgggcccg cgcgcaaggc
     1801 tatgccgcag ggcacgcgga gggcatcaac gccggaacgg ccgaaggccg caaagccggc
     1861 gagataaagg gctacgaaac cggttttagc gccggccacg ccgaaggtcg cgcacaggcg
     1921 caacaggccg cgcaacaatt gcaaagtctc gcggattcgt tcgccagcgc gatcggcacg
     1981 atcgaggcag aaatgggcca ggcgcttatt gcgctggcga tcaacattgc ccagcaagtt
     2041 ttgcgaagca cgcttgccga gcaccccgat aaaatcctgg atctcgtgcg cgatatcgtt
     2101 cttgccgatc tcgacaaaga tgcggtattg cagttgcgcg tcaatccggc cgacttgacg
     2161 ctggtcgaag aatacctggc ggccgattcc ggcataaaaa actggcgcat caatggcgac
     2221 gagacaattg aacggggcgg ctgcacggct gaaaccgagt tgggaaacat tgacgccacg
     2281 ctgcagacgc gctggcgccg agtcatttct tctctgggca gggcgtccgt ttcagacggg
     2341 ggccgcgatg gcgccggcca ttaaacgcct gagctcggcc acggcgcgat ggcaagccca
     2401 attaaccgcc gccggggccc gggtcaatgc gactgaagcc atgggcatta gcgggcggat
     2461 cacgcgagct acaggccttg tgcttgaggc gaccggtttg cggcttgccg tgggcgccgc
     2521 gtgcaagatc gaaatcacgc ccggacaaaa ccattgggcc gatgcggagg tcgtcggttt
     2581 tcatggccac acactctatc tgatgccgca aagcgacatc tcgggtttgc cgcccggggc
     2641 acgggtcgtt tcggtcgaac catccgcaca aaacatcatc gaactcccgc tatacgaaac
     2701 cggcgaaatc ggcgcgccag tactgacgcg ccatttgccg gtgggcaata gtctgctggg
     2761 ccgcgtgctg gatggcgcgg gccgcccgct tgacgacctt gggcctctca aacatgtcac
     2821 tccggcgccc cttggcggca aaac
//
LOCUS       DATCXU010000444         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_685159,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000444 DATCXU010000000
VERSION     DATCXU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685159"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VIR04_09940"
     CDS             complement(<1..1595)
                     /locus_tag="VIR04_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY9461113.1"
                     /translation="MLSLSYELNFAFALAPVALMSLAALWAWCSHVSVSRMWRQFNAL
                     ALTALAFSLIGLVSQLAFAPTHSTVAAGADSWLAVNQFGAWVSLLVQLLGAAIGIYSS
                     NHLKGEPRQPVYIAALAVVLTAVHLLLLANHWNVLIGAWAAIGYAMHHLLCFYRDRPF
                     ALLAAHKKMVTDRAADLLLIVAAILAWSQVGSGSLSALWAHIEAGHTSPALNWSGICL
                     VLAVIARTALMPVHGWLVQVMEAPTPVSALLHAGVVNLGGYVLVLFAPLLGVSLAARW
                     LLVVFGLVSAVVAGLVLLTRVSQKVHLAWSTIAQMGFMLLECGLGLYLFAALHLLGHS
                     LYKAHNFLNASSAVVECNRKLITGTVRNSGLSMIIAAPAALIIVFVLQSVIGNRAWPW
                     WWSAVLALAWAPLLWVPRSKESGSGLVFVRLMTALAATAFLTLVAGIMDQAPIGAGSD
                     PLPGAGVVVLAGMGALYLGMTVFQLWPQSVITVRRWIYAGFYMDEGYTYWVLRLWPVR
                     WAGAGAGGETMQAWRLQRPARERT"
     gene            1690..2014
                     /locus_tag="VIR04_09945"
                     /pseudo
     CDS             1690..2014
                     /locus_tag="VIR04_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015695.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          505 a          642 c          535 g          332 t
ORIGIN      
        1 gttcgctcgc gggcaggtcg ctggaggcgc caggcttgca tggtttcgcc gccggccccc
       61 gccccggccc agcggacagg ccacagcctc aacacccagt atgtgtagcc ctcatccata
      121 taaaagccgg cgtatatcca gcggcggact gtaataaccg attgtggcca gagctggaat
      181 accgtcatcc caaggtacaa cgcacccatc ccggccagga caacgacacc cgcgccgggc
      241 agcggatcac tgcctgcacc gatcggagct tgatccatga tgccagcaac gagcgtgagg
      301 aaagcagttg ccgccaacgc cgtcatcaac cgcacgaaca ctaacccgga cccgctttct
      361 ttcgaccttg gcacccacag gagcggcgcc catgcgagcg caagcacagc gctccaccac
      421 cacggccatg cgcgattgcc gatcacagac tgaagaacga atacgatgat aagcgcggcg
      481 ggggccgcaa tgatcatgct caagccagaa ttccggactg tacccgtaat aagcttgcga
      541 ttacactcca cgaccgcgct ggaggcgttt aagaagttgt gcgccttgta aagcgaatgg
      601 cccagcaggt gcaacgccgc gaaaagatac aaacccaaac cgcattcgag aagcatgaaa
      661 cccatctggg cgatcgtaga ccacgccagg tgcactttct ggctgacgcg cgtcaggagc
      721 accaaccccg ccacaaccgc ggaaaccagt ccaaatacca ccagtagcca gcgcgcagcg
      781 agcgatacac caagcaaggg cgcaaacaac accagcacgt agcctccaag attgaccact
      841 ccggcatgca acaatgccga cactggcgtc ggggcttcca tgacctgcac cagccagcca
      901 tgcaccggca tcagtgccgt gcgcgcgatg accgccagca ccaggcaaat gccgctccaa
      961 ttgagcgccg gggaagtatg gccggcctcg atatgcgccc acaaggcgga caacgacccg
     1021 ctgcccacct gcgaccatgc aagaatcgct gcgacaatca ggagtaagtc ggccgcgcga
     1081 tccgtcacca ttttcttgtg cgccgccagc aatgcaaacg gccggtccct gtagaagcac
     1141 aacaggtggt gcattgcgta cccaatggcc gcccacgcgc caataagaac gttccagtgg
     1201 ttggccagca gcagaagatg cacggcagtc agtacaacag ccaaggcggc aatgtatacc
     1261 ggctggcgcg gctcgccctt cagatgattg gacgaataaa tgccaatggc cgcaccgagc
     1321 aactgcacca ggaggctcac ccaggcgccg aactggttca cggcaagcca gctatcggca
     1381 cccgccgcaa ccgtgctgtg cgttggtgca aatgccaact gcgagacgag gccaatcaga
     1441 gaaaaggcca gtgccgtgag cgcgagcgca ttgaactgac gccacatgcg cgatacggat
     1501 acgtggctgc accacgccca caacgccgct agcgacatca gtgcgacggg cgccagcgca
     1561 aacgcgaaat tcaactcata cgacagactc aacatgcacc ctgcgaaaca taacccgtgc
     1621 attcttcatc ttttcatgta ttctgtaaat tacatttata atatccttcc gttctctaaa
     1681 atagaactta tggatataag tcaactgaac ttccggcatt tgttttactt ctggcgtgta
     1741 gccaagatcg gatctctcac gcacgcagcc aggcaaattc atacttcgca gtccgccctt
     1801 tcgacgcaaa tcaagcaatt ggagagccgc ctgggcaagc ctttgttcga tcgggaccgg
     1861 cggcacatga actcaccgct acgggccagc agatatttgc ctacgcggac agtatcttcg
     1921 ggctgggtga acaaatgttg ggatggctgg aaggtcgcga cgaaggcaca acccacgtcc
     1981 gtgtgggaag cgtttcgacc atgtcacgca actt
//
LOCUS       DATCXU010000445         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_988482,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000445 DATCXU010000000
VERSION     DATCXU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988482"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VIR04_09950"
     CDS             <1..769
                     /locus_tag="VIR04_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY9461114.1"
                     /translation="TALAAGCAHVSNPNPDDPWESYNRSMYTFNDNFDRALVKPVAQG
                     YVALTPKPVRTCIHNIFNNVYDVWSAFNSFLQGRIPDGINTVGRVLFNSTMGIGGCID
                     VASMNGSRRISTDFGVTLGVWGFGPGPYLVLPFAGSSTVRDGTALVGSLASGVSLYTP
                     LAAIDNFPLRNSIMALGFIDLRASLLDTDKLVNEVALDRYSFIRDAYLQRRQAQIRNR
                     STEAPLPDYSTDTLPDYSTGASPAQPSAAAPDAKTKR"
     gene            819..1433
                     /locus_tag="VIR04_09955"
     CDS             819..1433
                     /locus_tag="VIR04_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461115.1"
                     /translation="MASRWLAAGLLCLGLTGMASAEDAVNPTAAPDQFVQAVGNNTLN
                     AIKQDKTLKTGDLAKINRIVNQYVLPYVNLQKTTRLAAGRYWQQATPAQQQSLVNAFK
                     GTLIRTYSGALTNVDPQSKLTILPFRGEANADDVVVRSTLSQGNGPTIGLDYRLEKTP
                     KGWKIYDLNVEGIWLIQNYRNQFAQQISQNGIDGLIQALNQQNK"
     gene            1554..2345
                     /locus_tag="VIR04_09960"
     CDS             1554..2345
                     /locus_tag="VIR04_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461116.1"
                     /translation="MPAVTLDHISKIYPVNVRGWKKWLSRAPVEKGFQALDNISLHIE
                     HGEFFGLLGPNGAGKTTLISILAGLARATSGKASICGHDVVTQFKQSRRSLGVVPQEI
                     VYDPFFTVRETLRIQSGYFGLHHNDDWIDEILSNLGLTDKAEINMRALSGGMKRRVLV
                     AQALVHRPPVIILDEPTAGVDVDLRRTLWGFISRLNKEGHTVMLTTHYLEEAEALCGR
                     IAMLKGGHLVALDTTQALLARVGGADLEDAFVRIMHGDNMLEVLA"
     gene            2375..3133
                     /locus_tag="VIR04_09965"
     CDS             2375..3133
                     /locus_tag="VIR04_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461117.1"
                     /translation="MGSGFSTLLHKELSRFWKVGLQTIAAPVVTALLYLLIFAHVLEG
                     RVQVYGTVPYTSFLIPGLIMMAMLQNAFANPSSSLIQSRITGNLVFILLPPFSHREIF
                     GAYMLASIARGVCVGVCVWLVALFFVALPAANPVWIVVFAILSCGIMATLGIIAGLWS
                     EKFDQLAAFQNFLIVPATFLSGVFYSIHSLPPFWQAVSRWNPIFYTIDGFRYGFFSAS
                     DVSPWQSLAVVTVVFIVLSGFTLRLLASGYKLRS"
     gene            3139..3387
                     /locus_tag="VIR04_09970"
     CDS             3139..3387
                     /locus_tag="VIR04_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY9461118.1"
                     /translation="MQPTPEQVRQYIADHLSCEFIDVRGDGAHFEALIVSSAFEGKRL
                     VARHQLVYAALGERMKAEVHALSMRTLTPAEHQASLNG"
BASE COUNT          695 a         1054 c          907 g          752 t
ORIGIN      
        1 gacggcgctt gccgccggat gcgcccatgt cagcaatccc aatcccgacg atccgtggga
       61 aagctataac cgcagcatgt acacgtttaa cgataatttc gaccgtgccc tggtcaaacc
      121 tgtcgcacaa ggttatgtgg cgcttacacc caaacccgtg cgaacctgca tacacaacat
      181 tttcaataac gtgtacgacg tctggtcggc attcaacagc tttcttcagg gacgcattcc
      241 agacggcatc aacacggtag gccgggtcct gttcaattcc accatgggca tcggcggttg
      301 tatcgacgtc gcttccatga atggttccag acgcatcagc actgatttcg gcgtgacact
      361 gggcgtctgg ggattcgggc ccggacccta tctggtgctg cctttcgcgg gttccagcac
      421 cgtgcgcgac ggcactgcgc tggtcgggtc gctggccagc ggagtgtctt tatacacgcc
      481 tcttgccgcg atcgacaact tcccgctgcg caattcaatc atggcgctgg gattcattga
      541 tctgcgcgcc agcctgctcg acacggacaa gctggtcaac gaagtggcgc tcgatcgtta
      601 cagcttcatc cgcgacgcct atttgcaacg ccgccaggct caaatacgca accgctcgac
      661 ggaagcgccg ctgcccgatt actcgacgga cactcttcct gactattcga ctggcgcctc
      721 gccggcccag ccttctgccg cggcgcccga cgccaagaca aaacggtaaa tggagcccca
      781 tgatgactac ttttctttcg accgccccaa cctgcaagat ggcatcgcgc tggcttgccg
      841 caggcctgct ctgcctggga ttgaccggca tggcgagtgc cgaagatgct gtcaacccga
      901 ctgccgcccc tgaccagttc gtgcaggccg tgggcaacaa cacgctgaac gcgatcaagc
      961 aggacaaaac ccttaaaact ggcgatctgg ccaaaatcaa ccggatcgtg aaccagtatg
     1021 tcctgcccta cgtcaacctt cagaaaacca cccggctggc ggctggccgc tactggcagc
     1081 aggccacgcc ggcgcaacag cagagcctgg tcaatgcgtt caaaggcacg cttatccgaa
     1141 cgtatagcgg cgcactgacc aacgtcgatc cccagtcgaa attgacaatc ctgccgtttc
     1201 gcggcgaggc caacgccgac gacgtggtcg tgcgatccac gctatcgcag ggcaacggcc
     1261 cgacgatcgg ccttgattac cgtcttgaaa aaacgccaaa gggatggaaa atttacgatc
     1321 ttaacgttga aggtatttgg ctgattcaaa actatcgcaa ccagtttgcc cagcaaatct
     1381 cgcaaaatgg catagatggc ctgatccagg cgctgaacca gcaaaacaaa taaactgtgc
     1441 gcacggccgg ccgctccgtc ctgaccggtg cagcggctgc aaaagcggct taatagtttt
     1501 cacttatata atagatggct tggcccaata gtgggccagg cctttttatt atcatgccag
     1561 ccgtcactct cgatcacatc tcaaagatct accccgtcaa cgtgcgagga tggaagaaat
     1621 ggttgtcccg ggctcccgtc gaaaagggct ttcaggcgct ggacaacatc agtttacata
     1681 tcgagcacgg cgaatttttt ggcttgctcg gtcccaacgg cgccgggaaa acaacgctca
     1741 tctcaatcct ggccggcctg gcgcgcgcca catcgggcaa ggccagcatt tgcggccacg
     1801 atgtggttac ccaattcaaa caatcgcgcc gctcgctggg cgtcgtcccg caggagatcg
     1861 tctacgatcc tttttttaca gtgcgcgaaa ccttaaggat tcagtcgggt tatttcggcc
     1921 tgcatcacaa cgacgactgg atcgacgaaa tcctgagcaa cctgggcttg accgataaag
     1981 ccgaaatcaa tatgcgcgcc ttgtccggcg gcatgaagcg gcgcgtgctc gtagcgcaag
     2041 cgctggtgca ccgcccgccc gtcattattc ttgacgagcc caccgcaggc gttgacgttg
     2101 acttgcggcg cacgttatgg ggctttattt cccggctcaa taaagagggg cacaccgtca
     2161 tgctgaccac tcattatctg gaagaggccg aggcgctctg cggccgcatc gccatgttga
     2221 aaggcggcca tcttgtggcg cttgacacga cccaggcgtt gctcgcccgg gtcggcggcg
     2281 ccgatctcga ggacgcgttc gtgcgcatca tgcatggcga caacatgctc gaggtcctgg
     2341 cttgagcgcg ccgattattc aaccgcatcc cggcatgggc tcgggatttt caacgcttct
     2401 gcataaagaa ttatcgcgat tctggaaagt gggcctgcaa accattgccg cgcctgtcgt
     2461 gacggctctg ctttatctgc ttattttcgc ccatgtgctt gaaggccggg ttcaggtgta
     2521 tggcaccgtg ccgtacacct cgtttctgat tcccggcctg atcatgatgg ccatgttgca
     2581 aaacgccttt gcaaatccgt cgtcctcgct gatccagagc cgcattaccg gcaatctggt
     2641 cttcattctg ctgccgccat tttcgcaccg cgaaattttc ggcgcgtata tgctggcgtc
     2701 catcgcacgc ggcgtgtgcg tcggcgtgtg cgtctggctg gttgccctgt tttttgtcgc
     2761 gctgcccgcc gccaatccgg tctggatcgt cgtcttcgcg atactgtcct gcggcatcat
     2821 ggccactctg ggaattatcg cgggactctg gtcggaaaaa ttcgatcagc ttgccgcgtt
     2881 tcaaaatttt ctgattgtgc ccgccacgtt cctgtcgggt gtgttctact ccatacacag
     2941 cctgccgcct ttttggcaag ccgtatcgcg ctggaacccc attttctata ccatcgacgg
     3001 tttccgttac ggctttttct cggcctccga tgtgtctccg tggcaaagcc tggccgtcgt
     3061 gacggttgta ttcattgtcc tcagcggctt tacccttcgc cttctggcca gcggttataa
     3121 actccggagt taattcccat gcaacccacg cccgaacaag ttcgccaata catcgccgat
     3181 catctaagct gcgaattcat cgacgtccgc ggcgacggtg cgcacttcga agcccttatc
     3241 gtcagcagcg ctttcgaagg caagcgcctg gtcgcacgcc accaactggt ctacgcggcg
     3301 ctgggcgaac gcatgaaagc cgaagtgcat gccctgtcga tgcgaaccct gacccccgcc
     3361 gaacaccagg ccagcctgaa tggataagct tttcatcaac ggcggcca
//
LOCUS       DATCXU010000446         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_988489,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000446 DATCXU010000000
VERSION     DATCXU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988489"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="mutM"
                     /locus_tag="VIR04_09975"
     CDS             <1..697
                     /gene="mutM"
                     /locus_tag="VIR04_09975"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY9461119.1"
                     /translation="ITGQVVLSCQRRGKYLLINFSNGTQIIHFGMSGSLRRVPMDEPR
                     RKHDHVEWIFARARFLLHDPRRFGAVLWHEASDGPIMAHPLFARLGIEPFDPLFTPKY
                     FHERLQGKTMTIKQVLLAGHIVVGVGNIYASEALFQARINPLLDAGKLSLNRCKRLID
                     SIQVTLRQALDSGGSTLRDYVNAQGKPGAYFEHHAAVYEHAQQPCRVCNTPIRRIVQG
                     QRATYFCPKCQRK"
     gene            complement(735..2003)
                     /locus_tag="VIR04_09980"
     CDS             complement(735..2003)
                     /locus_tag="VIR04_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY9461120.1"
                     /translation="MRYRSGMNYRDPFFYLLTQVVGITALLGWLAYRIQYSDLDVAIS
                     AYFHNSARYTALLQHDLALEALGHFLLWALPLTGAILSGAAALFGARFKALQPARVVL
                     WTLLAIFCLVPVVLVGLRHYTTLPGGYAVSGYVLFGLYFAGWALDRARLRWWGLLIAV
                     LAGGMVGALFVLQDLHFASETIWSAALAWLLCSLFFYPLIVTRHREGLADISRYTLDE
                     IWANLAKVQVRRRNTLTAYGVLFVCLLPFISATWPPRSWVHLTIEWVGLTFILAAVLG
                     RCWCILYLGGHKGATLIQQGPYSVSRNPLYLFSMLAVTGIGAQSGSIVLGPILALFVY
                     AVFNNVIDEEERLLRKVFGPQFDEYCSRVPRFGPRFKHWKTDDSLLISMSGLGNTMRD
                     ALPYFLALPLFELIELGQSSGWLPVLIRLP"
     gene            complement(2097..3512)
                     /locus_tag="VIR04_09985"
     CDS             complement(2097..3512)
                     /locus_tag="VIR04_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY9461121.1"
                     /translation="MKLLNELVEMLGASYVLTGPQAESYNVDWRNRYFGKALAVAKPG
                     STQQVAQLVKWCQSHQIPIVPQGGNTGLCGGATPDSSGTAIVVSTTRLNRIRSIDTDN
                     DTMVVEAGCILQSAQQAALQADRLFPLSLAAEGSCTIGGNLATNAGGTQVLRYGNMRE
                     LVLGLEVVTAEGEIMHGLRGLRKDNTGYNLRDLYVGSEGTLGIITAATLKLYPVPVAQ
                     RTALLGMRSIAEAVAVLSIARKEFGAALTGFELIAGNCLKAVVTCFPQQRVPFQGASA
                     ELPWYALLELSDSESAEHARARFEKVVGEIIEGDLVVDAAIAESVAQSKALWHLRESI
                     PLAEKELGKSIKHDVSIPVSKMAAFVEQTNAVLQASFPGIQHIIFGHLGDGNLHYNVS
                     RGDSYTEADLLARQADIYRLVHESVRQFSGSISAEHGVGQLKRDALPRYKDPVEMVLM
                     RRIKQALDPHGLMNPDKLLVR"
BASE COUNT          923 a         1145 c          966 g          719 t
ORIGIN      
        1 aatcaccggc caggtcgtat taagctgcca gcgccgcggc aaatatctgc tcatcaactt
       61 tagcaatggg acacaaatca tccattttgg catgtcggga tcattgcgcc gcgtccccat
      121 ggacgaacca cgacgcaaac atgatcacgt tgaatggatt tttgcgcgag cccgcttttt
      181 gctgcacgac ccgcgccgct ttggcgcagt cttgtggcac gaagccagcg acggacccat
      241 aatggcgcac ccgctatttg ccaggcttgg catcgagccg ttcgaccctt tattcactcc
      301 caagtatttt cacgagcgtt tgcaaggtaa aacgatgacc atcaagcaag tccttcttgc
      361 cggccacatc gttgtgggcg tgggcaatat ttacgcgtcc gaagcgctat ttcaggcccg
      421 aatcaatccg ctgctcgacg ccgggaaact atcgctaaat cgctgcaagc gcctgattga
      481 ttccatacag gtcactctgc gccaggcgct cgattcaggc ggcagcacac tgcgcgatta
      541 tgtcaacgca caaggcaagc cgggcgccta ttttgaacac catgcggccg tgtatgaaca
      601 tgcgcaacag ccctgccgcg tgtgtaacac gccaatccgg cgcatcgtgc aaggtcagcg
      661 cgccacctat ttttgcccga aatgccagcg gaaatagcgc acgattgcgc cagcgcccag
      721 acttggccag cagcttacgg caaacgaatc aataccggca accagcccga gctctgcccc
      781 agttcaataa gctcgaaaag cggcaaggcc agaaagtaag gcagcgcgtc gcgcattgtg
      841 ttgcccagcc cggacatcga gatcagcaaa ctgtcgtcgg ttttccagtg tttgaacctg
      901 gggccgaaac gcggaacccg cgaacaatat tcgtcaaact gcgggccaaa taccttgcgc
      961 aacaaacgct cttcctcgtc gatgacatta ttgaatacgg catatacgaa cagggccagg
     1021 atcggcccaa gcacgatgct gcccgattgc gcgccaatac ctgtgaccgc gagcatggaa
     1081 aacagataca gggggttgcg actaacggaa tagggcccct gctggatcaa cgtggcgccc
     1141 ttgtggccgc ccagatacaa aatgcaccag cagcgaccca gcaccgccgc caggataaat
     1201 gtgaggccta cccattcaat cgtcagatgc acccacgacc gcggcggcca tgtcgccgaa
     1261 atgaagggca acaggcacac gaacaatacg ccgtatgcgg ttaaagtatt ccgccggcga
     1321 acctgcactt ttgccagatt tgcccagatt tcgtccagag tatagcgaga gatgtcggcc
     1381 agcccctcgc gatggcgcgt cacgatcagc ggatagaaaa acaagctgca taacagccac
     1441 gccagcgccg ccgaccaaat tgtctcgctt gcaaaatgaa ggtcttgcaa aacaaacagc
     1501 gcgcccacca tgcctccggc gagcaccgca atcaagagac cccaccagcg aagccgggcg
     1561 cgatccagcg cccatccagc gaaatacagg ccaaacaaaa cgtaaccgga gacggcgtaa
     1621 ccgcccggca aagtggtgta atgcctgagc ccgacgagaa ccaccggaac cagacaaaaa
     1681 atcgcgagca aggtccacag aacaaccctg gccggctgca atgccttgaa tcgggcgcca
     1741 aacagcgccg ccgctccgga caaaattgcg cccgtcagcg gcaacgccca cagcaagaaa
     1801 tgaccgagcg cttcaagcgc aagatcgtgc tgcagcagag cggtataacg ggccgaatta
     1861 tggaaatacg cactaatggc cacgtcaagg tcggagtact gaattcgata ggccagccag
     1921 cccagcaacg cagtgatgcc caccacttgg gtaagcaagt agaaaaaggg atcacgataa
     1981 ttcatgccgg agcgataacg cataaaacca acacactaaa aaggcaataa gccgcgattg
     2041 taagctcgat tccaaatttc ggggcgccgg cttgaagcgc cctgggaaat cacgttctat
     2101 cgcaccagca atttatcagg attcatcagc ccatgagggt ccagcgcctg cttgatacgc
     2161 cgcatcaaaa ccatttcgac cggatccttg taccgcggca gcgcgtcgcg tttgagctgc
     2221 ccgacgccgt gctcggcgct gatcgatccg gaaaattgac gcacgctttc atgcaccagg
     2281 cgatatatgt cggcttgccg cgcaagcaag tcggcctcgg tataggaatc gcccctggag
     2341 acgttgtagt gcaaattgcc gtcgcccaga tgaccaaaga ttatatgctg aatgccggga
     2401 aaactggctt gcaacaccgc attggtctgc tcaacgaatg cagccatttt cgacacggga
     2461 attgatacat cgtgcttgat gcttttcccg agctcttttt cggccagcgg aatactttcg
     2521 cgcaagtgcc aaagcgcttt gctttgcgcc acgctttcgg caatcgcggc gtcaaccacc
     2581 agatcgccct cgatgatttc tcccaccact ttctcgaaac gggcacgcgc gtgctcggcg
     2641 ctttcgctgt cagacagttc gagcagtgca taccatggca gttcggccga agcgccttga
     2701 aatggcacgc gctgctgcgg aaagcacgta accacagcct tcaggcaatt tcccgcaatc
     2761 agctcgaacc cggtcaatgc tgcgccgaat tctttacgcg caatggataa aaccgccacc
     2821 gcttcggcga tcgaacgcat gcccaataat gcggtgcgct gagcgaccgg caccggatac
     2881 agtttcagtg tggcggccgt gataattcca agcgtgcctt cgctgccgac ataaagatcg
     2941 cgcaggttgt aacccgtatt gtctttgcgc agaccgcgca agccatgcat gatttcacct
     3001 tcggccgtca ccacttcaag gcccagcaca agttcacgca tattgccgta acgaagcacc
     3061 tgcgttccac ccgcgttggt cgccagattg ccgccgatcg tgcaactgcc ctcggcggcc
     3121 aggcttaatg gaaaaaggcg atccgcctgc agcgccgcct gctgggcgga ctgcagaata
     3181 cagccggctt cgacaaccat ggtgtcgtta tccgtgtcga tgctgcgaat tcgattcagt
     3241 cgagtcgtgg acacgacgat ggcagtgccg ctggaatcag gtgtggcgcc gccgcacaag
     3301 cccgtattcc cgccttgcgg cacgatcggt atttgatgag attgacacca cttgacgagc
     3361 tgcgcgacct gctgggttga cccaggtttg gctacagcca gcgccttgcc aaaatagcgg
     3421 ttacgccaat caacgttgta ggactcggcc tggggcccgg tcaggacata cgaagcaccc
     3481 agcatttcaa ccaattcatt caacaacttc attgctcacc cttataacgt ccggcccatt
     3541 ggccgcacat gacagactat ccattttaat cgccctttcg ccgcattgcc taaccggctc
     3601 agggcgcaac aatgcatgca atcagaatcg ccgataatac agactgttca tttcatggcg
     3661 ccgaaaaaaa ctactgtgac gattattgat ttaccacccg acgctgtctt caaggcctac
     3721 gacattcggg gtacgacgcc cgatcaactt act
//
LOCUS       DATCXU010000447         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_762192,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000447 DATCXU010000000
VERSION     DATCXU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762192"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VIR04_09990"
     CDS             complement(<1..398)
                     /locus_tag="VIR04_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9461122.1"
                     /translation="MNYDFDWTVISDNYGVLLDGAAMTVMVALITIVTSTVLGLFIAL
                     LRMSRWKVLRYSAIAYIEFFRTIPFLVQLFWVYYAIPEIFNVSLPSLSAGILALTLDV
                     AAFTAETFRAGLQSVGRGQYDAARALGMNPV"
     gene            complement(400..1065)
                     /locus_tag="VIR04_09995"
     CDS             complement(400..1065)
                     /locus_tag="VIR04_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9461123.1"
                     /translation="MGHIIEILSNYGDFLLRGVLLTLQASFLGSVFALIFGILIGIAR
                     VYGGKRLSAVLGVPVDIFRAIPLLVIVIWTFYALPVFLNVFLTPMIAGVLSLTVRYAA
                     TISEAVRGGILSIAPGQALAGSALGITKSRVIVRIVLPQALIRMIPPVTSQIVSLVKN
                     SSLLYVIAVTELMRQAETLNAQLAQPLAIFTIIAGIYFVIAYPITVISEIIYKRLYSR
                     AAN"
     gene            complement(1084..1701)
                     /locus_tag="VIR04_10000"
     CDS             complement(1084..1701)
                     /locus_tag="VIR04_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461124.1"
                     /translation="MQAEKVATALGVKLVAVPVSHDTKVTAVQTGQVDMTIAPLDVTD
                     ARKKVINFATNSVDGQCFVALKSNAKIRSLDDLKKPGIKVAIPLGGSQQQLIPTEYPQ
                     MKIMPQMLLPGVNWATASTLNGTTDVTIFPSAQVYLLLKAYSDKFNVIPDAEKCLSHP
                     VLPIAAGWGIQKGDPVFVKFVQGILDASQPAFDEQFVKMAKELGK"
     gene            complement(2005..2700)
                     /locus_tag="VIR04_10005"
     CDS             complement(2005..2700)
                     /locus_tag="VIR04_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9461125.1"
                     /translation="MDGINLGITRGELLCIIGPSGSGKSTLLRCMNRLEDIDSGKIIF
                     HEFEADGAPMDSVKLRRRIGMVFQHFNLFPHMTALENVMSGPRFALNKSKEEAAEKSK
                     ELLERVSLGAKLHSYPSELSGGQQQRVAIARALALDPEIMLFDEVTSALDPELVGEVL
                     AVMKRLAEDGMTMVVVTHEMAFAEKFASRVAMFDAGKIIETGSPQQIFRNASNPRTRT
                     FLSQVHLDDLLGS"
     gene            complement(2961..>3751)
                     /gene="rfbD"
                     /locus_tag="VIR04_10010"
     CDS             complement(2961..>3751)
                     /gene="rfbD"
                     /locus_tag="VIR04_10010"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY9461126.1"
                     /translation="DICDADRIGSFLDATPVDAVVNAAAYTAVDAAESNGQQAERVNA
                     VAPGLLAQACRQRNIWMLHISTDYVFDGKKSEPYREDDAVNPLSVYGKTKLAGEQAVL
                     RASPNAAILRTSWVFSPFSRNFLKTMLAMAQSRTELNIVNDQFGGPSYAGDIAAVLLR
                     LIDQHFNASPVAAGIYHLAGTPHVSWLDFSNNIFKQATELGLLAQAPKIHGIPSSSYP
                     TPAPRPANSRLSNAKLEKLLGDLDCDWRLGVRESLLYLKAKNAP"
BASE COUNT          830 a          996 c         1010 g          915 t
ORIGIN      
        1 acagggttca ttccaagcgc tcttgccgcg tcgtactggc cgcgacccac cgactgcagc
       61 ccggcgcgga acgtttccgc cgtgaacgcg gctacgtcca gggttaatgc cagaattccg
      121 gctgacagcg atggcagcga cacgttaaaa atttcgggga ttgcgtaata gacccagaag
      181 agttgaacca gaaacggaat agtgcgaaaa aactcgatat acgcgatggc gctgtaacgc
      241 aagaccttcc atcggctcat gcgaagcaac gcgataaaca atcccaatac cgtgctggtt
      301 acgatcgtaa tgagcgcgac cattacggtc attgccgcgc catccagcag cacgccgtaa
      361 ttgtcgctaa tgaccgtcca gtcgaagtcg taattcatgt caattcgccg cgcggctgta
      421 aagccgtttg tagataattt cggatatgac tgtaatggga taggcgatga cgaaatagat
      481 tccggcgatg atggtaaaaa tcgccaacgg ttgcgccaac tgggcgttaa gcgtttccgc
      541 ctggcgcatc agctctgtga cggcgatgac atagaggagt gaagagtttt tcaccagaga
      601 cacgatctgc gatgtcaccg gtggaatcat gcgaatcagt gcttgcggaa gaacgatacg
      661 aacgatcact ctgctttttg ttattcccag tgcgctgccg gccagcgcct ggccgggcgc
      721 gatcgacaga atgccgcccc gaactgcctc gctgatggtg gctgcgtatc gaaccgtcag
      781 gctcaatacg ccagcgatca tgggtgtaag gaagacattc aaaaatacgg ggagcgcata
      841 gaaggtccag atcacgatga ccagcaacgg tattgcgcga aatatatcga ctggcacccc
      901 caatacggcc gaaagccttt tcccgccata tactcgcgca atacctatca aaatgccgaa
      961 gataagcgcg aacacgctgc ccaagaagct tgcctgcagg gtgagaagca ctccgcgaag
     1021 caagaagtct ccgtaattgg agagtatttc tatgatgtgt cccacgcctt tggccttgtc
     1081 cggttacttg cccagttctt tcgccatttt tacaaattgc tcgtcgaagg caggctggct
     1141 ggcgtccaga attccttgta cgaacttaac aaacacaggg tctccttttt gaatccccca
     1201 gccagcggcg atcggcagca ccggatgcga caagcatttt tctgcatcgg ggatgacgtt
     1261 aaacttatcc gaatacgcct tcagcagcag gtacacttgt gccgacggaa aaatcgtgac
     1321 atcggtcgtg ccattcagcg tggaagcggt tgcccagttc acgccgggaa gcaacatctg
     1381 cggcatgatt ttcatctgcg ggtattcggt cggaatgagc tgttgctggg aaccgccaag
     1441 cggtattgcc acttttattc ctggtttttt cagatcatcg agactgcgaa ttttcgcgtt
     1501 cgatttgagc gcaacgaagc attggccatc gacggagttg gtcgcgaaat tgattacctt
     1561 ctttcgcgcg tcggtcacat caagaggcgc tatcgtcata tccacttgcc cggtctggac
     1621 cgcggttacc ttggtgtcat gactgacggg gacggcaacg agctttaccc caagtgcagt
     1681 tgcgactttt tcggcctgca tccacgtcgg tccgtaccat ggttgctttg ggttggcggg
     1741 attttcaccg acccacggaa gttgggataa cacccccact ttcaaacttc cacgcgactt
     1801 gatgcggtca atggtcggac tgctcgggac ggcgctggcg ctggcggtgg ccgccttcga
     1861 cgccgattcc gcataggtgt ttagcgccag cgttgaagcc atcgccattc cgactgcaag
     1921 caaacttttt ttccacatgg ttttcatttt ccagcctctt aatcaatcgt tggggttaac
     1981 gggggggtta ttgggacccg agcatcaact tcctaaaagg tcgtcaaggt gtacttggct
     2041 aagaaaggtt cgggtgcgtg ggttgctggc atttcgaaat atttgctgcg gggaccctgt
     2101 ttcgatgatt tttccggcgt cgaacatggc aaccctgctg gcgaatttct ctgcgaacgc
     2161 catttcgtgc gttactacca ccattgtcat tccgtcctcg gccagccttt tcatgacggc
     2221 cagaacctcg cccacgagct cagggtcaag tgccgaagtg acttcatcga aaagcatgat
     2281 ctcggggtca agcgcaaggg cgcgcgcgat ggcaacgcgt tgctgctggc cacctgatag
     2341 ttcggaagga taactgtgca gttttgcgcc aagtgacacg cgctccagca gttccttcga
     2401 cttttcagcc gcttcttctt ttgatttgtt caacgcgaat cgtgggcccg acatgacgtt
     2461 ttcaagggcg gtcatgtgag ggaaaagatt gaaatgctga aagaccatgc caattctgcg
     2521 acgcagcttt accgagtcca tcggtgcgcc atcggcttcg aattcgtgaa aaattatttt
     2581 cccgctgtcg atgtcttcca gcctgttcat gcaacgaagc aaggtggatt ttccggaccc
     2641 gctgggccca ataatgcata gcagctcgcc ccgcgtaatt ccaaggttta tgccatccaa
     2701 gattcgaact ttgccatatg acttttgcac atcgcgacat tcgagtacaa acgaatcccc
     2761 catcgcactt cctggttgta aggtttttag tagtcgccgg gtcttgcgac tgcggcgcag
     2821 gtgtctatag aatgcggtac ctgcgaatac tagcatcccg atagtcgttt tacaatggga
     2881 caatcttcgg ttatttttgt ataaaagaag taatttctct attctgaaaa gtagtaaaac
     2941 gcctttttca agcagtaatg ctacggcgcg tttttcgctt tcagataaag tagcgattcg
     3001 cgcacgccaa gccgccagtc gcagtccagg tcgcccagca gtttttccag cttggcgttg
     3061 gacaggcgag aattcgccgg tctgggtgcg ggtgttgggt aggacgaaga ggggatcccg
     3121 tgaattttgg gtgcctgcgc cagtaggccc agttcggttg cctgcttaaa aatattgttg
     3181 gaaaaatcca accagctaac gtgcggcgtt ccagcaagat ggtaaatgcc ggccgccacc
     3241 gggctggcgt tgaagtgttg gtcgatcaac cttaacagca ctgcagctat atccccggcg
     3301 taacttggcc cgccgaattg gtcattcact atgttcaatt cggtacggct ttgcgccata
     3361 gccagcatgg tcttcaggaa attgcggctg aatgggctaa acacccaact ggttctcagt
     3421 atcgccgcat taggcgaggc ccgcagcaca gcttgttcgc cggccagttt tgttttgccg
     3481 tatacgctta gcggatttac ggcgtcgtct tcacgatacg gctccgattt tttaccgtcg
     3541 aagacgtaat ctgtcgatat gtgcagcatc cagatgtttc gctgcctgca cgcttgcgcc
     3601 agtagcccgg gagcgacggc attgacgcgt tcggcttgtt gcccgttgga ctcggcggcg
     3661 tcgacggctg tataggcggc ggcgtttacc acggcgtcca ccggtgtggc gtccagaaat
     3721 gacccgatcc tatccgcatc gcaaatatcc a
//
LOCUS       DATCXU010000448         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_756050,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000448 DATCXU010000000
VERSION     DATCXU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756050"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..990
                     /locus_tag="VIR04_10015"
     CDS             172..990
                     /locus_tag="VIR04_10015"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461127.1"
                     /translation="MDQAAQSTNTVLDARDIELSYRRPNKQINTVLRDFSMTLAAGEI
                     IALLGPSGVGKSSLLRVLAGLQTPDKGTVLIKGEPITGPHPRLGFVFQDPSLLPWLSL
                     EENVGFGLDFKHQPAITKAEKIRRVNAAIAEVDLQNAVERHPHELSGGMAQRTALARS
                     LVRQPEILLLDEPFSALDEITRGEMQALLQQITARHKTSAILVTHDIDEALILADRLL
                     LIGLYPGRLIGQWHIDLPHPRDEVAPELTLMRVEITQTLREARRAVSASKHLNS"
     gene            999..2201
                     /locus_tag="VIR04_10020"
     CDS             999..2201
                     /locus_tag="VIR04_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461128.1"
                     /translation="MKKNAAPAPSLNARRQFLKLSTLFTVAGALPLLQTYTRAMAAEP
                     DAPVKIGYLPITDATALLVAHHNKLFENQGLRVEKPRRFRSWSQIVEAFLSGQVNVVH
                     LLMPMTVWSRYGSKAPAKVVAWNHMEGSSLTVLPEVNHLSDLGGKTVAIPFWYSIHNV
                     VLQHVLKSQGIESISDGQPGPKQVKLVVMSPGDMLPALGTKQIAGYIVAEPFNALAEI
                     LKVGKIWRFTGDVWKNHACCVVTMHEHDLEQRPEWSQKVVNGIVQAQAWTLDHRDEAA
                     HILAKDNPAQYTPHTHETLARVLAPDAEQAAQYEASGAIKHADWHEQRIDFQPYPFPS
                     YTEKLVEMLKTTYVSGRNEFLQTLDPKFVARDLVDDRFVKKAIADLGGMSKFGLPDSF
                     IREETITT"
     gene            2215..3003
                     /locus_tag="VIR04_10025"
     CDS             2215..3003
                     /locus_tag="VIR04_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461129.1"
                     /translation="MSNRLKPVRARTRLTLSLTGLCTLVLMWALGILWLKQSVSLASL
                     FSPTQTFGSLYELIVQNQLTQHTLISLKRVVVGLFWALVLGVPIGLAVGLSRALESST
                     SSAFQFLRMISPLSWMPIAVMLFGIGDAPIYFLLTFAAIWPIILNTSAGVRQLDPKWL
                     MLAQSLSATRREILFKIVIPGALGHILTGLRLSIGIVWIILVPCEMLGVSSGLGYFVL
                     DTRDRLAYSELMAVIVLIGALGFMLDSVAQKLYQYWMHAKPTGM"
     gene            complement(3021..>3300)
                     /locus_tag="VIR04_10030"
     CDS             complement(3021..>3300)
                     /locus_tag="VIR04_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEY9461130.1"
                     /translation="NAWGGAYHRRDAYARPPTGLIEPRAPRGLTVGVMVGDQHFRIGQ
                     GVRHAKFGDGTIISLSGMGQDAQARIQFRDAGAKTLALGVAKLEIIAG"
BASE COUNT          732 a          980 c          924 g          664 t
ORIGIN      
        1 gcgccatccg gctcagcgcc gcttgcgcga agcggtattc gtggccattg tcacgccagc
       61 gctaaagcat ttacgcaaag aaatccacga cattgagcac gcagccaatc aacacgccgc
      121 cgctgaagtg ttcgcgcgcg atccggcgcc ctttcaaact agggcggcat aatggatcaa
      181 gccgcgcaaa gtacaaacac ggtgctcgat gcgcgcgaca tcgagctcag ctacaggcgc
      241 ccgaacaagc agatcaacac agtgctgcgc gacttttcga tgactctggc cgctggcgaa
      301 ataatcgcct tgcttggacc cagcggtgtc ggaaaatcgt ccttgctgcg tgtgctggct
      361 ggtttgcaga cgcccgacaa aggaacagtg ctcattaaag gcgagcccat tacgggcccg
      421 cacccgcgcc tgggttttgt atttcaggac cccagcttgc tgccgtggct gtccctggaa
      481 gaaaacgtcg gcttcgggct tgattttaaa caccagccgg cgatcaccaa agccgaaaag
      541 atacggcggg tcaacgctgc gattgcggaa gtcgatctgc agaatgccgt ggaacgccat
      601 ccccatgaat tgtcaggcgg tatggcgcaa cgcacggcat tggcgcgcag cctggtccgc
      661 cagccagaaa tcctgcttct ggacgaacca ttcagcgcgc tggacgaaat tacacgcggc
      721 gaaatgcagg cgctgctgca acaaatcacg gcgcggcaca agacatcggc aattctggta
      781 actcacgaca tcgacgaagc gcttattttg gcggaccggc tgctgctgat cggtctttat
      841 ccggggcgtt tgatcggtca gtggcacatc gatctgcccc acccgcgcga cgaagtggcg
      901 ccggaactga cgctaatgcg cgttgaaatc acacagacgc tacgcgaagc gcgccgcgct
      961 gtatccgcat ccaaacatct aaattcctga agaagcccat gaaaaaaaat gccgcacccg
     1021 cgccgtcact gaacgcccgg cgccaatttc ttaaactatc cacgcttttc accgtcgccg
     1081 gcgcgttgcc gctgctgcaa acctatacca gggcaatggc ggccgaaccc gacgctccgg
     1141 tgaaaatcgg ctatcttccc atcaccgatg ccaccgcgct attggtggcg catcacaaca
     1201 agctctttga gaatcaaggc ctgcgcgtcg agaagccacg ccgctttcgg agttggtcgc
     1261 aaatcgttga agcgttcttg agcggccagg tcaacgtggt ccatttgctc atgccaatga
     1321 ccgtgtggtc gcgctacggc agcaaagccc ccgccaaagt cgttgcctgg aatcacatgg
     1381 aaggatcaag cctgacggtt cttccggagg tcaatcattt aagcgacctg ggcggcaaga
     1441 ccgtcgccat tcctttttgg tactcgatac acaatgtcgt gctgcaacac gtcctgaaaa
     1501 gccaaggaat tgaatctatc agcgacggcc agcccggccc caaacaagtg aagctggtgg
     1561 tgatgtcccc cggcgacatg ctgccggcgc tgggcactaa acaaatcgcc ggatacatcg
     1621 tggccgaacc cttcaatgct ctggctgaga tactgaaagt cggcaagata tggcgcttta
     1681 ccggcgacgt atggaaaaat catgcgtgct gcgtcgtgac catgcacgaa cacgatctgg
     1741 agcaacgccc ggagtggtcg caaaaagtcg tcaacggaat cgttcaggcg caggcctgga
     1801 cgcttgatca tcgcgacgag gcggcccaca ttctggccaa agacaatccc gcgcaataca
     1861 cgccgcatac acacgaaacg ctggcgcgcg ttcttgcgcc agacgctgaa caggcggcgc
     1921 aatacgaagc atcgggcgca atcaaacacg ccgactggca cgaacaacgg atcgattttc
     1981 aaccttaccc tttccccagc tataccgaaa aactggtcga aatgctgaaa accacctatg
     2041 tatcgggccg aaacgaattc ctgcaaacgc tggatcccaa atttgtcgcg cgcgatctgg
     2101 tcgatgaccg gtttgtgaag aaggccattg cggatctggg cggcatgtcc aaattcggtc
     2161 tgcccgattc gtttatccgg gaagaaacaa ttacaacctg agaacggcgc accaatgagc
     2221 aaccgactaa aacccgttcg cgcgcgaacc cgcctgacct taagcctgac aggactttgc
     2281 acgctggtgc taatgtgggc gctgggaatc ttgtggctta aacaaagcgt ttcactggcg
     2341 agcttgttta gccccacaca gacgttcggc agcctgtacg aactgatcgt gcaaaatcaa
     2401 ttgacgcaac atacgctaat cagtctgaag cgcgtggtgg tcggtttgtt ttgggcgctg
     2461 gtgctcggcg tgccgattgg gcttgcagtc gggctgtcgc gcgcgctcga aagcagcacc
     2521 agcagcgcct ttcaatttct gcgcatgatc tcgccgctat cctggatgcc gatcgcggtc
     2581 atgctgtttg gcatcggcga tgcgcctatt tatttcctgc tgaccttcgc cgccatctgg
     2641 cccattatct tgaatacgag cgcgggcgtg cgtcagctcg atccgaagtg gcttatgctg
     2701 gcgcaaagcc ttagcgccac caggcgcgaa atattgttca aaatcgtcat acccggcgcc
     2761 ttgggccaca ttctgacggg cttgcgttta tcaataggga tcgtctggat catactggtt
     2821 ccttgcgaaa tgctcggcgt aagctccggc ctgggctatt tcgtgctgga cacgcgcgac
     2881 cgcctggcct attccgaatt aatggcggtt atcgtgctga ttggcgcgtt ggggttcatg
     2941 ctggacagcg tggcgcaaaa actttatcag tattggatgc atgccaagcc aacgggtatg
     3001 tagcaagcgc gctacgtgga tcagcccgcg atgatctcca gcttggccac acccagcgcc
     3061 agggttttgg cgcccgcatc gcgaaactgg atgcgggcct gggcgtcctg acccatgccg
     3121 cttaaactaa tgatggtgcc atcgccgaac ttcgcgtggc gcacaccctg gccaattcgg
     3181 aaatgctggt cgcccaccat aacgcccaca gtcagtccgc gcggcgcgcg cggttcgatc
     3241 aggccggtgg gtgggcgggc gtatgcgtcg cggcggtgat acgcgccgcc ccaggcattt
//
LOCUS       DATCXU010000449         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_914517,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000449 DATCXU010000000
VERSION     DATCXU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914517"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VIR04_10035"
     CDS             <1..653
                     /locus_tag="VIR04_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HEY9461131.1"
                     /translation="SGFDRGYCLMIGGEAAIVGHLTPIFGALSPGVDAAARTSGRSGA
                     PSSAEQGWLHCGPHGAGHFVKMVHNGIEYGIMAAYAEGLNILRNANAGARKRDVDAET
                     TPMRNPELYQYDLDLPEIAEVWRRGSVVGSWLLDLTAQALHGNADLTDYAGRVSDSGE
                     GRWTVNAAIDEGVPAPVISAALFARFSSRGNDDYANRVLSAMRKQFGGHDEKKEGA"
     gene            655..2064
                     /gene="zwf"
                     /locus_tag="VIR04_10040"
     CDS             655..2064
                     /gene="zwf"
                     /locus_tag="VIR04_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY9461132.1"
                     /translation="MVSLRNEKLASDALVVFGMTGDLAYKKIFPALYQMVKRDGLRVP
                     VVGVASSKMSAAQLRKRIADSIKESGKIDDRPALKKLTSLVCYVDGNYNDQTTFEKLK
                     AALAGAVHPTYYLAIPPSLFGTVIKGLAAANLSKGARVVVEKPFGRDLASARALNEEA
                     RSVFDDDAIFRIDHFLGKEAIMNILYFRFANSFPEPIWNRDCVASVQITLSESFGIEQ
                     RGGFYETAGCLRDVIQNHLFQIVALLAMEPPVHRGFSAVHAEKDKVFQAMRPLKADDV
                     VRGQYRGYRKEPHVARNSDVETFCALRLFIDSWRWEGVPWYLRSGKCLPETVAEILVQ
                     LKPPPAKLFADSQPATGRANYLRFRVSPCSAVALAARVKRSGKEFVGDQRELFLLDED
                     PNEESPYERLLSDAIAGDRSLFTSEHAIEAAWAVVDPVLTHHDKAIPYTPGTWGPRQA
                     DALIAADGTWHNPTLTEAS"
     gene            2061..2759
                     /locus_tag="VIR04_10045"
     CDS             2061..2759
                     /locus_tag="VIR04_10045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY9461133.1"
                     /translation="MTVSDDLVFLFDVDNTLLDNDHVTEDLRLHLIEQFGQKSASLYW
                     TLFEALRAELGYTDYLGALQRYRADVMNDPGLLRMSSFLLDYPFSDRLYPGAVQVLAR
                     LGQVGRTVILSDGDVVFQPRKIQRSGLWDAVDGRVLVYIHKEKMLDAIVERYPARRYV
                     MIDDKLRILAAMKTVLGDRLTTVFPRQGHYALDSENTVLYPAADITIEHIGDLIHTSL
                     SVFESSVSASAHKE"
     gene            2766..>3319
                     /locus_tag="VIR04_10050"
     CDS             2766..>3319
                     /locus_tag="VIR04_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY9461134.1"
                     /translation="MNATRELQGVGQSLWLDNITRGLLDDGILEHYIKDLSITGLTSN
                     PTIFQKAIESTHLYDSAIHQKTLAGESSETVFFGLAIEDLQRAADLFRPVHEATKGVD
                     GWVSLEVSPLLASDTAGTIKAAVQLHALAARPNLFIKIPGTRAGVAAIEESIFRGVPI
                     NVTLLFSSEQYVAAASAYMRGIERR"
BASE COUNT          695 a          879 c          966 g          779 t
ORIGIN      
        1 catccggatt tgatcgcggc tattgtctca tgattggcgg cgaggcagcc atcgtcgggc
       61 atttgacgcc aattttcggg gcgctgtccc ccggcgttga tgccgcggcg cgcacgtcag
      121 ggcgcagtgg cgcaccgtcc agcgccgagc aaggctggct gcattgcggg ccgcacggcg
      181 cgggtcattt tgtgaagatg gttcacaacg gcatcgagta cggaataatg gctgcgtacg
      241 ccgaaggcct gaacattttg cgcaatgcga atgcgggagc gcgcaagcgg gatgtcgacg
      301 ccgaaactac gcccatgcgc aatcccgagc tttatcagta cgatctggat ttgcccgaaa
      361 ttgccgaggt gtggcgccgg ggcagcgtgg tcggctcgtg gttgctggat ctgacagcgc
      421 aagccttgca cggcaatgcc gatttgaccg actacgcggg acgcgtttcg gattctggcg
      481 agggccgctg gaccgttaac gcggccattg acgaaggtgt gccggcccct gttattagcg
      541 ctgccttgtt cgcgcgtttt tcatcgcgag gcaacgatga ttacgcaaac cgcgtccttt
      601 cggccatgag gaaacagttt ggcggccatg acgagaaaaa ggagggcgct tgaaatggtc
      661 agtctacgca acgaaaagtt ggcatccgac gcgcttgtgg tgttcggcat gacgggcgac
      721 ctggcttaca aaaaaatttt tccggcgctt tatcagatgg tcaagcgtga tggtcttcgt
      781 gtgccagtgg tgggtgtggc gtcttcaaaa atgagcgctg cgcaattgcg aaagcgaatt
      841 gccgacagca tcaaagaatc gggaaagatc gatgacaggc cagcactgaa aaaactaacg
      901 tcgctggtgt gctacgtgga tggcaattac aacgaccaga caacttttga aaagctgaaa
      961 gcggcgctgg ctggcgccgt gcatcccacc tactatttgg ccatacctcc atcgcttttt
     1021 ggaacagtga tcaaagggct tgccgccgcg aacctgtcca agggagcgcg ggttgtcgtc
     1081 gaaaagccgt ttggccgcga cctggcatcg gcccgtgcgc tcaatgaaga agcgcggtca
     1141 gtgttcgacg atgacgcgat ttttcgcatc gaccactttc ttggcaagga ggcgatcatg
     1201 aatattctgt actttcgctt cgccaattcc ttcccggagc caatctggaa tcgggattgc
     1261 gtggccagcg tgcaaattac gctttccgag tcctttggca ttgagcagcg cggcggcttt
     1321 tacgagacgg cgggctgcct gcgcgatgtc attcaaaacc atttattcca gatcgtcgcc
     1381 ttgcttgcca tggagccgcc cgtgcaccgg gggttttcag ccgtgcatgc tgaaaaggat
     1441 aaggtgtttc aggccatgcg cccgttaaaa gccgacgatg tcgtgcgcgg gcaatatcgc
     1501 ggctatcgca aggaacccca tgtagccagg aattcggatg ttgaaacatt ctgcgcgtta
     1561 aggcttttta tcgattcgtg gcgctgggaa ggcgttccct ggtatttgcg ttcgggcaag
     1621 tgtctgccgg aaacagtggc cgagatactt gtccaattga agcctcctcc cgcaaagcta
     1681 tttgccgatt ctcagcccgc caccggtcgt gccaattatc ttcggtttcg ggtttcccca
     1741 tgctcggctg tggcgttggc ggcgcgggtc aagcgctcgg gcaaggaatt cgtcggcgat
     1801 cagcgcgagc tctttttgct tgatgaggat cccaatgaag aatctcctta tgaaagactt
     1861 ctgtcggacg cgatcgcggg tgaccggtca ttgttcacca gcgaacacgc catcgaggca
     1921 gcctgggcag tcgttgaccc tgttttgacc catcatgaca aggcaattcc atataccccc
     1981 ggaacctggg gccccaggca ggccgatgcg cttatcgccg ccgatggcac atggcataac
     2041 cccacgctaa cggaggcatc atgaccgttt ccgacgacct ggtcttctta tttgacgtgg
     2101 acaacactct gctggacaac gatcatgtga ccgaggacct tcgcctgcac ttgatcgaac
     2161 agttcggcca gaaaagcgcg agtctgtatt ggactttatt cgaggctctg cgcgccgagc
     2221 tgggttacac cgactatctg ggcgccttgc aacgctatcg cgccgacgtg atgaacgacc
     2281 ctggcttgtt gcggatgtcc tcctttttgt tggactatcc tttctcggat cgcttgtatc
     2341 ctggcgcggt ccaggttctg gcgcgccttg ggcaagtggg ccgcactgtc attttgtccg
     2401 atggcgacgt ggtgtttcaa ccgcgcaaga tccagcgatc tggcttatgg gacgctgtcg
     2461 atggccgtgt cctggtgtat atacataaag agaagatgct tgacgcgatc gtcgagcgtt
     2521 atccggcgcg gcgctacgtc atgatcgacg acaaacttcg tattttggcg gcgatgaaaa
     2581 ctgttttggg cgaccggctg acgactgttt ttccgcgcca gggacattac gccctcgatt
     2641 ctgaaaatac tgttttatat cctgctgccg acattactat cgagcatatt ggcgatttga
     2701 ttcacaccag tttatcggta tttgaaagta gtgtctcagc cagtgcacac aaggagtagc
     2761 tagccatgaa cgcaacacgt gaacttcaag gggtgggcca aagtctgtgg ctcgacaata
     2821 ttacccgtgg gcttttggac gatggcattc tggagcatta catcaaggat ttatcgatca
     2881 ccggattaac gtcgaatccg acgatttttc agaaggccat cgaaagcacc catctttatg
     2941 acagcgccat tcatcaaaaa acgctcgcag gagaatcgag cgaaacagtg ttctttggac
     3001 tggccattga agatttgcaa cgggctgcag acctgtttcg tcccgtccat gaagccacca
     3061 aaggcgtcga tggctgggtc tcgctcgagg tttcccctct gctggcttcc gacactgcgg
     3121 gaacgataaa agccgctgtt cagttgcatg ctcttgcagc ccgacccaat ttattcatca
     3181 agataccggg aacacgggca ggagtggccg ctatcgaaga gtcgattttc agaggcgtac
     3241 cgatcaatgt gacgttattg ttttccagcg aacaatacgt ggcggcggcc agcgcgtata
     3301 tgcggggtat cgaacggcg
//
LOCUS       DATCXU010000450         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_548479,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000450 DATCXU010000000
VERSION     DATCXU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548479"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VIR04_10055"
     CDS             <1..187
                     /locus_tag="VIR04_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY9461135.1"
                     /translation="TGIYAHVRGESWRVQSRQPLVRGDHVRVLAMEDLTLHVEPIGAG
                     LDKPMNAVDKTGGHHVL"
     gene            177..971
                     /locus_tag="VIR04_10060"
     CDS             177..971
                     /locus_tag="VIR04_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY9461136.1"
                     /translation="MFYDFNVGLSAVVIIVALVAFSAVKILREYQRGVIFTLGRFTRV
                     AGPGMIIVIPGLQQMVRVDLRVVTMDVPSQDVISRDNVSVKVNAVLYFRVIAPEKAII
                     QVERYLEATSQLAQTTLRAVLGKHELDDMLSEREKLNLDVQKILDAQTDSWGIKVTNV
                     EIKHIDLNENMIRAIARQAEAERERRAKVIHAEGEKQAAQALMEAAAILAEQPSAMQL
                     RYLQTLTQVAGDKSSTLVFPVPVDLLSSLMAPKGSIQEPATPSIRA"
     gene            complement(958..1443)
                     /locus_tag="VIR04_10065"
     CDS             complement(958..1443)
                     /locus_tag="VIR04_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY9461137.1"
                     /translation="MGSMAYERAKPWAISQWLNTPNALTVQSLRGYVVVLHVFQMLCP
                     GCVSHGIPQAKAIHAAFPPDKVKVIGLHSVFEHHDAMTPVALKAFAHEYRLSFPIGID
                     QPDPKNAIPVTMRDYGLEGTPTLILIDKSGYIRLQHFGQIDDLRVGAVLGQLLTEPMP
                     E"
     gene            1594..>2194
                     /locus_tag="VIR04_10070"
     CDS             1594..>2194
                     /locus_tag="VIR04_10070"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461138.1"
                     /translation="MRSRHTSEPTDAHQSGNGEKGGARAAIAAHTRAPLTCLLSNGRY
                     HVMASNAGGGYSHWRNTALTRWQEDFTRDNLGTFIYVRDADDHDFWSSTLQPTCGSAA
                     NYRAAFSGAKAVFDSNVRNVATHTDITVCVDDDVELRRVTIVNHGDSRRALDLTSYAE
                     VVLDSPAKDDAHPAFSKLFVQTEIIPDALAIICHRRGSNA"
BASE COUNT          508 a          551 c          630 g          505 t
ORIGIN      
        1 cacaggtatt tacgcacatg tacgcggtga aagctggcgg gttcaaagcc ggcaaccgct
       61 cgttcgaggc gaccatgtca gggttcttgc aatggaagat ctgacattgc atgtcgagcc
      121 gattggcgcg ggactggaca aaccgatgaa tgcggtggac aaaacaggag ggcatcatgt
      181 tttatgattt caatgtgggg ttgagcgccg tagtcattat tgtcgctttg gtggcgttta
      241 gcgctgtcaa aatactaagg gaatatcagc gcggcgttat ttttaccctg ggccgattta
      301 cgagagtggc gggccccggc atgattatcg tcattcctgg cttgcagcag atggtgcgcg
      361 tcgatttgcg cgttgtcacc atggatgtgc ccagccagga cgtcatctcg cgcgacaacg
      421 tatcggtgaa ggtgaacgcg gttttgtatt ttcgcgttat tgcgcctgag aaggccatta
      481 ttcaggttga gcgttacctc gaggccacca gtcagttggc ccagaccaca ttgcgcgccg
      541 tattgggaaa acacgaactg gacgatatgt tgtccgagcg tgaaaagctg aatctggatg
      601 tgcaaaaaat tctggacgcg caaaccgatt catggggcat taaagtcacc aatgtcgaaa
      661 tcaaacatat tgatttgaac gaaaacatga tccgggcgat tgcgcgccag gcggaagccg
      721 aacgcgagcg gcgcgcaaag gtgatccacg ccgaagggga aaaacaggcc gctcaggcct
      781 tgatggaggc tgccgccatt ctggcggaac agccttcggc catgcaactg cgttatcttc
      841 aaacacttac gcaagtggcg ggggacaaaa gctcgacatt agtctttccc gtaccggtcg
      901 atttgctatc gagcctgatg gcacccaaag gatctattca ggaacccgcg acgccatcta
      961 ttcgggcata ggttcggtca gcaattggcc gagcacagct ccaacccgca aatcgtcaat
     1021 ttgaccaaaa tgctgtaaac ggatgtaacc cgacttgtcg atcaggatga gcgttggggt
     1081 accttccaga ccgtagtcgc gcatcgtaac gggaatggcg tttttgggat ccggttggtc
     1141 aatgccgatc gggaaactga ggcggtattc atgcgcgaat gcttttaatg ccaccggcgt
     1201 catggcgtcg tggtgttcaa ataccgaatg caagccaatg actttcacct tgtcgggtgg
     1261 gaaggcggca tgaatcgctt tggcttgtgg tatgccgtgc gatacacagc ccggacaaag
     1321 catttggaag acatgcaaaa cgacgacgta gccgcgcagg gactgcactg tgagtgcatt
     1381 tggcgtattg agccactggc tgattgccca aggttttgcg cgttcgtaag ccatactgcc
     1441 catcaaaggc tcccggttgt aaaaatgaca tgataggcgt attttcaaag ggcggcttga
     1501 acttacctct atgaggcggg tctagtcttt agttgatcaa tctattgtga gtgccaaata
     1561 gcgagtcctt aacttacgtc acggggtata aacatgcgtt cgcgccacac gtccgaaccg
     1621 accgatgcgc accaaagcgg aaatggtgaa aagggggggg cgcgtgccgc aattgccgct
     1681 catacgcggg cgccattgac gtgcttgtta tcgaacggcc gttatcacgt tatggcaagc
     1741 aatgccggtg gcggttacag ccactggcga aatactgcat tgacgcgctg gcaagaagac
     1801 ttcacccgcg ataatcttgg tacgtttatt tatgtgcggg atgccgacga tcacgatttc
     1861 tggtcgagca ctcttcagcc gacctgtgga tcggccgcca actaccgggc ggcattttcg
     1921 ggcgccaagg cggtctttga cagcaacgtt cgcaatgtgg cgactcatac cgatattact
     1981 gtatgcgttg atgacgatgt cgagctgcgc cgggtaacga ttgtcaatca cggcgattcc
     2041 cgcagggcgc ttgatttaac cagctatgcc gaggtcgttc tggacagccc cgccaaggac
     2101 gatgcccatc ccgctttcag caaacttttt gttcagactg aaatcatccc cgatgctctg
     2161 gccattatct gccatcggcg cggcagcaac gccg
//
LOCUS       DATCXU010000451         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_364187,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000451 DATCXU010000000
VERSION     DATCXU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364187"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..140)
                     /locus_tag="VIR04_10075"
                     /pseudo
     CDS             complement(1..140)
                     /locus_tag="VIR04_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(251..487)
                     /locus_tag="VIR04_10080"
                     /pseudo
     CDS             complement(251..487)
                     /locus_tag="VIR04_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042044074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            491..862
                     /locus_tag="VIR04_10085"
                     /pseudo
     CDS             491..862
                     /locus_tag="VIR04_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
     gene            complement(914..1339)
                     /locus_tag="VIR04_10090"
     CDS             complement(914..1339)
                     /locus_tag="VIR04_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY9461139.1"
                     /translation="MKITSTLSLMIALSASTLAFAQSGNMKNMNASAPAQSQNMDMKA
                     MGMQQGNMMDMSKMDKMGQTTAPKTETHAAVGVVKATDLAKSTVTVAHGPVASLKWPA
                     MAMTFAVKDKTLFDKLAVGNKVSIEFLQQGSDYVVTSVK"
     gene            complement(1368..>2345)
                     /locus_tag="VIR04_10095"
     CDS             complement(1368..>2345)
                     /locus_tag="VIR04_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY9461140.1"
                     /translation="RDAAARYGLNIADVQSYVSTAIGGENIGEVLDGLRRFPINVRYP
                     REIRDSVEKLRDLPILTERGAQIRLGDVTTIAIKDGPSMLKSENARLSGWVYIDIRGR
                     PLSAVVRDMQQVVAQEVKLPPGYSIAWSGQFEYLQRAAQKLKVVVPATLLIIFVLLYL
                     IFKRFDEAALIITTLPFALAGGVWLLWILGYNLSVADGVGFIALAGVASEFGVVMLLY
                     LKNAWDARVSRGNTTQADLLDAIREGAVLRVRPKAMTVAVIIAGLIPIMIGMGTGSEI
                     MKRIAAPMVGGMITAPLLSMFVVPAVYLLLRRRQCGQALSNDVTEHQVI"
BASE COUNT          456 a          667 c          650 g          572 t
ORIGIN      
        1 tcggccaccc acagccggtt cggtcggtct gcctcaaagt ggcgcttgac caagtcctgc
       61 gggcaggcgg ccgcgttgtc gggcacggtg gtacgcaccc gcttgccacg acgaacccct
      121 tcaatgccca gcctgcgcat ctgtcaacag cgatttaaat ctaatacagt ttttctccaa
      181 gcagcgattt aaaactaata cacccccgtg gccggcgagc gccggctttc tcctctattg
      241 aacccgcttt ttaatcagcc tcttgcacca tgccggcttg gcgttgatgc ttcagccgat
      301 agctctcgcc ggcgatgggc acgatgtggg cgtggtgcag caaccggtcc agcagcgccg
      361 ccgtcagcgt tgtgtcctgc gcgaaggtgg cgtcccactg cccgaacggc aggttgctgg
      421 tcacgaccag gctgcctcgc tcgtactgag cggcgatcac ctggaagaac agattggctt
      481 gctcacgatt ccctattcga tctccgcatt attgctgact ttggttttgc tttttgcgtt
      541 tcagggcgaa gccattattg cgcagccacc gatcattggg ctgcttgcgg taccaattct
      601 tattcaagtg ttttttaatt ccggcctggc ctacttgctc aatcgcaagc tgggtgtccc
      661 gcattgtgtg gcgggtccat ccagcctgat tggtgcgtcc aatttctttg agctggctgt
      721 ggccactgct attagtctgt tcggtttccg atccggagcc gccttggcca cagtcgtggg
      781 cgttcttatc gaggtgccgg ttatgctgtt ggtggtcaat gtcgtgaacc gctcgcaaca
      841 ctggtatgag gcaaaggctt aagcaaaact ttaacaggcg ctcgatacct gaatggaatg
      901 tagacgttgc cggctatttc acgctggtga ccacatagtc ggagccttgt tgcaggaatt
      961 cgatgctaac cttatttccg acggccaatt tgtcgaacag tgttttatct ttcaccgcga
     1021 acgtcatggc catcgctggc catttcaggc tggcgacagg gccgtgcgcg accgtaacgg
     1081 tgctctttgc aagatcggtc gctttcacca ctccgaccgc ggcgtgagtt tcggttttag
     1141 gcgctgttgt ttggcccatt ttgtccattt tgctcatgtc catcatgttg ccctgctgca
     1201 ttcccatcgc cttcatgtcc atgttttggg attgtgcggg cgcgcttgcg ttcatgtttt
     1261 tcatgtttcc ggattgcgca aaagcaagtg tggaagcgga tagggcaatc atcaaagaca
     1321 aagtgcttgt aattttcatg gtgttttcct ttttttgtga gggttggtta aataacttgg
     1381 tgttcagtga catcgtttga aagtgcttgc ccgcattggc ggcgacgcag taataggtag
     1441 accgctggca cgacaaacat cgacaggagc ggcgcggtga tcatgccgcc gaccatcggg
     1501 gccgcgatac gcttcatgat ttcggaaccg gtgcccatgc caatcatgat gggtatcagg
     1561 ccggcgatga tgacagcgac cgtcatggct ttgggccgca cgcgcagcac ggccccttcg
     1621 cgaatggcgt ccagcaagtc ggcctgggtt gtgttgcccc gggacacgcg agcgtcccag
     1681 gcattcttta ggtacagcaa catgacgact ccaaattcag aggcaacgcc agccagcgcg
     1741 ataaacccaa cgccatcggc cacggacagg ttgtatccca atatccataa caaccagacc
     1801 ccgccggcca gcgcgaacgg cagcgtggta ataatcagcg cggcttcgtc gaaacgcttg
     1861 aagatgaggt acagcagcac gaagataata agcaatgtcg ccggaacaac caccttcaat
     1921 ttctgtgcgg cgcgctgcaa gtattcgaac tggccggacc atgcgatgga atatcccggc
     1981 ggcagcttga cttcctgggc cacgacctgt tgcatatcgc gcaccaccgc gctgagcggc
     2041 cggcctcgga tgtcgatata gacccacccg gataggcgtg cgttctcgct ctttaacatg
     2101 gacggtccat ctttgattgc gattgtcgtc acatcgccca ggcggatttg cgcgccgcgt
     2161 tcggtgagta ttggcaggtc gcgtaacttc tcgaccgaat cgcgaatctc gcgcggatag
     2221 cgcacattga tcggaaagcg ccgcagccca tcaaggactt cgcctatgtt ttcaccgcca
     2281 atcgccgtag acacataact ttgcacgtcg gcgatattca ggccataacg agcggcggcg
     2341 tcccg
//
LOCUS       DATCXU010000452         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_166676,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000452 DATCXU010000000
VERSION     DATCXU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166676"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VIR04_10100"
     CDS             complement(<1..854)
                     /locus_tag="VIR04_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461141.1"
                     /translation="MMNSLADPTLPNATPTGASPRRTRMKAALQSRMAMGIGLLIILI
                     AVWLIGLAAGAGVEQLLSLTVWGIMLGGILALGGVGLTLCYGVLKFPNFAHGELVTIG
                     AYVTFAIVQVVPQSAPIWGFSFGWELLAGLLVAMPVTGAVGWLVDRSLYRRLRRGKSP
                     LVLFAMASLGMAFFLRSLIYLVWGSDFHFYYVGRANPALNLPWGIRVQADQLFVLGLA
                     LVLVILLYLLLAKTRIGKAMRATADNPDLAQIRGIDTERVIAWTWLISGGLAAAGGVM
                     FALASQLRP"
     gene            complement(862..1569)
                     /locus_tag="VIR04_10105"
     CDS             complement(862..1569)
                     /locus_tag="VIR04_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461142.1"
                     /translation="MPLLKIDKITGGYGDSEVLRQVSMSIDQNEIVAIIGPNGAGKST
                     LMKAVFGLLHPREGHIVFDGTEISQLAPHQIVKLGMCYVPQVSNVFTSLSVEENLEMG
                     AFALENGDVEERKEQVYGMFPRLKERRRQLVGKMSGGERQMVAMGSALMIKPKLLLLD
                     EPTAALSPKLVDEIFDHVARINKIGIAVMMVEQNARQSLQMAHRGYVLASGENRQEGT
                     GQELLDDPNVGRLYLGG"
     gene            complement(1550..2383)
                     /locus_tag="VIR04_10110"
     CDS             complement(1550..2383)
                     /locus_tag="VIR04_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461143.1"
                     /translation="MVEQLSPDPVAVAGADQNEVLLDVNDIAKNFDGLRAVDRCSFSV
                     RRGAITGLIGPNGAGKTTAFNIITGFIPPDSGRILFRGADITGFRPDQIFERKLCRTF
                     QIPREHSSMSVLENLMLVPTGQLGERVWNNWFRRNQVRRQEDQNREKALEVLEYLELT
                     HVQDEYAGNLSGGQKKLLELGRTLMVDPELVLLDEPAAGVNRTLLKSLVANIQYLARE
                     RGITFLLIEHDMNVVMSLCDPVIVMSEGRKLIEGTPDEVRSNQSVLEAYLGGQYAAVE
                     N"
     gene            complement(2530..3498)
                     /locus_tag="VIR04_10115"
     CDS             complement(2530..3498)
                     /locus_tag="VIR04_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9461144.1"
                     /translation="MWSGILFALTAGLIWGLVFIGPLLLPEYPAALQSVGRYLAFGII
                     AVPLAWLDRTRLHRFAVADWVEALKLAAIGNLLYYLCLASAIQRAGGPLPTMIIGTLP
                     VVISISANLRNAKRDGRIAWRRLAPSLLLIVIGIYCVNRVELDVLRNSAHVDLARYVS
                     GALFALGAVACWSWYALRNADWLRGHPDHNPRTWATAQGVATLPLALAGYVVLWGGMA
                     LGHSSFVMPFGPRPGFFIALMLIIGLFASWLGTLCWNEASQRLPTTIAGQLIIFETLA
                     ALAYAFLLRGRAPEPLTLLGIAFMIAGVVWALRIRPQPVPAKKHIG"
     gene            complement(3531..4748)
                     /gene="fabB"
                     /locus_tag="VIR04_10120"
     CDS             complement(3531..4748)
                     /gene="fabB"
                     /locus_tag="VIR04_10120"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY9461145.1"
                     /translation="MKRVVVTGLGIVSSIGNNKKEVIDSLKEGRSGIVFSQTYKDLGF
                     RSHVHGPVNIDLDSLIDRRTKRFMGDGAAFSYVAMQQAIDDAGLDESDISNFRTGLVM
                     GSGGPSTSNIVEAADLLREKGVKHVGPYRVPRTMSSTTTACLATPFKIKGVNYSISSA
                     CATSAHCIGHAMELIQMGKQDIVFAGGGEEEHWTMSVLFDAMGALSSKYNDAPTTASR
                     PYDATRDGFVISGGGGVLVIEELEHAKARGANIIAELTGYGATSDGYDMVQPSGEGAV
                     RCMQQAMATVTGKIDYINAHGTSTQVGDTRELEALRTVFGDGAVPPVSSTKSLTGHAL
                     GAAGVNEAIYSLLMMKENFLSASANIVQLDPGAQGIPIVRERRDNVTLNTVMSNSFGF
                     GGTNATLIFQRYN"
     gene            complement(4759..>5128)
                     /gene="fabA"
                     /locus_tag="VIR04_10125"
     CDS             complement(4759..>5128)
                     /gene="fabA"
                     /locus_tag="VIR04_10125"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HEY9461146.1"
                     /translation="DKGEVIAELDIRPDLWFFACHFKGDPVMPGCLGLDAMWQLLGFY
                     LCWLDHPGKGRALGVGEVKFSGQVLPTNKKVTYRINLRRVISRKLVLGIADAVMEVDG
                     LVIYKAADLRVGLFTSTKDF"
BASE COUNT         1135 a         1515 c         1388 g         1090 t
ORIGIN      
        1 ggacgcagtt gcgacgccag tgcgaacatg accccgccgg ccgccgccag gccgccgctg
       61 atgagccagg tccaggcgat gacgcgctcg gtgtcgatcc cgcgaatctg cgccagatcg
      121 ggattgtcgg ccgttgctcg catggctttg cctatacgtg tcttggccaa taacaggtaa
      181 agcagaataa ccagcacgag cgccaggccc agcacgaata gctgatccgc ctgcacacga
      241 attccccagg gaagattaag cgccggattg gcgcgcccca catagtaaaa atggaaatcc
      301 gacccccata ccagataaat aagactgcgc agaaaaaagg ccattcccag cgacgccatg
      361 gcaaacagca ccagcgggga tttgccccgc cgcaggcggc gatacagcga tcgatcaacc
      421 agccacccca ctgcccccgt caccggcatc gcgaccagca gacccgccag caattcccat
      481 ccgaacgaaa aaccccagat tggcgcgctt tgcggaacaa cctgaacaat cgcaaaggtg
      541 acataggctc cgatggtgac aagttcgcca tgcgcgaagt tggggaactt cagtacgccg
      601 tagcacagag tcagtccgac tcctccaagc gcaagaatgc cgcccagcat aatgccccag
      661 acagtcagtg agagcagttg ctctaccccg gcgccggccg ccagcccaat cagccatacc
      721 gcaatgagga taatgagcag cccaataccc atggccatgc ggctttgaag cgccgctttc
      781 atacgcgtac gccgcggcga ggcgccggtg ggcgttgcgt tcggcagtgt tgggtcggca
      841 agggaattca tcatggattg ctcatcctcc gagatagaga cgtccgacgt ttggatcgtc
      901 gagcagttcc tggccggttc cttcctggcg attttcgccg cttgccagca cgtacccccg
      961 gtgagccatt tgcaatgact gtctggcgtt ttgctcaacc atcatgacgg cgatgccgat
     1021 tttattgatg cgcgcgacgt gatcgaatat ttcgtcgacg agctttggcg acagtgccgc
     1081 tgtcggttcg tccaataaca gcagttttgg ctttatcatt agcgcactgc ccattgccac
     1141 catttgccgt tcgccgcccg acatttttcc cacgagctgc cggcgccgct ctttcagccg
     1201 cggaaacatg ccgtagacct gctctttacg ttcctccacg tcgccatttt cgagcgcgaa
     1261 cgcgcccatc tcaagatttt cctcgactga gagactggtg aatacattgg atacttgcgg
     1321 cacgtagcac atgccgagtt tgacgatttg atgcggcgcc aattgtgaaa tctcggtgcc
     1381 gtcgaaaaca atgtggccct cgcgcgggtg aagcaagccg aaaacggcct tcatcagggt
     1441 cgatttaccc gccccgttgg gtccgatgat cgcgacgatt tcgttttggt caatggacat
     1501 cgaaacctgc cgcagtactt cggagtcacc gtagccgccg gttattttgt caattttcaa
     1561 cagcggcata ttgccctccg agatacgctt cgagcacgct ttgattgctg cgaacctcgt
     1621 ccggcgtgcc ttcgatcagc ttacgcccct cgctcatgac aatgaccgga tcgcacaagc
     1681 tcatgaccac gttcatatcg tgctcaatca gcagaaacgt gatgccccgt tcgcgcgcca
     1741 ggtactggat attggcgacg aggcttttta gcaatgtgcg attgacgccg gccgctggct
     1801 catcgagcag caccagttcc gggtcgacca taagggtgcg ccccagttcc agcagctttt
     1861 tctgaccgcc cgaaagattg ccggcgtatt cgtcttgaac atgggtcaat tcaaggtatt
     1921 cgaggacttc cagcgccttt tcccgatttt gatcttcttg ccgccggacc tggttgcgac
     1981 gaaaccagtt attccagacg cgctcgccga gctgacctgt gggtacaagc atcaggtttt
     2041 cgagcacgct catgctgctg tgttcgcgcg gaatctgaaa tgtgcgacag agcttgcgct
     2101 cgaatatttg gtcaggccga aaaccggtaa tgtccgcgcc gcgaaacagg atccggccag
     2161 aatcgggagg gatgaagccg gtaataatat taaaggccgt ggtttttcct gcgccattgg
     2221 ggccgatcag tccggtgatg gcgccgcgtc gtaccgaaaa tgaacagcga tccaccgccc
     2281 gcagaccgtc aaaatttttt gcaatgtcat tgacgtcgag aagaacttcg ttctgatccg
     2341 cgccagcgac agccaccggg tccgggctta attgttctac catggttagt gaaccgtctc
     2401 ctgtgtgctt caggcttcct gcccagcggg cagcgcctgg ctttttctaa atcttaccat
     2461 agtagtttct ggcccagtcg caataaacac gctttggaag cgatcgttta cccttcaccc
     2521 tatgtgcttt cagccgatgt gttttttggc ggggacgggt tgcggcctta tccgaagcgc
     2581 ccacaccacg ccggcaatca tgaatgcaat gccaagcagc gtaagcggtt caggagccct
     2641 tccgcgcaat agaaatgcgt atgccagcgc tgccagggtt tcgaaaatga tcaattggcc
     2701 ggcaattgtg gtgggcagac gctgactggc ctcattccag cataatgttc cgagccagga
     2761 agcgaacagg ccgatgatta acatcagggc gatgaagaaa ccgggtctgg gtccgaaagg
     2821 catgacgaag ctgctatgac ccagcgccat gccgccccac agaacgacat acccggccag
     2881 cgccagaggc agcgttgcaa cgccctgagc cgttgcccag gtgcgcgggt tatggtcagg
     2941 gtggccgcgc agccaatcgg cattgcgcaa ggcataccag gaccagcagg cgacggctcc
     3001 caaagcgaat aacgccccgc tgacgtagcg cgcgaggtca acatgtgcgc tgttccgcag
     3061 cacgtcaagc tcgactcggt ttacgcagta aatcccgatg acaattaata gcaaggacgg
     3121 tgcgagacgc cgccaggcga tccgcccatc ccgtttggca ttgcgcagat tggccgatat
     3181 tgagatcacc actggcaatg tgccgatgat catggttggc aaagggccgc ccgcgcgctg
     3241 gatggcgctt gccaggcaaa ggtagtacag caaattgcca atggccgcca gtttcagcgc
     3301 ttctacccaa tccgcaacgg caaatcgatg cagcctcgtc cggtcgagcc acgccaaagg
     3361 gacggcaata atcccgaacg ccagataacg ccccaccgac tgcagtgcgg cggggtattc
     3421 gggcaaaagc agagggccta tgaaaaccag cccccagatc aagcctgccg tcagagcgaa
     3481 aaggattcca gaccacatga ttgatttcag ttgcggccgt ggattcccaa ttagttgtag
     3541 cgctgaaaaa tcagagtggc gtttgtcccg ccaaagccaa agctgtttga catgaccgta
     3601 ttcaacgtga cgttgtcccg gcgttcacga acgatgggta tgccctgcgc acccggatcg
     3661 agctggacaa tgttggctga agcgcttaga aagttttctt tcatcatcaa taatgaataa
     3721 atggcttcgt tcacccccgc tgcgcccagt gcatggccgg ttagggactt ggtcgaactg
     3781 accggcggca cggcgccgtc gccgaacacg gtgcgcaatg cttccagttc ccgtgtgtcg
     3841 cccacctgag tgctggtgcc atgtgcgttg atgtagtcga tttttcccgt taccgttgcc
     3901 atggcctgtt gcatgcagcg aacagcgccc tcgcccgagg gttgaaccat gtcgtaaccg
     3961 tccgaggttg cgccgtagcc agtaagctcg gcaatgatgt tcgcgccgcg cgcctttgca
     4021 tgttcaagct cttcaatcac cagaacgccg ccgccgcccg agatgacgaa gccgtcgcgg
     4081 gtcgcgtcat agggccttga agcggtggtg ggcgcgtcat tgtacttgga cgataaagca
     4141 cccatcgcgt caaacaagac agacatcgtc cagtgctctt cctcgcctcc gcctgcgaaa
     4201 acaatatctt gcttgcccat ctggatcagc tccattgcgt gaccgatgca atgcgcgctg
     4261 gtggcgcagg ccgagctgat cgagtaattg acgcccttga ttttgaaggg ggtcgccaga
     4321 caagccgtcg tggtgctgga catcgttctg ggcacgcgat aagggcctac gtgtttgacg
     4381 cctttttcgc gcaggagatc agccgcttcg acaatatttg aggtcgaagg gccacccgat
     4441 cccatcacca gaccggtcct gaaattcgaa atatcggatt catcaaggcc tgcgtcgtct
     4501 atcgcttgtt gcattgcaac atagctgaag gcggcgccgt cgcccatgaa tcgtttggtc
     4561 ctgcgatcaa tgagcgagtc cagatcaata ttcacaggtc catgaacgtg gctgcgaaaa
     4621 ccaagatctt tgtatgtttg cgaaaataca atccccgaac ggccctcttt gagggaatcg
     4681 atcacttctt ttttgttatt cccgatactt gaaacaatcc ccaggccggt aacaaccact
     4741 cttttcattg gattaccttt agaagtcttt tgtagacgta aataggccga cccgcaaatc
     4801 ggccgctttg taaataacga ggccgtccac ttccatgacg gcgtcggcaa tgcctaaaac
     4861 cagcttgcgc gagatgactc tcctcaaatt aatgcgatag gtcacttttt tgttggtggg
     4921 caacacctgc ccggaaaatt tcacctcgcc cacgcccaat gcccgccctt tgcccggatg
     4981 atcgagccag cataaataaa agcccaggag ttgccacatg gcatccagcc caaggcagcc
     5041 cggcatgacc gggtcgccct taaaatggca ggcgaaaaac cacaaatccg ggcgtatgtc
     5101 gagctctgcg atgacttcgc ccttgtcg
//
LOCUS       DATCXU010000453         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_127549,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000453 DATCXU010000000
VERSION     DATCXU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127549"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1902)
                     /gene="glmS"
                     /locus_tag="VIR04_10130"
     CDS             complement(64..1902)
                     /gene="glmS"
                     /locus_tag="VIR04_10130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY9461147.1"
                     /translation="MCGIVGAVAQRDVVPLLLEGLKRLEYRGYDSCGVAVYTDGELRR
                     ARSTQRVAELENQVKQERLSGFTGIAHTRWATHGAPATNNAHPHFSGQGRGNGGARIG
                     LVHNGIIENYDELRAELQGKGYVFESQTDTEVIAHLIDHLYEDDLFKAVQQACRRLTG
                     AYAIAVFCRDEPHRVVGARRGSPLVVGLGKNENFLASDALAIAGTTDQIIYLEDGDVV
                     DLQLARVWIVDADGREVKRQLHTVQLHTSAAELGPYRHFMQKEIFEQPRAVGDTLQNI
                     ESITPELFGDKAYKVFKAIDNVLILACGTSYYAGLTARYWIESIAKVPVNVEIASEYR
                     YRDSVPNPRTLVVTISQSGETADTLAALKHARDLGMEHTLTICNVASSAMVRECKLNY
                     ITRAGIEIGVASTKAFTTQLTALFLLALALAQVKGKITEEVEQDQLKALRHLPAAISA
                     VLALEPQIIAWAEIFATKENALFLGRGMHYPIALEGALKLKEISYIHAEAYPAGELKH
                     GPLALVTEHMPVVTIAPRDELLEKLKSNMQEVHTRGGELYVFADADTKMSSSEGVHVI
                     RMPENYGKLSPILHVVPLQLLAYHTAVTRGTDVDKPRNLAKSVTVE"
BASE COUNT          474 a          651 c          525 g          497 t
ORIGIN      
        1 ctttattacc tctggacaga ctttattttc ggtcttcaaa gggctaaaaa agccaaccgc
       61 ctcctactcc accgtcacac tcttcgccaa attcctaggc ttatccacat ccgtcccgcg
      121 cgtgaccgcc gtgtgatacg ccagcaactg cagtggcacc acgtgcaaaa tcggcgatag
      181 cttcccgtag ttttccggca tgcggatcac atgcacccct tcactgctcg acatcttggt
      241 atccgcatct gcgaacacat acagctcgcc gccgcgcgta tgcacctctt gcatattcga
      301 tttgagcttt tccagaagtt cgtcgcgcgg agcgatcgtc accaccggca tgtgctctgt
      361 caccagcgcc agcgggccat gcttcagttc acccgcgggg tatgcttccg catgaatgta
      421 gctaatttct tttaacttca gcgctccttc cagagcgatc gggtaatgca tgccgcggcc
      481 caggaataac gcattttcct tggtggcaaa gatttcggcc cacgcaatga tttgcggctc
      541 cagcgccagc accgcactga tggcggccgg caaatggcgc aacgccttaa gctgatcctg
      601 ctccacctct tccgttattt tgcctttcac ctgcgccagt gctagcgcca gcagaaacag
      661 tgccgtcaat tgcgttgtaa atgccttggt cgacgcaacg ccaatttcaa tcccggcgcg
      721 cgtaatgtag ttcagcttgc attcgcgcac catcgcgcta ctggccacat tgcatatcgt
      781 cagcgtatgt tccatcccca gatcgcgggc gtgtttaagc gcggccagcg tgtcggcggt
      841 ttcacccgac tgcgaaatcg tgacgaccag agtgcgtgga ttgggcacac tgtcgcgata
      901 gcgatactcg ctggcgattt cgacattgac cggcaccttg gcaatcgatt caatccagta
      961 acgggccgtc aagcccgcgt aatagcttgt gccgcacgcc agaatcaaca cattgtcgat
     1021 tgccttgaag accttgtacg ccttgtcgcc aaacagttcg ggagtgatgc tttcaatgtt
     1081 ctggagggta tcgccaacag cccgcggctg ctcaaatatt tctttttgca tgaaatgacg
     1141 gtaaggcccc agttccgccg cgctggtgtg caattgcacc gtatgcaact ggcgcttgac
     1201 ctcgcgcccg tcggcgtcga ctatccatac gcgcgccaac tgcaaatcga ccacatcgcc
     1261 atcttcaaga taaataatct gatcggtcgt gccggcaatc gccagtgcgt cggaagccag
     1321 aaaattttcg ttctttccca agcccaccac cagcggcgag ccacgccgcg cgcccaccac
     1381 acgatgaggt tcatcgcggc aaaatacggc aatggcgtac gcccccgtca gccgccggca
     1441 ggcctgttgc accgctttaa acaggtcgtc ttcatacaaa tgatcaatca agtgcgcgat
     1501 aacttcggta tccgtctggc tttcgaaaac ataacccttg ccttgcagct cggcgcgtag
     1561 ctcgtcgtag ttttcaatga tcccgttatg caccagaccg atacgcgcac cgccgttgcc
     1621 gcgcccctgg ccggaaaaat gcgggtgcgc attattcgtg gcgggcgcgc catgggtggc
     1681 ccagcgggtg tgtgcaatac cggtgaagcc cgagaggcgc tcttgtttga cctggttttc
     1741 aagttcggcc acgcgttgcg tgctgcgtgc acggcgcaac tcgccatccg tgtagacagc
     1801 cacgccgcaa gaatcgtaac ctcggtattc aaggcgtttt agcccttcca gaagcagggg
     1861 aacaacgtcg cgttgcgcga ccgcgccaac aatgccacac ataaatggac tccagttaat
     1921 ccgagaaacc cgcccattgt gcgcagctta gcaagaaatt tgatttcata tttattacaa
     1981 atatgaattt atattgcgtt aaggttatat tgtgaaataa tatttcacac aactgaaacc
     2041 acctttgtat aagtcatggc gccaaaatcc ataaaaaaga cccctgtttt tctggacgat
     2101 ctcgaccggc ggatcttgcg gcaactacaa agcgacagct cgcaaac
//
LOCUS       DATCXU010000454         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_757763,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000454 DATCXU010000000
VERSION     DATCXU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757763"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VIR04_10135"
     CDS             <1..271
                     /locus_tag="VIR04_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY9461148.1"
                     /translation="RRALAGMAFSGWVLCLGSLVHALYGLYPYAVNGTFTLSEISGKT
                     SPDVLLGGLIAYLVFYGIFLLGFFQLLKHIERYGVLPVARTRGRA"
     gene            268..1317
                     /locus_tag="VIR04_10140"
     CDS             268..1317
                     /locus_tag="VIR04_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY9461149.1"
                     /translation="MIGSLAAALGLGVLDPSFWMPLVFMGLLFTTIVAGTVLDGFDIG
                     VGCLVLLAPRKLRGRMLSLLSPWRDANEFWLFLGMGLFVAAFPHAWGNVMSELYLPLC
                     GLALGVLLRSVSFELRLRSPVEMQSRWLFGFALGSLITALAHGFLLGQLVISYQTQSG
                     YLWFSLFVAICALAAYCLLGAGWLIMRVGGELRARAVMWARHAVRWTAAGSVGVSLVL
                     AFANTGVFLKWSEGPQWPAVLFLWGCMLISFVSIEMCLQRMINHSYRTTALPFLMTLF
                     VFLATLGGLGYSFFPYLVLDDITIWDGAASVASLQLILSAAIIALPVALIFNIWVYWR
                     MFGLSRPPVPPAYKK"
     gene            complement(1247..>2184)
                     /locus_tag="VIR04_10145"
     CDS             complement(1247..>2184)
                     /locus_tag="VIR04_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9461150.1"
                     /translation="VAPPVAMPLILVWLGGMGLFLLLAAAFSWRITRPLTRLTKAADQ
                     LAAGQPQRVIPSGPTETRILGERFNIMLDTLAESSAVRRTLLAGLPHDLKGPLSRMWL
                     RIEMADDSTFKDGMRKDIQDMQRMVDHFIGFVRGTDPGTYRFAPLMLNVWLEEQVSAW
                     ESAGSDVLLTELWPQPLALDADRFALGRLLDNLIGNALNHGKPPVEIALTADSSVGII
                     TISDHGAGISADRRAEALRPFSRLDDARTLTGSVGLGLALADAIARAHQGSLTLGQAP
                     SGGLEVTVRLPLATSCKPAGPGAGTNQTCASKPKY"
BASE COUNT          358 a          594 c          672 g          560 t
ORIGIN      
        1 gcgtcgggcc ctggcgggca tggcgttttc tggctgggtt ctttgtttgg gcagtcttgt
       61 tcacgcgctg tacggcttat acccatacgc ggtaaacggc acattcacct taagcgaaat
      121 ctccggcaag acatcccccg acgtcttgtt gggcggcctg atcgcttatc tggtgtttta
      181 tggcattttc ctgctgggtt ttttccagtt gctcaagcat attgagcgct atggcgtttt
      241 gccggtagcc cggacacggg ggcgcgcatg ataggctcat tggcggccgc gctgggtctt
      301 ggtgtgttgg acccttcatt ttggatgccg ctggtcttca tgggcctgct ttttacgaca
      361 atcgtcgccg gcacggtgct ggatggcttt gatatcggag tagggtgtct ggttttgctt
      421 gcaccgcgta aattacgtgg gcgcatgttg tccttgctaa gtccctggag agacgccaac
      481 gaattttggc tatttttggg catgggtctg ttcgtcgccg cgtttccgca cgcctggggc
      541 aatgtgatga gcgaacttta tctgcccctg tgcggcctgg cgctgggggt attgctgcgt
      601 tcggtctcgt tcgagctgcg tttacgttcc cctgttgaga tgcagtcgcg ctggctcttc
      661 ggttttgccc tcggctcgct gatcacggcc ctggcgcacg ggtttttact ggggcaactg
      721 gtcataagct atcaaactca gtcaggctac ctttggtttt ctctgtttgt cgcaatttgc
      781 gcgctggcgg cgtattgcct gctaggcgcc ggttggctga ttatgcgggt aggcggcgag
      841 ttgcgggcgc gcgctgtgat gtgggcacgc cacgccgtgc gttggacggc ggcgggttcg
      901 gtgggcgttt cccttgtatt ggcgtttgcc aatacaggcg ttttccttaa atggagcgaa
      961 ggtcctcagt ggccggcagt gctttttttg tggggatgca tgctgatcag ctttgtatcc
     1021 attgaaatgt gcctgcagcg catgatcaat catagctatc gcaccaccgc cctgccgttt
     1081 ctgatgacgt tgtttgtctt cctggcgacg ctcggagggc ttggctatag cttttttccg
     1141 tatctggttc ttgatgacat taccatttgg gatggagcgg catccgtggc gtcgttgcaa
     1201 ctgattctct cggcggcgat cattgctttg ccagtagcgc tgattttcaa tatttgggtt
     1261 tactggcgca tgtttggttt gtcccggccc ccggtcccgc cggcttacaa gaagtagcaa
     1321 gcggcagtct cacggtcacc tccaggcctc ccgagggcgc ctggcccagc gttagcgaac
     1381 cctgatgcgc ccgcgcgatg gcgtcggcca gcgccagccc cagacccact gaaccggtca
     1441 gtgtgcgagc atcgtccagg cgggaaaatg gccgcaatgc ctcggcgcga cgatcggccg
     1501 aaataccggc gccgtgatcc gaaatggtaa taatgccgac gcttgaatct gccgtcagcg
     1561 cgatttccac aggtggcttg ccgtgattca aggcattgcc aatcaggttg tccagcaggc
     1621 ggcccagggc gaaacgatcg gcgtcaagcg ccagaggttg cggccacaat tcggtcaaaa
     1681 gcacgtcgct gccggcgctt tcccaggccg agacttgttc ttccagccag acattgagca
     1741 ttaatggtgc gaaacgatag gtgccggggt cggttccgcg tacgaacccg ataaagtgat
     1801 ccaccattcg ttgcatgtct tgaatgtctt ttcgcattcc gtctttaaat gtcgagtcat
     1861 cggccatttc gattcgcagc cacatgcgcg agagaggtcc tttcaaatcg tgcggcagtc
     1921 cggccagaag ggtgcgtcgc actgcgcttg attcggccag agtatcgagc atgatgttga
     1981 agcgttcgcc caagatcctg gtttctgtcg gccccgacgg gatgacgcgt tgaggttgtc
     2041 cggcggccag ttgatcggcg gctttggtca gccgggtcag cggacgcgtt atgcgccacg
     2101 aaaaagcggc ggcgagcaat agaaacagcc ccattcctcc cagccagacc aaaatcagcg
     2161 gcatggcgac cggcggggca accc
//
LOCUS       DATCXU010000455         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_635843,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000455 DATCXU010000000
VERSION     DATCXU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635843"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VIR04_10150"
     CDS             complement(<1..450)
                     /locus_tag="VIR04_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY9461151.1"
                     /translation="MPLTLVKARDQNSSDTELAQRVAHGDHGAIEALMRRYNQRLYRV
                     ARSILRNDADAEEAVQEAFYLAYRAMSKFRGDSQLSTWLVRIVVNESNKRLRKINRLS
                     TWLEFNEDPQDNDEAVEENMDRFSPDQPEQAMERAQTRHLIETRIDRL"
     gene            complement(545..2761)
                     /locus_tag="VIR04_10155"
     CDS             complement(545..2761)
                     /locus_tag="VIR04_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEY9461152.1"
                     /translation="MPPKSDLAHVTQLVALSFLTAKSARADFAVAEGDLQFQIEAHAP
                     GVYRLRCAEGKRLLDEKPSARVKAHAGMLLARQEPVGELAVSSMSEMQQKGWRLEQGE
                     ITLEITHSPLQLSLYRDKQCVLRTRAEDTLQRLTTDASCRWRFAIELDEDDALYGLGE
                     TAGELDRRGLHIVSDDPKQRALPLVWNPKGWGLYVNTIERVEHDLGAQQADLYTIDTG
                     GTIFDVFLFVGEPTEILNQFTALTGRPGQPGLWPLGVWLDQAPAQTISATLAQVEQFR
                     AGGFALDVVNLAEPALFGFQADKPVFEWDARRASDPRSMLAGFDALNLHLVAPSFPGV
                     LADTVLFSEWEDRGWLLLNDQDSSAYVFAGNAVTAGRPFGLLDLTHKDVYKLWSERHR
                     QLCDDGVGALACDAQFDIPDGVTARGGETGASLRTIYPLLARRALFDAVAGHKTPQEG
                     IVCSTDLFPSSQRYAWQTGLRTSNDWAGLERTLRTALSLGASAAVQTHGLGNASGPAQ
                     DMTSELYLRWLAMAVFSANFSFQGAPSLLPGAFDASTQALVRHWLGWRYRLIPYVLGI
                     IEDSVRTGLPVQRPMALAFPSDRLAHLWDTQYLLGPALLVAPVLQPGGTTQVYLPEGD
                     AWWDLNTGWRYEGGSTWTIEVGLDSLPVFGREGHMLCLGPTAQHTGEFNSARILDEVW
                     MFGMPEHSPVVMRNKIRVMQMQGSSYIKGLEGLRIVPCEGLEVKRRGAEVRISRAR"
BASE COUNT          694 a          894 c          779 g          583 t
ORIGIN      
        1 aagccggtct atcctggttt cgatcaaatg ccgcgtttgg gcgcgctcca tggcctgttc
       61 aggctggtct ggcgagaatc tgtccatgtt ttcctctact gcttcatcat tgtcttgcgg
      121 atcttcgttg aactcgagcc atgttgaaag gcgatttatc ttgcgcagcc gcttgttcga
      181 ttcattgacc acgatgcgca ccagccaggt cgaaagctgt gaatcgccgc gaaacttgct
      241 catggcccga tacgccaggt aaaacgcctc ctggacagcc tcttcggcat cggcatcgtt
      301 gcgcaagatg cttcgcgcca cccgataaag acgctggttg taccgtcgca tcaaggcctc
      361 gatcgcgccg tgatcgccgt gcgcgaccct ttgcgcaagt tcagtgtcag acgaattctg
      421 atcacgggct tttacgagtg ttaacggcat agcaaaacca gaaaaagact gcggttacag
      481 tattagagac cggtcgggcg atttgcgttc ccggtcaacc ccactggaac aggcgcttat
      541 tgtcttaacg cgcccgggaa atgcgcactt ctgcgccacg acgtttgacc tcaaggcctt
      601 cgcagggaac aatgcgcaag ccctcgagcc cttttatata gctcgatccc tgcatctgca
      661 taacgcgaat cttgttgcgc atgacgaccg gactgtgttc gggcatgccg aacatccaga
      721 cctcgtccaa tattcgcgcc gaattgaatt cgcccgtatg ctgcgccgtg ggtccaagac
      781 aaagcatgtg cccctcgcga ccgaacacag gtaaagagtc caggccgacc tcaatcgtcc
      841 aggtggagcc gccttcgtag cgccagccgg tattcagatc ccaccacgca tcgccttcag
      901 gcaagtacac ctgggtcgtt ccgcctggtt gcaaaacggg ggccaccaat aatgcggggc
      961 ccaataaata ttgggtatcc cacagatgag ccagtcggtc cgacggaaat gccagtgcca
     1021 tggggcgctg aaccggcaat ccggtgcgta ccgagtcttc aataataccg agcacgtaag
     1081 gaatcagacg ataacgccac cccagccaat gccgcaccag cgcctgcgtg gaagcatcga
     1141 atgctcctgg cagcaaagag ggcgcgccct gaaaactgaa attcgccgaa aacactgcca
     1201 tcgccaacca gcgaagataa agctcggaag tcatgtcttg cgctggcccc gatgcattgc
     1261 ccaagccatg cgtctggaca gcggcgcttg cgcccaaaga gagagctgtt cgcagcgtgc
     1321 gttcaagtcc cgcccaatcg ttggaagtcc gcaacccggt ctgccaggcg tagcgctgcg
     1381 atgagggaaa caaatcagta ctgcagacaa tgccttcttg cggcgtcttg tgccccgcca
     1441 cggcatcaaa caacgcacgt cgcgccagca aaggataaat ggtgcgcaag cttgccccgg
     1501 tctcgccgcc ccgagcggta acgccgtctg gaatgtcgaa ctgcgcgtcg cacgccagcg
     1561 cccccacacc atcgtcgcaa agctgccgat gccgctcgga ccataattta taaacgtctt
     1621 tatgcgtcag atccagcaga ccaaaaggtc ggccggccgt caccgcgttg ccggcaaaaa
     1681 cataggcgct gctgtcctgg tcgttcaata atagccagcc acgatcttcc cattcggaga
     1741 acaatacagt gtcggccaat acgcccggaa aggacggcgc gacaagatga agattaagcg
     1801 cgtcaaaacc cgccagcatg ctgcgtggat cggaagcgcg ccgcgcgtcc cactcgaaca
     1861 caggcttatc ggcctggaaa ccgaataatg cgggttcagc cagattaacg acatcaagag
     1921 caaaaccgcc cgcccggaat tgttcgacct gcgccagcgt cgcactgatc gtttgagcgg
     1981 gcgcctggtc aagccacacc ccaagcggcc ataaacctgg ctgtccgggg cgccctgtaa
     2041 gcgccgtgaa ctgattcagg atttcggtgg gttcgcccac aaagagaaat acatcaaaaa
     2101 tcgtgccgcc cgtatcaatc gtgtagaggt cggcctgctg cgcacccaaa tcatgttcaa
     2161 cccgctcgat ggtgttgaca tacaggcccc accctttggg attccacacc agcggcagtg
     2221 cgcgctgttt ggggtcgtcg gacacaatgt gcagaccacg gcgatccagc tcgccagcgg
     2281 tctcgcccaa accgtacaag gcgtcatcct catcgagttc aatcgcaaaa cgccaccggc
     2341 atgatgcgtc ggtcgtcagg cgctgcaagg tatcttcggc ccgagtgcgc aagacacact
     2401 gtttatccct gtacaaggaa agctgcaacg gcgaatgcgt tatttcaaga gtgatttcgc
     2461 cctgctccag gcgccaccct ttttgttgca tttcggacat cgaactgacg gccagttcac
     2521 ccaccggctc ttgccgggcc agaagcatgc ccgcatgcgc cttgactcgg gcgctgggct
     2581 tttcatcaag caaacgcttg ccttcggcgc aacgcaggcg ataaacgcca ggagcatgag
     2641 cttcaatttg aaactgcaga tcgccctcgg caacggcaaa atccgcacgc gcggattttg
     2701 ccgtcaggaa actaagggca acaagttgcg tcacgtgagc aaggtcagac ttgggcggca
     2761 cagcgcaatc ctcgggcata gcccaaaaag cattcaaaag gcattattct gactgattta
     2821 ggccataaaa taaaatcgaa tcatttcatg aacgtctaaa aaccgtcaaa atatcgttaa
     2881 cgcggggcta ttgcccgtaa caattctcgt tcaatcggcg caggctcaag gttcaattgc
     2941 gcggcaataa
//
LOCUS       DATCXU010000456         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_599121,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000456 DATCXU010000000
VERSION     DATCXU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599121"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VIR04_10160"
     CDS             complement(<1..435)
                     /locus_tag="VIR04_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461153.1"
                     /translation="MNKKIFWTIVLWIALLTMPYWMGYVGGYTGLATRVLVLGLAAMS
                     LNFLLGFTGVLSFGHAAYFGLGAYGAGLLLKYQYFPPSTVLAIFEGVLVATVAAAVVG
                     ALIVKLRGIYFAMVTIAFGQMFYFIAFRWSSVTGGDDGLTGWQ"
     gene            complement(441..1310)
                     /locus_tag="VIR04_10165"
     CDS             complement(441..1310)
                     /locus_tag="VIR04_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461154.1"
                     /translation="MTELLLFNVFNGLIVGAFYALMALGLSLIINLTSVINFAHGGFL
                     ALGAYIAYELVPYFGFWGALIVAPVLTALLGLAVERVLIRRLYGRDPLYSLLLTFGLA
                     YVFQDGVRYIWGAQSLPFRVPAWLTQPLSNYFFFLTGYRVFMVLLVAVAVAGLFYILN
                     HTRLGIRIRAGTLDLETVSVLGVNVRILRNLNFGLGIYLAGLAGVLAAGLLGLQPTIG
                     DSLIMPSFVAIIVGGIGSLFGTLLGGLLIGTASGIATVFWPAASEAIIYIMMALVLLV
                     RPRGLLGEEGMTL"
     gene            complement(1441..>2497)
                     /locus_tag="VIR04_10170"
     CDS             complement(1441..>2497)
                     /locus_tag="VIR04_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461155.1"
                     /translation="EQINAKGGILGRPAELLIEDSSSGDAGIAVQKARKLIDRDKVSF
                     LLGNVNSGLSVAMAQVSYEKKVLHIVPGGHTDTVTGATCHWNVFRVCNTTRMLTNAIA
                     KSLMDRYGKKMYFITSDYAFGHTLQDGYDANLKQYGGTKLGEDLTPLGTTDFSSYLIK
                     AQATKPDVIVFLMNGDDLLNALKQAVQFGLDKRTHLAGANTELEVLEGLPPEARIGDW
                     VAEWYHIQPGVPHVDEFVAAIKKKTGRVPTARTWFGFVGTWSCALAANKAKSLDAFKM
                     AKALDGMQLPPEVGLMPNLPFYRSNQHQLIGSLYVGNAQAKGSAPDDLFHVAKVIDGK
                     EVAGTVEESGCKMTWPA"
BASE COUNT          626 a          723 c          680 g          468 t
ORIGIN      
        1 ctgccagcct gtcagcccgt cgtcgccgcc cgtgaccgag ctccagcgaa acgcgatgaa
       61 gtagaacatt tgcccaaagg caatggtcac catcgcgaag tagatgccgc gcaatttgac
      121 gataagcgcg ccgaccacgg ctgccgccac tgtcgcaaca agcaccccct cgaaaatcgc
      181 gagcacggtg ctgggaggga aatactggta cttcagcaac agcccggcgc cgtatgcccc
      241 aaggccgaaa tacgccgcat gcccgaagga cagcacgccg gtgaagccca gcaaaaagtt
      301 aagcgacatg gccgccagac ccagcaccag cacacgcgtg gcaaggcccg tgtaaccgcc
      361 cacatagccc atccagtagg gcatggtcaa taatgcaatc cagaggacta tcgtccaaaa
      421 tatttttttg ttcatgccgt tcacagggtc attccttctt caccaagcag gccgcgcgga
      481 cgaaccagca agaccagcgc catcatgatg tatatgatcg cctcggaggc cgccggccag
      541 aataccgtgg caattccgga cgcggtgcca atcagcaaac cgcccagcaa tgtgccgaac
      601 agactgccaa taccgccgac gataatggcc acgaagctcg gcatgatgag cgagtcgcca
      661 attgtcggtt gcagcccaag caaaccggcc gccagcacgc ctgccagacc agcgagataa
      721 atgcccaggc caaagttgag attgcgcaat atgcgaacat taacgcccag cactgaaacc
      781 gtttcaagat ccagggtgcc cgcgcgaata cggatgccga gccgggtgtg attcaaaata
      841 taaaagagcc ccgcaaccgc caccgccacc agcaaaacca tgaatacgcg atagccggtc
      901 aggaagaaga agtaattgct taagggctgg gtcagccacg cgggcacgcg aaacggcagg
      961 ctttgcgccc cccaaatgta tcgcacgccg tcctgaaaga cataagccag cccgaaggtc
     1021 agcaacaagc tgtagagcgg atcgcggccg tataaacgac ggatcaacac gcgttcgacg
     1081 gccagcccca gcaaagccgt gagcacaggc gcaacgatca gggcgcccca gaaaccgaaa
     1141 tagggaacca gttcataagc gatgtaggcg cccaaggcca gaaacccgcc atgggcgaaa
     1201 ttgataacgc tggtcaaatt gatgatcagc gacaggccca gcgccatcag cgcgtaaaac
     1261 gcaccgacaa tgaggccgtt aaaaacgtta aataatagta gttcggtcat tcagtaattt
     1321 gccttgtgac gcacccaccg tatacaaaca gccgccttgt caaactcagg cggcgattcg
     1381 cagagcggag cggaacgtta cgggcgccgt aataatatcg gcgcccgggt tatcgaacta
     1441 tcaagcgggc caggtcattt tgcaaccgga ctcttcgacg gtgcctgcaa cttctttgcc
     1501 gtcgatcact ttggcgacat ggaacaggtc atcgggtgct gaccctttag cctgggcatt
     1561 gccgacatac agcgagccga tcaattgatg ttgattggag cggtagaacg gaaggttagg
     1621 cataaggccg acttcgggcg gcaattgcat gccgtccagc gctttggcca ttttgaaggc
     1681 gtcgagcgac ttggctttgt tggcagccag cgcgcagctc caggttccca cgaagccgaa
     1741 ccacgtacgc gccgttggaa cacggccagt ctttttcttg atggcggcga caaattcgtc
     1801 gacatgagga acaccaggct gaatgtgata ccactcggca acccagtccc cgatgcgggc
     1861 ttcgggcggc aagccttcga gcacttcgag ttcggtgttg gcgccggcca gatgagtccg
     1921 tttgtcaagg ccgaattgaa ccgcctgctt gagagcgtta agcagatcgt cgccattcat
     1981 caagaaaaca ataacatccg gttttgtcgc ttgcgccttg atcaggtacg acgagaaatc
     2041 ggtcgtgccc aatggggtca agtcttcgcc cagcttggtg ccgccgtatt gcttcaggtt
     2101 cgcatcgtag ccgtcttgca aagtatggcc gaaagcatag tcggaagtga tgaaatacat
     2161 cttcttgccg tatctgtcca ttagcgactt ggcaattgca ttggtcagca tgcgcgtggt
     2221 gttacatacc cggaatacgt tccaatggca ggtcgcgccg gtgacggtat cagtatgccc
     2281 gccgggaaca atatgcagaa ctttcttttc gtagctaacc tgcgccatag ccacggaaag
     2341 accagagttc acattaccca gaaggaagct gactttgtcg cgatcaatga gcttgcgtgc
     2401 tttttgcact gcaatgcccg cgtcgccgct gcttgaatct tcgatcagaa gttcggccgg
     2461 acggccaaga atgccgcctt tggcattgat ctgttca
//
LOCUS       DATCXU010000457         9033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_46396,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000457 DATCXU010000000
VERSION     DATCXU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46396"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIR04_10175"
     CDS             complement(<1..340)
                     /locus_tag="VIR04_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY9461156.1"
                     /translation="MEPPLKNQKPPFQQTSISVEGQHVSALTLGTGPAIVLLHSLLAD
                     YSSFNLIAQPLSRTHKVVLLNLPGFGSSDSVNGGLDAVADRVAGAINALEFEQAPILL
                     GNGYGGFIATT"
     gene            complement(381..1916)
                     /locus_tag="VIR04_10180"
     CDS             complement(381..1916)
                     /locus_tag="VIR04_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY9461157.1"
                     /translation="MSTINAQTSMPGVPLHGLFIDGHEVAATRPDLLDVINPATGELL
                     AQIGHASDADVDRAVRSADTAFHSQEWSAMSNRTRAKLVNKLADVFEDNLEEIYHLET
                     TNNGRPLSETRAQVSRLPDFLRYNAGLAIARRDAVIPVDGNYLNYTIRTPVGVVGNST
                     PFNHPLMILIKGLAPTLASGCTTVVKPSEYTPLTTLRLAQLFVEAGLPPGVFNVVTGL
                     GPTTGKALAAHPGIAKWVLTGGTDAGRITGALAGGNFAHQTLELGGKTPVLVFDDFDV
                     DQAVNYAAFGGFIGAGQTCICGSRLIVQEKIYDEFVEKLAIKAKSIRIGDPADATVQL
                     GPVVSARQQQRILKYIKIGLEEDRARLVAGGHIPSDPSLKNGFFIEPTVFADVTPRMR
                     IFQEEVFGPFVSISKFSTEDEGLAMANDSPFGLAGAIRTNDITRAHRVAGLLKCGIVW
                     INDHHRLDPASPWGGVKDSGSGRECGTESFDQHFETKSVMVRLSDAPFDWYKDSASQS
                     RLN"
     gene            complement(1941..2621)
                     /locus_tag="VIR04_10185"
     CDS             complement(1941..2621)
                     /locus_tag="VIR04_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY9461158.1"
                     /translation="MKSSEAPSPITGNQLLRESIYEELRADILRCKLPPGTEIEAAEV
                     AAHFRVSKSPVRDALMHLEREGLVITVPRQGYRVAPISLTDVQDMFHLRACMERACMQ
                     RIVQRASDAELAELNKFRVFDAKTWPDGFVAYNRAFHRSLAELAKNARMRDQLGDLID
                     QMERAVLMSLSNVKKGNPNSILKEHCQIIDALQSRQLKLAERLAERHISAAAKRVDDA
                     ISRIVVAN"
     gene            2820..4076
                     /locus_tag="VIR04_10190"
     CDS             2820..4076
                     /locus_tag="VIR04_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461159.1"
                     /translation="MLKKLLTFTALTFALAGSLPMQTATAQSANKDPIRLGFLTIQSG
                     ALAAGGRQMEEGLRTFLKERNNMIAGRRVELFIGDTAGQPAVTKSKAQELVEKDKVQV
                     IIGPLAAFEALAIDDYIRKVKVPIISPSAAAEDLTQRKLNPWFVRAVGTSAQPTQPLG
                     EYAAKDLKYKRVVTIGDDFAFGYESVAGFQRTFEENGGKVVQKLWAPLNVADYGTYIS
                     EIDPTADAICVNFAGSNSVRFLKQYKAYGMKGKMPVIAGMIAMDEGILKSMGDEALGI
                     VSAGWYAATIDNPANKRFVQEMNKTYKEDPGYYSIGAYGSALMLEKALTAVNGNIEDK
                     DAFMKALRSVSLSNDPRGDFKLDAYGNPIMNVYIRKVERQGGKLVNTIVKTYPAVSQF
                     WTYDPKAFLANPVYSRDYPPAKNLKK"
     gene            4113..4973
                     /locus_tag="VIR04_10195"
     CDS             4113..4973
                     /locus_tag="VIR04_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461160.1"
                     /translation="MSFWLIQSLNSLALGGILFTLAAGFSLIFGLMRIANLSHGAYFM
                     LGAYIGLSTLKYSENFLAAMLVASVSIGVLGVAIERLVLRRLAGDSLSQVLATLGIAF
                     IIADFSLWFWGGDPQTVSPPDLLSGAMHMFGFGFPMYRVAVLLFSVVLALVLWVLLEK
                     TRLGMMIRASVDDRQMARGVGIPSSRLFSIVFCLGAALAGVGGVVASPILSIYPGMDM
                     DMLPLALVVVILGGVDSLAGAFVGSFLIGFIYSFGQVLFPNLAYVILFLPMVIILAIK
                     PRGLFGRTTA"
     gene            4970..5971
                     /locus_tag="VIR04_10200"
     CDS             4970..5971
                     /locus_tag="VIR04_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461161.1"
                     /translation="MKRVLYAALLVLLLCLPWWAGGEYYINLASQILIAALFAVSLNL
                     KVGYAGLISLGHAGYLGLSAYLSSWLMLNLQWGHFPAAMGALAGTTIMAALFGLVALR
                     ATGLGFLMITLALGQILWGVAFRWADVTGGDNGLSGTTRPSPFGVDLSGADNFYYFTL
                     GIFLLVWCLIAIWVRSPFGASIRGSKDQPRRMSALGYNVWLIRWITFVVSGFFAAIAG
                     LMYVYYQQFISPHSLSLTNSAEMLLMVIAGGPGTLLGPVVGATLVVLLKNVASAYINR
                     WIMLLGFSFLFIVLFVPGGIVPGVMRAVDSWRQRQSRRAASKIVSSPSDVPAVKETP"
     gene            5968..6708
                     /locus_tag="VIR04_10205"
     CDS             5968..6708
                     /locus_tag="VIR04_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461162.1"
                     /translation="MTLALSVSSLCKSYGGLKVTRAVDLDVEQGERHLLIGPNGAGKT
                     TLFNLIAGDLAPDSGEIRLMGRNVTHTSTASRAQAGLARTYQIVTLFPRDSLLHNVML
                     ALMGKSALRWRHWGAFAGQPEMTQSALEALELVGLSDKACLPVDQTSYGEKRRVEIAM
                     ALSQRPKVLLLDEPFAGLSSEERKTIYALLCSIDREITVVMIEHDMDVALAFADRITL
                     LHYGEVITVGTRQQVVDDPRTRELYLGK"
     gene            6698..7414
                     /locus_tag="VIR04_10210"
     CDS             6698..7414
                     /locus_tag="VIR04_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461163.1"
                     /translation="MENKLLELDRVNAFYADSHVLHDVSLSLKEGRLLALLGRNGAGK
                     STCISTVVGFLPPRSGSVRLDGRDIQASSPESICRSGVGLVPQGRRVFGSLTVRENLD
                     VAATGRRPAGRRYWTRDEINSIFPRLRERQNQLAGSLSGGEQQMLAIGRALMTNPKVL
                     LMDEPSEGLAPQIVHEVGRVLETLKSEISIVLVEQNLGLALNVADDVVLLSTGRVVFA
                     GTREAFQAQQSELHAHLGIT"
     gene            complement(7447..8277)
                     /gene="galU"
                     /locus_tag="VIR04_10215"
     CDS             complement(7447..8277)
                     /gene="galU"
                     /locus_tag="VIR04_10215"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY9461164.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKAMPKEMLPIVDKPLIQYAVEE
                     AIAAGITDLIFITGRNKRAIEDHFDSAPELEAELESKHKSKLLDVVRNVIPSYVNCIY
                     IRQPAPLGLGHAVLCAQPIVGDEPFAVLLADDLIDADVSATQQLVDAARAHNGSVLAI
                     QNVPRNQTGRYGIIAGPAIDAQTTRVERIVEKPDPADAPSTLAVVGRYVLEPDIFDHL
                     RKTQSGVGGEIQLTDGIASLLKTRPVYGFQYKGLRYDCGSKAGFFQATMELGRKYHGL
                     "
     gene            complement(8502..>9033)
                     /locus_tag="VIR04_10220"
     CDS             complement(8502..>9033)
                     /locus_tag="VIR04_10220"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY9461165.1"
                     /translation="VRLQTSQPPLMLASFGTATTIDTLAIDTAVQSPSTDTPQSNNWL
                     FLGGLIFPGATLMRSSLVEGTANLPEASGTAAAYPTNTHQAIVTGIAAAQAGAVLRQW
                     LTGLEVSGQAPRVYSSGGAWPIVKDETQRLLTAAQKRMGLHETPIEWLPSPVLDGLAV
                     LALGQGSLRASQFALH"
BASE COUNT         1744 a         2311 c         2776 g         2202 t
ORIGIN      
        1 cggtggttgc tatgaacccg ccatagccgt ttccaagcag aattggcgcc tgttcgaatt
       61 caagcgcgtt gatggcgccg gcgacccggt cagcgactgc gtcgaggccg ccattgacgc
      121 tgtcggagga gccgaacccg ggcaggttta acaaaaccac cttgtgagtc ctggagagcg
      181 gctgcgcgat caggttaaag ctggaatagt cggccagaag cgaatgaagc aggacaatgg
      241 cggggcccgt gcccaaggtc agtgcactga catgctgacc ttcgacagaa atgcttgtct
      301 gttgaaatgg aggtttctga ttcttgagtg gtggttccat tattcgcctt tgttgtaggg
      361 gcatctggtc gttttgacgc ttaattcagg cgcgattggc tggccgagtc tttgtaccag
      421 tcgaatggag catcgcttag ccgcaccatg acgcttttgg tttcgaaatg ctggtcgaaa
      481 gattcggtgc cgcactcgcg gccggatccg gaatctttca ctccgcccca gggcgatgcg
      541 ggatcgaggc ggtggtggtc gttgatccat acgatgccgc acttgagcaa gcccgctacg
      601 cgatgagccc gtgtgatgtc attggtgcgg atggcgccgg ccaggccgaa gggcgaatcg
      661 ttggccatgg ccagcccttc gtcttcggtc gaaaacttgc ttatcgagac gaatgggcca
      721 aagacctctt cctggaaaat gcgcattctg ggggtgacgt ccgcaaaaac ggtcggttcg
      781 atgaagaagc catttttcaa tgacgggtcc gaaggtatgt gtccgcccgc aaccaggcgt
      841 gcgcgatctt cttccagtcc gattttgatg tatttgagta ttcgttgttg ctggcgggcc
      901 gaaacgaccg ggccaagctg gactgtggcg tcggccggat cgccaatgcg gattgacttg
      961 gcctttatgg ctaatttttc aacgaattcg tcgtatattt tttcctggac gatcagccgg
     1021 ctgccgcaga tgcaggtttg tccggcgcca atgaacccgc cgaaggctgc atagttgacc
     1081 gcttgatcaa catcgaaatc atcgaacacc agtacgggcg ttttaccgcc cagctcgagt
     1141 gtctggtggg cgaaattgcc gcccgccagc gcgcctgtta tgcggcccgc atccgtgccg
     1201 cctgtaagaa cccatttcgc gatccccggg tgggcggcaa gggccttgcc cgtggtgggg
     1261 cccaggccgg ttacgacatt gaatacgcct ggaggcaatc cggcttcgac gaacagttgg
     1321 gccagacgca atgttgtaag cggcgtgtat tcggagggtt tgaccactgt cgtgcatccc
     1381 gaagccaggg tgggagccag gcctttgatg agaatcatga gcggatgatt gaatggcgtt
     1441 gagttgccca ctacgccgac cggggtgcgg atcgtgtaat tcaagtagtt gccgtcaacg
     1501 gggattacag cgtcgcgccg ggcaattgca agcccggcgt tgtagcggag aaaatcgggc
     1561 agtctggata cctgagcgcg ggtttcacta aggggccggc cgttgttggt ggtttccagg
     1621 tgatatatct cctcgagatt gtcttcgaac acgtcggcca gtttattgac gagcttggcc
     1681 cgggtccggt tggacatggc gctccattcc tggctgtgga aggcggtgtc ggcgctacgg
     1741 acggcgcggt cgacatcggc gtcgctggcg tgaccgatct gcgccagcag ttcgcctgtt
     1801 gccgggttga tgacatccag cagatcgggg cgggtcgctg ctacttcgtg cccgtcgatg
     1861 aacagcccat gcaggggaac cccgggcatg ctcgtttgtg cgttgatagt cgacatgttt
     1921 ggccttcgta tggttagaaa ttaattggcg acgacaatcc tggagattgc gtcgtccact
     1981 cgcttcgccg ccgcggaaat gtggcgctcc gccaggcgtt ccgccagttt taattggcgc
     2041 gattgcagcg cgtcgataat ctggcaatgt tccttgagga tcgaattggg gtttcccttt
     2101 tttacgttgc ttaaactcat cagcacggcg cgttccattt ggtcgatcaa gtcgcccagt
     2161 tgatcgcgca tgcgtgcatt ttttgccagt tcggccagcg accggtgaaa ggccctgtta
     2221 taggcgacga acccatcggg ccatgttttg gcgtcgaaaa cccggaattt attgagttcg
     2281 gccagttcgg catcgctggc acgttgcaca attcgttgca tgcaggcgcg ttccatgcag
     2341 gcgcgcagat gaaacatgtc ttgcacatcg gtcaacgata tgggggcgac ccgataaccc
     2401 tgtctgggta cggttattac caagccttca cgttcaaggt gcattaatgc atcgcgaacg
     2461 ggcgatttgc tgacccgaaa gtgggcggcg acttcggccg cttcgatttc cgttcccgga
     2521 ggcagcttgc atcgcagaat atcagcccgc agctcttcgt atatggattc gcgcagtagc
     2581 tgatttccgg tgatcgggga gggtgcttcg cttgatttca ttggctctat aaaatttcac
     2641 tgagtgtcta aattgaatta tgtaatatat ctcgtgatat tgcaataatg cctcgtgcac
     2701 tagggtaaac cttgatcaca cagtttattt tgttattttt tttgtcaatt ttattgactt
     2761 aaatttaatt cgtaatatat cattggcgaa aaataaatcg aattttacgg agacaagcta
     2821 tgctcaaaaa gcttttgact ttcactgcat tgacttttgc gcttgcgggc tcgttgccca
     2881 tgcaaaccgc aacggcgcag tccgccaaca aagatccaat ccgtctggga ttcctgacga
     2941 ttcagtcggg cgccctggcg gcgggcggca ggcagatgga agagggcttg cgcaccttcc
     3001 tgaaggaacg caacaacatg attgcgggcc gccgggttga attgtttatt ggcgacacgg
     3061 cggggcaacc ggccgtcacc aaaagcaagg cgcaggaact ggtggaaaaa gacaaggtgc
     3121 aggtaattat cgggccgctg gctgctttcg aggccctggc catcgatgat tacattcgca
     3181 aagtcaaggt gcccatcatt tcgccgtccg ccgccgccga agacctgacg cagcgtaaat
     3241 tgaatccgtg gttcgtgcgc gcggtcggca catcggcgca acccacgcaa ccgcttggcg
     3301 agtacgcggc aaaagatttg aagtacaagc gggtcgtgac cattggcgac gactttgcct
     3361 ttggctacga aagcgtagcg ggctttcagc gcacattcga ggaaaatggc ggcaaggtgg
     3421 tgcaaaagct gtgggcgccg ttaaatgttg ccgactacgg cacctacatc agcgagatcg
     3481 acccgactgc ggatgccatt tgcgtcaact tcgccggcag caatagtgtt cggtttctca
     3541 agcaatacaa agcgtacggc atgaaaggaa aaatgccggt gattgcagga atgattgcca
     3601 tggacgaagg cattctaaaa agcatgggcg atgaggcgct gggcatcgtt tccgcgggct
     3661 ggtatgcggc cacgatcgac aaccctgcca acaagcgctt tgttcaggaa atgaacaaga
     3721 catacaaaga agaccctggc tattattcga taggcgctta cgggtcggca ttaatgctgg
     3781 aaaaggccct gacagccgta aatggcaaca tcgaggacaa agacgctttc atgaaagcat
     3841 tgcgcagcgt ttcgttgtcc aatgacccgc gcggcgactt caaacttgat gcgtatggca
     3901 atccgatcat gaacgtctac atccgcaagg tcgaacgtca aggtggaaaa ctggtcaaca
     3961 cgattgtcaa gacttatccg gcggtcagtc agttctggac ttacgacccg aaggcattcc
     4021 tggccaatcc ggtgtattcg cgcgactatc cgccggccaa aaatcttaag aaataactac
     4081 attcaacatt tgcacggagc ctgattcaga ttatgtcgtt ttggcttatc caaagtttga
     4141 atagcctggc gctgggtggc attttgttca ccctggcggc cgggttttct ttaatttttg
     4201 ggctgatgcg catcgccaat ttgtctcatg gggcgtattt catgcttggc gcctatattg
     4261 gcttgagcac gctcaagtac agcgaaaact ttctggccgc catgctggtc gccagcgtct
     4321 cgatcggcgt tttgggtgtc gcaatcgagc gcctggtgct tcggcggctg gcgggagaca
     4381 gtctgtcgca ggtgctggcg acacttggaa ttgcattcat tattgcggat ttcagccttt
     4441 ggttttgggg cggtgatccg caaacggttt cgccgcccga cctgctttcg ggcgcgatgc
     4501 acatgttcgg tttcgggttt ccaatgtatc gcgttgccgt gcttttgttc agtgttgttc
     4561 ttgccctcgt tttgtgggtt ctgcttgaaa agacccgcct cgggatgatg atccgggcaa
     4621 gtgttgatga cagacaaatg gcgcgtggcg tcggcatccc ttcttctcgt ctgttcagca
     4681 tcgtgttttg tctgggcgcc gctctggcgg gcgttggcgg cgtggtggcc tcgcccattt
     4741 tgtcgatcta tccgggcatg gatatggaca tgttgccgct ggcgctggtg gtggttattc
     4801 tgggcggggt ggacagcctg gctggagcgt ttgtcggcag cttcctgatc gggttcattt
     4861 atagctttgg gcaggtgctt tttcccaacc tggcgtacgt catcctcttt ttgccgatgg
     4921 tgatcattct tgcaatcaag cctcgcggcc tgtttgggcg aacaaccgca tgaaacgcgt
     4981 attgtatgcg gcgttgctcg tgctgctgtt gtgtctgcca tggtgggcgg ggggcgagta
     5041 ttacatcaac ctcgccagtc aaattcttat tgccgctcta tttgccgtca gtctgaacct
     5101 taaggtgggt tacgccggat tgatctcgct cgggcatgcg ggctacctgg ggttatccgc
     5161 gtaccttagc agttggctca tgcttaattt gcaatggggt cattttccgg ctgcgatggg
     5221 ggcgctggcc gggacaacca ttatggcggc gctgtttggt ctggtcgcgt tgcgtgcgac
     5281 gggattaggt tttttgatga ttacgctggc gctggggcaa atactttggg gggttgcgtt
     5341 ccgttgggcc gacgtgacag ggggcgataa cggcttgtcg ggcaccaccc ggcccagccc
     5401 ctttggcgtg gatttatcgg gcgccgataa cttctattat tttacgcttg gcatatttct
     5461 gcttgtctgg tgtcttatcg ccatctgggt tcgctccccc ttcggcgcca gcatacgggg
     5521 ttccaaggac cagccccgcc gcatgagcgc attgggctat aacgtttggc tgatacgctg
     5581 gatcaccttt gttgtgagcg ggttctttgc cgcgattgcc ggtctgatgt atgtgtatta
     5641 ccagcaattc ataagtccgc atagcctgtc gctgacgaac tcggccgaga tgcttcttat
     5701 ggtgattgcc ggcggtccgg gcacgcttct ggggccggtt gtgggcgcta ccctggttgt
     5761 tctgttgaaa aacgtggcaa gcgcttatat caaccgatgg attatgctgc tggggttctc
     5821 ttttttattt attgtgctgt tcgtgcccgg cggaattgtt cccggggtga tgcgcgcagt
     5881 cgactcctgg aggcagcggc aatctcgaag ggcggcgtcg aaaatcgtgt cgtcgccatc
     5941 ggatgtgccc gcggttaagg aaacaccatg acattggctc tgagcgtttc cagcctgtgc
     6001 aaatcgtacg gcggtttgaa agtgacgcgc gcggttgatc ttgatgtcga acagggcgag
     6061 cgccatcttt tgatcggtcc gaatggcgcg ggcaaaacaa cgttgttcaa tttaatcgcc
     6121 ggcgacctgg cgccggattc gggtgaaatc cggctgatgg ggcgcaatgt aacccatacg
     6181 tcgacggcct ccagggcgca ggccgggctt gcccgcactt atcagatcgt tacgctgttt
     6241 ccgcgggaca gcctgttgca taacgtcatg ctggctttaa tgggaaagtc ggccctgcgc
     6301 tggcggcatt ggggagcgtt tgccgggcag cctgagatga ctcaaagcgc gcttgaggcc
     6361 cttgagctgg tgggtttgtc ggacaaggcc tgtttgccag ttgatcagac atcttacggc
     6421 gaaaaaagaa gggttgagat agccatggcg ttatctcaac gacccaaagt tctgctcctt
     6481 gatgaacctt tcgccgggtt gtcgtccgag gaacgcaaaa caatttacgc gttgttgtgt
     6541 tccatcgacc gggaaatcac tgtagtcatg atcgagcacg acatggatgt tgcactggct
     6601 tttgcggatc gcatcacact gctgcactat ggtgaagtca tcaccgtggg cacgcgtcag
     6661 caagtcgtcg atgatccgcg cacccgggag ctttatcttg gaaaataagc tgttggagct
     6721 tgaccgggtc aatgcgtttt acgcggatag ccacgtattg cacgacgtgt cgttatcgtt
     6781 gaaggaaggc cgtctgctgg ctttgctggg tcgaaacggg gccggaaaat ccacatgcat
     6841 cagcacggtt gtggggtttc tgccgccgcg atcagggtct gttcgactcg atggccggga
     6901 cattcaggct tcttctcccg agtcgatctg ccgctctggc gttggattgg ttccgcaagg
     6961 gcggcgtgtg ttcggcagcc tgaccgtgcg ggaaaatctg gacgttgccg caaccgggcg
     7021 gcgccccgcc gggcgtcgtt actggacccg ggacgaaatc aattcgattt ttccgcgcct
     7081 gcgtgaacgc cagaatcaat tggccggcag tttgtccggc ggcgagcaac agatgcttgc
     7141 catcggaaga gcgcttatga ctaatcccaa ggtgttgttg atggatgagc catccgaggg
     7201 cctggcgccg caaatcgtgc acgaagtggg gcgggtcctg gaaacgctga aatccgagat
     7261 atcgattgtt ctggtcgagc agaatctggg gttggcgtta aacgtcgccg acgatgtggt
     7321 tttgcttagc accggccggg tggtatttgc gggtactcgc gaggcatttc aggcgcagca
     7381 gtcggaactg catgcgcatc tgggtattac gtagccgctt tcggatggcc cgcggcggct
     7441 tgccgcttac aagccgtggt acttgcgccc cagttccatt gtggcctgaa aaaaacccgc
     7501 cttgctgccg caatcgtagc gcagaccctt gtattggaag ccatagaccg ggcgcgtttt
     7561 cagcaggctg gcgatgccat cggtaagctg gatttcgccc cccacgccgc tctgggtttt
     7621 tcgcaggtgg tcgaaaatat cgggttccag cacatagcgg cccaccacag ccagggtcga
     7681 gggcgcgtcg gccgggtcgg gcttttcgac aatacgttcc acccgcgtag tttgcgcatc
     7741 tatcgcgggg cccgcgatta tgccgtatcg gccggtttgg ttgcgcggga cgttttgaat
     7801 ggccagcacg ctgccgttgt gcgcgcgcgc ggcatcgacc aattgttgcg tggcggatac
     7861 gtcggcgtcg atcaaatcgt cggccaacag cactgcgaac ggttcgtcgc cgacgatggg
     7921 ctgggcgcat aaaaccgcgt gccccaaacc cagcggcgcg ggttgccgga tatagatgca
     7981 atttacataa gaagggatga cgtttcggac gacatcgagc aacttgcttt tgtgtttgga
     8041 ttcaagttct gcttcgagtt cgggcgcact gtcgaaatga tcttcgatgg cgcgtttgtt
     8101 gcgcccggta atgaaaatca aatctgtgat tcccgccgcg atggcttctt cgacagcgta
     8161 ttggatgagc ggtttgtcga cgatgggcag catttctttt ggcattgcct tggtggcggg
     8221 tagaaatcgc gtgcccatgc cggcgacggg gaaaacggct ttgcggattg gtttcatgcg
     8281 ttgcggctgg atattagcgg gtgacttgct ttgtttcgag tgcgggaccg aaggtgacat
     8341 tttgcgtgtt gctttcctgc aggagctgtc gcggttcgcg gccctgctca cttggagggg
     8401 cgccgaaaaa accctggcca taagccagca gaatcaggtt ggcggccaga acaagtaaaa
     8461 acaggaatcg cattgccgct gacggaagaa gttgattgcc ttcagtgtaa agcgaattgg
     8521 ctggctcgca aagagccttg cccaagggcc agcaccgcca gaccgtcaag cacaggcgac
     8581 ggcagccatt caatgggcgt ttcatgcagg cccatgcgtt tctgtgcggc tgtcagcagg
     8641 cgctgggttt catctttcac aatgggccat gctccgccag agctgtaaac gcggggcgcc
     8701 tggccggaaa cctccagccc ggtcagccat tgccgcagaa ccgctccggc ctgggccgcg
     8761 gcgatgccgg tgacaatagc ctgatgagta ttggtaggat aggcggcggc cgtgccgctg
     8821 gcttcgggca gattcgcggt tccttcgacc agggacgagc gcattagcgt tgcgcctggg
     8881 aaaatgagcc ctcccaaaaa cagccaattg ttggactgcg gtgtgtcggt cgatggcgat
     8941 tggacagcgg tgtcaatggc gagcgtgtcg atggtggttg cggtgccgaa actggcaagc
     9001 atcagaggcg gttgcgaagt ttgaaggcga aca
//
LOCUS       DATCXU010000458         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_364572,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000458 DATCXU010000000
VERSION     DATCXU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364572"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VIR04_10225"
     CDS             <1..431
                     /locus_tag="VIR04_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEY9461166.1"
                     /translation="SVSLTGRNPEGSKSSQQRIQLNEDGEFGVLVGMQSPFHKSRSVV
                     AITGTDPRGLSSVLDALEDPGKVRLIQDEMVLVRGDKIEAVRTGGQYAVGNLPWYARI
                     WVVAIKHPILLALFGIIAGILVAIGIFLSLQSISSRRRGV"
     gene            435..1637
                     /gene="bcsZ"
                     /locus_tag="VIR04_10230"
     CDS             435..1637
                     /gene="bcsZ"
                     /locus_tag="VIR04_10230"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HEY9461167.1"
                     /translation="MAGTFARIFGVLFALLSGLILPPALAASSPMEQAGFGSGACTPW
                     SRWETFKNKFISADGRVIDLDSADARTVSEGQAYGLFFALVANDRREFDKILDLTQNI
                     LAQGDLTKHLLAWLWGKRSDATWGVIDSNSASDADLWFAYVLLQAGQTWHDRRYTALG
                     ALLAKNILSQEVARVPGLGWAILPGQAGFHLSDDVWRLNPSYVPLQVLRALASEPHDR
                     AKWRRVIESSRRLLVETAPHGFSPDWVQYRAVKSKSGFTPDVATHAEGSYNAIRVYLW
                     AGMLAGNDPDAARLLTVFRPMADYVERHGAPPEKVDTLTGKPGQNAGNAGFSAALVPF
                     LQTLGKPELAKAQAARVAQLEAHAPLGYYSQVLSLFGLGWYAGRFRFRADGSLWLAWN
                     QSCAGISR"
     gene            1595..>2424
                     /locus_tag="VIR04_10235"
     CDS             1595..>2424
                     /locus_tag="VIR04_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein"
                     /protein_id="HEY9461168.1"
                     /translation="MAGVEPVLRWHFAMMGAQPSLGQLTAVLAICIVCLPQACPASAT
                     DSVVAQLIASARFWSDNQRDDLAIQQVRKALLIAPDNPDALGALGLIEVRLNHMVDAT
                     RLLSRLTTLSPNSDATRELYYAYQVAGPQKQEFATIRRLSNTDQTAEAVRRLKALFSK
                     GPPQGDLAAEYFDVLAQNPPDRAAAIAALLQVVARRPENLDAALTLASLLNRETATRM
                     EAAEIVSRVFKNPRANRSSVLNVWRRVLRAAGDDPAYLEQLRAYLAVAPDDVEFKELL
                     V"
BASE COUNT          468 a          637 c          749 g          570 t
ORIGIN      
        1 attcggtcag cctgacgggc cggaatcccg agggctccaa gtccagccag cagcgtattc
       61 agttaaacga ggatggcgag tttggcgtgc tcgtgggcat gcagtcgccc tttcacaagt
      121 cgcgcagcgt ggtcgccatc acgggcacag atccgcgtgg cctgtcgtcg gtgctggacg
      181 cattggaaga tccgggcaag gttagattga tacaagacga aatggtcctg gtgcgcggcg
      241 acaagataga ggctgtgcgc actggcgggc aatatgcagt cgggaattta ccgtggtatg
      301 cccgaatctg ggtcgttgcc atcaagcatc ccatattgct tgcgttattt ggaataattg
      361 ccggcatttt ggtggcgatt ggcatctttc tgagtttgca gtcgatttcg tctcgaagac
      421 gcggagtcta gagcatggcc gggacttttg ccagaatttt cggggtgctt tttgctttgc
      481 tttcggggct tatcctcccg cctgcgctgg ccgccagttc gccgatggag caggcgggtt
      541 tcggttccgg cgcatgcacg ccgtggtcac gctgggaaac attcaagaac aaatttatca
      601 gcgcggatgg gcgcgtgatc gacctcgatt cggctgacgc aagaaccgtc tctgaaggcc
      661 aggcgtatgg tttattcttc gcgctcgtgg ccaacgatcg gcgcgagttc gacaagattc
      721 tggatttgac tcagaatatt ctggcgcaag gggatttgac caagcacttg ctggcctggt
      781 tatggggcaa gcgcagcgat gcgacctggg gcgtcatcga cagcaactcg gcatcggatg
      841 ctgacctttg gttcgcttat gtcttgctgc aggctggcca aacctggcac gaccggcgtt
      901 acacagcatt gggcgccttg cttgccaaga atatattaag tcaggaggtc gctcgcgtgc
      961 cgggtttggg ctgggctatt ttgccgggtc aggcgggttt tcacttgtct gatgatgtct
     1021 ggaggcttaa tcccagttat gtccctttgc aagtgctgcg cgcactggcg tccgagccgc
     1081 acgatcgcgc aaagtggcgc cgtgtgattg aatcttcacg gcgcttgctt gtggaaacgg
     1141 caccgcatgg tttttcgcca gattgggtgc agtatcgtgc ggttaaaagc aagtctggct
     1201 ttacgccgga tgtggccact catgccgagg gcagctacaa cgcaatccgc gtttatttat
     1261 gggcgggcat gttggcgggt aatgaccccg acgccgccag attgctgacg gtgtttcggc
     1321 ccatggccga ttacgttgaa cgacatggag ctccgcccga aaaagtcgat acattaacag
     1381 ggaaaccggg tcaaaacgcc gggaacgccg ggttctcggc agcgctcgta ccgtttcttc
     1441 agacgctggg caagccagag ctcgccaagg cccaggcagc ccgcgtcgca caattggaag
     1501 cccacgcgcc attgggttat tacagtcagg ttttaagcct gtttggactt ggctggtatg
     1561 caggccggtt tcgctttcgt gcagatggaa gcttatggct ggcgtggaac cagtcttgcg
     1621 ctggcatttc gcgatgatgg gcgcacagcc ttcattgggc caattaacgg ctgtgctggc
     1681 gatttgcata gtgtgtttgc cccaagcttg cccggcgtcg gcaacggaca gtgtcgttgc
     1741 gcagttgatc gccagtgcgc gtttttggag tgacaaccag cgggacgatc tagccatcca
     1801 gcaagttcga aaagcgttgc tgattgcccc cgataatccg gacgcgctgg gcgccctggg
     1861 tttgattgag gtgcgtttga accatatggt cgacgcaacc cggctgttat ctcgcttgac
     1921 gactcttagc ccgaattccg acgcgacgcg tgaactctac tatgcctatc aggtggctgg
     1981 accgcaaaaa caggaatttg ccacgattcg ccggttaagc aatacggatc agacggccga
     2041 agcggtcagg cgccttaaag ctctgttttc aaaagggcct ccgcaaggcg atttggcggc
     2101 cgaatacttt gatgtgctgg cgcaaaatcc gcctgatcgg gcggcggcca ttgccgccct
     2161 gctgcaagtg gttgcgcggc gccccgaaaa tcttgacgca gcgctgacat tggcgagtct
     2221 attaaatcgc gaaacagcca ctcgcatgga ggcggctgaa atcgtcagcc gggttttcaa
     2281 aaatccgcga gcgaaccggt cttccgtttt aaatgtctgg cgccgggttt tgcgcgcggc
     2341 aggcgatgac cctgcctacc ttgagcaact gcgcgcttat ttggcggtgg cccccgacga
     2401 cgttgaattc aaggaactat tggt
//
LOCUS       DATCXU010000459         5776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_46995,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000459 DATCXU010000000
VERSION     DATCXU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46995"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1337
                     /locus_tag="VIR04_10240"
     CDS             294..1337
                     /locus_tag="VIR04_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY9461169.1"
                     /translation="MKKRLILATLAAFLLSSAASAQELPKTHVKVVGYLSNVPAYTQF
                     EKPFWTQTVPKLSNGRITADIKSFDEMGLKGPEILRLMSQGVIEFGTATLSYFASDNP
                     INEAVDLAGTAPDAKTERIITDAFEPVYAKRLAKDNIKLLGIATNAAQVVFCNTDIKG
                     LADLKGKKVRTSSRTQADFVEALGGSSVNMAFAEVVPALQTKVLDCAITGSLSGFTAK
                     WYEVSTNLLELPINWNPIIYAVNLKAWQKLDPKVQTFIQTNLKTMIDSIWDDAARQTQ
                     EGYDCNTGAPACKAAVKGKMKLVAPSAADQELLKKLTSTAVVPKWAARCSADCVQSFN
                     ETIGKKLDIVAHK"
     gene            1370..1990
                     /locus_tag="VIR04_10245"
     CDS             1370..1990
                     /locus_tag="VIR04_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY9461170.1"
                     /translation="MAHSYRFPLLTSMLRKAEVFSRVAAWAGGAVMLASVFLICFDVI
                     GRKVLHFSTNSSNELSGYAFAISTSWALAFGLLQRINVRVDVLYRLLPVRVMAVLDWC
                     STVMMGVFMGYLTYYAYGVAQTSWVRDAAASTTLGTPLWIPQSLWVLGMVWMCIVLVL
                     MLVRASVALATGDLATVNALCGQRTAQEEAAEEAEAGKRMVEGKSA"
     gene            1987..3273
                     /locus_tag="VIR04_10250"
     CDS             1987..3273
                     /locus_tag="VIR04_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY9461171.1"
                     /translation="MIATTFILLLVLIGLSIPVAAALGVLGLISDQLFSMLPLSRAIG
                     EIAWSTSNEFLLVAIPLFILLGEILLRAGFAGRMYDAMSLWLSWLPGGLMHANIGACA
                     LFAATSGSSVATAATVGTVAMPQIERHGYNEPLFLGTIAAGGTLGILIPPSINLILYG
                     VLTSTSVPKLYLAGFIPGIVLALTFMVTVMIACAIKPEWGGHRIKASWGRRFASLADL
                     LPPLGLFLLVVGSIYAGVATATEAAALGVLGALVLAAFTGRLSIAMLRESFEGTMRTT
                     VMIMLIVIAAAFLSFVMSATGLTTILTDAVIGLHLAPEWMLLIVVIFYLILGCLMETL
                     SMMITTIPIIAPIMIAMGFDPVWLGIVIIVLVETALITPPVGLNLFVVQSLRKGGSMN
                     AVIIGSIPFVVALIFMVLLLAIFPDLALWLPRVFGD"
     gene            3318..4004
                     /locus_tag="VIR04_10255"
     CDS             3318..4004
                     /locus_tag="VIR04_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEY9461172.1"
                     /translation="MKLAFQLDAGAQSRRLDVDIQTLVVAGWSGRDVASIEHHIAELA
                     ELGIARPSALPLYYRIAHNQLTQEKKIQVVGEHSSGEVETFVFAVDGELFVSIASDHT
                     DRKLEAYSVAISKQACPKPVGTTAWRFADVADHWDELVIRSYIKEGGAVVLYQEGPLA
                     SLRTPANLIAGFADGAAVLPKGVGMSCGTVGAIGGIRPSTSFTMELFDPRGQRTLSHH
                     YEVEILPEVA"
     gene            4026..5006
                     /locus_tag="VIR04_10260"
     CDS             4026..5006
                     /locus_tag="VIR04_10260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY9461173.1"
                     /translation="MQYQNLGSAGVKVSRICLGCLTYGRPSDTHPWTLNEEQARPSIQ
                     RALEAGINFFDTANVYSAGSSEEILGRALRDFARRDEVVIATKVRSPMRKDPNGEGLS
                     RKAIMAEIDASLKRLGTDYVDLYQIHRWDYSTSIEETLEALHDVVKAGKALYIGASSM
                     YAWQFCKALYVAREHNWTRFITMQNHLNLLYREEEREMLGLCVSEGIGVLPWSPLARG
                     RLARPWGQQATERSGNDGFAQTLYAASEEADRRVVEAVGKVATARGVPYAQIALAWLL
                     QKPAIVSPVVGTSKPQHLDDALAALDVKLSADEITLLEAPYVPHQVAGFV"
     gene            complement(5028..>5776)
                     /locus_tag="VIR04_10265"
     CDS             complement(5028..>5776)
                     /locus_tag="VIR04_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461174.1"
                     /translation="PAVTRRLLLLLVLVFWEVAPRTGLVPTLFLPPLSNAFIVAYHDQ
                     SIYLSNLVVTLGEVAVSMAICCGGGVVLGAIIGSVPVLRHPLRTLFSSFYAIPLVILY
                     PLMAAWLGIGPESKVAFASLYGIVPAILASAAGIQSIDPQLALTARSMGATLPQRIAH
                     VTIPAAIPSILSAIRIGGSLAIVGVVVAEMLTSAAGLGFLITSYRTILDSSRVFAAIL
                     FVLAIALAFDALVRLLERHTARWSVADGQK"
BASE COUNT         1199 a         1495 c         1715 g         1367 t
ORIGIN      
        1 attgtgtttt gccggatcat taaaggtgat ccagccggtg cgcccttccc gctcgacaac
       61 cagccggctg gtgatctgct cctgcaacag gcgttctcta gtcatgttcg tcctttttct
      121 taaaataggt atatcgggcg atattagccg caacgaatcg tccgagctgg aaattgggcg
      181 tcaatcatcc ccatgcgcac cagattgcta gccatcaact tctttgccat gctattcttt
      241 tcacgttgat ggtttagctg aatcaagttg tctacctcaa gggagcctta cccatgaaga
      301 agcgccttat tctcgccaca ctcgctgcat ttctgttaag tagcgccgca tcagcccaag
      361 agctgccaaa gacccatgtc aaggtagtcg gctaccttag caacgttccg gcttacacgc
      421 agttcgaaaa accattttgg acgcaaacgg ttccaaagct gtcaaacggt cggatcacgg
      481 ccgatatcaa aagctttgat gaaatgggcc tgaaagggcc ggaaatttta cgtttgatgt
      541 cgcaaggtgt catcgaattc ggcacggcaa cgctttctta ttttgccagc gacaacccga
      601 tcaatgaagc ggtggatttg gccggcactg cgcccgatgc caaaaccgaa cgcatcatta
      661 ccgacgcgtt cgagccggtc tatgccaaac gtcttgcaaa agacaatata aagctgctcg
      721 gcattgcaac caatgcggct caggtcgttt tttgcaatac tgatattaaa gggctcgccg
      781 atcttaaggg caagaaggtg cgcaccagca gccgcactca ggccgatttt gtggaagcgc
      841 tgggcggctc aagcgtgaat atggcttttg ccgaagttgt gcctgcactt cagacgaaag
      901 tgctggactg cgcgattaca ggctcgttgt cgggttttac cgccaagtgg tacgaggtgt
      961 cgaccaatct gcttgagttg cccatcaact ggaaccccat catttacgca gtcaacctga
     1021 aggcatggca aaagcttgat cccaaggtgc agacgtttat tcagactaat ctgaagacga
     1081 tgatcgacag catctgggat gacgccgcgc ggcagaccca ggaaggttac gattgcaata
     1141 ctggcgcgcc cgcttgcaag gcggctgtta agggcaagat gaagttggtt gcgcccagcg
     1201 cggcggatca agagctgctt aagaaactga ccagtaccgc cgtcgtgccc aaatgggccg
     1261 cacgatgctc ggcagactgc gtccaaagct ttaatgaaac gataggcaag aagctggata
     1321 ttgtcgctca taaataaaca gtaatgattc gctcagaggg atggttttca tggcgcattc
     1381 ctatcgtttt cctttgttga catctatgtt gcgcaaggcc gaggtgtttt cacgcgttgc
     1441 cgcctgggcg ggtggcgcgg tcatgctggc cagcgtcttt ctgatttgtt ttgacgtgat
     1501 agggcgcaaa gtcctgcatt tcagcaccaa cagttccaat gagctttccg gctatgcctt
     1561 tgccatcagt acatcgtggg cgctggcgtt tggactgctg cagcgcataa atgtacgtgt
     1621 cgatgttctg taccggctat taccggtcag ggtgatggcc gtgctggatt ggtgttctac
     1681 cgtaatgatg ggcgtattca tggggtattt aacctattac gcttacggcg ttgcgcagac
     1741 ttcctgggtg cgcgatgccg ctgccagcac taccttgggt acgccattat ggatacccca
     1801 aagcttgtgg gtgctgggca tggtatggat gtgcatcgtg ctggtcctga tgcttgtgcg
     1861 cgcctcggtc gcgctggcga cgggcgatct ggccacggtc aacgccctct gcggccagcg
     1921 cacagcgcag gaagaagcgg cggaagaggc cgaggccggc aaacgcatgg tcgaggggaa
     1981 atcagcatga tcgcgactac cttcatactg ctcctggttc tgatcggatt aagcattccc
     2041 gtagccgccg cactgggtgt gctgggcctg atcagcgatc agcttttttc gatgctgccg
     2101 ctatcgcgcg cgataggaga aattgcctgg agcaccagca acgaattttt gctggtggcg
     2161 atccctttgt ttattttgct gggtgaaata ttgctgcgcg cgggctttgc cggacgcatg
     2221 tacgacgcca tgagtctgtg gttatcgtgg ttgccaggcg ggttgatgca tgcaaacatt
     2281 ggcgcttgtg cgctttttgc cgcaacgtcg ggctccagtg tggcaacggc cgcgacggta
     2341 ggaactgtag ccatgccgca aatcgagcgc catgggtata acgagccgct ttttttggga
     2401 accatagcgg cgggcggcac attgggcatt ttgattccgc cgtctattaa tctgattttg
     2461 tacggcgtat taaccagcac ttcggtgccg aaactgtatc tggcgggatt tattccgggc
     2521 attgtgctgg ctcttacgtt tatggtcaca gtcatgattg cgtgcgccat aaagccggaa
     2581 tggggagggc accgcatcaa ggcgtcttgg ggacgccgct ttgccagtct ggccgatcta
     2641 ttgccgcctc tgggattgtt tctgcttgtc gtcggctcca tctacgccgg ggtggctacg
     2701 gccaccgaag cggcggcgct gggcgtactc ggagcgctgg ttttggcggc ttttacgggg
     2761 cgcttgagta ttgccatgtt gcgcgagtcg tttgagggga cgatgcgcac tacggtgatg
     2821 atcatgctta ttgtgatcgc cgccgcattc cttagcttcg ttatgtcggc gacggggctt
     2881 accaccattt tgaccgacgc cgtcattggg ctgcatctgg cgccggaatg gatgttgctg
     2941 atcgtcgtca tcttttacct tatcctcgga tgcctgatgg aaaccttgtc gatgatgatc
     3001 acgacgatac ccatcatcgc acccatcatg atcgccatgg gatttgatcc tgtctggctg
     3061 ggcatcgtta ttatcgtgct ggtggaaacg gctcttatta cgccgccagt gggtttgaac
     3121 ttgttcgtag tgcaaagctt gcgcaagggt ggttcgatga atgctgtcat tattggcagc
     3181 attccttttg ttgtcgctct gatttttatg gttttgctgt tggccatatt ccccgatctt
     3241 gcgctgtggt tgcccagggt gttcggcgat taggtcatat ttgtgtattc caaaattttt
     3301 gcgggcgttg ctctattatg aaattggctt tccaacttga tgcaggcgct cagtcacgcc
     3361 gcctggatgt tgatattcaa accttggttg ttgcgggttg gtctggccgt gatgttgctt
     3421 ctatcgagca tcacattgcg gaattggctg agctgggcat tgctcgccca agcgcgttgc
     3481 cgttgtatta ccgaattgcg cacaatcaat tgactcagga aaaaaagata caagtcgttg
     3541 gcgagcattc gtcgggcgaa gtggaaacat ttgtcttcgc cgttgatggc gaattattcg
     3601 taagcatcgc ttctgaccat accgatcgca agctcgaagc gtatagcgtc gcaatatcca
     3661 aacaagcgtg tcccaagccg gtggggacta cagcgtggcg ctttgccgat gtcgcggatc
     3721 actgggacga gcttgttatc cgctcttata ttaaagaagg gggcgccgtt gttttatatc
     3781 aggaagggcc tctcgcgtcg ttgcgcacgc cagccaatct gatcgcgggg tttgccgatg
     3841 gcgccgcggt gctgcctaaa ggggtcggaa tgtcctgcgg gacggtgggt gcgattggcg
     3901 gcatacggcc atcgacctcg tttacgatgg agttgttcga tccgcgcggt cagcgcacgc
     3961 tcagtcatca ttacgaagtc gagatattgc ccgaagtggc ctgacgacat aaaggagcca
     4021 gccttatgca gtaccagaat ctgggcagcg cgggggtaaa ggtctcgcgc atttgtcttg
     4081 gttgtttgac gtatggcagg ccatcggaca cgcacccatg gacgctaaac gaagagcaag
     4141 cccggccatc cattcagcgc gcgctcgagg cgggcatcaa ctttttcgat acggccaatg
     4201 tctattcggc gggttccagc gaagaaattt taggccgcgc attgcgcgat tttgcccggc
     4261 gcgacgaggt tgtcattgca acgaaggtgc gcagcccgat gcgcaaagat ccgaacggcg
     4321 aggggctttc gcgcaaagcc atcatggccg aaattgatgc aagcctgaag cgcctgggca
     4381 ctgactacgt tgatttgtac caaattcatc gctgggatta ttcaacgtcc atcgaggaaa
     4441 cgcttgaagc attgcacgat gtcgtgaaag cgggtaaggc gctttatatc ggtgcatcgt
     4501 ccatgtacgc ctggcaattc tgcaaggcgc tgtacgtggc gcgcgaacat aactggacgc
     4561 gtttcatcac gatgcaaaat catttgaatt tgctttaccg cgaagaagag cgcgagatgc
     4621 tgggtttgtg cgtgtctgaa ggaattggcg tgctgccctg gagcccgctt gcacgcgggc
     4681 ggttggcgcg accctggggc caacaggcta ccgagcgctc cggcaatgat ggttttgcgc
     4741 aaacacttta tgcggccagc gaagaagcgg accggcgcgt ggtcgaagca gttggaaagg
     4801 ttgctacggc gcgcggcgtg ccttatgcgc aaattgcgct tgcctggctt ttgcaaaaac
     4861 cagccattgt ctcgccggtg gtcggaacgt cgaagccgca acatcttgac gacgcattgg
     4921 ccgcgcttga tgtcaaatta agcgccgacg aaattacttt gctggaagcg ccttacgtgc
     4981 cgcatcaggt ggccggattc gtttgaaccc ctggcacgca tacgccgtta tttttgcccg
     5041 tcggcaacgc tccagcgtgc ggtgtgtcgt tcaagcagcc gcaccaaggc gtcaaacgcc
     5101 agcgcaatgg ccagcacgaa caggatggcg gcaaacacgc gcgaggagtc caggatggtt
     5161 ctgtagctgg tgatcagaaa tcccaggccg gcggccgagg tcagcatctc ggccaccact
     5221 acgcccacga tggccagcga accgccgatg cgtatggccg acaaaatgga agggatggcc
     5281 gccggaatcg taacgtgcgc aatgcgctgg ggcagcgtgg cgcccatgct gcgggcagtc
     5341 agtgcaagct gggggtcaat gctctgaatg cctgccgcgc tggccagtat ggcaggcacg
     5401 atgccgtaca agctggcgaa agccaccttt gattccgggc cgatgcccag ccaggcggcc
     5461 atcagcgggt acaagatgac cagcggtatg gcgtaaaagc tggaaaacag ggtgcgtagc
     5521 ggatggcgca ggacgggcac gctgccgata atggcgccca gcacgacgcc gcccccacag
     5581 caaatcgcca tcgacacggc gacctcgccc agtgtgacca caaggttgga caggtagatg
     5641 gattggtcgt gataggcgac gatgaacgcg ttgcttaagg gaggcaaaaa caacgtgggc
     5701 accagccccg ttctgggcgc gacctcccag aagacgagca ccagcaaaag tagaaggcgt
     5761 cgcgtgacgg cgggcg
//
LOCUS       DATCXU010000460         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_990000,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000460 DATCXU010000000
VERSION     DATCXU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990000"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIR04_10270"
     CDS             <1..339
                     /locus_tag="VIR04_10270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY9461175.1"
                     /translation="AECGGVMHCFTESWEIAQAAMEMNFYISMSGIVTFKNAKDVQDV
                     AAKVPLERLLIETDSPYLSPVPHRGVLNDPSKVALVAKKVAELRGVPVAEIARHSTEN
                     FFRLFTEAAV"
     gene            534..1427
                     /locus_tag="VIR04_10275"
     CDS             534..1427
                     /locus_tag="VIR04_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY9461176.1"
                     /translation="MNGQQLTGNCRSWVLCLATGLIGMLASLAAYAANPGAWWADIAQ
                     NRAANVQSMLAQGADPNELGPEGQPAIMAAIRHNAWNAYDVLAASPKIEVNLTNVSDE
                     TPLMYLAIVGETGRAQNLINRGAHVNRLGWTPLHYAASKGQLAMVKLLIADKAIINAP
                     APDGTTALMMAAFAGSEPVVRVLLAAGADVTTRNLKAQDAADWALLSKHGALARKIRV
                     LIDDKLAQRAALRARAKSGTSTDAGPGNTGADSLDAKIKDLEKSAPEQPSGNEAGRQG
                     AKDSNSEDHSSRYFDLNRFNK"
     gene            complement(1475..2281)
                     /locus_tag="VIR04_10280"
     CDS             complement(1475..2281)
                     /locus_tag="VIR04_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEY9461177.1"
                     /translation="MRRRARALRLVTIGFAAGLVAACASGPSTNISLIPANKVAQETA
                     KEGVFVQPVQWKHKKPGCKGECPAIELHSIVFPGIAKFSELIDHALATMTGVGSKGLP
                     PYDTIAEYQDYFWKTAGPRDSATLSAKVRYRNKNLTVVEMNTYQYYTGAAHGISATQF
                     LNWSNATKRVLGIADILEPGKHDAYVQALKEVHGRWLANNADARHDPQTYNRIWPFQA
                     SDNFAFTDHGLAVKYDSYQIAPYYFGQPELLIPYSALAGILRSEFLPAAS"
     gene            complement(2284..>2386)
                     /locus_tag="VIR04_10285"
     CDS             complement(2284..>2386)
                     /locus_tag="VIR04_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide oxidoreductase"
                     /protein_id="HEY9461178.1"
                     /translation="LRAYGRAREINVAPTVNEKSRELLFGQSAKSVR"
BASE COUNT          543 a          601 c          673 g          569 t
ORIGIN      
        1 gcagagtgcg gcggggttat gcactgtttt accgagtcgt gggaaattgc gcaagccgcc
       61 atggaaatga atttttatat ttcgatgtcg ggcattgtca cgttcaaaaa tgccaaagac
      121 gtgcaggacg tggcagccaa agtgcctttg gaacgtttgc tgatcgagac ggactcccct
      181 tacttgtcac cggtgcctca tcggggggtg ttgaacgatc catccaaggt agcgctcgtt
      241 gcaaaaaaag tagcagaatt gcgtggcgtc cctgtcgccg agatagcacg gcattcaact
      301 gaaaactttt tccgattgtt caccgaagcg gctgtataac ttaaagtaca ctcgttcgaa
      361 ttagccttga cttttgatgt ctgcgctatt tggcttacag gtttacgtct taattattat
      421 gttatatatc aaatagttac atcatataat taatctattt tagttaaagt aaactgttta
      481 aagtaatttc tttaaagtaa tttatattta gcatacattt aaggtgcatt cttatgaacg
      541 gacaacaatt aacgggtaat tgccgctctt gggtcttgtg cctggcgaca gggttgattg
      601 gcatgctggc aagtctggcg gcttatgctg ccaatccggg cgcctggtgg gcggatatcg
      661 cccaaaatcg cgctgccaac gtgcagtcca tgctggcgca aggcgccgac ccgaacgaat
      721 tgggccccga agggcaaccg gccatcatgg cggctattcg ccataacgca tggaatgcct
      781 acgatgtgct ggccgcctcg cccaagattg aagtcaacct gacgaatgtg tcggatgaaa
      841 cgccgctgat gtatctggct atcgtcggcg aaactggaag ggcgcaaaat ctgataaatc
      901 gcggcgcaca tgtgaatcgg ctgggctgga cgcctttgca ctacgctgcg tccaaaggcc
      961 agcttgccat ggtgaagctg ctgatcgcag ataaggcgat tatcaacgcg ccggcgcctg
     1021 acggcacaac cgcgctgatg atggctgcct ttgccggcag tgagccggtt gtccgggtat
     1081 tgctcgcggc gggggcggac gttactacgc gaaatctgaa ggcgcaagac gcggctgatt
     1141 gggccctttt aagtaaacat ggtgcgctgg cgcgcaaaat ccgggttttg atcgatgaca
     1201 agcttgcaca gcgtgccgct ttgcgcgcca gggccaagtc gggaacatct acggatgcgg
     1261 ggcccggcaa caccggcgcc gattcgctcg acgccaaaat taaagacctc gaaaaatccg
     1321 ccccggagca accatccggc aatgaggctg ggaggcaggg cgcgaaagac tcaaattccg
     1381 aagatcattc ttcgcgttat ttcgatttga atagatttaa caaatagcgc ttggtggctg
     1441 gcgcgtaccg acaaagtgcg cgccaaagct ttgcctagct tgccgccgga agaaattccg
     1501 atctcaggat cccggccagg gcgctgtagg gtatgagcaa ctctggctgg ccgaaataat
     1561 agggcgcaat ctgataggaa tcatacttga ccgccaggcc atgatcagta aaggcaaagt
     1621 tatcgctggc ctgaaagggc cagatgcggt tataggtttg aggatcgtga cgggcgtcgg
     1681 cgttattggc cagccagcgg ccatgaactt ctttcaacgc ctggacatac gcatcgtgct
     1741 tgccaggctc aagaatatcc gctatcccca gaacacgttt tgtggcgttg ctccaattca
     1801 ggaactgggt cgctgaaatc ccatgagccg cgccggtgta atactgatag gtgttcattt
     1861 ccacaaccgt cagattcttg ttgcggtatc ggaccttggc ggaaagtgtg gcggagtcgc
     1921 ggggcccggc cgttttccag aaataatcct ggtattcggc gattgtgtca tagggaggca
     1981 gtcctttgct gcccacgccg gtcattgtcg ccagcgcatg atcgatgagt tcgctaaatt
     2041 tggcaatgcc tggaaaaacg attgaatgca gctcgattgc cgggcattcc cccttgcagc
     2101 cgggcttttt gtgtttccac tgcactggct gcacgaacac gccttctttg gccgtttctt
     2161 gcgccacttt attcgccggt ataagcgaga tattggttga tgggccgcta gcgcacgcgg
     2221 caacgagacc ggcggcaaaa ccgatggtaa ccaggcgcaa agcgcgagca cggcgccgca
     2281 tggtcatctc acggatttgg cgctttggcc aaacagcaat tcacgcgatt tttcatttac
     2341 ggtgggggca acatttattt ccctggccct gccgtaggcc cgcagt
//
LOCUS       DATCXU010000461         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_300152,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000461 DATCXU010000000
VERSION     DATCXU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300152"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="purH"
                     /locus_tag="VIR04_10290"
     CDS             <1..605
                     /gene="purH"
                     /locus_tag="VIR04_10290"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019849.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY9461179.1"
                     /translation="AATALAITGQFVEVVLAPGYEPEALAIFASKKNVRVLQVPAGQA
                     HNTFDVKRVGGGWLVQSPDDLYVQQNQLKTVSKLAPTAQQIQDLMFAWKVAKYVKSNA
                     IVFAANGMTLGVGAGQMSRIDSARIASIKAENAGLSLAGSAVASDAFFPFRDGLDVVA
                     DAGATCVIQPGGSMRDAEVIAAADERGIAMMFTGARHFRH"
     gene            605..1150
                     /gene="ruvC"
                     /locus_tag="VIR04_10295"
     CDS             605..1150
                     /gene="ruvC"
                     /locus_tag="VIR04_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY9461180.1"
                     /translation="MRVLGIDPGLRRTGFGVIDVLGTQLQYVSSGTIVAPTDQPMAQR
                     LKVILDNLREIVQTNQPAVAAIEKVFLNTNPTSTLLLGQARGAALCAMADSGLFVHEY
                     TALQIKKAVVGNGHAAKEQVQKMVQHLLALNGVPAADAADALACAICHANVSPLARRL
                     KVAGGLDPDKESRLRGGRLIG"
     gene            1178..1753
                     /gene="ruvA"
                     /locus_tag="VIR04_10300"
     CDS             1178..1753
                     /gene="ruvA"
                     /locus_tag="VIR04_10300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY9461181.1"
                     /translation="MIGRITGTLLEKTPPVVCIDVGGVGYEIDVPMSTLYKLPDNGAT
                     VTLFTHLAVREDAHTLYGFSSASERAAFRALIKVTGIGARTALAVLSGMTVEELASAI
                     TRQETGYLTRVPGIGKKTAERLLLELRGKLGADLGSSAGAVSPGREDILNALLSLGYS
                     NNETQMALKNLPDGIDVAEGIRMALKSLARR"
     gene            complement(1736..2707)
                     /locus_tag="VIR04_10305"
     CDS             complement(1736..2707)
                     /locus_tag="VIR04_10305"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEY9461182.1"
                     /translation="MSVVLPVYEDVVRASALLKGVAHHTPVLTSETANKRSGASLYFK
                     CENFQRVGAFKFRGGYNAVANLSPAQRKAGVVTFSSGNHAQAIALAARLQGVAATIVM
                     PRDAPAAKKAATLGYGAKVVEYDRYKEDRAQIAQGLVEQYGLTLIPPFDHPDVIAGQG
                     TAAKELIEQVGGLDQLFVCLGGGGLLSGSLLAAHALSPNCAVYGVEPERGNDGQQSLR
                     QGKIVRIAPPETIADGAATASLGLLTFPIIQQYARDILTVSDEQLIHAMRFFAERMKM
                     VVEPTGCLSAAAVFNQCVPVRGQRIGVIVSGGNVDMDVYSKLLASGQ"
     gene            2787..>3656
                     /gene="ruvB"
                     /locus_tag="VIR04_10310"
     CDS             2787..>3656
                     /gene="ruvB"
                     /locus_tag="VIR04_10310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY9461183.1"
                     /translation="MAIHSDSLSSTPPPARVVTPHSSSPNEESIERALRPRTLSEYIG
                     QKRVCEQLDIFITAARNRGEALDHLLLFGPPGLGKTTLAHIVAHEMGVQLRQTSGPVL
                     ERPGDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDFQIDILIGEGPAARSVK
                     IDLQPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYNADELTHIVTRSAHLLNVTATHE
                     GAIEIARRARGTPRIANRLLRRVRDYAEVRAGGNIDASVAAAALTMLEVDPQGLDLMD
                     RKLLEAIIHKFDGG"
BASE COUNT          801 a         1091 c         1047 g          717 t
ORIGIN      
        1 atgcggccac cgcgctggcc atcaccgggc agttcgtgga agtggtgctt gcgcctggct
       61 atgaacccga agcgcttgcc atcttcgcct cgaaaaagaa cgtgcgtgta ctgcaagtgc
      121 cggcgggaca agcccacaac acgttcgatg taaaacgcgt gggcggcggc tggctggtgc
      181 aaagcccgga cgacttgtac gttcagcaaa accagctcaa gacggtgagt aaactcgccc
      241 ccacagcgca gcaaatacag gatttgatgt tcgcctggaa agttgccaaa tacgtgaaat
      301 ccaatgccat cgtgttcgcg gccaacggta tgactcttgg cgtgggcgcc ggtcaaatga
      361 gccgcatcga ctcggcccgc attgcttcca tcaaggctga aaatgccggc ctgagcctgg
      421 ccggctccgc cgtggcctcc gacgcgttct ttccgtttcg ggacgggctt gacgtggtgg
      481 ccgatgccgg agcgacctgc gtgatccagc ccggaggcag catgcgagac gctgaggtca
      541 ttgcggcggc cgatgaacgc ggcatcgcca tgatgttcac cggcgcgcgc cacttccgac
      601 actaatgcgc gttttaggca tcgatccggg attgcgccgc accggctttg gcgtcatcga
      661 cgtactggga acgcaattgc agtatgtcag tagcggcact atcgttgcgc caacggatca
      721 gccgatggcg caacgcctga aagtcattct cgataatctg cgtgaaatcg tgcagacgaa
      781 tcagcctgct gtggccgcca tcgaaaaagt atttttgaac acgaatccca cttcgaccct
      841 tctgctgggg caggcgcgcg gagccgccct gtgcgccatg gccgatagcg gattgtttgt
      901 gcatgaatac acggcgctgc aaatcaaaaa ggctgtcgtc ggcaacggac atgccgccaa
      961 agaacaagtg caaaaaatgg tgcagcattt attggcctta aacggcgtac ctgcggccga
     1021 tgccgccgac gcgctggctt gcgccatttg ccatgccaac gtcagcccac tggcccgacg
     1081 cctgaaggtg gcgggtggcc tggacccaga caaagaaagc cgcctgcgtg gcggccgcct
     1141 gatcggttaa cgccaatact actgaaagga agctcccatg atcggacgaa tcaccggaac
     1201 attgctggaa aaaactcctc cggtcgtatg catcgacgtg ggcggcgtgg gctacgaaat
     1261 cgacgtaccg atgagcacgc tttataaact gcccgacaac ggcgccactg ttactctgtt
     1321 tacccacttg gccgtacgcg aggacgccca cacgctttac ggattttcaa gcgccagcga
     1381 acgcgcggca tttcgcgccc tgatcaaggt aacgggaatt ggtgcgcgca ccgccctggc
     1441 agtgctatca ggcatgaccg tcgaagaact tgccagcgcc atcacgcggc aggaaacagg
     1501 ttatttgacg cgtgtgccgg gcatcggcaa gaaaacggcg gaaagactat tgctggagtt
     1561 gcgcggcaaa ctgggcgccg atctgggatc gagcgccggc gccgtctcgc cgggccggga
     1621 agacatcctg aatgccttgc tttcattggg ctattctaat aacgaaacac aaatggccct
     1681 gaaaaacctg ccggatggca tcgatgtcgc cgaaggcatt cgaatggcgc tgaaatcact
     1741 ggcccgacgc taaaagcttg ctgtagacgt ccatatcgac attgccgccg ctgacaatca
     1801 cgccaatacg ctgcccgcga acagggacgc actgattaaa taccgccgca gcgcttaaac
     1861 agccggtggg ttcaaccacc atcttcatgc gctcggcaaa gaatcgcatg gcgtggatta
     1921 attgctcgtc gctaacagta aggatgtcgc gtgcgtattg ctgaatgata ggaaacgtga
     1981 gcaagcctaa agatgccgtc gccgcgccgt cagcgatggt ttcaggcggc gcgatacgaa
     2041 cgattttccc ctgccgcaac gattgctggc catcattgcc ccgttcaggt tcgaccccat
     2101 acacggcgca attcgggctt aacgcatgcg ccgccagcag cgaacccgac aacaaaccgc
     2161 caccgcccag acatacaaac agttgatcaa gaccgcccac ttgttcgatc agttcttttg
     2221 ccgcagtacc ctggcccgcg atgacgtcag ggtgatcgaa gggcggaatc agggtcaagc
     2281 cgtattgctc gaccaggcct tgcgcaattt gagcccgatc ttctttgtag cggtcgtact
     2341 ccacgacctt ggcgccgtac cccagcgtcg ccgccttctt ggcggcgggc gcgtcgcgcg
     2401 gcatgacgat tgtggcagca accccttgca gacgcgcggc cagcgcgata gcctgcgcat
     2461 gattgccgga agaaaacgtg accacgcccg ccttgcgctg agccggcgat aaattcgcca
     2521 ccgcgttgta gccgccccga aatttaaatg cgccgacccg ctgaaagttt tcgcacttga
     2581 aataaagact ggctcctgat cttttgttgg ccgtttccga agtcagcact ggcgtgtgat
     2641 gcgccacccc tttcaaaagg gcgctggccc ggaccacatc ttcatagaca ggaagaacga
     2701 cgctcatgac gactccgtta tttcaattat gcagtagtgt acgattgtta taaattttac
     2761 tgtaatgtcg ttatccagcg agcatcatgg ctatccactc cgattcgctt tccagcaccc
     2821 cgccgcctgc gcgcgttgta actccgcata gctcttcgcc caacgaagaa tcgattgaac
     2881 gcgccctgcg tccgcgcacc ttaagcgaat acatcggaca gaaacgggta tgcgagcagc
     2941 tcgatatatt tattacggct gcgcgaaatc gcggcgaagc gctggaccat ttattgttat
     3001 tcggcccccc gggactcggc aagaccacgc tggcgcatat tgttgcgcac gaaatgggcg
     3061 ttcagttgcg ccaaacttcg ggcccggtgc tggagaggcc cggcgatctg gcggccctgt
     3121 tgaccaacct ggaaaaaaat gacgtcttgt tcattgacga aattcaccgc ttgtcgcccg
     3181 tggtggaaga aattctgtac ccggcgctgg aagactttca aatcgacatt ctgattggcg
     3241 aagggccggc agcgcgcagc gtcaaaatcg acttgcagcc ctttacactg gtcggtgcaa
     3301 cgacgcgcgc cggaatgctg accaaccccc tgcgggaccg gttcggcatt gtgtcacggc
     3361 tggagtttta caacgccgac gaattgaccc atatcgttac ccgcagcgcg catttgctga
     3421 atgtgaccgc gacccatgag ggcgccatcg agatcgcgcg acgggcgcgc ggcacgccgc
     3481 gcattgccaa tcggctgctg cggcgcgtac gggactacgc agaagtccgc gccggcggca
     3541 acatcgacgc cagcgtggca gcggccgcgc taaccatgct ggaggtcgac ccccaaggcc
     3601 tggatctgat ggatcggaaa ctgctggaag ccattattca caaatttgat ggcggc
//
LOCUS       DATCXU010000462         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_129112,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000462 DATCXU010000000
VERSION     DATCXU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129112"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VIR04_10315"
     CDS             complement(<1..767)
                     /locus_tag="VIR04_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9461184.1"
                     /translation="MKFLLIASFPDSLVLFRGPLINALLAHGLQVHVAAPDLAEGSAV
                     RVRLENEGVHAHDIPLQRTGVNPLADTRTLFHLWKLMCVVRPDFVLGYTIKPVIYGSL
                     AARLAHVPKRFALITGLGYSFQGDGQRSVLRSLVQRLYSFALRGVNTTFFQNPDDDAL
                     FRRLKILQPFDTSLVVHGSGVNIDYYTVAPLPAAPRFLLIARLLGDKGIREYAQAARR
                     IRSRYPEVVFGLVGWVDENPNSVTQQELDNWIAEGTL"
     gene            complement(764..1507)
                     /locus_tag="VIR04_10320"
     CDS             complement(764..1507)
                     /locus_tag="VIR04_10320"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HEY9461185.1"
                     /translation="MNKFIIRFDDITPTMAWSKFFAIEKTMIDLNIKPIMGVVPLNKD
                     AQLMAEPEREDFWSIVKDWRAHGWTIAQHGYTHEYATTDPGILKINCGSEFSGLPYEE
                     QYRKLKAGKEILDANGVWQPVFMAPAHSFDRSTISALTSLGFKSLTDGYGVYPYMIES
                     ICALPQLFSSPIHFGFGVYTFCMHVNAMTMEEINNIDAFLRKNHRHFVSFDEALAMKG
                     YAAVSRTARYFSKLGLCTARGLRRKLQPL"
     gene            complement(1590..2831)
                     /locus_tag="VIR04_10325"
     CDS             complement(1590..2831)
                     /locus_tag="VIR04_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9461186.1"
                     /translation="MEPGKQGSGAKVENVDATCQDFKDMKKILYIGGFESDEKGAIGT
                     HTAGILRALDESDFFDLHLTNISSAKPHYLPRNLHEYYLPKPANKIAKIFAILKYALY
                     VKKIIKKIRPDFIYLRFDPFLTPLLTVRKTKSLVEYNDIFLDQILFTSKKKYWGYWGT
                     KFRNSGIYKRIIRSSERYTFARAHMVIAVTDGILKYCINIKPDANGIVLHNASNAFCD
                     ITAPSQNEALILSHVGTLTYWDGLEELIEAINFALVKAPETRIRLNIVGDGPLQPRLK
                     GIVHSYRLENVVRFYSSVTHDQALNFVQSSDVIPLLKTIYTYGLSPIKYYEAMASGCH
                     ILVSDIPCINEATEYVGTVVSYPLKIKEIGDKIIELSQKKEDIRKNRKRIQAYAFENH
                     TWDSRVQLLKEKILFLEAAHS"
     gene            complement(2786..4024)
                     /locus_tag="VIR04_10330"
     CDS             complement(2786..4024)
                     /locus_tag="VIR04_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461187.1"
                     /translation="MELFSTAWQFLSSVLIFAAGILVAISVGRRFNTTARRSLTLYGW
                     HTIFCIIATLYLVANGGDAIDYYKTALAGNVEFSVGTAAVSFITVFFTSVLGLSFLGA
                     SLVYQIAGFIGLLAFDASLQAATAEKSKYVRRLATLIVFLPSVSFWSSAIGKDSLSFM
                     AVGFALWAALNLKRRVWLMVASVAIMLLVRPHIAGMIVIAFAGSLIIKRNIPLARRLG
                     FGIATLVAATLIVPFAMTYAGLESAVTAQDLNNYIEQRQQQNLEGSASFDLAAMTPPM
                     QLFTYLFRPLPFEARTFFALAASLDNVILLMLFIAGGWSILKRYRPTIVGNRGFLWLY
                     SLLAWSLLAMTTANLGISLRQKWMFAPMMILLLISLIGRPRRKLNQARTLVRYSVPAP
                     HPDRASWNPVNRGAAPRWKM"
     gene            complement(4034..>5162)
                     /locus_tag="VIR04_10335"
     CDS             complement(4034..>5162)
                     /locus_tag="VIR04_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY9461188.1"
                     /translation="RLAAAGQKCGDQNTVISLMDRGRYGAKLEQANCTVHCLQMPRGR
                     ITLRGLLTLYSIIKSARPDVVQTWMYHADLIGGITARLAGVKAVVWGIRHANFDRDKN
                     AGSTPRIIKLCAWLSSTIPARIVCCSVNAAPLHKKIGYRANKIIVIPNGYELGELQPC
                     PESGRRIREEFKLDENAPLIGMVARFDPQKDHQNLIEALMLLRRQHRVFTCLLVGCDM
                     TPGNDHLMRRLQQAGVEKNVFLLGPRNDIPAIMNALDVHVLSSLGEAFPNVLAEAMAC
                     ATPCVTTNVGDASLIVGNTGWVVPARNAQSLSNAIALALDERRLQPLTWQRRRDDCRQ
                     RIVQNFSLKKMLASYHAVWADTAKCNRPFSSAPACGPSKFK"
BASE COUNT         1228 a         1319 c         1316 g         1299 t
ORIGIN      
        1 tccagcgtgc cttcggctat ccagttgtcc aactcctgct gtgtgacaga gtttgggttt
       61 tcgtcgaccc acccaaccag gccgaacaca acttccgggt agcgcgatcg gattcgccgc
      121 gccgcctgcg catactcgcg tattcctttg tcgccgagca atcttgcaat gagcaaaaag
      181 cgtggtgctg ccggcaatgg cgccaccgtg tagtagtcga tgttgacgcc cgagccatgc
      241 acaacgagcg acgtatcaaa aggttgtagg atcttcaggc ggcgaaatag agcgtcgtcg
      301 tcagggttct ggaaaaaagt cgtattaacc cctcgtagag cgaagctgta caagcgttgc
      361 accaggctgc gcaatacact tcgctggcca tccccctgaa acgagtatcc caggccggtt
      421 atcaatgcga agcgttttgg cacgtgcgct agccgcgccg ccagcgaacc gtatataacc
      481 ggcttgatcg tgtagcccag tacgaagtcc ggtcgcacga cgcacatcag tttccataga
      541 tgaaagagag tgcgggtatc agccagtggg ttcacaccgg tccgctgtag aggtatgtcg
      601 tgtgcatgca cgccttcgtt ttccaggcgc acacgcacag cgctgccctc ggccaaatcg
      661 ggagccgcca cgtgcacctg caacccgtgc gccaataagg cgtttatcaa gggaccccga
      721 aagagtacaa gcgagtcagg aaaactggcg ataagaagaa atttcataag ggctggagct
      781 ttctgcgcag gcctcgggcc gtgcacaaac ctaacttgga gaaatatcgg gccgtccgcg
      841 aaactgccgc gtaacctttc atcgcaagcg cttcatcgaa agaaacaaaa tgacgatgat
      901 tttttcgcag aaacgcgtcg atgttattaa tttcttccat tgtcattgca ttgacatgca
      961 tgcagaacgt atagacgcca aacccaaaat gtatgggcga tgaaaataat tgcggcaggg
     1021 cacatatgct ttcaatcatg tacggataga ctccatatcc gtccgtcagg cttttgaaac
     1081 caagactggt gagcgccgaa atagtcgaac ggtcgaacga atgcgcgggc gccataaaca
     1141 cgggttgcca aacgccattg gcgtccagga tttctttgcc cgcctttaat tttctatatt
     1201 gttcttcgta tggcaaaccg gaaaattctg atccgcaatt tattttcaag atacccggat
     1261 ccgttgtcgc gtactcatgc gtgtatccgt gctgtgcgat tgtccatcca tgcgcccgcc
     1321 aatctttcac tatgctccag aagtcttcac gctcgggttc agccatcagt tgtgcatctt
     1381 tatttaacgg cacgaccccc atgatcggct tgatgttcag gtcgatcatg gttttttcaa
     1441 tagcaaaaaa cttgctccac gccatagtcg gcgtgatgtc atcgaaacga atgatgaact
     1501 tattcatata tcgcacggat ccaatgaatg taaacggcga acgcgattaa ttgttttcaa
     1561 ctggtgatgg ttaaaaggcg gcggccccgt taactatgcg cggcttccag aaacaggatc
     1621 ttctctttta atagctgcac tctggagtcc cacgtatgat tttcgaatgc ataggcctgg
     1681 attctttttc tatttttgcg gatgtcttct tttttttggg acagttcgat gattttgtcg
     1741 cctatctctt taatttttaa cgggtagctt acgacggtgc cgacatattc cgtcgcttca
     1801 tttatgcaag gaatatccga caccagaatg tgacacccgg aagccattgc ttcgtagtat
     1861 ttgatcggcg aaagcccgta tgtatatatt gttttcaata gtgggatgac gtcgctgctt
     1921 tgaacaaaat tcaacgcttg gtcatgggtg acggaactgt agaaccggac tacgttttca
     1981 agccgatatg aatgaactat tcctttcagc ctgggttgca atggtccatc gccgactata
     2041 ttcaatcgta tccttgtttc gggggctttg accaaggcga aattaatggc ttcgatcagc
     2101 tcttccagtc catcccagta ggtcaaggtg ccaacgtggc tcaggataag cgcttcattt
     2161 tgtgatggag ccgttatgtc acagaatgcg ttgcttgcat tatgcagaac gatgccattg
     2221 gcgtccggtt tgatgtttat gcaatattta agaattccat ctgtcaccgc tatgaccatg
     2281 tgcgccctgg caaatgtgta cctttcacta cttctgatta ttctcttgta tattccggag
     2341 tttctgaact tcgttcccca ataaccccaa tatttctttt tgctcgtgaa aagaatctga
     2401 tcgaggaaaa tgtcgttgta ctcgacgaga gattttgttt ttcggactgt caataggggc
     2461 gtcagaaaag ggtcgaatct caagtatata aagtcagggc gtattttttt tatgattttt
     2521 tttacatata aggcatattt cagtattgca aatattttgg ctattttatt tgcaggcttg
     2581 ggtagatagt attcatgaag gtttctgggc aaataatgcg gcttcgccga gctgatgttt
     2641 gtcaggtgaa gatcaaaaaa atccgactca tccaaagctc ttaatatgcc tgccgtatgc
     2701 gtgccgatag cgcctttttc atcgctttca aatccaccta tatacaatat ttttttcatg
     2761 tctttgaaat cttgacaagt cgcatctaca ttttccacct tggcgccgct cccctgttta
     2821 ccgggttcca acttgcccgg tcaggatgcg gggctgggac tgaataacga acaagcgtac
     2881 gtgcctggtt tagtttcctg cgcggtcttc cgatcaacga tatcagcagc agaatcatca
     2941 tcggtgcaaa catccacttc tgacgcaacg agatacccag gtttgcggtg gtcatggcca
     3001 acaggctcca tgccagcagc gagtacagcc acaggaaacc gcgatttccg acgatggtcg
     3061 gcctgtaccg tttcaaaata ctccagccgc ctgcaatgaa cagcatgagc aagatgacat
     3121 tatccaggga cgccgccaac gcaaagaagg ttctggcttc gaatggaagc ggccgaaaca
     3181 gataggtgaa taactgcatg gggggagtca ttgcggccaa atcaaagctg gcgctgcctt
     3241 ccaggttttg ttgctgtcgc tgttcgatat agttgttgag atcctgagcg gtcactgcgc
     3301 tttccagacc ggcgtaagtc atcgcaaagg gtactatcag ggtcgccgcc actagcgtcg
     3361 caatcccaaa ccccaagcgg cgggccagag ggatattgcg tttgatgatc agcgaaccgg
     3421 caaaggcgat cacaatcatt cccgcgatgt ggggcctcac caacaacatg attgcgaccg
     3481 aagcgaccat gagccatacg cggcgcttca aattgagcgc cgcccataaa gcgaatccga
     3541 cagccatgaa tgacagggaa tctttgccga tggccgagga ccagaagctg actgacggca
     3601 aaaatacaat cagcgttgcc aggcgtcgaa cgtatttgga tttttcagca gtcgccgcct
     3661 gcaggctggc gtcgaaagcc agcaggccga tgaagccggc tatctgatag accagcgatg
     3721 cccctaaaaa agaaagcccc agaaccgaag tgaagaaaac tgtaatgaaa ctaacggcgg
     3781 ctgttcccac tgagaattca acgttcccag ccagcgctgt tttgtaataa tcaatcgcgt
     3841 cgccgccatt tgcgacgaga tacagggtgg cgataatgca aaaaatagtg tgccagccgt
     3901 agagtgtcag cgagcgtctg gccgtggtat taaagcgcct gccaacggaa atggccacga
     3961 gaatgcccgc tgcgaaaatc agtacgctgc ttaagaactg ccaggcggtt gaaaataatt
     4021 ccatggagcg gcgctattta aattttgatg gcccgcaggc cggggcgctc gaaaatggtc
     4081 ggttgcattt tgccgtatcg gcccatacgg cgtgatacga cgcgagcatt tttttcaagc
     4141 tgaaattttg cacaatgcgt tgccggcagt cgtcgcgccg tcgttgccag gtcagtggtt
     4201 gcagacgtct ttcgtctaac gccagggcga ttgcgttgga cagtgactgc gcatttcttg
     4261 caggaacgac ccagccggtg ttgccgacga tcaacgacgc gtcgcctacg ttggtggtca
     4321 cgcagggcgt ggcgcacgcc atggcttcgg ccagtacatt cggaaatgcc tctcccagac
     4381 tggacaaaac atgtacgtcc agagcgttca tgatggccgg tatgtcattg cgcgggccaa
     4441 gcaaaaacac atttttttcg accccagctt gctgcaacct gcgcatcaga tgatcattgc
     4501 ccggagtcat atcgcaaccc accagcaaac aagtgaaaac ccggtgttgg cggcgcagca
     4561 acatcagtgc ctcgattaaa ttctggtgat ccttttgtgg gtcaaatctg gcgaccatgc
     4621 cgatcagcgg tgcgttttcg tccagtttga attcttcgcg gattcgccgc cccgattccg
     4681 gacagggctg aagttcgccc agctcgtatc cgttcggtat gacgatgatt ttattggccc
     4741 ggtacccgat ttttttatgc aatggcgcgg catttaccga gcagcatacg atcctggcgg
     4801 gtatcgtgga agacagccac gcgcatagct tgatgatccg gggcgttgat ccggcgtttt
     4861 tgtcgcggtc aaaattggcg tgccgtattc cccagacgac agctttgacc ccggctaatc
     4921 gcgccgtgat gccgccgatc agatccgcgt ggtacatcca ggtttgcact acgtccggcc
     4981 tggcggattt aatgatgctg tatagcgtta acaagcctct caacgtaatt cggccgcgtg
     5041 gcatctgcag acaatggaca gtgcagtttg cctgctccag cttggcgccg taacggccac
     5101 ggtccataag ggaaatcact gtattttgat cgccgcattt ttggccggcc gccgcaagtc
     5161 tg
//
LOCUS       DATCXU010000463         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_366133,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000463 DATCXU010000000
VERSION     DATCXU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366133"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VIR04_10340"
     CDS             <1..299
                     /locus_tag="VIR04_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY9461189.1"
                     /translation="FALMVLMVRREWKGVATQRQRTRLYELVEFVGQWSMLDVFVVIL
                     LAAMANFPGLSQVTAGPGAVSFGVVVILTMFAAMSFDPRQGWDRPVAPHLRETP"
     gene            296..1951
                     /locus_tag="VIR04_10345"
     CDS             296..1951
                     /locus_tag="VIR04_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY9461190.1"
                     /translation="MTDQNTAPEPEDQTEPLAKPVVKPRRETRISWIWLVPLIAAAVG
                     LSLVVKTWLHTGPDVTISFESAEGIEPGQTKVRYRDVVVGVVTGIHVSSDRKKALVRA
                     ELDRDDAEFLTQEGARYWVVRPRLGATGVSGLGTLLSGVYIGVDAPESKEPGKSAYSF
                     VGLEKPPEVASGRPGTRFTLNAPDLGSLEIGSPVYYRRIQVGRVVGYDLNPNGSSVDI
                     QIFIDAPNDQFVTSDARFWNSSGINFSLNADGLTVQTGSLAAVMAGGISFISVHDGNT
                     TPAPRDTVFPLSRTQAQALADPDGPPFPIELRFRQSVRGLKVGAPVDFRGLELGKVID
                     IDLEFDTKANQFYALVKAELYPMRFGTVYEGLLKGAHNEVYPGQAVLAPLVKQGLRAQ
                     IRASNLLTGQQYVALDFFPDAEPVEFQKNKVPLELPTVAGSFDQLQQQIGRIVTKLDK
                     IPFDGISKDLRASLVSVTKLLDGLNSRVAPQATATLKAAQQSLHRVDKLLAQDSPLSD
                     NLDGTMRELTGAAKALRALADYLRATPSALVRGRAADVLPASP"
BASE COUNT          397 a          556 c          643 g          473 t
ORIGIN      
        1 tattcgcatt gatggtgctt atggtcaggc gcgaatggaa aggcgttgcg actcaacgcc
       61 agcgcacccg actgtatgaa cttgttgaat tcgtcggcca gtggtccatg ctagatgtgt
      121 tcgtggttat cttgttggct gccatggcga atttcccagg cctgtctcaa gtcacagcag
      181 gcccgggcgc ggtcagtttt ggcgtcgtcg ttattctgac tatgttcgcc gccatgagct
      241 ttgacccccg tcaggggtgg gataggcctg tcgcgccgca tttgcgggaa acgccatgac
      301 cgaccaaaat actgcgccag agcccgaaga tcagacggag ccattggcga aacccgtcgt
      361 caagcccagg cgtgaaacca gaatctcctg gatctggctg gttcccctga tcgcggctgc
      421 ggtcggattg tcgctggtcg tcaagacgtg gctgcatacc ggaccggatg tcacaatcag
      481 ttttgaatcg gccgaaggca tagagccggg gcagaccaag gtgcgttacc gcgacgtggt
      541 ggtgggtgtg gtgacgggaa ttcacgtttc ctctgatcgc aaaaaggctt tggtacgagc
      601 cgagctggat cgtgatgatg ctgaattcct gacccaggaa ggcgcacgct actgggtggt
      661 gcgcccgcgc ctgggagcca ctggcgtgtc gggtctgggg accttgctgt cgggtgtcta
      721 tattggtgtc gatgcgcccg aaagcaaaga gccggggaag tcggcatata gttttgttgg
      781 tctggaaaag ccgcccgaag tggccagtgg ccggccgggc acgcgtttta ccttgaacgc
      841 gcctgatttg ggctcccttg aaattggctc ccctgtttat taccggcgta ttcaggttgg
      901 tcgcgtggtc gggtacgact tgaatcctaa tgggagctcg gtcgatatac agatattcat
      961 cgacgccccc aacgatcagt tcgttacctc ggatgcccga ttttggaatt ccagcggtat
     1021 aaatttttcg ttaaacgccg atggcttgac agtgcagacg gggtcgctgg ccgcggtgat
     1081 ggcgggcggc atatcgttca tttccgtgca tgatggcaac accacgcccg ctcctcgcga
     1141 tacggtcttt cctctcagcc gcacgcaggc gcaggccctg gccgaccctg atgggccgcc
     1201 ttttcccatc gagctgcgtt ttcgacaatc ggtccgtggc ctgaaggtgg gcgcgccggt
     1261 cgattttcgc ggcctggagt taggcaaagt gattgatata gatctcgagt tcgataccaa
     1321 ggcgaatcaa ttttatgcat tggtcaaagc ggaactatat cccatgcgct ttggcacggt
     1381 gtatgaaggt ctgctcaagg gtgcccataa cgaagtctat ccgggtcagg ctgttttggc
     1441 tccgttggtc aagcaaggct tgcgtgctca aatacgtgcg tccaatttgc tgacgggcca
     1501 gcaatatgtc gcgctggatt ttttcccgga tgccgaaccg gttgaattcc agaagaataa
     1561 ggtgccgctg gagttgccaa cggttgcggg cagcttcgat cagttgcagc agcaaatcgg
     1621 caggattgtc accaaactgg acaagattcc attcgacggc atcagcaagg atttgcgcgc
     1681 cagcctggtg tcggtgacga agctgctgga tggattgaac tccagggtgg cgccgcaggc
     1741 aaccgcaacg ctgaaagccg cgcagcagtc tttgcaccgg gttgacaaac tgctggcgca
     1801 ggattcgccc ttaagcgata accttgacgg gacgatgcgc gaactgactg gcgcggccaa
     1861 ggcgttacga gccttggcgg actatttgcg cgccaccccc agtgccctgg ttcgcggccg
     1921 cgccgctgat gtgttgcccg ccagccctta ggaatcaagc atgaaaatct tgcaatggat
     1981 cagcgctatg gcggtgattg cgagtgtggc gggttgcgcc gccccgccgg ccccgcagta
     2041 ttacactttg ttgcccagca ccgggccgg
//
LOCUS       DATCXU010000464         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_366159,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000464 DATCXU010000000
VERSION     DATCXU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366159"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VIR04_10350"
     CDS             complement(<1..477)
                     /locus_tag="VIR04_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9461191.1"
                     /translation="MSPALLTVSGLTMRFGGLLAVDGVDFEVMRNEVFAIIGPNGAGK
                     TTVFNCVGGFYKPTAGSVVMDGKKINGFPSHKVARHGLVRTFQNVRLFKQLTVLENLL
                     VAQHTHIQTGLLQGLFKVPSFRKSELEARRNAAWWLDFMGIRQFANREAGNLAYGHQ"
     gene            complement(474..1703)
                     /locus_tag="VIR04_10355"
     CDS             complement(474..1703)
                     /locus_tag="VIR04_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEY9461192.1"
                     /translation="MGRNLFAAIVAALMAGVIIAPVYGLRIVQQGMQTHLRPEWTMVF
                     TGMGIVFVVQFLRPWLKSRFIGKVKKLPALPVLSDKARGRVILFLVLVALVWPFFGGR
                     AQVDIATLVLIYVMLGLGLNIVVGFAGLLDLGFVGFYAVGAYTYALLYHWAGWGFWQA
                     LPLAGGMSALFGFVLGFPVLRLRGDYLAIVTLGFGEIIRLMLTNLYSLTGGPDGISGI
                     PKPTVFGYPMVRRAAEGQTTFHQLVGWKFDNQDVVIYLYLMALCLALFALFVSTRLIR
                     MPIGRAWEALREDEIACRSLGLNPTRIKLSAFTMGAMFAGFGGAFFAARQGLVNPESF
                     TFIESALILSIVVLGGMGSQVGVILAAIILTVTPELAREFAQYRMLVFGMVLVIMMVW
                     RPQGLLPVKRPQVELTP"
     gene            complement(1703..2629)
                     /locus_tag="VIR04_10360"
     CDS             complement(1703..2629)
                     /locus_tag="VIR04_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249233.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEY9461193.1"
                     /translation="MSALLPQLTQQFFNGLSLGAIYGLIAIGYTMVYGIIGMINFAHG
                     EIYMLGSYVGLVTLSAIGVNSGLPLPLIILVMILVAVAVTGVYGYAVEKIAYAPVRSS
                     PRLVPLISAIGMSIFLQNWVAIGQGARDMAVPALVTGSVQFTMGSDFEVTAPYSRIII
                     IVVTIILMAGLSLFIKYSRTGQASRACSQDLAMANLLGIDTSRIISFTFVVGAMLAAV
                     GGVLIALSIGKLNPFIGFIAGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFSAAYIS
                     SQYKDIVAFLLLVFILMFRPSGLLGKPEVEKV"
     gene            complement(2698..3270)
                     /locus_tag="VIR04_10365"
     CDS             complement(2698..3270)
                     /locus_tag="VIR04_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEY9461194.1"
                     /translation="MKRHQPFRFVPMNPAQFLLAVAAMGVIVVGSNVLVQYPINHWLT
                     WGGVSYPVAFLVADVLNRRFGPGAARKVAFVGFVIALAISAWAATPRIALASGLAFLS
                     AQLIDIHVFDRLRDRRWWRAPLLGGVAGAVLDTSIFFSVAFAGTGVHWMALLLGDLAV
                     KLAVNLTMLAPFRAMMWNLARPAALQRDTL"
     gene            complement(3267..4100)
                     /gene="proC"
                     /locus_tag="VIR04_10370"
     CDS             complement(3267..4100)
                     /gene="proC"
                     /locus_tag="VIR04_10370"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY9461195.1"
                     /translation="MDKEFSISLIGGGNMATALASGLMGKPCSAHKVHVIDPNASVLA
                     RWKEQGATVAAAPDDTLAGHGVWIFAVKPQHLKESALQARPYLRPDTLVISIAAGIPS
                     TTLAGWLGTPNQPWTRLVRCMPNTPALIGCGITGMIAMQGASDYDRSVAWQLMHAVGD
                     AIWFDNDQQLDAVTALSGSGPAYVFLFIEALMQGGKKLGLSEDQSRALALATLSGATQ
                     LAATSHEPPATLRERVTSKGGTTAAALAVFGQKEFALIIEQAMRAAYDRAAALASELS
                     Q"
     gene            4257..>4610
                     /locus_tag="VIR04_10375"
     CDS             4257..>4610
                     /locus_tag="VIR04_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249229.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY9461196.1"
                     /translation="MRAKYFALSLAGLIATLGTAHAATDINFWHSMQGPLGDRVNSIV
                     EQFNKSQTDYTVHAIYKGTYGESMNAGIAAFRGGNAPDILQVFEVGPATMMYAKGAIV
                     PVQEMSEKAGDPINPK"
BASE COUNT         1190 a         1388 c         1204 g          828 t
ORIGIN      
        1 ttggtggcca tacgcaagat tgccggcctc acgattggca aattgacgaa tccccatgaa
       61 atcaagccac cacgctgcat tgcgccgggc ttcgagttcc gatttacgaa aagacggaac
      121 cttgaacaag ccttgcaaca ggcccgtctg gatgtgcgtg tgctgcgcaa ccagcaaatt
      181 ttccagtacg gtaagctgct tgaaaagccg cacgttttga aatgtgcgca caagaccatg
      241 gcgtgcgact ttgtggctgg gaaatccgtt gatttttttg ccgtccatga cgacactgcc
      301 cgctgttggc ttgtaaaagc ccccaacaca attaaatacc gtcgtcttgc cggcaccatt
      361 gggcccgatg atcgcgaaga cctcgttgcg cataacctcg aagtccacgc catcgacggc
      421 cagcaaaccg ccaaatcgca tcgtcaagcc cgatactgtc aacagtgccg ggctcatggc
      481 gtcagttcaa cctgagggcg cttgaccggc agcaggcctt gcggtcgcca taccatcatg
      541 atcaccagca ccataccgaa aaccagcatg cggtattgcg cgaactcgcg cgcaagctcc
      601 ggcgtgaccg tcaggatgat tgcggccaaa attacgccca cttgagatcc catccctccc
      661 agaaccacaa tagacaaaat cagggcagac tctatgaacg tgaacgattc gggattaacc
      721 agtccctggc gcgcggcgaa gaaagcgccg ccaaagcctg cgaacatggc gcccatcgtg
      781 aacgcggaca gcttgattcg agttggattc aagcccaaag aacgacaggc tatctcgtct
      841 tcgcgcaaag cttcccacgc gcggccaata ggcatgcgta tcagacgggt cgacacaaac
      901 aaagcaaaca aagccaagca taacgccatc aggtacaaat aaatgacgac atcctgattg
      961 tcaaatttcc agccgaccaa ctggtgaaat gtggtctggc cttcggccgc ccgacgtacc
     1021 atgggataac caaacaccgt cggtttcggt atgccggaaa taccatcggg gccgcctgta
     1081 agcgaataca agttcgtcag cataagacga attatttcac caaatccaag cgttacaatc
     1141 gccaggtaat cgccgcgcag gcgcaatacc gggaacccca aaacaaaccc gaacagggcc
     1201 gacatgccgc cggcaagcgg caaggcctgc cagaaccccc agcccgccca gtgataaagc
     1261 aaggcgtagg tatacgcccc cacggcatag aaaccgacga acccaagatc cagcaatccg
     1321 gcaaagccca ctacaatatt caggcccagg cccaacataa cgtagatcag cacaagtgtc
     1381 gcaatatcga cttgcgcccg gccgccaaaa aatggccata caagcgcgac gagcaccagg
     1441 aataaaataa ctcggccacg ggctttgtcg gacagcactg gcaacgcggg aagctttttg
     1501 actttgccga tgaatcgact tttcagccag gggcgcaaaa actgaactac gaacacaatg
     1561 cccatgccgg tgaaaaccat ggtccattcg ggcctgaggt gggtttgcat gccttgttga
     1621 acaatccgca aaccatagac gggcgcaata atcacgcccg ccatcagcgc ggccacgatg
     1681 gcggcgaata aattcctgcc cattacactt tttccacctc aggcttgccc agtaacccgc
     1741 tggggcgaaa catcaggata aaaaccagaa gtaaaaaagc gacaatgtct ttgtactgag
     1801 acgagatata ggcggcggaa aatgtttcgg ccagtcccag caaaacaccc ccaagcatcg
     1861 ctccgggaat gcttccaatt ccgccgagaa cggcggcggt aaacgctttg atgcccgcta
     1921 taaaacctat gaacggatta agttttccga tcgacagcgc gatcaggacg ccgccgaccg
     1981 ccgccagcat ggcgccgaca acaaacgtga aggaaatgat gcggcttgtg tcaataccaa
     2041 gcaggttggc catcgcaaga tcttgagaac aggcgcgcga cgcctggccg gtgcgcgaat
     2101 atttgataaa aagcgacagc cctgccatga gtattatcgt tacaacaata atgatgattc
     2161 tggaatacgg cgcggtaact tcaaaatccg accccatcgt aaattgcacc gagcctgtaa
     2221 ccagcgcggg aacggccatg tcgcgcgcgc cttgcccgat cgcaacccag ttttgcagga
     2281 aaatcgacat cccgatagcg gaaataaggg gaacaaggcg cggactgctg cgtacagggg
     2341 cataggcaat cttttccacc gcgtagccgt atacgccggt cacggccaca gcaaccagaa
     2401 tcatgacgag aataatcagc ggcaacggca agccactgtt cacgccgatg gcggacaagg
     2461 tcaccagacc cacgtaactg cccagcatgt aaatctcgcc gtgcgcaaaa ttaatcatgc
     2521 cgataatgcc atagaccatg gtgtagccga tggcgataag tccataaatc gctcccagcg
     2581 acaagccgtt gaaaaactgc tgcgtgagct gaggcaataa tgcggacatg aaaacggctg
     2641 ttccggtggg tcaggactcg acatgccgcc caaggcggcg tcccgatgta aaaatttcta
     2701 aagtgtgtcg cgttgcagcg cggcaggccg cgccaaattc cacatcatgg ctctgaaggg
     2761 agccagcatg gtgagattga cggccaattt gaccgccaga tcgcccagca gcagagccat
     2821 ccaatgcacg cccgttcccg cgaaagcaac gctgaaaaag atggacgtgt ccagaaccgc
     2881 gccggccaca ccgcccagca ggggtgcgcg ccaccagcgg cgatcgcgca gccggtcgaa
     2941 aacatggata tcgatcaatt gcgcgcttaa aaacgcaagc cctgacgcca gcgcaatgcg
     3001 cggcgttgcc gcccatgccg aaatcgccag cgcaatcaca aagccgacaa aggccacctt
     3061 gcgagcggcg cccggaccga agcgccgatt caacacatcg gcgaccagaa aagccacggg
     3121 atatgagaca ccgccccatg tcagccaatg gttgatggga tattgcacca atacatttga
     3181 gccgaccaca atcacgccca ttgcggccac ggcgagcagg aactgcgccg gattcatcgg
     3241 tacaaaccgg aacggctggt gtcgtttcat tgggagagct cgctggcaag cgccgccgcg
     3301 cggtcgtaag cggcgcgcat ggcctgctca atgatgagag caaattcctt ttgcccgaaa
     3361 acagccaatg ccgccgcggt cgtcccgccc ttagaggtaa cccgttcacg cagagtcgcc
     3421 ggaggttcgt gcgatgtcgc cgccaactga gtggccccgc ttaatgtagc cagcgccaga
     3481 gcccgtgatt gatcctcgct caagcccagc ttcttcccgc cttgcatcag cgcttctatg
     3541 aataaaaaca cataggccgg cccgctaccc gataaagccg tgaccgcgtc aagctgctga
     3601 tcgttgtcaa accagatggc gtcgcccacg gcatgcatca attgccaggc aacgcttctg
     3661 tcgtaatcgg acgcgccctg catcgcaatc attcccgtga ttccgcagcc tatcagcgcg
     3721 ggagtattgg gcatgcaacg caccaggcga gtccagggct ggttcggcgt gccgagccag
     3781 cccgccaggg tcgtgctggg aattcccgct gcaatactga tcaccagcgt atcggggcgc
     3841 aaataaggtc tggcctgaag ggcactctcc ttcaggtgct gcggcttgac cgcaaaaatc
     3901 cagacgccgt ggccggccag agtatcgtca ggcgcggcgg caaccgttgc gccctgttct
     3961 ttccagcgag ccagcaccga cgcattgggg tcgataacgt gaactttatg ggccgagcac
     4021 ggtttgccca tcagaccgga cgccaaggcc gtagccatat tgccgccgcc gattaatgaa
     4081 atgctgaatt ctttatccat ggctgcgtat tgtacgattt tccacgccgc gggtatcgac
     4141 accgtattag tacatctttg cattgacctg ccgcccaaat agtcgttaca ttcataccgc
     4201 tgtagcaagc gcgcctctga actggcaggg gcggaacgtc tctaggagaa aagctcatgc
     4261 gagcaaaata ttttgccctg tccctggctg gattaatcgc aacgctcggt acagcgcatg
     4321 cagcgacgga catcaatttc tggcactcaa tgcaaggccc ccttggcgat cgcgtcaact
     4381 ccatcgtcga gcaattcaac aaaagccaaa ccgactacac cgttcatgct atttacaaag
     4441 gaacctacgg cgagtcgatg aatgccggca ttgccgcatt ccgcggcgga aacgcgcctg
     4501 acatcctgca agtattcgag gttggacccg ccaccatgat gtacgccaag ggagctatcg
     4561 tacccgttca ggaaatgtcc gaaaaggccg gcgaccctat caatcccaag
//
LOCUS       DATCXU010000465         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_916442,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000465 DATCXU010000000
VERSION     DATCXU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916442"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1156)
                     /locus_tag="VIR04_10380"
     CDS             complement(65..1156)
                     /locus_tag="VIR04_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY9461197.1"
                     /translation="MPAQIDTSACPNPVWLPGRDWQTVYGALFAGYHRIAFVRDRVNT
                     PDGDFLDFDWIGPGLFANMTAAGSKASPDIRLVQTAARRWAQDEDWDALPNIPDTPAL
                     VLFHGLEGSSKSHYAQAIAQYFRARGWIVAIAHFRGCSGFRNRMARAYHSGDSDDVSF
                     IFDTVRKRAPHAKWYAVGVSLGGNAMLKYLGENTEIAQWLSASAAVSAPTDLVACGNR
                     LSDSWIGRQLYTRYFLKAMKEKVFEKARRFPGTIDVLKLNQVRTLRQFDNLYTAPMHG
                     YKNALDYWTRASSKPHLKALTVPTMVLNARNDPFIPEPSLPGSLDCSDQVLLHQPAEG
                     GHCAFVTGPFPGHLNWLPTRLARFFETGR"
     gene            complement(1166..1588)
                     /locus_tag="VIR04_10385"
     CDS             complement(1166..1588)
                     /locus_tag="VIR04_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY9461198.1"
                     /translation="MFATPDDAEYAFYDAIRRSDLTRMMLVWADDDEIVCVRPDGARS
                     IGQDAIKTAWQQIFSNGPMAIYPARPLVLGSIASSIHILMEQVAIRTPFGTETANCYT
                     TNIFLKGPLGWCMVLHHASSAPNDAGLFDPQDIPDTLH"
     gene            complement(1593..1823)
                     /locus_tag="VIR04_10390"
     CDS             complement(1593..1823)
                     /locus_tag="VIR04_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY9461199.1"
                     /translation="MSAVEQPSESQDVKIPKDQTVFVHAKDLPVYCPGPHSPLWSMHP
                     RVYIEVVKTGRAKCPYCGTAYQLSPGEKIQEH"
     gene            complement(1870..2793)
                     /locus_tag="VIR04_10395"
     CDS             complement(1870..2793)
                     /locus_tag="VIR04_10395"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY9461200.1"
                     /translation="MASMADRDGFIWYDGELVPWRNATTHVLTHSLHYGLAVFEGVRA
                     YKTDIGTAIFRLHEHINRLFNSAHIYQMKIPYDRETISAAHMEVVRRNELESCYIRPL
                     VFYGSEKMGVSPRGATVHVAIAAWPWGAYLGEEALKKGIRVKISSFARQNVNVTMPRA
                     KVASTYANSILANAEALDNGYDEALLLDTDGFIAEGSGENVFIVKDGVLCEPEIASAL
                     TGITRSTIHSLAADLGIPLQTRRLTRDDVYIADEVFFTGTAAEVTPIREVDKRVIGAG
                     ERGPVTEKLQKAFFDVVNGRNPKYLSWLSKV"
     gene            2978..>4238
                     /locus_tag="VIR04_10400"
     CDS             2978..>4238
                     /locus_tag="VIR04_10400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY9461201.1"
                     /translation="MTESTISATPITTFSDFGLHPSILLAIAATGYTTPTPIQAQSIP
                     LIMDGRDVMGAAQTGTGKTAAYTLPILHRLMPLASHSASPARHPVRVLILAPTRELAD
                     QVYDNVVQYCKPTPLRATVVFGGVDIGPQRDALRHGCEILVATPGRLLDHIEQKNVNL
                     SHVSILVLDEADRMLDMGFLPDLDRIVRLLPPGRQGLLFSATFSNEIRKLGRTYLKNP
                     AEIDVAARNATADNVTQIAYPLSGSEKRAAVVHLVRSRGFNQVIVFSNTKIGTSRLAH
                     QLERDGIKAESIHGDKSQHDRMKALEAFKAGELEVLVATDVAARGLDVAGVPCVINYD
                     LPYNAEDYIHRIGRTGRAGASGEAIAFYAPDEERYLLDIEKLIKYKIPRGHLGLPAAS
                     ASTSARMVHSNGRARSEHRNGYANASKA"
BASE COUNT          922 a         1147 c         1233 g          936 t
ORIGIN      
        1 tagtcggagc tgcgagggcg gggcagactc gcgccgatgg cgctcaaact gcggtggtca
       61 aatttcagcg tccagtttcg aaaaaacgag ccagccgggt cgggagccag ttcaggtggc
      121 ccgggaaagg gccagtaacg aacgcgcagt gccccccttc ggcgggttga tgcaagagaa
      181 cctggtccga acaatccagc gatcccggca aggatggttc agggatgaat gggtcgtttc
      241 gcgcattaag caccatcgtg ggaacggtca gtgcttttaa gtgtggcttg ctcgacgcgc
      301 gcgtccagta gtcaagggcg tttttatagc cgtgcatggg cgccgtgtac agattgtcga
      361 attggcgcag tgtgcgaacc tgattaagct tcagtacatc gattgttccc ggaaagcgcc
      421 gggctttttc gaaaaccttt tctttcatgg ccttcaaaaa atagcgggta tagagctggc
      481 ggccgatcca actgtcggaa aggcggtttc cgcaagcgac cagatcagtc ggcgccgaga
      541 cagcggcgct ggcggaaagc cactgcgcga tttctgtgtt ttctcccaag tatttgagca
      601 tggcgttgcc gcccagcgat acgccaacgg cgtaccactt ggcgtgcgga gcgcgtttgc
      661 gcacagtatc gaaaatgaag ctgacatcgt ccgaatcgcc agaatggtaa gcgcgagcca
      721 ttcgattcct gaatcccgag caaccgcgaa aatgagcaat tgcaacaatc cagccgcgtg
      781 cgcgaaagta ttgagcaatc gcctgggcat aatggctttt gctgcttcct tccaaaccgt
      841 ggaagagcac cagcgcgggt gtgtccggga tattcggcaa ggcatcccag tcttcatcct
      901 gggcccatcg acgcgcggct gtttggacca gcctgatgtc ggggctggct ttggaacccg
      961 ccgccgtcat gttggcaaat aagccaggtc ctatccagtc aaaatcaagg aagtcgccgt
     1021 ctggcgtgtt gacgcgatcg cgcacgaacg cgatgcgatg gtatcccgcg aacagggcgc
     1081 catataccgt ttgccaatcg cgtcccggca accagacggg attggggcag gcggaagtat
     1141 caatttgcgc tggcaagaat tgccctcagt gcagggtgtc gggaatgtct tgcgggtcga
     1201 acaaacctgc gtcgttgggc gcgctggagg cgtgatgcag aaccatgcac cacccgagcg
     1261 ggcccttgag aaagatattg gttgtgtagc aattagccgt ttctgtgccg aacggagtcc
     1321 gaatggcgac ttgttccata agaatatgga tcgaacttgc gatgctgccc aagaccaatg
     1381 ggcgagccgg gtaaatcgcc atggggccat tggaaaaaat ttgttgccac gcggttttta
     1441 tagcgtcttg tccgatcgat cgcgcgccgt caggacgtac gcacacaatt tcgtcatcgt
     1501 cagcccaaac cagcatcatg cgggtcaggt cggaccggcg aattgcatcg tagaaagcgt
     1561 actccgcgtc gtctggggtg gcgaacatgg gttcagtgtt cttggatttt ttcgcccgga
     1621 ctaagttgat aagccgtgcc gcagtagggg catttggccc gaccggtttt gacgacttcg
     1681 atgtatacgc gcggatgcat gctccaaagc ggagaatgcg gccccggaca atacaccggc
     1741 aagtctttgg cgtggacgaa tacggtctgg tcttttggaa tcttcacatc ctgagattca
     1801 gaaggctgtt ctactgcact catggtgttt cctggaaatg atcgaataac ggtcggaagg
     1861 gagtcgaagt cataccttgg agagccagct gaggtatttc gggttgcggc cgttgaccac
     1921 gtcgaaaaac gccttttgca gtttttctgt gacggggccg cgttcgcccg ccccaatgac
     1981 tcgtttgtcg acctcacgga taggggtgac ttcggccgcc gtgcctgtaa agaaaacttc
     2041 gtcggcaata tagacgtcat cgcgggtcag gcgtctggtc tgaaggggaa tgcctaagtc
     2101 ggcggccaga gaatggatcg tcgaacgtgt gatgcccgta agtgccgagg cgatttcagg
     2161 ttcgcacaga accccgtctt taacgatgaa gacgttctcg ccggaacctt ccgcaatgaa
     2221 gccgtcggta tcgagcagga gcgcttcgtc gtagccatta tcgagcgctt cggcgttagc
     2281 caggatcgag ttggcgtagg tgctggccac cttggcgcgc ggcatggtga cattgacgtt
     2341 ttgacgcgcg aacgaggaaa tcttcacgcg aatgcctttt ttcagggctt cttcacccaa
     2401 atacgctccc cacggccaag cggcaatcgc cacatgaacc gttgcccctc tgggtgatac
     2461 gcccattttt tccgaaccgt aaaacaccaa tgggcggatg tagcaggact caagttcgtt
     2521 gcggcggacc acttccatgt gcgcggcgct aatggtttcc cgatcgtagg gaattttcat
     2581 ctggtagatg tgggcggaat tgaacaggcg gtttatgtgt tcatgaagcc ggaaaatcgc
     2641 ggtgccgatg tcggttttat aggcgcgcac gccttcgaag acggcaagac cgtaatgcaa
     2701 agagtgggtc agcacatggg ttgtggcgtt gcgccacggc actaattcac cgtcgtacca
     2761 aataaaacca tcgcgatccg ccattgacgc catattgctc tcctaagatg aaatgttatt
     2821 gtagcaagta ggagacacaa aattaccgtg gacatttaac gacacttgac gcagtcaaaa
     2881 gcggcataaa ttggggactt ccgcaagctt gcgttaaaat tatccggttc caacagccat
     2941 gtgcgggttt tcccgtattg ggcgcttact atattaaatg actgaatcca ctatttccgc
     3001 tacacccatc accacatttt ccgattttgg cttgcaccca agcatcttgt tggccatcgc
     3061 ggcaaccggt tacaccaccc ccactcccat tcaggctcaa tccataccgc tcattatgga
     3121 cggccgcgac gtcatggggg ctgcccagac gggaaccggt aaaacagccg cttacacttt
     3181 gccgatcctg catcgcctga tgccgcttgc cagccatagc gcttcgcctg cccgccatcc
     3241 tgttcgtgtg ctcatccttg caccgacccg cgaactcgcg gatcaggtct atgacaacgt
     3301 tgtgcagtat tgcaaaccga ccccattgcg cgcgacggtt gtgtttggcg gcgtcgacat
     3361 tggcccgcaa cgcgacgcac tgcgccacgg ttgcgagatt ctggtcgcga ctcccggacg
     3421 tttgctggat catatcgaac aaaaaaatgt gaatctgagc cacgtaagca tattggtgct
     3481 cgatgaggct gatcgcatgc tcgacatggg tttcctgccg gacctggatc ggattgtgcg
     3541 tttattgccc cctggccgcc agggactgct tttttcggcg acattcagca acgaaatccg
     3601 caagttgggc cgcacgtatc tcaagaatcc cgccgagatc gacgtggccg cgcgcaatgc
     3661 gacggctgac aacgtgacgc agattgccta cccgttatcg ggcagtgaaa agcgcgctgc
     3721 cgtcgtgcat ctggtccggt cgcgcgggtt caatcaagta attgtgtttt ccaatacgaa
     3781 gatcggcacc agccgtttgg cgcatcaact cgagcgcgat ggaatcaagg ccgaatccat
     3841 tcacggagac aagagtcaac atgatcgcat gaaggcgctc gaggcgttca aggcgggcga
     3901 gctcgaggtt ctggtggcga ccgacgttgc tgcgcgcgga ctcgatgtgg cgggtgtgcc
     3961 ctgtgtcatt aactacgatc tgccgtataa cgccgaagat tacattcatc gtatcggccg
     4021 cacgggccgg gccggagcgt cgggcgaagc tatcgccttt tatgcccccg acgaagagcg
     4081 ctatctgctg gatattgaaa agcttatcaa atacaagatt ccacgcggtc acttggggtt
     4141 gccggcggca agcgcgagca cgagcgcacg catggttcat tccaatggcc gcgctcgttc
     4201 cgagcaccgc aatggctacg caaacgcttc gaaggcca
//
LOCUS       DATCXU010000466         5776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_366384,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000466 DATCXU010000000
VERSION     DATCXU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366384"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VIR04_10405"
     CDS             complement(<1..938)
                     /locus_tag="VIR04_10405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY9461202.1"
                     /translation="MPQPTEDHQAIRDAIRALCAQFPSEYFRKLDEQRSYPEEFVDTL
                     TKAGWLAALIPEEYGGSGLSLTEASVIMEEINRAGGNSGACHGQMYNMGTLLRHGSKE
                     QKEHYLPRIASGELRLQSMGVTEPTTGTDTTKIKTMAVKKGDRYVVNGQKVWISRIQH
                     SDLMILLARTTPLDQVKKKSEGMSIFIVDLHQAIGHGLQVRPIPNMVNHETNELFFDQ
                     LEIPVENLIGQEGMGFKYILDGLNAERTLIAAECIGDGYWFIDKVSAYAKERIVFNRP
                     IGQNQGVQFPIARALVNVEAASLMRYEACRLFDAHQA"
     gene            1052..1999
                     /locus_tag="VIR04_10410"
     CDS             1052..1999
                     /locus_tag="VIR04_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461203.1"
                     /translation="MAMRFDLIDMQLMVNVASARSMTKGAERSFLSLPAASTRVKNLE
                     SHLGTALLYRSSQGVTLTPSGEAFVRHARVVLRQLEHLRGDIQEYANGVKGRIRIVAN
                     TTAMNEFMPEVLGRYLSSHPDVNVELRERLSFLVVKAIVEGSADVGVTALAEGAQDIE
                     FLPYRTDRLVLVTPLDHPLAVADTVDFDETLGFDYVGLSEASAIHAFLLQAADDLGRV
                     LRFRVEVSNFEAACRMISAHVGIGVIPESAAQRYARNLPLKLIALNDEWALRKLHICV
                     RRLDSLPVFAQEFVSLLVKDAAQTDACVGARVNQLMPGK"
     gene            1996..2628
                     /gene="rdgB"
                     /locus_tag="VIR04_10415"
     CDS             1996..2628
                     /gene="rdgB"
                     /locus_tag="VIR04_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY9461204.1"
                     /translation="MNSSTRSTPGRRIVLASNNAGKLKEFSAILQSAGVVMVAQGDLG
                     VPEAAEPFGTFVENALAKARHASVITGLPALADDSGLCVNSLNGAPGVFSARFSALHG
                     GEKSDEANNQCLLRLLRTQNNRSASYIAVLVYVRSAADPCPIIAEGAWAGEIIDEPRG
                     LHGFGYDPYFYLPKLGKTAAELTPRLKNRLSHRARALKKLLQALSTHNEI"
     gene            2644..3831
                     /gene="hemW"
                     /locus_tag="VIR04_10420"
     CDS             2644..3831
                     /gene="hemW"
                     /locus_tag="VIR04_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY9461205.1"
                     /translation="MPRVPDKPAARPTTLAGLPPLSLYVHVPWCVHKCPYCDFNSHEL
                     KGELPERDYLAALRADLEQALPLVWGRRVVSIFIGGGTPSLLSASAIDQMMTLFRSCL
                     NVLPDAEVTLEANPGVAEAARFMEYANSGVNRISLGVQSFNDQSLSALGRIHDSNQAR
                     KAIGFAQRAVDRVNLDIMYALPRQALEGCADDLRTAMSFRTEHLSLYHLTFEPNTVFA
                     KYPPTVADDDTSAAMQELIAEQTSLNGWERYEVSAYAQPGARCRHNLNYWEFGDYLGI
                     GPGAHGKLSFHDRIIRQSKLKNPASWMDKAQRMDNSHIAEERVVGADELGFEFMLNAL
                     RLKSGVSAASFTQRTGLALHSIRAPLDRAIARGLLESDLARLRATPLGWRFLNDLQME
                     FLE"
     gene            4022..5434
                     /gene="glnA"
                     /locus_tag="VIR04_10425"
     CDS             4022..5434
                     /gene="glnA"
                     /locus_tag="VIR04_10425"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY9461206.1"
                     /translation="MSSPEDVVKLIAEREVAFVDFRFTDTLGREHHMTVPGHTVDEEK
                     LESGQAFDGSSLPGWKGIEASDMVLVPDPNTAHMDPFREETTLILSCDVVEPSDLKGY
                     DRDPRSLAKRAEAYLKSSGLGDTAYFGPEPEFFIFDGVAWNDDMSGCFVKVKSDEAPW
                     SRSIDPDNGNLGHRPNAKGGYVPVPPVDSFQDIRSEICLLLEQQGVPVEVHHHEVAAP
                     GQLEIGTKFSTLVQRADWNQILKYTIHNVAYVYGKTATFMPKPIVGDNGSGMHVHQSI
                     WKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPHFEAPVKL
                     AYSARNRSASIRIPYVNSPKARRVETRFPDPLANPYLAFSALMMAGLDGVQNKIHPGD
                     PADKNLYDLPPEEDAKIPTVCASLDEAIAALDSDREFLTRGGVFSNDMLDAYIELKMA
                     EITRLRMTTHPVEFDMYYSL"
     gene            5468..>5776
                     /locus_tag="VIR04_10430"
     CDS             5468..>5776
                     /locus_tag="VIR04_10430"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY9461207.1"
                     /translation="MLASTILLLNNQGEVEYANAAAQELLGLPRKQLTGLFGYSLFKA
                     DTALESRFLDAINGKFGILRQDLFVDRLGSTLPVSLTIVPLADRPWAALLEVRAVEQH
                     V"
BASE COUNT         1215 a         1516 c         1623 g         1422 t
ORIGIN      
        1 gcctgatgcg cgtcgaacag acggcacgct tcgtaacgca tcaggctggc cgcttcgacg
       61 ttgaccagtg cgcgcgcgat ggggaattgc accccctggt tctgtccgat gggacgatta
      121 aatacgattc gttcctttgc gtaagccgag actttatcga taaaccaata accgtctcca
      181 atgcattcgg cggcgatcag cgtgcgctcg gcgttcaggc catccagaat atatttaaaa
      241 cccatgcctt cctgaccgat aagattttcg accgggattt caagctggtc gaaaaacagc
      301 tcgttggttt cgtgattgac catgtttgga ataggacgca cttgcagacc gtggccgatt
      361 gcctggtgta aatcgacgat gaagatcgac atgccttcgg attttttctt gacttgatcc
      421 agtggcgtag tgcgggccag cagaatcatc aaatcggaat gctggatacg cgatatccag
      481 actttttgcc cgttcaccac atagcgatcg cccttcttga ctgccatcgt tttaatcttt
      541 gtagtgtccg tgccggttgt gggctcggtc acccccattg attgcagacg caattcgccg
      601 ctggcaatgc gcggcaaata gtgttctttt tgttctttgg agccatgacg caatagtgta
      661 cccatgttgt acatttggcc gtggcaagcg ccggaatttc cacccgcgcg gttgatttcc
      721 tccataatga ccgaggcttc ggtcaggcta aggcccgaac cgccgtattc ttcgggaatc
      781 agggccgcca gccagccggc tttggtcagt gtgtcaacga attcctcggg gtagcttctt
      841 tgttcgtcta gctttctaaa gtattcggag gggaattggg cgcataaagc gcgaattgcg
      901 tcgcgaatgg cttggtggtc ttcggttggc tgcggcataa ggggctatct tgaattcatt
      961 acaatgtttg cactgtgcac gaatacttga gttttgccaa ttatcgcttg attaagcggg
     1021 ggtttgtcaa ttttgaagag aacacaattc gatggcaatg cgctttgact tgatcgatat
     1081 gcagcttatg gtcaatgtgg ccagtgcgcg cagcatgacc aagggagctg aacgttcttt
     1141 tttgtctttg ccggccgcca gtacgcgcgt caagaatctt gaaagtcatt tgggcaccgc
     1201 actgttgtat cggtccagcc agggggttac tttgactccg tcgggagaag cttttgtccg
     1261 ccacgcgcgc gtagtattgc gccaactcga acatttgcgc ggcgatatcc aggaatatgc
     1321 aaacggcgtc aagggccgta tcaggatagt ggccaatacg acggccatga atgaatttat
     1381 gcctgaagtg cttggacgct atctgtccag ccatcccgat gtcaatgtgg aattgcgcga
     1441 acggctcagt tttctggtgg tcaaggcaat tgtggaaggg tcggccgatg tcggcgtgac
     1501 ggccttggca gagggtgcgc aggacattga gttcttgcca tatcggacgg accgtttagt
     1561 gttggtcacg ccgctcgatc atcctctggc ggttgccgac actgtggatt tcgacgaaac
     1621 actgggtttc gactacgttg gtttatccga ggccagtgcg atccatgcct ttttgctgca
     1681 ggcagccgat gatttgggcc gcgtcctgcg attcagggtt gaagtaagca acttcgaggc
     1741 tgcgtgccgc atgatatcgg cgcatgtggg aatcggcgtc atccccgagt ccgctgctca
     1801 gcgctacgcc agaaatcttc cgcttaaatt gattgcgcta aacgatgaat gggcattgcg
     1861 caagctccat atttgcgtca ggcgtctgga cagtttgccg gtgtttgccc aggagtttgt
     1921 ctctttatta gtgaaagacg ctgcgcaaac cgacgcgtgc gtcggcgcac gcgtgaatca
     1981 attgatgccc ggcaaatgaa ttcatcgaca cgttctacgc ctggccgcag aattgttctt
     2041 gcttcgaaca atgccggcaa gttgaaagag ttctcggcga tactgcaatc cgccggcgtt
     2101 gtcatggtgg ctcagggcga tttgggcgtt ccggaagcgg ctgagccttt cggcacgttt
     2161 gtggaaaatg cgctggccaa ggcgcgccat gcaagcgtta tcaccggctt gccggcactg
     2221 gctgacgact cgggattatg cgtgaatagt ttgaatggtg cgcctggcgt attttccgcg
     2281 cgcttttccg cgcttcacgg cggcgaaaaa tcagatgaag ccaataacca atgtctgctt
     2341 cgcctgctgc gtacgcaaaa caatcgatcc gcaagctata ttgccgtgct ggtgtatgtg
     2401 cgttcggccg cagacccctg tcccatcatt gccgaaggcg cctgggcggg cgaaataatt
     2461 gacgaacccc gcggattgca tggatttggt tatgatcctt acttttactt gcccaagctg
     2521 ggcaaaacgg cggcggagct aactccccga cttaaaaaca ggctcagtca ccgtgccagg
     2581 gctttaaaaa agctattgca agccttatca acccataacg agatctaagt ggctcctact
     2641 gtcgtgccgc gagttccaga taagccggct gcccgtccaa ccaccttggc cggcctcccg
     2701 cctttgtcct tgtatgttca tgtgccctgg tgcgtgcaca agtgtcctta ttgcgatttc
     2761 aattcccatg aacttaaagg cgagttgccc gaacgggact atctggccgc tttgcgtgcc
     2821 gaccttgagc aggcgcttcc gcttgtatgg ggccgccggg tggtgtcgat tttcatcggc
     2881 ggcggcacgc ccagcctgct ctcggcatcg gccatcgatc agatgatgac gctgtttcgc
     2941 tcgtgcctga atgtgctgcc cgatgcggaa gtcacgctcg aagccaaccc cggggtggcc
     3001 gaggcggcgc ggtttatgga gtatgccaac agcggtgtga atcgaatttc cctgggcgtt
     3061 caaagtttta acgaccagtc gctaagcgcg ctgggccgca ttcacgattc gaatcaggcc
     3121 cgcaaggcaa tcgggttcgc gcaaagagcc gtcgatcgcg tcaatctgga catcatgtat
     3181 gcgctgccgc gccaggcgct ggaagggtgc gccgacgatc tgcgcaccgc catgtcgttt
     3241 cggaccgaac acttatccct gtaccacctg acgttcgaac ccaacacggt ttttgccaaa
     3301 tatccgccga ctgtggccga tgacgacaca agcgcggcca tgcaggagtt gattgccgaa
     3361 cagaccagcc tgaacgggtg ggagcgctac gaggtgtccg cgtatgcgca gccgggcgcg
     3421 cgttgccgcc ataatctgaa ctattgggag ttcggcgact atctgggcat cggaccgggc
     3481 gcgcacggca agctatcctt ccatgaccgc atcattcgcc agtccaaatt aaaaaatccg
     3541 gcgtcatgga tggataaggc gcagcgaatg gataacagcc atatcgccga agaacgcgtg
     3601 gtgggcgccg acgaattggg ctttgaattc atgttgaacg cgctgcgttt aaagtcgggg
     3661 gtgtccgccg ccagttttac gcaacgcact ggtctggcgc tgcactcgat tcgcgcaccg
     3721 cttgacaggg cgattgcccg cggcctgctg gaatccgacc tcgctcgctt gcgggccacg
     3781 ccgctgggct ggaggtttct aaacgatttg caaatggaat tcttagaatg aatgcacgat
     3841 ttcatggatt gcactacaat ttgcaccaaa ttggtgcgtt acgccctttt ttcgtcaagg
     3901 cacattgcgc gtgcgcctgg gttgagtgtg atccaatttt agtaaaactg gcatagtttt
     3961 tgctttatag ctttttattc atcaacttga ttgtttaact tatcactgct caggagttca
     4021 tatgtccagt ccggaagacg ttgttaaatt aattgcagaa cgcgaagtgg cttttgtgga
     4081 ttttcgcttc accgacaccc tgggacgtga gcatcatatg acggttccgg ggcatacggt
     4141 tgacgaagaa aagcttgaat ccggacaggc gttcgacggt tcttccttgc ccggatggaa
     4201 aggaatcgag gcatcggaca tggttttggt tcccgatccc aacaccgcgc atatggatcc
     4261 gtttcgcgag gaaaccacac tgatcctgtc gtgcgatgtt gttgaaccgt ccgatttgaa
     4321 aggttatgac cgcgaccccc gctcgctggc gaaacgcgct gaagcgtatt tgaaatcaag
     4381 cggtttgggc gacacggcct attttggacc cgaacccgag tttttcatat tcgacggcgt
     4441 ggcatggaac gacgacatgt caggctgttt cgtgaaggtg aagtccgatg aggcgccctg
     4501 gtcgcgctca atcgatccgg acaatggcaa tttgggccat cgcccaaacg caaagggagg
     4561 gtatgtgccc gtgccgccgg ttgactcctt tcaggatatt cgctctgaaa tatgcctgct
     4621 gctggaacag cagggcgttc cagtcgaggt gcatcaccac gaagtggccg cccccggtca
     4681 actggaaatt ggcacgaaat tcagcacgct ggtgcagcgc gccgactgga accagatact
     4741 gaaatacacc atccacaacg ttgcctatgt ttacggtaaa accgccacct ttatgcccaa
     4801 acccattgtg ggcgataatg gttcgggcat gcatgtgcac cagtcaatct ggaaagacgg
     4861 tcaaaatttg tttgccggca acggctatgc aggcttgtct gaatttgcct tgtattacat
     4921 tggcgggatt atcaagcacg ctcgcgccct caatgcgatc accaacccgg gcacgaactc
     4981 gtacaaacgg ctggtcccgc attttgaagc gccggtcaaa ctggcttatt ccgcacgcaa
     5041 tcgctctgcg tcgattcgca taccctacgt caatagcccc aaggcgcgcc gggtggaaac
     5101 acgttttcct gatccgctgg ccaatcctta tctggctttt tcagccctga tgatggcggg
     5161 tctcgatggt gtgcaaaaca agatccatcc gggggacccc gccgataaaa atctttatga
     5221 tctgccgccc gaagaagacg caaaaattcc gacggtatgc gcgtcactcg acgaggcaat
     5281 tgccgctttg gatagcgatc gtgaattttt gactcgcggc ggtgtattca gcaacgatat
     5341 gctggacgct tatatcgaat tgaaaatggc cgagatcacg cgtttgcgca tgaccactca
     5401 tcccgtggaa ttcgatatgt attacagcct ttaatttcgc gccgcgatgg acctcgccgg
     5461 ctacgatatg ttggcaagca cgattttgct tctaaataat caaggcgagg tcgagtacgc
     5521 caatgctgcg gctcaagagt tgctgggttt gccgcgcaag cagttgacgg gtctgttcgg
     5581 gtattcatta ttcaaggccg acacggcgtt ggaatcacga tttctggacg ccataaacgg
     5641 gaaattcggg atattgcgtc aagacctgtt tgttgatcgg cttgggtcaa ccttgccggt
     5701 cagcctgacg attgtgccgt tggccgaccg cccttgggcg gcgttgcttg aagtgcgcgc
     5761 tgtggagcag catgtt
//
LOCUS       DATCXU010000467         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1062830,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000467 DATCXU010000000
VERSION     DATCXU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062830"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VIR04_10435"
     CDS             <1..1099
                     /locus_tag="VIR04_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY9461208.1"
                     /translation="GNRNFEGRIHPLAKASYLCSPPLVVAYALAGTMQVDLVREPLGT
                     GLDGKPVYLADIWPDPAELAGLVGSALNAKRFTDRYRGVFEGEKRWRALSVNNAQVFE
                     WQPDSSYMKQPPFFDSISLSMPPMPDIHSARVLVMLGDSITTDHISPVGSITADSAAG
                     QYLHSVGIKKAAFNSFAARRVNHDVMLRGVFANVRIRNEMMGGREGGWTRHIPSGEVL
                     PVHEAAKRYAEAGVPLVVIAGKDYGTGSSRDWAAKGTYLLGVRAVIAEGFERIHRSNL
                     IGMGVLPLQFPDGVSRITLGLDGTETVDIIGLDDMSPGCMLSCVMTRANGSQVKISLL
                     CRLDILLEVAYYRNGGILHYVARQMLADAPA"
     gene            1134..2108
                     /locus_tag="VIR04_10440"
     CDS             1134..2108
                     /locus_tag="VIR04_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY9461209.1"
                     /translation="MNTKKILRLLIAGAAVGLLPLGAHAEDWPSRPITFVVPFSPGGA
                     IDLISRVFAKQMGTDLKQSIIVENRPGAGGVVGSAHVARSKADGYTFLVAGNGIVTNS
                     QIRSDQPYKDDQLTPIAMLGVAPSIIITSPSNPAHSLKDFVENAKKNHVNRITFSRAG
                     VGSTPDFVGAMVKLETGLPVEPISYKSGSEDVTAVMSGQVDMTSEASIVTLPLIKAGK
                     VKALATTWGGVLASAPNIPTTAQEGFPNLRIGHWEGLFAPAGTPNAILDAMNAAVLRA
                     AKMPDVLNVLEKSSVEPGSYTRPEFVQFTEGERTRLGKVVRQAHMRAE"
     gene            2171..>2596
                     /locus_tag="VIR04_10445"
     CDS             2171..>2596
                     /locus_tag="VIR04_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY9461210.1"
                     /translation="MNNAAPRNASFNSVRGRPHIVILGGGFAGLWAARALNGAPVRVT
                     LIDRVNHHVFQPLLYQVAGAGLSGTDIAAPLRHILRHQRNITTWMEEVTDIDLAARRV
                     HAGGRDQDYDSLIVATGSTHAYFGHDAWAVHAPGLKTMND"
BASE COUNT          567 a          726 c          769 g          534 t
ORIGIN      
        1 gggaaatcgc aatttcgagg ggcgtattca ccccttggcc aaagcaagct acctttgttc
       61 cccgccactc gttgtagcct acgcgctagc cggaaccatg caggtggacc ttgtgcggga
      121 gccactcgga accgggcttg atggcaagcc ggtgtatctg gccgacattt ggccagaccc
      181 cgcggagctg gccggtttgg tgggatcggc gctgaatgcc aaacgtttta ctgacaggta
      241 tcgcggcgtg ttcgaaggag agaagcgctg gcgcgcactt tcagtaaaca acgcccaggt
      301 atttgaatgg cagccggata gcagctacat gaagcaacct ccgtttttcg acagtatttc
      361 cttgagcatg cctcccatgc cggacattca ctcggcacgt gtacttgtga tgctgggcga
      421 ttccattacg acggatcata tatctccggt aggcagcatt accgccgact cggctgctgg
      481 ccaatatctt cacagcgtgg ggataaaaaa agcagccttc aattcattcg cggcacgacg
      541 ggtgaaccac gatgtgatgc tgcgcggagt cttcgcgaat gtccggatcc gcaatgaaat
      601 gatgggcggc cgggaagggg gttggacacg ccatatccca agtggagaag tgttgccggt
      661 acacgaggcg gctaaacgat acgctgaagc cggcgtgcct ttggtggtga ttgcgggaaa
      721 agattacggc acaggctcct ctcgggactg ggctgcgaaa ggaacttatt tgctgggcgt
      781 gcgcgcggtt atcgccgaag gcttcgagcg tattcatcgc tcaaacctga taggaatggg
      841 cgtgctgcct ttacagttcc cggacggagt atcgcgcatt acacttggcc tggatggtac
      901 cgaaacagtt gacatcattg gcctggacga catgtcgcct ggttgcatgc tttcgtgcgt
      961 gatgacccgt gccaacggta gccaggtaaa aattagtttg ctttgccggc tcgatatctt
     1021 gcttgaagtt gcctactacc gtaacggcgg aatattgcac tacgtcgcaa ggcagatgct
     1081 ggcggacgcg ccggcttaac actatccaac ccgccacaac tcaggagaac ggaatgaaca
     1141 caaaaaagat attgagattg ctgattgccg gcgccgctgt cggactactc ccgctcggcg
     1201 cgcacgcgga agactggcca agcaggccaa tcacattcgt cgtgccgttt tcacccggcg
     1261 gtgcgattga tttgatttca cgcgttttcg ccaaacagat gggcacggac ctcaagcaga
     1321 gcattattgt agagaaccgt ccgggtgcgg gaggcgtggt cggctcggca catgtggcac
     1381 gcagcaaagc ggacggctat accttcctgg tagccggcaa cggaatcgtc accaatagcc
     1441 aaatacgctc cgaccagcct tacaaagatg atcagttgac gcccattgcc atgttgggcg
     1501 tggcgccctc catcattatc accagcccgt caaatcccgc ccacagcctt aaggacttcg
     1561 tcgaaaacgc caagaaaaat cacgtcaacc gcatcacctt ctcacgtgcg ggcgtgggca
     1621 gcacgccgga tttcgtcggc gccatggtca agcttgagac agggttgccc gttgagccga
     1681 tctcctacaa gagtggttcc gaggacgtca ctgccgtcat gagcggccaa gtcgacatga
     1741 cttcagaagc aagcatcgtc acgttgccgc tgatcaaggc gggcaaggtc aaggcacttg
     1801 ccaccacctg gggaggcgtt ctggcaagcg ccccaaacat cccaactact gcccaggaag
     1861 gtttccccaa tctgcgtata ggccactggg aggggttgtt cgcgccggcg ggcactccaa
     1921 acgcgattct cgatgcaatg aacgcggcgg tgctgcgggc agccaagatg cccgatgtct
     1981 tgaatgtact ggagaaaagc agcgtagaac ccggctccta tacacggccg gagttcgtcc
     2041 agttcaccga aggcgagcgg acccggctcg ggaaagtggt aaggcaggca cacatgcgcg
     2101 cggaatgagg ggtttgccgg aattccaatt acatgacggc atgaatttct caatttagag
     2161 agcaacacag atgaacaacg ccgcccctcg caatgcctca ttcaactccg tgcggggacg
     2221 gccgcatatt gttattcttg gtggcggctt cgcaggcctg tgggccgccc gggcgctgaa
     2281 tggcgcgccg gtgcgcgtga ccctgattga tcgcgtgaac catcatgtgt ttcagccttt
     2341 gctgtatcag gtggccggag caggtctctc gggcaccgat attgcagcgc cattgcggca
     2401 tattttgcgc caccagcgca acatcacgac gtggatggag gaagttacgg acattgatct
     2461 cgccgcgcgc cgcgttcacg cgggtggccg cgatcaggac tatgacagcc ttatcgtcgc
     2521 gactggttcg acccacgctt actttgggca cgacgcgtgg gcagtgcatg cgcccggcct
     2581 gaagacaatg aacgat
//
LOCUS       DATCXU010000468         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_837611,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000468 DATCXU010000000
VERSION     DATCXU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837611"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VIR04_10450"
     CDS             complement(<1..279)
                     /locus_tag="VIR04_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY9461211.1"
                     /translation="MSNSVFAERIDALRAVMATRKVDAYIVVSSDPHLSEYLPEHWQA
                     RQWLSGFDGSAGTLVVTARFAGLWTDSRYWVQAEHDLNGTGIQTIRAGD"
     gene            complement(514..1167)
                     /locus_tag="VIR04_10455"
     CDS             complement(514..1167)
                     /locus_tag="VIR04_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY9461212.1"
                     /translation="MAWNRDEMAARAARELQDGFYVNLGIGIPTMVANHVPAGMEVWL
                     QSENGLLGIGPFPTEDEIDADLINAGKQTVTTLPGSSIFASADSFGMIRGGKINLAIL
                     GAMQVSETGDLANWMIPGKMIKGMGGAMDLVAGVGKVVILMDHVARKKDGTEDIKLLP
                     KCTLPLTGVGVVNLIITDLGVMEVTDKGLKLTELAPGVTVAEVQAKTRAKLDVSAVA"
     gene            complement(1167..1877)
                     /locus_tag="VIR04_10460"
     CDS             complement(1167..1877)
                     /locus_tag="VIR04_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY9461213.1"
                     /translation="MNKVYASAQEALKGVVANGQMIAVGGFGICGIPEALIAALRDSG
                     VQNLTCVSNNAGIDDFGLGILLRTRQIKKMIASYVGENKEFERQFLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFYTRTGVGTVVAEGKEVHEFNGEPYIMERSLVPDVSLVKAYMA
                     DRSGNLVFRKTARNFNPNVAMAGKITIAEVEKLVDTGTFDPDAVHLPGIYVHRIVVNA
                     HPEKRIEKRTVREASGDK"
     gene            complement(1942..2382)
                     /locus_tag="VIR04_10465"
     CDS             complement(1942..2382)
                     /locus_tag="VIR04_10465"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY9461214.1"
                     /translation="MTLESQSSMLPIGGIFRCVLPVRWGDMDALNHVNNTLYFRYIEE
                     ARVRLLAQAGIVLPSNRVGVLAHASCDFLKPLHYPANVVVSQILTQIGRSSMDVDVVI
                     ECQDEPGVVYAKGRAVMVGAMAATGKSSPWTADELARFAKCFKE"
BASE COUNT          595 a          795 c          624 g          573 t
ORIGIN      
        1 gtcgccggcg cgtatggttt gaatgccggt tccgttcaag tcatgctccg cttgcaccca
       61 atagcgactg tcggtccaca atccggcgaa cctggcagtg acgaccaggg tgccagcgga
      121 gccatcgaag ccgcttagcc actggcgagc ctgccaatgc tcgggcaggt actcggaaag
      181 gtgcgggtcg gaggacacaa cgatgtacgc atccactttc cgggtcgcca tgacggcgcg
      241 cagggcatcg attcgttcag caaaaactga attgctcatt taattccttt tgtatatttc
      301 tggctggtgc gtattgggtc aatttagtga cgatcggtga tggcggggtt ctttgagtca
      361 acttagctgt ggcggggtag cggtcattac gtacgaaaag atcgacgggc cacccgtcga
      421 tcttttcgta cgcaatacta actgagccgg cgcagcgcaa tactttcacc gcggcgcaac
      481 gaaatacatt cactgcggcg cacgcacccc tagttaagca accgccgaaa cgtccagctt
      541 tgcccgagtc ttcgcctgca cttccgccac tgtcactccc ggcgcaagtt cggtaagctt
      601 caatcccttg tccgtgactt ccatcacccc aagatcagta atgatcaagt tgaccacccc
      661 cactccggtc aagggcaacg tgcacttggg cagcaacttt atatcttcgg tgccatcctt
      721 cttgcgtgca acgtgatcca taagaatcac caccttgccc accccggcca ccagatccat
      781 cgcgccaccc atgcccttga tcatttttcc cggaatcatc caattggcca ggtcgcccgt
      841 ttcggatacc tgcatggcgc ccagaatagc caggttgatt tttccgccgc gtatcatgcc
      901 gaacgaatcg gccgaggcga agatcgatga ccctggcaag gtcgtaaccg tttgcttgcc
      961 ggcgttgatc aggtcggcgt caatttcatc ttcggtgggg aacgggccaa tgcccagcag
     1021 cccgttttcc gactggagcc acacttccat cccagcgggc acatggttgg caaccatggt
     1081 cggtatgcca atgcccaggt tcacataaaa accatcttgc aactcacgag cggcgcgcgc
     1141 cgccatttca tcacgattcc aagccattac ttatctcctg aagcttcacg cacagtacgt
     1201 ttttcaatcc gtttctcggg atgggcgtta accacgatcc gatgaacata aatacccggc
     1261 aaatgcacgg catcgggatc aaaagtaccc gtatcaacca atttttcgac ctcggcgatg
     1321 gtaattttgc ctgccatcgc tacgttggga ttaaagttgc gcgccgtctt gcgaaaaacc
     1381 agattgccgc tgcgatcggc catataggct ttaaccagcg atacgtccgg aaccagcgag
     1441 cgctccataa tgtacggctc gccattgaac tcgtgcactt ccttgccctc ggccaccacc
     1501 gtgcccacgc cggtacgcgt ataaaacgca ggaataccgg cgccgccagc gcgcaatttc
     1561 tccgccagtg ttccttgcgg cgtaaattca agttccagtt cgccggccag gaactggcgc
     1621 tcgaactctt tattctcgcc cacatacgag gcgatcattt tcttgatctg acgggttcgc
     1681 aagagaatac caaggccgaa atcatcaatt cccgcgttat tgctgacgca agtcagattt
     1741 tgcacgcccg aatcccgcaa cgcggcgatc aaggcctcgg ggatgccgca aattccgaac
     1801 cctccgaccg cgatcatctg accgttggca acgacgcctt tgagcgcttc ttgcgcactc
     1861 gcatagactt tattcattac tccaatcctt taaaattacg cagttcaaac cgacgacagc
     1921 cactgaagca cttctattgg ctcattcttt aaaacatttc gcaaatcgcg ccagctcgtc
     1981 tgccgtccag ggcgaagact tgccggtagc cgccatggcg cccaccatta cagctctgcc
     2041 ttttgcataa actacgcccg gctcgtcctg acattcaatc acaacgtcga catccatgct
     2101 ggaccgccct atttgcgtca gaatctggct gaccaccacg ttcgcaggat aatgcaacgg
     2161 ctttagaaaa tcacacgagg catgggccag cacgccgacc cgatttgagg gcagcacaat
     2221 gccggcctgc gcaagcaacc tgacacgcgc ttcttcaatg taacgaaagt aaagcgtgtt
     2281 gttgacatga ttcagcgcgt ccatatcgcc ccatcgcacc ggcaggacac agcgaaaaat
     2341 accgccaatg ggcagcatgg atgattgact ttcaagcgtc atggcgcaca cgccctctca
     2401 atggaatctt tcaagtataa cggcaaggaa aaggccgccg gcccaaccgg cccaatctct
     2461 gtacacaact tcaataatgc cctttacaat tacgttcgaa gcacacgaca tcaacaggag
     2521 attctatcca tggctgatcg cgaatcaatg gactacgacg ttctcatcgt cggcggcggg
     2581 ccggcgg
//
LOCUS       DATCXU010000469         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1069364,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000469 DATCXU010000000
VERSION     DATCXU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069364"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VIR04_10470"
     CDS             complement(<1..685)
                     /locus_tag="VIR04_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY9461215.1"
                     /translation="MPLRDPSFGDVGQIYDPWVWLGYITAATSRIALATGSIVLALCH
                     PLDVAKAAASIDNLSDGRFVLGVATGDRPVEFPAYGRQIEQRGALFVESLAYIRRALQ
                     VSFPAINSPFGTLSGADLLPKPSRGRIPIAVTGHIQQSIDWIAANSDAWIMYPRTPVV
                     QAKVVQSWNEAVERNGQRPTKPFAQSLYIDLTEDANAPPQPIHLGFRLGRTALIDLLH
                     TLEEIGVNHV"
     gene            complement(713..1657)
                     /locus_tag="VIR04_10475"
     CDS             complement(713..1657)
                     /locus_tag="VIR04_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY9461216.1"
                     /translation="MPATSSKRLYIQEVAPRDGFQNEHTFIPTEQKIALINALGRCGL
                     AKIEASSFTSPRAIPALADAEAVMQQIERIPGVIYTALIPNVRGAQRALECAVDEMNL
                     VMSVSETHNRVNLRMPREKSFAQLSEVIAMAAAHNVPVNVSLSTAFGCPMEGAVAEAE
                     VYAWIERFAALSIRGVTLCDTTGMANPSQVGCISRQVRERFPKLEITLHFHNTRGMAL
                     ANAVAAVDAGIDRFDASLGGLGGCPYAPGATGNVCTEDLVHMFSEMGCDTGVDLDSLL
                     AAARQLPALIGHDVPGQVLKAGPSSRRYPAPASVTQNS"
     gene            complement(1673..2866)
                     /locus_tag="VIR04_10480"
     CDS             complement(1673..2866)
                     /locus_tag="VIR04_10480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9461217.1"
                     /translation="MSTLPLDGIRVIELGQLIAGPFAAKMLGEFGAEIIKIEPPGSGD
                     PLRKWRLLHNGTSVWWQVQSRNKQSVTLDLRQPEGREVVKKLVAEADVLIENFRPGTL
                     EGWGLGWDTLQAINPGLIMLRVSGYGQTGPYRDRPGFGVVGEAMGGLRYLSGEPGRTP
                     VRAGVSIGDSLSALHGVIGVLLALLHRVKSGGAGQVIDVALYESVFNIMESLLPEYSA
                     FGVVREPAGSSLPGIAPSSAYPCKDGRYALVAGNGDSIYKRLMHVIGRDDLGEDPALA
                     TNDGRVARVAEIDGAIGRWTLQHNLEDVLTILNAARVPVGRIYSAADIAADPHYRARD
                     MILQSTTPDGKTIDVPGIVPKLSVTPGGVRSEAPGLGQDTDRVLESLGIDAATRAEWR
                     KRQII"
     gene            3144..4040
                     /locus_tag="VIR04_10485"
     CDS             3144..4040
                     /locus_tag="VIR04_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY9461218.1"
                     /translation="MDDWIELLAAEGISFDPVRWAPDNPPSGAEVVITRFPEDEMFRS
                     ETNLKIVFNMTAGVDGVLRMKSLPIDVPIIRLEDSGMAKKMAEYAIYAIAEISRDMDK
                     YRQAKKDKKWLPEPRSDVEDWPVGVMGLGIIGREVATQVARIGYPVSGWARSRHALEA
                     VSCYAGEAGFNDFLSHTRILINVLPVTPQTENIMNKQTFDRLLPDGYIINMGRGQHLD
                     ENALTESIANGVLGGAVLDVFRTEPLPASHPFWTNPAISITPHVSGKTDMKAAIKQVA
                     RKFRAWQSGEKLSGMVDRSVGY"
BASE COUNT          842 a         1189 c         1335 g          881 t
ORIGIN      
        1 ctacatggtt cacgccgatc tcttcaagcg tgtgcaaaag atcgatcagc gccgttcgtc
       61 ctagcctgaa gccaagatgg atcggttgcg gcggtgcatt ggcgtcttcc gtcaggtcaa
      121 tatataggga ttgcgcaaac ggtttggtgg ggcgttgtcc gttgcgttcg acggcttcat
      181 tccaggactg cacaacttta gcttgtacga caggggtgcg cggatacatg atccaggcgt
      241 ccgagttggc ggcgatccaa tcgatgcttt gctgtatgtg tccggttacg gcgatgggga
      301 ttcggcctcg tgatggcttg ggtaacaagt cagcgccgga cagtgtgccg aacggcgaat
      361 tgatagcggg aaagcttact tgcaacgcgc gccggatgta tgcaagcgat tcgacgaaca
      421 gtgcgccgcg ttgttcgatc tgacgcccat aggcggggaa ttcgaccggc cggtcgccgg
      481 tggcgacgcc cagcacgaaa cgcccatcgg aaaggttgtc gatggacgcc gccgccttcg
      541 cgacatccag cggatggcat agcgctaata cgattgaacc ggttgccagc gcaatccggc
      601 ttgtggcggc cgtgatatag cctaaccaca cccacgggtc gtagatttgg ccgacatcgc
      661 cgaaactcgg atcgcgtagc ggcacgtccc gagttctgtt tcctttcggt attcaactgt
      721 tttgggtcac cgacgcaggc gcggggtagc gcctgctgct gggcccggct ttaagcacct
      781 gcccaggtac atcgtgacca atcaacgccg gcaattggcg cgcggccgcc agcagactgt
      841 ccagatcgac accggtgtca cagcccattt cgctaaacat atgcaccagg tcttcggtgc
      901 agacgttgcc cgtggcgccg ggcgcatacg gacagccgcc caaaccgccc agcgaggcgt
      961 cgaatcggtc aatgcctgcg tcgaccgccg cgacggcgtt ggccagcgcc atgccgcgcg
     1021 tgttatgaaa atgcagagtg atttccagct tggggaaacg ttcgcgaacc tgccggctta
     1081 tgcacccgac ttgcgacggg ttggccatgc ctgtggtgtc gcacagtgtg acgccgcgta
     1141 ttgacagcgc ggcaaagcgc tcgatccagg cgtagacctc ggcttcggcg acggcgcctt
     1201 ccattgggca gccgaatgcg gtggaaaggg aaacgtttac gggcacgtta tgggcggcgg
     1261 ccatggcgat gacttcggaa agctgggcga atgatttttc gcggggcatg cgaaggttga
     1321 cacgattgtg cgtttcgctg acggacatga ccaggttcat ttcatcgacg gcgcattcca
     1381 acgcgcgttg ggcgccgcgc acattcggaa tcaacgcggt gtagatgacg ccgggtatgc
     1441 gctcgatctg ctgcatgacg gcttcggcat cggccagggc cggaatggcg cgcggcgatg
     1501 tgaaagagct tgcctcgatt ttggccaaac cgcaacggcc cagcgcgttg ataagcgcga
     1561 ttttttgttc ggtcggaata aaggtgtgct cgttttgaaa accatcgcgc ggcgcgacct
     1621 cctggatgta caaacgcttg gacgaagtgg caggcatggc gggctctcga agtcagataa
     1681 tttgacgttt gcgccattcg gcgcgggtgg cggcgtctat tcccagcgac tcaagcacac
     1741 ggtccgtgtc ctggcccagg ccgggtgctt cgctgcgcac gccgcctggc gtaacgctaa
     1801 gcttgggcac gatgccgggc acgtctatgg ttttgccatc gggcgtggtg ctttgcagaa
     1861 tcatgtcgcg ggcgcggtag tgcgggtcgg cggcaatgtc ggcggcactg tagatgcgtc
     1921 ccactggcac gcgcgcggcg ttcaaaatag tgagcacgtc ttccagattg tgttgcaggg
     1981 tccagcgccc gatggcgccg tcgatttcag cgacgcgcgc gacgcgtcca tcgttggtgg
     2041 ccagcgcggg gtcttcaccc aggtcgtcgc gcccgatgac gtgcattaac cgtttgtaaa
     2101 tgctgtcgcc attgcccgcg accagcgcgt agcggccatc tttgcagggg tacgcactgc
     2161 tgggggcgat gcccggcaag ctgctgcccg ccggttcgcg caccacgccg aaggcgctgt
     2221 attcgggcag caggctttcc attatgttga ataccgactc atatagcgcg acgtcgataa
     2281 cttgtccggc gccgccggat ttgactcggt gcagcagcgc aagcaggacg ccgatcacgc
     2341 cgtgcagcgc cgacagtgaa tcgccgatcg acaccccggc gcgcacgggg gtgcggcccg
     2401 gttcgcccga caggtagcgc aggccgccca tggcttctcc gaccacgccg aagccgggcc
     2461 ggtcgcgata agggcctgtc tgaccatagc ccgagacgcg cagcatgatc agccccgggt
     2521 tgatcgcttg caacgtgtcc cagcccaggc cccagccctc gagcgtgccg gggcggaaat
     2581 tttcgatcag cacgtcggct tcggccacca gctttttaac gacttctcgt ccctcgggct
     2641 gacgcagatc aagcgtgacc gattgcttgt tgcgcgattg cacttgccac cagactgacg
     2701 tgccgttatg cagcagacgc catttgcgta aagggtcgcc gctgccgggg ggttcgatct
     2761 tgatgatttc ggcgccgaat tcgcccagca ttttcgcggc aaacgggccg gcgattaatt
     2821 ggcccaactc aattacgcga attccatcca gtggaagcgt gctcatgttt attcgttcct
     2881 gttgctctgt caccaacgca atcatggcgg aaaattgccg ggtaaagcgg cggaaggaag
     2941 ggattgcgtg atagagacga tgttaacatt cccgatgctg tgggtcttgc atggcgcgtt
     3001 tcttcaatga atcggcttaa tcgggcgggg ccagcgtaac actatgtttg agtccagacg
     3061 cgatgtcggc gagtggaaac ttttttagca caaacaggaa gtttaaattg cagatacttt
     3121 tttattcgcc ggactcggaa agtgtggatg actggattga attactggcc gcggagggaa
     3181 tcagcttcga tcctgtaagg tgggctccgg acaacccccc aagcggcgcc gaagtcgtga
     3241 ttacccgctt ccccgaagac gagatgtttc gcagcgagac caatcttaaa attgtgttca
     3301 acatgacggc gggcgtggac ggcgtgctaa gaatgaaatc gttgccgatc gacgtgccga
     3361 tcatacgcct ggaagattcg ggcatggcga agaagatggc ggaatacgcc atttacgcta
     3421 ttgcggaaat cagtcgcgat atggacaagt accggcaggc gaagaaagac aaaaaatggc
     3481 ttcccgaacc gcgcagcgat gtggaagatt ggcccgttgg cgtaatgggc ttgggcataa
     3541 tcgggcgcga agtggccacc caggtggcgc gaatcggtta tccggtgagc gggtgggcgc
     3601 gctcgcgcca tgccctggaa gcagtctcgt gctatgccgg tgaagccggt tttaacgatt
     3661 tcctgtccca cacgcggata ttgatcaacg tgttgccggt aaccccacag accgaaaata
     3721 tcatgaataa gcagacgttc gatcgcctgc ttccagacgg ctacattatt aacatggggc
     3781 gcggccagca tctggacgaa aatgcactga ccgagagcat cgccaacggc gtgctgggcg
     3841 gcgcggtgct ggatgttttc cgcacggaac ctttgcctgc aagccatccc ttctggacga
     3901 accccgccat cagcatcacc ccgcacgttt cgggcaaaac ggacatgaaa gcagcaataa
     3961 aacaggtcgc ccgaaagttc agggcctggc agtcggggga aaaactaagc ggtatggtgg
     4021 atcggtccgt ggggtattaa gcgacgacgg ggcgccgggc ccgttacgct ttaacgtccg
     4081 gcgggaataa atccgtcgcg gttcggcatt ttgacttttg ccagcgtgtc ctggtccatt
     4141 acggcgtcgg cgccaatgat gtttcctttg gacagaatgt acgccgacaa cgcgtaaacc
     4201 tggtcatcgg tcagctttcc gggcgtgtaa aaaggcatgg ctctttt
//
LOCUS       DATCXU010000470         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_606320,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000470 DATCXU010000000
VERSION     DATCXU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606320"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VIR04_10490"
     CDS             complement(<1..1838)
                     /locus_tag="VIR04_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY9461219.1"
                     /translation="MNTSLTTLPPSALSPYSPPPAPPENDSVDFQNLFRIISDARWLI
                     LTMAAGALLLGGAYALFSPPVYQADTLIQVEQAQNSTNDNVLNQLNTAFNVQSSASAE
                     MEILRSRMIIGQTVDKLGLAISATPNYLPIIGEWMARRATQLSKPGLFGYGGYVSGAE
                     SIKIGQLNVPPELLGKKLTLTATTRGYELRGPDNQKLANGTVGTPAAFRPGPGHILIS
                     DLHAEPGARFTVMRQARLRAIDSLQGRLSITEKNKPSGVLSVSLQGADPAQVALILNS
                     IGSAYAQQNTERKAAEAEKSLAFLDNSLPQLKRQMDASENEYTRFRDQHGTFNLTAEG
                     TLSLNTSAALQAQLFDLQQKRRALTAQFSDGYPGVRAIDQQIAAAKEQIDKLAARIKS
                     LPDLERRLVNLTRDVKVNSDLYANLLNSAQQMRLVKEGKVGNVRVIDAAVVPMSPVKP
                     DPLLVLAISGTAGLTLGIILALLRNWKHPSIKTVDDIEINLGLNVFATIPHSLPQAKM
                     HQQLGNEASGNQVLALIAPDDPAIESLRSLRTTLQFLLRGATNNIVVLTGPTPNIGKT
                     FVGVNFAALLGAAGKRVLLIDADFRRGYLHQYFGLGRRKGFSELIGG"
     gene            complement(1847..3007)
                     /locus_tag="VIR04_10495"
     CDS             complement(1847..3007)
                     /locus_tag="VIR04_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY9461220.1"
                     /translation="MITSPSFSAFQKLRRGVYICAAIGAAFVLSACSVAPGMYMGTPK
                     DVSVTLKDGTAPPGALTPITADLIKQQQSSQAEPIPQQVKDLFGTGEPYQIGPGDILN
                     IVIWEHPELAMTSASSNLSTGSPNQADVGNGYNVSADGRIQFPFLGPIKVAGLTEYQV
                     RNLITRRIGAFVNDPQVTVRVQSYRHGRVYMDGEVRTPGLQTMNDIPLTLPEALNRAG
                     GFTIDADRSSIMLTRKNVTTRINLPQLTRDGINPQSILLENGDLIRVANRNDSKVYLL
                     GEVLRTNSQVMRDGELTLGDALGEAGGVTPESSNPRQIYVIRKGSQGHAEIFHLNAAS
                     PVSYVLAAGFELKSHDIVFVDPAPIVRWNRVISMLLPSYGAVFTGAAVTNRR"
BASE COUNT          631 a          821 c          911 g          710 t
ORIGIN      
        1 ccgccgatga gttcactgaa tcctttgcgc cgccccagcc cgaagtactg atgaagatag
       61 ccccgccgga aatcggcatc gatgagcagt acccgtttgc cggccgctcc cagcagcgcc
      121 gcaaaattga caccgacgaa cgtcttgccg atattgggtg tcgggccggt taacacaacg
      181 atgttgttgg tggcgccgcg caagaggaat tgcagggtcg ttctaaggct gcgcaaactc
      241 tctatggccg ggtcatcggg cgctataagg gccagcacct ggtttccact ggcttcattg
      301 ccaagctgtt ggtgcatctt ggcttgcgga agcgaatgcg gaattgttgc gaacacattc
      361 aggcccaaat tgatttcgat atcgtcaacg gttttgatgc tggggtgttt ccagtttcgc
      421 aacagagcca ggataatgcc tagcgtcagc ccagccgtcc cggaaatggc cagcaccaaa
      481 agcgggtcgg gcttgacggg gctcatggga accacagccg cgtctataac gcgcacattg
      541 ccgaccttgc cttctttgac tagacgcatc tgctgtgcgc tattaagcag gttggcgtat
      601 agatcactat ttacttttac atcgcgagtc agattaacca gtcttcgttc cagatccggc
      661 aggctcttga tgcgggcagc cagtttgtca atctgctctt tggcggccgc gatttgttga
      721 tcgatggcgc gaacgccggg atagccatcc gaaaattgcg ccgtcagtgc gcgccgcttt
      781 tgctgaagat cgaacaactg agcttgcagc gcggcggaag tgttcagcga cagggtccct
      841 tcagccgtta gattgaacgt tccgtgctga tcgcggaatc gcgtgtattc attttcagac
      901 gcatccattt gtcgtttcaa ctgcggcaaa gaattgtcca gaaaggcaag cgatttttcc
      961 gcttccgccg ctttacgctc cgtattttgt tgtgcataag ccgatccgat tgaattgagt
     1021 atgagtgcca cttgggccgg gtcggctccc tggagcgaaa cggacagcac tcccgacggt
     1081 ttgttttttt cagtgatcga gagtcgtcct tgcaagctgt cgatggctct cagacgcgct
     1141 tgccgcatca ccgtgaagcg cgcaccgggc tcggcgtgaa ggtcgctgat taaaatatgg
     1201 ccggggccgg gcctgaaggc ggccggtgtg cccaccgtgc cattggccag cttttggtta
     1261 tcggggccgc gcagttcata acccctcgtg gtggcggtca gggtgagctt ttttcccagc
     1321 aattcgggcg gtacattgag ttgcccgatc tttatcgact cggcgccgga gacgtaacca
     1381 ccatagccaa agagcccggg tttcgacaat tgggtggcgc gtcgagccat ccactccccg
     1441 attatcggaa gataattggg ggtggcgcta atagccagcc ccagcttgtc aaccgtctgc
     1501 ccgatgatca tgcgtgaacg caagatctcc atttcggccg aagcggatga ctgcacatta
     1561 aaagcggtat tcaactgatt taacacgttg tcattggtgc tgttctgggc ctgctcgact
     1621 tgtatgagcg tgtcggcctg atacaccggt gggctgaaaa gagcataggc cccgcccagc
     1681 agcaaggcgc ctgcagccat cgtcagaatt agccagcgcg catcacttat tatccggaac
     1741 aggttctgga aatcgacact gtcgttttcc ggtggagcgg gcggaggcga atagggcgaa
     1801 agggccgaag ggggcaaggt agtgagagaa gtgttcattt tcgtggttac cttctgtttg
     1861 tcacggcggc gccggtgaac accgcgccat agctcggcaa cagcatgctg attacgcggt
     1921 tccaacggac aattggcgcc ggatcaacga agacgatgtc gtgagatttc agctcgaatc
     1981 cggccgccag cacataggag accggcgagg cggcattcag atggaaaatc tcggcatggc
     2041 cttgactacc tttgcgtatg acatagatct ggcgtgggtt ggaggactct ggtgttactc
     2101 cgcctgcctc gcccagtgcg tccccgagag tcagttcgcc atcgcgcatg acttgcgaat
     2161 ttgttctcag gacttcaccc agcaggtaca ctttggagtc gttgcgattg gcgacccgta
     2221 tcagatcgcc gttttccagg aggatcgatt gcggattgat accgtcgcgg gtcagttgcg
     2281 gcaggttgat tcgggtggtg acgtttttgc gggtaagcat gatggacgat ctatcggcat
     2341 cgatcgtgaa gccgcccgcc cgattcaacg cctctggcag ggtcagcgga atgtcgttca
     2401 tggtttgcaa gccgggcgtt cgcacttcgc catccatata cacacggccg tgacgatagg
     2461 attggacgcg tacggtcact tgagggtcgt tcacgaacgc tccaatgcgc cgggtgatca
     2521 agttgcgcac ctggtactcg gtcaggcccg cgactttgat gggccctaaa aagggaaact
     2581 gaatacggcc atcggcactg acgttatagc cgttacccac atccgcctga ttgggcgaac
     2641 cggtgcttag attggagctg gcggaagtca tggccagttc aggatgctcc catatcacga
     2701 tattcaggat gtctcccggc ccgatctggt agggctctcc cgttccgaag agatctttga
     2761 cctgttgcgg aattggctct gcctggcttg attgctgctg tttgatcaga tctgccgtga
     2821 ttggagtcaa cgctcccgga ggggccgtgc catcttttag cgtgaccgat acgtcttttg
     2881 gcgtgcccat gtacatgccc ggcgcaacac tgcacgcgga aagcacgaac gcggctccga
     2941 tggctgcgca aatgtagacg ccacgccgca gtttttgaaa agccgagaaa gaaggggaag
     3001 tgatcataat agggctcaag cgtagggact ggaaggaatt ttcagaacaa gcaacagtcg
     3061 cgggcaggct gcc
//
LOCUS       DATCXU010000471         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1040958,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000471 DATCXU010000000
VERSION     DATCXU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040958"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /gene="pcnB"
                     /locus_tag="VIR04_10500"
     CDS             complement(<1..1302)
                     /gene="pcnB"
                     /locus_tag="VIR04_10500"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY9461221.1"
                     /translation="MLTETIKSFASRLFSKPARGPQRYTRDKHGIDLRNVSRHAIKVC
                     EVLHEHGFDAYIVGGAVRDLIVGLEPKDFDVATNATPNQIRPLFRRARIIGRRFQLVH
                     VVFGQEIIETSTFRAPTTADQETDENGRILRDNLFGTHEQDAARRDFTLNALYYDPIK
                     EQVIDFHGGVGDLKKRVVRVIGDPNTRYREDPVRMLRALRFAAKLNATLDPSTREPIK
                     SLANLIEGVPASRLFDEMLKLLTCGHAMDCLQRLRAVGLHQGMLPLIDLIFEETGGQQ
                     FVELALERTDTRVRAGKPISPSFLFAALLWTLVNKRWLALMESGEPRIQALMQAADSV
                     VEEQTKKLAIQRRFQADMREIWFMQPRFERATAKTIWRMLEQPRFRAAVDFLQLRAAT
                     REVDSVLAQWWMDLANADDEKRAAMIEAYQRENKGSTGTRRR"
     gene            complement(1311..2012)
                     /locus_tag="VIR04_10505"
     CDS             complement(1311..2012)
                     /locus_tag="VIR04_10505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY9461222.1"
                     /translation="MTLSKRLALFDLDHTLLPLDSDYQWADFLARTGRAGDPIEAQRR
                     NNDLMERYNAGDLTAEQSAAFMLGLLAQGHAIELAEWHEHYMTEVIRPAILPVAQNLV
                     QSHLKQGDLCAIVTATNQFVTAPIARAFGVPHLIATQPEYIEGRYTGRISGTASFKEG
                     KVLRVNQWLASMGMALKDFERSYFYSDSNNDLSLLEAVTHPIATNPSPALRRIAENRG
                     WRILELFKDTLDAKS"
     gene            complement(2009..2716)
                     /gene="hda"
                     /locus_tag="VIR04_10510"
     CDS             complement(2009..2716)
                     /gene="hda"
                     /locus_tag="VIR04_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEY9461223.1"
                     /translation="MTQQLILDLLSTPTPTLENFVVGKNQAVLDALNSCEPGRALYLW
                     GAQGAGRTHLLRALGTMPRALYFGVADRADTLKDIATGDSVALDLIAIDDVGQLNEHA
                     QSAVFALYNRWREVSSTRRAFVLLLAGDQAPLAMPLREDLRTRLGWDLVFRLEPLSDG
                     DLAVAFETRANEKGLKLTPEITNWVLTHYARDMGRLGALLDALDRYSLEKHRAITLPL
                     LKDLLSMTPMPAAPLHS"
     gene            2880..>3016
                     /locus_tag="VIR04_10515"
     CDS             2880..>3016
                     /locus_tag="VIR04_10515"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905501.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY9461224.1"
                     /translation="MTKKHSAPLTYLDAGVDIDAGEALVDRIKPLAAKTMRAGVMAGI
                     GG"
BASE COUNT          619 a          828 c          905 g          664 t
ORIGIN      
        1 ccggcggcgc gtccccgtgg atcctttgtt ttcgcgttga taggcctcga tcatggcggc
       61 ccgtttttcg tcatcggcat tggccaggtc catccaccac tgcgccagga cactgtcgac
      121 ttcacgcgtt gccgcgcgca attgcagaaa gtcgacggcg gcgcgaaagc gcggctgctc
      181 aagcatgcgc cagatagttt ttgccgtggc gcgttcaaag cgcggttgca tgaaccagat
      241 ttcgcgcata tccgcctgaa aacggcgttg aatggccagt tttttagttt gttcttcgac
      301 gacggagtcg gccgcctgca tgagcgcctg gatacggggt tcaccgcttt ccatgagggc
      361 cagccagcgc ttgttgacaa gcgtccagag cagagccgca aagagaaagc ttgggcttat
      421 gggcttgccg gcgcggactc gcgtgtcggt gcgttcgaga gccagttcaa caaattgctg
      481 accgccggtt tcttcgaata tcaggtcgat cagcggcaac attccctgat gcagacccac
      541 tgcgcgcagc cgctgcaggc agtccatggc gtggccgcag gtcagcagct tgagcatttc
      601 gtcgaacagg cgcgatgctg gcacgccttc gataaggttg gccaagctct tgataggttc
      661 gcgcgtggat gggtcgagag tagcgttaag ctttgcggca aaccgcaagg cgcgcagcat
      721 gcgcaccgga tcttcgcgat agcgcgtgtt gggatctccg atgacgcgaa cgacgcgttt
      781 tttcaggtcg ccaacgccgc cgtggaaatc aatgacttgt tcttttatgg gatcgtaata
      841 cagcgcgttc aacgtaaagt cgcgccgcgc tgcatcttgt tcgtgggtgc cgaacaggtt
      901 gtcgcgcaat atgcgtccat tttcatcggt ttcctgatca gccgtcgtgg gcgcccggaa
      961 tgtcgaagtc tcgatgattt cctgtccgaa gaccacatgc acaagctgga aacgccgccc
     1021 gataatgcgg gcccgccgaa atagcggacg aatctggttg ggcgttgcgt ttgtggcgac
     1081 gtcgaaatct ttaggttcca gcccgacaat caggtcgcgc acggcgccgc ccacaatata
     1141 ggcatcgaac ccgtgttcgt gcagcacctc gcacaccttg atggcgtggc gtgaaacatt
     1201 gcgcaggtcg atgccgtgct tatcccgggt gtatcgttgc ggaccccttg ccggttttga
     1261 aaaaagccgg ctggcaaaac tttttattgt ttctgtaagc atggtgtatt ttaagacttt
     1321 gcgtccagtg tgtctttgaa aagctcaaga atgcgccaac ccctgttttc ggcaatccgg
     1381 cgtaatgccg ggctggggtt ggtggcgatc gggtgagtga cagcttccag caacgagaga
     1441 tcgttattcg agtcactata aaagtaagag cgctcgaaat ctttcagcgc catgcccatg
     1501 ctggccagcc attggttcac gcgcagcact ttcccttcct tgaagctggc agtgccgcta
     1561 atacggcccg tgtagcggcc ctcaatgtat tcgggctggg tggcgatcag atggggcaca
     1621 ccaaaggccc gggcgatggg ggccgttacg aattgattgg tggctgtgac gatggcgcac
     1681 aaatcgccct gtttcaaatg gctctgcacc aggttttggg caaccggcaa aatggcgggc
     1741 cgaatcactt ctgtcatgta gtgctcatgc cattcagcca gttcgatggc gtggccttgc
     1801 gctaataaac ccagcatgaa tgcagcggat tgctcggcgg tcaggtcgcc ggcgttgtag
     1861 cgttccatca ggtcgttatt gcgccgttgt gcttcgatgg ggtcgccggc gcggccggtt
     1921 cgcgccagaa aatcggccca ttgataatcg ctgtccagag gcaacaaggt atggtccaga
     1981 tcgaacaggg caaggcgttt ggagagagtc atgaatgtaa tggcgcggcc ggcattgggg
     2041 tcatggagag caaatctttc agtagcggca aagtgatggc ccgatgtttt tcaagcgagt
     2101 aacggtccag tgcatcgagc aaggccccga gcctgcccat atcgcgagcg tagtgcgtca
     2161 gaacccaatt tgtgatttcc ggggtgagct tgaggccctt ttcgttggcg cgcgtttcaa
     2221 atgcaacagc caaatcccca tcggacagtg gctcaaggcg aaaaaccaga tcccatccca
     2281 ggcgggtgcg cagatcttca cgcaaaggca tcgcaagcgg cgcctgatcg ccggccagca
     2341 gcaggacaaa ggcccggcgg gtcgacgata cctcacgcca gcgattgtac agtgcaaata
     2401 cggccgattg ggcgtgttca ttcaactggc cgacatcatc aatggcgatc agatcgagtg
     2461 cgacgctgtc ccccgtggcg atatctttaa gcgtgtcggc ccggtccgca acgccgaaat
     2521 acagcgcgcg cggcattgtg cctagcgctc gcagcaaatg ggtgcgcccg gcgccttgag
     2581 cgccccacaa gtaaagggcg cgacccggtt cacagctatt caatgcgtcc agtacggcct
     2641 ggtttttgcc gaccacgaag ttttcgagcg tgggcgtggg cgtcgataac aaatcaagaa
     2701 tcagttgctg ggtcattgaa tgacatcaaa gtgggggcgg tcgcagtatt gaattgtaat
     2761 agggccgggg caacgaaggc ctggccccgc ctttttcccg gataccgccc gatcatcgta
     2821 aaattggcgt tggcatttaa actcaacatg caattcttgc acatcccacg gcattttcca
     2881 tgacaaaaaa acattcagct ccgttgacct accttgatgc gggggtcgat atcgacgctg
     2941 gcgaggccct ggtcgaccgt atcaagccgc tcgccgctaa aaccatgcga gccggcgtga
     3001 tggccggcat tggcgg
//
LOCUS       DATCXU010000472         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1069561,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000472 DATCXU010000000
VERSION     DATCXU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069561"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VIR04_10520"
     CDS             complement(<1..506)
                     /locus_tag="VIR04_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY9461225.1"
                     /translation="MKLTTLDQFLGQIHARDPHQPEFMQAVKEVMHSLWPFIEKNPQY
                     AKYGLLDRLVEPERIIQFRVCWIDDQGVVQTNRGYRIQHSSAIGPYKGGMRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFNPKGKSDMEVMRFCQALITELYRHLGPD
                     TDAPAGDIG"
     gene            complement(606..1862)
                     /gene="rho"
                     /locus_tag="VIR04_10525"
     CDS             complement(606..1862)
                     /gene="rho"
                     /locus_tag="VIR04_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY9461226.1"
                     /translation="MHLTELKALHVSHLLDMAAELEIDNAGRLRKQELMFAIMKRRAK
                     QGEQIFGDGVLEVLPDGFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     TPKDGERYFALVKVDKVNDMQPEAIKHRMMFENLTPLHPDEPMILERDIRGEENVTAR
                     ILDIFAPIGKGQRALIVASPKSGKTVIMQHMAHAISANYPDAYLIVLLVDERPEEVTE
                     MQRTVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVELKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTALVETGSRMDEVIYEE
                     FKGTGNSEVHLERRLAEKRVYPAINLNKSGTRREELLIKPELLQKVWVLRKFIHDMDE
                     IEAMEFILDKIRATKSNSEFFDMMKK"
     gene            complement(1934..2260)
                     /gene="trxA"
                     /locus_tag="VIR04_10530"
     CDS             complement(1934..2260)
                     /gene="trxA"
                     /locus_tag="VIR04_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY9461227.1"
                     /translation="MSELIKHVSDASFEADVLKSGVPVLVDYWAEWCGPCKMIAPILE
                     DVSKEYQGRVIIAKVNVDENPATAAKFGIRGIPTLMLFKDGKAAATKVGALSKSQLSS
                     FLDESL"
     gene            2491..4452
                     /locus_tag="VIR04_10535"
     CDS             2491..4452
                     /locus_tag="VIR04_10535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY9461228.1"
                     /translation="MATPRYDEGSIRVLKGLEPVRQRPGMYTRTENPLHILLEVIDNA
                     ADEALAGYGKHIKVTLHADASVSIEDEGRGIPVGLHPQEKIPVAELVFTRLHAGGKFD
                     KKAGGIYAFSGGLHGVGVSVTNALSSRLEVRIWRDGRTHELVFANGDVLVPIKKLEPA
                     GRGRTGTRVRVWPDPKYFDSATVPVAELVHLLRSKAVLLNGVKVSLTIEKTGETREWQ
                     YEEGLRGYLEEALRDEEMIVPIFEGKRYAAAGDESFAQGEGAQWVVAWTADGNVLRES
                     YVNLISTTAGGTHESGLREGLFLAVRSFAEFHSLIPKGVKLLSEDVFAHASFVLSAKI
                     LDPQFQGQIKERLNSRDAVRLVSKFVENALDLWLHANVEFGKKVAESAIRQAQTRVRS
                     SKKIEKRKSSGVAVLPGKLTDCESSDATRTEIFLVEGDSAGGSAKMGRDKEFQAVLPL
                     RGKVLNSWEIERDRLFANREIYDIATAIGVDPHGPDDSADLSGLRYGRICILSDADVD
                     GSHIQVLLLTLFFRHFPKLVEAGHVYVARPPLFRMDVPALGKRPARKLYCLDQGELDA
                     AQEKLRNEGVREGAWTVSRFKGLGEMSAEQLWDTTMNPDTRRLMPVSCGALGVAATTL
                     MFDMLMGKGESAQRRTWLEEKGNLAEVDL"
     gene            4497..>5446
                     /locus_tag="VIR04_10540"
     CDS             4497..>5446
                     /locus_tag="VIR04_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY9461229.1"
                     /translation="MDSTQAGLFDGDRGDDDIMLADYAEQAYLDYAVSVVRGRALPEV
                     ADGQKPVQRRILFAMQAMGLTGNAKPVKSARVVGDVLGKYHPHGDQAAYDAMVRMAQD
                     FTLRYPLVDGHGNFGSRDGDNAAAMRYTEARLTPFSRILLDELDEGTVDFIPNYDGSQ
                     KEPDLLPARLPVMLLNGASGIAVGMATEIPSHNLRELARACVALLKKPKLSDQELFEL
                     VPGPDFAGGGQIISTAAELEHIYESGRGSIKVRARWQFEELARGQWQWVVTELPPGAS
                     SQKILEEIEDCTNPKIRAGKKSLGAEQQQTKALVLGLLDAV"
BASE COUNT         1160 a         1375 c         1659 g         1252 t
ORIGIN      
        1 ccgatgtcgc ccgccggcgc atctgtgtcc gggcccaggt ggcgatacag ttccgtgatc
       61 agagcctggc aaaaacgcat gacttccata tcggacttgc ccttggggtt gaagtccgag
      121 ccgcctttgc cgccgcccat aggcaaggtt gtcagtgcgt ttttaaatgt ttgctcgaaa
      181 gccagaaatt taagaatcga caggttcacc gacgggtgaa agcgcattcc gcccttatag
      241 gggccgatag ccgaactgtg ctgtatacgg tatccgcgat tggtttgcac gacgccctgg
      301 tcgtcgatcc agcagacccg gaattgaatg atccgttctg gttccacgag acggtcaagc
      361 agaccatatt ttgcgtactg cgggtttttc tcgataaacg gccacaaact gtgcattacc
      421 tctttgactg cctgcatgaa ttccggctgg tgcgggtcgc gggcatgaat ttggccgagg
      481 aattggtcga gtgtggttag tttcatcaag gtatgctctg atgcgtgccg agaacgcgtg
      541 tctcggcgaa gattaaaaaa atgttttgcc tttttcgggg gaaatttcgg agcggcttgc
      601 gccgtttact ttttcatcat gtcgaaaaat tctgagttgg attttgtagc gcgaatttta
      661 tccaggatga attccattgc ttcaatttca tccatgtcgt gaatgaactt gcgcagcacc
      721 caaacctttt gcagcagctc gggcttgatc agcaattctt cgcggcgtgt gcctgattta
      781 ttcagattga tggcggggta tacgcgtttc tcggccaggc ggcgttcaag gtgaacctcg
      841 gaattgccgg tgcctttgaa ttcctcgtag atgacttcat ccatgcggct gcccgtttca
      901 accagcgcag tgccgataat agtgagagag ccgccttcct ctatgttgcg cgccgcgccg
      961 aaaaagcgtt tgggccgttg cagagcattg gcgtcgacgc cgcccgtcag gactttgccc
     1021 gaggcgggaa ccacggtgtt gtaggctcga gccaggcgag tgatggagtc cagcaggatg
     1081 acgacgtctt ttttcagttc gaccagccgc ttggcttttt cgattaccat ttcggccact
     1141 tgcacgtggc gggtggcggg ttcgtcgaag gtggacgcca caacttcgcc gcgaaccgtg
     1201 cgctgcattt cggtgacttc ttcggggcgt tcatccacca gaagaacgat caggtaggcg
     1261 tctgggtaat ttgcgctgat cgcgtgggcc atgtgctgca tgatgacagt cttgcccgac
     1321 tttggactgg ccacgatcag ggcgcgctgg cctttgccta tgggcgcaaa aatgtccagg
     1381 atccgcgcgg tcacgttttc ttcgccgcga atgtcgcgct cgagaatcat cggctcgtcg
     1441 ggatgcagcg gcgtcagatt ttcgaacatc atgcgatgtt tgatggcctc gggctgcatg
     1501 tcgttgactt tatcgacctt gaccagcgcg aaatagcgtt cgccgtcctt gggtgtgcgc
     1561 acctcgcctt cgatcgagtc gcctgtgtgc aagttaaagc gccggatttg cgaaggagaa
     1621 atataaatgt cgtcggtgct ggccaggtac gatgtttcgg gcgagcgcag gaatccaaaa
     1681 ccgtccggca ggacctcgag aacgccatcg ccaaaaattt gttcgccttg tttggcgcgg
     1741 cgtttcatga tcgcaaacat tagttcttgt ttgcgcaaac ggcccgcgtt gtcgatttcc
     1801 agctcggcgg ccatgtcgag taaatgcgaa acgtgtagcg cttttagttc agtgagatgc
     1861 atgtgtggga ggaggggtgt gttggcaagg tggtggggag cccgggacgg gctcacgcgc
     1921 agggaaaaca ggtttacaag gattcgtcca gaaaggaact aagctgcgat tttgacaacg
     1981 cgccgacttt ggtggcagcc gctttgccgt ctttgaacag cattagcgtg ggtatgccgc
     2041 gaatgccgaa tttagccgct gttgcggggt tttcgtcaac gttgaccttg gctatgataa
     2101 cacgcccctg atactcttta gacacatctt cgagtatggg ggcgatcatt ttgcagggcc
     2161 cgcaccattc ggcccagtaa tcgaccagga cgggtacacc tgatttcagg acgtcggctt
     2221 caaagctcgc gtcgcttacg tgtttgatta attcgctcat aataaaagaa ggaaataggt
     2281 agtggggatt tgaaaactat acgggaacat gggctgataa cacaatgtat tctattaatt
     2341 cgttgcgtca tgttgttcta ccctgacaag cataccactg tagagcccca tgcgcacatg
     2401 gcgctacaga cgcgttttcg gccgattttc ccagtgccgg cccatcgtcc gtaaaatacg
     2461 gtttttgttc acaagagata tggaatcaac ttggctactc cacgctatga cgaaggatca
     2521 attcgggtct tgaaagggct ggagcccgtg cgccagcggc ccggcatgta cacccgcacg
     2581 gaaaatcctc tgcacatact attggaagtc atcgataacg ccgccgacga ggcgctggcc
     2641 ggctatggta agcatataaa ggtcaccctt cacgccgatg cgagcgtatc gatcgaggac
     2701 gaaggacgcg gaatacctgt tggcttgcat ccgcaggaaa aaatcccggt ggccgaattg
     2761 gtgtttaccc gcctgcatgc gggcggaaag ttcgataaga aggcgggcgg gatttacgct
     2821 ttttcagggg ggttgcacgg cgtgggcgtg tcggtgacga atgcgttgtc gtcgcggctt
     2881 gaagtccgca tttggcgcga cggccgtacc cacgagctgg tgttcgccaa cggcgacgtg
     2941 cttgtgccga taaaaaagct ggagcccgcc ggccgcggaa ggaccggaac gcgcgtccgc
     3001 gtttggcccg atccaaaata tttcgacagc gcgaccgtcc cggtggcaga actggtccac
     3061 ttgcttcgca gcaaagcggt gttgctcaat ggagtcaagg tttcgctaac cattgaaaag
     3121 acgggcgaaa cgcgcgagtg gcagtacgag gaaggtttgc gcggatacct ggaggaagcg
     3181 ctgcgcgacg aagaaatgat tgtgccgatt ttcgaaggca agcggtatgc cgccgcgggt
     3241 gacgaatctt ttgcgcaagg cgaaggggct cagtgggtgg tggcctggac ggcggacggc
     3301 aacgtattgc gggaatcata tgtcaatcta atttcgacaa ccgcgggtgg aacgcatgaa
     3361 tcgggtttgc gtgaaggctt gtttctggcg gtaaggtcgt tcgcggaatt tcatagcctg
     3421 atccccaagg gcgtcaagct gctttccgag gacgtgtttg cacatgccag tttcgtgtta
     3481 tctgccaaaa ttctggatcc gcaatttcag gggcagatta aagagcgcct gaatagtcgc
     3541 gatgcggtgc gtctggtcag taaatttgtg gaaaatgcgc ttgatttatg gttgcacgcc
     3601 aatgtcgagt tcggcaagaa agtagcggaa agcgctattc gccaggcgca aacccgggtt
     3661 cgttcatcca aaaaaattga gaaacgcaag agctcgggtg tggcggtttt acccgggaag
     3721 ttgacggact gcgaatccag cgatgccacg cgcaccgaaa tctttctggt tgaaggagac
     3781 tcggccggcg gatcggcgaa aatgggtcgc gacaaagaat tccaggcggt attgccattg
     3841 cgcggcaagg tgcttaattc ttgggagatc gagcgcgacc gcctgttcgc aaaccgcgaa
     3901 atctacgata ttgcgacggc gattggggtc gacccgcatg ggccggacga tagcgctgac
     3961 ctctcggggc tgcgctatgg ccgcatttgc atactgtcgg atgccgatgt ggatggttcc
     4021 catattcagg tgttgttgct taccctgttt ttcaggcatt ttcccaaatt ggttgaagca
     4081 ggccacgtgt acgtggcgcg gccgcccttg tttcgcatgg acgtgccagc gttgggcaag
     4141 cggcccgcgc gcaaactgta ttgcctggac cagggcgagc tggatgcggc gcaagaaaaa
     4201 ctgcgcaacg aaggcgtgcg ggaaggggcc tggactgtca gccgattcaa ggggctgggc
     4261 gaaatgagcg ccgaacaatt atgggatacc accatgaatc cggacacacg ccgtttgatg
     4321 ccggtcagtt gcggtgcgtt gggcgtggca gccaccacac tcatgttcga catgctcatg
     4381 ggaaagggcg agtccgccca gcggcgcacc tggcttgaag aaaaaggcaa tttagccgaa
     4441 gtcgaccttt gattggccgg ccacgaattc ttgagacgtt tcagagataa aaatatatgg
     4501 atagcacaca agccggcttg ttcgatgggg atcgcggcga cgatgacatc atgttggccg
     4561 attacgccga gcaagcttat ctggattacg cggtatcggt ggtgcgcggc cgcgcgctgc
     4621 ccgaggtggc cgatgggcaa aagccggtgc agcgccgcat tctgtttgcg atgcaggcca
     4681 tggggctgac gggcaacgcc aagccagtca aatccgcgcg tgtggtgggc gacgttttag
     4741 gcaagtacca tccgcatggc gaccaggctg cctacgacgc catggtgcgc atggcccagg
     4801 acttcacttt gcgctatcca ctggtggacg gtcacggcaa ctttggttcc cgcgatggcg
     4861 ataatgcggc cgccatgcga tataccgagg cacggctgac gccattctcg cgcatcttgc
     4921 tcgatgaact cgacgaaggc acggttgatt tcatacccaa ttatgacggc agccaaaaag
     4981 agccggattt gttgccggcg cgtttgccgg tcatgttatt gaatggtgct tcgggtattg
     5041 cggtgggcat ggccaccgag attccgtcgc ataacctgcg cgaacttgcc cgtgcttgcg
     5101 tggccttact caaaaagccg aaattatccg accaggaact ttttgaactg gtgccaggcc
     5161 ctgactttgc cggcggcgga cagattattt ccaccgcagc cgaactcgaa catatctacg
     5221 aatcgggaag gggctcgata aaagtacggg ctcggtggca attcgaagag ctggcccgcg
     5281 gtcagtggca atgggtggtt actgaattgc cccccggcgc ttcgtcgcaa aaaattctgg
     5341 aagagatcga agactgcact aatccgaaaa tcagggccgg aaaaaagagc cttggcgccg
     5401 agcagcaaca aaccaaggcg ctggttctgg ggttgctgga tgcggt
//
LOCUS       DATCXU010000473         3770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_758996,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000473 DATCXU010000000
VERSION     DATCXU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758996"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2212)
                     /gene="glgX"
                     /locus_tag="VIR04_10545"
     CDS             complement(104..2212)
                     /gene="glgX"
                     /locus_tag="VIR04_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY9461230.1"
                     /translation="MPSNSPDRLLPGLPYPMGATSDGLGVNFAVFSANATEVDLCLFD
                     PRGRTEVRRLTMPECTDEIWHGYLPDAGPGLLYGYRAHGPYDPKNGHRFNSQKLLIDP
                     YARQLHGAVRWSEALFGYRAQHAKSDLSLDRRDSAALVPKSVVSDDSFNWGVQNRPCT
                     PWAQTFIYEAHVRGVSKLRPGLRSETPGTCDALAHPRFIDHLQSLGVTAIELLPVHAF
                     LQDHFLVERGLRNYWGYNTLAYFAPEPAYLASGAANELRIAIRRLQAAGLEVILDVVY
                     NHTAEGNELGPTLSWRGLDNASYYRLIPGDERHYINDTGTGNTVNLSHPRVLQMVMDS
                     LRYWASSYRIDGFRFDLGVTLGREGTGFDPGSGFFDAILQDPVLSRLKLISEPWDIGP
                     DGYQLGNHPPGFAEWNDKFRDGTRRFWRGDPALRRDFAGHLMGSPEIFDRRHRRPWAS
                     INCLSSHDGFTAWDVVSYNEKRNEANGDGNADGHNENFSFNWGVEGPTDDSTVIALRE
                     RVLRAMLATVFLSDGTPMLLAGDEFGNSQQGNNNAYCQDSELSWLDWAQALSLRGRAM
                     TNFVARVHALRRAHPSLRLARFEDDNHEVIPGVMRAAWYDMDGKPMTEPAWDFTEGRV
                     IGLRRAALLPTGKAADLSFLLVNAGMEDIEFQLFEEPCRWTCELNTATGDGTAPVASD
                     AAVQVAAHSLIVLTGVVEAV"
     gene            complement(2215..>3770)
                     /gene="glgB"
                     /locus_tag="VIR04_10550"
     CDS             complement(2215..>3770)
                     /gene="glgB"
                     /locus_tag="VIR04_10550"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY9461231.1"
                     /translation="AEAAPATASVLVEPDDFKWTDDQWMATREARQRPDAAISIYETH
                     MGSWAAPDDSGSVWQATGQRLVDYVKALGFTHLELLPITVHPFGGSWGYQPLGMFAPT
                     ARWGSPGEFAQFVDLCHRAGLGLILDWVPAHFPSDAHGLVQFDGTALYEHADPKQGLH
                     PDWNTLIYNMGRNEVRNFLIASALEWLRRFHVDGLRMDAVASMLYLDYSRKPGQWTPN
                     QYGGRENLEAVEFIRQVNAVVAEHCPGAIMIAEESTAWPGVTSPQEQGGLGFQYKWNM
                     GWMHDTLRYMQHEPVYRRYHHHDMTFGLVYAWSERFILPLSHDEVVHGKRSLLGKMPG
                     DRWQRFANLRAYYGFMWGHPGKKLIFMGGELAQEHEWNHDAQLDWPALRDPMHSGVQS
                     LVRDLNALYADLPALYGSDAVPAGFQWVIGDDQNNSVFAFLRLYGTCVALVVCNMTPV
                     PRIAYRIGVPRAGRWIERLNTDAAMYGGSNMGNAGSSPSQELPAHGQPYSLSLVLPPL
                     ATLIFSAEQ"
BASE COUNT          852 a         1136 c         1037 g          745 t
ORIGIN      
        1 ggcaatgcgc cgaatgtata ttcaaacggg ccgctgaaac gttcatgcgg atcgagcgcg
       61 gacgcagggg aaggaaacgg ggaatagaaa gttcgagttt gcattaaacc gcctccacaa
      121 cgcccgtaag cacgatcaga ctatgcgcag ccacctggac cgcggcatcc gatgcaaccg
      181 gggcagtacc atcgccagtc gccgtgttta attcacatgt ccagcggcat ggctcttcaa
      241 ataactgaaa ctcaatatct tccatgccgg catttaccag cagaaacgag agatcagcag
      301 ctttgcccgt tggcaacaac gcagcacgcc tcaggccgat gacacgccct tccgtgaaat
      361 cccacgcagg ttcggtcatt ggctttccgt ccatgtcgta ccaggctgcg cgcatgactc
      421 ccggtatgac ctcgtggttg tcgtcttcaa atcgcgccag cctcaatgaa ggatgagcac
      481 ggcgcaatgc atggacacga gccacgaaat tcgtcatggc ccggcctcgc aacgagagcg
      541 cctgagccca gtccagccat gacaactcgc tgtcctgaca gtaagcattg ttattgcctt
      601 gttggctgtt gccgaattcg tctccggcca atagcatggg ggtgccgtcg gataaaaaca
      661 cagtagctag cattgctcgc aatacccgtt cgcgcaacgc gatgacggtc gaatcatcgg
      721 tagggccctc tacgccccag ttgaagctga agttttcgtt atgtccatcg gcgtttccat
      781 cgccattggc ttcgttgcgt ttttcgttat agctgaccac gtcccaggca gtgaatccgt
      841 cgtgcgacga caagcagttt atcgaagccc acgggcggcg atggcgccgg tcgaatattt
      901 ccggcgaacc catcaagtgg cccgcaaaat cgcgccgcag cgccgggtcg ccccgccaga
      961 aacgtcgcgt gccgtcgcga aatttgtcgt tccattccgc aaagcccgga gggtgattcc
     1021 ctaattgata accgtctggc ccgatatccc aaggttccga aatgagcttc agccgggaaa
     1081 gaacaggatc ctgcaggatc gcatcaaaaa aaccagagcc cggatcgaag ccagtccctt
     1141 cgcggcccag cgtcacgccc aaatcgaagc gaaagccatc aatgcgataa ctgctcgccc
     1201 aatagcgcaa agaatccata accatttgca gcacgcgcgg atgcgacaga tttacggtat
     1261 tgccggtccc cgtgtcatta atgtaatggc gttcgtcacc cggaatcagt cggtaatagc
     1321 tggcattgtc caggcctctc catgacagcg tgggtcccag ttcgttgccc tcggccgtat
     1381 ggttgtagac gacatcgagt atcacctcca ggccagccgc ctgcaggcga cggatggcaa
     1441 tgcgcaattc gttggccgca ccgctggcca gataagccgg ttcgggtgcg aaataggcca
     1501 gcgtgttata gccccaataa ttgcgcaaac cgcgttcaac cagaaaatgg tcttgcaaaa
     1561 aggcgtgcac tggcagcaat tcaatagccg tgacgcccag gctttgcaaa tggtcaatga
     1621 atcgggggtg agccagcgcg tcgcatgtgc cgggcgtctc ggatcgcaag cccgggcgca
     1681 acttcgacac cccacgcacg tgcgcctcgt aaataaatgt ttgagcccac ggagtgcaag
     1741 gccgattttg tacgccccaa ttaaagctgt cgtcgctcac caccgacttg ggcacgagcg
     1801 ccgcactatc gcgccggtcc agactaagat ctgattttgc gtgctgcgcc ctataaccga
     1861 acagcgcctc ggaccagcgt acggctccat gcaattgccg cgcataaggg tcaatcagca
     1921 gtttttgcga attgaaccgg tggccgtttt tcggatcgta cggcccatga gcgcggtaac
     1981 cgtacagcaa cccggggccc gcatcgggca gataaccgtg ccagatttcg tcggtgcatt
     2041 cgggcattgt caggcgacgc acttcagtcc ggccgcgtgg gtcaaaaaga cataggtcga
     2101 cttcggtggc attggccgaa aatacggcga aatttacgcc caacccgtcg ctggtagcac
     2161 ccatgggata cggaagtccg ggcaaaagcc ggtcgggcga gttcgatggc atagtcattg
     2221 ctccgcagag aatataagcg ttgccagtgg cggaagcacc agcgatagcg agtacggttg
     2281 gccatgagcg ggcaattcct gcgacgggga cgatccggca ttgcccatat tggagccgcc
     2341 atacattgcc gcatctgtgt tcagacgctc gatccagcgg ccggcccgcg gcacaccgat
     2401 tcgataagca atgcgcggga ccggcgtcat gttgcacacc accagcgcga cgcatgttcc
     2461 ataaagacgc aaaaaggcaa agacgctatt attttgatcg tcgccgatca cccactgaaa
     2521 gccggccggg acggcatcgc tgccatacag cgccggcaag tctgcataca gcgcattcaa
     2581 atcgcgcacg aggctctgca cgcccgaatg catcgggtcg cgcagggcgg gccaatccag
     2641 ttgcgcgtcg tgattccatt cgtgctcttg agcgagctcg ccacccatga aaatcagctt
     2701 ctttcccgga tgaccccaca taaatccata ataggcccga agattggcga agcgctgcca
     2761 gcggtcgccc ggcatcttgc ccaacaacga cctcttgcca tgcaccacct cgtcatgcga
     2821 caagggaaga atgaaccgtt cggaccaggc gtagaccaga ccgaaggtca tatcgtgatg
     2881 atggtaacgg cgatacactg gctcgtgttg catgtaacgc aaggtgtcgt gcatccagcc
     2941 catgttccat ttatattgaa atccaagacc gccctgctct tgcggcgacg taacccctgg
     3001 ccaggcggtc gactcctcgg cgatcataat cgcgcccggg caatgttcgg caacgacagc
     3061 atttacctgc cgaatgaact cgaccgcctc caggttttcc cggccgccat attgattcgg
     3121 tgtccactgg ccgggcttgc ggctgtagtc cagatacagc attgacgcca ccgcatccat
     3181 ccgcaggcca tcgacgtgaa aacgtcttaa ccattcaagt gcgctggcga tcagaaaatt
     3241 gcggacttca ttgcgcccca tgttatagat caatgtattc cagtcgggat gcaggccttg
     3301 tttaggatct gcatgttcgt acagcgctgt accatcgaat tgaaccagcc cgtgcgcatc
     3361 tgaaggaaaa tgtgctggca cccaatccag gataagcccc agcccggccc ggtggcacaa
     3421 atcgacgaat tgcgcgaact cgcctgggga gccccaacga gcagttggcg cgaacattcc
     3481 caacggctga tatccccagg agccgccaaa cggatgcacg gttatcggca atagctccaa
     3541 atgcgtgaat cccagcgcct tgacgtagtc caccagacgt tggccggttg cctgccagac
     3601 gctgcctgag tcgtcaggcg cagcccatga acccatgtgg gtttcgtaga tcgagatagc
     3661 ggcgtcaggt cgttgccgcg cctcgcgcgt cgccatccat tggtcgtcgg tccatttaaa
     3721 atcatcgggt tcgacgagta ccgacgcggt ggcgggcgcc gcctcggcct
//
LOCUS       DATCXU010000474         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_730528,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000474 DATCXU010000000
VERSION     DATCXU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730528"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIR04_10555"
     CDS             <1..322
                     /locus_tag="VIR04_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY9461232.1"
                     /translation="AAFAEWAGARLPTEFEWEAAALRPGIAQAQGFAWQWTRSSYDPY
                     PGYHPQAGAIGEYNGKFMVGQLVLRGSSCATPAGHARNTYRNFFPPAARWQFSGVRLA
                     KDMA"
     gene            406..1308
                     /gene="egtD"
                     /locus_tag="VIR04_10560"
     CDS             406..1308
                     /gene="egtD"
                     /locus_tag="VIR04_10560"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY9461233.1"
                     /translation="MLASLQVRPRSLSPKYFYDEKGSALFDRICDLPEYYPTRTELAL
                     LAAHAEEIAMKMGPRAEIVEFGAGSLRKVRLLLDVMDEPSRYVPIDISGVYLSASAAQ
                     LQRDYPSLSVSPIMADYTAGVHLPASEPRSGCRVGFFPGSTIGNLHPADALKFLRQAA
                     QSLRGGALLIGVDLIKAPSILHAAYNDSQGITAAFNLNLLARANRELDADFVLSQFVH
                     GAFYNAPLQRVEMHLISRTAQQVHVCGECFDFEEGETLHTENSYKFTVNSMRAMAVRA
                     GFQPARVWIDPQKLFSLHWLRAPL"
     gene            1405..1962
                     /locus_tag="VIR04_10565"
     CDS             1405..1962
                     /locus_tag="VIR04_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY9461234.1"
                     /translation="MANQRKEQPAHEGFVMDVKAIRARARENLDRGAVTEGYTADRST
                     VLKLLNDALATELVCVLRYKRHQYMASGIHAEPVAAEFAAHAAEEQGHADMIAARIIE
                     LGGAPDFAPDSLSGRSHSEYVEGANLKDMIKENLVAERIAVDSYREIIVYLGDRDPTT
                     RRMMEEILAVEEEHAQDMADLLERD"
     gene            complement(1971..2396)
                     /locus_tag="VIR04_10570"
     CDS             complement(1971..2396)
                     /locus_tag="VIR04_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY9461235.1"
                     /translation="MKAILVPVDGSDCSTRALKFAIEVAKGRDDVALHVLTVQAPIIS
                     GNVKRFVSTEAINSYYQEEGEKALKPARSLLDEAGISCKGIIEVGPAARTIAEFVKRH
                     DCDVIVMGTRGLGAVANLVLGSITTKVLHLVDVPVTLVK"
     gene            2457..>2885
                     /locus_tag="VIR04_10575"
     CDS             2457..>2885
                     /locus_tag="VIR04_10575"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY9461236.1"
                     /translation="MKAKIEDYALLGNCRTAALVSNRGSIDWLCFPRFDSPSCFTALL
                     GKAHNGRWKIAPSSSITSVKRSYRDDTLILETTFTTEQGEATLIDFMPATFSHSSVVR
                     IVVGVKGCVSFDMDLVIRCDYGRSIPWVEHPEPHTLTAVAG"
BASE COUNT          624 a          791 c          809 g          661 t
ORIGIN      
        1 cgcggccttt gcagaatggg ccggcgcgcg tttgcctacg gaattcgaat gggaagcggc
       61 cgcgctcagg cccggcatcg cccaggcaca gggctttgca tggcagtgga cgcgttcgtc
      121 ttacgaccca taccccggct accacccgca agcgggggcc atcggagaat ataacggaaa
      181 gttcatggtg ggccagttag tgctgcgcgg cagtagttgc gccactccgg ccggccatgc
      241 acgcaatacc tatcgaaatt tttttccgcc cgccgcgcgt tggcaatttt cgggtgtgcg
      301 tctggccaag gacatggcat gacaattacc cagcagcgtc gtcgagataa cgattttctt
      361 aacgtggcca ataaaaggcc tccctcgggg gatttcgccg gcgatatgct cgcctcgctt
      421 caagtgcggc cgcgcagcct gtcgccaaag tatttctacg acgaaaaggg ttccgcgttg
      481 tttgatcgca tttgcgactt gcccgagtat tatccaacgc gcacagaatt ggcgttgttg
      541 gccgctcatg cagaggaaat cgccatgaaa atggggccgc gcgccgaaat cgtcgagttc
      601 ggagcaggct cgttgcgaaa ggtccgtttg ttgcttgatg ttatggacga accctcgcgt
      661 tacgtgccaa ttgatatctc cggagtttat ctgtcagcca gtgccgctca attgcagcgc
      721 gactacccga gtttaagcgt gtcgccgatt atggctgatt acactgccgg ggttcatttg
      781 ccggcaagtg aaccccgctc gggctgccgg gtgggcttct ttccgggctc cacgatcggc
      841 aatctgcatc cggccgacgc tttaaaattt ctgcgacaag cggcacagtc attgcgaggc
      901 ggcgccttgc tcataggcgt ggatctcatc aaggcgccat cgattttgca tgccgcttat
      961 aacgactccc aaggcattac cgcggcattc aatttgaatt tgctggcgcg cgcaaatcgc
     1021 gaactcgatg ccgacttcgt cctttcacaa ttcgttcacg gcgcgtttta taacgcgcct
     1081 ttgcagcgtg tcgaaatgca tttgataagc cgtacggcgc aacaggtcca cgtgtgcggg
     1141 gagtgtttcg acttcgagga gggcgaaact ttgcacaccg aaaattcgta caaattcacg
     1201 gtcaacagca tgcgcgcaat ggctgtgcgg gccggattcc agcccgcacg agtgtggatt
     1261 gatccgcaga agctgtttag cctgcattgg ctgcgggcgc cgctttaaca gaacgagcat
     1321 gtagtatatt gactaacgct tgtctctcgg acgggatcag cctggttttg cgtcgggaga
     1381 caactttcca caggagcaaa ttacatggct aaccaaagaa aggaacaacc tgctcacgaa
     1441 ggctttgtta tggacgtcaa agccatccgg gcccgggcgc gcgaaaacct ggatcgcgga
     1501 gcagtgactg aaggttatac cgccgaccgg agcaccgtgc ttaaactgct taacgatgcg
     1561 ctagccaccg aattggtttg tgtgctgcgt tataaacgcc atcagtacat ggccagcggc
     1621 attcatgcgg aacctgtagc cgccgaattt gccgcgcatg ccgccgaaga gcaaggccac
     1681 gccgacatga ttgcggcccg cattatcgaa ttgggcggag cccccgactt cgcacccgac
     1741 agcctttccg ggcgtagtca ttccgaatat gtggaaggcg ccaatctgaa agacatgatc
     1801 aaggaaaacc tggtggccga gagaatcgcg gtagacagct accgcgagat aatcgtctat
     1861 ctgggcgacc gcgacccaac gacgcggcgc atgatggaag agattctcgc ggttgaagaa
     1921 gagcacgccc aagacatggc cgatttgctc gagcgcgact aggcggtctg ttatttgacc
     1981 agggtaaccg ggacgtcgac caggtgcaaa actttggtgg tgatggaacc cagcaccagg
     2041 ttggcgaccg ctcccaggcc ccgggttccc ataacgatca cgtcacagtc atgccttttc
     2101 acgaactctg cgattgttct tgcggcgggg cctacttcaa ttattccctt gcaagaaata
     2161 ccggcctcgt caagtagcga cctggcgggt tttaacgcct tttcgccttc ttcctggtaa
     2221 tagctgttta tggcttctgt tgaaacgaac cgtttgacgt tgcccgaaat tatcggagct
     2281 tgcaccgtca gcacatgcaa agctacatcg tcgcgtccct ttgcgacttc aatggcgaat
     2341 ttcagggcgc gggtggaaca atccgatccg tccacgggca caaggattgc cttcatcata
     2401 cgtatccttt ggaagttcag tcgatagatt acaacatatt ggcggagtaa aagctgatga
     2461 aagccaagat cgaagattac gctttgctgg ggaattgccg cactgcggct cttgtttcga
     2521 accgcggctc gatagattgg ctttgctttc ctcgctttga ctcgccatca tgcttcaccg
     2581 ccttgttggg aaaagcccat aacggacgct ggaagatcgc gccttcgtca tcgataacaa
     2641 gcgtcaagcg cagctaccgc gacgatacgc tgattcttga aaccaccttt acaaccgaac
     2701 agggcgaagc gaccctgatc gatttcatgc cggccacttt cagtcactcc agtgtcgtgc
     2761 gcattgtcgt gggcgtaaaa ggatgcgtgt catttgatat ggatctggtc atacggtgtg
     2821 attatggtcg ttctattccg tgggtcgagc atcctgaacc gcatacgctg actgctgtgg
     2881 cgggg
//
LOCUS       DATCXU010000475         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_601839,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000475 DATCXU010000000
VERSION     DATCXU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601839"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2199
                     /locus_tag="VIR04_10580"
     CDS             <1..2199
                     /locus_tag="VIR04_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY9461237.1"
                     /translation="ASDPAWSHSVHGVATANQGGTFGAYISDLLLFLFGYSAWLWVVL
                     LAQRVAVGFYRLANVLTSNRKIEPLPRVHWEVGIGFFLLFIGTMGTEALRLKQFGQQL
                     PGGAGGELGKLLARLMSLSLGTTGCTIVLLAFIAIGASLFFGFSWLHLSERVGLFIEQ
                     SVRRVFEFKAAHDDRKVGEAKSAERDEIVVARQERFVHEQPVRIEPAVTSVKRSARAE
                     QEKQKTLFTAPVDRGGSGDLPAIGLLDMPVENPETVAPETIEFTSRLIEKKLSDFGVN
                     VVVVAAQAGPVITRYEIEPAVGVKGSQVVNLAKDLARALSLVSVRVVETIPGKNLMGL
                     ELPNPRRQMVTLSEIIGSQVYHANPSVLTMALGKDIAGNPIVADLAKMPHLLVAGTTG
                     SGKSVGINAMILSLLYKADATQVRLILIDPKMLEMSIYEGIPHLLAPVVTDMRHAANA
                     LNWCIGEMEKRYRLMSKMGVRNLAGYNAKIREAIKREEPIANPFSLTPDAPEPLVALP
                     AIVVIIDELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRISFQVSSKIDSRTILDQMGAEALLGQGDMLYMPPGTGFPVRVHGAFVHDDEVHRV
                     VDYLKQQGEPNYVDGLLEGALEGETGDGLGGVTGFADAESDPLYDQAVAIILKNRRAS
                     ISSVQRHLRIGYNRAARLLEQMEQAGLVSAMQSNGNREILVPAGNTSNAE"
BASE COUNT          469 a          628 c          714 g          524 t
ORIGIN      
        1 gcttcagatc ccgcttggtc gcattcggtg cacggcgtcg cgacggccaa tcagggcggc
       61 accttcggcg cgtacatttc cgacttgctg ttattcttgt ttggctattc ggcctggctt
      121 tgggttgtgc tgctggcgca gcgcgtggcg gtgggatttt accgcctggc caacgtcctc
      181 acatccaatc gcaaaatcga gcccctgccg cgcgtgcatt gggaagtcgg tattggcttc
      241 tttctgcttt ttatcggcac aatgggcact gaagcccttc ggttgaaaca attcggtcag
      301 caactgccgg gcggagcggg cggcgaattg ggcaagctgc tggctcggct gatgtcatta
      361 tccctgggca cgaccggttg cacgatcgtg ctgttggcgt tcattgccat tggggccagt
      421 ctgtttttcg gtttttcctg gctgcatctt tccgagaggg tcggattgtt tatcgagcag
      481 tcggtgcgcc gcgtgttcga gttcaaggcg gctcacgatg atcgtaaagt gggcgaggcc
      541 aaaagcgcgg agcgcgatga gatcgttgtc gccaggcaag agcggtttgt gcatgaacaa
      601 ccggtacgca tcgagccggc cgtcacgtcc gtgaaaagat ccgcccgggc tgaacaagag
      661 aaacagaaaa cattgttcac cgctcccgtc gatcgcggcg gatcgggcga cctgccggca
      721 attggtctgc tggatatgcc ggtcgaaaac cctgaaacag tcgcgcccga aacaatcgag
      781 ttcacatcgc gtctgattga gaaaaagctt tccgatttcg gcgtcaacgt ggtggtggtg
      841 gcggctcagg ccgggccggt catcacgcgc tacgaaattg aaccggctgt tggcgtcaag
      901 ggtagccagg ttgtcaattt ggcaaaagat ctggcgcgtg cgttaagcct ggtcagcgtc
      961 cgtgtggtgg aaacgattcc cggcaagaat ctgatggggc tggaattgcc caatccccgg
     1021 cgccagatgg tcactctgtc cgagatcatt ggatcgcagg tctaccatgc caacccgtcg
     1081 gtattgacca tggcgctggg taaagacatt gccggcaatc ccattgtggc cgatctggcc
     1141 aagatgccgc atcttctggt ggccggcacg acgggttcgg gcaagtcggt aggcatcaac
     1201 gccatgattt tatctttgct gtacaaggcc gacgcgactc aggtgcgctt gatcttgatt
     1261 gatccgaaga tgctcgaaat gagcatctac gaaggtattc cgcatttgtt ggcgccggtg
     1321 gtgaccgata tgcgccatgc cgccaacgcg cttaactggt gcatcggcga aatggaaaag
     1381 cgttaccgtc tcatgagcaa aatgggcgtg cgcaatctcg cgggctataa cgccaaaata
     1441 cgcgaagcca ttaagcgcga agaacctatc gccaatcctt tttcgctcac gcccgacgcg
     1501 cccgaaccgc tggttgcact gcccgccatt gtggtcatta ttgacgagct tgccgatctg
     1561 atgatggttg taggcaaaaa aattgaagag ctcattgccc gcctggccca gaaggcgcgt
     1621 gcggcaggaa ttcatcttat cttggcgact cagcgaccca gcgttgatgt aattaccggt
     1681 ctgatcaagg ccaacattcc cacgcgcatt tcgtttcagg tgtcgtccaa aatcgactcg
     1741 cgcaccattc tcgatcagat gggcgccgaa gctctgctgg gtcagggcga catgctttat
     1801 atgccgccgg gcacgggatt tccggtacgg gtacacgggg catttgtgca cgacgacgag
     1861 gtgcatcggg tggtcgacta cttgaaacag cagggcgagc ccaactatgt cgatgggctg
     1921 cttgaaggcg cgctggaagg cgaaacagga gatgggttgg gcggtgtcac cggttttgcc
     1981 gacgcggaat ccgacccgct gtatgatcag gcagtggcga tcatattgaa aaatcgacgg
     2041 gcgtctattt catcggtgca gcggcatctg cgcatagggt acaaccgcgc ggcgcgcctg
     2101 ctcgaacaaa tggaacaggc tggtctggtg tcggccatgc agtccaacgg caatcgggag
     2161 attctggtcc cggcgggaaa cacctccaat gcggaataaa gtctttttgg cctctctgtt
     2221 gctgtcgatg gctcctttga tcgcaacggc ggccgatgcg cagaaccagt tgcggcaatt
     2281 tgtggcgacg gtggcgtcgg ccactggcaa cttcacgcag caaacttcgc ccgct
//
LOCUS       DATCXU010000476         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_528035,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000476 DATCXU010000000
VERSION     DATCXU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528035"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..543)
                     /locus_tag="VIR04_10585"
     tRNA            complement(455..543)
                     /locus_tag="VIR04_10585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(507..509),aa:Leu,seq:taa)
     gene            665..1201
                     /locus_tag="VIR04_10590"
     CDS             665..1201
                     /locus_tag="VIR04_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY9461238.1"
                     /translation="MKLPAALVLVLAALCVPAMAQTTHPKPTPEDLRPNNPKVPDAYA
                     ISGHFTRIVVMRMKYRTDLLRGMEKLVKEQKIRNGVILSGIGSLRGYRVHSVSSRDLP
                     PSDSFVAAPNAPANLNGMNGYIVDGDIHAHVVMAVNQGRDTVSGHLEAGCEVLTYAIV
                     TVGVLDTPLGRVQEQNYR"
     gene            1284..1676
                     /locus_tag="VIR04_10595"
     CDS             1284..1676
                     /locus_tag="VIR04_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY9461239.1"
                     /translation="MRKPVLMALAMIAASSAAWAEPVTPAHHALFSQTGRASYYASAL
                     EGNKTADGSIFDGDAFTAAHRSLPFGTVVRVTNLATGRMVKVRVNDRGPFTRRMVIDL
                     SEAAARELGMFRRGIARVTLKVLKADQP"
     gene            1795..2085
                     /locus_tag="VIR04_10600"
     CDS             1795..2085
                     /locus_tag="VIR04_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461240.1"
                     /translation="MNRQSVNRVCTAVPLILSLMACVWVLGNVAGGIRSGNSDEGLGF
                     KVFWLLILAQVPFGLGYLATADWNRLSKVATSIVLQVAALVLAFSPVAYFKL"
     gene            complement(3017..3214)
                     /locus_tag="VIR04_10605"
     CDS             complement(3017..3214)
                     /locus_tag="VIR04_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461241.1"
                     /translation="MATVYSGKRPNLVPGQETFNSQHMGTAEYLRSIRAEITPGSEQI
                     VDDKLVDGQGRYVGADGKTDA"
     gene            3437..4051
                     /gene="rpsD"
                     /locus_tag="VIR04_10610"
     CDS             3437..4051
                     /gene="rpsD"
                     /locus_tag="VIR04_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY9461242.1"
                     /translation="MSKRANSKYKLDRRVGENLWGRPKSPVNKRSYGPGQHGQRRAKK
                     LSDYGTQLQSKQKLKGYYGNITERQFRKYYQDAARRPGDTGENMIGLLETRLDTVVYR
                     SKFVPTPFAARQLVSHGHVKVNGRRVTIASYHVKEGDTLELTAKARDMALVLEAAKSA
                     ERDTPDYLEVAEAKSSKLLRTPKLADVPYAVQMEPHLIVEFYSR"
BASE COUNT          802 a         1315 c         1232 g          899 t
ORIGIN      
        1 ctgccgcaag gcgggtgtga aggccggcat cttctgcggc aaccccgacg acgcgcttgt
       61 catgatcggg cgcggcttcg atttcgtcac cgccgtgatc gatgacgcct tgctggcggc
      121 tggcgccgac atccggcggc ggttttcctg agcgttcgcg gcgcatattt caaaccgtta
      181 cggccgcttc tgtgctacaa ggcacacgca acaatgcttt gaaaggaggt ggtccagtgt
      241 ctcattgtca tcttatggcc gcgagctgga tcctcgtttc gaccggggtc cgcgtctaaa
      301 gcgcctcgcg ttccagcgaa gacaatggaa gggccgtgcg ccaggcgtgc ggcccttctt
      361 tgtttcggcg ggtcttttgg gattgtaact cagtgctgcc gcacggctgg acgccgcttg
      421 gcattcaaat gcgactttgc gggtcgcgtt tgaatggtgg ccccggtccg agtcgaacgg
      481 acacgtccct ttcggaacaa cggattttaa gtccggtgcg tctaccagtt ccgccacggg
      541 gccgcccggc gctcagttag ccgaggtgga gcgggttctg caaacccgcc gcgcgcggca
      601 aggtcgcctc ggccccaccg gcacgctagg atggccggcg gcgtcccgac gtgaggagat
      661 cgtgatgaag ctgcctgccg cgcttgtcct tgtgcttgcc gccctttgcg tgcccgccat
      721 ggcgcagacc acccatccca agccgacgcc ggaagacctg cgtcccaaca atccgaaggt
      781 gccagacgcc tatgccatca gtggccattt cacccgcatc gtggtgatgc ggatgaaata
      841 ccgcaccgac ctgctgcgcg gcatggaaaa gctggtgaag gaacaaaaga ttcgcaacgg
      901 cgtcattctg tcgggtatcg gctcgctgcg cggctatcgc gtccacagtg tttccagccg
      961 cgacttgccg ccgtcggaca gttttgtggc ggcgcccaac gcgcccgcca acctgaacgg
     1021 gatgaacggc tatatcgtcg atggcgacat tcacgctcat gtcgtcatgg cggtgaatca
     1081 gggccgcgat acggtctccg gccatcttga ggccggctgc gaagtgctga cttatgccat
     1141 cgtcacggtg ggcgtgctcg atacgccact gggccgggtg caggagcaga attatcgcta
     1201 gcgggcttca cggaactttt gttccggggt tgagcgtttc gcgatccggc ggttaagctg
     1261 acggcgggga tacgcggaca gcgatgcgaa aaccggttct catggccttg gcgatgatcg
     1321 ctgcctcttc agctgcgtgg gcggagcccg tcacgccggc gcatcatgcg ctgttcagcc
     1381 agacggggcg ggcctcctat tacgcttcgg cgctggaagg aaacaagacc gcagacggtt
     1441 cgatcttcga cggcgatgcc ttcaccgccg cgcatcgttc gttgcccttc ggcacggtgg
     1501 tgcgcgtgac aaacctggcc accggacgga tggtgaaagt gcgggtcaac gaccgtgggc
     1561 cgttcacgcg gcgcatggtg atcgacctgt cggaggcggc ggcgcgcgag ttgggcatgt
     1621 tccgccgcgg catcgccagg gtgacgctga aggtcctcaa ggccgaccag ccctgagcat
     1681 cttaggatgc gcctgagcgc gcgttgccga tcacgtcagc cttgcgcttc tcgccggatg
     1741 gtctcttagg catggcgcgc gtatgctcct aactctgaaa acgtggaggt ctccatgaac
     1801 cgccaaagcg taaaccgcgt ttgtaccgcc gtcccgctta tcctatccct catggcttgc
     1861 gtctgggttc tcggcaacgt cgccgggggc attcggtctg gcaacagtga cgaagggctt
     1921 gggttcaaag tcttctggct cctgatcctg gcccaggtgc cttttggcct gggatatctg
     1981 gcaactgccg actggaaccg cctgtccaaa gtggccacca gcattgtcct acaggtcgcc
     2041 gcccttgtct tggccttctc gccggtagcc tatttcaaac tgtaacagtg cccaatgcgg
     2101 gcactggttc agtttgactt ggggttgcaa ggccaagttt ctctaagggc gagaaccacc
     2161 cctatgagtc gattctcgct ccagcgttcg ggatgctttt gcgcccaatt ggtaaaggct
     2221 tgggcggcgg ctccatatga aatatccccg caaatgcttt gcttgggatt agtccccgat
     2281 agccccatgc ctttcaggta gtcgccgatt ccgcccacat accccaagca atacgtggca
     2341 ttggggtcta agtctggatg taacccggaa agccctctgc actcctgaag aagaccttgg
     2401 atagtcattg tcgcgtcggg agtggggtcg gcagcaaatg cattggctgg ccataacgcc
     2461 aatagaagaa atagttcagc cttacgcagc gaccacttca tttttcgcca tctccgctgc
     2521 ttcgatcagc ttcacgatat cgccaatatc ccatatacgt ttgaagttcg tctcttggga
     2581 ccgaacgatg caattcttac ccaggccaca atggaattta agttctccaa taataagccg
     2641 agcatcctcg ttctcaatca gatgcctatt caatttcaga ctgtgggcac atttgatttc
     2701 caaatgaagt ctggaggcga cgactgggag acgctcaaat cgttcgaaat tcgcgagcgc
     2761 gcccctcgac cgtcgccgcc gactacgccc agctagttac tttttccagc gagtagctgc
     2821 agccttctta gcgatctctg cgcgacgctc cggcgtcata gactccgcgc gagccttgcc
     2881 gcccttgaga ccgcccttgc ggtttgcctc agtgccggga ttgtgaccgc tttccttctc
     2941 ctcccccgtc gcaattcgca tgatctggac ggcgcgggat atggcgtcgg cggggcgctt
     3001 ctggccggag ggtcccttag gcatcggttt taccgtccgc ccccacgtag cgcccctgcc
     3061 cgtccactag cttgtcatcc acaatctgct cggaaccagg ggtaatttcg gcacggatgg
     3121 agcgcaaata ttccgccgtt cccatatgct gggaattgaa ggtttcttgt cccggcacaa
     3181 gattcgggcg ctttccggaa tacacggtag ccatcgaggc accctagccc ccggcccagc
     3241 ccccttcaat cccggctatt tttcaaactg taccagtgcc cccaatgcgg gtggcgcacg
     3301 gcataagtga ttgatttcaa acaggttgca aaggcatact aaacctatgg ttgcatatgt
     3361 ttgactcggc taaagccttc cgtataagtg cgcgccttgc cggcatcccc ggacccgaaa
     3421 ttaccgaaag agaacgatga gcaagcgcgc caattccaag tacaagctcg accgccgcgt
     3481 cggcgaaaat ctctggggcc gtcccaagag ccccgtcaac aagcgctcct atggccccgg
     3541 ccagcatggc cagcgccgcg ccaagaagct gagcgactac ggcacgcagc ttcagtccaa
     3601 gcagaagctg aagggctatt acggtaacat caccgagcgg cagttccgca aatattacca
     3661 ggacgccgcc cgccgccccg gcgacaccgg cgagaacatg atcggcctgc tcgagacccg
     3721 tctcgacacc gtggtctatc gctccaagtt cgtgccgacg ccgttcgccg cccgtcagct
     3781 ggtcagccat ggtcatgtca aggtcaatgg ccgccgcgtc accatcgcct cctatcacgt
     3841 caaggaaggc gacacgctgg aactgaccgc caaggcgcgc gacatggcgc tggtgctgga
     3901 agccgccaag tcggccgaac gcgacacccc cgattatctg gaagtggccg aggccaagtc
     3961 gtccaagctg ctgcgcaccc ccaagctggc cgacgtgcct tacgccgtgc agatggaacc
     4021 gcacctgatc gtcgagttct attcgcgtta attcgacggc acgaaatgca aaaagcccgg
     4081 tcgcaagacc gggctttttt gttctctcac catgggcggg ttcaatccgc ccatccaact
     4141 ttttaccatt tgatgaaagc tggatggccg gctcaaggcc ggccatggag aataatgact
     4201 aggcgtcggc ggtggccacg cccttttcgg ccagtaaagt cttcagtt
//
LOCUS       DATCXU010000477         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_604327,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000477 DATCXU010000000
VERSION     DATCXU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604327"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIR04_10615"
     CDS             <1..513
                     /locus_tag="VIR04_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9461243.1"
                     /translation="GLPSHQLAHAGINRTFQIPKPFRTLTVRENVQVAATYGRRGTSS
                     DEIAAVLDLLDLTDQAGRKAEELNSVHLKMLDLARALATKPVLLCLDELAAGFNPGEL
                     THVVDQVHSIARSGVAIIVVEHLMGFIEDVTDRVIVLNAGKGIFEGLLSEAAANRQVI
                     DVFLGDAHAV"
     gene            503..1234
                     /locus_tag="VIR04_10620"
     CDS             503..1234
                     /locus_tag="VIR04_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461244.1"
                     /translation="MPSELFSARDIHAGYGRVQVLWGAGLSVNEAQTVVLLGMNGAGK
                     TTLIKTIMGLIHQSKGTITFNGADLSHTRTDKRVRAGISYMSEVGCFPGLSVDENIRI
                     GGQFMLPSALHQRIGELYEIFPAIAARKNALASSLSGGQRKMVGIAKALAGNPKLLIM
                     DEPSAGLSPLLVQEVIEVLARFRKEGISFLIAEQNVRFLELADHVVTMSGGRMGFSGT
                     VEAMRADDALRKAYFDIKGEHSNAG"
     gene            1327..1402
                     /locus_tag="VIR04_10625"
     tRNA            1327..1402
                     /locus_tag="VIR04_10625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1360..1362,aa:Ala,seq:ggc)
     gene            1455..1530
                     /locus_tag="VIR04_10630"
     tRNA            1455..1530
                     /locus_tag="VIR04_10630"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Glu,seq:ttc)
     gene            1576..1652
                     /locus_tag="VIR04_10635"
     tRNA            1576..1652
                     /locus_tag="VIR04_10635"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1610..1612,aa:Asp,seq:gtc)
     gene            complement(2115..2396)
                     /locus_tag="VIR04_10640"
     CDS             complement(2115..2396)
                     /locus_tag="VIR04_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY9461245.1"
                     /translation="MSNQRFASIWDAIEDTQEEAENMKLRSVLMTALKQHIIRVKMSQ
                     TEAAKLLGVTQPRISDLMRGKINVFGLEALVNMAVAAGLHIEMRVLEPA"
     gene            complement(2393..2731)
                     /locus_tag="VIR04_10645"
     CDS             complement(2393..2731)
                     /locus_tag="VIR04_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY9461246.1"
                     /translation="MPPSKPVEFLGSSLDDLRAFPPSAKREAGHQIDQVQNGWDPDDW
                     KPMNTVGQGVREIRVRESAGAFRVVYVAKFADAVYVIHCFQKKTEKTNKADIDLASKR
                     YWDLLQELSK"
     gene            2968..3087
                     /locus_tag="VIR04_10650"
                     /pseudo
     CDS             2968..3087
                     /locus_tag="VIR04_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(3183..3434)
                     /locus_tag="VIR04_10655"
     CDS             complement(3183..3434)
                     /locus_tag="VIR04_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461247.1"
                     /translation="MKILRLALRPVFVAVACAFISACANNPPRQTDLDNRTPSSPTTQ
                     GANGSRGSSGQRLTIQSGEGERLNLPWFVRDTQNWINGN"
     gene            3626..4084
                     /locus_tag="VIR04_10660"
     CDS             3626..4084
                     /locus_tag="VIR04_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461248.1"
                     /translation="MPRCLFAIAILIYSTVALAVPDDVGLGVKTFVNELKQQCASAYT
                     CNTNILPEIPIKAGDLAHCVMSNDAATKIEQRNNIYHNYYYLYPYKNTCPSMAHAFVK
                     VQGKWMKVAPIRKADERVITVLYSRSDGGAPNVIMFCKRSRLPTMDTRCP"
     gene            4095..4757
                     /locus_tag="VIR04_10665"
     CDS             4095..4757
                     /locus_tag="VIR04_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY9461249.1"
                     /translation="MTRVRVEGFTISLDGYGAGPSQDINNPLGIGGSELHQWLIPTRT
                     FQQALFGKDDGTTGVDDDFAARGFHNIGAWILGRNMFGPIRGDWPDTNWKGWWGDNPP
                     YHVPVFVLTHHGRPPIQMEGGTTFHFITGGMREALDRACEAASGMDVRIGGGPETIRQ
                     YLREGLIDELHIAIAPVLLGQGESLFEGVDLRALGYECVEFVSSQKATHVILRRQGHS
                     DA"
     gene            complement(4790..>5562)
                     /locus_tag="VIR04_10670"
     CDS             complement(4790..>5562)
                     /locus_tag="VIR04_10670"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY9461250.1"
                     /translation="VTLWIGSLAPLLYLLGRNDQASAQTLRRFSSLIVWVVVVIVAAG
                     VTLALLQFDHPQSLWQTIYGRVFLVKMVFLASLLAVAVYNRYRLTSGVLRGDARSIVT
                     MRRFIRIELALAVCILATVAAWRFTPPPRALAEAASAKPAFVHIHNTAAMVEVTIIPP
                     RAGEPASMQLALMSNNLTPLVPKEVTVYFSNVAAGIEPIRRDARRLDDGTWRIDRLDL
                     PSVHQWTVRIDALISDFDRVSLEGKIDLDYAGNHVGKP"
BASE COUNT         1245 a         1557 c         1536 g         1224 t
ORIGIN      
        1 ggcttgcctt cgcatcagtt ggcgcacgca ggcataaacc ggacgtttca aatccccaag
       61 ccctttcgca cacttaccgt gcgggaaaat gtccaggttg ccgccaccta cggccggcgc
      121 ggcaccagtt ccgacgaaat cgccgccgtc ctcgatctgc tggatctgac cgatcaggcc
      181 ggacgtaaag cggaagagct caatagcgtc catctgaaaa tgctcgatct ggcgcgcgca
      241 ctggcgacca agccggttct gctttgtctg gatgagctgg ccgcaggatt caaccccggc
      301 gaactgactc acgtcgtcga tcaggttcac tccatcgcac gttcgggagt tgcgatcatt
      361 gtcgtggaac acctgatggg attcatcgaa gatgtgaccg accgcgtgat tgttctgaat
      421 gcaggcaaag gcatattcga gggcctgttg tcggaggccg cggcaaatcg ccaggtcatc
      481 gacgtgtttc tgggtgatgc tcatgccgtc tgaattattc agcgcccgag acatccacgc
      541 gggttacggc cgcgttcagg tcttgtgggg cgcgggtctg tccgtgaacg aggcgcaaac
      601 tgttgtgctg ctgggcatga atggcgcggg taaaacaacc ctgatcaaaa ccatcatggg
      661 ccttatccat cagtcgaaag gcacaatcac cttcaatggc gccgacctgt cccacacccg
      721 cacagataaa cgggtgcgtg cggggatatc gtacatgtcg gaagttggct gctttcccgg
      781 cttgtcggtt gacgaaaaca tacgcatagg cggtcaattc atgttgccga gcgcgctgca
      841 tcagcgcatc ggcgagcttt atgaaatctt cccggccatc gccgcacgca aaaacgcact
      901 ggcaagcagc ctgtcgggcg gccagcgcaa aatggttggc attgccaaag cgctggctgg
      961 aaacccaaaa ctgctgatca tggatgagcc ttcagccggc ctgtcgccgt tgctggtgca
     1021 agaagtcatc gaagttttag cccgattccg aaaggaagga atatcgttcc tgatcgccga
     1081 gcaaaacgtc cggtttcttg aactggctga ccacgttgtg accatgtccg gtgggcgcat
     1141 gggctttagc ggaacggtcg aggccatgcg cgcggacgac gccttgcgca aggcctattt
     1201 cgacatcaaa ggcgagcaca gcaatgcggg ttaagcggcc gattcagtgc cgttgcaact
     1261 tgcaactggc ccggaaatca tgcataatct cgcttcttcg ctatgcatga aagactgcac
     1321 aaataggggg gtatagctca gctgggagag cgcttgcatg gcatgcaaga ggtcagcggt
     1381 tcgatcccgc ttacctccac catttactcg aagacgcaca tcgcaatgct acaatgtgcg
     1441 attgtttttg tcctgtcccc ttcgtctaga ggcctaagac accacccttt cacggtgggt
     1501 acaggggttc gaatccccta ggggacgcca ggctatattg ttcatgactc ttggtcctgg
     1561 gttgtaaacc gctgcggagc ggtagttcag ttggttagaa taccggcctg tcacgccggg
     1621 ggtcgcgggt tcgagtcccg tccgctccgc caaaaaattc agtaaaaaaa agcacttaca
     1681 ccatgtaagt gctttttttt cgtcccgtct tctattccaa agtcgggttt ttgttccaaa
     1741 gtttgggctt tctggctttc tgtgcgactg gaaagccgcg ccaggcctag ctttccaagc
     1801 aggccggcct gtacgaatac cggcacagga tgcgtgtatt cgcgccccac ttccctgctg
     1861 atttacgcac ccagatgggt gcgcattaca ctctgctcac catgctatgc gcaacacgta
     1921 ctccgggagc acgacgagcg acgttccgac gcccctgtac atgcctgcga aacgtagcgg
     1981 cctctttcgc aagggtttcc catcagagaa cgcaatatat atagtcgtca tggctcagcc
     2041 ctcaattacg tgtggtgtta atacatcccc ccagcgatgg cttcgtctgc cgatgaggat
     2101 gaacaccgga ttcgtcaggc aggttcaagc acacgcatct caatgtgcag cccagcggca
     2161 acggccatat ttaccagggc ctcaagacca aaaacattga tttttccacg catcaggtca
     2221 gagatgcggg gctgggtcac gcccaaaagt ttggcggctt cggtctggct catcttgacg
     2281 cgaattatgt gctgcttcag tgccgtcatg aggactgaac gaagcttcat gttctccgct
     2341 tcttcctggg tgtcttcgat ggcatcccag atgctggcaa atcgttggtt gctcatttgc
     2401 ttagctcctg taataggtcc caataacgtt tcgaggccag atctatatcc gccttgtttg
     2461 ttttctccgt ctttttctga aagcaatgga tcacatagac ggcatcggcg aacttggcaa
     2521 cgtagacgac cctgaaggcg ccagcgcttt cgcgaacgcg gatttctctg acaccttggc
     2581 ccactgtgtt cataggcttc cagtcatccg gatcccaacc gttctgaacc tgatcgatct
     2641 gatgaccagc ttcgcgcttg gcagaaggcg ggaaagcgcg aaggtcgtca agtgagctcc
     2701 cgaggaactc gacaggcttg gatggcggca tgcgtgattt atacaatatt ttgtatgttc
     2761 tggcaagacc aatgtcattc atttactggc gtatcggctg ctgtctcaat gatatgcagg
     2821 gaatccgccg catccgggtg aatttatatc tggcatctat ctcgcccgcc agcatgttga
     2881 tttacaaaat gtcgacgagc ttcgattcgc cgtcgcctag catccatgct ctcaaccgcc
     2941 atcacggggt ggtgtcaacg ttttggccag taccgtgtct ctgatctgac gcgtggaaaa
     3001 atcaacgttt tcggtcttga tgctctggta aatatggccc ctgctgccgg gctgcatatt
     3061 gagatgcgcg tgcttgagcc cgcgtgacga cccgggcgct attaacgccg gaatcatgga
     3121 cggacgaaag gcttactttt gggccagaag cggtcgctca cgaccagtga ctcgggtcgg
     3181 gactagttgc cgttaatcca gttctgcgta tctcggacga accagggaag attcaacctt
     3241 tccccctcgc cagactgtat ggtcaaacgc tgacccgacg agccacggga gccattcgca
     3301 ccctgtgtcg tgggagatga cggggttcga ttgtccaggt cagtttgacg gggcggatta
     3361 ttcgcacaag cggaaataaa tgcgcacgcg accgcgacaa aaacgggccg tagtgctaag
     3421 cgaaggatct tcataatgtt ctggttcccg ctggcggcac aggccaaagt gcctgacaat
     3481 caaggcctat attccgtacc ttactcgcaa tacgaggctt tcgtcagcat ctcgcgcccc
     3541 cgctaatcaa cccagcctat agaattcggc gcgacaacat gttaatgtcc gcccgtgcac
     3601 gcgccaccac ccaataggag cctccatgcc acgatgcctc ttcgccattg ccatcctgat
     3661 ttattcgaca gttgcgcttg cagtgcctga cgatgtcggc ctgggcgtca agactttcgt
     3721 gaatgagcta aagcagcaat gcgccagcgc ctatacgtgc aacacgaata tccttccgga
     3781 aattccaata aaggcgggtg atctggcgca ttgcgtgatg tcgaacgacg cggcgacaaa
     3841 aatcgaacag cgcaataaca tctaccataa ctattattat ctgtacccgt acaagaacac
     3901 gtgtccttcc atggcgcatg cattcgttaa agtccagggc aagtggatga aggtggcccc
     3961 cattcgcaag gccgatgaac gcgtcatcac ggttctttac agcagatcgg acggcggcgc
     4021 tccaaacgtc atcatgttct gcaagagaag caggcttccc acaatggata cacggtgccc
     4081 gtgaaatggc cgcgatgaca cgagttcgcg ttgaaggctt caccatctcg ctcgacggat
     4141 acggagcggg tccgagtcag gatatcaaca atccgcttgg cattggcggg tctgaactgc
     4201 atcagtggct gatcccgaca cgtacattcc agcaggcctt gttcggcaag gacgatggta
     4261 caaccggagt tgacgatgat ttcgccgctc gtggctttca caatattggc gcctggattc
     4321 ttgggaggaa catgttcgga cccattcgtg gggactggcc agacacaaac tggaaaggct
     4381 ggtggggaga taatccaccg tatcacgttc cggtctttgt cttgacccat cacggtcgtc
     4441 ctcccatcca gatggaaggc ggcacgacgt ttcacttcat aacaggcggc atgcgcgagg
     4501 cgcttgaccg tgcatgcgag gccgcttccg gaatggatgt gcggatcggc ggtgggccgg
     4561 aaacaatccg gcagtatctt cgtgaaggcc tcattgatga actgcacatt gctattgcgc
     4621 cggttctgct gggccaggga gaatcgctgt tcgaaggcgt tgacttgcgg gctctggggt
     4681 acgaatgcgt tgaattcgta tcatcgcaaa aagcgaccca tgtcatcctg cggcgccaag
     4741 ggcactcgga cgcctgacca ctcgctaaag gccgacgtct ctgatgacgc taaggtttcc
     4801 ccacgtgatt accggcgtaa tccagatcga tcttaccctc aagactaacc cggtcgaaat
     4861 ccgaaataag cgcgtcaatg cgtaccgtcc actgatgcac ggacggcaaa tccagccggt
     4921 caatgcgcca ggtgccgtcg tccaatcgtc tggcgtcgcg ccgaataggc tcgatgcccg
     4981 cggcgacatt cgaaaaatag accgtcacct ccttgggaac gagcggtgtg aggttattgc
     5041 tcataagcgc cagttgcatg ctggccggtt ctccggcacg gggcgggata atggtgacct
     5101 cgaccatggc ggcggtattg tgaatatgga caaaggcagg cttggctgac gcggcttcgg
     5161 ccaaggcgcg aggggggggc gtaaaacgcc aggcggccac cgtcgccaaa atgcaaacag
     5221 ccagtgcaag ctcgattcgg ataaagcgcc gcatggtcac gatggaacgc gcatcgccgc
     5281 gcaacacgcc gctggtcaag cggtagcggt tataaacagc caccgcgagc aaacttgcaa
     5341 ggaataccat ctttaccagg aagacccgtc cataaatggt ctgccacagc gactgcgggt
     5401 ggtcgaattg cagcaaagcc agagtgacgc ctgcagcaac aatcacgaca accacccaca
     5461 caatcaaact ggaaaaccgc cgcaatgtct gagctgacgc ctgatcgttg cgcccgagca
     5521 aataaagcaa cggtgccagt gagccaatcc agagcgtaac gg
//
LOCUS       DATCXU010000478         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_81924,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000478 DATCXU010000000
VERSION     DATCXU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81924"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VIR04_10675"
     CDS             <1..926
                     /locus_tag="VIR04_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY9461251.1"
                     /translation="ACSTCRGKVISGTYDAGRSPARILPPEDIAAGYTLFCQARPSSD
                     LVIEAHEIRMSSDIQIRKMPSRVLKLEQLAPDVMVLQLQLPAADPFRYYAGQYLEFIL
                     KDGRRRSYSIACMPSDDSRVELHIRHLPGGAFTDHVFGLDGNPMKVREILRTEGPFGS
                     FFLRDDSVKPIIFLASGTGFAPIKAIIERMDASHNPRPAVLYWGGRRPRDLYMHDKAL
                     QWARTIPGFSYVPVVSDAAPEDNWTGRTGFVHRAVLDDFPDLSGHQVYACGAPVMVDS
                     ARNDFVHLNGLPAEEFFADAFTTEADAVDAA"
     gene            1043..2446
                     /locus_tag="VIR04_10680"
     CDS             1043..2446
                     /locus_tag="VIR04_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9461252.1"
                     /translation="MKTIQFLRVPTIWRTLALSTATALLSTTLAQAATTNIEVWTSLN
                     QHNKQAFEKLVKAFNADQKEVSVKSKSFDSSDAIESALAGGAKSAAGPNLVQLDDDRD
                     PDTVAKKRSYVLPLYTLLAKHPISNAAWFLPADNPFLRDSRARLLAFPYMVEVPVMYY
                     NIDAFKKAGISPAAPARSWKSLQDQVVQLANNGSRQCPFTTDVPVSVNLENLAAVNNQ
                     LYATDDNGLAAKGKTTPRFSFDSLFVRHLSVMISWVRSEIMTRPDQESKATSRFANRE
                     CAVLLSSSSNMGWFNDARSLNFGVTGLPYYPDVAPTPGNPFVNGAALWAIKGHTADQD
                     KATAAFLSWLAQPKNASTWYQQTGFLPLTQQAFAATGDSYYKNLGAWKSLVAVYAKRP
                     SSTALGFRISNYPQIKAMFHKTLDRALSGQLPAVSALNSASAQASSMMGPSSGVASGS
                     SKAVHHSSKAKKTHKKQ"
     gene            2685..3650
                     /locus_tag="VIR04_10685"
     CDS             2685..3650
                     /locus_tag="VIR04_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HEY9461253.1"
                     /translation="MPRTLAITIVMLGLAGCASLPKWPFFVTGATAESRGEPAHYNFE
                     WRLSGDRAVAPAQVFDNGRQTWLQFAPGQPLPAIFERTGRGDRHLDFRRDGPYVVLPG
                     VWPNLILRGGRLKSFADRVADQSIKEPALSSSSGTPATVLAPEPMTTQAESSGVAPPV
                     QVAAIAGAGAVDSATGLNAPVAPEPSNLPVASQNASDAPIGDVDFDSAPMAVPLRDQQ
                     AAHDPSESYAVDPADKNLRVALSRWARTAGWIFEAEHWAVDADIPIVGSARFELTFKG
                     AVRELVASTELADRPLQPCFYSNKVLRVVPYAQSCNRTAGVPGSS"
     gene            3647..4495
                     /locus_tag="VIR04_10690"
     CDS             3647..4495
                     /locus_tag="VIR04_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461254.1"
                     /translation="MMRALRCFGIICLCGAASSCALGDKMREIGLLIGKTGTEIQDGH
                     EKFAQAVDSAQARRAAQEVDRPWLAGRSTPLARDVVLPLALRADVHTTMMFSGAALDL
                     PAVAQRITAVTAIPVYVRPDALLPIERFLPRLANAAQTGGLNQAPMTVALDGGPEPLA
                     RILDRISARLGVMWRYTSERIEFYRTETRVFNVRALTLNANAEASLGLSGGSGSEGFA
                     STSKTVLSGGSAELLAVVRARLEPFLSRAGLLVAEPGAGSLIVVTDTPDVLNALLCIW
                     SKKTGR"
BASE COUNT          928 a         1278 c         1325 g         1072 t
ORIGIN      
        1 gcgcttgctc gacctgccgc ggcaaggtga tatccggaac ctacgatgcc ggccgcagtc
       61 ccgcgcgcat attgccgccc gaggatatcg ccgcggggta tacgctgttt tgccaggcca
      121 ggccaagctc cgacctggtg attgaagcgc acgaaatccg gatgtccagc gatattcaaa
      181 ttcggaaaat gccctcgcgt gttctgaagc tggaacagct tgcccctgac gtgatggtcc
      241 ttcagttaca actgccggcc gccgacccgt ttcggtatta tgcgggccag taccttgaat
      301 tcattctgaa agatggccgg cgcagaagtt attcgattgc ctgcatgccg tccgacgata
      361 gccgggtcga attgcacatt cggcacctgc cgggcggcgc tttcactgac cacgtgttcg
      421 ggttggacgg caacccgatg aaagtgcgcg aaattctacg caccgaaggg ccttttggct
      481 ctttcttcct gcgcgacgac agcgtgaagc ccatcatctt cctggccagc gggaccggtt
      541 ttgcccctat caaggctatc attgaacgca tggacgcaag ccataacccg cgtccggccg
      601 tcttgtattg gggtgggagg cgcccgcgcg acctttatat gcatgacaaa gccctgcagt
      661 gggctcggac tattcctggg ttttcatacg tgccggttgt ttccgatgcg gcgcccgaag
      721 ataactggac ggggcgcact ggttttgtgc accgcgccgt gctggatgat tttccggatt
      781 tgtcaggtca tcaggtgtat gcgtgtggcg cacccgtcat ggtcgacagc gcgcgcaatg
      841 attttgttca cttgaatggc ttgccggccg aagaattttt cgcggatgcg tttaccaccg
      901 aagcggatgc ggtcgacgcg gcgtgaatac aacccgttgc aaaaataagg cgcaacaggc
      961 gtggatttcg tttccaagcg ttccggattt tgatactgtc cacaacaacc aatcaaagtt
     1021 tcctttttag gccaaccagt ttatgaaaac aattcagttt ttacgcgtac ccactatttg
     1081 gcgcacgctg gcactaagca ctgcgacagc gctgttaagc acaacgctag cccaggccgc
     1141 tacaaccaat atcgaggtgt ggacttcgct caatcagcac aataaacagg catttgaaaa
     1201 gctggtcaaa gcgtttaatg ccgaccaaaa agaagtcagc gtcaaatcaa agtcgttcga
     1261 ttcctccgat gcaatcgagt cggctctggc aggcggcgca aaaagtgcgg ccggcccgaa
     1321 tctggttcag cttgacgatg atcgcgatcc cgacacagtt gccaaaaagc gcagctacgt
     1381 gttgccctta tacacattat tggcgaagca tcccatcagc aacgccgcct ggtttttgcc
     1441 cgccgacaat cctttcctgc gcgatagcag ggcgcgcttg ctggcctttc cctatatggt
     1501 tgaggttccg gtaatgtatt acaacatcga cgcgtttaaa aaggctggaa tcagccccgc
     1561 tgcgccagca cgctcatgga aaagcttgca ggatcaggtc gttcagttgg ccaacaacgg
     1621 gtcgcggcaa tgcccgttca cgaccgacgt gcctgtgtcg gtgaatctgg aaaacctggc
     1681 cgccgtcaat aatcagcttt acgccacgga tgacaatggc ctggccgcca agggtaaaac
     1741 cacgccgcgc ttttcgtttg attccttgtt cgtaaggcat ttgtcggtaa tgatcagttg
     1801 ggtgcgttcc gaaataatga cccggcccga tcaggaatca aaagcgacct cgcgttttgc
     1861 caatcgcgaa tgcgcagtgt tgttgtccag ttcaagcaat atgggctggt tcaacgatgc
     1921 gcgttcactg aattttggcg ttaccggttt gccgtattac cccgatgtgg ccccaacgcc
     1981 gggaaacccg ttcgtcaatg gcgctgcgtt atgggcaata aagggtcaca cggcggacca
     2041 ggacaaagca accgccgcct ttctaagctg gctggctcag cccaagaatg cctccacgtg
     2101 gtatcagcaa accgggttct tgccgttgac ccaacaggct tttgccgcga ccggcgacag
     2161 ctactataaa aacctgggtg cgtggaaaag cctggttgcc gtttatgcga aacgtccatc
     2221 gtcaacggcg ctgggctttc gaattagcaa ttatccacag ataaaagcca tgttccataa
     2281 aaccctggat cgtgcgctaa gcggacaact gcccgcagta agcgccttga attcggcttc
     2341 ggcccaagcg tcgagcatga tgggcccttc gtcgggtgtt gcgtcggggt cgtcaaaggc
     2401 ggtgcatcat tcttcaaaag cgaaaaagac gcacaagaag cagtagattc ttttacggat
     2461 cttgaacccc gcaggtcaaa tttggcctgc ggggtttttt tatttcttgc gcaggcaata
     2521 tttttaactg attgattaaa tggaaatatt tttgtttccc aacggcctct ggttacgata
     2581 aaaaattcca ttcgtataag tcgatctggc ggcgacgcga ttcgatgcgc aagataagag
     2641 ccgtgtgcgg gacatggcgt ctttttctta ttttggtgcg gattatgccg agaacactgg
     2701 cgattacgat tgttatgctt ggcctggccg gttgcgcaag cttgccaaag tggcccttct
     2761 ttgtaacggg cgctacggcc gagtcgcgcg gcgaacccgc ccactacaat tttgaatggc
     2821 gcttgtcggg tgaccgcgcc gttgctccgg cgcaggtttt tgataatggg cgccagacat
     2881 ggctgcagtt tgctcccgga cagccgttgc cggccatctt tgaacgcacc ggccgtggtg
     2941 atcgccacct ggattttcgc agggatgggc cttacgtggt tttgccgggc gtatggccga
     3001 atctgatatt gcgcggcggg cgtttaaaga gctttgccga tcgcgttgct gaccagtcga
     3061 ttaaagaacc ggcgctgtcg tcgtcttcgg gcacgcctgc gactgtcctc gcgcccgaac
     3121 cgatgacgac tcaggcagaa agcagtggcg ttgcgccgcc tgttcaagtg gcagctatcg
     3181 ctggcgccgg cgctgttgat tcagcaactg gtctgaatgc gcctgtcgcg ccggaaccgt
     3241 caaatctgcc cgttgcttcg caaaacgcct ccgacgcgcc gataggcgac gttgattttg
     3301 actctgcgcc gatggcggtg ccattgcgtg accagcaagc cgcgcacgat ccgagcgaga
     3361 gctatgccgt cgacccggcg gataaaaatc tgcgagttgc attgtcacga tgggcgcgaa
     3421 cggctggttg gatattcgag gcggaacact gggcggttga tgccgatatc cccatagtag
     3481 gttcggctcg tttcgaattg acattcaaag gagcggtgcg cgaacttgtt gcgtcaactg
     3541 aactggccga tcgcccattg cagccctgtt tttactccaa taaggtattg cgggttgtgc
     3601 cttatgctca atcgtgtaat cggactgccg gcgtgccggg gtcgtcatga tgcgcgcgtt
     3661 gcggtgtttc ggcattattt gcctgtgcgg cgccgcttcg agctgcgctc ttggcgataa
     3721 aatgcgcgag atcggcctgt taatcggaaa gacaggaacc gagatacaag acggccacga
     3781 aaagtttgcc caagccgtcg acagtgcgca ggcgcgccgg gccgcgcaag aggtggatcg
     3841 cccctggctt gccggccgct cgacaccgct tgcccgcgat gtggtcttgc cactggcgtt
     3901 gcgcgcggac gtccacacga ccatgatgtt ttctggtgcg gcgctggatc tgccggctgt
     3961 cgcgcagcgc attacagccg tcacagccat tcccgtttat gtgcggcccg acgccttgct
     4021 gcctatcgag cggtttctcc ctcgccttgc taacgcggcg caaactggcg ggctcaacca
     4081 ggcgcccatg acggtggctc tggacggcgg ccccgagcct ctggcgagga ttcttgatcg
     4141 tatcagcgca cggttgggcg ttatgtggcg ctatacgagc gaacgcatcg agttttatcg
     4201 caccgaaaca cgggtattca atgtccgcgc gctcacgcta aatgccaacg ctgaagcatc
     4261 tctgggttta agtggcggct ctggctcgga aggctttgcc agcacctcaa aaaccgtgtt
     4321 gtcgggcggc tccgctgaac tgttggccgt cgtgcgcgca cgtcttgaac cctttctgtc
     4381 ccgagccgga ctgctggttg ccgaaccggg cgccggttcg ttgattgtcg taaccgacac
     4441 ccccgatgtt ttgaacgcat tgctgtgtat ctggagcaag aaaacaggac gctgacacgc
     4501 cgggtgcgtt tgatattcga agaggttacg ctcgctactc acgattccgc cgaggcgggt
     4561 attgattgga atttggtgtt ttccggcgcc cggcttgctg cgg
//
LOCUS       DATCXU010000479         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_291971,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000479 DATCXU010000000
VERSION     DATCXU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291971"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VIR04_10695"
     CDS             <1..530
                     /locus_tag="VIR04_10695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY9461255.1"
                     /translation="GKIRSAFFESALGASQIEFIGKDIVFCEYNGRPALRLALPASLK
                     RTQRRESFRIAVPAMNPARCTLPAPAQVEGKVTLPVQNISAGGLLLLDDEQLLDNTVG
                     AQFDDCQIDLPEVGWIVVKLRVVHNENIPLKSGRTGHAVGFAFVDISDATLTGIWHYA
                     SRLERSIIARESDSD"
     gene            complement(555..974)
                     /locus_tag="VIR04_10700"
     CDS             complement(555..974)
                     /locus_tag="VIR04_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461256.1"
                     /translation="MITRTPPLLRTLFLSLATLFLASPAQGGNTFNARIIITSQCSAQ
                     QALFGPQAQHSATITCLPGSTPFQTQAISLGSNTVNMDQSESKDSSELKTGADSGQQS
                     QSATSGAITSSNSTKTILSPPLPTSSAFASKVVYVVF"
     gene            complement(1029..2042)
                     /locus_tag="VIR04_10705"
     CDS             complement(1029..2042)
                     /locus_tag="VIR04_10705"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEY9461257.1"
                     /translation="MHTLILKARRPSIRYVLIALMLVYAMLYCGVSQAAPTPSLNCSA
                     TTADNLNFGNVDALAGIASGVSGIISYSCTNTQNNSNGSVLLCFNIGTGTNSTSSYDP
                     RQINATPTGALQYRIYRDGPGGQIWGNTNATTAFVPTFNMAKGDIASGTIIMYGSIAA
                     NQNSITPGTYQSDFTAKLMWAADSTPNLTLNCSSATNSGGTFTFSVIANVIKRCAITA
                     TDLNFGAVSNIGTASVNGSTQIGISCSNGLPFQVGLDNGVNSPGNTRNMKKTNGAELM
                     RYELYRDASRSERWGNTKDSDTVSQTGSGSSVAINVYGQAYPAPGTTAGDYADTVTIT
                     LYY"
     gene            complement(2030..>3058)
                     /locus_tag="VIR04_10710"
     CDS             complement(2030..>3058)
                     /locus_tag="VIR04_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEY9461258.1"
                     /translation="GYGSIPAIRSDSAYIGLDTGKFGSIGFNYTNLQQADQAAFRYGG
                     VSWSRSFNSGISLSISGNQNLNDRGDRSIYLNLSFNWDKGVSAYSSAVQSKGANTYSV
                     GVNRSAPIEGDWSWSVQGQQADQQGASASAQVSTRMRYLDFNAGANSAGASQNAYAGA
                     SGSIVAMGGGVFASRRIYDGFAIVSTDGVPDVPIKNQNRPVGNTDSRGLLLVPGLQSY
                     QDNKISIDPTDLPIDMHIDRVNLNVVPRLASGLNVKFKMERVLAASLILHRPDGQNVA
                     MGGQVFLNGSQQTAGWVGYDGRVYVEGLKADNHLLVQDDGENCAVRFPYHAKADTLPE
                     IGPLVCTP"
BASE COUNT          626 a          780 c          903 g          749 t
ORIGIN      
        1 tgggcaaaat ccgcagtgcg ttttttgaat ccgcgctggg cgcaagccag attgagttca
       61 ttgggaagga tatcgtcttt tgcgaatata acggtcggcc ggctttgcgt ctggctttac
      121 ctgcgtcgct taaacgcaca caacggcggg aatcgtttcg catcgcggtt ccggccatga
      181 atcccgctcg ttgcacgttg ccggcgcccg cgcaagtgga aggcaaggta acgttgcctg
      241 tgcaaaatat tagtgccgga ggcctattgc tgctggacga cgagcagttg cttgacaaca
      301 ctgtcggcgc ccagttcgat gattgccaga ttgatttacc tgaggttggc tggatcgtgg
      361 ttaagctgcg cgtcgtccat aacgaaaata ttcccttgaa atcgggccgg accgggcatg
      421 ctgtgggatt tgcttttgtc gatatctccg atgccactct gacgggaata tggcattacg
      481 catcgcgcct ggagcgttcg atcatcgcgc gtgaaagtga ttcagattaa cgacaggcat
      541 gtcgtgcaat tgggctaaaa aacgacatac accacttttg atgcgaacgc agaagaagtg
      601 ggcaaaggcg gcgacaatat agtcttggtc gagttgctgg aggtgattgc gcctgaggtc
      661 gccgattgac tctgctgtcc ggagtcggcg cccgttttta attcacttga atccttggat
      721 tcgctctggt ccatgttgac ggtgttgctg cccagactga ttgcctgggt ctggaagggt
      781 gtgctgccgg gcaagcatgt gatcgtggcg ctgtgctgag cctgcggtcc gaacaaggct
      841 tgctgcgcgc tgcattgcga tgtaatgata atacgggcat tgaacgtatt gccgccttgg
      901 gccgggctgg ccagaaacag ggtggcgagc gacaaaaaca aggtcctcag caacggcggc
      961 gttcgtgtga tcatgctttt atactaggtc caagggcgtg tgtcgagact atagcatgtg
     1021 ggcttcagtc agtaataaag cgtaatagtt acggtgtcgg cataatcgcc agcagtggtt
     1081 ccgggggcgg ggtaagcttg cccgtagaca ttaatggcga ccgacgaacc tgatccggtc
     1141 tggcttacgg tatccgagtc tttcgtgtta ccccaccttt cgctgcggct cgcgtcccta
     1201 tataactcgt agcgcataag ttctgctcca ttggtctttt tcatattgcg agtattgccc
     1261 gggctattga cgccattatc cagtccgact tgaaacggta agccgttgct gcagcttatg
     1321 ccgatttggg tagaaccatt tacagaggcg gtgccgatat tggaaactgc tccgaaattc
     1381 aggtcagtgg cagtaatggc gcaccgtttg atcacgtttg caataaccga gaatgtaaag
     1441 gttcctccac tgttggtggc gctgctgcaa tttagcgtca ggttaggcgt cgaatctgct
     1501 gcccacataa gcttggcagt gaaatcagat tggtaggttc ccggtgtgat gctgttctgg
     1561 tttgcagcaa tggatccgta catgatgatg gtgccagaag caatgtcgcc cttggccatg
     1621 ttgaaggtgg gcacgaaggc ggttgttgca ttggtgttgc cccagatttg tccgcctgga
     1681 ccgtctctgt aaattcgata ttgcagcgct ccggtcggcg ttgcatttat ttgccgcggg
     1741 tcataagagc ttgtgctgtt tgtccccgtg cctatgttga aacacaagag gacagacccg
     1801 ttgctattat tctgtgtatt cgtgcaggag taactgatta tgccggatac gccggaagct
     1861 atccccgcca gtgcgtcgac gtttccaaag ttcaggttgt cagcagtggt ggccgagcaa
     1921 ttcagactgg gcgtaggggc agcctgcgac acaccgcagt aaagcatggc atagaccagc
     1981 atcaaggcaa tgaggacata gcgaatgctt gggcgtcgcg ctttcagaat cagggtgtgc
     2041 ataccaaggg tcctatttcc ggcagggtgt cagcctttgc atgataggga aaccgcacgg
     2101 cgcaattttc accgtcgtct tgcacgagca aatggttgtc ggccttcagg ccttcgacgt
     2161 aaacacggcc gtcgtaaccc acccaacccg cggtttgctg gctaccattc aagaacactt
     2221 gaccgcccat ggccacgttt tggccgtcag ggcgatgtag tatcagggag gccgccagca
     2281 cacgttccat tttgaatttg acgttcaggc ccgatgccag gcgcggcacc acgttcaaat
     2341 tcacacgatc gatatgcatg tctatcggta aatccgtggg atcgatagag attttattgt
     2401 cttgatagga ctgcaagccg ggcaccagta aaaggccgcg gctgtcggtg ttgcccacag
     2461 ggcggttctg gttcttgatc ggcacgtcag gcactccgtc ggtcgataca atggcgaacc
     2521 catcatagat ccggcgcgag gcaaacacgc cgccacccat agccacaatc gagcccgacg
     2581 cgccggcgta agcgttctgg ctggccccag cgctgttggc cccggcgtta aagtccaggt
     2641 agcgcatgcg cgtgcttacc tgcgcgctgg cgctggcgcc ctgctggtcg gcttgctggc
     2701 cttgcacgct ccagctccag tcgccttcga tgggggctga ccgatttacg ccgaccgaat
     2761 atgtgttggc gcctttcgac tggactgcgg agctataggc cgagactcct ttgtcccagt
     2821 taaatgacaa gttcagataa atagagcggt caccacggtc gttcagattc tggtttccgc
     2881 taatgcttag cgaaatgccg gaattgaaac tgcgtgacca cgataccccg ccgtagcgga
     2941 aagcggcctg gtcggcttgt tgaaggttgg tgtagttgaa tccgatgctt ccgaatttgc
     3001 cggtgtccag gccgatgtag gcgctatcgc tgcgaatcgc ggggatgctg ccgtagcc
//
LOCUS       DATCXU010000480         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_133283,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000480 DATCXU010000000
VERSION     DATCXU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133283"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1375
                     /locus_tag="VIR04_10715"
     CDS             197..1375
                     /locus_tag="VIR04_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461259.1"
                     /translation="MKKSLFIAAIAVAVSTSLAPVISSAETGVTKDTIRIGMFGPLTG
                     PTAVGSLPLLGASAIYKNVNDAGGINGRKIELFIEDDACEPNKTISATKKLISQDQVF
                     MIHGGWCSGTVMAIKPELARNPTLPFMVLGAASAAISTPMTPNIFHPIATTAAVTKAM
                     LDFAESKPGAKRIAIISHSDDWGKSHMTPLLARMKAQGIKPVESVWLERGSADATSQL
                     LKIRAAKPDVVLAILYPPELTLYLRGANKYGVTAPTVTTQGVSIEDMVKRVGNSAATK
                     HLFVFYPLSETLDAPDFKKWIDIYRKYNPKDPVETLSFMGMTGALAIVEAIKRAGPDL
                     TREKVIAELDKLRDFDPGIQSGALTFTPEVHAGITTGKMIYWPTDRPEIVSSYPVAQK
                     "
     gene            1452..2321
                     /locus_tag="VIR04_10720"
     CDS             1452..2321
                     /locus_tag="VIR04_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461260.1"
                     /translation="MFAQLIASGIVNGGVYAVVALGMTILYRSTTIVNFCHGEFFMLG
                     AFAVLVSQQMFGWSYAGSIAFALILLFVVGSGVERALMRPLQNAPHISLAMMTVALSY
                     LFRGVSRFFYGREVEPLEPLITGEPIEIGSVILMPQDILIACFSLVVVVGFFYFFQKT
                     ETGKVIQAASQTPRGASLVGINVPLLRNLMWGVGAVMGAVAGILIAPNTLVYPDMGGH
                     MLIQAFAAMTLGGFGSLPGAVLGALALGVLENLIGGYISSSLVEITAYLAIIVVLIVR
                     PQGFLGEWRQGRG"
     gene            2314..3309
                     /locus_tag="VIR04_10725"
     CDS             2314..3309
                     /locus_tag="VIR04_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461261.1"
                     /translation="MANPTNSKNLARGQGRSKRNAIFSLLVLLCLLPLAVNDYQQFVA
                     NTMLVYCLVAIALNIVLGYLGQLAFANAAFFGIGAYATGLSMVHLGASFPVAMVLGAA
                     CGGLAGVIVGLPALRVRGYYLAIITLAFGELMRWVYVHQDKFTFGSGGFTVPPITFFG
                     PSLSESGKYYLFLAIVVISVGATSLLLRSRYGRAFVAVRNNELAAASLGINVNRTKVI
                     AFAWSGLIAGLAGSLYAALNGRVSPDTFGIAQMLMHFAIVMIGGLGSLIGSIIGAVLL
                     TGLPELLRNFPGLEEIVFSLLLIIVLFLMPKGISGYLMGHVSLLRERLYREKDNA"
BASE COUNT          666 a          889 c         1018 g          825 t
ORIGIN      
        1 acggggaaac gattcccggc ttgtatgccg ccggagaaat gaccggcctg tattacggta
       61 cttataccgg agccacctcg gtgttgcgcg gcgctgtatt tggccggata gcgggatcgg
      121 aagccgtaca tcgggattaa ccatcacact tgcgagcagg tccggtaacc ataccggcca
      181 ataacaggag acagcaatga aaaagtcatt gtttattgca gcaatcgcgg ttgcggtatc
      241 aacatctttg gcgcccgtta tatccagcgc cgagaccggc gtgaccaaag acaccatacg
      301 catcggcatg ttcggtcctt tgaccggccc caccgccgtg ggttcgctgc cgctgctggg
      361 cgccagcgcg atttacaaaa acgtgaacga tgcgggcggc atcaatggcc gcaaaatcga
      421 actctttatt gaagacgacg cgtgcgagcc gaacaaaacc atttcagcca ctaaaaagct
      481 gatttcgcag gatcaggtgt tcatgattca cggcgggtgg tgcagcggca cagtcatggc
      541 catcaagccc gagctggctc ggaacccaac attgccgttc atggtgctgg gcgcggccag
      601 cgccgcgatt tcgacgccga tgacgccgaa tatttttcat cctatcgcaa ccacggccgc
      661 cgtcaccaaa gccatgctgg acttcgccga gtccaagccg ggcgccaagc gcatagccat
      721 catcagccat tccgatgatt ggggcaaatc gcatatgacg ccgttgctgg cccgcatgaa
      781 agcccagggc attaagcctg tggagtcggt ctggcttgaa cgcggcagcg ccgacgcgac
      841 ctcgcagttg ctcaaaatac gggccgcaaa gcccgatgtg gtgctggcca ttttatatcc
      901 gccagaactg acgctttatc tgcgtggcgc gaacaagtat ggcgtaacgg cgcccacggt
      961 gacgactcag ggcgtgtcga tcgaagacat ggtcaagcga gtgggcaact ctgcagcgac
     1021 aaagcatttg tttgtgtttt acccattgtc cgaaactttg gatgcccccg actttaaaaa
     1081 atggatcgac atttatcgga aatacaaccc gaaagatcct gtggaaacgc tcagctttat
     1141 ggggatgacc ggggcgctgg ccatcgttga agcaatcaag cgcgcagggc ccgatctgac
     1201 tcgtgaaaag gttattgccg aactggacaa gttgcgcgat tttgatccag gcatacagtc
     1261 cggcgcattg acgttcaccc ccgaggtgca tgcgggaatc acgaccggaa aaatgattta
     1321 ctggccgacg gatcgtccgg agattgtcag ttcttatccc gttgcgcaga aataaatgct
     1381 gcctgcgagg taactcgttg actgagtgcc gctaaggcgc ccagtcaact tcgattgaaa
     1441 aagggatgtc catgttcgcc cagcttattg cgagcggcat cgtcaacggc ggcgtatatg
     1501 cggtggtcgc ccttgggatg accatactgt atcgctcgac gacgatcgta aatttttgcc
     1561 atggcgagtt tttcatgctt ggcgcattcg ctgtgctggt gtcgcagcag atgtttggct
     1621 ggtcgtacgc gggttcgatc gctttcgcgc tgatattgct atttgttgtc ggcagcggcg
     1681 tggaaagagc tctgatgcgc ccgctgcaga atgcgccgca tataagtctg gcgatgatga
     1741 ctgtggcgtt gtcctatctg ttccgtggcg tctctcgctt tttctacggg cgtgaagttg
     1801 agccgctaga gccattaatt accggagagc cgattgaaat cggcagcgtg atcctgatgc
     1861 cgcaagacat tttgattgcc tgtttctcgc tggttgtcgt tgtcggtttc ttctactttt
     1921 tccagaagac cgagaccggt aaagtaattc aagccgcctc gcagacgccg cgcggggcat
     1981 cgctggtcgg catcaacgtg ccgctgctgc gcaatttgat gtggggcgtg ggtgcagtca
     2041 tgggcgcggt ggcgggaatt ttaattgcgc ccaatacgct ggtttacccc gatatgggcg
     2101 gccacatgct gattcaggcc tttgccgcca tgacgcttgg gggtttcggt tcgttgccag
     2161 gggctgtgtt gggggcgctg gcgctgggtg tgcttgaaaa tttgatcgga ggttatattt
     2221 ccagctcgct ggttgaaatc accgcgtatc tggcaattat cgtagtgttg atcgtgcggc
     2281 cgcaaggctt tctgggcgaa tggaggcaag gtcgtggcta acccgacgaa ttcaaaaaac
     2341 ctggcgcggg ggcaggggcg ttccaagcgc aatgcgatct tctcgctgct agtgctgttg
     2401 tgcctgttgc cgctggccgt caacgactac caacagtttg tcgccaatac gatgctggtg
     2461 tattgcctgg tcgcgattgc gctgaacatt gtgcttgggt atctgggcca attggcgttc
     2521 gcaaatgccg cattctttgg catcggcgcc tatgccacag ggctgtccat ggtgcatttg
     2581 ggcgcatcgt ttccggtagc catggtgctg ggcgccgcgt gcggcggcct ggctggggta
     2641 attgtgggct tgcccgcatt acgtgtgcgc gggtactacc tggccatcat cacgttggcg
     2701 tttggcgagc tgatgcgctg ggtttatgtg caccaggata aattcacgtt cggttcgggc
     2761 gggttcaccg tcccgcccat tacctttttt ggcccgtcgc tgtcagagtc gggaaaatac
     2821 tacttgtttc tggctatcgt ggtcatcagc gtcggtgcga cttctttatt gcttcgatcg
     2881 cgctacggcc gcgcatttgt cgctgtcagg aataacgaat tggccgccgc gtcgctgggc
     2941 atcaatgtta atcgcaccaa agtaatcgcc ttcgcgtgga gcggcctgat cgccggcctg
     3001 gcgggctctt tgtatgcggc gctaaacggc cgggtttcgc ccgacacctt cggcatcgcg
     3061 caaatgctga tgcacttcgc cattgtgatg attggcggcc tgggcagcct gatcgggtcg
     3121 atcatcggcg cggttttgtt aaccggcttg ccggagctat tgcgtaattt tccgggcctg
     3181 gaagaaatcg tcttcagcct tttgctgatt attgtgctgt tcctgatgcc caaaggcatt
     3241 agcggctacc ttatgggaca cgtgtcgttg ttgcgtgagc gcttgtaccg ggagaaggac
     3301 aatgcttgaa atccaaaacc tgacagtcaa atttgccggc ctgacggcgg tggacgactt
     3361 gtcgttcagc gtgaaggcgg gcgaagtatt tactttga
//
LOCUS       DATCXU010000481         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_604513,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000481 DATCXU010000000
VERSION     DATCXU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604513"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2136
                     /locus_tag="VIR04_10730"
     CDS             <1..2136
                     /locus_tag="VIR04_10730"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEY9461262.1"
                     /translation="EPLLVDLLDDAVQKNRRWPWRAALVFQISQTCATYFDRHEADWR
                     ASGSPSLYGFWRESMVGDHGLSSRLGLPGFMKQLRKLPLQAEDAMRWAIAELSLDETV
                     WEDYFEALLLSINGWASWCSYIGWQKIPAGQDDPRLRELLACRVAWEALLACAVPPAA
                     RRAAIAALVANWRDLDRRVAEANAALVVDELWQQALEASFQRGFGRKLCTPVSSDAMQ
                     NPEVQAVFCIDTRSELMRRSLEAVWAGVETRAFAGFFGLPIHYNPLGTDIHRPQLPGL
                     AYPSIAVEDVMTAHGQAAPVGGTKLAQIGSIRRKRLARLDQWLGAVRWPNTTFSFVEA
                     FGIGYLGKIAHWIKPPATPRASKDRIGLPASAQEQCRPMVTGLGVEKQVELAADVLHT
                     MGLDDRLAPLVILCGHAGHTTNNAHASTLDCGACYGRPGEANARALAHLLNDPRVREG
                     LRLRGLDIPANTVFMGALHNTTTDEVTGFDLDLLPPAAQARWKKMEQVFEQAGDRIRR
                     ERAPTLWMDPNLGPKALLKSFRRRANDGSQTRPEWGLTRNAAFVIGPRDRSRGRTLSR
                     TYLHDYDTQFDRDGSVLEKLMMGPMLVTHWLSWQYHASTCDPLHYGAGNKVLHNVVDG
                     HVGVFEGNGGDLRIGLPRQSLHNGDGWYHQPVRLTVVIDAPAVAIENVIARNAVLGRL
                     CDNGWILLWRYEGNALRRYELGHWLPLEV"
BASE COUNT          412 a          599 c          711 g          471 t
ORIGIN      
        1 gagccgttgt tggtggattt gcttgacgat gcggtgcaga agaacaggcg ctggccgtgg
       61 cgggcagccc tcgtgtttca gatcagccag acttgtgcga cgtacttcga ccgccatgag
      121 gcagactggc gtgcttcggg tagcccaagc ctgtatggtt tctggcgcga aagcatggtt
      181 ggcgaccatg gcttgagctc gcgactgggc ttgcccgggt tcatgaaaca gttgcgcaaa
      241 ctgccattgc aggcagagga tgcgatgcgt tgggcgattg ccgagctttc actcgatgag
      301 acggtttggg aggattactt cgaggcgctg ctgctatcga tcaatggctg ggcttcgtgg
      361 tgctcatata tcggttggca gaagattcct gctggacagg atgatccgcg cctgcgtgaa
      421 ttgcttgctt gccgggtcgc gtgggaagcc ttgcttgcct gcgccgtgcc gcccgctgca
      481 cggcgcgccg cgattgccgc acttgttgca aactggcgtg atttggaccg gcgtgtcgcg
      541 gaagccaatg cggccctggt ggtggacgaa ctctggcagc aggcgctcga agcaagcttc
      601 cagcgagggt tcggacgcaa gctgtgcaca ccagtgtctt cagacgcaat gcagaacccg
      661 gaggtccagg ctgttttctg cattgatacg cgcagcgagc tgatgcggcg ttcactggaa
      721 gccgtatggg ctggcgttga gacccgtgcg ttcgcgggat tcttcggctt gcccattcat
      781 tacaatccgc tgggcactga cattcatcgg ccgcaattgc ccgggctcgc ctatcctagt
      841 atcgcggtgg aggatgtgat gacggcgcac gggcaagccg caccggtggg cggtacgaaa
      901 ttggcgcaaa tcgggtcgat tcgccggaaa cggttggcgc ggctcgacca gtggctgggt
      961 gcggtgcgtt ggccaaacac gactttttct ttcgtcgaag cgtttggtat tggctatctc
     1021 ggcaagattg cgcactggat caagccgccg gcaactcccc gggccagtaa ggatcgcatt
     1081 ggattgcctg catccgcgca ggaacagtgc cggccgatgg ttacggggct gggtgtggaa
     1141 aagcaggtgg aactggcggc tgacgtgttg cacacaatgg gtcttgatga ccgtcttgcg
     1201 ccactggtta tattatgcgg ccacgccgga cacacaacca ataatgcgca cgcgtcgact
     1261 ctagattgtg gggcatgcta tggccggccg ggcgaagcca acgcgcgtgc gctggcgcat
     1321 ttgctgaacg accctcgggt ccgcgaaggc ctgcgcctgc gtggactgga catccccgct
     1381 aatacagtct tcatgggagc gttgcacaat acgaccaccg acgaagtgac cggcttcgac
     1441 cttgatcttt tgccgccagc ggcccaagcg cgctggaaga agatggagca agttttcgag
     1501 caggcgggtg accgtattcg tcgcgagcgt gcaccgacgc tgtggatgga tccgaacctt
     1561 ggccctaaag cattgttgaa atcttttcgg cgcagggcca atgatggttc tcagactcgg
     1621 ccagagtggg gtctgactcg caacgccgcg ttcgtcatcg gcccgcgtga tcgcagccgt
     1681 ggcagaacac tcagtcgcac ttaccttcat gactacgata cgcagtttga tagagacggc
     1741 agcgtgctcg agaaacttat gatggggccg atgctagtca cccactggct ttcctggcag
     1801 tatcacgcct caacctgcga cccgctgcac tatggcgcag gcaacaaagt actgcacaat
     1861 gttgtggatg gccacgtcgg cgtgttcgaa ggtaatggcg gcgatcttcg cattggcctg
     1921 cccaggcagt ccttgcataa tggtgatggc tggtatcacc agccggtccg cttgaccgtc
     1981 gtgatcgatg cgcccgcggt cgcgattgaa aacgtcatcg cgcgcaacgc cgtgctgggc
     2041 agactgtgcg ataacggctg gatactgttg tggcgttacg agggaaatgc gctgcggcgc
     2101 tacgagcttg gccattggct gcctcttgaa gtctgacgaa ccgcattaag aagtacggcg
     2161 ctccatactc tcgcgcgagt ggaacagact aag
//
LOCUS       DATCXU010000482         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_761947,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000482 DATCXU010000000
VERSION     DATCXU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761947"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIR04_10735"
     CDS             <1..535
                     /locus_tag="VIR04_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY9461263.1"
                     /translation="YAWGKASDDLNTIRGVPLPKDLNQMTGPQLYDANCASCHQARGQ
                     GSPSGGLPALFHNTALGRTNTNNLVMVLLDGIERRPDAPEVLMPGFRSELSDTQIATL
                     GTYLTQHYGNPGAPVTVEQVKTLRAAGQPSYLIWFARAGLVIAGLIIIFLFLLTQRRF
                     TRRSTDTRHPTQLNRRT"
     gene            457..906
                     /locus_tag="VIR04_10740"
     CDS             457..906
                     /locus_tag="VIR04_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY9461264.1"
                     /translation="MLVDSTQVHEKVHRYTPPNAVKQENIMDRFLTIAARVLMAQLFI
                     LSGWMKITGYSGTVGYFGSMGLPGILVPLVIIIELGGGLLLLFGYKTKWVAIILALFT
                     LGSALIAHLHLSDPLQFINFTKNLAITGGLLLFVKYGAGKPSIDEKD"
     gene            complement(953..1029)
                     /locus_tag="VIR04_10745"
     tRNA            complement(953..1029)
                     /locus_tag="VIR04_10745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(993..995),aa:Pro,seq:cgg)
     gene            complement(1047..2012)
                     /locus_tag="VIR04_10750"
     CDS             complement(1047..2012)
                     /locus_tag="VIR04_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY9461265.1"
                     /translation="MNLAELIAPVAQDMKAVDAVIRARLNSEVVLIRTIGDYIVGAGG
                     KRTRPALLLMVAGALGYRGVHHHLLAAAVEFIHTATLLHDDVVDESDLRRGLRTANAV
                     FGNAASVLVGDYLYSRSFEMMVETNSMAAMAVLSGATTIIAEGEVLQLMNVRDPEVSL
                     DRYLQVVRYKTAKLFEASTEVGAIIAHATSEQQVAAATYGRHVGTAFQLIDDVLDYSG
                     EALALGKNVGDDLREGKSTMPLIRVLEVGNSQQKQLVRKAIETGKADFAAVAHAIQQT
                     DALQYTRQAAVAEAKLAKQALAAFPVSAYRESLLEFCTFAVERDY"
     gene            2187..3053
                     /locus_tag="VIR04_10755"
     CDS             2187..3053
                     /locus_tag="VIR04_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY9461266.1"
                     /translation="MSATDLTTLISRAERVLAQLEAWLPPAPPEINWSAHAFRWRKRG
                     ARGWLDAVQHVATINVRDLQHIDRQKAIIELNTQHFLHAKPANNVLMTGARGTGKSSL
                     VKAMLAEYGKDGLRLIEVDKTDMGDLGDIVDLVSARPERFIIFCDDLSFEEGEPGYKA
                     LKSVLDGSVSSAGENVLIYATSNRRHLMPEYMSENLSAEHQADGEIHPGETVEEKISL
                     SERFGLWLSFYPFKQDDYLDIVNYWLEVHGCSAADIKASRTEALQWALERGSRSGRVA
                     RQFARDWAARHV"
     gene            3046..>3535
                     /locus_tag="VIR04_10760"
     CDS             3046..>3535
                     /locus_tag="VIR04_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY9461267.1"
                     /translation="MSKPLIDVAAGLIFRPDGKMLLAERPAGKPWAGWWELPGGKIEP
                     GETVLQALSRELKEELNIDVTAATPWVTYTHDYEKRWVRLAFCRVTGWDGMAIGAEGQ
                     KLMWVNPNDPIAVGPLLPATEPPLRWIKIPERYLISSIQSQANLPAFLEKLAQSLENK
                     PTL"
BASE COUNT          845 a         1007 c          967 g          716 t
ORIGIN      
        1 ctacgcatgg ggcaaggcaa gcgacgattt aaatacgatt cgcggcgtgc cgctacccaa
       61 ggaccttaac cagatgacag ggccacaact gtatgacgcc aactgcgcaa gctgtcatca
      121 ggccagaggc cagggcagcc caagcggcgg gctgcccgct ctatttcaca atacggcact
      181 gggtagaacc aatacaaata acctggtcat ggtgctgctg gatgggatag aacggcgccc
      241 cgacgccccc gaagtcctga tgccgggttt cagatcggaa ctgtccgaca ctcaaatcgc
      301 aacgcttggc acatatctga cacaacacta cgggaatcca ggcgcgccgg tcacggtcga
      361 acaagtaaag actttaaggg ccgcaggcca gccctcgtat ttgatctggt tcgcgcgcgc
      421 cggcctggtc attgccggcc tgattatcat tttcttattc ttgttgactc aacgcaggtt
      481 cacgagaagg tccaccgata cacgccaccc aacgcagtta aacaggagaa cataatggac
      541 agattcttga ccatcgccgc acgagtgctc atggcgcagc ttttcattct ttccggctgg
      601 atgaagatca ccggttacag cggtaccgta gggtatttcg ggtcgatggg tttgccgggc
      661 atattggttc cgctcgtaat aattatcgag ctcggaggcg gcctgctttt gctgtttggc
      721 tacaagacca agtgggtggc gattatcctc gcgctattca ctcttgggtc ggcgcttatt
      781 gcccacctgc acctaagcga cccgctgcaa ttcatcaact tcacaaagaa cctcgccatt
      841 accggcggcc tgcttttatt tgtgaaatac ggggccggaa aacccagcat cgacgaaaaa
      901 gattagagaa aacaaaaagc cctgttttta cagggctttt tagcaaacgt gatggtcggg
      961 gcgacatgat ttgaacatgc gactcctgcg tcccgaacgc agtactctac caggctgagc
     1021 tacgccccga cttgtgaaca ctaaacttaa taatcgcgtt ctaccgcgaa agtacaaaat
     1081 tctaacaaag attcccgata agcggaaacc ggaaaagccg ccaaagcctg ttttgcaagc
     1141 ttggcttcgg caacagcagc ctgccgagtg tattgcagcg cgtccgtttg ctgtatggca
     1201 tgcgccaccg ccgcgaaatc ggccttgccg gtctcaatgg ccttgcggac caactgcttt
     1261 tgctggctgt tgcccacttc caggacccgt atcaagggca tggtggattt gccttcacgc
     1321 aaatcatcgc cgacattttt tcccaacgcc agggcctcgc cgctgtagtc gagcacgtca
     1381 tcgatcaact gaaacgcggt cccgacatgc cggccgtaag tcgcggccgc aacctgctgc
     1441 tcggatgtgg cgtgcgcaat aatagcaccg acttcggtcg acgcttcaaa aagcttggcg
     1501 gttttataac gcaccacctg caaataacgg tccagagaca cctcgggatc gcgcacattc
     1561 atcagttgca acacctcgcc ctcggcaatg atggtggtgg cgcccgacaa cacagccatc
     1621 gcagccatcg aattggtctc gaccatcatt tcaaatgagc gtgaatacag gtaatcgccc
     1681 accaaaacgc tggcggcgtt gccaaacacc gcattggcgg tgcgcaggcc gcggcgcaaa
     1741 tcggattcat cgaccacgtc gtcgtgcaaa agggttgcgg tgtgtatgaa ttcaaccgcg
     1801 gcggccagca aatgatgatg cacgccgcga taccccaacg caccggccac catcagcagc
     1861 aaggccggac gggttcgttt gccgccggcc ccgacaatgt aatcgcctat cgtgcgaatc
     1921 aggacgacct cggaattcaa gcgagcgcgg ataacggcat cgacggcttt catatcctga
     1981 gcaacggggg caattaactc ggcaagattc aagacgcgat ccgggtcgga agtaagccaa
     2041 ctggcaaata aacgaagttt acgattatac gttcttgcaa ccgcttcatg cacgacgatt
     2101 gccccgcgca atggagcaga caggaacatg gggcaataaa attggcgata atggcgattt
     2161 gccgcaaatt tactatggag aaaactgtga gtgccaccga tctgactacg cttatcagcc
     2221 gcgccgaacg cgttttggcg caactggaag cgtggcttcc tcccgcgccg cccgaaataa
     2281 actggtcggc gcatgccttt cggtggcgta aacgcggtgc acgcggctgg cttgatgcgg
     2341 tgcagcatgt agcgaccatc aacgtgcgcg acctgcaaca cattgaccgc caaaaagcga
     2401 ttatcgaact taacacgcag cactttctgc acgccaaacc agccaataat gtcttgatga
     2461 ccggcgcgcg cggcacaggc aaaagctcgc tggtgaaggc tatgctggcc gagtacggga
     2521 aggacggcct gcgcctgatt gaagtcgaca aaaccgatat gggcgacctg ggcgatatcg
     2581 tcgacctggt cagcgcccgt cccgaacgct tcattatttt ttgcgacgat ctgtcgttcg
     2641 aagaaggcga acccggctac aaggccctta aatccgtact tgacgggtcg gtcagctcgg
     2701 cgggcgaaaa cgtgctgatt tacgccacgt cgaaccggcg ccatctgatg ccggaataca
     2761 tgagcgaaaa tttaagcgcc gaacatcagg ccgacgggga aatccatccg ggcgaaacgg
     2821 ttgaagaaaa aatttcgctg tccgaacgtt ttggactatg gctgtcgttc tacccattta
     2881 agcaggacga ttatctggat attgtgaatt actggcttga agtacatggc tgctccgccg
     2941 ccgacatcaa ggcgtcgcgc accgaagcat tgcaatgggc gctggaacgt ggttcgcgct
     3001 cgggccgcgt tgcacggcag ttcgcacggg attgggccgc ccggcatgtc taaaccactg
     3061 atcgacgtcg cggccgggct cattttccgg cccgacggca agatgttatt ggccgagcgt
     3121 ccggccggca agccctgggc cggctggtgg gaactgcccg gcggcaaaat tgaaccaggc
     3181 gaaacggtgc tgcaagcgct gtcgcgggaa ctgaaagaag aattgaacat cgacgtgacg
     3241 gccgctacgc cgtgggtcac atacacccac gattatgaaa agcggtgggt aaggctggcc
     3301 ttttgccgcg tgaccggatg ggacggaatg gccatcgggg ccgaagggca aaaattgatg
     3361 tgggtcaatc ccaatgatcc cattgccgtc ggaccgcttt tgcctgccac cgagccgccc
     3421 ctgcgctgga taaaaatacc tgaacgctat ttgatttcgt caatccagag ccaggccaat
     3481 ctacccgctt tccttgaaaa acttgcgcaa tcactggaaa acaagccaac cctgg
//
LOCUS       DATCXU010000483         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1046105,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000483 DATCXU010000000
VERSION     DATCXU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046105"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VIR04_10765"
     CDS             <1..1007
                     /locus_tag="VIR04_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY9461268.1"
                     /translation="TEGVRGEGGILLNKDGERFMERYAPTVKDLASRDIVSRAMDQEI
                     KEGRGCGPDGSYILLKLDHLGAETIKKRLPSIREIAIKFANVDPITDPIPVVPTIHYQ
                     MGGIPAAFDGHVYITENGQDRTVNGLYAIGECSATSVHGANRLGTNSLLDLVVFGRAA
                     GNHIVDSHPERQRAHQSIPQSAIDRSLDRFHKLEDRQSGERVVDVSMAMRNSMQHHCG
                     VFRTPALLAEGVTQIEEIAGRVANISYQDKSKVFNTARIEALELTNMIEVALATTKAA
                     ENRKESRGAHSVSEYPDRDDEKWLVHSLWYSEGRRLDYKPVRLKPLTVDSFPPKPRVY
                     "
     gene            1021..1737
                     /locus_tag="VIR04_10770"
     CDS             1021..1737
                     /locus_tag="VIR04_10770"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY9461269.1"
                     /translation="MTEKRVVKFEIYRYDPDKDERPYMQKLDVELQPGDKMLLDAILR
                     IKNDIDDSLTLRRSCHEGVCGSDAMNINGKNGLACITNLRELTEPIKLRPLPGLPVVR
                     DLVVDMTSFFNQYHSIKPFLINDDPPPEKERLQSPEAREELNGLYECILCGCCSTACP
                     SFWWNPDKFVGPAGLLQAYRFIVDSRDHATEERLDDLEDPYRLFRCHTIMNCVDVCPK
                     KLNPSHAISKIKEMLVRRAI"
     gene            1750..2010
                     /locus_tag="VIR04_10775"
     CDS             1750..2010
                     /locus_tag="VIR04_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY9461270.1"
                     /translation="MKSLSELERARLRWRARRGLLENDLVLTKFLDRYEALLTDVDVS
                     ALTRLFEMGDNELLDVLLARAEPAGDYDTPAIRRLVAQMREL"
     gene            2030..>3074
                     /gene="gltA"
                     /locus_tag="VIR04_10780"
     CDS             2030..>3074
                     /gene="gltA"
                     /locus_tag="VIR04_10780"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY9461271.1"
                     /translation="MHLSDKNATLSFSDGSAPIDLPVYSGTVGPDVIDIRKLYGETGM
                     FTYDPGFLSTAACESSITYINGEKGELLYRGYPIDQLAVNCDYLDICYLILNGDLPNP
                     TQKTEFDSLVTHHTMVHEQLHFFLRGFRRDAHPMAVLTGLVGALSAFYHDSTDITNPH
                     HRDVSAIRLIAKMPTLVAMAYKYSLGQPFIYPQNDMSYTANFLRMMFATPCEDYKVND
                     VVERALDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LQMLEQLQANGGIDKLGEFIAQVKDKHSGVRLMGFGHRVYKNYDPRAKLMQQTCKEVL
                     SALGLEKDPLFKLA"
BASE COUNT          709 a          825 c          846 g          694 t
ORIGIN      
        1 tcaccgaagg cgttcgcggc gaaggcggta ttttgctgaa taaagacggg gaacgcttta
       61 tggagcgtta cgcgcctacc gtcaaggact tggcatcgcg cgatattgtg tcgcgtgcaa
      121 tggatcagga aatcaaggaa ggccgcggct gcggccccga cggcagctac atattgctga
      181 agcttgatca cctgggcgcg gaaacgatta aaaagcgctt gccttcgatt cgtgaaatcg
      241 caatcaagtt cgccaacgtc gaccccatca cggacccgat cccggtggtt ccgactattc
      301 actatcaaat gggcggcatt ccggctgctt tcgatggcca tgtgtacatt accgaaaacg
      361 gccaggaccg gaccgttaat ggactgtatg caatcggcga gtgctcggcc acatcggtgc
      421 acggcgcgaa tcggctgggc accaattcct tgcttgacct ggtggtgttt ggccgcgcag
      481 caggcaatca tatcgttgac agtcaccctg aacgtcagcg cgcgcatcag tcgattccgc
      541 aatccgccat cgatcgttcg ctcgaccggt tccacaagct cgaagatcgg caatcgggcg
      601 aaagggttgt cgacgtctcc atggccatgc gtaattcgat gcagcaccac tgcggcgtgt
      661 ttcgcacacc tgcattgctg gccgaaggcg tcacacagat cgaggaaatc gcaggccggg
      721 tggcgaatat ttcgtaccag gacaaatcca aggttttcaa tacggcccga atcgaagcgc
      781 ttgaactgac caacatgatc gaagtggcgc tggccaccac gaaagcggca gagaatcgaa
      841 aagaaagccg cggtgcgcat tccgtcagcg aataccccga ccgcgatgat gaaaagtggc
      901 tggtccacag cttgtggtac tccgaaggcc gccgcctgga ctacaaaccc gtacggctga
      961 agccgctaac agtagacagc ttcccgccga agccgcgggt ctattaatca ggatatggaa
     1021 atgactgaaa agcgcgttgt gaaatttgaa atttaccgtt atgaccctga caaggacgaa
     1081 cggccttaca tgcagaaact ggatgtcgaa ctgcagcccg gcgacaaaat gctgctggat
     1141 gccattttgc gcataaaaaa cgacattgac gacagcctca cgttgcgtcg ctcatgccat
     1201 gaaggcgtgt gcggctccga tgccatgaat atcaatggga aaaatggcct ggcgtgcata
     1261 acgaacttgc gggaactgac cgagcccatc aagcttcggc ctttgcccgg cttgcctgtc
     1321 gttcgcgacc tggtcgtaga catgaccagc tttttcaacc agtaccattc gatcaagcct
     1381 ttcctgataa acgacgatcc accgccggaa aaagagcgcc tgcaaagccc cgaggcgcgc
     1441 gaagaactca atggtttgta cgaatgcatc ttgtgcgggt gctgttcgac ggcctgtccg
     1501 tctttttggt ggaacccgga caagtttgtc ggacccgccg gcttattgca ggcgtatcgg
     1561 tttattgtcg atagccgcga tcacgccacg gaagaacgtc ttgatgatct ggaagatccg
     1621 taccgcctgt tccgttgcca caccatcatg aactgcgtcg atgtttgccc caagaaactg
     1681 aacccgtcgc atgcgattag taaaattaag gaaatgctgg tccgacgcgc catataagac
     1741 gagcctcaga tgaaatcatt gtcggaactt gaacgagcgc gcctgcgctg gcgcgctcgg
     1801 cgcggtctgt tggaaaacga cctggtcctc accaagtttc tggatcgcta cgaggcattg
     1861 ttgacggacg tcgatgtttc cgctctgact cggttattcg agatgggcga caacgaattg
     1921 cttgatgttt tgctggcccg ggccgaacct gcgggggatt acgatactcc cgcgattcgc
     1981 cgattggttg cgcaaatgcg agagctataa cttttttagg aaaaatgtga tgcatctatc
     2041 agataaaaat gcaacgctat cgttctccga tggcagcgca cccatcgacc ttcccgtgta
     2101 cagcggaacc gttggccccg atgtgattga tattcgcaag ttgtatggcg aaaccggcat
     2161 gttcacgtac gatccgggct ttctgtcgac ggcggcgtgc gagtcgtcca tcacatacat
     2221 taatggcgag aagggcgaat tgctttatcg gggctacccg atcgaccagc ttgccgtgaa
     2281 ctgtgattac ctggatatct gctatttgat tctgaatggc gatttgccca atccgacgca
     2341 aaagactgaa tttgattcat tagtgactca ccacactatg gttcatgaac aacttcattt
     2401 ctttttgcgc ggttttcggc gcgacgccca cccgatggcc gtactgacgg gtttggttgg
     2461 cgcgttgtcg gctttctatc atgactcgac cgacatcacc aatccgcacc atcgcgatgt
     2521 gtcggcaatt cgcctgattg ccaagatgcc gactctggtc gccatggcgt ataagtattc
     2581 attgggccaa ccattcattt acccgcaaaa tgatatgtcg tatacggcta attttttgcg
     2641 tatgatgttt gcaacgcctt gtgaagacta caaggtcaac gatgtcgtgg aacgcgcgct
     2701 agaccggatt ttcattctgc atgccgacca cgagcaaaac gcgtccacat cgacggttcg
     2761 gctgtgcggt tcgtcgggca cgaatccttt cgcggccatt gccgcgggcg tggcgtgctt
     2821 gtgggggcct gcccacggcg gcgccaacga agcctgcctg caaatgctcg aacagttgca
     2881 ggccaatggc gggatcgata agctgggtga attcattgcg caggtcaagg acaaacattc
     2941 aggtgtgcgc ctgatgggct ttgggcatcg ggtttacaag aactacgatc cgcgcgccaa
     3001 gctaatgcag cagacttgca aagaagtcct gtctgcactg gggctcgaaa aagatccttt
     3061 gttcaagctg gcca
//
LOCUS       DATCXU010000484         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_133134,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000484 DATCXU010000000
VERSION     DATCXU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133134"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VIR04_10785"
     CDS             <1..750
                     /locus_tag="VIR04_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY9461272.1"
                     /translation="EQRLSAIFDIARKFKVEPAEIPALQKRLHQQLDANQAAADIQAL
                     STQTDAAKSNYYEAAQRLSAARQKIGRSLAKQVTRAMQTLAMQGGRFEIALHGAEPSA
                     HGNENVEFLVAGHAGTLPRPLAKVASGGELARLSLALSVIASQAARVPTLIFDEVDAG
                     VGGAVAEVVGRLLKELGEQRQVLCVTHLPQVAACAAHHYEVKKSTTDGTTLSAVQALD
                     AQGRVDEVARMLGGLVITDTTRKHAREMLAR"
     gene            complement(747..1169)
                     /gene="fur"
                     /locus_tag="VIR04_10790"
     CDS             complement(747..1169)
                     /gene="fur"
                     /locus_tag="VIR04_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY9461273.1"
                     /translation="MNEQNELKNMGLKATFPRLKILDIFRKADHRHQSAEDIYRALIN
                     EDVDIGLATVYRVLTQFEQAGILVRSQFDGGKAVFELNDGDHHDHLICTNCGKVVEFS
                     DHEIEKRQHKIAKDNAFVLESHTLLLYGICPDCSPKTH"
     gene            1379..1684
                     /locus_tag="VIR04_10795"
                     /pseudo
     CDS             1379..1684
                     /locus_tag="VIR04_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            1768..>2241
                     /gene="dapB"
                     /locus_tag="VIR04_10800"
     CDS             1768..>2241
                     /gene="dapB"
                     /locus_tag="VIR04_10800"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY9461274.1"
                     /translation="MRIAIAGADGRMGRMLIEAVANSEDLNLTVALGRAGTPIIGQDA
                     GAFLGKKTDVIVTDNLDTLAQADCLIDFTRPEGTLAHLSACVRHGTKCVIGTTGFSDS
                     EKQIIHAASQKIAIVFAPNMSVGVNVALTLLDMAAKLLSSGYDVEIFEAHHRNKVD"
BASE COUNT          542 a          649 c          591 g          459 t
ORIGIN      
        1 gagcagcgac tgtcggccat cttcgacatt gcgcgcaaat tcaaggtcga accggccgaa
       61 attcccgccc tccagaaacg tttgcatcag cagcttgacg ccaaccaggc cgccgccgac
      121 atccaggccc tctcgacaca aaccgatgcc gcaaaaagca attattacga agcggcgcaa
      181 cgactaagcg cagcccggca aaagatcgga agatccctgg caaaacaggt tactcgcgcc
      241 atgcagacac tggccatgca aggggggcgc ttcgaaattg ccctgcatgg agccgagcca
      301 tccgctcacg gcaacgagaa cgtcgagttt ttggtggccg gccatgccgg aacgcttccc
      361 aggccactgg ccaaagtcgc ctcgggggga gaactggcgc ggctttcatt ggcgctatcg
      421 gtcatcgcca gccaggcggc acgtgtgccc accctgatat tcgacgaggt cgatgccggc
      481 gtgggagggg ccgtggccga agtggtgggc cgtctgttaa aagaactggg cgaacagcgc
      541 caggtattgt gcgtgactca cttgccgcaa gtggccgcgt gcgcggcgca ccattatgaa
      601 gtcaaaaaat ccacaaccga cggaaccacc ctgtcggcgg tgcaagcact ggacgcgcag
      661 ggacgcgtgg acgaagtcgc gcgcatgctg ggcggcctgg tcattaccga caccacgcgc
      721 aaacacgcac gcgagatgct ggcccgttaa tgcgtcttgg gcgaacagtc gggacaaatt
      781 ccatacagca gcaaagtgtg gctctcaagc acaaatgcat tgtctttggc aattttgtgc
      841 tggcgctttt cgatctcgtg gtctgaaaac tcgaccactt ttccgcaatt agtgcaaatc
      901 agatggtcgt gatgatcgcc gtcgttcagc tcaaacaccg ccttgccgcc gtcgaattga
      961 ctacgtacta aaatgcctgc ttgctcgaat tgcgttaaca cccgatacac cgttgccagg
     1021 ccgatgtcga catcttcatt gatcaacgcg cggtatatgt cttcggcgct ttgatgacga
     1081 tgatcggctt tacgaaaaat atcgagaatt ttcaggcgag gaaaagtcgc cttcaatccc
     1141 atatttttta gttcattttg ctcattcata gttaaagctt ctctggcgcg taaaaaataa
     1201 tgtcggatgc tttcaccatc agaaaacatc caatcgtgac taacgctatt atgcgtttgt
     1261 tcttatacct ttatgataac gttttattcg acttgaatat caggggcgtt tcgtgctgac
     1321 gactgtaaaa ccactacttt acaaactgcg gactgcaata tcgataagcg cgctggctat
     1381 ggcgctatcc gcctgcggca cgaccaattg gggatttcct taccgggccg gagtccagca
     1441 gggcaactgg attaccgccg aacaggttgc gcaacttcac aaaggcatga cgcgcgatca
     1501 ggtgcgtttc gttcttggca cccccacctt gcaggatgta tttcaaagcg accgatggga
     1561 ttatccctac tttaacaaac cgggatatgg caaacaacaa gagcgcaaat tcaccgtctg
     1621 gttccagggc gatagccttg atcattggga cggagatccg caacctgacc ggcagccttt
     1681 ccagaaggcc gacaccggca tgcatgaacc aaccgccaag cccgcttcaa actcaacgga
     1741 accccaaact tctcgccagg aaaaataatg cgcatcgcta tcgccggagc tgacggccgc
     1801 atgggccgaa tgctgatcga ggccgtcgcc aacagcgagg atctgaatct tacggtcgcg
     1861 ctgggtcgcg ctggaacacc catcatcggg caggacgcgg gtgcgtttct aggcaaaaaa
     1921 acagacgtta ttgtcaccga caatctggac acgctggcgc aagccgactg cctaatcgac
     1981 tttacccgcc ccgagggaac gctggcccac ctcagcgcct gcgtccggca cggcaccaag
     2041 tgcgtcatag gcacgaccgg attcagcgat tcggaaaaac agatcatcca cgccgccagc
     2101 caaaagatcg ccatcgtttt cgcgcccaac atgagcgttg gcgtaaatgt cgctctcacg
     2161 cttctggaca tggccgccaa gcttttaagc agcggctacg atgttgaaat cttcgaggcg
     2221 caccatcgca acaaagtcga t
//
LOCUS       DATCXU010000485         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_508178,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000485 DATCXU010000000
VERSION     DATCXU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508178"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VIR04_10805"
     CDS             complement(<1..1419)
                     /locus_tag="VIR04_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY9461275.1"
                     /translation="MQGNSLLLLTLVYLAAGVISVPIVQRLGLGSVLGYLIAGAIIGP
                     FALHLVGDQTDVMRFAEFGIVIMLFLIGLEVRPSLLWGMRRAFFGLGGTQVIGTGMVL
                     TAVGTAFGLDWRAAAALGFILALSSTAIVLQTFEEKGLTHTAAGKAAFSILLFQDVST
                     IPMFAALPLLAVGAAATLAQTPNHSFLAGFPAWAQAFAVLAAVMLVIGTGRYLTRPAF
                     RFIADTHSREIFTAAALLLVVAVALLMEAVGLSPALGAFLAGVVLADSEFRHELEVDI
                     EPFRGLLLGLFFISIGASMNLGLLISRPVALISFVAGLMAAKMAVVYVVARAFHVERR
                     AAALTATALAQGGEFAFVLLGIASASGIITADLSELATAAVAISMALTPLMLIAYKRW
                     CSDDLAKGPEPRNDAFDNRPDVIVAGFGRFGQIAARLLIVNRFKISTLDASAEQVDLV
                     RRFGSRVYYGDATRLDLLRAAGA"
     gene            complement(1449..2345)
                     /locus_tag="VIR04_10810"
     CDS             complement(1449..2345)
                     /locus_tag="VIR04_10810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY9461276.1"
                     /translation="MITERKKASDFPPAVLKLFDSYVHGMIDRRQFLNRAAKFAVGGF
                     TASAMLESLKPNYAFAQHVPKDDARIKTAYLDYPSPRGSGTMRGYLALPANAAGKLPG
                     VLVIHENRGLNPYIEDVARRLALENFVAFAPDALTPLGGYPGDEEKAVKLFAQLDPKK
                     RLQDFVAGTEFLKSRPECTGKVGAVGFCFGGGVANSLAVLLPDLGAAVPFYGNQPSAA
                     DTAKIKAPLLIHYAGLDERITSGWPAFESALKANGVRYREYTYPGVNHGFHNDTTPRY
                     DEAAAKLAWSRTVAFLNKHLRS"
     gene            2410..3066
                     /locus_tag="VIR04_10815"
     CDS             2410..3066
                     /locus_tag="VIR04_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY9461277.1"
                     /translation="MQPARHFFHTHRLLIIAIVTGTGIALALPPDWNFADRVLSGWNI
                     TVWFYLCVTGWLMMRAGEARVRKLAEQEDESAGAVLAIMSVASVASLVAIVMELSTVN
                     DLSGGVRLLHYAFTGATVLGSWFLLGVLFTHHYARQFYRSPPHQRSLRFPDEEQKPDY
                     WDFLYFSFTIAVAVQTSDVLIMSRSVRRSAIIQSVLSFWFNVAIVGLSINIAGSLIGS
                     "
     gene            complement(3078..>3751)
                     /locus_tag="VIR04_10820"
     CDS             complement(3078..>3751)
                     /locus_tag="VIR04_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEY9461278.1"
                     /translation="AALAILTSGAWVFNYRSTADLAALTAQTPQHVITLDQWTNTAWR
                     DLPQRRVELGGDNEELFLLQWAESRNGLQSRLKSAGWSVPPVWSVKTALLWLSPNAAA
                     GDLPVLPKYSHGKSSRLAFVRLDPAYPQQRIVLRLWHSDYLLQNSDPPAATPIWYGAF
                     YEESLKRPWHLFTLGIAKNLTDGSLFTRLLPADLDAVVRRHAPNASSVHTVLALPQRE
                     PSSGH"
BASE COUNT          810 a         1046 c         1116 g          779 t
ORIGIN      
        1 tgcgccagcg gcgcgcagca agtcgagccg cgtggcatcg ccataataaa cgcggctccc
       61 gaagcggcgc accagatcga cctgttcagc actggcgtcc agcgtcgaaa ttttgaaacg
      121 gttgacgatc aacagccgcg cggcaatttg gccaaaacgg ccaaagccgg ccacaatgac
      181 atcaggacga ttgtcgaagg cgtcattccg gggttcgggg cctttcgcca ggtcgtccga
      241 gcaccagcgc ttgtaggcga tcagcattag cggggtgagc gccatggaaa tggccacagc
      301 ggcggtcgcc agctcggaaa gatccgctgt aataatgccc gacgccgagg caatgcccag
      361 caacacaaag gcgaattcgc cgccttgtgc cagcgcggta gccgttagcg ccgctgccct
      421 gcgttcaaca tggaaggcgc gtgcgacgac gtagacgacg gccattttgg cggccatcag
      481 acccgcaacg aacgagatca gcgcaacggg ccttgaaatc agcaggccca aattcatgct
      541 ggcgcctatg gaaatgaaaa acagcccaag cagcaggccc cggaatggct cgatatcgac
      601 ttccaattcg tgtctgaatt cgctgtcggc cagaacgacg ccggccaaaa atgcgcccag
      661 tgcgggcgat aggcccacgg cttccattaa caaggccacg gcaaccacca gcagaagagc
      721 tgcggcggtg aatatttcgc gtgagtgcgt gtcagcaata aaacggaaag ccggccgtgt
      781 caaatagcgg cccgttccga ttacaagcat gaccgcggca agaacagcaa aggcctgcgc
      841 ccaggcgggg aatccagcca aaaagctgtg gttcggcgtt tgcgccagcg ttgcggctgc
      901 gcccaccgca agcagcggca acgccgcgaa catcgggatg gtagagacgt cctggaataa
      961 caagatgctg aacgccgcct tgccggcggc tgtgtgagta agcccttttt cttcgaaggt
     1021 ttgcagaacg atcgcggtgg atgacagcgc cagaatgaac cccagcgccg cagccgcacg
     1081 ccaatccagt ccgaaggctg tgccaactgc tgtcaggacc atgcccgtgc ctatcacctg
     1141 ggttccgccc agaccgaaga aggcccggcg catgccccat agaagggatg ggcgcacctc
     1201 caggccgatc aaaaacagca tgatgacgat gccgaactcg gcaaagcgca ttacgtccgt
     1261 ctggtcgccc accagatgca aggcgaaggg accgatgatg gcgccggcaa tcagatagcc
     1321 cagcaccgag ccgagcccca gtcgctgcac gattggaacc gagattacac cggcggccag
     1381 atagaccagc gtcagcagta gcaggctgtt gccttgcaag ggatatcttc cttattgaaa
     1441 aacttggttc aggatcgaag gtgtttgttc aggaatgcta cggtgcgtga ccacgccagc
     1501 tttgccgccg cctcgtcgta gcgcggggtc gtatcgttat gaaagccgtg attgacgccc
     1561 gggtacgtat attcgcgata gcgtactccg ttggccttga gtgcgctttc gaaggcgggc
     1621 cagccggacg tgatgcgctc gtccagcccg gcatagtgga tcagcaaagg cgccttgatt
     1681 ttggctgtgt cggcggcact gggctggttg ccgtagaatg ggacggcggc ccccagatcg
     1741 ggcaaaagca cggccagact gttggcgacc cctccgccaa agcagaatcc gaccgcgccg
     1801 accttgcctg tgcattcggg ccgcgatttc aaaaattcgg tgccggcaac gaagtcttga
     1861 agacgctttt tcgggtcaag ctgggcaaat agcttgacgg ctttctcctc gtctcccggg
     1921 taaccgccca atggcgtaag cgcgtcgggt gcgaaggcga caaaattttc cagcgccagg
     1981 cggcgggcaa cgtcttcaat gtaggggtta agtccgcgat tttcatgaat gaccagtacg
     2041 ccgggtaatt tgcccgcggc atttgcgggc aatgcgagat acccgcgcat agtgccggag
     2101 ccccgtggcg acggataatc cagataggcg gttttgatgc gcgcatcgtc ctttgggaca
     2161 tgctgcgcga aggcatagtt tggtttgagg ctttccagca tggccgaggc ggtgaagccg
     2221 cccaccgcga atttggcggc gcgattcagg aattgacggc ggtctatcat gccgtgcacg
     2281 taagaatcaa acaacttaag gacggcagga ggaaaatcgc tggctttttt gcgttctgtg
     2341 atcataatta ggctcctttg gcgcgacatg tcctatgtac aataatttat ctgaatagtc
     2401 acttcaaata tgcaaccagc gcgccatttt tttcatactc atcggctact gatcattgcc
     2461 attgttacgg gaacaggcat tgcgctggcg cttcccccgg actggaattt cgctgaccgg
     2521 gttttgtccg gctggaatat aacggtgtgg ttctatctgt gcgtgacagg atggctgatg
     2581 atgcgcgctg gcgaggcccg ggtgcgcaaa ctggctgagc aagaggacga aagcgcgggc
     2641 gcagttcttg caattatgtc ggtcgcgtcg gtggcaagtc tggttgcaat cgttatggag
     2701 ctatcgaccg tcaacgattt gtccggcggc gtgcgcttgt tgcactatgc ctttacgggc
     2761 gccactgttc tcggttcctg gtttctgctg ggcgtcttgt tcacccacca ttatgcacgt
     2821 cagttctacc ggtcgccgcc gcaccagcgg tctttgcgtt ttcccgatga agaacaaaaa
     2881 cccgattatt gggacttcct ttatttttcg tttacgattg ccgtagcggt tcaaacttcg
     2941 gacgtgctca taatgtcaag atcggtgcgc cggtcggcaa taattcagtc cgtcctcagt
     3001 ttttggttca atgttgccat cgtaggcctg tccatcaata ttgcgggcag cctgatcgga
     3061 tcgtgatcaa agcttaatta atggcctgag ctcggttcgc gttgcggcaa tgccagcacg
     3121 gtatgcaccg atgatgcgtt cggcgcgtgg cggcgaacca cggcgtccag atcggcaggc
     3181 agcagacgcg taaataacga tccatctgtc aggtttttag cgatacccag cgtaaacaaa
     3241 tgccaaggcc gcttgagact ttcttcataa aaagcgccgt accagatggg tgtcgcggct
     3301 ggcggatctg agttttgcaa caggtaatcc gagtgccaca accggagcac gatgcgttgt
     3361 tgcggataag ccggatccag tctgacgaaa gccaatctcg agcttttacc gtgactgtat
     3421 tttggaagca cgggcagatc gccggctgcg gcattgggcg agagccacaa tagtgccgtt
     3481 ttaacggacc agacgggcgg gaccgaccat cctgcgcttt ttagccggct ctgcaacccg
     3541 ttccggcttt ccgcccattg cagaagaaac aactcttcgt tgtcgccgcc aagttcaact
     3601 cgtcgttgcg gcaggtcgcg ccatgcggta ttcgtccatt gatccaatgt gataacgtgc
     3661 tgcggtgttt gtgcggtgag ggccgcgagg tcggccgttg aacggtaatt gaaaacccac
     3721 gccccgctgg tcagtattgc caaggcggca a
//
LOCUS       DATCXU010000486         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_842453,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000486 DATCXU010000000
VERSION     DATCXU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842453"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VIR04_10825"
     CDS             complement(<1..530)
                     /locus_tag="VIR04_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEY9461279.1"
                     /translation="MRRHWLFAGLLLLLLHAAAGAQGNLSRLLNHPAPGGVAVVTLGN
                     TAAAPAVTYLGHKVMVVRDSDGQWVAVLGIGLKTPVGPAELQAVDARGVRRIAFAIGH
                     RSYKEQHIVLKNRSQVNPDPAQLERFDREYKEQMAAYNQFRNIVPSNLVLDRPVPGRL
                     SSPFGLRRFFNGEERNP"
     gene            complement(577..1248)
                     /locus_tag="VIR04_10830"
     CDS             complement(577..1248)
                     /locus_tag="VIR04_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461280.1"
                     /translation="MDYTHSIEVNELCQRVSDSAGELSILDGVSFTIDPGEAVAVTGS
                     SGSGKSTLLGLLAGLDIPTSGQVKLLGRDLFSLDEEQRAAVRAAHVGFVFQSFHLLPN
                     LTALENVMLPLELARKPALSTAKGILERVGLKDRLFHYPKTLSGGEQQRVSLARAFVV
                     QPELLFADEPTGSLDEQNGAKIIDLMFSMQREQGATLVLVTHDSRLASLCQRELKIHG
                     GRIVS"
     gene            1247..1888
                     /locus_tag="VIR04_10835"
     CDS             1247..1888
                     /locus_tag="VIR04_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY9461281.1"
                     /translation="MLIHWRLIVALLCWFPLSGVQAGEQAQTEPASTILVMGDSLSAE
                     YGLERGTGWVSLIAKKLSEGKHAYRVQNSSISGDTTAGGVTRLPDALKQYSPGIVVIE
                     LGSNDALRGLSLSATESNLAKMIQLIQQSGAQAILVGMEIPPNYGREYTSQFKQIFTT
                     LAQRHHVRLVPFLLKGIATDRQQFQADGIHPNEAAQPALAQNVWAELEPALKQ"
     gene            complement(1895..3856)
                     /locus_tag="VIR04_10840"
     CDS             complement(1895..3856)
                     /locus_tag="VIR04_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY9461282.1"
                     /translation="MFEFVRTHQRLMQLILLVLIVPSFALIGVGGYSTYVSGDHDLVK
                     VGDSAITLQQFDEARRNQLQQMQQDNPGGFDPAALDTKEARSALLDTLIDRTVLVAAA
                     AKDRFSVSDAILRQTIASMPELQVEGKFSAQRYDELLKSNGLTTKGFEQSRRADLALS
                     RVLGPVAATASVPEPVVNSIEQVLSAQRTIRVLGFPSSDYSKGIDIAASDIQAWYDKN
                     KKELELPEQVSARYLLLNEAAAMQNLPAISHDAMLKYYEQNKARYVVPGRVNLSHILV
                     TVAAGATDAQRKIALAKADQIAKKVSANVDQFAEIARTESQDAGTARNGGKLGWITHG
                     SWPAPIEKAVFALKKGEVSGVVEGPEGYHIFKADDIQPQTGETFEQAKSKVESEIRRQ
                     MGADRFADMATKLTSLVYDNSSSLEPASQALGIKVKTANGIARDHLLAANEVGADAAS
                     ASADATVLNDARVRRALFAPQALTEKQNSGVIEISPDTMIVVRVSNVVPAHIPPLDKV
                     TDLIRQRLVAERALTAAADAGKQALAAFQKESSFKAPDKFGAANTISRANPQGVSGLV
                     LDAIFKASVKTLPTYVGIRAAQGYVVVRIEQAQPGKTNDPSLATLPAELNQAWGQAEQ
                     EAVLKTLRTQAKVKMLPEASKALAGEAAS"
     gene            4016..4768
                     /gene="ugpQ"
                     /locus_tag="VIR04_10845"
     CDS             4016..4768
                     /gene="ugpQ"
                     /locus_tag="VIR04_10845"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY9461283.1"
                     /translation="MPNPPASWPYPKLIAHRGAGRFAPENTLASMRLGAKHGFTMVEY
                     DVKLTLDGVAVLLHDDLVNRTSNGTGNAAELSFGELAKLDFGAWHSNEFAGEPIPTLY
                     AIAAFTQANGILSNIEIKPHTGADVVTGTEVARLARQLWAHAETPPLLSSFSEQALYA
                     AFQHAPELPRALLIERELPDDLESRLTRLGCTGLNINTKFASRQTIEWAHKSGYKIGI
                     WTVNDPRRARELLDWGGDSIFTDEIKLIAPHS"
     gene            4790..5674
                     /gene="rlmJ"
                     /locus_tag="VIR04_10850"
     CDS             4790..5674
                     /gene="rlmJ"
                     /locus_tag="VIR04_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY9461284.1"
                     /translation="MFSYRHAFHAGNHADVLKHAIFVHILDYFNQKDAPYWVIDTHAG
                     AGVYDLTSEWALKNGEFIDGLDRLLDTDSKPPMIMRYLKEIQYANPDGAANLYPGSPW
                     LALHALRGRDRLRLFELHPTEAQVLKQNLTRQGRGALRKTAIYEEDGFQGLKKLLPPP
                     SRRAITIIDPSYENKRDYRATLQAVNDGLERFATGCFMIWLPLVQRIEAHELARALER
                     LKTHTWVHASLTVRKPASDGVGLHGSSVFVINPPWTLHADLEKTLPWLSQKMAMDSRA
                     AFTLTQYSPGPEHKRRPA"
     gene            complement(5686..6420)
                     /locus_tag="VIR04_10855"
     CDS             complement(5686..6420)
                     /locus_tag="VIR04_10855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY9461285.1"
                     /translation="MEKTRISKLMAERGMCSRREADAYIERGWVRVDGVIVSQLGSKA
                     FPDQKITLEKQARQRQMSRVTILLNKPVGYVSGQAEQGYRPAIVLISAATQYHSDKSA
                     AQFDPNHLRGLAPAGRLDIDSQGLLVLTQDGRIARQLISDDSDIDKEYLVRVQGKIAG
                     NGLALLNHGLTLDGVLLKPARVSWQNEDQLRFILHEGKKRQIRRMCEQVGLKVTGLKR
                     VRMGRIVLGDLPPGKWRYLKNTEHFL"
     gene            complement(6501..7235)
                     /locus_tag="VIR04_10860"
     CDS             complement(6501..7235)
                     /locus_tag="VIR04_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY9461286.1"
                     /translation="MIEFKNVSKWYGSFQVLKDCSTSVATGEVVVVCGPSGSGKSTLI
                     KTANALEPFQKGEIWIDGISVGAPETNLPKLRSRIGMVFQHFELFPHLSVMRNLALAQ
                     VKVLGRPEKEALERGRTLLERVGLSDQTDKFPGQLSGGQQQRVAIARALAMDPVAMLF
                     DEPTSALDPEMVNEVLDVMVDLAKEGMTMMVVTHEMGFAKKVADRVVFMDSGTIVEDC
                     SKDDFFDHVDQRSDRAKLFLSKILSH"
     gene            complement(7253..>7550)
                     /locus_tag="VIR04_10865"
     CDS             complement(7253..>7550)
                     /locus_tag="VIR04_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9461287.1"
                     /translation="AGYALGLNYWQVMGHIVLPQAFRNMTPVLLTQMIVLFQDTSLVY
                     VLSLTDFLGAANNVAQRDGRLVEMFIFAAVIYFLISYGASRTVRILEQRMAIVR"
BASE COUNT         1733 a         2284 c         1950 g         1583 t
ORIGIN      
        1 ggattgcgct cctcgccgtt gaaaaaacgg cgtaagccaa aagggctgga cagacggcca
       61 ggaacgggcc tgtccagcac gagattgctg gggacaatat tgcgaaactg gttgtaggcc
      121 gccatctgct ctttgtattc ccggtcgaaa cgttcaagct gcgccggatc cggattaacc
      181 tggcttctat ttttaaggac aatatgctgc tctttatacg acctgtgtcc tatggcgaaa
      241 gctatacgac gcacgccgcg cgcatcaaca gcttgcaatt cggccggacc gaccggcgtt
      301 ttcagcccga ttccaagcac ggcaacccac tgcccgtcac tgtcgcgaac aaccatgacc
      361 ttgtgcccca aataggtgac tgccggcgcc gcagccgtat tgcccagcgt caccaccgca
      421 acgccgcccg gcgccgggtg gttcagcaaa cggctcaagt tgccttgtgc gcctgccgcc
      481 gcatgcaaaa gcaaaagcaa taaacccgca aataaccaat gtcttctcat gatttcctga
      541 tcaaatcaac gttcacccgc agcgcccgcg cattgttcac gatacgatgc gcccgccgtg
      601 aattttcagt tcacgctgac acaacgaagc caggcgtgaa tcgtgcgtca ccaacaccaa
      661 ggtcgctcct tgctcgcgtt gcatggaaaa catcaggtcg ataattttcg cgccattttg
      721 ttcatcaaga ctgccggtag gttcgtccgc gaacagaagc tctggctgca ccacgaaggc
      781 tcgcgccagc gatacgcgct gctgttctcc gccggaaagc gtcttcgggt aatggaacag
      841 gcgatctttc aggcccactc tctccagaat gcccttcgca gtcgacaaag caggtttgcg
      901 cgccaactcg agcggcaaca tcacattctc caacgcagtc agattaggca ataaatgaaa
      961 cgattgaaaa acaaacccga catgcgccgc ccggacagcc gcccgctgct cttcatcaag
     1021 agaaaacagg tctcgaccca gcagcttgac ctgacccgag gtcggaatat cgagccccgc
     1081 cagaagcccc agcagagtgg atttccccga ccccgagctg cccgttaccg ctaccgcttc
     1141 acccgggtca attgtgaagc tgaccccgtc caggatgcta agctcgccgg cagagtcgct
     1201 tacccgctgg cataattcat ttacttctat cgaatgggtg taatccatgc tgattcactg
     1261 gcgtcttata gtagctctat tatgctggtt tccattgtcc ggcgttcagg ccggggaaca
     1321 ggcccagact gagccggcct ccaccattct ggtcatgggc gatagcctgt cggccgagta
     1381 cggtctggaa cgcgggaccg gctgggtctc ccttattgcc aaaaagttaa gcgaagggaa
     1441 acacgcctac cgcgtgcaaa attccagtat cagcggcgac acgacagccg gaggcgtaac
     1501 ccgcctgcca gatgcattaa aacaatattc acccggcatt gtcgtgatcg agctgggcag
     1561 caacgacgcc ttgcgcggct tgtcattaag cgcgaccgaa tccaacctcg cgaaaatgat
     1621 tcaactgatc cagcaatcgg gcgcgcaagc gatcttggtc ggcatggaaa ttccacccaa
     1681 ttacggccgc gaatatacgt cacaattcaa gcagattttc accacgctgg cgcagcggca
     1741 ccatgtacga ctggtcccat ttctactaaa aggtattgcc acggatcggc agcaattcca
     1801 ggctgacgga atccatccaa acgaagcggc gcaaccggcg ctggcgcaaa acgtgtgggc
     1861 cgaactggaa ccggccctta aacagtgacg cgccttacga ggcggcttca ccagccagcg
     1921 ctttgctggc ctcgggcaac attttcacct tggcctgcgt gcgcaatgtt tttaatacgg
     1981 cttcttgctc ggcctgcccc cacgcctgat tcaattccgc aggtagcgta gccagcgagg
     2041 ggtcattcgt cttgcccggt tgtgcctgct cgatgcgcac aacgacatac ccctgagccg
     2101 cgcggatacc cacataggtc ggcaacgtct tcacggacgc tttaaaaatg gcgtcgagca
     2161 ccaaaccact taccccttgc gggtttgcgc ggctaatcgt gttcgcggcg ccgaatttgt
     2221 ccggcgcctt gaagctgctt tctttttgga atgcggccaa cgcttgtttg ccagcgtctg
     2281 cggccgccgt caaagcgcgt tcggcgacaa ggcgctggcg tataaggtcc gtgaccttgt
     2341 caagaggggg aatatgggcg ggcacgacgt tgctaacacg caccacgatc attgtgtcgg
     2401 gtgaaatttc aatgactccc gagttctgct tttcagtgag cgcttgcggg gcaaacaagg
     2461 cgcgacgcac ccgtgcatcg ttaagcacgg tcgcatcggc gctggccgag gccgcatcgg
     2521 cgcccacttc attggcggca agcaaatgat cgcgcgcaat gccattcgcg gttttgacct
     2581 tgattcccag cgcctgggac gcgggctcca ggctgctgga attgtcgtaa acgaggctcg
     2641 tcagcttggt ggccatgtcg gcaaagcggt cagcgcccat ctgccggcga atttcggatt
     2701 cgaccttgct ctttgcctgc tcgaatgttt cacccgtctg gggttgaata tcgtccgcct
     2761 tgaaaatatg gtaaccctcg ggcccttcaa caaccccgga gacctcgccc tttttcaaag
     2821 cgaagacagc tttctcgatg ggagcaggcc aggaaccatg tgtaatccag cccagtttcc
     2881 cgccattcct ggcagtgccc gcatcctgcg actcggtgcg agcgatttcg gcgaactggt
     2941 ccacattggc tgacaccttc ttggcgatct gatcggcctt cgcaagcgca atcttgcgct
     3001 gcgcatcggt tgccccagca gctacggtga caagaatatg actaagattg acgcgcccgg
     3061 gaacaacata gcgcgccttg ttctgctcgt aatatttaag cattgcatcg tggctgattg
     3121 ccggcagatt ctgcatcgcc gccgcctcat tcagcagcag gtagcgcgcg ctgacctgtt
     3181 cggggagttc aagctctttt ttgttcttgt cgtaccaggc ctgaatatcg gacgcggcaa
     3241 tatcaatgcc tttgctatag tccgaggatg gaaaccccag cacgcgtatc gtgcgctgcg
     3301 cactaagcac ctgctcgatt gaattaacca caggttccgg aacgctggcg gtggccgcca
     3361 cggggccgag cacgcggctt aacgccaaat cggcgcgacg gctctgctcg aatcctttgg
     3421 tggtcaggcc attagacttc aaaagctcat catagcgttg cgcagaaaat tttccttcca
     3481 cctgaagttc aggcatggaa gcaattgtct gacgcagaat cgcatctgaa accgagaacc
     3541 ggtctttcgc cgcagccgca accaatacag tgcgatcgat cagcgtatcc agcaaagcgc
     3601 tacgcgcctc tttggtgtcc agtgccgcag ggtcaaagcc gcccgggttg tcctgctgca
     3661 tttgctgcaa ctggttacgc cgtgcctcgt cgaattgctg taaggtaatg gccgagtcgc
     3721 cgaccttgac caagtcgtgg tcgcccgaca cataggtgga atagccgccc acgccaatca
     3781 gggcgaatga cggaacaata agcaccaaca aaatcaactg catcaagcgt tgatgcgtgc
     3841 gaacaaattc gaacattgat tgataactcg ctgactcgcc ggcctgttaa agctttatgc
     3901 ggcggtttat actaaaaaaa gcattgaaaa gcgtgccttg gccgtctgcc gcgtttgtgc
     3961 ggaacacgaa aaaatttctt ggcagtttac cacctctgga atccaaatct gaaaaatgcc
     4021 gaacccgcct gcttcgtggc cgtatccgaa actgatcgca caccgaggcg ccggccggtt
     4081 tgcccctgaa aacaccctgg catccatgcg actgggcgcc aaacatggtt ttaccatggt
     4141 tgagtatgat gtaaaactga ctttggacgg cgtagcggtg ctgctgcacg acgatctggt
     4201 caatcgcacg tcgaatggca caggcaacgc cgccgaactg tcctttggcg aactcgcgaa
     4261 acttgatttc ggcgcctggc attcaaacga attcgctggc gaaccgattc ccactttata
     4321 cgcgatagcc gcctttacgc aagccaacgg cattttgagc aatattgaaa ttaagccgca
     4381 caccggcgct gacgtcgtca cgggaacaga agtagcgcga ctggcgcggc aactctgggc
     4441 ccatgctgaa accccgcccc ttttatcgtc cttttccgag caggcgcttt atgccgcctt
     4501 tcaacacgcg cccgaactgc cgcgcgctct gctcatcgaa cgcgaattgc ctgacgacct
     4561 ggaatcccgc ctgacccggc ttggctgcac cgggctgaac ataaatacca aattcgcttc
     4621 cagacaaacc attgaatggg cgcacaaatc aggttataaa attggcatct ggacggtaaa
     4681 cgatccaagg cgcgcgcgcg aactgcttga ttggggcggc gactcaatct tcaccgatga
     4741 aataaaactc atcgctccac attcataatt tcgccacgaa aggccgcaat tgttcagcta
     4801 ccgtcacgct ttccacgcgg gtaatcatgc cgacgttctc aagcacgcca tctttgttca
     4861 tatcctggat tacttcaatc agaaagacgc tccttactgg gttatcgaca cgcacgcggg
     4921 cgcgggcgtt tacgacctga ccagcgaatg ggccctcaag aacggcgagt tcattgacgg
     4981 gctggaccgg ttattggaca ccgattcaaa gccgccgatg atcatgcgct accttaaaga
     5041 aatacaatac gccaacccgg atggggcggc aaatctctat ccaggatccc catggctggc
     5101 cctgcatgca ttgcgcgggc gagatcgcct gcgactattt gaactgcatc cgaccgaagc
     5161 ccaggttctg aaacaaaacc tgacgcgaca aggccgcggc gcgctgcgca aaacggcaat
     5221 ttacgaagag gatggattcc agggcttgaa aaagctgctt ccgcccccgt cgcgacgggc
     5281 catcacaatc atagacccct cgtacgaaaa taaacgggac taccgggcca ctctgcaagc
     5341 agtgaacgac ggcctggaac gtttcgccac cggctgcttc atgatctggt tgccattggt
     5401 gcaaagaatc gaagcgcacg agctggcccg agcgctcgag cgcctcaaaa cacacacatg
     5461 ggtgcacgca agcctgaccg tgcgcaagcc tgcctcggat ggcgtcggcc tgcatggaag
     5521 cagcgtgttc gtcatcaatc cgccatggac cctgcatgcc gatctggaga aaacgcttcc
     5581 atggcttagc caaaaaatgg ccatggatag ccgcgcggcc ttcacgttga cgcagtattc
     5641 acctggacct gaacataagc gcaggcccgc ttgaaccacg gtccgctaca aaaaatgttc
     5701 cgtgttcttt aaataccgcc attttcccgg cggcagatcg cccaaaacaa tgcgccccat
     5761 ccgcaccctc ttgagtcctg tcacctttag gccgacctgc tcgcacatgc gtcgaatttg
     5821 ccgtttcttg ccttcatgca aaatgaatcg aagctgatcc tcgttttgcc agcttacccg
     5881 cgcgggcttg agcaaaactc cgtccagcgt cagaccatgg ttaagcaagg ccagaccgtt
     5941 gcccgcaatt ttcccctgca cacgcaccag gtattccttg tcgatgtcgg aatcatcgga
     6001 aatcaattgg cgtgcgattc gcccgtcttg agtcagcacc agcaaaccct gagagtcaat
     6061 atccagccgg ccggcgggag ccagaccgcg caaatgattc ggatcgaatt gcgcggcgga
     6121 tttgtcgctg tgatactgcg tagcggcgct aatcagcacg atcgccgggc gatagccctg
     6181 ctcggcctgc cccgacacat agcccaccgg cttattcagc agaatcgtaa cgcgcgacat
     6241 ctggcgttgg cgcgcctgtt tttccagcgt tattttttga tcggggaacg ccttgctgcc
     6301 aagctgggac acaatcaccc cgtcgacccg aacccagccc cgctcgatat aagcgtcagc
     6361 ctctcggcgc gaacacatgc cgcgctcggc catcaatttc gaaatccgcg ttttttccat
     6421 aagcggcatt gtaaatgctt gccggcatta tcgctgacgg accatgggcc aacccggacc
     6481 cgtcgccggg cctcactctg tcaatgagat agaattttcg acaggaacaa cttggcgcga
     6541 tcgcttcgct gatcaacatg gtcgaagaag tcatctttcg aacaatcttc cacaatcgtg
     6601 cccgaatcca tgaagacgac acggtcggcg accttcttgg caaaccccat ttcatgggta
     6661 acgaccatca tggtcatgcc ttctttggca aggtccacca tgacatccag cacctcgttg
     6721 accatctccg ggtcaagcgc cgaagtcggc tcgtcgaata acatggcaac cggatccatc
     6781 gccagcgcgc gcgctatggc gacccgctgc tgctgacctc cggagagctg accaggaaat
     6841 ttatcggtct gatcgcttaa accgacacgt tcaagcaggg tacgcccacg ttcaagcgct
     6901 tctttttcgg gacgccccaa aaccttgact tgcgccagcg ccaggttgcg catcaccgac
     6961 aagtgtggaa atagctcgaa gtgctggaat accatgccta tgcgcgaccg cagtttcggc
     7021 aagttcgttt cgggagcgcc gacggagatg ccgtcgatcc agatttcccc cttctgaaac
     7081 ggctcgagcg catttgccgt tttaatcagt gtcgacttgc ccgaacccga cggcccgcac
     7141 accacgacga cttcgcccgt tgcaaccgac gtggagcaat ctttcaatac ctggaacgag
     7201 ccataccact tgctcacatt tttaaattca atcactaaaa accccttgga ggtcagcgga
     7261 caatagccat acgttgttca agaattctaa ctgtgcgaga cgcgccataa gaaataagaa
     7321 aataaatgac agcagcgaag ataaacattt caaccaggcg cccgtcgcgc tgggcgacgt
     7381 tattggcggc gcccaaaaag tcggtcaggg acagcacata aaccagcgag gtgtcctgaa
     7441 acaaaacaat catctgcgtc agcagcaccg gcgtcatgtt gcgaaacgct tgcggtaaaa
     7501 caatgtggcc catgacttgc cagtaattca ggcccaaagc atagccggcg
//
LOCUS       DATCXU010000487         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_912937,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000487 DATCXU010000000
VERSION     DATCXU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912937"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VIR04_10870"
     CDS             <1..1153
                     /locus_tag="VIR04_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY9461288.1"
                     /translation="AGYFGYDTVRHIEPRLGAATKPFPEGQESGTPDIMLLQIDELVI
                     VDNLAGRTYLVVYADPQQSESYARAKRRLRELREKLRTPVVIPYAYASMQTASQRNFK
                     KEDYIAAVLKAKEYIAAGDVMQVQIGQVISKPFRDSPLSLYRSLRSLNPSPYMYYWDF
                     DEFQVVGSSPETLVRQEIVTEGDRKKSVITIRPLAGTRKRGATPEQDAELAKELLADP
                     KERAEHVMLIDLARNDVGRVAEVGSVKVTDTMNIERYSHVMHLVSNVTGVLHQGMSNM
                     DVLRAAFPAGTLTGAPKVRAMEIIDELEPVRRGIYGGAAGYLSYGGKMDVAIAIRTGI
                     VKDGMLYVQAAAGIVADSDPEKEWLETEAKARAVLRAAEQVQFGLDEPI"
     gene            1185..1748
                     /locus_tag="VIR04_10875"
     CDS             1185..1748
                     /locus_tag="VIR04_10875"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY9461289.1"
                     /translation="MLLMLDNYDSFTYNLVQYFGELGEEVKVVRNDQATVAEIAAMNP
                     ARICVSPGPCSPAEAGISIALIQYFGGKVPILGVCLGHQAIGAAYGGKVVRAHQLMHG
                     KTSPVSHSGTDVFKGLPSPYTVTRYHSLAIERESLPDCLEITAETQDKEIMGVRHKTL
                     PVYGVQFHPESILTEHGHTLLRNFLSL"
     gene            1763..2818
                     /gene="trpD"
                     /locus_tag="VIR04_10880"
     CDS             1763..2818
                     /gene="trpD"
                     /locus_tag="VIR04_10880"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY9461290.1"
                     /translation="MTNKNTSGAVITPTEALVRCIEHREIFHDEMLHLTRMLMRGEMS
                     PQIASALLMGLRVKKETIGEITAAAQVMREFALPVPTAHPEELLDMCGTGGDGSHTFN
                     ISTAAMFVVAAAGVKIAKHGNRSASSSSGSADVLESLGANLALSPEQVAQSIEQTGIG
                     FMFAPAHHGAMKNVAAVRKELGVRTIFNILGPLTNPASAGNQLMGVFHADLVGIQVRV
                     LQRLGSKHVLVVHGKDGMDEASLGAATLVGELKNDTVREYEIHPEDFNMAMVSNRSLR
                     VNNRDESAKLVIQALENVKGAARDIVGFNAGLAIYAGNKVDSIQDGLTLAFETIASGA
                     GRAKLEEFCSFTRSLRA"
     gene            2815..3603
                     /gene="trpC"
                     /locus_tag="VIR04_10885"
     CDS             2815..3603
                     /gene="trpC"
                     /locus_tag="VIR04_10885"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY9461291.1"
                     /translation="MNSILAKILENKKAEVATARQMRSEADVLREAKNRKDLRGFVQA
                     IEDKISQGKPAVIAELKKASPSKGLIRESFNVVEIANSYAAYGAACLSVLTDVKFFQG
                     SYDYLRQARAACALPVLRKDFMIDPYQIVHARAIGADCILLIVAALTPQQLREFEDVA
                     TELGMDVLVEVHDRAELDVALELRTPLIGINNRNLRTFKTSLKTTIDLLPVIPPTKRV
                     VTESGILTRDDVDLLREHKVEAFLVGEIFMRAEEPGIMLRDLFF"
     gene            3610..4407
                     /locus_tag="VIR04_10890"
     CDS             3610..4407
                     /locus_tag="VIR04_10890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY9461292.1"
                     /translation="MASPKPAATDIAHNVLQNDLLTYVQRLDPAWQDALKGPATQAAL
                     HRLHCFLADRRADGAEIYPGDPFRALNMVPPDAVQVIILGQDPYHGPNQAQGLAFSVP
                     DICRCPPSLRNIFKELALEYPGQAQPQRHDLTRWARQGVLLLNAVLTVEAHQPASHMR
                     KGWELITDAIIQHAARAPQPKVFMLWGNYAQAKQALLPDTSTGLRQVLIANHPSPLSA
                     QRPPRPFIGCQHFTQANAWLRQHGQTGINWLDNAVVASQQHEVYAHS"
     gene            4677..>5917
                     /locus_tag="VIR04_10895"
     CDS             4677..>5917
                     /locus_tag="VIR04_10895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY9461293.1"
                     /translation="MSFEALGLAPALLSAVEKAGFTKPTNVQEAAIPKALAGLDLMVS
                     SQTGSGKTAAFMLPALNRIASTPNKGSGVQVLVLTPTRELAMQITDATRVYGAHLKDL
                     RTATVVGGMPYAAQLKALSRRVDVLVATPGRLIDHLNSRRVNLSTVHTLVLDEADRML
                     DMGFIEDIETIVSRTPDDRQTLLFSATLDHTITRLASKMMRDPLQIEVSGHKQPNTSI
                     TQTLLYADDNGHKMRLLDHLLRDTTLDQAIIFTATKRGADDLADKLSERGFSAAALHG
                     DMNQRQRTRTLGLLQRHQLQILVATDVASRGIDVQSISHAINYDLPMQPEDYTHRIGR
                     TGRAGHSGLAFTLAVHSERGKIRRIEQFIGRPIPIHVVEGLEPKKTARPSFSDRAGNG
                     GKRFGGNSRSFGVNGRGGFGGG"
BASE COUNT         1319 a         1762 c         1651 g         1185 t
ORIGIN      
        1 ggccggctac ttcggctacg acacggtgcg ccacattgaa ccgcgtctgg gcgccgccac
       61 caagcccttc ccggaaggcc aggaatccgg cacgcccgac atcatgctgt tgcagatcga
      121 cgaactggtg atcgtcgaca acctggccgg ccgcacatac ctggtggtgt acgccgaccc
      181 gcagcaatcg gaaagttatg cgcgcgcgaa gcgccgtctg cgcgaattgc gcgaaaagct
      241 gcgtacgccc gtggtcatac cctacgccta cgccagcatg caaaccgcca gccagcgcaa
      301 tttcaagaaa gaagactaca ttgccgctgt attgaaggcc aaggaataca ttgccgccgg
      361 cgatgtgatg caagttcaga tcggccaggt catcagcaaa cccttccgcg attcaccgtt
      421 gtcgctttac cggtcgttgc gttcattaaa cccatcgccg tatatgtact attgggattt
      481 cgacgaattt caagtggtgg gttcctcgcc cgaaaccctg gtgcgccagg aaatcgtgac
      541 agaaggcgac cgcaaaaagt cggtcataac cattcgccct ctggccggaa cccgcaagcg
      601 gggcgccact cccgagcagg atgccgaact ggccaaagag ttgctggccg atcccaaaga
      661 acgcgccgaa cacgtcatgc tgatcgacct ggcgcgcaac gacgtggggc gcgtggcgga
      721 agtcggatcg gtcaaggtca ccgacaccat gaacatcgag cgctattctc atgtcatgca
      781 tctggtctcg aacgttaccg gcgtactgca tcagggcatg agcaatatgg acgtcctgcg
      841 cgcggctttc ccggcgggca cgctgacggg ggcgccgaag gtgcgcgcca tggaaatcat
      901 cgacgagctt gagcctgtgc ggcgcggcat ttacggcggg gcggcgggct accttagtta
      961 cggcggcaaa atggacgtcg ccatcgcgat tcgcaccggc atcgtcaaag acggcatgct
     1021 gtatgttcag gcggcggccg gaattgtggc cgattcggat cctgaaaagg aatggcttga
     1081 aactgaagcc aaagcgcgcg cggtgctgcg cgccgccgaa caagtgcagt tcggtctgga
     1141 cgagcccatt taaaccatta agcattgcct caaaggagca taaaatgcta ttaatgcttg
     1201 ataactatga ttcttttacc tacaacctgg ttcagtattt tggtgaactg ggcgaagaag
     1261 tgaaggtggt gcgcaatgac caggccactg tggccgagat tgcggccatg aatccagccc
     1321 gaatttgcgt ctcgcccgga ccttgctcac ccgccgaagc gggtatatcc atcgcgctta
     1381 ttcagtactt tggcggtaaa gtgccgatcc tgggtgtatg cctgggccat caggccatag
     1441 gcgcggccta cggcggaaaa gtggtgcgcg cccatcaact catgcatggc aagacctcgc
     1501 cggtcagcca ttcgggcacg gacgttttca aggggctgcc ctcgccctat actgtgaccc
     1561 gctaccattc cctggcaatc gagcgggaat cgctgccgga ttgccttgaa atcactgccg
     1621 aaacccagga caaggaaatc atgggcgtac gtcataaaac tctgccggtc tacggcgttc
     1681 agttccatcc cgagtcgatt cttacggaac atggccacac cttattacgc aatttccttt
     1741 cgctttaacg gacgatcctt caatgacgaa caaaaatacc tccggcgcag tcatcacccc
     1801 cactgaagcc ctggtgcgct gcatcgaaca ccgcgaaatc ttccacgatg aaatgctgca
     1861 cctgacgcgc atgctgatgc gcggcgaaat gtcgccgcaa atcgccagcg ccctgcttat
     1921 gggtttgcgg gtcaaaaagg aaaccattgg ggaaattacg gcggcggcgc aggtcatgcg
     1981 cgaattcgca cttccggttc ctacggccca ccccgaagaa ctgctggata tgtgcggcac
     2041 gggcggcgat ggatcccaca cattcaatat ctccacagcg gccatgttcg tggtcgccgc
     2101 cgccggggta aaaatagcca aacatggcaa ccgaagcgcg tcgtcatcgt cgggcagcgc
     2161 cgatgttctg gaatcgctgg gcgccaattt ggcgctaagc ccggaacaag tggcgcaaag
     2221 cattgaacag acaggaatag gctttatgtt cgccccggcc caccatggcg ccatgaaaaa
     2281 cgtggcggct gttcgtaaag agctgggcgt gcgcacaata ttcaacattc ttgggccgct
     2341 caccaacccg gccagcgcgg gaaaccagct tatgggcgta tttcatgccg acctcgtcgg
     2401 catccaggtt cgcgttctgc agcgtttggg ttcgaagcac gttcttgtgg tgcacggcaa
     2461 ggacggtatg gacgaagctt cgctgggcgc ggccacactg gttggcgaac tgaaaaacga
     2521 tacggtgcgt gaatacgaaa tccatcccga ggacttcaat atggccatgg tgtcgaaccg
     2581 gtcgctcagg gtaaacaacc gggacgagtc ggctaaatta gtcattcagg ctcttgaaaa
     2641 cgtgaaaggc gccgcccgcg atatcgtcgg gttcaacgcc gggctggcca tttatgccgg
     2701 caacaaggtc gattcgatcc aggacggatt gacactagcc tttgagacga ttgccagcgg
     2761 cgcaggcagg gctaaactgg aagagttttg ttcctttaca cggtcactgc gcgcatgaat
     2821 agtatccttg ccaaaatcct cgaaaataaa aaggccgagg tcgcaaccgc gcgtcaaatg
     2881 cgcagcgagg ccgatgtgct gcgcgaggcg aaaaaccgca aggacctgcg cggcttcgtt
     2941 caggccattg aagacaaaat atcgcagggc aagccggccg tcatcgccga actgaaaaaa
     3001 gcctcgcctt cgaaaggact tatacgcgaa agcttcaacg tggtggaaat cgccaactcg
     3061 tacgccgcct acggagccgc ctgcctttcg gtgctgaccg atgtaaagtt ttttcaaggc
     3121 tcgtacgatt atttgcgcca ggcgcgtgcc gcctgcgctc tgccggtgct gcgcaaggac
     3181 ttcatgatcg atccctatca aattgtgcat gcgcgcgcaa taggggcgga ctgcatattg
     3241 ctgattgtcg cggcgcttac gccgcaacag ttgcgcgaat ttgaagacgt ggccaccgag
     3301 ctgggcatgg acgttctggt cgaagttcat gaccgggccg agctggacgt tgcgctggaa
     3361 ttgcgcacac cgctcatcgg catcaacaat cgcaatctgc gcacattcaa aacttcgctt
     3421 aaaaccacga ttgatctcct tccagtcatt ccaccgacca agcgtgtggt caccgaaagc
     3481 ggcattctga ccagagatga cgtcgacctt ttacgcgaac acaaggtcga ggcgtttctg
     3541 gtgggcgaga tattcatgcg cgccgaagag cccggaatca tgctgcgcga tttattcttc
     3601 tgattttcca tggcatcgcc caaacccgcc gctacagaca tcgcgcacaa cgtattgcaa
     3661 aacgatttgc tgacctatgt tcaacgtctt gatccggcat ggcaagacgc attaaagggt
     3721 ccggcaaccc aggcggcgct gcatcgcctg cactgctttt tggctgatcg gcgggcggat
     3781 ggcgccgaaa tttatcccgg cgatccgttt cgggcgctta acatggtgcc gcccgacgcg
     3841 gtgcaagtga tcattctggg gcaggatccc tatcacggac ccaaccaggc gcaaggcctg
     3901 gcgttttccg tgccggacat ttgccgttgt ccgcccagtc tgcgcaatat tttcaaggaa
     3961 ctggcgctgg aatatccggg gcaagcgcaa ccgcaacgcc atgacctcac acgttgggcc
     4021 aggcaagggg tgttgttgct gaacgcggtt ttgacggttg aagcgcacca acccgcttcc
     4081 cacatgagaa aaggctggga actgataacc gatgcgatta ttcagcatgc ggcgcgcgcg
     4141 ccgcaaccga aagtattcat gttgtggggc aattacgcgc aggcaaagca ggctctgctg
     4201 cccgacacgt ctaccggcct tcggcaggtg cttattgcca accacccctc gccgctatcg
     4261 gctcagcgcc cgcccaggcc cttcataggc tgccagcatt tcacgcaagc caatgcctgg
     4321 ctgcggcagc acggccagac aggaatcaac tggctcgata acgcggttgt tgcatcgcaa
     4381 caacacgagg tttacgcgca tagttgaaac ggactatact gcggccactg cattttcata
     4441 gcggcgacat tctttcacag cactattgtc tgtgtcatag cagttgactg gttttccagc
     4501 acccatgtcg tacgccatcg ctccctgacc cccgagcctt tccgttgcct gttacctcgg
     4561 tacacgctgc attttaggtt gattggtcgg cacgatgctc caaccaacct gcgcaaaagg
     4621 cgttactttt gcctgcgcgc tcgtgtatgc gccgtttaac cggcaaagga tcacgcatgt
     4681 ctttcgaagc actgggtctt gcacccgccc ttttatccgc cgtcgagaag gcgggtttca
     4741 ccaaacccac gaacgtacaa gaagccgcca tacccaaggc attagcaggc ctggacctga
     4801 tggtctcttc tcaaacaggc agcggcaaaa cggccgcctt catgctgccc gcattgaatc
     4861 gtattgccag cacgcccaac aagggctcag gcgtacaggt gctggtgctg acccccacgc
     4921 gcgaactggc catgcaaata accgacgcaa cccgcgtata tggcgcgcat ctgaaagacc
     4981 tgcgcacggc aacggtcgtc ggcggcatgc cttatgccgc ccaactgaag gcgttgtcgc
     5041 gccgcgttga tgtgctggtc gcaaccccgg ggcgcctgat cgatcacctg aattccagac
     5101 gcgttaattt gtccacggtt cacaccctgg ttctggacga agccgatcgc atgctggaca
     5161 tgggctttat cgaagatatt gaaacaatcg tcagccgcac gcccgatgat cgccagacct
     5221 tgctgttttc agcaacgctg gaccacacga ttacccgcct ggccagcaaa atgatgcgcg
     5281 acccattgca aatcgaggta tcgggccaca agcagcccaa caccagtatt acgcaaacac
     5341 tgctgtatgc cgacgataac ggtcacaaaa tgcgcttgct cgaccattta ttgcgcgata
     5401 cgacgctgga ccaggcgatt atcttcaccg ccaccaaacg cggcgccgat gatctggccg
     5461 acaagctttc agagcgcggc ttttcagccg ccgcgctgca tggcgacatg aatcagcgcc
     5521 aacgcacacg cacgcttggt ctgttgcaac ggcatcaatt gcaaattctg gtcgcgaccg
     5581 acgtcgcttc gcgcgggatc gatgtgcaaa gcattagcca cgccatcaac tacgacctgc
     5641 cgatgcagcc cgaagattac acgcaccgta tcggccgcac aggccgcgcg ggtcacagcg
     5701 gcctggcgtt caccctggcc gtacattccg aacgcggcaa aattcgccgc atcgaacagt
     5761 ttatcggccg gcctattccc attcacgtcg tcgaagggct ggaacccaaa aagacagcac
     5821 ggcccagctt ctccgaccgt gccggcaatg gcggcaagcg gtttggcgga aatagtcgca
     5881 gctttggcgt caatggccgt ggcggttttg gcggcgg
//
LOCUS       DATCXU010000488         5480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_527265,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000488 DATCXU010000000
VERSION     DATCXU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527265"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VIR04_10900"
     CDS             complement(<1..553)
                     /locus_tag="VIR04_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY9461294.1"
                     /translation="MAVCTRCGYVLYRQSAVPLGGWIALTLGALLVFLIANYFPIATL
                     ALKGATVNVSFPDALALTWRQGHASLAIMTGLFGFVLPVMQLLFLLWALLAISSRRLP
                     ADFRYGMRLLDAMAPWSMVPVLMLGILVPMVKLAGLASLIIGPGLWAFGTLTFLLTGL
                     SRLSAQRLWRHAEDEHLVMQSGDR"
     gene            complement(628..2946)
                     /gene="clpA"
                     /locus_tag="VIR04_10905"
     CDS             complement(628..2946)
                     /gene="clpA"
                     /locus_tag="VIR04_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY9461295.1"
                     /translation="MISEELEVSLHMAFVEARTARHEFITVEHLLLALLDNVAAIDVL
                     RACSANIDALRHELRKFIAENTPVFPGEDEVDTQPTLGFQRVVQRAIMHVSSGNSNKN
                     TVTGANVLVAMYGEKDSHAVYFLLQQGVTRLDVVNFLSHGITKTAEDVGSGPVKALGV
                     EHEQKSDQQKSALELYALDLNFEAREGRIDPLIGREHEVERVIQVLCRRRKNNPLLVG
                     EAGVGKTAIAEGLAWRITKGDVPEILADSEVYALDMGALLAGTKYRGDFEQRLKAVLK
                     SLKENTEAILFIDEIHTLIGAGSASGGTLDASNLLKPALSSGQLKCMGATTYNEYRGI
                     FEKDHALSRRFQKIDVAEPTVAQTIQILRGLKARFEAHHGVRYSAAAIVAAAELSARH
                     INDRYLPDKAIDVIDEAGAAQRLLPRSKQKKLIGKAEVESTVAKIARIPAQSVSSNDR
                     NKLATLERDLKTVVFGQDAAIQALAGAIKMARSGLGRPDKPIGSFLFSGPTGVGKTEV
                     ARQLAFTLGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAIGKQPHCVLL
                     LDEIEKAHPDVYNILLQVMDHGTLTDNNGRKADFRNVIIVMTTNAGAEALNHKSIGFA
                     ASATAGDEMVDIKRMFTPEFRNRLDAIIGFASLSREVILRVVDKFLMELEDQLHEKRV
                     EVVFSQALRDYLAKKGFDPSMGARPMQRLIQDTIRRALADELLFGRLVEGGHVVVDLD
                     SADKVVLTFTAKADKSAKSKHPPVDSEAELVD"
     gene            complement(3163..3486)
                     /gene="clpS"
                     /locus_tag="VIR04_10910"
     CDS             complement(3163..3486)
                     /gene="clpS"
                     /locus_tag="VIR04_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY9461296.1"
                     /translation="MATFIENQRDTVLDRQSSKLAPPPMYQVVLLNDDYTPMEFVVGV
                     LQRIFGKDEDEAARIMLKVHHEGRGVCGVYSRDIAATRVEMVRQLARARQHPLQCVME
                     PAPDA"
     gene            3679..3924
                     /locus_tag="VIR04_10915"
     CDS             3679..3924
                     /locus_tag="VIR04_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY9461297.1"
                     /translation="MSDSTATSAPGETQKLTGTVKWFNDAKGFGFITPDNGGEDLFAH
                     FSSIQMNGFKTLKEGQKVAFEIAQGPKGKQALNITAA"
     gene            3951..4439
                     /locus_tag="VIR04_10920"
     CDS             3951..4439
                     /locus_tag="VIR04_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY9461298.1"
                     /translation="MMVKQNSTTGLLGSVFFCLAMLTIASTAHGKTTAQQDAPQAMLL
                     PTTELSIGQQSVHAEVAATEASRQRGLMYRQLLPNNQGMLFVFDEAAVQCFWMKNPPL
                     PLSIAFIDARGMIVNITDMQAQSTDVHCPAGPIRYALEMKQGWFTEHHIGAGLPVAGL
                     PR"
     gene            complement(4501..5079)
                     /gene="sodB"
                     /locus_tag="VIR04_10925"
     CDS             complement(4501..5079)
                     /gene="sodB"
                     /locus_tag="VIR04_10925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEY9461299.1"
                     /translation="MAYTLPPLPYALDALAPHISKETLEYHYGKHHQSYVANLNKLIA
                     GTEFESASLEDIVKRSSDGLFNNAAQTWNHTFYWNSLSPNGGGEPKGALLTAINAKWG
                     SVQAFKEAFSKAAAGNFGSGWTWLVKKPGGKLDIVNTSNAATPITTPDQPLLTCDVWE
                     HAYYIDYRNARPKYLENYWDLVNWDFGAKNLG"
     gene            complement(5131..>5480)
                     /locus_tag="VIR04_10930"
     CDS             complement(5131..>5480)
                     /locus_tag="VIR04_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY9461300.1"
                     /translation="ALVPGERRRSRLEFVKSRLTRCAPDHAYLERDLARCTQRLASLG
                     QRQVTSRRDRLAAVSQTLAALNPRNVLGRGYAIVRDGQGAIIKNALDLSIGDQLGIEL
                     HQGRANVSVLSTT"
BASE COUNT         1301 a         1510 c         1479 g         1190 t
ORIGIN      
        1 ggcggtcgcc cgactgcata accaagtgct catcctcggc atggcgccat aaacgttgcg
       61 cggaaagccg gcttaaaccg gtgagcagga atgtcagcgt tccgaacgcc cacaggcccg
      121 gcccgattat aagcgacgcc aggccggcca gtttgaccat cggaaccagt attccaagca
      181 ttaaaaccgg aaccatgctc cacggcgcca tggcgtcgag caggcgcatg ccataacgaa
      241 aatcagcagg caagcgcctg gacgaaatgg ccagcagcgc ccataacaaa aagagaagct
      301 gcataacggg caaaacaaag ccgaacagcc cggtcattat cgcaagggag gcatggcctt
      361 gccgccaagt gagcgcaagc gcgtcgggaa acgatacgtt tacggtcgcg cccttgaggg
      421 caagggtcgc aattggaaaa taattggcaa tcagaaaaac aagtaaggcg cccagtgtca
      481 gggcaatcca gccgcccaat ggaaccgcgc tttgtcgata caggacatag ccgcaacgag
      541 tgcagaccgc cattgcccca gcggcgatgg gtgtgcgcct gtgcaggcga tcacaatgcg
      601 gacaggcaat tgcaggatgc cccgccatta atcgacgagc tcggcttccg agtcaacagg
      661 aggatgtttc gacttggccg acttatccgc cttggcagtg aaagtgagga cgaccttgtc
      721 ggcactgtcg aggtccacaa cgacatgccc gccttcgacc aggcggccaa acagaagttc
      781 gtcggccagc gctcggcgta tggtgtcctg gataagccgt tgcatgggcc gggcgcccat
      841 ggaaggatcg aagccttttt tggccaggta atcgcgcaac gcctgggaga acacgacctc
      901 gacccgcttt tcatgcagtt gatcttcgag ttccatcagg aatttgtcca ccacccgcaa
      961 aatgacttcg cgcgacagcg aagcgaaacc gatgatcgca tcgagccgat ttcggaattc
     1021 gggcgtgaac atgcgcttga tgtcgaccat ttcgtcgccc gcagtggcag aggccgcgaa
     1081 accgatggat ttgtggttaa gagcttcggc gcccgcattg gtggtcatga caatgattac
     1141 attgcgaaaa tcggctttgc gcccgttgtt gtccgtgagc gtgccgtggt ccatgacctg
     1201 caacaatata ttgtaaacat cggggtgggc tttctcgatt tcgtcgagca gcaaaacgca
     1261 gtgcggttgc ttgccgatgg cctccgtgag cagacctccc tggtcgaaac cgacatatcc
     1321 gggcggcgcg ccaatcagtc gcgacaccgt gtgacgctcc atgtattcgg acatatcgaa
     1381 acgcagcagt tcaacaccca gtgtgaatgc caattgacgc gcgacttcag tttttcctac
     1441 gccagtgggg ccggaaaata aaaacgaccc aatcggtttg tcgggccggc ccaagccgga
     1501 gcgcgccatc ttgatggcgc cagccagcgc ctgaatggcc gcatcttggc cgaacaccac
     1561 tgttttaagg tcgcgttcga gcgtggccag cttattacga tcgttgctgg atacactttg
     1621 cgccggaata cgggctattt ttgcaaccgt agattcgacc tcggctttgc ctatcagctt
     1681 cttttgcttg gaacgcggca gcaagcgctg ggctgcgccg gcttcgtcga tgacgtcgat
     1741 agccttgtcc ggcaaatagc gatcgttgat atggcgagcc gacaattcgg ccgccgcaac
     1801 aatggccgcc gccgaatacc gtaccccatg gtgcgcctcg aagcgggcct tcaggccgcg
     1861 caagatctga atggtttgcg caaccgtcgg ctcggcgacg tcgatttttt gaaaacggcg
     1921 cgacagagca tgatcttttt caaatatgcc gcgatattcg ttataggtgg tcgcgcccat
     1981 gcacttcagt tggccggacg ataacgcggg tttgagcaga ttggaggcgt cgagagtccc
     2041 gcccgaggcc gatcccgccc cgatcaacgt gtgaatttcg tcgatgaaca aaatggcttc
     2101 ggtgttttct ttcagcgact tgagcaccgc cttcaggcgc tgttcaaagt cgccccggta
     2161 cttcgtgccg gccagcaaag cgcccatgtc caacgcatag acttcggagt cggccagaat
     2221 ctcgggaacg tcgcctttgg tgatgcgcca tgcaagaccc tcggcgatcg cggttttccc
     2281 aacgccggct tcgcccacca gcagagggtt gtttttacgg cgccggcaca aaacctgtat
     2341 gacgcgttcg acctcgtgtt cgcgcccgat taaaggatcg attcgacctt cccgcgcttc
     2401 gaagttcaaa tccaacgcgt agagttcgag cgcggatttt tgctgatcgg atttttgctc
     2461 gtgttcgacg cctagcgcct taaccggccc ggagccgacg tcttcggccg ttttggtaat
     2521 accgtgcgac aagaaattaa ccacatcaag gcgggtgacg ccctgttgca gcagaaaata
     2581 cacggcatgc gaatcttttt cgccatacat ggcgaccaaa acattggccc ccgtaacggt
     2641 attcttgttg gagttgccgg aggaaacatg catgattgcg cgttgaacga cgcgctgaaa
     2701 gcccaatgtg ggctgggtgt cgacttcgtc ttcgccgggg aaaaccggtg tgttttcagc
     2761 aatgaacttg cgcagttcgt gacgcaacgc atcgatattg gcggaacacg cgcgcaacac
     2821 atctatcgcc gcgacattgt ccagcaacgc gagcaataag tgctcgacgg taataaattc
     2881 atgtctggct gtgcgggcct cgacaaaggc catgtgcaag ctaacttcaa gttcttcgga
     2941 aatcacgctt cctccattgc atatcgaagc ggacaaacaa attcggttaa ttctattcta
     3001 tgactaattc ggtgctgagt aatcaaccat tcatcgcata gggacaatac tgtgaccgga
     3061 taacatcaat aatacatgcc gccgattacc aaagccagag aaaaagcagg ctgtaaatac
     3121 cctaaaccgt accatactcg ccagtgattg agaactgcgc catcaggcgt cgggagcagg
     3181 ctccattacg cactgcaacg ggtgctggcg cgcgcgcgcc aactggcgaa ccatttccac
     3241 gcgagtcgcg gcgatgtcgc gcgaatacac accgcaaacg ccgcgtccct cgtgatgaac
     3301 cttcagcata atacgtgccg cttcgtcctc atccttgcca aaaatccgct gcaatacgcc
     3361 caccacaaac tccatgggcg tgtagtcatc gttcagcaag accacctgat acatcggcgg
     3421 cggcgccagc ttgctgctct ggcgatcgag caccgtatcg cgctgatttt ctataaaagt
     3481 agccataagt taaaaaatca aatactagtg ctttccatac ttgactacga tggtgtaaat
     3541 tacacattat catagaattg tgagtgtttt cacgtatttg atagtggtac cagaatgtat
     3601 agactaaaag ttagggcttg tagccgtatg cagcctgtat tcagttttaa ttaacactat
     3661 agtcgagagg cagtccgtat gtcagattca accgcaacct ccgcgccagg ggagactcaa
     3721 aagctgacgg gaaccgtaaa atggttcaat gatgctaaag gtttcggctt tattacccca
     3781 gacaatgggg gcgaagatct tttcgcccat ttttcgtcca ttcaaatgaa cggattcaaa
     3841 acgcttaaag agggccagaa agtggcgttc gaaatagcac agggtcccaa gggtaaacaa
     3901 gctttaaata tcacggctgc ctgatcgaac gcaagcaacc cattccaaga atgatggtta
     3961 aacaaaactc aacaacgggg ctgcttggct ccgttttttt ttgccttgcc atgctgacta
     4021 ttgcaagcac ggcgcacggc aaaactacgg cgcaacaaga cgcgccccag gccatgctgc
     4081 taccgaccac cgagctttct atcggtcaac aatccgtcca tgcggaagtt gccgccaccg
     4141 aggccagccg gcaacgcgga ttaatgtatc gacaattgct gccgaacaat cagggcatgt
     4201 tgtttgtgtt cgacgaagcg gccgtccaat gtttctggat gaaaaacccc ccgctgccgc
     4261 tgtccatcgc attcattgat gcccgtggca tgatcgtaaa catcaccgat atgcaggcgc
     4321 aaagcactga tgtgcactgc ccggcgggtc caattcggta cgcgcttgaa atgaaacaag
     4381 ggtggtttac agagcaccac attggagcgg gtctgccggt tgcaggcctg ccgcgctaac
     4441 aacttgcgca aaaacgacaa aagccccttg gggcttttgt cacggctgaa ttcgcccgga
     4501 ttacccgagg ttctttgcgc cgaaatccca attgaccaga tcccagtaat tttcaagata
     4561 cttgggacgt gcattgcgat agtcgatgta ataagcatgt tcccagacat cacaggtcag
     4621 caaaggctga tccggcgtcg taatgggcgt agcggcattg cttgtgttga caatatcgag
     4681 cttgccaccg ggctttttaa ccagccaggt ccagcccgaa ccgaaattgc cggccgccgc
     4741 tttgctgaaa gcttctttaa aggcctggac actgccccat ttggcattaa ttgccgtgag
     4801 caaggcgccc ttgggttcgc cgccgccatt cggagacagg ctattccagt aaaaagtgtg
     4861 attccaggtt tgagctgcgt tgttgaacag gccgtccgat gaccttttta cgatgtcttc
     4921 aagcgaggcc gattcgaatt ctgtgccagc aattaattta ttaagattgg caacataaga
     4981 ctggtgatgt ttgccatagt gatattcaag agtctctttc gaaatgtggg gcgcaagcgc
     5041 gtccagagca tacggaagtg ggggaagcgt ataagccatg ttcaatttcc ttcaaaaagt
     5101 tattcgcaaa tgaaatgcgg gcgtctgtag tcaagtcgtt gacaaaaccg acacgttcgc
     5161 ccgtccctgg tgcagctcaa tacccagttg atcgccaatg ctcaagtcta acgcgttttt
     5221 gattattgct ccctggccgt cgcgcacaat tgcatacccg cgtcccagaa catttcgggg
     5281 attgagcgcg gcaagcgttt gagagacggc cgccaagcgg tcgcggcggg acgtgacctg
     5341 gcgctgaccc aggctggcca ggcgttgcgt gcagcgcgcc agatcgcgtt ccaagtatgc
     5401 atgatcgggc gcgcaccggg tcagccggct tttgacgaat tccaaacggg agcggcggcg
     5461 ttctccggga accagcgccg
//
LOCUS       DATCXU010000489         9947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_732595,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000489 DATCXU010000000
VERSION     DATCXU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732595"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VIR04_10935"
     CDS             <1..885
                     /locus_tag="VIR04_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY9461301.1"
                     /translation="NILGFARAVHERYPGTPIAFCAILPYDRRLDLEKLGCITLEDPS
                     VAVRTISAILKRNRNARPKAIAVQSINLPAHTPALNEAESLLALKQAHVPVVEFKLAS
                     SKEEAASLVEQLNAPAAMKILSADIAHKSDIGGVKLSVRGPDEARAAYDAIMSSVARH
                     APLAGLDGMLIAPMAPDGVECIIGMHRDPTFGPVIMFGLGGIFVEILKDVSFRLAPFD
                     KAEALEMIQQTRAVSLLKGARGRAPSDLDALAESLAALSNMAYAARDRISSIDINPFL
                     VLPAGQGAMALDALILQD"
     gene            986..1765
                     /locus_tag="VIR04_10940"
     CDS             986..1765
                     /locus_tag="VIR04_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY9461302.1"
                     /translation="MSEASTIKNKIASAAQLLLNARSTQKLLPELPSEYRPGNSAEAY
                     AIQDAVVAQLGPIGGWKVGAGSATAEPNCSPLPGSLIFNGSGSFSSRVYTLRGIEGEI
                     GVRMKRDLPPRAEPYSIPDVISAVGSVHPMIEVVESRFTDRGKVDPLSALADAASNGA
                     LVYGEGRTDNLAIDQASQLFELYFDSKKVVELVGGNSAGNIWRLMAWLANHVAARCGG
                     LRAGQIVTTGSCTGVTYAKPGARVKVSSPGLGKAELSFSVL"
     gene            1762..2613
                     /locus_tag="VIR04_10945"
     CDS             1762..2613
                     /locus_tag="VIR04_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEY9461303.1"
                     /translation="MKVPKRLAPLLDEGLIDEVLGQLMSGKEATVYIVRSGNETRCAK
                     VYKDVKQRSFRQATSYRDGRSVKNSRQARAMEKGTRYGREVQEQLWQTAEVDALFRLT
                     NAGVRVPQPYICTDGVLLMELVLDAAGDVAPRLNDVDLTQEQALDLHARLLNQVVRML
                     CAGVIHGDLSEYNILLAADGPVIIDLPQAVDAAANNQAKAMLERDVDNLATYFGRFAP
                     QLLVANYGKEIWALFEAGNLSADMELTGRVELDTRPVDLDGLMDDIEDARFEEDARRR
                     RAAELRS"
     gene            2615..3316
                     /locus_tag="VIR04_10950"
     CDS             2615..3316
                     /locus_tag="VIR04_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY9461304.1"
                     /translation="MKIVMVLTSHDRLGDTGQKTGFWLEEFAAPYYVFKDAGVDITLA
                     SPSGGQPPVDPKSDAADSQTDATRRFKADPAAQAALAHTRKLSDVLATDFDAVFYPGG
                     HGPLWDLAENASSIALIEATFASGKPVVAVCHAPGVLRHAKAPDGTPLVRGKTVTGFT
                     NTEEEAVGLTHVVPFLVEDALKKNGGHYSKAGDWQSHVVSDGNLITGQNPASSVAAAK
                     ALLDLLGHANQGIAR"
     gene            3403..5088
                     /gene="ilvD"
                     /locus_tag="VIR04_10955"
     CDS             3403..5088
                     /gene="ilvD"
                     /locus_tag="VIR04_10955"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY9461305.1"
                     /translation="MSTAKHLRSAHITEGVERAPNRSMYYAMGYTEEDFKKPMVGVAN
                     GHSTITPCNSGLQKLADAAIEAIEEAGGNAQVFGTPTISDGMAMGTEGMKYSLISREV
                     IADCVETCVQGQWMDGVLVIGGCDKNMPGGMMGMLRANAPAIYVYGGTILPGCYQGKD
                     LNIVSVFEAVGEHAAGRLSTTDLRQIELHAIPGCGSCGGMYTANTMSSAFEAMGMSLP
                     YSSTMANEHAEKVESAKESARVLLRAIELDLKPRDIVTRESIENAVSVIMATGGSTNS
                     VLHFLAIAHAAGVQWTIDDFERVRQRVPVFCDLKPSGEYLVVDFHRAGGIPLVMKMLL
                     NAGLLHGECMTITGKTIAETLKDVPDTPAANQHVIHTIDKPLYPQGHLAILKGNLSPE
                     GSVAKITGLKNPVMTGPARVFEDEQSGLKAILDGKIKAGDVMVLRYLGPKGGPGMPEM
                     LAPTGALVGAGLGDSVGLITDGRFSGGTWGMVVGHVAPEAAVGGCIALVQEGDSITID
                     AHHQLLQLNVPDDEIAARRAKWVAPKPRYLRGVQAKFAFNVSTASLGAVLDSF"
     gene            complement(5109..6407)
                     /locus_tag="VIR04_10960"
     CDS             complement(5109..6407)
                     /locus_tag="VIR04_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9461306.1"
                     /translation="MTAANKTSAELVDSPYAIARLLLTLTLMTIGGGGMYVIVVVLPT
                     VQAEFGVARADASMPYTLLMIGFGVGGMFMGYLADRFGIMAPLMISAISLGLGFTAAA
                     MTGNIWTFTLVHGVLIGLLGCSASFAPLIADTSLWFVRRRGTAVAVCASGNYLGGAIW
                     PPIVEHFVNDVGWRQTYIYLGLFSFVAIVCLALLMRRRPPLTAPVTVLPAGKDQAAQA
                     SAAIGRAAQSRQASLPFGLSPNKAQALLCVAGLSCCVAMSMPQVHIVAYCGDLGYGVA
                     RGAEMLSLMLACGIISRLVFGVICDHIGGLRTLLLGSTLQMLALTLFLPFNGLVSLYV
                     LAALFGLFQGGIVPAYAIIVREYFPAEQAGSKVGMVIMFTMLGMALGGWMSGKIFDLT
                     GSYQAAFINGIAWNVLNLTIAITLFLRTRRRGYRPLPGGA"
     gene            complement(6391..6636)
                     /locus_tag="VIR04_10965"
     CDS             complement(6391..6636)
                     /locus_tag="VIR04_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9461307.1"
                     /translation="MDRLRSIEMFIRVAQGPSFSGAAQWLGIARSNVTRHVGWLEKSL
                     GTQLLARTTKSVRLTEAKPTQESGAPAARGNGNDRRK"
     gene            6691..6852
                     /locus_tag="VIR04_10970"
     CDS             6691..6852
                     /locus_tag="VIR04_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461308.1"
                     /translation="MMLTILVNRRFLPGEIMQIVASGASRVQQPAHLVPAMLGIMVHV
                     VQAAGIKME"
     gene            complement(6839..7669)
                     /locus_tag="VIR04_10975"
     CDS             complement(6839..7669)
                     /locus_tag="VIR04_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY9461309.1"
                     /translation="MRTQAEKAQAFRALHQRDGAFIIPNPWDVGTARLLANLGFEALA
                     STSAGYAFSMGLADYKVGRDRMLAHVAELVGATDLPVSADLENGFGDKLEDVAQTYRL
                     AAAAGLAGASIEDATGRIDRPIYEHALAMERVSAAVEAVRALPFPFTLTARAENYLHG
                     RPDLKDTIRRLQAYQDAGADVLYAPGLRTKADIAAVVTSVDLPVNVLMGGTGIALSAA
                     ELSDIGVKRISVGSSLSRAALGAFLRASREMLEHGTFTFGEAAVSYQDITEMLKATPS
                     "
     gene            7759..8628
                     /locus_tag="VIR04_10980"
     CDS             7759..8628
                     /locus_tag="VIR04_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY9461310.1"
                     /translation="MATQISAAASGQFQIGGDLRVNRLGYGAMRITGKGVWGEPADKA
                     EALRTLKRLPDLGVNFIDTADSYGPYVSEDLIHEALYPYTGLVVATKGALTRHGPDVW
                     RPLGRPEYLRQCVLMSLRRLGLERIDLWQLHRIDPKVPRNEQFDCIGQMQKEGLIRHV
                     GLSEVKIDDIKAAAPYFKVATVQNQFNLVDRGSEDVLNYCEKHGIGFIPWYPLAAGTL
                     ARPGALLNRVAKAHNAAPSQIALAWLLKRAPVVLPIPGTSKTAHLEENIAAANIQLTQ
                     DEFTALGRQKTAA"
     gene            complement(8640..9344)
                     /gene="msrA"
                     /locus_tag="VIR04_10985"
     CDS             complement(8640..9344)
                     /gene="msrA"
                     /locus_tag="VIR04_10985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY9461311.1"
                     /translation="MTNKRVWWAPFLLLAATVAVPFSGAATAAEARAVPAPVLDMAPA
                     GNSASPQVAVLAGGCFWGVQGVFQHVKGVESAVSGYAGGDAHTAQYETVSTGSTGHAE
                     SVQVTFDPHQISYGQILRIYFSVTLDPTQLNRQGPDTGTQYRSVIFPTNPQQAKVATA
                     YIKQLGAAHVFHAPIVTEVEPLKAFYTAEAYHQDFLTRHPTYPYIVINDLPKVRNLKQ
                     TFPTLYRSDPVLVTNL"
     gene            9543..9833
                     /locus_tag="VIR04_10990"
     CDS             9543..9833
                     /locus_tag="VIR04_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461312.1"
                     /translation="MNITTRAEREALATNGFYLHSDSSSEEKWSAALSQGSSSWVSIT
                     RTAATISVAMEQGAREFHYPMATFPPSHKTWLWSVLDIHRDIVDTMKLNTDD"
BASE COUNT         2121 a         2780 c         2982 g         2064 t
ORIGIN      
        1 aatattctgg gcttcgcccg cgctgtgcat gagcgctacc ccggcacgcc cattgcgttt
       61 tgcgcaatac taccttatga caggcgtctt gatctggaaa agctgggatg tataacgctg
      121 gaagatccga gcgtggcggt gcgcaccatc agcgcaatac tgaaacgaaa tcgcaacgcc
      181 cggccgaaag ccatcgcagt gcaaagcata aacctgcccg cccatacgcc cgccctgaac
      241 gaggccgaat cgttgctggc tttgaaacaa gcccatgtgc ccgtggttga attcaagctt
      301 gcatcttcca aagaggaagc tgcgtcgttg gtggagcaat tgaacgcacc tgccgcgatg
      361 aaaattcttt ccgccgatat tgcgcacaag agtgatatcg gaggcgtcaa gctttccgtg
      421 cgagggcccg acgaggcgcg cgccgcttac gatgcgatca tgtcttcagt cgcccgccat
      481 gctccccttg ccggacttga tggaatgctt attgcgccca tggcgccgga tggcgtggag
      541 tgcatcatcg gaatgcatag ggatccgacg ttcgggccgg tcatcatgtt cgggttggga
      601 gggatattcg tcgaaatatt aaaggacgtc agttttcgcc tggcgccgtt cgacaaggcc
      661 gaagcgctgg aaatgattca gcagacccgc gcggtcagtt tgctgaaagg cgcacgcggc
      721 cgcgcgccat cggatctgga cgcgctggct gaaagcctgg cggcactgtc aaacatggct
      781 tatgcggcgc gagaccgcat cagctccatc gacatcaatc cttttttggt gctgccggcg
      841 ggtcagggag ccatggcgct ggatgcgcta attttgcagg actaatggcc gggccggaat
      901 ccggccggta tggaaggcgc cgtgcgcggg atgatgattt cgtgtaaaag ggttcagttg
      961 gacgtctcga aacccaggag cctttatgtc tgaagcatca accatcaaga acaagattgc
     1021 gtccgccgca cagttattgc tcaatgcgcg cagcacgcaa aaattgctgc ccgagcttcc
     1081 ctctgaatat cgccccggca attccgccga agcgtatgcc atccaggacg cggtcgtcgc
     1141 gcaactcggg cccataggcg gatggaaggt cggcgccggc agcgccactg ccgaaccgaa
     1201 ttgctctcct ttgccaggct cgctgatttt caatggttcc ggttcgtttt catcgcgcgt
     1261 gtatacgctg cggggtattg agggggaaat cggggtgcgc atgaagcgcg atcttccgcc
     1321 cagagccgaa ccgtactcga ttcccgatgt aataagcgcg gtgggctccg tgcaccctat
     1381 gattgaagtc gtcgaatcgc gtttcacgga ccgtggaaaa gttgatccgc tgtccgcgct
     1441 ggccgacgcc gccagcaatg gcgctcttgt ttatggcgaa ggtcgcacgg acaaccttgc
     1501 cattgatcag gcctcgcaat tgttcgagct gtatttcgac tcgaaaaaag tagtcgagct
     1561 tgtcgggggc aactcggcgg ggaatatatg gcggctgatg gcctggctgg cgaaccacgt
     1621 agcggcgcgc tgcggcggtt tgcgcgcggg gcaaatagtc acgaccggat cctgcacggg
     1681 ggtgacctac gccaagcccg gcgccagggt gaaggtgtcg tccccaggtc tgggcaaggc
     1741 cgagttgtcg ttttccgtct tatgaaagtg cccaaaagat tggcgccatt acttgacgaa
     1801 gggcttatcg acgaggtgct tggtcagttg atgagcggca aggaagcgac ggtgtacatc
     1861 gtgcgcagcg gcaatgagac gcggtgcgca aaggtctaca aggatgtgaa acagcgcagt
     1921 tttcggcagg cgacgtccta tcgcgacgga cgcagtgtca agaacagccg tcaggcgcgc
     1981 gcgatggaga aaggcacccg ttatggccgc gaggtgcagg agcagttgtg gcagacggcc
     2041 gaggtggacg ccctgttccg gctgaccaat gcgggcgtgc gtgtgccgca accctatatt
     2101 tgcaccgacg gcgtattgtt gatggaattg gtgctcgacg cggcgggcga cgttgcgccc
     2161 cgattaaacg atgtcgattt aacgcaagag caggcgcttg atttgcatgc gcggcttctt
     2221 aaccaggtgg ttcgcatgct ttgcgcgggc gtgattcatg gcgacctgtc cgaatacaat
     2281 attctgctgg cggctgacgg gccggtcatt atcgacttgc ctcaggcagt cgacgcggcg
     2341 gccaataatc aggcgaaagc catgctcgag cgcgatgtcg ataacctggc aacgtatttc
     2401 gggcgtttcg cgccgcaact gcttgtggca aattacggta aagagatctg ggcgctgttc
     2461 gaggcaggta atctgagcgc cgatatggag ctgaccggcc gcgttgagct cgatacgcgt
     2521 ccggtcgatc ttgacggttt gatggacgat atcgaagacg ctcgtttcga agaagatgcg
     2581 cggcggcgcc gcgcggcaga actaaggagc tagcatgaaa atagtaatgg ttttgacctc
     2641 acatgaccgg cttggcgaca ccggacagaa aacgggattt tggctggaag agttcgccgc
     2701 tccgtactac gttttcaaag acgcgggggt ggatattaca ttggcgtcgc catcaggcgg
     2761 ccagcctcct gtcgatccca agagcgatgc cgccgattct cagactgacg ccacccgccg
     2821 tttcaaggca gaccccgcgg cacaggcggc gttggctcat acccgcaagc tatccgacgt
     2881 tttggccacc gactttgatg ccgtgttcta tccaggcggt catgggccat tatgggatct
     2941 ggcggaaaat gcaagctcca ttgccttgat cgaagccaca ttcgccagcg gcaaacccgt
     3001 cgtggcggtt tgccatgcgc cgggtgtgtt gcgccatgcc aaggcgccag acggcacgcc
     3061 gctcgtccgc ggaaagaccg tgaccggttt cacgaacaca gaagaagagg cggtcggatt
     3121 gacccatgtt gtgccctttc tggtcgagga cgcgctgaaa aaaaacggcg gccactactc
     3181 caaggcgggt gattggcaat cgcatgtggt gtcggatggc aacctgatta ccggacaaaa
     3241 tcccgcttca tcggtagcgg cggccaaggc cttgctggac ctgcttgggc acgcgaacca
     3301 agggattgcc cgctgacgga atcagactaa ggcggtaaac tgttgcgggt catcgagact
     3361 atttcccggc atagcaacat ttcggaattc cataggaaga aaatgagtac agccaaacat
     3421 ttacggtccg cgcacatcac cgagggcgtg gagcgcgcgc caaatcgctc catgtattat
     3481 gcgatgggtt acaccgaaga ggatttcaag aagcccatgg tgggcgtcgc caatggtcac
     3541 agcaccatca cgccctgcaa tagcggattg cagaagctcg ccgatgccgc gatcgaagca
     3601 attgaagagg ctggcggaaa tgctcaggtg tttggcactc cgaccatttc agacggaatg
     3661 gcgatgggca ccgaagggat gaagtactca ttgatttcac gcgaagtcat tgcagactgc
     3721 gttgaaacct gtgtgcaggg ccagtggatg gatggcgtgc tggtgattgg cggttgcgac
     3781 aaaaatatgc cgggcggcat gatgggcatg ctgcgcgcca atgcgcctgc catttatgtt
     3841 tacggcggca cgatcctgcc gggatgctat cagggcaagg atttgaatat cgtcagcgtg
     3901 ttcgaagccg ttggcgaaca cgctgccggc cgtcttagca ccaccgattt gcgtcagatt
     3961 gaattgcacg ccatacccgg ctgcggttca tgcggcggca tgtacaccgc caacaccatg
     4021 agctcggcgt tcgaagccat ggggatgagc ttgccgtatt cttcgacgat ggccaacgag
     4081 cacgccgaaa aagtcgaatc agcaaaggaa tcggcgcggg tattgttgcg cgcgattgaa
     4141 ctggatctta agccgcgcga tatcgtaacg cgcgagtcca ttgaaaacgc ggtcagcgtc
     4201 attatggcca cgggcgggtc gaccaattcg gttttgcact ttcttgccat tgcgcatgcg
     4261 gccggcgttc agtggacgat tgacgacttc gaacgggtgc gccagcgcgt gccggttttc
     4321 tgcgacctca agcccagcgg cgaatacctc gttgtcgatt ttcaccgggc gggcggcatt
     4381 ccgctggtca tgaaaatgtt gctcaatgcg ggtcttttgc acggcgaatg catgaccatt
     4441 accggcaaga cgatcgccga aacgctgaaa gacgttcccg atacgccggc tgcgaatcag
     4501 catgtcatcc acacgattga caagccgctc tatcctcagg gtcacctggc catacttaaa
     4561 ggcaatcttt ctcccgaagg ctcggtggca aaaatcaccg gtctgaaaaa cccggtcatg
     4621 accgggccgg cacgggtgtt tgaagacgaa cagtcggggc tgaaggccat tcttgacggc
     4681 aagatcaagg cgggcgacgt gatggtattg cgttacctgg ggcccaaagg cggcccgggc
     4741 atgcctgaaa tgctcgcgcc cacgggcgcg ctggtaggtg cggggctggg cgactcggtt
     4801 ggattgatca ccgacggccg gttttcgggt ggaacctggg gcatggtggt cgggcacgta
     4861 gcccccgaag cggcggtggg cggttgtatc gcgctggtac aggaaggcga ttcaatcacc
     4921 atcgacgccc atcatcaatt gctacagctt aacgtgcccg atgacgagat tgccgcgcgt
     4981 cgcgcgaaat gggtggcgcc caaaccgcgt tatctgcgag gcgttcaggc caagtttgcc
     5041 ttcaacgttt cgaccgccag cctgggcgcc gtgctggaca gtttctgact ctgatagccg
     5101 gcacacgttc aggcgccgcc aggcagggga cggtatcccc tgcggcgggt ccttaggaat
     5161 agcgtaattg caatagtcag gttaagtaca ttccaggcaa tgccgttgat gaacgccgcc
     5221 tggtaggagc ctgtcaggtc aaagattttg cccgacatcc atccgcccag cgccatgccg
     5281 agcatggtga acatgatcac catgccgact tttgacccgg cctgttccgc gggaaaatat
     5341 tcccgcacaa taattgcata cgccggcaca atcccgccct gaaacaggcc gaacagcgcg
     5401 gcaagcacgt atagcgatac aaggccgtta aatggcagaa aaagtgtcag cgccagcatt
     5461 tgcaacgtcg agcccagcaa tagcgttcgc aagccgccga tgtgatcaca aatgacgcca
     5521 aagaccagac ggctgataat gccgcaagcc agcatcaggg aaagcatttc agcgccgcgc
     5581 gcaaccccgt agcccaggtc gccgcaatac gcgacgatat gcacttgcgg catggacatg
     5641 gcgacgcaac aacttagccc ggcaacacac agcaatgcct gggctttgtt gggcgatagc
     5701 ccgaaaggca aggacgcctg ccgggattgc gcagcgcggc caatggccgc cgaggcctgt
     5761 gccgcctgat cttttcctgc aggcagcacg gtgacgggtg cggttaaagg cggcctgcgc
     5821 cgcatcagca atgccaggca gacgatggcg acgaagctga acagccccag atatatatag
     5881 gtctggcgcc acccgacgtc gttgacaaag tgctctacga tgggcggcca tatcgctccg
     5941 cccagatagt tgccgctggc gcaaacggcc acggccgttc cgcggcggcg cacgaaccaa
     6001 agcgacgtgt cggcaatgag cggcgcgaaa gacgctgaac agcccaacag tccgattaac
     6061 acgccgtgca ccagcgtgaa cgtccagata ttgccggtca tggccgctgc cgtgaagccc
     6121 agtcccaggc tgattgcgct aatcatcaat ggcgccatga tgccgaagcg atcggcaagg
     6181 tatcccatga acatgccgcc cacgccaaat ccgatcatca gaagtgtgta aggcattgag
     6241 gcgtcggcgc gcgcgacccc gaattcagcc tggaccgtgg gcagtacgac cacaatcacg
     6301 tacatgccgc cgccgccaat tgtcatcagc gtcagcgtga gcagaaggcg cgcaatcgca
     6361 tagggcgagt cgaccaactc ggcgcttgtc ttatttgcgg cggtcattgc cgttgcccct
     6421 tgcggcgggt gcgccgcttt cttgcgtcgg ctttgcttcg gtcaggcgca cgcttttggt
     6481 cgttcgggcc aacaattgag tgcccagcga tttctccagc cagccaacgt gcctggttac
     6541 attgctcctg gctattccca accattgcgc ggcccccgag aaactaggcc cttgagcgac
     6601 ccggataaac atttcgatgc tgcgaagccg atccattttt gtttctcttc agggaacaat
     6661 aagtcccggg ttctattcta tccacacaga atgatgctca ctatactggt caaccgtcgt
     6721 ttcttgccag gagaaattat gcagatcgtg gccagcggcg cgagccgggt gcaacagcca
     6781 gcccatttgg tgccggcgat gttgggtatt atggtgcacg tcgtccaggc agccggcatc
     6841 aagatggagt agctttcaac atctcggtaa tgtcctgata gctaactgcg gcttcgccaa
     6901 aagtgaatgt gccatgttcc agcatttccc tgcttgcccg caagaaggct cccagcgctg
     6961 cccggcttag cgagctgcct acgctgatcc gtttgacgcc aatgtcggat aattcagccg
     7021 cgcttagcgc gatgcctgtc cctcccatca ggacattcac tggcaggtcg accgaagtca
     7081 ccactgcggc gatgtcggct ttggtgcgca gacccggcgc gtacaacacg tcggcccccg
     7141 cgtcctggta ggcctgaagg cgcctgatcg tgtctttcag atcgggccgg ccatgaaggt
     7201 aattttcggc gcgagcagtc aaggtgaagg ggaaaggcag cgcacgcacg gcttctacag
     7261 cggcgcttac gcgttccatt gccaacgcat gttcatagat cgggcggtcg atccggcctg
     7321 tggcgtcttc aattgacgct ccggcgagcc ctgcggcggc ggcaaggcga taggtttgcg
     7381 cgacatcttc cagtttgtcg ccaaagccat tttccaggtc ggcgcttacg ggcaggtcag
     7441 tggcgccgac caactcggcg acatgcgcaa gcatgcgatc acggccgacc ttgtagtccg
     7501 ccaaccccat tgaaaaggcg taccccgcgc tggtgcttgc cagcgcttcg aaacccaggt
     7561 tggccagaag ccgcgcggtg ccgacgtccc atgggttggg aatgatgaaa gcgccatctc
     7621 tctgatgtaa cgcgcggaaa gcctgcgctt tttctgcttg agttctcatt gaatgaagct
     7681 cctgttggat cgtcgtcagg ttgactgagt acactaatat atttgcgggt attcctactt
     7741 taatcaggag aacgaatcat ggcaactcag atctcagccg ctgcttcggg acagttccag
     7801 ataggcggcg acctcagggt caatcgcctg ggctacggcg caatgcggat taccggtaaa
     7861 ggcgtctggg gcgagccggc ggacaaggct gaagcgttgc gtacgctcaa gcgtctgcca
     7921 gaccttggcg tgaattttat cgatacggcc gatagctacg gcccctatgt cagcgaggac
     7981 ttgattcatg aggcgctgta tccctacacg gggctggttg tcgcgacgaa aggcgcatta
     8041 acccgccacg ggccggatgt gtggcggccg cttgggcgcc ccgaatattt gcgtcagtgt
     8101 gtattaatga gcttgcgccg attggggttg gaacgaattg atctttggca attgcatcgg
     8161 attgacccca aggttccgcg caacgaacaa tttgattgca tcgggcaaat gcaaaaagag
     8221 gggctcattc gccatgttgg acttagtgaa gtcaaaatcg atgacatcaa ggcggctgcc
     8281 ccttacttca aggttgccac ggtgcaaaat caattcaatc tggtcgatcg cggcagtgaa
     8341 gacgtcctga actactgcga gaaacatggc attggcttta tcccatggta tccccttgcc
     8401 gcgggtacgc tggcccggcc cggcgcgctt ctgaacagag ttgccaaggc ccacaacgca
     8461 gcccccagtc agatcgcgct ggcgtggctg cttaaacgcg cccctgtcgt attgccaata
     8521 cccggcacat cgaaaacggc acatctggaa gaaaatatcg ctgccgcgaa tattcagttg
     8581 acgcaggacg aattcacggc gctcgggcgg caaaagacag cggcgtgaat caatacggct
     8641 tataaattgg ttaccaatac gggatctgat cgatacaggg ttgggaacgt ctgtttcaga
     8701 ttccgcactt tcggcaaatc attgatgacg atgtagggat aggtgggatg acgggtcaga
     8761 aaatcctgat ggtaagcttc ggccgtatag aacgccttta gcggttccac ttcagtcaca
     8821 ataggcgcgt ggaacacatg ggccgctcca agctgcttga tataagcggt ggccaccttt
     8881 gcttgttgcg ggttcgtggg gaagatgacc gagcggtatt gcgtgccggt gtctggcccc
     8941 tgccggttaa gctgggtcgg atcaagcgta acggaaaaat aaatccgcag aatttgccca
     9001 tagctgatct gatgtgggtc gaaggtgacc tgcactgact ctgcgtggcc tgtggatcca
     9061 gtgctgacgg tctcgtactg tgccgtgtgc gcatccccgc ccgcatatcc cgatacggct
     9121 gactcgacgc ctttcacgtg ctggaatacg ccttgcacgc cccaaaaaca gccgccggcc
     9181 aggacggcca cctgcggcga tgctgaattg ccggccggcg ccatatcgag cacgggcgct
     9241 ggcacggccc tggcttcggc ggccgttgca gcgccggaaa aaggaacggc aaccgttgcg
     9301 gccaacaaaa gaaacggcgc ccaccatacg cgtttgttcg tcattacctt ctcctgtatg
     9361 ccgagtggcc tgtcaggcgt cattgcacgg cttaaaggcg ttacgactgt tattgcagaa
     9421 aatcgttccg caattcacga attgtatcta tgctgcggct tgtcgagtta cactttaaag
     9481 aagtcaagga tcaactatgt cagtacaatg ctgccatccc gttgtattct tcccttgctt
     9541 ttatgaatat tactacccgt gcagagcgtg aagcgctcgc aacgaatggc ttttatctgc
     9601 attcggactc atcatccgag gaaaagtggt ctgccgcact gtcgcagggg tcctcgtctt
     9661 gggtaagcat tacccgcacg gccgccacca tctcggttgc catggagcaa ggcgcccggg
     9721 aatttcacta tcccatggcc accttcccgc ccagccacaa aacgtggctc tggagcgttc
     9781 tggatattca tcgagacatt gtcgatacga tgaaattgaa taccgacgac tgaaatcgct
     9841 tcgggcgcgg ctttgtcagg ttaatggcaa cataaccttg tcttgatagg ctggccgttg
     9901 cgccaggcgg ttgcaccacg catcgacgtg tgtcagcggc gggtgct
//
LOCUS       DATCXU010000490         5945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_449552,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000490 DATCXU010000000
VERSION     DATCXU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449552"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VIR04_10995"
     CDS             complement(<1..481)
                     /locus_tag="VIR04_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9461313.1"
                     /translation="MARFTRRRSLVRQTRSPQPLDSTDLMPSDIPLSANPYTHGIASF
                     VSGLQYESIPPEVLTRIKLLMLDSIGCAIYGANLPWSRILQKTLSMVDTTEGCTVWGT
                     GQKLSSPHAALINGTQVQAFELDDVHRQGVLHVGAVVLPALISIAESRPGMSGRDFMT
                     "
     gene            complement(451..1470)
                     /locus_tag="VIR04_11000"
     CDS             complement(451..1470)
                     /locus_tag="VIR04_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY9461314.1"
                     /translation="MGEIIVLKPPTLPGWTATASPFHSSELTIQERVGVRTRIDISAR
                     RAGIRDYMPDQHRLFFSERPFLLLGAVDADGQPWPTIMVGKPGFVTTPDARTLHIAAT
                     LLPGCPLQGQLRVGTSLGTLGIQFATGRRNRANGVLTAVQPTGLTLAVRQSYGNCRKY
                     IQAREPTLLGIGNTAATPAVLDTGRLSDADRNLISRADTFFIASACFDEHNPIAQGAD
                     VSHRGGRPGFIRIDDDDTLTIPDFTGNFFFNTLGNLLHDPRAGLLFIDFESADLLYVS
                     ADAHIIWDGPQVNAFLGAQRLVQFHLRKIRRVANVLPISWSPVHYSDKLAGTGIWQDL
                     LGAGL"
     gene            complement(1458..2099)
                     /locus_tag="VIR04_11005"
     CDS             complement(1458..2099)
                     /locus_tag="VIR04_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY9461315.1"
                     /translation="MPINKPVQPIRVYGAELSGHTHRVLLFLSLLGLPYEFIKVDLAA
                     GAHKRPEFLAKNPFGQVPVIEDGNVVLFDSNAILVYLATKYDDGAWLPRDPLGAATVQ
                     RWMSLAAGHIFNGPAAARMVTVFGAPLDHERAKTLSTALFKVLDAELRGRDFALGKAP
                     TLADVTAYAYIAHAPEGGVSLEPYPNIKAWLQHVEELPGFLPMPTSKAGLWAK"
     gene            complement(2241..3272)
                     /locus_tag="VIR04_11010"
     CDS             complement(2241..3272)
                     /locus_tag="VIR04_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461316.1"
                     /translation="MPVLLPTTLVGSYAQPDWLIDRAKLGERFPPRVRAKELWRVGPE
                     WLEQAQDDATLLAIRAQEDAGLDILTDGEMRRESYSNRFATALDGVDIDNPGTALDRS
                     GHPNPVPRVVGKIRRKHPVQVRDIKFLRAHTDRQIKITVPGPFTMTQQAQNDFYASEE
                     EMAQDYAAAVNEEIKDLFAAGADIVQIDEPYLQARSEKARAYGVRTLNRALEGVVGTT
                     AVHICFGYAAIIHERPVGYAFLAELESCLANQISVETAQSKLDCAILEKLPSKTILLG
                     VLDLSTHNIETPEIVAERIRRALPHVPAERLVIAPDCGLKYLPRDVAYGKMKAMADGA
                     RIVRTELSR"
     gene            complement(3361..4578)
                     /locus_tag="VIR04_11015"
     CDS             complement(3361..4578)
                     /locus_tag="VIR04_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9461317.1"
                     /translation="MKKATPRWLIPVAAIFILQTTTSFLARFIPIISPAMSDEFGWGG
                     SSIGYLTAIASFGGFAVLLMAGSSLFRQIGGIRVLQFSLILGAASLALFLNSSLAVAL
                     MACFVMGLSNGAANPAGSEVLQRFSPPEKRNLMFSVKQAGVPLGGVIAGLAIPAIVIL
                     TSWRSALFAGACLIVLPTLLTWRMCAGIDEHSSSKRRLFTMPRLSSLRTLTVPLQSLT
                     RGPGLLKMSVVGSLFAVAQSCWFTFTVIYLIDGLGFSLGLAGAIFAIMQAGGVFGRIA
                     LGWLTDRMHSPTVTLSGTAIVSAITTVLLGLSRPTWPLWTIIVLAFIAGCSVSSWNGV
                     QIAEVARRSPAELISETSAGSSILINLTNVIVPTAFAAFVAISGRYDYAFMCAGLSTL
                     LVIAFLPRNERPA"
     gene            complement(4653..5891)
                     /locus_tag="VIR04_11020"
     CDS             complement(4653..5891)
                     /locus_tag="VIR04_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY9461318.1"
                     /translation="MPSECGSQTQQGTRNDASTVAVSATTDTDEMVTKGRYLASVGDC
                     MACHTAQGGKPYAGGKTIPTPFGALYGPNITPDEQTGIGRWSADDFWHALHNGKAPDG
                     SLLYPAFPYTEYTRISRADADALYAFLKTVKPVRQASRPPELKFPYNQRRLMAGWRAL
                     YFHPGVQKPDAGQSVQWNRGRYLVNGIGHCAACHAPRNSLGATSPADGLSGGMIPVLN
                     WYAPPLTGDAVAGMGKWSAQDIAALLKTGISAHSTAVGPMGEVVLGSSQFLTDDDALA
                     MGVYLKTLPASASHTKNADPAPSAASLERGKKLYTQHCAQCHQDSGEGSGSAWPPLAG
                     NPTVNAPSAINAIRVVLDGGFAPPTGANPRPHSMPPFGATLGDNEVAMLVTYIRNSWG
                     NDAGSVSALDVNRIREAGPR"
BASE COUNT         1221 a         1634 c         1851 g         1239 t
ORIGIN      
        1 cagtcatgaa gtcgcggccg ctcattcccg gtcgcgattc agcaatcgag attagtgcgg
       61 gcagtacaac cgcaccgaca tgcagcacgc cctggcggtg cacgtcgtcc agttcgaacg
      121 cttgcacctg cgttccattt atcagcgccg catgaggcga ggaaagtttt tggccggttc
      181 cccatacggt gcagccttcg gtcgtatcga ccatgcttag ggtcttttga aggatacggc
      241 tccatggcag gtttgcgccg tagatcgcgc aaccgatgga atcgagcatc agaagcttga
      301 tgcgcgtcag cacttcgggt ggaatggatt cgtattgaag accggagacg aagctggcga
      361 tgccgtgtgt gtatgggttt gccgaaagcg ggatgtcgga gggcatgagg tcggtggaat
      421 caagtggttg aggggatcga gtctgccgga ctaaagaccg gcgccgagta aatcttgcca
      481 tatcccggtt ccggccagtt tgtcggaata atgcacaggc gaccagctta tgggcaagac
      541 gttcgccaca cggcgaattt tgcgcaggtg aaattgaacg agccgctggg cgcccaggaa
      601 tgcgttgact tgcgggccat cccagataat atgcgcatcg gccgatacgt acagcaggtc
      661 agcggactca aaatcaatga agagcaggcc cgcgcgcgga tcgtgcagca agttgcccag
      721 cgtattgaag aaaaagttgc cggtgaaatc cggaatggtc aatgtatcat cgtcgtctat
      781 acgtatgaaa cccgggcgcc ctccgcgatg cgagacgtcc gcgccttgcg ctatcgggtt
      841 gtgttcatca aagcaggccg aggcaatgaa aaaagtgtcc gcccggctga ttaaattcct
      901 gtcggcgtcg ctaagccggc cggtatccag aacggccggc gtcgcggctg tattgccgat
      961 gccgagcagg gtgggttctc gcgcttgtat gtatttgcgg cagttcccgt aactttgccg
     1021 cactgccaat gtcaggccgg tgggttgcac ggcggtcagc acgccgttcg cgcgattgcg
     1081 ccggccggtg gcgaattgaa ttcccaaagt gcccaacgat gttccgacgc gcagttgccc
     1141 ctgtaatggg cagcccggaa gtagcgtggc ggcgatgtgc agggttcgcg catccggcgt
     1201 tgtgacaaag ccgggtttgc cgaccatgat cgtcggccat ggctggccat cggcatcgac
     1261 tgcgccaagc aacaggaagg ggcgttcgct gaaaaacagc cggtgctggt cgggcatgta
     1321 gtcgcgaatg cccgctctgc gcgcactgat gtcgattcgc gtgcgcacgc ccactcgctc
     1381 ctggatcgtt agctcgctgc tatggaacgg cgatgccgtc gccgtccatc ccggtagcgt
     1441 gggcggtttt agcacgatta tttcgcccaa agacccgcct tgcttgttgg catgggaagg
     1501 aatcccggaa gctcttccac atgttgcagc caggccttga tattgggata gggttcaagc
     1561 gatacgccgc cttcgggcgc gtgggcaata tacgcatagg ctgtcacatc ggctaacgta
     1621 ggcgctttgc ccagggcgaa gtcgcggccg cgcaactcgg catccagcac cttgaaaagc
     1681 gctgttgaaa gcgttttggc ccgttcgtga tccagcgggg ctccgaatac cgtgaccatt
     1741 cgcgccgcgg cgggaccatt gaatatatgg ccggccgcga gggacatcca gcgttgcacg
     1801 gttgctgcgc ccagcgggtc gcgtggcaac cacgcgccgt cgtcgtattt ggtcgccaga
     1861 taaaccagga tggcattcga gtcgaatagc accacgttgc cgtcttcaat aacgggcact
     1921 tgtccgaacg gatttttggc cagaaattcg gggcgtttgt gagcgccggc ggcgagatcc
     1981 actttgatga attcataggg cagtcccaac aggcttagaa acagcaaaac gcggtgtgtg
     2041 tggcctgata gttcggcgcc gtaaacgcga atgggttgga ctggtttgtt tattggcatt
     2101 gttaactcct gttaaacgga tgcttcctgg aagaagcttg tctaaaggat agtcctatta
     2161 tgtttcacgc agcacaatac ggcccgacag cgaagcgccg atagccgcat agcggcttat
     2221 cgggatccgg taaaaattta ttagcgggac agctcggtgc gaacgatgcg ggcgccatcg
     2281 gccatggctt tcattttgcc ataggcgaca tcgcgcggca ggtatttgag accgcaatcg
     2341 ggcgcaatga ccagccgttc cgccggcacg tgaggcaagg cccggcggat gcgttcggcc
     2401 acgatttcag gcgtttcgat attgtgcgta gacagatcca ggacgcccag caaaatcgtt
     2461 ttggatggaa gtttttccaa aatggcgcag tcgagtttcg attgagcggt ctccacggat
     2521 atttggttgg caagacagct ttccagttcg gccagaaaag cgtatcccac agggcgctcg
     2581 tgaataatgg cggcatagcc aaaacagata tgcacggccg ttgttccgac aacgccttcc
     2641 agagcccggt ttagcgtgcg cacgccatag gcccgcgcct tttcggatcg ggcctgcaag
     2701 tagggctcgt caatctggac aatgtcggcg ccggcggcga acagatcctt gatttcttcg
     2761 ttgacggctg ccgcatagtc ctgggccatt tcttcttcgg aggcgtagaa atcgttttgc
     2821 gcctgttggg tcatggtgaa ggggccgggc acggtaatct tgatttggcg atcagtgtgg
     2881 gcgcgcaaaa atttgatgtc acgcacttgc acgggatgtt tgcgccggat tttgccaacg
     2941 acgcgcggca cgggattggg gtgtccgctg cgatccagcg ccgtgccggg attgtcgata
     3001 tcgacaccgt ccagcgccgt ggcaaagcga ttggaatagc tctcccgtcg catttcaccg
     3061 tcggtcagta tgtccagtcc ggcgtcttcc tgcgcgcgaa tagccagcag cgttgcgtcg
     3121 tcctgggcct gttccagcca ttccggtccg acgcgccaaa gctctttggc gcgaacgcgg
     3181 ggaggaaaac gctcgcctag cttggcgcgg tcgataagcc agtcgggctg tgcatatgag
     3241 ccaaccaggg ttgtgggtaa aagtacgggc atgatacgtt ccagtaagca gcggagtgaa
     3301 gcgttattga cgatcgcctg cactcattaa aaaataggat gtcacgggat aatactcgat
     3361 ttaggctggc cgttcgttgc gcggcaggaa tgcgataacg agcagcgtgg agaggccggc
     3421 gcacataaag gcgtaatcgt atcggccgct tatcgcaacg aaagcagcga atgccgtggg
     3481 cacgattacg ttggtcaggt taatcagaat gctcgatccc gcagacgttt cggaaatcaa
     3541 ttccgcagga gatcgtcgcg ccacttcggc gatttgtacg ccattccagc ttgaaaccga
     3601 gcatcctgcg ataaatgcca ggacgataat ggtccatagt ggccaggtgg ggcggcttag
     3661 ccccagcaac acggtggtga tggcggatac gattgcggtt cccgatagag tgacggtggg
     3721 cgagtgcatg cggtcggtaa gccagccgag cgcgatgcgg ccaaatacgc cgcccgcttg
     3781 catgatggcg aaaatggcgc cggccagtcc cagtgagaag ccaagcccgt cgatcaggta
     3841 aatgacggtg aaagtaaacc agcagctttg cgccaccgca aacagacttc cgaccactga
     3901 catctttaat aagccggggc cgcgcgttaa cgattgcaac ggcacggtga gcgtgcgcaa
     3961 cgaactgagc cgcggcattg tgaagagccg tcgttttgag ctggagtgtt catctatccc
     4021 ggcacacatg cgccaggtca gcagagttgg aagaacgatc aggcaggcgc cggcgaaaag
     4081 ggcggagcgc caactggtca gaatcactat ggcggggatg gcaagcccgg ctatgacgcc
     4141 acccagcggc acgccggcct gtttgactga aaacatcagg ttgcgttttt ccggcggcga
     4201 aaatcgttgc agaacttcgc tgccggccgg gttggcggct ccgttgctta accccatcac
     4261 aaagcacgcc atcaacgcta cggccagact tgaattcagg aaaagggcca ggctggctgc
     4321 gcccaggatc agactgaatt gcaatacgcg aattccgcct atttgcctga acagacttga
     4381 gcccgccata agaagcacgg caaagccgcc gaatgatgca attgccgtca aatagccaat
     4441 tgagctccct ccccatccga attcgtcgga catggcggga gaaatgatgg gaatgaaccg
     4501 ggccaaaaat gacgtggtgg tttgaagaat gaaaattgcc gctacgggta taagccagcg
     4561 tggcgtcgcc tttttcattg agtgtccctt aaattatttt tccggattat gggttaaagg
     4621 agcattgccg agtttggcga ggcgcaatca gttcatcgcg gccccgcttc gcggatgcga
     4681 ttgacgtcca gcgctgagac ggatcccgcg tcgttgcccc agctattgcg aatataagtc
     4741 accagcatcg ccacttcatt atcgccgagc gttgcgccga aaggcggcat gctgtgcggg
     4801 cgtgggtttg ccccggtcgg aggcgcgaaa ccgccgtcaa gcaccacacg tatggcattg
     4861 atcgcggagg gcgcgttcac ggtgggattg cctgccaggg gcggccatgc cgagccgctg
     4921 ccctcgcctg aatcctgatg gcattgcgca caatgctggg tataaagctt tttccctctt
     4981 tccagcgatg ccgcagacgg cgccggatcg gcgtttttgg tgtgggacgc agaagcggga
     5041 agggttttaa ggtacacacc catcgccaga gcatcgtcgt cggtaaggaa ttgcgaactg
     5101 cccagcacca cttcgcccat ggggccgact gcggtggaat gtgccgagat gcccgttttg
     5161 agcagagcgg caatgtcttg tgctgaccat ttccccatcc ccgcaacggc atcgcctgtc
     5221 aggggcggcg cgtaccagtt caataccgga atcattccgc ccgacaatcc gtcggcgggg
     5281 cttgtcgcgc ccaggctatt gcgcggtgcg tggcaagcgg cgcaatgacc tattccgttc
     5341 accaaataac ggccgcggtt ccattgcacg gactgacccg cgtccggctt ctggacaccg
     5401 ggatgaaaat acaatgcccg ccatcccgcc ataagacggc gctggttgta ggggaatttg
     5461 agttccggcg gacggctggc ctgtctgaca ggcttgaccg ttttcaggaa ggcatacagc
     5521 gcgtcggcat ccgcccgact tatgcgggtg tactcggtat aggggaacgc cggatacagc
     5581 aggctgccgt ccggcgcctt gccgttatgc agcgcatgcc agaaatcgtc cgcgctccag
     5641 cgtccaatgc cggtctgttc atcgggcgtg atgttgggtc cgtagagcgc gccaaacggc
     5701 gtcgggatgg tctttccgcc cgcgtaaggc ttgccgccct gcgcggtatg acaggccatg
     5761 cagtcgccca cgctggccag gtaccgcccc ttagtcacca tctcgtcggt atcagtggtt
     5821 gcgctgaccg ccacggttga tgcgtcgttg cgggtgccct gttgtgtctg gctaccgcat
     5881 tcggaaggca attcgacgga tccctggggt tgcggcgcat agggctcgac gatcggctgc
     5941 gaggc
//
LOCUS       DATCXU010000491         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_610414,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000491 DATCXU010000000
VERSION     DATCXU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610414"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="tsaB"
                     /locus_tag="VIR04_11025"
     CDS             complement(<1..855)
                     /gene="tsaB"
                     /locus_tag="VIR04_11025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY9461319.1"
                     /translation="MDLQLLAFETSSRSCSVALLSLVQGRLTARCSAHDATGEHAEVL
                     LPMADQLLQQACVARSQLTAVAFGQGPGGFTGLRVACGVAQGIAFALALPVIPVGSLL
                     AVAALDDTLEASSSITASARLVRVVVQDARMGEVYLAAYRFDAATESDLAGWHVVQAP
                     ILLNASDVGNWFEQSVGRWRDGSGVGASIRLMGDALTVYPELMDLTSFRTGSANPDTI
                     VLAGPLRPGADTIARLALSAWRRGQWVTPELAAPRYVRDKVAYTTRERARGLGGNPKV
                     GGVPVAIDQ"
     gene            1103..1837
                     /gene="ompR"
                     /locus_tag="VIR04_11030"
     CDS             1103..1837
                     /gene="ompR"
                     /locus_tag="VIR04_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY9461320.1"
                     /translation="MNTQNQSLSRKILVVDDDPRLRDLLRRYLTEQGFNVFVAEDGKE
                     MTKLWQREHFDLLVLDLMLPGEDGLSICRRLRGGHDNTPIIMLTAKAEEIDRIVGLEM
                     GADDYLSKPFNPRELLARVNAILRRRGTEEHPGAPSQENESIEFGPYVLNLSTRTLTR
                     NDEQVPITTGEFSVLKVFARHPKIPLSRDKLMELARGREYEAFDRSLDVQISRLRKLV
                     EPNPSKPVFIQTVWGLGYVFVPDGGH"
     gene            1873..>2570
                     /locus_tag="VIR04_11035"
     CDS             1873..>2570
                     /locus_tag="VIR04_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY9461321.1"
                     /translation="MSQPKKRLKKATSRLRVGLFGRSFLLLAALMLVSLGAWLQVFFS
                     MEEGPRALQMAQRVTTAVSITRSAIIYAPPSVRPSLLLDLATKESLRVQPREEPDVLQ
                     PLPDSNYWDRVAAEISERLGPKTQIMWTVNQTPGIWVSFDINNDKYWLVFDREQLGLT
                     AGAEWFGWGATALLLALIGAAVSVRFVNRPLAQLAKVAQQLARGETPSPLPEKGPLEI
                     REMNTAFNRMARDL"
BASE COUNT          574 a          811 c          684 g          501 t
ORIGIN      
        1 ctgatcaatc gccaccggca cgccgcccac tttgggattg ccgcccagcc cgcgcgcgcg
       61 ctcccgggtg gtgtaggcca ctttatcgcg cacataccgc ggcgccgcca attccggcgt
      121 cacccactga ccgcgtcgcc aagccgaaag agccaacctg gcgatcgtgt cggcgccggg
      181 gcgtagtgga cccgcgagga cgatggtgtc cggattcgcg gagcctgtcc ggaacgatgt
      241 caagtccatc agctccgggt aaacagtcag tgcatcgccc atcaaccgga tcgatgcgcc
      301 gacgcccgat ccgtcacgcc agcggccaac cgattgctca aaccaatttc caacatcgct
      361 ggcattaagc aatatcggcg cttgcacgac gtgccagcca gccagatccg actccgtggc
      421 agcatcgaag cgataagcgg ccaaatatac ttcgcccatc cgcgcatcct gcaccaccac
      481 acgaaccaat cgcgcagacg ccgtaatgga cgacgatgct tccagcgtat catcgagcgc
      541 cgctacggcc agcaacgatc caacgggaat caccggcagc gccagggcga acgcaatgcc
      601 ctgagcgacg ccgcaggcca cgcgcaagcc agtgaaccct cccggccctt gtccaaatgc
      661 gaccgccgtc aactgagagc gcgcgacgca cgcctgctgc agcaattgat cggccatggg
      721 cagtaatacc tcagcatgtt cgccggtggc gtcatgcgcc gaacatcttg ctgtcagacg
      781 tccctggacc agcgacaaca acgctacgct gcacgagcgc gatgacgttt caaaagcaag
      841 tagctgtaaa tccataatgc gcgcttttaa attactttac cggatcaact ttaccgcatt
      901 attatgtaac gcatgtggta acgtttcatg ccacgggcca aattttatcc gcacgccacc
      961 ccgcgtgcgc cccggcagtt cggcctatgg ttcaaacgcg cggcagcgtt attatgtaaa
     1021 ctgagttcta ccaaacgctt aatgtgtaat atcttgctac aacctctata cgcgttaacc
     1081 tgagcctgtt acattttcca ccatgaatac acaaaatcaa tccttatctc gcaaaatcct
     1141 tgtggtcgac gacgatccgc gattgcgcga cttattgcgc cgctacctga ccgaacaagg
     1201 ctttaatgtt tttgtcgccg aggacggtaa ggaaatgacc aagctctggc aacgagaaca
     1261 ttttgatttg ctggtgcttg acctcatgct tccgggcgaa gacgggctgt cgatctgccg
     1321 gcgtttgcgc ggcgggcacg acaacacgcc gatcatcatg cttacggcaa aagccgaaga
     1381 gatagaccgg atcgtgggtc ttgaaatggg ggccgacgac tatctgtcca agcctttcaa
     1441 cccacgcgaa ctgctggctc gggtaaatgc catcttgcgt cgccgcggca ccgaagagca
     1501 cccgggcgca cccagccagg aaaacgaatc tatcgaattc ggtccctatg ttcttaatct
     1561 gtccacgcgc acgcttacgc gcaacgacga acaagtcccc atcacgaccg gcgaattttc
     1621 ggttcttaag gtgttcgcgc gccatcccaa aataccgcta tcgcgcgaca agctgatgga
     1681 actggcccgc ggccgtgaat acgaggcctt cgaccgcagc ctggatgtgc aaatttcgcg
     1741 cctgcgcaaa ctggtcgaac ccaatccgtc aaaaccggtc ttcattcaga ccgtctgggg
     1801 actgggctat gtgttcgtcc ccgacggagg ccattgaaac ggcgaccgct gtgacacaat
     1861 aaggtgtttt ccatgtcgca gcccaaaaaa aggctcaaga aggccacctc acggctccgc
     1921 gttggccttt tcggccgctc cttccttctg cttgcagcgt taatgctggt cagtcttgga
     1981 gcctggctgc aggtgttctt cagtatggaa gaaggcccgc gcgccttgca gatggcgcag
     2041 cgtgtaacca cggcggtcag catcacgcgc tcggccatca tctacgcgcc tccgagcgta
     2101 cgcccctctc tgttgctgga tctcgccact aaagaaagcc tgcgcgtaca gccacgcgaa
     2161 gaacccgatg tgctgcaacc tctgccggat tccaattact gggatcgcgt ggcggccgaa
     2221 atcagcgagc gcctgggccc caaaacgcaa atcatgtgga cggtcaacca gacaccaggc
     2281 atctgggtca gctttgatat caacaacgat aaatattggc tcgtatttga ccgcgaacag
     2341 ttgggcctga cagcgggggc tgaatggttt ggctggggcg ccaccgcctt gctgctggcg
     2401 ctaataggcg ccgcagtcag cgtgcgtttc gtcaaccggc cactggctca actggccaag
     2461 gtggcgcaac aactggcgcg tggcgaaacc ccctccccgc tacccgaaaa agggccgctg
     2521 gaaatccgcg aaatgaatac tgcgttcaat cgaatggcgc gcgatcttca
//
LOCUS       DATCXU010000492         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_508298,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000492 DATCXU010000000
VERSION     DATCXU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508298"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIR04_11040"
     CDS             complement(<1..210)
                     /locus_tag="VIR04_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HEY9461322.1"
                     /translation="MRIIDVREVTKFIASPIRNAYIDFSKMTISLVAVLTDAVRDGQP
                     VVGYGFNSNGRYGQGGLIRERFAPRL"
     gene            464..1063
                     /locus_tag="VIR04_11045"
     CDS             464..1063
                     /locus_tag="VIR04_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY9461323.1"
                     /translation="MDKNVLAAMARWPDVPDVFGWLSLSPQGQWRLHPRGDAALSGPG
                     EPINSERILQFIDRNYACDRQGRWYFQNGPQRVFVRLDAAPYILRTTGSRRPLCMQTH
                     NGLNVRAISGWWLDESGRLYAQTEHGPGLIAGRDLMQVLESLHTPCDEPLTAVLENSP
                     KAEHAVPIKAEHAGVIYLNFCTDAAVSRELGFIGNPVAA"
     gene            1128..1715
                     /locus_tag="VIR04_11050"
     CDS             1128..1715
                     /locus_tag="VIR04_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY9461324.1"
                     /translation="MTAHAPSFYYPAPRTQQFCSQCAAPLTRLVLPDDNRKRDVCEKC
                     GAVHYQNPRNVVGVLGVFDKKILLCRRAIEPRYNKWTLPAGFMELGETTAQGALRETD
                     EEAGARVELGSLYTVINVPRVDQVHFFYLAKILTSELNPGPESIEAAFFDEADIPWPE
                     LSFRTVVTTLRHYLEDRKKGSFPVHHYDIPTVDVA"
     gene            1729..2481
                     /gene="aat"
                     /locus_tag="VIR04_11055"
     CDS             1729..2481
                     /gene="aat"
                     /locus_tag="VIR04_11055"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY9461325.1"
                     /translation="MNTLAWLDDESPLPSPDNALPDGLLAAGGRLSIKRLTEAYSMGV
                     FPWFNEGDPVLWWSPNPRMVLTCAEFKPSHSLQKKLRQLARREQYAGARIQIKMDTAF
                     DEVIKACAGPRTTQHGTWITPAVQAAYCAWHAAGHVHSVETWVDGQLMGGLYGVSLGR
                     FFFGESMFSHATDASKLALAYLVGFLIKNQVQHIDCQQQTSHLASMGAHPISRDCFLN
                     YLEQALQQPAPLWGKGQLFQSGVLASDGSKTP"
     gene            2512..3243
                     /locus_tag="VIR04_11060"
     CDS             2512..3243
                     /locus_tag="VIR04_11060"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY9461326.1"
                     /translation="MSHPTESTFKTLQFYSTAVYPCSYLPGRQARSQVAAPTHLIDAT
                     TYSGLVEQGFRRSGLFTYRPNCDACRACQPMRVDVFNFQPDRTQRKVWKRHRNLTTQV
                     MPLHWEAEHFELYKAYQSKRHPNAEADENSRSQYTQFLLTSRVESRLVEFRTPSGILQ
                     IVSIIDILETGLSSVYTFFDPDVPGSLGTYGILWQIEQCKNMDLPWLYLGYWIRESRK
                     MAYKSNFRPHQILTDEGWRQPVSPQ"
     gene            3264..>3397
                     /locus_tag="VIR04_11065"
     CDS             3264..>3397
                     /locus_tag="VIR04_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEY9461327.1"
                     /translation="MSFLFNSYGLARSALFSLDAETAHELTLSSLQKAYDCSLTRGLL
                     "
BASE COUNT          811 a          990 c          875 g          721 t
ORIGIN      
        1 caagcgcggc gcaaaacgtt cgcgaatcaa gccgccctgg ccataacgcc cgttcgaatt
       61 aaagccataa ccgactaccg gctgaccgtc gcgaaccgcg tcggtcaata cggcgaccag
      121 gctgattgtc atctttgaaa aatcgatata tgcattgcgt attggcgagg caataaattt
      181 ggtgacctct cgaacatcga taatgcgcac agccataacg tgtgtttcct taatatccgc
      241 agcagaaaaa aaatgaaagt aggcagctta tccgaaaact gacctcgaag gtagcggcgc
      301 cagccgctgc acaaaagccg ccagggcaag cacagtataa acatctctat aagccccata
      361 attaatatat attcacacta tcttggtgtg aaagttaaac aatgggtcaa ttcaaacgga
      421 tgtctgaaga ctttgccgtg tctcaaaatg ccaggttgaa cttatggata aaaacgtgct
      481 tgccgctatg gcgcgctggc ccgatgtacc ggatgtcttt ggttggctct cattgtcgcc
      541 gcaggggcaa tggcgcctgc atccgcgcgg agatgccgcc ctctccggtc ccggcgagcc
      601 gataaacagc gagcggatac tgcagtttat cgatcgtaac tatgcctgtg accgacaggg
      661 ccgctggtac tttcaaaatg gaccacagcg cgtgtttgtg cggctggacg ccgcacccta
      721 tatcttgcgc acgacgggat cgcgccggcc gttatgcatg caaacccata acggcctgaa
      781 tgtgcgcgcc atcagcggct ggtggctgga cgagtccggc cgcctttatg cgcagactga
      841 acacggcccc ggtcttattg ccgggcgcga tttaatgcaa gttctggaat cgcttcacac
      901 cccttgcgac gagcccttga ccgccgttct cgagaacagt ccaaaggccg aacacgccgt
      961 gccaataaaa gcggaacatg ctggcgttat ctacctgaat ttttgcaccg atgcagccgt
     1021 atcccgagag ctgggattca tcggcaaccc ggtcgccgca taacccgcgg ggctacccta
     1081 taatgaagtc tcctctccgt tgcggcccag ttcagcagaa ccttcgtatg acagcgcatg
     1141 ccccttcttt ttattaccca gccccccgaa cgcaacaatt ctgcagccaa tgcgccgcgc
     1201 cgctaacccg actggtgctg cccgacgaca accgcaaacg cgatgtctgc gaaaaatgtg
     1261 gggcggtgca ctaccaaaat cctcgcaacg tagtgggcgt gctgggtgtt tttgacaaaa
     1321 aaatcttgtt gtgccgccgg gctattgaac cacgctacaa caaatggacc ttgcctgccg
     1381 gcttcatgga actgggcgaa acgacagcac aaggagccct gcgggaaacc gacgaagaag
     1441 caggcgcccg agtggaactc ggctcgcttt atacggttat caatgtgcca cgtgtggacc
     1501 aggttcactt tttttatctg gctaaaatac tgacatccga actgaacccg ggacccgaaa
     1561 gcatcgaagc agcctttttc gacgaggccg atattccgtg gccggaacta tcctttcgca
     1621 cggtcgtcac aactctgcgc cattatctgg aagacaggaa aaagggaagc tttcctgtcc
     1681 atcactacga tattcccacc gtcgacgtgg cgtaggtccc cgcaacacgt gaacaccctg
     1741 gcctggctgg acgacgaaag tccgcttcca tctcccgata acgccctgcc ggacggcttg
     1801 ttggccgccg gaggaaggct gtccatcaag cgacttaccg aggcctattc catgggcgta
     1861 ttcccatggt tcaacgaagg cgacccggtg ctatggtgga gtcccaatcc gcggatggtg
     1921 ctgacctgcg ccgaattcaa gccgtcacat tcattgcaga aaaagctgcg tcagctcgct
     1981 cgccgggagc aatacgcggg cgcgcggatt caaataaaaa tggacaccgc gttcgatgaa
     2041 gttattaaag cgtgcgccgg gcccagaacg actcaacacg gcacctggat tacgccagcc
     2101 gttcaggcgg cctattgcgc ctggcacgcg gctgggcatg tacatagcgt ggaaacctgg
     2161 gtggatgggc aactgatggg cgggctttac ggcgtcagcc tggggcgatt tttctttggc
     2221 gaatccatgt ttagccacgc cacggacgcc tcaaagctgg cgctggctta cctggtcggt
     2281 tttttgatca agaaccaggt gcaacatatc gactgtcaac aacaaacaag ccatttagcc
     2341 agcatgggcg cgcaccccat cagtcgggac tgcttcttga attatctcga acaagccttg
     2401 cagcaacccg cgccactctg gggcaaagga cagctctttc aatcgggcgt gcttgcatcg
     2461 gatggatcaa aaacgccata atgcctgtca aagcatcaac gagtttttgc catgagccac
     2521 cccacggaat cgacatttaa aacgttgcag ttttactcca ccgcggtcta cccatgcagt
     2581 taccttccgg ggcgtcaggc gcggtcgcaa gtggccgcgc caactcacct tatcgacgcg
     2641 acgacctact ccggcctggt tgagcagggt ttccgccgca gcggcttgtt tacgtatcgt
     2701 ccgaattgcg acgcgtgccg cgcctgtcag cccatgcgcg tagacgtatt taattttcaa
     2761 cccgacagaa cccagcgcaa ggtatggaag cgccacagaa acctgacaac tcaagtcatg
     2821 ccgctacact gggaggccga gcatttcgag ttgtacaagg cctatcaatc aaaacgccat
     2881 cccaacgcgg aagcggacga aaacagccgg tctcagtaca cgcaattttt attaaccagc
     2941 cgggtagaat ccagactggt cgagtttcga actccttcgg gaattctcca gattgtctcc
     3001 atcatcgaca tccttgaaac cggcctgtca tcggtctata cattcttcga tcccgacgta
     3061 cctggcagct tgggaactta cggaattcta tggcagatag aacaatgcaa aaacatggat
     3121 ttaccatggt tatacttggg ctactggatc agggaaagcc gcaaaatggc gtataaatcg
     3181 aactttcggc ctcaccagat tctgaccgat gaaggctgga ggcaacccgt cagcccgcaa
     3241 tagcaacagg ccctcgccac actatgtcat ttctttttaa ttcctacggc cttgcccggt
     3301 ctgcgctctt ttcgctggac gcggagaccg cccacgaact caccttaagc tcgttgcaaa
     3361 aagcgtacga ctgctcgctg acccgcgggc tactgca
//
LOCUS       DATCXU010000493         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_996563,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000493 DATCXU010000000
VERSION     DATCXU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996563"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VIR04_11070"
     CDS             complement(<1..196)
                     /locus_tag="VIR04_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY9461328.1"
                     /translation="MSLAVLASRALCGLAALPVRVEVHVGTGLPAFHVVGLPDAGVRE
                     SRERVRSAIICSGFEFPAGRI"
     gene            402..614
                     /locus_tag="VIR04_11075"
     CDS             402..614
                     /locus_tag="VIR04_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461329.1"
                     /translation="MSTSSYAEASLLTKTPDHTAIHAVISGAMSVLKAAYATARRILT
                     AFLDFAVEVQELQDATFKTLPYIAHK"
     gene            complement(714..983)
                     /locus_tag="VIR04_11080"
     CDS             complement(714..983)
                     /locus_tag="VIR04_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY9461330.1"
                     /translation="MVNRTDWFEDFRKNMSDLIAKTPAADIERTVKAMATQTFSKMDL
                     ITREEFDVQADLLERAIARIAELEIKVRDLESGTGVATERQEPTQ"
     gene            complement(1075..1497)
                     /locus_tag="VIR04_11085"
     CDS             complement(1075..1497)
                     /locus_tag="VIR04_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461331.1"
                     /translation="MQKNRTHFAVIALVASMSLLAACSKGEDKNANKSPAASEAPMPA
                     PAPVAPPAAPAPAAPAPATPSAAPSSGSMDNAKESVKNAADKIGDKAAELKDSAVDKA
                     HEMGNAIREGAAKADQKIQDTVGNGSGSNSPATPPAAR"
     gene            2032..2370
                     /locus_tag="VIR04_11090"
     CDS             2032..2370
                     /locus_tag="VIR04_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY9461332.1"
                     /translation="MKLITAIIKPFKLDEVREALSELGIQGMTVTEVKGFGRQKGHTE
                     LYRGAEYSVDFLPKLRVEMAIPEHMLEQAIEKLLAAAYTGKIGDGKIFVSPIEQAIRI
                     RTGEFGEDAL"
     gene            2385..3611
                     /locus_tag="VIR04_11095"
     CDS             2385..3611
                     /locus_tag="VIR04_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY9461333.1"
                     /translation="MDKADLVWVSFSTLLVLMMAMPGLAMFYGGLVRGKNVLSVMIQV
                     LSTFVIGIIIWVLYGYSLALTEGNAVIGGGSRLLLHGVVNLGTQSFSPSATIPELTFI
                     AFQATFAAITCALIVGAIAERANYAAVLLFTVIWLSLAYLPIAHMVWAPDGWLLKQGA
                     LDFAGGTVVHINAGIAGLTSAYYVGRRIGYGRESMQPHNLPMTMIGAALLWVGWFGFN
                     AGSALGANTTAALAFFNTLLATAGAVIAWVAVEWKVKGRPSLLGAASGAVAGLVGITP
                     AAGFVGLNGALIIGLATGAICVWGVNGLKRLLRADDTLDVFGIHGVGGITGALLTGVF
                     NAQALGGPGLKTIGQIPHQLWLQSEGILITLVWSGLVAWLACFVADKVCGLRVSADAE
                     REGLDIHLHGETAYQR"
BASE COUNT          788 a          995 c         1012 g          886 t
ORIGIN      
        1 tgatccttcc tgccgggaat tcaaagccgc tgcaaataat ggcagaacgc acgcgttcgc
       61 ggctttcgcg cacccccgcg tccggcagcc caacgacatg gaacgccggc aagcccgtgc
      121 cgacgtgcac ttcgacgcga actggtaaag cggccaggcc gcaaagcgcc cggcttgcca
      181 acaccgccaa tgacatatgc ccccctacca ccgctctctt tactgcaaga gcttgtcaag
      241 gaacaagtat gaaggcatta acggcccgcc gggacgtggc gtgccagctt tggccattga
      301 gctttatacg caagggacag tagtgattag ggtaaaccct tgatcgaaaa tagggtatac
      361 cctataatga gtcatcttaa acagtcataa ccaaggacat catgtcgacc agttcatacg
      421 cagaagcgtc ccttcttacc aagacgccgg accacactgc aattcatgcc gtcataagcg
      481 gcgcaatgtc agtcctgaaa gccgcctatg ccactgcaag gcgaattctg accgccttcc
      541 tggatttcgc cgttgaagta caggaactgc aggatgccac tttcaagact ttgccttata
      601 tcgcccataa gtaacaccct tgttcaaggc accataagca gaacccgctg tgcgcagcgg
      661 gtttttttat ggtgtccggg gaaatgtgcg acattttgcg gcgggcgcaa aaactattga
      721 gtgggctctt gtcgctcggt agcaacaccc gtacctgatt ccaaatcccg cactttaatt
      781 tcaagctccg ctatgcgcgc aattgcccgt tccagcaaat cagcctgaac gtcgaactct
      841 tcgcgcgtga tcagatccat cttggaaaat gtttgcgtcg ccattgcttt tacggtgcgt
      901 tcaatatcgg ccgcaggggt tttcgcaatc aaatccgaca tgttcttgcg aaaatcctcg
      961 aaccagtctg tgcgattgac catatctgat ctccgtaaaa attattaatg gaatagccta
     1021 gtgtaatgaa aaacgggccg ttaaagcccg ttttttgcaa gaatgagttg tgggttacct
     1081 ggcggcgggc ggggtggcag gcgagttcga acctgaacca ttgccaaccg tgtcctgaat
     1141 cttctggtcg gccttggcgg ccccttccct gatcgcattg cccatttcat gagctttgtc
     1201 aacggcgcta tctttaagtt cggctgcctt gtcgcctatt ttatcggcgg cgttcttgac
     1261 agattccttg gcgttatcca tcgaaccact cgagggcgct gccgagggcg tggccggcgc
     1321 aggcgccgcc ggggctggag ctgctggtgg agcgaccggg gccggcgctg gcattggcgc
     1381 ctcagatgcc gccgggctct tgtttgcatt tttgtcttcg cctttggagc acgccgccaa
     1441 taggctcatc gaggccacca gagcgatgac ggcaaaatgt gtgcgatttt tctgcataat
     1501 tcttttttcc ttgtccaacg tcgcgtaaca ccgtttagat attattcact gacgccgctg
     1561 cgacggacgg ctcagtagcg tgctgattgc aaccgcttgg aaacaattct agtgatcaaa
     1621 gattgggtac ggccaaacct agtgtaaatc ctaatttcaa aaataaaatt ttctagttct
     1681 attttttttc aacccggcct tgcgtcgcac tgttgttggc gaatttcctg gcttttttgt
     1741 ctatacacca ggaaacaaaa ctgtcgcaga ttcaactttt tgttacacaa ccctatgcgg
     1801 caaacagcag aaaagcaaca tcagacagga ctggctgtgt atccagatcg atccagtgtg
     1861 gtgcgcgtac acacgcccgc gcaccactat gatgctgcgt tgcccgctca ctagccggat
     1921 aagttgccaa aatctggcat ggaatttgcg tatgttcctg ttgcgcgccg tccatcggtg
     1981 tggtgggccg ccctttccgg gctcgggcgc cgatatctct ctggagtccc aatgaaatta
     2041 attacagcca tcatcaaacc gttcaagctc gatgaagtcc gcgaagcctt gtcggagctt
     2101 ggcattcagg gcatgactgt caccgaagta aagggattcg gccgtcaaaa agggcatacg
     2161 gaactatacc ggggcgccga atacagcgtc gactttctgc cgaagctgcg tgtggagatg
     2221 gccataccgg aacacatgct ggaacaagca attgaaaagc tgcttgcggc agcctatacg
     2281 ggaaaaatcg gcgacggaaa aatttttgta tcaccaatcg aacaggcaat tcgaatccgc
     2341 accggtgaat ttggcgaaga cgcactataa caaggtgaaa taaaatggat aaagcagatt
     2401 tggtatgggt gagtttttca accttattgg tcttgatgat ggccatgcct ggcctggcca
     2461 tgttttacgg cggcctggtg cgcggcaaaa acgtcttgtc ggtaatgatc caggtgcttt
     2521 ccacctttgt gatcgggatc attatctggg ttttgtacgg ctattccctg gcccttaccg
     2581 aaggcaatgc agtgatcggc ggaggatcaa ggttgcttct gcatggcgtg gtcaatctgg
     2641 gcacacaaag tttctcgccg tccgccacga tccccgaact tactttcatt gcctttcagg
     2701 caacctttgc cgccattaca tgcgcgctga ttgtcggagc gatcgccgaa cgcgccaact
     2761 atgccgccgt gctgctgttt accgttatct ggctcagtct tgcctattta ccgattgcac
     2821 acatggtttg ggcgcccgac gggtggcttt tgaagcaagg cgccctggat tttgccggcg
     2881 gcacagtcgt ccatatcaat gccggcatcg ccggtctgac gagcgcctat tacgtcggac
     2941 ggcgaatcgg gtacggccgc gaatcaatgc agccgcataa tcttcccatg acaatgatcg
     3001 gcgcagcctt gctgtgggtc ggctggttcg gctttaacgc gggctcggcg ctgggcgcca
     3061 acaccacggc ggcactcgca ttctttaata cgctgctggc tactgcgggc gcggtaatcg
     3121 catgggtcgc agtcgagtgg aaggttaaag gtcggccctc gctattgggc gccgcatcgg
     3181 gcgcggtcgc gggactggtg ggaattactc cggccgcggg ttttgtcggg ctcaacggtg
     3241 cgctgattat tgggctggcg acgggggcta tctgcgtgtg gggagtcaac ggccttaaac
     3301 gcctgttgcg cgccgatgac acgctggacg tatttggcat acacggcgtg ggaggcatta
     3361 ccggggcgct gttgacgggc gtattcaacg cccaggccct gggcgggccc ggcctcaaaa
     3421 ccatcggaca aatacctcac cagctctggc tgcaatccga aggcatttta attacccttg
     3481 tatggtccgg actggttgcg tggctggctt gttttgtagc ggacaaggta tgcggcttgc
     3541 gcgtcagcgc cgacgcggaa cgtgaagggc tggatattca tttacacggg gagaccgcgt
     3601 accagcgttg atttacttta actggtcagg gccgctaaat cgatgcgttg ggcgcgattt
     3661 agcgccgcgg ccacctttac g
//
LOCUS       DATCXU010000494         5133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_686061,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000494 DATCXU010000000
VERSION     DATCXU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686061"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VIR04_11100"
     CDS             complement(<1..946)
                     /locus_tag="VIR04_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY9461334.1"
                     /translation="MSQILKQPIKLIAMQGDGIGPEITQAALDVLRAAQTAFDLNIQV
                     EFAEIGFASLKKTGTTISDDVVARARAADGVILGPISHNEYPSLAEGGRNPSGVLRKE
                     LDLFANVRPARTRAGVESPIRKNFDLVIVRENTEGFYADRTMVAGTGEFMPTEDVALA
                     VRKITAKGCQRIAEYAFKLALQRGAKKVTAVHKANVLRLSDGFFLEKTRAVAAKFPTI
                     AYDEQLVDSMTALLIRKPEIFDVVVTTNMFGDIISDEASELSGGLGLAASLNAGFDHA
                     IAQAQHGSAPDIAGQDKANPSSLIGSVAMLLRWLSERRG"
     gene            complement(999..2033)
                     /locus_tag="VIR04_11105"
     CDS             complement(999..2033)
                     /locus_tag="VIR04_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY9461335.1"
                     /translation="MAKKYTKIDVASLKRLAVDAFCRAGLSAADAATAAHILVEADMM
                     GTATHGVLRLQSYTDRLRSAGINGNPRIVVDKRAPSLALVDGDNGLGPVVGGKALNVA
                     IEMVRETGMAYVGCRNSNHFGALAPYAIQACDAGFVFMGGTNASTTICPWGGREARLG
                     NNPFSIAAPCRDGFHFILDMAMSVAARGKIRAARDNGEPIPAGWAVDKNGLPTTNPLE
                     ALSGFLLPMGGYKGSGLSMAVDILAGVLTGGQFLNTISSWVDNPDAPSRVGHFFVLID
                     PDRLIGRENFALAMDRFRRIVLDTPPAVESTPVVLPGQIEQTRRQKALAEGIEIPSDL
                     LASIRLLAAG"
     gene            2132..3568
                     /locus_tag="VIR04_11110"
     CDS             2132..3568
                     /locus_tag="VIR04_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY9461336.1"
                     /translation="MRQTLPVATAADAQTARSETQPLTMRVAEKICHFQRPMATPFAM
                     RQACTAMTDTIAVALAGAPESCTQILLNTPGIATAPGKSLVFATKQRTSALDATLVNG
                     TASHALDYDDFSGVMGGHQSAPIVPALFALGEEHRSSGEKIVLAYLVGVETELRFSRA
                     VNMHHYDIGWHPTSTLGVFGAAAATSYLLGLDIPHTAMALAIAASLANGLKANFGTMT
                     KPLHIGQCGRNGLLATLLAAGGFDANPAVLEHQQGFLNVFNGPGLYDSERLLSNWGRP
                     LEVEDPTIGLKQFPCCGSTHPAIMMALQLVQEESVHAEDVVNITIMPHGRRLKHTDNP
                     HPASSLEAKFSVQYVVARALVDGDVRLKDFEGNAYNEPAVRRLLAITTAQAHPDMADD
                     ADGQWGAEVIVALKDGRKLSRRVDNLVGRGGDNPMTRAELWRKFSNCAEKSLAGRQIE
                     PLFEQLQQLDQIPDINQITGLMLGAERA"
     gene            3611..4405
                     /locus_tag="VIR04_11115"
     CDS             3611..4405
                     /locus_tag="VIR04_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY9461337.1"
                     /translation="MDITSPAKIKVFWQPGCSSCLRTKEFLASQGIDFEAIDVHNDPD
                     GMAQLIALGARSVPVVAVGNRYTFCQSMRDVVKFLGLKTKLKDALPAEQLVAKLDLVM
                     TAAARYLRQLTEEQSREYFRDRKRSPGGLAFHVFRVAELGMQTAQGIELRPDGFKDVP
                     PPDWTREDIARWGEDVQQRVRTWWQNEPDKTLEYDVTTYYGRRPMHEVFERTAWHAAQ
                     HTRQIMLILETLGITPAQPLTASDLAGLPLPNEVWDIQNKGFNEPG"
     gene            4392..>5133
                     /locus_tag="VIR04_11120"
     CDS             4392..>5133
                     /locus_tag="VIR04_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY9461338.1"
                     /translation="MSQDNDICFMTATEMAASIRAGDLSAREIIEAHLARIDSINPKV
                     NAIVTLHAEQALIAAKAADEAQVHGATLGPLHGLPVAHKDLLLTKGMRTTFGSKIHEH
                     FVPAENSLIVERQQQAGAISLGKTNTPEFGAGSQTFNTVFGVTRNPYDLSKTCGGSSG
                     GSAVALATGMVALSDGTDMFGSLRCPANYTNIVGLRPSIGRVPQIPELDGWDSLSVSG
                     PMARTVKDIALHLSVMAGPDARDPLSLSE"
BASE COUNT         1182 a         1541 c         1373 g         1037 t
ORIGIN      
        1 ggccgcgccg ctccgacaac caccttaaga gcattgccac cgaccctatc agtgatgatg
       61 gattcgcttt atcctgaccg gcaatgtccg gagccgaacc gtgctgggcc tgtgcgatgg
      121 catgatcaaa accggcattc agagacgccg ccagccccag gccgcccgaa agttccgagg
      181 cctcgtccga aatgatgtct ccgaacatat tcgtggtcac aaccacatcg aaaatctcgg
      241 gtttgcgaat cagtagcgca gtcattgaat ccacaagctg ctcgtcgtaa gcaatggttg
      301 gaaatttcgc ggcgacagca cgcgtttttt caaggaaaaa cccatccgac aatcgcaata
      361 cattagcttt atgcaccgcc gtcacttttt tggcgccgcg ctgcagtgcc agcttgaatg
      421 catactcggc aatacgctgg caccctttgg cggttatctt tcgcaccgcc agcgcgacat
      481 cctcggtagg cataaattca cctgtaccgg ccaccatggt ccggtcggca taaaagcctt
      541 ccgtgttttc acgcacaatc accagatcaa aatttttgcg aatggggctt tcgacacctg
      601 cgcgcgtgcg cgcgggtcgc acgttggcaa acaaatccag ttctttgcgc agcactcccg
      661 acggattacg tccgccttcg gcgagagacg ggtattcgtt gtgtgaaatg gggccaagaa
      721 tcactccgtc ggcggcgcga gcccgcgcca ccacgtcatc gcttatcgta gttcccgttt
      781 ttttcagaga cgcgaaccca atctcggcga attcgacctg tatattgagg tcaaacgcag
      841 tctgcgccgc gcgaagcaca tcaagggccg cctgtgttat ctctggccca atgccatcgc
      901 cctgcatcgc gataagcttt atcggttgct taagtatctg agacacgtca atttcctatt
      961 tttatttcac gccaagaaat tagagacaaa acattccatc agcccgctgc aagcaatctt
     1021 atcgatgcga gaaggtcact gggtatttca atgccttccg ccaaggcttt ctggcgacgt
     1081 gtctgctcaa tttgccccgg taaaaccacc ggcgtcgatt caacggccgg cggcgtatca
     1141 agcacaatcc gccggaagcg atccatggcc aaagcaaaat tttcacgccc gatcaatctg
     1201 tcggggtcga tcaatacgaa aaaatgcccc acccgagagg gagcgtcggg gttatctacc
     1261 caggacgaaa tggtattcag aaactggccg cctgtcagta cacccgccag aatgtcaacc
     1321 gccatcgaaa gaccggaacc cttatagccg cccattggca acagaaaccc cgatagcgct
     1381 tcaagcgggt tcgtcgtggg caggccgttt ttatcgacag cccagcccgc gggtatgggt
     1441 tcgccgttat ccctggcggc tcttatcttg ccgcgcgcgg caacgctcat tgccatatcg
     1501 agaataaaat gaaagccatc ccggcatggg gcggcgatgc tgaacggatt gtttcccagt
     1561 cgcgcttccc tgcctcccca agggcatatc gtcgttgacg cgttggtgcc gcccataaat
     1621 acaaagccgg cgtcgcacgc ctgaatggcg tacggcgcca aggcgccaaa gtggttgcta
     1681 ttgcggcatc ccacgtaagc cattccggtt tcgcgcacca tctcgatcgc cacgttcagg
     1741 gccttgccgc caaccaccgg ccccaaccca ttatcgccat ccaccagagc aagcgaaggg
     1801 gcgcgtttat ccacgacaat tcggggattg ccattgatgc ccgcgctacg caggcgatct
     1861 gtataagatt gcaggcgcaa taccccgtga gtggccgttc ccatcatgtc ggcctcgacc
     1921 aggatatggg cggccgtggc agcgtccgca gccgaaaggc cggcccggca aaatgcatcg
     1981 acagcaagtc ttttcaacga cgcgacatca atttttgtgt attttttcgc catccgcttc
     2041 tcgttgcttt tatcagtctc tgcaataata aactacgcgt atacttctgt atgcaagtag
     2101 tatacgtcgc aatttggatc cggagaaaca aatgcgtcaa actcttccag tagctactgc
     2161 ggcggatgcg cagaccgccc ggtccgaaac ccagccgctc accatgcgcg tggcggaaaa
     2221 aatatgccat ttccaacggc caatggccac tcccttcgcg atgcggcaag cttgcacggc
     2281 aatgaccgat accatcgcag tggcattggc cggcgcgccg gaaagctgca cgcaaattct
     2341 gctgaacacg cctggaatcg ccaccgcgcc gggaaaatcc ctggtttttg ccacaaaaca
     2401 gcgcaccagc gctctcgacg ccacgttggt aaatggaacc gcgtcacacg ccctggacta
     2461 tgacgacttc agcggcgtta tgggagggca ccagtcggcg ccgatcgttc cggcgctttt
     2521 cgcgttaggc gaagaacacc ggtcatcagg cgaaaaaatt gtgctcgcct atctggtcgg
     2581 cgttgaaacc gaattgagat tcagccgcgc cgtaaatatg catcattacg atataggctg
     2641 gcaccccact tcaacgctgg gagtctttgg cgcggcggcg gccacgtcct atctgcttgg
     2701 cctggatatt ccgcacacgg ccatggcgct ggcgattgcc gcatcgctgg ccaacggcct
     2761 gaaagccaat ttcgggacca tgaccaagcc tttgcacatc ggtcagtgtg gcagaaacgg
     2821 gctgctggcc accttgctgg ctgcgggcgg cttcgatgcc aaccccgccg tgctggaaca
     2881 tcagcaggga tttttaaatg tcttcaatgg gcccggactg tatgattccg agcggctatt
     2941 aagcaattgg ggacgccccc ttgaagtcga agacccaacc atcggactaa aacaattccc
     3001 ctgttgcggc agcacccatc cagccatcat gatggcgctg caacttgttc aggaagaaag
     3061 cgttcacgct gaagacgtcg tcaacatcac gatcatgccg cacgggcggc ggctgaaaca
     3121 tacggacaat cctcacccgg ccagctcgct tgaagcaaaa ttcagcgtgc aatacgtcgt
     3181 ggcgcgggcg cttgttgatg gcgacgtccg cctgaaagat ttcgaaggca atgcttacaa
     3241 cgaacctgcc gtccgtcggc ttttggccat caccaccgcg caagcgcacc ccgacatggc
     3301 cgacgacgcc gacggacaat ggggcgccga ggtcatcgtc gcgctcaaag acggccgcaa
     3361 actgtccaga cgcgtggaca acctcgtggg gcgaggcggc gataacccga tgacacgtgc
     3421 cgagctctgg agaaaattca gtaattgcgc cgagaagtcg ctggctggcc ggcaaatcga
     3481 acccttgttc gagcaactcc agcaactgga tcaaataccg gacataaacc agattacggg
     3541 gcttatgcta ggcgcggagc gagcctgaat gtatactttc caatgctgat tactgccagg
     3601 agaatgttcc atggacatta catcacctgc aaagattaaa gtgttttggc agcccgggtg
     3661 ctcatcctgc ctgcgcacaa aagagttcct tgccagtcag ggcatcgact tcgaagccat
     3721 tgacgtccat aacgatcccg acggcatggc ccagctcatt gcgctgggag cgcgcagcgt
     3781 acctgttgtt gctgtaggca accgttatac gttttgtcag tccatgaggg atgtcgtcaa
     3841 atttctaggg ctaaaaacca aacttaagga cgcgttgccc gccgaacagc tcgtcgcaaa
     3901 actggacctt gtaatgaccg ccgcagcacg gtatttgcgt caacttaccg aggaacagtc
     3961 gcgcgaatac ttccgtgatc gcaagcgcag ccccggaggg ttggcattcc acgtatttcg
     4021 cgttgccgaa ctcggaatgc agacagcgca aggcattgag ttgcgaccgg atggatttaa
     4081 agatgtgccg ccccccgact ggacccgcga agatatcgcc cgctgggggg aggacgtgca
     4141 gcaacgggtt cgaacatggt ggcaaaacga gcctgataaa acattagaat acgatgtgac
     4201 cacatattat ggccggcgcc caatgcacga agtattcgag cgcaccgcct ggcatgcggc
     4261 gcagcacaca cggcaaatca tgctgattct ggaaacgctt ggaatcacgc ctgcacagcc
     4321 cctgacggcc agcgaccttg ccgggttgcc gttgcccaat gaagtgtggg acatacaaaa
     4381 caagggtttc aatgagccag gataacgata tttgtttcat gacagccacc gagatggctg
     4441 cgtccatccg ggccggcgac ttgtcggcgc gcgaaatcat cgaggcccat ctggcccgca
     4501 tcgatagcat caaccccaag gtcaatgcga tcgtcacgtt acacgccgaa caagcgctga
     4561 tcgcggccaa ggccgccgac gaggctcaag tacacggagc aaccctgggt ccactgcacg
     4621 gtttgccggt tgcccacaaa gacctgctgc ttaccaaggg aatgcgcaca accttcggct
     4681 ccaaaattca cgaacatttc gtcccagccg aaaactcgct catcgtcgaa cgccagcagc
     4741 aagcgggagc catcagcttg gggaaaacca atacgcctga attcggcgcc ggctcgcaaa
     4801 cattcaatac ggtattcggt gttacacgca atccctacga cttatccaaa acctgcggcg
     4861 gctcaagcgg gggttcggct gtcgccctgg cgacaggcat ggttgcgcta tcggatggca
     4921 ccgacatgtt cgggtcgctg cgctgcccgg caaattacac caacatcgtg gggctgagac
     4981 cctcgatcgg ccgtgttccg caaatcccgg agctggacgg ctgggacagc ttgtctgtca
     5041 gcggaccaat ggcgcgcacg gtcaaagata tcgcgctgca tttatcagta atggctggcc
     5101 ccgacgcacg cgacccgctg tccctgagcg aag
//
LOCUS       DATCXU010000495         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_93998,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000495 DATCXU010000000
VERSION     DATCXU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93998"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VIR04_11125"
     CDS             <1..423
                     /locus_tag="VIR04_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY9461339.1"
                     /translation="VLCLQPPLHQVQTRVQACAEFAHLPEAESLAVANKRISNLLKKA
                     PEKLQHVNRGALTEPAEQELARVLEKLKPQAQAQFVRGDFTASLATMAQARKAVDNFF
                     NDVMVMADDPAIRSNRLALLADLHQVMNQVADISRLAQ"
     gene            420..956
                     /gene="gmhB"
                     /locus_tag="VIR04_11130"
     CDS             420..956
                     /gene="gmhB"
                     /locus_tag="VIR04_11130"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY9461340.1"
                     /translation="MKLAILDRDGVINRDSDSFVKEPDEWIALPGSLEAIARLSRANW
                     HVVIASNQSGVARGLFSMATLNAIHAKMRRELAHAGGNIEAIFICPHGPDEGCPCRKP
                     ETGLFQDIARRYDIDLSGSPAVGDSLRDLQAASAAGCEPWLVLTGKGAQTEKAGHLPA
                     GTRVRENLAAVVDAWLKD"
     gene            960..1697
                     /locus_tag="VIR04_11135"
     CDS             960..1697
                     /locus_tag="VIR04_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY9461341.1"
                     /translation="MAVLRATLYMAFLIVTVIPYALACVLWSPLPSRWRYRLTVGWPT
                     LALWGARFILGIRWQVKGAENLPDGPAILLCKHQSAWETLFIPAYMPREVCFVYKKEL
                     HRVPFFGWGLALLHMIPIDRSRGRDAFEQVVKLGQQRINEGRWPILFPEGTRIAPGKT
                     GRYKAGGALLAVRTNTGVIPIAHNAGECWPRNAFVKKPGLVTVSIGPMIDSKGLDAET
                     LNQKVRNWIENEMRQLNPERYESDQAD"
     gene            1675..2439
                     /locus_tag="VIR04_11140"
     CDS             1675..2439
                     /locus_tag="VIR04_11140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY9461342.1"
                     /translation="MNPTKPTNERWREVQADQHTIGFTLRRSRRKSIGLVINGDGLQV
                     TAPNWVTLREIDEAVVERSRWILAKLTLNQARQARLAAINTHWRDGGDIPYLGQNIIL
                     KLDGSRNHAVFDGSIFAPRQDDTLCLALPDDAECNHVRDSANMWLQQQAKIWFEQRLQ
                     HFLTVGQLQIKRWRLTSAATRWGSCSSDGSIRLNWRLIHFEHAIIDYVVAHELAHLRE
                     MNHSKNFWREVGRIFPDFEPARAALRQHDPASLPLV"
     gene            2470..2865
                     /gene="gloA"
                     /locus_tag="VIR04_11145"
     CDS             2470..2865
                     /gene="gloA"
                     /locus_tag="VIR04_11145"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY9461343.1"
                     /translation="MRILHTMLRVGDLDRSILFYTDVLGMKLLRRKDYPEGKFTLAFV
                     GYQDESEGPAIELTHNWETASYELGNGYGHVALEVDNAYDACAKIKEKGGNVTREAGP
                     MKHGQTVIAFVEDPDGYKIELVQHKPQTV"
BASE COUNT          650 a          834 c          846 g          575 t
ORIGIN      
        1 gtgctgtgtc tgcaaccgcc gctgcaccag gttcaaacca gggtgcaagc ctgcgccgaa
       61 ttcgcccatc tgcccgaggc cgaaagcctg gctgtggcca acaagcgcat ttccaatctg
      121 cttaaaaaag cgcctgaaaa acttcagcat gttaaccgcg gcgcgctcac cgaacccgcc
      181 gaacaagaac tggcgcgggt cctcgaaaaa ttaaagccgc aggcgcaggc gcaattcgtt
      241 cgcggcgact ttaccgccag cctggccacc atggcgcagg ccagaaaggc cgtggataat
      301 ttctttaatg acgtcatggt catggccgac gatcccgcca tacgctccaa ccggctggcc
      361 ctgctggcgg atttgcatca agtaatgaac caggtcgccg atatttcaag gttggcccag
      421 tgaagcttgc catactcgat cgcgatggcg tcatcaatcg ggacagcgac tcttttgtaa
      481 aagagcctga tgaatggatc gcattgccgg gcagcctgga agcgatagcg cgcctttcgc
      541 gcgcaaactg gcatgtcgtc atcgccagca atcaatcggg tgttgctcgg ggcttgttca
      601 gcatggcaac cttaaacgcc attcatgcca aaatgcggcg tgaactggct catgccggcg
      661 gcaatatcga agccatcttt atctgtccgc acggtcccga tgagggatgc ccctgccgca
      721 aacccgaaac cggcctgttc caggatattg cccgccgtta cgatattgat ttaagcggaa
      781 gcccggcagt gggcgattcg ttgcgggatt tgcaagccgc cagcgcggcg ggttgcgaac
      841 cctggctggt tctgacgggc aaaggggcgc aaaccgagaa ggccggccac ttgcccgccg
      901 gcacacgcgt cagggaaaac ctggctgccg tcgtcgacgc gtggctcaag gattagaaca
      961 tggccgtgct gcgcgcaacg ctgtacatgg cgtttttaat cgttactgtc atcccctacg
     1021 cgctggcctg tgttctgtgg tcgccattgc catcgcgttg gcgctataga ttgacggtgg
     1081 gctggccgac gctggctctt tggggcgcgc gattcatact tggcatacgc tggcaggtaa
     1141 aaggcgccga aaaccttccg gatggccccg ctatcctgct gtgcaaacat cagtcggcat
     1201 gggagactct atttattcca gcctatatgc cgcgcgaggt ctgtttcgtg tataaaaagg
     1261 aattgcatcg ggtgccgttc tttggctggg gtctggcttt gttgcatatg attccgattg
     1321 atcgctcccg cggccgggac gcattcgaac aagtggtcaa gctgggacaa caacgcatca
     1381 atgaaggacg gtggcctatt ctgtttcccg agggcacgcg cattgcccct ggaaaaacgg
     1441 ggcgctacaa ggccggcggc gcccttctgg ccgtgcgcac caacacgggg gtcattccca
     1501 tcgcccacaa tgcgggcgaa tgctggccgc gcaacgcttt cgtaaaaaag cctgggctgg
     1561 tcaccgtatc gatcggcccg atgatcgaca gcaaaggcct tgatgccgaa accctgaacc
     1621 agaaggtgcg gaactggatt gaaaacgaaa tgcgccaact gaacccggaa cgctatgaat
     1681 ccgaccaagc cgactaatga acgctggcgt gaagtgcagg ccgatcagca tacgatcggt
     1741 tttaccttgc ggcgttcccg gcgcaaaagc attggtctgg ttatcaatgg cgacggtttg
     1801 caggtaacgg cgccgaattg ggtgacgctg cgcgaaatcg acgaggccgt ggttgaacga
     1861 tcgcgctgga ttctggccaa gctaacgctt aatcaggcgc gccaggcccg gctcgcggcg
     1921 atcaataccc actggcgcga cggcggcgac atcccttacc tgggacagaa tattattctg
     1981 aaactggacg gctcgcgaaa ccacgccgtt ttcgatggct caatattcgc tccgcggcag
     2041 gatgacacgc tttgcctggc attgcccgac gacgccgaat gcaaccacgt gcgcgacagc
     2101 gcgaatatgt ggctgcagca acaggcgaaa atttggttcg agcaacgctt gcagcatttt
     2161 ctaactgtcg gccagcttca aataaagcga tggcggctta cgtcggcggc caccagatgg
     2221 ggttcatgca gcagcgatgg aagcattcgg ctgaattggc gcctgatcca tttcgaacac
     2281 gccattatcg attacgtggt ggcccacgag ctggcccacc tgcgcgagat gaatcacagt
     2341 aaaaattttt ggcgtgaagt cggccggatc tttcccgatt tcgaaccggc gcgcgccgcg
     2401 ttgcgccagc atgacccagc ctcgctgccg ctggtttaaa cttctggggt actcagaaag
     2461 gagactatga tgcgaatact gcacaccatg ttgcgtgtcg gtgatctgga ccgatccatt
     2521 ttgttctaca ccgacgttct cggcatgaaa ttgctgcgtc gcaaagacta ccccgaggga
     2581 aagttcacac tggccttcgt gggctaccag gatgaaagcg aaggccccgc catcgaattg
     2641 acgcacaact gggagacggc gagctacgag ctgggcaatg gttacggcca tgtcgcactg
     2701 gaagtggaca acgcgtacga cgcgtgcgcg aagatcaagg aaaaaggcgg caacgtaacg
     2761 cgtgaagcgg gccccatgaa acacggtcaa accgtcatcg ccttcgtcga agaccccgac
     2821 ggctacaaga tcgaactggt ccagcacaaa ccgcagaccg tctaatcatc ggcgcgagcg
     2881 aacacaccgg cggcgaaatc atcga
//
LOCUS       DATCXU010000496         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_136737,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000496 DATCXU010000000
VERSION     DATCXU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136737"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VIR04_11150"
     CDS             <1..450
                     /locus_tag="VIR04_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY9461344.1"
                     /translation="ALGGGFELALSCDMVVASENAVFGFPEINLDTLPGWGGTQFAPK
                     KMGLNRAKEMVLTGNYYPAAVCVGFGFINRLVPTAQLRSATFELAKPIASKNAFALKM
                     AKSALQQAVGSDLKAGMRYEALAYAANFSMPHAKAGLNAFLDRKVKH"
     gene            574..1473
                     /locus_tag="VIR04_11155"
     CDS             574..1473
                     /locus_tag="VIR04_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY9461345.1"
                     /translation="MRHTWRAMFGERQRFMLMLIAPAVTLLILFQAVPIFIGGSASFQ
                     DWMLYNPKKTWLGLSHYISVLTDPAFLKIVLPNTFLFMFSSVAISLAFGLALAIVLNR
                     PFRGRKVVQTVLLLPLMVAPVIAAIMVRWMFNDQFGIVNVLFEALGGTPQPWLIQRWS
                     AFAIIVLTDCWLWIPWFTLLLLAGLQSLPKEPFEAAAIDGTNRWRVFRYLTVPMLRPV
                     IVVCIVIRAIDAFRTFDIVWALTGGGPARQTELFSVYAYEQAFVFLDFARGSAAAIIG
                     ALIILVVGLALYRVLEHVKKVSQ"
     gene            1470..2321
                     /locus_tag="VIR04_11160"
     CDS             1470..2321
                     /locus_tag="VIR04_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY9461346.1"
                     /translation="MSTNYSMSDSRSGFFGSMAPGARRAFFGLTIAAVCLLFAFPILW
                     LLLTSLRPESGIYYVYRGLDFTLDSYLQVLHDARLVQAFTNSGIIATLATVFSLLVTV
                     SSGYMLSRFRGPIATSWFGTIYALRCVPYITWVLPLYFVEQSYGVYDSYLGLLLPHVA
                     VHICFFTWLMKGFFDGIDSSMESAALIDGCTRWGAFYRVALPSAVPAISALAVLCWLF
                     TWNEFLFALILTGENTPVITVVMAQFVSEMGMQWNLMAATSMLALVPALLLTVFGQKY
                     VVRGLRI"
     gene            2412..3710
                     /locus_tag="VIR04_11165"
     CDS             2412..3710
                     /locus_tag="VIR04_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY9461347.1"
                     /translation="MKKAKQWLVAGAAGLVLCAGGSGAMAAQQELRIAYQPNPLQEAS
                     IDMMVKWGAKNNVKIIKVPNSYGVYVEKMTASLTSGSKQYDVIWHNDDWGQLWAHLLE
                     PLDDVAGLKYADKWSTDPIIFNNKDGKTTVVPMGETLGALFYRTDLVKESELPKTWDQ
                     LVTLSKKLQAEGKVKYGFVGGMNKDADWNSTLWTLWNNNCDVMLPFTSRDNAVLAKNG
                     WKSAMNEPCMRQVAEFWSDALNKDKISPPGMPAYGRNEANAIFTVGDAAFTVADTTYW
                     NDFNNPAKSKVAGKVGLAQFPLGPMGKDVRNWDEIWGWAIPKTVSAEHKALAKKMLSD
                     MMLDEEGQIALWKKTGAPPPNVTFWDKIAATDTFMQQLKKYYLDSPHKVHSAYYFSDW
                     PAVHKAYNDAMTTAITGNRADIPKALATGATNIHSAIKQK"
     gene            3769..>3917
                     /locus_tag="VIR04_11170"
     CDS             3769..>3917
                     /locus_tag="VIR04_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9461348.1"
                     /translation="MSQIVLSKVNKHYGGVQHAVKDIDLTVGDKEFVVLVGPSGCGKS
                     TTLRM"
BASE COUNT          825 a          947 c         1177 g          968 t
ORIGIN      
        1 gccttgggcg gaggattcga gcttgctctt tcgtgcgata tggtggtggc ttccgaaaat
       61 gctgtatttg gttttcccga gattaatctt gatacgttgc cgggctgggg cgggacacag
      121 ttcgcgccaa aaaagatggg actgaatcgc gccaaagaga tggttttgac gggcaattat
      181 tatcccgcgg ccgtttgtgt tggctttggt tttattaacc gccttgtgcc aacggctcag
      241 ttgcgttccg caacgttcga acttgccaag ccaattgcat cgaagaacgc ctttgcgctc
      301 aaaatggcca agagcgcctt gcaacaggcc gtcggatccg atctgaaggc aggcatgcgc
      361 tacgaggcct tggcttatgc cgcgaatttt agtatgccgc acgcgaaggc gggccttaac
      421 gcctttcttg atcgaaaggt gaagcattga tcgcatgatc ggatgtcaag aagtacgcta
      481 gttgcagtaa aaaaataata taaggagaca agttatgggt aagagtgatg gcggtttgca
      541 gtttttgaac gtccccggat ggagccggtg acgatgaggc acacttggcg agcgatgttt
      601 ggcgaacggc aacggttcat gcttatgctg atcgctccgg cggtcacgtt gctgattctg
      661 tttcaggcgg tgccgatctt tatcggcggc agcgcaagct ttcaggactg gatgttatac
      721 aaccccaaga aaacctggct ggggctgtcc cattacatta gcgtgctgac cgatccggcg
      781 tttctgaaga tcgtgttgcc taatacattc ttgttcatgt tcagcagcgt ggccatctca
      841 ttggccttcg ggctggcgct ggcgatcgta ttgaatcgtc cgtttcgcgg ccgcaaggtt
      901 gtgcaaaccg tgttgctgct gccgctgatg gtagcgccgg taattgcagc catcatggtg
      961 cgctggatgt tcaacgatca attcggcatc gtgaatgtgc tttttgaagc ccttgggggt
     1021 acgccacaac catggttgat tcagcgctgg tcggcctttg ccatcattgt gctgacggat
     1081 tgctggctat ggataccgtg gtttacgctc ttgttgctgg ctggcctgca gagcctgcca
     1141 aaggagccct tcgaagccgc cgcgatcgat ggcacgaacc ggtggcgcgt gtttcgctac
     1201 ctgactgtgc caatgctgcg cccggtgatt gtggtgtgta ttgtgatacg ggcgatcgat
     1261 gcgtttcgca ccttcgatat tgtgtgggcg cttacaggcg gcgggcccgc tcgtcagacc
     1321 gagctattta gcgtttatgc ctacgaacaa gcttttgtgt ttctggattt cgcgcgggga
     1381 tcggcggcgg cgattattgg agcactcatt atcctggtgg tgggcctggc tctgtatcgg
     1441 gttcttgaac acgtaaaaaa ggtgtctcaa tgagcacgaa ctattccatg agcgacagcc
     1501 ggtcaggttt ttttggttca atggcgccgg gtgcgcgccg ggcgtttttc ggcctgacga
     1561 tcgccgcagt ctgccttttg tttgcttttc ccattttgtg gctgctgctg acctcgctgc
     1621 ggccggaatc gggcatctac tacgtatacc gggggctgga ctttacgctg gacagctact
     1681 tgcaggtgtt gcacgatgcg cgcctggtgc aggcgttcac caatagcggc atcattgcga
     1741 cgctggcgac ggtgttttcg ttgctggtga cggtgtccag cggctatatg ctgtcgcgct
     1801 ttcgcggtcc catcgccacg agctggttcg ggactattta tgcgctgcgt tgcgtgccgt
     1861 atatcacgtg ggtgctgccg ttgtatttcg tcgagcaaag ctacggcgta tacgattcat
     1921 acctgggttt gttattgccg cacgtcgccg ttcacatctg tttcttcacg tggctgatga
     1981 aggggttttt cgatggcatt gactcgtcga tggagtcggc cgcgctaatt gacgggtgca
     2041 cgcggtgggg tgcattttac cgggtggcgc tgccttcggc ggtgccggcg atttcggcat
     2101 tggccgtatt gtgctggctg tttacctgga acgaattctt gtttgcgctg attttgacgg
     2161 gcgaaaatac gccggtcatc acggtggtga tggcgcagtt tgtgtctgaa atgggcatgc
     2221 agtggaactt gatggcggcg acctcgatgc tggcgcttgt gcctgcattg ctgctgacgg
     2281 tattcggaca gaaatatgtt gtgcgaggac tcagaatcta gtaatggtac gtatgggacg
     2341 gctaagtgtg cctatgccgt ttttttagtt ggctataaat agaaggtcaa tataacctca
     2401 aggaggtaga catgaaaaag gcaaaacagt ggctcgtggc aggcgccgca gggcttgtgc
     2461 tatgcgcggg cggtagtggt gcaatggctg cgcaacagga attgcgcatc gcctatcagc
     2521 ctaatccgct gcaggaagca tcgatcgaca tgatggtgaa atggggcgcg aaaaataacg
     2581 tcaagatcat caaggtgccg aattcttatg gcgtgtacgt cgaaaaaatg acggcgtcgc
     2641 tgacatcggg ttcaaagcag tacgacgtca tctggcacaa cgacgactgg ggacagctat
     2701 gggcgcactt gcttgaaccg ctcgatgatg tcgccggcct gaagtacgcc gacaaatgga
     2761 gcaccgatcc gatcatcttc aacaacaagg acgggaagac caccgtagtg cccatgggcg
     2821 agaccctggg tgcgcttttt taccgcacgg atctggtcaa ggaaagcgaa ctgcccaaga
     2881 cgtgggacca attggtcacc ctgagcaaga agctgcaggc cgagggcaag gtcaagtatg
     2941 gttttgtcgg agggatgaac aaggacgccg actggaactc cacgctttgg accttgtgga
     3001 acaataattg cgacgtcatg ttgccattca ccagtcgtga caatgcggtg ctggccaaga
     3061 atggctggaa gagcgccatg aatgaaccct gcatgcgcca ggttgctgaa ttctggtccg
     3121 acgcgctgaa caaggacaag atcagcccgc ccggtatgcc cgcttatggc cgtaacgagg
     3181 cgaatgccat atttaccgtg ggcgatgccg cgttcaccgt cgccgatacg acgtattgga
     3241 atgattttaa caatcctgcc aaatccaaag tggccggaaa ggttggtctt gcgcaatttc
     3301 cgcttgggcc catgggcaaa gacgtaagaa actgggatga aatctggggc tgggcgattc
     3361 cgaagactgt ttcagcggaa cataaagcgt tggccaagaa aatgttgtcc gacatgatgc
     3421 tggacgaaga aggccagatc gcgctttgga aaaaaaccgg tgcgccaccg cccaacgtga
     3481 cgttctggga caagatcgct gcgaccgaca cctttatgca acagttgaaa aaatactatc
     3541 tcgatagtcc tcacaaggtg cattccgctt attatttttc ggattggccc gctgttcaca
     3601 aagcctataa cgatgcgatg actacggcga ttaccggcaa ccgcgccgat attcccaagg
     3661 cattggctac cggcgctaca aatattcata gcgccataaa acagaagtaa tagctccatt
     3721 cgcgccgggg gcggttcccg gcgctttctt caaacgagag cggtcacaat gagtcaaatt
     3781 gttcttagca aggtgaacaa gcattatggc ggtgtgcagc atgccgtcaa ggatatcgat
     3841 ctgacagtgg gtgacaaaga gttcgtcgtc ctggtgggcc cttcgggctg tggcaagtcg
     3901 acgacgctgc gcatgat
//
LOCUS       DATCXU010000497         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_997202,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000497 DATCXU010000000
VERSION     DATCXU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997202"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VIR04_11175"
     CDS             complement(<1..573)
                     /locus_tag="VIR04_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEY9461349.1"
                     /translation="MYTLIISVACSVAVSVLLKIARRQTIHIGQAIAINYVVAAGLCL
                     LLFQPRPASLLNSSTPWWVLIALGLLLPTIFLAMAGAVRHAGIVLSDAAQRLSLFIPL
                     IAAFALFGEAQSSGKLVGIATALVALICLLFRPGAQHESSEKGKAISLLLCVWVGYGA
                     IDILFKQLAKSGAIFSSSLFVSFVLAGIVIF"
     gene            complement(587..1612)
                     /gene="bioB"
                     /locus_tag="VIR04_11180"
     CDS             complement(587..1612)
                     /gene="bioB"
                     /locus_tag="VIR04_11180"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY9461350.1"
                     /translation="MTTRHSTLPSTTTQDQHWSVEEIIELYELPLLDLIFQAQTVHRR
                     HHEHNAVQLSSLLSIKTGGCPEDCAYCPQSARYNTEVGREALMPLEQVLQAARQAKDN
                     GAQRFCMGAAWRSPKAQHIEAIAEMITAVKEMGLETCATLGMLKDGQAEQLKDAGLDY
                     YNHNLDTSPAFYGKVITTRTYQDRLDTLQRVRHAGLNVCCGGIVGLGESRRDRAGLIA
                     QLANLSPYPESVPVNNLVKVAGTPLCDAEQIDPFEFVRTIAVARITMPKAIVRLSAGR
                     QEMNDSTQALCFLAGASSIFYGEKLLTTGNPQFTQDQALFQRLGLKPKSPAASNYALS
                     VHMKNIG"
     gene            complement(1802..>2212)
                     /locus_tag="VIR04_11185"
     CDS             complement(1802..>2212)
                     /locus_tag="VIR04_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY9461351.1"
                     /translation="AAVVLGGIGYAGGGKASRTLGGWQTTCWCLAISMPFLTLPTFYL
                     TMNLPSWPPLSILGAFAYLAFGSMFLGFFAWYKGLAMGGIARVGQLLLIQPFLTVVAS
                     AWLFGERVPLATYLFALAVIVIIGASRYVTVNHS"
BASE COUNT          498 a          608 c          633 g          473 t
ORIGIN      
        1 aaagatgacg atgccggcaa gaacgaaact gacaaacaag ctgcttgaaa atatggcgcc
       61 gctttttgca agctgcttga acagaatgtc gatcgcgcca tagccgaccc atacgcatag
      121 cagcagactg atcgccttgc ctttttcgct tgactcgtgt tgcgctcccg gccgaaacag
      181 cagacagatc agcgcgacca gagcggtggc aattcccacc agtttgccgc ttgactgcgc
      241 ttcgccaaac aaggcaaagg cggcaatcag cggtatgaat agcgagagcc gctgcgccgc
      301 gtcgcttagc acgatacccg catggcgcac tgcaccggcc atcgccaaaa aaatagtggg
      361 caataacaag cccagcgcga tcagcaccca ccatggcgtg ctggaattta gcagcgacgc
      421 gggccggggt tgaaacagca acaggcacaa gccggccgcc accacgtagt tgatggcgat
      481 ggcttgcccg atgtgtatgg tttgccggcg cgcgattttt agcagcaccg agaccgccac
      541 gctgcaggcc acgctaatta tcagggtgta catagagccg ggcggtttat ccgatgtttt
      601 tcatgtgtac gctcagagcg taattcgacg cggcgggact ttttggcttt aaacccaggc
      661 gttgaaaaag tgcctggtct tgcgtgaatt gcgggttgcc ggtggtcagc agtttttctc
      721 cgtagaaaat cgaactcgcg cccgccagaa aacaaagcgc ctgagtggaa tcgttcattt
      781 cctggcgccc cgcggataag cgcacgatcg ccttgggcat tgtgattcgg gcaaccgcga
      841 tagtgcgcac gaattcaaac ggatcgattt gttccgcgtc gcataaaggc gttcccgcca
      901 ccttcaccag attgttcaca ggaaccgact cggggtaggg cgacagattg gcaagctggg
      961 cgattagtcc ggcgcggtca cggcgagatt cgcctagccc gactatgccg ccgcaacaaa
     1021 cattcaatcc ggcgtggcgt acccgttgca gggtatccag ccgatcctga taggttcgcg
     1081 tggtgatgac tttgccgtag aatgcgggcg acgtgtccag gttatggttg tagtagtcaa
     1141 gcccggcatc ttttaattgt tcggcctggc cgtcttttaa catacccagc gtcgcacacg
     1201 tttccaggcc catttcttta accgccgtga tcatttccgc aatggcttca atgtgctgcg
     1261 cttttggcga acgccatgcc gctcccatgc aaaaacgctg tgccccgtta tctttggctt
     1321 gccgcgcagc ctgaagcact tgctccagcg gcataagcgc ctctcttcca acttctgtgt
     1381 tgtagcgcgc cgactgcggg caataggcgc agtcttccgg gcagccgccg gtttttatcg
     1441 ataacagact ggataattgc acggcgttgt gttcatggtg gcggcggtgc acggtttgcg
     1501 cctgaaagat caaatccagc agcggcaatt cgtaaagttc gattatttct tcgaccgacc
     1561 aatgctggtc ttgggtcgtc gtggagggta gcgtggaatg ccgagtagtc atgaatagga
     1621 gccagcatgt ggtgaacctt cgcatggtag ggtcggcgcc cctcttgacg caactgtctc
     1681 gatggcacta agttgctcga tattggcgac gtttatcggc atttaggcca aagatgcccg
     1741 cttaaaacgt gactgcctgc ttaatcccgt taaatcgatc gccagttgaa gttgtatctg
     1801 tttatgaatg attgacggtg acgtagcggc tggcgccaat aatcacgatc acggccagag
     1861 cgaataaata ggtggccagc ggtacacgtt ccccgaaaag ccatgcggag gcgacaaccg
     1921 tcaggaaggg ttgtatcaac agtagttgac ccacgcgggc gataccgccc attgccagcc
     1981 ctttgtacca ggcgaagaaa cccaggaaca tggatccgaa ggccagatag gcgaaggcgc
     2041 ccagaatgga cagcggaggc cacgaaggaa gattcatggt cagataaaag gtgggcagtg
     2101 tcagaaaggg catactgatt gccagacacc agcaggtggt ttgccagccg cccagcgtgc
     2161 gggaggcttt cccgcctccc gcataaccaa ttccgcccag taccacggcg gc
//
LOCUS       DATCXU010000498         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_924411,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000498 DATCXU010000000
VERSION     DATCXU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924411"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VIR04_11190"
     CDS             complement(<1..521)
                     /locus_tag="VIR04_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY9461352.1"
                     /translation="MNRALRNVAIIAHVDHGKTTLVDQLLRQSGTFRENQHVTERVMD
                     SGDIEKERGITILAKNCAVEYEGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVVNKVDRNGARPSHVINATFDLFDKLGATEEQLDF
                     PVIYASGLDGYAGL"
     gene            complement(518..1273)
                     /gene="truB"
                     /locus_tag="VIR04_11195"
     CDS             complement(518..1273)
                     /gene="truB"
                     /locus_tag="VIR04_11195"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY9461353.1"
                     /translation="MASRRGQELDGVLLLDKPVGLSSNHALQRAKHALDARKAGHTGT
                     LDPFATGLLVCCLGRATKMAASMLDADKTYRAIVRFGQETDTGDLTGVAVFEALRPFS
                     GVERRDLDNVLVNFRGEIDQVPPMYSALKRDGKPLYKYARQGIELDRASRKTVIYKLE
                     ALSFSGLEAELEVRCSKGTYIRTLAQDIGRALGCGAHLVALRRTQVGPFVLDDAVALD
                     ALQAMPLPQSVLIALDALPAGLLPAARKQKDEL"
     gene            complement(1308..1718)
                     /gene="rbfA"
                     /locus_tag="VIR04_11200"
     CDS             complement(1308..1718)
                     /gene="rbfA"
                     /locus_tag="VIR04_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY9461354.1"
                     /translation="MSRHKSKPNPGRNTRLADQIQKDLAGLIQREVDVARAGLVTLTG
                     VELSADYAHAKVYFTVMGAEPEQATSALAEKAGWLHSLLFKLLQIHTVPTLRFIHDDQ
                     LARGLALSRLIDQANSPWVPPSSRLDPDNPDGQP"
     gene            complement(1721..>3748)
                     /gene="infB"
                     /locus_tag="VIR04_11205"
     CDS             complement(1721..>3748)
                     /gene="infB"
                     /locus_tag="VIR04_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY9461355.1"
                     /translation="AAAKGAGKKEGKAGAGASDIKKVIKTSDVSSSWADDSGRKKPAA
                     KADTPPSSPSRDGWRAGKAGKGAGRARGGRNRNAQPEHKEPQPIEFIAREVHVPETIS
                     VADLAHKMSIKAAEVIKHLMKLGQMVTINQVLDQETAMILVEELGHIAIAAKLDDPEA
                     FLDADADEQAQAEALPRAPVVTVMGHVDHGKTSLLDYIRRTKVASGEAGGITQHIGAY
                     NVKTSKGTVTFLDTPGHEAFTAMRARGTKATDIVILVVAADDGVMPQTKEAIHHAKAA
                     GVPLVVAINKIDKPDANPERVKQELVTEEVVPEQYGGDVPFIPVSAKTGAGIDDLLDS
                     VLLQAEMLELKAPVEAPAKGVVIEARLDKGRGPVATILIQSGTLSRGDAVLVGASYGR
                     VRAMLNENGKPISSAGPSIPIEIQGLTEVPAAGDELIAMADERKAREIALFRQGKFRD
                     VKLARQQAAKLESMFDNAGEGMQTLALIVKTDVQGSQEALVSSLVKLSTNEVRVQVVH
                     AAVGGISESDVNLAIASHAVVIGFNVRAELSARKLAEHNGIDLRYYNVIYDAVDDVRN
                     AMSGMLAPEKREEVIGMVEVREVFTISRVGTIAGCMVVDGLVHRDSQVRLLRANVVHW
                     TGWIDSLRRFKDDVKEVKSGFDCGITLKGNNDIQVGDQLEVFEIKEVARTL"
BASE COUNT          779 a         1149 c          988 g          832 t
ORIGIN      
        1 aggcctgcat agccatccag gccggacgcg tatatcaccg gaaagtccag ttgttcttcc
       61 gtggcgccca gcttgtcgaa taaatcaaag gtcgcattga tgacatggct ggggcgtgcg
      121 ccgttgcgat caaccttatt gacaaccaca atgggtttca gacccaaggc cagggccttg
      181 cgcgtcacaa agcgggtttg cggcatcggg ccctcaaccg catcgaccag caacaacacg
      241 ccatcgacca tcgacaatac gcgctcgact tcgccgccga aatcggcgtg acccggagtg
      301 tcgacgatat taatgtgcgt gccttcatat tcaacagcgc agttcttcgc caaaatcgtg
      361 atgccgcgtt ctttttcgat atcgccggaa tccattacac gttcagtcac atgctgattc
      421 tcgcgaaacg tgcccgattg gcgcagcagt tgatcaacca gcgtggtttt accgtggtcg
      481 acgtgggcga taatcgccac attgcgcaaa gcgcgattca taattcatcc ttttgtttac
      541 gggctgcggg caataagccg gcaggcaaag catcaagtgc tattaacacg gactggggca
      601 gcggcattgc ctgcaacgca tccagtgcca ccgcgtcgtc caagacaaac ggccccacct
      661 gtgtgcggcg caatgcgacc agatgggcgc cgcatccgag cgcgcgtcca atatcctggg
      721 cgagtgtgcg aatgtacgtc cccttgctgc aacgaacctc aagttcagct tcgagtccac
      781 tgaagctcaa cgcttccaac ttgtaaatca cggtcttgcg cgacgctcga tccagctcta
      841 taccctgacg cgcatattta tatagtggct tgccatcgcg ctttaacgcg gaatacatcg
      901 gcggaacctg atctatctca ccacgaaaat tgaccagcac attgtccaaa tcacgccgtt
      961 cgacgccgga aaaaggtctt agcgcctcga acacggcgac gccggtcaga tcacccgtat
     1021 ccgtctcctg tccgaaacgc acaatggccc ggtacgtctt gtcggcgtca agcatgctgg
     1081 cggccatctt ggtcgcacgt cccaggcaac acaccagcaa gcccgtcgca aacggatcaa
     1141 gagtgccggt gtggccagcc ttccttgcat ccagcgcatg cttggcgcgc tgcagagcgt
     1201 gattgctgga caaccctacc ggtttatcca gcaacaacac gccatcgagc tcctgcccgc
     1261 gtcgggaagc catcgtttat caagccttat ataaaaacga cagctctcta cggttgcccg
     1321 tcaggattgt cagggtcaag acgagacgaa ggaggaaccc aagggctatt cgcctgatca
     1381 ataagccgtg ataatgccag gccgcgcgcg agctgatcgt catgaataaa acgcagggtc
     1441 gggaccgtat ggatttgcaa cagcttaaac aacaatgaat gcaaccagcc ggccttctcg
     1501 gccaacgcac tggttgcctg ctcgggctca gctcccatga cagtgaaata cactttcgca
     1561 tgggcgtaat ccgccgataa ttcgactccc gtcagggtaa caagccccgc ccgcgcgacg
     1621 tccacttcgc gctggatcag gcccgccaga tctttctgga tttggtcggc cagccgggta
     1681 ttgcgacccg ggttcggttt ggacttatgg cggctcatgc ttataaagtc cgggcaactt
     1741 ctttgatttc aaagacttcc aattggtcgc ccacttggat atcgttattg cctttgagcg
     1801 taatgccgca atcgaagccc gacttgactt ctttgacatc atctttaaac cgccgcagag
     1861 agtcgatcca gccggtccag tgcaccacat tggcgcgcaa caggcgcact tgtgaatcgc
     1921 gatgcaccaa tccgtcgacg accatgcatc ccgcgatcgt tccgacccgt gaaatggtga
     1981 atacctcgcg gacttcgacc atgccgatga cctcttccct tttctcgggc gccagcatgc
     2041 ctgacatggc gttgcggaca tcgtcgacgg catcgtagat aacattgtaa taacgcagat
     2101 cgatgccgtt gtgctcggcc aattttctgg cgctcagttc ggcgcgcacg ttaaacccga
     2161 tcactacggc atgcgaagca atcgccaaat tgacgtcgct ctctgaaatg ccgcctacgg
     2221 ctgcgtgcac cacctggacc cgcacttcgt tggtcgataa tttaacaagg gatgacacca
     2281 gcgcctcttg cgaaccctgc acatcggtct tgacgatcaa ggccagggtc tgcattccct
     2341 cgccggcatt atcgaacatg gattcaagct tggccgcctg ttgccgcgcc agtttgacgt
     2401 cgcggaattt gccctggcgg aacaatgcga tttctcgcgc tttgcgttca tcagccatgg
     2461 caataagttc atcgccagca gcgggaacct cggtcagccc ctgtatttcg atcggaatcg
     2521 atgggccagc gcttgaaatt ggcttgccat tttcgttcag catcgcgcgc acgcggccgt
     2581 aacttgcacc caccaaaacg gcatcgccgc gcgataaagt gccgctttga atcagaatag
     2641 tcgccaccgg accgcggccc ttgtcgaggc gagcctctat gacgaccccc ttggccggcg
     2701 cctcgaccgg agccttgagt tccagcattt cggcttgcaa caggacactg tcgagcaaat
     2761 cgtcgatacc tgcgccagtt ttagccgaca ccggaatgaa ggggacatcg ccgccgtact
     2821 gttccggtac gacttcctcg gtgaccaatt cttgcttgac ccgctcggga ttggcatccg
     2881 gcttatcgat tttgttaatt gcaacaacca ggggcacgcc cgccgccttg gcatggtgaa
     2941 tagcttcttt ggtttgcggc atgacgccat catcggccgc gacaaccaga atgactatgt
     3001 ctgttgcttt cgttccccgg gcgcgcatgg ccgtaaaggc ctcatggccc ggagtgtcca
     3061 gaaatgtcac cgtacctttg ctggttttga cgttgtatgc gccaatgtgc tgggtaatgc
     3121 cgcctgcttc gcccgaggcg actttggtgc ggcgtatgta atcgagcaac gacgtctttc
     3181 catgatcgac gtgacccatc accgtaacga caggcgcgcg cggcaaagcc tcggcctgag
     3241 cctgctcatc ggcatcggca tccaggaagg cctcgggatc gtccagcttg gctgcaatcg
     3301 cgatatggcc gagctcttca accagaatca tggccgtttc ctgatcaagc acctgattga
     3361 tcgtcaccat ctggcccagt ttcataaggt gtttgataac ctcggcggcc ttgatcgaca
     3421 ttttgtgggc caaatcggcc accgaaatag tctccgggac atgcacctcg cgagcgataa
     3481 attcaattgg ctggggctct ttatgttcgg gctgcgcatt cctgttgcga ccaccacgag
     3541 cccgtcccgc gcccttgcct gccttgccgg cgcgccatcc atcgcggctc ggactggacg
     3601 gaggcgtatc ggccttggcg gccggtttct tccggccact atcatcggcc caggaggacg
     3661 agacgtcgga cgtttttata accttcttga tatcggatgc gccagcgccc gcctttcctt
     3721 ccttcttgcc ggcgcctttg gcggcggc
//
LOCUS       DATCXU010000499         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_896367,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000499 DATCXU010000000
VERSION     DATCXU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896367"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VIR04_11210"
     CDS             complement(<1..153)
                     /locus_tag="VIR04_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY9461356.1"
                     /translation="MPLKDWLAALLVVVIWGINFVIIKVGLNDVPPLLLGGLRFSLVA
                     FPAVFFV"
     gene            191..1147
                     /locus_tag="VIR04_11215"
     CDS             191..1147
                     /locus_tag="VIR04_11215"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY9461357.1"
                     /translation="MCTVWGIERYYLPSGIAVPAHINIRSRHPMSVVISGLYTYPVKS
                     CAGIAHMHSRITQAGLAFDRRWVMVDHRGIFMTQRTCARMALIRPSVAPDGLTLDAPG
                     MPAITLLPTADNAVPAQIPVRIFDTDTIGSDEGDDVARWLSLFLHVRCRLLRMHSQAA
                     RIASPEYVDRWIGKNNVWEANFPTRHVFGFADAFPFLIINQASLDELNQQLQAKGHAA
                     VPMNRFRPNIVLQGLPSYDEDYLKGMRINGMTFAFVKRCARCPIPNINQETAVSADEP
                     NRTLIANRSFTDGVLFGVNAVVAHARDATLNVGDPVEIEYDL"
     gene            complement(1152..1811)
                     /locus_tag="VIR04_11220"
     CDS             complement(1152..1811)
                     /locus_tag="VIR04_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9461358.1"
                     /translation="MNFADLMVLAAPILLKGTGYTLIFAFSAMLGGLLIGFAISVARI
                     IPNRAVQWPAILYVSMMRGTPLLVQVFIVYYGLPSIGISFDPLTAGVLALSLNAGAYI
                     SESLRGAILGISRGQWSAGYSLGLTYWQNLSYIVLPQALRLAVPSMSNTLISLIKDTS
                     LVSVITVTELLLATKDVIATTFQPLPLYLAAAGIYWVLSLGLERVQSHLEKYYGRAQS
                     A"
     gene            complement(1910..2719)
                     /locus_tag="VIR04_11225"
     CDS             complement(1910..2719)
                     /locus_tag="VIR04_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461359.1"
                     /translation="MKLSRYARWIAASSAVMFAALAASPAYSADLLDTVKDRGTLIVG
                     MEGTYPPFNFVDTKTHQLEGFDVDVSRLIASKLGVKVEFVKTEWSAILAGLSSGKFDV
                     IVNQVGITPEREKTFDFSIPYVASSPQLILRKDDSSHYKSFADLAGKKLGVSQGSNYE
                     ALAKAQKGVTVKSYPGAPEYLSDLVNKRVDAALNDQLMTAYLVKTSSIPIKGGAIVGD
                     PKFNGIPFRKGNPKFKAAINQALEDSFKDGEFAKISNKWFGIDVSKVQAAQ"
     gene            complement(2840..4000)
                     /gene="dinB"
                     /locus_tag="VIR04_11230"
     CDS             complement(2840..4000)
                     /gene="dinB"
                     /locus_tag="VIR04_11230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY9461360.1"
                     /translation="MSGPARRIAHLDMDAFFASVELLRYPELRGLPVVVGGRDTAQPQ
                     VQADGTRRFFHLRGYVGRGVVTTSTYEARAFGVFSAMGLMKAAQLAPDAILLPADFPS
                     YRHYSQLFKAAVAQVAPCIEDRGIDEIYIDLTGHPMPSLELAQSIKEAVKRATGLSCS
                     IAITPNKLLSKIGSELDKPDGITILDFDDVPRRIWPLSVRKVNGIGPKAGAKLAALNI
                     MTVADLARAAPALLQDHFGLSYARWLLDVARGIDDRPVRTHSEPKSISRETTFEHDRH
                     VVHDRAILSEVFSDLCTRLAGDLQRKGYVGRTIGIKLRFDTFQTVTRDITLLAPTADA
                     ATIHRAAGACLKRIRLDHKIRLLGVRASSLQPRGQQPAVDGLSGHDAQLSLY"
     gene            complement(4060..5040)
                     /locus_tag="VIR04_11235"
     CDS             complement(4060..5040)
                     /locus_tag="VIR04_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY9461361.1"
                     /translation="MTKARVIQIEQFGTPDVMRVATVDLPAPGAGQVQLRQTAVGFNY
                     IDIYQRQGVYPLPLPTGLGHEAAGVVRAVGQGVRNVREGDRVAYMNAGVGAYADYRNV
                     PADKLVMIPDTITDEQAAALIFKGMTAQYLLRRTYAVQPGDMVLVHSAAGGVGQILSA
                     WAKALGAYVVGTAGSDAKCEAARTAGCDVAINYSQPDWPKTLLAATGGRKARVVYDGV
                     GKDTFLKSLDCAAPFGLVVYYGAASGPAPAIEPELLNKKGCLFLTRPSVFPHNADPVR
                     FRENAADLFAAADSSMFQVHIGARFPLEEAALAHLAASNRETTGSIVLIP"
     gene            complement(5042..>5246)
                     /locus_tag="VIR04_11240"
     CDS             complement(5042..>5246)
                     /locus_tag="VIR04_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEY9461362.1"
                     /translation="DGQEEIAGAYLAPDSLSRAWNLGVNPKNALKNNDGHGFFQALGD
                     SIVTGPTYTNVNDFRAILIVDGR"
BASE COUNT         1227 a         1611 c         1348 g         1060 t
ORIGIN      
        1 gacgaaaaaa acggccggga aagccaccag agaaaaccgc agcccgccca gcaacaaggg
       61 tggcacatcg tttaacccaa ctttgattat gacaaaattt atgccccaaa taacaactac
      121 aagtaatgcg gccagccaat ctttcaaggg cattgtggat cctgctcggt tattcccaat
      181 ttgaattgca ttgtgcaccg tttggggtat tgagcgctat tatctgccca gcggcattgc
      241 cgtgcccgcg cacataaata tccgatcacg ccatcccatg tcagtcgtca tctccggtct
      301 gtacacctat cccgtgaaat cctgcgccgg catcgcgcac atgcattcac ggatcacgca
      361 ggcagggctt gcattcgacc ggcgctgggt aatggtggat caccggggta tcttcatgac
      421 gcagcgaacg tgcgcccgca tggctctgat tcgacccagc gtcgcgcccg atggtctgac
      481 gcttgatgca cccggcatgc ccgccattac cctgttgccg acggcggaca acgccgtgcc
      541 cgctcagatt cccgtgcgca tcttcgacac agacaccatc ggatccgacg agggcgacga
      601 cgtggcgcga tggctaagtc tgttcctgca tgtgcgctgc cgcctgttgc gcatgcactc
      661 ccaggccgcc cgaatcgcca gccccgaata cgtggaccgc tggatcggaa aaaacaacgt
      721 ctgggaagcc aattttccga cccggcacgt tttcggcttt gccgacgctt ttcccttcct
      781 gatcatcaac caggcttcgc tggacgaact gaatcagcaa cttcaggcga aaggacacgc
      841 cgccgtgccc atgaatcgct ttcggccgaa catagtcttg cagggcctgc catcctatga
      901 tgaagactac ttgaagggca tgcgcatcaa cgggatgact ttcgccttcg tcaaacgttg
      961 cgcgcgctgt ccgattccga atatcaacca ggaaaccgcc gtgtcggccg atgaaccgaa
     1021 ccggacactg atcgccaatc gcagttttac cgatggcgtc ctgtttggcg ttaacgcggt
     1081 ggtcgcgcac gcgcgcgacg cgacgctaaa tgtgggcgac ccggtcgaga tcgaatacga
     1141 tctttagcct ctcaggccga ttgcgcccgc ccgtagtact tttcaagatg cgactggacc
     1201 cgctccaacc ccaggctcag gacccaataa atgcccgctg ccgccaaata caaaggcagc
     1261 ggctggaacg tggtggcaat cacgtccttg gtggcaagta gcaattcagt gacggtaatc
     1321 acagaaacca gcgaggtgtc tttaatcagg ctgatcagcg tgttgctcat tgacggcacc
     1381 gccagacgca aagcctgcgg cagcacaatg taagacaagt tttgccaata ggtcagcccc
     1441 aggctatagc ccgccgacca ttgtcccctg ctgattccca aaatggcgcc acgcaagctt
     1501 tcggaaatat acgcgccggc gttcaggctt aaagccagca cgcccgccgt caaagggtca
     1561 aacgaaatgc caatgctggg caagccataa tagacaataa acacctgaac cagtagcggc
     1621 gtaccacgca tcatgctgac atacagaatc gccggccact gaactgcgcg atttggaatg
     1681 atgcgggcca cggaaatcgc aaacccgatc agcaagccgc cgagcatcgc cgaaaaggca
     1741 aagatcaggg tatagcctgt tccctttaaa agtatcggcg cggcaagcac cataaggtct
     1801 gcaaaattca tagtccgccc gtaaacaaaa tccccgtaag cgctttatac gcctacgggg
     1861 atgaatccaa caattatttc cagcggcttg ttcgtcgcgg catcagagtt tactgagctg
     1921 cctgaacttt actgacgtcg attccaaacc acttgttgga aattttggcg aactcgccat
     1981 ccttgaaaga atcttccagc gcctggttaa tggccgcctt gaatttcgga ttgcccttcc
     2041 tgaaaggaat cccattaaat ttcggatcgc cgacaattgc gccgcccttg atcgggatgc
     2101 tggacgtttt gaccaaatac gccgtcatga gctgatcatt aagcgccgca tcgacccgtt
     2161 tgttgaccaa gtccgataga tattccggag cgcccggata gcttttcacg gttacaccct
     2221 tttgcgcttt cgccagcgct tcgtagttgc tgccctgcga tacgcccagc ttcttgcccg
     2281 ccagatcggc gaaactttta tagtgactgg aatcgtcttt acgcaggatc aattgcggac
     2341 tggacgccac ataagggatc gagaaatcaa acgtcttttc acgctctgga gtgatcccca
     2401 cctgattcac aattacgtcg aacttgccag agcttaaacc ggccaggatg gcgctccatt
     2461 ccgttttgac gaattcgact ttgacgccca gcttgctggc gatcaaccgc gagacatcca
     2521 catcaaaccc ctcaagctgg tgagttttgg tgtcgacaaa attaaaggga gggtaagtgc
     2581 cttccatccc cacgatcaaa gtgccgcgat ctttgactgt atccagcaaa tcggccgaat
     2641 aagccgggct ggcggccagc gccgcaaaca tcaccgcgct ggaagcggcg atccagcgtg
     2701 cataacgcga aagtttcatc atgtgtcctg ccaagtcatt aaacactcat gctaaccgta
     2761 aagccgccgc tttataacga tttcattttt acttacatat tacccggcga catatcaact
     2821 ggccggccga gttccataac taataaagac tcaattgcgc gtcatggccg gacaaaccat
     2881 ccacggccgg ctgttgaccc cggggttgca ggctgctggc ccgcactccc agcaggcgaa
     2941 ttttatggtc caggcgaatt cgcttcaggc acgcgcccgc ggcgcgatgt atcgtcgccg
     3001 catcggccgt cggcgccagc aaagtgatat cgcgcgtcac cgtctgaaag gtgtcaaagc
     3061 gaagctttat gccgatagtg cgccccacat aacctttgcg ctgcaaatcc cccgcaagcc
     3121 gggtgcacaa gtccgaaaac acttcagaca aaatggcgcg gtcatgaacc acgtggcgat
     3181 catgctcaaa cgtggtctcg cggctaatcg atttgggctc ggaatgcgtc cgcacaggac
     3241 gatcatcgat gccgcgcgcc acatccagca gccagcgcgc ataactcagg ccaaagtgat
     3301 cctgcaacaa ggcgggcgcc gcgcgcgcca ggtcggccac cgtcataatg ttcaacgccg
     3361 ccagcttggc gcccgccttg ggtccaatgc cattgacttt gcgcaccgac aaaggccaga
     3421 tccggcgcgg cacgtcgtca aaatcaagaa tggttatgcc atcgggcttg tccagctcgg
     3481 aaccgatttt cgagagcagc ttattggggg taatggcaat cgagcaagac agccccgtcg
     3541 cccgcttcac cgcttcttta atagactggg caagttccag cgacggcatg ggatgcccgg
     3601 tcagatcaat atagatctcg tcaatacccc gatcttcaat acagggcgcc acctgagcca
     3661 ccgccgcctt gaacaactgc gaatagtgac gatatgacgg gaaatcggcc ggcagcaaaa
     3721 tcgcatctgg cgccagttga gccgctttca tcagccccat tgcggaaaat accccgaacg
     3781 cgcgcgcctc gtacgttgat gtggtcacca cgccccggcc gacatagccg cgcaaatgga
     3841 agaaccggcg cgtaccatca gcctgaacct gcggttgcgc cgtgtcacgc ccacccacta
     3901 cgacgggcag tccgcgcaat tccggataac gcaaaagttc aacggacgca aaaaacgcat
     3961 ccatatccaa atgagcaatg cggcgggcgg gaccagacat aaattaaact gcatttttaa
     4021 acagtattta ttataggacg caaactcgcc gccgggcggc tatggaatca atacgatcga
     4081 acccgtcgtc tccctgtttg aggcggccaa atgcgccagg gccgcctctt ccaacgggaa
     4141 acgcgcgcct atatggacct ggaacatgga cgagtcggcc gcggcgaata aatcggcggc
     4201 gttttcgcga aacctgaccg gatcggcatt atgcggaaag acagaaggcc gtgtcaggaa
     4261 caggcaccct tttttattta atagctcggg ttcgatggct ggcgccggtc ccgatgcagc
     4321 gccatagtaa accaccagtc cgaacggcgc tgcgcaatcg agcgacttca gaaacgtgtc
     4381 cttgcccacg ccgtcataca ccacgcgcgc cttgcggccg cccgttgccg ccaacagcgt
     4441 tttaggccaa tccggttgcg aataattgat ggcgacgtcg cagcctgctg tccgcgcggc
     4501 ctcgcacttg gcatcagagc ccgccgtccc gacaacatac gcgccgagcg ccttggccca
     4561 cgcgctcaga atttgtccca caccgcccgc ggccgaatgc accagcacca tgtcgcctgg
     4621 ttgaacagcg tacgttcgcc ttaacaaata ttgcgccgtc atccccttga aaatcagcgc
     4681 ggcggcctgc tcgtcggtaa tcgtgtcggg aatcatcacc agtttgtcgg ccggcacatt
     4741 gcgataatcg gcgtaggcgc ccactccggc attcatgtac gccacgcgat ctccctcgcg
     4801 cacattacgc acaccctgac ccacggcgcg cacgaccccc gccgcttcgt gcccaagacc
     4861 ggtcgggagc ggcagcggat aaacaccctg gcgctgataa atatcaatat agttaaaacc
     4921 gacggcggtc tggcgaagct ggacctggcc cgcccccgga gccggaagat cgactgtcgc
     4981 gacgcgcatt acatcgggcg tgccgaactg ttcgatttga atcacacgtg cttttgtcat
     5041 gttatctccc atccactata aggatcgcgc gaaaatcatt cacattggtg tatgtaggcc
     5101 cggtgacaat ggaatcgccc aatgcctgaa aaaacccatg gccatcgttg tttttcaagg
     5161 cgttcttcgg attaacgccc aaattccatg cgcgcgaaag actgtcaggc gccagatacg
     5221 cgccggcaat ttcttcctgg ccatcc
//
LOCUS       DATCXU010000500         7422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_375160,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000500 DATCXU010000000
VERSION     DATCXU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375160"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VIR04_11245"
     CDS             <1..1202
                     /locus_tag="VIR04_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEY9461363.1"
                     /translation="GVAGDTVFVGLTLDLTGPFSAQGAEEKLGYQLAAEQLNAGAPEI
                     LKISPLTKKGVMGKRVELGVADAETKPNTAVQAATHFLHDNKAMLITGSSSSAVAIAL
                     QKVCGREHTLYMAAISASNDTTGKDCQRYGFRLGFFAYPVCKMIAPVLARSMGKNRKA
                     AYLVPDYTYGHTAFDSMKEFTEKEGWTTVTEQLHPLGATDYGSYLINIANSGADTLVV
                     LDFGADASNSIKQAKQFGLLDKMKITVPVMSSFLEKELGADLMQGVYAATGFWWTLED
                     KYPIAKDFVTAFEKKYNAKPRDSAYIAYLNLALWADAVERAKTFYPPDVIKAFEAGVV
                     RQGPVGDVTFRAQDHQGIINFPIVRGKKPSDMKGPDDFFEIVHVVEGKTALPEVGLLG
                     CKLGAYV"
     gene            1332..2393
                     /locus_tag="VIR04_11250"
     CDS             1332..2393
                     /locus_tag="VIR04_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY9461364.1"
                     /translation="MITYPSFKVAAMHVSPVFLDTEKTVDKVCALIDEAASAGAELIV
                     FPEAFVPAFPLWSSVRAPIFNHDFFRQLASNAVRVPGPEVKRVAQAAKRHNVTVSLGI
                     NEGTDASVGCIWNTNIIIGPNGVLLSHHRKIVPTYFEKLTWANGDGAGLRVVNTPLGK
                     LGMLICGENSNPLARFALMAQGEQVHIANYPPLWPNGGNYDIVQSIRARAQAHSIEAK
                     AFTVVSSAYLTDDVKELIADGDKRIREVMDNTPRSASMVIGPNGEQMGEIRQNEEGII
                     YADINIADCVVPKQFHDVVGYYNRFDIFKLTVNRSSNDPINFDAADQERGTVNTDLAA
                     FGEASASADSRSLVGQDIS"
     gene            2425..3651
                     /locus_tag="VIR04_11255"
     CDS             2425..3651
                     /locus_tag="VIR04_11255"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY9461365.1"
                     /translation="MSLTLSGLRVLDLTRALAGPFCTMALADLGAEVVKAEPTPSGDM
                     ARQWGPIDRDISVYYLSANRNKKGIGIDFRSAEGLALLRQLALKSDIVVENFRTGTME
                     KMGLGYESLARENPKLIMASISGFGNKGPAKDWAGFDQIAQGYSGFMSLTGTPETGPT
                     RVGVPIGDLTAGMWLTIGILAAIVERGRTGRGQHVETSLLAGLMSLLCVQGQRYLNLG
                     EVPEPCGNAHPVIAPYGTFETADGPLNLAPATEAMWVKLCSLLGASQLVDDPRFLTNV
                     ERRKNGDALTQELEARLREKTREEWTAIMIENGIPAGPINRMDDVFNDAQVAACNLVQ
                     TVEHSVLGAIKLVALPVQMGSAGDAPTVRSAPPLFGEHSQSILADYGFSSDEIQRLLD
                     SKVIRQAKAGAEKQDA"
     gene            3840..4613
                     /locus_tag="VIR04_11260"
     CDS             3840..4613
                     /locus_tag="VIR04_11260"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461366.1"
                     /translation="MKLSFKNIQKSFAGLDVIDNFSREMNDGELIALIGPSGCGKSTL
                     LHIAAGLEKPTSGVLLADGAPIAGPDPERTLMFQENALYPWLTLAQNVALALEFQKVD
                     KRDAREQALVWLEKVNLKGFADYYPHQVSGGMRQRVALARAFITRPKVLLLDEPFGAL
                     DALTRMTLQDALRELIREARPTVLLVTHDVDEALFLADRILVFSSRPATVLKEFNLAR
                     REKTHDLLEFAAMRREILELLGIHAKQVELTGLQEEMAA"
     gene            4610..5605
                     /locus_tag="VIR04_11265"
     CDS             4610..5605
                     /locus_tag="VIR04_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461367.1"
                     /translation="MKVLRLLIPALAALAMAGQAVAADKFKVGYLRVMDDAQAIVAYE
                     GGFYKNHGLDVDLVEFSSGTDLIKAIVGGQVDTGVLGFTNAVSWSSKGADLKVVSGAQ
                     MGFHALVVRDDAGVNKVADLKGHTLASQKEGSTADVVLKGVVLKDAGLKSTDLTIMGV
                     SPQVAVQSLVGKRVDAAFLFEPQARIAQLIAPVKQIYEIGEVWPFPCMVVITSNKSLQ
                     RNKEAVWKSLDAQRDAIDLLKNKPQAAAKLIAGYFIAEPTLKTLKHGDVPREDVIRDA
                     IKSQTFNAQLTPKELSRMQEIANIMQAQGSLKTRDGTAFQVKNIVDLDWQEARKL"
     gene            5719..6492
                     /locus_tag="VIR04_11270"
     CDS             5719..6492
                     /locus_tag="VIR04_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY9461368.1"
                     /translation="MSTQTRLTGYRKKLAMAVAVVAILLFWQIAAWFLPAFLMPGVPT
                     VLVRLWKEIPTAEFHLILSGSLTRLGVGYGVALLFGIGFGLIGGVLFFFREVLKSAII
                     ILQSIPSIAWVPLFLILMGFGNLPIIVVIALAAFFPAALSVMNATESVLRVHVSAARV
                     MGATHWDMLKRVYLPAVMPELITGAQLAFGNAWRALISAEMLIGFGQGLGRSLAYAGE
                     TADMVGVMANILTIAILATLIDQVILENLKRRLLHYQYV"
     gene            6507..7340
                     /locus_tag="VIR04_11275"
     CDS             6507..7340
                     /locus_tag="VIR04_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461369.1"
                     /translation="MSTRLSPLSVLFVLVALCGAGATMAFADAAGQRATQADGLVVGV
                     DYVVPPFVAGAKVRTPEAIDTTLAEQVAERMHVKLATVLAASTGRTDSEQPSKTGTAN
                     FVLAAVPLAAGNAGTASTVTIPTGYTAAPMAIMRSDTDIKTWEQLKGRIVCLSQGSRY
                     IGMAAARYGAVEKIFGAPADSLLALRTGRCDATVHDDTMLNELLKFPEWKKYSAQLPA
                     GPRVSLAFVVPAADSVTASLVKQMASQWKASRYLAGLTKTRARDIAFEVYLDQNVPDC
                     H"
BASE COUNT         1608 a         1922 c         2179 g         1713 t
ORIGIN      
        1 ccggtgtggc tggcgacacg gtattcgttg gcctaaccct ggatttgacg ggcccatttt
       61 cggcccaggg cgccgaggag aaacttggct atcaactggc tgccgagcag cttaacgcgg
      121 gggctcccga gattctgaaa atctcgccgc tgacaaaaaa aggggtgatg ggcaagaggg
      181 tcgagctggg tgttgctgat gccgaaacca agccgaatac cgcggtacag gcggctacgc
      241 attttttgca tgacaacaag gccatgttga tcaccggtag ttccagcagt gccgtggcga
      301 ttgctttgca aaaggtttgc ggccgcgaac atacgctcta catggcggcc atcagcgcct
      361 cgaacgatac gaccggcaag gactgccagc gttacggctt ccgcctgggc tttttcgcat
      421 accctgtttg caagatgatc gcgccggtgc tggccaggtc catgggcaaa aaccgcaagg
      481 cggcgtactt ggtgcccgac tacacctatg ggcacacggc gtttgattcg atgaaggagt
      541 tcaccgaaaa agagggctgg acgacggtca cggaacagct tcacccgctg ggcgccacgg
      601 actacggctc gtacctgatc aatattgcca atagcggcgc cgatacgctg gtcgtgctcg
      661 acttcggcgc cgatgcgtcc aattcgatca aacaggccaa acaattcggc ctgctcgata
      721 aaatgaagat tactgtgccc gtcatgtcgt cctttctgga aaaagagctt ggcgccgact
      781 tgatgcaagg cgtttatgcc gctacaggct tttggtggac actcgaggac aagtacccga
      841 ttgccaagga tttcgtgacg gctttcgaaa agaagtacaa cgccaaacca cgcgactcag
      901 cctatattgc ttatctgaat cttgcgttat gggccgacgc ggtggagcgc gcaaaaacct
      961 tttacccacc ggatgtgatc aaagccttcg aggccggtgt tgtgcgccaa ggcccggttg
     1021 gcgatgtcac tttccgcgcc caggaccatc agggaatcat caatttcccg attgtgcgcg
     1081 gcaagaagcc atccgacatg aagggtcccg atgatttctt cgagatcgtc cacgtcgttg
     1141 aaggcaagac agcgttgccc gaggtgggcc tgttgggctg caaacttggc gcttatgttt
     1201 gattggatgg agtcgtataa ctgaacatag tgaccggttg tttttctgtt gaccaaaagg
     1261 gctgccatgg cagcccttat caacaaacga ggtactatac ttttcaacga aactgttaag
     1321 gacttattcg catgatcacc tatccgtcgt ttaaagttgc ggcaatgcac gtgagcccgg
     1381 tatttctcga taccgagaaa accgtcgaca aggtgtgcgc gcttatagac gaggccgcat
     1441 cagccggcgc agaattgatc gtttttcccg aggcctttgt cccggctttt cctctgtgga
     1501 gttccgtcag ggcgcctatt ttcaatcatg actttttccg gcagcttgcc agtaacgcgg
     1561 tgcgtgtgcc ggggcccgag gtgaaacggg tcgcccaagc ggccaagcgg cataatgtca
     1621 cagtttcgtt gggcataaac gagggtacgg acgccagtgt tggttgtatt tggaacacca
     1681 acattatcat cggccccaat ggcgtcctgc taagccacca tcgcaaaatc gttccgactt
     1741 attttgaaaa actcacgtgg gcgaatggcg atggcgcggg cttgcgtgtg gtcaatactc
     1801 cgcttggcaa acttggtatg ctgatctgcg gcgagaatag caatccgctg gcgcggtttg
     1861 cgcttatggc gcaaggcgag caagttcaca ttgccaatta cccgcccttg tggccgaacg
     1921 gcggcaatta cgatattgtg cagtccatac gcgctcgtgc gcaggcgcac tctattgaag
     1981 ccaaggcctt taccgtcgtg tcttcggcat acttgacgga cgacgtaaaa gaattgatcg
     2041 ccgatggcga caaacggata cgcgaagtga tggacaatac cccacgttcg gcatcgatgg
     2101 tgatcggtcc caatggcgag caaatgggcg agatccgaca aaacgaagaa ggcatcattt
     2161 atgccgatat caacattgct gattgcgtgg ttcccaaaca gtttcatgat gtggtggggt
     2221 attacaatcg attcgacatc ttcaaactga cggtgaatcg gtcgtccaat gatcccatca
     2281 actttgacgc tgcagatcaa gaacgcggca ctgtcaacac cgaccttgcc gccttcggcg
     2341 aagcatcagc ttccgcggat tccaggtcac tggttggaca ggacataagt taattatcgg
     2401 ttataaaaat aagggggata tgtaatgagc ctgactctgt ctggtttaag agttctggac
     2461 ctgactcgag cgctggcggg gccgttctgt acgatggctc tggccgatct gggcgccgaa
     2521 gtggtcaagg cggaacccac gccgtcgggg gatatggctc ggcaatgggg tccgatcgac
     2581 cgggacatta gtgtctatta cttgagcgcc aaccggaaca aaaaaggaat cggaatcgat
     2641 tttcgaagcg ccgaaggctt ggcgctgctt aggcaactgg cgctcaaatc ggatatcgtg
     2701 gtcgaaaact tcaggaccgg caccatggaa aagatggggt tgggttatga gtcgctggcg
     2761 cgcgagaatc ccaagctcat tatggcgtcc atttcgggct tcggcaataa agggccggcc
     2821 aaggactggg ccgggttcga ccagatcgct cagggttact ccgggttcat gagcctgact
     2881 ggcacgccag agaccggacc cacgcgggtt ggcgttccca ttggagatct cacggcgggg
     2941 atgtggctta cgatcgggat cctggcggcc atcgtggaac gcggacggac gggccgcggt
     3001 cagcacgtgg agacctcttt attggccgga ctcatgagcc tgctttgtgt acagggtcag
     3061 cgttatctga atcttggcga ggttcccgag ccttgcggca acgctcaccc cgtgattgca
     3121 ccctacggca cttttgaaac cgccgacggc ccgttgaacc tggctccggc gacggaggcc
     3181 atgtgggtca aactgtgctc gcttctgggt gcaagccaat tggtcgacga ccctcgtttt
     3241 cttactaatg tagagcgccg aaaaaacgga gacgcgctca cgcaagaact tgaagcgcgg
     3301 ttgcgtgaaa agacccgcga agagtggacc gccatcatga tcgaaaatgg aattccggcg
     3361 ggtcccatca acagaatgga tgatgtcttc aacgacgcgc aggttgcggc ctgcaacctg
     3421 gtgcagactg ttgagcactc ggtattggga gccatcaaac tggtggcatt gcccgtacag
     3481 atgggatccg cgggcgatgc gcctacggtt cgttcggcgc ctccgctctt tggcgaacat
     3541 tcgcaaagca tacttgccga ttacggattt agctccgatg agatacaacg gctgcttgac
     3601 tccaaggtta ttcgccaggc aaaggcgggt gctgaaaagc aggacgcctg aagtatcgtt
     3661 tcgacgtgat gatccggcag cacgccgctg aagcctatat ttcttacagc tataaacata
     3721 gcacaataaa atgcttgtat ggaatgtagg ctgatcgtac agtgatactc atatatccag
     3781 atacatgagc gagagtgggg ccgtggctcc gctttttcgt taaagagggc tgaattctaa
     3841 tgaagctatc gttcaaaaat attcaaaaaa gctttgcggg gcttgatgtt atcgacaatt
     3901 tcagtcgtga aatgaatgat ggcgaattga ttgcgctgat cggtccctcc ggttgcggca
     3961 agtcgacttt gttgcacatt gctgcgggtc tggaaaaacc cactagcggt gtcctgctgg
     4021 ccgatggcgc ccccatcgcg gggcccgatc ctgaacgcac actcatgttt caggaaaatg
     4081 ctctttatcc atggctgact ttggcgcaga acgttgcgct ggcgcttgag tttcaaaaag
     4141 tcgacaagcg cgacgccaga gagcaggcgc ttgtatggct tgaaaaggtc aacctgaagg
     4201 gctttgctga ctactaccct catcaggtgt cgggcggaat gcggcaacgc gttgcactgg
     4261 ctcgcgcatt tatcacccgt ccgaaggtat tgttgctgga cgagcccttc ggggcgcttg
     4321 atgcattgac gcgaatgact ttgcaagacg ccttgcgcga gctgatacgc gaagcgcgcc
     4381 cgacggtcct gctggttacg cacgacgttg acgaggcctt gtttttggct gatcgcattc
     4441 tggtattcag ttcgcggccc gcgacagtat tgaaggaatt caatttggcg cgccgcgaaa
     4501 aaacacacga tttattggaa ttcgcggcca tgcgtcgcga gattttggag ctgctcggaa
     4561 ttcacgccaa acaggttgaa cttaccgggc tacaagagga aatggcggca tgaaagtact
     4621 acgtttatta attccggcgc tggcggcgct ggcgatggcc ggtcaggctg tggcggccga
     4681 caagttcaag gtcggctatc tgcgcgtcat ggacgatgcg caggcaatcg tggcttacga
     4741 aggcggtttt tacaaaaatc acggcctgga cgtcgatctg gtcgaattca gttcaggtac
     4801 cgacctgatc aaggccattg ttggcggtca ggtcgatacg ggcgttctgg gtttcaccaa
     4861 cgccgtgtca tggtcttcaa agggtgcgga tttaaaggtc gtcagcggcg cgcaaatggg
     4921 tttccacgcg cttgtcgtgc gcgatgatgc gggcgtgaac aaggttgccg acctgaaagg
     4981 tcatacgctt gcgtcgcaaa aagaaggcag tacggccgac gtggtgctaa aaggggttgt
     5041 actgaaagac gccgggctaa aatccaccga cttgacgatt atgggtgtca gccctcaagt
     5101 ggccgtccag tcattggtgg gcaagcgtgt cgatgccgcg ttcctgttcg agccgcaggc
     5161 gcgtattgct caattgattg caccggtaaa acagatttac gaaattggcg aagtatggcc
     5221 attcccatgt atggttgtca taacttccaa taaatctttg caacgcaata aagaagccgt
     5281 ttggaaatcg ctggatgcgc aacgcgacgc catcgacctg ctaaaaaaca agccgcaggc
     5341 ggctgccaag ctgatcgcgg gttatttcat tgccgagcca acgctcaaaa cattgaagca
     5401 tggcgacgtg ccgcgtgaag acgttattcg cgacgccatt aaaagccaga ctttcaacgc
     5461 gcagttgacg cccaaagagt tgtcgcggat gcaggaaatt gccaatatca tgcaggccca
     5521 gggctcgttg aagacccggg acgggacggc cttccaggtc aagaacatcg tcgatctgga
     5581 ttggcaggaa gctcgcaagc tataaaatga ggggcagagc ggccggcgcg ttgttcgcgc
     5641 cacatctatc gcacaactgc cgtttttgtt cccataaaaa tggccggtgt ttaccggccg
     5701 tcttgtttta gagcgcccat gagcacccag actcgtctta ccggttatcg caaaaaactt
     5761 gccatggctg tggcggttgt tgccatttta ttgttctggc agatagccgc gtggtttctg
     5821 cctgctttcc ttatgcctgg cgtgccgacg gtgcttgtgc gcttatggaa ggaaataccc
     5881 acggcggaat ttcaccttat cctgtctggc agccttaccc ggctgggcgt cggctacggt
     5941 gttgcgctgt tgttcggaat tggttttggt ctgatcggcg gcgtgttgtt tttcttccgc
     6001 gaagtcctga agtcggccat tattattttg cagtccatac cgtccattgc atgggtgccg
     6061 ctgtttttga ttctcatggg gtttggcaat ttgccgatca ttgtcgtgat tgcgctggct
     6121 gcgtttttcc cggctgcact atccgtcatg aatgccacgg aaagcgtttt gcgcgtccat
     6181 gtgtcggccg cgcgcgttat gggcgcaacg cattgggaca tgctcaagcg ggtctatttg
     6241 ccggccgtca tgccagagct gatcacgggc gctcaattgg cctttggcaa tgcctggcgc
     6301 gcgctgatct ccgccgaaat gctgattggc tttggacagg ggcttgggcg ttcgttggcg
     6361 tatgcgggcg agacggccga catggtgggg gtgatggcga atattcttac catcgctatt
     6421 ctggccactc tgattgacca ggttattctg gagaatctga agcgccgctt gttgcactat
     6481 caatacgttt gattgcagag accattatgt cgactcgatt atcgccattg tcagtcctgt
     6541 ttgttttagt cgcgctgtgc ggcgctggcg cgacaatggc gttcgccgac gcggctgggc
     6601 aacgcgccac gcaagcggac ggtctggtgg ttggcgttga ttatgtcgtg ccgcctttcg
     6661 tcgcgggcgc aaaagtgcgt acgccggagg cgatcgatac aaccctggcc gagcaagtcg
     6721 ccgaacgcat gcacgtaaaa cttgccaccg ttctggcggc ttccacgggg cgcaccgata
     6781 gtgaacaacc aagcaagacg ggtacggcca actttgttct ggcggcggtt ccgctggcgg
     6841 ccggcaatgc cggtacggca tcgacggtca cgataccgac aggttatacg gccgcgccca
     6901 tggcgatcat gcgcagcgac accgatatca agacgtggga acaacttaag gggcgtattg
     6961 tttgcctgtc gcaaggcagt cgctacatcg ggatggctgc cgcccgctat ggcgccgtcg
     7021 aaaaaatttt cggggctccc gccgattccc tgctggcgtt aagaaccggc cgctgcgacg
     7081 cgaccgtgca cgacgatacg atgctgaacg aattgcttaa atttcccgag tggaagaaat
     7141 actccgcaca gctcccggcg ggtccgcgcg tatctctggc gttcgtcgtg cccgccgccg
     7201 actccgtgac ggcatcgctg gtaaaacaaa tggcaagtca atggaaggca agccgctacc
     7261 tggccggttt gaccaaaacc agggcgcgcg atattgcttt tgaagtctac cttgatcaga
     7321 acgtgcccga ttgccactga ttgatcttag tgtggcaatt tccaccagct ttgaatgctg
     7381 ggacggctgg attgccgttc agtgtattgg cgaatattgg cc
//
LOCUS       DATCXU010000501         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_531225,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000501 DATCXU010000000
VERSION     DATCXU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531225"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1292
                     /gene="dnaJ"
                     /locus_tag="VIR04_11280"
     CDS             174..1292
                     /gene="dnaJ"
                     /locus_tag="VIR04_11280"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY9461370.1"
                     /translation="MAKRDFYEVLGVAKNATTAEIKKAYRKLAMKYHPDRNPDSKEAE
                     EKFKEAKQAYEALSDDQKRSAYDRFGHAGVDPNSGVGGMGDGGFADAFGDIFGEIFGG
                     GGRRGGGPQVYRGADLKYTLDISLEQAATGFDTEIRVPSWETCDVCHGSGAKPGTHAR
                     TCHTCGGSGAVRMQQGFFSVQQSCPTCHGTGKEITNPCVACDGVGRTRKNKTLQVKIP
                     AGIDDGMRIRSTGNGEPGVNGGPPGDLYVEIHLKPHGIFQRDGEDLHCELTIPFTRAA
                     LGGEIEVPTLVGKGEITIPEGTQTGKTFRLRGKGIRGIRASYPGDLYCHISVETPVRL
                     SDEQKKILHEFEESLLAGGEKHSPKSESWTDRVKSFFS"
     gene            complement(1310..4012)
                     /locus_tag="VIR04_11285"
     CDS             complement(1310..4012)
                     /locus_tag="VIR04_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY9461371.1"
                     /translation="MGAPASALANDAKIPDGVTQNSSVEGVTQYTLENGLRILLAPDD
                     SKPSTMVNMTYLVGSRNENYGQTGMAHLLEHMLFRGTPTLHDALAEFSRRGLRANGTT
                     SSDRTAFYASFASNAETLDWFIGWQADAMVNSMIAKQDLDSEMTVVRNEMERGENNPF
                     QMLMQKMQAAAYQWHNYGHSTIGARSDVENVDVKQLRAFYKEYYQPDNAVLIISGKFD
                     PETTLNTIAAVFAKIPKPTRTLPVEYTVEPVQDGERQITLRRHGGSPLAAALFHIPAL
                     ADPDYTPLDLGVSILSDTPSGRLYHALVEKKLATDVFGFTAGLRQPGYALFGAQLEPG
                     MDQKLTLKTLDDTIASIATQPFTQAELDRIKNKWLTDWDRTYSNPISLAGALSDAVSD
                     GDWRLFFLQRDRVKAVHLADVQRATQAYLISSNRTNGLYIPTDKPVRAPAAGQPDLAA
                     LFKDYHGKTAPKEVAAFDPTPANIDAATQRAPLVLPNGEVKLALLPKPTRGDRVQARL
                     LIQFGAADNLKGYRSIAKATASLLDHGTSTLSRQEIEDKIDALQAEVDIGGDGGDVAV
                     DMTSTREHLPDLIALVLDIVRNANFPADELAKYQRQINTSIKNSMDEPAALASRTLAR
                     HGNPWPSDDIRYTPTFEQALHNVNALTRQELVDFHQKFYGAGHVEFAAVGAFDPAAVK
                     AALSSGLKGWRTAPAYERPSDPYHAITPRQFTINTPDKANAFYLAALPIKVQDTDPAY
                     PALYLANYLLGSSETSRLWNRIRVQDGLSYDVRSHLNVSSFEPSGAWTIYAIHAPQNS
                     QRLQQDIQQELSRALRDGFTDKEVSEGIHALLNYRKLARAQDGTLAATWINYLQLGRS
                     FAWSEKIDRELAALTPEKVNAALRATLTPDQFSSALAADSNKEK"
     gene            complement(4120..5289)
                     /locus_tag="VIR04_11290"
     CDS             complement(4120..5289)
                     /locus_tag="VIR04_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY9461372.1"
                     /translation="MLTDFFYCLRAHKLPVSVQEYLTLLSALQSDIMVPTLDSFYYLA
                     RTMLIKDETLFDRYDQAFGQFYRQIEASIGEGEIPADWLLKTIVKNLSKEEKAAIEKH
                     GWEKLMALFKKRLEEQKERHAGGNKWIGTGGSSAFGHAGYHPEGIRVGGPSAGNRTAV
                     KVWEQREYRDYDDQQQLGTRNFKIALRRLRRFAREGAELELDLEDTISHTAKNAGFLE
                     LQMAPERRNAVKVLMLLDVGGSMDDHVARVEELFSAARSEFRHLQVYYFHNCPYESLW
                     QSNQRRTREHVDTQEILRTYNDDWRLIIVGDATMSPHEVLQIGGSVEHYNKQAGATWL
                     QRLLDNWPKSVWLNPEPEGYWPYRQSIAIIKTIMHDQMYGLTAAGLERAMRHLSR"
BASE COUNT         1101 a         1434 c         1618 g         1242 t
ORIGIN      
        1 gccggcgcag gcggacgaaa atgtggtgga cgccgatttt aaagaagtta agcgcgacaa
       61 ataataggct gccgtagtgg tctggaaccc gatttccggt atttcgccgg aagtcgggta
      121 ttcttgtccc ggcccgaaaa atgggcgtta tggacacaac aaattgaatt gacatggcaa
      181 aacgcgattt ttacgaagta ctgggcgtcg caaagaacgc cactacagcc gagataaaaa
      241 aggcttaccg caagctggcg atgaaatacc atccggatcg caatccagat agcaaagagg
      301 ccgaagaaaa attcaaagag gccaagcagg cttatgaagc gttatccgat gaccagaagc
      361 ggtctgccta tgatcgtttt ggccatgcgg gcgtcgatcc caattctggt gtcggcggca
      421 tgggcgacgg cggttttgcc gatgcctttg gcgacatttt cggcgaaatt ttcggcggtg
      481 gcggccggcg cggcggcggc ccgcaggttt atcgcggcgc cgatttgaaa tacactctgg
      541 atatcagtct ggagcaggcg gcaaccggct tcgataccga gatccgggta cccagttggg
      601 aaacatgcga cgtgtgtcat ggttctgggg ccaagccggg cacacatgcc cgaacctgtc
      661 atacctgcgg cggcagcggc gcggtacgca tgcagcaagg ctttttcagc gtgcagcaat
      721 cttgtcccac ctgccatggc acgggaaagg aaatcaccaa tccttgcgtt gcctgcgatg
      781 gcgttggcag aacgcgcaag aataaaacac tccaggtcaa aatcccggca ggcattgatg
      841 acggcatgcg catccgctcg accggcaacg gtgagccggg ggtcaacgga ggccccccgg
      901 gagacttgta cgttgaaatt catttaaagc cgcacggaat attccagcgc gatggcgaag
      961 acttgcattg cgaactgacc attccgttca cgcgggcggc gttgggcggg gaaatcgaag
     1021 tgccgactct ggtcgggaaa ggtgagatta ccattccgga aggcacgcaa acaggcaaga
     1081 cctttcgcct gcgcggcaag gggatacgcg gcatccgggc cagctacccc ggcgatttgt
     1141 attgccatat ctcggtggaa acgccggtcc gcctgtctga tgagcagaag aaaattttgc
     1201 acgagttcga ggaatcgctt ttggccggag gcgaaaaaca ttcgcctaag agcgaatcct
     1261 ggaccgatcg ggtcaagagt ttttttagct gacccccaac ccgctacgct tacttttcct
     1321 tgttgctatc ggcggcaagc gcgctgctga attgatcagg cgtaagggtg gcgcgcagtg
     1381 ccgcattcac tttttcggga gttaatgcgg ccagttcccg atcgattttt tccgaccagg
     1441 cgaagctgcg ccccaactgc aggtagttaa tccacgtggc ggccagcgtg ccatcctggg
     1501 cgcgcgccag cttgcggtaa ttcaacaagg catgtattcc ttcgctgact tctttgtcgg
     1561 tgaaaccatc acgcaatgcg cgcgagagtt cttgctgtat gtcctgttgc agacgctggc
     1621 tgttttgcgg cgcgtgaatg gcgtagatcg tccaggcgcc ggacggttcg aaggaagata
     1681 cattcaggtg gctgcgaacg tcgtacgaaa gtccgtcctg cacacggatc cggttccata
     1741 agcgcgaggt ttccgaactg cccagcaaat agttagccag atacagggcg gggtacgctg
     1801 gatcggtatc ctgcactttt atcggcagcg ccgccagata aaaggcattg gctttgtctg
     1861 gcgtattgat cgtgaactgc ctgggagtga tcgcgtggta cggatctgac gggcgttcat
     1921 aagcgggggc ggttcgccag cccttaagac cggagcttag cgcggctttg acggcagccg
     1981 ggtcgaaggc cccaaccgcg gcgaactcca cgtgtccggc gccgtaaaat ttttgatgga
     2041 aatccacaag ttcctgacgg gtgagggcat ttacattatg cagcgcctgt tcgaacgtgg
     2101 gcgtgtagcg aatgtcgtcg ctgggccatg ggttgccgtg acgcgccagt gttcgcgatg
     2161 ccagcgcggc gggttcatcc atggaatttt tgatcgaagt attgatctgg cgctggtact
     2221 tggccagttc gtcggcgggg aaattggcat tgcgcacgat gtccagaacc aacgcgatca
     2281 ggtcagggag gtgctcgcgc gtgctggtca tgtcgacggc gacatcgccg ccatcgccgc
     2341 cgatgtcgac ctctgcctgg agagcgtcga ttttgtcttc gatttcctgg cgggatagtg
     2401 ttgatgttcc atggtcaagc agcgaagccg tggccttggc gatgctgcgg tatcccttca
     2461 aattgtcagc ggcgccgaac tgaatcagca gtctggcctg aactcggtcg ccacgggttg
     2521 gcttgggcaa caacgccagc ttgacttcgc cgttgggcag cacgagtggc gcacgttgtg
     2581 tcgccgcgtc tatgtttgcc ggagttgggt cgaaggcggc cacttctttc ggcgccgttt
     2641 tgccgtgata gtctttgaaa agggcggcca ggtccggttg tccggcagcg ggggcgcgca
     2701 ccggtttatc agttggaatg tacaaaccgt tcgtgcggtt gctgcttatc aggtaggcct
     2761 gcgttgcgcg ctgaacatcg gccaggtgca ccgccttgac ccgatcgcgt tgcaggaaga
     2821 acaagcgcca gtcgccatcg gacacggcgt cggataaggc tccggccagg ctgatggggt
     2881 tggaataagt tcggtcccag tcggtgagcc atttgttttt tatccggtca agttcggcct
     2941 gcgtgaaggg ctgcgttgcg atagatgcta tcgtgtcgtc aagtgtcttc agggtcagct
     3001 tttggtccat gccgggttca agttgtgcgc caaaaagcgc gtaccccggt tggcgcaagc
     3061 cggctgtaaa gccaaatacg tccgtggcga gctttttttc gaccagtgcg tgataaagcc
     3121 gtcccgacgg tgtgtcgcta agtatggaaa cacccagatc cagcggggtg taatcgggat
     3181 cagccagcgc gggtatgtga aacagcgcgg cagccagggg gctgcccccg tggcggcgca
     3241 gggttatttg ccgttcaccg tcttgcactg gctcgacagt atattcaacc ggaagagttc
     3301 ttgtcggttt gggaattttt gcaaagaccg ccgcaatggt gttcaacgtt gtttcggggt
     3361 cgaattttcc tgaaataatc aagacggcat tatcgggttg gtaatattcc ttgtaaaagg
     3421 cccgaagctg cttcacatcg acgttctcga catcggatcg ggcgcctatg gtgctgtgac
     3481 catagttgtg ccactgatag gcagcggcct gcatcttctg catcagcatc tgaaacggat
     3541 tgttttcacc gcgctccatt tcgttgcgca ccacggtcat ctcggaatcc aggtcctgct
     3601 tggcgatcat cgaattcacc attgcatcgg cctgccagcc tatgaaccag tccagtgtct
     3661 cagcatttga ggcaaaactt gcatagaagg cggtgcgatc gcttgatgtg gttccgttgg
     3721 cgcgcaggcc gcgccgggag aattccgcca aagcgtcatg cagcgtgggg gtgccgcgaa
     3781 acaacatgtg ttccagcaaa tgcgccatgc cggtctgacc gtaattttca ttgcgcgacc
     3841 cgaccaggta cgtcatgttg accatggtgg agggctttga atcatcgggc gccagcagaa
     3901 tgcgcaagcc attttccagt gtgtattgcg tcaccccctc gaccgaacta ttttgcgtaa
     3961 cgccgtccgg aatcttggcg tcgtttgcga gggcactggc gggcgcgccc atcagggcag
     4021 cgatgaacac gcttaagcag gcgattgttc tggcgaaaag agagtgcatg cgttcatatc
     4081 cgaaaatgat tggaggctgc gccacccgat aggttcaatt caacgcgaca ggtggcgcat
     4141 ggcccgttcg agtcccgcgg ccgtcaggcc gtacatttgg tcgtgcataa tggttttgat
     4201 aatagcgatg gattgccgat aaggccagta gccctcgggt tccggattaa gccagaccga
     4261 tttaggccag ttgtcgagca gacgttgcaa ccaggttgct cccgcctgtt tgttgtagtg
     4321 ttcgaccgat ccgccaattt gcaggacttc atgcgggctc atggtggcgt cgcccacaat
     4381 aatcaagcgc cagtcgtcgt tgtaagtccg cagaatttct tgcgtatcga cgtgttcgcg
     4441 ggtgcgccgc tggttgctct gccatagcga ttcgtaagga cagttatgaa agtagtacac
     4501 ctgaaggtgg cgaaattcgc tgcgcgcggc ggaaaaaagc tcttcgacgc gggcgacatg
     4561 gtcatccatg ctgccgccga catccagcaa cattagcact ttaacggcgt tacgccgttc
     4621 aggcgccatt tgaagttcaa gaaagccggc gttttttgcg gtatgactga tggtgtcttc
     4681 cagatcgagc tccagctccg cgccctcgcg cgcgaaacgc cgcaggcgcc gcaatgcgat
     4741 tttgaaattg cgcgtaccca gttgctgctg atcatcgtaa tcgcggtatt cccgttgttc
     4801 ccataccttt acggctgtgc gattgcccgc cgatgggccg ccgacgcgaa tgccttcggg
     4861 gtgatatccg gcatgcccga aggccgacga gccgcccgtg ccgatccatt tgttgcctcc
     4921 ggcgtgccgc tccttttgtt cttccagcct tttcttgaat agcgccatca gcttttccca
     4981 gccatgcttt tcgatagcgg ccttttcttc tttgctcaga tttttcacga tcgttttcag
     5041 gagccagtcg gccggaattt ctccttcgcc tatggatgcc tcgatctgcc tgtaaaactg
     5101 gccgaaggcc tgatcgtaac gatcgaagag cgtttcatcc ttaatcagca ttgtgcgcgc
     5161 cagataataa aagctatcca gcgtaggcac catgatatct gactgcagag cgcttaaaag
     5221 cgtaaggtat tcctgcaccg agaccggcaa tttatgcgct cgcagacagt aaaaaaagtc
     5281 agtcagcatg atctggcgta atgagattta gcggtgctgt ccggcgcgcg tcatcattgc
     5341 caggcgttgc agcagtgaca ggtcctgttc gttcttgagc agggcgcccg ccatt
//
LOCUS       DATCXU010000502         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_613997,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000502 DATCXU010000000
VERSION     DATCXU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613997"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VIR04_11295"
     CDS             complement(<1..950)
                     /locus_tag="VIR04_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY9461373.1"
                     /translation="MMAVKKENALIVTLAAAGILMITMGTRQSFGLFISPVNSSTGMG
                     IVAISFALAIGQIAWGAIQPIAGACADHYGPRGVLIAGIVVLALGCAITPFMGTGFSL
                     MVSLGLLASIGSGAGSFSVLIGAAAQQLPAKSQGVASGVINAGGSFGQFVFAPVMQKL
                     IQSVGWMGAMWSMAALTLAALPLIGTLTKGYKTQHTHVKTDQGLRETVSASLRNRSYL
                     LLNIGFFTCGFHIAFLVTHLPGEIDLCGLPPAVAGWSLAIIGLANIFGSLIFGACVDK
                     YRSKYILALMYASRALLIAWYLAMPRVEWTFYLFAAGLGFT"
     gene            complement(947..2506)
                     /locus_tag="VIR04_11300"
     CDS             complement(947..2506)
                     /locus_tag="VIR04_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY9461374.1"
                     /translation="MSIRKHAAFYIPAIFLWLTLIAGCATAPSLQNRIPSHALAASES
                     AATILGKADARLSAAHPGKTGIHTLPNPREAFAVRALLARAAQRTLDVQYYLWQKDLT
                     GTLLFESLHAAAERGVRVRLLLDDLNTSGLDPTLAALDSDPNIEVRLFNPFFLRSPRW
                     VNYISDFSRLNRRMHNKSFTADNQVTIIGGRNIGDEYFGVAGDVSFTDLDVLAIGPVV
                     KDVSRSFDDYWNSQSAYPASLLMPLGTPAELQKLTDEASALELEPRASAYVQALRGSS
                     LLHQLADGTIPLEWATTHMLSDDPRKGLGQVPAKELLSYKLERAIGKPRSHVELISSY
                     FVPTAAGVTDLVALAKRGVKVKVLTNSLEATDVAAVHAGYAKRRKPLLAAGVKLYELR
                     RQSPAGKKDRDEGHFRSSGSSLHAKTISIDGSRVFIGSFNFDPRSAKLNTELGFVIDS
                     PELAQAIAKSFDNNVPDTAYEVHLSETGDLYWTEIKDGKEIRHNAEPGASLWRRAVVG
                     FLSLLPIDWLL"
     gene            2604..3452
                     /locus_tag="VIR04_11305"
     CDS             2604..3452
                     /locus_tag="VIR04_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY9461375.1"
                     /translation="MFRWLSGRGATQRQVERELAQITPSTWQTAVARYRFLAGLSAEE
                     DEALRQRAAWVLASKTFVGVQGLEVTGKIMLSIAIQAALPILKLDPVLYEGWIEIVLY
                     PGGFLVPRTDVDESGVVHEYVEATSGEAWDGGPVILSWEDSRHDKSRKSNLVIHEFAH
                     KLDMHYGEADGMPSLAAHAELSPRQWRHVLDVSFKHFNNQLRAVEDAIPRHVDPDSDD
                     AAPWFDTLPMDPYAGANTAEFFAVSSEAFFVNPEPLARILPEWYGLLSRYYRQDPLRR
                     LARHPY"
     gene            complement(3449..4396)
                     /locus_tag="VIR04_11310"
     CDS             complement(3449..4396)
                     /locus_tag="VIR04_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461376.1"
                     /translation="MNIIHLIMKMNLPLKSIQAFEVAARLGSFAAAATELNLTPSAVS
                     HQVRFLEERLSVTLFHRVHRGVVLTDVGRRYAQAVARGLGMIDTATRSLERDGKHDVL
                     TIHCAPSLAAQWLMPRLSRFSALYPNIGIRLNASVAAIDLAAEAADFDIRYGPVFSDP
                     DIIAASFPKESLAVVCAPSLLVNGHAIDAPSDVQFHTLIHSEVNLLSWREWARQHDVA
                     DLDLKQGLSFDRSFMSISAVIDGLGIALESVLLVERELASGKLVLPLGADPALLTFHR
                     LLYLQSKAHLPKMVAFKDWLFEQLDESFKGLKSAGGKSG"
     gene            4501..5256
                     /locus_tag="VIR04_11315"
     CDS             4501..5256
                     /locus_tag="VIR04_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9461377.1"
                     /translation="MQGLLNGKVAVITGAASLNGIGRRTAHVFAEHGASVVIVDLDKN
                     ASVAAAESLGAGHLGLAGNVSSREDCQNAIDAVLARYGQVDVLINNAGITQPVKVMDI
                     SGDDYQRITDVSLRGTLYMSQAVIPSMRDKRAGSIVCMSSVSAQRGGGIFGGPHYSAA
                     KAGVLGLARAMARELGPDGIRVNSIAPGLIQTDITAGKLTDAMKADIIKGIPLSRLGE
                     AKDVANCCLFLASELSGYLTGVTLDVNGGMLIH"
     gene            5267..>6085
                     /locus_tag="VIR04_11320"
     CDS             5267..>6085
                     /locus_tag="VIR04_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY9461378.1"
                     /translation="MTKEQLVALAHRAYRIRRYALQMGEVQGQGYVGQALGYADVLAT
                     AYLHAMTIRPGDPKWEGRDRFLLSHGHYAIALYAALIEAGIINAEELDTYGSDDSRLP
                     MSGMASYTPGMEISGGSLGQGLSIAVGMALALRQKGGKQFVYNSMSDGELDEGATWEA
                     AMSAGHHGLSNLICLVDINNQQADDNSNKVLGFEPLADKWAAFGWYAQRVNGNDLAAV
                     VQAFDNARQCDQAKPRVILFDTLLGKGVAFLEQREKAHFIRVDAAEWQQAIAILD"
BASE COUNT         1332 a         1633 c         1807 g         1313 t
ORIGIN      
        1 gtaaaaccca ggccggcggc aaataggtaa aacgtccatt cgacgcgcgg catggccagg
       61 taccaggcaa tcaacaaagc gcgcgaggca tacatgagcg ccaatatata tttgctgcgg
      121 tacttgtcca cgcaagcccc gaaaatcagg ctgccgaata tattggccaa acctatgatc
      181 gccagagacc agccggccac agccggcggc aaaccgcaca aatcgatttc gccgggcaaa
      241 tgcgtaacca ggaacgcaat atgaaagcca caggtgaaaa agccgatatt cagcagcaaa
      301 tagctgcgat ttctcagtga agcactgact gtttctctaa gcccctgatc ggtctttaca
      361 tgtgtgtgct gtgtcttata acctttggtc aaggtgccga taagtggcaa ggcggccagc
      421 gttagcgccg ccatggacca catcgccccc atccagccca ccgactggat cagtttttgc
      481 atgaccggtg cgaatacaaa ctgtccgaac gacccgcctg cgttgataac gcccgaggcg
      541 acgccctgcg atttcgccgg caattgctgc gccgccgcgc cgatgagcac cgagaagctg
      601 cctgcacccg agccgataga cgccagcaag cccaacgaca ccatcaagct gaacccggtt
      661 cccatgaacg gcgttatcgc gcaccccaac gccaggacca cgataccggc aatcaggaca
      721 ccacgcggcc catagtgatc ggcgcaagcc cccgcaatgg gttgaatggc gccccaggcg
      781 atttgtccga tggccagtgc gaagctaatg gccacgatac ccatgccggt ggaactgttg
      841 accgggctga tgaacaggcc gaacgactgt cgcgttccca tggtgatcat cagaatcccg
      901 gcggcggcca gggtcacaat cagtgcgttt tcttttttaa cggccatcat agcaaccaat
      961 caataggcaa tagcgacagg aaacccacca cggcgcgccg ccatagactg gcgcccggct
     1021 ctgcattgtg acgaatttct ttaccatcct tgatctccgt ccaatataga tcgccagttt
     1081 ctgatagatg cacttcataa gcggtgtcag gcacattatt gtcgaagctc tttgcgattg
     1141 cctgcgccag ttcggggctg tcaatgacga aacccagttc ggtattcaat ttggctgaac
     1201 gtggatcaaa gttgaatgaa ccgataaaga cacgcgagcc gtcgatggaa attgtttttg
     1261 catgaaggct ggaaccggag ctgcggaaat ggccttcgtc ccggtctttc ttgcccgcgg
     1321 gggactggcg tcgcaattcg taaagtttta cgcctgcggc aagcagtggt ttgcgtcgct
     1381 ttgcatagcc cgcatggacg gctgcaacat cggtggcttc cagtgagttg gtgagcacct
     1441 tcactttaac tccgcgtttt gccagtgcaa caaggtcagt gacgccggcg gcggtgggta
     1501 cgaaatagga agagatcagc tcgacatggg atcggggttt gccgatggcc ctctcgagtt
     1561 tatagctcaa taattccttg gccggaacct ggcccaggcc tttgcgaggg tcgtcgctga
     1621 gcatgtgcgt ggtggcccac tccagcggaa tggttccgtc cgccaattga tgcaatagac
     1681 tggagccgcg caatgcctgc acataagcgc tggcgcgggg ctcgagctcc agggccgaag
     1741 cctcgtctgt cagcttctgc agttcagcgg gtgtgcccag gggcatgagc aggcttgccg
     1801 ggtaagctga ctggctgttc cagtaatcgt caaaactgcg cgacacatct ttgaccacgg
     1861 gtccgatcgc aagcacatcc agatcggtaa acgacacatc gcccgccacc ccgaaatatt
     1921 catcccctat gttgcgcccg ccgatgatgg tgacttgatt atcggcggta aatgacttgt
     1981 tatgcatgcg acggttcagg cgggaaaagt cgcttatgta atttacccag cggggcgatc
     2041 gcaaaaaaaa gggattgaat aagcgcactt cgatattcgg atccgaatca agtgcggcta
     2101 atgttggatc cagtcctgac gtattgagat catcgagcaa caagcgcaca cgcacaccgc
     2161 gttccgcagc agcgtgtagc gattcgaaca gcaacgtgcc ggtcaggtct ttttgccaga
     2221 ggtaatactg aacgtcaagc gtgcgttgcg cagcgcgcgc caggagcgct cggacggcaa
     2281 aggcttcacg cggattgggc aatgtgtgga tgcccgtctt gccggggtgg gccgcactaa
     2341 gccgggcgtc tgctttgccc agaatggtgg ctgcggattc gctggccgca agggcgtgcg
     2401 atggaatccg gttttgcagc gagggcgctg tggcgcaccc ggcgatcagc gttaaccaca
     2461 gaaaaatggc aggaatataa aacgcggcat gttttcgtat gctcattgtt caagattcct
     2521 gtgggtcgct ggcccgtaag cttaaagcac ttcggaggcc gggggaatag tatagtgtct
     2581 ttatactaac tggaccgacg cccatgttca gatggcttag cggccgcggc gccacgcaac
     2641 gccaggtgga gcgtgagctg gcccagatca ccccttcaac ctggcaaacc gcggttgcgc
     2701 ggtatcgatt tttggccggc ctgtccgcgg aagaagacga agccttgcgt cagcgcgcgg
     2761 catgggtgct tgccagcaaa acctttgtcg gcgtacaggg cctggaagtg accggcaaga
     2821 tcatgctttc cattgcgatt caggcggctt tgcccatcct taaattggac cctgtgctct
     2881 atgaaggctg gattgaaatt gtgctgtatc ctggcggctt tctcgtgccc cgcaccgatg
     2941 tcgatgaaag cggcgtagtg cacgaatatg tggaagcgac ctccggcgaa gcatgggacg
     3001 gcggtccggt cattctatcg tgggaggata gccgtcacga caagtcgcga aagtcaaacc
     3061 tggtcatcca cgaatttgcc cacaagctgg acatgcacta tggcgaggcc gacggtatgc
     3121 caagcctggc cgctcatgcc gaattgtcgc cccggcaatg gcggcatgtt cttgatgtct
     3181 cgtttaaaca cttcaacaat cagttgaggg ctgtggaaga cgcgattcca cggcatgttg
     3241 atcctgattc cgacgatgcc gcgccttggt tcgataccct gccaatggat ccgtatgccg
     3301 gcgccaatac agcggaattt ttcgcggtga gttcggaagc gttctttgtg aatccggagc
     3361 cgctggccag gatactgcct gaatggtatg gcttgttgtc gcggtattac cggcaagatc
     3421 cgttacggcg ccttgcccgc catccgtatt agccactctt gccgccagcg cttttcaatc
     3481 ctttgaacga ttcgtccaat tgttcgaaaa gccagtcctt gaacgcaacc atcttcggta
     3541 aatgcgcttt tgactgcaag tagagcaaac gatggaaggt tagcagggcg gggtcggcgc
     3601 ccagcggtaa aaccagtttg ccgcttgcca gttcgcgctc cacaagcaga acgctttcca
     3661 gcgcgattcc caggccgtca atgaccgcac taatggacat gaacgagcgg tcgaagctta
     3721 ggccttgttt caaatccaga tcggcaacat cgtgttgtct ggcccactcg cgccaggaca
     3781 gcaagttaac ttcagagtgg ataagcgtgt ggaattgcac atccgagggg gcgtcgatgg
     3841 cgtggccatt gacgagcagg gagggcgcgc acacgacggc cagggactcc ttggggaagg
     3901 atgccgcaat gatgtcgggg tccgaaaaaa cgggtccata gcggatatcg aagtccgcgg
     3961 cctcggcggc cagatcgatg gcggccactg aagcgttaag ccgtatgcca atatttgggt
     4021 acagcgcgct gaagcgcgat agtctgggca tgagccattg cgcggccagg ctgggtgcgc
     4081 agtgtatggt cagtacatca tgtttgccgt cacgctcaag actgcgcgtc gcggtgtcga
     4141 tcatgcccag cccgcgcgcc accgcttgcg cgtagcgcct tcccacgtca gtcagtacta
     4201 caccgcgatg cacgcggtgg aagagtgtga cgcttaatct ttcttccaga aaacgcacct
     4261 gatggcttac ggccgagggg gtcaagttca gttccgtcgc tgcagcggca aacgacccca
     4321 gtcgcgctgc cacttcgaag gcctggatgg atttgagcgg gaggttcatc ttcataatca
     4381 ggtgaattat attcacttat catggcgttt tattcgtttg cgcctcggtt gtgcagttac
     4441 tagaattcac caataaacct tcgcatccgc gtggtgcgcc ataaattcgg gagcaggaaa
     4501 atgcaagggc tattgaatgg aaaagttgcc gtcattacgg gagcggcctc tttgaacggt
     4561 atcggccggc gcacggcgca tgtattcgcc gagcatggcg catcggtcgt catcgtcgat
     4621 ctggacaaga atgcctcggt tgccgcagcg gaaagtttag gggccggcca tctcgggctg
     4681 gcaggcaatg taagcagtcg cgaagattgc cagaatgcca ttgacgccgt gctggcgcgt
     4741 tatggacaag tggatgtcct gatcaataac gccgggatta cacaacccgt caaggtcatg
     4801 gacatttccg gtgacgacta tcagcgcatt acggatgtgt cattgcgtgg cacgctgtat
     4861 atgagccagg cggtcattcc gtcaatgcgg gataaacgcg ccgggtccat cgtttgtatg
     4921 tcgtcggtgt cggcccagcg tggcggcggg atctttggcg ggccgcatta ttcggccgcc
     4981 aaggcgggtg tgctggggct ggcccgcgcc atggccaggg agcttgggcc ggatggaatt
     5041 cgcgtcaact cgattgcccc tggattaatt cagaccgata tcaccgctgg caagctgacc
     5101 gacgctatga aagccgacat tatcaaaggc attcccctga gccgtctggg cgaagcaaag
     5161 gacgttgcca actgttgttt gtttctggcg agcgaattat ctggctacct tacgggcgtc
     5221 acgctggatg tcaatggcgg catgcttatt cattaatggg ttaattatga ctaaagaaca
     5281 gttagtcgcg ctcgcgcacc gtgcatacag gattcggcgt tatgccctgc agatgggcga
     5341 ggttcaaggg caggggtacg ttggacaggc tttgggctac gccgatgtat tggcgacagc
     5401 gtatctgcac gccatgacga tcaggcctgg cgatccgaaa tgggagggcc gcgacagatt
     5461 cttgctgtcg catgggcatt acgccattgc gttatatgcg gcgctgatcg aggcaggcat
     5521 tatcaatgcc gaagaattgg atacgtatgg ttcggacgat agccgcttgc caatgtccgg
     5581 catggcgagc tatacgccgg gtatggagat ttcgggcggc tcgcttgggc aagggttgag
     5641 cattgccgtg ggtatggcat tggctctaag gcaaaagggc ggcaagcagt ttgtatataa
     5701 ctccatgtcg gacggcgaac tcgacgaagg cgccacatgg gaggccgcga tgtcggcggg
     5761 ccaccacggt ttgtccaatt tgatttgtct ggtggatatc aataatcagc aggcggacga
     5821 caattcgaac aaagtattgg gtttcgagcc tttggccgac aaatgggcgg catttggctg
     5881 gtatgcgcag cgcgtcaatg gcaatgatct ggccgcggtc gttcaggcct tcgataatgc
     5941 gcggcagtgc gaccaggcta aacccagggt cattttattc gacacattat tgggtaaagg
     6001 cgttgcgttc ctggagcaac gggaaaaagc ccactttatc cgcgtcgacg ccgcagagtg
     6061 gcagcaagcc atcgcaattc tggat
//
LOCUS       DATCXU010000503         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_364210,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000503 DATCXU010000000
VERSION     DATCXU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364210"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VIR04_11325"
     CDS             <1..369
                     /locus_tag="VIR04_11325"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEY9461379.1"
                     /translation="ELFGELFGDAIGDLGVIDTHSQRNALLDQLQQHPAGKLLLVCDA
                     RQTPDRGAIALVAELARYAHQTHIVLSTGIEHDSRADLRPGLWQQRLAQAGFAPEAIH
                     SQLTDALAWLKNDKAEHGRH"
     gene            365..1753
                     /locus_tag="VIR04_11330"
     CDS             365..1753
                     /locus_tag="VIR04_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEY9461380.1"
                     /translation="MKRAPLRLAVVGHTNTGKTSLLRTLTRDPGFGQVEDAPGTTRHV
                     EGIRLLVDGQAAVEFFDTPGMEDGIALLDYLNQLAQRGERPDGPERIRRFLDSPESRR
                     RFEQEARVVGKLLDCNAGLYVVDVRDPVLGKHKDELTVLASCGRPLLPILNFIHGAEG
                     RTAAWRDALARLGLHVVVEFDTIAPELDGETQLYDKLALLLDAYAPLLHLLKADIARQ
                     RDIRHADAARLIAELLADAAAWRLTSEPGAQAVTLASETLREKIRAREDQCVRALLKR
                     YNFHIHDFPAHSLPLKGERWGMDLFHPQALKDVGIHVTKGLAAGAMAGAAIDAFTAGL
                     SLGSATLIGATAGGLWQGIDRLGKRVLGRLKGYRELSVDNNVLELLALRQIHLLQALE
                     RRGHAAQQPIVLTSGTALEGLRNARIMIELDEIRSQPSWSTLTDEYTNSARRESVLRS
                     LTHKLEKWSLTT"
     gene            complement(1743..2228)
                     /locus_tag="VIR04_11335"
     CDS             complement(1743..2228)
                     /locus_tag="VIR04_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY9461381.1"
                     /translation="MKEITGWATVQGHWLRRKHFTPRVLAGIAEQIRKGELDHAGELM
                     VAVEAVSPIHERDSHLRALEVFGRLRVWDTHHNTGVLLYLALDRQRIELIADRGVGAP
                     NALWEQICDQLRARLQRGDYAAGVTAAVDAIEQALRTHCPPLADGELNSNDLPDRPVM
                     L"
BASE COUNT          491 a          715 c          671 g          427 t
ORIGIN      
        1 gagctatttg gcgagctatt tggcgacgcc attggcgact tgggcgtgat tgacacgcac
       61 agccagcgca atgcgttgct tgaccaactg cagcaacacc cggcgggaaa attgctgctc
      121 gtatgcgacg ctcgccaaac acctgaccgc ggggccatcg ccctggtggc ggagctggcc
      181 cgctacgcgc accaaaccca tattgtcctg tcaacgggta tcgaacacga tagccgggcg
      241 gacttgcgcc cgggtctttg gcaacaacgt cttgcgcaag ccgggtttgc gcccgaagcc
      301 atccactcgc aactgaccga cgcgctggcg tggttaaaaa acgataaggc cgaacatggc
      361 cgacattaag cgcgcacctc tgcgcctggc cgttgtcgga cacaccaaca ccggcaaaac
      421 ctcgttgttg cgcacgctga cccgcgatcc gggatttggc caggtcgaag acgccccagg
      481 caccacacgc catgtcgagg gcatacgtct gctggtggat ggccaggccg ccgtcgaatt
      541 tttcgacact cccgggatgg aagacggcat cgcgctactg gactatctga atcaactggc
      601 acagcgtggc gaacgaccgg acggacccga gcgcatccgg cgttttctgg attcgccgga
      661 aagccggcgg cgctttgaac aggaagcccg ggtcgtcggc aagcttctcg attgcaatgc
      721 gggtttatat gtggtcgatg tgcgcgaccc ggtactgggc aaacacaagg acgaattgac
      781 cgttctggcc agttgcggcc ggcccttgct gccgattctg aacttcatac atggagccga
      841 agggcgcacc gccgcatggc gcgacgcttt ggcgcggcta ggtttgcacg ttgtggtcga
      901 attcgacacc atcgcccccg aactggacgg cgaaacgcaa ctttacgata agctggcctt
      961 gctgctggac gcctatgctc cgcttttgca tttgttgaaa gccgatatcg cgcgacaacg
     1021 cgacattcgc catgccgacg ccgcccgcct tatcgcggaa ttgctggccg atgcggccgc
     1081 atggcgcctg acgagcgaac ccggcgcaca ggcggtgacg ctggccagcg aaacattgcg
     1141 cgaaaaaata agagcaaggg aagaccaatg cgtgcgggcg ctgctgaagc gctacaactt
     1201 tcatattcat gattttcccg cgcattcttt accattgaag ggggaacgct ggggcatgga
     1261 tttgtttcac ccccaggcgc tcaaggacgt cggcattcat gtaacaaagg gattggcggc
     1321 cggcgccatg gccggcgcgg ccatcgacgc tttcacagcc ggtttaagct tgggatcagc
     1381 cacgctgatt ggcgcgacgg cgggaggatt atggcaaggc atcgaccgcc tggggaaacg
     1441 cgttttaggg cgcctgaagg gataccgcga actcagcgtg gacaacaacg tgctggaact
     1501 gcttgcgttg cgtcagatcc atttgctgca ggctctggaa cgacgcggcc acgcggccca
     1561 gcagccaatc gtgctgacgt ccggcaccgc ccttgaaggc ttgcgcaacg cacggataat
     1621 gatcgaactc gacgaaatac gcagccaacc gtcatggtcc acgctgaccg atgaatacac
     1681 caacagcgcc cggcgcgaat ccgtgctgcg ctcgttgaca cacaaactgg aaaagtggtc
     1741 gcttacaaca tgacaggccg gtcaggcaaa tcattcgagt tgagttcgcc gtcagccaga
     1801 ggcgggcaat gtgtgcgcaa cgcctgctcg atggcatcga ccgccgccgt gacgccggcg
     1861 gcataatcgc cgcgttgcag acgcgctcgc aattgatcgc aaatctgttc ccacagggca
     1921 tttggagctc cgacgccacg gtcggcaatc aattcaatac gctgtctgtc caatgccaaa
     1981 tacagcaaaa cgccggtgtt atggtgcgta tcccaaacac gcaaacggcc gaatacctcg
     2041 agcgcccgta aatgactgtc gcgctcatga atcggcgata ccgcctcgac ggcgaccatt
     2101 aattcgcccg catgatcgag ttcgcctttg cgtatctgct ccgcgatgcc cgccaatacg
     2161 cgcggcgtga agtgcttgcg gcgcaaccag tgcccctgga cagtggccca tccagtaatt
     2221 tccttcaatc tgccaccgct ctgacgcacg cttgtcccca aaaatgaccc taccaactgc
     2281 cggaagcccc gccgccgcca ctac
//
LOCUS       DATCXU010000504         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_688190,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000504 DATCXU010000000
VERSION     DATCXU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688190"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VIR04_11340"
     CDS             <1..335
                     /locus_tag="VIR04_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461382.1"
                     /translation="RTVSAQLDQALKTYKEPGECVRAQGLGTGQRASFLVFDRINFTY
                     DPKCTLILQQTSPQSAAAGTAPYSDYAQVLWQGKRGADRREIFRLLRVGNTNSTKTSA
                     SPLYPAIH"
     gene            complement(295..1011)
                     /locus_tag="VIR04_11345"
     CDS             complement(295..1011)
                     /locus_tag="VIR04_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY9461383.1"
                     /translation="MKSIFAIRHVHFEDLGSFEAVAETHGFSVRYLEAGVDDLESSCR
                     QGCDLLVALGGPIGAYQEHLYPFLSSELRILESRLSRGLPTLGICLGAQLMARVLGAR
                     VYPGPVKELGWKPLALTREGRESSLSHLAAENTPVLHWHGDTFDLPDGATLLASTKDY
                     SNQAFAWSDAGLGLQFHPEAMASKIERWFIGHACELSAPGIPDLGQLRADTARYHAAL
                     ERSGSAFFSEWLGKAGLLTS"
     gene            complement(1008..2288)
                     /locus_tag="VIR04_11350"
     CDS             complement(1008..2288)
                     /locus_tag="VIR04_11350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY9461384.1"
                     /translation="MSSISSIVENKLASLPLPVQLVMPGGEKLGPPNPAIRLILKERK
                     TLFHLAAGQVGGVGSDYVEGRLDIEGSMRDLMRLAAALVPVSPIEAGRERWYKSITRR
                     SASMWRHTIERDARQIQFHYDLSDDFYALWLDPLRVYSCAYFRTPDMTVAQAQEAKLD
                     HICRKLGLTSGQRFLDVGAGWGGLLLWAAENYGVDATGITLSKHQHAYVNDLIQAKGL
                     SGRVRMNLIDYRQLDESQPYDKIASVGMFEHVGVAQLQSYFSKLRRLLRPGGLVLNHG
                     ITAGGIDNSELGSGMGDFIEKYIFPGGELTHISHVLENIALGGLEALDIENIRPHYAR
                     TLWAWSDSLEARLPQAREVLNTEQGERSLRAYRLYLAGCAMGFENGWVALHQVLAQPA
                     TSGRNDELDYPADLSYPWRRDYIYRNGTTGGSGL"
     gene            2503..2739
                     /gene="rpmB"
                     /locus_tag="VIR04_11355"
     CDS             2503..2739
                     /gene="rpmB"
                     /locus_tag="VIR04_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY9461385.1"
                     /translation="MARVCQVTGKGPIVGNNVSHANNKTRRRFLPNLQSRRFWVESEG
                     RWVRLRVCAKAIRTIDKNGIDSVLADMRARGQRA"
     gene            2774..2941
                     /gene="rpmG"
                     /locus_tag="VIR04_11360"
     CDS             2774..2941
                     /gene="rpmG"
                     /locus_tag="VIR04_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY9461386.1"
                     /translation="MAKGAREKIKLESTAGTGHFYTTTKNKRTMPEKMLIKKFDPVAR
                     KHVDYKETKLK"
     gene            3090..>4603
                     /gene="leuS"
                     /locus_tag="VIR04_11365"
     CDS             3090..>4603
                     /gene="leuS"
                     /locus_tag="VIR04_11365"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY9461387.1"
                     /translation="MQERYNPNVVEADAHKSWQAGDVYRVTEHATQADGTPKPKFYAC
                     SMLPYPSGKLHMGHVRNYTINDMMTRQLRMRGFNVLMPMGWDAFGMPAENAAIKSKVP
                     PAKWTYDNIAYMKRQMLAMGLAIDWSREMCACDPKYYKWNQWLFLKMLDKGIAYRKTQ
                     VVNWDPVDQTVLANEQVIDGRGWRSGALVEKREIPGYYLRITDYAAELLEQVQHGLPG
                     WPERVRLMQENWIGKSEGVRFAFTHSIKDSAGELIQDGRMYVFTTRADTIMGVTFCAV
                     APEHPLATLAAQTRPELAAFIQACKQGGTTEAELATREKEGMPTGLTVIHPITGASID
                     VWVGNYVLMSYGDGAVMGVPAHDERDFAFAKKYKLPIQQVVAIEGETYSTDQWQEWYS
                     DKSRGHAVNSGRYDGMTQKQAVDAVAGDLEKKGLGQKQTTWRLRDWGISRQRYWGTPI
                     PIIHCPACGAVPVPEKDLPVILPDNLIPDGSGNPLTKCEAFLSCQCPKCGGPARR"
BASE COUNT         1115 a         1373 c         1219 g          896 t
ORIGIN      
        1 accgaactgt ttcggcacag ctcgatcaag ccctgaaaac ctacaaagag ccgggcgaat
       61 gcgtgcgcgc tcaaggcctg ggcacgggtc aacgcgcctc gtttctggta tttgatcgta
      121 tcaacttcac gtacgatcca aaatgcacgc tgatcctgca gcagacatcg ccgcaaagcg
      181 ccgcggccgg caccgcgcct tattccgatt acgcccaagt gctctggcaa ggaaaacgcg
      241 gggccgaccg acgcgaaata ttccgcctgc tccgagttgg gaatacgaat tccactaaga
      301 cgtcagcaag cccgctttac ccagccattc actaaaaaac gcgcttccgg atcgttccag
      361 cgccgcatga tagcgggcgg tgtccgcgcg caactgccca agatcgggta tgccgggcgc
      421 ggacagctcg caggcatggc caataaacca ccgttcaatc ttcgatgcca tcgcttcggg
      481 atggaactgc aacccaagcc ccgcatcgga ccatgcgaac gcctgattgg aatagtcctt
      541 tgtcgacgca agcagcgtcg ctccgtcggg taaatcgaac gtatcgccat gccaatgcaa
      601 taccggcgta ttttccgccg ccaaatggga cagcgaagat tcacgccctt cccgcgtcag
      661 cgccagcggc ttccatccca gttctttcac agggcccgga tagacgcgcg cgccaagcac
      721 acgcgccatc aattgggcgc caaggcatat tcccagcgtc ggaagcccgc gggaaagccg
      781 gctttccaga attcgcaatt cgcttgaaag aaacgggtat aaatgctcct ggtaggcgcc
      841 gatcggtccg ccaagcgcca caagaagatc gcaaccctgc cggcacgacg attccaggtc
      901 atcaacgccc gcttcaaggt agcgcacgga aaacccgtgc gtttctgcga cggcttcaaa
      961 actgcccaga tcttcgaagt gcacatgccg aatggcgaaa atcgatttca taaaccagat
     1021 ccacccgtcg tgccgttgcg gtaaatataa tcgcgccgcc acggataaga taaatcagcg
     1081 ggataatcaa gctcatcatt gcgcccggac gtcgcgggtt gcgccagcac ctgatgcagc
     1141 gccacccagc cattttcaaa ccccatggcg catcccgcca gatacaggcg ataagcgcgc
     1201 aatgatcgtt ccccctgctc ggtgttcaga acttccctgg cctgcggcag gcgagcctcc
     1261 agagagtcgc tccaggccca cagcgtacgc gcgtaatgag gccgaatgtt ctcgatatcg
     1321 agcgcttcca gccctcccag agcgatattt tccagcacat ggcttatgtg cgtcagttcg
     1381 cctcctggga aaatgtattt ctcgatgaaa tcccccatcc ctgaacccag ctcgctgttg
     1441 tcgatccctc cggcggtgat gccgtgattg agcaccagcc cgccgggccg aagcaagcgc
     1501 cgcaatttcg aaaaataact ctgaagctgc gccacgccca catgctcgaa cataccgacc
     1561 gatgctatct tgtcataggg ttgcgattcg tccagttgcc gataatcaat caagttcatg
     1621 cgcacgcgtc cgcttaaacc tttggcctgg atcagatcgt ttacataagc atgctgatgt
     1681 tttgaaaggg taatgccggt cgcatcgacg ccgtagtttt cagccgccca taacagcagc
     1741 cctccccaac cggcgcctac atcaagaaac cgctgacctg aagtcaggcc cagcttgcgg
     1801 caaatgtgat ccagcttggc ttcctgcgct tgcgcgactg tcatatcggg cgtgcggaaa
     1861 taggcacagg aatatacgcg aagaggatct agccacaagg cataaaagtc atcggacagg
     1921 tcgtagtgga attgtatttg gcgcgcatcg cgttctatgg tgtggcgcca catggaagcg
     1981 ctgcgccgcg taatactttt gtaccatcgc tcccggccgg cctcgatcgg cgagaccggc
     2041 acgagagccg cagcaaggcg catcaggtcg cgcatcgatc cctcgatgtc gagcctgccc
     2101 tcgacatagt ccgagcccac tccgccaact tgtcccgccg cgagatgaaa aagagttttg
     2161 cgttctttga gaatcaggcg aatcgccgga ttcggcggcc ccaatttttc gccgccaggc
     2221 atgacaagct ggacgggcag aggcaacgaa gcgagcttat tttcaacaat ggacgagatc
     2281 gaactcacaa ccggctccct tataaataaa acacgcaacg gcaaaaataa tgccacaatt
     2341 catcgataaa ttgtatcaac cggactgcag cgcgatgaaa ttttatgccg ggccacgttc
     2401 gacctattaa ccgcaggggc cgccgtttga ttaaacagct aaatatggta tagaatggca
     2461 ggctttacaa atatacatac ccaaaaccca agaggtgcaa ccatggcacg cgtatgccaa
     2521 gttaccggca aaggcccaat agtgggcaac aatgtttcgc acgcgaacaa taaaacccgt
     2581 cgccgttttc tgccaaattt gcagtcccgt cgtttttggg tcgaaagcga gggccgctgg
     2641 gttcgcctgc gtgtgtgcgc gaaagcgatc cgcacgatcg acaaaaacgg catcgattca
     2701 gtgctggccg acatgcgtgc ccgtggtcag cgggcataga ccgcgctagt gttctaatcc
     2761 tcaggagctt attatggcaa aaggcgcacg cgagaagatc aaactcgaat ccaccgctgg
     2821 aaccggtcat ttctacacga ccactaaaaa caaacgcacc atgcccgaga aaatgctgat
     2881 caagaaattt gatccggttg ctcgcaaaca tgtggactat aaagaaacca agctgaaata
     2941 gtccttgctt atgcgaaaac cctgccgcgg cagggttttt tcgtttgcgc gacacccacc
     3001 ggccatgact ggcttccagc gccacgtggg gcagcaccct ataatgcaac gttcggttct
     3061 ttcccctctt ttttcaagta cgcaatctta tgcaggaacg ctataacccc aacgtcgtcg
     3121 aagccgacgc ccataaaagc tggcaagcgg gcgacgtcta tcgcgtcacc gagcacgcca
     3181 cgcaagccga cggcacgccc aaacccaagt tttacgcctg ttccatgctg ccctacccca
     3241 gcggcaaact gcatatgggt cacgtgcgca actacaccat caacgacatg atgacgcgtc
     3301 agttgcgcat gcgcgggttt aacgtcttga tgcctatggg ttgggacgcg tttggcatgc
     3361 ctgccgaaaa tgccgctatc aaatccaaag tgccgcccgc aaagtggacg tatgacaaca
     3421 tcgcctacat gaagaggcaa atgttggcga tgggcctggc gatcgactgg tcgcgcgaaa
     3481 tgtgcgcgtg cgatcccaaa tactacaaat ggaaccagtg gctttttttg aagatgctgg
     3541 ataaaggcat tgcatatcga aagacccaag tcgttaactg ggatccggtc gaccagacag
     3601 tgttggccaa cgagcaggtc attgacgggc gcggctggcg ctccggcgct ctggtcgaaa
     3661 aacgtgaaat tccgggctat tacctgcgca tcaccgatta cgcggcggag ttgcttgaac
     3721 aggttcagca tggtttgcca ggctggcccg agcgcgtgcg tttaatgcag gaaaactgga
     3781 tcggcaaaag cgaaggagtc cgctttgctt tcactcattc tataaaagac agcgctggcg
     3841 agttgattca agacggtcgc atgtatgtct tcaccacacg cgccgatacg attatgggcg
     3901 tgacattttg cgccgtcgcg cccgaacacc cgctggccac cctggcggcg caaacccgtc
     3961 cggaactggc cgctttcata caggcctgca aacaaggcgg aacgaccgag gccgaactgg
     4021 ccacacgcga aaaagaaggg atgcccacag gactgacggt catccacccc attaccggag
     4081 cctctatcga cgtatgggtc ggcaactacg tgctcatgag ctatggagat ggagcggtga
     4141 tgggcgtgcc cgctcacgac gaacgcgatt tcgccttcgc caaaaaatac aagctgccga
     4201 ttcagcaagt cgtcgccatt gaaggcgaaa cctattcgac cgaccaatgg caggaatggt
     4261 atagcgacaa atctcgcgga cacgccgtca actcgggtcg ctacgacggc atgacgcaaa
     4321 aacaggcggt cgacgcggtt gccggggatc tcgagaaaaa ggggctgggc cagaaacaaa
     4381 caacgtggcg tctgcgcgac tggggcattt cacgccagcg ctattggggt actccgattc
     4441 cgatcatcca ctgcccggcc tgcggagcgg tgcccgttcc cgaaaaagat ttgccggtta
     4501 ttttgcccga caacctgatt cccgatggca gcggaaaccc gctgaccaaa tgcgaagcct
     4561 tcttgtcatg ccagtgcccc aaatgcggcg ggccggcccg ccg
//
LOCUS       DATCXU010000505         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_532133,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000505 DATCXU010000000
VERSION     DATCXU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532133"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1392
                     /gene="radA"
                     /locus_tag="VIR04_11370"
     CDS             40..1392
                     /gene="radA"
                     /locus_tag="VIR04_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY9461388.1"
                     /translation="MAKSRTIYVCNECGGSCPKWEGRCPHCGSWNTLQESPELSVAAH
                     RFAPLAEASPVRSLSEIDAHELPRQPSGIAEFDRVLGGGLVAGAVVLIGGDPGIGKST
                     LLLQALASLSSVVKVLYVTGEESAEQVALRARRLELSCGDVQVLAEIRLEAIIAALTR
                     QNPGVAVIDSIQTLYSSELSAAPGSVSQVRECAARLTRLAKQTGITIVLIGHVTKDGS
                     LAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTDRGLRGVANPS
                     ALFLSRHDQEVAGSCVMATQEGTRPLLVEIQALVDSAHVPNSRRLSVGLESTRLAMLL
                     AVLHRHAGVVTFDQDVFVNAVGGVKISEPAADLAVLLAIISSLRNRPLPKGLVVFGEV
                     GLAGEIRPAPRGQERLKEAAKLGFGLAMIPAANAPRHPIEGLEIWAVDRIDAAIERLH
                     "
     gene            1450..>2621
                     /locus_tag="VIR04_11375"
     CDS             1450..>2621
                     /locus_tag="VIR04_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY9461389.1"
                     /translation="MFFAWFSDLLAGTRALRRDWRSGELRLLTLALIVAVAAVTSVGF
                     LTDRVGRALERNSAQMLGGDLALQADEPIPAEFVRYALALGLDATQTQQFPSMVSSGT
                     QTQLASLKAVSPGYPLRGALRVSETAADPGASTHLVPSSGTVWVDPRVLGLLGLKLGD
                     SLALGDIRVVIAKVLTYEPDRGMQFVNVAPRVMMNLDDLLRTGLVAPGSRIRYELLLA
                     GRDRAVQTYRTWLTPRLKRGQELRTLDSTRPEVQQALERAHQFLMLVALLAVMIASVA
                     VALAARRFNLRHQDGVAIMRCLGATKSQLNRLLWMEFLSLAVVSALMGTVIGYGMQQG
                     VASVLAPWIHTVLPAPSWGPARQGLATGLLLLFGFILPPLLALRDVAPVHVLRRGAP"
BASE COUNT          434 a          703 c          866 g          618 t
ORIGIN      
        1 gatggcggcg gtagtcttgc gggtcgctaa acgttgacaa tggccaaatc cagaaccatt
       61 tacgtatgta acgaatgtgg cgggagctgc ccgaagtggg aagggcggtg tccacattgc
      121 ggttcgtgga acaccttgca ggaatcgccc gaactaagcg ttgccgcgca ccgttttgct
      181 cccttggccg aggccagccc cgttcgcagt ttgtctgaaa ttgatgcgca tgaattgcct
      241 cgccagcctt ccggaattgc ggaattcgat cgtgttctgg gcggtggtct ggtggcgggc
      301 gccgtggtgc tgatcggtgg tgatccggga atcggcaagt cgactttgct attgcaggct
      361 ttggcgtcgt tgtcctcggt cgtaaaggtt ttgtacgtta ccggcgaaga gtccgccgaa
      421 caggtggcgt tgcgggccag acgtctcgag ttgtcttgcg gcgatgtcca ggtgctggcc
      481 gaaatccgcc ttgaagcaat tattgccgcg ctgacacggc aaaaccccgg cgtggcggtt
      541 attgattcca tacaaacttt gtattcaagc gagcttagcg ccgcgccggg gtctgtgtcc
      601 caggtcagag aatgcgccgc gcggcttacg cgattggcca agcagaccgg tatcaccatt
      661 gtcctgatcg gccacgtcac caaagatgga tcgctggcgg ggccgcgcgt gctggaacac
      721 atcgttgata cggtattgta ttttgaaggc gacactcatt cctcgtttcg cctggtgcgg
      781 gcattcaaga atcgattcgg cgcagtcaac gagctgggcg tgttcgccat gacggatcgc
      841 ggcctgcggg gcgtggctaa cccttcggcc ttgtttctgt cgcgtcacga tcaagaggtg
      901 gccggctcgt gcgtgatggc cactcaggaa ggaacccgcc cattgctggt cgaaattcag
      961 gcgctggtcg acagtgcgca cgttcccaat tcccggcgtt tgtcggtagg ccttgaaagc
     1021 acacggctgg ccatgttgct ggccgtcttg catcggcacg cgggcgtggt cacgtttgat
     1081 caggatgtct ttgtgaatgc ggtgggcggt gtgaaaatta gcgagcccgc cgcggatctg
     1141 gctgtgctgc ttgccatcat atcgtcgttg cgcaacaggc ctttgcccaa agggctcgtt
     1201 gtttttgggg aagtgggtct ggccggcgag attcggccgg cgccgcgcgg tcaggagcgc
     1261 cttaaagagg ccgccaaact gggctttggc ctggcaatga ttccggcggc gaacgcgccc
     1321 cgtcatccga tcgagggtct ggaaatttgg gcggtggacc gcatcgatgc agccatcgaa
     1381 cgattgcatt gaacgtcgtt gatgtgtaaa aagaaatcgt ggccagaggt ttttcgaggc
     1441 gtgatcgtca tgtttttcgc gtggttttcc gatttgttgg cgggcacgcg cgctttgcgg
     1501 cgcgactggc gttctggcga attgcgcttg ctgacgctgg ctttgattgt cgcggtagcg
     1561 gcggtaacca gcgtgggttt tctgaccgat cgcgtggggc gcgcgctgga gcggaattcg
     1621 gcgcagatgc tgggcggcga tctggccttg caagccgatg aacccattcc ggccgagttc
     1681 gtgcgttatg cgctggcgct ggggcttgac gccacccaga cgcagcagtt cccttcaatg
     1741 gtcagcagcg gcacgcagac gcaattggcc tcgttaaagg cggtctcgcc gggctatccc
     1801 ttgcgcgggg ccttgcgggt gtctgaaaca gccgctgacc cgggcgcctc cacgcatttg
     1861 gttccctctt ctggcacggt ctgggtcgac ccgcgggtgc tgggcttgtt gggccttaaa
     1921 ttgggcgatt cgcttgcact gggcgatatc agggtggtga ttgccaaggt actgacctac
     1981 gagcctgacc gtggcatgca gtttgtgaat gtggcgccgc gcgtcatgat gaaccttgac
     2041 gacttgcttc gcaccggtct ggttgctccg ggcagcagga tacgctatga gctgctgttg
     2101 gcgggtcgcg accgcgccgt ccagacgtac agaacatggc tcactccacg attgaaacgg
     2161 ggccaggaat taagaacgct ggattccaca cgccctgagg tacagcaggc gctcgaacga
     2221 gcgcaccagt ttttgatgct ggtggcgtta ctcgcggtca tgattgcctc cgttgccgtt
     2281 gctctggcgg cccgccggtt caatttgcgc catcaggacg gcgtcgcaat catgcgctgt
     2341 ctgggtgcaa caaaatcgca attgaaccgc ctgttgtgga tggaatttct atctttggcc
     2401 gttgtttcgg cattgatggg aacagtgatc ggatacggca tgcaacaggg cgtggcgtcc
     2461 gtgctggcgc cctggattca cacagttttg cctgcgccat cgtggggtcc cgcgcggcaa
     2521 ggtctggcaa caggtctgtt attgttattc ggctttattt tgccgccatt attggcgttg
     2581 cgcgacgtgg cgccggtgca cgtgttgcgg cgtggcgcgc c
//
LOCUS       DATCXU010000506         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1129139,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000506 DATCXU010000000
VERSION     DATCXU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129139"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VIR04_11380"
     CDS             complement(<1..336)
                     /locus_tag="VIR04_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9461390.1"
                     /translation="MTVLKNVSNPLLTLSDVNTWYGGAHILHGIALQVGRGEVVALMG
                     RNGAGKSTTLKSIMGLARKRLGQIQFMGHSVIRLAPYQIARLGLGYVPEDRRIFTDLT
                     VEENLAVARQ"
     gene            complement(333..1109)
                     /locus_tag="VIR04_11385"
     CDS             complement(333..1109)
                     /locus_tag="VIR04_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY9461391.1"
                     /translation="MSLLSVKNLKKQFGGNVAVDGISFDLAAGELLALIGPNGAGKST
                     TFNMIGGQLRASSGSVLLNGSELLGTSSREIARLGVGRTFQIAATFSSLTVLENIQVA
                     LLARQRKIYSFWRPAAGMVRDRALALLDQVGMLAQALRPCSELAYGDVKRVELAIALA
                     AEPQLLLMDEPTAGMAASERHALIELVRQLVHQHNLAVLFTEHSMDVVFAYADRIIVL
                     ARGQLIAQGSADQVRNNAKAQEVYFGTGKTFEPGAECATS"
     gene            complement(1106..>2297)
                     /locus_tag="VIR04_11390"
     CDS             complement(1106..>2297)
                     /locus_tag="VIR04_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461392.1"
                     /translation="GSIPGAYLAALIIAELKAFCIGLGVVSFGDVTLAFPKLTLVIEF
                     VFMALVLVIRPWGLLGKPLAFIRSAGSQDAPLRRATPWFNGFMLAILLGLAALPLLSE
                     AVPYASVLALDFIVAALFAASLHFMMGPGGMPSFGHAAYFGLGAYGAALLLASAGWPM
                     IPAVMAAPVFAGLGAVIFGWFCVRLSGIYLAMLTLAFAQIIWSIVYQWDAVTGGSNGI
                     IGVWPAAWLSGSRYYLFALAMVALSVFVLRRLVFSAFGYALRASRDSALRADAIGIDV
                     KRVQWLAFVVAGLFAGLAGALFVFSKGGVSPEVITVGKSVDGLVMVLLGGVQTLTGPL
                     VGAAAFTMLQSYFIGITQYWHALLGGVILLLVLAFPQGIAGFIQQIADGLKAMRHPGV
                     AEDA"
BASE COUNT          584 a          741 c          596 g          376 t
ORIGIN      
        1 ctggcgggcc acagccaggt tttcctcgac ggtaagatcg gtgaaaatcc gcctgtcctc
       61 gggcacatag cccaatccca ggcgggcaat ttgatacggc gcaagcctaa tgaccgaatg
      121 gcccatgaac tgaatctgac ccaaacgttt tcgcgccagg cccatgatgg atttcaaggt
      181 ggttgacttg ccggcgccat tacgacccat tagcgccaca acttcgcccc ggccgacttg
      241 tagcgcaatc ccatgcaaga tgtgcgcccc gccgtaccag gtattgacgt cggacagcgt
      301 cagtaaaggg ttggagacat ttttcaaaac agtcatgagg ttgcgcactc cgctccgggc
      361 tcaaacgttt tgccggtgcc aaaataaacc tcctgggcct ttgcattgtt tcgcacctgg
      421 tcagcgcttc cctgggcgat gagctggccg cgagccagaa caatgatgcg atcggcatag
      481 gcaaacacga catccatgct atgttccgtg aacagaaccg ccagattatg ttggtgcacc
      541 agttgccgaa ccagttcaat cagcgcatgg cgctcgctgg ccgccatgcc tgccgtgggt
      601 tcgtccataa gcagcaactg cggttcggcc gccagcgcaa tagccagttc cacgcgcttt
      661 acatcgccat aagccagttc gctacagggg cgaagagcct gcgcgagcat tccgacctgg
      721 tcaagcaagg caagcgctct gtcgcgcacc atgccggcag cagggcgcca aaacgaatag
      781 atctttcgct gtcgcgccaa tagggctacc tgaatatttt caagcaccgt aagcgacgag
      841 aacgtggcgg cgatctgaaa cgtgcgtcca acccccaacc gcgcaatctc gcgcgatgac
      901 gttcccagca actccgaacc gtttagcaga acggaccctg aactcgcccg caactgacct
      961 ccgatcatat tgaatgtcgt cgacttgccg gctccgttag gcccgatcag ggccaacagc
     1021 tcgccagcgg caagatcaaa gctgatgccg tccaccgcca cgttgccgcc gaattgcttt
     1081 ttcaggtttt taacggacag caggctcatg cgtcctccgc cacgccggga tggcgcatcg
     1141 ccttcaggcc atcggcaatt tgctggataa aaccggcaat cccttgcggg aaagccagca
     1201 cgagcaacag gatcacgcca cccagcaggg catgccaata ctgggtaata ccaataaaat
     1261 agctttgcag catcgtaaaa gcggcagcgc caaccagggg cccggtcaac gtttgcacgc
     1321 cacccagcaa aaccatgacc aggccatcca ccgacttgcc tactgtgatg acttcaggag
     1381 acacgccgcc tttggaaaat acgaatagcg cccccgccag tcccgcaaac aggcccgcca
     1441 ccacaaacgc aagccactga actcttttga catcaatgcc gatcgcatca gcgcgcagcg
     1501 ccgaatcgcg gctagcgcgc agcgcatagc caaatgccga aaaaaccagt cggcgcaata
     1561 cgaaaaccga cagtgctacc atcgccagcg cgaaaaggta atagcggctg ccagagagcc
     1621 aggcggccgg ccacacgccg atgatgccgt tcgatccgcc cgtcaccgcg tcccattggt
     1681 atacgattga ccagataatc tgggcaaacg ccagcgtaag catggctaaa taaatgcccg
     1741 acaaacgcac gcaaaaccag ccaaaaatta ccgcgcccag gcccgcaaaa acaggcgccg
     1801 ccatgactgc gggaatcatt ggccagcccg ccgacgctaa tagcaaggcg gcgccgtatg
     1861 cgccaagacc gaaataagcc gcatgtccga acgacggcat gccccccggc cccatcatga
     1921 aatgcaagct cgccgcgaaa agcgccgcga caataaagtc gagcgccagc accgaggcat
     1981 acgggaccgc ttccgacaac aaaggcagcg ccgcaagccc caaaaggatc gccagcataa
     2041 atccgttgaa ccatggcgtc gcccggcgca agggcgcatc ttgcgagccc gcacttcgga
     2101 tgaacgccag aggtttgccc agcaagcccc acggtctgat cacaagaacc agcgccatga
     2161 aaacgaattc aatcacgagc gtcagcttgg gaaaggcaag ggttacatcg ccaaacgaaa
     2221 ccaccccgag gccaatacaa aacgctttaa gctcggcaat gatgagtgcg gccagataag
     2281 cccccggtat tgacccc
//
LOCUS       DATCXU010000507         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_377286,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000507 DATCXU010000000
VERSION     DATCXU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377286"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /gene="guaA"
                     /locus_tag="VIR04_11395"
     CDS             complement(<1..520)
                     /gene="guaA"
                     /locus_tag="VIR04_11395"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY9461393.1"
                     /translation="MHQRILILDYGSQVTQLIARRVRDSGVYCEIHPGDVDDAFIAAQ
                     EGLKGIILSGSHASAYGDESPKVPAGVFKAGVPVLGICYGMQSMASQLGGKVEWSDRR
                     EFGYAEVRARGHTRLLEGISDFSSADGHGMLKVWMSHGDKVTELPPGFKLMASTPSCP
                     IAGMADEERGFYA"
     gene            637..1377
                     /locus_tag="VIR04_11400"
     CDS             637..1377
                     /locus_tag="VIR04_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY9461394.1"
                     /translation="MPAQQNAQTQTKTFVLVHGAWHGGWCWARVAERLRALGHRVFTP
                     TQTGLGERKHLLGATITLDTFVQDIANVLLWEDLNNVILVGHSFAGLSITGVADILPQ
                     RILHLVYLDAFILDSGISTFDTLPKETVEKLREAAEERGQGLALPAPKPKAFGLFEPA
                     DIAFIAARLTPQPLRTYETALHLNNAIGNGLPCTYLHSIAPLFAPVEATWQWVEKQPY
                     WTRIEINAGHDSMVSHPDLVARHLLNVA"
     gene            complement(1427..>2751)
                     /gene="guaB"
                     /locus_tag="VIR04_11405"
     CDS             complement(1427..>2751)
                     /gene="guaB"
                     /locus_tag="VIR04_11405"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY9461395.1"
                     /translation="AMDTVTESRLAIAMAQEGGIGIIHKNLSADDQAREAARVKRHEF
                     GIVIDPVTVTPAMKVRDAIELQRQHGISGLPVVEAGKLVGIVTNRDLRFEDRLDVPIR
                     DVMTPQDRLVTMKEGATLDEAQALMHKHRLERVLIVNDKFQLRGLATVKDIVKNTEHP
                     NASKDSQGQLRVGAAVGVGEGTEERVEKLVNAGVDVIVVDTAHGHSAGVIERVRWVKK
                     NYPKIQVIGGNIATASAAKALVAVGADCVKVGIGPGSICTTRIVAGVGVPQITAIADV
                     AKALEGTGVPLIADGGIRYSGDISKALAAGASTCMMGGMFAGTEEAPGEVVLFQGRSY
                     KSYRGMGSVGAMTAGSADRYFQDPENNAEKMVPEGIEGRVPYKGSVIAIIYQLVGGIR
                     ASMGYCGCASIPEMHDKAEFVEITAAGVRESHVHDVQIVKEAPNYRAE"
BASE COUNT          612 a          870 c          711 g          558 t
ORIGIN      
        1 ccgcgtaaaa gccgcgctct tcatcggcca tgcccgcaat ggggcaggat ggtgtcgagg
       61 ccataagctt gaatccgggc ggcaattcag tgaccttgtc gccatggctc atccacacct
      121 taagcattcc atgaccatca gcgctggaaa aatccgatat tccttcaagc agtcgcgtat
      181 ggccgcgcgc gcgcacttcg gcataaccga attcgcgccg atcggaccac tcgaccttac
      241 cgcccaattg cgacgccatg gactgcatgc cataacaaat tcccaaaacg ggcacgccgg
      301 ctttaaatac cccggccggc accttgggcg actcgtcgcc ataggccgac gcatggctcc
      361 cggataaaat gattcctttc agaccttcct gggccgcaat aaaggcatcg tcaacgtcgc
      421 ccgggtggat ctcacaatac accccgctat cgcgtacacg acgtgctata agttgggtaa
      481 cctgcgagcc ataatcaagg ataagaattc gctgatgcat agtgtctaaa acgtcgaatg
      541 aaaaagagcc aaggcgttaa tgtaacgtat tgagctaaga tataagcgta aacataggcg
      601 ccagtaacgc cgagccatcg tttttacgac aaaacaatgc ctgcacagca aaacgcgcaa
      661 acccaaacaa aaacgttcgt gcttgtacac ggagcgtggc acggaggatg gtgctgggcg
      721 cgggttgccg aacggctgcg agcgctgggg caccgggtat tcacgccgac tcaaaccggt
      781 ttgggcgaac gcaagcacct gcttggcgcc accatcacgc tggacacctt cgtccaggac
      841 attgcaaatg tgctgctctg ggaagacctg aacaatgtca ttctggtcgg acacagtttt
      901 gcgggcctgt ccattaccgg cgtggccgat attttgccgc aacgcattct tcacctggtt
      961 tatctggacg ctttcattct ggacagcgga atttccacct tcgacacgct gccaaaagaa
     1021 accgtggaaa aactgcgcga ggcggcggag gaacgcggcc agggcctggc cttgcctgcg
     1081 cccaagccca aagcattcgg cctgttcgaa cccgcggata ttgcttttat agcggcgcgc
     1141 ctgacgcccc agccactgcg cacctacgag acggcacttc atttgaacaa tgccatcggc
     1201 aacggattgc catgcaccta cctgcacagc atcgcgcctt tgtttgcgcc cgtggaagcc
     1261 acctggcaat gggtcgaaaa acagccgtac tggacacgca tcgaaattaa tgcgggtcat
     1321 gactccatgg tcagccatcc ggacctggtc gccagacacc ttctaaacgt cgcctgacgc
     1381 cagcgatctg gcatttgccg gagtgtgacc acatttaccg gaagggctat tcggcccgat
     1441 agttgggcgc ttctttgaca atttgcacat cgtgcacatg cgactcgcgt acgccggctg
     1501 cggtgatttc aacaaactcg gccttgtcgt gcatctcggg aatggacgcg catccgcaat
     1561 agcccatcga ggcacgaatg ccgccgacca gttgatagat aatggcgatt acgctgccct
     1621 tgtagggaac acggccttca atgccctcgg ggaccatttt ttctgcgtta ttttcgggat
     1681 cttgaaaata acgatcagcg gacccggccg tcatggcgcc cacgctgccc atgccacggt
     1741 acgacttgta cgagcgaccc tgaaacagca ccacttcgcc aggcgcttct tcggtgcccg
     1801 caaacatgcc gcccatcatg cacgtagagg cgcctgcagc cagcgccttg gaaatatcgc
     1861 ccgaataacg gatgccgcca tcggcaatca atggaacgcc cgtgccttcc agcgctttcg
     1921 caacgtcggc aatcgccgta atctgcggca cgccaacgcc ggccacgata cgcgtcgtgc
     1981 aaatagagcc agggccgatc cccactttaa cgcagtcggc cccgaccgcc accagcgctt
     2041 tggccgcgct cgcggtagcg atattgccgc cgataacctg aatcttcggg tagtttttct
     2101 ttacccaccg aacgcgttca atcacgccgg ctgaatggcc atgcgccgta tcgacgacaa
     2161 tcacgtcaac gcccgcattc accagctttt ccacacgctc ttcagtgccc tcgcccacgc
     2221 caaccgcagc gccaacccgc aactgtccct ggctgtcttt cgacgcattg gggtgttccg
     2281 tattcttgac aatgtcttta accgtcgcca gaccgcgaag ctggaacttg tcgttgacga
     2341 tcaacacgcg ctccaggcgg tgcttatgca taagggcctg ggcctcgtcc agcgtcgcgc
     2401 cttccttcat ggtgaccagt cgatcctgcg gggtcatgac gtcgcgtata ggaacatcca
     2461 gccgatcttc aaagcgcaaa tcgcgattgg tcacaatacc cacaagcttg ccggcctcga
     2521 ccacaggcag gcccgaaatg ccatgctgac gctgcaactc aatagcgtcg cgcactttca
     2581 tcgctggagt gaccgtaacc gggtcaataa caatgccgaa ttcgtgccgc ttcacccgcg
     2641 ccgcttcgcg cgcctgatcg tcggcgctaa ggttcttgtg aataatgcca atgccgccct
     2701 cttgcgccat cgcaatcgca agccgcgatt ccgtgacggt atccatagcc g
//
LOCUS       DATCXU010000508         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_772888,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000508 DATCXU010000000
VERSION     DATCXU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772888"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1067)
                     /locus_tag="VIR04_11410"
     CDS             complement(201..1067)
                     /locus_tag="VIR04_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY9461396.1"
                     /translation="MDRQIRYFLKIAQTSSLSRAAEELDLTQSGLSRQLAALEAFFGK
                     PLFVRTGRGVKLTGAGEKLQQGTRRAFEQIDASVESIKEKEGVTEGGLRIATIHTLSY
                     YFVSALLAKFIGQRGRVNLSVMARSSPDVVDLVEKGKADVGFVYDSAVASDLLESVPL
                     FDDGMCLITAQGDFSGEATVDLTDVKLPLVGFPEAFALRKMLKSAGLDGQVVAEANTV
                     DAMLQLVASGIGSCILPERIPTQLLAEYQLTKVILHAPVLMRRVVAIVRSNATAPSMA
                     RQLLEMARVSVA"
     gene            complement(1170..1379)
                     /locus_tag="VIR04_11415"
     CDS             complement(1170..1379)
                     /locus_tag="VIR04_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY9461397.1"
                     /translation="MQTEFGIVKWFNNEKGFGFIKPESGGKDLFAHHTDIIGTGFKSL
                     EENQRVSYVAAEGQKGPQAKSIQVV"
     gene            1624..2175
                     /locus_tag="VIR04_11420"
     CDS             1624..2175
                     /locus_tag="VIR04_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY9461398.1"
                     /translation="MRKLTRNFYNRDTLQVAEELLGKLLVHRVDGIKRIGRIVEVEAY
                     LGPHDHAAHTSKGITPRTRAMYGPPGHAYIYLVYGMHHCMNVVTEPDGHGSGVLLRAL
                     EPVQNLQGSASGPGLLCKAMGIDRQYYGHDLCSSDLYLASPATKLDISIMRRPRIGVA
                     YAGEWADKPLRLYIEGNKFISRK"
BASE COUNT          572 a          643 c          573 g          518 t
ORIGIN      
        1 ccgccgtggc gcgctgatcg aaatagttct tttctcttaa tgttgcgtcc atatcgctct
       61 cctgtgcgaa ttgcctggca atccctcccc cggtttaagg gtcaggattg atctgcgtca
      121 attctatgag caggagccgc ggagaaccat gcagcagcag tcataccaga tatgccgcgc
      181 cgcaagcatt gcgatgaaaa tcaggccacc gagactcggg ccatctcgag gagttgtctc
      241 gccatcgaag gcgcggtggc gttgctccgg acgatggcga ccacgcgacg cattaatacc
      301 ggcgcatgca agattacttt ggtcagttga tactcggcca gcaattgagt gggaatgcgt
      361 tcgggcaata tacagcttcc gatacccgag gccacgagtt gcagcatggc gtccaccgta
      421 ttggcttccg cgactacctg cccatccaga cccgcgcttt tcaacatttt gcgcaaagcg
      481 aaagcttcag gaaagccaac cagcggcaat ttcacatcgg tcaaatcgac cgtcgcttcg
      541 ccgctgaaat cgccttgcgc ggtaatcaga cacatgccgt cgtcgaacaa cggcacggac
      601 tccagtaaat cggatgccac ggcggaatcg tacacaaagc cgacatcggc tttccccttt
      661 tccaccaaat cgaccacgtc gggggaactg cgagccatga ctgacaagtt aacgcggcca
      721 cgctgcccaa taaattttgc cagtagcgcc gatacgaagt aatagcttag cgtgtgaatc
      781 gttgcaatac gcaagccgcc ttcagtcact ccctcttttt ctttgattga ctcaacgctc
      841 gcatcgattt gttcgaaggc acgccgtgtt ccttgctgca gcttctcgcc tgcgcccgtc
      901 aatttaacgc cgcggccagt gcgaacaaat agcggcttgc caaaaaaggc ttctagcgct
      961 gccaactgac ggctaaggcc ggactgtgtc aaatccagct cctcggccgc acgcgacaac
     1021 gaagaagtct gcgcaatctt caagaaatac cgaatttgtc tgtccatgcc acccttaccc
     1081 gcaaccaagt attcagttaa aacaaaccgg gcagcaaaaa gcccgcctgt aaaggcgggc
     1141 tttaatcaaa ctatttaagc taattaagct taaacgacct gaatcgattt ggcttgaggg
     1201 cctttttggc cttcggccgc cacgtaggaa acgcgttggt tttcttcgag cgatttaaag
     1261 ccagtaccga tgatgtcggt gtgatgagcg aacaaatctt tgccgcccga ttcaggcttg
     1321 atgaaaccaa agcctttttc gttgttgaac catttaacaa taccaaattc tgtttgcatt
     1381 tttgcaatcc ctaaaaatta cgagcaatat gcccaactaa aagactatca aggagtagag
     1441 atgagagcaa aaaagtactg tttttggtac tggattgtac aaacttcaac gaacttgaaa
     1501 atctaggggg ctagtgtatt agttttgaca actgatgtca acaaccattt taaacgtact
     1561 atggcaggtc ggcaacgtac cttttatgcc gtgcagaaac gtttataacc cacctgcgat
     1621 gcaatgcgaa aactaactcg taacttttat aaccgcgaca ctctccaggt cgctgaagaa
     1681 cttctgggga aactgctggt tcatcgtgta gacggcatca agcgcattgg ccggatcgtc
     1741 gaagtagaag cctatctggg cccgcacgat catgcggccc acacatccaa aggcattaca
     1801 cctcgtacgc gggccatgta cggccctccc ggccacgcct atatatacct ggtgtacgga
     1861 atgcatcact gcatgaatgt cgtcaccgag cccgacggtc acggctccgg ggtgctgctg
     1921 cgagccctgg agccggttca aaatcttcag ggaagcgcca gcggacccgg actgttgtgc
     1981 aaggctatgg gcattgatcg ccaatattac gggcacgatc tgtgcagcag cgatttatat
     2041 ctggctagcc ccgccaccaa gctggacatc tcgattatgc gccggccgcg gatcggggtt
     2101 gcctacgctg gcgaatgggc cgacaagccc ttgcgccttt atatcgaggg caataaattt
     2161 atatcaagaa aataacagac agcgcccagc cctaaggagc aagccgaaag cctgtgtcca
     2221 tgccccaacg gtcagtgcgg cgaagccaca cggtgtagca ggtgatgcag cgataccggt
     2281 cttcttgttc cgcatgggcc gcgccc
//
LOCUS       DATCXU010000509         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_1001546,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000509 DATCXU010000000
VERSION     DATCXU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001546"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VIR04_11425"
     CDS             complement(<1..135)
                     /locus_tag="VIR04_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY9461399.1"
                     /translation="MNEDPPESYAQTLGKLLKDSGRMFACAESCTGGLLSAAMTHNAG
                     S"
     gene            complement(132..656)
                     /locus_tag="VIR04_11430"
     CDS             complement(132..656)
                     /locus_tag="VIR04_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY9461400.1"
                     /translation="MNTIGSNEGLSAFAAPTPAWVFRTPGRVVAFGFGSGLLRPGPGT
                     WGTALAWVLWLVVVSRFSDAAIGFILALSFLYGCWCCQKVGKELGRPDFGGMVWDEIV
                     AFWLVLWIAPGSFQAQLTAFILFRAFDIVKPAPIRYFDARLKNGFGVMWDDILAAGYT
                     LLVMAVIVRLEVLL"
     gene            complement(681..1640)
                     /gene="thiL"
                     /locus_tag="VIR04_11435"
     CDS             complement(681..1640)
                     /gene="thiL"
                     /locus_tag="VIR04_11435"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY9461401.1"
                     /translation="MFNEFDLIARYFSRAAPPGFLGVGDDCALLPVAPGMQLATSTDL
                     LLEGRHFLPDIDPNALGHKALAVNLSDLAAVGAQPLACLLGLSLPAIDHDWLSAFSDG
                     FYALADQSGCALIGGDTTRSVSGVVISVTVFGQVKAGCALLRSAAKNEDDIWVSGTLG
                     AADIALRILQNKLPFDADLLAATRPALERPMPPWRFAQKLVGVAHAALDISDGLAQDL
                     GHILTASHCGAELDYDALPLDPALAGLPAGSRQFAALSGGDVYQLCFTAPVDRRDQIQ
                     ALAATEAIRVTRVGRITSRSGLTVLAANGTRLVPVSAGFDHFA"
     gene            complement(1658..2158)
                     /gene="nusB"
                     /locus_tag="VIR04_11440"
     CDS             complement(1658..2158)
                     /gene="nusB"
                     /locus_tag="VIR04_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY9461402.1"
                     /translation="MPSKSHGAPSGTPAHRSNARSARRRAREFALQGIYAWLLRGDNA
                     DREAGEIDAHIRDSDEFQQADAQWYNVLLYGVIREAPGLRVAFAPHIDRPLAELSPVE
                     HGILLIGSYELMHHAEVPYKVAINEAVELAKSFGGTDGFKFVNGVLDKMAADLRSAEV
                     HGAAHR"
     gene            complement(2148..2684)
                     /gene="ribH"
                     /locus_tag="VIR04_11445"
     CDS             complement(2148..2684)
                     /gene="ribH"
                     /locus_tag="VIR04_11445"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY9461403.1"
                     /translation="MNPYTLEPDLIGEGLHIGIIRARFNEEIGLAELDACLAELGELG
                     VDERDVMVVSVPGALELGVTLAQMAETLEFDALIALGAVIRGETYHFELVSNESASAI
                     SRVALETGIPVANGVLTTDTDELAQVRAAPKGRDCARAAVEMANLLAALEPESDDDDD
                     EDDDASAGRDEDEDSDAE"
     gene            complement(2699..>3193)
                     /locus_tag="VIR04_11450"
     CDS             complement(2699..>3193)
                     /locus_tag="VIR04_11450"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744686.1"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II-like protein; functions in riboflavin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY9461404.1"
                     /translation="ESRPMQTPWGEFQAVAYRDLASGAPHLALVHGSISPDVEALVRV
                     HEPTSLLDVLSSGETRHSWSISQALKAISAAQSGVLVLLNCQGSAELLFEQFADWNRS
                     GEKEGTSDESRFDFRTYGIGAQILRDLNVGHARLLARPRKMPSMAGFSLTITGYDSEP
                     LNTP"
BASE COUNT          728 a         1009 c          870 g          586 t
ORIGIN      
        1 tgatccggcg ttatgggtca tggcggcgct taacaggccg cctgtgcagg attcggcgca
       61 agcaaacatt cggccgctat ccttcaatag cttgcccagc gtctgcgcgt agctttcggg
      121 tgggtcttcg ttcatagcaa cacctccagc ctgacaataa cagccataac cagcaatgta
      181 taccctgccg ccagaatgtc gtcccacatg accccaaagc cattcttgag ccgggcatca
      241 aagtagcgaa tgggcgccgg tttgacgatg tcgaacgctc tgaacaggat aaacgcggtc
      301 aactgcgcct gaaatgaacc gggtgcaatc cacaatacca gccagaatgc aacgatttcg
      361 tcccacacca tgccaccaaa atccggcctt cccaattctt tgcccacctt ttgacagcac
      421 cagcaaccgt agaggaacga caaagccaga ataaagccga tagcagcatc tgaaaatcgg
      481 gaaacgacca ccagccaaag aacccaggcc agagccgttc cccacgtgcc ggggcccggg
      541 cgcagcagac cactgccaaa gccaaaagcg acgacccggc cgggagtcct gaatacccag
      601 gccggcgtcg gcgcggcaaa cgccgataat ccttcattgg aaccgatagt attcataggt
      661 ttgcctgatg cccgttcctt ctatgcaaaa tgatcgaacc ccgccgacac cggaaccaaa
      721 cgcgtgccgt tcgctgccag aaccgtcaag ccggatcgcg atgtgatgcg cccgacgcgg
      781 gtcacccgaa ttgcctcggt tgccgccaac gcctggatct gatcccgcct gtcgacaggc
      841 gccgtaaagc ataactgata gacatcgccg ccgcttaaag ccgcaaattg cctggacccg
      901 gccggcaagc ccgcaagagc cggatcaagc ggcaacgcat cgtaatccag ctcggcgcca
      961 caatggctgg ccgtaagaat gtgtcccaaa tcctgcgcca ggccatccga aatatcaaga
     1021 gccgcatgcg ccacgcccac cagtttttga gcaaaacgcc aggggggcat gggccgctcc
     1081 agagcgggcc gcgtcgccgc cagcaaatcc gcgtcgaacg gcaatttgtt ttgcaggata
     1141 cgaagcgcga tatcggccgc gcccaaggtg ccggaaaccc aaatgtcgtc ttcatttttg
     1201 gccgcgctac gcaacaaggc gcagcctgcc tttacctgcc cgaacacggt aacgctaatt
     1261 acaacgccgg atacgctgcg ggtggtatcg ccgccgatca gagcgcagcc cgactgatcg
     1321 gcaagcgcat aaaacccatc ggaaaaggca ctgagccaat catgatcgat cgcaggcagg
     1381 gacagtccga gcaaacaggc aaggggttgt gcgcccaccg ccgccagatc ggacaaattg
     1441 acggccagag ctttgtgacc caacgcattg gggtcgatgt cgggcaagaa atgccgccct
     1501 tcaagcagca aatccgtgct ggtagccaat tgcatgccgg gcgcaacggg caataacgcg
     1561 caatcgtcgc ccacacccag aaaaccaggc ggcgccgcgc gtgaaaaata ccgtgcgatg
     1621 agatcgaact cgttaaacaa gggcctgccc gcccgcgcta acgatgcgcg gcgccatgaa
     1681 cctcggccga ccgcagatcg gccgccattt tatcgagcac cccgttaacg aatttaaacc
     1741 catcggtgcc gccaaaggat ttagccagtt ccacggcttc gttgatggcc actttgtacg
     1801 gaacctcggc atgatgcata agctcgtagc ttccgatcag caaaatgccg tgttccaccg
     1861 gcgagagctc ggcaaggggc cgatcgatgt gaggcgcgaa cgccacccgc aagcccggcg
     1921 cctcgcgaat cacgccatac agcaaaacgt tgtaccactg ggcatccgct tgttgaaact
     1981 cgtcgctgtc gcgaatgtgg gcatcgattt cgccggcttc gcgatcagca ttatcgccac
     2041 gcaacaacca cgcgtatatg ccttgcaagg cgaattcgcg ggcgcgccgc cgcgcactgc
     2101 gagcgttgga tcgatgcgcg ggcgttccgc tgggcgcacc atgggattta ctcggcatcg
     2161 gaatcttcgt cctcgtcccg cccggcactg gcgtcgtcgt cttcgtcatc gtcatcatcg
     2221 gactccggtt cgagggcggc caacaggttg gccatctcga cggcagcacg agcgcaatcc
     2281 cgccctttgg gcgcggcgcg cacctgcgcc agttcatcgg tatcggtcgt taaaactcca
     2341 ttggccaccg gaatgcctgt ttcaagcgcc acccggctga tcgccgaggc gctttcgttg
     2401 ctgacaagtt cgaagtgata ggtttcaccc cgaatcaccg cacccaaagc aataagagcg
     2461 tcgaattcaa gggtctcggc catttgagcc agcgtcacgc caagttctag cgccccgggc
     2521 actgaaacaa ccatgacgtc gcgttcgtcg acgcccagct cacccaattc ggccaggcat
     2581 gcatccagtt ccgccaggcc gatctcttca ttgaaacggg cccggataat gccgatgtgc
     2641 aagccttccc cgattaaatc aggctctaga gtgtagggat tcatagtcag cattcctttt
     2701 agggtgtatt tagcggttcg ctatcgtaac ccgtgatggt taaagagaaa ccagccatgc
     2761 tgggcatttt tctgggccgc gccaataagc gggcatggcc cacgttcagg tcgcgcaaga
     2821 tttgcgctcc aatcccgtag gtgcggaaat cgaagcggct ttcatcgctt gtgccttctt
     2881 tttcgccgga ccggttccag tcggcgaact gttcgaacaa cagttccgcc gagccctggc
     2941 aattaagcaa tacaagtacg cctgattgcg ctgctgaaat ggctttgagc gcctgtgaaa
     3001 tgctccagct atgcctggtt tcgccactgg acaatacgtc gagaagactt gtgggttcgt
     3061 gcacgcgcac caaagcttcg acatcgggcg aaatcgaccc atgcaccagc gccaaatggg
     3121 gtgccccgga ggccaggtcg cgatacgcga cggcttgaaa ctcgccccag ggcgtctgca
     3181 tcgggcgcga ctc
//
LOCUS       DATCXU010000510         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_690991,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000510 DATCXU010000000
VERSION     DATCXU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690991"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VIR04_11455"
     CDS             complement(<1..1281)
                     /locus_tag="VIR04_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY9461405.1"
                     /translation="MIILRRTQWFVPGVIACALVLLPFFGNVYLTSFMFTVLIAYVLA
                     QSWDWVAGEMGYINLGHYVFYGIGAYLFSITLVAHYPAAFAMVVATVVTAMVGLLVAV
                     PLFRLRGDYFAFATLALLPLAEILAYNLSSFTNGGDGIVLPVQNILVNAYLIAVALCM
                     VTFSTTIWLTGKRFGYALKSIRNDEEIAEMVGMRIMPVKMKALALSAAFAALAGAVQA
                     WQMSYIDPPTVFGLDIALVPIAMALFGGSGLRWGPVMGVVLLAFMQQWLLINMEILHV
                     TVFGAIILLIGRFMPGGLLRAPWIRKVSFLRGLAREHHEFIIETVNRPQIDANSLPLA
                     SAGLIKAVDPLLECRNVRMVFGGNVAVDNVNLTVMEGEIVGLIGPNGSGKTTLFNCIS
                     KVLEPTGGSIRFAGKNLSELRRDQIARLGVGRTNQ"
     gene            complement(1278..2153)
                     /locus_tag="VIR04_11460"
     CDS             complement(1278..2153)
                     /locus_tag="VIR04_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY9461406.1"
                     /translation="MSIDVIIQVLIGGILLGGLYALVAFGLSLIYGIVRVLNFAHGTL
                     LAVSGVVASLIFTNWHFHPLFIGLLIAPVVFAFGYIFYIVLIHPLERRSEFESVVGSV
                     LITVGAMLILSDVTTVLAGATQRTIPLPFESIEMGEIVVSTTQFYILVGIALLTLGMH
                     LFLKKTWFGRAIRAVTQDQTGAQICGVNSRQLKSLTFAFGSATVAIAAILYAMSFPVS
                     PYMGFPLTVKAFTIIVLGGIGNLRGALFAGIFLGVAESMTGLFWKAEWAPALSVVLML
                     VILIAFPQIAGRKAS"
     gene            complement(2234..3442)
                     /locus_tag="VIR04_11465"
     CDS             complement(2234..3442)
                     /locus_tag="VIR04_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY9461407.1"
                     /translation="MNWTKYTTTVLASSLLFISGQSMAKVGSCDDPIVFGTTISESGP
                     FSTLSDRWRKMTEVFAEEINKTGGIDVKSCHKKLPLKFVIYDDQSVPSTAVSLYERMA
                     TVDKVDFFVGPDWSSLGGPVPPIAEKHKIPMVAGNVATPSLYKRGFKYFFGTPYPEVP
                     LWSARYFDMVSKMDPKPKSIYFITQDNPVMKAITAVWSKKAADQGIKVLATETFPSEL
                     KDFTGMISKLRAAKPDIIYISSFDGASVALVQQMRHLKVHAMDVHHAMLTGSLYRQVG
                     KDIEGMTGELPWYPGVKGDYSDLIGNVLKNSNVDMFDYIFTLARLASYLTMVQAIEKA
                     GAVNREGVRDALHLGTFKSPAGNIVFDENGFPTSNGAFTLQMQGGKPKVVWPPEAATG
                     KVIWPSPTWR"
     gene            complement(3605..>4217)
                     /locus_tag="VIR04_11470"
     CDS             complement(3605..>4217)
                     /locus_tag="VIR04_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY9461408.1"
                     /translation="QSGELWINLTASLQRLFWGSLLGGIPALVIGIIMGLYRPVRVAI
                     DPLISATYPVPKSAILPLVLLIFGLGEASKIVMVALGVFYPVVINTVTGVRQIDKIYL
                     DVGKNFRAGRWQTFRGIALPGALPSIMAGMKLGIGMGLILIVIAEMVGSKTGIGFMIW
                     DAWQVLSVETMYVGLIVISILGFVLTLILNEIEGWILPWKQER"
BASE COUNT         1187 a         1190 c          940 g          900 t
ORIGIN      
        1 ctgattggtg cgacccactc ccagccgagc aatctgatct cgccgtaatt cgctcagatt
       61 ttttccagca aaacgaattg acccgccagt tggttccaga acctttgaga tgcagttaaa
      121 aagtgtcgtc ttgcccgagc cgttcggtcc gatcaagcca actatttcgc cttccataac
      181 ggttagattc acgttatcca cagccacatt tccgccaaac accatgcgca cgttgcggca
      241 ttcaagcagc ggatccaccg ccttgatcaa ccccgcagaa gcgagcggaa gactattggc
      301 atcaatttgc gggcggttta ccgtttcgat aataaattca tggtgctccc gcgccaagcc
      361 acgtaaaaag gacacctttc gaatccaagg cgcccttagc agaccgccag gcataaaacg
      421 gccgatcaat aaaataatcg cgccgaagac cgtgacatgc aggatttcca tgttgatcaa
      481 caaccactgc tgcatgaacg ccaacagcac aactcccata accgggcccc aacgcaagcc
      541 ggaaccgcca aatagcgcca tggcaatcgg caccagcgcg atatctagtc caaacacggt
      601 gggcgggtcg atataactca tctgccacgc ctgcaccgca ccagccaatg ccgcaaaagc
      661 ggcgctaagt gcaagcgcct tcatctttac cggcatgatt cgcataccta ccatctctgc
      721 gatttcttcg tcgttgcgga tgcttttgag tgcatagcca aaacgttttc ccgtaagcca
      781 gatagtcgtg gaaaaggtca ccatgcatag cgccaccgca atcagataag cgtttaccaa
      841 gatattttga accggaagaa caatgccatc tcccccgttg gtgaaagagc ttaggttgta
      901 ggcaagtatt tcggccaggg gcagcagagc caatgtggca aatgcaaagt agtctccacg
      961 caggcgaaac agaggtaccg caaccaacag accgaccatg gccgttacaa ctgtcgccac
     1021 caccatcgca aacgcggctg gataatgagc aacgagcgta atcgaaaata gataagcgcc
     1081 aataccatag aaaacatagt gtccaagatt gatatagccc atctcgcctg ccacccagtc
     1141 ccaactttgg gcaagcacat aggcgatcag caccgtgaac ataaatgacg tcagatacac
     1201 gttcccaaag aagggcagca atacaagtgc gcacgcaatc acacccggaa caaaccactg
     1261 agttcgtctc aaaataatca cgacgctttc cgccctgcta tttgaggaaa agcgatcaga
     1321 ataaccaaca tcaacacaac gcttaacgcc ggcgcccatt ccgctttcca aaaaaggccc
     1381 gtcatgctct ctgccacccc caggaaaata cccgcaaaca acgcgccacg cagattacca
     1441 atgccgccga gcacgataat agtgaacgct ttcacagtaa gaggaaaccc catatatggg
     1501 ctaactggaa atgacatcgc gtacaagatc gccgcaatgg caaccgttgc ggagccaaag
     1561 gcaaaagtta aagatttaag ttgcctgctg ttgacgccac atatctgcgc acccgtctga
     1621 tcttgcgtca ctgcacggat ggctcgaccg aaccatgttt ttttcaagaa caaatgcatg
     1681 cctagcgtca acaacgctat acccaccaat atataaaact gagtcgtcga gaccacgatc
     1741 tcgcccattt caatggattc aaatggaagt ggtatcgtgc gctgcgtagc gccagcaagg
     1801 accgtggtca cgtcgcttag aatcaacatg gcgccaaccg ttatgagcac actgcccacg
     1861 acactttcaa attcactgcg ccgctcaagc gggtgaatta acacgatgta aaagatatag
     1921 ccgaaagcga aaaccaccgg cgcaatcaat agcccgatga ataacggatg gaaatgccaa
     1981 ttggtaaaga tcaagctggc aacaacgcca ctaaccgcca gcaacgtgcc atgcgcaaaa
     2041 ttgagcaccc gaacaattcc ataaataaga gaaaggccaa acgcaactaa cgcgtacaac
     2101 ccccctaaca ggatgccccc aatcaggacc tggatgatta catcgatgga catgacaata
     2161 cgtacctcgg tagcgggact agccgccccg catttgcatc aatcgtcccg gggcggcaca
     2221 tgaccaagct tgattagcgc caagtgggcg acggccaaat gactttgccg gttgcagcct
     2281 cggggggcca cacaactttc ggcttgccac cttgcatctg cagagtaaat gctccgttgg
     2341 aggtgggaaa tccattttcg tcgaaaacga tattgcccgc tggcgactta aacgtgccca
     2401 ggtgtagtgc gtctcgcacc ccctctctat tgactgcgcc cgccttttct attgcctgca
     2461 ccatcgtcaa ataagaggcc agtctggcca aggtaaatat gtagtcgaac atatccacat
     2521 tggaattttt cagcacattt ccgatcaggt cgctgtagtc gcctttgact ccgggatacc
     2581 agggcagctc gcccgtcatg ccttctatat ccttcccaac ttggcgatac agcgacccgg
     2641 tcagcatggc atgatgcacg tccatcgcgt gcaccttaag atggcgcatt tgctgtacca
     2701 gtgcaacgga agctccatca aacgagctaa tgtaaataat gtcgggcttt gcggcgcgaa
     2761 gttttgaaat cattcccgtg aagtccttca actccgatgg aaaggtctct gttgcaagaa
     2821 ctttgatccc ctgatccgcc gccttcttgg accagactgc tgtaatggcc ttcatcacag
     2881 ggttatcttg ggtaatgaaa taaatggatt tcggttttgg atccatcttc gacaccatgt
     2941 cgaaataacg cgcggaccaa agcggaacct cggggtaagg tgtgccgaag aaatacttga
     3001 agccacgctt ataaagcgac ggagtggcca cgttgccagc caccattgga attttgtgtt
     3061 tctcggctat aggcggaaca gggcctccga gtgacgacca gtcggggcca acaaagaagt
     3121 ccaccttgtc gacagttgcc attcgctcat acaacgacac cgcagtagac gggacacttt
     3181 gatcatcata tatgacgaat ttcagcggaa gttttttgtg acacgattta acgtctatgc
     3241 cgccagtctt atttatttcc tcagcgaaaa cttcggtcat tttgcgccat cgatccgaca
     3301 atgtcgaaaa gggacccgac tccgaaatcg tggtgccgaa aacaatcggg tcgtcacatg
     3361 atcccacttt tgccatcgac tggcccgata taaacagcaa tgagcttgca agaacagtcg
     3421 ttgtatattt cgtccaattc atattcatgt ctcctcataa atcaacttcg atcagccatg
     3481 ataggtagtc aattaaaact tggtgtggcg ttccatcaac attctcgctc aagaccatca
     3541 tagtgactcg gagggcaata acctatatca acggatccga cgatttaccc taaagtctct
     3601 ttatctatct ctcctgcttc cagggtaata tccacccctc gatttcgttg aggataagcg
     3661 tgaggacgaa ccccaaaatg gaaatgacta tcaaaccgac atacatagtc tctacagaaa
     3721 gcacttgcca tgcatcccaa atcatgaatc ctattcccgt cttcgatcca accatttcag
     3781 caataacgat cagaatcaaa cccatgccaa ttccaagctt catacccgcc atgatggaag
     3841 gcaatgcgcc gggaagagcg ataccgcgaa atgtctgcca tcggcccgcc cgaaaattct
     3901 ttccaacatc cagataaatt ttgtcgattt gccgtacgcc ggtgaccgtg ttgattacga
     3961 ccggataaaa cactcctagc gccaccatga caattttgga agcttccccc aagccaaaaa
     4021 tcaggagaac taacggcagg atcgcgctct ttggaacggg ataggtggcg gaaatcaaag
     4081 gatcaatcgc aacacgcacc ggtcggtaaa ggcccatgat gataccaatc accaaagcgg
     4141 gaatacctcc caacaatgaa ccccaaaata agcgttgcag gctcgcggtc aggttgatcc
     4201 aaagctcgcc cgactgg
//
LOCUS       DATCXU010000511         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_534361,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000511 DATCXU010000000
VERSION     DATCXU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534361"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VIR04_11475"
     CDS             complement(<1..1017)
                     /locus_tag="VIR04_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY9461409.1"
                     /translation="MSSTPRNIDHLDARALRALAGDLMVELGEREQALAEHRQLLVRQ
                     EQMLARHRNDIRYKDAKIAQLTHEIAGLRRFTFGKRGEQLSGAQGSLLEETVCADIAA
                     IEVELDLLRGEPAPRPAQQPKRAALPEHLPRVEVRHEPDSATCQCGCQLQRIGEDVSE
                     KLDYTPGLFTVERHIRGKWICKKCATLIQAPVPAHVIDKGMATTGLLAQVLVNKYADH
                     LPLYRQEKIFERAGMKLSRSTLAQWVGACGVQLQPMVDALREAILAHRVVHADETPVQ
                     MLKPGDKKTHRAYLWAYAPGAFEDLRAVVYDFTEGRAGEHARAFFGDWQGKLICDDYV
                     GYKKS"
     gene            complement(1098..1460)
                     /gene="tnpB"
                     /locus_tag="VIR04_11480"
     CDS             complement(1098..1460)
                     /gene="tnpB"
                     /locus_tag="VIR04_11480"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY9461410.1"
                     /translation="MRGLAAPVAGVIRVDQIWLAVEPMDMRAGTDTALARVVKVFGAA
                     RPHHAYLFANRRGNRLKVLVHDGVGIWLAARRLNRGRFVWPRGDCVNVELSAEQLQAL
                     VLGLPWHRIGPGGVIDIL"
     gene            complement(1427..1801)
                     /locus_tag="VIR04_11485"
     CDS             complement(1427..1801)
                     /locus_tag="VIR04_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY9461411.1"
                     /translation="MEITPTLRRPPRRLYTKEFKAQRVKECAEPGASVARVALVHGIN
                     ANLVHKWRRQSERGELGVGAAPEFLPVALKRVAALPTPGMTAQAGSQVRIDLAGRNLT
                     ASVHWPVSHAAQCAAWLRQLLA"
     gene            complement(2211..2507)
                     /locus_tag="VIR04_11490"
     CDS             complement(2211..2507)
                     /locus_tag="VIR04_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY9461412.1"
                     /translation="MSRIELVSEVGEDFDRILDHLAKYQVENSALRIQEIVEALNVLE
                     HNPLIGRPVNNDKRELVIGRRSHGHVALYRYIVEIDTVFVLAVRSQREAGYTSL"
     gene            complement(2510..2794)
                     /locus_tag="VIR04_11495"
     CDS             complement(2510..2794)
                     /locus_tag="VIR04_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY9461413.1"
                     /translation="MSTTTIRLPEDLKARVAAAAKRSGTTAHAFIIEAIVEKTGQEDL
                     RADFDAVAEDRYAGIVASGKTIPWDEMRGYLQDRLAGKVVKRPSARKLAR"
     gene            complement(3083..3271)
                     /locus_tag="VIR04_11500"
     CDS             complement(3083..3271)
                     /locus_tag="VIR04_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEY9461414.1"
                     /translation="MPAKSKAQQKAAGAALSAKRGDTKVTKLKGASKSMYKSMSEKQL
                     DELASTPRKGKPEHKSGS"
     gene            3448..>3954
                     /locus_tag="VIR04_11505"
     CDS             3448..>3954
                     /locus_tag="VIR04_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY9461415.1"
                     /translation="MTATANFPVVGIGASAGGLAAFEAFFSGMPAGVEPRMAFVVVQH
                     LAPDHKSILAELIRRRTRMQVFEVEDGVVVKPNCVYIIPPDRDMAFLSGTLQLLEPSA
                     PRGHRLPIDFFFQSLARDQRERAICIVLSGTGGDGALGVRAVKNEGGMVMVQSPDSAE
                     FDGMPNSAI"
BASE COUNT          793 a         1204 c         1115 g          842 t
ORIGIN      
        1 ggattttttg tagccgacat aatcgtcgca aatgagtttg ccttgccagt cgccgaagaa
       61 ggctcgggca tgttcgcccg cgcgcccttc ggtgaagtca tacaccacgg cgcgcaagtc
      121 ctcgaaggcg cccggggcgt aagcccacag gtaggcgcga tgcgtcttct tgtcgcccgg
      181 tttgagcatt tgcacgggtg tctcgtcggc atgcaccacg cggtgggcga gaatggcctc
      241 gcgcaacgca tccaccatgg gctgcaattg cacgccacat gcgcccaccc attgggccaa
      301 cgtggagcgc gacagcttca tgcccgcccg ctcgaagatt ttctcctgcc ggtacagcgg
      361 cagatgatcc gcgtatttat tgaccagaac ctgggccaac aacccggtcg tggccatccc
      421 cttgtcgatg acgtgggccg gcaccggcgc ctggatgagg gtcgcacact ttttacatat
      481 ccatttgccc cggatgtggc gttcgacggt aaacagcccg ggcgtgtaat cgagcttctc
      541 ggacacgtct tcgccgatgc gctgcaactg gcagccgcat tgacaggtgg ctgaatccgg
      601 ctcgtgacgg acctcaacgc ggggcagatg ctcgggcagc gccgcgcgct tgggttgctg
      661 agccggccgg ggggcgggtt ccccgcgcag caggtccagc tccacctcga tggcggcgat
      721 gtcggcgcaa accgtttctt ccagcaggct gccttgcgcg ccgctgagtt gctcgcccct
      781 cttgccgaag gtaaaccggc gcagcccggc gatctcatgc gtcaactgcg cgatcttggc
      841 gtccttatag cggatgtcgt tgcggtggcg cgccagcatc tgctcctggc gcaccaggag
      901 ttgccggtgt tcggccaacg cctgctcacg ctcacccagt tcaaccatca ggtcgccggc
      961 cagcgcgcgc agggcccggg cgtccaagtg gtcgatgttg cgcggcgtgg atgagatcat
     1021 gggcctaagt ttgccaccgg actcgcgttc tcgccattgg cgcatgcccg aattgccagc
     1081 gtgcgcaact tcagaaacta taggatatcg atgacgcccc ccgggccgat gcggtgccac
     1141 ggcaagccca gcaccagcgc ctggagttgc tcggcactaa gctcaacgtt gacgcaatcg
     1201 ccgcgcggcc agacgaagcg cccccggttc aggcggcgcg cggcgagcca aatgcccacg
     1261 ccatcatgca ccagcacctt caggcgattg ccccggcggt tggcgaacaa gtaagcatgg
     1321 tgcggtcggg cggccccgaa caccttgacc acccgcgcca gggccgtgtc ggtgccggcg
     1381 cgcatgtcca tcggttccac tgccagccag atctgatcga cgcggatcac gccagcaact
     1441 ggcgcagcca ggccgcgcac tgcgccgcat ggctgacggg ccagtgcacc gaggccgtta
     1501 aattgcgacc agcaaggtcg atgcgaacct gggatccggc ctgcgccgtc atgcctggcg
     1561 tgggcaaagc cgctacccgt ttaagcgcga ccggcaagaa ttccggcgcg gcccccacgc
     1621 cgagttcacc gcgttcgctt tgccggcgcc atttatgcac cagattggcg ttgatgccgt
     1681 gcaccagcgc cacccgggcc accgaggcgc caggctcggc gcattccttg acccgctgcg
     1741 ccttgaactc tttcgtataa agacgcctcg gcgggcgtcg cagcgttgga gtgatttcca
     1801 tagtgtccac cgtatttggg gtggacatca tctcctagtg actgaagcga ctcaagatgg
     1861 gattaccgga cgcttacccc cacatacgat gaaacattca tcatttgcga aggacactac
     1921 gactttcagc ttgataaaaa gatgctcaag ttgggtccag gggatacggt gtttgttccg
     1981 agaggcactc ctcacagctt tatttctatc ggccccgaaa tcggacgtca actaatcatc
     2041 agttccccgg gcggtatctt cgatgcgttc attgccgaaa tagccacgtc acaggccgat
     2101 agcgggagtc cctcgaagcc ggaggcagca acgaatttca gatccattgc ggcgaagtat
     2161 ggaatgcagt ttctgagcta gaggagcaca ggaggaatag ccccagcccc tcagagcgac
     2221 gtatagccag cctcccgttg gctacgtact gcaaggacaa aaacggtatc gatttcgaca
     2281 atgtaccggt acaacgccac atggccgtgc gaacgacgcc ctatcaccaa ttctcgctta
     2341 tcgttattta ctgggcggcc aatcaaggga ttgtgctcta gcacgttgag cgcctcaacg
     2401 atctcttgaa tgcgcagcgc tgaattttcg acctgatact tggccaagtg atcgaggatg
     2461 cgatcaaaat cctcccccac ctctgaaacc aattcaatac gtgacatttt caacgcgcca
     2521 gttttcgagc ggagggacgc tttaccactt tgccggcaag gcggtcttgc aggtagccac
     2581 gcatttcgtc ccacggaata gtctttcctg atgcaacgat acccgcgtag cgatcctcgg
     2641 caacggcatc gaaatcagca cgcaaatctt cctgccctgt cttttccaca atggcttcaa
     2701 taatgaaggc atgagcagtt gtacccgaac gtttggctgc tgcggcaacg cgggctttca
     2761 ggtcttcggg caaacgtatc gtcgtcgtgg acatggctat ctccagggat taaagctatc
     2821 ctgtctatgt agcacaaaag tgctacatta atctatatct ctttggggaa aactgacaaa
     2881 gacatcagcc taaaacaatc aatagtcaga acggccgaaa gccccaatcc acgcccttct
     2941 acccctttcc tgcccatcgt gttctgagaa ccatttcagg gttagtgccc ctactccacc
     3001 accagttagt gtttttccta ataaatcagt aagttgcatg acggccgagt aacggggtca
     3061 ggttcgaaaa cgggtccctg cgctacgaac cggacttgtg ttctggtttg cccttgcgcg
     3121 gcgtcgaagc caattcatcg agctgcttct cgctcatgga tttgtacatg gatttcgatg
     3181 cgcctttaag cttggtgact ttggtgtcgc cgcgcttagc tgaaagcgcc gcgcctgccg
     3241 ccttctgttg agcttttgat tttgctggca taatgaacct cctgataggc tcacgttaat
     3301 tgacgttttg caccaatctc attattcacg taaatcgtcg aggcttcaac catcaacaga
     3361 gattctggaa cctcgcatga ctgaaaaaaa agacaaactg ccgcggcggc gcacttgcca
     3421 gacggcgcca aggcggctaa cgcaccggtg acggctactg cgaattttcc ggttgttggc
     3481 atcggcgcat cggcgggagg tttggcggcc ttcgaggcct ttttctctgg catgcctgcg
     3541 ggcgttgaac ctcgtatggc gtttgtggtg gtgcaacatc tggcgccaga ccacaaaagc
     3601 attctcgctg aactcatccg gcgccgcacc cgcatgcaag ttttcgaggt cgaagatgga
     3661 gtggtcgtta aacccaattg cgtctatatc atcccacctg accgcgatat ggcgtttctg
     3721 agcggaacgc tgcaattact cgaaccttca gctccgcgcg gccatcgttt gccgattgac
     3781 ttcttctttc agtcgctggc gcgtgaccag cgcgagcgcg ccatctgcat cgtgctctca
     3841 ggcactggcg gcgacggtgc gcttggcgtg cgagccgtca agaacgaggg tggcatggtc
     3901 atggtgcaaa gcccagactc cgccgagttc gatggcatgc cgaacagcgc catc
//
LOCUS       DATCXU010000512         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_601808,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000512 DATCXU010000000
VERSION     DATCXU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601808"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1119)
                     /gene="prmA"
                     /locus_tag="VIR04_11510"
     CDS             complement(184..1119)
                     /gene="prmA"
                     /locus_tag="VIR04_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY9461416.1"
                     /translation="MRELVLTCLQAHAEALSDALFAAGALSVSFEDADSDTEGEQALF
                     GEPGTEPEIQAWQRNRVVALLPDGADPQQVIEQVRACGTDICGDGQWHVQKVPDEDWV
                     RLTQAQFGPIQIGERIWIVPSWHRDDPAVPRVAVGKTIIDAAGGPIRIDLDPGLAFGT
                     GSHATTRLCLEWLAANLRSGDTVLDYGCGSGILAIAAKMLGSGRVWAVDVDEQAVQST
                     RYNAQVNHVVLDALLPDELPAGQVALVLANILSNPLKVLAPVLANRVASGGWLVLSGV
                     LEHQADEVARAYSPWLPLSVWRAQAGWVCLHGQRV"
     gene            complement(1181..>2024)
                     /locus_tag="VIR04_11515"
     CDS             complement(1181..>2024)
                     /locus_tag="VIR04_11515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814951.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY9461417.1"
                     /translation="MRVVYTEAALFNAVAMTRSEAGIAFNNPEVYMEKFLENPRHVEI
                     QVLADGGRNAIWLGDRDCSMQRRNQKIIEEAPAPGIPRRVIERIGERCAEACRKIGYR
                     SAGTFEFLYENEEFFFIEMNTRIQVEHTVTEAVTGIDLVQQQILIAAGEKFLLRQRDV
                     QIKGHALECRINAEDPYKFTPSPGRITDWHVPGGPGVRMDTHVFPGYVVPPYYDSLIA
                     KLVTYGDTRAQAIARMDIALSEMVVDGVLTNIPLHRELMRDARFIEGGTSIHYLEHKL
                     SQRP"
BASE COUNT          477 a          631 c          513 g          403 t
ORIGIN      
        1 ccagtagcgg tagccgcaga accgccaggc acaaccgcct cgtagccatc aaagatatgg
       61 gcacaattgg tgcaacgaat aaagcccttg cgcaattgca actgttccag acttgccgag
      121 aacgttgaac cacaatttgg gcaccgcgtg gttaagtcca taaacgaaaa tcaatcttgg
      181 ccattaaacc cgttgcccat gcaggcaaac ccaacccgcc tgagcacgcc acacgctcag
      241 cggcagccat ggcgagtatg cacgcgccac ctcatcggcc tgatgctcga gcacgccgga
      301 taaaaccagc caaccgcctg aagcaacgcg attggcgagc acaggagcaa gcactttcag
      361 cgggttggac agtatgtttg caagcaccag cgccacttgc ccggcaggca gttcatcagg
      421 caacagggca tccagcacca cgtgattcac ttgtgcattg tagcgagtcg actgcacagc
      481 ctgctcgtca acatcgacag cccagacccg gcccgaaccc agcattttag ccgctatggc
      541 caaaatgccc gacccgcaac cataatcaag cacggtgtcg ccagaccgaa ggttggccgc
      601 cagccactcc agacacaaac gcgtggtcgc atggctgccc gtcccaaagg ccaagcctgg
      661 atcgagatcg atccggatgg ggccgcccgc cgcgtcgatg atagtttttc ccacggcaac
      721 gcgcggcaca gccggatcat cgcgatgcca gctcggcact atccagatac gctcgccgat
      781 ttgaataggg ccaaactgag cctgagtcag acgcacccag tcttcgtcgg ggactttttg
      841 cacatgccat tggccgtcac cgcaaatgtc agtcccgcaa gcgcgcacct gttcgattac
      901 ctgctgagga tcagcgccat cgggcaacag cgcgacaacg cgattgcgtt gccatgcctg
      961 tatctccggc tctgtgcccg gctcgccaaa taaagcttgt tcaccctcgg tgtcgctatc
     1021 ggcgtcctcg aatgaaaccg atagtgcccc cgccgcaaac aaggcgtccg acaacgcctc
     1081 ggcgtgggcc tgcaggcaag taagcacgag ttcacgcata gtattttcca ttcaccttcg
     1141 ccgtcgtgac taactgaaag acacaacggg cgccaggcgt ttacgggcgt tgcgacagct
     1201 tatgctccag ataatgaatg ctcgtgccgc cttcgataaa gcgtgcatcg cgcatgagct
     1261 cccgatgcaa aggaatattg gtcaatacgc catcgacaac catttccgac aatgcaatgt
     1321 ccatacgcgc aatcgcttgc gcgcgcgtgt cgccatacgt gacaagcttc gcaatcaaag
     1381 aatcataata tggcggaacc acatagcccg gaaaaacatg tgtatccatg cgcacgccgg
     1441 ggccgcccgg gacatgccaa tcggtaatac gccctgggct aggcgtgaat ttgtacggat
     1501 cctcggcatt aatccggcat tccagagcgt gccccttgat ttggacgtcg cgttggcgca
     1561 ataaaaactt ttcgccggct gcgatcagga tctgttgttg caccagatcg atgccggtca
     1621 ccgcctcggt gaccgtatgc tcgacctgaa tccgggtatt catttcaata aaaaagaatt
     1681 cctcgttttc atagagaaat tcaaacgttc ccgcgctacg gtagccaatt tttcggcatg
     1741 cctcggcgca gcgctcgcca atacgttcga taacgcgcct tgggattccg ggggccggag
     1801 cctcttcaat gattttttga tttcgccgtt gcatggagca atcacgatcg cccagccata
     1861 ttgcattgcg cccgccatca gccagaacct gaatttccac atgtcgcgga ttctcgagga
     1921 acttctccat atagacttcg ggattgttaa acgcaattcc cgcctcggag cgcgtcatgg
     1981 ccaccgcgtt aaaaagcgcc gcttccgtat acaccacgcg catg
//
LOCUS       DATCXU010000513         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_770717,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000513 DATCXU010000000
VERSION     DATCXU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770717"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VIR04_11520"
     CDS             complement(<1..661)
                     /locus_tag="VIR04_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY9461418.1"
                     /translation="MSSITADHIPTSQAHDAHATPHGWRRWLFATNHKDIGTMYLIFA
                     FCMFLEGGILALLLRTELFEPGIQFFEPELFNQFTTMHGLIMIFGAIMPAFVGFANWM
                     VPLQIGASDMAFARMNNFSFWLLPVGAILFTASFFMPGGAPAGGWTMYAPLSLQMGIG
                     MDFIIFGVHILGISSIMGAINIIVTILNLRAPGMTMMKLPLFCWSWLITAYLLLAVMP
                     AL"
     gene            complement(748..1908)
                     /gene="coxB"
                     /locus_tag="VIR04_11525"
     CDS             complement(748..1908)
                     /gene="coxB"
                     /locus_tag="VIR04_11525"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY9461419.1"
                     /translation="MKKLRGLLGIGAWLLMSAAGAQVKDMPGGPKVNQLNLPEGVTQI
                     ARDIMWLHWMILIICTVIFIGVFGVMIYSIIMHRKSRGYKASGFHENTAVEIAWTVIP
                     FMIIISMALPATRTVVAMKDTSSADMTIKVTGYQWKWGYDYVDGPARGVSMLSTLSTP
                     QDQIEGRAPKDNTYLMEVDKPLVVPVNKKVRVMVTAADVIHSWMVPDFGVKQDAIPGF
                     LRDVWFRAEKIGTYRGQCAELCGKNHAFMPIVVQVVSDADFEKWAAKEQKSLLASADD
                     PNKKWSKTDLMARGEKVFAANCVACHQANGKGIPGTFPALDGDKKFVLAPMKGQILTV
                     LNGHPGTAMAAWRDQLSDVEVAAAITYTRNAWSNAGSGPDPVVQPTDVKALR"
BASE COUNT          544 a          640 c          656 g          504 t
ORIGIN      
        1 ccagggcggg catgacggca agcagcaggt acgcggtgat tagccaggac cagcagaaca
       61 aaggcagctt catcatcgtc atgccgggtg cgcgcagatt caggatggtc acaataatgt
      121 ttatggcccc catgatcgac gagatcccga gtatgtggac tccaaaaatg atgaagtcca
      181 tgccgatgcc catttgcagc gacaagggcg cgtacatggt ccagccgccg gccggcgcgc
      241 cgccaggcat gaagaacgag gccgtgaaca agatggcgcc cacaggcaag agccagaagc
      301 tgaaattatt catgcgcgcg aaggccatgt cggaggcgcc gatttgcagc ggaaccatcc
      361 aattggcaaa gccgacgaag gccggcatga tcgcaccaaa gatcatgatc agaccgtgca
      421 tggtcgtgaa ctgattgaag agctcgggtt caaagaactg aatgccgggt tcgaatagtt
      481 cggtgcgcag caatagcgcc aggatgccgc cttccaggaa catgcagaac gcaaagataa
      541 ggtacatggt cccgatatct ttgtgattgg tggcaaacag ccagcgccgc cagccgtgag
      601 gcgtagcgtg ggcgtcgtgc gcctggctgg tggggatgtg atcggcagta atgctgctca
      661 tggtggactc cttcctactt gaccaaggcg ccgcaaaacg gcggccttgg caaaaaacct
      721 gtggattgtt tatacacgtt ggactgctta gcgcaaggct ttgacgtcag tgggctggac
      781 cacagggtcg gggccgctgc ccgcattgct ccaggcgttg cgtgtatagg taattgccgc
      841 tgcaacttca acatcgctta attggtcgcg ccacgccgcc atcgcggtgc ctgggtggcc
      901 gttcagtacc gtaaggattt gccctttcat gggagcaagc acaaatttct tgtcgccatc
      961 aagcgccggg aacgtgccgg ggatgccttt gccattggcc tggtggcagg ccacgcaatt
     1021 ggcggcaaac actttttcgc cacgcgccat aaggtcagtc ttggaccact ttttgttggg
     1081 gtcgtcggca gaggccagca agcttttctg ttctttggcg gcccactttt caaagtcggc
     1141 atcggaaacg acctggacga caataggcat gaaggcgtga tttttgccac agagttcagc
     1201 acattggccg cgataggtgc cgattttttc ggcgcggaac cagacatcgc gcaagaagcc
     1261 cgggatggcg tcttgcttga cgccaaagtc gggcaccatc cacgagtgaa tcacgtcggc
     1321 ggcggtaacc atgacgcgca cctttttatt gactggcacc accaaaggct tgtctacttc
     1381 catcaggtag gtgttgtctt tgggcgccct gccttctatt tgatcttgcg gtgtggagag
     1441 cgtggacagc attgagacgc cgcgcgccgg gccgtcgaca tagtcatagc cccacttcca
     1501 ttggtagccc gtcaccttga tggtcatgtc cgcgctggat gtgtctttca tggcgacaac
     1561 ggttctggtc gccggcaagg ccatgctgat aatgatcatg aacggaatga ccgtccatgc
     1621 gatctcaaca gccgtgtttt cgtgaaaacc cgaagcttta tacccacgcg acttgcggtg
     1681 catgatgatg gaataaatca tcaccccgaa aacgccaatg aaaatgacgg tacagatgat
     1741 gaggatcatc cagtgcagcc acatgatgtc gcgcgcgatt tgtgtcaccc cctcggggag
     1801 gttaagctga ttgactttgg ggcccccggg catatcttta acctgcgcac cagccgcgct
     1861 cataagcagc cacgcgccaa tgcccagtaa ccctctcaac ttcttcatgc tgacctcgaa
     1921 atacgacgtg ttttcaccaa atgccgatcc gaaccgatca tggattcagt ctggaaatcg
     1981 ttgggaacgc acaattatta gacggcaggt ttagaattgc aacgcctcgc ccaccctgct
     2041 cgaattaccc ggagacggcg gaattataac gaactgaata gaattgtgcc tcattacatc
     2101 ttcgttcgaa agggttgcgt tccataaacg ctgaataacg aagtaccggc atacactaca
     2161 atctttccat gaatgaacca catttatatc cgttgatcac cgcaatcaat caacgttatc
     2221 aacagcttgc acgacgcgtc caacaattgc caccggctgg atcgcgtccg ctcaccgtcg
     2281 cgggaaaagt atcgggctgg gttacaccga aggcaacgcg cgcattggtc gacctgcctg
     2341 gcgt
//
LOCUS       DATCXU010000514         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_292051,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000514 DATCXU010000000
VERSION     DATCXU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292051"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VIR04_11530"
     CDS             complement(<1..429)
                     /locus_tag="VIR04_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY9461420.1"
                     /translation="MVELEDQDLAMVQLGRLLKSSGYCFTTVTPKTHALVNARRGNEW
                     ARGLKGVFGWSRPFRTQTVPVEILNAMHAAGVVQSHDDGWRSTVRVSTLNGNLYFHSP
                     YPTTEANAVFFGPDTYRFVNAIDRYLATGCTVHRAVDIGCG"
     gene            complement(422..1834)
                     /locus_tag="VIR04_11535"
     CDS             complement(422..1834)
                     /locus_tag="VIR04_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY9461421.1"
                     /translation="MESIENTVLTPSCSEARQIYFGLLCDEPDKVVVERSRSYLVELL
                     QMAARNEADLPSNPDGLAAWSTSNAVNVGAQYREYLDARKAGGARRYFGTKSHALYFL
                     RSVAPTKLVDGVWLYGLLRHWGDTRFSPLIQTYLEELGRGLPDKNHVVVYKKLLAAHG
                     CEQWDTLSDDHFVQGTIQLTLAQHAANFLPEVIGFNLGYEQLPLHLLICAYELNEVGI
                     DPYYFTLHIGVDNVVSGHGAKAIQGLFDALPHIADKRNFYQRVVNGYKLNSMGTGTNS
                     VIAQFDLYKELVSILAAKCCVGAHLHSDYGRIAGKSVNQWLSDSAQLPAFLDSLQQTG
                     WIKRHQDPKHSRFWNLIQGEHAEMFGVFNAYERQVIADWIAGDSIQGQMRHPLSYKDR
                     RYLYNVLPTRKDASYTGEGAESCDDFNMELRLLENKLGDLSDKDATMATLVKLMSPVH
                     HHTDVGLMATRVFTRLLDNG"
     gene            2142..2699
                     /gene="mug"
                     /locus_tag="VIR04_11540"
     CDS             2142..2699
                     /gene="mug"
                     /locus_tag="VIR04_11540"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEY9461422.1"
                     /translation="MTRVDSSQPLPDVIAMDLTIVFCGINPGMSAAAAGHHFVGRSNR
                     FWRVLHLAGFTPEQIRPEYDRSLLEYGCGLTTAVERPTTRADELSNDEFTSASEKLAR
                     KIGQYAPRYVAFLGKVPFSVISGQRKVQWGPQAARFGGASVWVLPNPSGLNRAFKLDD
                     LVQAYRALRVAANSGSAPLYAHAMV"
     gene            2798..3952
                     /locus_tag="VIR04_11545"
     CDS             2798..3952
                     /locus_tag="VIR04_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY9461423.1"
                     /translation="MPGLLPDVDPDGLLEYSVVYTDRAINHMSGVFQGVMKDISRTLK
                     KVYNAESAIVVPGSGTFGMEAVARQFATGKKCLVIRNGWFSFRWSQIFDAGHIPAETI
                     VLKARTVEAGNQAAFAPAPIKEVVAAIRQHKPDVVFAAHVETASGILLPDDYMRQIAQ
                     AVHAEGGLFVLDCIASGTLWVDMKAIGVDILISAPQKGWSGPACCALVMLNASARKKM
                     ETTTSTSFACDLRKWLQIMEAYEKGGHAYHATLPTDSLTALRNVMAEAEAYGFEKVRA
                     EQQELGDKVRALLRKKGFKSVAAPGFEAPGVVVSYTSDDGIHSTKSFVQAGLQAAAGV
                     PLQCDEPQGFKTFRIGLFGLDKLHNVDRTVATLEKALDNITQREPEKALQ"
     gene            complement(3964..4848)
                     /locus_tag="VIR04_11550"
     CDS             complement(3964..4848)
                     /locus_tag="VIR04_11550"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY9461424.1"
                     /translation="MSVVAFVGLGAMGLPMARNLLSKGFEVRGWDLSDKALDALAQSG
                     GVRVTSLADATRGADVLVLMVVNASQAESILVQQGVLDGLAANAVVCLMATCPSGAVQ
                     NLSKSVASANRRFVDAPVSGGVAGAVAGSLTIMAAAERASFDAVEPVFNALGERVFYI
                     GPTPGQGAMVKTVNQLLCGVHIAVMAEAFSLAAKAGIDLELLLEIMQGSAASSWMLHD
                     RGPRMLQDDPEVTSTVDIFVKDLGIVLETGKDARAALPIAAAAAQLFLAASGRGDGLA
                     DDSQVIRSYFALNNARTK"
     gene            complement(4916..>5075)
                     /locus_tag="VIR04_11555"
     CDS             complement(4916..>5075)
                     /locus_tag="VIR04_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEY9461425.1"
                     /translation="VASLLLQSGVAPRFRLAGLPDAFLDAGALPTLHDRYGISTEALG
                     ERIRQWLG"
BASE COUNT         1155 a         1479 c         1371 g         1070 t
ORIGIN      
        1 gccgcagcca atatcaacgg cgcgatgcac tgtgcaaccg gtcgccagat agcggtcaat
       61 ggcgttcaca aaccgatagg tatccggccc gaagaaaacg gcgtttgctt cagtcgttgg
      121 gtaaggtgaa tgaaaataga ggttgccatt taaggtcgaa acgcgcacgg tgctgcgcca
      181 gccatcgtcg tgtgattgca ccacgccagc cgcatgcatg gcattcagga tttcaaccgg
      241 cactgtctgc gtgcgaaacg ggcggctcca gccaaaaacg cctttcaggc cgcgcgccca
      301 ttcattgccg cgccgagcgt tcaccagtgc atgggttttc ggtgttacgg tcgtaaagca
      361 atagccgcta cttttcaaaa gccggcccag ttgcaccatt gcaaggtctt ggtcttcgag
      421 ttcaaccatt gtcgagcagg cgcgtgaaga ctcgagtagc catcaaaccg acgtcggtgt
      481 ggtgatgtac tggggacata agtttaacca atgttgccat tgtggcatcc ttgtcggaca
      541 gatcgcccaa cttgttttcc agtaaacgca actccatgtt gaaatcatcg cacgactccg
      601 cgccttcccc cgtgtatgaa gcgtctttgc gagtgggcaa cacgttgtat aaatatcgtc
      661 tgtccttgta gcttaatgga tgccgcattt gaccctggat actgtcgccg gcaatccagt
      721 cggcgattac ttgcctttca taagcattga atacgccgaa catttctgcg tgctcgccct
      781 gaatcaagtt ccagaagcgg ctgtgcttgg ggtcctgatg gcgcttgatc cagccagtct
      841 gctgcagact atccagaaag gcgggaagct gggcggaatc agacagccat tgattgaccg
      901 atttgcccgc gatccggcca tagtcggaat gcaggtgcgc tcctacgcag catttggcgg
      961 caagaataga cactagctct ttgtacaaat cgaactgcgc tatgaccgaa ttagtgcccg
     1021 tacccattga attcagctta taaccattga caacacgctg atagaagttc cgtttatccg
     1081 caatatgggg cagcgcgtca aacaagcctt gtatcgcctt ggctccgtgc ccggaaacga
     1141 cattatcaac gccgatatgc agagtgaaat aatagggatc aatgcccact tcgtttaatt
     1201 cgtaagcgca aatcagcaaa tgcaagggca actgttcata acccagatta aagcctatga
     1261 cttccggcag aaaattggcg gcatgttgcg ccagcgtcaa ctgtattgtt ccttgcacaa
     1321 aatggtcatc gcttaatgtg tcccactgtt cgcaaccgtg tgcggcaagc agttttttat
     1381 aaacgacgac gtggtttttg tcgggaagcc cccttcccag ttcttccaga tatgtttgaa
     1441 tcagcggcga aaagcgcgtg tcgccccagt gcctgagcaa gccgtacagc cagacgccat
     1501 caaccagctt ggtcggcgcc accgatctta aaaaatacaa ggcatgcgat ttagtgccaa
     1561 agtaacgcct tgcacctccc gcttttcgcg catccaggta ttcgcggtac tgcgctccca
     1621 catttacggc attagaggtg ctccaggcgg caagaccgtc cgggtttgac ggcaagtcgg
     1681 cctcatttcg agccgccatt tgcaacaatt cgactaaata tgaacgcgaa cgctctacga
     1741 caaccttatc tggttcgtcg cacaagaggc cgaaataaat ctgcctggcc tctgaacatg
     1801 aaggggtcag aaccgtattt tcgatgcttt ccatttcgat tctcgctatt ttgtcagtgg
     1861 caacttctag tcgatagctg accaaccgca gcggaacagc gctgccccgg tttcgttcct
     1921 tttccccaat gatcgttcct gaatgccgct tccgtgaggt attcgaaccc gcgaataagg
     1981 gcgcagcaat ccgccaaacg gatggaacat ttagaaaaac aacagtgtta aaacacttcc
     2041 aaatgacgca caaagttgca tcaggcatcg attgacggtg cgacgtctgc accgcaaaca
     2101 gaatccggag ccggcttgcg tgcatcgcgt atgattgccg gatgacacgc gtcgattctt
     2161 ctcaaccgct gcccgacgtt atcgccatgg acctgacgat cgttttttgc ggaatcaacc
     2221 ctggcatgag tgccgcggcg gccggccacc atttcgtcgg aagaagcaat cgcttctggc
     2281 gtgtattgca cctggcgggg ttcacgcccg aacaaatacg ccccgaatac gatcgcagtc
     2341 tcttggagta tggctgcggg ctgacgacgg ccgtcgaacg tccgaccaca cgcgctgacg
     2401 aactttcgaa cgatgaattc acatccgcat ctgaaaaact ggcgagaaag atcgggcagt
     2461 atgcgccgcg ctacgtcgcc ttcctgggca aagttccctt ttccgtaatt tccgggcaaa
     2521 ggaaagtcca gtgggggccg caagccgccc gtttcggcgg cgcgtcggtg tgggttctgc
     2581 caaatcccag cgggctgaac cgcgcattca agcttgacga cctggtgcag gcctatcgtg
     2641 cgcttcgtgt tgcggcgaat agcgggtctg caccgctata tgcccacgca atggtctaaa
     2701 atcagtccag gcgctgctaa cttggcggcg ccgtggcaag tcgaagattc ctgcgtgcaa
     2761 acgcggtgtg ttcgcacgat tttttctgga gaacaatatg cctggcctat tgcctgatgt
     2821 cgaccctgac gggctacttg aatactcggt cgtctatacc gaccgcgcca taaaccacat
     2881 gtctggcgtt ttccagggag tgatgaaaga tatctccaga acgctgaaaa aagtctacaa
     2941 cgccgaatcg gcgattgtgg tgcccggcag cggcaccttc ggcatggaag cggtggcgcg
     3001 acaatttgcg actggcaaaa agtgcctggt gattcgaaac ggctggttca gtttccgctg
     3061 gagccagatt ttcgacgcgg gccatattcc cgccgagaca atcgttctga aggcgcgcac
     3121 cgtagaggca ggcaaccagg ccgcgttcgc tcccgcgcca atcaaagaag tggtggcggc
     3181 gatcaggcag cacaagccgg atgtggtgtt cgccgctcat gtcgagactg cctcgggcat
     3241 cttgctgcct gacgattaca tgcgtcagat tgcgcaggca gtgcatgccg aaggcgggct
     3301 gtttgtgctg gattgcatcg catcaggcac gctctgggtt gatatgaagg cgatcggggt
     3361 cgatatcttg attagcgcgc cgcagaaagg ctggagcggt cccgcgtgct gcgccctggt
     3421 gatgttgaac gcctcggccc gaaaaaagat ggaaaccacc accagcacga gctttgcctg
     3481 cgacctgcgc aagtggctgc agatcatgga ggcttacgaa aaaggcgggc atgcctacca
     3541 cgctaccttg cccaccgatt cgcttacggc cttgcgcaat gtaatggcgg aagcggaagc
     3601 ctatggtttt gaaaaggttc gcgccgagca acaagagctg ggcgacaagg tgcgcgccct
     3661 gcttcgcaaa aaaggtttca aaagcgttgc agcccctggt ttcgaggcgc cgggggtggt
     3721 cgtcagctac accagcgacg acgggattca ttcgaccaag agctttgttc aggcggggtt
     3781 gcaagccgca gcgggcgtgc cgctgcaatg cgatgaaccg caagggttca aaaccttccg
     3841 catcgggctg tttggccttg acaagctgca taacgtcgac aggacagtcg caacccttga
     3901 gaaggcactg gacaacatta cgcagcggga gcccgaaaaa gcgctccagt gacaggacga
     3961 cagctacttc gtccgcgcgt tgttcagggc gaagtagctg cgtatgacct ggctgtcatc
     4021 ggccaggccg tcgccgcgac ccgacgcggc cagaaatagc tgtgcagcgg ctgccgcaat
     4081 gggtagcgcg gcgcgtgcat ctttacctgt ttccagaacg atgcccaagt ccttcacaaa
     4141 gatatcaacc gtgctggtca cttcagggtc gtcctgcaac atgcgcggcc cacgatcatg
     4201 cagcatccag ctcgatgccg ccgagccttg cataatctcc agcagcaatt ccagatctat
     4261 tccggccttg gccgccaggc tgaaagcctc ggccattacg gcaatgtgta cgccgcaaag
     4321 caactggttc acagttttaa ccatggcgcc ctgtccggga gtcgggccaa tataaaaaac
     4381 acgctcgccc agtgcgttga agacgggctc gactgcatcg aaactcgcac gctcggccgc
     4441 cgccataata gtcaatgacc cggcgacagc gcctgcgacc ccgcctgata ccggcgcatc
     4501 gacaaagcgt ctgttggcgc ttgcgaccga tttgcttaaa ttttgcacgg cccccgaagg
     4561 gcacgttgcc atcaggcaaa cgactgcatt ggccgccagc ccatccagca cgccctgctg
     4621 gaccaagata ctctcagcct ggcttgcatt gaccaccatc aatacaagca cgtcggcgcc
     4681 acgtgtggca tcagccagcg acgtcacccg cactccgccc gactgcgcca acgcatcaag
     4741 cgccttgtcg ctcaggtccc aaccacgcac ctcaaacccc tttgacaaca agttcctcgc
     4801 catcggcaaa cccattgctc ccaaccccac aaaggccact acactcattt cggatactcc
     4861 atgcaaaatc aatcatgatc agcgcccgaa tgaggtcttc gagcctgacc tgcaattatc
     4921 caagccattg ccggatgcgc tcgcccaatg cctctgtgga aattccgtat ctgtcatgca
     4981 gcgtgggcaa ggcccccgcg tccaggaatg catcgggcaa cccggccaga cggaatcggg
     5041 gcgccacgcc ggactgcagc agcagcgagg ccaca
//
LOCUS       DATCXU010000515         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paralcaligenes sp. isolate SMAG_U7447 k141_456417,
            whole genome shotgun sequence.
ACCESSION   DATCXU010000515 DATCXU010000000
VERSION     DATCXU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955257
            Sequence Read Archive: SRR351474
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paralcaligenes sp. (soil metagenome)
  ORGANISM  Paralcaligenes sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Paralcaligenes.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,316
            CDSs (total)                      :: 2,293
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 17 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Paralcaligenes sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456417"
                     /isolate="SMAG_U7447"
                     /isolation_source="soil"
                     /db_xref="taxon:2005393"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.6380873 N 93.7753044 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1438)
                     /locus_tag="VIR04_11560"
     CDS             complement(92..1438)
                     /locus_tag="VIR04_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY9461426.1"
                     /translation="MNNWSSVNRLRRRSALKGALALAGGSLLSGSPFTALAASSKQAV
                     GNYPAGVKGDSAFVGIVVPLTGPYSSEGNDERRGYELAIEQLNSGAEEIRKISPLTKK
                     GVLGKTIKFGVADGETKPNSAIAAATRFVNENRAIMVTGAVSSSVSVALGHFLSREKT
                     LFIANFGSSNDVTGKDCQRYAFRLLCSAYAIAKAVAPAAAERLGHSRKAVYLVPDYTY
                     GHTLYESTRKFTEEEGWETVGVQFHPLGATDYSSYLINIANSGADTFVNCDYGGDATN
                     SVKQAKQFGVLGKMKMVVPQLSPFLADAVGADVMQEVIGVLVYWWTLQEQYPQSRDFV
                     SAFRKKYNDVPRGTAALAYLNMALWADAIERAGTFNPPDVIKAYEKQHKRDGLVGDVW
                     FRAADHQGVFNFPVLQGKKPGNMKDPKDFYNILHTVSGQSVVQPRDLFGCRLGAYS"
     gene            complement(1543..2229)
                     /locus_tag="VIR04_11565"
     CDS             complement(1543..2229)
                     /locus_tag="VIR04_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9461427.1"
                     /translation="MSSLIWTQVTTYAPFLWAGLEYSIRLTVVAAIAGLFLGTVLAVI
                     RILGWRIASRIVSFYVNVTRAIPLLLVIFTVYIFTPIFLQWVLGLRYPPKVSAANSAY
                     FTFALFEAAYYCEIIRAGINGVSKEQLNAAQALGLTKIQSMRFVVLPQALRVAIPMIV
                     TQIIILFQDASLVSLLNVIDFVGAANIIAERDGLLVEMYLIVAIVYLVICGALSLMVG
                     VLSKKFAIAK"
     gene            complement(2226..2951)
                     /locus_tag="VIR04_11570"
     CDS             complement(2226..2951)
                     /locus_tag="VIR04_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY9461428.1"
                     /translation="MGVLFEVSVDPTLPWWGFLLYGLGVSLAMVACAFVIALSVGSVV
                     GVLRTSRNRWIVRLSACYVFVFRNIPLLVQFFLWFYVVPGLSDSIKFLVLQTDPMVVQ
                     FVASVLCLGIYTSARIAEDVRSGIEALAGGQRQAAYAVGLNEWGVYRYVLLPVAFRII
                     IPQLTSETTSLVKNSSVAYSIGLAELFSRTLTMGELTFHFTTAFLASAVGYVSVTLTL
                     TMLMLALERKFRLVSSLASGPRT"
     gene            complement(2958..3875)
                     /locus_tag="VIR04_11575"
     CDS             complement(2958..3875)
                     /locus_tag="VIR04_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY9461429.1"
                     /translation="MRMSFKTVAAITSAAMIGMSGISTSFAADLTGTLQKIKETNSIT
                     LGHRASSMPISYMDDQHHPLGYAVDVCNMVVEDLKKELSLPNLQVHWQLTSAANRIPL
                     LRNGATDLQCGSGTITTARREVVSFGPAYYIAGITAIVKKSSGIKNMSDLRGKSVAVT
                     SGTTGVPVVTAYNEKHHLDLKLVAAKENGQSFLLLENDRVAAYMNNDIIVYGARAVTA
                     KNPDDYEVLPDPIDVEPFAVIFRKDDPQFKQFVDRSILSIIKSGEMEKLYNKWFLSPI
                     PPNNINLNMALGKLLAKQFKDPVADWVVK"
     gene            complement(4033..>4216)
                     /locus_tag="VIR04_11580"
     CDS             complement(4033..>4216)
                     /locus_tag="VIR04_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY9461430.1"
                     /translation="ASYGNVSLLFTSMRGDEIRQQTMSAEDMAEAQTQLAKLDDEALR
                     AMLAEAQPWNPGASGS"
BASE COUNT         1018 a         1173 c         1056 g          969 t
ORIGIN      
        1 catcggtgca ctaacgatcg gccagcggat atgtagctgg tcgcataacg gagtcttcag
       61 tgaagaaaac atggatcatt tccttaagat attaactata tgcgccgagt ctgcagccga
      121 ataaatcccg cggttgcacg acgctttggc cgctgacggt atgcaggatg ttgtaaaaat
      181 ccttcgggtc tttcatgttc cccggtttct tgccttgcag caccgggaaa ttaaataccc
      241 cctggtgatc cgctgcgcgg aaccagacgt ctccaaccag cccatcgcgc ttgtgttgct
      301 tttcgtaggc cttgatgaca tcgggagggt taaatgttcc ggcgcgttca atagcgtctg
      361 cccacaacgc catgttcagg taggcaagcg ccgcggtgcc gcgcgggacg tcgttgtatt
      421 tctttcgaaa cgcagaaacg aaatcacgcg attgcgggta ctgctcctgg agcgtccacc
      481 agtaaaccaa tactccaatg acttcttgca tgacatcggc tccaacggca tcggccagga
      541 acggcgacaa ctgtggcacc accattttca ttttgcccag gacaccaaac tgtttagctt
      601 gcttgaccga attcgtcgcg tcgccgccat agtcgcaatt gacgaatgtg tcggcgccgc
      661 tattcgcaat attgatcaaa tatgagctgt aatcggtggc gcccagcgga tggaactgca
      721 ccccgaccgt ttcccatcct tcttcctcgg tgaatttccg ggtcgactca tacaacgtgt
      781 ggccataggt gtaatccgga accaaataga ccgctttgcg tgaatggccc agccgctcgg
      841 cggccgcagg cgccaccgcc ttggctatcg cgtatgcgga acaaagcaag cgaaacgcgt
      901 agcgttggca gtccttgccg gtcacgtcgt tcgagctgcc gaaattggcg ataaaaagtg
      961 ttttttcgcg actaagaaaa tgtcccaatg cgaccgacac cgaactactt acggcaccag
     1021 tgaccatgat ggcccggttt tcattgacaa atcgggttgc cgccgcgatc gccgaattgg
     1081 gctttgtctc gccgtcggcg acaccgaatt tgatcgtctt gccgagcaca ccctttttgg
     1141 tcaaaggaga aatcttgcgg atttcttcag cgcccgagtt cagttgttca atggccaact
     1201 cgtacccacg acgctcatcg ttgccttcgg atgaatacgg gcccgtcagt ggaaccacaa
     1261 tgcccacgaa cgccgagtcg ccctttaccc ccgccgggta attgcccacc gcctgcttgc
     1321 tgctggccgc cagtgccgtg aacggcgagc ctgatagaag tgaaccgcct gccaaagcca
     1381 gcgctccctt gagcgcagat ctacgccgca gccgatttac gctgctccaa ttattcattg
     1441 tgctgtctcc gttttatatt tgtattcttg cgtctggact atcgatctca gcccttcgct
     1501 tcaaaaaggt tagatcgata ccctgtcaca gtcaaatgcg gcttacttcg cgatggcgaa
     1561 tttcttgctc aggactccaa ccataagaga cagagcgccg catatgacca gatagacgat
     1621 cgccacgatc aagtacattt cgacaagcag tccgtcgcgt tccgcgataa tgttcgccgc
     1681 gccaacgaag tcgatgacat tcagcagcga caccaacgag gcgtcttgaa acaggatgat
     1741 gatttgcgtg acgatcatgg gaatcgcaac acgtaaagcc tggggcagca caacgaaccg
     1801 catcgattga atcttcgtca gccccagcgc ttgggccgca ttcaactgtt ctttcgatac
     1861 cccatttatg cccgcgcgaa tgatctcgca gtagtatgcc gcctcgaata atgcaaacgt
     1921 aaagtatgcg ctgttcgcag cactgacttt gggtggataa cgaagaccca gaacccactg
     1981 tagaaagatg ggcgtgaata tgtacaccgt gaagatcacg agcagtaaag gaatcgcccg
     2041 cgtaacgtta acgtagaacg acacaatgcg cgacgcgatg cgccatccga gtatgcggat
     2101 cacggcaagc actgttccca gaaacaatcc tgcgattgcc gccaccactg tcagtcgaat
     2161 gctgtattcc agcccggccc acaaaaatgg cgcgtaggtc gtgacctgag tccagattaa
     2221 tgaactcatg tgcgtggccc gctggcaaga gacgacacca gacgaaattt gcgttcaagc
     2281 gccagcataa gcatcgtcaa cgtcaacgta acggacacat aacccactgc ggacgccaaa
     2341 aaggccgttg tgaaatggaa cgtaagctcg cccatggtca acgttcgtga aaataattcc
     2401 gcgagtccaa ttgaataggc gaccgacgaa ttctttacaa gacttgtggt ctcggacgtc
     2461 aattgcggga tgattatccg gaacgcaacc ggcagcagca cataacggta taccccccac
     2521 tcgttaagcc caaccgcgta ggccgcctgg cgctggccgc ccgcgagagc ttctatgccg
     2581 ctccgaacgt cttcagcaat tctcgcagat gtgtatatgc caaggcacaa tacggacgcc
     2641 acgaattgaa caaccatggg atccgtttgg aggacaagga actttattga atcggacaag
     2701 ccggggacaa cataaaacca cagaaagaac tgcaccagca acggtatatt cctgaacacg
     2761 aatacgtagc aagcgcttag tcgcacgatc cagcggttgc ggctggtacg cagcacgccc
     2821 accacagaac caacagaaag agcgatcacg aacgcacacg cgaccatagc cagacttaca
     2881 ccgagcccat agagtaaaaa cccccaccat ggcaaagtgg ggtctacaga cacctcaaaa
     2941 agcacaccca tgtgtgatta tttcaccacc cagtcagcca cgggatcctt gaactgcttt
     3001 gcaagcagct tgccgagcgc catattcaag ttgatgttat tgggcggaat cggcgagagg
     3061 aaccacttgt tataaagttt ttccatctct ccacttttga taatggacaa tattgaccga
     3121 tcaacgaatt gtttgaattg cggatcgtct ttgcggaaga tgactgcaaa cggctctacg
     3181 tcgatgggat cgggcagcac ttcataatcg tctggatttt ttgccgtgac ggcccgcgcg
     3241 ccgtacacga ttatgtcgtt gttcatgtag gcggcaaccc ggtcgttttc aagcaacagg
     3301 aatgactggc cgttttcctt ggccgccacc aatttaagat cgagatgatg tttctcgtta
     3361 tatgcagtca ccacaggcac ccctgtggtg cccgacgtaa cggcgacact tttgccgcgc
     3421 agatcggaca tgttctttat tcccgaactt ttcttgacga tggcggttat tccggcaatg
     3481 tagtaagcgg gcccgaacga aaccacctcg cgccgggcag ttgtgatcgt cccgctgccg
     3541 cactgcaggt ctgttgcgcc attgcgaagc aagggaatgc gatttgccgc gctggtgagt
     3601 tgccaatgca cttgaaggtt gggcagcgaa agttctttct tcaaatcttc aacgaccatg
     3661 ttgcatacat cgacggcata tccaagtgga tgatgctgat cgtccatgta agaaatcggc
     3721 atcgaagacg cccggtgacc caaagttatt gagttcgttt ctttaatttt ttggagcgta
     3781 ccagtcaaat ccgcagcaaa cgaagtggaa attccgctca tgccaatcat ggctgccgac
     3841 gttatggcgg caaccgtttt aaacgacatc ctcatatctt gtctccttgt gtattcagca
     3901 gtgccatttg cggcccagcg cttttatcca tacccgcttt tcttttagtt attgtcgata
     3961 aagttatcga taatatttca aatagtaaga accccaggga atatttgtct acctatggtt
     4021 aacccgtaaa gctcaagatc cgctggcgcc gggattccag ggttgggcct cggccagcat
     4081 tgcgcgtaat gcctcgtcgt ccagctttgc cagttgtgtc tgcgcctcgg ccatgtcctc
     4141 ggccgacatc gtctgctggc ggatttcgtc gccgcgcatc gaggtgaaaa gcagactgac
     4201 gttgccatat gacgct
//
